LOCUS       DATLCO010000001         3381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_746239, whole genome shotgun sequence.
ACCESSION   DATLCO010000001 DATLCO010000000
VERSION     DATLCO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746239"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VMZ33_00005"
     CDS             <1..204
                     /locus_tag="VMZ33_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR15639.1"
                     /translation="AWYGDRWARGAFAMFAPEQQTELQADIVAPEGRFYFAGEHCSLY
                     HAWIQGALESGIRAARQIHETPA"
     gene            272..658
                     /locus_tag="VMZ33_00010"
     CDS             272..658
                     /locus_tag="VMZ33_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUR15640.1"
                     /translation="MTPTKADVTSQLPAFRAWLTAFGDAWESGAAPEAAALFVVAATA
                     LPSPFADLLNGRRAIGDWLSGLFAAWPSARFTAQILGAGSTYGVAHWRVSSGSRAIDG
                     VLVCALDGRGRCTSLRMWFHEGRAVD"
     gene            695..886
                     /locus_tag="VMZ33_00015"
     CDS             695..886
                     /locus_tag="VMZ33_00015"
                     /inference="COORDINATES: protein motif:HMM:NF019057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HUR15641.1"
                     /translation="MKFVVRTNKGGEFFWQLVGGNGEVMAVSEGMSRKQSCLDSIESI
                     KRRAADADVVDKTEEGDAA"
     gene            1044..1538
                     /locus_tag="VMZ33_00020"
     CDS             1044..1538
                     /locus_tag="VMZ33_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HUR15642.1"
                     /translation="MNRVVLTGRLTRDPESRKLASGKTVTQFSVATNDYRGGTEKSEY
                     HQVVTWDRLAEICGQYLGKGQMVSVEGRIQTRQWEDDEKKRHWKTEIVAAAVEMLSGK
                     RKKDYAAESAAEQLIHQAQTFGIDPSSGEYHESDANAAAFVSLEGDEDEDEAEADETE
                     LVAA"
     gene            1826..2665
                     /locus_tag="VMZ33_00025"
     CDS             1826..2665
                     /locus_tag="VMZ33_00025"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HUR15643.1"
                     /translation="MLNSTRRTLLLSTTVATLLVGIIASPALAIRNGSLDGNAHPYVG
                     IMVAKDADGNPLWRCSGTLVSSTVFVTAGHCTESPAASAEIWFRSDMTNNALTGYPAN
                     GDYSGTTYTHPDYDPDNFAYRDLGLVILDQPAIMSQYAVLPGLNSLDSLKPSNGTTFT
                     SVGYGRQRSFPDAAAWKNQAVKVRMVAHPQLKQINTPNTGNYALLLVANASDGGTCFG
                     DSGGPNFLGDSNIIAAVTSFGWNSTCAGTSGVFRLDRSWSLAFINDAADGVMGDDYDG
                     GNS"
     gene            complement(2788..3234)
                     /locus_tag="VMZ33_00030"
     CDS             complement(2788..3234)
                     /locus_tag="VMZ33_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15644.1"
                     /translation="MPSPSAVVDELATVLFALDTGRRSTAGVTSQITAEIVRWAVTRG
                     WSARTEARVHAPDADHGDQRLGFLDVIVRRGNLLPDLAIEIDSTDKPWSVTKLRHAAQ
                     AGMVAIWVRWGDDDWAGIFDEIDVIQLPLIRRTGHRGTSNQPCFWP"
BASE COUNT          601 a         1079 c         1106 g          595 t
ORIGIN      
        1 gcctggtacg gcgaccgatg ggcgagggga gccttcgcga tgttcgcgcc cgagcagcag
       61 acggagctcc aggccgacat cgtcgctccg gaggggcgtt tctacttcgc cggcgaacac
      121 tgctcgctgt accacgcgtg gatccaggga gctctcgagt cgggcattcg ggcggctcgc
      181 cagatccacg agacgccggc ctaagccgcg ccgacacgcg gagctgtgta gtggacggcg
      241 agaaggtcat caatggcgtg tcgttcggcc agtgacgccg accaaggcag acgtcacatc
      301 ccagcttccc gccttccgcg cctggctgac tgcctttggt gatgcgtggg agtcgggcgc
      361 cgcgcctgag gctgctgcgt tgttcgtggt cgctgcaacc gcgctgccgt cgccattcgc
      421 tgacttgctg aatggccgac gggccatcgg ggactggctg tccgggttgt tcgccgcgtg
      481 gccctcggcg cggttcaccg cgcagatcct gggcgccggt tcaacctacg gcgtggcgca
      541 ctggcgggtt tcgagcggat cgcgtgcgat cgatggcgtg ctcgtctgcg cgcttgacgg
      601 gaggggccgc tgcacgtctc ttcgaatgtg gttccacgaa ggtcgtgccg tcgactaatc
      661 ttcgcgccgg ccaccgtacc caaggagaaa tgaagtgaag tttgtcgtgc gcacgaacaa
      721 gggtggggag tttttctggc agctcgtcgg tggaaacggc gaggtcatgg ccgtctcaga
      781 gggcatgagc cgcaagcagt cgtgcctgga ctccattgag tcgatcaagc gccgggccgc
      841 cgacgccgac gtcgtcgaca agaccgaaga gggcgacgcc gcttaattgc cgctaacgcg
      901 ccgcgagcga gttgagcgcg cagtaaccaa gcgataagcc cctggtaatc gcctccgcct
      961 atcctccgac gcgtttagtt cccgccgccc gccgataacg caggccgagc cgatcgatcg
     1021 gccggtcagg aggtatacgc ggcatgaacc gtgttgtgct cacgggacgg cttacgcgcg
     1081 accctgagtc gcgcaagctc gcttcgggca agacagtcac gcagttttcc gtggcgacca
     1141 acgactaccg cggtggcacc gagaagtcgg agtaccacca ggtcgtcacg tgggatcggc
     1201 tggcagagat ctgcggccag tacctgggca aggggcagat ggtgtccgtc gaggggcgga
     1261 tccagacgcg ccagtgggaa gacgacgaga agaagcgcca ctggaagacg gagatcgtcg
     1321 cggccgctgt cgagatgctg tcgggcaagc gcaagaagga ttacgcggcc gaatcagccg
     1381 ctgagcagct gatccatcag gcgcagacgt tcggcatcga cccttcctcc ggcgagtacc
     1441 acgagagcga cgccaacgcg gctgccttcg tgtcactcga aggtgacgaa gacgaggacg
     1501 aggccgaggc cgacgagaca gagctggtcg cggcctaacc cttccggtcg cggccggcgt
     1561 tcccagcgcg gcgggcttgg ctcctcctcg cccgccgcgc aacccttgaa tcgaccggca
     1621 gtctttctgg ctgatatgcg gatgcaacaa gttgcgtgta gctggataag ggctgaggag
     1681 tccggagcgc gtgtacggtc gggacgtacc aagtcgtcgc gacttcgcgc accggtagag
     1741 gcttggtcca cgtagaacgt tcgcccgcgg accagccgtg ccgttgggca accttctcgg
     1801 ccactgtcat tggggaggaa aacgcatgct caattcaacc aggcggacct tgctgctcag
     1861 cacgaccgtc gcgaccctgc tggtcggcat cattgccagc ccggcgctag cgatcaggaa
     1921 cggttcgctc gacggcaacg cccacccgta cgtcgggatc atggtcgcta aggacgccga
     1981 cggcaatcca ttgtggcgtt gctccggaac cctcgtctca tcgacggttt tcgtgactgc
     2041 cggtcactgc accgagtcgc ctgcggccag cgcagagatc tggttccgca gcgacatgac
     2101 caacaacgcg ctgacggggt atcccgctaa cggggactac agcggcacta cctacacgca
     2161 tcccgattac gacccggaca acttcgccta tcgagacctc ggcctggtca tcctcgacca
     2221 gccggccatc atgtcccagt acgcagtgct gcccggcctg aactcgcttg attcgctcaa
     2281 gccgagcaac gggacgacct ttacctcagt cggctacggc cgtcagcgca gcttcccgga
     2341 cgcggccgcg tggaagaacc aggcggtgaa ggtgcgcatg gttgcgcatc ctcagctgaa
     2401 gcagatcaac acgcccaaca caggtaacta cgcgctcttg ctcgtcgcca atgcgagcga
     2461 cggcggaacg tgcttcggtg attcgggtgg ccctaacttc ctcggcgact cgaacatcat
     2521 cgcggccgtc acgtcgttcg ggtggaacag cacgtgcgcc ggcacgtcag gcgtcttccg
     2581 cctcgaccgc agttggagcc tggcgttcat caacgacgcc gcggacggcg tcatgggtga
     2641 cgactacgac ggcggcaaca gctgaccgag tcgcgatcaa cctcgtcaat ctgaaagggc
     2701 cgccggcaac ggcggccctt tctttgtgct cggctcaagg gctcttggct atctcggaga
     2761 ctccaccgtc tcgtcggccc ggccacgcta aggccagaaa cagggctggt tggatgtgcc
     2821 tcgatgaccg gtgcggcgta tcagcggaag ttggatgacg tcgatctcat cgaagattcc
     2881 ggcccagtca tcgtcgcccc aacggaccca gattgcgacc atgcccgcct gggccgcgtg
     2941 acgcagcttt gtcaccgacc agggcttgtc tgtgctgtcg atctcgatcg ccaggtccgg
     3001 caacaggttg ccgcggcgga cgatgacgtc caggaagcca agccgctgat ccccgtgatc
     3061 tgcgtctggc gcgtggacgc gcgcctctgt tcgtgctgac cagccccgcg tcacagccca
     3121 acggacaatc tcggcggtta tctgcgacgt cactccggcc gttgaacgcc gtcccgtgtc
     3181 cagggcgaaa aggacggtag cgagctcgtc gaccactgcg ctgggagagg gcacgtggct
     3241 attcttggcc gatgccgtga cacctagcgt ggcgcaatca cccgctctgc agattccctc
     3301 ggatcaggtc ggcatggcgg tcaagatcgg ctcgtggtcg tgctcggtcg aagacctcgg
     3361 cggagatgat gaagccgtcg c
//
LOCUS       DATLCO010000002         9228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2238723, whole genome shotgun sequence.
ACCESSION   DATLCO010000002 DATLCO010000000
VERSION     DATLCO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238723"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VMZ33_00035"
     CDS             <1..340
                     /locus_tag="VMZ33_00035"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HUR15645.1"
                     /translation="DAIWRACRIILDIRLHRGELTVEEATDFLVEQTGFERPQAAAEV
                     NRYTYTPSYQLSYLLGKVLLLRLREDERRRLGSDFSLKSFHDALLREGSLPISFQRRV
                     LNGALTGSSA"
     gene            337..1068
                     /locus_tag="VMZ33_00040"
     CDS             337..1068
                     /locus_tag="VMZ33_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HUR15646.1"
                     /translation="MIVIPSIDLQTGRSRLVFWPGAASGTGTPTDRPEMIARHFVGLG
                     AAMLHLVDLDGATRGAPANTDAIQKISRSVAVPLQLAGGVDGPEQIELAFAAGATRVV
                     VPMWKVADSMETLRACLRVGGDWLAVGVDARSERLRDYPWRGAAPTFDQLVAMIATEG
                     VRRIVVSHWTGEHAAHLGALGRDLDLEVQLAGGVTDAAGVETARASGAHALIVGEALF
                     TGGVDYAAVAAPLATAGHVGGDARG"
     gene            1061..1885
                     /locus_tag="VMZ33_00045"
     CDS             1061..1885
                     /locus_tag="VMZ33_00045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUR15647.1"
                     /translation="MAKRRKSAPQRSHSVPSSDYRPGFSRRVDPKPPGERGPRPVIVL
                     GALLGVIAVLVAGAYFLGLLPGSGGAQSTPRPLRSPIGPVATLNPEGVHPPAATPMAS
                     PLAAPEGDGTRAIIEVPDGRIVLELYTESAPVASENFINLAEAGYYNGVSFHRIVPGF
                     VIQGGDPEGTGSGGPGYTIPDEKVVGTYGRGILAMARSQAPNSQGSQFFIVLSDSEDT
                     KRALSDVNTYVIFGKVVEGLDVVDTIAALPNDPNRGNQALVDYRMYSVTIVHPDEV"
     gene            2204..2279
                     /locus_tag="VMZ33_00050"
     tRNA            2204..2279
                     /locus_tag="VMZ33_00050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2237..2239,aa:Arg,seq:ccg)
     gene            complement(2292..3083)
                     /locus_tag="VMZ33_00055"
     CDS             complement(2292..3083)
                     /locus_tag="VMZ33_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUR15648.1"
                     /translation="MPRQRPQPEDLARDLVGAIERGEFRLEFQPVVRLGSGAVAGAEA
                     LLRWRHPQRGTISPNEFIPLADQAGLMDEIGAYVLDQACRAAAGWPRTADRPGFVSVN
                     VLPSQLRQPGIGERTLRMLSDAGLEPRLLTIELTSGAGHGHEQMAQVLTELRDAGIRV
                     ALDDFGMSTSLADLKLLPIDVVKLAHEFVADVTGPADDSVFATTLINLADSRAVEVVA
                     KGVESREQAWRLAELGCRYGQGFFFSKPLGASGVLAVLRQGSLRG"
     gene            complement(3139..3831)
                     /locus_tag="VMZ33_00060"
     CDS             complement(3139..3831)
                     /locus_tag="VMZ33_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HUR15649.1"
                     /translation="MSLAELEQINAEIVECRLCPRLVRWREEAARNPPARFADDDYWA
                     RPVPGFGDADASVVIVGLAPAAHGGNRTGRVFTGDRSGDFLFPVLHATGFASQPTSLR
                     IGDGLVVRDLYVVAVNRSAPPGNRPTPEERDNCLPYLVRELRALSRARVLVCLGAFAW
                     DGALRALAALGRQERPRPRFGHGAEARVGPYLMLGTYHPSQQNTFTGKLTSAMFTAIF
                     ERARVLAGLPPS"
     gene            complement(3828..5342)
                     /locus_tag="VMZ33_00065"
     CDS             complement(3828..5342)
                     /locus_tag="VMZ33_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUR15650.1"
                     /translation="MPEQTERPFPPGDYPVVVVGSGPGGLQASYFLGRLGIEHAVISA
                     DEGPGGMFRRFPFFQRLLSWTKPYGPVPRDDRFYEWYDWNSLLALEPENRSIMPGLMD
                     GSSSFPSRPEMELNLATFAERAGVRVRYGTTWEATRRDGERFVLETSAGEYRCKVAIF
                     AIGVAEPYTPDTPGFEHVAHYVDTRPADTYAGKRLFIAGKENSGFELATGLLQWAQRI
                     VLCSPRPAKLSVNTHSLLGVRARYIQPIEDQNLGGGVFLLNASINRVERTASGFRVNC
                     TRSEGGGEVNVDADEVIAATGFVCPLLDLPDLGVSVFSRSNLPSMTNHWESATVPGIY
                     FAGTIGQGVSGMKKYGMPANSGAVHGARYNARTMVYHVAQKHFGMEPERPTVEPTRLI
                     DTLLTEATHAPELWNQKSYLARAFAIDASSGISDEGVVPLAHFVDTEGSAAVAIAVET
                     DEAGDIHPAVYVREKGRPAVETLLMSDPLHDFRTSENRAALASALNGVTAGAVR"
     gene            complement(5362..5787)
                     /locus_tag="VMZ33_00070"
     CDS             complement(5362..5787)
                     /locus_tag="VMZ33_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUR15651.1"
                     /translation="MALSPSQLVDTLASLSLFADLSRPQVEVVAHKMTEESFPVGQRI
                     LRQGFTGTGFYVILDGEVAIKIDGQDVARLQKGDFFGEMSVLLGEPPVADVVALRQLH
                     VLHLGGPELEGFLRDHPAVMYRMLKTMALRLYNSSRTQS"
     gene            complement(5787..6215)
                     /locus_tag="VMZ33_00075"
     CDS             complement(5787..6215)
                     /locus_tag="VMZ33_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUR15652.1"
                     /translation="MTLTAESKANALSSVPLFAGISPESLQKLADVTGEATFEPGQFI
                     VIQGQVGTGLYVIVEGSAKVARGSDELAVLGAGEFFGELSVIDQQPRYASVQALEPTR
                     CLALASWDLLDLIEKDPQLAQNLLRGMAQRIRAAGDQHRH"
     gene            complement(6238..6552)
                     /locus_tag="VMZ33_00080"
     CDS             complement(6238..6552)
                     /locus_tag="VMZ33_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HUR15653.1"
                     /translation="MSSDEVPGQERLTATVRGRVQGVGFRWFVRTRAESLGLRGWTAN
                     QSDGSVIIVAEGPTQVLHELLAVLERGPSGASVHGVDADRTSATGEFKGFKIRHGAHR
                     GD"
     gene            complement(6549..7691)
                     /gene="holB"
                     /locus_tag="VMZ33_00085"
     CDS             complement(6549..7691)
                     /gene="holB"
                     /locus_tag="VMZ33_00085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HUR15654.1"
                     /translation="MSRLRTRGHRTAVAFVVRAIATEQPPHAVLFVGPDGVGKTTLSL
                     DLAAGLLCLAADSSQRPCRECAACRKVDHLNHPDLHRLAPEGAGQQIRVAQVHELVSQ
                     LALLPLEGRFRIAIVEHAQRLNLDAQNALLKTLEEPPGRACLILAADDSTALLATVVS
                     RCARLRLGTLDDETIGEMLTVAGLADRASGAALARVAGGRPGVALALARHPEAVLAHG
                     RLVRTLIDLVRADRRTRLAAQADLIEGGAVLAAAAGSQSSDGDDQAQQPKPARRRTAT
                     PERTTAARRPSPAERRGAVAVVISVWHDVARDLAVAAKGGRRELRQVDLLDDLVRAAA
                     NTDPAELVAFMARLDGVGRALDAYANPELALDALLLAWPRQRAAAA"
     gene            complement(7704..>9228)
                     /locus_tag="VMZ33_00090"
     CDS             complement(7704..>9228)
                     /locus_tag="VMZ33_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15655.1"
                     /translation="RGNTSSDLIEVADQFTDIAQIARVDKSSGRSGRRVAAEGDLSMT
                     EVPEKETEGAPRRRRGGGRTRDEREYGEVAPVAAVPAATAVPASNLVVLPGERLSRAP
                     EGEEGADEGELVAAAADLDPDAVPGESEEARRRRRRRGGRGRGRGRRGETETYGVASN
                     GETAPTVESDDDEEFEELVPSQPPHQTFGSVWDSQIGVPSGPSANTFASFQPTAEGVA
                     GEDEDLDEPEVPEYLLAERRQRGQRPPAGRGGSGGRMQRGNRAAYQVALDRERFGRGA
                     GSAPTPSGFGANSGSSGGNSGSGGGGAGRPDRQDRGRDRNRGRDRYAQGRSQDRAPRN
                     DVQPYNAERSSAEPWSEVPPELEQMLRAELERKTGASGARPSREAPAFEQRAEPASAP
                     TFSGEPEAGQPAETAPAKPARRARTTKAVSTSATPEAPTEEAVPADESGATRKAAAPR
                     KRSTTPTTTRSRTTTKAKATQAASEESAAAEEPAAEAEPKKAAARRRRTTTSATEES"
BASE COUNT         1443 a         3131 c         3142 g         1512 t
ORIGIN      
        1 ggacgccatc tggcgggcgt gccggatcat cctcgacatt cgcctgcatc gcggcgagct
       61 caccgtggag gaggcgaccg acttcctggt tgagcagacg ggcttcgagc gaccgcaggc
      121 tgcggccgag gtcaatcgct atacgtacac gccgagctat cagctgagct acctgctggg
      181 caaggtcctg ttgctccgac ttcgtgagga cgagcggcgt cgcctgggca gcgatttctc
      241 gttgaagagc ttccacgatg ccctgctgcg tgagggcagc ctgcccatca gcttccagcg
      301 acgggtgctg aatggcgccc tgacggggag cagcgcttga tcgtcatccc gagcatagat
      361 ctgcaaaccg gcaggtcgcg actcgtcttc tggcccgggg ccgcgtcggg cacgggcacg
      421 cccacggacc gccccgagat gatcgctcgt cacttcgtcg gcctcggggc cgcgatgctt
      481 catctcgtcg acctcgatgg agcgacgcgc ggcgctccgg caaacaccga cgccatccag
      541 aagatatcgc gctccgttgc cgtgccactt cagctggcgg gcggcgtcga tgggccggag
      601 cagatcgagc tcgccttcgc ggcgggcgcg acccgcgtgg tcgtgcccat gtggaaagtc
      661 gccgactcga tggagaccct gcgcgcgtgc ctgcgtgtcg gcggcgactg gctggcggtc
      721 ggcgtggatg cgcgatccga gcgtctgcgc gactatccgt ggcgcggtgc cgcgccgacg
      781 ttcgatcagc tggtggcgat gattgccacc gagggtgtcc gcaggatcgt cgtatcgcat
      841 tggactggtg aacacgcagc gcatctcggc gcgctgggac gcgacctaga cctggaggtt
      901 caattggctg gaggggtaac cgatgccgcg ggcgtcgaaa ccgcacgtgc gtcgggtgca
      961 cacgcattga tcgtgggtga ggcgctcttc acgggcggcg ttgactacgc ggctgtcgcc
     1021 gcgccactgg cgaccgcagg ccatgtcgga ggcgacgccc gtggctaagc gacgcaagag
     1081 cgcaccgcag cgatcacata gcgtgccgtc gtccgactat cggccgggct tctcgcggcg
     1141 cgtggatccc aagccaccgg gcgagcgtgg tccgcggccg gtcatcgtgc tcggcgcctt
     1201 gctcggcgtc atcgccgtgc tcgtggcggg cgcctacttc ctgggcctgt tgcccggctc
     1261 gggcggcgcg cagagcacgc cgcgacccct gcgctctccc atcgggcctg tcgcgacgct
     1321 caatccagag ggtgtccacc cgccggccgc gacgccgatg gccagcccgc tggccgcacc
     1381 tgagggtgac ggcacgaggg cgatcattga ggttccggac gggcgcatcg tcctggagct
     1441 ctataccgag tcggcgccgg tcgcgtctga gaatttcatc aatctggcgg aggcgggcta
     1501 ctacaacggc gtgagcttcc accggatcgt gcccggcttc gtcatccagg gcggcgatcc
     1561 cgaaggcact ggatcgggcg gcccgggcta cacgatcccc gacgagaagg tcgtgggcac
     1621 ctacggacgg ggcatcctcg ccatggcacg ctcccaggca ccgaattcgc agggatcgca
     1681 gttctttatc gtcctcagcg acagcgagga cacgaagcgt gcgctatcgg acgtcaacac
     1741 gtacgtcatc ttcgggaaag tagtcgaggg cctggacgtg gtcgacacga ttgccgcgct
     1801 gccgaacgat cccaaccgcg gcaaccaggc gctcgtcgac taccgcatgt actcggtaac
     1861 gatcgtccac ccggacgagg tctgattagc gcccacgctc aaataaccgt tatgggttct
     1921 taacaatcgc tcgtggataa ccccttgagg cgcctaaagg ggtcaggtaa actacgggct
     1981 ggatgtcgcc tagtgcggca tgtgctgccc caggcgcaag gtcatgggta ggttgggtta
     2041 gggtagccgg tgcagccggc gtgatagatg cccttgtggc gtggcagggc tcagtgactt
     2101 cggttactgg atcggccggg acagtgcggc ggcccctcga aagaggggcc gcttctttat
     2161 gtccccgcgg ttatcgccat ctcctgcggt agtctcactc agagcgcccg tagctcagcg
     2221 gatagagcgt ctgcctccgg agcagaaggc cgtgggttcg agtcccgccg ggcgcgccac
     2281 tcgccggtgg gttagccacg cagtgagcct tggcgaagta ccgcaaggac gccgctggca
     2341 cctagtggct tgctgaagaa gaaaccctgg ccgtagcggc accccagctc ggcgagtcgc
     2401 cacgcctgct cgcgcgactc cacgcccttg gccaccactt cgacagcccg tgaatcggcg
     2461 aggttgatga gggtcgtcgc aaaaaccgag tcgtctgctg ggccggtcac gtcggccacg
     2521 aactcgtgcg cgagcttcac gacatcaatc ggcaagagct tcaaatctgc cagcgacgtc
     2581 gacatgccga agtcgtcgag ggccacgcgg atgcccgcat cgcgcagctc ggtcagcacc
     2641 tgcgccatct gctcgtgccc gtgcccggcg ccgctcgtca actcgatggt gagcaaccgc
     2701 ggctcgagtc cggcgtccga aagcatccgc aacgtgcgct cgcctatgcc gggttgacgc
     2761 agttgactcg gcaggacgtt gacgctgacg aaccctggtc gatcggccgt gcgcggccat
     2821 ccggccgccg ctcgacaggc ctgatcgagc acgtacgccc cgatctcatc catgagaccc
     2881 gcctggtcgg cgaggggaat gaattcgttt ggcgaaatcg tgccgcgttg tggatggcgc
     2941 catcgcagga gcgcctcggc cccggccacg gcgcccgatc cgagacggac gacaggctga
     3001 aactcgagcc ggaattcgcc acgctcgatt gccccgacga gatcgcgggc tagatcctct
     3061 ggttggggcc gctgtctcgg catcggcaaa cgataggcca atgagggtgg ccttgtgtgg
     3121 cccgagcggc ctcggcaact atgagggcgg cagtcctgcg agaacgcgcg ctcgttcgaa
     3181 gatcgccgtg aacatcgccg acgtgagctt gccggtgaag gtgttctgct ggcttgggtg
     3241 ataggtaccc agcatcaggt agggccctac ccgagcctca gcaccgtggc cgaaccgcgg
     3301 tcgtggccgc tcctggcgcc caagcgcagc gagcgcgcgc aacgcgccgt cccaggcgaa
     3361 ggcgccgagg catacgagga cgcgggcccg gctcaacgct cgaagctcgc ggaccaagta
     3421 gggcaggcag ttgtcgcgct cttcgggagt cggacggtta cctggcggag cactgcgatt
     3481 gaccgccacg acgtagagat ccctgaccac caggccgtcg ccgattcgca gggatgtcgg
     3541 ctggctggcg aagcccgtgg catgcaggac agggaacagg aagtcgcccg atcggtcgcc
     3601 ggtgaagacg cgcccggtcc ggttgccacc atgtgccgcg ggcgccaggc cgacgatgac
     3661 gacgctcgcg tcagcgtcgc cgaaacccgg gacaggccgc gcccagtagt cgtcgtcagc
     3721 gaaccgggcg ggtgggttcc gcgccgcctc ttcgcgccag cgaaccagcc gcgggcagag
     3781 ccggcactcc acgatctcgg cgttgatctg ctcgagctcc gccaggctca ccggaccgcg
     3841 ccggccgtca ctccgttcag ggcacttgcc agggcggcgc ggttttcgga ggtccgaaag
     3901 tcgtgcagcg ggtcagacat gagcagcgtc tcaaccgccg gacggccttt ttcacggacg
     3961 tagaccgcag gatggatatc gcccgcctcg tccgtctcga cggcgatcgc cacggccgct
     4021 gaaccctccg tgtcgacgaa gtgcgcaagt ggcacgacgc cctcgtcgct gatgccgctc
     4081 gacgcatcga tcgcgaaggc gcgcgccagg tacgacttct ggttccacag ctcgggcgcg
     4141 tgcgtggcct cggtcaggag cgtatcgatg agccgtgtcg gctctaccgt cggccgctct
     4201 ggctccatgc caaagtgctt ctgggccacg tggtacacca tcgttcgcgc gttataacgg
     4261 gcaccgtgaa cagcgcccga gttggccggc atgccgtact tcttcatgcc cgacacgccc
     4321 tgaccgatcg tgcctgcgaa gtagatcccg ggcacggtcg cactctccca gtggttcgtc
     4381 atcgacggca ggttgctgcg cgagaacacg ctcacgccca ggtcgggcag gtccagtaac
     4441 gggcagacga agccagtggc cgcgatgacc tcatcggcgt cgacgttgac ctcgccgccg
     4501 ccctccgagc gcgtgcagtt gacgcggaac ccggatgcag tgcgctccac acggttgatc
     4561 gaggcgttta acaggaatac gccgccgccg aggttctggt cctcgatcgg ctggatgtac
     4621 cgcgctcgca cgccgagcag cgaatgggtg ttgaccgaca gcttggccgg ccgcggcgag
     4681 cacaggacga tgcgctgggc ccactgcagt agcccggtgg ccagctcgaa gcctgagttc
     4741 tccttgcccg cgatgaacag cctcttcccg gcatacgtgt cggcagggcg cgtgtccacg
     4801 tagtgggcaa cgtgctcaaa gccgggcgtg tccggtgtgt agggctcagc gacgccaatc
     4861 gcgaagatcg caaccttgca ccggtactca cccgccgacg tctcgagcac gaagcgctcg
     4921 ccgtcgcggc gagttgcttc ccaagtcgtg ccgtagcgga cgcgcacccc ggcacgctcc
     4981 gcgaacgtgg ctaggttgag ctccatctcc ggtcgcgacg ggaacgacga cgagccgtcc
     5041 atcaggccgg gcatgatcga gcgattctca ggctccagcg ccagcaggga gttccagtcg
     5101 taccactcat agaaccggtc gtcgcgcggg acggggccat atggcttcgt ccaggaaaga
     5161 agacgctgga agaatgggaa tcggcggaac atcccgcccg gtccttcatc ggcagagatg
     5221 accgcgtgct cgatgcccag ccggccgaga aagtagctcg cctgcagccc acccgggcca
     5281 ctgccgacta cgacgaccgg atagtcgcct ggcggaaacg ggcgctctgt ctgctcgggc
     5341 acgtcggctt gtgaggtcgc gtcagctctg ggttcgactc gagttgtaga gccgcagcgc
     5401 catggtcttg agcatccggt acataacggc cgggtgatct cgcaggaacc cctcgagctc
     5461 gggcccaccc agatggagca cgtggagctg ccgcagggcc acgacgtcgg ccaccggcgg
     5521 ttcgccgagg aggaccgaca tctcgccgaa gaagtcgccc ttctggaggc gagcaacgtc
     5581 ctgaccatca atcttgatcg cgacctcccc gtcgaggatc acgtagaagc ccgttccggt
     5641 gaagccctgg cggaggatgc gctggccgac gggaaacgac tcctcggtca tcttgtgggc
     5701 cacgacctcg acctgcggcc gggacaggtc ggcgaagagc gacaggctcg cgagcgtatc
     5761 gactagctgg gaagggctga gcgccatcag tgccggtgct ggtcacccgc cgcgcggatg
     5821 cgctgagcca tgccgcgcaa caggttctgg gcaagctgcg ggtccttctc gatgaggtcg
     5881 agcaggtccc atgaggcgag ggccaggcag cgcgtcggct cgagtgcctg aacgctggcg
     5941 tagcggggct gctggtcgat gaccgacagc tcgccaaaga actcgccagc gccgagcaca
     6001 gccagctcat ccgagccacg tgcgaccttg gcggacccct cgacgatcac gtagagaccg
     6061 gtacccacct gaccctggat cacgatgaac tgacctggct cgaacgtcgc ctcgccggtt
     6121 acgtcggcaa gcttttgaag cgattcgggg ctgataccgg cgaacaacgg cacggacgac
     6181 agggcgtttg ccttgctctc agccgtcagc gtcatgcgct gcacgatagc agcgccgtca
     6241 gtcccctcgg tgcgcaccat gacggatctt gaatcccttg aactccccgg tagccgatgt
     6301 ccggtctgcg tccaccccgt ggaccgacgc accggagggg ccacgctcaa ggactgccag
     6361 cagctcatgg agcacctggg tcggaccctc cgcgacgatg atgaccgaac cgtccgattg
     6421 attcgccgtc caccccctca gccccaggct ttcggcccga gtccggacga accagcggaa
     6481 accgacgccc tggacccggc ccctaacggt ggccgtgagt cgctcctgcc ccggcacctc
     6541 gtcgctgctc atgcagccgc tgcacgttgg cgcggccacg ccaggaggag cgcgtcgagc
     6601 gccagctcgg ggttggcgta ggcgtcgagt gcacggccca cgccgtcgag gcgtgccatg
     6661 aacgctacga gctcggccgg gtcggtgttc gccgcggctc gaacgagatc gtcgaggaga
     6721 tcgacctgac ggagctcgcg ccggccgccc ttcgcagcga cagccaggtc acgtgcaacg
     6781 tcgtgccaca ccgagatgac gacggcaacc gcgccgcgcc gctccgccgg gctgggccga
     6841 cgggctgccg ttgtgcgctc gggggtggct gtgcgtcgcc gcgcaggctt gggctgctga
     6901 gcttggtcgt cgccgtcaga actttggctt cccgcggcag ccgcgaggac cgcgccgccc
     6961 tcgatgaggt cggcctgtgc ggccaggcgc gtgcgccgat cggcgcgtac gagatcgatg
     7021 agcgttctga cgaggcggcc gtgagcgagc acggcctcag ggtgacgcgc cagggcgagc
     7081 gccacgcccg gtcgcccgcc ggcgacacgc gccagggcgg cgccgcttgc cctgtccgcg
     7141 agaccggcaa ccgtgagcat ctcgccaatg gtctcgtcgt cgagcgtgcc cagccgcagg
     7201 cgcgcgcagc ggctcacgac ggtggctagc agcgccgtgg aatcgtccgc ggcgaggatc
     7261 aggcaggcgc gccccggtgg ctcctcgagc gtcttgagca gcgcgttctg cgcatccaag
     7321 ttgagccgct gagcatgctc gacgatcgcg attcgaaagc gtccctcgag cggcagcaac
     7381 gccagctgcg aaacgagctc atggacctgg gccacgcgga tctgctgacc ggcgccttcc
     7441 ggcgcgagtc gatgaaggtc gggatgattg agatggtcga ccttccggca tgccgcgcac
     7501 tcccggcacg gccgctggga cgagtcggcc gccaggcaaa ggagcccagc ggccaggtcg
     7561 agcgacagcg tcgtcttgcc gaccccgtcg gggccgacga acagcaccgc gtgcggcggc
     7621 tgctcggtgg ctatggcacg gacgacgaac gcgaccgccg tcctgtggcc gcgcgtacgg
     7681 aggcggctca cgccctcgga gccctaggac tcctcggtcg cgcttgtcgt ggttcgtcgg
     7741 cgacgtgcag ctgccttctt gggctcagcc tcggccgccg gctcttcagc ggcagccgat
     7801 tcctcggatg ctgcctgcgt cgccttggcc ttcgtcgtcg tccgcgaccg cgtggtcgtc
     7861 ggcgtcgtcg aacgcttgcg tggggccgcg gccttcctgg ttgcacccga ctcatcggcc
     7921 gggacggcct cttccgtggg tgcctcaggc gttgccgatg tcgaaacagc cttcgtggtc
     7981 cgagcgcgtc gggccggctt ggccggggcc gtctcggctg gctggcccgc ttcgggctca
     8041 cctgagaacg tcggcgcgct agctggttcg gcacgctgct cgaaggcggg ggcctcgcgg
     8101 ctgggtcgag caccggacgc accggtcttg cgctcgagct cggcacgcag catctgctcg
     8161 agctcgggcg gaacctccga ccagggctcg gccgacgagc gttcggcgtt gtaaggctgc
     8221 acgtcgttgc gtggcgctcg gtcctgcgaa cggccctgtg catagcgatc ccgaccgcga
     8281 ttgcggtcgc ggccacgatc ctggcgatcg ggtcggccgg caccaccgcc gccgctgccg
     8341 ctattgccgc cgctgctgcc gctgtttgca ccgaagccac tcggggttgg tgcagagcct
     8401 gccccgcgac cgaagcgctc acggtcgagt gctacctggt acgccgcccg gttgccacgc
     8461 tgcatgcggc cgccgctgcc accacgcccg gcgggcgggc gctggccacg ttggcgtcgc
     8521 tcagccagca gatactccgg cacctcgggc tcgtccagat cctcgtcctc gccggctacc
     8581 ccttcggcag tcggctggaa cgatgcaaag gtgttggctg atggccctga gggaacccca
     8641 atctggctat cccagaccga gccgaacgtc tggtgcggcg gctgtgacgg tacgagctct
     8701 tcgaactcct cgtcgtcgtc gctctccacc gtcggcgccg tttcgccgtt gctggcgacg
     8761 ccgtatgtct cggtctcacc acgtcggcca cggccacggc cgcgaccacc gcggcgccgg
     8821 cgccgtcggc gggcctcttc cgactcgccg ggaactgcgt ccggatccag gtccgccgca
     8881 gctgcgacga gctcgccctc gtcggcacct tcttcacctt caggcgcgcg cgacagtcgc
     8941 tcgccgggca agacgacaag gttgctggcc ggtacggccg tcgcggcggg gacggcagcc
     9001 actggcgcca cctcgccgta ctcgcgttcg tcacgcgtcc ggccgccgcc acgacggcga
     9061 cgcggagcac cttctgtttc tttctccgga acctccgtca tcgacaggtc gccttcggcg
     9121 gcgacgcggc ggccgctgcg gccagagctc ttgtcgacgc gcgcgatctg ggcgatgtcg
     9181 gtgaactggt cggcgacctc gatgaggtcg gacgaggtgt tgccgcgg
//
LOCUS       DATLCO010000003         7790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4475171, whole genome shotgun sequence.
ACCESSION   DATLCO010000003 DATLCO010000000
VERSION     DATLCO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4475171"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VMZ33_00095"
     CDS             <1..589
                     /locus_tag="VMZ33_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15656.1"
                     /translation="GRTRSMNKFAATAITSGMMAIAVACGGGSAPTRASVAPSLTGGI
                     PHPPSAAAPSGPSGDLATPEGLCGLLTAADWQQFDYVTAAQPDVSSDGEGTAICTWAN
                     GLTLEVYTHADASEAEETFATVVENVPMDDPQPFYVPGSAQSVFDSDIGDDSAGMVAQ
                     AGRLTVFVSGLSRDTAQAELAALAGLVIARGSSLK"
     gene            608..1072
                     /locus_tag="VMZ33_00100"
     CDS             608..1072
                     /locus_tag="VMZ33_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15657.1"
                     /translation="MDFERLAPVVIGAVFGALGWLFVGLYMQRKANDRLARNAGRAVY
                     FELAMNEIDVEVASTHSVFGQLRRSAFDRLLPELATWLEPDQLETVVRAYMSHAGYDQ
                     VQRDEKLPHPVKVALLKRVLDEHESAATLLRQRVFSAKEQARLGTLVRANHG"
     gene            1096..1548
                     /locus_tag="VMZ33_00105"
     CDS             1096..1548
                     /locus_tag="VMZ33_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUR15658.1"
                     /translation="MPVSKSEFFTRYVKAIADRDFDALEGMIHRDYVGEYPQSGERFT
                     GFPAFRAQLENYPGGLPAARSDDPNTKILGDDERWAISPGYTVLPLAGPERYTTVTRA
                     PYPDGSRWWVVSILTLKDDRVAHAETYFAPEFEPPEWRKDMVEVVTRD"
     gene            1719..3350
                     /gene="guaA"
                     /locus_tag="VMZ33_00110"
     CDS             1719..3350
                     /gene="guaA"
                     /locus_tag="VMZ33_00110"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HUR15659.1"
                     /translation="MLAPDDAEVEAYLEVALDAPAPQRPVLDPPTPDNVIVLDFGGQT
                     AQLIARRVRELNVYSELMPFDTPWEDILRRRPSAVILSGGPMSVYEDGAPHPDPRIWG
                     STDIPVLGICYGVHLMAHHLGGEVIPAGKREYGPATVSITTEDSLFKGLDKQQAVWMS
                     HGDSIVTPPPGFTPLAETESTPYAGLADTARRMYGIQFHPEVVHTAAGRDILRNFVLG
                     IAGATPSWTSASFIESTIADIRARVGDGKVICALSGGVDSAVAATLVHRAVGDQLTCI
                     YVDHGLMRKRESELLRQTFEQNLGMNLVMVDATERFLRRLMEVSDPESKRRIIGDEFI
                     RVFEEEATKLGQIDFLTQGTLYPDVIESATPETKAAQKIKTHHNVGGLPSDLRFQLIE
                     PLRYLFKDEVRTVGLELGLPETMVHRQPFPGPGLAIRIIGEVTKERLDILREADWIVI
                     DEIKAAGLYRSLWQSFAILTPVRSVGVMGDGRTYANVVAVRAVTSEDGMTADWAKLPY
                     DVLAKISSRIVNEVPDVNRVVYDISSKPPATIEWE"
     gene            3447..3920
                     /locus_tag="VMZ33_00115"
     CDS             3447..3920
                     /locus_tag="VMZ33_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15660.1"
                     /translation="MTRVPAPGISYIKEEAVTLRGPKGEPGERLSAGMTNPSVMDDGW
                     WLTHLWVADGEGVVDAATVAPAAGPPPGTPLATLGPRVAGSLSGLIAEEDGRQLIRLR
                     MPEAPDESRPWERPLLCMVAIRWDPVRASVITRNQLARELLRGFAAAVEGLGRPG"
     gene            3953..4795
                     /locus_tag="VMZ33_00120"
     CDS             3953..4795
                     /locus_tag="VMZ33_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15661.1"
                     /translation="MDVTLAPERLFVLEERLTVDEIQQRAMDKRTQAFGGGLGAFLQR
                     PKTDEVALVAKQRRLEPFWHVRGKATYVYERNRSYTVPAGSADVEAVTINGTRYDGAP
                     APQRTFAVPVLEHCRATFTSESFTDGLTGAAVPEGSALITGVRSEVGDPQTLTANETL
                     AEPPEHRASFVVRQVMSQIMKPVQADRVLEEAVELEVTDLYYRPWWAFEFTWQGKGKQ
                     GVVEIDAITGNTRQGKPLTGQIRSMFNRDVLFDVGADTVGLFVPGGSIAVKIAKAAID
                     SNKK"
     gene            4891..5799
                     /locus_tag="VMZ33_00125"
     CDS             4891..5799
                     /locus_tag="VMZ33_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15662.1"
                     /translation="MAVVAGQPRLWLLGAAGFLLRGGIVVLIAPVLVVPTQVEIRLLL
                     GDYLGTSGITPSFYPLVIGIAVVAAVVLLGLVLVLARLELGLFDALSTDERVAAHVGD
                     WQPIARGEASRGTLFARLVTVQVWTVIAILIAALPLALAIGETAYAEVIRPTSPAPIY
                     QRVLGAVTVPFMLFVGAFLAIEALSAVISRRLLVGRRSSLGLIGAATSGLRRVIRSPL
                     RTLAAALSGWALALVALTPVLAIIVVTWQPVRRAFLSSVSFGDVVEPRTLMAAIVLAG
                     SFVIGLVVAGFVSAVRSAIWTVDSVS"
     gene            5962..6633
                     /locus_tag="VMZ33_00130"
     CDS             5962..6633
                     /locus_tag="VMZ33_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUR15663.1"
                     /translation="MIDQLLDTLGSEIGKIFDSPLVRLSLQAIGFYVVLLWAATAYWA
                     YRDLQTRTTNPVAPYLAAALIILFTPVFFLFGVIVYRILRPSETVAEANERALAEEAM
                     MVEIESQPHCANCSRQVHEDWIICPTCRNRLRRVCPNCSRLVELDWSLCAWCGKDFER
                     PELLQDVVLPVPSGKQARQAADTYIDQPTDTQFEPPAPVPQIASSAAENRVPERAPRA
                     APTEN"
     gene            6753..7637
                     /locus_tag="VMZ33_00135"
     CDS             6753..7637
                     /locus_tag="VMZ33_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HUR15664.1"
                     /translation="MGVGGFVVLLIAAGAPPPWGGLLFMAALLATTLGLGAAAGYQLI
                     ARVTRAASAFRGPSPAILLALQLIVSIALGTAWVVLGVPDPQSSSVGFLIIAATLLVT
                     YVGIVWLFAVRSGVLSWSEMGWPTRAPASRLLGDIGVGFAAMLVAWPVVTFLAALLGT
                     LLQSTPTNPVPSIATTTDTILTALGAALLVPIGEEVLFRGYALTAWLRDLGPRTALIR
                     STIFFAFAHILGVTAASFDEGWRQALLTVVVITPVGALLGVLFLRRGLLAAIAGHVTF
                     NLVSVLAMALAQSLPPPQ"
BASE COUNT         1224 a         2585 c         2563 g         1418 t
ORIGIN      
        1 agggaggaca cgctcgatga acaagtttgc agccacagcg atcacatcgg ggatgatggc
       61 catcgcggtc gcttgcggtg gtgggtccgc cccaacgcgt gcaagcgtcg ctccgagcct
      121 caccggtggg ataccgcatc cgccttccgc cgcggctccg agcggaccat cgggcgacct
      181 ggcaaccccg gaaggcctct gcggattgtt gaccgccgcc gactggcagc aatttgacta
      241 cgtcactgcg gcgcagcccg acgtctcgag cgatggcgaa gggacggcca tctgcacgtg
      301 ggccaacgga ctgacccttg aggtctacac gcacgccgac gcgagcgagg ccgaggagac
      361 gttcgccacg gttgttgaaa acgtcccaat ggacgacccc caaccgttct acgttccggg
      421 gtcagctcaa tcggtgttcg actccgacat cggcgacgac agcgcgggga tggtggctca
      481 agcggggcgg ctgactgtgt tcgtctcagg cttgtcgcgt gacacggctc aggcggagct
      541 ggcggccctg gcgggcctgg tgatcgcccg cggctcttcc ctgaagtgat cgtgagccgt
      601 taggccgatg gatttcgagc gtctcgcgcc cgtcgttatc ggtgcggttt tcggcgctct
      661 cgggtggctc ttcgtggggc tgtacatgca gcgcaaggcc aacgatcggt tggcacgcaa
      721 tgccggccgg gcggtctatt tcgagctggc aatgaatgag atcgatgtgg aagtggcaag
      781 cacgcattcg gtcttcggac agctacgacg aagtgcgttc gaccgccttc ttccggagct
      841 tgcgacctgg ctcgaaccgg accagctgga gacggtcgta cgggcgtaca tgagccacgc
      901 tggatacgac caggtgcagc gcgacgagaa gcttccgcac cccgtcaagg ttgccctgct
      961 gaagcgcgtg ctcgacgagc acgaatcggc agccactctt ctgcgacagc gcgttttcag
     1021 cgcaaaggaa caggcccgac tagggactct cgtgcgcgcg aaccatggct gagcccacac
     1081 aaccagaggt cgactgtgcc ggtatctaag agcgagttct tcacgcgcta cgtcaaggcg
     1141 atcgccgatc gcgactttga cgcgctcgag ggcatgatcc atcgcgatta cgtgggcgag
     1201 tatccgcaat ccggtgagcg tttcactggc tttccggcgt tccgcgcgca gctcgagaac
     1261 tatcccgggg gcctcccggc ggcgcgctct gacgacccca acaccaagat tctcggcgac
     1321 gacgagcgat gggctatctc gcccgggtac acggtgctgc cgctggcggg acccgagcgc
     1381 tacacgacag tgacgcgcgc gccctacccg gacggctcgc gttggtgggt ggtctcaatc
     1441 ctgacgctga aggacgatcg cgtggcccac gctgagacct atttcgcgcc ggagtttgag
     1501 ccgccggagt ggcggaagga catggtcgag gtcgtcaccc gggactgagg tcgcttttgc
     1561 tacccggacg gaccagctgg ttcgccgttc tgctcgtaaa ggtccgccgc gactctcgag
     1621 tgagcccact agacttcgag gcctagaggg agggctccat cgacgcatcg catcgcggaa
     1681 agacggttca tggcaagctg gcgaatgccg gacatcgggt gctcgcccca gacgatgcgg
     1741 aggtagaggc ctacctcgag gtcgcgctcg acgcaccggc accccaacgg ccagttctcg
     1801 accccccgac gcccgacaac gtgatcgtgc tcgacttcgg tggccagacg gcgcagctga
     1861 tcgcgcgtcg cgtccgcgag ctgaacgtct attcagagct gatgcctttt gacaccccgt
     1921 gggaagacat actccggagg cggccatcgg cagtgatcct ctcgggcggc ccgatgagcg
     1981 tctacgagga cggtgcaccg caccccgacc cccggatctg gggctccact gacatccccg
     2041 tcctgggcat ctgctacggc gttcacctga tggcgcacca cctcggcggc gaggtgatcc
     2101 ccgccggtaa acgcgagtac ggaccggcca ccgtgtcgat cacgaccgag gacagcctgt
     2161 tcaagggcct cgacaagcag caggccgtat ggatgagcca cggcgactcg atcgtgacgc
     2221 cgccgcccgg cttcacgccg ctggccgaga cggagtcgac gccctacgcc ggcctggccg
     2281 atacggcccg gcgcatgtac ggcatccagt tccatccgga ggtcgtccat acggcggctg
     2341 gacgtgacat cctgcgcaac tttgtgctcg ggattgccgg ggcgacgcct tcctggacgt
     2401 cggcctcgtt catcgagtcg acgatcgccg atatccgcgc ccgcgtcggc gacggcaaag
     2461 tcatctgtgc cctgtcgggt ggtgtcgact cggcggtcgc ggcaacgctg gtccatcggg
     2521 cggtgggcga tcagctgacg tgtatctacg tcgatcacgg gctcatgcgc aagcgcgaga
     2581 gcgagctcct gcgccagacg tttgaacaga acctgggcat gaacctggtc atggtcgacg
     2641 ccaccgagcg cttcctgcga aggctgatgg aggtctccga cccggagtca aagcggcgca
     2701 tcatcggcga cgagttcatc cgcgtcttcg aggaagaggc gaccaaactc gggcagatcg
     2761 atttcctgac ccaggggacg ctgtatcccg acgtgatcga gtcggccacg cccgaaacca
     2821 aggcagccca gaagatcaag acccaccaca acgtcggcgg gctgccgtcc gacctccggt
     2881 tccagctgat cgagccgctg cgctacctgt tcaaggatga agtgcggacg gttgggcttg
     2941 agttggggct gccagagacg atggtgcacc ggcagccgtt cccggggccg ggcctcgcga
     3001 tccggatcat cggcgaggtc acgaaggagc ggctcgacat cctgcgcgaa gccgactgga
     3061 tcgtgatcga cgagatcaag gccgcgggtc tctaccgctc gctgtggcag tcgttcgcga
     3121 tcctgacgcc tgtccggtcg gtcggcgtca tgggcgacgg tcgaacctac gccaacgttg
     3181 tcgccgtccg ggctgttacg tccgaggatg ggatgacggc cgactgggcc aagctgccgt
     3241 atgacgtcct ggccaagatc tcgtcgcgca tcgtgaacga ggtacccgac gtcaaccgcg
     3301 tcgtgtatga catcagcagc aagccgccgg ccacgatcga gtgggagtag gtagtccgta
     3361 gccgcctttc tcatacgggc gcagccttcg ccttcgagcc gcttcgattg gccatgttcc
     3421 gcgtctggcg ttagcccggg tgccgcatga ccagagttcc cgcgccgggc atctcgtaca
     3481 tcaaggaaga ggccgtcacc ctgcgtggac ccaagggtga gcccggggag cggctatccg
     3541 ccggcatgac caacccgagc gtcatggacg acggctggtg gctgacacac ctgtgggtcg
     3601 cggacggcga gggggtagtg gacgccgcaa cggttgcacc cgccgctggg ccgccaccgg
     3661 gcacgccact cgccactctg gggccgcgcg tcgccggatc gctttccggg ctgatcgccg
     3721 aggaggacgg ccgccagctg atccgcctcc gcatgccgga ggcacccgat gagtcgcgtc
     3781 cgtgggagcg gccgctgctg tgcatggtcg ctattcggtg ggatcccgtg cgcgcctcgg
     3841 tgatcacacg caaccagctc gcgcgtgagc tgctgcgcgg tttcgcggca gccgtggaag
     3901 ggctaggccg accgggctag gaagtcttct gcggcggggt tagcattcgg cggtggacgt
     3961 caccctcgcc cccgagcggc tcttcgtcct cgaagagcgc ctgaccgtcg acgagatcca
     4021 gcagcgtgcg atggacaagc gcacccaggc gttcggcggc ggcctcggcg ccttcctgca
     4081 acggccgaag accgacgaag tggcgctcgt cgccaagcag cgtcgcctcg agccgttctg
     4141 gcacgttcgc ggtaaggcca cgtacgtcta cgagcggaac cgcagctata ccgtgccggc
     4201 cggttccgca gacgtggagg ccgtgaccat caacgggacg cgctacgacg gcgcgcccgc
     4261 tccgcagcgg accttcgccg tccccgtcct ggaacattgc cgagccacgt tcacaagtga
     4321 gtcgttcacc gatggcctga ccggcgctgc ggtgccggaa ggatcggcgc tcatcacggg
     4381 tgtccgatca gaggtcggcg acccgcagac gctgacagcc aacgagacgc tcgccgagcc
     4441 acccgagcac cgtgcctcgt tcgtcgttcg tcaggtgatg agccagatca tgaagccggt
     4501 ccaggccgat cgcgtgctcg aggaggccgt cgagctcgag gtgaccgacc tctactaccg
     4561 cccatggtgg gcgttcgagt tcacgtggca ggggaagggt aagcagggtg tcgtcgagat
     4621 cgatgcgatc accggcaaca cgcgccaggg caagccgctc accggccaga tccgcagcat
     4681 gttcaaccgc gacgttttat tcgatgtcgg cgccgacact gtcggcctgt tcgttcccgg
     4741 gggcagcatc gcggtcaaga tcgcgaaggc cgcgatcgac tccaacaaga agtaggcgcg
     4801 ggggcacacc tggagacgtc gcgcgtcctc gtcgtccccg gccttcgcac gatcccgcac
     4861 agaggattcc gcgctcggct cgctaccgcc ttggcggtcg tcgctggcca gccacgactg
     4921 tggcttctgg gcgcagcggg tttcctgttg cgcggcggaa tcgtcgtcct gatcgccccc
     4981 gtcctggtcg tcccaacgca agtcgagatc cgcttgctgc tcggtgacta cctcggcacg
     5041 agcggaatca cgccatcgtt ctatcccctg gtgatcggca tagcggtggt cgccgcggtg
     5101 gttctcctcg ggctcgtcct cgtcctggct cggctggagc tggggctttt cgacgccctc
     5161 agcactgacg aacgagtcgc cgcacacgtc ggcgactggc agccgattgc gcgcggcgag
     5221 gcatccagag gcactctctt cgcacgcctc gtcactgtcc aggtatggac ggtgatcgcg
     5281 atcctcatcg cggcattgcc cctggcactg gcgatcggcg aaacagccta tgccgaggtg
     5341 atccggccca cttcccccgc gcccatctac cagcgcgtac tgggtgcggt gaccgtgccg
     5401 ttcatgctgt tcgtaggcgc gttcctggcg attgaggcgc tgtcggcagt tatctcgcgc
     5461 cgtctgttgg tcggccgccg cagctccctc gggctcatcg gcgcggcaac gtcaggtctc
     5521 cgccgtgtga tcaggtcgcc gttgcgaacg ctcgcggccg cgctctcagg ctgggcgctc
     5581 gccctcgttg ccctgacacc agttctggcc atcatcgtcg tgacgtggca acccgttcgg
     5641 cgcgcctttc tctcttcggt ctcttttggg gacgttgtcg aaccacgcac cctcatggcc
     5701 gcgatcgtgc tggccggtag cttcgttatc gggctcgttg tggccggctt cgtgtcggcc
     5761 gttcggtcgg ccatctggac cgtcgatagc gtctcctgag aggacggccg accggcccgg
     5821 cagggcatgc cgcatcactt gtggcgcgct cgtaacgtca attcgggagc gtgggcgtgc
     5881 ctcgcacacg ggggacgctg ataaactcac gccgcccgcg gccgtcccct cactggccgc
     5941 ctacgtgagg caactgcccg aatgattgac cagctcctcg acacgctcgg gagcgaaatt
     6001 ggcaaaatct tcgactcgcc gctggtgcgg ctgtcgctcc aggcgatcgg cttttacgtt
     6061 gtcctgctgt gggcggcgac ggcttactgg gcgtatcgcg atcttcagac gcgcaccacg
     6121 aaccccgtcg cgccgtacct agcggcggcg ctgatcatcc tgttcacgcc ggtgttcttc
     6181 ttgttcgggg tgatcgtcta ccgcatcctc cgaccgtcgg agaccgtcgc cgaggccaac
     6241 gaaagggcac tcgccgaaga ggcgatgatg gtcgagatcg agtcccagcc gcactgcgcg
     6301 aactgttcgc gacaggtgca cgaggactgg atcatctgcc ccacgtgccg caaccggctc
     6361 cgccgcgtgt gccccaactg ctcacgcttg gtggagctcg attggtccct ctgcgcctgg
     6421 tgcggcaagg acttcgagcg cccggagctg cttcaggatg tcgtcctgcc ggttccgtcg
     6481 ggcaagcagg cgcgccaggc ggctgacacc tacatcgacc agccgaccga tacgcagttc
     6541 gagccgccgg ctcccgtgcc gcagatcgcc tcgagcgcag ccgaaaaccg ggtccctgag
     6601 cgagcgccgc gggccgctcc gaccgagaac tgacgtccga aatttccatc gagcgcccgg
     6661 acgccggccc cgctgccggc cctgctgatt caccgggtag cttctccgta gagggccggc
     6721 ccgctgccgg tctctatctt ctcgcctggc ttttgggggt cggtggcttc gtcgtgttgc
     6781 tgatcgccgc cggcgcgcct ccgccgtggg gcggtctgtt gttcatggca gcgctgctgg
     6841 ctacgacgct cggactcgga gcggcagcgg gctaccaact gatcgccagg gtcacgcgag
     6901 ctgcctcggc attccgtggc ccgtcaccgg ccatcctgct cgccctccag ctcatcgtgt
     6961 cgatcgcact gggaacggca tgggtcgttc tcggagtccc agatccgcag tccagctccg
     7021 ttgggttttt gatcattgcc gcgacgctgc tcgtgacgta tgtgggcatc gtctggctct
     7081 tcgccgttcg ctccggcgtg ctttcgtgga gcgaaatggg ctggccgacc cgtgcgcctg
     7141 cctcacggct gctgggcgac atcggcgttg ggttcgcggc aatgctggtg gcctggcccg
     7201 ttgtcacctt cctggccgcg ctgctcggga ccttgctcca gtcgacgccg accaaccccg
     7261 ttccatcgat agcgacgacg accgacacca tcttgaccgc gctcggggcc gcgctgctgg
     7321 tgccaatcgg cgaggaagtg ctgttccggg gctacgcact gacggcgtgg ctgcgcgatc
     7381 tgggaccgcg gactgcgctg attcgcagca ccatcttttt cgccttcgcc cacatcctcg
     7441 gcgtgactgc cgcctcgttc gacgagggtt ggcgacaggc cctgttgacg gtcgtggtga
     7501 taactccggt cggcgccctc ctgggagtcc tatttctgcg ccggggtttg cttgcggcga
     7561 tagccggaca cgtcacgttc aacctcgtca gcgtgctcgc gatggcactc gcccagtccc
     7621 ttccgccgcc ccaatagaga ctgcggacag agcagaactg tccggtgagc cagtgttgat
     7681 cggcgggcta cggcgggtga agaacgggtt cctagagttc acgtcgctcc ctcgttcgga
     7741 tggcggggaa atcggcgggc tccgttgtcc ggacctggga gcgtttcacc
//
LOCUS       DATLCO010000004         4660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9691671, whole genome shotgun sequence.
ACCESSION   DATLCO010000004 DATLCO010000000
VERSION     DATLCO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9691671"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VMZ33_00140"
     CDS             <1..209
                     /locus_tag="VMZ33_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15665.1"
                     /translation="WSAMGAVSPLLSLRDQKPLHAVPVLVFVVALSLLVLSVVALVMS
                     GAFQPEAHHLVAPFRWFYGDSATA"
     gene            245..805
                     /locus_tag="VMZ33_00145"
     CDS             245..805
                     /locus_tag="VMZ33_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15666.1"
                     /translation="MPTVEPRTAPATRSRRPSRAPVLLVIIGFGLATTLVLIGALLAS
                     LPAPKPSIDQPGTVESPRSLNVIMRDYRFDPTPIYVVRGETIRLTVLNGGLIEHELVL
                     GDAQVQSAWAAADAAATPPAPFATAPPASVSPDVGGLRVVLASGASQVVDYTVPTSSA
                     EPIQMVCHLPGHVARGMIGAVELSAR"
     gene            849..1139
                     /locus_tag="VMZ33_00150"
     CDS             849..1139
                     /locus_tag="VMZ33_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HUR15667.1"
                     /translation="MTYVIAEPCIDVLDESCVAVCPVDCIHFDRGPDRKLYIDPDECI
                     DCGACEPECPVNAIFPEDSVPAEWAQYTAIDATWFKDKDAARQMVDELKPVA"
     gene            1198..2136
                     /locus_tag="VMZ33_00155"
     CDS             1198..2136
                     /locus_tag="VMZ33_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HUR15668.1"
                     /translation="MRIVSLLPSATEIVAALGLGDELVGRTHECDFPLDVQSVPVVTS
                     DVGDTVNASSHEINARVAASIHGGSSIYKLDMAALAAAEPDLILTQELCTVCAVSYRD
                     VTEAVRQLDSDATVISLEPTSIEGIFNTISTVGAMTEAEDEAIGLIELLRERLATVEN
                     RVLERRLQGIPPRRVVCLEWLDPPFASGHWVPEQARRAGGWDVLGRQGEPAIETSWDS
                     IREVDPEQIMLIPCGFDARRTAHEWQQTRDARPKWFDDLKAVREGEIFALDGSAYFSR
                     PGPRIVDGVALLADLMDPEFADETLPLDAWLPVEVG"
     gene            2235..3011
                     /locus_tag="VMZ33_00160"
     CDS             2235..3011
                     /locus_tag="VMZ33_00160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR15669.1"
                     /translation="MGKAQDNDFLRYRLREGLRKRSEAERAAGKSDDESLRGYYRARA
                     PEYDDWYLRRGRYSHGVIADAAWNAELDTATLWLDAMPLHGEIVELAAGTGWWSPLLA
                     QKADHGGLWLYDAVEETLDKARDRLVAHGLAAHIHVRDAWAEPDRPVDGLFAGFWLSH
                     VPRARIGDFLALCRRWLKPTGTFVFIDSRSDPESGASDHPAPADDRSTRRTTDGREFT
                     IPKVYWEPAELQSALHDAGFTSAEVTTTPRFFLLGRALAA"
     gene            3034..3876
                     /gene="proC"
                     /locus_tag="VMZ33_00165"
     CDS             3034..3876
                     /gene="proC"
                     /locus_tag="VMZ33_00165"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HUR15670.1"
                     /translation="MSLRDSVIATVGSGVMAEAMIAGLLRGEQVAPEQIIASHPRPDR
                     RTELVSSYGIRTVEDNASAVAEADVIVLALKPQMLARVGRELRPAMTDGKLVISVIAG
                     ATTNAIANVLHHRQIVRSMPNTPAQLGRGMTVWYGTPEATEDQRAQARVLLATLGMQL
                     EVDDEKLVAMATAVSGTGPTYVFLVMEALIDAAVHLGFPRHMAHDLVVETLEGSALFA
                     KSSNMHPAQLRNMVTSPGGTSAAALHELESGRLRTVLSEAVWAAFRRTEELGKILESD
                     TAKD"
     gene            complement(3894..4265)
                     /locus_tag="VMZ33_00170"
     CDS             complement(3894..4265)
                     /locus_tag="VMZ33_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15671.1"
                     /translation="MMRNAVEWAVLAFSVLAIATLAGALVLTGLNEQRPAEPLVELRA
                     AEARTTALGWLIPATVRNAGDETAEAVVLEAAAQVDGKEEISEMEVAFLPAGSESEIV
                     FAFSAEPESEVTVRLVGFGLP"
     gene            complement(4262..>4660)
                     /locus_tag="VMZ33_00175"
     CDS             complement(4262..>4660)
                     /locus_tag="VMZ33_00175"
                     /inference="COORDINATES: protein motif:HMM:NF021161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2391 family protein"
                     /protein_id="HUR15672.1"
                     /translation="LLVAAGGALIFALNVAPTEEPMMLGIEAPYWLLVVVIGASLALT
                     YALVFFADFGGKRRFGEGTLGNPLSETVAAYAVSLVVSVLLLWSFGRLDGTGFAPILG
                     MTIMLTVVASVGAAVARLLIGGHSEAGASP"
BASE COUNT          793 a         1620 c         1504 g          743 t
ORIGIN      
        1 aatggagtgc catgggcgcc gtatcgccgc ttctctccct ccgcgaccaa aagccgctac
       61 acgcggtgcc ggtcctcgtt ttcgtcgtcg cgttgagcct cctcgtgctg tccgtcgtcg
      121 cgctcgtgat gagcggcgct tttcagcctg aggcgcacca tctagtcgcg ccgttccgtt
      181 ggttctacgg ggactcagct acggcgtaga acgctcgccg ccgtggaccg cataaagtca
      241 ggcggtgccc accgtcgagc cacggaccgc cccagcaaca cgcagccggc ggccgtcgcg
      301 cgcgccggtc ctgctggtga tcatcggttt cggcctggcc acaacgctgg tgctcatcgg
      361 cgcactgctc gcgtccctgc cggcgcccaa gccgtcgatc gaccagccgg gaaccgtcga
      421 atccccgcgg tcactcaacg tgatcatgcg cgactaccgc ttcgacccga ctccgatcta
      481 cgtggtccgc ggcgagacca ttcgcctgac ggtgctgaac ggcgggctca tcgagcacga
      541 gttggtcctg ggcgacgcgc aggtgcagtc cgcgtgggcc gccgcggatg ccgccgcaac
      601 gccgcccgcc ccgttcgcga cggcgccacc ggcgagcgtc agcccggatg tcggcgggct
      661 gcgcgtcgtg ctcgcctccg gtgcgtcgca ggtcgtcgac tacaccgtgc ccacgagcag
      721 cgcagagccg atccagatgg tctgccatct tcccggccac gttgcgcgag gaatgatcgg
      781 tgcggtcgag ctgtctgccc gttgagcgtg cggcccaccg ctcagacaat cgctaacatg
      841 gcgcgaccat gacctacgta attgccgaac cgtgcatcga cgtcctcgac gaatcgtgcg
      901 tcgcggtgtg ccccgtggac tgcatccact ttgaccgcgg ccccgaccga aagctttaca
      961 tcgacccgga cgagtgcatc gactgcggcg cgtgcgagcc ggagtgcccg gtcaacgcga
     1021 tcttccccga ggactccgtc cccgccgagt gggcgcagta caccgccatc gacgctacct
     1081 ggttcaagga caaggacgcc gcgcgccaga tggtggacga gctcaaaccg gtcgcctgac
     1141 cgcaggacct gaccgcgcgc ccggcccagc tgggctgact cgcggacacc aacaacgatg
     1201 cggatcgtct cgctcctccc ttctgcgaca gagatcgtcg ctgccctcgg cctgggcgac
     1261 gagctcgttg gccgtacaca cgagtgcgac ttcccgcttg acgtccagtc ggtgccggtt
     1321 gtcacttctg acgtcggcga caccgtcaac gcaagcagcc acgagatcaa cgctcgtgtc
     1381 gcggcttcga tccacggcgg cagctccatc tacaagctcg acatggcggc gcttgcggcg
     1441 gccgagcccg atctgatcct cacccaggag ctttgcaccg tctgcgcagt cagctaccgc
     1501 gatgtcacgg aagcagtgcg ccagctcgac tctgatgcca cggtcatcag ccttgagccg
     1561 acgtcgatcg agggcatctt caacacgata tcgacggttg gcgctatgac tgaggccgag
     1621 gacgaggcga tcggcctgat cgagcttctg cgcgaacgac tggccacggt cgagaaccgc
     1681 gtccttgagc gccggctgca gggcattccg ccacgccggg tggtgtgcct cgagtggctc
     1741 gacccgccct tcgcctctgg ccactgggtg cccgagcagg cccgtcgcgc cggcggctgg
     1801 gatgtccttg gccgtcaggg cgagccggcg atcgaaacgt cgtgggacag cattcgggag
     1861 gtcgaccctg agcagatcat gctcataccg tgcggcttcg atgcgcgccg caccgcgcac
     1921 gagtggcagc agacgcgcga cgcgcggccg aagtggttcg acgacctgaa agccgtgcgt
     1981 gaaggcgaga tttttgcgct cgacggatcg gcgtacttca gcaggcccgg gccacgcatc
     2041 gtcgacggtg ttgcgctcct tgccgacctc atggatcccg agttcgccga cgagaccctg
     2101 ccgctcgacg cctggctccc ggtggaggtc ggctgatctt ccagccgcaa ttcaactgcc
     2161 tgtggtgcgg tcgaccgcac actacacgcg ggccgaccga tctcgagggc tgggcgcgcc
     2221 tgtgctccga ttgcgtgggc aaagcccagg acaacgactt cctgcgctat cggctccggg
     2281 aggggcttcg caagcggtcg gaggcggagc gcgcggccgg caagtcggac gatgagtcgc
     2341 tgcgtggcta ctaccgcgcc cgagctcccg agtacgacga ctggtatctg cggcgcggca
     2401 ggtacagcca cggcgtgatc gccgatgctg cctggaatgc cgaacttgac accgcgacgt
     2461 tgtggctcga cgcgatgcca ctccacggcg agatcgtcga gctcgcggct ggaacaggct
     2521 ggtggtcgcc gctcctcgcc caaaaggccg atcacggcgg gctttggctc tacgacgcgg
     2581 tagaggaaac gctcgacaag gcgcgcgacc gactcgttgc ccatggcctc gctgcgcaca
     2641 tccacgtccg cgatgcgtgg gccgagcccg accgacccgt cgatggcctg ttcgccggct
     2701 tctggctgag ccacgtcccg cgcgcgcgca tcggcgactt tctggcgctc tgccgccgct
     2761 ggctcaaacc gacaggcacc ttcgtattca tcgattcacg gagcgaccct gagtcgggag
     2821 catccgacca cccggcaccg gccgacgacc gatcgacccg ccgaaccaca gacggccgcg
     2881 aattcaccat cccgaaggtc tattgggagc cggcggaact gcaatcggct ctccacgacg
     2941 ccgggttcac gtcggcggaa gtgacgacaa ccccaagatt cttcctgttg gggagagctc
     3001 tggcggccta gggcgcacct atactcgccg ccaatgtcgc tcagggatag cgtgatcgcc
     3061 actgtgggct cgggtgtgat ggctgaggcc atgattgccg ggctgcttcg tggcgagcag
     3121 gtcgcaccgg aacagatcat cgccagccac ccgcgacccg atcgtcgtac ggagctcgtc
     3181 agcagctacg ggatcaggac cgtggaggac aacgccagcg ctgtggcgga ggccgatgtg
     3241 atcgtcctgg cgctgaagcc gcagatgctg gcccgggtgg ggcgcgagct gcgcccggcg
     3301 atgaccgacg gcaagctcgt catcagcgtc attgccgggg ccacgacgaa cgccatcgcc
     3361 aacgtcctcc atcatcgcca gatcgtgcgc agcatgccca atacgcccgc tcagcttggg
     3421 cgcggcatga cagtctggta cggcacgccc gaggctacgg aggaccagcg cgcccaggca
     3481 cgcgtactgc ttgcgaccct ggggatgcag ctcgaggtcg acgacgagaa gctcgtggcc
     3541 atggccacgg ccgtcagcgg gacaggtccg acctacgtat tcctggtcat ggaagccctg
     3601 attgacgcgg cggtccacct cgggttcccg cgccacatgg cacacgacct cgtggttgag
     3661 acgctcgagg gcagcgcgct tttcgccaag tcgagcaaca tgcacccggc ccagctgcgg
     3721 aatatggtca cgtcgcccgg cggcaccagc gccgcagccc tgcatgagct ggagtcgggc
     3781 cggctccgca ccgtattgag cgaggcagtt tgggcggcct tccgccgcac cgaggagctg
     3841 ggcaagatcc tcgagtccga caccgccaag gactagctgc gcccgaaggc cgactagggc
     3901 agcccgaagc ccaccagtcg gaccgttacc tccgactcag gctcagcgct gaacgcgaat
     3961 acgatctccg actcggaccc cgccggcagg aatgccactt ccatttcgct gatttcctct
     4021 ttgccgtcga cctgggcggc agcctccaat acgaccgcct cggcagtctc atcgcctgcg
     4081 ttgcgaacgg tggcaggtat cagccagccg agggcggtcg ttcgcgcctc ggcggcccgc
     4141 aactcgacca gcggttcggc cggacgctgc tcattcaggc cggtgagcac caatgcccca
     4201 gcgagcgtgg cgatggccag gacgctgaag gcaaggactg cccactcgac cgcgttgcgc
     4261 atcatgggct agcacccgcc tcactgtggc cgccgatgag cagcctagcc accgccgcgc
     4321 cgacggaggc gacgacggtg agcatgatgg tcatgcccag tatcggcgcg aaacccgtgc
     4381 cgtcgagccg gccgaaggac cacagcagaa gcacagagac gaccaggctg acggcgtacg
     4441 cggcgacggt ttccgaaagc ggattaccga gagtgccctc gccgaaccgc cgtttgccgc
     4501 cgaagtcggc gaaaaagaca agcgcataag tcaacgccag gctggcccct atgaccacga
     4561 ccagcagcca gtagggcgcc tcgatgccga gcatcatcgg ctcctcggtc ggcgccacat
     4621 tcagagcgaa gatcagggca cccccggccg cgaccagcag
//
LOCUS       DATLCO010000005         5116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1496516, whole genome shotgun sequence.
ACCESSION   DATLCO010000005 DATLCO010000000
VERSION     DATLCO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496516"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VMZ33_00180"
     CDS             <1..945
                     /locus_tag="VMZ33_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15673.1"
                     /translation="SHISVFSLFQLPLGAFRVNDLIFVYEERGQIAGLARVERDGLRD
                     EWTIVELDAVDNGEAGDIRFRLVQHLLRDASKRGALRFHVACADTGGNVDLFMQAGFA
                     RYGEERILFRPPAPETSHSPTITATQAAVRGIRPAGPLDAIELDRLYHAATPAPVARL
                     EDYRLHDWERQGVHWRVPRSSLTPLLRFADVEAFVQQSAEATSPELLAFCQIGVAKQE
                     QPHYIRVITRPEHDPSDLIDYGLGVIADRRRVGLVDRAVRAHPSERGVISAVRTYESP
                     LDRRLSDQGFADLSTVSLLMKEVAVRVFEPSLVAAVTN"
     gene            955..3057
                     /locus_tag="VMZ33_00185"
     CDS             955..3057
                     /locus_tag="VMZ33_00185"
                     /inference="COORDINATES: protein motif:HMM:NF040318.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HUR15674.1"
                     /translation="MTALKADMNDDSGVNENTVEARSDRADDLEALLIAFPPEIANRL
                     RSIPAERDMIEIVMDLGRRPEARFGGGGEEVLLERDVTQEDIQYVVDHIGSFGDDNRA
                     GIERTLHRISAIRNRSGKIVGLTARIGRAVFGTIEIIKDLVESGKSVLIMGRPGIGKT
                     TMLREAARVLADEIDKRVVVVDTSNEIAGDGDIPHPGIGRARRMQVRTPEQQHGVMIE
                     AVENHMPQVIVIDEIGTELEASAARTIAERGVQLIGTAHGNNLDNLMLNPTLSDLIGG
                     IQSVTLGDEEARRRRSQKSVLERKAPPTFDVVVEIQSRDRVTVHADVSDTVDAMLRGD
                     PVAPELRYRDEGGVHRSQARPRPGPQAQIPGERFGGLVASGGGASSFGGWRTEPGWRP
                     DARRGEAAAQREPGREAERGYRPGYHPGATGGWRRGSTGGAGGGGPFLQGEVADSGPL
                     ERGTPEPEHRARDIREYERQREWRESASRAITQLRAGETSPDNEDADVENEADELQDD
                     FLVADYSLIEEELSGDRRPTQLPASGSTSLPTLKALPHGVSNKKLEQAIRELDLPVTI
                     VRDADEADVVITLRNHFKQKAPALREAEERGLPIFVLKSNTLIQLESALTSIFALEVD
                     PRDAALREVEEAIGLVHDHSKPVELSPQNSYIRRMQHQMAERANLVSQSRGREPYRRV
                     RLYPDSEAKPAGRLARRS"
     gene            3169..3765
                     /locus_tag="VMZ33_00190"
     CDS             3169..3765
                     /locus_tag="VMZ33_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUR15675.1"
                     /translation="MVSGTQGDLAAGNVTVSAESDEELIERLAATADEVALSELYDRY
                     QAPMYGLAMRITRDAALAQDAVQEAFVGVWRNADRYAAGRASVRTWILSITHHRAIDV
                     LRRRRPTTTLPDTETVDEALRSPDVWPEVVRALDGDAVRTAIDGLPQIQRQAIEMAYF
                     EGLTQVEIAERTGAPLGTVKSRVRLGLQTMRRSLEGVT"
     gene            3774..4634
                     /locus_tag="VMZ33_00195"
     CDS             3774..4634
                     /locus_tag="VMZ33_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HUR15676.1"
                     /translation="MTHSEAIERSGLYVLGALEPSEKRAVDEHLATCPELHAEYAELG
                     GVVPALANLAAPMDAPAGLKSRVLAAVAREAESARVAASVGASSSFAAPTTPAIAPPP
                     TDDSAVKPWELDVDQAPSLAARAAPRGWMGWAAAAMAVALLAVVGVWGLGAQSRADAE
                     GRRAQELAAALQVFGAPDAEVATLRGTGAAAGANGFAAFSPAGDGYVVVSGLATLSSD
                     QTYQAWYLVGDQPFSAGLMSVGDDGMAMLARIPHHSGTNVIALTIEPAGGVAAPTSAP
                     VVAGELRRSA"
     gene            4642..>5116
                     /gene="tmk"
                     /locus_tag="VMZ33_00200"
     CDS             4642..>5116
                     /gene="tmk"
                     /locus_tag="VMZ33_00200"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HUR15677.1"
                     /translation="MSVFITLEGPDGAGKSTQLELLAQRIRSLGREVVTTREPGGTEL
                     GERIRHLVLGTSGDHDGLVDALLFNAARRQLVREVIQPGLARGAVVLCDRFADSTLAY
                     QGFGSGAPLEDLILIAEAATSGLTPTRTVLFDVDVSAGLARRLGGDHEALTKFETS"
BASE COUNT          864 a         1701 c         1746 g          805 t
ORIGIN      
        1 agtcacatca gcgtgttcag cctcttccag ctgccactcg gcgcatttcg ggtcaacgac
       61 ctgatcttcg tctacgagga gcgcgggcag atcgcgggtc tggcccgggt ggagcgcgac
      121 gggctcaggg acgaatggac gatcgtcgag ctcgacgccg tcgacaacgg cgaggccggc
      181 gacatccgct tccgcctggt ccagcacctc ctcagggacg ccagcaagcg cggcgcgctg
      241 cgtttccacg ttgcgtgtgc cgacacgggc ggtaacgtcg acctcttcat gcaggctggc
      301 ttcgcgcgct acggcgagga gcggatcctc ttccgtccgc cagcgccgga gacgagccat
      361 agcccgacta tcaccgcgac gcaggccgca gtccgtggca tcaggccggc cggcccgctc
      421 gacgcgatcg agctcgatcg gctttaccac gccgcgacgc cggcccctgt cgcgcgattg
      481 gaggactatc gcctccacga ctgggagcgg cagggtgtcc attggcgcgt cccgcgctcg
      541 agcctgacgc cgctcctgcg ctttgccgac gtcgaggcgt tcgtccagca gtccgcggag
      601 gcaacgagcc cggagctcct cgcgttttgt cagatcggcg tcgcgaagca ggagcagccg
      661 cactacattc gggtcatcac gagaccggaa cacgatccct cggacctcat cgactacggg
      721 ctgggcgtga ttgccgaccg ccgacgcgtc gggctcgtcg accgcgccgt gcgcgcccac
      781 ccatcggagc gaggcgtcat ctccgccgtc cggacctacg aatctcccct ggatcggcgt
      841 ttgtccgacc agggctttgc cgatctttcg accgtctccc tgctcatgaa ggaggttgcc
      901 gttcgggtgt tcgaaccatc tcttgtcgca gcggtcacga actaggggac gtccttgaca
      961 gcactgaaag ccgacatgaa cgacgattca ggcgtgaacg agaacaccgt ggaggcacgc
     1021 tctgatcgcg cggacgacct cgaggcgctg ctgatcgcct ttccgcctga gatcgccaat
     1081 cgccttcgca gcatcccggc agagcgcgac atgatcgaga tcgtaatgga cctcggccgt
     1141 cggcccgagg cgcgtttcgg tggtggcggc gaggaggtcc tgctcgaacg cgacgtgacg
     1201 caggaggaca tccaatacgt cgtcgaccac attgggtcct tcggggacga caaccgcgcg
     1261 ggcatcgagc gcaccttgca tcgcatctcg gcgatcagga atcgcagcgg caagatcgtc
     1321 ggtctcacag cgcgcatcgg ccgcgccgtt ttcggcacca tcgagatcat caaggacctc
     1381 gttgagtcgg ggaagagcgt tctgatcatg ggtcgcccgg ggatcggcaa gacgacgatg
     1441 ctgcgcgagg cggcgcgcgt cctggctgat gagatcgaca agcgcgtcgt ggtcgtcgac
     1501 acgtccaatg agatcgccgg cgacggggac atcccgcatc cggggatcgg tcgcgcacga
     1561 cgcatgcagg tacgcacgcc cgagcagcag cacggggtga tgatcgaggc ggtggagaac
     1621 cacatgcccc aggtgatcgt catcgacgag atcgggacgg agctcgaggc cagcgctgcg
     1681 aggaccatcg ctgagcgtgg cgtccagctg atcggcaccg cccatggcaa caacctcgac
     1741 aacctgatgc tcaacccgac gctgtccgac ctgatcggcg gcatccagag cgtcacgctc
     1801 ggcgatgagg aggcccgccg acggcggagc cagaagagcg tgctcgagcg caaggcgcct
     1861 ccgaccttcg acgtggtcgt tgagatccag tcgcgcgatc gcgtgacggt ccacgctgac
     1921 gtttccgaca cggtcgacgc gatgctccgc ggcgatcccg tcgccccgga gctgcgctac
     1981 cgcgacgagg gtggtgttca ccgctcacag gcacgtccgc ggccggggcc gcaggcacag
     2041 atcccgggcg agcgcttcgg tggcctggtg gcgagcggcg gcggcgcgag ctcgttcggg
     2101 ggttggcgca ccgagcccgg ctggcgtccc gatgcgcggc gtggggaagc ggccgctcaa
     2161 cgagagcccg ggcgcgaggc cgagcgtggg tatcggcctg gttaccaccc gggtgccacc
     2221 ggcgggtggc gccgcggcag cacgggaggc gccggaggcg gcggcccttt tctgcaaggc
     2281 gaggtggccg atagcggtcc cctcgagcgg ggcacgcccg aacccgagca ccgcgcgcgc
     2341 gacatccgtg agtacgaacg ccagcgcgag tggcgcgagt cggcgagccg ggcgatcacc
     2401 cagctgcgcg ccggagagac gtcgcctgac aacgaggacg cggatgtcga aaacgaggca
     2461 gacgagctgc aggacgactt cctggtcgcc gattacagcc tgatcgagga ggagctgtcg
     2521 ggcgatcgcc gaccaacgca gctgcccgcg agcggcagca ccagcctgcc gacactcaag
     2581 gccctgccac acggcgtcag caacaagaag ctggaacagg cgatacgcga gctggacctg
     2641 cccgtgacga tcgttcgcga tgcggatgag gccgacgtcg tgatcacgct gcgcaaccac
     2701 ttcaagcaga aggcgccagc actgcgcgaa gcagaggagc gcggcctgcc gatattcgtc
     2761 ctcaagtcga atacgttgat ccagctggag tcggccctga cgtccatctt cgccctggag
     2821 gtcgacccgc gcgacgctgc cctgcgcgag gttgaggagg caatcggcct ggtgcacgac
     2881 cactcgaagc cagtcgagct gtcgccacag aactcgtata tccgcaggat gcaacaccag
     2941 atggccgagc gcgccaacct ggtctcgcag tcgcgcggcc gggagccgta ccgacgggtg
     3001 cggctgtatc cggatagcga agccaagccc gccggcaggc tggcacgacg tagctagtcg
     3061 tcccgtcacg gcgttcctcc cagcgaagga ccgtcgctag actttctggg tgcccgtttt
     3121 gtgcgtgccc cgaaaaagtg aaccgttgtg ccctagcgga cgaacaacat ggtgagtggc
     3181 acgcagggag acctggccgc tggcaacgtg accgtgtccg ccgaatccga cgaggagctc
     3241 atcgagcgtc tcgcggcgac ggctgacgag gtcgcgctgt ctgagctata cgaccgttac
     3301 caggcaccca tgtacggact cgccatgcgc ataacacgag acgcggcact cgcccaggac
     3361 gccgttcagg aggcgttcgt aggcgtttgg cgcaacgccg atagatacgc ggcggggcgt
     3421 gcttcggtgc gcacgtggat tctctccatc acccaccacc gtgcgatcga cgtgctgcgc
     3481 cgccgccggc ccaccacgac gctccccgac acggagacgg tcgacgaggc attgcgctcg
     3541 ccggacgtct ggccggaagt ggttcgagcg ctcgatggcg atgccgttcg gaccgcgatc
     3601 gacgggctgc cccaaatcca gcgccaggcc attgagatgg cctattttga gggtctgaca
     3661 caggtcgaga tcgctgagcg cacgggtgcg cccctcggga ccgtaaagag ccgcgtgaga
     3721 ctgggacttc aaacgatgcg acgttcactc gagggggtga catgaaccag caaatgaccc
     3781 atagcgaagc gatcgagcgc agcggtctgt acgtcctggg cgctctcgag ccgtcagaaa
     3841 agcgcgcagt cgatgagcac ctggctacgt gccccgagct ccatgctgag tacgccgagc
     3901 tcggcggcgt cgtgccggcc ctcgccaacc ttgccgcgcc gatggacgcc ccagcaggcc
     3961 tgaagtcgcg cgtccttgcc gccgtcgctc gggaggctga gagcgccaga gtcgctgcct
     4021 ccgttggtgc ctcgtcatca ttcgcggcac cgactacgcc ggccatcgca cccccgccaa
     4081 ccgatgactc agcggtgaag ccgtgggaac tcgacgtcga ccaggcgccc tccttggcgg
     4141 cccgggccgc gccccgtggc tggatgggtt gggccgcggc ggcaatggcc gtggcgctgc
     4201 tcgccgttgt cggcgtgtgg ggtctcggcg cgcagtcacg tgccgacgcc gagggccgtc
     4261 gcgcccagga gctggccgcg gcactccagg tgttcggtgc acctgacgca gaggtggcca
     4321 cgctacgcgg aaccggcgcc gccgcgggtg ccaatggctt cgcggcgttc tcacctgcgg
     4381 gcgatggcta tgtcgtcgtc agtggcctcg cgaccttgtc gtctgaccag acgtatcaag
     4441 cctggtacct ggtcggtgac cagccgttct cagctggact gatgtcggtc ggcgacgatg
     4501 gcatggccat gctggcacgc attccccacc attccggaac gaacgtgatc gctctcacca
     4561 tcgagcctgc cgggggcgtt gccgcgccga cgagtgcgcc cgttgtggcg ggtgagctcc
     4621 gccgctcggc ctaggggagc cttgtcggtc ttcatcaccc tcgagggacc cgacggcgcc
     4681 ggcaagagca cgcagctcga gctgctggcg caacgcatcc gctccctggg aagggaggtc
     4741 gtgaccaccc gcgaacccgg cggcacagag ctgggcgagc ggatccggca cctggtactg
     4801 ggcacgagcg gcgatcacga cggtctcgtc gatgcgcttc tgttcaacgc cgcacgacgg
     4861 cagctagtgc gcgaggtcat ccagcccggc ctggctcgcg gcgctgtcgt gctatgcgac
     4921 cgtttcgccg attccacact tgcgtaccag ggctttgggt ctggcgcgcc actcgaggat
     4981 ttgattttga tcgccgaagc tgcgacgagc gggttgacgc cgaccaggac ggtgcttttt
     5041 gacgtcgacg tgtccgccgg gctcgcgcgc cgcctgggtg gcgatcacga ggcgctgacg
     5101 aagtttgaga cgagca
//
LOCUS       DATLCO010000006         2398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10437600, whole genome shotgun sequence.
ACCESSION   DATLCO010000006 DATLCO010000000
VERSION     DATLCO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10437600"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1305
                     /locus_tag="VMZ33_00205"
     CDS             238..1305
                     /locus_tag="VMZ33_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HUR15678.1"
                     /translation="MTTIAYPRGSVYVGVDTHSDVHVAVAKDELGRHFGELAVPSSGR
                     GHRDLQAWARSFGHPEAWGVEGTGSYGAALARFLTAAGENVVEVNRPDRRARRFKGKN
                     DTIDAEAAARAVLSGEATIVAKTGDGVVEQIRVVRVARCGAMRARTQAANALKSLVVT
                     APDQLRDQLRSLTTIRLVRAAARLRPGADRDVTAATKLAMRSLARRYESLEEEIKILD
                     EELQQLTTQAAPGLVEMFGVGTDTAGALLVAAGDNPQRLRSEAAFAKLCGVAPIEASS
                     GKTARHRLNRGGNRTANAALYRIVIVRLGLHQPTKDYAAKRTAEGKSKKEIIRCLKRH
                     LVREVFAAITKQPAIEAGLAA"
     gene            1632..1823
                     /locus_tag="VMZ33_00210"
     CDS             1632..1823
                     /locus_tag="VMZ33_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15679.1"
                     /translation="MSEHQRDTTPPPAGALTIDELARLQNAEETRDAKELAADIWESD
                     AELDAFLADLRSSRDASLA"
     gene            1827..2234
                     /locus_tag="VMZ33_00215"
     CDS             1827..2234
                     /locus_tag="VMZ33_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUR15680.1"
                     /translation="MADVVIDTDVASVLQKDRAPAWVGRHIAGKRVWLSFVAVGELWK
                     WAEVRSWGAKTRSSLDDWIAARPIVPYDTEVARSWARLSAGAQRRGRPTPQNDTWVAA
                     CCVRHNLPLLTLNKKDFEGFARHDGLVLLGEDD"
BASE COUNT          460 a          792 c          782 g          364 t
ORIGIN      
        1 actcgtccac tgcgatgatg ccggtggcgg tcacgacgcg gatctgaccc tctctgtcca
       61 ctggctcttc ggatgccttt acgtctgatc tgtcggtccg cgtcgtggct gtcgagggcg
      121 cgtccaaccg agcgacagtg acctaatagg agcctggtga agaggccccg tcgcagtgct
      181 gtccggtcgc ccggtcggtg cgcgccatct atcccactag cgaaatagga gatggcaatg
      241 accacgatcg catacccacg cgggtcggtc tatgtcgggg tcgacacgca cagtgacgtg
      301 cacgtcgctg tggccaaaga cgagctcggt cgtcacttcg gtgaactggc tgtgccttcg
      361 tctgggcgcg ggcaccgcga cctgcaggcg tgggcccgaa gcttcgggca ccccgaagcc
      421 tggggcgtgg aaggcaccgg ttcttatggt gcggcgctgg cgcggttcct caccgccgcc
      481 ggcgagaacg tcgtcgaggt caaccggcct gaccggcgag cgcgtcgctt caagggcaag
      541 aacgacacca tcgacgccga ggccgcagcg cgggcagtcc tgtcgggaga agcgaccatc
      601 gtcgccaaga cgggcgacgg cgtcgtggag cagatccggg ttgtgcgcgt ggcccgttgc
      661 ggggccatgc gggcccgcac gcaggcagcg aacgcgttga agtcgctcgt ggtcaccgcg
      721 ccagaccagc tgcgcgatca gctccgcagt ctcacaacga tccgcctggt ccgggccgca
      781 gcccgactcc gccccggcgc cgaccgcgac gtgacggccg ccacgaagct ggccatgcgc
      841 agcctcgccc gccgctacga atccctcgag gaagaaatca agatcctcga cgaagaactc
      901 cagcagctca ccacccaagc agcacccggc ttggtcgaga tgttcggcgt cggcaccgac
      961 accgccggcg cgctgctcgt cgccgccggc gacaacccgc aacgactccg atccgaagcc
     1021 gcgttcgcaa aactctgcgg cgtcgctccc atcgaagcat cctcaggcaa gaccgctcgg
     1081 catcgcctca accggggcgg caaccgcacc gccaacgccg cgctctaccg gatcgtgatc
     1141 gtgcggctcg gcctccacca gccgaccaag gactacgccg cgaagcgaac cgccgaagga
     1201 aaatcgaaga aggaaatcat ccggtgcctc aagcgacacc tcgtccgaga agtcttcgcc
     1261 gcgatcacaa agcaaccagc gatcgaagcc ggcctcgccg cttgacatct ataggagcat
     1321 cagcgagggg cacggtgccg gtacagggtt gcgtcgctgt ccaagcgaat ttcgagtcca
     1381 cgacgtggac tgatccattg cattgccccg gtacctgcga aaacgctgga gcccgaagcc
     1441 agagttgaac tggcgaccta cgcattacga gcggaagaaa cggggtcaac ggcgaacgtt
     1501 ggcgaacaaa aaccgcaggt cacagagggt gcgcgtacac cggcggacga ccccaaatga
     1561 ccgcgcatgt gccataaacg tgccatggaa ctcgcgatga ggcgtcaacg ccggcaggta
     1621 gcctgagaca cgtgagtgag catcagcgcg acaccactcc cccgcctgct ggggcgttga
     1681 cgatcgatga gttggcgcgt cttcagaacg ccgaggagac tcgggacgcg aaggaactgg
     1741 cagcggacat ctgggagtcc gacgcggagc ttgatgcctt cctcgccgac ctccggtcct
     1801 cccgcgacgc gtctctcgcc tgagccgtgg ccgatgtcgt catcgacacc gacgtggcgt
     1861 cggtactcca aaaggaccgc gcgccggctt gggtgggtcg gcacatcgcc ggcaagcggg
     1921 tgtggctgag tttcgtcgcc gtcggcgagc tgtggaaatg ggccgaggtg cggagttggg
     1981 gtgcgaagac gcgatcgagc ctcgacgact ggatcgccgc gagaccgatc gtgccttacg
     2041 acaccgaggt ggcgcgcagc tgggcgaggc tgtcagccgg ggcccaacgg cgcggccggc
     2101 cgactccgca gaacgacacg tgggttgccg cctgctgtgt ccgccacaac ctgcctctgc
     2161 tcacgctcaa caagaaagac ttcgagggct tcgctcgtca cgatggcctc gtgcttctcg
     2221 gggaggatga ctgacggttc caagaagcct gagctgccaa tcgggatgca gtgacaggtc
     2281 caaacaccgc ggcgagaggt cggcgtcgaa cagcagcctc acgcaggacg cgagatggga
     2341 agttcaccca cgtcgaccat cgccattgcg aactccagtg ccgcggtgat gtcctcgg
//
LOCUS       DATLCO010000007         3702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8949126, whole genome shotgun sequence.
ACCESSION   DATLCO010000007 DATLCO010000000
VERSION     DATLCO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8949126"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /gene="uvrB"
                     /locus_tag="VMZ33_00220"
     CDS             complement(<1..1841)
                     /gene="uvrB"
                     /locus_tag="VMZ33_00220"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HUR15681.1"
                     /translation="MPDFRLEAPFQPTGDQPQAIEKLVDGLGRGLKQQVLLGVTGSGK
                     SYTIASTIARHQRPSLILAHNKTLAAQLYSEMREFFPDNAVEYFVSYFDYYQPEAYLP
                     RSDTYIEKDSSRNDEIDKLRHAATRALFERRDTIIVASVSCIYGLGAPVDYGATVTRL
                     RVGGRYRRDGVLRQLVDLQYQRNDTALSRARFRVRGDTLELQPAYDDFIVRVQFFGDE
                     VERITELDPLTGEMLAERKELNVYPASHYVTPADKLKAAIVDIENELDVRVEELRQRG
                     MELEAERLRQRTTFDLEMMRELGFCSGIENYSRHLARREAGSRPWTLLDYFPPDWLLI
                     VDESHMTIPQVVGMYKNDRTRKEILVDFGFRLPSALDNRPLTFEEYEAHLNQVVYMSA
                     TPGAYELQRAEQIVEQLIRPTGIVDPEISVVPTEGQIDDLMDRIRDRVERGERVLVTT
                     LTKKMSEDLADYLHEMGVKVAYLHSEVDTLERVQILRDLRLGIYDVLVGINLLREGID
                     LPEVTLVAILDADKEGFLRSAWALIQMSGRAARNIGGEVVMYADRVTESMQVAIDETK
                     RRRAAQGKYNEEHGIVPQTIIKGIRDINDRLRAVAESSGAYNVEGQRR"
     gene            2150..3127
                     /locus_tag="VMZ33_00225"
     CDS             2150..3127
                     /locus_tag="VMZ33_00225"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HUR15682.1"
                     /translation="MEMEFKDPGRRRRFVLLMVGAVLAAAAGFAAFTMAGNKPSAAVI
                     TEAVLVAARDIPARQQVGVDDVTVRQVPIDEVLSQSYTETAMVVGRLTAVPVYTDQQM
                     TPNLFATSGGDTEYSILQADEQVTLTSPFWRAVSVRIPADRAVGGTLTTSQRVDLITS
                     VDFVIYGIDDEGNYQKIETATGEGLTTGKSTKLSFQDLEILKADPEEEMYVLKVDLHQ
                     AEQIAHIIQEAPDSMALALRPATDTRVANSLEYGETTDSLIMRYLFRVPRLADLSELL
                     GIPITPTAPTPTASPDPSATPDPNATPDPNASPAPSGSPEASPSASPAP"
     gene            complement(3356..3592)
                     /locus_tag="VMZ33_00230"
     CDS             complement(3356..3592)
                     /locus_tag="VMZ33_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15683.1"
                     /translation="MGHDRCTSRGAINSVGRNDAHVLSRLRHHETDAGMPESQPAKFT
                     VVGRMAALETRLHYNVVYRFSNVGDATTIEAPRL"
BASE COUNT          645 a         1221 c         1177 g          659 t
ORIGIN      
        1 cgccgctgac cctcgacgtt gtacgcaccc gacgactcgg cgaccgcgcg gagacggtcg
       61 ttgatgtccc gtatgccctt gatgatggtc tgcgggacga tcccgtgttc ctcgttgtac
      121 ttgccctggg ccgcccggcg ccgcttggtt tcgtcgatgg ccacctgcat cgactcggtg
      181 acacgatcgg cgtacatgac aacctcgccg ccgatgttgc gtgccgcgcg gccactcatc
      241 tggatgagcg cccatgccga gcgcaagaag ccctccttgt cggcgtcgag aatggccacc
      301 agcgtcacct cgggcaggtc gatgccctcg cgcaagaggt tgatgccgac cagcacgtca
      361 tagatcccga gccgcaggtc acgcaggatc tggacccgct cgagagtgtc aacctcggag
      421 tggagatagg cgaccttgac gcccatctcg tgcaggtagt cggccaggtc ctccgacatc
      481 ttcttggtca gggtcgtgac caggacgcgc tcgccgcgct ccacacgatc acggatgcgg
      541 tccatgagat cgtctatctg cccctctgtg ggcacgacgc tgatctccgg atcgacgatc
      601 cccgttggcc ggatcagctg ctcgacgatc tgttcggccc gctggagctc gtatgcgccg
      661 ggcgtcgccg acatatagac cacctggttg aggtgggcct cgtattcctc gaacgtgagc
      721 ggacggttgt cgagcgccga cggcaatcgg aagccgaagt cgaccaggat ctccttccgc
      781 gtccggtcgt tcttgtacat gcccacgacc tgggggatcg tcatgtgcga ctcgtcgacg
      841 atgagcagcc agtcgggcgg gaagtagtcg agcagcgtcc acggccgcga gccggcctcg
      901 cggcgagcca ggtggcgcga atagttttcg atgcccgagc agaagccgag ctcgcgcatc
      961 atctcgaggt cgaacgtggt ccgctgcctg agtcgttcgg cctccagctc catgcctcgc
     1021 tggcgcagct cctcgacccg aacgtccagc tcattctcga tgtcaacgat cgcggccttg
     1081 agcttgtccg ccggcgtgac gtagtgagac gcggggtaga cgttgagctc cttgcgctcg
     1141 gcgagcatct caccggtgag cgggtcgagc tcggtgatcc gctccacctc gtcgccgaag
     1201 aactgaacgc gcacgatgaa gtcgtcgtag gccggctgga gctcgagggt gtcgccacga
     1261 acgcggaacc gggcacgcga cagggccgtg tcgttgcgct ggtactggag atcgacgagc
     1321 tgccgcagaa ctccatcgcg gcggtagcgg ccaccgactc gcagccgcgt gaccgtggcc
     1381 ccgtagtcca ccggcgcgcc caggccgtag atgcacgaaa cgctggccac gatgatcgtg
     1441 tcgcggcgct cgaacaatgc ccgagtcgcg gcgtggcgaa gcttgtcgat ctcgtcgttg
     1501 cgcgacgagt ccttctcgat gtacgtgtcc gagcgcggca ggtacgcctc gggctggtag
     1561 tagtcgaagt acgagacgaa gtactccacc gcgttgtcgg ggaagaactc acgcatctcc
     1621 gagtacagct gcgcggccag cgtcttgttg tgcgcgagga tcagcgacgg ccgctggtgc
     1681 cgggctatcg tcgaggcgat cgtgtagctc ttgccgctac cggtaactcc cagcagaacc
     1741 tgctgtttga ggccgcgtcc gagcccgtca acgagctttt cgatcgcctg tggctgatcg
     1801 ccggtgggct ggaagggtgc ttcgaggcgg aagtcgggca tctctcgtaa agcgtaatgc
     1861 gaccctcgcg cggggcgccg gagtgtcgtg gagagcgcgg tgcagcggtc gcaggccaac
     1921 cgctgggtgg atgcggtcga ctctacatca gaacaggtgt tctatgccgc ccggcgcagt
     1981 gcggtgccgt tggtagcccc ttggtacccc accgccccac cggtgggacc ccttcctgat
     2041 accccgttgc gttcgtacct tctggtgcgc tgagacaacc caacgaccgg ctgccgctaa
     2101 cgccgagtcg ccatcaaagt cgataaccac ccgagggagc ctgccggcaa tggaaatgga
     2161 gttcaaggat ccggggcgac gtcgccgctt cgtgctgctg atggtcggcg ccgtgcttgc
     2221 cgctgccgcg ggcttcgccg ccttcacgat ggccggcaac aaacccagcg cagccgtcat
     2281 caccgaggcc gtgctcgtcg ccgcacgtga catcccggcg cgacagcagg ttggcgtgga
     2341 cgacgtcacg gtccgccagg tgcccatcga tgaggtccta tcgcagagct acaccgagac
     2401 ggcgatggtc gtcggccggc tgactgccgt gcccgtctac accgatcaac agatgacgcc
     2461 gaatctcttt gccacatccg gaggcgacac ggaatacagc attcttcagg ctgacgagca
     2521 ggtaaccctg acctctccct tctggcgcgc tgtatccgtc aggatccctg ccgaccgcgc
     2581 agtcggcggc acgctcacca caagtcagcg ggtagatctc atcacaagtg tcgactttgt
     2641 tatctacgga atcgacgacg aaggcaacta tcagaagatt gagacagcaa ctggtgaagg
     2701 cctgacgacc ggcaagtcca cgaagctctc gttccaggac ctggagatcc tgaaggccga
     2761 ccctgaagaa gagatgtacg tcctcaaggt cgacctacat caggcggaac agatcgccca
     2821 catcatccag gaagcgcccg actcgatggc gttggcgctg cgacccgcta ccgatacccg
     2881 cgtggccaac tcgctcgaat acggcgagac gaccgactcg ctgatcatga ggtacctgtt
     2941 ccgagtgccg cggctggcgg acctgtcgga gctccttggc atcccgatca cgccaacggc
     3001 acccactccg acagcatcac ccgatcccag cgctactccg gaccccaacg ccacgcccga
     3061 tccgaacgcc tcaccggcac caagcggatc gccagaggca tccccctcgg catcgcccgc
     3121 gccgtaagcc cttgcgacct gggtcgcaga acccgtacag cgcatcgccg cggtcaacct
     3181 ggccgcggcg attttgttgc cctggggccg gcgtgtttct ggaagtcgcg tcgctggggg
     3241 cactacgcgg agatcctcgc caacggccgg tgatgcaccc cctcgtcgaa gacgtgggga
     3301 ccggttcggc acgcggccgc catcgtcggc acttggctga gccagcaaga ccgagctaga
     3361 ggcgcggtgc ctcgatcgtg gtcgcgtcac ccacgtttga gaagcgatac acgacgttgt
     3421 agtgcagccg cgtctcgaga gcagccatac ggccgacgac cgtgaatttt gcgggttggc
     3481 tctccggcat gccagcgtcg gtctcgtgat gtcgaagacg cgacaggaca tgggcgtcgt
     3541 tgcgaccgac ggagttgatg gcaccgcgac tggtgcagcg atcgtgcccc acgagcctcg
     3601 ccaacgtcct cgacgtcgga cgtctccatt ccggcaaatg ggtgccttgt cgcggctggc
     3661 gagcgccacg tggccgtcga agtcctggcc cggaacgtcg cc
//
LOCUS       DATLCO010000008         4111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5226001, whole genome shotgun sequence.
ACCESSION   DATLCO010000008 DATLCO010000000
VERSION     DATLCO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5226001"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VMZ33_00235"
     CDS             <1..588
                     /locus_tag="VMZ33_00235"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HUR15684.1"
                     /translation="SWTDRQGNARANVLFLNPYDFPVQVTSSAARLINGSDETIKTGS
                     LYFLDGISGGSGYLLPAETIGANVCFTCEQALLTEDWASVGFSAFIRDATDDLAYSTE
                     VAATGVEVEFDGDSPIFWITGNAENTSASTLDRISVRVIVFDKDGSLIGAAESSGWDV
                     PAGDSVELTGGYGLGENPNGPFDIEVTALGVDYSN"
     gene            complement(635..>4111)
                     /gene="mfd"
                     /locus_tag="VMZ33_00240"
     CDS             complement(635..>4111)
                     /gene="mfd"
                     /locus_tag="VMZ33_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HUR15685.1"
                     /translation="ELRSLVDRYRTVREGRVGQNLRHVTYAQMPHGAKSFLAAALVVA
                     SGERLVWIARDAEIADRVAEEVASWLGGAGHVVTLEPRTSLAYERSELIRDESAARVA
                     ALATWRSGIPRVMVASVQALFQHTLAPDAIPHEPVTMRSGQRISQERVLRELLELGYE
                     ALPEVAGRGEFARRGGIVDVFPAGEPLPIRIEWFGDEIESLRGFDPANQRGVRPVDEI
                     TLLPASEFLIPQATSDRLRQSLGKGADRLSDDLAADLARLEQGQLADAAEIWAGHLAP
                     ATGLDHIGNELLVIDELPDVAASADFLWSQADERRGELQAAGILPKSWPTAYPQPREW
                     KQRLNVASTLELTWESEADGAPPGGNPFGWHEPVLPPSPIGDLAETIKRWRREGLRVV
                     LASDQSARLSEILGEADVIAAPIANLTEAPPAGGMALIERSLNSGFAGGPEQLVLVTD
                     RELFGTVRVRRPRVLRRVVPKDMLERLQPGDPVVHIDHGIAHYNGMVRRNAGGEGAEE
                     RDFLELHFAEGGRIWVPVEQIERVSRYAGGESPHLSRLGGGEWQRTKTRVRKAVTDLA
                     EELLALYTARDRANGRPVSPDSPWQGEMEAAFPYEETVDQLRATAEVKADLERGRPMD
                     RLVVGDVGYGKTEVALRAAFKAIADGLQVAVLVPTTVLAAQHLETFRQRFAAYPFSVR
                     MLSRFVAADHQREIIAGLAAGTVDLIIGTHRLLSKDIAFKNLGLVVVDEEQRFGVAHK
                     ERLKQMRTEVHVLTLSATPIPRTLNLALAGVRDMSVIETPPEDRLPIQTRVAEASAGL
                     VRDAILRELDRGGQAFFVHNRVETIEAQAQQLRNLLPQARIVAAHGQMGEGQLEKIML
                     AFARGDFDVLVCTTIIESGLDIPNANTIVIDRADTLGLAQLYQLRGRVGRSSRRAYAY
                     LLYRKRGQLSDIARKRLQAIFNASELGAGFQIALSDLEIRGAGNILGGEQHGHLAAVG
                     FDLYTRLLAEAVEERKAFVEHREPAKQRAQTVIDLPIDAYLPDEYVAEEPMKLELYRR
                     LGRTATNSEIETIRAELVDRFGATPAPVDRLLEVARLRIAAEAAGIASLAREGHELIV
                     RFNDDWSRSATMRAMAPTSVSDRIPGVATGGVTYGSNQMRIRVSRDAAAAWQTIRAVV
                     DRLANRVEKTAA"
BASE COUNT          685 a         1376 c         1369 g          681 t
ORIGIN      
        1 tcatggaccg atcgccaggg gaacgcccgt gccaacgtct tgtttctgaa tccttacgac
       61 tttccagtcc aggtgacatc gtcggctgcc aggctgatca acggttccga cgagacgata
      121 aagaccggat cgctctactt ccttgacggg atcagcggtg gcagtggcta cttgctaccc
      181 gccgagacga ttggcgcgaa cgtctgcttc acctgcgagc aagccctcct aacagaggat
      241 tgggcatcgg tgggattcag cgccttcatc agggacgcaa cagatgacct ggcctactca
      301 acggaggtgg cggcgacagg cgtcgaagtg gagttcgacg gcgacagccc aatcttctgg
      361 atcactggaa atgccgagaa cacgagcgcc tcgacattgg accgaatctc tgtccgcgtg
      421 atcgtctttg acaaggacgg cagcctcatc ggcgcagccg aaagctcagg ctgggatgtg
      481 cctgccgggg acagcgtcga gctaacgggc ggctacggac tcggcgaaaa ccccaacggg
      541 cccttcgata ttgaagtaac ggcccttggc gtggattaca gcaactgagg gctcggtcgg
      601 gaagctggtc gttgcagtag aagaaaaagg gccctcaggc ggcggttttc tcgacccgat
      661 tggcgaggcg atcaacgacg gcgcggatcg tctgccaggc ggcggccgcg tcccgtgaga
      721 cgcggatgcg catctggttc gacccgtacg tgacgccgcc ggttgcgacc cccgggatgc
      781 ggtccgagac cgatgtcggc gccatcgccc gcatggtggc tgagcgtgac cagtcgtcgt
      841 tgaagcgcac gatgagctcg tggccctcgc gtgcgagcga ggcgatgccc gcggcttcag
      901 cggcgatacg cagccgcgcg acctcgagca gccgatcgac gggtgccgga gtggcaccaa
      961 agcggtcgac gagctcggct cggatcgtct cgatctcgga gttcgtggcc gtccgaccga
     1021 gcctccggta gagctccagc ttcatgggtt cctcggcaac gtactcgtcc ggcagatagg
     1081 cgtcgatcgg cagatcgatg acggtctgcg cgcgctgctt ggctggttcg cggtgctcga
     1141 cgaaggcctt gcgctcttcg accgcctcag cgagcagccg cgtgtacagg tcgaagccaa
     1201 ctgcggcgag gtgaccgtgc tgttccccgc caaggatgtt gccggcaccg cggatctcga
     1261 ggtccgacaa ggcgatctgg aagccggcgc ccagctccga ggcgttgaag atggcctgga
     1321 gacgctttcg agcgatgtcc gacagctggc cgcgctttct atagagcagg tacgcgtacg
     1381 cccgacggga cgaccggccg acacgcccgc ggagctggta cagctgcgcc aaaccaaggg
     1441 tgtccgcacg atcgatgacg atcgtgttgg cgttggggat gtcaagcccg gactcgatga
     1501 tcgtcgtgca gacgaggacg tcgaagtcac cacgcgcaaa ggcgagcatg atcttctcga
     1561 gctggccctc ccccatctgc ccgtgcgcgg cgacaatccg agcctgcggc agcaggttgc
     1621 gcagctgctg cgcctgggcc tcgatcgtct cgacgcggtt atggacgaag aacgcctgtc
     1681 cgccccggtc gagctcacgc aggatcgcgt cacgtaccag gcccgcggac gcctcggcga
     1741 cgcgcgtctg gatcggcagg cggtcctccg gcggcgtctc gatgacgctc atgtcgcgca
     1801 caccggcaag tgccaggttc agcgttcgcg ggatcggtgt ggccgacagc gtgaggacgt
     1861 ggacctcagt gcgcatctgc ttcagccgct ccttgtgggc gacgccgaag cgctgctcct
     1921 cgtcgaccac cacgagtccc aggttcttga aggcgatgtc cttcgacagg aggcgatgcg
     1981 tgccaatgat caggtcgact gtgccggcgg ccaggccggc gatgatctcg cgctggtgat
     2041 ccgccgcaac gaaacgcgac agcatccgga cgctgaacgg gtaggccgcg aacctctggc
     2101 ggaaagtctc gaggtgttgc gcggcgagca cggtcgtcgg cacaagaacg gcgacctgca
     2161 gcccgtcggc gatcgccttg aatgcggcac gcaaggccac ttctgtcttg ccatacccga
     2221 cgtcgcccac gaccagccgg tccatcggcc gtccacgctc caggtctgcc ttcacctccg
     2281 ctgtggcacg tagctggtcg actgtctcct cgtacgggaa ggccgcctcc atctcccctt
     2341 gccagggcga gtcggggctg accgggcggc cgttggcgcg gtcacgggcc gtatacagcg
     2401 cgaggagttc ctcggcgagg tccgtcaccg ccttgcggac gcgcgtcttg gtgcgttgcc
     2461 actccccgcc gcccagccgc gacagatgcg gactctcgcc gccggcatag cgcgagacgc
     2521 gctctatctg ctcgaccggc acccagatgc gtccgccctc agcgaagtgc agctcgagga
     2581 aatcacgctc ctcggcgccc tcaccaccgg cgttgcggcg aaccattccg ttgtagtgcg
     2641 ctatgccgtg atcgatgtgg acgaccggat ccccgggctg gagacgctcg agcatgtcct
     2701 tcggcacgac acgcctgagc acccgcggcc gtcggacgcg gacagtgccg aacagctcgc
     2761 ggtccgtcac cagcactagc tgctccggac caccggcgaa gcccgagttg aggctgcgct
     2821 cgatcagcgc catgccgccc gcaggcggcg cctcggtgag gttcgcgatc ggggcggcga
     2881 tgacgtcggc ctcgcccagg atctccgaga ggcgcgccga ctggtcgctt gccaggacga
     2941 cgcgcagccc ttcccggcgc cagcgcttga tcgtctcggc caggtcgccg atcggcgacg
     3001 gtggcagcac gggctcgtgc cagccgaagg gattgccgcc tggcggcgcg ccgtcggcct
     3061 cgctctccca tgtcagctcg agcgtcgatg cgacgttcag acgttgcttc cactcgcgtg
     3121 gctgtgggta ggccgtgggc cacgacttgg gcaggatgcc ggctgcctgg agctcaccgc
     3181 ggcgttcatc agcctggctc cacaggaagt cggccgaggc agcgacgtct ggaagctcat
     3241 caatgaccag gagctcgttg ccgatgtgat ccaggccagt ggccggagcg aggtggccgg
     3301 cccagatctc ggcggcgtcg gcaagctgac cctgttcgag gcgcgccagg tctgcggcga
     3361 gatcgtcgga gaggcggtcg gcgcccttgc ccaacgactg ccgaagccgg tccgacgttg
     3421 cttgcgggat caggaactcg cttgcgggca gcagagtgat ctcgtcgacc ggcctcacgc
     3481 cgcgctggtt agccggatcg aagccgcgca gcgactcgat ctcgtcgccg aaccactcga
     3541 tgcggatggg aagcggctca cccgccggga acacgtcgac gatgccgccg cgccgcgcaa
     3601 actcgccccg tcccgcaacc tcgggaagtg cctcgtagcc gagctccagc aattcacgaa
     3661 gcacgcgttc ctgcgagatg cgctggcccg atctcatcgt gaccggctca tgcgggatcg
     3721 cgtccggtgc aagcgtgtgc tggaacagcg cctgtacgct ggccaccatc accctgggga
     3781 tgcccgaccg ccacgttgcc agcgccgcaa cccgcgcagc gctctcatcg cgaatcagct
     3841 cactgcgttc atacgccagc gaagtccgcg gttcgagcgt cacgacgtgg cctgcgccac
     3901 ccagccatga cgcaacctcc tccgcgacgc gatcggcgat ctctgcgtcg cgcgcgatcc
     3961 agacgaggcg ctcgccgctc gccacgacca gtgctgcggc caggaacgac ttcgcgccgt
     4021 gcggcatctg cgcgtacgtg acgtggcgaa ggttctggcc gactcgacct tcgcggacgg
     4081 tgcgatagcg gtccacgagc gaccgcagct c
//
LOCUS       DATLCO010000009         3721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8653, whole genome shotgun sequence.
ACCESSION   DATLCO010000009 DATLCO010000000
VERSION     DATLCO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8653"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VMZ33_00245"
     CDS             <1..727
                     /locus_tag="VMZ33_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR15686.1"
                     /translation="AARHAVDVADRLGAALRDARRKAGLTQADAARKAGISQSLWSQL
                     ESARSTGWTLLTWDRAAHAVGASFEAFIRGASAADQPRDIAHLKAQELVIRTSRAGLW
                     ASLPEQNIDRDARSSRAADVLLHRHIPPRPPEYGLMEVFDWFPDVGDPMRAWSSRLDA
                     VERLAIARMGDDLPQVGGCWIVRATQRNRQLFNELAGIFTARFPGDPNAWLRALADPG
                     SRMPKDAALLWVSVSGDRLFSRP"
     gene            complement(737..2038)
                     /locus_tag="VMZ33_00250"
     CDS             complement(737..2038)
                     /locus_tag="VMZ33_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUR15687.1"
                     /translation="MPCRNLALKARTDRRYIRSTYRSNRFILAEIAAPHAHREGGDRS
                     RPPVNLAFVIDRSGSMAGEKMRLAKEAVRQSIARLQADDQFSVVTYDDVVDVVVGSTR
                     ATPESRERALAQLATIDARNQTNLAEGWLRGCAQVAEHLLTDGVNRCLLLTDGLANVG
                     MTNPDELARHAGELRARGVSTTTFGVGADFDEVLLQAMATAGGGNFYYIADGRQIADY
                     VTSEVGEALDVVARDVRLEIVAPESAAVESLSPFPFESRGARTIVSLGSMVAEQVVQV
                     VLRVNLPLGESSRETGIVLSVAGASAVAAGSAEPSESVAISWEYADSKTNDAQERDAE
                     VDRAVARVFASRARQEATSLNHAGDYGRAAAALTSVAKRIRSYAGRDTEMRNLIASLD
                     ADAQQLAAPMPPAALKEMHFRSYRSAKTRDEMGRAMRRPQS"
     gene            complement(2041..2616)
                     /locus_tag="VMZ33_00255"
     CDS             complement(2041..2616)
                     /locus_tag="VMZ33_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUR15688.1"
                     /translation="MPNEYSLQELAKLSDVTPRTIRFYIEQGLLAAPTALGPRARYTD
                     DHLDRLRIIKKLQAGHLPLAEIRARLRGVAAGEHLPEPAQVNESALDYINQLLHPLTS
                     PPPPPPAASMAAPSAPAVAYRQLSVPAPSELPEPAKASEPTKTTEPERAQWERIALDP
                     DIEIHIRRPLTRQQNKRVERLISIARQLLEE"
     gene            2853..3344
                     /locus_tag="VMZ33_00260"
     CDS             2853..3344
                     /locus_tag="VMZ33_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15689.1"
                     /translation="MLAATSFGSGVWRIGADIPSGTYRTRLPATSCYWERLRGFSGDF
                     DDIIANDFSSGYHIVTIKGTDRGFSSSRCGRWSSDLSRVSTSMTKIGQGDFIPGVDFI
                     PGTYRSSKGDGCYWERLSGWTGDFSKIIANDFHSGGGLEVVTIRGTDRGFSSSRCGTW
                     TKI"
BASE COUNT          573 a         1236 c         1241 g          671 t
ORIGIN      
        1 tgctgcccga catgctgtcg acgttgcgga ccgtctcggc gccgcgctgc gggacgcacg
       61 ccgcaaggcc gggttgactc aggctgatgc tgcacgcaaa gcggggatca gccagagcct
      121 gtggagccaa ctcgaatctg ctcgctccac aggctggacg ctgcttacct gggatcgcgc
      181 ggcccatgct gtcggcgcga gctttgaggc attcatccga ggcgcctccg ctgccgatca
      241 gccgcgcgac atcgctcatc tcaaggcaca ggagctcgta atcaggacct cgcgagccgg
      301 cctgtgggcg agcctgccgg agcagaacat cgaccgcgac gcgcggtcct ctcgcgctgc
      361 ggacgtgctt ctccatcgtc acatcccgcc gcgccctccc gagtacgggc ttatggaggt
      421 cttcgattgg ttccccgacg tcggcgaccc gatgcgcgcc tggtccagcc gccttgacgc
      481 ggtcgagcgc ctggccatcg cacgtatggg tgatgacctt ccacaggtcg gcggctgctg
      541 gatcgttcgc gccactcagc gaaatcggca actcttcaac gagctcgcgg gcatcttcac
      601 ggcgcgcttt cctggcgacc ccaacgcgtg gctccgcgcc ctcgccgacc ccggctcccg
      661 gatgccaaaa gacgctgccc tgttgtgggt cagcgtctcc ggcgatcggc tattcagccg
      721 gccttaaggc ccgcgatcag gactgcgggc ggcgcatggc gcgacccatc tcgtcgcgcg
      781 tcttggccga ccgatagctg cggaagtgca tctccttcaa ggcggctggg ggcatcggcg
      841 cagcgagctg ctgggcgtcg gcgtcgagcg acgcaatgag gttccgcatc tccgtgtcgc
      901 ggccggcgta cgaccggatc cgtttcgcga ccgacgtcaa cgccgccgca gcgcggccat
      961 agtcgcctgc gtggttgagt gatgtcgcct cctgtcgagc tcgtgacgcg aacacccgag
     1021 ccactgcccg gtcgacctcg gcgtcgcgtt cctgcgcgtc gttggtcttg gagtcggcgt
     1081 actcccagga gatggcgacc gactcagacg gctcggcact ccccgccgcc acggccgagg
     1141 ctcccgccac cgacagcacg atcccggtct cgcggctgct ctcgcccagg ggcaggttca
     1201 cccgcagcac gacctggacg acctgctccg cgaccatcga ccccagcgac acgatagtcc
     1261 gtgccccgcg cgactcgaat gggaacggcg acagcgactc gaccgcggcc gactccggcg
     1321 cgacgatctc cagccgcacg tcgcgggcga cgacgtccaa cgcctcgcca acctccgacg
     1381 tcacgtaatc ggcaatctgc cgcccgtccg cgatgtagta gaagttgccg ccgccggcgg
     1441 tagccatggc ctgcaacagc acctcgtcga agtcggcacc cacgccaaac gtcgtcgtcg
     1501 acacgccgcg cgcccgcagc tcaccggcgt ggcgtgccag ctcgtcgggg ttggtcatgc
     1561 cgacgttcgc cagcccgtcc gtcaacagca ggcaccggtt gactccgtcc gtgagcaggt
     1621 gttcggccac ctgggcacat ccacgcagcc agccttccgc aaggttggtc tgattgcggg
     1681 cgtcgatcgt cgccagttgc gccaatgccc tttcgcggga ttccggtgtg gctcgcgtgg
     1741 agccgaccac cacgtcgacg acatcgtcgt aggtcacgac gctgaactgg tcgtcggcct
     1801 ggagccgggc gatcgactgc cgaaccgcct ccttggcaag gcgcatcttt tccccggcca
     1861 tcgaccctga gcggtcgatg acgaacgcga ggttcaccgg cggccgactg cggtccccgc
     1921 cttcgcgatg ggcgtgcggg gccgcgatct cggccaggat gaagcggttg ctgcggtagg
     1981 ttgaccggat gtagcgtcgg tcggtgcggg ccttaagggc caggtttcgg cagggcatgg
     2041 ctattcctcc aggagctggc gcgcgatcga gatcaggcgc tcgacacgct tgttctgttg
     2101 acgggtgaga gggcgcctga tgtggatctc gatgtcggga tcgagcgcga tgcgctccca
     2161 ctgggctcgc tcgggctcgg ttgtcttggt gggttcagac gctttggcgg gttcgggtag
     2221 ctcactcggc gccggcacgg aaagctggcg gtaagccact gcgggtgccg agggagcggc
     2281 catggacgcg gcaggcggag gaggcggcgg cgacgtaagc ggatgcagta gctggttgat
     2341 gtagtccagc gccgactcgt tgacctgcgc cggctcgggg agatgctcgc ccgctgcgac
     2401 tccgcgtagt cgcgcccgaa tctcagccag ggggagatgc cccgcttgca gtttcttgat
     2461 gatgcgcagg cggtccaggt gatcatccgt gtagcgcgcc cgagggccga gcgcggtcgg
     2521 cgccgcgagc aggccctgct cgatgtaaaa gcgaatcgtc cgtggcgtca cgtccgacag
     2581 cttggccagt tcctggaggg aatactcgtt gggcatcgcg gtcaatatga cagtgacacg
     2641 tcaccctgtc aagtacctgc gcctttggtg ctagccattc acgggcgctt cttccgcgaa
     2701 atacggtgaa cgccgtatgg gcgggccctt cggggacagg tactctgctt gccgaacgag
     2761 tcgcatccgc acctttcctt gagggggacc tattgcatcg ctttgtcgct gtcgcagctg
     2821 cgctgacggc ctgtcttctc atatcgagca ccgtgctcgc ggccacgtcc ttcggctcgg
     2881 gggtgtggcg catcggcgcc gacatccctt ccggtacgta ccgcacgcgc ctcccggcga
     2941 ccagttgcta ttgggagcgg ctgcgcggct tctccggtga cttcgacgac atcatcgcga
     3001 acgacttttc gtccggttac cacatcgtca cgatcaaagg caccgatcgc ggcttctcgt
     3061 cgagccgctg cggccgctgg tcgagcgacc tgtcgcgcgt ctcgacgagc atgacgaaga
     3121 tcggccaggg tgacttcatt ccgggcgtcg acttcatccc gggtacctat cggagcagca
     3181 agggcgacgg ttgttactgg gagcgtctca gcggctggac cggcgacttc tccaagatca
     3241 tcgccaacga tttccattcg ggcggcggcc tcgaggtcgt aaccatccgt ggcacggaca
     3301 gggggttcag ctcaagccgc tgcgggacct ggacgaagat ctagcctctt tgggcggccg
     3361 ataaggccat tcggacaaca cggatatcgg cactattctt accggccgcc gtcatgggtg
     3421 gccggtagtc ctttttgggc tggttccgag cgcgatcttc agtcagaagt gcagcgccga
     3481 gctcatttga gtgacttccg ggcgttcgca gccgagccag cggacaccat aagaatggtg
     3541 ccgatatcgt ggctgggagg gagcgaacgg ggagcgtcag ccgcggggcg gaggggggta
     3601 cgggcctcgc gaaccctacc ggtcagtcga gcaggcggtc cgctgctagc gcgaggcgca
     3661 cgactgtttc gggcccgaga gtggcctcgc cggagcgaac catcctggcc actgatcggc
     3721 c
//
LOCUS       DATLCO010000010         3565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8949640, whole genome shotgun sequence.
ACCESSION   DATLCO010000010 DATLCO010000000
VERSION     DATLCO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8949640"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1263
                     /locus_tag="VMZ33_00265"
     CDS             346..1263
                     /locus_tag="VMZ33_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HUR15690.1"
                     /translation="MRIATWNVNSLKARMDRLMWWLDRAQPDVLLIQETKLSDGDAPI
                     LPLRMRGYELVHHGEGRWNGVAIASRLPVDPSSIITNFGDGSVRDSGPGVATDFAAED
                     FNPFDEARMVSAVAGDIRFVCLYAPNGRVVGSVFYKGKLAWYDRLSRWLSENIEPGSA
                     AVVGGDMNVAPTPADVWDERAVHGGTHVSEPEREAFADLLSHGFIDGYRAKHDEPGRF
                     SWWDYRQGHFHKNMGMRIDHLLLTSPVAERLVWAEIDREARKGVPVPSDHAPVLIDID
                     EPGIALDPGWEGALERIAARTRPGRKFIG"
     gene            complement(1331..2074)
                     /locus_tag="VMZ33_00270"
     CDS             complement(1331..2074)
                     /locus_tag="VMZ33_00270"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HUR15691.1"
                     /translation="MDLGDTLSASFNQVVNDMVTALPAILGAIIILLVGYVIARVVSG
                     IVRGLLQRAGADKALAERGQQVYGARATSFKLSSIAGTIVFWVIMVVFLIAAANFLGW
                     PQVSDLLNGFIGWLPNLIVAVIILIAAPIIGRMVRGAVETGSAQMGMSSGSTLGRLAE
                     IGIIAFAVLIAINQVGIASDLVNILFIGLVAALALAFGLAFGLGGRDVAGQVTQDWYD
                     RSKGVAEKAQRQSTPASPTPTPTPPVRQG"
     gene            complement(2155..2922)
                     /locus_tag="VMZ33_00275"
     CDS             complement(2155..2922)
                     /locus_tag="VMZ33_00275"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HUR15692.1"
                     /translation="MSDQYVNEQSTSPYTGRPISEWLDEDIELHDMDGDEIGHVVEIN
                     PDFIVAQTSSGFLGLGEPKLYYIPRSSVAREEGDDWYLSIGKDEIESQQWNDAPTESA
                     WSSDWRDGQLTADEGRGQTRIRRYEEELEADTVQRESGEVVVNKRVVEETKTIEVPVR
                     REEVQIERRPVSGEAYTEGTIDQQAFGGESISIPLMAEEVEVRKVVRPVEEIEISKVQ
                     VQDTEQVEGTIRREEFDVDNTASSNSNNELADDESRR"
     gene            complement(3134..>3565)
                     /locus_tag="VMZ33_00280"
     CDS             complement(3134..>3565)
                     /locus_tag="VMZ33_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15693.1"
                     /translation="SKLTAVGALGLIAFVVAYMLYTPDQPIAYVIQESLLLAVAVGSF
                     VLPLRVMHDRLVAEKKRLLGDAQERLKQVLARIHVAVDANDLQPAEQLHHTLTVVLAE
                     IEVLNKLHTWPWSTATIRGFGSALLLPIALIVFTQFIDHLI"
BASE COUNT          630 a         1150 c         1138 g          647 t
ORIGIN      
        1 cgcgtgaaga cgctgatcgt ccgaccgtcg acacgggcgt ggtcgagcgg aacgatgtgc
       61 tcacgctcga gcagagtcgc gccctggatg cggtgactca ccgccatgcg gcgtgcgcgc
      121 tgacgcggcg ttcaatccgt ccgtcggcca cgatccacat gatcgctaca ccaacgcctc
      181 gagatgccct ctgctcacgc gcggaggtta gcctaggagc gtgccccgaa ccgacgagat
      241 cggcctcttg ctggagcggt atccaacctg cgtcgcggag caggtagatg agctgggccg
      301 tgtcgtgcgc cgcgttccac gcaggtcgct ccagggagca tcccgatgag gatcgcaacc
      361 tggaacgtca actcgctcaa ggcgcggatg gatcgcctga tgtggtggct ggatcgcgcc
      421 cagccggacg tcctgctcat ccaggaaacc aagctttccg acggcgatgc gccgatcctc
      481 cctctacgca tgcgcggcta cgagctcgtc catcacggcg agggccgctg gaacggcgtg
      541 gccatcgcgt cgcggctacc ggttgatccg tcgtcgatca tcacgaactt cggcgacggc
      601 tccgttcgcg atagcggacc gggcgtggcc actgatttcg cggcggagga cttcaacccc
      661 ttcgacgagg cgcgcatggt cagtgctgtc gccggcgaca tccgcttcgt ctgcctctac
      721 gccccgaacg ggcgcgtcgt cggatcggtt ttctacaagg gcaagctggc ctggtacgac
      781 cgactcagcc gctggctctc agagaacatc gagcccggct cggccgctgt tgttggcggt
      841 gacatgaacg tggccccgac ccctgccgac gtttgggacg agcgcgccgt gcacggcggc
      901 acacatgtgt cggagcccga gcgcgaggcc ttcgcggacc tgctcagtca cggcttcatt
      961 gacggctacc gcgcaaagca cgacgagccg ggccgcttca gctggtggga ctaccgccag
     1021 ggtcacttcc acaagaacat ggggatgcgg atcgaccatt tgctgctcac ctcccccgtt
     1081 gctgagcggc tggtgtgggc cgagatcgac cgcgaagcac gcaagggcgt gccggtcccg
     1141 tccgaccacg cgcccgtgct catcgacata gacgagcccg gcattgcgct ggacccgggc
     1201 tgggaaggtg cgttggagcg gatcgcggcg cggactcgtc caggcaggaa gttcattggc
     1261 tgagtgaaag aaagggtggg ccgacattta gccgacccac ccgtggaagc cgaaaggtgc
     1321 cgctggccgg tcagccctgg cggaccggcg gcgtcggcgt cggcgtcggc gaagctggcg
     1381 tcgactggcg ttgagccttc tcggcgacgc ccttcgaccg gtcgtaccaa tcctgtgtga
     1441 cctggccggc gacgtctcgg ccgccgagcc cgaacgcgag accgaacgcc agcgcgagcg
     1501 ccgcgaccag cccgatgaac aggatgttga cgaggtctga ggcgatgccg acctggttga
     1561 tcgcgatcaa caccgcaaag gcaatgatcc cgatctcagc caaacggcca agcgtcgatc
     1621 ctgacgacat gcccatctgc gcggagcccg tctcgacagc gccacgaacc atgcggccga
     1681 taatcggcgc ggcaatcagg atgatcactg cgacgatcag gttcggcagc cagccgatga
     1741 aaccgttgag caggtcgctt acctggggcc aaccgaggaa gttcgcggcc gcgatcagga
     1801 acacgaccat gatcacccag aacacgatgg ttccggcaat ggagctcagc ttgaagctgg
     1861 tcgctcgcgc tccgtagacc tgctggccgc gctcggccag ggccttatcc gcgcccgcac
     1921 gctgaagcag tccgcggacg ataccgctga cgactcgggc gattacgtac ccgacgagca
     1981 ggatgatgat cgcgccaagg atcgccggca gagccgtgac catgtcgttc acgacctggt
     2041 tgaaactcgc tgaaagtgtg tcacctagat ccacttggga tctcctttct gtatatggca
     2101 agacgggccg cgagccatcc ggccggcagc ccgcctagct ttgagggtta gtaactagcg
     2161 acgcgactcg tcgtcggcaa gctcgttgtt cgagttgctt gacgccgtgt tgtcgacatc
     2221 gaactcctca cggcgaatcg tgccctcgac ctgctcggtg tcctggacct gaaccttgga
     2281 gatctcgatc tcctcgaccg gtcgaacgac cttgcgaacc tcgacctctt ccgccatcag
     2341 cggaatgctg atcgactcgc cgccaaaggc ctgctgatcg atcgtgcctt cggtgtaggc
     2401 ctcgcctgat accgggcgtc gctcgatctg aacctcttca cgtcggaccg ggacctcgat
     2461 cgtcttcgtc tcttcgacga cgcgcttgtt gacgacgact tcgccgctct cgcgctggac
     2521 ggtgtcggcc tcgagctcct cctcgtaacg acggatccgc gtctggccgc ggccctcgtc
     2581 ggcggtcagc tgaccgtcac gccagtcgct cgaccatgcc gactcggtcg gagcgtcgtt
     2641 ccactgctgg gactcgatct catccttccc aatcgacagg taccagtcgt cgccctcttc
     2701 ccgggccaca gagctgcgcg ggatgtagta gagcttgggc tcgcccaggc cgagaaagcc
     2761 gctggatgtc tgtgccacga tgaagtccgg gttgatctca actacatggc cgatctcatc
     2821 gccgtccata tcgtggagct cgatgtcctc atcgagccac tcgctgatgg ggcggccggt
     2881 gtatggcgac gtgctctgct cattcacgta ctgatctgac ataggtctac ctccctatcc
     2941 cggaacaagc tgaaaacagc cgctggggct gtgcgtccgg gagttggtcc gcgcgcatga
     3001 ccggttctgg tgcgaacgcg aaccttcgat ttacggcgtg attaccgtac gagaggatcg
     3061 tggtcgcaga ccttgccgcg ccgtgacgtg aggtcacagc ggcgttgcac cggctagttg
     3121 gaggagccgg ggactagatc aggtggtcga tgaactgcgt gaagacgatc aatgcgattg
     3181 gcagcaggag cgccgacccg aagccgcgga tcgtcgccgt gctccacggc catgtgtgca
     3241 gcttgttcag cacctcgatc tccgcgagaa caacggtcag cgtgtggtga agctgttctg
     3301 ctggctgcag gtcgttggca tcgaccgcca cgtgaatccg ggcgagcacc tgcttgaggc
     3361 gttcctgagc gtcgcccagc aaacgcttct tctccgccac caggcggtcg tgcatcacac
     3421 gcagcggcag cacgaaggaa ccgactgcga ctgcgagcag gaggctctcc tggatcacat
     3481 acgcgattgg ctggtcgggc gtgtagagca tgtatgcgac gacgaacgcg atcaggccga
     3541 gggcgccgac cgcggtgagc ttcga
//
LOCUS       DATLCO010000011         4172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2244625, whole genome shotgun sequence.
ACCESSION   DATLCO010000011 DATLCO010000000
VERSION     DATLCO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244625"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="accD"
                     /locus_tag="VMZ33_00285"
     CDS             <1..967
                     /gene="accD"
                     /locus_tag="VMZ33_00285"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HUR15694.1"
                     /translation="PGHRLMPIKLPFGGRRPSHPTDLWTQCPDCQEMLYNKQLDKNQR
                     ICPRCGHHFRLRVDARLAQLLDRDSFREQDAGLQSVDILGFVDQKPYPERLEAAQAST
                     GVRDAAVWGTGTISGTRVVVCAMDFAFMGGSMGTVVGEKVTRAAEHALDERLPLIIVS
                     ASGGARMQEGTLSLMQLAKTVAALGRLADAGVPFISIMTDPTTGGVFASFSALGDVNI
                     AEPKALIGFAGARVAAGTIAEELPEGFQRAEFLFDHGFVDMVVPRPGLRETLIGLLRY
                     LKAPAAPNNGSGNGNGTSNGTSPLGRSLDFLETLAGREPKEPIHG"
     gene            960..1964
                     /locus_tag="VMZ33_00290"
     CDS             960..1964
                     /locus_tag="VMZ33_00290"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HUR15695.1"
                     /translation="MVDAAATVVNPEELQAAVWARVQLARNVHRPHTLELLRAMATDV
                     VELHGDRLFRDDPAIVGGFCKIDGRRAVFVGHQKGDEVNENVRRNFGMPHPEGYRKAI
                     RLFRLAERLQLPVVTFVDTPGAFPGPASEERGVAEAIAKAIQTMCGLRTPIVTVITGE
                     GGSGGALALAVGDVVIAFENAIYSVISPEGAASILWRSAEYAQQAAVAMRLTAAEQLA
                     LGVIDEVVPEPGGGAHENVNEAARNLRARIAYHLDALAGRDRRTLLEQRYARYRRMGE
                     FAVVTPSGDTRHQRGGLVERIREVLESGRAALRGRDGTGPLPTLSMDDDSAPPLREDV
                     "
     gene            1967..2452
                     /locus_tag="VMZ33_00295"
     CDS             1967..2452
                     /locus_tag="VMZ33_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HUR15696.1"
                     /translation="MPDQEARQVIARLTDEMVPRLIERLSKSDLAELEVREDGWRIRV
                     RRAVGAVDGAADVQPRAAAKSHARTTAVPVMSDQQAQKREQRAGTVQSPAVGYFTPRN
                     GVEGGATVRRGDPLGHVDVLGVRQEVVAPIDGVLKGFDVESGQAVEFGEPLARVEAGV
                     Q"
     gene            2442..3809
                     /gene="accC"
                     /locus_tag="VMZ33_00300"
     CDS             2442..3809
                     /gene="accC"
                     /locus_tag="VMZ33_00300"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HUR15697.1"
                     /translation="MFSKILIANRGEIALRILRACHRLGIASVVAYSEADRDSRAVQL
                     ADESICIGPAESRRSYLSAPALLSAALVTGAEAIHPGYGFLSEDETFAEMTRAHGLTF
                     IGPSPEVLERFASKAGTRTLLAKHGLPTIPGSGMLRDDAHALAEAEQIGYPVLLKPSA
                     GGGGKGMRMVRSPRELQQAITMCRSEAQAAFGDDSLYLEKWLEENRHIEVQVVVDRYG
                     NGVHVGDRDCSVQRRHQKILEEAPTPALDAAHRQALAESAIRAVIAAGYENVGTLEFL
                     LDRDGNYYFIEINCRIQVEHPVTEMLSGLDLVVEQIRIAAGEPLGYTQADVSLTGHAI
                     ELRINAEDSENDFRPQAGMVETYLPPGGPGVRMDSHLYSGYEVPPYYDSLLGKLVCWG
                     PDRDTAIARARAALDELVVDGLVTNVPLHRALLSHETFLNGEFTTNLLDRVGSGAFLA
                     AARAA"
     gene            3829..>4172
                     /locus_tag="VMZ33_00305"
     CDS             3829..>4172
                     /locus_tag="VMZ33_00305"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15698.1"
                     /translation="MTDTEVPDRNSAAADAVADVLKREGVLLNARDIQKLIPHRWPIM
                     MVDRIVEYDPSRQYIRGEKSVTATEWFFQGHFPGLPVMPGVLQVEALAQTMAIYVAQQ
                     EGFGDRIGLFAG"
BASE COUNT          699 a         1407 c         1406 g          660 t
ORIGIN      
        1 ccctgggcat cgactgatgc caatcaagct gcctttcggc gggcgccgac cgagccatcc
       61 gaccgatctc tggacgcagt gccccgactg ccaggagatg ctctacaaca agcagctcga
      121 caagaaccag cgcatctgcc cacgctgcgg ccaccacttc aggctgcgcg ttgacgcgcg
      181 cctcgcccag ctgctcgatc gcgactcgtt ccgagaacag gacgccgggc tccagtcggt
      241 cgacatcctc ggcttcgttg accagaagcc gtacccggag cggctcgagg cggcgcaggc
      301 ctcgacaggt gtcagggacg ctgccgtgtg gggcaccggc accatctccg gcacgcgcgt
      361 ggtcgtgtgc gcgatggact tcgccttcat gggcggctcc atgggcacgg tcgttggcga
      421 gaaggtcacg cgcgccgctg agcacgcgct cgacgagcga ctgccactca tcatcgtgtc
      481 ggcgtcaggc ggcgcgcgca tgcaggaggg aacactctcg ctgatgcagc tcgccaaaac
      541 ggtcgcggcg ctgggccgcc tggccgacgc cggcgtgccc ttcatcagca tcatgaccga
      601 cccgactacg ggcggcgtct tcgcgtcgtt ctcggcgctg ggcgacgtca acatcgccga
      661 gcccaaggcg ttgattggct tcgccggcgc gcgagtggca gcagggacga tcgccgagga
      721 gctgcccgag ggcttccagc gcgccgagtt cctcttcgac cacgggttcg tcgacatggt
      781 cgtcccacgg ccggggctgc gcgagacgct tatcggactg ctgcgctatc tcaaggcacc
      841 cgccgcaccc aacaacggca gcggcaatgg caacgggacc agtaacggca cgtcgcccct
      901 ggggcggtca ctcgatttcc tcgaaacgct cgctggtcgc gagccgaagg agcccatcca
      961 tggttgacgc ggcggccacg gtcgtgaatc cggaggagtt gcaggctgcc gtatgggcgc
     1021 gcgtccagct ggcacgcaat gtccaccggc cgcacaccct tgagctgttg cgcgcgatgg
     1081 cgacggacgt cgtcgagttg cacggggatc gccttttccg tgacgacccg gccatcgtcg
     1141 gcggattctg caagatcgac gggcgtcgcg ccgtcttcgt cggccatcag aagggcgacg
     1201 aggtcaacga gaacgtgcgg cgcaacttcg ggatgccgca tccagagggc taccgcaagg
     1261 cgatccgcct cttccggctg gccgaaaggc tgcagctccc cgtggtgacc ttcgttgaca
     1321 cgccgggcgc atttcccggt ccagcgagcg aggagcgggg cgttgctgag gcaatcgcga
     1381 aggcgatcca gaccatgtgt ggtctgcgaa ctccgatcgt gaccgtcatc accggcgagg
     1441 gcggctccgg cggagcgctg gcgctggccg tgggcgatgt tgtcatcgct ttcgagaacg
     1501 caatctattc cgtgatcagc cccgaggggg ccgcgagcat cctgtggcga tcggcggagt
     1561 acgcacaaca ggccgccgtc gccatgcgcc tgaccgccgc ggagcagctg gccctgggcg
     1621 tgatcgacga agtcgtgcct gagccaggcg gcggcgccca cgagaacgtc aacgaagcgg
     1681 cccggaacct tcgcgctcgg atcgcctacc acctcgatgc gctggccgga cgggaccgca
     1741 ggacgttgct cgagcagcgc tacgcgcgat atcggcgcat gggtgagttc gccgtggtca
     1801 cgccatcggg tgacacacgc caccagcgcg gtggcctggt cgagcgcatc cgcgaggtgc
     1861 tggagagcgg gcgtgcagcc ctgcgcggcc gagatggcac tggtcctctg ccgacgctca
     1921 gcatggacga cgactcagcc ccgcctcttc gcgaggacgt ctgacgatgc ccgaccagga
     1981 ggcacgccag gtgatcgccc gactcacgga cgaaatggtt ccgcgcctta tcgagcggtt
     2041 gtccaagagc gacctggcag agctggaggt ccgcgaggat ggctggcgca tccgcgtccg
     2101 tcgagcggtg ggcgcggtcg atggcgcggc cgacgtgcag cccagagcag cagccaagag
     2161 ccacgcgcgc accactgccg tcccggtgat gtccgatcag caggcacaga aacgcgagca
     2221 gcgtgccgga acggtccagt ccccagcggt gggctacttc acgccccgca acggcgtcga
     2281 gggtggcgcg accgtccgcc gcggcgatcc gctcgggcac gtggacgttc tcggcgttcg
     2341 gcaagaggtc gtggcgccga tcgacggagt gttaaaaggc ttcgatgtgg aatcgggcca
     2401 ggcggtcgaa ttcggcgagc cactggcaag ggttgaggcc ggtgttcagt aaaatcctga
     2461 tcgcgaatcg cggtgagatc gcgctgcgga tcctgcgcgc ctgccaccgg cttgggatcg
     2521 cgtcagtggt tgcctacagc gaggccgatc gcgactcccg tgccgtccag ctggccgacg
     2581 agtcgatctg cataggtccg gccgagtccc gccgcagcta cctgtcggcg ccggcactcc
     2641 tgtccgcggc cctggtcacg ggggcagagg cgatccaccc cggctatggc tttctctcgg
     2701 aggacgagac gttcgccgag atgacgcgcg cccacggctt gacgttcatc ggcccgtcgc
     2761 cggaagtgct ggagcgcttt gcgtccaagg ccgggacacg caccctgttg gcgaagcacg
     2821 gcctgcccac gattcccggc tcggggatgt tgcgtgacga cgcccatgca ctggcagagg
     2881 cggagcagat cggctacccc gtcctgctca agccgtctgc gggcgggggc ggcaagggca
     2941 tgcgcatggt gcgctccccg cgcgagcttc agcaggccat aaccatgtgc cgctcggagg
     3001 cccaggctgc cttcggtgac gactccctgt acctcgagaa gtggctcgag gagaatcgtc
     3061 acattgaagt ccaggtcgtt gtcgaccgct atggcaatgg cgtccacgtc ggcgaccgcg
     3121 actgctccgt ccagcgtcga caccagaaga tcctggagga agcgcctacc ccggcgctcg
     3181 atgcggccca tcgccaggcg ctcgctgaat cggcaatacg cgcggtcatt gccgcgggct
     3241 acgagaacgt cggcaccctc gagttcctcc tcgaccggga cgggaactac tacttcatcg
     3301 agatcaactg ccgcatccag gtcgagcatc ctgtgacgga gatgctctcc ggactcgacc
     3361 tggtcgtcga gcagatcagg atcgccgccg gcgagccgct cggctacacg caggccgacg
     3421 tgtcgctgac tggtcacgcc atcgagctgc gcatcaacgc cgaggacagc gagaacgact
     3481 tccgaccgca ggccggcatg gttgagacct acctgccgcc gggcggcccc ggggttcgaa
     3541 tggacagcca tctgtacagc ggctacgagg tgccgcccta ctacgattcg ctgctcggga
     3601 agctcgtttg ctgggggccc gaccgcgaca cagccatcgc ccgcgcccgc gcggcgctcg
     3661 atgagctggt ggtcgacgga ttggtcacca acgtgccact ccatcgagcg ctcctgtcac
     3721 acgagacatt cctcaacgga gagttcacca ccaacctgct cgaccgggtc gggagcggcg
     3781 ccttccttgc cgccgcccgc gcagcctgag cacccacgga gttaacactt gaccgatacc
     3841 gaagtccctg acaggaacag cgccgcggcc gacgcggtgg ccgacgtcct gaaacgtgag
     3901 ggcgtcctgc tcaacgcgcg ggacatccag aagctcatcc cgcaccgctg gccgatcatg
     3961 atggtcgacc ggatcgttga gtacgacccc tcccggcaat acattcgcgg cgagaagtcc
     4021 gtgaccgcca ccgagtggtt cttccagggc cacttcccgg gactgccggt gatgccgggc
     4081 gtgctccagg tagaggcgct cgctcagacg atggcgatct acgtggccca gcaggagggc
     4141 ttcggtgacc gcatcgggct gttcgcgggc at
//
LOCUS       DATLCO010000012         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2244635, whole genome shotgun sequence.
ACCESSION   DATLCO010000012 DATLCO010000000
VERSION     DATLCO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244635"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="accD"
                     /locus_tag="VMZ33_00310"
     CDS             <1..967
                     /gene="accD"
                     /locus_tag="VMZ33_00310"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HUR15699.1"
                     /translation="PGHRLMPIKLPFGGRRPSHPTDLWTQCPDCQEMLYNKQLDKNQR
                     ICPRCGHHFRLRVDARLAQLLDRDSFREQDAGLQSVDILGFVDQKPYPERLEAAQAST
                     GVRDAAVWGIGTISGTRVVVCAMDFAFMGGSMGTVVGEKVTRAAEHALDERLPLIIVS
                     ASGGARMQEGTLSLMQLAKTVAALGRLADAGVPFISIMTDPTTGGVFASFSALGDVNI
                     AEPRALIGFAGARVAAGTIAEELPEGFQRAEFLFDHGFVDMVVPRPRLRETLIGLLRY
                     LKAPAAPNNGSGNGNGTGNGTSPLGRSLDFLETLAGREPKEPIHG"
     gene            960..1964
                     /locus_tag="VMZ33_00315"
     CDS             960..1964
                     /locus_tag="VMZ33_00315"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HUR15700.1"
                     /translation="MVDAAATVVNPEELQAAVWARVQLARNVHRPHTLELLRAMATDV
                     VELHGDRLFRDDPAIVGGFCKIDGRRAIFVGHQKGDEVNENVRRNFGMPHPEGYRKAI
                     RLFRLAERLQLPVVTFVDTPGAFPGPASEERGVAEAIAKAIQTMSGLRTPIVTVITGE
                     GGSGGALALAVGDVVIAFENAIYSVISPEGAASILWRSAEYAQQAAVAMRLTAAEQLA
                     LGVIDEVVPEPGGGAHENANEAARNLRGRIAYHLDALAGRDRRTLLELRYARYRRMGE
                     FAVVTPSGDTRHQRGGLVERIREVLESGRAALRGRDGTGPLPTLSMDDDSAAPLREDV
                     "
BASE COUNT          341 a          707 c          723 g          332 t
ORIGIN      
        1 ccctgggcat cgactgatgc caatcaagct gcctttcggc gggcgccgac cgagccatcc
       61 gaccgatctc tggacgcagt gccccgactg ccaggagatg ctctacaaca agcagctcga
      121 caagaaccag cgcatctgcc cgcgctgcgg ccaccacttc aggctgcgcg ttgacgcgcg
      181 gctggcccag ctgctcgatc gcgactcgtt ccgagaacag gacgccggcc tccagtcggt
      241 agacatcctc ggcttcgtcg accagaagcc gtatccggag cggctcgagg cagcgcaggc
      301 ctcgaccggt gtcagggacg ccgccgtatg gggcatcggt accatctccg gcacgcgtgt
      361 ggtcgtgtgc gcgatggact tcgccttcat gggtggttcc atgggcacgg tcgttggcga
      421 gaaggtcacg cgcgccgctg agcacgcgct cgacgagcgg ctgccactca tcatcgtgtc
      481 ggcgtctggc ggcgcgcgca tgcaggaggg gacactctcg ctgatgcagc tcgcgaaaac
      541 ggtggccgcg ctgggccgcc tggccgacgc cggcgtgccc ttcatcagca tcatgaccga
      601 cccaacaacg ggcggcgtct tcgcgtcgtt ctcagcgctg ggcgacgtca acatcgccga
      661 gcccagggcg ttgattggct tcgccggcgc gcgagtggcc gcagggacga tcgccgagga
      721 gctgcccgag ggtttccaac gcgccgagtt cctcttcgac cacgggttcg tcgacatggt
      781 cgtcccacgg ccgaggctgc gcgagacgct tatcggactg ctgcgctatc tcaaggcacc
      841 cgccgcaccc aacaacggca gcggcaatgg caacgggacc ggtaacggca cgtcgcccct
      901 ggggcggtca ctggatttcc tcgaaacgct cgctgggcgc gagccgaagg agcccatcca
      961 tggttgacgc ggcggccacg gtcgtgaacc cggaggagtt gcaggctgcc gtatgggcgc
     1021 gcgtccagct ggcacgcaat gttcatcggc cgcacaccct tgagctgttg cgcgcgatgg
     1081 cgacggacgt cgtcgagttg cacggcgacc gccttttccg tgacgacccg gccatcgtcg
     1141 gcgggttctg caagatcgac gggcgtcgcg ccatcttcgt cggccatcag aaaggcgacg
     1201 aggtcaacga gaacgtgcgg cgcaacttcg ggatgccgca tccagagggc taccgcaagg
     1261 cgatccgcct cttccgcctg gccgaaaggc tccagctccc cgtggtgacc ttcgtcgaca
     1321 cgccgggcgc atttcccggt ccagcgagcg aggagcgcgg cgtcgccgag gcgattgcga
     1381 aggcgatcca gaccatgtct gggctgcgaa ctccgatcgt gaccgtcatc accggcgagg
     1441 gcggttccgg cggggcgctg gcgctggccg tgggcgacgt tgtcatcgca tttgagaacg
     1501 caatctattc agtgatcagc cctgaggggg ccgcgagcat cctgtggcga tcggcggagt
     1561 acgcacaaca ggctgccgtc gccatgcgcc tgaccgccgc ggagcagctg gccctgggcg
     1621 tgatcgacga ggtcgtgcct gagccaggcg gcggcgccca cgagaacgcc aacgaagcgg
     1681 cccgaaacct tcgcggtcgg atcgcctacc acctcgatgc gctggccgga cgggaccgca
     1741 ggacgttgct cgagctgcgc tacgcgcgct atcggcgcat gggtgagttc gccgtggtca
     1801 cgccatcggg tgacacacgc caccagcgcg gtggcctcgt cgagcgcatc cgcgaggtgc
     1861 tggagagcgg gcgcgcagcc ctgcgcggcc gagatggcac tggtcctctg ccgacgctca
     1921 gcatggacga tgactccgcc gcgccccttc gcgaggacgt ctgacgatgc ctgaccagga
     1981 ggcacgccag gtgatcgccc ggctcacgga cgagatggtt ccgcgcctta tcgagcggtt
     2041 gtccaagagc gacctggcag agctggaggt ccgcgaggat ggctggcgca tccgcgtccg
     2101 tcg
//
LOCUS       DATLCO010000013         4499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5226645, whole genome shotgun sequence.
ACCESSION   DATLCO010000013 DATLCO010000000
VERSION     DATLCO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5226645"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VMZ33_00320"
     CDS             complement(<1..626)
                     /locus_tag="VMZ33_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HUR15701.1"
                     /translation="MSLVWIIGVLPFLSTLLGGVAALRLHHRLHAVMALAAGILVATA
                     LVDLLPEAIELIGGEGAALTAGAAAVVGYLIYAAVEAFIHQGSYEHGHEPTQDPRAPH
                     EHATQTPMSGARSTLGWLAPSGLIVHSMLDGLAIGLGFEASDEVGIIVLLAVLVHDFA
                     DGLNVVTLALAGGRSRTAAMVLLAIDAVAIIVGIGLSQVIALAPDQLG"
     gene            complement(623..1519)
                     /locus_tag="VMZ33_00325"
     CDS             complement(623..1519)
                     /locus_tag="VMZ33_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HUR15702.1"
                     /translation="MLRSLAVVAASLAMAGCATADRTSVPTAAPQGGPITVLASTTVL
                     ADLVRRVGGNRVDVVTLIPAGTDTHTFGGTVDIESELDLVVWNGLGLDDETAVYAPDP
                     AHGLAIAESLDEVEMISDEQGNSNPHVWVDPANASVYLDRIRLTLIGIDPAGQATYDA
                     NTDSYLEQLERLEVDIRAQLDALPDENRSIYVTHDGLDYLAEFYGLEVLGTIYTGDDR
                     ELTADEQETLVAEIGSSGAQAVIGESHLPVEPAQSIATQAGVPWVQFVFTDSLGADPE
                     PDTYEEATRHNVDLIVAALTLP"
     gene            complement(1509..1946)
                     /locus_tag="VMZ33_00330"
     CDS             complement(1509..1946)
                     /locus_tag="VMZ33_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HUR15703.1"
                     /translation="MGSGTQGAIVDAVVRAGHRMTAPRRQVAELIAARQGHFTAANLL
                     DDASRRRLRLGRATVFRNLDLLAELDALERLDLPSGEHAYVTCAPEQHHHHVVCRSCG
                     RSVDVQDSGLQSVVNEIADRSGFKIDAHRLELFGTCPDCADAA"
     gene            2093..2737
                     /locus_tag="VMZ33_00335"
     CDS             2093..2737
                     /locus_tag="VMZ33_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HUR15704.1"
                     /translation="MPIYDRATALLVVDVQNDFADPAGNLYVKGGEDVVSLINREVAN
                     AHAAGAPVVYTQDWHPSTTPHFQKDGGIWPVHCVQDTWGAELHRDLSAQGPVVRKGSN
                     GEDGYSGFTMRDPETGATRPTELADILAEAAVTRLVVAGLATDYCVKSTVLDARAKGF
                     PTTVLLHAVRAVDLQPGDGDKAVEEMLAAGALLESRAILAGPIGTPAAPVVDHG"
     gene            2748..3866
                     /locus_tag="VMZ33_00340"
     CDS             2748..3866
                     /locus_tag="VMZ33_00340"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HUR15705.1"
                     /translation="MEQEADNRPFSPGLEGVLAGETALAKVDGANGRLTYRGYPIQDL
                     VRAGTYAQVAELLWTGEWPDEAHLPCAPLPEPILAALRALPSTAHPMDALRTATSVWG
                     GINRPGWPPTVEQARELTAMAPSALAAFGRLRQGLDPVDPDPSLQLAPGFLHQLTGET
                     PDPAAARALDAYFITGAEHGFNASTFTARVITSTQSDIASAVVGAIGALKGPLHGGAP
                     SEVVDQLQQIGSVEHAEQWLDEALDRGERLMGFGHRVYRAYDPRAAALREVAEGMASM
                     ATWLELAVAVEDVALRKLAERHPERPLKTNVEYYAAAVLQGVGLPSDLFPATFALARH
                     AGWTAHVLEQAQGNRLIRPDVRYVGPAERSLPGVGARS"
     gene            3907..>4499
                     /locus_tag="VMZ33_00345"
     CDS             3907..>4499
                     /locus_tag="VMZ33_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15706.1"
                     /translation="MRIRIGVALALLAIIAAPVANASAGPVAAADNLPDLELARPHDL
                     RVQVAPNNRKRLRFGSVVYNVGDGPIEVRASNRQGNVLTTVKQRIYRTDNTYHDVTKS
                     VRVSYEDDGHNHFHIRKFIVATLTRLDGTAPTRLLRKIGFCLVDTLQRSPTVPNAAGF
                     RFGNCGTRYSSSVVTGISVGWGDIYSPNTRYQQIDVTG"
BASE COUNT          765 a         1491 c         1507 g          736 t
ORIGIN      
        1 atgcccagct ggtcgggagc cagagcgatc acctggctga ggccgatacc cacgatgatc
       61 gccacggcgt cgatcgcgag cagcaccatg gcggctgtcc ggctgcgtcc gccagcgaga
      121 gccagagtca ccacgttcaa gccgtcggcg aaatcgtgta cgaggaccgc cagcaggacg
      181 atgatgccga cctcgtcgct ggcttcgaat ccgagcccga tcgcgagtcc gtcgagcatg
      241 ctgtggacga tgagcccgga cggcgcgagc cagccgagcg tcgagcgcgc gccgctcatc
      301 ggcgtctggg tggcgtgctc gtggggcgcc cgcggatcct gcgtgggttc gtggccgtgc
      361 tcgtagctgc cctggtggat gaatgcttcg acagcggcat agatgagata cccgacgaca
      421 gcggcagcgc cggcggtcag cgccgcgccc tcgccgccga tgagctcgat cgcttccggt
      481 aagaggtcga ctagggccgt ggccaccagg atgccggcgg cgagcgccat aaccgcgtgg
      541 aggcggtggt ggaggcgcag cgcggctacc ccgccgagga gcgtggacag gaagggcagc
      601 accccaatga tccagactag gctcatggca gcgtcagggc ggccactatc aggtccacgt
      661 tgtgcctcgt cgcctcctcg tacgtgtcag gttccgggtc cgcgccgagc gagtcggtga
      721 atacgaactg gacccacggc acgccggctt gcgtggcgat cgactgggcc ggctcaactg
      781 gtagatgcga ctcaccgatg accgcctggg caccggacga gccgatctcg gcgaccaggg
      841 tttcctgttc gtccgcggtg agctcccggt cgtcgcccgt gtagatcgtg cccagcactt
      901 cgaggccgta gaactcggcc aggtagtcga ggccgtcgtg cgtgacgtag atggagcggt
      961 tctcgtcggg gagagcgtcc agctgggcgc ggatgtcgac ctccaggcgc tcaagctgct
     1021 cgaggtagga gtcggtgttc gcgtcgtacg tcgcctgacc cgcgggatcg atgccgatca
     1081 acgtcagcct gattcggtcg aggtaaacgc tcgcgttggc tggatcgacc cacacgtgcg
     1141 ggttcgagtt gccctgctca tccgagatca tctcgacctc gtcgagactt tcggcgatgg
     1201 ccaggccgtg ggccgggtcc ggggcatata cggccgtttc gtcgtcaagc ccaaggccgt
     1261 tccacacgac gaggtcgagc tccgactcga tgtcgacggt tcctccgaac gtatgcgtgt
     1321 cagttcccgc cggtatgagc gtcacgacgt caacccggtt gccgccgacc cgtcggacga
     1381 gatctgccag gaccgtagtg ctggccagga cggttatcgg accgccctgg ggagccgctg
     1441 ttggcacgga tgttctgtcc gcggtcgcgc aaccggccat ggccaatgac gccgcgacga
     1501 cggcgaggct acgcagcatc ggcgcagtcc gggcacgtgc cgaagagctc cagccgatgg
     1561 gcgtcgatct tgaacccgct ccggtccgcg atctcgttca cgaccgactg gaggccgctg
     1621 tcctgaacgt cgaccgatcg accacagctg cggcatacga catggtggtg gtgctgctcg
     1681 ggcgcgcagg tcacgtacgc gtgttcgcct gaggggaggt cgaggcgctc cagggcatcg
     1741 agctccgcca ggaggtcgag attgcgaaag accgtcgcgc gacccaggcg caagcggcgg
     1801 cgcgacgcgt cgtcgaggag gttcgcggcg gtgaaatgtc cttggcgagc ggcgatgagc
     1861 tcggccacct gacgccgtgg cgcggtcatg cgatgaccag cacgaacaac ggcgtcgacg
     1921 attgcgccct gcgtaccgct tcccacgtac cagatgatac tcggtatcaa aagaaccgat
     1981 gggcgggcga atgttcctga gaagaccacc tcggcgaaca agcgcgcgat tcgagcgagg
     2041 cgggccccgt ccgccccagg aacattcggc tgccgcactt agaatccgag cgatgcccat
     2101 atacgaccgc gcgacggcgc tcctcgtcgt cgacgtccag aacgatttcg ccgatcccgc
     2161 gggcaacctc tacgtgaagg gtggcgagga cgtcgtttcg ctgatcaacc gcgaagtcgc
     2221 gaacgcccat gctgcaggcg cacccgtcgt gtacacgcag gactggcatc cgtcgaccac
     2281 gccgcacttc cagaaggacg gcgggatctg gcccgttcat tgcgtgcagg acacgtgggg
     2341 cgcggaactc caccgcgatc tatcggccca ggggcccgtc gtgcgtaagg gcagcaatgg
     2401 cgaagacggc tattccggtt ttacgatgcg cgacccggag accggcgcga ccaggcccac
     2461 cgagctcgcc gacatccttg ccgaggccgc cgtaacccgt ttggtcgtgg ccgggttggc
     2521 gaccgactac tgcgtgaaat cgaccgtgct cgatgctcga gcgaagggct tcccaacgac
     2581 cgtgttgctg cacgctgtcc gggccgtgga cctgcagccg ggcgacggtg acaaggccgt
     2641 ggaggagatg ctcgccgctg gggcgttgct cgagtcacgc gcgatcctgg ctggcccgat
     2701 cggcacgccg gccgcaccgg tcgtggacca cggctagact cggggagatg gaacaggaag
     2761 ccgataaccg accgttttca cccggcctcg agggtgtcct cgcaggcgaa acagccctgg
     2821 ccaaggttga cggcgccaac ggccgactca cgtatcgcgg ctacccgatc caggatctcg
     2881 ttcgcgccgg tacgtacgcc caagttgccg agctgctctg gaccggcgag tggcccgatg
     2941 aagcgcattt accgtgcgcg ccactgcccg agccgatcct cgctgcgctg cgcgcgctgc
     3001 cgtcgaccgc ccatcccatg gacgcgctcc gaaccgccac gtctgtgtgg ggtggtatca
     3061 accgaccggg ctggccgccg accgtcgagc aggcacgtga gctgacggcg atggcgccct
     3121 ctgcgctggc tgcgttcggc cgactccgcc agggattgga cccggtcgac cctgacccgt
     3181 ccctccagct ggcgccagga ttccttcatc agctcactgg cgagacgcct gaccccgccg
     3241 ccgcccgcgc cctcgacgcg tacttcataa ctggcgccga gcacggcttc aacgcttcaa
     3301 cgttcacggc gcgcgtgatc acatcaaccc agtcggacat cgccagtgcg gtcgtcggcg
     3361 cgattggcgc gctgaaggga ccgctacacg gcggggcacc gtctgaggtc gtcgaccagc
     3421 tgcagcagat cgggtccgtg gagcacgccg agcagtggct cgacgaggct ttggaccgcg
     3481 gcgaacgatt gatgggtttc ggccatcgcg tgtatcgcgc ctacgaccca cgcgcagccg
     3541 ccctacggga agtggctgag ggcatggcca gcatggcgac gtggctggag ctggccgtgg
     3601 ccgtcgaaga cgtcgcgctg cgcaagctcg ccgagcgcca ccctgagcgg cctctcaaga
     3661 ccaatgtcga gtactacgcc gcagccgtgc tgcagggcgt cgggctgcca tcggatctct
     3721 tcccggccac cttcgctctt gcgcgtcacg ctggctggac ggcacacgtc cttgagcagg
     3781 cacaggggaa caggctgatc cggcccgacg tacgctacgt tggcccggcg gagaggtcac
     3841 ttccgggggt gggcgctcga agctgacttg tcgcgtcggc gagacgagcc acgcgaggag
     3901 gggaacgtgc gaataaggat cggggtcgct ctggcactgc tggcgatcat cgctgcgccc
     3961 gttgccaacg catctgccgg ccctgtggcc gccgctgaca acctgcccga cctcgagctg
     4021 gcgcgcccgc acgacctgcg cgtgcaggtc gctcccaaca atcgcaagcg gcttcgcttc
     4081 ggctcagtcg tctacaacgt tggcgatggg cccatcgagg ttcgagccag caaccgccag
     4141 gggaacgtcc tgacgactgt caagcaacgg atctaccgga ctgacaacac ataccacgac
     4201 gtcaccaaga gcgtccgcgt ctcgtacgag gacgacggtc acaaccattt ccacatccgc
     4261 aagttcatcg tcgccacgct gacccgtctg gacggtacgg cgccaacgcg gcttttgcgg
     4321 aagatcggct tctgcctcgt tgacacgctt cagcggtcac cgaccgtacc taacgccgca
     4381 ggtttcaggt tcgggaactg tggcaccagg tacagctcga gcgtcgtcac cggcatctcg
     4441 gttggctggg gggacattta ctcgcccaac actcgctatc agcaaatcga cgtgaccgg
//
LOCUS       DATLCO010000014         3838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5968445, whole genome shotgun sequence.
ACCESSION   DATLCO010000014 DATLCO010000000
VERSION     DATLCO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5968445"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VMZ33_00350"
     CDS             <1..660
                     /locus_tag="VMZ33_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15707.1"
                     /translation="VSDLDSDEALVNFGTPAYSYGHEVYDDLAVTSNGYVVVGTGTSE
                     DLDYVPQDFPDPARPNNVIAPYWTDLDPASGGDIRIGTLTDGVDTWIVVDWQDVHVYG
                     TTNDRSFQVWIQIGGTAEEGIWLEHDADAVAAAGASDGLIVGAENRDGSSGADLGMDV
                     PPANTGYRVITGNPTAGGSKTITYEALGQARGAYYILASMTSDITQGTATDRDRIRVT
                     P"
     gene            complement(750..1325)
                     /locus_tag="VMZ33_00355"
     CDS             complement(750..1325)
                     /locus_tag="VMZ33_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HUR15708.1"
                     /translation="MLAPVMNRLMLVGTMVLALGCSPVVVQSPPVVATRHPVASASAT
                     VVASTFAPSGPTEEAEVVRVVDGDTIVVDLGGFEERVRYIGIDAPESVRPSFPVEEWG
                     VEASAANADLVEGRTVVLERDVSDTDRFGRLLRYVWLLNDGEWLLVNLELVRLGYANA
                     GSFPPDVKYNDEVRAAEREARDADRGLWGLP"
     gene            1587..3197
                     /locus_tag="VMZ33_00360"
     CDS             1587..3197
                     /locus_tag="VMZ33_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15709.1"
                     /translation="MMSLGRFPTLLSLLAVLACVVAIPESVHAQTPPGGPGGVAASWT
                     QPGQPGDLNGSVKAFATIGSDLYMAGQFTNVGGDPAADYVVRWDGTDWHALGSNGSGD
                     GALGAATYDLAVMDDDLYVAGSFLNAAGIPQADNLARWDGAAWSAVGGYHGNGVFNGF
                     VAALAVSGDRLYAGGSFQDVAGKPAADNIARWNGRTWSAIGSDGNGNGAVGSFIYDIA
                     VGRSGIYAAGGFQNAGGHPEADHVARWNGSEWLPLGSNGAGGPAINALVHALVIHGDD
                     LFVGGAFVNAGGNPRSDYVARWGAGSWHNLGSNGAGNGRLTDAVGALIFWAGQLYVGG
                     WFRDMRNLDADYLARWTGTGWEGLDYWDYTGGAVSPTMGLAHTTDHLFVGTNANFMPP
                     VFEYGPLPHYLPDAQIDGTGNNVHNSTGADQTRSARVRPGSAFTFNVLLQNDGIKPDT
                     LRLSAEDVASPGFTVTYRQGTTDITAAVLAGTYESPLIGPGDAFLVRAKVSVAGDAPA
                     ASGITRLVTATSVNDAASDTVRFRVSTP"
     gene            complement(3205..>3838)
                     /locus_tag="VMZ33_00365"
     CDS             complement(3205..>3838)
                     /locus_tag="VMZ33_00365"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HUR15710.1"
                     /translation="RAAEYAREARTAGAHSVMLSAPPLARPNDDAVRRHYLRVAEAVP
                     ELPVVIQDLPTLGVWMSADLIAGMAREAPNLRVVKLEEEPTAPKVGRLLAANDELRVL
                     GGLGGQMLIEELRRGAVGTMTGFGYPETLVAIMGDWRAGRIDAAVARFHEWMPLIRFE
                     NQQLLNLPIRKHIYMRRGAIASDKVRAPGPTLEEGTRADLDDLLLRLGVD"
BASE COUNT          678 a         1261 c         1263 g          636 t
ORIGIN      
        1 gtcagcgacc tcgacagtga cgaggccctc gtaaacttcg ggacaccggc atacagctac
       61 ggccatgagg tgtacgacga cctcgccgtc acgtcgaacg gctacgtggt agtcggcacc
      121 gggacgagcg aagacctgga ctacgtaccg caggacttcc cggatcccgc gcggccgaac
      181 aatgtcattg ccccctactg gacggacctt gatcccgcat cgggcgggga catccgtatc
      241 ggcaccctca cggatggggt agacacgtgg atagttgtcg actggcagga cgtccatgtg
      301 tacggcacga ccaacgatcg cagtttccag gtgtggatcc aaattggcgg tactgccgaa
      361 gagggcatct ggctggagca cgatgcggac gcagtcgcgg cagccggcgc gtctgacggc
      421 ctgatcgtag gggcggaaaa ccgggacggc tccagtggag ctgatcttgg catggacgtt
      481 ccgccagcaa ataccggcta tcgggtgata acgggcaacc cgacggcggg tggctctaag
      541 acgatcacct atgaagcgct cggccaagcg aggggtgcgt actacatcct cgcttcgatg
      601 acgagcgaca ttacgcaagg cacggccacg gatcgcgaca ggatcagggt cacgccatag
      661 ttcacattcg gtaacgaatg cctaccgggt cgcggtaggg gcgcctagag ggtcggcaga
      721 aatgccggcc ctcttttatt tggaagcggt cagggcaatc cccagaggcc gcggtctgcg
      781 tcgcgcgcct cgcgttcggc ggcgcgcacc tcgtcgttgt acttcacgtc cggcgggaag
      841 ctgccggcgt tggcgtaacc cagcctgacg agctccaggt tcacgagtag ccactccccg
      901 tcgttgagca accagacgta gcggagcagt cggccgaacc gatcggtgtc ggagacgtcg
      961 cgctcgagga cgacggtgcg accctcgacc agatctgcgt tggccgccga cgcctcgacg
     1021 ccccactcct ccacggggaa gctgggccgg accgactcgg gggcatcgat gccgatgtag
     1081 cgcacccgct cctcgaaccc cccgaggtcc acgacgatcg tgtcgccgtc cactacgcgg
     1141 actacctccg cctcctcagt cgggccggac ggcgcaaacg tgctggccac gacggtggcg
     1201 ctcgctgatg caacggggtg ccgcgtcgcg acgacgggtg gcgactggac aaccactggc
     1261 gagcagccca acgcgaggac catggtgcca acgagcatca aacggttcat gaccggcgct
     1321 agcatgggcc accgatgaga aacgttgtgc tggcagttgc ctcgccggtc atcgcgcggc
     1381 gcggggcggc tccactcggg cgcaggcgaa ccctactaat gccgcttcaa ggctcgaaca
     1441 ccctccgagc cgcgtggggt actgacgcgg ctcttcagta gaccagcggg tgccagcacg
     1501 gagggcgccg acggggtgtg ggcgtaaggt cgagcgacac ctcgcggtcg ccgtcctcat
     1561 tggccgcgga ggaaggagtc tttggcatga tgagcctcgg ccggtttccg acattacttt
     1621 cgctcttggc ggtccttgcg tgcgtggtcg cgatccccga gtcggtgcat gcgcagactc
     1681 cccccggcgg ccctggcggc gtggcggcgt cgtggactca acctgggcaa ccgggcgacc
     1741 taaacggttc ggtgaaggca ttcgccacca tcgggtctga cttgtacatg gccggccagt
     1801 tcacaaacgt cggcggcgat ccggcggccg attacgttgt cagatgggac gggacggact
     1861 ggcatgcgct cggatcgaac ggatcaggcg acggtgcact cggtgcggcg acttacgacc
     1921 tggcagtcat ggatgacgac ttatacgtcg cggggagctt cctcaacgcg gcagggattc
     1981 cacaagcgga caaccttgcg cgatgggatg gagcagcttg gtcggcggtc ggcgggtatc
     2041 acgggaacgg cgtgttcaat gggtttgtcg cggcactcgc cgtgtcgggc gatcggctct
     2101 acgcaggcgg gtcgttccag gacgtagccg gcaaacctgc agccgacaac atcgcccggt
     2161 ggaatgggcg gacatggtca gcgatcggat cggatggcaa cggtaacggt gcggtcggaa
     2221 gtttcatcta cgacatagcc gtcggtagga gtgggattta cgcggctggc ggcttccaga
     2281 acgcgggtgg ccatcccgaa gccgaccatg tcgcgcgttg gaacggcagc gaatggctgc
     2341 cactgggctc aaacggcgcc ggcggtccag caatcaatgc gctagttcac gcgctcgtga
     2401 tccacggcga cgatctcttc gttggcggtg ccttcgtcaa cgccggtggc aaccccaggt
     2461 ccgactacgt ggcccgctgg ggcgcgggtt cgtggcacaa cctgggcagt aacggcgccg
     2521 gcaacgggcg gctgaccgac gctgtcggcg ccctcatctt ctgggctggg cagctgtatg
     2581 tcggtggttg gttccgcgac atgcgcaatc tcgatgccga ctatctcgcg cgctggacgg
     2641 gcacgggctg ggaaggcctc gactactggg actacaccgg gggcgccgtt tcgccgacga
     2701 tgggcctcgc acataccact gaccacctgt tcgtcggcac gaacgccaat ttcatgccgc
     2761 ccgtgttcga atacggtccc ctgccgcact acctgccaga cgcccagatc gatggcacgg
     2821 gcaacaacgt ccacaactcg acaggcgcgg atcaaacgcg ctcggcgcgc gtccgcccag
     2881 gctcggcgtt cacgttcaat gtcttattgc agaacgacgg catcaagcca gacacgctga
     2941 ggttgagcgc cgaagacgtc gcatcgcccg gcttcacggt cacataccgc cagggcacga
     3001 ccgacatcac cgccgcagtc ctcgccggca catacgaatc gccgttgatc ggccccggcg
     3061 atgcatttct cgtgagagcg aaggtgagcg tggccggaga tgctccagcc gcttcaggaa
     3121 tcacccgtct ggtgacggca acctcggtca atgacgcagc gagcgacacc gtgaggttcc
     3181 gcgtctcgac cccgtagacg aacgctaatc gacgcccagc cggagcagca ggtcgtccag
     3241 gtcggctctt gtcccctcct cgagggtcgg gcccggcgct cgcaccttgt ccgacgcgat
     3301 ggcgccccgc cgcatgtaga tgtgcttccg gatcggcagg ttgagcagct gctggttctc
     3361 gaatcggatc agcggcatcc actcgtgaaa gcgcgccaca gccgcgtcga tccgccccgc
     3421 gcgccagtca cccatgatcg cgacgagcgt ctccgggtaa ccaaagccgg tcatcgtccc
     3481 gaccgcgccg cgccgcagct cctcgatcag catctggccc ccgagcccgc ccagcacacg
     3541 cagctcgtcg ttagccgcca gcagccgccc gactttgggc gcggtcggct cctcttcaag
     3601 cttcaccacg cgcaggttcg gcgcctcgcg tgccatgccg gcgatgagat ctgcgctcat
     3661 ccacaccccg agcgtcggca gatcctggat gaccaccggc agctccggca cagcttccgc
     3721 cacccgcagg taatggcgcc gcacggcgtc atcgttcggc cgcgctagcg gcggtgccga
     3781 cagcatcacg gagtgcgccc cggcagtccg tgcctcgcgt gcgtactcgg cggcccgg
//
LOCUS       DATLCO010000015         3298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2247764, whole genome shotgun sequence.
ACCESSION   DATLCO010000015 DATLCO010000000
VERSION     DATLCO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247764"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VMZ33_00370"
     CDS             <1..619
                     /locus_tag="VMZ33_00370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HUR15711.1"
                     /translation="PSAPARVRYVALGDSYTIGTSVDASDRFPDQLVARLAGQVDLEL
                     VANLGVNGYTTDDLIADELPELPDLAPDFVTLLIGVNDDVRGSSMDRYRSNLDLILGR
                     ILREVPAARIVVVSIPDYTLTPAGGNFGDPVQQSAAIAQANAIMLAAADVRGIAFVDI
                     SAAANQAGVDRTLVANDGLHPSGAQYGLWVDLIEPVVIDLLAQAE"
     gene            complement(591..1127)
                     /locus_tag="VMZ33_00375"
     CDS             complement(591..1127)
                     /locus_tag="VMZ33_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15712.1"
                     /translation="MAYSTREPFGTSGFQGGFIEGVKRMGRPLVILSLALFAIACSVR
                     PPVPPDFMVPIPAHGFGDFVVEVYDSAELLVDARARDVGPADVGAAGTLVSRPDRNEL
                     DVSWTGGACALRPLINVRGAPDNLVIELVPDPTDINLVVVPQECPAIGLPFGVTLTLS
                     EPVEQANVTLTQPEQEDR"
     gene            1167..2051
                     /locus_tag="VMZ33_00380"
     CDS             1167..2051
                     /locus_tag="VMZ33_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15713.1"
                     /translation="MNLTMPWTASRKTPVNGVTSKLDEIRNALSNEADRLAEVAAQFG
                     REASGTVAKIAEESVSSASKLSHDAVSQAAKIGADAGDKASSAVKDPVGTTTSLGQSL
                     VRGIADVGAALASSGKQTAKDLSKDANTVARDLRKVRITTDAKQSKPDALPGITLLAG
                     FGTGIALMYFFDPERGKRRRAMLREQLTKWTRMGRDTVQGKARDLQNRAAGARSAAAG
                     LTHDGQDSEDVQDMFAETGFGSDPVITDSRAGQPFGQENGSTDYGSTDYGTPSEVTTE
                     SWEEQQTEESRSSRIEIG"
     gene            2151..>3298
                     /gene="carA"
                     /locus_tag="VMZ33_00385"
     CDS             2151..>3298
                     /gene="carA"
                     /locus_tag="VMZ33_00385"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HUR15714.1"
                     /translation="MTYRDHRSYESGGLFSAISVPFDSVVDPRIGANGPALLALEDGT
                     VFHGVSFGAPVTGGDDLVANTGYSGYQEICTDPSYAGQVVLMTYPLIGNYGRIELDDQ
                     STRPWLRGLIVSHATAAVLEPARQLVDMLRSAGVPAIAGLDTRRLARRLRETGSQRVL
                     ITAPGDLNVDSAVAGAQRLARWEDEDFVAQVSAVEPYEIGPADGPVIALIDFGLKVNI
                     CRQLVARGARVRVLPHTTSAATALAPDVRGLVFSPGPGDPARLHAPVRLAQAAIDDGR
                     PLLGICLGHQIVGRAAGAETRRLRFGHHGANHPVKDVATGKVQVTAQNHEVEVVGETL
                     PLQSGFEVSQRNLNDGSVEGLRHEHKPIETVQYHPEGSPGPLDALDVF"
BASE COUNT          592 a         1094 c         1080 g          532 t
ORIGIN      
        1 cccctctgcc ccggcgcgag tccgttatgt cgcgctgggt gactcgtaca cgatcgggac
       61 cagcgttgat gcgtccgatc gctttcccga tcagctcgta gcgcggctgg ccgggcaggt
      121 ggacctggag ctggtggcca atctgggcgt gaacggctac acaaccgacg acctcatcgc
      181 cgatgagcta cccgagctgc cggatctcgc gccggatttc gtgaccctgc tgatcggcgt
      241 caacgacgac gtccgtggct cttcgatgga tcgataccgc tcgaacctcg acctcatcct
      301 cggacgaatc ctcagggagg tgccggccgc gcgcatcgtc gtggtctcga tccccgacta
      361 cacgctcacc ccggccggcg gcaatttcgg cgaccccgtg cagcagagcg cagccattgc
      421 tcaagccaac gcgatcatgc ttgccgcggc cgacgtccgt ggcatcgcgt tcgtcgatat
      481 ctccgccgcg gccaatcagg ccggcgtcga tcgcacactc gtggccaatg atggtctcca
      541 cccgagcggg gcgcaatacg ggctgtgggt cgacctgatc gagcccgtcg tcatcgatct
      601 tcttgctcag gctgagtgag tgtgacgttg gcctgctcga ccggctcgga tagcgtgagc
      661 gtcacaccga acggaaggcc aatcgccggg cactcctgag gaacgacgac gagattgatg
      721 tccgtcgggt ctggcacgag ctcgatgacg aggttgtcag gggcgcctcg aacgttgatc
      781 agcggccgaa gggcgcatgc gccgccggtc cacgagacat ccagctcgtt cctatcgggg
      841 cggcttacca gcgtgcccgc cgctccgacg tctgcaggac caacgtcgcg cgctcgggca
      901 tccaccagca gctccgcgga gtcgtagacc tccacgacga aatcaccgaa tccatgggcg
      961 ggtatcggga ccatgaagtc cggcgggacc ggcggtcgta ccgaacaggc aatcgcgaag
     1021 agggccaggc tgaggatgac caatgggcgc cccatgcgtt tcacgccctc aatgaaacca
     1081 ccctgaaacc cgctggtgcc gaacggctct cgtgtgctgt acgccacgca ggcgatcatg
     1141 gacgcatcac ccaaggaggg accaggatga atttgacaat gccctggacc gcgtcaagaa
     1201 aaacgccggt caacggggtc acctcaaagc tagacgagat tcgcaacgcg ctgagcaacg
     1261 aggccgatcg actggccgag gttgcagcgc agttcggtcg cgaggcctca ggcaccgtgg
     1321 ccaagatcgc tgaagaaagc gtttcttcgg catcgaagct cagccacgac gccgtgtcgc
     1381 aggctgccaa gatcggcgcc gatgcgggcg ataaggcaag ttctgccgtc aaggaccccg
     1441 tcgggacgac gaccagcctc ggccagagcc tcgtccgcgg tatcgccgac gtcggcgctg
     1501 cccttgcttc gtcgggcaag cagactgcca aggacctgtc gaaggacgcc aacacggtcg
     1561 ctagggacct gcgcaaggtc cgcatcacga ctgatgccaa gcagtccaag cccgacgcgc
     1621 tgccgggcat cacgctgctg gccggtttcg gtactggcat cgcgctcatg tacttcttcg
     1681 accccgagcg tggcaagcga cgccgcgcga tgctccgtga gcagctcaca aagtggacgc
     1741 gcatgggccg cgacaccgtc cagggcaagg cccgggatct tcagaaccgt gctgccggcg
     1801 ctcggagcgc cgcggccggg ctgacccacg acggccagga ctcggaagac gtccaggaca
     1861 tgtttgctga gaccggattc ggctcagacc ccgtgattac ggacagtcgt gccgggcagc
     1921 cattcggcca ggaaaacggc tcgaccgact atggctcgac cgactacggc acgccgtccg
     1981 aggtaacgac cgagtcatgg gaagagcagc agactgagga gtcgcgcagc tcgcgcattg
     2041 aaatcggctg acgcctccgt gctaacctga agaggtccct ttcaccggtc cagagaggcc
     2101 ggcgaggacg gctccgagag agccgggaag gagtcaggaa agccttgtcc gtgacataca
     2161 gagaccaccg ctcgtacgag agcggtggtc ttttttctgc gatatccgta ccgttcgact
     2221 ccgttgtcga tccacgcatc ggtgccaacg ggccggcact actcgccctg gaggacggga
     2281 cggtctttca cggggtctcg ttcggggccc ctgtcaccgg cggcgacgac ctcgtggcaa
     2341 acaccggcta cagcggctac caggagatct gtaccgatcc ctcgtacgct ggccaggtcg
     2401 tgctcatgac ctacccgctg atcggcaact acgggcgtat cgagctcgac gaccagtcca
     2461 ctcgaccgtg gctccggggg ctcatcgtca gccacgcaac ggcggcggtg ctcgagccgg
     2521 cgcgccagct cgttgacatg ctccgctctg cgggagtgcc cgcgatagcc ggcctggaca
     2581 cgcgccggct ggcgcgccgg ctgcgcgaaa cggggtcgca gcgcgtcctg atcacggctc
     2641 cgggagacct gaacgttgac tcggcggtgg ccggtgcgca gcgactggcg cgctgggagg
     2701 acgaggattt cgtggcgcag gtctccgccg tggagccgta cgagatcggc ccggcggatg
     2761 gacctgtcat cgcgctgatc gacttcgggc tcaaggtgaa catttgccgc cagctcgttg
     2821 cccgcggcgc tcgagtccga gtgttgccac acacgacgtc cgctgcgacc gcactggcgc
     2881 ctgatgtgcg cggcctcgta ttttcaccgg gtcccggcga tccggcgcgg ctccacgccc
     2941 cggttcgcct cgcgcaggct gcgatcgacg atgggcggcc gctcctgggg atctgcctcg
     3001 ggcatcagat cgtgggccgg gccgccggcg cggagacccg gcggctgcgc ttcggccacc
     3061 acggcgcgaa ccatccggtg aaggacgttg cgaccggcaa ggtccaggtg acggcccaga
     3121 accacgaggt cgaggtggtc ggtgaaacgc tgccgctgca gagcggtttc gaggtcagcc
     3181 agcgcaacct gaacgacggc tcggtcgagg gtctgcgcca cgaacacaag ccgattgaga
     3241 cagtccagta ccaccccgag ggttcgcccg gtccactcga cgccctcgac gtattcga
//
LOCUS       DATLCO010000016         4241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2987430, whole genome shotgun sequence.
ACCESSION   DATLCO010000016 DATLCO010000000
VERSION     DATLCO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987430"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VMZ33_00390"
     CDS             complement(<1..697)
                     /locus_tag="VMZ33_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR15715.1"
                     /translation="MRNGAPKILVVDDNPPTRYSTGRVLRAARFEVIEAATGGEALQA
                     TSQNPDLVILDVNLPDISGFEVCRRLRANPATARTPVVHLSATFVDADAKVQGLDAGA
                     DGYLTHPVEPPVLIATVNAFLRARHAEEGMRSSEAKFKAVFENAASGIALLTDGMVFL
                     EVNPAIASSLGRDRADIEGRHLALFLPSGHAQLLSRIDEGLAATGLWRGAFPILDANG
                     REVELEWSVSIHSD"
     gene            complement(690..1583)
                     /locus_tag="VMZ33_00395"
     CDS             complement(690..1583)
                     /locus_tag="VMZ33_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR15716.1"
                     /translation="MSDREDILSRQVTAYAEEVAALRQELAETNQGVVALYAELDDNA
                     AKLKEASELKSRFLSYMSHEFRTPLGAIRSIARILVDRLDGPLTDEQARQVRFIQDAG
                     AELTNMVDDLLDLAKVEAGRVTISPAWFEMVDLFSALRGMFKPILSNDAVSLIFEAPH
                     DVPPVFTDDKKLSQVLRNFISNALKFTSEGEVRVTATLSDSGLVTFAVSDTGIGIAAE
                     DLGAIFQDFVQVDAPIQKKLRGTGLGLSLSKKLAGMLGGDVHVTSEVGKGSIFSVTIP
                     LSLPGHVTAARPQGSEQPRHA"
     gene            complement(1580..2587)
                     /locus_tag="VMZ33_00400"
     CDS             complement(1580..2587)
                     /locus_tag="VMZ33_00400"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR15717.1"
                     /translation="MPTPSWVLAIEDVSQVGQARRLATRLAEQAGFPEEQCGQAALVA
                     TELSTNLLKYGTQGKLMLQPADWNGDALLQVLAIDHGPGIADVQRCLQDGVSTGGTPG
                     TGLGAVQRLSQVFDIHSTVGKGTVVFAACGRRPARPRKASSFTWTALSTPAPGETVCG
                     DTWAVRETDGGLDVMVADGLGHGPLAFEAAEMAARAFEARPATTVEYLERAHRALQGS
                     RGAALAMARVDPSGAVAYAGLGNISGTIAVDGKGRGLASQNGTVGLQARRLQQHTYEL
                     PANALLTMHSDGLSNRWSLDNYVGLATRHPAVIAGVLYRDMLRGRDDATIVVIRRGTL
                     A"
     gene            complement(2595..2975)
                     /locus_tag="VMZ33_00405"
     CDS             complement(2595..2975)
                     /locus_tag="VMZ33_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010883997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HUR15718.1"
                     /translation="MPIRTEQDIVLARQGVRKLSQEIGLGIVDQTKMVTAASELARNT
                     LVYGRGGEMRCELLLEGLRHGLRVHFVDEGPGIPDLGQAMTDGWTSGTGMGLGLSGAK
                     RLVNEFEIDTAVGRGTRVTITRWK"
     gene            complement(3008..3370)
                     /locus_tag="VMZ33_00410"
     CDS             complement(3008..3370)
                     /locus_tag="VMZ33_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HUR15719.1"
                     /translation="MERIAILRMGEFLLVTIQVDMHDQLALTLQHDLTDSIQRHQAKG
                     VLIDISALEMVDSFIGRMIANISAMSRMLDAETMLVGMRPAVAITLVELGLDLPGVHT
                     ALDVERGMARLRARLSDN"
     gene            complement(3374..>4241)
                     /locus_tag="VMZ33_00415"
     CDS             complement(3374..>4241)
                     /locus_tag="VMZ33_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HUR15720.1"
                     /translation="PRGPDMAKSRPSIAGLLRDDPKKILTEWVADLRASSMDSRIDIG
                     MIEAQATEFLQLMGKAGQESLDPSTAPWQDTRAFLEGISKSRAAQGLSSMETATFVFS
                     LKRALFARLQAAAGTDAETLARETWVITELIDKLGLHTVNAFQKAREAVIARQQEELL
                     ELSTPVVKLWDGVLALPMIGTLDSSRTQVVMESLLQRIVETGAEIAIIDITGVPTVDT
                     LVAQHLLKTVTALRLMGADCIICGIRPQIAQTIVHLGIELQGITTKATLADALALALK
                     RSGLVVTKAPHA"
BASE COUNT          686 a         1421 c         1390 g          744 t
ORIGIN      
        1 cgtcggagtg aatcgacacg ctccattcca gttccacttc acggccattg gcgtcgagga
       61 tcggaaacgc gccgcgccac agacctgtcg ccgcgaggcc ttcgtcgatg cgcgacagca
      121 gctgcgcgtg gccggacggc aaaaacaggg cgagatggcg cccctcgatg tccgcgcggt
      181 cgcgtccgag cgacgaggcg atcgcggggt tcacctcgag gaagaccatg ccgtccgtca
      241 gcagggcgat accgctggcc gcgttctcga acaccgcctt gaatttggct tcgctggagc
      301 gcatcccctc ctcggcgtgg cgggcgcgga ggaaggcatt gaccgtcgca atcagcaccg
      361 gcggctcgac cggatgagtc aggtagccgt ccgctccggc gtcgagccct tgcaccttcg
      421 cgtcggcgtc gacgaacgtg gcggagagat ggacgaccgg cgtccgtgcg gtcgccgggt
      481 tggcgcggag ccgccggcag acttcgaagc cgctgatgtc gggaaggttg acgtcgagaa
      541 tcaccaggtc cggattctga gaagttgcct ggagtgcctc gcccccggtc gcggcttcga
      601 tcacctcgaa gcgggcggcg cgcagaaccc gcccggtcga atatcgcgtc ggcgggttgt
      661 cgtcgacgac caggatcttc ggcgccccgt tacgcatgcc gcggctgctc cgaaccctgc
      721 gggcgggcgg cggtgacgtg tcccggcagc gacagcggga tcgttaccga gaagatcgac
      781 cccttgccga cttcgctcgt cacgtgcacg tctccgccga gcatgcccgc cagcttcttg
      841 ctgagcgaca ggcccagacc ggtgccgcgc agctttttct ggatgggcgc gtcgacctgc
      901 acgaagtcct ggaagatcgc gccgagatcc tcggcggcga tgccgatccc ggtgtcgctc
      961 acggcgaagg tgacgaggcc tgaatcggac agggtcgccg tcactcgaac ctcgccctcg
     1021 ctggtgaact tcaaggcatt cgagatgaag ttgcgcagca cctgcgacag cttcttgtcg
     1081 tcggtgaaca ccggcggaac gtcgtggggc gcctcgaaga tcaacgagac cgcgtcgttg
     1141 gacagaatcg gcttgaacat gccgcgcagc gccgagaaca agtcgaccat ctcgaaccac
     1201 gccggcgaaa tcgtcacccg acccgcttcg actttcgcca ggtcgagaag gtcgtcgacc
     1261 atgttggtga gctcggcgcc ggcgtcctgg atgaatcgga cctggcgcgc ctgctcgtcg
     1321 gtcagcggcc catccagacg gtcgaccagg attctcgcga tgctgcgaat ggctccgagc
     1381 ggggttcgaa actcgtggct catgtacgac aggaagcggc tcttgagctc agacgcctcc
     1441 ttcaacttcg ccgcgttgtc gtccagctcg gcgtacagcg cgacgacgcc ctgattcgtc
     1501 tcggcaagtt cctggcggag cgcggcgacc tcctcggcgt acgcggtaac ctgccgggac
     1561 aggatgtcct ctcgatcgct cacgcgaggg tccctcgtct aatcaccacg atggtggcgt
     1621 cgtcacggcc gcgcagcatg tcgcggtaga gcacgccggc aatgactgcc ggatgacggg
     1681 tcgccagccc cacgtagttg tcgagtgacc agcgattact gagcccatca gagtgcatcg
     1741 tcaagagggc gttcgccggc aactcatacg tgtgctgctg gaggcggcga gcttgcagcc
     1801 cgacggtgcc gttctggctc gcaagccctc ggcccttgcc atcgaccgca atcgttccgg
     1861 agatgttccc caacccggcg tacgcgacag cgccagatgg atcgacccgg gccatcgcca
     1921 gagccgcgcc acggctgccc tggagcgccc ggtgagcgcg ttcgagatac tcgacggtgg
     1981 tcgctggacg agcctcgaag gcgcgcgccg ccatttcggc ggcctcgaac gccagcgggc
     2041 cgtgcccgag gccgtcggcg accatgacgt cgagcccacc gtctgtttcg cggacggccc
     2101 aggtgtcgcc acagacggtt tcccccggcg ccggcgtcga caatgcggtc cacgtgaatg
     2161 acgacgcctt gcggggccga gccggccggc gaccgcaggc cgcgaacacc accgtgccct
     2221 tcccgaccgt cgagtgaatg tcgaagacct gtgacaggcg ctggaccgcg ccaaggccgg
     2281 ttcccggtgt gccgccggtc gagacgccgt cctgcaggca gcgttgcacg tcggcgatgc
     2341 cgggaccgtg gtcgatcgcc aggacctgca ggagcgcatc gccattccag tccgcaggct
     2401 gcaacatcag tttgccctgg gtcccgtact tcagcagatt ggtcgagagc tcggtcgcca
     2461 ccagcgccgc ttgcccgcac tgctcttccg gaaagccagc ctgctcggcc agacgggtgg
     2521 cgagacggcg cgcctgtcct acctggctga cgtcttcgat cgcgagaacc cacgacggcg
     2581 tcggcatccg gacgctactt ccagcgagtg atggtcactc tggtgccgcg gccgacagcg
     2641 gtgtcgatct cgaattcgtt gaccagccgc ttggcacctg acagcccaag ccccatgcct
     2701 gtgccggagg tccagccgtc ggtcatcgcc tgcccgaggt cggggatgcc cggcccttca
     2761 tcgacgaagt gcaccctcag gccgtgacga agcccttcca ggagcagctc gcagcgcatc
     2821 tcgccgccgc gtccgtacac gagggtattg cgagccagct cactggccgc ggtgaccatc
     2881 ttggtttgat cgacgatgcc gaggccgatt tcctgggaca gcttgcggac tccctggcgc
     2941 gcgagaacga tgtcctgctc cgtccggatc ggcatcgcac tggtcctgat aaccgccatc
     3001 gttcgatcta attgtcgctg agccgggctc gcagcctggc catgccgcgt tcgacgtcga
     3061 gggcggtgtg gacgccgggc aggtccagcc ccagctccac gagcgtgatg gcgaccgctg
     3121 gccgcatgcc gaccagcatc gtttccgcat ccagcatccg cgacatcgct gagatgttgg
     3181 cgatcatccg gccgatgaag gaatcgacca tctcgagcgc cgagatgtcg atcagcacgc
     3241 ccttcgcctg atggcgctgg atgctgtcgg tgagatcgtg ctgcagtgtc agcgcgagct
     3301 ggtcgtgcat gtccacctgg atggtgacca gcaggaactc ccccatccgg agaatcgcga
     3361 tgcgttccat gcgctaggcg tgtggggcct tggtcaccac caggcccgat cgcttgagcg
     3421 ccagcgccag cgcgtcggcg agcgtcgcct tcgttgtgat gccctgcagc tcgatgccga
     3481 ggtggacgat cgtttgggcg atttgcgggc ggatgccgca gatgatgcag tcggccccca
     3541 tcaggcgcag cgcggtgacg gtcttgagga gatgctgcgc cacgagggta tcgactgtcg
     3601 gcacgccggt gatgtcgatg attgcaatct ctgcgccggt ttcgacgatc cgctggagca
     3661 atgactccat gacgacctgc gtgcgcgagc tgtcgagagt gccgatcatc ggtagcgcca
     3721 gcacgccgtc ccacagcttc acgacgggcg tcgacagttc cagcagctcc tcctgctgtc
     3781 gtgcgatcac cgcctcgcgc gccttctgga acgcgttgac cgtgtgcagg cccagcttgt
     3841 cgatgagctc ggtgatgacc catgtctcac gcgccagcgt ctcggcgtcg gtgcccgcag
     3901 ccgcctgcag gcgcgcgaag agcgcccgct tcagcgagaa gacgaacgtg gccgtctcca
     3961 tcgacgatag gccctgtgcc gcgcggctct tcgagatgcc ctcgagaaaa gcccgggtgt
     4021 cctgccacgg agcggtcgag ggatcgagac tttcctgacc ggccttcccc atcagctgga
     4081 gaaactcggt cgcctgcgcc tcgatcatcc cgatgtcgat ccgcgagtcc atcgacgagg
     4141 cgcgaaggtc cgcgacccac tcggtgagaa tcttcttcgg gtcgtctctc agcagtcctg
     4201 cgatgctcgg tcggctcttt gccatgtcgg gtcctcgtgg g
//
LOCUS       DATLCO010000017        14404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_12193, whole genome shotgun sequence.
ACCESSION   DATLCO010000017 DATLCO010000000
VERSION     DATLCO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12193"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VMZ33_00420"
     CDS             complement(<1..525)
                     /locus_tag="VMZ33_00420"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR15721.1"
                     /translation="MPTKGKPGPLAQQALDLSGTAAHAPLAARMRPQTLDAFVGQEHV
                     IGERGALRRMIDRGHLTSMVLWGPPGSGKTTLARLLAESVDAPFATLSAVMSGVADLR
                     AAIARAREWIESGGRSTVLFIDEIHRFNKSQQDALLPQVEDGTITLIGATTENPYFEV
                     NSALLSRLRVFRLEA"
     gene            677..1876
                     /locus_tag="VMZ33_00425"
     CDS             677..1876
                     /locus_tag="VMZ33_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HUR15722.1"
                     /translation="MTTVNLRIPGPTPLPDAVREAGSNQMVNHRGPEFRDLIMRVTQR
                     MQRAFRTKNDLLILTCSGTGALEAVVVNHLSPGDPVLAVSIGSFGDRFAKIASTYGAH
                     VTKLDFEWGRAAEPSKVAEALRAMADQGRSARAILLTHNETSTGVENPLRDLAAAVRE
                     TSPDTLILVDAISGLGAVPFDADAWGLDVVATGSQKSWMAPPGLAMVSVSERAWVASE
                     RAAMPRFYFDLAKAKKLLATGETPWTPAVGVLFALDVALDILESEGDEHIFARHAACA
                     AAARAGLMALGYSLFAEPKHASKTVTSVWMRDGIEWAALNKDMRARGLVIAGGQDRLA
                     GQIMRIGHLGDVDLEDVIDAIRVVGESTAALGAEVDVEGAVAAARSAGAGGAGKVAQP
                     LAATATA"
     gene            1873..2859
                     /locus_tag="VMZ33_00430"
     CDS             1873..2859
                     /locus_tag="VMZ33_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HUR15723.1"
                     /translation="MRVLVAEPVGEPGLALLRGNHEVEILLGLSRDRLLAELPRFEGL
                     IVRSGLKVDAEAIAAGTRLLVIGRAGVGTDNIDLAAATDAGIAVVNAPNANTIAAAEH
                     TLALILSLARRVPAADASLRNGEWRRADFVGSELSGKTLGIVGLGRIGLAVADRARAF
                     GMRLLGSDPIVTRDASLLHGVELSDVDDLLAASDIVTLHVPLNDRTRGMLDAAGLSRM
                     KRGALLVNVARGGLVDESALAEALSVGRLGGAALDVYGNEPPLGSPILTAPNTVLTPH
                     LGASTTEAQERVSIETCEQVADILEGRDAPFVVNAPLLNRRPHPEPAISGAG"
     gene            2860..3711
                     /locus_tag="VMZ33_00435"
     CDS             2860..3711
                     /locus_tag="VMZ33_00435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUR15724.1"
                     /translation="MPEQKLLIPPTVAASLVLVRHGESTWIAEGRFQGRQNPPLSTLG
                     QRQAELVGLRLAHRDERTPLPIPAGPPIGIWHSPLSRVAATAKAIAEAQSGRVPRHAL
                     EQLTEIAQGEWEGQPQAVVKTRWARELNAWRLTPTRSQAPGGERLLDAAARVRGGLGE
                     ILAALTPPASDADARSSDAAGVIASLRHDPVPGYPSSEPEGPPEPWAIVVAHDGIFRL
                     LVLQLLRMPLTRFWSLPFNLCAITVVTIHDGVAALRAHNLSDHLAPLADEERAAQEAR
                     GERRGAL"
     gene            complement(3777..6197)
                     /locus_tag="VMZ33_00440"
     CDS             complement(3777..6197)
                     /locus_tag="VMZ33_00440"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HUR15725.1"
                     /translation="MSAITTSRHKDTRGLDVRAEEREELADMPLTPREGWLSVISLAA
                     MMMVVGIAIDDARWAGLASNSSTSQTGFLPICGVFSVLVGVALAKGKVGRYSGHLIGS
                     TIGALFVLNAAAASISNAPSIEGRLRALNESVTLFVDQVIVEGARSYETSIFLILLGA
                     LVWGAGQFSAYAVFRRHKPLPAILLTGFMLLLNVSVTVKDEYVHLIAFMAAALVLLVR
                     LNLLDQTREWRARGMGDVAEISGAFLRNGASFVAIAIIAATTLAANASSAPLARAWQN
                     LDDDLLEVGYSLNRWLGGISGAARGPNVLFTPNQTIRGVWESSSAPVFTAVTSDGEGY
                     RWRGATYDAFDGHTWSQSDRESIIVGAGDDVLGQTPDAIDPEGDARYPVEVTINPIDY
                     GGDVIVAPADPVELAQDVEVQTHGLSGPFAAVKLTAGIQTDVPFTVLSLVREVRGENA
                     LTGNELAVAGTQYPDWVSRYLQIGEESSGDIVAQAAQDIVASLPASKRDPYHIADAVE
                     NYLWRTGGFEYNTDVRGLCPADQPLADCLLTVKQGYCEYFATAMTMMLRELGIPARYV
                     VGYLPGREQQDGSYLVDRSAAHAWVEVYFPGHGWVEFDPTPGNGENGQEPTNFAAGPD
                     RGPAPTRRPLGGGPNELECADPTDIQCQQPGGPPVFPVDPPVTPTPPRNDILPLAIGG
                     GAVLGLLLLALMASLRHIPKTEPELAYRGVTRLATRLGHGPRPAQTAYEFASGLGEVM
                     PLARTDLHLIATAKVEATYGRKDQQGTMLRMLGLAYRRVRLGMLRLVLKKRPRIGHRP
                     RSGKAKRE"
     gene            complement(6194..7465)
                     /locus_tag="VMZ33_00445"
     CDS             complement(6194..7465)
                     /locus_tag="VMZ33_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUR15726.1"
                     /translation="MLRRIQVIALAAVLLIAALSTGAPFLFFLVYLSIVIVGGAYVVT
                     RFGLADLEAGYVLDRLHAQAGDLLRASYTVRNTSRLPKLWLEVHNPTDLPIPLPGQAI
                     SLGPRGERSWSVRVPLSRRGHFRIEPLALRTGDPLGLFESHATVGSHSTVIVYPRVET
                     LPAWRLPPAQMEGSHAQTLRTPHTTPHATGIRPYAPGDSYNRIHWKSSARQGELQVKE
                     FDLEQTADVWIYIDLDARSHTGDGEESTIEYGVRVAAAIAARALVENRNVGMTASGIR
                     TGVLPADRGPRQYQKIMQLLAAVSPNGERPLTTVLIDGVGRLRRGMSAIVVTPSLNRD
                     WVRPLRDLRGRGVETTVVLLDAPAFAAVARRERGISDPEDDVIEGEQRASRAIRHALA
                     EYDLTWHGIKPGEPIAAQLVTASPRPVLTAR"
     gene            complement(7486..8463)
                     /locus_tag="VMZ33_00450"
     CDS             complement(7486..8463)
                     /locus_tag="VMZ33_00450"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUR15727.1"
                     /translation="MASIQESAEKVIANVERVIVGKHQEVRLALVALLCQGHLLIEDV
                     PGTGKTVLAKAIAKSIGCTFRRIQFTPDLLPSDVTGLSIFNQKTQEFEFRPGPIMAQV
                     VLADEINRATPKTQSSLLECMEERQATVDGVTYQMPDPFMVIGTQNPIEYEGTFALPE
                     AQLDRFMLRLQLGYPQPLEEIVILDEQKRVHPIDDLAEVASTEDLRTMQAAVREIYVD
                     SAISDYIVRLVNATRNHPDVYLGASPRGSLALYRAGQAYAALNARDYVIPDDIKALAV
                     PAIAHRLIVKTAATVRDVDAVLVVRELLDSVPVENRAVTQSSRQTVKPA"
     gene            complement(8601..10109)
                     /locus_tag="VMZ33_00455"
     CDS             complement(8601..10109)
                     /locus_tag="VMZ33_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HUR15728.1"
                     /translation="MGRLPWRRPGRGRKGPARVAASLPRLSLALALGYSALAGGLVYW
                     QVFQANELTTDPGNPLVIAASRQALRGTIYDRNGIVLAHNAAGGRREYPYLAAAPVVG
                     YRSTLFGTAGLERTYDAALTGLVSRRPGDELLRKFRDTPYNPSDVYTSLDINLQQVAA
                     DLLANERGAIVAIEPSTGRILALVSSPTYNPNRITDPDTARSYMADITVREDSPLLNR
                     ASQGLYVPGSVFKIVTAIAGIGSGAVNGSTTFEDQPAEYESGFLVEGFRIHDFPRRVQ
                     LDHPLDFYEATEISSNIWYAHAGLEIGAGTLDEFAARLGFGQRVPFELPTSPSQITGG
                     EGPQDSFRDQVELANAAYGQGEVLVTPLQMALVASTIANDGLLMRPKLVDELRAEDGT
                     VSTLSPDAMSQVLGFSEAQVIGEAMRGAVEGPFGENLAGAAKVPGIPTAGKSGTAELG
                     DDAAPHSWFIGYAPADNPQIAIAVIVEGGGAGSQRAVPMGGDILAAFLLGGE"
     gene            complement(10121..11560)
                     /locus_tag="VMZ33_00460"
     CDS             complement(10121..11560)
                     /locus_tag="VMZ33_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HUR15729.1"
                     /translation="MTVVAQAMYRVGIRPHFRRREAGLLLLASAALLVGKASLESFRQ
                     GGFQIGDAQLLVTFLGLAWATHVAFVITGRRMDEVLYPVAVLLSGISLLLMERLPQDL
                     VVQDLLGSELTLGALQLVWTSIGFVILAATAILVRSDGWLRRYKYTWAAVGIGLLLLV
                     FLFGDVTGGARLTLRIGPFGGQPSELLKVILVVFLAGYLAENRSLLAQSSTRIGPFSV
                     PPLPYLAPMLAMWGLALAIVIVQRDLGAALLYFVVFLALLYVATRRASYVSLGLILFI
                     AGGAVLYLLFPHVQDRIDIWIDPFQDPQGAGYQIIRAMYAYGRGGVLGTGLGAGLPQV
                     GNVPSIPAVHTDFVFTALAEEMGMLGALAVLGLYALIAQRGFRIAATAADDFRALLAT
                     GLTLVIVVQAAIICGGNLRVVPLTGITLPLISYGGSSILVNGAVIGLLLALSDRGVEP
                     PPPGGRKGIVGRFGGRARAVGGRVEEALS"
     gene            complement(11598..12038)
                     /locus_tag="VMZ33_00465"
     CDS             complement(11598..12038)
                     /locus_tag="VMZ33_00465"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUR15730.1"
                     /translation="MDAFTLTLWLLRIGFVVLIYLFLFMVARALWSDLRAGVAGVGRP
                     IGRLLVVASEEGRPPTGTSVTLDAVNSIGRDVNNSIVVDDTFVSAEHALLTFRGRAWY
                     IEDRGSTNGTWLNGRRVEGLLPLGYGDEIQVGQVRLRLERSRAE"
     gene            complement(12053..12850)
                     /locus_tag="VMZ33_00470"
     CDS             complement(12053..12850)
                     /locus_tag="VMZ33_00470"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HUR15731.1"
                     /translation="MAGPLTAVERFFERLFERPAARLFQARVETVQIQRALERVMESE
                     RVVQARRSYAPSVFRVLLNAADMATLPSDRVSLPAELGEGLRQYARARSYTMLARPRV
                     EIEVSAHVPEGDIRAFADKPVVPAAESGSTDGRRSPADFVAAHPAPTFLPEAGQQTQV
                     FAAAQPSTPRAVLVVRSSTQGIGRFPVSAGTTRIGRAPENDIVLIDDRVSRRHGQIAT
                     RFGMLVYTDLGSTNGSFLNGSPVTEIALGPTDLLQVGSSTITIEPTL"
     gene            12970..14100
                     /locus_tag="VMZ33_00475"
     CDS             12970..14100
                     /locus_tag="VMZ33_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15732.1"
                     /translation="MAGFELFCERCGQRYGGQEEATRRPLGQRLLNAVGFGATATAAP
                     EEPLLRFCLACRGYSCPSCWNDEAGFCQTCVPMPEPEFVPDPVVLPELPSLPSLPSLP
                     LLPTVPDPVFVPQPIFAAVDEMVEVSPLAATPEVVVAAPDVFAAASGATPEPAPAPVS
                     VDVPAPGAERVPEYPWEFALDAYADTEQPFVAEAPAADDPAWAMLDAELELEPQPEPQ
                     PVAAAAEPTAATPEPEPVAIEVAAAVAAEVAAEPTVELPGEPLPGPVAAESWTDVPET
                     PALIEEVPPAPPVFIPLPPLGPIITPPRPAPSSLPSIAFDMPDTPPAFLLALPSASAP
                     ARPALPAGLFDGQRPNVRPCPNCDLPVSAAARFCRRCGSAQA"
     gene            complement(14120..>14404)
                     /locus_tag="VMZ33_00480"
     CDS             complement(14120..>14404)
                     /locus_tag="VMZ33_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HUR15733.1"
                     /translation="MLVPAKSPSLRAAVPPAIAAGVGIGLLTMLFSALTPWLIGGLLA
                     FGVIATVFGALIWLSLSYQILLFGGAWARLRRDHEIRRGEQKAAGAPFAS"
BASE COUNT         2448 a         4734 c         4923 g         2299 t
ORIGIN      
        1 cgcctcgagg cggaacacgc gcagccggga gagaagcgcc gaattgacct cgaagtacgg
       61 gttctctgtc gtcgccccga tcaaggtgat cgtcccatct tccacctggg gaagaagagc
      121 gtcctgctgg ctcttgttga agcggtgtat ctcgtcgatg aagaggacgg tcgatcgacc
      181 accggactcg atccactcgc gcgcccgagc gatagcggcg cggagatcag ccacgcccga
      241 catgaccgcc gacagcgtcg cgaatggagc gtcgaccgat tcggccaaca agcgggcaag
      301 ggttgtcttg ccacttcccg gaggtcccca caggaccatc gaggtgaggt ggccgcggtc
      361 gatcatccgg cgcaaggcgc cgcgttcgcc gatgacgtgc tcctggccga cgaacgcgtc
      421 gagggtctga ggccgcatgc gggcggcaag cggtgcgtgt gctgcagtgc cagacaggtc
      481 tagcgcctgc tgcgcgagcg ggccgggctt gcctttggtg ggcatatcgg gagtatgcgc
      541 ggcagggagg atgctggcat cgcggtgcgc agtcgacgcg tagcatcgac cggccggcga
      601 tgcgaagtca gcatgggtcc cggagatgtt cctcggctac catcggacca acaagcgcga
      661 ccgaggggag gccaccgtga cgaccgtaaa tctgcgcatt ccggggccca ctcccctgcc
      721 cgacgcggtc cgcgaggcag gctcgaacca gatggtcaac catcgcgggc cggagttccg
      781 cgacctgatc atgcgcgtga cccagcgcat gcagcgcgcc ttccgaacga agaacgacct
      841 cctgatcctc acctgctccg gcactggcgc gctggaggcc gtcgtcgtca accacctctc
      901 tccgggggac ccggtgctgg cagtgagcat cggctcattc ggagaccgct tcgcaaagat
      961 tgcgtcgacg tacggcgcgc acgtcacgaa gctggatttc gaatggggtc gcgctgccga
     1021 accctcgaag gtggctgagg cgctgcgcgc gatggcggac cagggccgct ctgcgcgcgc
     1081 aatcctgctt acgcataacg agacatcaac tggggttgag aaccccctcc gcgacctcgc
     1141 cgcggctgta cgcgagacat cccccgacac gctcatcctc gtcgacgcca tcagtggcct
     1201 cggcgcggtg ccattcgacg ctgacgcatg gggcctcgac gtcgtcgcca caggctcgca
     1261 gaagtcgtgg atggcgcctc cgggcctggc catggtcagt gtcagcgaac gtgcctgggt
     1321 cgcatcggag cgagcagcga tgccgcgctt ctacttcgac ctggcaaagg ccaagaagct
     1381 gctggctacg ggcgagacgc cgtggacgcc ggctgttggc gtcctcttcg ccttggacgt
     1441 cgcgctggat attctcgagt cagaaggcga cgagcacata tttgcccggc acgccgcgtg
     1501 cgctgcggcc gctcgtgcag gcctgatggc cctcggctat tccctgttcg cagagcccaa
     1561 gcacgcttcg aaaaccgtca catcggtgtg gatgcgcgac ggcatcgaat gggcggcgct
     1621 caacaaggac atgcgggcgc ggggcctggt catcgccggc ggacaggaca ggctggccgg
     1681 gcagatcatg cgcatcggcc acttgggcga cgtggatctc gaggacgtca tcgacgccat
     1741 tcgtgtggtg ggcgagtcga cggccgccct gggcgccgag gttgacgtcg agggcgcagt
     1801 ggcagctgct cgttctgcgg gggcaggtgg cgccggcaag gtggctcaac cgctggccgc
     1861 gaccgctacc gcgtgagggt cctggtagcc gaacccgttg gcgaaccggg gctcgccctg
     1921 ctgcggggga accacgaagt cgaaatcttg cttgggctga gccgcgaccg gctgctcgcc
     1981 gagctgccac gcttcgaagg gctgatcgtc cgtagcggct tgaaggtcga cgccgaggcg
     2041 atcgccgccg gcacgcgatt gctggtgatc ggtcgcgcag gcgttgggac ggacaacatc
     2101 gatcttgccg cggccaccga tgctgggatc gccgtcgtga acgcgccgaa cgcgaacacg
     2161 atcgccgccg ccgagcacac cctcgcattg atcctttcgc tggcgaggcg cgtccccgct
     2221 gccgacgcgt cgcttcgcaa cggggagtgg cgccgagcag acttcgtcgg cagcgagctt
     2281 agcggcaaga ccttgggcat cgttggcttg ggcaggatcg gtttggccgt ggctgatcga
     2341 gcgagagctt tcggcatgcg gcttttgggg tcggatccca tcgtgacccg cgacgcgtcc
     2401 ctgctgcacg gcgtggagct gagcgacgtg gacgacctcc tggcagcctc cgacatcgtg
     2461 acgctgcacg tgccgctcaa cgacaggacg cgcggcatgc tcgacgcggc agggctctcg
     2521 cggatgaagc ggggtgcgct tctggtcaac gtggcacgcg ggggcctcgt cgatgagtcg
     2581 gcactggccg aagcgctcag cgttgggcgt ctcggtggcg cggcgctcga cgtgtacggc
     2641 aatgagccgc cactgggatc gccaatcctc accgctccga ataccgtgct gacaccgcac
     2701 ctcggcgcat cgacgactga ggcccaggag cgggtttcca tcgagacctg cgaacaggtc
     2761 gccgacattc tcgagggccg cgacgccccg ttcgtggtaa atgcgccgct cctcaaccgc
     2821 cgtccacatc cggaacctgc gatcagcggc gcgggatgag tgcctgagca aaagctcctg
     2881 atcccgccca cggttgccgc gagcctcgtc cttgtccgcc acggcgaatc gacgtggatt
     2941 gcggagggtc gattccaggg acgccagaac ccgcccctat cgacgctcgg gcagcgtcag
     3001 gcagagcttg tcggactgcg cctggcgcat cgcgacgagc ggaccccgct gcccattcct
     3061 gctggtcctc cgatcggcat ctggcactcc ccgctgagcc gggtagcagc gacggcgaaa
     3121 gccatcgccg aagctcaatc cggacgggtt ccgcggcacg cgctcgagca gctgaccgag
     3181 attgcccagg gcgaatggga gggacagccg caggcagtcg tgaagacacg ctgggcgcgc
     3241 gagctcaacg cgtggcgact gacgccgacg cgctcccagg caccgggcgg cgagcgactc
     3301 ctcgacgcag ccgcgcgcgt ccgcggaggg ctcggcgaga ttctggcggc gctgacgccg
     3361 cctgccagtg acgccgatgc gcgttcatcg gacgcggccg gggtgatcgc gtccttgcgc
     3421 cacgatcccg tgccgggcta tccaagtagc gagccagagg gcccaccgga accgtgggca
     3481 atcgtcgtcg cgcacgacgg catcttccga ctgttggtgc tgcagctact ccgaatgccg
     3541 ctgactcggt tctggtcgct gccgttcaac ctgtgcgcga tcaccgtcgt gacaatccac
     3601 gacggagtgg ccgcgctacg cgcccacaat ctgagtgacc acctggcgcc tctggctgac
     3661 gaggaacgag ccgcgcagga agcacgcggc gagcgtcgag gggccctcta gcaaggagcg
     3721 cgatgggctc gcgccagcaa ggccagcgcg cgacgctccc gcagctgcgc ctcttgttac
     3781 tcgcgcttcg ccttgccaga gcgcggccga tggccgattc tcgggcgctt cttcagtacc
     3841 aatctgagca tgcccagtcg gactcgccgg tatgccagcc cgagcatccg gagcatcgtg
     3901 ccctgctggt ccttccggcc atatgtcgcc tcgaccttgg cggtcgcaat gagatggagg
     3961 tcggttcggg caagtggcat gacctcgcca agcccgctgg cgaactcgta cgccgtctgg
     4021 gctggccgag gaccatggcc gagcctcgtt gcgagacgcg tcactccgcg ataggcaagc
     4081 tcaggctccg tctttggaat gtgcctcagg ctcgccatca gcgcgagcaa cagcaatcca
     4141 agaaccgcac caccgcctat agcgagaggc aagatgtcat tgcgcggcgg agtcggggtc
     4201 acgggcggat ccaccgggaa caccggtggg ccgccgggct gctggcactg aatgtcggtc
     4261 gggtcggcgc actcgagctc gttcggacct ccgccaagtg ggcgacgcgt cggcgccggg
     4321 ccgcggtccg ggccggccgc gaagttggtc ggttcctgcc cgttttcgcc attgcccggc
     4381 gttggatcga actccaccca gccgtggccg ggaaaataca cctctaccca cgcatgtgcc
     4441 gcgctgcggt ctacgaggta cgagccgtcc tgctgttcgc ggccgggcag atagccaaca
     4501 acgtaccggg ctggaattcc aagctcgcgc agcatcatcg tcatcgccgt cgcgaagtac
     4561 tcgcaatagc cctgcttgac cgtgagcagg cagtcggcca gtggttggtc ggccgggcac
     4621 aggccacgaa cgtcggtgtt gtactcgaag ccgcccgtgc gccagaggta gttctcgacc
     4681 gcgtcggcaa tgtggtacgg atcgcgcttg ctcgccggaa ggctggccac gatgtcctgt
     4741 gccgcctgcg cgacgatatc gcctgacgac tcctcgccaa tctgcagata ccgcgacacc
     4801 caatctggat attgggtgcc cgccactgca agctcattgc ccgtgagcgc gttctcccca
     4861 cggacctcac ggacgagcga cagcaccgtg aacggcacgt cggtctggat acccgccgtc
     4921 agcttcaccg cggcgaacgg ccccgacagc ccgtgggtct gaacctcgac gtcctgggca
     4981 agctcaacgg gatcggccgg tgccacgatc acatcgcccc catagtcaat cggattgatc
     5041 gtcacctcga cggggtagcg cgcgtcgccc tcgggatcaa tggcatcggg cgtctggccc
     5101 agaacgtcgt cgccggctcc gacgatgatc gattcgcggt ccgattgcga ccaggtgtgg
     5161 ccatcgaatg cgtcataggt agcgccgcgc cagcgataac cctcgccatc actggtgaca
     5221 gccgtgaaca cgggggccga agaggattcc cagacgccgc ggatggtctg gttgggtgtg
     5281 aacagaacgt ttgggccgcg cgccgcaccg ctgatgccac ccagccaacg gttgagcgag
     5341 tagcccacct cgaggaggtc gtcgtcgagg ttctgccacg ctctggcaag gggcgcggag
     5401 ctcgcgttcg cggcgagcgt cgtggccgcg atgatggcta tggccacgaa cgaggcgccg
     5461 ttgcgcagga aagcaccgga gatctcggcg acgtcgccca tgccccgcgc tcgccattcg
     5521 cgcgtctgat cgagcaggtt gaggcggacc agcagaacaa gcgctgcggc catgaacgcg
     5581 atgagatgga cgtactcgtc cttcaccgtc accgagacat tgagcagcag catgaagccg
     5641 gtcaacagaa tggccggcag cggcttgtga cggcggaaga cggcgtaagc gctgaactgg
     5701 ccagcgcccc acacgagggc gcccaggagg atcaggaaga tggatgtctc gtacgagcgg
     5761 gcaccctcga cgatgacctg gtcgacgaac agcgttacag actcgttcag cgcacgcagg
     5821 cgaccctcga tggatggagc gttcgagatc gatgcagccg ccgcgttcaa aacgaaaaga
     5881 gcgccgatcg tcgaaccgat caggtggccg ctgtaacgac caaccttgcc ctttgcgagc
     5941 gctaccccga caaggacgga gaagacgccg cagatcggca ggaagcccgt ttgggacgtc
     6001 gacgagttcg atgcgagtcc agcccagcgg gcatcgtcaa tcgcgatgcc gacgaccatc
     6061 atcatggccg cgaggctgat aacgctgagc cagccctcgc gaggggtcag cggcatgtcc
     6121 gccagctctt cgcgctcctc ggcgcgaacg tcgagcccgc gggtgtcctt gtggcgggac
     6181 gtggtgatgg cgctcatcgg gctgtcagga ccggtcgtgg agatgcggtg acgagctgtg
     6241 cagcgatcgg ctcgccgggc ttgataccgt gccaggtcaa gtcgtactcg gccagggcgt
     6301 ggcggatcgc tcggctggct cgctgctcgc cctcgatgac gtcgtcctcc gggtctgaga
     6361 tgccacgctc acgacgggcg acggcggcga acgcaggggc gtccagcagg acgacagtcg
     6421 tttcgacgcc gcggccgcgc aggtcgcgca gcgggcgcac ccaatcgcgg ttgagcgagg
     6481 gtgtgacgac gatggcgctc atgccccgtc ggagccggcc aactccatcg atgagaaccg
     6541 tcgtcagcgg cctctcacca ttgggcgaca cggccgcgag cagctgcatg atcttctggt
     6601 attgccgcgg gccgcggtcg gctgggagga cccctgtccg gatgcctgac gcggtcatgc
     6661 cgacgttgcg gttctccacg agtgctcgcg ccgcaatggc cgccgcgaca cggacgccgt
     6721 actcgatcgt cgactcctcg ccgtcacctg tatgcgaccg cgcgtccaaa tcgatgtaga
     6781 tccacacatc cgcggtctgc tccaggtcaa actccttcac ctgcagctcg ccttgacggg
     6841 cactgctctt ccagtgaatg cggttatacg agtcgccggg cgcatacggt cgaatgccgg
     6901 ttgcgtgcgg ggtcgtgtgc ggcgtgcgca gggtctgggc atgcgacccc tccatttgcg
     6961 ccggtggcag ccgccaggcg ggcagggttt cgacccgggg atagacgatc accgtcgagt
     7021 ggctgcctac ggttgcgtgc gactcgaaca ggcccaacgg atctcccgtc cgcagcgcca
     7081 gcggctcgat acggaagtgg ccacgccgtg agagcggaac gcggacggac caggatcgct
     7141 cgccacgtgg gccgagggaa atcgcctgtc cggggagcgg gatcggcagg tcagtggggt
     7201 tgtgcacctc cagccacagc ttcggcaatc ggctggtatt ccggacggtg tagctggctc
     7261 gcaacaggtc gccggcctgg gcgtggaggc ggtcaaggac gtagccggcc tcgagatcgg
     7321 ccaggccaaa gcgcgtaacg acatatgcgc cgccgacgat gacgatcgag aggtacacga
     7381 ggaagaacag gaatggggcg ccggtagaca gtgctgcgat aagcaggacc gccgcgagag
     7441 cgatgacctg gatgcgccgg agcatctcgt gctggcccgc gatcgctagg ccggcttgac
     7501 cgtctgtcga ctcgactggg ttacggccct gttttcaaca ggaacgctgt cgagcagctc
     7561 gcgaacgacg agcaccgcgt cgacgtcgcg caccgtggcc gccgtcttga cgatgagccg
     7621 atgcgcgatc gcaggcacgg ccagggcctt gatgtcgtcc gggatcacgt agtcgcgcgc
     7681 gttgagcgcg gcatatgcct gacccgcccg gtacagcgcc agcgaaccgc gcggcgacgc
     7741 accgagatat acgtcagggt ggttacgggt cgcattgacg agacggacga tgtagtcgct
     7801 gatggccgag tcgacgtaga tctcacgcac cgcggcctgc atggtgcgca gatcctccgt
     7861 tgaggcgacc tccgcgagat cgtcgatggg atgcacgcgc ttctgctcgt cgaggatgac
     7921 gatctcctcc agtggctgcg ggtagcccag ctgaagcctg agcatgaagc ggtcgagctg
     7981 tgcctctggc agcgcgaagg taccctcgta ctcgatcggg ttctgggtgc cgatcaccat
     8041 gaacggatcg ggcatctggt aggtgacgcc atcgacggtc gcctggcgct cctccatgca
     8101 ctcgagcagc gacgactgcg tcttgggcgt ggcgcggttg atctcgtcag cgaggacgac
     8161 ctgggccatg atcgggcccg gccggaactc gaactcctgg gtcttctgat tgaagatcga
     8221 gaggccggtg acgtccgacg gcagaaggtc cggcgtgaac tggatgcgtc ggaacgtaca
     8281 gccgatgctc ttggcgatcg ccttcgcgag gacggttttg ccggtaccgg gcacgtcctc
     8341 gatgagcagg tggccctggc atagcagcgc gacaagggcg aggcgcacct cctggtgctt
     8401 gcccacgatg acgcgctcta cgttggcgat gaccttctcg gccgattcct ggatcgatgc
     8461 cacagagtga ccccttcggg tgtgacggtt ccctattgcc cctcggccgt ctgggctccg
     8521 gcggaggcgt cggcagatag tacgcccagc actcgaaaac gcccacgctc atgtctagga
     8581 cgctcggcgc tgcccgcgtg ctactccccg ccgagcagga aagcggccaa gatgtcgccg
     8641 cccatcggaa ccgcccgctg cgaccccgcg ccgccgcctt ccacgatcac cgctatggcg
     8701 atctgcgggt tgtcggctgg tgcgtagccg atgaaccatg agtgcggcgc cgcgtcgtca
     8761 cccagttcgg ccgtgccgct cttgccggca gtcgggatgc ccggcacctt cgccgcaccg
     8821 gccaggttct cgccgaaagg cccctccacg gccccccgca tcgcctcacc gatgacctgg
     8881 gcttcggaaa atccgaggac ctggctcatg gcgtcggggc tcaacgtgga gacggtgccg
     8941 tcctccgcgc gcaactcatc tacgagcttc ggccgcatca gcaggccatc gttggcgatt
     9001 gtcgacgcga cgagcgccat ctggagcgga gtgaccagca cctcaccctg gccgtatgcg
     9061 gcgttggcca gctcgacctg gtcacggaag ctgtcctgcg gcccctcacc cccggttatc
     9121 tggctgggtg aggtcggcag ctcgaaaggg acgcgctggc cgaaaccaag acgcgccgcg
     9181 aattcgtcga gcgtgcccgc cccgatctct agcccagcgt gcgcgtacca gatgttgctg
     9241 gaaatctcag tggcctcgta gaagtcaagc gggtgatcga gctgcacacg gcgggggaaa
     9301 tcgtggatgc ggaaaccctc gacgaggaac cccgactcgt actcggcggg ctgatcctcg
     9361 aaggtggttg agccgttgac cgcgcccgag ccgatgccgg cgatcgcggt cacgatcttg
     9421 aacactgagc cgggcacata gaggccctgg gaggcccgat tcaagagcgg cgagtcctcg
     9481 cggacggtta tgtcggccat gtagctgcgg gccgtgtcgg gatcggtgat gcggttgggg
     9541 ttgtaggtgg ggctcgatac cagggccagg atgcgtccgg tagacggctc gatggcgacg
     9601 atcgcgccac gctcgttggc gagcagatcg gcggcgacct gctgcaggtt gatgtcgagc
     9661 gacgtgtaga cgtcggacgg gttgtagggc gtatcgcgga acttgcgcag cagctcgtcg
     9721 ccggggcgcc gtgatacgag gcccgttagc gctgcgtcgt aggttctctc gaggccggca
     9781 gtaccgaaca gcgtgctgcg atagcccacc accggtgcgg ccgcgagata cgggtattcg
     9841 cggcggcccc ccgctgcgtt gtgcgcgaga acgatgccgt tgcggtcgta gatggtgccg
     9901 cgcagcgctt gacgtgatgc agcgatgacg agtggattac ccggatcggt ggtcagctcg
     9961 tttgcctgga acacctgcca gtaaacgagc ccaccggcga gcgccgaata gccgagcgcg
    10021 agtgccagcg acaaccgcgg caagctggcg gcgacgcgcg ccggaccctt gcgcccgcgc
    10081 cccggtcgtc gccacggaag tcttcccatg aggccggccg tcacgagagt gcctcttcaa
    10141 cgcggccgcc aacggcgcgt gctcggccgc cgaaccggcc cacgatcccc ttgcgcccgc
    10201 ccggcggtgg cggctcgaca ccgcggtcac tgagcgccag caggaggccg atcactgccc
    10261 cgttgacgag gatcgacgag ccgccgtagc tgatgagcgg cagcgtgatg ccggtcagcg
    10321 gcaccacccg caggttgccg ccgcagatga tggcggcctg gacgacgatg acgagggtca
    10381 ggccggtggc cagcagtgcc cggaagtcgt cagcggcagt ggctgcgatc cgaaacccgc
    10441 gctgggcgat caacgcgtac aggccgagga ccgcgagggc gccgagcatg cccatctcct
    10501 cggccagcgc ggtgaagacg aagtcagtgt ggaccgctgg gatcgacggg acgttgccga
    10561 cctgcggcag ccccgcaccg aggccggtgc caagaacgcc gccacggcca taggcgtaca
    10621 tcgcgcgaat gatctggtaa ccggctccct gcggatcctg gaaagggtct atccagatgt
    10681 cgatccggtc ctggacgtgg gggaagagca ggtacagcac cgcccctccg gcgatgaaga
    10741 ggatgaggcc gaggctgacg taggacgccc ggcgggtggc cacgtacagg agcgccagga
    10801 agacgacgaa atagagcagc gcagctccga ggtcgcgctg gacgatgacg atcgcgaggg
    10861 ccaggcccca catggcgagc atcggcgcca gatagggcag cggcgggacc gagaacggcc
    10921 caattcgcgt gctgctctgt gccaacagcg accgattctc ggctaggtac ccagccagga
    10981 agacgaccag gattaccttg aggagctccg atggctgccc gccgaatggg ccgatacgga
    11041 gggtcaatcg cgcgccaccg gtgacgtcgc cgaagaggaa cacgagcagc agcagtccga
    11101 tgccgacggc cgcccatgtg tacttgtagc ggcgcagcca gccgtcactg cgaaccagga
    11161 tggcggtcgc tgccaggatc acgaagccga tgctggtcca gacgagctgc aatgcgccga
    11221 gcgtcaactc gctgccgagc aggtcctgga caaccagatc ttgcggcagc cgctccatga
    11281 gcaggagcga tatgcccgac agcagcacgg ccaccgggta gagcacctca tccatgcgtc
    11341 gacccgtgat cacgaacgcc acgtgggtcg cccaggccag tccgaggaac gtgaccagaa
    11401 gctgcgcgtc gccgatctgg aagcctccct gcctgaaaga ctcgaggctc gccttaccga
    11461 cgagcagcgc ggccgacgcc agcagcagca ggccggcctc acgtcgtctg aaatggggcc
    11521 ggatcccaac ccggtacatg gcctgcgcca cgacggtcat gggctggaac tcccctggaa
    11581 gggcatcggc cgaccgatca ctcggcgcgc gagcgctcga ggcgcaaccg gacctgaccg
    11641 acctggatct cgtcgccgta acccagcggc agcaggcctt ccacgcgacg gccgttcagc
    11701 cacgtgccgt tggtgctgcc gcggtcctcg atgtaccagg cgcgacctcg gaatgtgagc
    11761 agcgcatgct cggctgagac gaatgtgtcg tcgacgacga tggagttgtt cacgtcgcgg
    11821 ccgatggagt tgacggcgtc gagcgtgacc gaagtcccgg ttggcgggcg gccctcctcg
    11881 gatgcgacga ccagcagccg gccgatcggc cggccaacac cggccacgcc ggcgcgcagg
    11941 tcgctccaca gcgcccgcgc aaccatgaac aggaacaggt agatgagcac cacgaaaccg
    12001 atgcgcagca gccagagggt caacgtgaac gcgtccatct cggacggcca gcctagagtg
    12061 tcggctcgat cgtgatcgtc gagcttccca cttgcaggag gtcagtcggc ccgagcgcga
    12121 tctctgttac ggggctgccg ttgaggaacg atccattggt gctgcccagg tcggtgtaga
    12181 cgagcatccc aaagcgggtg gcgatctggc cgtggcgccg agatacgcgg tcatcgatga
    12241 ggacgatgtc gttctctggc gcgcgaccga tgcgcgtggt tccggcactc accggaaagc
    12301 ggccgatccc ctgcgtggaa ctgcgaacca caagaacggc gcgtggcgtg ctgggctgcg
    12361 ccgcggcgaa tacctgtgtc tgctgtccgg cctcgggcag gaacgtgggc gcggggtggg
    12421 cagccacaaa gtcggccggt gagcgccttc cgtctgtcga gccactctcc gcggcaggaa
    12481 cgacgggctt gtcggcgaag gcgcggatgt ctccttccgg cacgtgcgca gaaacctcta
    12541 tctccactcg cggccgggcg agcatcgtgt atgagcgggc acgtgcgtat tggcgcagtc
    12601 cttcgccgag ctcggccggt agagaaacgc gatcgctcgg aagcgtggcc atgtccgcgg
    12661 cgttcaggag gactcgaaag accgacggcg catatgaccg acgggcctgc acgacacgct
    12721 ctgattccat aacccgctcg agtgcgcgtt ggatctggac ggtctcgacg cgagcctgaa
    12781 acaagcgcgc cgccggccgt tcgaacagac gctcgaagaa ccgctcaacg gcggtgaggg
    12841 ggcctgccat tcggtcggcg agtctagcag tctgccctgg gccgaacggt gacggggacc
    12901 atcgccctgt cggagcggta ctggtgctcg ggtactcgcg agcgccccgg ccggtcatcc
    12961 ttgccaccaa tggcgggatt cgagctgttc tgcgagcgct gcggacagcg ctatggcggc
    13021 caggaagagg ccacacgacg accgttgggt cagcgtctgc tgaatgcggt cggtttcggc
    13081 gcgaccgcca cggcagcccc ggaggagccc ctcctccgct tctgcctcgc atgccgtgga
    13141 tattcgtgtc cctcgtgctg gaatgacgaa gccgggttct gccagacctg cgtgccaatg
    13201 cccgagccag agttcgtgcc ggaccctgtg gttctccccg agttgccgtc cctgccgtcg
    13261 ctgccgtcgc tgccgttgct gccgacggtg cccgatccgg tgttcgtgcc gcagccgatc
    13321 tttgctgctg tcgatgagat ggtcgaggtc tcgcctctcg ccgctacgcc tgaggtcgtt
    13381 gtcgcggcgc ccgatgtttt cgcagccgct tccggggcaa ccccggagcc tgcgcccgcg
    13441 ccggtcagcg tcgacgtacc cgcgccgggt gccgaaaggg ttccggagta cccgtgggag
    13501 ttcgccctgg acgcctatgc ggatacggaa cagccgttcg ttgccgaggc acctgcggcc
    13561 gacgacccgg cgtgggcgat gctcgacgcc gagcttgagc tcgagcccca gcccgagccc
    13621 cagccagtcg ccgcggccgc tgagccgacg gccgccaccc ccgaaccgga gccggtggcg
    13681 attgaggttg cggccgcggt cgcggctgag gtcgcggcag agccgaccgt cgagctcccg
    13741 ggggagcccc tacccggacc ggtcgccgca gaatcatgga ccgacgtgcc ggaaacaccg
    13801 gccctcatcg aggaggtgcc gcccgcaccg ccggtgttca tacctctacc gccactgggc
    13861 ccgatcatca cgccgccgag gcccgcccct tcgtcactac cgtcgatcgc gttcgacatg
    13921 cccgacacgc cgccggcatt cctgctcgcc ctgccaagcg cgtcagcgcc ggcacgtcca
    13981 gcgctaccgg ctgggctgtt tgacgggcaa cggccgaacg ttcggccctg tcccaactgc
    14041 gatctgccgg tgtctgcggc cgcgcgcttc tgccgacgct gtgggtctgc gcaggcttag
    14101 cggccgacac cagagcggac tagctcgcga acggcgcccc ggcggctttc tgttcgccgc
    14161 gtcgaatttc gtggtctcgt cgtagccgag cccaggcgcc gccgaacagc aggatctggt
    14221 agctcagact gagccagatg agcgcgccga acacggtggc gatgacacca aaggcaagca
    14281 gcccaccgat cagccacggc gtcagggcgc tgaagagcat ggtcagcagt ccgatgccaa
    14341 cgcctgcggc gatcgccggc ggtacggccg cgcgcaggct gggcgacttg gccgggacga
    14401 gcat
//
LOCUS       DATLCO010000018         5094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3738582, whole genome shotgun sequence.
ACCESSION   DATLCO010000018 DATLCO010000000
VERSION     DATLCO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738582"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VMZ33_00485"
     CDS             <1..861
                     /locus_tag="VMZ33_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR15734.1"
                     /translation="TTGLTWRALDDIWAAAGELDVFSAGWLSDHLSDVSRDRGGPAFE
                     AFTSAAALARRVPGKSIGIAVAASTFRHPAVLAKQATMLDIVTGGRFILGVGAGWHPG
                     EHEAFGVPLPDAPARFDRYESQLRVLKALFSADARQSPGVTLDDPHYPLDQATNEPPP
                     LTPGGPPIWLGGQKRRSLDLIGRYASGWPMYGNRAGDLAFFTEMRGKILDALEAAGRD
                     PQAFTFAAQVNAGTETATRREGLEQARALQKAGAHHVIVGIPGTSTADDLRAVAREVA
                     QPLTDEATAP"
     gene            complement(858..1268)
                     /locus_tag="VMZ33_00490"
     CDS             complement(858..1268)
                     /locus_tag="VMZ33_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15735.1"
                     /translation="MRRFLGLVLMGLAAILVIGCSEIGIPVDTPTAPSGIRGVVLLGP
                     TCATGESPGAYDPVPCVTPYVANLIILDSESVVVKRVTSGGDGSFGADLEPGEYVVTP
                     ATGPDTYPIAQPVSVTVLPGIYVDVEINYDTGIR"
     gene            complement(1265..1828)
                     /locus_tag="VMZ33_00495"
     CDS             complement(1265..1828)
                     /locus_tag="VMZ33_00495"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HUR15736.1"
                     /translation="MVATGRGDDGTTGLLFGGRIDKDDPLTEAYGTVDEAVAALGVAR
                     VLLNGAALAELAESVLRYQRELFVCGAELATNPERRDRLVDGTTRVSEEMLAAVEADL
                     AAREAQITMPREFVVPGETEASAALEVARTVLRRAERRIVSLQRAGLIDSAWLVAYIN
                     RLADLLWVLARAAEQAEDAPATTVRTS"
     gene            complement(1855..2235)
                     /locus_tag="VMZ33_00500"
     CDS             complement(1855..2235)
                     /locus_tag="VMZ33_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HUR15737.1"
                     /translation="MTDQISTDTTETTAPTTGATATATATATAPTVDPQARATELAIL
                     DALRAVVDPEIGMNVVELALIRQVILGKDLTEIKMILTTPFCPYAGSMIQQVKEQVET
                     VVENEVKVTLLAERWDPRDAGLVW"
     gene            complement(2265..2579)
                     /locus_tag="VMZ33_00505"
     CDS             complement(2265..2579)
                     /locus_tag="VMZ33_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HUR15738.1"
                     /translation="MTIKWVKVDPLVMNGEPFCYGTRLSVRNLLEMRNNGLTPSQMMA
                     DHPELRRVGIAEAFRFAADNRARYGDFFESDGSMRGPGFPPDEAGGLPEHLRDLSGVV
                     VP"
     gene            complement(2599..3102)
                     /locus_tag="VMZ33_00510"
     CDS             complement(2599..3102)
                     /locus_tag="VMZ33_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15739.1"
                     /translation="MPHPSLGFPPHDIFAGHPAAAARLRASRGRLTKIALQNALTDFP
                     ALADRYDEATLRLFLRDLNQHIEQLAKGLETGNEWFVTGYAEWLVPIFRRRRIPMRDF
                     IAIVLGLKSATATVLTPEENEKADQLFELWVAQLKEHERLPGDHNGNPIVRFFWKGAG
                     IGDDKWV"
     gene            3125..3562
                     /locus_tag="VMZ33_00515"
     CDS             3125..3562
                     /locus_tag="VMZ33_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15740.1"
                     /translation="MSQRTSAYALGAAALSALAYAILAEPFGFSWGLIVVGLIGGAAI
                     GYLLKTGPEPDPADSQSRSDEYPTERLRRRGQYSAVALAMAAWLIGLALAYVLSQVLY
                     QAATTPLTSRLSLPGLWEYAQIALLPRAIALGATVMTAWRSAR"
     gene            3597..3920
                     /locus_tag="VMZ33_00520"
     CDS             3597..3920
                     /locus_tag="VMZ33_00520"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HUR15741.1"
                     /translation="MAKLLIAVVHAQDAAQLVEALRGAELRMTEVLSRGGFLQARNTM
                     LYIGVEDDQVSTAMRLIEENCSSRTEDVPAEPLGGLDANWLPTEVTHGGATVFVVPLD
                     EIRRI"
     gene            complement(3927..4253)
                     /locus_tag="VMZ33_00525"
     CDS             complement(3927..4253)
                     /locus_tag="VMZ33_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15742.1"
                     /translation="MKRSQLISDTRRLIAEGERLQAAPSIHALRVWLKLSDELLSAAW
                     GPMDRYHLSWLMVGRSDAVRGRRMSEEEELAYVRDVASQKTAALRMSVKAIEEQNMPF
                     VGEDRE"
     gene            4279..>5094
                     /locus_tag="VMZ33_00530"
     CDS             4279..>5094
                     /locus_tag="VMZ33_00530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUR15743.1"
                     /translation="MTTATPASPTPTVHASKLRADPSRLNEVFDRLGARVATGALPAA
                     AVAIGDADGEIASKIFPAADKPFTRDSAFFLASVTKPIFATAFMTLVEEGRIDLYQPV
                     EGLLPEFTADKDARKAGVTWWHLLTHTSGVPDVSPEVIRRTRPSATRMTEMTLAAPLV
                     SEPGTRYEYCTATFYLLARAMERVSGLTYARYLQERVLDPLGMESTFDARTSRRPIVA
                     VQGVGVENRLVRFLIVRYLAGTALPGGGLFGTLDDLLRFGAAMIRPVDGRVPIN"
BASE COUNT          861 a         1748 c         1636 g          849 t
ORIGIN      
        1 accaccggcc tcacctggcg ggcgctagac gacatctggg ccgctgccgg tgagctcgac
       61 gtcttttcgg cgggctggct gagcgaccac ctctccgacg tcagtcgcga ccgtggcggc
      121 ccggcgtttg aggccttcac ctcggccgcg gcgcttgccc gccgcgtgcc cgggaagtcg
      181 atcggcatcg ccgtggccgc gagcacgttc cgccatccgg ccgtgctcgc caagcaggcc
      241 acgatgctcg acatcgtgac cggcggccgc tttatcctcg gcgtcggcgc gggctggcac
      301 cccggtgagc acgaggcatt cggcgttcca ctgcctgacg ctcccgcgcg cttcgatcgc
      361 tacgagtcgc agctccgcgt cctcaaggcc cttttcagcg ccgatgctcg ccagtcgccg
      421 ggcgtcacgc tcgacgatcc gcactacccg ttggaccaag ccaccaacga gccaccgcca
      481 ctgacgcccg gcggcccacc gatctggctc ggcgggcaaa aacgccgcag cctcgacctc
      541 atcggccgct acgcgtccgg ctggccgatg tatggcaatc gcgccggcga cctggcgttc
      601 ttcaccgaga tgcgcggcaa gatcctcgac gccctggagg cagccggccg cgatccgcag
      661 gcattcacct tcgccgccca ggtgaacgcc ggcaccgaaa ccgccacccg acgcgagggc
      721 ctcgagcaag cgcgggccct acagaaagcg ggcgctcatc acgtgatcgt cggcattccc
      781 ggcacgtcca ccgccgacga cctcagagcc gtggcccgtg aggtcgccca gccgctcacg
      841 gacgaggcga cggcgcccta gcgaattccc gtgtcgtagt tgatctcgac gtccacgtat
      901 atcccgggca acaccgtcac ggataccggc tgcgcaatcg ggtaagtgtc cggacccgtg
      961 gcaggcgtca ccacgtactc acccggctcg aggtcagcgc cgaaggagcc atcgcccccg
     1021 gaggtgacgc gcttcactac gacgctctcc gagtcgagaa tgatgaggtt cgccacgtac
     1081 ggggtgacgc acggcacggg atcgtaggcg ccgggcgact cgccggtggc gcacgtcgga
     1141 cccaagagga caactccgcg gatgcccgaa ggtgccgtcg gcgtgtcaac cgggataccg
     1201 atctccgaac agccgataac caagatcgcg gccaagccca tcagaacgag acctaggaat
     1261 cgcctcatga cgtgcgcaca gtagtcgccg gcgcgtcttc cgcttgctcg gctgcgcgcg
     1321 caagcaccca cagcagatcg gccagccggt tgatgtacgc caccagccac gccgaatcga
     1381 tcagccctgc acgctgcaac gaaacgatgc gcctctctgc gcgacgcagg acagtgcgtg
     1441 cgacctccag cgccgcagac gcttctgttt caccggggac gacgaactcg cgcggcatgg
     1501 tgatctgcgc ctcgcgcgct gccaggtcgg cctcaacagc ggcaagcatt tcctcggaaa
     1561 cgcgcgtcgt cccatctacc aaccgatcgc gccgctcagg gttcgtggcc agctcggcgc
     1621 cacagacgaa cagctcgcgc tggtagcgga gcactgactc agccagctct gccagcgcgg
     1681 cgccgttcaa caggacacgc gccacgccca gggcagcgac tgcctcgtcg acagtgccat
     1741 atgcctctgt gaggggatcg tccttgtcga tccggccccc gaacaggaga ccggtcgtgc
     1801 cgtcgtcgcc acgtccagta gcgaccacgc tcgcagcaat ggaagagcgg cgggctacca
     1861 caccaggccg gcgtcgcgcg ggtcccagcg ctcagccaac agcgtgacct tgacctcgtt
     1921 ctcgaccacg gtctcgacct gctccttcac ctgctggatc atcgaaccgg cgtacgggca
     1981 gaagggcgta gtcaggatca tcttgatctc agtcaggtcc ttgccgagga tgacctgcct
     2041 gatcagcgcc agctcgacga cgttcatgcc gatctccggg tccaccacgg cgcgcagcgc
     2101 atcgaggatc gccagctccg tggcgcgcgc ttgcggatcg acggtcgggg ccgtcgcggt
     2161 cgctgtcgcg gtggcggtgg cgccggtagt cggagcagtg gtctccgtgg tgtcggtcga
     2221 aatctgatca gtcatgtcgg atatagatca tacgacgaag cgacctaggg gacgaccacg
     2281 cccgataggt cgcgcaggtg ctcgggcagg ccgccggcct catcgggagg gaaaccgggc
     2341 ccgcgcatcg aaccgtccga ctcgaagaag tctccgtacc gcgcgcggtt gtccgcggcg
     2401 aagcggaagg cttcggcgat gccaacgcgc cgcagctcgg ggtggtcggc catcatctgc
     2461 gagggcgtta gcccgttgtt gcgcatctcg agaaggttgc gcaccgacag ccgcgtgccg
     2521 tagcagaaag gttcgccgtt catcacgagc ggatcgacct tgacccactt gatcgtcatc
     2581 gcccttcgtg cccgcgcgtc agacccattt gtcgtcgccg atgcccgcgc ccttccagaa
     2641 gaagcggacg atcgggttgc cgttgtgatc tccgggcaag cgctcgtgct ccttgagctg
     2701 agcgacccag agctcaaaca actggtcggc cttctcgttt tcttcggggg tcagcacggt
     2761 cgctgtggcc gacttcaggc cgaggacgat cgcgatgaaa tctctcatcg ggattcggcg
     2821 gcgccgaaaa atgggcacga gccactcggc atagcccgtg acgaaccact cattgcccgt
     2881 ctcgagaccc ttggcgagtt gctcgatgtg ttggttgaga tcacgcagga acagacggag
     2941 ggtcgcctcg tcgtagcggt cggcgagcgc cggaaagtcg gtcaatgcat tttgaagcgc
     3001 aatcttcgtg aggcgcccgc ggctggctcg gagacgcgcg gccgcggccg gatgtccggc
     3061 gaagatgtcg tgcggcggga agccaagact tggatgcggc atgcggcgac ctagtctatc
     3121 gggtgtgagc cagcgaacct ccgcatatgc cctcggcgct gccgcgctct cggcgctcgc
     3181 ctacgcaatc ctcgctgagc ccttcggctt ctcctgggga ttgatcgtcg tgggcctcat
     3241 cgggggggcc gcaatcggct atttgctgaa gaccggaccg gaacccgacc cggccgactc
     3301 gcagagtcgc tcagatgaat atccaactga gcgactcagg agacgagggc aatattccgc
     3361 ggtcgcgctg gcgatggccg cctggctcat cggcctggcg ctcgcgtacg tgctcagcca
     3421 ggtcctctat caagcggcca cgacgccact cacgtcgagg ctgtcacttc ccggcctgtg
     3481 ggaatacgcg cagattgccc tgctcccgcg agcgatcgcg ctcggggcga cggtgatgac
     3541 cgcgtggcgg agcgcccggt aatcgtgact cccgttcggc gatcattgga cacctgatgg
     3601 ccaagttgct catcgccgtt gtccacgccc aggacgcggc gcagctcgta gaggcgttgc
     3661 gcggtgccga gctgcgcatg accgaggtcc tgtcgcgcgg cggattcctg caggcacgca
     3721 acacgatgct ctacatcggc gtggaggacg atcaggtatc gacagcgatg cgcctgatcg
     3781 aggagaactg ctcctcgcgc actgaagatg tgccggccga gccactcggc gggctcgacg
     3841 ccaattggct accgaccgag gtcactcacg gcggggcgac cgtcttcgtc gtgccactcg
     3901 acgagatccg ccgcatctaa ccggagctac tcgcggtcct cgccgacgaa aggcatgttc
     3961 tgttcctcga tggccttcac gctcatgcgc agcgcggctg tcttctgtga cgcgacgtca
     4021 cgaacgtagg ccagttcctc ctcctcggac atgcgccggc cgcgaacggc gtcgctccgg
     4081 ccaaccatca gccacgacag gtggtagcga tccatcgggc cccacgccgc gctgagaagc
     4141 tcgtccgata gcttcagcca cacgcgcagc gcgtggatgc tcggcgcagc ctgcagccgt
     4201 tcgccctcag cgatcaggcg gcgggtgtcg gagatgagtt gagagcgctt catcggtagc
     4261 ccgattatgg tgagtgtgat gacgacggcg actcccgcct cgccgacgcc gaccgtccac
     4321 gcatcaaaac tgcgcgcaga tccgagtcga ctgaacgagg ttttcgaccg actgggcgcc
     4381 cgtgtggcga ccggcgcctt gcctgccgcg gccgtagcga tcggcgatgc cgatggcgag
     4441 atcgcgagca aaatcttccc ggcggccgac aagccattca cgcgtgacag cgcgttcttc
     4501 cttgcctcgg tcacgaaacc aatcttcgcc acggccttta tgacgctcgt cgaggagggg
     4561 cggattgacc tgtatcagcc cgtcgaggga ctgctgccgg agttcaccgc cgacaaggac
     4621 gcccgaaaag ccggcgtgac gtggtggcac ctcctgacgc acacatcagg agtgcccgat
     4681 gtctcgcccg aggtaatccg tcgcaccaga ccatccgcga cgcgcatgac tgagatgacg
     4741 cttgccgcgc cgctcgtgtc ggagccaggc actcgctacg agtactgcac cgcgacgttc
     4801 tacctgctgg cgcgggcgat ggagcgcgtc agcgggctca catatgcccg ctatctgcag
     4861 gaacgcgtgc tcgatcccct gggcatggag tcgacattcg atgcgcgcac gtcgcgccgg
     4921 cccatcgtgg ctgtgcaggg cgtgggggtc gaaaaccgac tggttcggtt cctcatcgtg
     4981 cgctacctgg ccggcacggc actgcccggt ggcgggctct tcggcacgct cgacgacctg
     5041 ttgcggttcg gcgcggcgat gatccgcccg gttgacggcc gcgtcccgat caac
//
LOCUS       DATLCO010000019         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_12562, whole genome shotgun sequence.
ACCESSION   DATLCO010000019 DATLCO010000000
VERSION     DATLCO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12562"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /gene="dxs"
                     /locus_tag="VMZ33_00535"
     CDS             complement(<1..1820)
                     /gene="dxs"
                     /locus_tag="VMZ33_00535"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HUR15744.1"
                     /translation="MSLLAKLESPRELKSFDEKQLRQLCAEIRSTIISTVAKTGGHLG
                     SNLGVVELTVALHRLLDSPTDKIVWDTGHQAYAHKLLTGRLDKFDTLRQLGGVGGFPR
                     RTESAHDVFDGGHAGTGLSMAEGLALTRDQHGTDEKVVVIVGDAALMTGMSLEALNDI
                     GVRRTRVLIILNDNEMSISPTVGAMSQYLSRIKLSRTWRGSKRVYDEAINRLPLVGPT
                     ALELSRRLRKSVVNFAQPGQLFEDLGITYVGPIPGHSLRALESTFRRALWDMDGPVIV
                     HVRTQKGRGYQPAVADKVSFHGAALPPMTVVPGGGMGAPSANSVVGGDGDATPSMAQA
                     VAARSKPPNYTTIMAQELIALGKDDPRIVAITAGMPTGTGLSKFQAAFPDRMYDVGIS
                     EQHAMTLATGLALGGMRPLVALYSTFLQRAFDQTVHDVCQNDAPVVIGVDRAGLVGED
                     GTSHQGMFTIPAQRQLPNIVIASPKDEQELRRLMHTAFSQDHPFALHYPRDSGFDLPE
                     VDATAIPVGQAEVVRDGSEILIIGLGPIVYRAAEVADALARDGWSVGLINARFVKPLD
                     RELVTDMARGKKLVVTLEESVVTGGFGSAVLEALAETGLT"
BASE COUNT          323 a          690 c          716 g          375 t
ORIGIN      
        1 tcggtgaggc cggtctccgc cagcgcctcg agcactgccg agccaaagcc gcctgtcacg
       61 acgctctctt cgagcgtgac gacgagcttc ttgccgcgcg ccatgtcggt gaccagctcg
      121 cgatcgaggg gcttgacgaa acgggcgttg atcagcccga ccgaccaacc gtccctggcc
      181 agcgcgtcgg cgacctcggc cgctcgatag acgatgggac ccagaccgat gatcagaatc
      241 tcgctgccgt ctctgacgac ctctgcctgg cccacgggga tggcggtggc gtccacttcg
      301 ggcagatcga agccgctgtc gcgcgggtag tgcagcgcga acgggtggtc ctgcgagaag
      361 gccgtgtgca tcagccgccg cagctcctgt tcgtctttgg gcgaggcgat gacgatgttc
      421 ggcagctggc gctgtgcggg gatggtgaac atgccctggt ggctcgtgcc gtcctcgcca
      481 accagcccgg cgcgatctac tccgataacg actggggcgt cgttctggca cacgtcgtgg
      541 accgtctggt cgaacgcacg ctgcaggaac gtcgagtaga gcgccacgag cggacgcatg
      601 ccgcccagcg cgagtccggt ggcgagcgtc atggcgtgct gctccgatat gccgacgtcg
      661 tacatccggt cggggaacgc ggcctggaac ttggacaggc cggtgccggt gggcatgccc
      721 gcggttatcg ccacgatgcg cggatcgtcc ttgccgagcg cgatcagctc ctgggccatg
      781 atcgtcgtgt agttgggcgg cttcgagcgc gcggccacgg cctgggccat ggagggagtg
      841 gcgtcgccat cgccaccgac gaccgagttg gccgatggcg cacccatgcc gcctcccgga
      901 acgacggtca tcggcggcag cgccgccccg tggaagctca ccttgtcggc cactgcaggc
      961 tggtagccgc ggcctttctg cgtccgcacg tggacgatga ccggcccatc catgtcccag
     1021 agggcacggc ggaaggtcga ctccagggcg cgcaggctgt gcccgggtat gggcccgacg
     1081 tacgtgatcc ccaggtcctc gaacagctgg cccggctggg cgaagttgac gaccgacttg
     1141 cgcagccgcc gactgagctc gagcgcggtc gggcccacaa gtggcaatct gttgatcgct
     1201 tcgtcgtaga cgcgtttgct gccgcgccac gtccgcgaga gcttgatgcg gctcaggtac
     1261 tggctcatgg cgccgaccgt gggcgagatg ctcatctcgt tgtcgttgag gatgatcagc
     1321 acccgtgtcc ggcgcacgcc gatgtcgttc agcgcctcga ggctcatgcc cgtcatcaac
     1381 gcggcgtcgc caacgatcac aacgaccttc tcgtcggtcc cgtgctggtc gcgcgtcagt
     1441 gccaagccct cggccatgct gaggcccgtg cctgcgtgcc cgccgtcgaa aacgtcgtgc
     1501 gcgctctccg tgcggcgcgg gaagccgccg acgccaccca gctggcgcag cgtgtcgaac
     1561 ttatcgagcc gaccggtgag gagcttgtgt gcgtacgcct gatggccggt atcccaaacg
     1621 atcttgtccg tcggcgagtc gagcagtcgg tgcagcgcga cggtcagctc aacaacgccg
     1681 aggtttgacc cgaggtggcc gccggtcttg gcgaccgtgc tgatgatcgt ggaccggatc
     1741 tccgcgcaca gctgccgcag ctgcttctca tcgaaggact tcagctcgcg tgggctctcg
     1801 agtttggcca gaagggacaa gtcagatcac cttgagtttg cgggttcggt cgactcggga
     1861 acgtggacga actcatgccc gctggtgttg tgctgtctga ttctacgcag aggtgatgcg
     1921 ctcggttctc ttgtcggccg aaatcccgac cggtatcagc ggttcggcat caagggggcg
     1981 gctcttcggc ctcgccgagg gataggtcca gcgcccggac cttgccgcca gcgccctcga
     2041 cgagccgctg gaaacgcagc tcggcttcgg tgagcatcct ctcgcaacga cgctggagca
     2101 gcac
//
LOCUS       DATLCO010000020         4808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6714297, whole genome shotgun sequence.
ACCESSION   DATLCO010000020 DATLCO010000000
VERSION     DATLCO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6714297"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VMZ33_00540"
     CDS             complement(<1..339)
                     /locus_tag="VMZ33_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15745.1"
                     /translation="MIVKIEQTGGMMPPWETLRWYPSVALYGDGRLIFQGAQIEIFPG
                     PALPSLWFTHLTEHGIEQVLEWAADAGLQGPDRNLGEQIMDAGANVYTVARPEGTHRT
                     VVHSNASNDPD"
     gene            625..1581
                     /locus_tag="VMZ33_00545"
     CDS             625..1581
                     /locus_tag="VMZ33_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15746.1"
                     /translation="MDELTGKVMTVTGAVDPAALGVTDAHDHLFLASHAMPGQEFQDS
                     EKAIEEVRTAQAGGLATIVDLTPIGLGRRPDLLRQVSDATGLRVVGATGFHRDEHYPD
                     GHWALEATDDVLSVRMLTDIREGMHPTDWVGGDLLDSARAGVIKLGASYQHISRQERR
                     RLEVGGVVSAQTGVSVFVHTEVGTVGDEIVDVLLGAGVGAERIVLAHLDRNPDWEWHA
                     ELLSRGISVVYDTPGRTKYFPDSTLLGLIENVLAAGFGDRVMLGLDLGRRDYYKAYGG
                     GPGLGYLMERFVPRLEKRVGTHAAHQILVANPARVLAVSPIA"
     gene            1578..2864
                     /locus_tag="VMZ33_00550"
     CDS             1578..2864
                     /locus_tag="VMZ33_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR15747.1"
                     /translation="MIRKVGDRYDVIVVGSGSAGSSAAITAGRLGARTLLLDRLAFMG
                     GTSTAVLDTFYAFYTPTDPPRRVVGGLGWEVVERLRDEGVCFERPNTYGAGTGVTYDQ
                     ETLKLTWERLAQDAGVSLLLHTWATGVRVEDGRLRGVRVWNKGGERWFEADVVVDASG
                     DADVAAMAGVPYEDFGTTGKVQSLSTLFRLANVDVAKAAETPKKEMWSLMRSAAASGE
                     YRLPRLEGSWHRTPFDGVVMVHMTRIPNVDATDPVALTAAELEGRRQVREYHRFLRDH
                     VPGFERSVVVGTSPSIGIRESRRVIGDYQLTRDDVLNARRFDDEIALCGAPIEDHGTG
                     GETDWNYVSEGGVYGVPLRCLLPLGVEGLLVAGRCFSATHDAHASARSMATCMAMGQA
                     AGTTAAIGAARGCTPREVTASEVRQRLVDDGAILNV"
     gene            2861..3505
                     /locus_tag="VMZ33_00555"
     CDS             2861..3505
                     /locus_tag="VMZ33_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HUR15748.1"
                     /translation="MSIDPVDLRRGAVAEAIRAQRLIVVLRRVEPQSALLSLVAELVD
                     AGVRAFEITFDSPSAAADLRALRDLLAGRVDGPFVTGAGTLLRGEQVTAAREAGAQFG
                     VSPVLDLEMTRKAVESGFPFVPGAMTPTEMRTAWAAGATFVKLFPASAVGPQFIRELR
                     GPLPEIQVIPSGGVDGDNAIAFLEAGAVAVGLSSAIVRMTAGERRALVESVRRP"
     gene            3502..4281
                     /locus_tag="VMZ33_00560"
     CDS             3502..4281
                     /locus_tag="VMZ33_00560"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR15749.1"
                     /translation="MKRFEGRVCLVTGSTGIAAAAARAMADEGGRVFVVSKTADHAIG
                     LADEFDAGWAAGDLTQEEAVESAVRACIVRFGRIDAVYNVAGISGRRFGDGPLHEMTL
                     DGWRTVIDNNATSIALVCRAAIRQMLTQEPAADGRRGVLLNMSSVLARRPEPGHFATH
                     AYAASKGAIEALTRSAAAYYAKDGIRINAIAPGLVATPMSQRAQTGEGIVEFAKRKQP
                     FAEGGLLKPEDLVGTALYLLSDESRMVTGQVIDVDAGWGLS"
     gene            complement(4278..>4808)
                     /locus_tag="VMZ33_00565"
     CDS             complement(4278..>4808)
                     /locus_tag="VMZ33_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15750.1"
                     /translation="GSIPAEGIQAQELIGAKLAEVAAGAVRLTAAGKLEAADLFAADR
                     SVLGLARAEEQLDEFHALDGRMKQIVTAWQMREIGGEQTFNDHSDPEYDAGVMRELTA
                     LHGDTAEWLAPLGERFSRFGAYSSRLSAALAAAQGGDQRFVASPRVDSYHSVWFELHE
                     DLIRLAGKKRSEATTG"
BASE COUNT          731 a         1514 c         1706 g          857 t
ORIGIN      
        1 atcggggtcg ttgctcgcgt tgctgtggac gacagttcgg tgagtgcctt caggccgcgc
       61 aacggtgtag acgttcgcgc cggcatccat tatctgttcg ccgaggttgc ggtcgggccc
      121 ttgcaggcca gcgtcagcgg cccactcgag gacctgctct attccatgct ccgtcaggtg
      181 cgtgaaccag aggctgggga gtgccggacc gggaaaaatc tcgatctgag cgccttgaaa
      241 gatgagtcgc ccatcgccgt agagcgccac ggacgggtac cacctgagcg tctcccaggg
      301 aggcatcatg ccgccggtct gctcgatctt gacgatcaca gactttgcat cgccgatcgg
      361 cgatggcgtg ccatcgggcg tcggggtggt gtcgggtgag ggaagaggag tgggcgacgg
      421 ggacgtcaac ggtgacgggg agggcgtcgg agatggcgcc cccgtcggcg aagggctgcc
      481 tcctggcgag acgctacagg cagcgaggag gacgactgct gtaacgccga gcgccagccg
      541 ggcagtgcgg ttcatgggcc ttctgacgcg cgagttgccc gatagttccc gcgatgtcgg
      601 ctgcggctgg catcatcgct accgatggat gagttgacgg gcaaggtcat gacggttacc
      661 ggtgctgtcg atccggctgc ccttggcgtg accgacgctc atgaccacct tttcctggcc
      721 tcgcacgcga tgcctggaca ggagtttcag gattccgaaa aggcgattga agaggttcga
      781 acggcgcaag ccggtggcct ggcgacgatc gttgacctga cgcccatcgg gctcgggcgg
      841 cgacccgacc tgttacgcca ggtctctgac gcaacgggcc tgcgcgtggt cggtgccacg
      901 ggctttcatc gagacgagca ctatcccgat ggccactggg ctctggaggc aaccgacgac
      961 gttctgtcgg tgcgcatgct gaccgacatc cgggagggca tgcacccgac cgattgggtt
     1021 ggtggcgacc tgctcgactc cgcccgggcg ggcgtgatca agctcggagc gtcgtaccag
     1081 cacatcagcc gacaggagcg gcgccggctg gaggtcggtg gcgttgtgag cgctcagacg
     1141 ggcgtctcgg ttttcgtgca cacggaggtc ggcaccgtcg gcgacgagat cgtcgatgtg
     1201 ttgcttgggg ccggtgttgg cgcggagcgc atcgtgttgg cgcaccttga ccgcaatccc
     1261 gattgggaat ggcacgcgga gctgctgtcg cgcggcatct ccgtcgtgta cgacacgccc
     1321 gggcgcacca agtatttccc tgactcaacg ctcctgggct tgatcgagaa cgtgctggct
     1381 gccggatttg gcgaccgggt gatgctcggt ctcgaccttg ggcgtcggga ctactacaag
     1441 gcgtacggcg ggggaccggg cctcggctat ctgatggagc ggttcgtgcc gcgcctggaa
     1501 aaacgagttg ggacccacgc agcgcaccag atcctcgttg cgaatccggc tcgggtgttg
     1561 gctgtgagtc cgatcgcgtg attcgcaagg tgggcgatcg gtatgacgtg atcgtggtcg
     1621 gatctggctc cgctggctcg tcggccgcaa tcacggctgg acgtctgggt gcgcggacgc
     1681 tgcttctcga ccgtttggca ttcatgggcg gcacctcgac ggccgtgctc gacaccttct
     1741 acgcgttcta cacgccgacc gatccgccgc ggcgcgtcgt cggcggtctc ggctgggagg
     1801 tcgtggagcg gctgcgcgac gagggtgtct gtttcgagcg gccgaacacg tacggtgcgg
     1861 gcacgggcgt gacgtatgac caggagacgc tcaagctcac ctgggagcgg ctcgcccagg
     1921 acgccggcgt ctcgctgctg ctgcacacgt gggcgacggg cgttcgcgtc gaggacgggc
     1981 gcctccgcgg tgttcgcgtc tggaacaagg gcggtgaacg atggttcgag gctgacgtgg
     2041 tggtcgacgc ttccggtgac gccgatgtgg ctgcgatggc cggcgttccg tacgaggatt
     2101 tcggtacgac cgggaaggtt cagtcgctgt cgacgctgtt ccgtctggcc aatgtggatg
     2161 tggcgaaggc ggccgagacg cccaagaaag agatgtggtc gctgatgcgg tcggcggcag
     2221 cgagtggcga gtatcgattg ccgcggcttg agggttcgtg gcaccgcact ccattcgacg
     2281 gcgtggtgat ggtgcacatg acgcgcatcc ccaacgtgga cgcgacggat ccggttgcgc
     2341 tgaccgccgc cgagctcgag ggccggcggc aggttcgcga gtaccaccgg ttcctgcgtg
     2401 accacgtgcc gggcttcgaa cggtcggtgg tcgtgggcac gtccccgtct atcggtatcc
     2461 gggagtcgcg ccgcgtaatc ggcgattacc agctcacacg cgacgacgtg ctcaatgcgc
     2521 ggcgtttcga cgatgagatc gcgttatgtg gcgcgccgat cgaggaccac ggcacgggcg
     2581 gcgagacaga ctggaactat gtgtcggagg ggggtgtgta cggcgtgccg ctcaggtgcc
     2641 tgttgccact cggcgtggaa gggctgctcg tagccggacg ttgtttctcc gccactcacg
     2701 acgcacacgc gtccgcccgt tcgatggcta cgtgcatggc catgggccag gccgccggca
     2761 cgacggccgc gatcggggca gcgcgcgggt gcacgccgcg cgaggtcaca gcgtccgagg
     2821 ttcgtcagcg gcttgtcgac gacggcgcga tcctgaacgt atgagcattg acccggtcga
     2881 ccttcgccga ggcgcagtcg ctgaggctat ccgcgcccag cggctcatcg ttgttctccg
     2941 gcgcgtcgaa ccgcagagcg ccctgttgtc gctcgtcgcc gagctcgtcg acgccggcgt
     3001 gcgggccttc gagatcacct ttgattcgcc gtctgccgcc gccgacctcc gcgcccttcg
     3061 cgacctgctc gccgggcgcg ttgatggccc gttcgtcact ggagccggca cgctgctgcg
     3121 cggtgagcag gtgacggccg cgcgcgaggc cggcgcgcag ttcggcgtgt cgcccgtgct
     3181 cgatctcgag atgactcgca aagcggtcga gtcgggcttt ccattcgtgc ccggcgccat
     3241 gacgccgacg gagatgcgca cggcgtgggc ggccggagca acgttcgtca agctgttccc
     3301 ggcctctgct gtcgggccgc agttcatccg cgagctgcgc ggaccgctgc cggagatcca
     3361 ggtcatcccg agcggcggcg tcgacggcga caacgcgatc gccttcctgg aggccggcgc
     3421 agtcgccgtg ggcctgtcca gcgcgatcgt ccgcatgacc gcaggggagc gccgcgctct
     3481 cgtcgaatcc gttcgccgcc cgtgaagcgc ttcgagggtc gcgtctgtct cgttacgggc
     3541 tcgacgggga ttgccgcggc cgcggcccgg gcgatggccg atgaaggtgg ccgcgtcttc
     3601 gtcgtgtcaa agacggccga tcacgcgatc ggactcgcag atgagtttga cgccggctgg
     3661 gccgccggag acctgacgca ggaggaggcg gtcgaatcgg ccgtcagggc ttgcatcgtt
     3721 cgcttcggtc gcatcgacgc ggtttacaac gtcgccggca tcagcgggcg gcgtttcggc
     3781 gatgggccac tgcacgagat gacgctcgat ggctggcgca ccgtgattga caacaacgcc
     3841 acgtccatcg cgctggtgtg tcgcgccgcg atccgtcaga tgctcacgca ggaaccggct
     3901 gcagatggtc ggcgtggcgt gctcctgaac atgtcgagcg tcctcgcgcg ccgtccagaa
     3961 ccggggcact tcgccacgca cgcctatgcc gcgagcaagg gcgcgatcga ggcgctgaca
     4021 aggtcggcgg ccgcttacta cgcaaaggac gggattcgca tcaacgcgat cgcgcccgga
     4081 ctggtcgcga ccccgatgag ccagcgcgcc cagactggcg aagggatcgt tgagttcgcc
     4141 aaacgaaagc aacccttcgc cgagggtggg ctgctcaaac ctgaagatct ggtcgggacg
     4201 gcgctgtacc tgctcagcga cgagtcccga atggtcaccg gccaggtcat cgacgtcgac
     4261 gccggctggg gactcagcta gccggttgtt gcctcggagc gcttcttccc ggcgaggcga
     4321 atcaggtcct cgtgtagctc gaaccagacg gagtggtaag agtccactcg cggcgaggcc
     4381 acgaaacgct gatcgccgcc ttgcgcggcc gcgagtgcgg ccgacagccg tgatgagtac
     4441 gcgccaaacc ggctgaaccg ctcgccgaga ggcgcaagcc actcagcggt gtccccgtgg
     4501 agtgccgtca gctcgcgcat tacacccgcg tcgtactccg gatcgctgtg gtcgttgaac
     4561 gtctgctcgc cgccgatctc gcgcatctgc cacgcagtca cgatctgctt catccggccg
     4621 tccagcgcat ggaactcgtc gagctgctcc tccgcgcggg ccagtccaag gaccgatcga
     4681 tccgccgcga acagatcggc agcctccagc tttccagccg cggtcaggcg gacggcccct
     4741 gccgccacct ccgccaactt ggcgccgatc agctcttggg cctggatccc ttcggcggga
     4801 attgatcc
//
LOCUS       DATLCO010000021         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5231953, whole genome shotgun sequence.
ACCESSION   DATLCO010000021 DATLCO010000000
VERSION     DATLCO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5231953"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VMZ33_00570"
     CDS             complement(<1..414)
                     /locus_tag="VMZ33_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR15751.1"
                     /translation="MTDSTRGATALIVDDSAVNRKLLARHLETLGIASDEAIDGHAAL
                     EYLAEHAADVAVVLLDVVMPGLDGYATLEAIKSDEALRRLPVIMISGVDEQASVARCL
                     EMGAADYLPKPFNPVILGARVRASLAEKRMHDLEAE"
     gene            complement(411..773)
                     /locus_tag="VMZ33_00575"
     CDS             complement(411..773)
                     /locus_tag="VMZ33_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HUR15752.1"
                     /translation="MSDEAPLLDHAVIDELRDSVGGDTAFVTELIETYLAEAPGQIDE
                     IVAAASSGDCDAIVRPAHTLKSSSASLGAARLAQIAREIELAGREGRSEGLAQTADSA
                     RSTYEATVEAITAAGLRL"
     gene            complement(770..>2188)
                     /locus_tag="VMZ33_00580"
     CDS             complement(770..>2188)
                     /locus_tag="VMZ33_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR15753.1"
                     /translation="ALLTIINDILDFSKIEAGRVDLSSEPFSLAECIEGALDVITPAA
                     ASKGIELAYEVSEDLPTAVVGDFGRLRQVLLNLLSNAVKFTERGEVVVTVGGTQTGND
                     LRLTVAVRDTGIGIPDDQMGRLFQSFSQADSSIARRYGGTGLGLAISRRLAEAMDGSL
                     TAASTGVPGDGATFHLTVRLPVAPTSSLPAPRSTATVDLRGKRALIVDDNATNRRILS
                     AQLARWEMEVRQTESPHEALDWIRKGQEFEVCLLDLFMPDLDGIALAEQIRAARPTGS
                     PRLVLVSSAAMREHGQTVLDALLTKPVKPSALYDTLVNVLSDAGPSQRDESRPAQSSA
                     DADLGARVPHRILLAEDNAVNQKLALRLLSNMGYAADVAGDGLQAIAALETTDYDLVL
                     MDVQMPELDGLEATRRIRARWPDRAVHIVAMTANAMAGDRDACLAAGMNDYVSKPIRT
                     AELARALRQAPSNVSVGQGEAT"
BASE COUNT          355 a          709 c          743 g          381 t
ORIGIN      
        1 ctcagcctcg agatcgtgca ttcgcttttc ggcgagcgag gcacgaacac gtgcgccgag
       61 gatcaccggg ttgaacggct tgggcaggta gtcggccgcg cccatctcca ggcagcgtgc
      121 gacggaagcc tgctcatcga cgcccgaaat catgatcacc ggcaggcgac gcagcgcctc
      181 gtcgctcttg attgcctcca aggtggcgta gccgtcgagc ccgggcatca cgacgtcgag
      241 cagcacgacg gccacgtcag ctgcgtgctc ggcgaggtac tcgagtgcgg catgaccgtc
      301 gatggcctcg tcgctggcaa tgcccagggt ctcgagatgg cgcgccagca gcttccggtt
      361 gaccgcactg tcgtcgacga tgagagcagt cgcgcctctc gtggagtcgg tcacaggcgc
      421 aaccctgccg cggtgatggc ctcgaccgtg gcctcgtacg tcgaccgagc cgaatccgct
      481 gtctgggcaa gaccctctga ccggccttcc cggcccgcga gctcgatctc gcgtgcgatc
      541 tgcgcgaggc gagccgcgcc cagggacgcg ctgctcgact tgagcgtgtg tgccggccgg
      601 acaatggcgt cgcagtcgcc actcgacgcc gcggccacaa tctcatcgat ctgccccggg
      661 gcctcggcga gatacgtctc gatgagctcg gtcacgaacg cggtgtcgcc gccaaccgag
      721 tcacgcagct cgtcgatcac ggcatggtca agcagcggtg cctcatcgct cacgttgcct
      781 ctccctgccc gacactgacg ttcgacggtg cctgccgcag agcgcgggct agctcggcgg
      841 tccgaattgg cttgctgacg tagtcgttca ttccggcggc taggcatgcg tcgcgatcgc
      901 cggccatcgc gttggctgtc atggccacga tgtggaccgc tcgatccggc catcgcgcgc
      961 ggatgcgccg cgtcgcttca aggccgtcga gctcgggcat ctggacatcc atcaggacga
     1021 ggtcgtaatc ggtcgtttcg agtgcagcga tggcctgcag gccgtcgccc gcgacgtcag
     1081 cggcgtagcc catgtttgac agcaggcgca gcgcgagctt ctggttgacc gcattgtctt
     1141 cagccagaag gatgcgatgt ggaacccgtg cgccgaggtc ggcgtcggcc gatgactgcg
     1201 ccggccggga ttcatcgcgt tggctcggcc ccgcatctga cagcacgttg accaacgtgt
     1261 cgtagagcgc ggacggcttg actggcttgg tgagcagagc gtcgagcacc gtttgcccgt
     1321 gctcgcgcat ggctgccgac gacacaagaa ccagccgcgg ggagccagtt ggtcgagcgg
     1381 cgcggatctg ctcggcgaga gcgataccgt cgaggtcggg catgaacaag tcgagcaggc
     1441 acacctcgaa ttcctggcct ttccgaatcc aatcgagggc ttcgtggggg gattcggtct
     1501 gacgaacctc catctcccag cgtgcgagct gtgcgctgag gatccgcctg ttggtggcgt
     1561 tgtcatcaac gatcagcgcc cgcttgccgc gcaggtcaac cgtggctgtg gagcgtggcg
     1621 ccggcaagga cgatgttggt gcgacgggca agcggacggt caggtgaaat gtcgcgccgt
     1681 caccgggaac gcccgtgctc gcggcggtaa gcgacccatc catggcctcg gcaaggcgcc
     1741 ggctgatcgc caggccaagg ccggtgccgc cgtagcgccg ggcgatcgac gagtcggcct
     1801 ggctgaacga ctggaacagg cggcccatct ggtcgtccgg tatgccgatg cccgtgtcgc
     1861 gaacggcgac tgtcagccga aggtcgttgc cggtttgagt gccgccgacg gtcacaacaa
     1921 cctcgccgcg ctctgtgaac ttgaccgcgt tcgagagcag gttgagcagc acttgtcgca
     1981 gccgaccgaa gtctccgacc acggcggttg gcaagtcctc gctgacctcg tacgcgagct
     2041 cgatgccctt cgacgccgca gccggggtga tcacgtcgag ggcgccctcg atgcactcgg
     2101 ccaggctgaa cggctcggat gagaggtcca cacggccggc ctcgatcttc gagaagtcga
     2161 ggatgtcgtt gatgatggtc agcaaggc
//
LOCUS       DATLCO010000022         5027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10441483, whole genome shotgun sequence.
ACCESSION   DATLCO010000022 DATLCO010000000
VERSION     DATLCO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10441483"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VMZ33_00585"
     CDS             <1..890
                     /locus_tag="VMZ33_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HUR15754.1"
                     /translation="IVLILIATFIALRFAHITVDAALNRLFERELTEGTVQDLSATEV
                     QRRRDTLEGLVYRAVRVIILVIAFLMTLQVLSLDIGPAIAGLGIVGLALSLGAQHLVR
                     DYVAGAFVLIENQYSKGDVVTIAGVSGTVEDVSLRRTTLRDHDGTVHYVPHGLIQTAS
                     NHTRNWAAIEIDLPMTYEQDLAALRDAVGAAGKRLAADQKWRAAIFEAPHVMSVERLA
                     ETGLLVRVSASVAALHRYVLPGVIRGLVLEECTSRGIVIGWREAPAPEPSKAAKPTPT
                     DPSAEAMAPLVMPPPGADD"
     gene            900..2894
                     /gene="acs"
                     /locus_tag="VMZ33_00590"
     CDS             900..2894
                     /gene="acs"
                     /locus_tag="VMZ33_00590"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HUR15755.1"
                     /translation="MTDTSLPGSLSEPELENLLAETRRFPPAPEFSAQANATADLYEE
                     AERDYVAFWERQARERLTWFEPFTKTLEWEPPFSKWFLGGKLNVAYNCVDRHVENGLG
                     DKVAYHWIGEPGDTRTITYADLQREVSKSANALKELGVKSGDVVAIYMPMIPELPIAM
                     LACARIGAPHTVVFGGFSAEALAGRIEDAQAKLIITADGGWRRGKTANLKQAADDAAQ
                     MSPTIEHVLVVRRVGEAGESGEVTMQEGRDVWWHDIVDRQSADCPPVPLDSEHMLYLL
                     YTSGTTAKPKGIMHTTAGYLLGAAYTHWAVFDVKPDDVYWCAADVGWVTGHSYIVYGP
                     LANATTCVMYEGTPDTPAWDRWWQIIEDYKVSILYCAPTAIRAFMKQGEEWPERHDLS
                     SLRLLGSVGEPINPEAWLWYQHNIGSGSTPIVDTWWQTETGMIMITPLPGVTTTKPGS
                     ATFPFPGIGVDVLDARGNSVPLGGGGYLVLTRPWPAMLRGIYGDPERFKATYWSRFPG
                     KYFAGDGAKRDEEGYLWLLGRVDDVMNVSGHRLSTIEVESALVHHPAVAEAAVVGRAD
                     PVTGQAIFAFVILRVGIDPTDELAAEVRDHVAKVISPIAKPKYLLFTPDLPKTRSGKI
                     MRRLLRDIGEGRTLGDTTTLADSSVVELIREKSGAATEEE"
     gene            2956..3384
                     /locus_tag="VMZ33_00595"
     CDS             2956..3384
                     /locus_tag="VMZ33_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HUR15756.1"
                     /translation="MSNQEVARWFAIATGVVLVLIGLLGFINNPIVGDPSPPNSSAPL
                     FVTGTMHNIVHLVTGAVALYIGFMLKGDAQANALIGFGALYAVVLVATLLSPNLFGLL
                     GDNRYNVNTMDHVLHLALAVLSIGIGYWARGRDTVAPRRT"
     gene            3473..4525
                     /gene="moaA"
                     /locus_tag="VMZ33_00600"
     CDS             3473..4525
                     /gene="moaA"
                     /locus_tag="VMZ33_00600"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HUR15757.1"
                     /translation="MNPLATSPVVDRLGRAIHDLRISVTDRCNFRCTYCMPREIYGRD
                     FAFLPRAELLTFEEITRLARVFAGLGVRKLRLTGGEPLLRRDLPVLVGLLSKIDDVDD
                     LTLTTNGSLLARQAQALADAGLTRVSVSLDSLDEATFARMNDVGFPVTAVLAGIAAAA
                     AAGLRPIKVNMVVRRGVNETSVLPMARFFRERGHILRFIEYMDVGHTNGWRMDDVVPA
                     SEILAMIDAELPLVAVPPLYGGEVADRWSYRDGSGEVGVIASVTQPFCADCTRARLTA
                     DGQLFTCLFSNIGHDLREPLRAGASDEHLVDAIIAVWRGRDERYSELRSLQTLPAGAA
                     IVVADPARVEMSRIGG"
     gene            4606..>5027
                     /locus_tag="VMZ33_00605"
     CDS             4606..>5027
                     /locus_tag="VMZ33_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HUR15758.1"
                     /translation="MTMDQSGAGQAAPPVSQPPAAPPPPTGWQSTPTPPGAPPPAGGQ
                     PGGMPSWTNNITARGTIAGPGGVAVADTPDRVIAAVIDFIILGVIGFIVGAIIHPILG
                     EKFGAGTLFQFTVPSLVSSIAVALIMLAVAGAYFIGMWT"
BASE COUNT          821 a         1695 c         1650 g          861 t
ORIGIN      
        1 agatcgtcct gatcctgatc gcgaccttca ttgccctgcg cttcgcgcac atcacggtcg
       61 acgccgcgct caaccgcctg ttcgaacgcg agctgaccga gggcacggtt caggacctct
      121 ctgcgacgga ggtccagcgc cggcgggaca cgctcgaggg cctcgtctat cgcgccgtgc
      181 gcgtgatcat cctcgtcatc gcattcctga tgacgctgca ggtgctgagc ctggacatcg
      241 ggccggcgat cgcgggcctg ggcatcgtcg ggctcgccct cagcctcggc gcgcagcatc
      301 tcgtccgcga ctacgtggcc ggcgcgttcg tgctcatcga gaaccagtac tcgaagggcg
      361 acgtcgtcac gattgccggc gtaagtggca cggtcgagga tgtctcgctc cgccgcacga
      421 ccctgcgcga ccacgatggg acggtccact acgtcccgca cggcctgatc cagacggcca
      481 gcaaccacac gcgcaactgg gcggcgatcg agatcgacct gccgatgacg tacgagcagg
      541 atctcgcggc cctgcgggat gccgtggggg cggccggcaa gcggctcgcg gcggaccaga
      601 aatggcgggc cgcgatcttc gaggcgccgc acgtcatgag cgttgagcgg ctcgctgaga
      661 cgggactgct cgtgcgcgtc tcagcttccg tcgcggcgct ccaccgctat gtgctgccgg
      721 gcgtgatccg tggactagtc ctcgaggagt gcacgagccg cggcatcgtg atcggctggc
      781 gcgaggcgcc cgcgcccgag ccgtcaaagg ccgccaagcc aaccccgaca gacccgtcgg
      841 cggaggcgat ggcaccactc gtcatgccac cgccgggtgc tgacgattag catcggccca
      901 tgaccgacac gtcactgccg ggctctctct ccgagcctga gctcgagaac ctcctcgccg
      961 aaacacgacg ctttcccccg gcacccgagt tctcggccca ggccaacgcc acggccgacc
     1021 tgtacgagga agccgagcgc gactatgtgg cgttctggga acgacaagcg cgcgagcgcc
     1081 tgacctggtt cgagcccttc acgaagacgc tcgagtggga accgcctttc tcgaaatggt
     1141 tcctcggcgg caagctcaac gtcgcgtaca actgtgtcga tcgccacgtc gagaacgggc
     1201 tcggcgacaa ggtcgcgtac cactggatcg gcgagccggg cgacacgcgg accatcacct
     1261 acgccgatct ccagcgcgag gtgtcgaaaa gcgccaacgc gctgaaggag ctcggcgtga
     1321 agtccggcga cgttgtcgcg atctacatgc cgatgatccc cgagctgccc atcgcgatgc
     1381 tcgcctgcgc acgaatcggc gcgccgcaca cagtcgtctt cggtggcttt tcggccgagg
     1441 ccctggcggg gcgcatcgag gacgcccagg ccaagctgat catcaccgcc gacggtggtt
     1501 ggcgtcgggg caagacggcg aacctcaagc aggccgccga cgacgccgcg cagatgtcgc
     1561 caacgatcga gcacgtgctc gtcgtgagac gggtcgggga ggcgggcgag agtggcgagg
     1621 tcacgatgca ggagggacgc gacgtctggt ggcacgacat cgtcgaccgc cagagcgcag
     1681 attgcccgcc ggtgccgttg gattcggagc acatgctcta cctgctgtac acgtcgggta
     1741 cgacggcaaa gcccaagggc atcatgcaca ccacggcggg ctatctcctg ggcgcggcgt
     1801 acacccactg ggccgtgttc gacgtcaagc cggacgacgt ctactggtgc gcggctgacg
     1861 tgggttgggt taccggccac tcgtacatcg tctacgggcc gctcgccaac gcgaccacct
     1921 gcgtcatgta cgagggcacg cccgacacgc cagcttggga tcgctggtgg cagatcatcg
     1981 aggactacaa ggtcagcatc ctgtactgcg ccccgacggc gatccgggct ttcatgaagc
     2041 agggcgagga gtggcctgag cgccacgacc tctcgtcgct gcgactgctg gggtccgtgg
     2101 gtgagcccat caaccccgag gcgtggctct ggtaccagca caacatcggg agcggcagca
     2161 cgcccatcgt cgacacgtgg tggcagaccg agacgggcat gatcatgatc acgccactcc
     2221 ccggcgtcac caccaccaaa ccgggcagtg cgacattccc gttcccgggg atcggggtag
     2281 acgtcctcga cgctcgcggc aactccgtgc cgttgggcgg cggcgggtac ctcgtcctga
     2341 cgcggccatg gcctgccatg ttgcgcggca tctacggcga tccggagcgc ttcaaagcga
     2401 cctactggag ccgattcccg ggcaagtact tcgccgggga cggcgcgaag cgggacgagg
     2461 aggggtacct ctggctactc ggccgcgtgg atgacgtcat gaacgtatcg ggccatcgcc
     2521 tgtccacgat tgaggttgag tcggcgctgg tccaccatcc cgcggtggct gaggcggcgg
     2581 tcgtcggtcg ggccgatccc gtgaccgggc aagcgatctt tgccttcgtg atcctgcggg
     2641 ttgggataga tccgaccgat gagctggctg ccgaagtgcg cgatcacgtc gccaaggtca
     2701 tcagcccgat cgccaagccc aaatacctgc tgttcacgcc cgacctgccc aagacgcgct
     2761 caggcaagat catgcgtcgg ctcctgcgcg acatcggtga ggggcgcact ctgggcgaca
     2821 cgaccacctt ggccgattcc agcgtggtgg agctgatacg cgaaaaaagt ggagccgcca
     2881 ctgaggagga gtgatccgta gccgttcctg ccgcgaagta cagaccacag gcttaattca
     2941 agggaggttt gagagttgtc caaccaggaa gtagctcgct ggttcgcgat cgctacgggc
     3001 gtggtgctcg tactcattgg tttgctcggc ttcatcaata atccgatcgt cggcgatccc
     3061 tcgccgccca actcctctgc gccactgttc gtgaccggga ccatgcacaa catcgtgcac
     3121 ctcgtcaccg gagctgtcgc gctgtacatc ggcttcatgc tcaagggtga tgcccaggcc
     3181 aacgccttga tcggcttcgg tgccctgtac gccgtcgtcc tcgtggccac gctgctcagc
     3241 ccgaaccttt tcggtctgct cggtgacaac cgctacaacg tcaacaccat ggaccacgtt
     3301 ctgcacctgg ccctcgcggt gctcagcatc ggcatcggct actgggctcg cggccgcgac
     3361 acggtcgcgc cgcgccgcac ctagcttcct tcgctcctcc tcctctggcc gcccgcccct
     3421 gccggggcgg gcggccttct cgattcacgg cgcccttaac atcaatccgg caatgaaccc
     3481 gttagcgacc tcgccggtgg tggatcggct gggtcgggca atccacgacc tgcgcatctc
     3541 cgtcaccgac cgctgcaact tccgctgcac gtactgcatg ccgcgcgaga tctacgggcg
     3601 cgattttgcg ttcctgccac gggccgagtt gctcacgttc gaggagatca cgcgcctggc
     3661 ccgggtgttc gcaggcctcg gggtgcgcaa gctgcggctg accggcggtg agccactgct
     3721 gcggcgtgac ctgccggtgc tggtgggcct gctctcgaag atcgacgatg tcgatgactt
     3781 gacgctgacg acgaacggct cactgctcgc ccgtcaggcc caggcgttgg cggacgctgg
     3841 gctgacacgg gtgtcggtca gcctcgactc gctcgacgag gcgacgttcg cgcgaatgaa
     3901 cgacgtcggg ttcccggtga ctgcggtgct tgcggggatc gcggcagccg cggcggcggg
     3961 cctgcggccg atcaaggtca acatggtcgt gcgccgcggc gtcaacgaga ccagcgtgct
     4021 gcccatggcg cgcttcttcc gcgaacgcgg tcacatcctg cgcttcatcg agtacatgga
     4081 cgtcggccat accaatggct ggcggatgga cgacgtcgtt ccggcgtcgg agatcctggc
     4141 catgatcgac gccgagctgc ccctcgtggc cgtaccgccg ctctatggcg gcgaggtggc
     4201 ggaccgctgg tcgtaccggg acggcagcgg cgaggtcggg gtcattgcct ccgtcacgca
     4261 acccttctgc gcagactgca cccgcgctcg tctcacggcc gacggccaac tcttcacgtg
     4321 cctcttctcg aatatcggcc acgacctgcg cgaaccgctg cgtgccggcg ccagcgacga
     4381 gcatctggtc gacgccatca tcgccgtgtg gcgcggccgg gacgagcgct attccgagct
     4441 gcggtccctg cagacgttgc ccgcaggcgc cgcgatcgtt gtcgctgacc ccgcgcgcgt
     4501 ggagatgtcc cgaatcggcg gttgagtcgc ggcaaatttt cctttccggc gggtttcgtg
     4561 ttgggtatgc tgcgagcgtc acactgggca tagaggagga gagagatgac catggaccaa
     4621 tccggcgccg gccaagcggc cccgccagtt tcacagcccc ccgcggcacc gccgccgccg
     4681 acgggctggc agtcgacacc gacaccgcca ggcgcgccgc cgcccgctgg tggtcagccg
     4741 ggcggcatgc cgtcgtggac gaacaacatc acggctcgcg gcacaatcgc aggccccggt
     4801 ggagtggccg tagctgacac accggatcgc gtcattgccg ccgtgatcga cttcatcatc
     4861 ctgggggtga tcgggttcat cgtgggggcg atcatccatc ccatcctcgg cgagaagttc
     4921 ggggccggaa cgcttttcca gttcacggtc ccttcattgg tgagcagcat cgccgtcgcg
     4981 ctgatcatgt tggccgtcgc cggcgcgtac ttcatcggta tgtggac
//
LOCUS       DATLCO010000023         8271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9698049, whole genome shotgun sequence.
ACCESSION   DATLCO010000023 DATLCO010000000
VERSION     DATLCO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9698049"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VMZ33_00610"
     CDS             complement(<1..953)
                     /locus_tag="VMZ33_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15759.1"
                     /translation="MNRALAAAAIEAAQRYGTPLYLYDLARLRADIAAITAAFPDPWI
                     RLYSMKANGLPALVAEVGRTGFGISAVSGGELSLARKAGVPPHRLALEGIGKTDADLR
                     VAARINPLWVSLETAQDAAAMARAMSRSERRQDVLVRVNPQVAPETHHGLAVGAPISK
                     FGVLPEELSGVVEAGGGPDGPLRWRGIHLHVGSQLGAVDAWRSAFRVGVRLLELQRAE
                     LPDFDTLDAGGGFPVDYGAAQDGSVPAVAIFAAAAHAEIDALAASAQPRRLAIEPGRA
                     VVAASGWVIGRVLHVRRRDPDIIVIDAGMTELIRPALYGAE"
     gene            complement(950..1351)
                     /locus_tag="VMZ33_00615"
     CDS             complement(950..1351)
                     /locus_tag="VMZ33_00615"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HUR15760.1"
                     /translation="MLDIVIRVVVNAIAFVAAIKLVPRADFGPDLWKLVLVAAIFGVV
                     NAYLRPIVKTLSIPLSLITLGAVGFVINAAMVLLTGAISERLDLGLRLAGWPVRGEID
                     LDVIITAVLVSLVVSVVATLLAVVRMVTPRV"
     gene            1475..3265
                     /locus_tag="VMZ33_00620"
     CDS             1475..3265
                     /locus_tag="VMZ33_00620"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUR15761.1"
                     /translation="MTVDFVNLGADNRPEPRTAFDHALHKLLDDWFAAEPTWATAVGF
                     HAFDDRWPDPSDAGRTGRLAMLRHHRARLQALSDNELSPSERLDRGIVLDAIDGAEFA
                     DAELRDGACDPLFYVYQAGAGLFNILARDYAPWRHRGSAFLGRVERLTDFLSAASEAL
                     TGLPNRPVSELHLKTALEQLDGISELVDQGAAEAKEHSAEEPEIARALAEQAPRAKQA
                     VEEFARRLREEIAPRATGEGRLGGELFGQKLRHTLSSELTYTDLQQQAREDYAKVRTE
                     ILRLAREAWGEWLPGQAMPTDDQEMIRAVLDAIARVHRQPEELIDWCREEVHRIEDFC
                     RQNNVIGLPDDPLQITWTPVFMRAYGRAFLESPGVLDKGLSSYFWITPPDEALGPEAV
                     ESYLREDNDRMLKLLCIHEGVPGHYLQLSWSNRTPSLARAVFQNGMFAEGWAVYVTQV
                     LMDLGYGDHEPALMLTHWKFYLRAVINTLIDIGIHTGDMTEDEAIDLMVNGGFQEEDE
                     ARAKWLRARLTSTQLCTYYTGSLEMWNMEIEARRRAATGAGESPDTLPQQRIVGGLGD
                     TPGFDYRSHLESVISHGSPPIKWVSRILFD"
     gene            3280..4164
                     /locus_tag="VMZ33_00625"
     CDS             3280..4164
                     /locus_tag="VMZ33_00625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HUR15762.1"
                     /translation="MDFGLILPSYRVGATTESIDAASETAARLGWHSVFTTDHLLVEP
                     SEQSRDYFNIYDAVVTLAHIAAKQPTLKVGASVVVVPMRNALVLAKELATVDALSGGR
                     LIVGVGVGWNQTEFGYVGEADRFSVRGAYLEEAVNLWRHLWSGNEAPFEGRFHSFGEV
                     RFGPLPAQRENVPIWFGGRDEKALRRCGRLGDGYHSSATTPDRYAPRVPLIREAARDA
                     GRPEPVFSARFRVAFEPRTTGPFYTLTGDPDEMIADVRAFAGAGVTHVAVDFAETDAE
                     QARDLIERFDREVVAAFR"
     gene            4161..5015
                     /locus_tag="VMZ33_00630"
     CDS             4161..5015
                     /locus_tag="VMZ33_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR15763.1"
                     /translation="MTAPRRPETARPRQDSPESHEHGLPTDEELDAIELQHATRMVQS
                     GAEVTADEELGVWWIRFERRTPQLAFAMRLRWPEAEAETRLARLTAVMQGRGEWPSLS
                     ISEGLTQPTTIGDTLADAGWRIVAGERLMFTRHPPTNPHLDPTLRIVAVTPATAKECV
                     ELEITNFGLPLSEVEPRTEWLAEAVRNGSERAYIVRSMHKPVASARLIPGAGGAACLS
                     AINVVAAQRGRGYGRLVTSVATRAGLATGAKLVWLSVDESNAPAVKLYESLGFEPTFA
                     WSRWIASA"
     gene            complement(5052..7163)
                     /locus_tag="VMZ33_00635"
     CDS             complement(5052..7163)
                     /locus_tag="VMZ33_00635"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HUR15764.1"
                     /translation="MTDRISLALVFHNHQPVGNFGWVFEENYERSYEPMIGALERHPR
                     IRVGLHYTGPLLEWMVSERPESIERIRALVARGQVEILGGGLYEPILVSLPQRDRHGQ
                     LMRMRSELERVFGAAPVGAWLAERVWEPSVPYDLASAGYEYTIVDDNHLRGAAVHDED
                     MWGTYTTDDQGRRLTVFTTEKGLRYHIPWKPVDDLIGYLRQNATADGKRLGMMGDDGE
                     KFGGWPGTHDLCWGEEQWVDRCFTALETNSSWVSTVRPSDWMDGQPSIGRIYIPTSSY
                     VEMTEWALPPERALEFHHVVEKAEKAEKAKPWAPFVRGGLWRNFQARYREINDLHKQM
                     LGVSSAVQAMTDGPIKDLATDHLHRAQSNDCYWHGLFGGIYIVHMRMATYHHLIAADD
                     LARLGEASSGIADFDLDGADEVLLGTPGQSVLVDIAEGAGIGSWDLRATRVALAAVLR
                     RRPEAYHEKLRRAEAAGTTKSADPEELSNPHEDMRQKESNLSRFLTYDNYERRSGLVR
                     VLARDGSEVGDFVHGEWTLAEVGADAARLARATDDLIVTKSVRLGGNRLDPRLTLDVE
                     VASRSGSEFHGVLEVEWNINLSGGGRNLSAYYRWADGEMRHDEAGSVEPGTALRFGNE
                     YDGVDIAASAEPAAAASWYPIETVSNSEAGFERMYQGSCLLFRWPLKLEAGGSATFSL
                     TMAVSQSRDRSAEEIAELDAD"
     gene            complement(7195..>8271)
                     /gene="glgP"
                     /locus_tag="VMZ33_00640"
     CDS             complement(7195..>8271)
                     /gene="glgP"
                     /locus_tag="VMZ33_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HUR15765.1"
                     /translation="DTNGANAVSQLHAETANATWLGIVEQPILGITNGVHPPSWVGDP
                     FRRLYEQLDGVLTNLDKEESPFWERIGKLADNQIWEAHKRQKLEFAFFCRRRLQNQFA
                     RHGEAPALLESLADALDPDVLTIGFARRFATYKRAALIFSDEERLGRILNNVDRPVQI
                     IFAGKAHPADRPGQKVIQDIFGKSRVAKFTNRVFVLEDYDIRVGRYLVQGVDVWLNNP
                     RRPLEASGTSGMKAAMNGVINFSVRDGWWDEGYDGHNGWSIGSREQNPDEGAQDWADA
                     QDLYRLLEQEIVPTFYERNANGIPARWIERMKASMASTIWQFSTTRMLQEYVERLYGP
                     AAERGRRRRSGKRDEPVKTGQAAA"
BASE COUNT         1376 a         2790 c         2702 g         1403 t
ORIGIN      
        1 tgctcggcgc catagagggc cggacgtatg agctcggtca tcccggcgtc gatgacgatg
       61 atgtcgggat cgcgacgtcg gacgtggagc actcggccga tcacccaccc cgaagcagcc
      121 accacggctc tgcccggttc gattgccagc ctgcgcggct gggcgctagc tgccagggcg
      181 tcaatctccg catgtgccgc ggccgcaaat atggccacgg ccggaacgct cccgtcctgc
      241 gctgcgccgt agtcgaccgg aaaaccgccg ccggcatcga gcgtgtcgaa gtcgggcagc
      301 tcagctcgtt ggagctccaa cagtcgaacg ccgacgcgaa aggctgagcg ccaggcatcg
      361 acggccccca gctgcgatcc aacgtggagg tggatgccgc gccagcggag tggcccatcc
      421 ggcccgccgc cagcctcgac gacgcccgac agctcttccg gcagcacccc gaacttcgag
      481 atcggcgcac caaccgccaa cccgtgatgt gtctcgggcg cgacctgcgg gttgacgcgc
      541 accagcacgt cctgtcgtcg ctcggagcga ctcatggccc gcgccatcgc ggcggcatcc
      601 tgcgcggttt cgaggctgac ccacaacgga ttgattcgcg ccgcaactct cagatcggcg
      661 tcggtcttgc cgatcccctc cagcgccagc cgatgtggtg gcacacccgc cttgcgcgcc
      721 agcgatagct cgccgcccga gacggcgctg atcccgaagc ccgttcgccc gacctcggcg
      781 acgagcgccg gcaggccgtt ggccttcatc gagtagaggc gaatccacgg gtccggaaac
      841 gcagccgtga tcgccgcgat gtcggcgcgg agccgcgcca ggtcgtacag atagagagga
      901 gtgccgtatc gctgggccgc ctcgatcgca gccgcggcga gtgccctgtt cagactcgcg
      961 gcgtcaccat tcggaccacg gcgagcaacg ttgcgacgac gctcaccacg agtgagacga
     1021 gcacagcagt gatgatcacg tcgagatcga tttcaccgcg aacgggccag ccggcgagac
     1081 ggaggcccaa gtcgagacgc tcgctgatag cgcccgtcag caggaccatg gccgcgttga
     1141 tgacgaagcc cacggcaccg agcgtgatca acgacagcgg gatgctgagc gtcttcacta
     1201 tcggccgcag ataggcattt acgacgccga agatcgcggc gacgaggacg agcttccaga
     1261 ggtcaggccc gaaatcggcg cgaggaacaa gtttgatcgc cgcgacaaac gcgatcgcgt
     1321 tgaccacgac acgaatgacg atatccagca ctgctctacc tccgcaggcg ccgccctcgc
     1381 ctgcctctgg cgacccgccg cgttgcggct gatcagcctg ggtttgcagg ctcgttcgca
     1441 tgctacatgc gccaaccgtc gataggatgc cgccgtgacc gtcgatttcg tcaacctcgg
     1501 agccgacaat cggcccgagc cgcgcactgc ctttgaccac gccctgcaca agctcctcga
     1561 cgattggttc gccgccgaac cgacgtgggc gacggccgtt ggcttccacg ccttcgacga
     1621 tcgctggccg gatccgagcg acgctggccg gactggtcgc ctggcgatgt tgcgccacca
     1681 tcgcgcacga ctacaggcac tgtccgacaa cgagctgtca ccgtccgaac gactcgaccg
     1741 cggcatcgtc ctggatgcca tcgatggcgc ggagttcgcg gacgccgagc tgcgcgacgg
     1801 tgcttgtgac ccgctcttct acgtttatca ggccggtgcc ggcctcttca acatcctcgc
     1861 ccgcgactac gcgccgtggc gccaccgcgg gtcggcattt ctcggccggg tggagcgcct
     1921 gaccgacttc ctgagcgccg caagcgaggc gttgaccggg ctgcccaatc gtccggtttc
     1981 agagctgcac ctcaaaacgg ctctggaaca gctcgatggc atctccgagc tcgtcgatca
     2041 aggcgcggcg gaggccaaag agcacagcgc ggaagaaccg gagatcgcgc gcgccctcgc
     2101 ggagcaggcg ccgcgtgcga agcaggccgt tgaggagttt gcgcggcgtc tgcgcgaaga
     2161 aatcgcgccg cgtgccacgg gcgagggacg gttgggcgga gagctcttcg gccagaagct
     2221 ccgccacacg ctctcgagcg agctgacgta caccgacctc cagcagcagg cgcgcgagga
     2281 ctacgcgaag gtgcgcacgg aaatcttgcg gctggcccgt gaagcctggg gcgagtggct
     2341 gccgggtcag gccatgccga ccgacgatca ggagatgatc cgagctgtcc tagacgccat
     2401 cgcgcgggta catcggcaac cagaggagct gatcgactgg tgccgcgaag aggttcatcg
     2461 gatcgaggat ttctgtcgcc agaacaacgt catcgggctg cccgatgacc cgctgcagat
     2521 cacctggacg ccggtcttca tgcgcgccta tggccgtgcc tttctcgagt cgcccggcgt
     2581 gctcgacaag ggcttgtcga gctacttctg gatcacgcca ccggacgagg cactcgggcc
     2641 ggaggccgtc gagtcgtacc tgcgcgaaga caacgaccgg atgctgaagc tgctctgcat
     2701 ccatgagggc gtacccggcc actacctgca gctgtcgtgg tccaaccgga cgccgagcct
     2761 cgcacgtgcc gtgttccaga acgggatgtt cgctgagggt tgggccgtct acgtgaccca
     2821 ggtgctgatg gatctcggct acggcgacca cgagccggcg ctgatgctca cccactggaa
     2881 gttctacctg cgcgccgtca tcaacacgct gatcgacatc gggatccaca ccggtgacat
     2941 gaccgaggac gaggcaatcg acctcatggt caacggcggc ttccaggagg aggacgaggc
     3001 ccgggcaaag tggttgcgag cgcggctcac gtcgactcag ctgtgtacgt attacacggg
     3061 ctcgctcgag atgtggaaca tggagatcga agcacgtcga cgcgccgcaa ccggcgccgg
     3121 cgagagcccg gacaccctcc cacaacagcg catagtcggc gggctgggtg acacgccggg
     3181 cttcgactat cgctcacacc tggagtcggt catctcgcac gggtcgccgc cgatcaaatg
     3241 ggtcagtcgg atccttttcg actagggacg gcagccaaga tggatttcgg gctcatcctg
     3301 ccgagctatc gggtcggcgc caccaccgag tcgatcgacg ccgcatcgga gacggctgca
     3361 cgcctgggct ggcactcggt attcaccacg gaccacctgc tggtcgagcc gtccgagcaa
     3421 tcgcgggatt acttcaacat ctacgacgct gtcgtcacac tggcgcacat cgccgccaag
     3481 cagccgacgc tgaaggtcgg cgcgagcgta gtggtcgtcc cgatgcgcaa cgcgctggtg
     3541 ctggccaagg agctggccac ggtcgacgcc ctgtcaggtg gccggctgat cgtaggtgtc
     3601 ggcgtcggct ggaatcagac tgagttcggt tacgtgggcg aggccgaccg gttcagcgtt
     3661 cgtggcgcgt accttgaaga ggccgtcaac ctgtggcgtc acctctggtc ggggaacgag
     3721 gccccgttcg agggccgctt ccactcgttc ggcgaggtcc gcttcgggcc actccctgcc
     3781 caacgcgaaa acgtcccgat ctggttcggc ggccgtgacg aaaaggcgtt gcgacgctgt
     3841 ggccggcttg gtgacggcta ccactcatcg gcgactactc cggatcgcta cgccccacga
     3901 gtgccgctca tccgcgaggc tgcccgtgac gccggtcgtc ctgagcccgt gttctcggcg
     3961 cgtttccgcg tggctttcga gccgcgcacg acggggccct tctacacgct cacgggcgat
     4021 ccggacgaga tgattgcgga cgttcgagcc ttcgccggcg ctggcgtgac gcacgtcgcg
     4081 gtcgacttcg ccgagacgga cgccgagcag gcgcgcgacc tgatcgaacg cttcgatcgc
     4141 gaggtagtcg cggcgtttcg atgactgcac cgcgccgacc tgagacggca cggccgcgcc
     4201 aggacagtcc tgaatcgcac gagcacggct tgcccaccga cgaagagctc gacgccatcg
     4261 agctccagca cgcgactcga atggtgcagt caggcgcgga ggtcacggca gacgaagagc
     4321 tcggggtctg gtggatccgg ttcgagcgtc ggacaccaca gctggcattt gccatgcgcc
     4381 tgcgctggcc agaggcggag gctgagacgc gcctggcgcg gttgactgcg gtgatgcagg
     4441 ggcgcggcga gtggccctcg ctgtcgatct ctgagggcct cacgcagccg accaccatcg
     4501 gcgacacgct tgccgacgcc ggttggcgga tcgtggccgg agagcgactc atgttcacgc
     4561 gtcacccacc cacgaacccg cacctcgacc ctacgctccg gatcgtggcc gttacgccgg
     4621 cgaccgcaaa ggagtgcgtc gagctggaga tcaccaattt cggcttgccg ctctccgagg
     4681 ttgagccgcg taccgagtgg ctggccgagg ccgttcgaaa cggctcagag cgcgcgtaca
     4741 tcgtcagatc catgcacaag cccgtcgcca gcgcgcggct gatccccggc gccggcggag
     4801 ctgcctgcct cagcgcgatc aacgttgtcg ctgcccaacg cggccgtggg tacggtcggc
     4861 tcgtgacgtc cgtggcgacg cgtgctggtc tcgcgaccgg cgcgaagctc gtctggctgt
     4921 ccgtcgacga gtcgaacgca ccggccgtga agctgtacga aagcctcggc tttgagccga
     4981 cgttcgcctg gtcccgctgg atagcgtcgg cttgacacga gccggcgccg cctcctgctg
     5041 gcgatcaacg tctagtcggc gtcgagctcc gcgatctcct cggctgatcg gtcgcgcgac
     5101 tggctgacgg ccatcgtgag actgaaggtc gccgaaccgc cggcctccaa tttgagcggc
     5161 cagcggaaca gcaggcacga gccctggtac atgcgctcga acccggcctc cgagttggac
     5221 accgtctcga tcggatacca gctggccgcg gcggcgggct cggcggaggc ggcaatatcg
     5281 acgccgtcgt actcattgcc gaagcgcagc gccgtcccgg gctcgaccga gccggcctcg
     5341 tcgtggcgca tctcgccgtc ggcccagcgg tagtacgccg acaggttgcg accgccgcct
     5401 gacaggttga tgttccactc cacctcgagc acgccgtgga actcggagcc cgacctcgaa
     5461 gcgacctcaa cgtccagtgt cagtcgtggg tccagccggt tgccacccag ccgcacggac
     5521 ttggtcacga tgaggtcatc tgtcgcccgc gccaggcggg ccgcgtcggc cccaacctcg
     5581 gccagcgtcc actcgccgtg cacgaagtcg ccaacctcgc tcccatcccg tgccaggact
     5641 cggacaagcc cgctccgccg ttcgtagttg tcgtacgtca aaaagcgcga caggtttgac
     5701 tccttctgcc gcatgtcttc gtgcggattg ctgagctcct cgggatcggc ggatttagtc
     5761 gtcccggctg cctcggccct gcgcagcttc tcgtgataag cctctggacg tcggcgtaac
     5821 accgcggcga gcgcgacgcg tgtcgcgcgc aagtcccacg acccgatgcc ggcgccttcg
     5881 gcaatatcca cgagcacgct ctgtccaggg gtgccaagca ggacctcatc ggcgccatcg
     5941 aggtcgaagt cggctatccc ggacgaagcc tcgcccaacc gcgccagatc gtccgcggcg
     6001 atgaggtggt ggtaggtcgc catccgcatg tggacgatgt agatgccgcc gaaaagcccg
     6061 tgccaatagc aatcgttcga ctgggcgcga tggaggtggt cggtcgccag gtccttgatc
     6121 ggcccatcgg tcatcgcttg gaccgcggat gagactccga gcatctgctt gtgcaggtcg
     6181 ttgatctcgc ggtagcgggc ctggaagttg cgccatagtc cgccgcgaac aaatggcgcc
     6241 cacggcttcg ccttctccgc cttctccgcc ttctcgacga catggtggaa ctcgagcgcg
     6301 cgttcaggcg gcaatgccca ttcggtcatc tcgacatatg agctcgtggg gatgtagatc
     6361 cggccgatcg atggctgtcc atccatccag tcagacgggc gcacggtcga tacccaggac
     6421 gagttcgttt cgagggcggt gaagcaccga tcgacccact gctcctcccc ccagcacaga
     6481 tcgtgggttc ccggccagcc gccgaacttc tcgccgtcgt cgcccatcat tccgaggcgc
     6541 ttgccgtcgg ccgtggcatt ctgccgcagg taaccgatca gatcgtcgac tggcttccag
     6601 gggatgtggt agcgcagccc cttctcggtc gtgaacacgg tcagccggcg tccctggtcg
     6661 tcagtcgtgt acgtgcccca catgtcctcg tcgtggactg ccgcgccgcg caggtggttg
     6721 tcgtcgacga tcgtgtactc gtatccggcc gaggcgaggt cgtacgggac cgacggttcc
     6781 cagacgcgct cggcgagcca tgcgccgacg ggcgcggccc cgaacaccct ttcgagttcg
     6841 gatcgcattc gcatcagctg gccgtggcga tcgcgctgcg gcagagagac caggatcggc
     6901 tcgtagaggc cgccacccag gatctcgacc tgaccgcggg ccacgagcgc gcggatccgc
     6961 tcgatggatt cgggcctctc cgacaccatc cattccagca gcggtccggt gtaatggagg
     7021 ccgactcgga tccgcgggtg gcgttccagg gcaccgatca tgggctcgta ggagcgctcg
     7081 tagttctcct cgaacaccca gccgaagttg ccgaccggct ggtggttgtg gaagacgagc
     7141 gcgagcgaga tccggtccgt catgcgcgta tctccgagat gtggggccga acggtcaggc
     7201 cgcggcctga cccgtcttga ccggctcgtc gcgcttacct gaccgacggc gccgcccccg
     7261 ttcggccgct gggccgtaca gccgctccac gtactcctgg agcatgcgcg tcgtggagaa
     7321 ctgccagatt gtgctcgcca tggatgcctt catgcgctcg atccagcgcg ctggtatgcc
     7381 gttcgcattg cgttcgtaaa acgtcggcac gatctcctgc tcgagcagcc gatagaggtc
     7441 ctgagcgtcg gcccaatcct gtgcgccctc gtcggggttt tgctcgcgac tgccgatcga
     7501 ccagccgttg tggccgtcgt agccctcgtc ccaccacccg tcacgcaccg agaagttgat
     7561 gacgccgttc atggccgcct tcatgccgct cgtgcccgaa gcctcgaggg gccggcgcgg
     7621 gttgttgagc catacgtcga cgccctgcac gaggtagcgg ccgacgcgga tgtcgtagtc
     7681 ctcgagcacg aacacgcggt tggtgaactt ggcgacccga ctcttgccaa agatgtcctg
     7741 gatgaccttc tgccccggac ggtcggcggg gtgcgccttg cccgcgaaga tgatctgtac
     7801 gggtcgatca acgttgttga ggatccgccc cagtcgctcc tcgtcgctga agatcagggc
     7861 cgcccgcttg tacgtggcga atcgccgcgc gaagccgatc gtcagcacgt ccggatcgag
     7921 ggcgtcggcg agcgactcca gcagggccgg cgcttcccca tggcgcgcga actggttctg
     7981 cagccggcgt cggcagaaga acgcgaactc cagcttctgg cgcttgtgcg cctcccagat
     8041 ctggttgtcg gcgagcttgc cgatgcgctc ccagaaaggg ctttcttcct tgtcgaggtt
     8101 tgtcaggacg ccgtcgagct gctcatagag acgacggaac gggtcgccga cccagctcgg
     8161 cgggtggacg ccattggtaa tcccgaggat cggctgctcg acgatgccca gccacgtggc
     8221 attagccgtc tcggcgtgga gctggctcac ggcgttggcg ccgttcgtgt c
//
LOCUS       DATLCO010000024         5482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4494158, whole genome shotgun sequence.
ACCESSION   DATLCO010000024 DATLCO010000000
VERSION     DATLCO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4494158"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1073)
                     /gene="pyrF"
                     /locus_tag="VMZ33_00645"
     CDS             complement(177..1073)
                     /gene="pyrF"
                     /locus_tag="VMZ33_00645"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HUR15766.1"
                     /translation="MTSAVSSSSLPRAAEMGTAADGSPSYLERLCGRVEIVGTTLCVG
                     IDPEPSALPAGFSRDVRGIEAFCRLLIESTAQHAAAYKANLAFFEAFGSAGLAALERI
                     RAAIPAGIPFVADAKRGDIGSTAARQAVALYDVLGSDAVTVSPYLGREAVEPLIGRRD
                     RFAYVLCRTSNGGAGEFQGLAVDGDPLYVHVARHVSEWSASGATVGLVVGATAPQELE
                     RIRSVAPTLPFLVPGVGAQGGEVIPVLQHGPVTAGQANETRGGGLLVNVSRGISSAAL
                     EGQDPGVALFAASQRWARVLRC"
     gene            complement(1030..2187)
                     /gene="secF"
                     /locus_tag="VMZ33_00650"
     CDS             complement(1030..2187)
                     /gene="secF"
                     /locus_tag="VMZ33_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HUR15767.1"
                     /translation="MPVFDLVGKRRWFYIFSALITIPGLIFILLTPFSNGQAGLKFSI
                     DFTGGTVWEIHFANGRPDPTEVHEVMLEQGLPGTVAITTAAEREYVLIRTERVSGLTE
                     QPAASPGPSAAPSGSPAASSASPSGSPSASGSPAPSASASPAPSASAEASVAPSPSAS
                     PGAGGPINLPTEGDLGELRFALEDRFGPIDEVRQEANIGPIISSEITQQAIILIILGS
                     AGILGWMTFRFQDFRMGAAAIVALLHDVLVVVGVFAIGGTFFGLEVDALFVTAMLTVI
                     GFSVHDTIVVFDRVRENRVRHAGESFSSIVNHSLLQTLGRSLNTSITVGIVLLALLLF
                     GSDAIRAFVFAMVIGIISGTYSSIFNASMILVSWHERDDERRQQQFATARG"
     gene            complement(2174..3511)
                     /gene="secD"
                     /locus_tag="VMZ33_00655"
     CDS             complement(2174..3511)
                     /gene="secD"
                     /locus_tag="VMZ33_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HUR15768.1"
                     /translation="MRRFLPALIVVVGLAAFAIDFLPLARPLSDPPTPIETRLGLDLQ
                     GGLRGEYRALPTESSPINTQTMADIREIIENRINQYGVAEPIVQTQGSDRIVVEIPGI
                     TNEADVRRLIGSTGRLDFIEVPPARSAEVVSGQRIPPDLRVVFSGDKLSTAAPGVDDV
                     GRRGVNFTLDEDGARIFADYTRQHVNEQFAIVLDGIVISAPVINTPITGGQGIISGGF
                     SSTAQVNELVTVLRYGALPNGIQEVSFSKISPTLGLNFLQQSLLAAAIGVGLIFLFML
                     LHYRLPGLVACLALLFYALVVYAIFRLVPVTLTLAGVAAFVLSIGMAVDANILIFERT
                     KEELRGGKTLVAAIEAGFNRAWNSIFDSNMSSIITAGILFYFGSPTIRGFALVLIIGV
                     LISMFTAITLSREVLRWVVRQPWARRASLFGVSEQEFIVASPRARSREAGASV"
     gene            complement(3552..5414)
                     /gene="uvrC"
                     /locus_tag="VMZ33_00660"
     CDS             complement(3552..5414)
                     /gene="uvrC"
                     /locus_tag="VMZ33_00660"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HUR15769.1"
                     /translation="MTPQLAATLAKLPKNPGVYLLKDDRGRVLYVGKAQNLRNRVRQY
                     WQASRGTQAPLRIEQAMDRVADVEYTMVDSVSEALLLEATLIKRHQPRFNVRLKDDKS
                     YPYIKVTLGDDYPRIERTRKLPKDGSRYFGPYASASSVDEAMNLIRRLFPFRTCTIEI
                     KEGKRALPRPCLLYHIKRCQGPCIQAISKQDYGSDIEQVMLFLEGRKDLVARRLREEM
                     AFASEATDYERAADMRDKLRAIERTMESQKMAAFARTELDVLGFARQGGGAAVQLFAI
                     RNGTTVARDIFLLENVGDSSDDEALSAFIKQYYAQASSIPPRVLLPKLPPDSDELTAF
                     LASRRGRKVVLAVPERGERRALMSLAAQNAAETLAREQAKWLADEGKTLAALEELGGS
                     LGLSAPPLRIECYDISTIQGTNTVGSMVVFEEGRPRTGEYRRFRVRTAEVAPGRPDDY
                     ASHREVLRRRFHRALEAEEGVAEELRWRLPDLVVIDGGLGQVNAARGVLDELGLLDMP
                     VIGLAKEREEIWLPGAPAPFVLPANSQALYMLQRLRDEAHRFAITYHRKLRAKAQTKS
                     ALDELPGVGPARKRALLRVFGSTKQMRSATVDEIASVPGISRSLAERIHEGFNA"
BASE COUNT          991 a         1768 c         1830 g          893 t
ORIGIN      
        1 ggtgtttcga gcctgagtga gtcttcgacg tcggtggagg ccttccggaa ctctctgatc
       61 gtgcgcccga gcgcgttccc gacttcaggc agcttgcccg gtccgaggat cagcaacgcc
      121 aggatcagga ccagcagcag ctcaaacggg ccgatgttga agggcatggc tcgatgctaa
      181 caccggagaa ctcgcgccca gcgctgggag gcagcgaaaa gagcgacgcc tggatcttga
      241 ccttcgagcg ccgccgagct aatgccacgc gacacgttca cgagcagtcc gccgccccgc
      301 gtttcgttgg cctgaccggc ggtgaccggg ccatgctgca ggacaggtat gacctcgcca
      361 ccctgagcgc cgacgcccgg cacgaggaac gggagggtcg gcgctacgga gcggatccgc
      421 tccagctcct gcggcgcggt ggcaccgaca acgagaccca cggtcgcgcc actcgccgac
      481 cactcggaga cgtgccgcgc gacgtggacg tacagcgggt ccccgtcaac cgccaacccc
      541 tggaactcgc ccgcgccacc gttggacgtc cggcacagta cgtacgcgaa ccgatcgcgg
      601 cgcccgatga gcggctccac ggcctcgcga cccaggtacg ggcttacggt cacggcgtcg
      661 gagccgagga cgtcatacag ggccacggct tgacgggcgg cggtcgagcc gatgtcacca
      721 cgtttggcgt cggcaacgaa cgggatgcct gccgggatcg ccgcccgtat ccgctccagc
      781 gcggcaagcc ccgcgctgcc gaaggcctcg aaaaacgcca ggttggcctt gtacgccgca
      841 gcgtgttggg cagtcgattc gatcagcagc cggcagaaag cctcgatacc acgaacgtca
      901 cggctgaaac cggcggggag agcagatggc tccggatcga tgccgacgca gagcgtcgtt
      961 ccaactattt cgacgcgtcc gcagagacgt tcgagatagc tcggcgaccc atcggccgcc
     1021 gtgcccatct cagccgcgcg cggtagcgaa ctgctgctga cggcgctcgt catctcgctc
     1081 gtgccagctc acgaggatca tgctcgcgtt gaagatcgac gagtacgtgc ccgagatgat
     1141 cccaataacc atcgcgaaga cgaaagcgcg tatggcgtca cttccgaaca ggagcaacgc
     1201 gaggagcacg atgccgacgg tgatcgacgt attcagcgag cggccaaggg tctgaagaag
     1261 gctgtggttg acgatcgacg agaaggactc gccggcgtgg cgaacgcggt tctcgcgcac
     1321 gcggtcgaac accacgatcg tgtcgtgtac cgagaacccg atgactgtca gcatggcggt
     1381 cacgaagagc gcgtccacct caaggccgaa gaacgtgccc ccgatggcga agaccccgac
     1441 gaccaccagc acgtcgtgca acagcgccac gatcgctgcc gcgcccatgc ggaaatcctg
     1501 gaagcggaac gtcatccagc cgaggatccc ggccgaaccg aggatgatca ggatgatggc
     1561 ctgctgcgtg atctcggagc tgatgatcgg gccgatattg gcttcctgac ggacctcgtc
     1621 gatcggtcca aagcggtcct cgagcgcgaa tcggagctca ccaaggtcgc cttctgttgg
     1681 caggttgatc ggcccacccg ccccgggcga cgccgaagga ctgggcgcaa ccgatgcttc
     1741 agccgaggcc gacggagctg gtgaagccga cgccgagggc gcaggtgacc ccgacgcgga
     1801 cggtgagccc gaaggcgatg ctgagctggc ggcgggcgag ccactgggag ccgcgctggg
     1861 gccagggcta gctgctggct gttccgtcag accggagacg cgttctgtcc gaatgagcac
     1921 gtactcacgc tcggcggcag tcgtaatcgc gacggtgccg ggcaggccct gttcgagcat
     1981 cacctcgtgc acttcggtcg gatccggccg gccgttcgcg aagtggatct cccagacggt
     2041 cccgcccgtg aagtcaatcg agaacttcag tccggcttgc ccgttcgaga agggcgtcag
     2101 caggatgaag atcagccctg ggatcgttat cagcgcgctg aagatgtaga accagcgacg
     2161 cttgcctacg aggtcaaaca ctggcacccg cttcacgaga tcgcgcgcgt gggctcgcga
     2221 cgatgaactc ctgttcggat accccgaaga gcgatgcgcg acgtgcccac ggctgccgta
     2281 cgacccagcg aaggacctcg cggctgagcg ttatcgccgt gaacatcgag atgagcacac
     2341 cgatgatcag cacaagcgcg aagccgcgga tcgtcgggga gccgaagtag aacaggatgc
     2401 cggcggtgat gatgctcgac atgttcgagt cgaagatgga gttccaggct cggttgaatc
     2461 cagcctcgat cgctgccacg agggtcttcc cgccgcggag ctcctccttg gttctctcga
     2521 agatgaggat gttggcgtcc accgccatgc cgatcgaaag gacgaaggcc gcgacgccag
     2581 cgagcgtcaa cgtcacgggc acgagacgga agatcgcgta cacgaccaac gcatagaaga
     2641 gcagtgcgag gcaggccaca agcccgggca ggcggtagtg caacaacatg aacaggaaaa
     2701 tgagcccaac gccgatggcc gcggcgagga ggctctgctg gagaaagttc aagccaagcg
     2761 tcggactgat cttgctgaag ctgacttcct ggatgccgtt cggcagcgcg ccgtagcgca
     2821 acaccgtgac cagctcattg acctgggcgg tagaagaaaa accgcccgaa atgatgccct
     2881 ggccgccggt gatgggcgta ttgatgacgg gggcggagat gacgatcccg tcgaggacga
     2941 tggcgaactg ctcgttcacg tgctggcgcg tgtagtcggc aaatatccgg gcgccgtcct
     3001 catcgagggt gaagttcacc ccacgccgtc cgacgtcatc tactccgggc gcggcggtac
     3061 tcagcttgtc gcccgagaac acgacacgca ggtccggggg tatgcgctgg ccagagacga
     3121 cttcggctga ccgcgccggc ggcacctcga tgaagtcgag ccgacccgtc gagccgatca
     3181 gccgccgaac atcagcctca ttggtgatgc ccgggatttc aacgactatt cggtcgctgc
     3241 cctgtgtctg cacaatcggc tcggccacgc cgtactggtt gatgcggttc tcgatgatct
     3301 cgcggatgtc ggccatcgtc tgggtgttga tcggcgagga ctccgtcggg agcgcgcggt
     3361 attcgccgcg caatccgccc tgcaggtcga gtcccaggcg ggtctcgatc ggggtcggcg
     3421 gatcgctcaa cgggcgggcg agcggcagga agtcgatcgc gaacgcggca aggccgacga
     3481 cgacgatcaa cgcgggaagg aaacgacgca tcggccgcga atgttagagc acgcgacgga
     3541 tgtcgctgcg cttaggcgtt aaagccttcg tggatccgct cggccagcga tcggctgatc
     3601 cccgggacgg acgcaatctc gtccacggtg gccgagcgca tctgcttggt cgagccgaat
     3661 acgcgcagca gcgcccgctt gcgcgccggc ccgacaccgg gcagctcatc cagggccgac
     3721 ttcgtctgtg ccttggcgcg cagcttgcgg tggtatgtga tggcgaacct gtgcgcctcg
     3781 tcgcgcagcc gctggagcat gtacagcgcc tgcgagttgg ccggaagaac gaacggggcc
     3841 ggcgcccccg gtagccagat ctcttcgcgc tccttggcaa ggccgatgac cggcatgtcg
     3901 agcaggccca gctcgtcgag gacgccacgc gcggcgttca cctggcccag gccgccatcg
     3961 ataacgacga ggtcgggaag gcgccagcgc agctcctcgg cgaccccctc ttcggcctcc
     4021 agcgcgcgat ggaaacgacg gcgcagcact tcgcggtggc tggcgtaatc gtcagggcgg
     4081 ccgggagcga cctctgccgt ccgcacccgg aagcgacggt attcacctgt ccgtggccgc
     4141 ccctcctcga atacgaccat gctgcctacc gtgttcgtgc cctggatcgt gctgatgtcg
     4201 tagcactcga tacgcaacgg cggcgcgctc aggcccagtg aaccgccgag ctcctcgagt
     4261 gcggcgaggg tcttgccctc gtcggccagc cacttggcct gctcgcgggc cagtgtctcg
     4321 gccgcgttct gcgccgcgag gctcatcagc gcccgtcgct cccctctttc aggcacggcc
     4381 aggacgacct tgcggccgcg gcgcgacgcg aggaaggcgg tcagctcgtc gctgtcgggt
     4441 ggcagcttgg gaagcagcac gcgcggcggg atcgagcttg cctgagcgta gtactgcttg
     4501 atgaacgccg agagcgcctc gtcgtcggac gagtcgccga cgttctccag caggaagatg
     4561 tcacgcgcga cggtcgtccc gttgcggatc gcgaagagct gcaccgcagc accgccgccc
     4621 tgtcgagcga agcccagcac gtcgagctcc gttcgggcga aggcggccat cttctggctt
     4681 tccatcgttc gctcgatggc gcgcagcttg tcgcgcatgt cggccgcccg ctcgtagtcg
     4741 gtcgcctccg aggcgaaggc catctcctcg cgcaggcgtc gggcgacgag gtcctttcgg
     4801 ccttcgagga aaagcatgac ctgctcgata tcggagccgt agtcctgctt cgagatggcc
     4861 tggatacatg ggccctgaca gcgcttgatg tggtacagga gacagggccg cggaagagcc
     4921 cgcttgccct ccttgatctc gattgtgcac gtccggaacg ggaacaggcg ccggatgagg
     4981 ttcatggcct catcgacgct gctcgccgac gcatacggcc cgaagtaccg gctgccgtcc
     5041 ttgggcagct tgcgagtccg ctcgatccgc gggtagtcgt cgccgagcgt gaccttgatg
     5101 taggggtagc tcttgtcgtc cttgagccgg acgttgaacc gtggctgatg gcgtttgatg
     5161 agcgtcgctt cgagcagaag cgcctcgctc acgctgtcca ccatcgtgta ctcgacgtcc
     5221 gccacgcggt ccatcgcctg ctcgatacgc agcggcgcct gcgtcccgcg gctggcctgc
     5281 caatactgcc tgacgcggtt ccgaaggttc tgcgccttgc cgacgtacag gactcggccg
     5341 cgatcgtcct tgaggagata gacgcccggg ttcttgggca gcttggcgag cgtcgccgct
     5401 agttgagggg tcatccgttc cgatcgtagc gggcaaagag aagcggcccc gcctttcgac
     5461 ggggccgctc tcacgacgtt cg
//
LOCUS       DATLCO010000025        11101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7464953, whole genome shotgun sequence.
ACCESSION   DATLCO010000025 DATLCO010000000
VERSION     DATLCO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7464953"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VMZ33_00665"
     CDS             <1..436
                     /locus_tag="VMZ33_00665"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUR15770.1"
                     /translation="MISVLMELTNAMFRGRGTDVVDSNEWDETCRLLLLMLAPIAPHI
                     SEELWSRRLAARGEEWVSIHQQRWPSFDASLVAETEIELPIQINGKLRDVVHMPAGLS
                     EIEIEQIVMSRDKVQAQLAGMEVVRVIHVRDRMVNVVVKPAR"
     gene            complement(433..558)
                     /locus_tag="VMZ33_00670"
     CDS             complement(433..558)
                     /locus_tag="VMZ33_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15771.1"
                     /translation="MAAGASLIAPPLETPWRSLNSRLETPAGLQITLFQELDSTD"
     gene            complement(779..1453)
                     /locus_tag="VMZ33_00675"
     CDS             complement(779..1453)
                     /locus_tag="VMZ33_00675"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HUR15772.1"
                     /translation="MSTATWHVDSHDDHARHGHQASLEDALDFVNTYEFDKGDGHEHL
                     TDIPTAVDWLQKHELLHREMVDPLIERYSEEAEDPVVLARIKKVRSSLRELLDATVDR
                     RPPDSIALREVNKALRAPYMIELVPAADGVSLDHRHEGDPIAGAMARLAEAVARELTQ
                     GDSKRMRICANDECRWVFRDYSPAGRRKWCDMSTCGNRAKAARHRERQRSKAGSPESA
                     SAEPVN"
     gene            complement(1592..1888)
                     /locus_tag="VMZ33_00680"
     CDS             complement(1592..1888)
                     /locus_tag="VMZ33_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15773.1"
                     /translation="MSRMIWLALRASWQRQPPINADSCRLAESELSTDVGAVGLALSR
                     LVPFARQELHLDVHVSGSKALSFVLSCIRPEAFSGGADQGYDGESDEDEEGFHS"
     gene            1956..2963
                     /locus_tag="VMZ33_00685"
     CDS             1956..2963
                     /locus_tag="VMZ33_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR15774.1"
                     /translation="MDDSRPINTDIRLGLLCWNQYTDWQSLLEVGQRADRLGFYSLWT
                     WDHLYPIVGSDRGPFFEGYLTLAAWAQSTSRIRVGLMVGANPFRYPTLTAKMATTLDH
                     ISGGRAYLGIGSAWNDDEAKAFGIEFGESPAERLRWLRESLPIMRAMLHGEEPSAEGP
                     RYFADAVRNDPPPVQAHLPILIGGGGEKVTLRLVARYGDANNIGFAAGLETFKRKEAA
                     LRRHCEEVGRDQSEIERTMNTGPIVIRDSTDDAVRELERIYEHNGGAQQWAGRAASEQ
                     PTGSPEHVVEVLLPFVEQGYRHLVIGSPSPYDVETMERLVSEVQPRLQAAVVASPVGV
                     A"
     gene            3140..3880
                     /locus_tag="VMZ33_00690"
     CDS             3140..3880
                     /locus_tag="VMZ33_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HUR15775.1"
                     /translation="MDRFGEWRPIQAAEDEPEKLPKKAEEPADKDGKSDQVRLYAALA
                     AVVLAVTGAVIWLTSTGQAAGSGNLAINAAAAFVPHDSARSPWATEGSAISGAAVVVD
                     VQGAVLLPGVHRLTAGARIADAIAAAGGYSTRVDIARAAAILNLAAPVADGAKVHVPS
                     LGDVTATVGEPALGPSPGSGASGLVNVNTATADDLDSLPGIGPVTAAKIIDARTEAPF
                     ASVEELLSREVLGASTFENLRELVSVGP"
     gene            3887..6451
                     /locus_tag="VMZ33_00695"
     CDS             3887..6451
                     /locus_tag="VMZ33_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HUR15776.1"
                     /translation="MPRAGWLAVGAVVGALVAGGGFPVVTLALVGMAASGTGWRLARR
                     QRWPTGLLALGIGAVLLRAALSLLLAGGASAGGGDAAIPGGAWDHEAAVVSVNAPQGG
                     SQRAIVELRAPDPPDRVWATLPRYPPIAPTDVIRFGGALEPAPIDDEFGAFLARSGIA
                     YTVRARSMERVGSDGSPLAALEGLRRGAALMITTALPEPQGGLAAAMSIGLRDLVSRD
                     VSDDFRAAGLSHVVAISGWHIAMLAAIIAGALGRLSRRKRTIVVIAVVSAYALFAGAS
                     PGILRAAVMAGVVLVARESGRTGSASAALGLTVAGMLVIDPNTISDLGFQLSAAATAG
                     LLTWGSRASEWFGKRLPKRTPTWLLESLGVSTAAQAATLPLVLFHFGTLSIVAPLANL
                     LMAPIVAPGMLVTAIAFVCGLAVTLGVPAIVTAPFVLTGSLLIGLMIEIARFCGRLPF
                     ANVVIEEPLNLVAAATVAVVIGMIVRRRRAPAATGPETFRAEESPRSGRRLAFAGAGA
                     AVCILVASVNAAQPDGRLHITVLDVGQGDAIFLRGPDGGRALIDTGPDPDRLTALLDE
                     RIPAWDRRLDLVVITHPHEDHIAGVAALLDRYRIGEVAEPGMIGPGPGDAAFRRRMAE
                     LGRDSRTLAAGDSVRFDGITLHVRWPLPGTVPLRPPDGGTGINNVSIVLDASYGARRI
                     VLAGDVEEDVDRHLLEAGLAADERRVDVLKVAHHGSGTATTDGFLEHTAPSVAVISAG
                     WRNPYGHPSPSTVARLEDAGAKVFRTDLDGTVDISTNGTDLVANAGGGRPRPATPPPP
                     TQPVGVGFCPIGPAVSGRRRRTYNRRNVHPITRGGGATPARPPAAGLAAWPLHGGG"
     gene            6444..6935
                     /locus_tag="VMZ33_00700"
     CDS             6444..6935
                     /locus_tag="VMZ33_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HUR15777.1"
                     /translation="MGEVASFLATRIAERGHAINVALVETAALLHDVDKALPPEDKRR
                     EHGHGYGGAIWAHDEGLDEIAAAIADHPVGRLMDDGAYLPWAAWATVEERVVAYADKR
                     AHQDVVSLAERFAEWIERHGASPEMDVARERANELERQICEAAGIEPADVQRNPWVAA
                     ASA"
     gene            6932..8080
                     /gene="holA"
                     /locus_tag="VMZ33_00705"
     CDS             6932..8080
                     /gene="holA"
                     /locus_tag="VMZ33_00705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HUR15778.1"
                     /translation="MSDQAMSVPYLGYFWGEDAYGVDHAAVLFAAEHAAAAGGQPLDV
                     WRTSVDGDADEGETGGGSSAKRRARVFEQMEERLATSPMFSGGTMCVVRQPAALLRES
                     SSRERLIGLLDGVAPGNVLVFVDLIASGAKGPAQQGALRDAISQRKGRVAEFPALSRE
                     RMEGWLVQRARELNVSFAQGAAHMLAERVGAYVREGDVDRRRQSELANAELEKLALYR
                     PTTPITTQDVEEMVSEAVPGSTWAFLDAVGTRRPGEAATLVERLLGDQAPLPVLITQI
                     HRRIRELVVVREHLDAATRPQDLVRELKVQPFRAQKLAEQARAWQADELDDALIQLYE
                     LDLLSKGIANDGSPRSLSEDRSELGLLVWLAEHVGRAGGTARPTATVR"
     gene            complement(8131..8388)
                     /locus_tag="VMZ33_00710"
     CDS             complement(8131..8388)
                     /locus_tag="VMZ33_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15779.1"
                     /translation="MARIGNACPVCSRSLVSSRFDATFRLPEGDERLFFGIPASLCQV
                     CQQLYLDADLIDLLEVPEGRCTFAIESDQVLTREAWPGPAY"
     gene            complement(8496..8948)
                     /gene="rsfS"
                     /locus_tag="VMZ33_00715"
     CDS             complement(8496..8948)
                     /gene="rsfS"
                     /locus_tag="VMZ33_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HUR15780.1"
                     /translation="MTTDELPEPTPIRAGLPKRRTPVRKKQIEKGEKPAEEQALELAR
                     RIVDLASDKKASDIVLLEVGAITTLADYFVICSAQSERQLGAIADGIVAGLRDEGTKP
                     IGREGAANAHWLLVDYGSVIVHVMAPLERDFYQLERLWADAPLLLRLQ"
     gene            complement(8945..9553)
                     /gene="nadD"
                     /locus_tag="VMZ33_00720"
     CDS             complement(8945..9553)
                     /gene="nadD"
                     /locus_tag="VMZ33_00720"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HUR15781.1"
                     /translation="MSERWGILGGIFDPIHYGHLTVAEQTRDALALDTVLFIPTGIPV
                     HKHAPDASAEDRARMVEIATADNPAFQVSRIELDSDRPNYTVDTLQALTDETPAREFT
                     LIVSSETASYMPEWHLPERILQLARVAIVSRLGYADISLEWISHHFPEQADRFVRVAT
                     SHLGHSSTDIRDRVAGGRPIRYLVPAAVEAYIGDNHLYGYGQ"
     gene            complement(9570..10844)
                     /gene="obgE"
                     /locus_tag="VMZ33_00725"
     CDS             complement(9570..10844)
                     /gene="obgE"
                     /locus_tag="VMZ33_00725"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HUR15782.1"
                     /translation="MAFLDEVTITVRGGDGGHGASSFRREAHVPRGGPDGGDGGRGGS
                     VYLRVDSGETMLRDYRYKHHFRGESGGSGSGSRKHGKAGKDLDLNVPPGTVVRDLETN
                     DLIADLVSREQRVMIARGGRGGLGNTHFATSTNQAPTHAQKGEPGEERKLKLELRLIA
                     DVGLVGLPNAGKSTTLSALTAATPQVAPYPFTTLEPNLGVLELEDERRVTIADMPGLI
                     EGASAGAGLGHAFLRHVSRNRVLAHVVDLSTVDPERDYQVIREELEAHDASLLSKVTL
                     VIGNKIDLDEATAAAKAFAKSRRRDGIEFVAVSAALGEGVDELLEALGRLLPDAETLA
                     EPGEPAGVVIHRFENAPDMFTVSREGDAYRVMGRRIERLAAQTDFENEESAERFQRDL
                     ARSGIERELVRAGVGQGDTVRIGQVELEWDPD"
BASE COUNT         1762 a         3654 c         3799 g         1886 t
ORIGIN      
        1 catgatctcc gtgctcatgg agctgaccaa cgcgatgttc cgcggccgcg gcacggacgt
       61 cgtcgattcg aacgagtggg acgagacgtg ccggttgctg ctgctgatgc tcgcgcccat
      121 cgcgccgcac atcagcgagg agttgtggtc acgtcgactc gctgcgcgcg gcgaggagtg
      181 ggtgtccatc caccagcaac gctggccgtc gttcgacgcc tcgctcgtgg ccgagacaga
      241 gatcgagctg cccatccaga tcaatggaaa gctgcgcgac gttgtccaca tgcccgcggg
      301 gctgtcagag atcgagatcg aacaaattgt tatgtcacgc gacaaagtcc aggcccagct
      361 cgccggcatg gaggtcgtgc gcgtgatcca tgtccgcgac cggatggtca acgtggtggt
      421 caaacccgcg cgttagtcgg ttgagtcgag ttcctgaaag agcgtgatct gcaggccggc
      481 gggcgtctcc agccgcgagt tgagcgatcg ccacggcgtc tcaagcggcg gcgcgatcag
      541 cgaggcgccg gcggccacca gcgcgtcggt cgtcgcgggt ccatcggcga cctcgaacgc
      601 gacgcggtac ttgggtgcga cctgacggcc gacctccagc tcgtcgatgt agcgcttctg
      661 ggctgaattt acgtgaggcc cagaacgtcg cggtagaagt caatggccgc gtcgtagtcc
      721 tccgcctcga ccacgaggcg catctgggcg acgcgtcgcc ttggccgcgc gtcggccact
      781 agttgactgg ctcggccgat gccgactcgg gcgaaccggc ctttgacctt tgccgttcgc
      841 ggtggcgcgc ggccttcgcg cggttgccgc acgtgctcat gtcgcaccac ttgcgacgcc
      901 cggcgggcga gtagtcgcgg aacacccagc ggcactcgtc attggcgcag attcgcatgc
      961 gctttgagtc accctgggtg agctcccgcg cgaccgcctc ggcgagtcgc gccatggcgc
     1021 cggcgatcgg gtcaccctcg tggcgatggt cgagcgagac gccgtcggcc gcgggcacga
     1081 gctcgatcat gtacggcgcc cgcagcgcct tgttcacctc acgcagggcg atcgaatcgg
     1141 gcggtcgtcg atcgacagtg gcgtccagca gctcccgcag cgagctccgc accttcttga
     1201 tgcgggcgag caccaccggg tcctcagcct cctcggaata gcgctcgata agcgggtcca
     1261 ccatctcgcg gtggagcagc tcgtgcttct ggagccaatc aacagcagtt ggaatgtcgg
     1321 tcaggtgctc gtgtccgtcg cccttgtcga actcgtacgt gttgacgaag tcgagcgcat
     1381 cctcaagcga tgcctgatgg ccgtggcgag cgtgatcgtc atgtgaatcg acgtgccagg
     1441 tggccgtcga catgccgttt gcgacgccgg acggcgccgt ttcagcggtt accgaagatt
     1501 ccattgcgta accagttgta catcattgac aggttactcg caagtggcta ttctacgggt
     1561 gttgagtaac cgtatagaaa ccgataagga gttacgaatg gaagccttcc tcatcctcgt
     1621 cgctttcgcc gtcgtagccc tgatcggcgc cgccgctgaa agcttcggga cggatacacg
     1681 agagaacaaa actaagagcc ttggagcctg aaacatgtac atcgagatgg agctcctggc
     1741 gcgcgaacgg cacgagtcgc gacagcgcga ggccgacggc tcccacatcc gtcgacagct
     1801 cgctttcggc cagcctacaa ctgtccgcgt taataggcgg ctgtcgctgc cacgacgccc
     1861 gcagcgctaa ccagatcatt cgcgacaagc gagtcacgcg acccgccgga ccctccctcc
     1921 ggcgggtcgt ttgattcccg gctaggctcg ggttcatgga tgactcgcgc cccataaaca
     1981 ccgatatccg actgggcctc ctgtgttgga accagtacac cgattggcag agcctgctcg
     2041 aggtcggcca gagggccgat cgcctgggct tctacagcct ctggacgtgg gaccacctct
     2101 acccgatcgt cggctccgac cgcgggccgt tcttcgaggg ctacctgacg cttgccgcct
     2161 gggcgcagtc gacctcgcgc atccgtgtcg ggctcatggt cggggccaac ccgttccgct
     2221 atccgacgct cacggccaag atggccacaa ccctcgacca catctctggt gggcgggcgt
     2281 acctcggtat cgggtcagca tggaacgacg acgaggccaa ggcgttcggt atcgagttcg
     2341 gtgaatcccc tgctgagcgg ctgcgctggc tgcgcgagtc tctgccgatc atgcgcgcca
     2401 tgctccacgg cgaggagccg tccgcggagg ggccacggta cttcgctgat gcggttcgga
     2461 acgatccgcc gcccgtccag gcgcacctgc cgatcctcat tggtggtggt ggcgagaagg
     2521 tgacgctgcg gcttgtcgcg cggtacggcg atgccaacaa cattgggttt gccgcggggc
     2581 ttgagacctt caagcgcaag gaggccgcgt tgcggcggca ctgcgaggaa gtgggccggg
     2641 atcagagcga gatcgagcgg acgatgaaca ccgggccgat cgtgatccgc gactcgaccg
     2701 atgatgctgt gcgcgagctc gagcggatct acgagcacaa cgggggtgcc cagcagtggg
     2761 ccggccgggc agcgtcggag cagcccacag gatcgcccga acacgtcgtc gaggtcctgc
     2821 tgcccttcgt cgagcagggc tatcggcatc tagtcatcgg gtcgccgtcg ccgtacgacg
     2881 tggagacgat ggagcgcctc gtgagcgagg tccagccccg gctccaggcc gccgtcgtgg
     2941 cgtcgcccgt aggagtggcg tagccggctc ttccctcctc cggaggtcgt ggataactgc
     3001 ccttcgtagc gataagcgcc cgataagagt cggttcgtaa ggtcccgtct gcgctcgccg
     3061 atggcccgaa gtgccccggg tcgttggcgg gcgaccttgc atccgccgat gagtcgacct
     3121 cccgccgtgg agggtttcga tggatcggtt tggagaatgg cgcccgatac aagcggcgga
     3181 ggacgagccg gaaaagctcc cgaagaaggc agaagagccg gccgacaagg acggcaagtc
     3241 ggaccaggtg cggttgtacg cagctcttgc cgccgttgtc ctggcggtaa cgggcgcggt
     3301 catctggctg acgtcgaccg gccaagccgc gggtagcggc aaccttgcga tcaacgctgc
     3361 ggcggcattc gtgccgcatg actccgcccg atctccgtgg gcgacggaag gcagtgcgat
     3421 ttccggggcg gccgtcgtgg ttgatgtcca gggcgcggtc ctgctgcccg gtgtgcaccg
     3481 cctgactgcc ggcgcgcgga tcgccgacgc aatcgcggcg gctggcggct actcgacccg
     3541 agtcgacatc gcacgagcgg cggccatcct gaatttggcc gcgccggtgg cggacggcgc
     3601 caaggtgcac gtgccctcac tcggcgatgt gacggcgaca gtcggcgagc cggcgcttgg
     3661 cccgagtccc ggttcgggcg cgtccgggct cgtcaatgtc aacacggcga cggctgacga
     3721 tctcgattcc ctgcccggaa tcgggccggt cacggcggcg aagatcatcg acgcccgcac
     3781 tgaagcaccg ttcgcctctg tcgaagagct gctttcgcgc gaggtgctcg gagcctcgac
     3841 cttcgagaat ttgcgcgagc tggtcagcgt cgggccgtag cgagtcatgc cgcgcgcagg
     3901 ctggctggct gtcggcgccg ttgtcggggc actcgttgcc ggaggcggct ttccggtggt
     3961 gaccctggcg ctcgtcggaa tggcggcgag cggaaccgga tggcggcttg cgcgaaggca
     4021 acgatggcca accggcctgc tggctctcgg gatcggggca gtgctcctgc gcgcagcact
     4081 ctcactcttg ctggctggcg gggctagcgc cggcggagga gatgccgcca tccccggcgg
     4141 cgcctgggat cacgaagcgg cggtcgtttc ggtcaatgcg ccgcagggcg ggtcgcagcg
     4201 ggccatcgtc gagttgcgcg cgccggaccc gccggaccgc gtgtgggcga cgttgccgcg
     4261 ttatcccccg atcgcgccga cagacgtaat tcgcttcggc ggcgcactcg agccggcgcc
     4321 gatcgacgac gagttcggcg cgttcctcgc acgaagcggg atcgcgtaca ccgtgcgcgc
     4381 tcgttcgatg gagcgtgtcg ggtctgatgg ttcaccgctg gcggcgctgg agggcctccg
     4441 acgcggcgca gcattgatga tcaccaccgc cctgccagag cctcagggtg gtctcgcggc
     4501 ggcgatgagc atcggcctgc gcgaccttgt gagtcgtgac gtgagtgacg acttccgagc
     4561 cgccgggctc agccacgtcg tggcgatcag cggctggcac attgcgatgc tcgcggcgat
     4621 cattgcgggg gctcttggtc ggctcagccg gcgcaaacgc accatcgtcg tgatcgcggt
     4681 cgtctcggcc tacgcgctct tcgccggcgc gtcgccgggc atcttgcgtg cggccgtgat
     4741 ggcgggcgtc gttcttgtcg cgcgagaatc gggccgcact gggtcggcgt cggcggcgct
     4801 cgggctaacg gtcgctggca tgctggtgat tgaccccaac accatcagcg accttggatt
     4861 tcagctgtct gcagctgcga ctgccggtct gctgacgtgg ggcagccgcg ccagcgaatg
     4921 gttcggcaag cggttgccaa agcggacgcc gacgtggctg ctcgaatcgc tcggcgtctc
     4981 gacggcggcg caggcggcga ccctaccgct ggtcctgttc cacttcggaa ctttatcgat
     5041 cgtggcgccg ttggcgaacc tgctgatggc gccgatcgtg gcaccgggga tgctcgtaac
     5101 cgccatcgct ttcgtgtgcg gactagcagt cacccttggc gtgccggcga tcgtgaccgc
     5161 gcctttcgtt ctcaccggct cgctgctgat cggcctgatg atcgaaatcg cccgcttctg
     5221 cggccggttg ccgttcgcca acgtggtgat cgaggaaccg ctgaatctgg tcgcggctgc
     5281 gacggtcgcg gtcgtgatcg gcatgattgt tcgccgccga cgcgcacccg ccgcgaccgg
     5341 cccagaaacg tttcgcgccg aggagtcgcc ccgttccgga cgacgcctgg ccttcgccgg
     5401 agcgggcgca gctgtatgca ttctcgtggc cagcgtcaac gccgctcaac ccgacggccg
     5461 cctgcacatc accgtcctcg atgtcggaca gggcgacgcg atcttcctcc gcgggcccga
     5521 cggcgggcgc gcgctgatcg acaccggccc tgaccctgac cgcctcaccg cgctgctgga
     5581 cgagcgcatc ccggcgtggg atcgaaggct cgacctggtc gtgatcactc acccccacga
     5641 ggaccacatc gcgggcgtcg ccgcgctact tgatcgctat cgcatcggtg aggttgccga
     5701 accggggatg atcggccccg gcccgggtga cgcagccttc cgccgtcgca tggccgagct
     5761 gggcagggac agtcgaaccc tcgccgcggg cgactccgtc cggttcgatg gcatcacgct
     5821 tcacgttcgc tggccgctgc cagggacggt gccactccgc ccgcctgacg gcggtacggg
     5881 gatcaacaac gtctcgattg ttctcgacgc cagctacggt gcgcggcgga tcgtgctggc
     5941 gggtgacgtc gaggaagacg tcgaccggca cctcctcgag gcgggcctgg ccgcggacga
     6001 gcggcgcgtc gacgtcctta aggtcgcaca tcacggcagc ggaacggcca ccaccgacgg
     6061 tttcctcgaa cacaccgcgc cgagcgtcgc agtgatcagc gccggctggc gcaacccgta
     6121 tggccacccc tccccaagca ccgttgcgcg actcgaggac gccggcgcca aggtgttccg
     6181 gaccgacctc gacggcacgg tggacatcag cacgaacggc accgacctcg tcgcgaacgc
     6241 cggcggcgga cgtccgcggc ctgcgacgcc tccaccaccc acccaaccgg tcggcgtcgg
     6301 attctgcccg atcggcccgg cggtcagcgg tcgccgtcgg cgtacctaca atcgccggaa
     6361 tgtccatccc atcacgcgcg gaggcggcgc gactcctgcg cgacctccag ccgccggact
     6421 ggctgcttgg ccactccacg gcggtgggtg aagtagccag cttcctggcg acgcggattg
     6481 cagagcgcgg ccacgcgatc aatgtcgcgc tcgtcgagac cgccgcgctt ctgcatgacg
     6541 ttgacaaggc gctgcccccc gaagacaaac ggcgagagca tggccacggc tacggcggcg
     6601 cgatctgggc gcacgacgag ggcttggacg agatcgcggc ggccattgct gatcaccccg
     6661 tcggcaggct catggacgac ggcgcgtatc tgccgtgggc cgcctgggca acggtcgagg
     6721 agcgggtcgt cgcctacgcc gacaagcggg cacaccagga cgtggtctcg ctcgcggagc
     6781 gcttcgccga gtggatcgaa cgtcacggcg catccccgga aatggacgtc gcgcgcgagc
     6841 gtgcaaacga gctcgagcgc cagatctgcg aggcggcagg catcgagccg gctgacgttc
     6901 agcgcaatcc atgggtcgct gcggccagcg catgagcgac caggccatgt ccgtgccgta
     6961 tctcggctac ttctggggcg aagacgcgta tggcgtcgac cacgccgcgg tgctcttcgc
     7021 cgccgagcac gcggccgcgg ccggtggtca gccactcgac gtgtggcgga cgagcgttga
     7081 cggcgatgcg gatgagggcg agaccggcgg cggcagttcg gccaagcggc gcgcgcgcgt
     7141 cttcgagcag atggaggagc gcctcgcgac ctcgccaatg ttcagcggcg gcacgatgtg
     7201 cgtcgtccgt cagccggcgg cacttctccg cgaatcgagc tcccgcgaaa ggctcatcgg
     7261 cctcctcgac ggggtcgccc caggaaacgt gctggtgttc gtcgatctca tcgcgtccgg
     7321 cgcgaagggt cccgcgcagc aaggcgcgct gagggatgcg attagccagc ggaagggccg
     7381 cgtggccgag ttcccggcgc tgtcgaggga gcgcatggag ggctggctgg tccagcgcgc
     7441 aagggagctg aacgtcagct tcgcccaagg cgccgcgcac atgctcgcgg aacgtgtcgg
     7501 cgcctatgtc cgcgaggggg acgtcgaccg tcgccgccag agcgaacttg ccaacgccga
     7561 gctcgagaag ctggcgcttt atcgaccgac cacccccatc actactcaag acgtcgagga
     7621 gatggtcagc gaggcagtcc ccggctcaac atgggcgttc ctggatgccg tcgggacgcg
     7681 ccgcccggga gaggcggcca cgctcgtcga gcgactcctc ggagaccagg cgccactccc
     7741 cgttctcatc acccaaatcc atcgccgcat ccgggagctg gttgtggtcc gcgagcacct
     7801 tgatgccgcc accaggcctc aggatctcgt tcgcgagctc aaggtccagc cgttccgtgc
     7861 ccagaagctg gccgagcagg cgcgcgcctg gcaggcggac gagctcgatg acgccctgat
     7921 ccagctctac gagctcgatc tgctcagcaa agggatcgcc aacgacggct cgcctcgttc
     7981 actctcggaa gatcgctctg agctgggcct gctcgtctgg ctggcggagc acgtcggccg
     8041 cgcaggaggc acggcgcggc cgacggcaac ggttcggtag cgggctccgg tggaggacga
     8101 agcccgcccc gagtaagtga gcgggaagga ttagtaggct ggcccgggcc aggcctcacg
     8161 ggtgagaacc tggtccgact cgatggcgaa tgtgcaacga ccctccggaa cctccaggag
     8221 atcgatcaga tctgcatcca gatacagctg ctggcagacc tggcacaggc tcgcggggat
     8281 cccgaagaac agccgctcgt cgccctccgg cagccgaaag gtcgcgtcga agcgcgagct
     8341 gacgagactg cgagagcaga ccggacaagc gttaccgatg cgagccatgc ggggactcta
     8401 ggtgcgctca ggtgcggtcc tgcatgaccc cgtggtacct gtttctacgc cgtcggtcct
     8461 accccggcag acgtggatgt gcggttcgcg tagggctact ggagtcgaag aagaagcggg
     8521 gcgtcggccc agagccgttc gagctggtag aagtcgcgct cgagcggcgc catgacatgc
     8581 acgatgacgg acccgtagtc gactagcagc cagtgcgcgt tggccgctcc ctcgcgtccg
     8641 atgggcttgg tgccctcgtc gcgcaggccg gcgactatcc cgtcggcgat cgcgccgagc
     8701 tgcctctccg actgtgccga gcagatcacg aagtagtcgg ccagtgtggt gatcgctccg
     8761 acttcgagca gcacgatgtc ggatgccttt ttgtccgacg ccaggtcgac tatccgccgc
     8821 gcgagctcca atgcctgctc ctcggctggc ttttcgccct tctcgatctg tttcttcctc
     8881 actggcgtac ggcgcttggg caggccagcg cggatcggtg tcggctcagg cagctcgtcg
     8941 gtcgtcactg tccatatcca taaaggtggt tgtctcctat gtaggcttcc acagccgcgg
     9001 gcaccaggta gcggatcggt ctgccgccgg cgacgcgatc ccttatgtct gtcgatgaat
     9061 ggccgaggtg gctggtcgcg acgcgaacga agcggtccgc ctgctccggg aagtggtgtg
     9121 agatccactc gaggctgatg tcggcataac ccagccggct gacgatcgcc acgcgcgcca
     9181 gctgcagtat ccgctccggc aggtgccact ccggcatgta cgaagccgtt tcggacgaaa
     9241 caatcagcgt gaactcgcgc gcgggggtct cgtcggtgag ggcctgcaac gtgtcgacgg
     9301 tgtagttcgg acggtccgag tcgagctcga tccggctcac ctgaaaggct gggttgtcgg
     9361 cagtggcgat ctcaaccatg cgtgcccggt cctctgcgga ggcatcaggc gcgtgcttgt
     9421 ggacggggat gccggtcgga atgaagagca cggtgtccag cgccagggcg tcgcgcgtct
     9481 gttcggcgac ggtcaggtgg ccatagtgga tcgggtcgaa gatgccgccg agtatgcccc
     9541 agcgctcgct caagtcgctg cgcctcggtt caatcaggat cccactccag ctccacctga
     9601 ccgatacgca cggtgtcgcc ctgccccacg ccggcacgga caagctcccg ctcaataccc
     9661 gagcgtgcca ggtcccgctg gaagcgctcg gccgattcct cgttctcgaa atcggtctgg
     9721 gcggcgaggc gctcgatgcg gcggcccatg acgcgatagg cgtcgccttc gcggctgacg
     9781 gtgaacatgt ccggggcgtt ctcgaagcgg tggatgacca cgccggcggg ctcacccggc
     9841 tcggcgagtg tctcggcgtc ggggagcaaa cggccgagag cctcgagcag ctcgtcgacc
     9901 ccctcgccca gggcggctga gacggccacg aactcaatgc cgtcgcggcg ccgactcttg
     9961 gcgaacgcct tggcggcggc cgtggcttca tccaaatcaa tcttgttgcc gatgacgagc
    10021 gtgaccttgc tcagcaagga cgcgtcgtgc gcctcgagct cttcgcggat gacctggtag
    10081 tcgcgctcgg gatcaaccgt ggagaggtcg acgacgtggg ccagtacgcg gttgcgcgac
    10141 acgtgtcgca gaaatgcgtg gccgaggccg gcaccggcgc tggcaccctc gatgaggccc
    10201 ggcatgtcgg cgatggtgac acgacgctcg tcttccagct caaggacgcc caggttgggc
    10261 tcgagcgtgg tgaacgggta cggggctacc tgcggcgtcg cggccgtcag cgcggaaagc
    10321 gtggtcgact ttccggcgtt cggcagtccg acaaggccaa catcggcgat cagccgcagc
    10381 tccagcttca gcttgcgctc ctcgcccggc tcgccttttt gggcgtgcgt tggcgcctgg
    10441 ttcgtggacg tagcgaaatg cgtgttgccg agtcctcctc gaccaccgcg agcgatcatg
    10501 acgcgttgtt ctcgtgagac gaggtcggca atcaggtcgt tggtctcaag gtcgcgcacg
    10561 acggttccgg gtgggacgtt caaatccagg tccttgcccg ccttgccgtg cttccgggag
    10621 ccggagccac taccgccgga ttcgccccga aagtgatgct tgtaacggta gtcgcggagc
    10681 atggtctcgc ccgaatcgac gcggagatac acgctgccgc cgcggccgcc atcgccacca
    10741 tcgggaccgc cgcgcggcac gtgcgcttca cgtcgaaagg acgacgcacc gtgcccgcca
    10801 tcgccgccgc ggacggtgat cgttacttcg tcaagaaatg ccatgggtca ggaatcgtag
    10861 ccgtgccggg cagtagccca gcaaggaccg ccggagctgt cccatcgagc gactcacgag
    10921 cgccgggagg tgcccccggc ggacgtcagg cctcgtttgc ggccgcgctc ttggtcagcg
    10981 acgccaggaa ctccgcgttt gaggccgact tcgaaagccg gttgagcagc agctcgatgc
    11041 cctcgttcgt gcccacggcg gacagcatgc ggcgcatcgt ccagaccatg cgcaacgtgc
    11101 c
//
LOCUS       DATLCO010000026         4991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5243018, whole genome shotgun sequence.
ACCESSION   DATLCO010000026 DATLCO010000000
VERSION     DATLCO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5243018"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VMZ33_00730"
     CDS             <1..265
                     /locus_tag="VMZ33_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15783.1"
                     /translation="DFSDELRSNLGEVISLLPLPLTEATVESVDMGGKSYAVVLRLVG
                     ESETVLLQTRWKDRDGTPTVVEVSHIVEQGEAVQGDEEAAESD"
     gene            complement(271..2058)
                     /locus_tag="VMZ33_00735"
     CDS             complement(271..2058)
                     /locus_tag="VMZ33_00735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUR15784.1"
                     /translation="MAKSTKPKTNEIPKSKRGSPPKSVDGFGQLGLDDRLLQTLAELG
                     YEEPTPIQREAIPFLLEGQDILAEAPTGTGKTAAFALPLLQRIAANDRGKGKSADGHI
                     AALILVPTRELAMQVSEAIHRYGEKLSIRVLPVYGGQAIGLQLRALDRGVDVVVATPG
                     RAVDHVKRRSIHLEGVQMVVLDEADEMLDMGFAEDLETLLSATPDTRQTALLSATISA
                     PIARIAARHLRDPQRVEVHAEDRVGDGIARVRQVAYVVRRADKLAALGRILDVEDPTS
                     ALVFARTRGEVEELAERLGGRGHDAAALHGGMDQAQRDRIMNRFRDGDLDVLVATDVA
                     ARGLDIEHVSHVVNYDVPSNPDAYVHRTGRTGRAGREGVAITLVEPRESRLLKNIERT
                     INTRLELAQVPSTADLRERRLELLRANLRETLVEDGIDRYRVVVESLAAEFDVVEVAL
                     AAVSMADKASGRDGADETEIPTISLTPPLDRRVGGPPQKGGRYGGKDGGSPARGKAAR
                     GRGPDGGGAWVRLFVGGGRRMGIRPADLVGAITHEAGVSGASVGAIQIADSYSLVEVP
                     DATADDIARVLGSAKIKGRRIEVRRYRER"
     gene            2070..2705
                     /locus_tag="VMZ33_00740"
     CDS             2070..2705
                     /locus_tag="VMZ33_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HUR15785.1"
                     /translation="MTCGQLQAVRGTGQRRIGVVKVTSMGKVIYLLNVSLDGYVETAS
                     KSLDWALVDEELHGWFNDQMRSTDASLYGRRLYELMSGHWPHAGSDPNSTPVEKEFGE
                     LWLATPKIVFSSTLESVDWNSRLVRGSPAEELARVREEFPGDLAVGGATLASSFIRAG
                     LVEEFRLVVHPVVIGGGTPFFPPTDSPIPLRLTDTHTFSSGATYRAYSKID"
     gene            2755..3258
                     /locus_tag="VMZ33_00745"
     CDS             2755..3258
                     /locus_tag="VMZ33_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15786.1"
                     /translation="MDIALVLNLITTVAIVVGLAFGLFEVRQALRARRDLAAVDIVRT
                     VQTQEIRRAMTRILSLPDAVAPEVVRADQALLDAAVAVDSACEMWGSMVFEGIVELHM
                     LDRMVGGWVRGTWVRLEPWITQERIDKRTPSVAEWWQWLYEMLEADPDPGKKPGVHVS
                     YRGRIRN"
     gene            3372..3785
                     /locus_tag="VMZ33_00750"
     CDS             3372..3785
                     /locus_tag="VMZ33_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15787.1"
                     /translation="MKPFLAGAAAFALMGAVFAGVSMAAGPVTAQAPNARLALVVDGG
                     STNGSFTVLRKKGVTSVANPYVGVFCVTPSATLALGRIVPMVGVDLGGSPFADSGAAW
                     NRQLVYCPPGTIEVETFQVSTGAHRNDVGFTLTVF"
     gene            complement(3825..4637)
                     /locus_tag="VMZ33_00755"
     CDS             complement(3825..4637)
                     /locus_tag="VMZ33_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HUR15788.1"
                     /translation="MTAKAATATTLKTVDRALSVLEIVAADAPMPVRKLAHALGHNVS
                     STYHLVNTLVARGYLAKDSAGELRIGGRVGMLYAALTRSSDLARQMRPIVERLAAECG
                     ETVYLTHLIADRVVIEIVIESAQSLRVTGLAVGYSGSEDRRASGRVVMAFLGQNDLEL
                     VLDHAHLGLSAEEKTARRDQLFEKLPAIRENGFAFDDQMFEAGILGVAAPFFDAHGDV
                     LGSVTVTAPTVRREQLLGPVKEQVLKAADDVSAELHAPMSARRAAHAGRARA"
     gene            4744..>4991
                     /locus_tag="VMZ33_00760"
     CDS             4744..>4991
                     /locus_tag="VMZ33_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15789.1"
                     /translation="MTADLNDRSWWNEYRDELNRDKEWGSAARFFPARIEFTHGDGSW
                     TIDTRDGVAVSVTEGAHPLGSDLVIGGPDEEWQRVLNG"
BASE COUNT          801 a         1611 c         1668 g          911 t
ORIGIN      
        1 ggacttcagc gacgagctgc gctccaacct gggcgaggtg atctccctgc tgccgctgcc
       61 cctcaccgaa gccacggtcg aatcggtcga catgggcggc aagagctatg ccgtcgtcct
      121 gcgactggtc ggcgagagcg aaacggtcct tctccaaacg cgctggaagg accgcgacgg
      181 gacccccacg gtcgtggagg tcagccacat cgtcgagcag ggcgaagctg tgcaaggaga
      241 cgaagaagcg gccgagtccg actagcgcgg tcagcgctcg cggtagcggc ggacctcgat
      301 tcgtcggccc ttgatcttcg ctgatccgag cacgcgagcg atgtcgtccg cggtcgcgtc
      361 ggggacttcg acgagcgagt agctgtctgc gatctggatc gcaccaacgg acgctcccga
      421 aacgcccgct tcgtgggtga tcgcaccgac caggtcggcg ggccgaatgc ccatccgccg
      481 gcctccgcca acgaagagtc gcacccacgc tccaccgcca tcggggcccc ggccgcgcgc
      541 cgccttcccg cgtgcaggtg aacctccgtc cttgccgccg tacctgccgc ccttctgggg
      601 cgggccgcca acgcgtcggt cgagtggcgg ggtaagactg atcgtcggta tctccgtttc
      661 gtcggcgcca tcgcgtcccg atgccttgtc ggccatgctc acggcggcga gagcgacctc
      721 aacgacgtcg aactccgcag ccaggctctc aacgacgacc cggtagcggt cgatgccgtc
      781 ttcgacgagc gtctcgcgta ggttcgcgcg caggagctcc aagcgtcgct cgcggagatc
      841 ggccgttgac ggcacctgcg ccagctcgag cctggtgttg atcgtccgct cgatgttttt
      901 caggagccgg ctctcgcgcg gctcgacgag cgttatcgcg acgccctcac gacccgcgcg
      961 gcctgttcgg ccagtgcggt gcacgtatgc gtccgggttc gacggcacgt cgtagttcac
     1021 gacgtgcgac acgtgctcga tgtccagtcc gcgggcagcc acgtccgtgg ccacgaggac
     1081 atccaggtcg ccgtcgcgaa acctgttcat gatccggtcg cgttgcgcct gatccatgcc
     1141 accatgaaga gccgcggcat cgtgcccacg cccgccgagc cgctccgcga gctcctccac
     1201 ctctccgcgc gtgcgtgcga agacgagcgc cgatgttggg tcttcgacat cgagtattcg
     1261 accgagcgcg gcgagcttat cggcgcgcct gaccacgtag gcaacctggc ggacgcgggc
     1321 gatgccgtcg ccgactctgt cctcggcgtg gacctcgacg cgctgcggat cccgcagatg
     1381 acgcgctgca atcctggcga tcggcgcgga gatcgtggct gagagcagcg ccgtctggcg
     1441 cgtgtcgggg gtcgctgaga gcagagtttc caagtcctcg gcgaagccca tgtcgagcat
     1501 ctcgtcggcc tcgtcgagga cgaccatctg gacgccttcg aggtggatgc ttctgcgctt
     1561 gacgtggtcg acggcgcggc cgggcgtggc cacgacgacg tcaaccccgc ggtccagggc
     1621 acgcagctgg aggccgattg cctgcccgcc atacacgggc agcacccgga tcgacagctt
     1681 ctcgccatag cgatggattg cctcggagac ctgcatcgcg agctcgcgcg tgggaaccag
     1741 aatcagcgcg gcgatgtggc cgtcggccga tttccccttg ccacggtcgt tcgccgcgat
     1801 cctctgtaga agcggcagcg cgaaggcggc tgtcttgccg gttcccgtgg gcgcctcggc
     1861 caggatgtcc tgtccttcca ggaggaacgg aatcgcctcg cgctggatcg gtgtcggttc
     1921 ctcgtaaccg agctcagcga gcgtctgcag gagccggtcg tcaaggccga gctgcccgaa
     1981 gccgtctacg gacttgggtg gggagccgcg cttggatttc gggatctcgt tggttttcgg
     2041 tttggtcgac tttgccacag gggctaatca tgacttgcgg gcagctccag gccgtacggg
     2101 ggacgggtca gcggcgtata ggagtcgtta aggtcaccag catgggcaag gtcatctacc
     2161 tcttgaacgt ttcgcttgac ggttacgtcg agacggcctc caagtcgctg gattgggcgc
     2221 tcgtcgacga ggagctccac gggtggttca acgatcagat gcgctcgact gacgcgtcgc
     2281 tctacgggcg acgactgtac gagttgatgt ccggccattg gccgcacgcg ggctcggacc
     2341 cgaactccac acccgtcgag aaggagttcg gtgagctctg gctggcgacg ccgaagatcg
     2401 tgttctcgtc gactctggag agcgtcgact ggaacagccg gctcgttcgc ggctcgcccg
     2461 ccgaagagct ggcgcgggtc cgcgaggagt tcccgggcga cctcgcggtt ggtggcgcca
     2521 cgctcgcgtc gtccttcatt cgcgccgggc tggtcgagga attccgcctc gtggtgcatc
     2581 ccgtcgtcat cggtggcggc acgccgttct tcccgccaac cgactcgccg atcccgcttc
     2641 ggctaaccga cactcacacc ttcagctctg gtgcgaccta ccgggcgtac tcgaagattg
     2701 actgatggag cgggcagacc gatcacgtgt gaaggcggtc gctaggctgg ggccgtggac
     2761 attgcccttg tcctgaacct gatcacgacc gtggcgatcg tcgtcgggct tgctttcgga
     2821 ttgtttgagg tccgccaagc gctgcgcgcc cggcgggacc tcgcggccgt ggacatcgtg
     2881 cggaccgtcc agactcagga aatccgtcgc gcaatgacac gcatcctctc ccttcccgat
     2941 gctgtcgctc cagaggtggt gcgtgctgat caggcgttgt tggacgcagc ggtcgcggtt
     3001 gacagtgcgt gcgaaatgtg gggctcgatg gtcttcgagg gcatcgtcga actgcacatg
     3061 ctggaccgca tggtcggcgg gtgggtacgt ggtacgtggg tccggctcga gccgtggatc
     3121 acacaggagc gaattgacaa gcgcacgccg agcgtcgccg aatggtggca gtggttatac
     3181 gagatgctcg aggccgaccc ggatccgggc aagaaacccg gagtgcatgt gtcctaccgc
     3241 gggaggatca ggaactagtc gcctcagccg agcttcgatc gatcttagtg cgcacgctgg
     3301 gcggctagcc tccgctgata cggtcctcgc aggccaaccg gccggcccat ggtctctggg
     3361 aggactttgt cgtgaagcca tttctggctg gcgcagctgc cttcgccctg atgggtgccg
     3421 tcttcgccgg ggtgtcgatg gccgctggac cggttacggc acaagcgccg aacgcgcgcc
     3481 tggcgctggt cgtggacggc ggcagcacta acggcagctt caccgtgctg cgcaagaagg
     3541 gcgtcacgtc tgtggcgaac ccctacgtcg gcgtgttctg cgtcacgccg agcgcgacgc
     3601 tggcattggg ccgcatcgtt cccatggttg gggtggacct gggcggatcg ccgttcgccg
     3661 actcaggagc ggcctggaac aggcagttgg tctactgccc gccggggacg atcgaagtcg
     3721 agacattcca ggtcagcacg ggcgctcacc gcaacgacgt tggcttcacg ctgacagtgt
     3781 tctagcgggc agcccgcgcc ggacacgcac gcgatcgctg ctcgtcaggc ccgcgcgcgg
     3841 ccggcatgtg cggcccgtct cgctgacatc ggggcgtgaa gctccgcgga cacatcgtcc
     3901 gcggccttga ggacttgctc tttcaccggg cccaatagtt gctctcgtcg gacagtcggt
     3961 gcagtcaccg ttaccgagcc gagcacgtcg ccgtgggcgt caaagaatgg agcagccacg
     4021 cccaggatcc cggcttcgaa catctggtcg tcaaaggcga aaccgttttc tctgattgcg
     4081 ggcagcttct caaagagctg gtcccggcgc gcggtcttct cctccgcaga caggcccaga
     4141 tgagcgtggt caagcacgag ttcgaggtcg ttctggccta gaaaggccat gaccacgcga
     4201 cccgacgccc ggcgatcctc ggaccctgag tagcccaccg caaggccagt gacacgcagc
     4261 gactgggcgc tctcgatcac gatctcgatg acgacccgat cagcgatcag gtgcgtcaag
     4321 tagaccgttt cgccgcactc ggccgcgaga cgctcgacga tcggccgcat ctgccgggcc
     4381 aggtcgctgc tgcgcgtcag cgccgcgtaa agcattccga cccgtccgcc gatgcgcaac
     4441 tcgccggcac tgtccttggc gaggtacccg cgcgcgacca gcgtgttgac gaggtgatac
     4501 gtcgaggaga cgttgtggcc caaagcatgt gcgagctttc gaaccggcat cggagcatca
     4561 gccgcgacga tctcgagcac gctcagcgcc cgatcgacgg tcttgagggt cgtcgcggtt
     4621 gcggctttgg cggtcatggt cctctcctgg ctatcggcgg acgtaatccc ttgacgggcg
     4681 ttcacatcaa ggtatagcgt tatttggtat tcctttcaaa tatccggaaa aggaacgatt
     4741 tgcatgaccg ccgacctcaa tgaccgctcc tggtggaacg agtaccggga cgagctgaac
     4801 cgcgacaagg aatggggaag cgccgcccgt ttctttcccg cgcggatcga gttcacccat
     4861 ggtgacggct cgtggaccat cgacacccgt gatggcgtgg cggtctccgt tacggagggc
     4921 gcccatccgc tcggctcgga tctggtcatt ggcgggccag atgaggagtg gcagcgcgtc
     4981 ctgaacggcg a
//
LOCUS       DATLCO010000027        11659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_771688, whole genome shotgun sequence.
ACCESSION   DATLCO010000027 DATLCO010000000
VERSION     DATLCO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771688"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VMZ33_00765"
     CDS             <1..606
                     /locus_tag="VMZ33_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HUR15790.1"
                     /translation="DAVDSYIPTPERAVDKPFLMPVEDVFGIKGRGTVATGRVERGIV
                     KVGEEVEIVGVKETRKVVVTGVEMFKKLLDEGRAGDNIGCLLRGIEREEIERGQVLAK
                     AGSVKPHTKFVARVYVLTKEEGGRHTPFYNGYRPQFYLRTTDVTGSIGLTGGAEMVMP
                     GDNVDMNVELITPIALETGQRFAIREGGHTVGAGAITEIVA"
     gene            696..863
                     /gene="rpmG"
                     /locus_tag="VMZ33_00770"
     CDS             696..863
                     /gene="rpmG"
                     /locus_tag="VMZ33_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HUR15791.1"
                     /translation="MAKAGENRPVIQLACTECKERTYSTTKNKKNDPNRIELRKYCPR
                     CRRHTLHREAK"
     gene            890..965
                     /locus_tag="VMZ33_00775"
     tRNA            890..965
                     /locus_tag="VMZ33_00775"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:923..925,aa:Trp,seq:cca)
     gene            1061..1246
                     /gene="secE"
                     /locus_tag="VMZ33_00780"
     CDS             1061..1246
                     /gene="secE"
                     /locus_tag="VMZ33_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HUR15792.1"
                     /translation="MSRIRRFIDESWSELKKVTWPTREQTRNLTVLVFVISAAVGAYI
                     AVFDVIFTMIVARIETL"
     gene            1273..1815
                     /gene="nusG"
                     /locus_tag="VMZ33_00785"
     CDS             1273..1815
                     /gene="nusG"
                     /locus_tag="VMZ33_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HUR15793.1"
                     /translation="MEKRWYVIHTYSGYEKKVKTNLEHRIESMGMEDKIFQVLVPEED
                     EIEIRGGQRVTVQKKVYPGYVLVEMILDPDSWFVVRNTPGVTSFVGGGNIPGSRNEPV
                     PLEEHEVKQILKQMGVETPKYRVAFQKGQSVRVTDGPFAEFIGTVDEVNPERNKLKVL
                     VSIFGRETPVELDFLQVEKL"
     gene            1830..2255
                     /gene="rplK"
                     /locus_tag="VMZ33_00790"
     CDS             1830..2255
                     /gene="rplK"
                     /locus_tag="VMZ33_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HUR15794.1"
                     /translation="MAKKIRAVLTLQLPAGAATPAPPVGTALGPHGINIVEFTKTYNE
                     KTQDKRGQIIPAQITIYEDRSFSFILKTPPAADLLRKAAGVEKGSQTTGREKVGKVTR
                     SQIREIAEIKMADLNAVDADGAAKIVEGTARSMGIEVVG"
     gene            2342..3049
                     /gene="rplA"
                     /locus_tag="VMZ33_00795"
     CDS             2342..3049
                     /gene="rplA"
                     /locus_tag="VMZ33_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HUR15795.1"
                     /translation="MPGRGKKYQQAAALVDREQTYQPTDAMELLKQTSYVAFDASAEA
                     HIRLGVDPRHADQMVRGTVVLPHGTGKRIRAVVFAQGEKAQEALRSGADEVGGEDLAK
                     RIEGGWLDFDVALATPDMMGVVGRLGRILGRRGLMPNPKSGTITFDLERAIKEVKAGR
                     VEFKVDKAGIIHTPFGKTSFESQQLSENLATLVDAINRARPSASKGQYFKGLTVAATM
                     GPGIRVDVPALLAAGAA"
     gene            3338..4120
                     /gene="rplJ"
                     /locus_tag="VMZ33_00800"
     CDS             3338..4120
                     /gene="rplJ"
                     /locus_tag="VMZ33_00800"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HUR15796.1"
                     /translation="MPTEAKQATVVELKEELSNSKAAIVADYRGLTVSDISAVRRTLR
                     ADGISYRVVKNRLAKIAATEVGRGELNEMLEGPTALALGKGDEVLLARTFLNAIRPYK
                     TVVIRGALLGDRRIDADSVTRLATLPPREVLLAQLAGGMISPVAMLASLFSAPLRNLG
                     YALTQLADMKGQEAGAAAVEAPNAEETSGEAPAAEARAEAPAETEESAEAPAEPAEAE
                     AAPAEVEASAEPATAESSAEAEPAADSEPAVDDNQSESESAQ"
     gene            4149..4547
                     /gene="rplL"
                     /locus_tag="VMZ33_00805"
     CDS             4149..4547
                     /gene="rplL"
                     /locus_tag="VMZ33_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HUR15797.1"
                     /translation="MATKVSQEDLLEAIDGMTVLELSDFIKKFEERYGVTAAAPAAAA
                     APAAAAAAGAAPAAAAEEQTEFSAILTDVGPNKIPVIKVVRELTGLGLKEAKDVVDAA
                     PKPIKEGVARDEAEKIKAALEEQGAKVEIK"
     gene            4688..5956
                     /locus_tag="VMZ33_00810"
     CDS             4688..5956
                     /locus_tag="VMZ33_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUR15798.1"
                     /translation="MTTTVRRIQAPTPRVALVLGAAIVLGILLYMARHALTPFIVGAL
                     LIYLLDPAVGWLSRQRIAGRRLPRGLAVLIVYVVVVVGFIYGLQLLLGPLVSQLVDYV
                     RDLPALLASVRETLDGLSRTYQSMDLPDEVRSFIDKALAGAGEGSSGVDFSALLPIAR
                     TIAGTAAGLFGFLIIPIWAFYILKDRVGLTQRFSQSLPPTWRDETWAILTIVERVFGR
                     WIRGQIILGLIVGAATFAGLLVLGWFVDPRFIQFAVFLAVIAGLLELLPIIGPIISMI
                     PTLLVALTTRDPATAVIGVLILYIAVQQLENAVLVPKIQGDAVELHPSLVIFVLIVGG
                     SIAGLLGAILAIPITAAARDVYRYLFRRLSDDEVPDPPATEDAEIDLLLAEAAAEETA
                     ADVAAEDEADADDADEPHSAKPGVPESPTP"
     gene            6309..9785
                     /locus_tag="VMZ33_00815"
     CDS             6309..9785
                     /locus_tag="VMZ33_00815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HUR15799.1"
                     /translation="MLSVTPDLGRRSPSSIERKRYARIPEVLPIPDLVELQRDSFRWF
                     IDNGLRELLDEISPIQDFTSRVMELRFREYEFEDPKYDELECRTRDLTYSKPLYVWVE
                     LLIKETGEIQRQRVYMGDFPFMTEEGTFIINGAERVVVSQLVRSPGVYYTAVEDPATG
                     RNLFNAKVIPNRGAWLEFETAARGQLYVKVDRKRKLEATKLLRAVGYESNDEIAALFT
                     RVDTAETAYIASTLDKDSTSTRGEALIEVYKKLRPGDPPTGDNAEKLVESLFFNFRRY
                     DLGRVGRYKLNKKLTQTADRMGITLNTDRTISKQDIASIVGHLIELNNGQGIADDIDH
                     LGNRRIRANGELIQNAFRVGLLRMERVVRERMSIQEAEKATPNALINIRPVVAAMKEF
                     FGGSQLSQFMDQTNPLAELTSKRRLSALGPGGLSRERAGFDVRDVHHSHYGRICPIET
                     PEGPNIGLIGSLATYGRINEYGFIETPYRKVKRTASYEDADVVNEIAEVDVATKSGTV
                     VLKRGAQFTKEAIAELKKQKAIAFPIKPRVTDEIVYLAADEEEEHHVAQANARLDDNG
                     YFIEDRIPARYRDAFPEARPNQIEYMDVSPKQVVSVATALIPFLEHDDANRALMGSNM
                     QRQAVPLLEPESPIVGTGMEERAALDSGQVLVAKRSGTVLSVTAERILIEPKEGAANE
                     LDEYPLEKFVRSNQGTCINQRPIVEVGQWIEGGQPIADSSSTDMGELALGRNILVAFM
                     SWEGGNYEDAIVLSDRLVKDDVFTSIHIEKHEIESRDTKLGPEEITRDIPNVGEESLK
                     DLDEDGVIYIGAEVQPGDILVGKITPKGETELTAEERLLRAIFGEKAREVKDSSLRLP
                     HGERGKIVEVTEFDREKGAELLPGVNRLIRVSVAAKRKISVGDKMAGRHGNKGVVAKI
                     LPQEDMPFLPDGTPVDIILNPLGVPSRMNIGQILETHLGWALKEQGNKTGTAVKAATA
                     VFDGANEDQIKDELRAAGLPESGKAVLHDGRTGEPFDREITVGYIYMLKLHHLVEDKI
                     HARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAANILQELLTVKSDDVMGR
                     VQTYEAIVKGEDIQAPGVPESFKVLIKELQSLGLNVEILNENEEEIHFAEDASAYPLP
                     DLGGINLAGFED"
     gene            9798..>11659
                     /locus_tag="VMZ33_00820"
     CDS             9798..>11659
                     /locus_tag="VMZ33_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HUR15800.1"
                     /translation="MLEVNNFNAIRISLASPEQIRDWSKGEVTKPETINYRTLRPEKD
                     GLFDERIFGPSRDWECYCGKYKRIRYKGIICDKCGVEVTRAKVRRERMGHIQLASPVS
                     HIWYFKGTPSRLGILLDISPRNLERILYFALYIVTWVDEDARQRLLQQLDDEAEGRGG
                     RAGKGLSELEDRLRADLNRDTDELNSSLATFKSEREEERTGRTAELVAAQQETKAKIE
                     ALGNGAAEEPIVFEPTGEILVAAGDKGGKTAQTALNKAAAAEIERVNTEIQLKESTQE
                     DDVRRQVEDRRLQLDATLESERERLKGEADGTKDEIRKARDEINRLQPGMMLPEASID
                     GFNFRDLDRKYGSGVRGGARLFAAGMGAEAVRELIQRMELDELGQKLHSEVRTTSGQR
                     RKKAIKRLRLIEAFRRSGNRPEWMILSVLPVIPPDLRPMVQLDGGRFATSDLNDLYRR
                     VINRNNRLKRLLELGAPEIIIRNEKRMLQEAVDALMDNGRRGRAISGTGNHKLKSLSD
                     MLRGKQGRFRQNLLGKRVDYSGRSVIVVGNDLKLHECGLPKKMALELFKPFVMRQLVE
                     RGLAHNIKSAKRYVERVSPEVWDVLEEVIKDHPVLLNRAPTLHRLGIQAFMPKL"
BASE COUNT         2280 a         3813 c         3735 g         1831 t
ORIGIN      
        1 gacgcggtcg actcgtacat cccgacgccc gagcgcgccg tcgacaagcc cttcctgatg
       61 cccgtcgagg acgtcttcgg catcaagggc cgcggcaccg tcgccacggg gcgtgtcgag
      121 cgcggcatcg tcaaggtcgg cgaggaggtc gagatcgtcg gcgtcaagga gacgcgcaag
      181 gtcgtggtca ccggcgtcga gatgttcaag aagctgctcg acgagggccg ggcgggcgac
      241 aacatcggct gcctgctcag aggcatcgag cgcgaggaga tcgagcgcgg ccaggtgctg
      301 gccaaggccg ggtcggtcaa gccgcacacc aagttcgtgg cccgcgtgta cgtcctgacc
      361 aaggaggagg gtggccgcca tacgcccttc tacaacggct accggccgca gttctacctg
      421 cgcaccaccg atgtcaccgg ctcgatcggc ctgaccggcg gcgcggagat ggtcatgccc
      481 ggcgacaacg tcgacatgaa cgtcgagctg atcacgccca tcgccctgga gaccggccag
      541 cgcttcgcca tccgcgaggg tggccacacc gtcggcgccg gcgcgatcac cgagatcgtg
      601 gcatgagcaa gatcgacacg ggcacccgct tgcgggaggg catggagagc gcgcagcagc
      661 ggcccgcagg gcgactgcgg aggacagagg tttagatggc caaggcagga gagaacaggc
      721 ccgtcatcca gctcgcctgc acggagtgca aggagcggac ctacagcacg accaagaaca
      781 agaagaacga cccgaatcgg atcgagcttc gcaagtactg cccacggtgc cgcaggcata
      841 ccctgcaccg tgaggcaaaa taggcgcgta aaggcttctc gtggctcgta ggcgcgtagc
      901 tcaattggta gagcactggt ctccaaaacc agcggttgca ggttcgagtc ctgtcgcgcc
      961 tgccaacact cgcaagctcg tgaggagcgc gcagcagcgg ccggcaggcc ggctgcggag
     1021 ccaaacagtc gtaatcaacc ctcagaggta agtcaatcca gtgtcccgaa tccgccggtt
     1081 catcgacgaa tcgtggtcag aactgaagaa ggtcacgtgg cctacgcgcg agcagacgcg
     1141 caatctgacc gtgctggtct tcgtcatcag cgcggcggtc ggcgcctata tcgcggtgtt
     1201 tgacgtgatc ttcaccatga tcgtggctcg gatcgagacg ctgtgatgga agccgaagcg
     1261 cctgtcaagg cgctggagaa gcgctggtac gtgatccaca cgtattcggg ctacgagaag
     1321 aaggtcaaga ccaacctcga gcatcggatc gagtcgatgg gcatggaaga caagatcttc
     1381 caggtcctgg taccggaaga ggatgagatc gagatccgcg gcggccagcg cgtcaccgtg
     1441 cagaagaagg tctacccggg ctacgttctc gtcgagatga tcctcgaccc cgactcgtgg
     1501 ttcgtcgtcc gcaacacgcc gggcgttacg agcttcgttg gtggcggcaa catccccggc
     1561 tcacgcaacg agccggtgcc gctcgaagag cacgaggtca agcagatcct caagcagatg
     1621 ggcgtcgaga cgcccaagta ccgcgtcgct ttccagaagg gccaaagcgt gcgcgtgacc
     1681 gacgggccgt tcgccgaatt catcggcacg gtcgacgagg tcaaccccga gcgcaacaag
     1741 ctcaaggtcc tggtcagcat cttcgggcgc gagacgcccg tggagcttga cttcctccag
     1801 gtagagaaac tctaggagcc gcccgcacca tggctaagaa gatccgagct gtgctcacac
     1861 tgcagctgcc ggccggtgcg gccacgccgg cgccgccggt cggcaccgcg ctcggcccgc
     1921 acggcatcaa catcgttgag ttcaccaaga cctacaacga gaagacccag gacaagcgcg
     1981 gccaaatcat ccccgcgcag atcacgatct atgaggatcg aagcttcagc ttcatcctga
     2041 agacgcctcc cgccgcggac ctgctgcgca aggccgccgg cgtcgagaaa ggctcgcaga
     2101 ccaccggccg cgagaaggtc ggcaaggtga cgcgcagtca gatccgtgag atcgccgaga
     2161 tcaagatggc cgacctcaac gccgtcgacg ctgacggtgc ggccaagatt gtcgagggca
     2221 ccgcgcgctc gatgggcatc gaggtcgtcg gctagcaatc cagtcgtcgc gcgggcaatc
     2281 cgcgcgcgta caccccgcgt gagagggaag gcccatgtgg ccgtcagaag ggagagctca
     2341 gatgcctggt agaggcaaga agtatcagca ggccgcggcc ctcgttgacc gcgaacagac
     2401 ctatcagccg acggacgcga tggagctgct caagcagacg tcctacgtcg ctttcgatgc
     2461 ctccgccgag gcccacatcc ggctaggtgt cgacccgcgc cacgccgacc agatggtccg
     2521 cggcacggtc gtgctgccac acggcacggg taagcgcatt cgcgcagtcg tattcgccca
     2581 gggtgagaag gcccaggaag cgctgcgctc cggcgccgac gaagttggcg gggaagacct
     2641 ggccaagcgc atcgagggtg gctggctcga ctttgacgtc gccctggcga cgcccgacat
     2701 gatgggcgtg gtgggccggc tcggccggat cctcggccgt cgggggctca tgcctaaccc
     2761 gaagtcgggc accatcacgt ttgatctcga acgtgcgatc aaggaagtca aagccggccg
     2821 tgtcgaattc aaggtcgaca aggcaggcat cattcacaca cccttcggaa agacgagctt
     2881 tgagtctcag cagctgagcg agaacctcgc cacgcttgtc gacgcgatca accgcgctcg
     2941 gccgagcgct tccaagggcc agtatttcaa gggtttgaca gtggcggcca cgatgggccc
     3001 gggcatccgg gtcgacgtgc cggcgcttct cgccgcgggc gccgcgtaag gcactcgctc
     3061 gttcggggta agatctcgac ttcactcaac atcgttccgc cgcagacagc gctgcgctcc
     3121 cagggctcga ttctcctaaa ggggtaacgg cctgacggac cgaaatcgtc gaccagtccc
     3181 aattcgggac catgccgacg ggcacgccga ggcagggatg acgtctcggg ttcacggctc
     3241 cggctgagaa cccacgttgc tgacgcagcg tcgacgccct cgcgcggaac caagcgcaag
     3301 gacgtccggc gcgccggacg tggggaaagg aggccaaatg ccgacggagg ccaagcaggc
     3361 cacggtcgtc gagctgaagg aggagctctc caacagcaag gctgccatcg tggccgacta
     3421 tcgcggcctg accgtttcgg acatcagtgc tgtccgtcgc acgttgcgtg ccgacggcat
     3481 cagctatcgc gttgtcaaga atcgcctggc caaaatcgct gcgaccgaag tcggacgtgg
     3541 cgagctgaac gagatgctcg agggtccgac ggccctggca ctcggcaagg gcgacgaggt
     3601 cttgctcgcg aggacgttcc tgaacgcgat ccgtccgtac aagacggtcg ttattcgtgg
     3661 cgcgctcctc ggcgatcgac gcatcgacgc cgacagcgtt acccggctgg caaccctgcc
     3721 gccgcgcgag gtgctcctcg ctcaactggc aggcggcatg atctcgcccg tggcgatgct
     3781 cgcgtcgttg ttctcagcgc ccctccgtaa cctgggctac gcactgactc aactcgccga
     3841 catgaagggc caggaggcgg gcgcagcggc agtcgaggcg cccaacgccg aggaaacctc
     3901 aggggaggct ccagcagccg aggcacgcgc agaggctcca gccgagaccg aagagtcagc
     3961 cgaagcgccc gcagaacctg cggaggccga agcggcaccc gcggaggtcg aagcatcagc
     4021 cgagcctgcg acagccgaaa gctccgcaga agcggagccc gctgccgact cagagcccgc
     4081 tgtcgatgac aaccaatccg aatctgaatc cgctcaataa ccaagacggc aaaggagaac
     4141 tgctaacaat ggctacgaag gtgtcccagg aggatcttct cgaggcgatc gacggaatga
     4201 ccgtccttga gctgtccgat ttcatcaaga agttcgagga gcgctacggc gttacagccg
     4261 cagctccggc cgcagctgca gctccggccg ccgctgctgc cgctggcgct gccccggccg
     4321 ccgctgcgga ggagcagacc gagttcagcg ccatcctgac tgacgtcggc ccgaacaaga
     4381 tccccgttat caaggtcgtt cgcgagttga ccggactggg cctcaaggag gccaaggacg
     4441 tagttgatgc tgcgccgaag ccgatcaagg aaggcgttgc gcgcgacgaa gccgaaaaga
     4501 tcaaggctgc tctcgaggag caaggcgcga aggtcgagat caagtaactc gaccggccgg
     4561 acgttcgacc ggcccacatc gcagtcaccc aagggctcgc cgaaaggcga gctcttccct
     4621 ttacagcccg ggggtcggcg agccggttca atgctcctga acgccgacag accgctataa
     4681 agggggtgtg acgaccaccg tgcggcgaat ccaggcgccc acgcctcgag tggcactcgt
     4741 actgggcgcg gcaatcgtgc tcggaatcct tttgtacatg gcgcggcacg cgctcacgcc
     4801 gttcattgtc ggtgcgctgc tcatctacct cctcgacccg gccgtaggct ggctgagtcg
     4861 ccaacgcatc gccggccgca ggctgccgcg cggactcgcc gtcctgatcg tgtacgtcgt
     4921 ggtcgtggtc ggcttcatct acggcttgca actgttgctg ggcccgttgg tgagccagct
     4981 ggtcgactac gttcgtgacc taccggcgct gctggccagc gtgcgcgaaa cgctggatgg
     5041 actgagtcgg acgtaccagt cgatggacct gccggacgag gttcggagct tcatcgacaa
     5101 ggcgctggct ggtgcgggtg aaggctcgag cggcgtcgat ttcagcgccc tcttgcccat
     5161 tgcccggacg atcgcgggca cggctgccgg attgttcggc ttcctcatca tcccgatctg
     5221 ggcgttctac atcctgaagg accgagtcgg gctgactcag cgtttcagtc agtcgctgcc
     5281 accgacgtgg cgtgacgaga cctgggcgat cctgacgatc gtcgagcgag tcttcggtcg
     5341 ctggatccgc ggtcagatca tcctgggcct gatcgtcggc gccgcgacct tcgccggact
     5401 cctcgtgctc ggctggttcg ttgatccgcg gttcatccag tttgccgtct tcctggcagt
     5461 catcgccggc cttctcgagc tcctgccgat aatcgggccg atcatctcga tgatccccac
     5521 gcttcttgtg gcgctgacaa cccgcgaccc ggccacggcg gtcatcggcg tgctgatcct
     5581 ctacatcgcg gtgcagcagc tcgagaacgc cgtgctcgtg cccaagattc agggcgacgc
     5641 ggtcgagctg cacccgtccc tggtgatctt cgtgctgatc gtgggcgggt cgattgccgg
     5701 actgcttggg gcaattctcg ccatcccgat aacagccgcg gcacgcgacg tgtaccggta
     5761 tctgttccgg cgattgagcg acgacgaggt gcccgaccct ccagcgactg aggacgccga
     5821 aatagaccta ctgctcgccg aagcggccgc cgaagagaca gcggctgatg tagccgccga
     5881 ggacgaggcc gacgcggacg acgcggacga gccgcattcg gccaagccgg gtgtccccga
     5941 gagcccgacg ccctagacgt tcgctttcct ctctcactga gacgaagccg gccggcgggt
     6001 ccgggagcag atcactccgc ccgtactact tgcagaaacg acccggttcg tggtcaaatg
     6061 cgcgccgcgg acgcagctcc gatacttccg tgtcgggcga gcaggccgtt tgacagcctg
     6121 ccaatcgctc gatactatgt gcggtcgttg agcgcggctc ccttttcgtc ggaaccttca
     6181 tggcggacgc ccctgtccgc ctttctgcgt ctccgaaatg tctgccgtgg tcgtgctgtc
     6241 attcgccgct ctttcgccac tctgtcgtct gtccaaacgc cgccccgtta ccgccgctag
     6301 gagccttgat gctgtctgtc acccctgatc tcggccgccg tagcccctcg agcatcgagc
     6361 gcaagcgcta cgcacggatc cccgaggtcc tgccgatccc cgatctcgtc gagcttcagc
     6421 gcgactcgtt ccgatggttc atcgacaacg gtctgcgcga gctgttggac gagatcagcc
     6481 cgatccagga cttcaccagc cgggtcatgg agctgcgctt ccgcgagtac gagttcgagg
     6541 atccgaagta cgacgagctt gagtgccgca cgcgcgacct cacgtactcc aagccgctct
     6601 acgtatgggt cgagctcctg atcaaggaga ccggtgagat ccagcgtcag cgcgtgtata
     6661 tgggcgactt cccgttcatg acggaggagg ggacgttcat catcaacggc gcggagcgcg
     6721 tcgtcgtgag ccagctcgtc cgctcgccgg gcgtctacta cacggctgtg gaggacccgg
     6781 cgaccggccg caacctcttc aacgcgaagg tcatcccgaa ccgcggcgcg tggctggagt
     6841 tcgaaaccgc ggcccgtgga cagctgtacg taaaggttga ccgcaagcgc aagctcgagg
     6901 ccaccaagct cctgcgcgct gtcggctacg agtcgaatga cgagattgcg gcgctgttca
     6961 cgcgcgtgga cacggctgag accgcctaca tcgccagcac cctcgacaag gacagcacga
     7021 gcacacgtgg tgaggccctc atcgaggtct acaagaagct tcgccccggc gacccgccca
     7081 cgggcgacaa cgccgagaag ctagtcgaga gcctcttctt caacttccgg cgctacgacc
     7141 tgggccgtgt gggccgctac aagctgaaca agaagctgac ccagacagcc gatcgcatgg
     7201 gcatcacgct caacaccgac cggaccatca gcaagcagga catcgcgtcc atcgtcggcc
     7261 acctgatcga gctcaacaat ggccagggca tcgccgacga catcgaccac ctcggcaacc
     7321 gtcgcattcg cgccaacggg gagctgatcc agaacgcctt ccgggtcggc ctcctgcgca
     7381 tggagcgcgt cgtgcgcgag cgcatgtcga tccaggaggc tgagaaggcc acgcccaacg
     7441 ccctcatcaa catccgcccc gtcgtggcgg cgatgaagga gttcttcggc ggcagccagc
     7501 tcagccagtt catggatcag acaaaccctc ttgccgagct cacgtcgaag cgccgcctct
     7561 ccgctcttgg ccccggtggc ctgagccgcg agcgcgccgg gttcgacgtt cgcgacgtgc
     7621 atcacagcca ctacggacgc atctgcccga ttgagacccc ggaaggtccg aacatcggcc
     7681 tcatcggctc gctcgcgacg tatggccgca tcaacgagta cggcttcatc gagacgccct
     7741 accgcaaggt gaagcgaacc gccagctacg aggatgcgga cgtcgtcaac gagatcgccg
     7801 aggtcgacgt tgcaacgaag tccggcacgg tcgtcctgaa gcgtggcgct cagttcacca
     7861 aggaggcgat cgccgagctg aagaagcaga aggcgatcgc gttcccgatc aagccgcgcg
     7921 tcaccgacga gattgtgtat ctcgcggccg acgaggaaga ggagcaccac gtcgcccagg
     7981 ccaacgcgcg cctggatgac aacggctatt ttatcgagga ccgcatcccg gcgcgttacc
     8041 gcgacgcgtt ccccgaggcg cgccccaacc agatcgagta catggacgtc agccccaagc
     8101 aggtcgtctc cgtggcgact gccttgatcc cgttcctcga gcacgacgac gccaaccgcg
     8161 cgctcatggg ttcgaacatg cagcgccagg cagtgccgct gctcgagccc gagtcgccga
     8221 tcgtcggcac cggcatggag gagcgcgctg cgctcgactc cggccaggtc ctcgtggcca
     8281 agcgttcggg caccgtgctc agcgttaccg ccgagcgcat cctgatcgag cccaaggagg
     8341 gcgcggcaaa cgagctcgac gagtatccgc tcgagaagtt cgtgcgttcc aaccagggca
     8401 cgtgcatcaa tcagcggccg atcgtcgagg tcggacagtg gatcgagggc gggcagccca
     8461 tcgcggactc cagctcgacc gacatgggcg agctcgcgct cgggcgcaac atcctggtcg
     8521 cgttcatgag ctgggagggc ggcaactacg aggacgcaat cgtcctttcg gaccgcctcg
     8581 tcaaggacga cgtgttcacg tccatccaca ttgagaagca cgagatcgag tcacgcgaca
     8641 cgaagctcgg ccctgaggag atcacgcgcg acattccgaa cgtcggcgag gagagcctca
     8701 aggacctcga cgaagatggc gtgatctaca tcggcgctga ggtccagccg ggcgacatcc
     8761 tcgtgggcaa gatcacaccc aagggcgaga ccgagctgac ggcggaggag cgcctgctcc
     8821 gcgcaatctt cggtgagaag gcgcgcgagg ttaaggactc cagcctgcgg ttgccgcacg
     8881 gcgagcgtgg caagatcgtc gaggtcactg agttcgaccg cgagaagggc gccgagctcc
     8941 tgcccggcgt caatcgcctg atccgcgtca gcgtcgccgc gaagcgcaag atcagcgtcg
     9001 gggacaagat ggccggccgc cacggaaaca agggtgtcgt ggccaagatc ctgccccagg
     9061 aggacatgcc attcctcccc gacgggacgc cggttgacat catcctcaac ccgttgggcg
     9121 tgccttcccg aatgaacatc ggccagatcc tcgagaccca cctcgggtgg gcgctcaagg
     9181 agcagggcaa caagaccggc accgccgtca aggcggccac ggccgtgttc gacggtgcca
     9241 acgaagatca gatcaaggat gagctgcgcg ccgcgggact gcccgagtcc ggcaaggccg
     9301 tcctccatga cggccgcacc ggcgagccgt tcgatcgcga gatcaccgtc ggctacatct
     9361 acatgctcaa gctgcaccac ctggtggaag acaagatcca cgcccggtcg accggcccgt
     9421 acagcctgat cacgcagcag ccgctgggtg gcaaggcgca gttcggtggt cagcgcttcg
     9481 gggagatgga ggtgtgggcg ctcgaggcct acggggcagc gaacatcctc caggagctgc
     9541 tgacggtcaa gtccgacgac gtcatgggcc gtgtccagac gtacgaagcg atcgtgaagg
     9601 gtgaggacat ccaagcgccg ggcgtacccg agtcgttcaa ggtcctcatc aaggagctcc
     9661 agagcctcgg cctcaacgtc gagatcctca acgagaacga ggaagaaatc cacttcgccg
     9721 aggacgcctc ggcctatccg ctaccagacc ttggcgggat caacctcgcc ggtttcgaag
     9781 actaggagta cccagtcttg ctcgaagtca acaatttcaa cgcgatccgg atctctcttg
     9841 cgtcacctga gcagatccgg gactggtcga agggcgaggt aacaaagccc gagaccatca
     9901 actaccgcac gctgcgccct gagaaggacg gccttttcga cgagcgcatc ttcggtccca
     9961 gccgcgactg ggagtgctac tgcggcaagt acaagcgcat ccgctacaag ggcatcatct
    10021 gtgacaagtg cggcgtcgag gtcactcgcg ccaaggttcg tcgcgagcgg atgggccaca
    10081 tccagctggc cagcccggtc agccacatct ggtatttcaa gggcacgccg agtcgcctcg
    10141 gcatcctgct cgacatcagc ccgcgcaacc tggagcggat cctctacttc gcgctgtaca
    10201 tcgtcacgtg ggtcgacgag gacgcgcgac agcggctact ccagcagctc gacgatgagg
    10261 ctgagggtcg tggcggtcgc gccggcaagg gtctgtccga gctcgaggat cggcttcgcg
    10321 ccgacctgaa ccgcgatacg gacgagctca actcgagcct cgccacgttc aagtcagagc
    10381 gcgaggaaga gcgcaccggc cgcacggccg aactcgtcgc cgcgcagcag gagaccaagg
    10441 ccaagatcga ggcgctcggt aacggcgctg ccgaggagcc catcgtcttc gagccgaccg
    10501 gcgaaatcct ggtggcggct ggggataagg gcggcaagac cgcccagact gcgttgaaca
    10561 aggccgccgc ggctgagatc gagcgcgtca acaccgagat ccaactcaag gaaagcaccc
    10621 aggaagacga cgttcgtcgc caggtggagg acaggcgcct ccagttggac gccacgctcg
    10681 agtcagagcg tgagcggctc aagggtgagg ccgacggcac caaggacgag atccgcaagg
    10741 cgcgcgacga gatcaatcgt ttgcagccgg gcatgatgct gcccgaggcg agcatcgacg
    10801 gcttcaactt ccgcgacctc gatcgcaagt acggctcggg tgtccgtggt ggcgctcggc
    10861 tgttcgcagc cggaatgggc gctgaggccg ttcgcgagct gatccagcgc atggagctcg
    10921 acgagctcgg ccagaagctg cacagcgagg tccgcacgac cagcggtcag cgccggaaga
    10981 aggcaatcaa gcggctgcgc ctcatcgagg ccttccgtcg ttcggggaac cgtcccgagt
    11041 ggatgatcct gtccgtcctg cccgtaatcc cgcccgacct gcggccgatg gtccagctcg
    11101 acggtggccg cttcgccacg agcgacctca acgacctgta ccgccgcgtc atcaaccgga
    11161 acaaccggct gaagcggctg ctcgagctcg gcgccccgga gatcatcatc cggaacgaga
    11221 agcggatgct ccaggaggcg gtcgacgccc tcatggacaa cggccgccgc ggccgcgcca
    11281 tctcgggcac cggcaaccac aagctgaaga gcctctccga catgctccgc ggcaagcagg
    11341 gccggttccg tcagaacctg ctcggcaagc gcgtggacta ctccggccgc tcggtcatcg
    11401 tggtcggcaa cgacctgaag ctccacgagt gcggcctgcc caagaagatg gcgctcgagc
    11461 tgttcaagcc gttcgtcatg cgccagctcg tcgagcgcgg cctcgcgcac aacatcaaga
    11521 gcgccaagcg ctacgtcgag cgcgtgtcgc ccgaggtgtg ggacgtcctg gaagaggtca
    11581 tcaaggacca cccggtcctc ctcaaccgcg cacccaccct gcaccgcctc ggcatccagg
    11641 cgttcatgcc caagctcat
//
LOCUS       DATLCO010000028         4050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8966938, whole genome shotgun sequence.
ACCESSION   DATLCO010000028 DATLCO010000000
VERSION     DATLCO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8966938"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..958
                     /locus_tag="VMZ33_00825"
     CDS             197..958
                     /locus_tag="VMZ33_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HUR15801.1"
                     /translation="MTVAARLERTYRVRFDEAGPDGFLRASGFVRYAQDMAWIHSESA
                     GFGRTWYRDRGLFWLVRGVELDLLEDVDYGTELVVSTEVIGFRRVIARRRSEFHLPES
                     ERPVATVLTDWVLLKDNGRPVQPPAEILGMFPPALHEFAPLRARVGEPPAHADEAAFS
                     VRRSEADPMGHVNNAVYVDYLDEGFLRRAARDGGLPVPRRYRVEFAGSALPGSRVTAR
                     GWDEPLSWSCTLTDEDGHETLRATLETDSAAWVGG"
     gene            complement(935..1672)
                     /locus_tag="VMZ33_00830"
     CDS             complement(935..1672)
                     /locus_tag="VMZ33_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HUR15802.1"
                     /translation="MASARTTGVERMLVPGWDLESSRMSVELARRAGVGASAGIHPHV
                     ASEQGESAWHAVAAMVSLPHVVAVGETGLDYDRGFSPRDAQLANLRWHLGLARDSGKP
                     AILHCRSKPGERDAQDDLIRELAEAGGPPALLHSFSGPVDYAERALEMGLYVSFSGLA
                     FRDGEEVTADVACLVPADRLLVETDSPYLSPPGAPRRRNTPEWVRVTAAWLAQQRGTT
                     LDELGPQLVSNYDRLFTKSESAPDPRG"
     gene            complement(1725..>4050)
                     /locus_tag="VMZ33_00835"
     CDS             complement(1725..>4050)
                     /locus_tag="VMZ33_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR15803.1"
                     /translation="PAIIVLEDLHWADESLLDFIESLARWADGPILLLCLARHELLER
                     RPRWAGGIRNSTTLVLEPLDSAANEQLIDGLTAGGMPKLLRDRIVELADGNPLFSEEL
                     IRMFIDRGVLRSVDGRWELASTVDSVEVPGTVQAVLAARLDALPIAEKVVAQDASVVG
                     RVFWDAVVASLEQAPVSAVDDLLRRLRVKELVVPREPSRFSGADEFSFRHALIRDVAY
                     EGLPKRDRADKHLAVIEWAERELDERPGEAAELVAAHYLAALRYREEFAGDASELASL
                     RKRAYASVVLARDRAGSLHEKAGATRLARIALELARALDLDVMERTRAAVAFLDLGSG
                     HWLRDEGVVVARQAIDDLEHYDEYSAERDRLEALVRSSLAWILLGQGGADEARAGLLS
                     QLARLESGPPTDTRAWLLARMGWLSWRVGPAESGLPYLERALVEARASGSRLTEAWAL
                     HDLGVVAGQMGDSDKAARLIRQSLEVARESGDHALIVRCFVNLPAMTMDNGPDWDELY
                     EWLEDGLALVRRASDREAEAWMAWQLSDYWEFRGDFVLAIEYCRLSVELAEQHGDMAS
                     GAARRVGLAWLEAHLGRWPAMNTDGVDDAAVAPGENQYLAWGTAWRAVRAWRDRPAAG
                     LALIRGGLGGMSTEARAPALWGARMAYRLGDEDVIRMGQSNTGGGRTVAALVDAWMAA
                     FLDDVASAPHGLARVVAEADRLGYRNFAQMARDDLALAVARSGRDASAEVRNAVEFAR
                     ALGIQPLLGPLPETRWLTAGPDDGGKVAVDAAAD"
BASE COUNT          688 a         1449 c         1281 g          632 t
ORIGIN      
        1 gaccgatgcc ggcgcccagg ctccagttcg gcccgacgtc aacgtgtcgc agccgctcca
       61 gatgccgcac gcgcgacccg atttcgaaaa tcggaaggac gccgcggtcg acgacatgct
      121 ggccgcgcct gcggtgcaag ccgagccatc ggacgtcgat actcgctgat caaccgacag
      181 ccatagactc gcgccgatga ccgtcgccgc gagactcgaa cgaacctata gggtccgctt
      241 tgacgaggcg ggccctgacg gttttctgcg cgcctctggc tttgtccgtt acgcccagga
      301 catggcctgg atccactctg agtccgctgg cttcggacga acctggtacc gcgaccgagg
      361 cctgttctgg ctagttcgtg gtgtggagct ggaccttctg gaagacgttg actacggcac
      421 ggagctagtc gtttcgaccg aggtcatcgg ctttcggcgt gtcatcgcgc ggcgcaggag
      481 tgagttccac ctgcccgaat ccgaacgtcc ggtggcgacc gtcctgaccg actgggtgtt
      541 gctcaaggac aacggccgtc ccgtgcaacc cccggctgag atccttggaa tgtttccgcc
      601 ggctttgcac gagttcgcgc cactccgggc gcgcgtcggc gaaccgccgg ctcatgcgga
      661 tgaggcggca ttctctgtcc gtcggagcga ggctgacccc atgggccacg tgaacaacgc
      721 cgtgtacgtc gactatctcg acgagggctt cctgcgccgg gcagcgcgcg acggcgggct
      781 gccggtgccg cgccgctatc gcgtcgaatt cgccggctcg gcgctgcccg gctcgcgggt
      841 cacggcacgc ggttgggacg agccactgtc gtggtcatgc acgctcaccg acgaggacgg
      901 ccacgaaacc ctccgagcga ccctggagac ggattcagcc gcgtgggtcg ggggctgact
      961 cggacttcgt gaacaggcgg tcgtagttcg agactagctg gggtccgagc tcgtcaaggg
     1021 tcgtgccgcg ctgctgcgcc agccaggcgg ccgtgacacg tacccactcc ggcgtgttac
     1081 ggcggcgcgg cgctcccggc ggtgacagat agggcgaatc ggtttccacg agcagtcggt
     1141 ctgcgggcac caggcaagcc acgtcagcgg tcacctcctc cccatcccgg aacgccagcc
     1201 cgctgaaact gacgtacagc cccatctcga gcgcgcgctc ggcgtagtcg accggacctg
     1261 agaacgagtg aagcagcgct gggggcccgc cggcctccgc cagctcgcgg atcaggtcgt
     1321 cctgggcgtc cctttccccg ggcttggaac ggcagtggag aatcgccggc ttgccgctat
     1381 cgcgcgcgag ccccaggtgc cagcggaggt tggcgagctg tgcatcgcgc ggcgagaagc
     1441 cacggtcgta atcgagccct gtctcgccga ccgccacaac atggggcagg gacaccatcg
     1501 ctgcgaccgc gtgccaagcc gactcgcctt gctcgctggc cacgtgcggg tggatacccg
     1561 ccgaagcgcc aacacccgct ctccgcgcca attccactga catgcgcgac gactccaggt
     1621 cccaccctgg cacgagcatg cgttcgacgc ccgtggtccg cgcactggcc aacacctgct
     1681 cggcgtcgga atcgaaggcc gtggcctgaa ggtgcgcatg ggaatcaatc agccgcggcg
     1741 tcgacagcga cctttcctcc gtcgtcgggg cccgcggtca gccatcgcgt ttccgggagc
     1801 ggtccgagca gcggctgaat gccaagcgca cgtgcgaact cgaccgcgtt gcgaacctcc
     1861 gcggacgcgt cacgtccgct tcgcgcgacc gccagcgcaa gatcgtcgcg cgccatttga
     1921 gcaaagttgc gatatccgag acggtcggcc tcggcgacca cgcgggcgag tccatgcggc
     1981 gcactggcca cgtcatcgag gaaagccgcc atccacgcgt ccaccagcgc ggccacggtg
     2041 cgaccgccac ccgtgttcga ttggcccatc cggatcacgt cctcgtcgcc cagccgatag
     2101 gccatgcggg caccccatag ggcgggcgct cgtgcctcgg tggacatccc gcccagtcct
     2161 cccctgatca gtgcgagccc cgccgcggga cggtcccgcc acgcccgcac cgcacgccac
     2221 gctgttcccc aggccaagta ctggttctct cccggagcaa ccgctgcgtc atccactcca
     2281 tccgtattca tggctggcca ccgaccgagg tgtgcctcga gccacgccag cccaacacgt
     2341 ctggccgcgc cgctcgccat gtccccgtgt tgttcggcca gttcgacgga caggcggcaa
     2401 tactcgatcg ccagcacaaa gtcaccacga aactcccagt aatccgagag ctgccaggcc
     2461 atccacgcct ccgcctcgcg gtcggaggca cgccggacga gcgccaagcc atcctccagc
     2521 cactcgtaga gctcgtccca gtccggcccg ttgtccatgg tcattgccgg caggtttacg
     2581 aagcagcgga cgataagcgc gtggtcgcct gactcacgcg ccacctccag gctttggcgg
     2641 atcaatcgcg ccgccttgtc gctgtcgccc atctgtcccg cgacgacgcc caggtcatgg
     2701 agcgcccacg cttcggtcag tcgcgatccg ctcgctcgag cctcaaccag ggctcgctcg
     2761 agatatggca agccactctc cgccgggccg acacgccacg acaaccagcc catccgcgca
     2821 agcagccacg cccgggtgtc agtcggcgga cccgactcca gccgtgcgag ctggcttagc
     2881 agcccggcac gggcttcgtc ggcgccacct tgcccgagca ggatccaggc aaggctgctc
     2941 cgcacgaggg cctcgagtcg atcgcgttcg gctgagtact cgtcatagtg ctcgaggtcg
     3001 tcgatcgcct gacgggcaac gacaactccc tcgtcgcgca gccaatgtcc cgacccgaga
     3061 tcgaggaatg cgacggcggc cctcgtccgc tccatcacat ccagatcgag cgctctggcg
     3121 agctccagcg cgattcgcgc caagcgagta gcccccgcct tttcgtgcag tgatccggcg
     3181 cgatcgcgcg caaggaccac agaagcgtaa gcacgcttcc ggaggctggc cagctctgag
     3241 gcatcccctg cgaactcctc gcggtaacgg agcgcggcca ggtagtgcgc agcaacaagc
     3301 tcggcggcct caccgggtcg ttcgtcgagc tcgcgctcgg cccattcgat cacggctagg
     3361 tgcttgtcgg cccggtcgcg cttggggagt ccctcatagg ccacgtcgcg gatcagcgca
     3421 tgccggaagc tgaactcgtc ggccccggag aagcgcgacg gttcccgtgg aaccaccagc
     3481 tccttgacgc ggagccgcct gagcaaatcg tcgacggccg acacgggcgc ctgctccagc
     3541 gaggcgacga cggcatccca gaagacgcgg ccgacaacgg acgcatcctg ggcgacgacc
     3601 ttctccgcga tgggaagagc gtcaagacgc gccgcaagga cggcctggac ggtccccggc
     3661 acctccacag aatcgaccgt tgaagcgagc tcccagcggc catccaccga ccgcaacacg
     3721 ccacgatcaa tgaacatgcg gatcagctct tcggagaaca gcgggtttcc gtccgcgagc
     3781 tccacgatcc gatcgcggag cagcttcggc atgcccccgg ccgtgaggcc gtctatcagc
     3841 tgctcgttcg ctgccgaatc aagtggctcc agcacgagcg ttgtcgagtt gcgtatgccg
     3901 ccggcccaac gcggacgacg ctccagaagc tcatggcgag cgaggcacag cagcaggatc
     3961 ggaccgtcgg cccagcgggc cagagattcg atgaaatcga gcaggctctc gtcagcccag
     4021 tggaggtcct ccagcacgat tatggctggc
//
LOCUS       DATLCO010000029         3844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8211333, whole genome shotgun sequence.
ACCESSION   DATLCO010000029 DATLCO010000000
VERSION     DATLCO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8211333"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VMZ33_00840"
     CDS             complement(<1..807)
                     /locus_tag="VMZ33_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HUR15804.1"
                     /translation="MATTPTSAPTATSTLEPTLEPTLEPTLEPTPALTAAPSVQPSME
                     PTGTPEPVETPSQATGDALFFDEMDDPTSGWSTGSADFAEIGFDVTSLQIELLQAPAY
                     AYSQRALDEEYGVVLSAAEFTPSTEGGAALLCLGPSDAGGDVGVGALFTTEGDLLFID
                     VSNNDITTLERNDAVGLDMAIGGTSLFGVACAGTSTGALRIVAFGDHTGPLATYQSDE
                     GPETFSGVGVYGESSTVGFTLDVGTAATYGISGSALGMSPEGEELLTHVPS"
     gene            complement(1096..2940)
                     /gene="thrS"
                     /locus_tag="VMZ33_00845"
     CDS             complement(1096..2940)
                     /gene="thrS"
                     /locus_tag="VMZ33_00845"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HUR15805.1"
                     /translation="MAIDEQTIDQQEDELELEAPERTSAEELLDASAGSPLDPMRHSA
                     AHVMAEAVMDLFPGTKLGIGPPIQDGFYYDFDLPRPLTSDDLAAIEARMNESIAADHP
                     FVFSERSNADARAFLLEKEQPYKVEIVDDLVAGAASRGLPPPKTTFYEQGPFIDLCRG
                     PHVESTGKIGPIKLLAVSGSYWRGDQKRPQMQRVYGTAWPTQEELDAFLWRREEAKKR
                     DHRKLGVQLDLFSFHDVSPGGAFWHPKGWRLFNTLRDAMRDIQRRRGYQEIYTPPLVH
                     QRLWEQSGHWDLYRDKMFLVESDDQTYSLKPMNCPESTFIYRSKVRSYREFPLRLSEY
                     GALHRNELSGVTSGLTRVRHFYTDDAHIYVTPAQIQDEILALMGEVTEFYSWFGLEPM
                     LTFGTRPEKALGDEQTWRETEAMMLEALKASGVNYRVKPGDGAFYAPKIDIQIADALG
                     REWQTATIQVDRLMLPERFDLHYVDENGERQRPVAIHRAILGSLERFIGVITEHFAGA
                     FPFWCAPVQAVVIPIADRHIEAGRELGAVLGARGLHVEVDDTSNRMQNKIRLAQEQKV
                     PYMLVLGDREIEARTAAPRTRGGEQQEPVGWDDLADRLTREAADRGPG"
     gene            complement(3280..>3844)
                     /locus_tag="VMZ33_00850"
     CDS             complement(3280..>3844)
                     /locus_tag="VMZ33_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15806.1"
                     /translation="LIAALLISKTMFGVGTGVILMLAVFGRDVFGAGDLGIGILYAAR
                     GTGALIGPFLARAIFGTDDRGIIRGISVCVLAVTVSYALFPLSPTIWIAGIFVFLGHL
                     GSGAQWMLSTYGLQRATPDAIRGRIFSFEFGLVTLTIAASTIVAGILSENVAPAAAVW
                     VMVALIGIAGTAWTVFTWPLRKTAPEG"
BASE COUNT          661 a         1250 c         1267 g          666 t
ORIGIN      
        1 ggacggcacg tgggtgagga gctcttcgcc ctcgggcgac atgccaaggg cgctgccgga
       61 aatcccgtag gtagccgcgg tgccgacgtc gagcgtgaag ccgaccgtcg aggactcgcc
      121 gtagacacca acgcccgaga aggtctcagg gccttcgtcg ctctggtagg tggccaaggg
      181 gcccgtgtgg tcgccaaagg ccactatgcg cagcgcaccc gtggacgtcc cggcgcacgc
      241 gacgccgaac aacgacgtcc ctccaatggc catgtcgagg ccgacggcgt cgttgcgctc
      301 gagcgtcgtg atgtcgttgt ttgagacgtc gatgaagagc aggtcgcctt cggtggtgaa
      361 cagcgctccg accccgacat cgccgccggc atcggacgga ccgaggcaca gcagcgcagc
      421 gccgccttca gtgcttggcg tgaactcggc agcgctcagg accacgccgt actcctcgtc
      481 gagcgcgcgc tggctgtacg cgtacgccgg tgcctggagc agctcgatct gaaggctggt
      541 gacgtcgaat ccgatctccg cgaaatcggc actgcccgtg ctccagccgc tcgtcgggtc
      601 gtccatctca tcgaaaaaca aggcgtcacc ggtcgcctgg gacggtgtct caactggctc
      661 cggcgtacca gtcggctcca tactcggctg aacgctgggt gccgcggtga gggccggcgt
      721 cggctcgagg gtcggctcga gggtcggctc gagagtcggc tcgagggtag acgtggcggt
      781 cggggcggag gttggagtcg ttgccaatgt aggaatcgca cttgccgcga cggaggtggc
      841 gggagtcgcc acaccctggc ttggtgggat tgatggctgt ggcgagggca ctggcccgac
      901 ggaaacgcag cccgccgcaa cgagcgcggc aagggacatg gcgatagaaa gcttgagcaa
      961 gaccaaaccc ctcccagatc gtgacggtgt gcgggttgat cctagccgga ctcgcctctc
     1021 ctcactggct cagcctcccc gcctttcaac agccgaagcg gttcaatcgc ctggatgccc
     1081 gacagggtca agggcctacc caggtcctcg gtccgcagcc tcccgagtca gtcgatcggc
     1141 gagatcatcc cagccgaccg gctcctgctg ttcccctccc cgcgtccgtg gcgcggccgt
     1201 ccgagcctcg atctcgcgat cgccgagcac gagcatgtac ggcaccttct gctcctgtgc
     1261 cagccggatc ttgttctgca tccggttgga ggtgtcgtcg acctcgacgt gaagaccgcg
     1321 cgccccaaga accgcgccaa gctcgcgtcc ggcttcgatg tgccggtcgg caatcgggat
     1381 gaccaccgcc tgcacgggag cgcaccagaa cgggaacgcg cccgcgaagt gttcggtgat
     1441 cacgccgatg aaccgctcga gggacccgag gatcgcgcgg tggatggcaa cgggccgctg
     1501 ccgctcaccg ttctcatcga cgtaatgaag gtcgaagcgc tcgggcagca tgagccggtc
     1561 cacctggatc gtggcggtct gccactcgcg cccaagcgcg tcggcgatct ggatgtcgat
     1621 cttgggcgcg tagaacgcac cgtcaccggg cttgacgcgg tagttcacgc ccgacgcctt
     1681 gagcgcctcg agcatcatcg cttcagtctc gcgccacgtc tgctcgtcgc ccagagcctt
     1741 ttccggacga gtgccgaagg taagcatcgg ctctaggccg aaccacgagt agaactccgt
     1801 cacctcgccc atcagcgcca ggatctcgtc ctggatctgg gccggcgtca cgtagatgtg
     1861 ggcgtcatcc gtgtagaagt gtcgaacacg cgttaggccc gaagtaacgc cagacagctc
     1921 gttgcggtgg agcgcgccgt actccgacaa ccgcagcggg aactcgcggt acgagcgcac
     1981 cttggagcga tagatgaacg tcgactccgg acagttcatc ggcttgaggc tgtacgtctg
     2041 atcgtcggac tcgacaagga acatcttgtc gcggtacagg tcccagtggc ccgactgctc
     2101 ccacagcctc tggtggacga gcggcggcgt atagatctcc tggtagccgc ggcgcctctg
     2161 aatgtcgcgc atggcgtcgc gcagcgtgtt gaagaggcgc cagcccttgg ggtgccagaa
     2221 ggcgccgccg ggcgacacgt cgtggaagct gaagaggtcg agctgaacgc ccagctttcg
     2281 gtgatcgcgc ttttttgcct cttcgcgccg ccacaggaat gcatcgagct cttcctgcgt
     2341 cggccacgcc gtgccgtaga cccgctgcat ctgggggcgt ttctgatcgc cgcgccagta
     2401 ggagccggac acggcgagga gcttgatcgg cccgattttg cccgtcgatt cgacatgtgg
     2461 acctcggcac aggtcgatga atgggccctg ctcgtagaac gtcgtcttgg gcggcggcaa
     2521 gccacgactc gcggcgccag cgacgaggtc gtcgacgatc tcaaccttgt atggctgttc
     2581 cttctccagg aggaatgccc gcgcgtcggc gttcgatcgc tcgctgaaca cgaagggatg
     2641 gtcggccgcg atggactcgt tcatccgcgc ttcgatggcc gcgaggtcat ccgatgtcag
     2701 cggccgcggc agatcgaagt cgtagtagaa cccgtcctgg atgggcggcc cgatgcccag
     2761 cttcgtgccc gggaacaggt ccatcacggc ttctgccatc acgtgcgccg ccgagtggcg
     2821 cattggatcg agcgggctac ccgcactggc gtcgagcagc tcttcggccg acgttcgctc
     2881 tggcgcttcg agctcgagct cgtcttcctg ctgatcgatt gtttgttcgt cgattgccac
     2941 ggcgtcacct ctcgggaatc aaaaacccct cgtccatcga caggacgaga ggttgtgcct
     3001 cccgcggttc cacccgcctt caccatcacg tcgtccggct ggtcggcgcg acggtgctct
     3061 cagagaagtc gcgctaacgg gcgacgcccg gaccggttcg ccggtcgctc acgggtggta
     3121 cgcgcttcgc tcgaccgctt gggctttcag ccgccgaccc gagctctctg gaggtcactt
     3181 tcgcgctgcg cgtgcgtccc gatcgacgct tcctattggc cgcggagtat acgctgcgac
     3241 tggctgtgcg caatgtcgct ctcgtcctga ccgagccgct cagccttctg gagccgtctt
     3301 ccgcagcggc caggtaaaga cggtccaagc cgttcccgcg atgcctatca gggcgaccat
     3361 cacccaaaca gccgcggccg gcgcaacgtt ctcggacaag attccagcaa cgatcgtcga
     3421 cgcggcgatc gtcagcgtca cgagtccgaa ctcaaagctg aagatgcggc cgcggatggc
     3481 gtcgggcgtg gcgcgctgaa ggccgtaggt cgagagcatc cattgcgcac cggaaccgag
     3541 gtggcccagg aaaacgaaga tgcccgcgat ccagatggtc ggtgacagcg ggaacagcgc
     3601 gtacgagacg gtgacggcga ggacgcacac gctgatgcca cgaatgatcc cgcggtcgtc
     3661 cgttccgaaa atggcacgag cgagaaatgg gccaatgagc gcgccggtcc cgcgcgcggc
     3721 gtacaggatg ccgatgccca ggtcgccggc gccgaacacg tcgcggccaa agacggcgag
     3781 catcaggatg acgcccgtcc cgacgccgaa catcgtcttt gagatgagca acgcggcgat
     3841 gaga
//
LOCUS       DATLCO010000030         2015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1519681, whole genome shotgun sequence.
ACCESSION   DATLCO010000030 DATLCO010000000
VERSION     DATLCO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519681"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VMZ33_00855"
     CDS             <1..555
                     /locus_tag="VMZ33_00855"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HUR15807.1"
                     /translation="LYTRLLAEAVEERKAFVEHREPAKQRAQTVIDLPIDAYLPDEYV
                     AEEPMKLELYRRLGRTATNSEIETIRAELVDRFGSTPAPVDRLLEVARLRIAAEAAGI
                     ASLAREGHELIVRFNDDWSRSATMRAMAPTSVSDRIPGVAPGGVTYGSNQMRIRVSRD
                     AAAAWQTIRAIVDRLANRIEKTAA"
     gene            complement(601..1149)
                     /locus_tag="VMZ33_00860"
     CDS             complement(601..1149)
                     /locus_tag="VMZ33_00860"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HUR15808.1"
                     /translation="MFRNPYDFPVQVTSSGARLVDGADETIKTGSLYFLDGISGGSGY
                     LLPAETIGANVCFTCERALLTEDWAAVEFSAYIKDATDDLAYSTEVAATGVEVEFDGD
                     SPIFWITGNAENTSASTLDRISVRVIVFDKDGSLIGAAESSGWDVPAGDSVELTGGYG
                     LGENPNGPFDIEVTALGVDYSN"
     gene            complement(1487..>2015)
                     /locus_tag="VMZ33_00865"
     CDS             complement(1487..>2015)
                     /locus_tag="VMZ33_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15809.1"
                     /translation="DGATLAGNVIFQNGQDGIEIHPEAQGTRITGNQIYANGQLGIDL
                     VGGTENAAGITANDTDDPDTGANDRQNFPILTGAARNNTSGLTMVTGTLNSNPSTEFR
                     LELFLAVADASGSGEGQVLLATQNITTNANGDKSFTFASGAWSLGMVLTATATVVTAG
                     STSEFSANRTVVPGA"
BASE COUNT          331 a          657 c          635 g          392 t
ORIGIN      
        1 ctttacacgc ggttgctcgc ggaggcggtc gaagagcgca aggccttcgt cgagcaccgc
       61 gaaccagcca agcagcgcgc gcagaccgtc atcgatctgc cgatcgacgc ctatctgccg
      121 gacgagtacg tcgccgagga acccatgaag ctggagcttt accggaggct cggtcggacc
      181 gccacgaact ccgagatcga gacgatccgg gccgagctcg tcgaccgctt tggttcgact
      241 ccggcacccg tcgatcggct gctcgaggtc gcgcggctgc gtatcgccgc tgaagccgcg
      301 ggcatcgcct cgctcgcacg cgagggccac gagctcatcg tgcgcttcaa cgacgactgg
      361 tcacgctcag ccactatgcg ggcgatggcg ccgacatcgg tgtcggaccg catcccaggc
      421 gtcgcacccg gcggcgtcac gtacgggtcg aaccagatgc gcatccgcgt gtcacgggac
      481 gcggccgccg cctggcagac gatccgcgcc atcgttgatc ggctcgccaa tcggatcgag
      541 aaaaccgccg cctgaggggc cttttctttc actgcaaaga tcaggttccc gaccgagccc
      601 tcagttgctg taatccacgc caagggccgt tacttcaata tcgaatggcc cgttggggtt
      661 ttcgccgagt ccatatccgc ccgttagctc gacgctgtcc ccggcaggca catcccagcc
      721 tgagctttcg gctgcgccga tgaggctgcc gtccttgtca aagacgatca cgcggacaga
      781 gatccggtcc aatgtcgaag cgctcgtgtt ctcggcattg ccagtgatcc agaagattgg
      841 gctgtcgccg tcgaactcca cttcgacgcc tgtcgccgcc acctccgttg agtaggccag
      901 gtcatccgtt gcgtccttga tgtaggcgga gaactccacc gcggcccagt cctcggtgag
      961 cagagctcgc tcgcaggtga agcagacgtt cgcgccgatt gtctcggcgg gcagcaggta
     1021 gccgctgcca ccgctgattc cgtccaggaa gtagagcgat ccggtcttaa tggtctcgtc
     1081 cgcgccgtcg acaagcctgg cacccgacga tgtcacctgg actgggaagt cgtacgggtt
     1141 gcgaaacaag acgttagcgc gggcgttccc ctggcgatcg gtccatgatt gggattgaac
     1201 gacctcgagc gcgaggctcg cactcgtcgc ctgaggcgtt gaggcccgag gcgcggttgg
     1261 agtcgctgcc ggcgcgtcac tttcgggcgg gacctgtccg gagctcggcg gtgccgtcgg
     1321 ctcgtcagtc gggcccgcgc tcgagggttg tgtctgcccc gcgcttgttg gggccggtga
     1381 cgctggctcg gccgtcggtg ctgccgaccc gcacccagcc actgcgacga gcattagaac
     1441 aagcgtggtg aacgcgcatg ccttgttcat atcgcgatgc aatttctcag gctccgggta
     1501 ctacggtccg attagcagag aactccgacg tgctcccagc agtgacgacc gttgcggttg
     1561 ccgtcagtac catccccagc gaccaggcac ccgacgcgaa cgtgaatgac ttgtcgccgt
     1621 tcgcgttcgt ggtgatgttc tgcgtcgcga gcagcacctg cccttcgccg ctcccggatg
     1681 catcggcgac ggcgaggaac agctccagac ggaactcggt gctcggattg ctattgaggg
     1741 tgccagtgac catcgtcaga ccgctggtgt tgtttcgagc tgcccctgtc aggattggaa
     1801 agttctgacg gtcattggct cccgtgtcag gatcgtccgt atcgttggcg gtaataccgg
     1861 ccgcattttc ggtcccgccc acgaggtcga tccccagctg accattcgca tagatctgat
     1921 tgcctgtgat gcgcgtcccc tgcgcctcag ggtggatctc gataccgtcc tgcccgttct
     1981 ggaagatcac gttgccggcc agggtcgccc cgtcg
//
LOCUS       DATLCO010000031        11673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1521307, whole genome shotgun sequence.
ACCESSION   DATLCO010000031 DATLCO010000000
VERSION     DATLCO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521307"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..118)
                     /locus_tag="VMZ33_00870"
     tRNA            complement(44..118)
                     /locus_tag="VMZ33_00870"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Thr,seq:cgt)
     gene            complement(382..1596)
                     /locus_tag="VMZ33_00875"
     CDS             complement(382..1596)
                     /locus_tag="VMZ33_00875"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUR15810.1"
                     /translation="MTDPTTEATQPAGPLAGMKVLELSTVLAGPYCAMVLADLGADVV
                     KVEPPAGDATRGYGPPWIETSDGKERVAAYFLSINRNKRSVRLNLRDEKARAALGRLV
                     GWADVLIENFRPGGLDKVGLGEARLTELKPDLIHLSITGYGPTGPDARKPGYDFIAQA
                     VGGLMSVTGFAQDDSGEPTKVGVAMSDILTGLLGAVGVLAAVRSGVGQRIDVSLLEST
                     LAALINQAQNAFVSGEQPRRWGNAHPNIVPYETFATADGQIAVAVGSERQWPRLCHVL
                     GLDHLATDLRFADNDARVRNRADLRPLIANRFATQPASAWLAALDAADVPAGQVNDVA
                     AAFAQPQAVARDMRQTVAHPRLGQIQQIGLPYKLSATPASIRSAPPLLGEHTAEVLAE
                     LGFSDEEIAALT"
     gene            1648..2469
                     /locus_tag="VMZ33_00880"
     CDS             1648..2469
                     /locus_tag="VMZ33_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUR15811.1"
                     /translation="MIAPELIAFVAALGAGAFGALAGVGGGLIIVPILVALGVELHTA
                     IGVSLLGVIAVSTSASVPYLRQGFANRRLGLTLLVATAAGGIGGGYVAGLLDARALSG
                     LFALVLVLVAIQMLRARGRSPAEVVGEAGRLEVDSSYVEPTTGETITYRARNVRPALG
                     VSVIAGAVSGLMGIGGGVVNVPTMNVLMGVPIRVATATSTFMLGATAAASAVLYLSRG
                     QIDALLAAAVVSGVYVGAAAGARFSRRVSRQALSLAFIVIALIFAGQMAVRFANG"
     gene            2462..2737
                     /locus_tag="VMZ33_00885"
     CDS             2462..2737
                     /locus_tag="VMZ33_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15812.1"
                     /translation="MARGWTANALRAGGLASVAVFVIALVLHVAGLEQEATALATLGV
                     LTVIGTPSVALVATVAESLVSDRTTAWLALAVLAVLGVATVISLFLR"
     gene            complement(2935..4617)
                     /locus_tag="VMZ33_00890"
     CDS             complement(2935..4617)
                     /locus_tag="VMZ33_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUR15813.1"
                     /translation="MSDFASLVDEFLTDEFESSPVMASYLGMTAYDERLDDMSAAAFH
                     RRDSDAHAWLDRFEKAGSDDLSTDDEIDRQLGIAMMRGRLINADWQVWKRDPTTYSGP
                     ILNGLFYLFLNGLRPTADLVDAAISRLEQVPGAVEQATANLDPQLAHPLILERGAASV
                     RAGARYARDMLATEADNDSQRDLLVVAGAAAGDALEQFAAHIDALVPQAKGTWQYSGE
                     RYSAILRDRERLDHDAASLREMGQSEYDRLDAEMRELSRDIDGSDDWHAVLGKANDDH
                     PRTEEEMRRSYADWTEKARVFLAETGLVTLPDGESCSVEPAPVFTRPLIAVASYSGPP
                     AFSDQRHGHFFVPIAPDGTSEEEIQSRLASNSFGSIPTTAVHEAYPGHHWHITWSKLY
                     SPKIRRVLQTPYFSEGWALYAERVMRERGFFTEPIQELYHLEATIFRAARIVVDTSLH
                     MGEMTYDEAVKFMVEKSSLTEPTARAEVGRYCSWPTQASSYLTGCLEILRIRDRYLAA
                     RGVTYEKKSDIPVDILRDFHDRLAGSGRLPVGLAEQALMATLTDAERIAASR"
     gene            complement(4765..6255)
                     /locus_tag="VMZ33_00895"
     CDS             complement(4765..6255)
                     /locus_tag="VMZ33_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15814.1"
                     /translation="MSASAIVAFALAALLPQVVVAGGLGQSTLVRDISPGSDESGPLS
                     SDPNELTDVNGTLFFSASGPGGRELYKSDGTAQGTVRINVRPGFKSANPTSLTVLGSN
                     LFFAADGGDGHGRELWVSDGTALGTHIVKDIRAGAKSSSPAALTVMGGKVYFGANGGG
                     GLGRELWVSDGTPVGTHIVRDVIPGSTSSDPRQLVAVGTRLFFRAKSPASPTRTELWA
                     TYGMAQGTLKIPVNTGDDAAQLTRVGSQLFFISSEAPNGPFEPEWTLRVVRPGANTSV
                     RLAQLPECADPSSSSNGCHVDCGNEFCEASNEMTAVGNLLFFVTDTDRLWRSDGTRAG
                     TFKLVRLGGCNLASGVPCAKSFTDVNGTLFYVTPRIVFSNAINDYEHVGTELWKSDGT
                     VAGTAAVEVFGPYQPWYWSAPCPNCYYGSYLVGASAAFGGQYYFQGPDGALWRSDGTD
                     PGTTRACLGDDPCAYWAPARFAVSGGKLFMTADDFATGRELWRFDP"
     gene            complement(6466..7308)
                     /locus_tag="VMZ33_00900"
     CDS             complement(6466..7308)
                     /locus_tag="VMZ33_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15815.1"
                     /translation="MIGPPVRAPKLKPKAKRRKLDPVKVWTNTLNRYRPGLMEYTLDG
                     LREMYGVPAWQRFHDPTTELVLTMLTANSADINAEKAFNAMCRRWPPQPDRVNPNAQI
                     GYRAGWGGVGIQEPEADWAAVADAPLDELIDVIRPGGLGPTKAPRIQAVLRHLLTERG
                     DFSLEFLGEMAPQDALAWLTAVPGIGRKTASVVLMMAFGMPLMAVDRHVDRVAHRLGL
                     VPPKATAEQSHDYLQAQLPNELVHQAHVNLITHGRATCDAQRPACLRCLLAPRCRYVN
                     PKAV"
     gene            complement(7460..8617)
                     /locus_tag="VMZ33_00905"
     CDS             complement(7460..8617)
                     /locus_tag="VMZ33_00905"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HUR15816.1"
                     /translation="MRVLVAPDSFKGSLSSVEVARALADGWALGRPPDSVRVVPLADG
                     GEGTLEAVKAADAGWIELPVHARDPLGRPLRATFLRRGDEGIVELASASGLSRVEPSE
                     RDAMSASTFGTGLVLAAAIGLGVRSVVIGLGGSATTDGGSGLLTALGARLFDDAGRDL
                     EPGGGALSALARVDLSEVSEVLSEVRLTVASDVTNPLLGERGAAATYGPQKGATAAQV
                     ATLERNLTHYADLLESAAGRSVRDVAGAGAAGGTTAGLLAIADRFASFEIRPGVNVLM
                     ELTGFSEALADADLVLTGEGRIDEQTGFGKTAMGVARRAHDAGVQCICFGGGATPEGI
                     SALAPLGAVVVPVVEQPMTVEEAMAAGIAPVRRAAQRTARLVSLTRLVSVK"
     gene            complement(8650..10635)
                     /locus_tag="VMZ33_00910"
     CDS             complement(8650..10635)
                     /locus_tag="VMZ33_00910"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HUR15817.1"
                     /translation="MTTEANLPNRVLIPLANPRTAADLVKIGTALLAHGGTLTALGIV
                     EVAEGMPLSEGATRARQARRLLQRVLDFAPEGVELQTVVRIGRRAAEGIIELAAEDES
                     DLIIFGWGGRPGGRRGAEAQAVFSPTIDEVVRDSPCNIAVVKQRGVKSVERILTPVRG
                     GPHAELALAYASALGRYFEATVDVMHVVPSDIGPVIRSQAERALATFVQRHGGDPARP
                     LLVDGHNVGAAIIRQADEAQLVVMGASAMTPTDVAQSDGALFGVLPESIAQHAKPTVI
                     VVKTREPLIRAAFEKRAEQAETLEAADRAAEAGRSIPAKVDRWFAESNYHHTEFADLG
                     RLVALKEKRGLTISAIFPTLNESATIGPIVRSARLELMERFKLVDELLVIDSESEDDT
                     RSIAEGEGARVVVHSDVLPRYGSYKGKGEALWKSLYETSGDLVAWSDTDIQDWHPRFI
                     YGTLGPLLAEQRMGYVKAYYQRPIVEGGVLKEGGGGRVTELVARPLINLFFPELSGYI
                     QPLAGEYAGRRSDLEQIPFFTGYAVEIGHLIDLSERLGLQGFGQVDLDIRFHRNQDIE
                     GLSLMSFTILQAVMKRLEERRKARLFAELGSTMKLPRSGPGKLSLDVSELADQERPPM
                     IRIPEYIEKRSQGVPEGASLAPPREGRASTTAGRSRS"
     gene            complement(10632..>11673)
                     /locus_tag="VMZ33_00915"
     CDS             complement(10632..>11673)
                     /locus_tag="VMZ33_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall biosynthesis glycosyltransferase"
                     /protein_id="HUR15818.1"
                     /translation="SLTYPEVDGVGGKGAALRTIFQMANALKVQALVVVDSDLRSIIP
                     EWIELLAGPILKGGFDFVAPLYSRYKYDGTITNTVTYPMTRALYGLRIRQPIGGDFGV
                     SGDLVAHYLEQDDWDADVSKFGIDIWMTTKAITGGFAVCQTRLGAKVHDPKDPGADLG
                     PMFSQVLSSLLRLTDRHADRWLEVHSSHAIPAYGFERIIDPPPLEVNTRRLLDEFARG
                     YLTVKDVWSTALAPENFRIVDALAKSVDEADSFNFPDETWSRVIYDFALAYHRRLLPM
                     DQLIASLVPLYFGRVASLVVETRELTTDQAEAFVERQARAYELAKPYLVERWAEPGPA
                     AVEPAPVGAAKR"
BASE COUNT         1884 a         3811 c         3960 g         2018 t
ORIGIN      
        1 ccccctgacc ccttcattcg ctgcttgctg ggtgcgccgc gaatggagcc gacgctcgga
       61 tttgaaccga ggacctgctg tttacgaaac agctgctcta ccgctgagct acgtcggcgt
      121 gcgccgaagt gtaccccgcc gccttcgagc gccccgatgg gcgatttggc gtccgcgtcg
      181 ttgggcatat gtatcgcgct ggacgggagt tgcacgtggg cacgagctcc ttggagccac
      241 tggttggaga cggttggcgg ggaccagatg agaatgacac cccgtatcgt ggtgtcaaga
      301 cgcaaagcat aacaattagg acgaaggtcg ctcgggcagt gggcgagcgg gttgagctgg
      361 tggccgtggc cagcgggcgg ctcaggtaag cgcggcgatc tcttcgtcgc tgaagccgag
      421 ctcggccagc acctcggctg tgtgctcgcc gagcagcggc ggagccgagc ggatggaggc
      481 cggcgtagcg gacagcttgt acggcaggcc gatctgctga atctggccga gacgtgggtg
      541 ggcgacggtc tggcgcatgt cgcgggccac cgcctgtggc tgagcgaacg ccgcggctac
      601 gtcgttgacc tgcccggccg ggacatccgc cgcgtcgagc gcagccagcc acgcgctcgc
      661 tggttgtgtg gcgaaacgat tggcgatcag tggccggagg tcggcccgat tgcgcacacg
      721 ggcatcgttg tcggcaaagc gcaggtccgt agcgagatgg tcgagtccca ggacgtggca
      781 cagccgcggc cattggcgct cgctgccgac ggcgacggcg atctggccgt cagccgtcgc
      841 aaaggtctcg tacgggacga tgtttgggtg cgcgttgccc caacggcgcg gctgctcacc
      901 cgagacgaaa gcgttctgcg cctggttgat cagcgctgcc agggtcgact cgaggaggga
      961 cacatcgatt cgttggccga cgccggatcg aaccgcggcg aggaccccga cagctcccag
     1021 caggccagtg aggatgtcgc tcattgcaac accgaccttc gtcggctcgc cactgtcgtc
     1081 ctgcgcgaac ccggtcacgg acataagccc gccgacggcc tgggcgatga agtcgtagcc
     1141 cggcttccgg gcgtcgggac cggtcggtcc gtaacccgtg atcgagaggt ggatgaggtc
     1201 tggcttcagc tcagtcagtc gggcttcgcc aaggccgacc ttgtcgagcc caccgggcct
     1261 gaaattctcg atcagcacgt cggcccagcc gacgagccgg cccaaagctg cacgagcctt
     1321 ttcgtcgcgt aggttcaggc gaacgctgcg cttgttgcgg ttgatcgaga ggaaatacgc
     1381 ggccacgcgt tccttgccgt cgctcgtctc aatccaaggc gggccgtatc cgcgggtagc
     1441 atcgcctgcc ggtggttcga ccttcacgac gtcggcgccg agatcggcaa gcaccatcgc
     1501 gcagtaggga ccggccagga cggtcgagag ctcgagaacc ttcataccgg ccaatggtcc
     1561 ggctggttgg gtcgcctcgg tcgtcggatc agtcacgtgg gcgatcgtac ctgtggcggg
     1621 atcggctgca gcccataatc ggcgtcagtg attgcccctg agctgatcgc cttcgtggcc
     1681 gcgctggggg cgggcgcgtt tggggcgttg gccggcgtcg gcggtggctt gatcatcgtg
     1741 ccgatactcg ttgccctcgg cgttgagcta cacacggcga ttggcgtgag cctgttgggc
     1801 gtcatcgccg tttcgacgag cgcctcagtg ccgtacctgc ggcagggatt tgccaaccgc
     1861 cgccttgggc tgactctcct ggtcgcgacc gccgccggcg gtatcggtgg tggctacgtg
     1921 gccgggctcc tcgacgcacg tgccctgagc ggactcttcg ccctggtgct ggtgttggtc
     1981 gcgatccaga tgctccgcgc gcgcgggcgg tctccggcgg aagtcgtggg cgaggcgggc
     2041 aggctggagg tcgactcaag ttacgtggag ccgacgacgg gcgagacgat cacctaccgc
     2101 gcgcggaacg tgcgacccgc cctcggtgtg tcggtcatcg cgggcgccgt gtcagggttg
     2161 atggggatcg gcggcggggt ggtcaacgtg ccaacgatga acgtcctcat gggggtgccc
     2221 atccgcgtgg ccacggccac gagcacattc atgctcggcg cgacggccgc cgccagcgct
     2281 gtcctgtacc tgagccgcgg gcagattgac gcgcttctcg cagcggcggt ggtgtcaggc
     2341 gtctacgtgg gtgccgctgc aggcgcgcgc ttctcgcgac gtgtgtcacg gcaggcactc
     2401 agcctcgctt tcatcgtcat cgcgttgatc tttgcggggc agatggcagt gcggttcgcc
     2461 aatggctagg ggctggacgg caaacgcgct gcgcgccggc gggcttgcct cggtcgcggt
     2521 gttcgtcatt gccctggtgc ttcatgtggc agggctcgag caggaggcca ctgccctggc
     2581 gaccctgggg gtactgaccg tgatcgggac accgtccgtg gcactcgtcg cgaccgttgc
     2641 cgagtcactg gtttccgacc ggactacggc gtggctggcc ctggccgtgc tggccgtgtt
     2701 gggggtcgcg acggtcatct cacttttcct gagataacga gaacggcccg gggagggggc
     2761 ccgggccgtt ctatatcgat gcgggagcgc cccgtcacca gacgcttcgc attggtgttg
     2821 gctcttgtgg atcgaactct aggacgcgac caccatggcg ggccattgcg ccgcccaatg
     2881 tgactcttgc agtcctatat cgcggtcaga gacggcttcg ccgacccgcc ggcgctatcg
     2941 acttgctgcg atgcgctccg cgtcggtgag cgtcgccatc agcgcctgct cggccagccc
     3001 gacaggcagt cgaccgctcc cggcgaggcg gtcgtgaaag tcgcgcagga tgtcgaccgg
     3061 aatgtccgat ttcttttcgt acgtgactcc tcgggccgcc aggtagcggt cgcggatcct
     3121 gaggatctcg aggcaaccgg tgagatacga cgaggcctgc gtgggccagg agcaatagcg
     3181 tccgacctcc gcccgggcgg tcggctcggt cagcgagctc ttctcgacca tgaatttgac
     3241 ggcctcgtcg taggtcatct cgcccatatg gaggcttgtg tcgacgacga tgcgagcggc
     3301 gcgaaaaatc gtcgcctcga ggtgataaag ctcctggatc ggctcggtga agaagccacg
     3361 ctcgcgcatg acgcgctcgg cgtagagcgc ccagccctcg ctgaagtagg gcgtttgaag
     3421 gactcgccgg atcttgggtg agtagagctt gctccacgtg atgtgccagt ggtggcccgg
     3481 gtatgcctcg tggacggcgg tcgttgggat ggacccaaac gagttcgacg cgagcctgct
     3541 ctggatttcc tcctcactcg tgccgtcggg cgcgatcggg acgaagaagt gcccatgtcg
     3601 ctggtcggag aaggccggcg gtccgctgta cgacgcaact gcgatgagcg gccgcgtgaa
     3661 gaccggggcg ggctcaaccg agcacgactc accgtcgggg agtgtcacca gccctgtctc
     3721 ggccaggaag acccgcgcct tctcagtcca gtcggcgtag ctgcggcgca tctcctcctc
     3781 agtgcgcggg tggtcgtcgt tcgccttgcc cagcacagcg tgccaatcgt cgctgccgtc
     3841 gatgtcgcgc gaaagctcgc gcatctcggc gtccagccgg tcgtattcgg attggcccat
     3901 ctcacggagc gacgcggcgt catggtcgag ccgctcgcgg tcgcgaagga tcgcgctgta
     3961 ccgctccccg gagtactgcc acgtgccctt ggcctggggg accagcgcat cgatgtgcgc
     4021 tgcgaactgc tcgagcgcat cgcccgccgc cgcgcctgcg acgaccagca ggtcgcgctg
     4081 cgaatcgttg tcggcctcgg tcgcgagcat gtctctggcg tagcgcgcgc cggcacgaac
     4141 ggaggcagct ccacgctcaa ggatcagcgg gtgggcgagc tgtgggtcca ggtttgctgt
     4201 cgcctgctca acggcgcccg ggacctgctc gaggcgcgag atggcggcgt ccactaggtc
     4261 ggcggtggga cgcaggccgt tgaggaacaa gtagaagagt ccgttgagga ttgggccgct
     4321 gtacgtggtg gggtcgcgct tccagacctg ccaatcggcg ttgatgagcc ggccgcgcat
     4381 cattgcgatg ccgagctgcc ggtcgatctc gtcgtccgtc gagagatcgt cgctgccggc
     4441 cttctcaaat ctgtccaacc atgcatgcgc gtcggagtca cgtcgatgga acgcggcggc
     4501 cgacatgtcg tcgagccgct cgtcgtacgc agtcatcccg aggtagctcg ccatgactgg
     4561 cgacgactcg aactcgtcgg tcaggaactc atcgacgagt gatgcaaagt cggacatgtt
     4621 tctcctcagc acaggggggc gcgtgtcacc ttaattgcca cctcgccttg tcgcctgatc
     4681 gaggcgagcc cgcgaacagc atctccctgc tatccgacga cgatgccccc tcccgaagcc
     4741 ggaccaacgc gtccccctca ccgactaggg atcgaaccgc cagagctcgc ggcccgtcgc
     4801 gaagtcatcg gcggtcatga agagcttgcc tcccgaaacc gcgaagcgtg ccggagccca
     4861 atacgcgcat gggtcgtcgc cgaggcacgc ccgcgtcgtt cctggatcgg tgccatcgct
     4921 gcgccagagc gcgccgtctg gtccctggaa gtagtactgc cctccgaacg cggcactcgc
     4981 gccaacgagg tacgacccgt agtagcaatt ggggcatgga gcgctccaat accagggctg
     5041 ataaggccca aagacctcga ccgcggccgt accagccact gtgccgtcgc tcttccagag
     5101 ctccgtgccg acgtgctcgt agtcgttgat ggcgttcgag aacacgatgc gcggcgtgac
     5161 gtagaagagg gtgccgttga cgtcagtaaa cgacttggca cagggcacgc cactcgcgag
     5221 attgcagccg cccaaccgga ccagcttgaa cgtgccggcc ctggtgccat cgctgcgcca
     5281 caggcgatcg gtgtctgtca cgaagaacag caggttgccg acggcggtca tctcgttgct
     5341 cgcttcgcag aactcgtttc cgcaatcgac gtgacaacca ttcgagctcg acgacggatc
     5401 agcacactcc ggcagttgag ccagccgaac cgacgtgttc gcgcccggac ggacgactcg
     5461 caacgtccac tctggctcga agggcccgtt gggcgcctcg gagctgatga agaacaattg
     5521 cgagccgacc ctcgtcagct gcgcggcgtc gtcaccggta ttcacgggta tcttgagtgt
     5581 gccttgcgcc atgccgtacg ttgcccatag ctcagtcctg gtcgggctgg cgggtgactt
     5641 ggcgcggaaa aacagacgag ttcccacagc gaccagctgc cgcgggtcgg aactcgtgct
     5701 tcccggaatc acatcacgca cgatgtgcgt gccgaccggc gtgccgtcgc tgacccacag
     5761 ctcacgtccc agtccgccgc cgccgttcgc gccgaaatag accttgccgc ccatgacggt
     5821 cagggccgcc ggcgaagagc tcttggcgcc ggcacggatg tccttcacga tgtgtgtacc
     5881 gagcgcggtc ccatcgctca cccacagctc ccgcccgtga ccgtcgccgc cgtcggcggc
     5941 aaagaacaag ttcgagccga ggacagtgag ggaagtgggg ttggcgctct tgaagccggg
     6001 ccgtacgttg atgcggaccg tgccttgcgc agtcccatcg ctcttgtaga gctcgcggcc
     6061 gccggggcct gaggcgctga agaacagcgt gccgttgacg tccgtaagct cgttgggatc
     6121 ggagctcagt gggccggact catctgagcc gggtgagatg tcgcgcacga gggtgctctg
     6181 gccgagcccg ccagcgacga cgacttgggg gagcagtgcc gcgagtgcga acgcgacgat
     6241 tgccgatgct gacattgagc gagatttggg cattcctcat gcctccctgg gcttcgcgca
     6301 gatccccccg gtggctgacg ctctgcgcgc cggctttcag tcgcgggtca ccgtaccccc
     6361 atcaccgaat cacggcatgg gggcatctac gggcgaacct acgggtttga tcctaggtag
     6421 ggtcggtagg ttctgggtac cgctaacccg gtcgggccct aacccctaga cggccttcgg
     6481 gttcacatag cggcagcgcg gggcgagcag gcagcgcagg caagccgggc gctgcgcgtc
     6541 gcacgtcgcc cggccgtgcg tgatcaggtt gacgtgcgcc tggtgcacga gctcgttggg
     6601 tagctgcgcc tgtaggtagt cgtgcgactg ctcggccgtc gccttgggcg ggaccaggcc
     6661 caggcgatgg gcgactcggt cgacatgccg gtcgacagcc atcaggggca tgccgaacgc
     6721 catcatcagt acgaccgacg ccgtcttccg tccgatgccc ggcacagcgg tcagccaggc
     6781 gagcgcgtcc tggggcgcca tctcgccgag gaactccagc gagaagtcgc cgcgctcggt
     6841 gaggaggtgg cgcaggacgg cctggatgcg tggcgccttt gtcgggccaa ggccacccgg
     6901 ccggatgacg tcgatcagct cgtccagtgg cgcgtcggcg accgccgccc aatcggcctc
     6961 cggctcctgg atgccgacac ctccccagcc ggcccgataa ccgatctgag cgttgggatt
     7021 cactcggtca ggctgcggcg gccaacggcg gcacatggcg ttgaaagcct tctcggcgtt
     7081 gatgtcggcg ctgttggcgg tgagcatcgt tagaacaagc tcggtcgtcg ggtcgtggaa
     7141 gcgttgccag gcggggacgc cgtacatctc acgcagcccg tccagtgtgt actccatgag
     7201 ccctggccga taccgattga gcgtgttcgt ccagaccttg accgggtcga gctttcggcg
     7261 cttcgccttc ggcttcagct ttggggctct gacgggtggc ccaatcattt ggacgccaca
     7321 gcgttgcgca gttcgtggcg aacgacggaa cttcccgcgc cggcacgcac acgcgtgcgg
     7381 ttgcttgggc aagccgcgca ccggaacgcg ggagcgcgca attgcgtgga tttccgaaag
     7441 cgacaaaacg gacgcgcgtt catttgactg aaaccaggcg ggtgagtgaa accaggcggg
     7501 ccgtgcgttg ggcggcgcgg cggacgggtg ctatgccggc ggccattgcc tcctcgacgg
     7561 tcatcggttg ttcgacgact gggacgacga ctgcgccgag tggcgctagc gcggaaatcc
     7621 cctcgggcgt ggccccgccc ccgaagcaga tgcactgcac gccggcatcg tgcgcgcgtc
     7681 gtgcgacgcc cattgcggtc ttgccaaagc cggtctgctc gtcgatgcgc ccctccccgg
     7741 tgagaaccag gtcagcgtcg gcgagcgcct cggagaagcc ggtcagctcc atcagcacgt
     7801 tgacgccggg gcggatctcg aacgaggcga agcggtcggc gattgccagc aatcctgcgg
     7861 tcgtgccgcc ggccgcaccc gcacccgcga catcgcgtac cgaccgaccc gcggccgact
     7921 ccaggagatc ggcgtaatgc gtgaggttgc gctccaaggt cgccacctgg gcggcagtcg
     7981 cgcccttctg tgggccatac gtcgcagctg ctccgcgctc gccaagcaac ggattggtga
     8041 cgtccgatgc gacggtcagg cgcacctcgc tcagcacttc cgagacctcg ctaaggtcga
     8101 cacgcgccaa tgccgagagc gcgccaccgc ccggctcgag atcccgcccg gcgtcatcga
     8161 acaaccttgc tccgagggcc gtcagcagac ccgacccacc gtcggtggtt gcgcttccac
     8221 caagaccgat aacgaccgac cggacgccca gcccgattgc cgcggccaac accaggccgg
     8281 ttccgaacgt agaagcggac atcgcatcac gctccgacgg ctccacccgc gacaaacccg
     8341 acgccgacgc cagttcgacg atgccctcgt cgccacgccg caggaaggtg gcgcggagcg
     8401 gccggcccaa tgggtcgcgc gcgtgaactg gcagctcgat ccaaccggcg tctgcggcct
     8461 tgacggcctc gagggtgcct tcgccgccgt cggccaacgg cacgactcgg acagagtcag
     8521 gtggacgccc caatgcccag ccgtcggcca gcgcgcgggc cacctcaacc gacgagaggg
     8581 agcctttgaa agaatccggc gcgaccaata ccctcaagaa tcaggccctc acgtcctgct
     8641 gaggtcgcct tagcttcgcg atctgccggc cgtcgttgat gcgcggccct cacgaggtgg
     8701 cgccaagctt gccccctcgg gcaccccttg cgatcgcttc tcgatgtact ccgggatgcg
     8761 gatcatcggt gggcgttcct gatcggctag ctcagaaacg tcgagcgaca gcttgcccgg
     8821 gccggagcgc ggaagcttca tggtcgaccc cagctccgcg aaaagtcggg ccttccgccg
     8881 ttcttcgagg cgcttcatga ccgcctggag gatcgtgaag ctcatgagcg acaggccctc
     8941 gatgtcctga ttgcggtgaa atcggatgtc gaggtcgacc tggccgaagc cttggaggcc
     9001 cagcctttcc gacaagtcga tcaggtgtcc gatctctacc gcgtagccag tgaagaacgg
     9061 gatctgctca aggtctgaac gtcggcccgc gtactccccg gcgagtggct ggatgtatcc
     9121 cgacagctcc gggaagaaca ggttgatcag cggtcgtgcg acgagctccg tcacgcgtcc
     9181 gccgccgccc tccttgagca cgccgccctc gacgattggt cgctggtagt aggccttcac
     9241 gtagcccatg cgctgctcag ccagcagggg acccagcgtg ccgtagatga aacgcgggtg
     9301 ccagtcctgg atgtccgtgt cggaccatgc gacaagatcg ccggacgtct cgtagagcga
     9361 cttccagagc gcctcgccct tgcccttgta cgagccgtag cggggcagga cgtcggagtg
     9421 cacgaccacg cgggcaccct cgccctcagc gatggagcgt gtgtcgtcct ccgactccga
     9481 gtcgattacc agcagctcgt cgacgagctt gaagcgctcc atgagctcaa ggcgtgctga
     9541 acggacgatg ggtccgatcg tcgcgctctc gttcagagtc gggaagatcg cgctgatcgt
     9601 caggccgcgc ttctccttga gagccacgag gcggccgagg tcggcgaact cggtgtggtg
     9661 gtaattcgac tcggcgaacc agcggtcgac cttggcgggg atgctgcggc cggcctcggc
     9721 cgcgcgatcg gcggcctcga gagtctccgc ctgctcggcg cgcttctcga acgcggcacg
     9781 tatcagcggc tcgcgcgtct tgaccacgat gacagtcggc ttggcgtgct gagcgatcga
     9841 ctcaggaagg acgccgaaca gcgcgccgtc ggactgcgca acgtcggtag gcgtcatcgc
     9901 cgacgcgccc atgacgacga gctgagcctc gtccgcctga cggatgattg ccgcaccgac
     9961 gttgtgcccg tcgacgagca gcggccgtgc cggatcgccg ccgtggcgtt gcacgaacgt
    10021 ggccagggca cgctccgcct gggagcggat gacggggccg atgtccgacg ggacgacgtg
    10081 catgacgtcc accgtggcct cgaaatagcg gccgagagca gacgcgtacg caagcgcgag
    10141 ctcggcgtgt ggaccaccgc gcacgggggt caggatgcgc tcgaccgact tgacgccgcg
    10201 ctgcttgacc accgcgatgt tgcacggcga gtcgcgcacg acctcgtcaa tggtcggcga
    10261 gaacacggcc tgggcctctg cgccgcgacg gccaccgggc cgcccacccc agccaaagat
    10321 gatcaggtcc gactcatcct cagccgcgag ctcgatgatt ccctcagccg cgcggcgccc
    10381 gatccgcacg acggtctgga gctcgacccc ttcgggggcg aaatcgagaa cacgttgcag
    10441 cagtcgacgc gcctggcgcg cccgagttgc gccctcggac agtggcatgc cctcggcaac
    10501 ctcgacgatg cccagcgcgg tcagcgttcc tccgtgagcc aggagcgctg ttccgatctt
    10561 caccaggtcc gccgccgtgc gcgggttcgc caacgggatc aagactcggt tcgggaggtt
    10621 tgcctccgtc gtcatcgctt cgcggccccg acgggcgcgg gttcaaccgc cgccggcccg
    10681 ggctctgccc agcgctcgac gagatatggc ttggccagct cgtacgcccg ggcctggcgc
    10741 tcaacgaaag cctcagcctg gtcggtcgtc agctccctcg tctcgacgac aagactcgcg
    10801 acgcgtccga agtacagcgg caccagtgac gcgatcagct ggtccatggg cagcaaccgg
    10861 cggtggtagg cgagggcgaa gtcgtagatc acgcggctcc acgtctcatc ggggaagttg
    10921 aagctatccg cctcatcgac gctcttcgcg agcgcgtcga cgatgcgaaa gttctctggc
    10981 gcgagtgcag tcgaccacac gtccttgacc gtcaggtagc cgcgcgcgaa ctcgtctaga
    11041 aggcgccgcg tgttcacttc cagcggcggc gggtcgatga tccgttcgaa gccgtaggct
    11101 gggatcgcgt gcgaagagtg cacctcgagc cagcgatcgg cgtggcgatc ggtcagtcgg
    11161 agcagcgacg agaggacctg gctgaacatc gggcccaggt cggcaccggg atccttgggg
    11221 tcgtgcacct tggctccgag tcgcgtctgg cagaccgcaa atccgcccgt gatcgccttg
    11281 gtcgtcatcc agatatcgat gccgaacttc gagacgtccg cgtcccagtc gtcctgctcc
    11341 aggtagtgcg ccacgaggtc gccactgacc ccgaagtcgc caccgatcgg ctggcggatg
    11401 cgcagcccgt agagagctct ggtcatgggg tatgtgacag tgttggtgat cgtgccgtcg
    11461 tacttgtagc gcgagtagag cggcgccacg aagtcgaagc cccctttcag gatgggcccg
    11521 gcgaggagct cgatccactc ggggatgatc gagcgcaggt cggagtcgac aacgaccagc
    11581 gcctgcacct tcagggcgtt cgccatctgg aagatcgtgc gcagcgcggc gcccttgccg
    11641 ccgacgccgt cgacctccgg gtacgtcagg gag
//
LOCUS       DATLCO010000032         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8213000, whole genome shotgun sequence.
ACCESSION   DATLCO010000032 DATLCO010000000
VERSION     DATLCO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8213000"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VMZ33_00920"
     CDS             <1..235
                     /locus_tag="VMZ33_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15819.1"
                     /translation="AAAAATDPGRATRILAAAAAMRDRTGARLSLASQGSVDQQLARL
                     QTALAGDFERVWQQGSLASVDDALRDAAAVVGR"
     gene            complement(264..515)
                     /locus_tag="VMZ33_00925"
     CDS             complement(264..515)
                     /locus_tag="VMZ33_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HUR15820.1"
                     /translation="MPTYLDTHHHIPGLTAEALAQAHARDVEVGASYDVRYVRYWFDE
                     ATGRVYCLVEAPSPELANQVHREAHGLVADEIVPVSEGS"
     gene            665..1063
                     /locus_tag="VMZ33_00930"
     CDS             665..1063
                     /locus_tag="VMZ33_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUR15821.1"
                     /translation="MQYALLIYVAPWEPTEEESAEVMKSYNAFTADIVASGVMRGGEA
                     LEDANTATSVRVRNGQTLVTDGPFAETKEELGGFYVVEAESLDEAVKWAAKIPGAARG
                     TIEIRPIWQVPNMGEVSEHEHESAALGAAG"
     gene            1038..>2170
                     /locus_tag="VMZ33_00935"
     CDS             1038..>2170
                     /locus_tag="VMZ33_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUR15822.1"
                     /translation="MPRWAPLAEPGARGGIGPTGPFAFDAVDRIFREESGRVVAGLIR
                     SVGDFDLAEESVQDAFIEALEHWPTSGIPPNPGGWIAMTARRKAVDRLRRSKRLASKV
                     DILGQLTTVESERLAPSPEETEDRPDIEDDRLRLMFTCCHPALAPDAQVALTLRMLGG
                     LRTPEIARAFLLPEPTLAQRLVRAKKKIRDAGIPYRVPPAEELPERLESVLHVLYLIF
                     NEGYSATAGATVVRRDLTSEAIRLGRVLASLMPSEPEVWGLLALLLLHDSRRDTRQTP
                     EGDVVLLRHQDRSRWDRSRIEEGSQALERAASLGVVGPYQVQAAISAVHARAATHDRT
                     DWRRIVTLYDSLLALAPSPVVELNRAVAVAMADGPDLGLALID"
BASE COUNT          358 a          698 c          756 g          358 t
ORIGIN      
        1 ggcagccgca gccgctaccg acccgggtcg cgcgacacgg attcttgcgg ccgcggccgc
       61 gatgcgcgac cggaccggtg cgcggctatc cctcgccagt cagggatcgg tcgatcagca
      121 gctggcgcga ctccagacgg cgcttgccgg cgatttcgag cgtgtgtggc agcagggcag
      181 cctcgcatcg gtcgacgacg cgctgcgcga cgcggccgcg gtcgtcggcc gctgatcaca
      241 acgtcatgcg gaaaacgcgc gaactaggag ccctctgaga cgggcacgat ctcgtccgcg
      301 acgagcccgt gcgcctcgcg gtggacctga ttggccaact cggggcttgg cgcttcgacg
      361 aggcagtaga cacgaccggt tgcctcgtca aaccaatagc gaacgtagcg cacgtcgtag
      421 ctcgcgccta cctcgacatc gcgtgcatga gcctgcgcca gggcttcggc ggtcagcccc
      481 ggaatgtggt gatgcgtatc gagatacgtg ggcatccgtt ctccccggtc acggctgtgg
      541 tggtgcttgg gcgggaatat tagagccgag catcgccgcc ggtcgaaaaa cgcagaggcc
      601 gttcgttgtc ttgtcgacgg cggacgcatc gccgaagatc gcagtctcag gaagggacaa
      661 caaaatgcag tacgcgttgc tcatctacgt cgcgccatgg gagccgaccg aagaggaaag
      721 tgcggaggtc atgaagtctt acaacgcctt cacggccgac atcgtcgcgt cgggcgtcat
      781 gcgcggcggg gaagccctcg aggacgccaa cacggcgacc tcggtccggg ttcggaacgg
      841 ccagaccctc gtcaccgacg ggcccttcgc cgagaccaag gaagagctgg gtggctttta
      901 cgtggtcgag gctgagagcc tggacgaggc cgtcaagtgg gccgcgaaga tccccggggc
      961 ggcccgcggc acgatcgaga tccggcctat ctggcaggtc ccgaacatgg gcgaggtcag
     1021 cgagcacgaa cacgaaagtg ccgcgctggg cgccgctggc tgagcctggc gctcgcggtg
     1081 gcatcgggcc aacaggtccg tttgcgttcg atgccgtcga ccgcatcttc cgcgaggagt
     1141 cgggccgcgt cgtagccggt ctgatccggt ccgtcgggga cttcgacctc gccgaagagt
     1201 cggtgcagga cgccttcatc gaggcgctcg agcactggcc gacctctggc atcccgccca
     1261 atccgggcgg ttggatcgcc atgaccgcac gccgcaaagc cgtcgaccgc ctgcgtaggt
     1321 caaagcggct cgcttcgaaa gtggacatcc tcggacagct gacgacggtt gaatcggaac
     1381 ggctggcgcc gtcgcccgag gagacggagg atcgacccga tatcgaggac gaccggcttc
     1441 gactgatgtt cacctgctgc catcccgccc tcgcacccga cgcccaggtg gcgttgacat
     1501 tgcgaatgct gggtggcttg cggacgccgg agatcgcgcg cgccttcctg ctgcccgagc
     1561 cgacgttggc ccaacggctg gtccgcgcca agaagaagat ccgcgacgcc ggcattccct
     1621 accgcgtgcc acctgccgag gaactgccgg agcggctcga gtcggtcctg cacgtcctgt
     1681 acctgatctt caacgagggg tactcggcga ccgccggcgc aaccgtcgtc cgtcgcgacc
     1741 tgacgtcaga ggcgatccgg ctggggcgcg tgctggcgtc gctgatgccc agcgagccgg
     1801 aggtatgggg gctgctggcg ctgctgctgc tccacgactc gcggcgcgat acgcggcaga
     1861 caccggaagg cgatgtggtg ctcctgcgcc atcaggaccg gtcgcgctgg gatcggagtc
     1921 gaatcgagga gggctcgcaa gcccttgagc gtgccgcgtc tctgggtgtc gtgggtccat
     1981 atcaggttca ggcggcgatc tctgccgttc atgcccgagc tgccactcac gaccggaccg
     2041 attggcggcg catcgtgacc ctctatgact cgctccttgc gctggcgccg tcgccggttg
     2101 tggaattgaa cagggcggtg gctgtcgcga tggccgatgg gccggatctt gggctggcgt
     2161 tgatcgacga
//
LOCUS       DATLCO010000033         5900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1522539, whole genome shotgun sequence.
ACCESSION   DATLCO010000033 DATLCO010000000
VERSION     DATLCO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522539"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1554)
                     /locus_tag="VMZ33_00940"
     CDS             complement(13..1554)
                     /locus_tag="VMZ33_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15823.1"
                     /translation="MRLHHLVASLIVISLTGCAYVGETSPSPSAVASPTNAPPATTGA
                     PVPVATFTQAPVLSAAPQTSTSPTQQPTPTPTLEPAPTTEPSRTGWQRIPDFPPSNAR
                     VRALTAFDGGFVAVGGSGATDACDSATQARIWTSPDGLSWSAASLDLDRLSLFDVAVA
                     GGAVYAFGSEQSRGCSIERYVVARSTNGSEWRIDDVQTHGDQYFNDFVGVGDRLLGFG
                     EAYDEEGRALGSVWISDDGIDWRVSQDNIESGFELESAVAVGDTVALADYAARPIWLS
                     TDAGDTWRRAEFGPMYAAYPLRLAASDARFVAVGHACCMAPDDTVGFAINSEDGNSWS
                     ESEPFGFRQIPDAIVGLADGFVTIGRQSWLSQDGSSWVIGPDLPGYEIDPDYYLPSGA
                     LSGDRVVVVNRTQAWVASASELDPSGFSDTPRLAERPAIGSSWQYDAFTHCGWPDVHV
                     DLRAWVPDPPFHDPNGNPPQGVKEFDHGTLTFVSQNRLEYEAESGQIIVLVPSDEPTL
                     SGPCA"
     gene            2126..3244
                     /locus_tag="VMZ33_00945"
     CDS             2126..3244
                     /locus_tag="VMZ33_00945"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HUR15824.1"
                     /translation="MPTVTWEQALAWRMNRHLLEPVGNLGPEDVVRRLCGVQAQVSSS
                     ADFAIRARQANSEAGAVAEALADGRLVKTWAMRAALHLLTPEDAGDYMGLMAAGRPWQ
                     RPSWQRYFGLTPTEMDNLRDAVIEILGNRAMTREELTAEVTARPGFAHIGQGLKSGWG
                     MLFKPIAWQGGLIHGPSQGTRITFIRPQVASRLWRPPPDPDEAGPRVLERYLAAYGPA
                     TLNNFAAWLSRGTIAPTRVKRWVLELGDRLTAVDVSGTATYLRSEDVDDLASTKSSNA
                     VRLLGGFDTWVLGPGTDDTHVIPAARRNAVSKTAGWIAPIVVVGGVVRGTWEMGMGTV
                     SVSWWKESGKPPSRAIEVEAARIGRIRGEKLQVAISTD"
     gene            3641..4372
                     /locus_tag="VMZ33_00950"
     CDS             3641..4372
                     /locus_tag="VMZ33_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15825.1"
                     /translation="MRAIKVFHALRAVVILGALVGALAAPGAVGAASRDSDGDGLRNA
                     YETNWSHTNPRDRDSDNDGKSDARENPDQDGLSNRQEQRLALNPLDSDSDNDNVEDGD
                     EDPDDDGLDNEDEFDAHTNPTDDDSDNDGVEDDDDDSDCDELSNDTELGAGLKPFDAD
                     SNNDGDEDGDEDKDHDGVQNEDEEDNGTDIDDEDSDDDNIDDGDDEDGDGDGDDDDAD
                     DDNDEDVEDPDCGDEDEDEGGEDED"
     gene            4570..5151
                     /locus_tag="VMZ33_00955"
     CDS             4570..5151
                     /locus_tag="VMZ33_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUR15826.1"
                     /translation="MPEPSASGLHDRRIVDAVLAGDRDSFRALVERESATVIAVCMSV
                     LRDREEALDVAQDAFVQAYRALATYRGDGTFGAWIARIASRLAVARASTTARRPVTTT
                     DDINAYDVADVSGDGFDLERTVLGEERAAALRDAIERLPADQREVVNLRFFRDMPLEQ
                     IALETASPLGTVKSRLHRALQRLREQADLGSAS"
     gene            5148..>5900
                     /locus_tag="VMZ33_00960"
     CDS             5148..>5900
                     /locus_tag="VMZ33_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15827.1"
                     /translation="MTAASIEFDSWFVDAVMDRVARERVPTPANTLRAAVRGLDLSMA
                     LASAWAAWQLATTGGFVQRRVRLRSAMLLATLLLIAAMGTTLAAAGTAVVVQTAAEHL
                     SRVMIQTPPGEPSLPGVVLVEPGDSPQPMTTREPAPRTADPGQDPTRQPPVVPWQSHS
                     PSPRTVGQPERSTATPSRSPQPTEAATPTDAPNPTRTPRPTPSATPTDRPSGGDDQAP
                     REDGGGDADDGADPPGDDGNDGGGDADDGDDPP"
BASE COUNT         1089 a         1878 c         1999 g          934 t
ORIGIN      
        1 cgctcgcgga ggtcaggcgc agggacctga cagggtcggc tcatcactcg gaacgaggac
       61 gatgatctgg ccgctttcgg cctcgtactc gagccggttc tggctgacga acgtcaatgt
      121 gccgtggtcg aattccttga caccttgcgg cggattgccg ttgggatcgt ggaacggcgg
      181 atcggggacc caggccctta gatcgacgtg aacgtcgggc cacccacagt gggtgaacgc
      241 gtcgtactgc cagcttgagc cgatcgctgg gcgctcagct agacgtggcg tgtccgagaa
      301 tccggacgga tcgagttcgc tggctgacgc gacccaggcc tgtgtccgat tgaccaccac
      361 gacccgatca cccgaaaggg cgcccgaagg caagtagtag tcggggtcga tttcatagcc
      421 tggcaggtcg gggccgatga cccagctaga cccgtcctgc gacagccaac tctgacgacc
      481 gatcgtcacg aatccatcag ccaagccgac tatcgcgtcc gggatttgac gaaagccgaa
      541 cggctcggac tcgctccagg agttcccgtc ctccgaattg atggcaaagc caaccgtgtc
      601 gtctggcgcc atgcaacagg cgtgaccgac cgcgacgaac cgtgcgtcgg acgccgctag
      661 acgcagtggg taggcggcat acatgggacc aaactcggcc cgccgccacg tatctccggc
      721 gtccgttgac agccagatcg ggcgggcggc gtagtcggcg agagccactg tgtcccccac
      781 ggcaaccgcg ctctcaagct cgaaaccgct ctcgatgttg tcctggctga ctcgccagtc
      841 gatcccgtcg tcggaaatcc agacggaccc gagggcgcgg ccctcttcgt cgtaggcctc
      901 gccaaatccg agaaggcggt cacctacgcc tacgaaatcg ttgaagtact gatcgccatg
      961 cgtttggacg tcatcgatgc gccactcgct gccgtttgtg gacctggcga cgacgtagcg
     1021 ttcgatggag cagccacgtg attgttccga tccgaatgcg taaaccgcgc cgccggcaac
     1081 ggcaacgtcg aacaggctga gtcggtcaag gtccagcgat gccgccgacc aggagagtcc
     1141 gtcgggggac gtccaaatcc gtgcctgggt tgcggagtcg caagcgtcgg tcgcgccact
     1201 gccgccgacg gcgacgaagc ctccgtcgaa tgcggttagt gcacgtacac gggcgttgct
     1261 cggcgggaag tcgggaatcc tctgccaacc agtcctcgag ggctccgttg tgggtgctgg
     1321 ttcaagagtc ggagtcggcg tcggctgttg tgttggtgag gtgctcgttt gtggcgctgc
     1381 gctgaggacg ggagcctgcg tgaaggtcgc gaccgggact ggggcgccgg tggtcgcagg
     1441 aggcgcgttg gttgggctgg ccacagccga tgggcttggc gacgtctcgc cgacataggc
     1501 gcagccggtc agggaaatga cgatcaggga cgctaccagg tggtgcagcc gcacgccgct
     1561 aagtcctccg ctatacgcat ctcggacggg gcgatgctgt ttgttgaccg cgatctaaga
     1621 cgtggagcgt gacaccaatg tgtcaccgac gggcatcacg gaacatgtcg aagcgacggc
     1681 ggggacatcg acgcatcggt atgcactaca tatctgggca cgcgggtggc cgctcgatag
     1741 tgcgccgtgc gtcgccctcg ttgcgaccct atggacacgc tcagcactca cgtgactgag
     1801 aatcgcggtt ttgaccgtta gcgaccacgc tggacgagca gagtgaccaa gattgagggt
     1861 ccagcctccc gccagggtcc gctaacgccc gatcatccgc tcaggcaatc atgaaccgca
     1921 tacggccgcg cctggccgct actccgcggg cgttggcacc atgatcaggt cgtgggcggc
     1981 gtatagcgca gcccattcgt cgggtgtcgg ctggccttcg cgcaccatcc gggggctctc
     2041 ttggaaatag gcctcgcgcg gtggggcggg cgagaagtgg ctgtgtgcgc ggcgcgacgt
     2101 tcgtcgcctg actatcgttg atcgaatgcc aaccgtgacg tgggagcagg cgctggcgtg
     2161 gcgcatgaac cgccacctgc ttgaacccgt cggcaacctc ggccctgaag acgtggtcag
     2221 gcgcttgtgc ggcgttcagg cgcaggtctc ttcctcggcc gactttgcca ttcgtgcgcg
     2281 gcaagcgaac tcggaggcag gcgccgtcgc tgaggcgctc gccgacggac ggttggtcaa
     2341 aacctgggcc atgcgcgccg cgctgcacct gctgacgccc gaggacgccg gtgactacat
     2401 ggggctgatg gccgccggac gaccttggca gcggccgtcg tggcagcgct acttcggcct
     2461 cacgccgacg gagatggaca acctccgcga cgccgtaatc gagatcctgg gcaaccgagc
     2521 gatgacccgc gaagagctga ccgctgaagt tacagcgcgc ccgggtttcg cccatatcgg
     2581 ccagggactc aagtcaggct ggggaatgct cttcaagccg atagcgtggc aaggtggcct
     2641 gatccatggt ccaagccaag ggacacgcat cactttcatc cggccgcaag tggcgagcag
     2701 gctctggcgt ccgccgcccg atccggacga ggccggcccg cgtgtgctcg agcgctacct
     2761 ggcggcgtac gggcccgcca cgctcaacaa ctttgccgcc tggctctcgc gcggcacgat
     2821 cgcgccaacg cgtgtcaaga gatgggtctt ggagctgggg gaccgtttga ccgcagtcga
     2881 cgtgagcgga acggcgacgt accttcgatc ggaagacgtg gatgacctgg catcgacaaa
     2941 gtcaagcaac gccgtccggc tcctcggcgg gttcgatacc tgggtccttg gcccgggcac
     3001 cgatgacacg catgtcatcc ctgccgcgcg cagaaacgcg gtcagcaaga cggccggctg
     3061 gatcgcgccg atcgtggtcg tcggtggcgt ggtccgcggg acgtgggaaa tgggcatggg
     3121 caccgtgtcg gtctcctggt ggaaggagtc gggcaagcca ccatcgcggg cgatcgaggt
     3181 cgaagccgcg cgaatcggca gaatccgagg cgagaagctc caggtcgcga tctcgaccga
     3241 ttagtcccga tggggcgggg gcctaaaaca gcagcgtcgg gatggaacca cgaggatggc
     3301 agctggctgt ggcgggggct cgctcacctc gcgggccagc gcgagtaggt cggcggccag
     3361 tcgtcggccg tttccctcgc ggaccgtcgc cgcggacggt gacgagcaac ccctcgccgt
     3421 cgcccgagcg atagatcagc gccctgccgg cagccgccag gtccggtgcc tgtgcctcga
     3481 actcatccca actcgccacg cccccgattg tggcaccagg accagtacca acgtcagcaa
     3541 tgcggacgaa accttttgcc gactgagcct ctcggtactg atgtaggcgc cgaagactcg
     3601 gtgccggccg gttacgactt ggaaagggaa cagggactca atgagggcta tcaaggtatt
     3661 tcacgcacta cgagcagtag tgatccttgg cgcgctggtg ggcgcgctgg cagcgccagg
     3721 tgcggtggga gccgcgagcc gcgactccga tggcgacggc ctacgcaacg cctacgagac
     3781 caactggagc cacacgaacc cgcgcgaccg cgacagcgac aacgacggca agtccgatgc
     3841 tcgcgaaaat cccgatcaag acgggctgtc taaccggcag gagcagcgcc ttgctctcaa
     3901 tccgctcgac tccgattcgg acaacgacaa cgtcgaggat ggcgacgagg atccggacga
     3961 tgacgggctc gataacgagg acgagttcga cgcgcacacg aacccgacgg acgacgactc
     4021 cgacaatgac ggtgtcgagg acgacgatga cgacagcgac tgcgacgagc tgtcgaacga
     4081 caccgagctc ggtgcaggcc tgaagccgtt cgatgccgac tccaacaacg acggcgacga
     4141 agacggcgac gaggacaagg atcacgacgg cgtccagaac gaggacgaag aagacaacgg
     4201 gaccgacatc gacgatgagg actcggacga cgacaacatc gacgatggcg acgatgagga
     4261 cggcgacggg gacggcgacg acgatgacgc agacgacgac aacgacgagg atgtcgagga
     4321 tcccgactgc ggcgacgaag acgaggatga gggcggcgaa gacgaggact aacccgacat
     4381 cgggtcaacc tcacccttag caactcctca gaaactttgc gggcgggccg atctctggac
     4441 ggcccgcccg tccctatgtc ctccgccgct gagcgcccgg atggagatga tggatgcagg
     4501 accattggtg ggtccactgg cgctgatcgg caatggcgga cgagctgttg ggaggggatc
     4561 atggcggccg tgcctgagcc gtcagcatct ggtttacacg accgccgcat cgtcgatgcc
     4621 gtgttggctg gcgatcgtga ttcattccgc gcgctcgtcg agcgggagtc agcgaccgtg
     4681 atcgccgtct gcatgtcagt tctgcgcgac cgcgaagagg cgctcgacgt cgctcaagat
     4741 gcattcgtgc aggcttaccg ggcgctcgcc acgtaccgcg gcgacgggac gttcggcgcg
     4801 tggatagccc gaatcgcctc gcgtctggca gtggctcgcg catccacgac tgcccgccgg
     4861 ccggtgacca ccaccgacga catcaacgca tacgacgttg ccgatgtatc cggcgatggc
     4921 ttcgacctgg agagaaccgt gctgggcgag gagcgtgcgg ccgccctccg tgacgcgatc
     4981 gaacggctgc cggcggatca gcgcgaagtc gtgaacctgc gcttcttccg cgacatgccg
     5041 ctcgagcaga tcgcgctcga aacggcatcg ccactgggca ccgtcaagtc gcgcctgcac
     5101 cgtgcgctgc agcgcctgcg tgagcaggca gatttggggt cagcgtcgtg actgccgcgt
     5161 cgatcgagtt cgacagctgg tttgtcgacg cggttatgga tcgtgtcgcg cgcgagcgcg
     5221 tgcctacccc ggcaaacacg ctccgcgccg ccgtgcgcgg actggacctg agcatggcgc
     5281 tcgcatctgc ttgggcggcc tggcaactgg ctacgacggg gggattcgtt cagcgccgcg
     5341 tccgtctgcg ctccgcgatg ctcctcgcaa ccctgctcct gatcgcggcc atgggcacaa
     5401 cgctggccgc ggccgggacc gcagttgtcg tccaaacggc ggccgaacat ctgagccggg
     5461 taatgattca gacgccgcca ggggagccgt ccctgcccgg cgtggtgctt gtcgagccag
     5521 gtgattcgcc gcagccgatg actactcgcg agcccgcgcc acggaccgcg gatcccgggc
     5581 aagatccaac gcgccagccg ccagttgtcc cgtggcagag ccattcgcca tccccccgta
     5641 cggtcggcca gcccgagagg agtactgcta ctccatcgcg gtccccacaa ccgacggaag
     5701 cagccacgcc gaccgacgcg ccgaacccga ccaggacgcc tcgaccaaca cccagcgcga
     5761 cgccgactga ccgaccgtcg ggaggcgacg atcaggcgcc tcgtgaggat ggcggcgggg
     5821 acgcggacga tggcgccgat ccgcccggcg acgacgggaa cgatggtggc ggtgacgcgg
     5881 acgatggcga cgatccgccc
//
LOCUS       DATLCO010000034         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_37434, whole genome shotgun sequence.
ACCESSION   DATLCO010000034 DATLCO010000000
VERSION     DATLCO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37434"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..946
                     /locus_tag="VMZ33_00965"
     CDS             104..946
                     /locus_tag="VMZ33_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HUR15828.1"
                     /translation="MATRGMGIDIGGTGVKAALVDLETGELVSRKVRMATPKPATPEA
                     VAQTVRTVIDQIGEDNELPPDLPFGIGLPGVVVEGTVMTAANIAKSWIGTNAEELLGG
                     ALGRKVHVLNDADAAAIAEARLGAGRGIAGTVLLLTIGTGIGSGLLVRGRLVPNTEFG
                     HLEYRGREAETRLSGAARLRRGLRWRQWAEEFNGYLERLELYFCPDLIILGGGVSKEL
                     GRFGPMLKSRAPIVAAQFLNTSGIVGAAMYAAGRQESAAPLRLVAVEESDTSDNTVLR
                     TTPA"
     gene            964..1848
                     /locus_tag="VMZ33_00970"
     CDS             964..1848
                     /locus_tag="VMZ33_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HUR15829.1"
                     /translation="MVQVHLLDATYELFRAHFAPRPPRLDPAGRSVTATLGVLENVLY
                     LLRDDGVTHIGCASDHVIESWRNRRWPGYKTGQGVDPVLKSQFTLAEEALGTLGVVVW
                     PMVEWEADDALGTAALRWGNDPAVERVVIMTPDKDMGQCVREDGRVVQFDRRQRVLIN
                     ADSVRAKFGVSPESIPDFLALVGDSADGYPGIPGWGKVSAGAVLTRYGHLEDIPESVV
                     RWDVSVRGGASLAAALREHRELAFLFRELATLTPDVPLPETLDDLRWKGAHRADFTAM
                     CERLGEPELVSRVPRWLD"
     gene            complement(1845..>2297)
                     /locus_tag="VMZ33_00975"
     CDS             complement(1845..>2297)
                     /locus_tag="VMZ33_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUR15830.1"
                     /translation="EYLDWLTGEIKPLIDRTFPTDPSRDATGTIGSSLGGLISLYAMV
                     RHPDVFGMIGAMSSAIRWQRFAILKEIRAFTGERPRIHVDMGGREWKGGLDDARKLRD
                     TLIGSGWLDDHDLNYVEERYAIHREDAWARRLPDALRFLLKETVVPAS"
BASE COUNT          365 a          754 c          786 g          392 t
ORIGIN      
        1 gcgtttgaag tcgccgaact gatcgaacat gtcctacgca gtttagggcc cttcccggaa
       61 gcgtcccgcg ggcctccttc catgagcgcc gtatgcttcg gccgtggcga cacgtggcat
      121 gggcatcgac atcggcggga caggcgttaa ggctgcgctg gtcgatctcg agacgggcga
      181 gcttgtttcg cgcaaggtgc gcatggcgac cccgaagccg gcaactccgg aggctgtcgc
      241 gcagaccgtc cgcacggtga tcgaccagat tggcgaggac aacgagctgc caccggactt
      301 gccgttcggc atcggactgc ccggagtcgt ggtcgaaggc acggtgatga ccgccgcgaa
      361 catcgcaaag tcgtggatcg gcacgaacgc agaggagctc ctcggcggag cgctcggacg
      421 aaaggtccac gtcctgaacg atgccgatgc cgcggcaatc gcggaagcgc gtctcggtgc
      481 cggtcgaggc atcgcgggaa cggtgctgtt gctcaccatc ggaactggga ttggcagtgg
      541 cctgctggtt cgtggccgcc tcgtgccgaa cacggagttc ggccatctgg aataccgcgg
      601 ccgcgaggct gagacgcgac tgagcggtgc ggcccgtctc aggcgagggc tgcgctggcg
      661 ccagtgggcc gaggagttca acggctacct cgagcgtctc gagctctact tctgccctga
      721 cctcatcatt cttggcggcg gcgtcagcaa ggagctgggc cgcttcgggc cgatgctcaa
      781 gagccgcgcg cccatcgtgg cggcgcagtt cctcaacacg tcagggatcg tcggcgcggc
      841 gatgtacgcg gctggccgcc aggaatcggc cgcgccactg cgactcgtcg cggtcgagga
      901 aagcgacacg tccgacaaca ccgttctccg taccacgccg gcctgaggtt cggtccggcg
      961 accgtggtcc aggttcatct cctcgacgcg acctacgagc tgttccgcgc ccattttgcc
     1021 ccgcgcccac cgcgcctgga cccggccggg cgctcggtga ccgcgacgct gggcgtgctg
     1081 gaaaacgtcc tgtacctgct gcgtgacgac ggcgtgacgc acatcggctg cgcctcggac
     1141 cacgtcatcg agtcgtggcg caaccgccgc tggccgggct acaagaccgg ccaaggcgtc
     1201 gatcccgtgc tgaagtctca gttcaccctg gcagaggaag ccctcgggac cctcggcgta
     1261 gtcgtctggc cgatggtcga gtgggaggct gacgacgccc tgggcactgc tgcgctgcgt
     1321 tggggcaacg acccagcggt cgagcgtgta gtgatcatga cgcccgacaa ggacatgggc
     1381 caatgtgtgc gcgaggacgg ccgcgtggtc cagttcgatc gccgacaacg tgtgctcatc
     1441 aacgctgaca gtgtgcgtgc caagttcggc gtctcgcccg agtcgatccc cgacttcctc
     1501 gcactcgtcg gcgactcggc cgacggctac ccgggcatcc cggggtgggg caaggtctca
     1561 gcgggcgccg tgctgacgcg ctacgggcat ctcgaggaca ttcctgagag cgtggtcagg
     1621 tgggacgttt ccgttcgcgg cggggcgtca cttgccgccg cgctgcgcga acatcgggag
     1681 ctggcgttcc ttttccgcga gctggccacg ctgaccccgg atgtgccgct ccctgagacg
     1741 ctggacgatc tccgctggaa aggcgcgcac cgtgccgatt tcaccgccat gtgcgagcgc
     1801 ctgggagagc ctgaactggt gagccgcgtc ccgcgctggc tcgattagct cgccgggacc
     1861 acggtctcct tcaacagaaa gcgcagcgcg tccgggagtc gacgcgccca ggcgtcctca
     1921 cgatggatgg catatcgctc ctcgacgtag ttgaggtcgt ggtcgtcgag ccagccgctc
     1981 ccgatgagcg tgtcgcgcaa cttgcgggcg tcatcgaggc cgcccttcca ctcgcggccg
     2041 cccatgtcga cgtgaatccg tggccgctcg cccgtgaaag cgcgaatctc cttgaggatt
     2101 gcgaagcgct gccagcggat cgccgagctc atcgcgccga tcattccgaa cacgtcgggg
     2161 tggcgcacca tcgcgtacag gctgatcaag cccccgagcg acgagccgat cgtgccggtc
     2221 gcgtcgcgcg agggatcggt cgggaaggtc ctgtcgatga gtggcttgat ctcaccggtc
     2281 agccagtcga gatactc
//
LOCUS       DATLCO010000035        14084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7472684, whole genome shotgun sequence.
ACCESSION   DATLCO010000035 DATLCO010000000
VERSION     DATLCO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14084
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7472684"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..812)
                     /locus_tag="VMZ33_00980"
     CDS             complement(144..812)
                     /locus_tag="VMZ33_00980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUR15831.1"
                     /translation="MRLPDVLGVLGALGVGVVLAAILLASAVSAPPALPTPVPPTIPP
                     LPTPTAAAVATQTPGLEPGETASPGRLIEVGQPAPPLQVTLTDGSLFDTADYAGQPLW
                     INFMATWCPQCIDELPLMETYQLDLEDKLTVVLVDVGEQRSVVRQFMQDVDVDLPVGL
                     DEDSVVQQQWGAYALPVHLFIDGDGVIQDIVFGGAPLEIWDEALSKIVPDLAPIESPA
                     VPEG"
     gene            868..1695
                     /locus_tag="VMZ33_00985"
     CDS             868..1695
                     /locus_tag="VMZ33_00985"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HUR15832.1"
                     /translation="MRSIVSVLDRLVQAVLMAASVTLLTAGLLSFAPSTFGDWQTPEP
                     SVVAGDPLLTVAPGTSPSESPTSPPPGLSFSLAPGATPTQIATPTATGTPEPTPTGGL
                     GPTGTPDYPNRGYATRIRIPSLGIDLPIVSGDLIVPGNRDFYPLCDVAMFMPQFVQPG
                     ESGATYIYAHAQRGMFAPLLRSSQLDNGSGMVGALIEVYTSDNKLHVYELYQVKRHAT
                     DLSLASPAPGEHMLVLQTSEGPSGTVPKLQVAARPLSVVPADLGEANPPANPRVCLP"
     gene            complement(1692..2711)
                     /locus_tag="VMZ33_00990"
     CDS             complement(1692..2711)
                     /locus_tag="VMZ33_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HUR15833.1"
                     /translation="MTSAKPHELESRSEALPSGGGQLWALAWTNESGERTVDEIEEVR
                     AIYGTPDSRLWIDVEDPDKDTIDQLGALLGLHPLVSEDILEQDQRAKIEETPGALHIV
                     MFALTYNGELVPHEIDLVLGERFLLTAHDKDINLRNAPFTRRDPSGHLAAGPDFALWM
                     ITDWLVDGYFPIFDKLGDEIDTLEAEILRRPGAWVVERLFAVRRDLLKVRHAVLPQRE
                     VFNQLTNRDLKLIHPDRIVYFRDVYDHLIRLTDELDSYRELVSTALDIYLSQVNNNLS
                     EVMKRLTSVTAVLAGAGALAGIFGMSEAGLAISFEDMRFWLVTVGIVILSSIGILYFR
                     RIGWI"
     gene            complement(2671..4269)
                     /locus_tag="VMZ33_00995"
     CDS             complement(2671..4269)
                     /locus_tag="VMZ33_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR15834.1"
                     /translation="MSGKGRLLLLAALGAGVFLVGVELMVTAVALPSIVADLADWTRL
                     REASWIVNGYLVAYIAVMPLAGRAADRFNVPALFGGSLVIFAVASVAAGAAPNLETLI
                     GARVVQGLGAGAVVPLATAGASLAFDGAARARAIGVVGALTFLGMAAGPFLGAAVLNN
                     FDIPATNASMAGLVTPSWRWIFYLAVPVALLAVIYTWAAAGSWPAHEARSSLDVVGAL
                     LFTTWVAAGLLMLTTVTELASNTPLLAGAVAAGAVVAWILWTLRTKEPFIDLRLFRDR
                     TFSGAVLLSLLTGYALATAIIGGAVFVDRVRYGLADEQQLVLGSLALSVALGAVGSGF
                     LIRRLGVVLPSLVGLVASIAGLWWLSSANATTPIELFVYGLALFGFGFGLTVTARSAA
                     ALEAVGRRAFGLASGVVTVARMLGMAIGLATLTVLGSNRIEALSVVLVDQAARDTVLP
                     PHLRGRPLENSLVVDALETWAANEAATILSMLFLVAAAVTAVAIVPTLAMRNRPAARG
                     DAGARTEEMATDDLGEASRAGIAI"
     gene            4419..4751
                     /locus_tag="VMZ33_01000"
     CDS             4419..4751
                     /locus_tag="VMZ33_01000"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUR15835.1"
                     /translation="MPSPAHIRSTLATLAAPPEDLRAVASYHKALADPTRLRLLQRLA
                     DAPATVTELIDFVDLSQPLVSWHLRRLRSAGVVETRRAGREVICTLSREALELFRARD
                     RELLGLAS"
     gene            4767..5456
                     /locus_tag="VMZ33_01005"
     CDS             4767..5456
                     /locus_tag="VMZ33_01005"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUR15836.1"
                     /translation="MSSDFRHRVRSSIEGPVAGFFGRLGLTPNALTLIGFGIAIIGAY
                     LAATQIWLLAGVVVAFGAIFDLFDGALARATNKTSKLGAFLDSVFDRAGEVVVYLGVA
                     IGLMAVEHYVGAVLSAGAMAAAMMVSYTRAKSESLGFSSGSGMASVGLAPREVRTVML
                     VGGLIGAGALGGFQQVQCVTAPCPPVVGTGGFVLLGSLALIAIAATLTTLQRIYFVYQ
                     QSKHQPTTDPK"
     gene            5475..6752
                     /locus_tag="VMZ33_01010"
     CDS             5475..6752
                     /locus_tag="VMZ33_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HUR15837.1"
                     /translation="MASNNGKAAASGTANGAKRRKDGKIRVAIVGVGNCASSLVQGRY
                     YYENAKDDEFVPGLMHVNLGGYHIRDIEFVAAFDVDKNKVGKDLSEAIYTKPNNTYVF
                     QQVKNLGVKVERGMTHDGLGKYLSQIIEKAPGPTADIVGILKEREVDVMVSYLPVGSE
                     QATKWYVEQALQAGVGFINAIPVFIGREPYWQRRFAEEGLPVIGDDIKSQVGATITHR
                     VLTRLFMDRGVRLDRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSMLDYELSDD
                     DIHVGPSDHVPWLLDRKWCYIRMEGTTYGDVPLNAELKLEVWDSPNSAGVITDAIRCL
                     ALGLDRGLKGTLVAPSSYFMKSPPHQIHDDIAFNRVEAFIRGEDNETLVGTEAKQAKK
                     TRRLAKGHNSEPQTKHEAAPAVKSDPVGVAAAD"
     gene            6768..7784
                     /locus_tag="VMZ33_01015"
     CDS             6768..7784
                     /locus_tag="VMZ33_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUR15838.1"
                     /translation="MTDQRSGVKYAPHRREHSSTRVGRVVRRARERSAVHGYRAAAAA
                     ISRVPVRVSWPLGRAVFLGGYLAWPKKRRIILSNASHVLGLPASDRRVRHLARRIYQT
                     YARFVIELMRLPALPADEPMRLVSAGSGPGSGQSFIELFEKLRAEGRGLIAVSAHIGS
                     IDLLAGAFALRGLPTYGLADDNAYPELFEEMNAQRRRWGLEVIPWRNMRRVFTALRQP
                     AILGLVVDWGYRDDGIPVKLFGEWTTLPAGPAMLAAKTKAAIVPVVCRRLRDGTYEAR
                     HYDAIEVADTSATEMQRATQLIATAIEDMVATAPEQWYSFKPIWPQTEAEKDALAARV
                     GPSA"
     gene            7790..8776
                     /locus_tag="VMZ33_01020"
     CDS             7790..8776
                     /locus_tag="VMZ33_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUR15839.1"
                     /translation="MAEERYVRRARPTKRPQSRLGSVRSLLAARALIVLAEVAQRLPP
                     ALLQRLGHLVGGVMYRVRPARRRLVRANLERIVGFLAAREMGGERVAAAARDGRALDR
                     LVRDAFGHYIRGYLELAALPAYARSDRLARVKPDDPQLLDRAFAPGSTVICGLHFGAL
                     EIPGLWATHFLGRRITAPMETIDDPRLQSYFEGTRKRTGLNVIPMERAATELRAALAR
                     GEAVALVADRPVGGAGAQVELFGAPARLPLGPAALALESGAPVWMIATRRSGWTDYRS
                     RIEKIEMPTTGIPRAQLRSFLHNQAAAFERAVADAPDQWWTTFFQIWPDIPA"
     gene            8773..9597
                     /locus_tag="VMZ33_01025"
     CDS             8773..9597
                     /locus_tag="VMZ33_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HUR15840.1"
                     /translation="MTTEATTERRGRADLHIHTLASDGVSSVAQVIDIAIARGLDVIA
                     ITDHERVDSALAAKAMVEARGLPIEIVVGEEVSTRGGHVIGLFINERIRPWGPLRSTI
                     AQIHEQGGLAIVAHPLVPYPLTCASGRAVLALLNDPDPAFHPDGVEAFNPTTAGSRWG
                     QRAAAFVAGLPVAAVASSDAHCAEDVGQAFTTFEGRTAADLRAAIENRSTQWEGTKYS
                     LPGQLSTFRRQLAKYSVGVRDNVRGKVRRDGTGRDLGYPGGRMRPPRLDLDEAEKR"
     gene            9594..10859
                     /locus_tag="VMZ33_01030"
     CDS             9594..10859
                     /locus_tag="VMZ33_01030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUR15841.1"
                     /translation="MKIGLVTPYIYPLPGGVNAHVRYLYENLLAHGHDVRIISSVHGP
                     QRQSEGDIIRLGYGFSVPANGSVGTLTFSPRLRAAVKEMLDQEKFDVLHFHEPFVPFL
                     SLLLLRESRSINVATFHAFAGWSPAYEFGRRALGRFAKRLHGRIAVSAVARHFIDRYF
                     PGDYKIIPNGVDISRIEGVQPIARYKDGVLNIFFLGRFESRKGAMYLLKAYRQLRKRG
                     IECRLLLAGTGPQEREIRRYIATRRLGDVELLGRISDDDKARYFATADVYVSPATGQE
                     SFGIVLLEAMAAGKPIVCSDIHGYKGVVRRGEQALLVPPKDVKALTEALDTLLRDPEM
                     RARMGASGRERVVQFGWGNITNKVEEYYRFVIRRTVLQGAAPAHLDPGLVRAAREGQP
                     LIGVGRRNVVHPAGPAPVNPSSLPPAPGS"
     gene            complement(10831..>14084)
                     /locus_tag="VMZ33_01035"
     CDS             complement(10831..>14084)
                     /locus_tag="VMZ33_01035"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HUR15842.1"
                     /translation="DDNPGERDGDRLYVAGDNEVGVFELSNKQQIATIPGTYEAIAVD
                     EEGHWLLLATADGQISRIDTAALDELRIHPDLVPDLTPEPLTELLGATAPITELAVVF
                     EEIVAIAEDGTVFSADRSDGTGLAQVTYPVPLDPIEVDVHSSVIVDPAEVGDPAITAS
                     AIAAVTAGNADVLRSAIERATEPVPITGLLSKLEVENLQEKIDGGELPGVTIEDGDGL
                     AIGLETGIVILDPRTLNELAFYGTFAPVTGMDLVAQGRDEPTIYAANGTELARLLMPR
                     DDAARLGSAVPMPNTVTDVQWNPATTMVHILGRTQDDSASTVYVVEPRSDSVFADAAL
                     TSEPVALLMDADKQRPAEDRDDILTLDGAGNMQVVETGNNQFAYRFPGVVLGSLMAVC
                     IYLLARFLFARRSVAIIASLLVLADGMFFANARIAMNDTYVAFFIVAAFTLFVPLWLG
                     RWRNPIVIALAFVAVGVLLGLAFSSKWVGAYAIGGMGLLILLRSALGRIVTLVAMVGL
                     TALLGYIAITPNSTVVNPQLNYPFLGLMLALTALLAVGMTLRPVRFTRDELRLAVIGP
                     LLAGGAVAAYGAYRLVAGPPPAISASLTPQRMVIYGVAVAAIGIIALGLAWLLGRYGY
                     GPLVDAATVNPDREPSSPPPSRGWLRPGSGLLGLPWLLALGAITVIPVAVYVAHYIPW
                     IDMGNYWLTVPQGESGQNLIDLQKSMYDYHNFLRATHPASSPWWAWPLDLKPVWFEQN
                     DYANSTTAVIYDTGNLVLFWLAIPAVAWLCWQAWKRRSLSLTFVAIAIASLWLPWARI
                     DRATFQYHIFTTLPFSFLALAYFLAELWHGPSRRTWQLARVAAALAVIGAPLLWLLRL
                     PLCGIARTEQVNEGTEVCASLSRVLVLTDVQMIGVLLAVGGLITAGVLFWRFRAAGGP
                     VGNRALVLPIAFSVALVGLAITVVGAGLQDQAREVFSANVQAEWPALGALLLLLVPAY
                     FVLRATDPRKFTLGVIGAAAVWFVAFYPNFAALPVPTAFSQIHLGLLPTWNWGFQFGV
                     NMDEPNRGSPEMGAVALLAIAVVVLCLTSIYASRAWRARDDEPVVNSPVPEAG"
BASE COUNT         2485 a         4745 c         4659 g         2195 t
ORIGIN      
        1 ctcggccccc cacgggtcgt cgacgatacg gctgagggtg ccgtcgaaat cgctgaccac
       61 gagaagcggc cgtcgcgaaa ggtgcgctgc cgctacctca acggccgcgc ggaccgtgac
      121 gggcgtgtgg ccgcgctcga ctgtcaaccc tccgggacgg cgggcgactc gattggcgcc
      181 aaatcgggca cgatcttcga cagtgcctcg tcccagatct ccagcggcgc cccaccgaag
      241 acgatgtcct gaatcacgcc gtcgccgtcg atgaagaggt gtaccggcag tgcgtaggcg
      301 ccccactgct gctgcaccac cgagtcctcg tcgaggccca cgggcagatc cacgtccacg
      361 tcctgcatga actggcgcac aaccgaacgc tgctcgccga catcgaccag cacgacggtg
      421 agcttgtcct ccaggtcgag ctgataggtc tccatgagcg gcagctcgtc gatgcactgc
      481 gggcaccagg tcgccatgaa gttgatccac aacggctggc cggcgtagtc agcggtgtcg
      541 aacagcgagc catccgtcag cgtgacctgg aggggcggtg ccggctggcc gacctcgatg
      601 agtcgaccgg gagacgccgt ctcacccggc tcgaggcccg gtgtctgcgt ggcgacggcc
      661 gccgctgtag gagtcggcag gggcgggatc gtaggtggca cgggagttgg cagcgctggg
      721 ggcgcggaga ccgcgctcgc gagaaggatc gccgccagga caactccgac gcccaacgcg
      781 cccagcaccc cgagaacgtc gggtaggcgc attcgcgcgg aatgtactac ggcggtaaaa
      841 agtgggctgg gttagcatcg gccgcccttg agatccatcg tttcagtact cgatcggctc
      901 gtgcaggccg tgctcatggc agcgagcgtc acgctcctga cggcagggct gctgtcgttc
      961 gccccctcca cgttcggtga ctggcagacg ccggagccca gcgtcgttgc gggcgacccg
     1021 ctgctgaccg tcgcgccggg cacgtcaccc agcgaatcgc caacttcgcc gccgcccggc
     1081 ctgagcttca gcctggcgcc gggcgcaaca ccgacccaaa tcgcgacgcc gacggcgacg
     1141 gggacgcccg agccgacacc gaccggcggg ctcgggccca caggcacacc cgactatccg
     1201 aaccgcggat atgccacgcg gatccgcatc ccgtcgctcg gcatcgatct gccgatcgtg
     1261 tcgggcgatc tcatcgtccc cgggaaccgc gacttctacc cgctgtgcga cgtggccatg
     1321 ttcatgcccc agttcgtcca gccgggcgag agcggcgcga cctacatcta cgcccacgct
     1381 cagcgcggaa tgttcgcccc gctcctgcgg tcatcacagc tcgacaacgg gtccgggatg
     1441 gtgggtgcgt tgatcgaggt ctatacctcc gacaacaagc tccacgtgta cgagctctat
     1501 caggtcaagc gccacgcgac cgatctctcg ctggcgagcc cggctccggg cgagcacatg
     1561 ctcgttttgc agacgtcgga gggtccatcg ggcacggtcc ccaagcttca ggtcgccgcg
     1621 cggccgctga gcgttgtgcc agccgatctg ggcgaggcca atcccccggc aaacccgcga
     1681 gtctgcctgc cttagatcca gccgatccgg cgaaagtaga ggatcccgat cgaagacagg
     1741 atcacgatcc cgaccgtcac cagccagaac cgcatgtcct cgaagctgat ggccaggccg
     1801 gcctcgctca tcccgaagat cccggcgagg gcaccggccc cggccaggac cgccgtcact
     1861 gacgtgagcc tcttcatgac ctccgacagg ttgttgttga cctgtgagag gtagatgtcc
     1921 aaggctgtgc tgaccagctc gcggtagcta tcgagctcgt cggtcaggcg gatcaggtgg
     1981 tcgtagacgt cccggaaata cacgattcga tcggggtgga tcagcttcag gtcacgattc
     2041 gtgagctggt tgaacacctc gcgctgcggc aggacggcgt ggcgcacctt gagcaggtcg
     2101 cgtcgcacgg cgaacagccg ctccacgacc caggcgcccg gtcgacggag gatctcggcc
     2161 tccagcgtat cgatctcatc gcccagcttg tcgaagatcg ggaagtagcc gtcgaccagc
     2221 cagtccgtga tcatccacag cgcaaagtcc gggcccgcgg cgaggtgacc gcttggatcc
     2281 cggcgcgtga agggtgcgtt gcgaaggttg atgtccttgt cgtgggcagt cagcaggaag
     2341 cgctcaccga gcacgaggtc gatctcgtgt ggaacgagct cgccgttgta cgtgagcgca
     2401 aacatgacga tgtgcaacgc gccgggcgtc tcttcgatct tggcgcgctg atcctgctcg
     2461 aggatgtcct cgctcaccag cgggtgcagc ccgaggaggg cccctagctg atcgatcgtg
     2521 tccttgtcgg ggtcctcgac gtcgatccac aggcgcgaat cgggcgttcc gtagatcgcc
     2581 cggacctcct cgatctcgtc aacggtgcgt tcgccggact cattcgtcca ggcgagtgcc
     2641 cacagctgac cgccgccgga gggcaacgcc tcagatcgcg attccagctc gtgaggcttc
     2701 gccgaggtca tctgttgcca tctcctccgt ccgcgcaccc gcatcgccgc gtgcggcggg
     2761 ccgattgcgc atcgcgagcg taggcacaat cgcgaccgcc gtcacggccg ctgcgacaag
     2821 gaacagcatc gagaggatgg ttgcggcctc gttggcagcc caagtctcca gcgcgtccac
     2881 gacgagcgag ttctccagcg gccgaccacg gaggtgtggc ggcaggacgg tatcgcgtgc
     2941 cgcctggtca acgagaacga cgctgagcgc ctcgattctg ttcgagccga gcaccgtgag
     3001 cgtggcgagg ccgatggcca tgcccaacat ccgcgcaacg gtcacgaccc ccgaggccag
     3061 tccgaaagca cgccggccga cggcctccag ggcggcagcc gagcgggcag tcacggtcag
     3121 gccaaatccg aaaccgaaca gggccaggcc gtaaacgaaa agctcgatcg gcgtggtcgc
     3181 gttggcgctg gacagccacc agaggccggc gatgctggcg acgaggccga caaggctggg
     3241 cagaacgacc cccagacggc ggatcaggaa gccggatccg acagcgccga gcgcgaccga
     3301 cagagccagg ctgccgagca cgagctgctg ctcgtcggcg agaccgtatc ggacccgatc
     3361 cacgaacaca gcgccgccga tgattgccgt cgccagcgca tagcccgtca acaggctgag
     3421 cagcacggca ccggaaaagg tgcggtcgcg gaacaggcgc agatcgatga agggctcctt
     3481 cgtgcgcagg gtccagagga tccacgcgac gacagcaccc gcggcgacgg cccccgccag
     3541 gagcggtgtg ttggaggcaa gctccgtgac ggtcgtgagc atcagcaagc cggcggcgac
     3601 ccacgtcgtg aacagcagcg cgcccacgac gtcgaggctg ctgcgagcct cgtgagccgg
     3661 ccagctcccg gcggctgccc acgtgtagat caccgccagc agggccaccg gcacggcgag
     3721 gtagaagatc cagcgccagg atggtgtgac gaggcccgcc attgaagcgt tcgttgccgg
     3781 gatgtcgaag ttgttcaaga cagccgcgcc gagaaatggc ccggcggcca tgcccaggaa
     3841 cgtcagcgcg cccacgacgc cgatggcccg ggcgcgggcg gcaccatcaa aggcaagact
     3901 tgcgccggcc gtggccagtg gcacgaccgc gcccgcgccc agcccctgga cgacgcgagc
     3961 gccgatcaac gtctcgaggt tgggcgctgc gccggccgcg acgctcgcta cggcgaagat
     4021 cacgaggctg ccaccgaaca gagctggcac gttgaagcga tcagcggccc ggccggcgag
     4081 gggcatcacc gcgatgtacg ccacgaggta gccgttcacg atccacgacg cctcccggag
     4141 gcgagtccag tcggcgagat cggcaacgat gctgggcaac gccacggccg tcaccatcaa
     4201 ctcgacgccg acgaggaaca cgccagcgcc gagcgccgcg agcaacagaa ggcggccctt
     4261 acccgacatt gcgccgattg tcgtctagtg ggggtgcggc aggagctgac cccttgacgc
     4321 gtcgtataaa gcacccttaa tatgagcagg catttatgta tcggcgccgc gtctcagcga
     4381 acgcgccggc cctgccgggc taaagatcaa ggaagtacgt gccctctccc gctcacatcc
     4441 gctcgactct ggccacgctc gcggccccgc ctgaggacct gcgcgcggta gcgtcgtacc
     4501 acaaggcact cgccgatccg acccgcctgc gcctcctgca gcgcctggcc gacgcgccgg
     4561 caaccgtcac ggagctcatc gacttcgttg acctttcgca gcccctcgtc tcgtggcatc
     4621 tgcgccgcct ccggtcggct ggtgtcgtcg agacccgacg tgccggccgt gaggtcatct
     4681 gcaccctcag ccgcgaggct cttgagttgt ttcgggcacg tgatcgcgag cttctcggac
     4741 tggcgagcta gcgcgaaatg agcgtcgtgt cgtccgattt ccgccaccgc gtgcgctcga
     4801 gcatcgaggg cccggttgcg ggcttcttcg gtcgtctcgg gctcacgccc aacgccctca
     4861 ccctgatcgg cttcggcatc gccatcatcg gcgcctacct cgccgccact cagatctggc
     4921 tccttgccgg cgtcgtggtc gccttcggag caatcttcga cctcttcgac ggtgctctgg
     4981 cgcgggcgac caacaagacc agcaagctcg gcgcgttcct ggacagtgtc ttcgaccgcg
     5041 ccggcgaggt agtcgtgtac ctcggcgtcg ccatcgggct catggccgtg gagcactacg
     5101 tcggtgcggt tctatcggcg ggcgccatgg ccgccgccat gatggtcagc tacacgcgtg
     5161 ccaaatcgga gagcctcggc ttcagctcgg ggagcggcat ggcaagtgtc gggctcgccc
     5221 cacgagaggt ccgcaccgtg atgctcgtcg gtggcctcat cggcgccggc gcgctgggcg
     5281 gctttcagca ggttcaatgc gtcaccgcgc cgtgtccgcc ggtcgtcggc acgggcggct
     5341 tcgtgctcct gggcagcctc gccctcatcg caatcgcggc gacactcacc acgcttcaac
     5401 gcatctactt cgtctaccag caatccaagc accagcccac gacagatccc aagtagggaa
     5461 ggagaaaacc aacgatggca agcaataacg gcaaggcagc agcgtcggga accgccaacg
     5521 gcgcgaagcg ccggaaagac ggcaagatcc gtgtcgccat cgtcggcgtc ggcaattgcg
     5581 cgagcagcct ggtccagggc cgctattact acgagaacgc caaggacgac gaattcgtgc
     5641 ccgggctgat gcacgtcaac ctgggtggct accacatccg cgacatcgag ttcgtggccg
     5701 ccttcgacgt cgacaagaac aaggtcggca aggatctgtc cgaggcgatc tataccaagc
     5761 ccaacaacac gtacgtcttc cagcaggtca agaacctggg cgtaaaggtc gagcgcggaa
     5821 tgacccacga cggcctgggc aagtacctct cacagatcat cgagaaggcg cccggcccca
     5881 cggctgacat cgttggcatc ctcaaggagc gcgaagtcga cgtcatggtc tcgtacctgc
     5941 ctgtcggcag cgagcaggcc accaaatggt acgtcgagca ggctctccag gcaggcgtgg
     6001 gcttcatcaa cgccattccc gtgttcatcg gtcgtgagcc ctactggcag cgccgtttcg
     6061 ctgaggaagg cctgccggtc atcggcgacg acatcaagag ccaggtcggc gccacgatca
     6121 cgcaccgggt gctgacacga ctgttcatgg accgtggcgt acggctggac cgcacgtacc
     6181 agctgaactt cggtggcaat accgacttcc tgaatatgct cgagcgcgag cgccttgagt
     6241 ccaagaagat cagcaagacc aatgccgtca cgagcatgct cgattacgag ctgtcggacg
     6301 acgacatcca cgtcggcccg tcggaccacg tgccgtggct gcttgaccgc aagtggtgct
     6361 acatccgcat ggagggcacg acctacggtg acgtgccgct caacgccgag ctcaagcttg
     6421 aggtctggga ctcacccaac agcgccggcg tcattaccga tgccatccgc tgcctggcgc
     6481 tgggactcga tcgcggcctg aagggcacgc ttgtcgcacc gagctcctac ttcatgaaga
     6541 gcccgccgca ccagatccac gacgacatcg ccttcaaccg cgtcgaggcg ttcattcgtg
     6601 gcgaggacaa tgagactctc gtgggcactg aggcaaagca ggcgaagaag actcgccggc
     6661 tggccaaggg ccacaacagc gagccgcaga ccaagcacga ggctgcccca gccgtgaaga
     6721 gcgaccccgt cggagtcgcg gcagccgact gagctagtcg gaagctaatg accgatcagc
     6781 ggagcggggt gaagtacgcc ccacaccgcc gtgaacactc gagcacccgc gtggggcggg
     6841 ttgtccgccg ggcgcgcgag cgctcggcgg ttcacggtta ccgggccgcc gccgccgcaa
     6901 tctcgcgcgt ccctgtgcgc gtgagctggc cccttggccg cgcggtgttt ctgggtggct
     6961 atctcgcctg gcccaagaaa cgacggatca tcctgtcgaa cgcatcgcac gtgctcgggt
     7021 tgcccgcgag tgaccgccgt gttcggcacc tggcaaggcg catttaccag acctacgcgc
     7081 ggttcgtcat tgaactgatg cgtctgccgg ccttgccggc cgatgagccg atgagactcg
     7141 tcagcgccgg cagtggcccg gggagtggcc agtcgttcat cgagctcttc gagaaactcc
     7201 gcgccgaagg ccgtggcctg atcgcggttt cggcgcacat tggcagcatc gacctgctgg
     7261 ccggcgcctt tgcgctgcgt ggcctcccga cgtacggtct ggctgacgac aacgcctacc
     7321 ccgagctgtt cgaggagatg aacgcgcagc gacgacgctg ggggctggag gtcatcccgt
     7381 ggcgcaatat gcggcgtgtc ttcaccgcgc tacgtcagcc ggcgatcctc ggcctcgtcg
     7441 tggattgggg ttatcgcgac gacggcatcc cggtgaagct gttcggtgaa tggacgacac
     7501 tccccgccgg cccggccatg ctcgccgcca agacgaaggc cgcgatcgtg cccgtggtgt
     7561 gtcgccggct ccgtgacggc acctacgagg cccggcacta cgatgccatt gaggtcgccg
     7621 acacctccgc cacggagatg cagcgggcca ctcagctcat cgccacggcg atcgaggaca
     7681 tggtcgcgac cgcgccggag cagtggtaca gcttcaagcc gatctggccg cagaccgagg
     7741 ccgagaagga cgcactcgct gcgcgcgttg gcccgagcgc atgaccgccg tggctgagga
     7801 gcgctacgtt cgccgcgcgc gtccgacgaa gaggccgcaa tcccggttgg gttcggtgcg
     7861 ttcgttgctt gcggcacgtg ccttgatcgt gcttgctgag gtcgcccagc gcctcccgcc
     7921 ggcgctcctc caacgcctcg gccacctcgt tggcggagtc atgtaccggg tccggccggc
     7981 acggcggagg ctcgtgcgcg ccaacctgga gcgcatcgtc ggcttcctgg ccgcgcgcga
     8041 gatgggtggc gagcgagttg ccgcagccgc gcgcgacggg agagcgctcg atcggctcgt
     8101 ccgcgacgcc tttggccact acattcgggg ctacctggaa ctggcggcgt tgccagccta
     8161 cgcacgatcg gatcgcctcg cacgggtgaa gccggacgat ccgcagctac ttgaccgggc
     8221 gtttgccccc ggctcgaccg tcatctgcgg gctccacttc ggcgcactcg agattcccgg
     8281 cctgtgggcc actcatttcc tcgggcgcag gatcacggcg ccgatggaaa cgatcgacga
     8341 cccccgcctt cagtcgtact tcgaggggac gcggaagcga acggggctga acgtgatccc
     8401 catggaaagg gccgcaaccg agcttcgcgc cgcgctcgct cggggcgagg ccgtcgcgct
     8461 cgtggctgat cggcctgtag gaggcgcagg cgcccaggtc gagctttttg gcgctcctgc
     8521 ccgcttgcca ctcggacccg cggcgctggc gctggagagt ggggcgccgg tgtggatgat
     8581 cgcgactcgg cggagcggct ggaccgacta ccgatcgcgc atcgagaaga tcgagatgcc
     8641 gaccacgggc atcccgcgcg cccagctgcg ttcgttcctg cacaaccagg cagccgcatt
     8701 cgagcgggcc gttgccgatg cgcccgatca atggtggaca accttcttcc agatttggcc
     8761 cgacatcccg gcatgacgac cgaggcaacg accgaacggc gcgggcgggc cgacctgcac
     8821 atccacactc tcgcgtctga cggtgtatcg agcgtcgccc aggtgatcga tatcgccatc
     8881 gcccgaggcc tggacgtgat cgcgatcact gatcacgagc gtgtcgactc ggcgcttgct
     8941 gcgaaggcga tggtcgaagc ccgcggcctg cccattgaaa tcgtcgtcgg cgaggaagtg
     9001 tcgacgcgtg gcggccacgt gatcgggctt ttcatcaacg agcggatccg gccgtggggc
     9061 ccactgcgtt cgaccatcgc ccagattcac gagcagggtg gcctggcaat cgtggcccat
     9121 cccctcgtcc cctatccgct gacctgcgcc agcggccgtg ccgtgctggc actcctcaac
     9181 gaccccgacc cggcgttcca ccccgacggc gtcgaggcgt tcaaccccac gaccgccggc
     9241 agtcgctggg gtcagcgcgc ggcagcgttc gtggccggac tcccggtcgc ggcggtggcc
     9301 tcgagcgacg cgcactgcgc cgaggacgtc ggccaggcct ttacgacctt tgaaggccgc
     9361 acagctgccg atctgcgagc cgctattgag aaccggtcga cccaatggga aggcacgaag
     9421 tattcgttgc cagggcagct ctcaaccttt cgccggcagc tggccaagta cagcgtcggt
     9481 gtccgcgaca acgtccgcgg caaagtgcga cgcgatggca ccgggcgcga cctgggctat
     9541 cccggaggcc gcatgcgccc tccgcgcctc gaccttgacg aagcggaaaa aagatgaaga
     9601 tcggcctcgt cacgccgtac atctatccgt tgccgggcgg cgtcaacgcc cacgttcgct
     9661 acctgtacga gaacctcctc gcccacggcc acgacgtgcg gatcatcagc agcgttcatg
     9721 ggccacagcg ccagagcgaa ggcgacatca ttcgcctcgg atacggcttc tcggttcccg
     9781 ccaacggctc ggtcgggacc ctgacgtttt caccgcgcct gcgagcggcg gtgaaggaga
     9841 tgctcgatca ggagaagttc gacgttctcc acttccatga gccgttcgtg ccattcctgt
     9901 ccctgctgct cctgcgcgag tcgcggagta tcaacgtagc cacgttccac gcgttcgccg
     9961 gttggagtcc cgcctacgag ttcggtaggc gcgctctggg ccgttttgcc aaacgcctgc
    10021 atggccggat cgccgtcagc gccgtggcgc gccatttcat cgaccgctac ttcccgggcg
    10081 actacaagat catccccaac ggcgtcgaca tcagtcgtat tgaaggcgtg caacccatcg
    10141 cgcgctacaa ggacggcgtc ctgaacatct tcttcctcgg ccgctttgaa agccgcaagg
    10201 gcgcgatgta cctgctcaag gcgtatcggc agctccggaa gcgcggcatc gaatgccggc
    10261 tgctgctcgc cggcaccgga ccccaggagc gcgagatccg tcgctacatc gccacgcgcc
    10321 ggctgggcga cgtcgaactg ctgggtcgaa tcagcgacga cgacaaggcc cgctacttcg
    10381 ctacggcgga cgtgtacgtt tcccccgcga cgggccagga gagcttcggg atcgtgctac
    10441 tcgaggccat ggcagcaggc aagccaatcg tctgttccga tatccatggc tacaagggcg
    10501 tcgttcggcg cggcgagcag gcgcttctcg tgccgccgaa ggacgtcaag gcgctgactg
    10561 aggcactcga caccttgctg cgcgacccag agatgcgcgc ccgaatgggc gcttcaggcc
    10621 gcgaacgcgt cgtccagttc ggctggggaa acatcactaa caaggttgag gagtactacc
    10681 gcttcgtgat ccgccgaacc gtgctgcaag gcgccgcgcc ggcacacctc gatcccggcc
    10741 ttgttcgtgc cgcgcgcgag ggtcagccgc tcatcggcgt tggccgccgc aacgtggtcc
    10801 acccagccgg cccggccccg gtcaacccgt ctagcctgcc tccggcaccg gggagttgac
    10861 gaccggctcg tcgtcacggg cgcgccacgc acgcgaggcg tagatcgatg tgaggcacag
    10921 gacgactact gcgatagcca gcaaggcgac ggcgcccatt tccggcgacc cgcgattggg
    10981 ctcgtccatg ttcacgccga actggaagcc ccagttccat gtcggaagca ggccgaggtg
    11041 gatctgcgag aaggcagtgg gcacggggag tgccgcgaag ttcgggtaga aggccacgaa
    11101 ccacaccgcc gccgcaccga tgacgccgag ggtgaacttg cgcgggtccg tggcgcgcaa
    11161 cacgaaatag gcaggcacca ggagcagaag cagcgcgccg agcgctggcc actccgcctg
    11221 cacgttggcg ctgaatactt cgcgtgcctg atcctggaga ccggcgccga caacggtgat
    11281 cgcgagcccc acgagagcca ctgagaaggc gatcgggagg acgagggccc ggttgccgac
    11341 cggtccgccg gcagcacgga agcgccagaa cagaacgccg gccgtgatca ggccgcccac
    11401 agccagcagg acgccgatca tctgcacgtc ggtcaggacg agtacgcgtg acaggctggc
    11461 acacacctcc gtgccctcgt tgacctgctc cgtccgcgcg atgccacaca acggcaggcg
    11521 cagcagccac agcagcgggg cgccgatgac cgcgagcgcg gccgcgacgc gcgccagctg
    11581 ccacgtccgt cgcgacggcc cgtgccagag ctcggccagg aagtacgcga gtgccaggaa
    11641 cgagaacggc agggtcgtga agatgtggta ctggaaggta gcgcggtcga tgcgtgccca
    11701 cggcagccac agggacgcga tggcaatcgc gacgaatgtc aggctgaggc tgcgccgctt
    11761 ccaggcctgc cagcacagcc acgctacagc ggggatggcg agccagaaca ggacgaggtt
    11821 gccggtgtcg tagatgaccg cggtggtgga gttggcgtag tcgttctgct cgaaccagac
    11881 cggcttgagg tcgagcggcc aggcccacca cggcgaggac gccgggtggg ttgcacgcag
    11941 gaagttgtgg tagtcgtaca tcgacttctg caggtcgatc aggttctggc cgctctcgcc
    12001 ctggggcacg gtcagccagt agttgcccat gtcgatccac gggatgtagt gggcgacgta
    12061 aaccgccacc gggatgacgg tgatggcgcc gagggcgaga agccacggca agccgagcag
    12121 tcccgagccg ggccgcagcc agccgcgcga cggcggcggg gacgacggct cgcggtccgg
    12181 gttgaccgtc gcagcgtcga ccagcgggcc atagccgtag cggccgagaa gccaggccag
    12241 ccccagcgca ataattccga tcgcggccac ggccacgccg tagatgacca tccgctgtgg
    12301 tgtgagcgac gcgctgatgg ccggtggcgg ccccgcgacc agccgatatg cgccatatgc
    12361 cgccacggcc ccaccggcca ggagcgggcc aatgaccgcc aggcgcagct catcgcgcgt
    12421 gaatcggacc ggccggagcg tcatccccac agcgagcagc gccgtgagcg ccagcatcag
    12481 gcccaggaac gggtagttga gctgcggatt gaccacggtc gagttcggag tgatcgcgat
    12541 gtagcccagg agcgccgtca gaccaaccat cgcgacgagc gtgacgatcc gaccgagagc
    12601 gctgcgcagc aggatgagca ggcccatgcc accgatcgcg taggcgccga cccacttcga
    12661 cgagaaggca aggccgagca ggactccgac tgccacgaaa gcgagcgcga tgacgatggg
    12721 attgcgccac cgtccgagcc ataggggcac gaacagggtg aaggccgcca cgatgaagaa
    12781 cgccacgtac gtgtcgttca tggcgatgcg cgcattggcg aagaacatgc cgtcggccag
    12841 cacgagcagc gacgcgatga tcgcgacgct tcggcgagcg aacaggaacc gcgccaggag
    12901 gtagatgcag acggccatga gcgagcccag gacgacgccg ggaaaccggt acgcgaactg
    12961 gttgttaccc gtctcgacga cctgcatgtt cccggcgccg tcgagggtca ggatgtcatc
    13021 gcgatcctcc gccgggcgct gcttatcggc atccatgagc agtgccactg gttcgctcgt
    13081 gagcgccgcg tcggcgaaga cggagtccga ccgcggctca acgacataga ccgtcgacgc
    13141 gctgtcgtcc tgggtgcggc ccaggatgtg gaccatcgtc gtcgccgggt tccactgcac
    13201 gtcggtgacg gtgttcggca tcggcacggc cgagccgagg cgcgccgcgt cgtcgcgggg
    13261 catgagcagc ctcgccaatt cggtgccgtt cgcggcgtag atcgtcggtt cgtcacgacc
    13321 ttgcgcgacg aggtccattc cggtaaccgg cgcgaaggtg ccgtagaagg ccagctcgtt
    13381 gagcgtgcgc ggatcgagga tcacgatgcc cgtttcgagc ccaatggcca ggccatcgcc
    13441 gtcctcgatc gtgacgccgg gcagctcgcc gccgtcgatc ttctcctgca ggttttcaac
    13501 ctcgagcttg gacagcagcc cggtgatcgg cacgggctcc gttgcgcgct cgatggccga
    13561 gcgcagcacg tcggcgttgc ccgccgttac tgctgcaatc gcggatgccg ttatagccgg
    13621 atcgcccacc tcggctgggt cgacgatcac gctcgagtgg acgtcgacct cgatcggatc
    13681 cagcggcacg ggatacgtga cctgcgcgag accggtgccg tcgctgcggt cggcggagaa
    13741 gaccgtgccg tcctctgcga ttgcgacgat ctcctcgaat acgacggcca gctcggtgat
    13801 gggcgccgtt gctcccaata gctccgtgag tggctccggc gtcaggtcag gaaccaggtc
    13861 cgggtgaatt cgcagctcat cgagggctgc ggtgtcgatg cgcgagatct gcccatcggc
    13921 cgtggcgagc aggagccagt ggccttcctc atcgacggcg atcgcctcgt acgtgcccgg
    13981 aatggtggcg atctgctgtt tgttgctcag ctcgaagacg cccacctcgt tgtcgcccgc
    14041 tacgtagagc ctgtcgccgt cgcgctcacc ggggttgtcg tctg
//
LOCUS       DATLCO010000036         4718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6728158, whole genome shotgun sequence.
ACCESSION   DATLCO010000036 DATLCO010000000
VERSION     DATLCO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6728158"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VMZ33_01040"
     CDS             complement(<1..755)
                     /locus_tag="VMZ33_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUR15843.1"
                     /translation="MTDDLPAWSEPFVPEARERLIRAAQHGLMDREWAWGGATGAGVK
                     VAIIDSGVEGTHPVVQGRLVESVAVVIIDDEAQVVPDEPVDMFGHATACAGIVVGLAP
                     EVEIYSVRVLGADLRGKGAAFLAGLEWAVQKGVHTMNLSLSSKSERLFPFFHEIVDDA
                     YFKQVSLVCAVNNVPGLSFPSTFSSVFSVAAHAIPDPYAFFYNPTPPVEWGAWGVDVP
                     IGWKDGGTTVATGNSFAAPHMCGLVARILSKHPG"
     gene            complement(781..1554)
                     /locus_tag="VMZ33_01045"
     CDS             complement(781..1554)
                     /locus_tag="VMZ33_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUR15844.1"
                     /translation="MSDPGLPAALEALRLAARRSSVARRLDADAEQVLLQTIVEATVA
                     LFEAEASSIALFERDPDRLEFRVAAGAQGAGAIGLSVPPSRGIVGFVFSTGQPLALSD
                     VLSDPRFDRATAERTGYVPRSIAAVPLVDQDSTVGVLQVLDKKTSPTFSLRDMELLAV
                     FARQAGAAIRAARVQRDTEQLMRAVLTRVGGEGAGDEDVDAVVSALSQELDTDPESPF
                     WRLVDRVAQLRDLSDREQRLVTDILEVVARHATRGRRNV"
     gene            complement(1579..1929)
                     /locus_tag="VMZ33_01050"
     CDS             complement(1579..1929)
                     /locus_tag="VMZ33_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15845.1"
                     /translation="MGNDRSEGVWKTNLGGEGATMRHGAGDDDDVEGHAKTLHASGED
                     DVEGHRQTRVSGGDDDVEGHRQTRATGGDDDDVEGHAKTLHASGDDDDVEGHAKTLHA
                     SGDDDDVEGHRQTR"
     gene            complement(2098..2817)
                     /locus_tag="VMZ33_01055"
     CDS             complement(2098..2817)
                     /locus_tag="VMZ33_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HUR15846.1"
                     /translation="MDQSVRGQIMSDPFAIQALARCPLFENATPEMLDSLANRLRRRK
                     FRRNEVIFHQGDPGDSLHVVATGAVKILLPSAEGEEAIIATLRPGDFFGELALLDGAT
                     RSATAAALEQTETLVLPRLVLMELLGTVTGLGDALLVGLARELRRLTSQVEELHFLDL
                     AGRLAMRLTRLAEESHPDNRGEIRLNWPYTQSDLAAMIGGTRQSVNKLLSQLVDEKLI
                     EIGSDELVITNLDDLARRAER"
     gene            complement(2827..4467)
                     /locus_tag="VMZ33_01060"
     CDS             complement(2827..4467)
                     /locus_tag="VMZ33_01060"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR15847.1"
                     /translation="MGIIAAYRRVLRNGPLARLLFGEFVSSIGDWLYLVALLVLIWNE
                     TQDALLLGVVGAARIVPYILLSVPAGIVADRYDRRLVLLFTDIARGLLMVLIAGAVFL
                     NMSIWVVVGLSVIATCFAAFFSPTIGAYLPSLTKDESELGPANSAWSSLDNLAFFIGP
                     AFAAALLALDNLVLAFLLNALTFAVVAVVLWRLPSARAKADETDAKAADKTVAMGLRA
                     TLAPVARPLVGIGVLNVADSFVFGGLGVITVILAVDVFAVGEEGTGLLNSAVGVGGVV
                     GAMVAGALVLRRRLAPPLLIGAFGLAIGVALLGQVGDFAIALVLMAVAAAAALLLEIV
                     ATTLQQRIVPDAVRGRTLGIIETVAVTAYAAGSFVLPVLFAAQPQAVLLACGLIMATA
                     GVLAVLLLGRYAINEPEIAPAVRGLADVALFAGLAPARLESAMRASSVREAAAGEMVV
                     RQGEAPDNFYVINSGTVEVTQTDESGAARVLRQMGSGEFFGEIGLLSKIPRTATVTAL
                     EPLTLISLPGPAFLELVSTEGSGLTYRLLDLHRGATAD"
BASE COUNT          875 a         1590 c         1549 g          704 t
ORIGIN      
        1 ccgggatgct tcgacaggat ccgcgccaca aggccacaca tgtgcggagc ggcaaacgaa
       61 ttccccgtcg caaccgtcgt cccgccgtcc ttccagccga tgggcacgtc gacaccccac
      121 gcgccccact ccaccggagg cgtcgggttg tagaaaaacg cgtagggatc cggaatcgca
      181 tgagccgcta ccgagaagac cgacgagaag gtcgacggaa agctcaaccc gggtacgttg
      241 ttgacagcgc agacgagcga cacctgcttg aagtaggcgt cgtcgacgat ctcgtggaag
      301 aacgggaaga gccgctcgct tttcgacgac aggctgaggt tcatggtgtg gacgcccttt
      361 tggacggccc actccaggcc tgcgagaaag gccgcgccct tgccgcgcag gtcggcgcca
      421 agaactcgga cggagtagat ctctacctct ggggccagtc ccacgacgat gcctgcacac
      481 gcggtcgcgt gcccgaacat gtcaaccggc tcatcgggca cgacctgtgc ctcgtcatcg
      541 atgatcacca cggccacgct ctccacgagc cggccctgga cgacggggtg cgtgccctcg
      601 acgccgctgt cgatgatcgc gaccttgacg ccggcaccgg tcgcgccgcc ccacgcccac
      661 tcgcggtcca tgaggccgtg ctgagcggcg cggatcagcc tttctcgcgc ctcgggcacg
      721 aacggctcgg accaggcggg aaggtcgtca gtcatctatg cggcgctcgg caaccgacgc
      781 tcagacgttg cggcggccac gcgtcgcgtg ccgggccacg acctcgagga tgtccgtcac
      841 aagccgttgc tcacgatccg acaggtcgcg cagctgcgcc acgcgatcca cgagacgcca
      901 gaacggcgat tcggggtccg tgtcgagttc ctggctgagc gccgacacca ccgcgtcaac
      961 gtcctcatcg cccgcgccct cgcccccgac tcgcgtcagg acggcgcgca tgagctgctc
     1021 cgtgtcgcgc tgcacgcgcg ccgctcggat ggccgcgccc gcctgacgcg cgaagaccgc
     1081 gagcagctcc atgtcgcgca gcgagaacgt gggcgaggtt ttcttgtcga gcacctggag
     1141 cacgcccacc gtcgagtcct ggtcaacgag cgggaccgcg gcgatcgagc gcggcacata
     1201 gcccgtgcgc tcggccgtgg cgcggtcaaa gcgtggatcg gagaggacgt cggacagcgc
     1261 caggggctgg ccggtcgaaa agacgaaacc cacgatgccg cggctgggtg gcaccgacag
     1321 tccgatggcg ccggcaccct gggccccggc agcgacacgg aactcgagcc ggtcggggtc
     1381 acgctcgaag agcgcgatgg acgatgcctc ggcctcgaag agcgcgaccg tcgcctccac
     1441 gatcgtctgg agcagcacct gctcggcgtc ggcgtccaga cggcgggcaa cagaagaccg
     1501 ccgcgctgcg aggcggagtg cctccagcgc ggcggggaga ccgggatcgc tcatcccggc
     1561 agttacgtca gaatgaaatc agcgggtctg tcggtgaccc tcgacatcgt cgtcgtcgcc
     1621 cgacgcgtgg agcgtcttcg cgtgaccttc gacatcgtcg tcgtcgcccg acgcgtggag
     1681 cgtcttcgcg tgaccttcga catcgtcgtc gtcgccaccg gtcgcccggg tctgccgatg
     1741 accttcgacg tcgtcgtcgc caccgctcac gcgcgtctgg cggtggcctt ccacatcgtc
     1801 ctcaccggac gcgtggagcg tcttcgcgtg gccctcgacg tcgtcgtcgt cgccggcgcc
     1861 gtgacgcatc gtggcacctt cgccaccgag gttcgtcttc cagacgcctt cgcttcgatc
     1921 gttgcccatt tctgggacct cccttgctct cgttgctttc tatctgctgg ccgaagcctt
     1981 gagcgacatt acccacgctc cggacagacc ggaagcgagg aaccgacagc acgactgtcg
     2041 gcaatccgac atcacggatg tcattgtcag cgcgtcagcg tgtttttggg aaggggttca
     2101 gcgctcagct cgacgcgcga ggtcgtccag gttcgtgatt acgagctcat cggagccaat
     2161 ctcgatcagc ttttcgtcga cgagctgcga cagcagcttg ttcacgctct gacgcgtgcc
     2221 gccgatcatc gccgcgagat cggactgggt gtacggccag ttgaggcgga tctcaccgcg
     2281 gttgtcggga tgcgactcct cggcgagccg ggtcagtcgc atcgccagtc ggccggccag
     2341 atccaggaag tgcagctcct ctacctggct tgtcagccgc cgcagctcac gggcaaggcc
     2401 gaccaacagc gcgtcgccga ggccggtcac agtgccgagc agctccatca gaaccaggcg
     2461 tggaagcacc agcgtttcgg tctgctcgag tgcggcggcc gtagccgacc gcgtcgcacc
     2521 gtcgagcagt gccagctcgc cgaagaagtc gccgggtcgc agcgtggcaa tgatcgcctc
     2581 ttcaccctca gcggacggaa gcaggatctt gaccgcgccc gttgcaacga cgtgcagcga
     2641 gtcgccgggg tcgccctgat ggaagatcac ctcattccgc cgaaacttgc ggcgccgcag
     2701 tcgattcgcc agcgaatcga gcatctcagg ggtagcgttc tcgaacaggg ggcagcgcgc
     2761 aagcgcctgg atcgcgaacg ggtcgctcat aatctggccg cggacggact ggtccattac
     2821 ctcaccctag tcggctgtag cgccacgatg cagatcaagc agccgataag tcaagcccga
     2881 gccctccgtc gagacgagct cgaggaacgc cgggccgggc aggctgatca gcgtgagcgg
     2941 ctccagcgcc gtgacggtag cggttcgagg aatcttcgaa agcaggccga tctctccgaa
     3001 gaactctcca gaacccatct gtcgcagcac cctggccgcg ccggactcgt cggtttgggt
     3061 cacttcgact gtccctgagt tgatcacgta gaaattgtcc ggcgcctcac cctgtcggac
     3121 aaccatctcg ccagcggccg cctcgcgcac ggacgaggcg cgcatcgccg actcgagacg
     3181 cgccggcgca aggccggcaa aaagcgccac gtccgcgagt ccacggacgg caggcgcgat
     3241 ctcgggctcg ttgatcgcgt aacgaccgag gagcagcacc gcaaggacgc cggctgtggc
     3301 catgatcaga ccgcaggcaa gcagcacggc ctgcggttgc gctgcgaaga gaaccggtag
     3361 cacgaaggag ccggcggcat aggcggtgac cgcgacggtc tcgatgatcc ccagggtgcg
     3421 gccgcgtacc gcgtcaggca cgatccgttg ctggagggtc gtggccacga tctcgaggag
     3481 cagcgcggcg gcagcagcga ccgccatgag cacgagggca atcgcgaaat cgccgacctg
     3541 accgagcagc gccacgccaa tggccaatcc gaaggctccg atcagcagcg gtggcgccag
     3601 ccgccggcgg aggacgagcg ctcccgctac catggcaccg accactcccc cgacgccgac
     3661 ggccgagttc agcaggccgg tcccctcctc accgacggca aagacgtcca cggcgaggat
     3721 gacggttatc acgcccagac cgccgaacac gaagctgtcc gcaacgttga gcacgccgat
     3781 tcccaccaag ggccgcgcga ccggggcgag cgtagcccgg agacccatgg ccaccgtctt
     3841 gtcggcggct ttggcgtcgg tctcgtcagc cttggcccgc gccgacggca gtcgccacag
     3901 gactacggca acgactgcaa acgtcagcgc gttcaacagg aacgccagta ccaggttgtc
     3961 cagcgcgagg agagcggccg caaatgcagg tccgatgaag aacgcaaggt tgtcgaggct
     4021 cgaccatgcg ctgttcgccg gtcccagctc tgactcgtct tttgtgaggc tcgggaggta
     4081 ggcgccaatc gtgggcgaga agaatgccgc gaagcaggtg gcgatcaccg acaggccgac
     4141 gacgacccag atcgacatgt tcaggaacac ggcgccagcg atgaggacca tgagcagtcc
     4201 acgggcaatg tccgtgaaca gtaggacgag ccggcggtcg tagcggtcgg cgacgatgcc
     4261 ggccggcact gacaacagga tgtagggcac gatccgcgcc gcgccgacca cgccgagtag
     4321 aagcgcgtcc tgcgtctcgt tccatatgag caccaggagc gcgacgaggt acagccagtc
     4381 accgatggaa gagacgaact cgccgaagag cagccgcgcc agcgggccgt tacggagaac
     4441 gcgtcggtac gcggcaatga ttcccacggc ccctcaatcg cgctttacag gtgtcggcca
     4501 ctttacatct gcgttgtcgg cacgatcatt gacctcgcgc acgcggaacg gcgatgctgg
     4561 gccgagaccg cgatcagcgg ccgctaatca gtgcaggaga tcgcgatgaa cgacgaagag
     4621 cgcaacgaaa tggacgccga gggcgccaaa acccgcggtg gcgggcagtg gtcaggtgcg
     4681 gccgaaggcg acggagacgt cgagggccac gccaagac
//
LOCUS       DATLCO010000037         4931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3768990, whole genome shotgun sequence.
ACCESSION   DATLCO010000037 DATLCO010000000
VERSION     DATLCO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3768990"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VMZ33_01065"
     CDS             <1..355
                     /locus_tag="VMZ33_01065"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HUR15848.1"
                     /translation="GMESRGFIFSAPMAMELGAGFVPVRKLGKLPAETASVEYALEYG
                     TNTLEIHKDALQPGQKVLIVDDLLATGGTVLGTIELVKTLKADVVGLAFLVELLFLKG
                     RERLSEHTIHSVVQY"
     gene            374..1678
                     /locus_tag="VMZ33_01070"
     CDS             374..1678
                     /locus_tag="VMZ33_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15849.1"
                     /translation="MGRRDEDAEGYNAERRAFFRTFGRQSVRSAGAVVGAAAELRRAG
                     GDAARELFALGQDAPNVSTTAAGPTADGDAPPDGSAAQFRSPYLVGHSGLTLLDQRVL
                     PGRLETIAVRRASEIASAIRASVVNSGPVLADIAAFGLAEIARSESPGRAAQSFDQVF
                     RAAIDTLRTARSDVHALTATLDRMQSRYEAIAVGEGDVAQELRAEAEAIVGEATTAHA
                     TLARTGAEVLAAAAAGRDDGTPGTPLDLLMHGDSGPLSCGVVGTGTALIQALIATGRS
                     VHVWVTRAEPSAEGARITSLQLAQVDVPHTVIADAAVGWLMAARSIDGVLLRGDTVVA
                     NGDVAALIGSRGVAHLAATADVAVYALAPRTAWDANAADAGRLSDPIRSTAETLAAMV
                     GNAAYGRPQSFAASLSPRVDVLPRDLISAYLTEDGPQPGGRV"
     gene            1679..2557
                     /locus_tag="VMZ33_01075"
     CDS             1679..2557
                     /locus_tag="VMZ33_01075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR15850.1"
                     /translation="MATTARPGAVDVSGRILEIATTRDRELLAEFLGRDRIRSAYALC
                     DLEDREFAKSKWGVATRSGETVAVVLEFGGLTPQPLFVSGHPEGIVSILRELIKPRIV
                     YLAADESLLDPIEHVYRIDPGPQMVRMWVDRQMFRPVHGSVMRLVPTDIVDLNRLYGL
                     GFSGWLPAESIASGVYYGVRIGGRLVAAAGTHVISPNARLAAVGNVMTQTDFRGRGFA
                     KATTSAVTQELLRTCEEVVLNVRSDNPPAIAAYRALGYREHNRFEERLAHRRGAPWDS
                     ILAHLGRLLVRNKEPA"
     gene            2554..3858
                     /gene="ahcY"
                     /locus_tag="VMZ33_01080"
     CDS             2554..3858
                     /gene="ahcY"
                     /locus_tag="VMZ33_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HUR15851.1"
                     /translation="MTTTELTRPTLQAAQLPPHDVTDIGLAADGKTRIEWAEREMPVL
                     RLIRERFEREKPLDGMRIGACLHVTTETANLMRTLKAGGAEIALCASNLLSTQDDVAA
                     ALVAEYGISVFARRGVDRDGFYSHLNAVADTHPHLTQDDGCDLVSLLHRERTDALDGV
                     LAGTEETTTGIIRLRAMAADGALKYPVVGVNEAKTKHMFDNRFGTGQSTVDGILRATN
                     ILLAGRHVVVAGYGWVGKGIASRMRGMGAQVAVVEVDPIAAIEAFMEGYQVMAGLEAA
                     AWGEVFITATGNLHVFRREHFERMTNGAIMANSGHFDAELDLVALSEMAGGRVRQLRR
                     DVKEYDMGDRRLYVIADGRLVNLAAAEGHPAAVMDMSFANQALAAEYGAKNHASLGPT
                     VHVMPVEIDREVARLKLEALNVQIDEMTPEQKRYVASWQEGT"
     gene            3866..>4931
                     /gene="metK"
                     /locus_tag="VMZ33_01085"
     CDS             3866..>4931
                     /gene="metK"
                     /locus_tag="VMZ33_01085"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HUR15852.1"
                     /translation="MTSIVDDESYLFTSESVTEGHPDKMCDQISDAILDAILKDDPSA
                     RVACETATTTGTVMVLGEITTNTYVEFQAVIRDTVRSIGYTDATYGFDYRTCGTLVSV
                     HEQSADIKRGVDEAIETRGKADQHDQGAGDQGMMFGFACRETPELMPLPITLAHRIAR
                     RLSEVRKQGLVDYLRPDGKSQVTVEYSRGVPVAVRTVVVAAQHDDDVANDRLRDDMIE
                     MVVKEVIPAELRETAPKIHVNPTGRFVIGGPMGDAGLTGRKILVDSYGGMARHGGGAF
                     SGKDPSKVDRSGAYAARWVAKNVVAAGLADRFEVEIAYGIGIARPISFSVESFGTGRI
                     PDEELQALIERTFDLRPASII"
BASE COUNT          829 a         1668 c         1640 g          794 t
ORIGIN      
        1 cggcatggag tcgcgcggct tcatcttcag cgccccgatg gcgatggagc tcggcgccgg
       61 cttcgtcccg gtgcgcaagc tgggcaagct gccggcggaa acggccagcg tcgaatatgc
      121 cctcgagtac ggcaccaaca ccctcgagat tcacaaggat gcgcttcagc ccggccagaa
      181 ggtgctcatc gtcgatgacc tcctcgcgac cggcggcacc gtcctcggaa cgatcgagct
      241 cgtcaagacg ttgaaggcag atgtggtcgg cctggcgttc ctcgtcgagc ttctcttcct
      301 caaggggcgc gagcggctga gtgagcacac gatccacagc gtcgtccagt actgacgcgc
      361 tgacggcccg ctgatggggc ggcgcgacga ggacgctgag ggctacaacg ctgagcggcg
      421 cgcgtttttc cgcaccttcg ggcgtcaatc agttcgtagc gccggcgccg tcgttggcgc
      481 cgcggcggag cttcggagag ccggtggcga cgccgcgcgc gagctatttg cactggggca
      541 ggacgctccg aacgtttcca cgaccgcagc tggaccaact gccgatggtg acgcgccacc
      601 cgatgggtcg gccgctcagt tccgcagccc ctacctcgtc ggtcattccg gtctgacgct
      661 gctcgaccag cgcgtcctgc caggtcgcct tgagaccatc gccgtccgcc gggccagcga
      721 gatcgcttcg gctatacgcg caagcgtcgt caactcaggc ccggtgctcg ccgacatcgc
      781 ggcctttggg ctggctgaaa ttgcgcggag tgagtcgcca ggccgagcgg cccagagctt
      841 cgaccaggtc ttccgcgccg cgatcgacac cctgcggacc gcccggtcgg acgtccacgc
      901 gttgaccgca accctcgatc ggatgcagtc gcgttacgag gccattgccg tcggggaggg
      961 cgacgtcgcg caggagctcc gcgcggaggc agaggccatc gttggcgagg caaccaccgc
     1021 gcacgccacg ttggcacgga ccggcgctga ggttctggcc gctgctgcgg ccgggcggga
     1081 cgatggcacg cccggcacgc cgctggacct cctgatgcac ggcgacagcg ggccactgtc
     1141 gtgcggtgtc gtgggcacgg gcaccgcgct gatccaggcg ctgatcgcaa cggggcgcag
     1201 cgtgcacgtg tgggtaacgc gcgccgagcc gtcggccgag ggtgcccgca tcacgtcgct
     1261 ccagctcgcg caggtggacg tgccgcacac agtcatcgca gatgcagcgg ttggttggct
     1321 gatggccgct cgttccatcg acggtgtgct gctgcgcggc gacaccgtcg tggccaacgg
     1381 agacgtggcc gcgctgatcg gcagccgcgg agtggcgcac ctcgccgcca ccgccgatgt
     1441 ggccgtctac gccctcgcgc cgcgcacagc atgggacgcg aacgccgccg acgccggccg
     1501 gctgagcgat ccgattcgct ctaccgccga gacgctcgcc gcaatggtgg gaaatgcggc
     1561 ttacggccgt ccccagtcct tcgccgcctc tctcagcccg cgcgtcgatg tgctgccgcg
     1621 tgacttgatc agcgcctatc tcaccgagga cgggccccag cctggcggac gcgtctagat
     1681 ggccaccacc gcgcggccag gcgccgtcga cgtcagcggc cgcatcctcg agatagccac
     1741 aacgcgcgac cgagagctgc tcgccgagtt cctggggcgt gaccggatcc gctcggccta
     1801 tgcgctgtgc gatctcgaag accgcgagtt cgccaagagc aaatggggag tggccacccg
     1861 ttctggtgaa accgtcgcgg ttgttctcga gttcggcggt ttgacgccgc agccgctctt
     1921 cgtttccggc catccagagg ggatcgtttc catcctgcgc gagctgataa agccgcgcat
     1981 cgtctacctg gccgccgacg agtccctgct tgacccgatt gaacacgtct accgcatcga
     2041 tcccgggccg cagatggtcc gcatgtgggt tgatcggcag atgttccggc ctgttcacgg
     2101 ctcggtcatg cgtctggtgc cgacggatat cgtcgacctc aaccgcctct acggtctcgg
     2161 cttctcgggc tggctgcccg ctgaatcgat cgcctcaggc gtttactacg gcgtccgcat
     2221 cgggggccgc ctggtggccg ccgccgggac ccacgtgatc agtccaaatg cacggctggc
     2281 tgccgtgggc aacgtaatga cgcagaccga cttccgcggc cggggtttcg cgaaagcgac
     2341 gaccagcgcc gtcacgcagg agcttctccg cacctgcgag gaagtggttc tcaacgtacg
     2401 atcagacaac ccaccggcca tcgccgccta tcgagcgctt gggtaccgtg agcacaatcg
     2461 cttcgaggag cggctcgcgc accgtcgtgg agcgccctgg gatagcatcc tcgctcacct
     2521 cggacgcttg ctcgtccgca acaaggagcc cgcgtgacca ccactgaatt gacccggccg
     2581 acacttcagg ccgcccagct tccgccacac gacgtcactg acatcggcct cgccgctgac
     2641 ggcaagaccc gcatcgagtg ggccgagcgc gagatgccgg ttctgcggct catccgcgaa
     2701 cgatttgagc gcgagaagcc gctcgacggc atgcgcatcg gcgcgtgtct gcacgtgacg
     2761 actgagactg cgaacctgat gcggacgctc aaggccggtg gcgcggagat cgccctctgt
     2821 gcgtcaaacc tgctgtctac acaggatgac gtcgccgcag ctcttgtggc cgagtacgga
     2881 atcagtgtct tcgcacgtcg cggcgtcgat cgcgacggct tctacagcca cctcaacgcc
     2941 gtcgcggaca cgcatccgca cctgactcag gacgacggtt gtgacctggt ttcgctgctc
     3001 caccgcgaac gaaccgatgc tctcgacggc gtcctcgccg gcacggagga gacgaccacg
     3061 ggcatcatcc gcctgcgcgc catggccgcg gacggggcgc tcaagtatcc agtcgtgggc
     3121 gtgaacgagg ccaagaccaa gcacatgttc gacaaccggt tcggaaccgg tcaatcgact
     3181 gtcgacggca tcctgcgcgc cacgaacatc ctgcttgcag gtcgccacgt ggtcgtcgcc
     3241 ggctacggct gggtcggcaa gggaattgcc tcacgcatgc gcggcatggg cgcccaggtc
     3301 gcggtcgtgg aagtcgaccc gatcgcagcg atcgaggcct tcatggaggg ctaccaggtc
     3361 atggcgggcc tggaggcagc ggcgtggggt gaggtcttca tcacggcaac gggcaacctg
     3421 cacgtgttcc ggcgcgagca cttcgagcgc atgaccaatg gcgcgatcat ggccaacagc
     3481 ggccacttcg acgccgagct cgacctggtc gcgctgagtg agatggctgg cgggcgagtg
     3541 cggcagctgc gccgtgacgt caaggaatac gacatgggcg accggcgcct gtacgtcatc
     3601 gcggacgggc ggctcgtcaa cctcgccgcc gctgagggac acccggccgc ggtcatggac
     3661 atgagcttcg ccaaccaggc tctcgcggcc gagtacggcg caaagaatca cgcgagcctt
     3721 ggcccaacgg tccacgtgat gccggtggag atcgatcgcg aggtcgcgcg gctcaagctc
     3781 gaggctctca acgtccagat cgacgagatg acccccgagc agaagcgcta cgtcgcttca
     3841 tggcaagaag ggacctgatc caaccatgac cagcatcgtc gacgacgagt cctacctctt
     3901 cacatctgaa tcggtgaccg agggtcaccc cgacaagatg tgcgaccaga tcagcgacgc
     3961 gatcctggat gcgatcctga aagacgatcc ttccgcgcgc gtcgcgtgcg agacggccac
     4021 gacgaccggc acggtcatgg ttctcggtga gatcacgacg aacacgtacg tcgagttcca
     4081 ggctgtcatt cgcgacacgg tccgctccat cggctacacg gatgccacgt acggcttcga
     4141 ctaccgaacg tgcggaacgc tggtctcggt ccacgagcag tcggcggaca tcaagcgcgg
     4201 cgtcgacgag gcgatcgaga cacgcggcaa ggccgaccag cacgaccagg gcgcgggcga
     4261 ccaggggatg atgttcggct tcgcgtgccg cgagactccg gagctgatgc cgctgccgat
     4321 cacgctcgcg catcgcatcg cgcgacggct ttccgaggtc cggaagcagg gcctcgtcga
     4381 ctatctccgc cccgacggca agtcgcaggt caccgtcgag tacagccgcg gcgtgcccgt
     4441 cgccgtgcgg acggtcgtgg ttgcggcgca acacgacgac gacgtcgcga atgaccggct
     4501 gcgcgacgac atgatcgaga tggtcgtgaa ggaggtcatc ccggccgagc tccgcgagac
     4561 ggcgccaaag atccacgtca acccgaccgg ccgcttcgtc atcggcgggc cgatgggtga
     4621 tgcaggtctc acagggcgga agatcctcgt ggactcgtac ggcggcatgg ctcggcacgg
     4681 cggcggcgcc ttctcgggca aggatccgtc caaggtggac cgctccggcg catacgcggc
     4741 acgctgggtc gcgaagaacg tcgtcgcggc cggtctcgcc gatcggtttg aggtcgagat
     4801 cgcgtacggc atcgggatcg cccgaccgat ctccttctcg gtcgagtcat tcggcacggg
     4861 ccggatcccg gacgaggagc tccaggcgct catcgagcgg acgttcgacc tgcgcccggc
     4921 atcgatcatc t
//
LOCUS       DATLCO010000038         3661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3771582, whole genome shotgun sequence.
ACCESSION   DATLCO010000038 DATLCO010000000
VERSION     DATLCO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771582"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VMZ33_01090"
     CDS             <1..198
                     /locus_tag="VMZ33_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15853.1"
                     /translation="QSRKALTGIGATEEGTLRNHMLYESGRRRHSVYFSVIEEEWPRV
                     RVHLEQRLARLKTPKEPPPVP"
     gene            complement(164..1963)
                     /locus_tag="VMZ33_01095"
     CDS             complement(164..1963)
                     /locus_tag="VMZ33_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HUR15854.1"
                     /translation="MALRIEDYALIGDTHAAALVGYDGSIDWLCLPRFDASACFAALL
                     GNTDNGRWLLAPVGVERATSRRYHDESLVLETTWETESGTVRVTDFMPPRTVEPDVYR
                     IVEGVSGEVDMRTELVLRFDYGRLVPWVRRVGADTLAVAGPDAVAIRADVELRGEDLK
                     TVAEFTVHPGETVTFVMRWHPSHLPATEPEDAMSMRFRTVEWWREWSSRMRYEGPWRE
                     EIGQSLRVLKALTYGPTGGIVAAATTSLPEQIGSIRNWDYRYCWLRDAAFSLWALHIG
                     GYTEEAQSYVDWLLRAAGGDPDALQTVYGPAGEGRLPELELDWLAGYEGSRPVRIGNA
                     ASMQFQLDVYGEVLDTLHLARQMGMSWDPETWALARRLVEYVVQHWQEPDEGIWEVRG
                     PRQHFTHSKVMAWVALDRAVRAIEKFGLDGPLEEWQRVRQQIHDDVCSKGFDAERNTF
                     TQHYGSKELDASLLMIPLVHFLPATDPRMLGTIAAIQRDLMVDGFVLRYPTDLTNDGL
                     PAGEGAFLACTFWLVDNLALIGQIDEATRLFERLLELRNDVGLLSEEYDPTDKRLLGN
                     FPQAFSHVGLVNSAFNLDRAMRARARAEAPSAS"
     gene            1988..2155
                     /locus_tag="VMZ33_01100"
     CDS             1988..2155
                     /locus_tag="VMZ33_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15855.1"
                     /translation="MATTDTTADQKIADADLIRAGRRRLMIDTLVIAVSAGYGAAMQR
                     AIGVPRRNLQA"
     gene            2152..2913
                     /locus_tag="VMZ33_01105"
     CDS             2152..2913
                     /locus_tag="VMZ33_01105"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HUR15856.1"
                     /translation="MSAPSRRHHQDHPTWSRASGVVFGILLIAIGAVLLAVNTFGMVL
                     PFELGRVGWPVFVIAPGVVLLVAGLLTDNDAGIGLSVAGGMVTMVGAILAYQSATDHW
                     SSWAYAWALIAPTAVGAAMLLWGIFHGRMGVVRQGVSGLGLGLVLFLVGFGFFEGVLN
                     IGGERGLAGLGRQALPVALILVGVLLVISRLMPSREPRRVASDWAPAGGPNAYGIQPP
                     AATPTPPGPQGHGPLPPTPAPWDRPAPPPAPAQHE"
     gene            complement(2945..>3661)
                     /locus_tag="VMZ33_01110"
     CDS             complement(2945..>3661)
                     /locus_tag="VMZ33_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HUR15857.1"
                     /translation="HDFLRARGFKTAEKTLGQRSSFRVDLTRPEEEIFASFSKGHRAD
                     TKRAMREGVVVREATEESEVDWLHRMLGATTERKAFEFHAASYYRALWRHFGDAASLF
                     LAEWKGEVVAATLNIAWGDTGLYLFAGSTRSGLDSRAGHILQWESIRWAKARGATTWD
                     MWGIADARGRMELLRAAGVADDAPEMTALDAEARRDPKDGLDRFKKGWGGYAVRVVPA
                     YDKVFIPPAYWYWTRRRGEA"
BASE COUNT          587 a         1258 c         1199 g          617 t
ORIGIN      
        1 cagtcacgga aggcgctgac cgggatcggc gcgacggagg aggggacgct ccgcaaccac
       61 atgctgtacg agagcggccg gcgccggcac tcggtgtact tcagcgtgat cgaggaggag
      121 tggccacgcg tccgggtgca cctcgagcag cggctggcgc gcctcaagac gccgaaggag
      181 cctccgcccg tgccctagcg cgcatcgcgc ggtcgaggtt gaatgccgag ttcacgagac
      241 caacgtggct gaacgcctgc gggaagttgc cgaggagccg cttgtcggtc gggtcgtatt
      301 cctcagacag caacccgacg tcgttgcgca gttcgagcag tcgctcgaaa agtcttgtcg
      361 cctcatcgat ctggccgatg agcgccaggt tgtcgacaag ccagaaggtg catgccagga
      421 acgccccttc gccggcaggc aggccatcgt tggtcaagtc ggtcgggtag cgcaggacaa
      481 agccgtcaac catgaggtcg cgttggatgg ccgcgatcgt ccccagcatc cgggggtccg
      541 tggccggcag gaaatggacc agcgggatca tcaacaggct cgcgtccaat tctttggagc
      601 catagtgctg cgtgaacgtg ttgcgctcgg cgtcgaagcc ctttgagcag acgtcgtcgt
      661 ggatctgctg ccgaacccgt tgccattcct ccagcgggcc gtcgagcccg aacttctcga
      721 tcgcccgcac tgcccggtcg agggcgaccc atgccatcac cttcgaatgc gtgaagtgct
      781 ggcgtggccc gcgtacttcc cagatgcctt catcgggctc ctgccagtgc tggaccacgt
      841 actcgacgag gcggcgcgcc agcgcccacg tctctggatc ccagctcatg cccatctggc
      901 gcgccaggtg gagcgtgtcc agcacttcgc cgtagacgtc aagctggaac tgcatcgagg
      961 ccgcgttccc gatgcggacc ggtcgcgagc cctcgtaacc tgcgagccag tcgagctcga
     1021 gctctggtag ccgcccttcg ccggcgggtc catagaccgt ctgcagggca tcgggatcgc
     1081 cacccgccgc tcgcagcagc cagtcgacgt agctctgtgc ctcctcggtg tagccgccga
     1141 tgtgcagcgc ccacagcgag aacgccgcgt cacgcagcca gcagtagcgg taatcccagt
     1201 tgcggatgct gccgatctgc tcggggagtg acgttgtcgc ggcggcgacg atgccgcccg
     1261 tcggcccata ggtcagtgcc ttgagcacgc gcagggactg gccgatctcc tcgcgccacg
     1321 gaccctcata gcgcatccgc gacgaccact cgcgccacca ttcgacggtg cggaagcgca
     1381 tcgacattgc gtcctccggc tccgtggccg gcaggtgcga cggatgccag cgcatcacga
     1441 acgtaacggt ctcgcccggg tggaccgtga actcggcgac tgtcttcaga tcctcgccgc
     1501 gcaactcaac gtctgcgcgg atcgccacgg cgtctggccc ggccacggcc aacgtatccg
     1561 cgccgacgcg ccgcacccac ggcacgagac ggccgtagtc gaaacgcaga acgagctcgg
     1621 tgcgcatgtc gacttcgccc gacacccctt cgacgatgcg atacacgtcg ggctcgacgg
     1681 ttcgaggcgg catgaagtcg gttacgcgga ctgtgccgga ctcggtctcc cacgtcgtct
     1741 cgagcacgag cgactcatcg tgatagcgac ggctcgtcgc gcgttcgaca ccgaccggcg
     1801 caagcagcca gcgcccgttg tcggtgtttc cgagcagcgc cgcgaagcac gcggacgcgt
     1861 cgaatcgcgg cagacacagc cagtcgatgg acccgtcgta gccaacgagc gccgccgcgt
     1921 gcgtgtcgcc gatgagggcg taatcctcga tccgcagcgc cactgtccag aatctagccc
     1981 agagagaatg gccacgaccg acacgacagc cgaccagaag attgcagacg cagatctgat
     2041 ccgcgccggc cgccgacgcc tgatgatcga cacgctggtg atcgcggtgt cggcgggcta
     2101 cggtgccgcc atgcagcgcg cgatcggtgt accgcggcgt aatctgcaag cgtgagcgct
     2161 ccgtcacgcc gccaccacca agatcacccc acgtggtcgc gcgcctccgg agtcgtgttc
     2221 ggcatcctcc tgattgcgat cggcgctgtt ttgctggccg tcaacacctt cggcatggtg
     2281 cttcccttcg agctcggccg cgtgggctgg ccggtcttcg tcatcgcgcc gggcgtggtg
     2341 ctgctcgtcg ccgggctcct gacggacaac gacgcgggca tcggcctgtc agtggctggt
     2401 ggcatggtca cgatggtcgg cgccatcctc gcgtatcaat cggccacgga ccactggtcc
     2461 agctgggcct atgcctgggc gctcatcgcc ccaactgcgg tcggcgccgc gatgctgctg
     2521 tggggcatct ttcacggccg tatgggcgtc gtcaggcagg gtgtcagtgg cctcggtctg
     2581 gggctcgtgc tgttcctcgt cggctttggc ttcttcgagg gcgtgctcaa catcgggggc
     2641 gagcgtgggc tagccggcct tggccggcag gcactccccg tggccttgat cctggtcggg
     2701 gtgttgctcg tgatctcacg gctcatgcca agtcgtgagc cgcgccgcgt tgcctccgat
     2761 tgggcaccgg ccggcgggcc gaacgcatat gggattcagc cgccggctgc cacgccgacc
     2821 ccgccggggc cgcaagggca tggacctcta cccccaacgc ctgcgccgtg ggacaggccc
     2881 gcgccgccgc ccgcgccagc gcaacacgag tgaacgatcc cttcgccgac cgcgagggac
     2941 ggtcctaggc ttctccccgt cggcgggtcc aataccaata ggccggtggg atgaacacct
     3001 tgtcgtacgc cgggacgacg cgaactgcgt aaccgcccca gcccttcttg aaccgatcca
     3061 agccgtcctt ggggtcgcgc cgcgcctcgg catcgagcgc ggtcatctct ggagcgtcgt
     3121 ctgcgacacc tgcggcccgc agcagctcca ttcgtccgcg cgcgtcggcg atgccccaca
     3181 tatcccacgt ggttgcaccg cgcgctttgg cccagcgaat cgattcccac tgcaggatgt
     3241 gcccggctcg gctgtcgagg ccggagcgcg tcgaaccggc gaacaggtag aggccagtgt
     3301 cgccccatgc gatgttgagc gtcgcggcca cgacctcgcc cttccactcg gcaaggaaga
     3361 gcgacgcggc atcgccgaaa tgacgccaaa gcgcgcggta gtaagaggcc gcgtggaact
     3421 cgaacgcctt gcgctcggtc gtcgcgccca gcattcggtg aagccaatcg acctctgatt
     3481 cttcggtcgc ctccctgacg accacgcctt cgcgcatggc gcgcttcgtg tcggcgcgat
     3541 ggcccttcga gaagctggcg aagatctctt cctcgggacg tgtgaggtcg acccgaaacg
     3601 acgaccgctg gccgagcgtc ttctcggcgg tcttgaagcc acgcgcccgg aggaagtcgt
     3661 g
//
LOCUS       DATLCO010000039         4659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_790564, whole genome shotgun sequence.
ACCESSION   DATLCO010000039 DATLCO010000000
VERSION     DATLCO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790564"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VMZ33_01115"
     CDS             complement(<1..351)
                     /locus_tag="VMZ33_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HUR15858.1"
                     /translation="MTVTALATETTAAPDFAPLPVPERLPTEPEVIFVVGVARSGTTM
                     MRNILATSPRVAVARETHFMGHIFNRPGARHFFRKAGDLTSDDSVRKVADLIYSGEYL
                     RQSRFGNISPYWRWL"
     gene            complement(760..1323)
                     /gene="cysC"
                     /locus_tag="VMZ33_01120"
     CDS             complement(760..1323)
                     /gene="cysC"
                     /locus_tag="VMZ33_01120"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HUR15859.1"
                     /translation="MPATSSRGACLWFTGLSGAGKTTTAERLLELLAQRRTVTFLDGD
                     VVRTHLSRGLGFSREDRDENVRRVGFVASEVVRHGGLVVCALVSPYRSTRGEVREMMP
                     EGAFIEVFVDTPLDVVAQRDVKGLYAAEKRGEVKQMTGVTDPYEPPLAPEIHLETVDR
                     TVDENAQAVIDDLERQSILQADVIAPS"
     gene            complement(1340..3004)
                     /locus_tag="VMZ33_01125"
     CDS             complement(1340..3004)
                     /locus_tag="VMZ33_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HUR15860.1"
                     /translation="MALVRRAGDPLTAKKRSAWVPPDHGSDRGGLQRKVASGLKWTLL
                     DTWGSQLLQLAVFFTLAGLLTPVDFGLVALAAVFVAFAQLLVDQGLGDALVQRRALTP
                     SQIDTAFWTAVLTGSVLCVAMIALSIPISIALDEPDLAPILQVLSFVFVIVAFTSVQS
                     SLARRQMSFRTLAIRRLIAIAGGGAVGIAAALNGLGAWSLVAQQLSAGVISVIALWAV
                     SPWRPSFTFSRADFRELSSFGANIVGGDILQFLSRNTDNFLIGVFLGAGPLGLYALGY
                     KLLETSQIVLVNSFGKVVYPTFARLAHDRERLRRAYTRLTRVVSAFTLPGYIGLALVA
                     EQATRLLLPERWADSGSIAAILLLVGPSVTVFGYGGQVLNAVGRPDITLRLRLITAIV
                     NVLGFLVAVLIFNNVYAVAIAYVLRSYLLAPLTLFWLRDYAGIPIRENLLRLRGVVPA
                     TLLMALAVLAVRTWLPVGGVAKLAIEIAVGIMVYAASLLILDRTLISELFDFARDMLP
                     GRKRPRKPDDDTTDNVRGDAELTPEQAAYLEPMRTNPTLADEVDEI"
     gene            complement(2971..3969)
                     /locus_tag="VMZ33_01130"
     CDS             complement(2971..3969)
                     /locus_tag="VMZ33_01130"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HUR15861.1"
                     /translation="MSASPTVDARPERAGRETTRRPDVFIVGAPKTGTTSLYEYLQEH
                     PDVFMSPVKEPSYFSPDVRVRPARRYQYRYPEDQAKYDELFADARDEKRLGEASTTYL
                     MSVGAPGRIHEYSPDARIIAMVRNPVDLVYSLHNERVSNGLEWMTDFEQAMAADEARR
                     RGEDLPHNLEGFGVAYRDNAYLGEQLQRWLDEFGPDRVHVILFDEFAKDTPGHYRRVL
                     EFLDVDPDFQPASFAVHNVSHRRRGGIVQGLFANPVGRFVSRKLLPKVLGQARTARNL
                     RRVRQSPLMRRKHERQPLRPELRKQLQDEFTPDVELLGRLLGRDLRSLWFGERETR"
     gene            complement(3966..>4659)
                     /locus_tag="VMZ33_01135"
     CDS             complement(3966..>4659)
                     /locus_tag="VMZ33_01135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUR15862.1"
                     /translation="VVTLPKNGGCGAARNFGVLSANTEWLAFLDADDEWEPTFLEEVL
                     DGIEKFNADFGSSGGVRKLEYKNPPTTSTRLLDGPPEAIDLTDQFWRQALKFMPIHPS
                     ALVIRRSLNNEVGGWCGEVRNGEDTPLYAEAWRKGRFVFVNKPLFTSIAPMTGVSAAF
                     IPYHDIRVSLGRIGRSAIRSLLARKRGSVWLLAWWLTAVARRHALYLVRRFKLGKLIG
                     RVRSRGAATARS"
BASE COUNT          847 a         1488 c         1579 g          745 t
ORIGIN      
        1 cagccagcgc cagtagggac tgatgttccc gaagcgcgac tggcggaggt actcgccgga
       61 gtagatcagg tcggcgacct tgcgaacgga gtcgtcgctg gtcagatcgc cggccttccg
      121 gaagaagtgg cgcgcgccgg gccggttgaa gatgtggccc atgaagtgcg tctcacgggc
      181 gacggccacg cgtggcgagg tcgcgaggat gttgcgcatc atggtcgtgc cgctacgcgc
      241 gacgccgacg acgaagatga cctcaggttc cgtgggcagc cgctcgggga ccggcagcgg
      301 cgcgaaatct ggcgccgcgg tcgtctcagt ggcaagggcg gtcacggtca ccgctggaac
      361 tcaaaggaca cggcgaaaac tccgtcagaa gttagggggt gaagggcgga agtctagcct
      421 attgagccgg acggaccaac cgcgcggccc aactctggtc cccgaccggg cggcctccgc
      481 catcgggtat cgcccacatc attagccgag cagccgcgag gtcgattagg tctgcagctc
      541 gcctcgcagc cgatcttgga cgcgtagaaa acggccaatc acgcctggct gagcacgcaa
      601 aaccagccag tcctcgtagc gaggtccccc aagtccctcg aaaacgccac taccgcgcgc
      661 cctcgtgcgc ccgtaatgat gtgagcgata tcggcgcacc caaggtgtcg gccgggccgc
      721 cggtgtcgtc cagtgcaggc gcattcggcg tccgcgtcct tagctaggcg cgatcacgtc
      781 ggcttggagg atgctctgcc gctccaggtc gtcgatgacg gcctgggcgt tctcgtcaac
      841 cgtccgatcg acggtctcca ggtggatctc gggcgctaga ggcggctcgt atgggtcggt
      901 tacaccggtc atctgcttga cctcgccacg cttctcggcg gcgtacaagc ccttcacatc
      961 gcgctgtgcg accacgtcga gtggcgtgtc cacgaacacc tcgatgaacg cgccttcagg
     1021 catcatctct cgcacctcgc cgcgggtcga gcggtacggg ctgaccagcg cacagactac
     1081 gagtcccccg tggcgcacca cctccgaagc cacgaatccg accctccgaa cgttctcgtc
     1141 ccgatcctcg cgcgagaagc cgaggcctcg tgacaggtga gttcggacga cgtcgccgtc
     1201 gaggaaggtg accgtacgtc tctgggcgag aagttccagg agccgttcgg cggtcgtggt
     1261 cttgcccgct ccggagaggc cggtgaacca gaggcacgcg ccgcggctgg aggttgctgg
     1321 catccgcttg atcatagggt cagatctcat ccacctcgtc ggcgagcgtt ggattggtcc
     1381 gcatgggttc gaggtatgcc gcttgctccg gagtgagctc ggcgtcgccg cgtacgttgt
     1441 cggtcgtgtc gtcgtccggt ttgcgcggac gcttcctgcc cgggagcatg tcgcgcgcga
     1501 agtcgaacag ttcactgatc agcgttctgt ccaggatcag caggctggcc gcatacacca
     1561 tgatgccgac tgcgatttcg atcgcaagct ttgccacgcc accgaccggc agccacgtcc
     1621 ggacggcgag cactgcgagc gccatcagga gggtcgccgg aacaacgccg cgcagcctca
     1681 gaaggttctc gcggatcggg atgccggcgt aatcgcgcag ccagaagagg gtcagggggg
     1741 cgagaaggta gctgcgcagg acatacgcga tagcgacggc gtagacgttg ttgaagatca
     1801 ggacggcaac caggaagccg agcacgttga cgatcgccgt gatcaatcgc agacgcaggg
     1861 tgatgtccgg ccggccgacc gcgttgagta cctggccgcc gtagccgaag acggtgacgc
     1921 tcggaccgac gagcaacagg atggccgcga tgctgccgct gtccgcccag cgctcgggca
     1981 ggagcagccg tgtggcctgc tcggcgacga gagccaggcc gatgtagccc ggcaacgtaa
     2041 acgcgctcac cacccgcgta aggcgtgtgt aggcgcgccg caggcgctcc cggtcgtggg
     2101 caagccgcgc gaacgtcgga tagaccacct tcccgaacga gttgacgagc acgatctggc
     2161 tggtctcgag gagcttgtat ccgagcgcgt agaggcccag cggtcccgcc ccgaggaaca
     2221 cgccgatcag gaagttgtcg gtgttgcggc tgaggaactg gaggatgtcg ccgccgacga
     2281 tgttggcccc gaacgaggac agctcacgga agtcggcgcg tgagaaggtg aagctcggcc
     2341 gccatgggct gacggcccag agggctatca ccgagatgac gccagcgctc agctgctggg
     2401 cgacgagcga ccacgcgccc aagccgttga gggctgcggc aatccctacc gcgccgccgc
     2461 cggcaattgc tatcagccgg cgaatggcca gggttcggaa tgacatctgc cgccgggcca
     2521 gggacgactg aacgctcgtg aaggcgacga tcacaaacac gaacgaaagg acctgcagga
     2581 ttggagcgag atccggctcg tcgagggcga tcgagatcgg gatcgacagc gcgatcatgg
     2641 ccacgcacag cactgagccg gttagcaccg ccgtccagaa cgccgtatcg atctgagaag
     2701 gcgtcagggc tcgacgctgc accaacgcat cgcccagacc ctggtcgacc agaagctgcg
     2761 caaaggcgac gaagaccgct gccagggcga caagtccgaa gtcgaccggg gtcaggagcc
     2821 cggcaagcgt gaagaagacc gcgagctgca ggagttggct gccccaggtg tcgagcagcg
     2881 tccacttcag cccgctcgcg accttgcgtt gcaggccacc ccggtcgctc ccatgatcgg
     2941 gcggaaccca agcggagcgc tttttggccg tcagcgggtc tcccgctcgc cgaaccagag
     3001 cgatcgcagg tcacggccga gcaggcgccc aagcagctcg acatcgggcg tgaactcatc
     3061 ctggagctgc tttcgcagct caggtcgcag cggctggcgc tcatgcttgc ggcgcatgag
     3121 cggactttgc cgaactcgcc gcagattccg tgcggtgcgt gcctgaccca gaaccttggg
     3181 caggagcttg cgcgacacga atcggccaac cgggtttgcg aacaagccct ggacgatgcc
     3241 gccgcgacgg cggtggctca cgttgtgcac cgcgaaagac gccggttgga agtcgggatc
     3301 gacgtcgagg aattccagca cgcggcggta gtgaccgggc gtgtccttgg cgaactcgtc
     3361 gaagaggatc acatggaccc ggtcggggcc aaattcgtcg agccagcgct ggagctgttc
     3421 gcccagatac gcgttatccc ggtaggccac accgaaccct tccaggttgt gtggcaggtc
     3481 ctcaccgcgc cggcgggcct cgtcggctgc catcgcctgc tcgaagtcag tcatccactc
     3541 gaggccgttc gagacccgct cgttatgcag cgaatagacc aggtcgaccg ggtttcgaac
     3601 cattgcgatg atccgcgcgt cgggcgagta ttcgtggatg cggcccggcg cgccgaccga
     3661 catgaggtag gtcgtcgacg cctctccgag tcgtttctcg tcccgggcat ccgcgaagag
     3721 ctcgtcgtac ttcgcctgat cctcggggta gcggtactgg taacgacgcg cgggtcggac
     3781 cctcacatca ggcgaaaagt agctcggctc cttgaccggc gacatgaaga cgtcgggatg
     3841 ctcctggagg tattcgtaga gcgacgtcgt gcctgtcttc ggcgcgccga cgatgaatac
     3901 gtcgggccgc cgggtcgtct cccgtccagc ccgctcgggc cgagcgtcga cggtcggaga
     3961 tgcgctcacg agcgtgctgt cgcggcaccg cgcgagcgga cgcgaccaat cagcttcccg
     4021 agcttgaacc ggcgaaccaa atacagtgcg tgccgccgcg ccacggcggt gagccaccac
     4081 gcgagcagcc agacgctgcc gcgcttgcga gccaggagcg aacggatcgc gctgcggccg
     4141 atgcgtccga gcgagacgcg gatgtcgtgg tacgggatga aggccgcgga gacaccggtc
     4201 atcggcgcga tcgaggtgaa gagcggcttg ttcacgaaca cgaagcggcc cttgcgccat
     4261 gcttcggcgt agagcggcgt gtcctcgccg ttacgcacct cgccgcacca gccacccacc
     4321 tcgttattca gcgagcgacg gatgacgagg gccgaaggat ggatgggcat gaacttcaac
     4381 gcctggcgcc aaaactgatc ggtcaggtcg atggcctccg gcgggccgtc gagtagccgc
     4441 gtcgaggtcg tgggtgggtt cttgtactcg agcttgcgga cgccgccact cgacccgaag
     4501 tcagcgttga acttctctat gccgtccaga acctcttcga ggaacgtggg ctcccattcg
     4561 tcgtcggcgt cgaggaacgc cagccactcg gtgttcgccg acaggacgcc gaagttgcgc
     4621 gccgcgccgc agccgccgtt cttgggcagc gtgacgacg
//
LOCUS       DATLCO010000040         2851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2282259, whole genome shotgun sequence.
ACCESSION   DATLCO010000040 DATLCO010000000
VERSION     DATLCO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282259"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VMZ33_01140"
     CDS             <1..566
                     /locus_tag="VMZ33_01140"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15863.1"
                     /translation="ITHALDQIQTEEVRINILHEGTGDINENDINLASASNAIVVGFN
                     AKLDPQARRTSEAEGVDVRLYDIIYRLTEEMTAALHGLLEPEEVEVVEGRVEVRQIFR
                     SGRTVIAGCYVTEGRIIKGGARVYRGGQLITTDRIESLRRFRDDVREVLAGYECGIGL
                     ADYNEAEEGDIIECFSKQMVSRVVTQA"
     gene            660..1067
                     /gene="rbfA"
                     /locus_tag="VMZ33_01145"
     CDS             660..1067
                     /gene="rbfA"
                     /locus_tag="VMZ33_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HUR15864.1"
                     /translation="MSQRTARVDELLREEISSILQREIDDPRIGFLTITDVEVSRDLS
                     HANVWVSVIGSADEKKLTLRALSHAMPFVRQRLGKLRLRRIPALHVKEDHTAERGTRL
                     LKILDDLEQGGEGAVPDQADTLPSPTGQEVSDK"
     gene            1064..2101
                     /locus_tag="VMZ33_01150"
     CDS             1064..2101
                     /locus_tag="VMZ33_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HUR15865.1"
                     /translation="MTIAPDLVPLSSLTREDVLAVPREVVDRIRGARNVLAVCHENPE
                     ADALGSALAVALAVEELGGRATPVCADVPPPMYDFIPRTDLFRQDPDPDIEYDLIVVG
                     DCGDLERVGSVLGRNAELFGRVPIIDIDHHISNPGFGVVDWIDASAAATCEMNTLLMP
                     ALGVPLTAADGAIAAALIAGVVIDTANFQHPNVTARTLRVAAELVNAGAPLAQTARLL
                     YRTKPNRQLKLFGLVLSRTQTDNDGRLVWTSLHDGDYAATGAGPEDAEGLSDLLSQSE
                     EGQLVIVFRANGQRTKISVRTRDGGLDATELTKKFGGGGHARAAGATIDLPLDEAIAA
                     VLAESRPMVSP"
     gene            2117..>2851
                     /gene="truB"
                     /locus_tag="VMZ33_01155"
     CDS             2117..>2851
                     /gene="truB"
                     /locus_tag="VMZ33_01155"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HUR15866.1"
                     /translation="MVVEAVPALDGILVVAKAPGPTSHDIVGLVRRLTGVKRVGHGGT
                     LDPFAAGVLPVFLGHATRLVEYHLSDEKEYRALVCFGARSTTDDLDGELTPTGVTTVR
                     ATVEAEMESFRGQIEQVPPDYSAVRVAGRKAYELARHGEKPTLRARTVTIHRFELVGW
                     DAADPEKPVATIEVRCSAGTYVRSLARDLGERLGTGAYLGALTRTASGPFTIDQARPT
                     DEVRAALADGRASELLLPMDTGLETFP"
BASE COUNT          502 a          974 c          936 g          439 t
ORIGIN      
        1 ccattacgca tgcgctcgat cagatccaga ccgaagaggt ccggatcaac atccttcatg
       61 agggcacagg cgacatcaac gagaacgaca tcaacctcgc ttctgcgtcc aacgcaatcg
      121 tcgtcggctt caatgcgaag ctcgatccgc aggcacgccg cacgtcagag gctgagggcg
      181 tcgacgtccg tctctacgac atcatctatc gcctcacgga ggagatgacc gcggcactcc
      241 acggcctcct cgagcccgaa gaggtggagg tggtcgaggg tcgcgtcgaa gtccgtcaga
      301 tcttccgcag tggccgcacg gtcattgccg gctgctacgt gaccgagggg cgaatcatca
      361 agggcggcgc ccgcgtctac cgtggcggac agctgatcac caccgaccgc atcgagtcgc
      421 tgcggcgctt ccgcgacgac gtgcgcgaag tccttgccgg ctatgagtgc ggcatcgggc
      481 tggccgacta caacgaggcc gaagagggcg acatcatcga gtgcttctcg aagcagatgg
      541 tgtcgcgcgt agtcacgcaa gcatagggtg cgagacgcgg cgagccgcgg atcggaccgg
      601 ggcgcgcagc agcgccaaag gcgactgcgg aggtcacata agttgactag gagccggcag
      661 tgagccagcg cacggcacgt gtcgacgagc tgctgcgcga ggagatcagc tcgatccttc
      721 agcgggagat cgacgacccg cgcatcggct tcctcaccat caccgacgtc gaagtatcac
      781 gggacctcag ccacgcaaac gtgtgggtgt cggtgatcgg cagcgccgat gagaagaagc
      841 tcacccttcg cgctctgtcg cacgccatgc cgttcgttcg gcagcggctg ggcaagctgc
      901 gcctgaggcg aattcccgcg ctgcacgtca aggaagacca cacggctgag cgcgggaccc
      961 ggctgctcaa gattctggac gacctggagc agggcggcga gggcgccgtg cccgatcaag
     1021 ccgacacgct gcccagcccg accggacagg aggtctccga caagtgacga ttgcgcccga
     1081 cctcgtgccg ctttcctcgc tcactcgaga agacgtgctg gccgtgccgc gggaggtcgt
     1141 cgaccggatc cgcggcgccc ggaacgttct cgccgtgtgc cacgaaaacc cagaggcgga
     1201 cgctcttggc tcggccctcg cggttgcgct ggccgtggag gagcttggtg gccgcgccac
     1261 acccgtctgc gccgacgtgc cgccgccgat gtacgacttc attccgcgca ccgacctctt
     1321 ccgacaggat cccgatccgg acatcgaata cgacctcatc gtcgtcggcg actgcggcga
     1381 cctcgagcgg gtcggctccg tcctcggccg caacgccgag cttttcggcc gggtgcccat
     1441 catcgatatc gaccatcaca tcagcaaccc cggcttcggc gtggtcgact ggattgatgc
     1501 ctccgccgcg gctacctgcg agatgaacac cctgctcatg cccgcgctgg gcgtcccgct
     1561 gacggccgcc gacggggcca tcgccgccgc gttgatcgcc ggcgttgtca ttgacaccgc
     1621 gaatttccag caccccaacg tgactgcgcg gaccctgcgc gtcgcggcgg agctggtcaa
     1681 cgccggtgcg ccgctggccc agacggccag gctgctctac cgaacgaaac ccaatcgcca
     1741 gctcaagctc tttggcctcg tcctgtctcg aacgcagacg gacaacgatg ggcggctcgt
     1801 gtggacctcg ctccacgacg gcgactacgc cgcgaccggt gcggggccgg aggacgccga
     1861 gggactgagc gacctcctaa gccagtcgga agagggtcag ctcgtcatcg tcttccgcgc
     1921 caacggccaa cgcaccaaga tcagcgttcg cacacgcgac ggcggcctcg acgctaccga
     1981 gctgacgaaa aagttcggcg ggggagggca cgctcgggcg gccggtgcca cgatcgatct
     2041 gccactcgac gaggccatcg ccgcggtcct cgccgagtca cggccgatgg tcagccccta
     2101 gcgaaccagt cgagaggtgg tcgtcgaagc cgtgcccgcg ctcgacggca tcctcgtggt
     2161 ggccaaggcg cccggtccca cgtcgcatga catcgttggt ctcgtccggc gcctgacagg
     2221 ggtcaagcgc gttggccacg gcggcaccct ggaccccttc gctgccggcg tgctgcccgt
     2281 cttcctggga cacgccacgc gcctcgtcga atatcacctg tccgacgaga aggaataccg
     2341 ggcattggtg tgcttcggtg cgcggtcaac gaccgacgat ctggacggcg agctgacgcc
     2401 aaccggcgtc acgaccgtgc gcgcaaccgt cgaagccgag atggagtcgt tccgcggtca
     2461 gatcgagcag gtgccgcccg actattcggc ggtgcgcgtc gccggcagga aggcctacga
     2521 gctggcgcgc cacggtgaaa agccgaccct gcgagcgcgc accgtgacaa tccatcgctt
     2581 cgagcttgtc ggatgggacg cggcagatcc cgaaaagccg gttgctacga tcgaagtgcg
     2641 ctgctcggcg ggcacatacg tgcgttcgct tgcccgtgac ctgggcgaga ggctgggcac
     2701 gggcgcctac ctcggcgcgc tgacgcgtac cgcgagcggg ccgttcacca ttgaccaggc
     2761 gcgcccaacc gacgaagttc gcgccgcact cgcggatggt cgcgccagcg agctgctgct
     2821 gccgatggat accggcctgg agaccttccc g
//
LOCUS       DATLCO010000041         3915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1538397, whole genome shotgun sequence.
ACCESSION   DATLCO010000041 DATLCO010000000
VERSION     DATLCO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538397"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1557
                     /gene="ffh"
                     /locus_tag="VMZ33_01160"
     CDS             223..1557
                     /gene="ffh"
                     /locus_tag="VMZ33_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HUR15867.1"
                     /translation="MFDDLSDRLRGVLNKLSGHGRVTEVDVDAAMREVRLALLEADVN
                     YKVVKDFVARVRERAVGTEVLDSLNAGQQVIKIVHEELTAILGAGDRTFRLAGSPAVV
                     MLVGLQGSGKTTTAAKLTRVVTKQGRRPLLVAADPYRPAAADQLQALGRQLDVPVHRG
                     PPNEKVLDTVRNGLAVARQTGRDVVIVDTAGRTSVDEALLEEIRALAAATNPSETLLV
                     VDAMVGQESVSVAQAFVGVAPVTGLVLTKIDGDARGGAALSISAVTGLPVKFMGTGEK
                     TDALEPFHADRLAGRILGMGDVLTLVERAQETFDEKQAEEMARKLQRNQMTLEDFLSQ
                     MQQLKRMGSIGGLLEMIPGMGGAAKEAQQAVDRGDLKRVEAIIQSMTPRERREPDILS
                     SSRRKRVARGSGTTIQDVNRLIKQFAEMQKLMKQMSGAGGRRALMSSLGRRR"
     gene            1598..3421
                     /locus_tag="VMZ33_01165"
     CDS             1598..3421
                     /locus_tag="VMZ33_01165"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HUR15868.1"
                     /translation="MTAPRDDPPHSPPAFPSVGGSPIAGHEGHESALPFSPADEVVLA
                     PVATTGARSPNDRWRWVAAGLATILVVALIGGVFLALRPQAGTPSLVAMYAPGDAAAY
                     LEGRLDMPGDQRDKLATFMGRFPGFADPASFQQKIDESLEQALASSQTGLSWTNDIDP
                     WFGGQVGYWMSTINPAPGTPPAFTVAFSVEDRPLLDQLLATKINGSDMQHEEYKGFTI
                     WTGNATDEARRLSLVVTDDAFLISGRNEDLKRSLDVKANEVSGLADDEFFLQQLGLLH
                     ADRLGVFYYDPSTTLTAMPQGMSGLPPGCLNQLNAMSDVKMLGEVRAEGNYMAVNMRM
                     RYPTGEGVPPAPSNKRSPLLDVMPANTVAYFEARQVGRVLQTTISQVLGCMDTGQTPM
                     NLTQIEQALGVSFESYFDFIDDTGVALTYGDGKWGGGLVATVDDEAVAKTRLERLLSF
                     LRMMLGTGGGQGGPTLEEVAHGEVTITTIRIPAAPALPAVPTGPADPSSFGIAVANGR
                     LYMGTDDFVANALDRAASDSLSSSARLESALASAGQENAGIVYVDLAALRGAVESMIP
                     AAQQVNYELEGKPFVEPLDQLIVVTRTDGSILSTHAFLYVE"
     gene            3464..3733
                     /gene="rpsP"
                     /locus_tag="VMZ33_01170"
     CDS             3464..3733
                     /gene="rpsP"
                     /locus_tag="VMZ33_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HUR15869.1"
                     /translation="MAVRIRLTRVGAKKQPTYRFVVSDSRNARDGRSLDTLGHYNPRT
                     DPIEINVDEAKARDWMSRGAKPSETVERLFRRVGILPEADSKAKA"
     gene            3736..>3915
                     /locus_tag="VMZ33_01175"
     CDS             3736..>3915
                     /locus_tag="VMZ33_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HUR15870.1"
                     /translation="MSAESLVEFVAKSLVDEPDEVSVKVVDDSEGRVIELHVAESDMG
                     KVIGRNGSVAKALRTL"
BASE COUNT          736 a         1281 c         1279 g          619 t
ORIGIN      
        1 cggcagggcg actcgagtca cgagggagcc aaagatggaa accgtcgccg cggcccagac
       61 gcgctggaac gcgctgttgc gccacaagga gggcgtgggc aactcgtccg caggcggaaa
      121 atgtgggtcg cggagcggct cgtcggccat cgccggaggg tacgagacgg agcggcacta
      181 aacttgatgt tgcgtcgaga ccagccgagg aaccccaccg aattgtttga cgacctttcc
      241 gatcgcctgc gcggcgtcct gaacaagctg agcggccacg gccgcgtaac tgaagtggac
      301 gtcgatgccg ccatgcgcga ggttcgcctc gccctgctcg aggcggacgt taattacaag
      361 gtcgtcaagg actttgtggc acgggttcgc gagcgggccg tgggcaccga ggtcctcgac
      421 agcctcaacg ccggccagca agtaatcaag atcgtccacg aggagctgac ggcaatactc
      481 ggcgccggtg accgcacctt ccgactggcg ggctcgccgg cggtagtaat gctggtcggc
      541 ctccagggct caggcaagac cacgaccgcg gccaagctga cgcgcgtcgt caccaagcag
      601 ggccggcggc cgctcctcgt ggcggctgac ccgtatcgac ccgcagccgc cgaccagctc
      661 caggcgctcg gccgccagct cgacgtcccc gtgcaccgcg gcccgcccaa tgagaaggtt
      721 ttggacacag tccgcaacgg tctcgccgtc gcgcgtcaaa ccggccgcga cgtcgtgatc
      781 gtggacaccg ccggccgcac gtccgtggac gaggcactcc tcgaagagat ccgcgccctg
      841 gccgctgcga cgaaccccag cgaaacgctg ctcgtcgtcg acgcgatggt tggccaggaa
      901 tcggtgtccg ttgctcaggc cttcgttggc gtggcgccgg tgaccggcct tgtcctgacc
      961 aagatcgatg gcgatgcccg cggtggggcc gcactctcga tcagcgccgt caccgggttg
     1021 cccgtcaagt tcatggggac cggcgagaaa accgacgcac tggagccgtt ccacgccgac
     1081 cgcctggcag ggcgcatcct gggcatgggc gacgtgctga cactggtcga acgcgctcag
     1141 gagacattcg acgagaagca ggccgaggag atggcccgca agctccagcg caaccagatg
     1201 acgctggagg acttcctgtc gcaaatgcag cagctcaagc ggatggggtc gatcggcggg
     1261 ctgctcgaga tgatcccggg catgggcggc gcggccaagg aggcccagca ggccgttgat
     1321 cgcggggacc tgaagcgtgt ggaagcaatc atccagtcga tgacgccacg tgagcgccgc
     1381 gaacctgaca tcctgagctc cagccggcgt aagcgcgtgg ctcgcggctc ggggacgacg
     1441 atccaggacg tcaacaggct gatcaaacag ttcgccgaga tgcagaagct catgaagcag
     1501 atgagcggcg ctggtggccg tcgcgcgctg atgtcgtcac tcggccgccg tcgttgatcg
     1561 tcacacccat ggaacgtcac acagagggga ttgagaaatg accgcgccgc gagacgaccc
     1621 gccgcacagc ccgcccgcct ttccgtcggt aggcggctcg cccatcgccg gccacgaggg
     1681 tcacgaatcg gccctgcctt tcagtcccgc ggatgaagtc gtgctcgcac cagtggcaac
     1741 cacaggcgcg cgatccccga atgaccgctg gcgctgggtg gcagcgggac tcgcaaccat
     1801 cctggttgtc gccctcatcg gcggcgtctt cctcgcgctg cgcccgcagg caggcacgcc
     1861 gtcgctcgtg gccatgtatg cgccgggcga cgcggcggcg tacctcgaag ggcgtctcga
     1921 catgcccgga gatcaacgcg acaagctggc caccttcatg ggccgcttcc cggggtttgc
     1981 cgatcccgcg agcttccagc aaaaaatcga tgagagcctc gaacaagctc tggcgtcgag
     2041 ccagaccggg ctgtcgtgga cgaacgacat cgatccgtgg ttcggcgggc aggttggtta
     2101 ctggatgtca acgatcaatc cagcgccagg tacgccgcct gctttcaccg tcgccttctc
     2161 cgtagaggac cgaccgctcc tcgaccagct gctggccacg aagatcaacg gctcggacat
     2221 gcagcacgag gaatacaagg gcttcacgat ctggaccggc aacgcgacag acgaagcgcg
     2281 gcgcctcagc ctggtcgtga ccgacgacgc gttcctgatc agcggccgca acgaagatct
     2341 gaaacggtcg ttggacgtga aggccaacga ggtcagcggg cttgccgacg acgagttctt
     2401 cctgcagcag ctcgggttac tccacgccga ccggctgggc gtcttctact acgacccctc
     2461 gaccacgctg accgcgatgc cgcagggtat gtccgggctg ccaccgggct gcctgaacca
     2521 gctgaacgcc atgtccgacg tcaagatgct gggcgaggtt cgggccgagg gcaactacat
     2581 ggccgtcaac atgcgcatgc ggtacccaac cggggaggga gttccgccgg cgccgtccaa
     2641 caagcgctcg ccgctcctcg acgtcatgcc tgccaatacg gtggcctatt tcgaggcccg
     2701 gcaggtcggt cgcgtcctcc agacgacgat ctcgcaggtc ctgggctgca tggacaccgg
     2761 ccagacgccg atgaacctga cccagatcga gcaggcattg ggcgtttcgt ttgagagcta
     2821 cttcgacttc atcgacgaca ccggcgtggc cctgacgtac ggtgacggca agtggggcgg
     2881 tggcttggtg gcaaccgttg acgacgaggc agtcgcgaag acacgactcg agcgcctgct
     2941 gagcttcctc cggatgatgc tgggaacagg gggcggccaa ggtgggccga ccctcgagga
     3001 ggtcgcgcac ggcgaggtca ccatcacaac gatccgcatc ccggccgcac ctgccttacc
     3061 agcggtgccg accgggccgg ccgacccatc gagcttcggt atcgccgtcg cgaacggccg
     3121 cctgtacatg ggcacggacg actttgtggc caacgcgctc gatcgggcag cgagcgattc
     3181 gctgtcctca tcggcccgtc tcgaaagcgc gctcgcatcc gctggacagg aaaacgccgg
     3241 tatcgtctac gtcgatctgg ctgcgttgcg aggcgccgtt gaatcgatga tccccgccgc
     3301 gcagcaggtc aactacgagc tcgagggaaa gcccttcgtc gagccccttg atcagctcat
     3361 cgtggtcacc cggacggatg gctcgatctt gtcgacccac gcatttctct atgtcgagta
     3421 acctttccgc cgcaatcaag aagtgaggtt cgtacaaact caagtggcag tccgcatccg
     3481 tttgacgcgc gtcggcgcga agaaacagcc cacgtatcgc ttcgtggtct cagatagcag
     3541 aaacgcgcgc gatggccgat cactcgacac gctcggccac tacaacccgc gcacagatcc
     3601 catcgagatc aacgtcgacg aggccaaggc ccgcgactgg atgagccgcg gcgccaagcc
     3661 gtcggagact gtcgagcggc tgtttcgccg tgtcggcatc ctgcctgagg cagactccaa
     3721 ggcgaaggca tagcgatgtc agccgaatca ctggtcgagt tcgttgccaa atccctcgtc
     3781 gacgagccgg atgaggtgtc ggtcaaggtg gtcgacgact ccgaaggccg cgtgattgag
     3841 ctccacgtcg cagagagcga tatgggcaag gtcatcgggc gcaacggcag cgttgccaag
     3901 gccctgcgca ccctg
//
LOCUS       DATLCO010000042         2469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5986121, whole genome shotgun sequence.
ACCESSION   DATLCO010000042 DATLCO010000000
VERSION     DATLCO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5986121"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VMZ33_01180"
     CDS             complement(<1..810)
                     /locus_tag="VMZ33_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HUR15871.1"
                     /translation="MVQAADQLATPPERPAAGTIRVLMIGDIIGKPGREAVEATLSLL
                     REERHIDLVTANGENIAGGMGLTVSTSQALFRAGVDVITSGNHIWDKREIYAELDREE
                     RILRPLNYGLDGVPGRGWGIYHAADGSEVAVINAQGRTYMPQIDNPFTMLDTLLDDGA
                     DELPPVRIVDFHCELTSEKNAFGIHLDGRVSAVCGTHTHVATADERILPRGTAYLTDI
                     GMTGPVYSVIGFEPTTVLPRFMNAMPTRFEVGSGPVLFNAVQIDVETATGKA"
     gene            complement(849..2381)
                     /gene="rny"
                     /locus_tag="VMZ33_01185"
     CDS             complement(849..2381)
                     /gene="rny"
                     /locus_tag="VMZ33_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HUR15872.1"
                     /translation="MDNPVVLAVVVLVAIAAGFGIGFVVRSLWTSQQMRTATEKAGRI
                     VAEARAQQKELILQAKDDQVRIAREAEEDARTRRAELGNLERRLLQRDEQLDQRADML
                     EERDRKLIGKERELDTLKEELATARQDQIQALERVSQLSQDEAKQLLIEQVRREAEND
                     AVRLARAIERKAKEEAEEKARDVVVTAIQRVMVDHTAEMTVSAVQLPSDEMKGRIIGR
                     EGRNIRALEQATGIDLIIDDTPETVLISGFDPVRREIARLSLTKLIQDGRIHPGRIEE
                     VVAKAKAEVELIMRQAGEQAAYETGVPGLPPEIIKLLGRLKFRTSYGQNVLQHSVETA
                     RLAATIAAEVGADVQIAKMGGLLHDLGKAVDHELEGPHAALGAQIAARHNLPMRVING
                     IAAHHQEVEYACVESPIVQIADAISASRPGARGESSETYLKRLEELQSIANSFEGVEK
                     SFAVQAGREVRILVRPEEIDDLASMRLARDIVRKIEETLTYPGQIKVTVIRETRAVEY
                     AK"
BASE COUNT          377 a          805 c          790 g          497 t
ORIGIN      
        1 ggctttgccc gtggcggtct cgacgtcaat ctgcacggcg ttgaacagca ctggacccga
       61 acccacctcg aagcgcgtgg gcatggcgtt catgaagcgc ggcagaacgg ttgtcggctc
      121 aaagccgatc acgctgtaaa cgggcccggt catgccgatg tcagtcagat aagcggttcc
      181 gcgcggcagg atccgttcgt cggccgtggc cacgtgcgta tgcgtgccgc agacagcgct
      241 gacacggccg tcgaggtgga tgccgaacgc attcttctcg ctggtgagct cgcaatggaa
      301 gtccacgatg cgcaccggcg gcagctcgtc ggcgccgtcg tcaaggagtg tgtcgagcat
      361 cgtgaatgga ttgtcgatct gtggcatata agtgcgcccc tgcgcgttga tcaccgctac
      421 ctctgatcca tcggcggcgt gatagatacc ccagccacga ccgggtacgc cgtcgagccc
      481 gtagttgagg ggccgaagta tgcgctcctc gcggtccagc tcggcataga tctcgcgctt
      541 gtcccagatg tggttgccgc tggtgatgac atcgacgccc gcgcgaaaca gcgcctgcga
      601 cgtgctcacg gtcaggccca tgccgccggc gatgttctcg ccattggccg tgaccaggtc
      661 gatgtgccgc tcttcgcgga gcaaggacag ggtcgcctcg accgcctcgc ggccgggctt
      721 gccgatgatg tccccgatca tcagcacccg gatcgtcccc gcggccggcc gctcaggggg
      781 cgtggctagt tggtccgctg cctggaccat cccccgcgcg gttgcgttct gctccgaacc
      841 ctcgagagct actttgcgta ctccactgcg cgcgtctcgc ggatcacggt gaccttgatc
      901 tgaccgggat aggtcagggt ctcctcgatc ttgcgcacga tgtcacgcgc taggcgcatc
      961 gaggccagat cgtcgatctc ctcggggcgc acaaggatgc gaacctctcg tccagcctgg
     1021 acggcaaatg acttctcaac gccttcgaac gagttggcga tcgactggag ctcctccagc
     1081 cgcttgaggt aggtctctga cgactcgcca cgtgcgcccg gccgtgacgc cgagatggcg
     1141 tcggcgatct ggacgatcgg cgactcgacg caggcgtact cgacctcctg gtggtgcgcg
     1201 gcaatgccgt tgatgacccg catcgggagg ttgtggcgag ctgcgatctg ggcgcccagt
     1261 gcagcgtgtg gaccttccag ctcgtggtcg accgccttgc ccaggtcgtg cagcagacca
     1321 cccatcttgg cgatctgcac gtctgcaccg acctcagccg caatggttgc cgcgaggcgt
     1381 gccgtctcga cggagtgctg aagcacgttc tggccgtagc tggtcctgaa tttgagacgt
     1441 cccagcagtt tgatgatctc cggagggagg ccgggcacgc ctgtctcgta cgccgcctgc
     1501 tcccctgcct ggcgcatgat cagctcgacc tctgccttgg ccttggcgac gacctcctcg
     1561 atgcgtccgg gatggatgcg cccgtcctgg atgagcttcg tcaggctgag tctggcgatc
     1621 tcgcggcgaa cggggtcgaa gccgctgatg agcaccgtct cgggggtgtc gtcgatgatc
     1681 aggtcgatcc cggtggcctg ctcgagggca cggatgttcc gaccttcgcg gccgatgatg
     1741 cggcccttca tctcatcgct tggcagctgg acggcgctga ccgtcatctc cgcggtgtgg
     1801 tcgaccatga cgcgttggat cgcggtgaca acaacgtcgc gtgccttttc ctcggcctct
     1861 tccttggcct tgcgctcaat ggctctagcc agtcggacag cgtcgttctc cgcctcacgg
     1921 cggacttgct cgatcaacag ctgcttggcc tcgtcctgcg agagctggct gacccgctcc
     1981 aacgcctgga tctgatcctg gcgggcggtg gcgagctcct ccttgagagt gtcaagctcg
     2041 cgctccttcc cgatcagctt gcggtcgcgc tcctcaagca tgtcggcacg ctggtcgagc
     2101 tgttcatcac gttggagcag gcggcgctcg agattcccga gctcggcgcg cctggtgcga
     2161 gcgtcctctt cggcctcgcg cgcgatgcgg acctggtcat ccttggcctg gaggatgagc
     2221 tctttctgct gagcacgagc ctcagcgacg atgcgtccgg ccttctcagt cgcagtgcgc
     2281 atctgttgcg acgtccacag ggaacgcaca acgaacccga taccgaatcc ggcagcgatg
     2341 gcgacgagca ccaccaccgc caggacgacg gggttgtcca tatgtatctg cctcccatcc
     2401 ggcccctaac cggaaggatg tatgcaggtt tcgtgggtcg gtgagtggtc gctcatcccg
     2461 agcattccc
//
LOCUS       DATLCO010000043         3964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1539540, whole genome shotgun sequence.
ACCESSION   DATLCO010000043 DATLCO010000000
VERSION     DATLCO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539540"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VMZ33_01190"
     CDS             <1..528
                     /locus_tag="VMZ33_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15873.1"
                     /translation="FSGVGVYGESSTVGFTLDVGTAATYGISGSALGMSPEGEELLTH
                     VPSDWQNQCIQAPAIENEVAAIVCFLQQDGAGVELASYESFGTNEEMDAAYQERVTNF
                     GVAATGSCQDGPNETTWSMEGEGTLGRVQCAPQLAGIRADWTDDRLGILSSLIDFEGN
                     YGLMYETWKNAGPNP"
     gene            739..1416
                     /gene="infC"
                     /locus_tag="VMZ33_01195"
     CDS             739..1416
                     /gene="infC"
                     /locus_tag="VMZ33_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HUR15874.1"
                     /translation="MNEMIRVPQVRVVDEEGNQMGVLATPEALRLAQDRGYDLVEVAP
                     MAVPPVTRLLDYGQFKYEQARREKEARRHQRSVDFKEIRLKVKIGKGDFDTKVRRSIE
                     FLEDGDRVKVTVQFRGREVSHAYLGRDLLLRFAEQVGEHAQVERQPLLEGKQMSLVMA
                     STHKPKIPQPQPNQRDHERDSANGAETDEPVESTPSEEPVETPAPKPRAAKAALTEAA
                     NPAPGAN"
     gene            1509..1715
                     /gene="rpmI"
                     /locus_tag="VMZ33_01200"
     CDS             1509..1715
                     /gene="rpmI"
                     /locus_tag="VMZ33_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HUR15875.1"
                     /translation="MPKIKTHKGAQKRFRVTGGGKLVRVKAWSGHHLDIKSSRRTRRY
                     AGVAVVSETSAEQVKRMLPYLRAK"
     gene            1723..2073
                     /gene="rplT"
                     /locus_tag="VMZ33_01205"
     CDS             1723..2073
                     /gene="rplT"
                     /locus_tag="VMZ33_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HUR15876.1"
                     /translation="MARVKRGVTAHKRHKRLLNAAEGRRGTRSKLIKPAREAQLHAMA
                     YAYVGRKQRKRQMRALWIVRLNAAARQHGLTYGQLINGLKSAGVGLDRKILADIAVRD
                     AATFASIAEVARAR"
     gene            2087..3130
                     /gene="pheS"
                     /locus_tag="VMZ33_01210"
     CDS             2087..3130
                     /gene="pheS"
                     /locus_tag="VMZ33_01210"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HUR15877.1"
                     /translation="MDLASFSAQLEDLRERALAAVAAADDPRELDDVESSYLGRNGEL
                     RGLLGGIGKMPVEDRPRVGALANPVREALEHGIAARRRRLNEIALDVRLAREKTDVTL
                     PGRSLPAGSLHPLQETIRAIARVFAQFGFVVYESPEIETDELNFQLLNIPADHPARDL
                     WDTLYVDGTDRRYLLRTHTSPGQIRLMRESAPPIRAILPGRCYRYEAVDASHGFEFFQ
                     VEGLMIDEGTSMATLRALLDEFAHAMFGAATRTRFRPGYYPFTEPSVAFDISCLICGG
                     VGCAACKRSGWLTILGAGMVHPVVLRNCGLDPDEHQGFAFGMGVDRIAMLRHGIPDIR
                     AFLENDMRFLRAF"
     gene            3133..>3964
                     /locus_tag="VMZ33_01215"
     CDS             3133..>3964
                     /locus_tag="VMZ33_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HUR15878.1"
                     /translation="MRVPLSWLRDYVDFDLPPERLAERLTILGMEVQGIERIGADWSS
                     IVVGELLEVVPHPNADRLSLTKVRVSDTDAPLSVVCGATNIAVGQRVPVALPGAVLPG
                     GKHIGVSTIQGTPSQGMLCSGSELGLTADSDGILILADGLGDGAPRLGTSLEDVVGDV
                     VLDIDVKPNRGDALSMIGLAREVAAISGTHVKWPPIEVPESGDSTDDHLWVEIADKRL
                     CPRFVGRYVDNLQIGKSPVRVQLRLSAAGMRPVSNAVDASNYVMLEMGKPIHVFDAGS
                     V"
BASE COUNT          722 a         1273 c         1312 g          657 t
ORIGIN      
        1 ttctcgggcg ttggtgtcta cggcgagtcc tcgacggtcg gcttcacgct cgacgtcggc
       61 accgcggcta cctacgggat ttccggcagc gcccttggca tgtcgcccga gggcgaagag
      121 ctcctcaccc acgtgccgtc cgactggcag aaccaatgca ttcaggcgcc agccatcgag
      181 aacgaggtgg ccgcgatcgt gtgcttcctt cagcaggatg gcgctggtgt cgagttggca
      241 tcgtacgagt ccttcgggac caacgaggaa atggacgccg cataccagga acgtgtcacg
      301 aactttggcg tcgctgcaac cggctcctgc caggacggcc caaacgagac gacgtggagc
      361 atggagggcg aaggcacact cgggcgcgtg caatgcgcac ctcagctggc cggtatccgg
      421 gcggattgga ccgacgatcg actcggaatc ctgtcttcac tcattgactt cgagggcaac
      481 tacggcctga tgtacgagac ctggaagaac gcgggaccca atccgtagag tcccgacgcg
      541 gggcggatcg ccagatggca ggcccggagc aaccgtcgcc gggccacgcg ctgggaacct
      601 tccccgcgct gagaatcagg gcgcctgtgc tatattcccg gccggccgaa gaggtgtccg
      661 gcgtcgtttg ccgtgcgtcc atgtggccaa aacgggtagg taaagtttga gggatcaggc
      721 ctgagtcgcg agctccgaat caatgagatg atccgcgtcc cgcaggtgcg ggtcgtggac
      781 gaagagggca accagatggg cgtgctggcc acgccggagg ctctccgact cgcgcaggac
      841 cgcggctacg acctcgtgga ggtcgccccc atggctgtcc cgcccgtaac ccgcctgctc
      901 gactacggcc agttcaagta cgagcaggca cgtcgtgaga aagaggcccg acggcatcag
      961 cggtcggtcg atttcaaaga gataaggcta aaagtaaaga tcggcaaggg tgacttcgac
     1021 accaaggtcc gccgctcgat cgagttcctt gaggacggcg accgggtcaa ggtgacggtc
     1081 cagttccgtg gacgcgaggt gagccatgca tacctgggtc gcgatctgct gcttcgcttt
     1141 gcggagcagg tcggagagca cgcccaggta gagcgtcagc cgctgctgga aggcaagcag
     1201 atgtcgctcg tcatggcgtc gactcacaag ccaaagatcc cgcagccgca gcccaaccag
     1261 agggaccacg agcgcgacag cgcgaacggt gccgaaacag acgagccggt cgagtcgacg
     1321 ccgagcgaag agccggttga gacgccagcg ccaaagccgc gggccgcaaa ggcagccctg
     1381 acagaggcag caaacccggc cccaggagcg aattgaccgc aagccaaagg taagcaggcc
     1441 actaaagtgg cctgattgcg gaggcgcgca gcagagcccc gatgggctac tgcggaggaa
     1501 gaagagttat gccgaagatc aagacgcaca agggagccca gaagcgcttc cgcgtgaccg
     1561 gtggcgggaa gctggtccgc gtgaaggcgt ggagcggcca ccacctggac atcaagtcat
     1621 cgcgtcgcac gcggcgttac gccggggtgg cagtagtgag cgagaccagc gccgagcagg
     1681 tcaagcggat gctgccctac ctgcgcgcga agtaggtacg taatggcacg agtcaagcgc
     1741 ggcgtcaccg cgcacaagcg ccacaagcgc ctcctgaacg cggccgaggg tcggcgcggg
     1801 acacgttcga agctcatcaa gccggcgcgt gaggctcagc ttcatgcgat ggcatatgcg
     1861 tatgtcggcc gcaagcagcg gaagcgccag atgcgtgccc tgtggatcgt ccggctcaac
     1921 gccgccgccc gccagcatgg cctcacatac ggtcagctga tcaacgggtt gaaatcggcg
     1981 ggcgtgggcc tcgatcgcaa gatactggcc gacatcgcgg ttcgcgatgc ggccacgttt
     2041 gccagcatcg ccgaggtcgc tcgagccagg tagcggcagt caagccgtgg acctggcgtc
     2101 cttctccgct cagctagagg acctgcgcga gcgtgcgctc gccgcggtcg ccgctgctga
     2161 cgatccccgg gagctcgacg acgtcgagtc gtcctacctt gggcgtaacg gtgagctgcg
     2221 cgggcttctc ggcggcatcg gcaagatgcc cgttgaggat cgcccgaggg tgggcgccct
     2281 ggcgaatcct gttcgggagg ccctcgaaca cgggatagcg gcacgtcgcc ggcgtctgaa
     2341 tgagatcgca ctggatgtgc ggctcgcgcg cgagaagacg gacgtcacgt tgcccggccg
     2401 gtcgctaccc gccggttcgc tccatccact ccaggaaacc atccgcgcga tcgcacgcgt
     2461 cttcgcccag ttcgggttcg tcgtctatga gtcgcccgag attgagaccg acgagctcaa
     2521 cttccagctt ctgaacatcc ctgcagatca cccggcgcgt gacctctggg acacgctcta
     2581 cgttgatggc acggaccgcc ggtacctgct ccgtactcac acgtcacccg gccagattcg
     2641 actgatgcgc gagtccgcgc caccgattcg agccatcctg cccggacgtt gctatcgcta
     2701 cgaggcggtc gatgccagcc acggcttcga gttcttccag gtcgagggcc tgatgatcga
     2761 cgagggcacg tcgatggcga cgctgcgcgc gcttctggac gagtttgccc atgccatgtt
     2821 cggcgccgcg acacggaccc gtttccgccc gggctattac ccgttcaccg aaccttcagt
     2881 cgctttcgac atcagctgcc tgatctgcgg cggcgtcggc tgcgccgcct gcaagcggag
     2941 cggctggctg accatcctcg gagcgggaat ggtccacccc gtcgtgctcc gcaactgcgg
     3001 actggatccg gacgaacacc agggcttcgc cttcggcatg ggagttgatc ggatcgccat
     3061 gctgcgccac ggaattcccg acatccgcgc attcctcgag aacgacatgc gcttcctcag
     3121 agctttctag ccatgcgagt gccgctttcg tggttgcgcg actacgtcga cttcgacctg
     3181 ccacccgaac gcctggctga gcgcctgacg atcctgggca tggaggtcca ggggatcgag
     3241 cggatcggcg cggactggtc ctcgatcgtt gtcggtgagc tgctcgaagt tgtgccgcac
     3301 cccaatgccg accgcctgtc gctgaccaag gtgcgcgtct cggacaccga cgcgccgctt
     3361 tccgtcgtgt gcggggcgac caacatcgcg gtcggccagc gcgtacccgt ggcactcccc
     3421 ggcgccgtgc tgccgggcgg caagcacatc ggcgtctcca cgatccaggg cacgcccagc
     3481 cagggcatgc tgtgctctgg ttcggagctc ggactcacgg ccgattcaga cggcatcctg
     3541 atccttgcgg acggccttgg tgacggcgcg ccgcgtctcg gcacctcgct cgaggatgtc
     3601 gtgggcgacg tcgtcctgga cattgacgta aagcccaacc gcggcgacgc gctgtcgatg
     3661 atcggccttg cccgcgaggt cgccgctatt tccggcacgc acgtgaagtg gccaccgatc
     3721 gaggtgcccg agtccgggga ttccaccgac gaccacctct gggttgagat cgccgacaag
     3781 cggctgtgcc cgaggttcgt gggccgctac gtcgacaacc tgcagatcgg caaatcgccg
     3841 gtgcgcgttc agttgcggct gtccgctgcg gggatgcggc ccgtctcgaa cgcagtagat
     3901 gcctcgaact acgtcatgct cgagatgggc aagccgatcc acgtattcga cgctgggtcg
     3961 gtga
//
LOCUS       DATLCO010000044         4761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7477772, whole genome shotgun sequence.
ACCESSION   DATLCO010000044 DATLCO010000000
VERSION     DATLCO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7477772"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..2164
                     /locus_tag="VMZ33_01220"
     CDS             290..2164
                     /locus_tag="VMZ33_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15879.1"
                     /translation="MVALIFNNQLDIQVDSQNELFFGLAESRAADQNFLILPLIVSLV
                     VGLLATLALPLGPLLKSMPPLRAYAIDIGGSMAGIALFAVLSAFGTSPPLWFAIAVAL
                     VVVLDIGRGVSMWTAANIAVLAAVLLGVIGHAQARPNEIWSPYYRISSFKASSGLEHL
                     TVDGIPHQALHPLGEENIEEFYEQIYRWFPGKTYEEVLIVGAGSGSDVAIALSHGAGH
                     VDAVEIDPKLLEIGKTRHPNHPYDDPRVTPYNNDGRAFLRTSDKDYDLVIFALPDSLT
                     LVSQQSSLRLESFLFTEQAFATVRDHLTDDGVFVLYNYYRDPWLVAKLGTMLEDAFGH
                     PPLASTYGAVKATLASGPLIAGLPDGVPPGQAPSGLPAAGEPEPTPATDNWPFLYLRN
                     PFIPVHYLAAIAIVLIGAVVAVAAAARVTGTTIRRFSPHFFVLGVAFLLLETRSLTSF
                     ALLFGTTWLVNALAFFAILLSVLLAIFVNSRWPIRRPTLYYVALFIAVAVAFLLPPES
                     LLFDPPWLRYVVAGALAFAPVFLANLVFSYSFRDTNTADMAFASNLLGAMVGGMLEYM
                     ALVTGYQALLLVVAVLYALAWLFATRFRFGSDKELDAPDRMVDVSPIPPQPITPGAPA
                     "
     gene            complement(2165..2710)
                     /locus_tag="VMZ33_01225"
     CDS             complement(2165..2710)
                     /locus_tag="VMZ33_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15880.1"
                     /translation="MAVFGCGGGATATPATTSGQPASAIPSVGGAPVPPPAGGESASV
                     TLTGGDDAGSYTASGEPNCTNGLLGTGTWGVQFSLAEAAADQLSSVQLVYRSDGTGGG
                     DDDMFGGVSTLFTVAFGSLMEPEYRDYEVEVRTDGEESSGTGTVTVTDNGSTAVIHAV
                     GTTAEGVGIDATVNCPSVMRM"
     gene            2918..>4761
                     /locus_tag="VMZ33_01230"
     CDS             2918..>4761
                     /locus_tag="VMZ33_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR15881.1"
                     /translation="MPKERRGRTIRRIAAFFKPYRLAVLAVLLTIVVTSFLGLINPYL
                     LKLLIDEALPQRDLGLLNLFVGLMIVVPIVSGLIGVGQSYLNNIIGQRVMQDLRNALY
                     AHLQKLPLRFFTETRTGEIQSRLANDVGGVQSVVTDTASSVIANLVVVLSTVGAMLIL
                     DWRLTALSLAMLPFFLYLTYRVGKVRRRLSGMTQVSLADISAITEETVSVSGMLLTKT
                     FGRQQGAIDRFRDANAKLAALQIRQSMVGRWFFMIVGTIFSVTPALVYWLAGWLAIQG
                     DPTAPTVGDIVAFTTLQSRLFFPLGQLLNVQVEVQGALALFDRIFEYMDLAPEIEDAP
                     NAVALQRDSVRGRIRFRHVSFRYRGKETAPEPAVPTNGGEPGGGGESVGGRESVGGGE
                     PEPKPIPEPERLPFGVEDIDFEAEPGQLIALVGPSGSGKTTTTYLLPRLYDVDGGAVE
                     IDGVDVRQIKLSSLGEVIGFVTQETFLFHTSVRENLRYAKPEATDHELEVAARLAAIH
                     DRVRELPDGYDTIVGERGYKLSGGEKQRVALARVLLKDPRILVLDEATSALDTVSERL
                     IQAAIERAMKGRTTIAIAHRLSTILRADQILVLDRGRVIERGRHDELL"
BASE COUNT          746 a         1672 c         1467 g          876 t
ORIGIN      
        1 ggcactttcg gcgggcgcgg ataccatgcg gccaattcat ccctgactac tggaggacgg
       61 tctcctgcgg ttcctggcgc gcaatgaggt ccgcctcttt ctgaccagcc tggcgatcct
      121 gttcgtcgag cttctgctca ttcgttggat cccggcgaac gtcaagtaca tcggcttctt
      181 cagcaacttc ctgctgatcg ccagcttcct gggcatcggc ctggggatcc tgctcggaag
      241 acgcggcgcc aacccaccca tcgggccctt cgcgttcctc ctggcggccg tggttgcgct
      301 gatcttcaac aaccagctcg acatccaggt cgactcgcag aacgagctct tcttcggtct
      361 ggccgagagc cgcgcggcgg accagaactt cctgatcctg cccttgatcg tgtcgctcgt
      421 cgtcggcttg ctggcgaccc ttgccctgcc actcgggccg ctcctcaaga gcatgccgcc
      481 gctgagggca tacgcgatcg acatcggtgg ctccatggcc ggcatcgcgc tcttcgcggt
      541 gctgtcggcc ttcggcacct cgccgccgct gtggttcgcg attgccgtcg cactcgtggt
      601 cgtgcttgat atcggccgtg gcgtctcgat gtggaccgcc gcaaacatcg ccgtgcttgc
      661 ggcggtgctg ctaggcgtga tcggccacgc ccaggcccgc cccaacgaga tctggtcgcc
      721 gtattaccgg atcagctcgt tcaaggcgtc cagtggtctc gagcatctca ccgtcgacgg
      781 gatcccccac caggccctgc acccgctcgg tgaggagaac atcgaggagt tctacgagca
      841 gatctatcgc tggttcccgg ggaagaccta cgaggaagtg ctgatcgtgg gcgccggcag
      901 tggctcggac gtcgcaatcg cgctgtccca cggggccggc catgtcgacg cggtggaaat
      961 cgacccgaag ctcctcgaga tcggcaagac gcgccatccg aaccacccgt acgacgaccc
     1021 gcgcgtcacg ccctacaaca acgacgggcg tgcgttcctg cggacctccg acaaggacta
     1081 cgacctggtc atcttcgccc tgcccgactc gctcacgctg gtcagccagc agtcgtcgct
     1141 gcgcctcgag tcgttcctgt tcacagaaca ggccttcgcc actgttcgtg accacctgac
     1201 cgacgacggc gtcttcgtcc tctacaacta ttaccgcgac ccgtggttag ttgcgaagct
     1261 cgggacgatg ctcgaggacg ccttcggcca cccgccactc gccagcacgt acggcgcggt
     1321 caaggccacg ctcgccagtg ggccgctcat cgccgggctc cccgacggcg tgccaccagg
     1381 tcaagcgccc agcggactgc cagcggccgg cgagcccgaa cccacgcccg ccaccgacaa
     1441 ctggccgttc ctgtacctgc gcaacccgtt catccccgtc cactacctcg cggcgatcgc
     1501 gatcgtgctc atcggcgccg tggttgccgt ggcggctgct gcccgcgtga caggcacgac
     1561 gatccgccgc ttcagcccgc acttcttcgt gctgggcgtg gccttcctgt tgctggagac
     1621 gcgcagcctg acctcgttcg cgctgctctt cggcacgaca tggctggtca acgcgctggc
     1681 tttcttcgcc atcctgttga gcgtcctgct ggcgatcttc gtcaactcgc gctggcccat
     1741 ccgccgtccg acgctctact acgtcgccct attcatcgcg gttgccgtgg cgttcctgtt
     1801 accgcccgag tcactgctct tcgacccacc gtggctgcgc tacgtcgtcg ccggtgcgct
     1861 cgcgttcgca ccagtcttcc tggcgaatct cgtgttcagc tattcgttcc gcgacaccaa
     1921 caccgccgac atggcattcg ccagcaacct tctgggcgcg atggtcgggg gcatgctcga
     1981 gtacatggcg ctcgtcacgg gttaccaggc cctgttgctg gtcgtggccg tgctctacgc
     2041 cctggcctgg ctcttcgcga cgcgcttccg cttcggatcc gacaaggagc tcgacgcgcc
     2101 ggaccgaatg gtcgacgtca gcccaatccc accccagccg atcacgcccg gggcgcccgc
     2161 ttagctacat ccgcatgacg gaggggcagt tcaccgtcgc gtcgattcca acgccctcag
     2221 ctgtcgtgcc gacggcgtga atgactgcag tggatccgtt atccgtcacc gtgaccgtgc
     2281 cggttcccga cgattcttcg ccgtccgtcc gaacctcgac ctcatagtcg cgatattcgg
     2341 gctccatcag cgacccaaaa gccaccgtaa agagcgtact gactccgccg aacatgtcat
     2401 cgtcaccacc gccggtgccg tcggatcggt agacgagttg gacgctgctg agctgatcgg
     2461 ccgcggcttc cgccaacgaa aactggacgc cccacgttcc agtgcccagc aaaccgttcg
     2521 tgcagttcgg ttcacctgaa gcggtgtagc ttccagcgtc gtcaccgccg gtcaaagtga
     2581 ccgacgcgga ctcgccgccg gccggtggcg gaactggcgc tccgccgacc gacggaatcg
     2641 cgctcgccgg ctgacccgac gtcgtggccg gcgtcgccgt tgcacctccg ccgcagccaa
     2701 acactgccat ggccatcaac cctgcgaata tcacgcgcat gaatccacct ctccgacgaa
     2761 ctgttgcgag aactccccgc ccgggtgaga ccgtatcagc cgcgttttcc cgcgctgtcg
     2821 actcaccagc gctcccccct gcgtcggcgt gagctttcgg ggcggtcgat acggtggtgg
     2881 cgggcatttc ggaatggccg gagatgagca cgccccggtg ccgaaggagc ggcgcggccg
     2941 gaccatccga cggatagccg cgttcttcaa gccctaccgg ctggcggtcc tggccgtcct
     3001 gctgacgatc gtcgtcacga gcttcctcgg gctcatcaac ccgtacctgc tcaagctgct
     3061 tatcgacgag gccctgcccc aacgcgatct aggcctgctc aacctcttcg ttggcttgat
     3121 gatcgtcgtc cccatcgtca gcggcctgat cggcgtgggg cagtcgtacc tcaacaacat
     3181 catcggtcag cgcgtgatgc aggacctgcg caacgccctg tatgcccatc tccagaagct
     3241 gccgctgcgc ttcttcaccg agacgcggac gggcgagatc caaagccggc tggcgaacga
     3301 cgtcggcggc gtccagtcgg ttgtcaccga cacggcgtcg agcgtcatcg cgaacctggt
     3361 cgtggtcctc tcgaccgtcg gtgcgatgct catcctcgac tggcggctca cagcgctgtc
     3421 gctcgcgatg ctgccgttct tcctgtacct gacgtaccgc gtcggcaagg tccgccgccg
     3481 cctctcgggg atgacacagg tgtcgctggc cgacatcagc gcgatcaccg aggagaccgt
     3541 aagcgtcagc ggcatgctgc tgaccaagac gttcggtcgg cagcagggcg ccatcgaccg
     3601 gttccgcgac gcgaatgcca agctcgccgc gctacaaatc cgccagtcca tggtcggccg
     3661 ctggttcttc atgatcgtcg gcacgatctt ctcggtgacg cccgcgctcg tgtactggct
     3721 ggccggctgg ctggcgatcc agggcgaccc gacggcgccg accgtcggcg acatcgtcgc
     3781 gttcacgacg ctccagtcac ggctgttctt cccgttggga cagctgctca acgtgcaggt
     3841 cgaggtgcag ggcgcattgg cgttgttcga ccggatcttc gagtacatgg acctggcgcc
     3901 ggagatcgag gacgcgccaa acgcggttgc gctccaacgt gattcggtgc gtggccgcat
     3961 ccgcttccgc cacgtctcct tccgatatcg cggaaaggag accgcgcctg agcccgcagt
     4021 gccgacgaac ggcggggagc cgggcggcgg cggggagtcc gtcggtggaa gggagtccgt
     4081 cggtggcggg gagcccgaac ccaaaccgat ccccgagccg gagcgcttgc cgttcggcgt
     4141 ggaggacatc gacttcgagg ctgagccggg acagctgatc gcgctggtcg ggccgtcagg
     4201 gtcaggcaag accacgacca cgtacctgtt gccgcggctc tacgacgtcg atgggggtgc
     4261 ggtcgagatc gacggcgtcg atgttcgcca gatcaagctg tcgagcctgg gcgaggtgat
     4321 cggcttcgtg acacaggaga cgttcctctt ccacacgtcg gtgcgcgaga acctgcgcta
     4381 cgccaaacca gaggcgaccg accacgagct cgaggtcgcc gcgcgattgg cggcgatcca
     4441 cgaccgggtg cgcgaactcc ccgacggcta cgacacgatc gtcggcgagc gcggctacaa
     4501 gctctccggt ggcgagaagc aacgcgtggc gttggcacga gtgctgctca aggacccgcg
     4561 gatcctcgtg ctggatgagg ccacttccgc gctcgacacg gtgagcgagc ggctaattca
     4621 agcggccatc gagcgggcga tgaagggccg caccacgatc gccatcgcgc accggctgtc
     4681 cacgatcctg cgcgccgacc agatcctcgt cctcgatcgt ggccgggtga tcgagcgcgg
     4741 tcgccacgac gagctgctcg a
//
LOCUS       DATLCO010000045         3551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3006734, whole genome shotgun sequence.
ACCESSION   DATLCO010000045 DATLCO010000000
VERSION     DATLCO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3006734"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VMZ33_01235"
     CDS             <1..693
                     /locus_tag="VMZ33_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUR15882.1"
                     /translation="WLEFYAARFDTVESNAAFYRLPEAKTFEAWAQRTPPEFVMAVKA
                     SRYLTHIKRLADPEEPVQLLVERASKLGPKLGPILLQLPPTMRINVDRLAATLEAFPK
                     DWRVAVEFRHDSWYIDEVRAVLEEHQAALCLADRWQPLTPIWRTTDWTYVRFHSGRAT
                     PFPCYGRQTLATWVQRLADNWTADEDMYVYFNNDPRACALRDAIVFERLCQRAGLRPT
                     QVPKSREVTLGD"
     gene            complement(690..1916)
                     /locus_tag="VMZ33_01240"
     CDS             complement(690..1916)
                     /locus_tag="VMZ33_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR15883.1"
                     /translation="MGIDLSLTDENRLVQSTVRAFAAQEIEPHIREWDAKHEAQPDVF
                     ARMGSLGFLGAPIPEEYGGNGLDYVSLGILCEELERADTAFRVVMSVHVGLNSLTLLQ
                     WGTEDQKRRFLVPQARGEKLATFGLTEPGVGTDAANLATTAKRDGDSYVLNGQKIWIS
                     LADLADHFLVFATVDRAAKHRGITAFIVERGMEGLSTRTIHGKLGIWAGNTGEIYFDN
                     VRVPAENRVGEEGEGFTIAMSAIDQGRYTVASGAVGLAQACLDASVRYAHERKTFGQE
                     IGEHQLVKQMIANMGAGIEAGRLLCRQVAWLKNRGDRNTRETSLAKWFTTEHATKAAL
                     DAIQVHGAYGYSDEYPVARYLRNSKAAVIYEGTSQLHTLIQADYLLGYREDKPLRCPP
                     LPAPGFERGSDRRVAR"
     gene            complement(1916..3019)
                     /locus_tag="VMZ33_01245"
     CDS             complement(1916..3019)
                     /locus_tag="VMZ33_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HUR15884.1"
                     /translation="MATHNETLSQPTYVAPGPPTDGPLTAEELALAVRNRGMPLEALR
                     YDLTPTGLHYLLIHFDIPATTAQSWRLGIGGAVEAPFAMDMDEIRALPARTLAVTMEC
                     AGNGRARLSPRPMSQPWLYEAIGTAEWTGTSVWPLIERARPRSDVVDYVFTGADRGIQ
                     GEIEHDYARSLTEDELRRGEVMLVYEMNGRPLEPQHGYPLRLIVPGWYGMTSVKWLRS
                     IEAVTTRFEGYQQTPAYHFADDAENLGEAVTRIRPRALMVPPGMPDFFSRHRIVDAGA
                     VALFGRAWSGRSRIERVEVAVDGVWTDAVLGNPLGEFAWRSWTFDWQAETGEHVLSVR
                     ATDATGDVQPTDQPWNYQGMGNNLTQVVPVTVR"
     gene            complement(3134..3328)
                     /locus_tag="VMZ33_01250"
     CDS             complement(3134..3328)
                     /locus_tag="VMZ33_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR15885.1"
                     /translation="MASMGVHEIGPMLTATEVANLLHLHVNTVKRLGDRGELPFYRVC
                     KRGDRRFRLDDVMQFLARNR"
BASE COUNT          581 a         1191 c         1138 g          641 t
ORIGIN      
        1 tggctggagt tctacgccgc gcggttcgac accgtcgagt cgaacgccgc cttctaccga
       61 ctgcctgagg ccaagacatt cgaggcctgg gcgcaacgga cgccgcccga gttcgtgatg
      121 gccgtgaagg ccagccgata cctgacgcac atcaagcgcc tggcggaccc cgaagagccc
      181 gtccaactgc ttgttgaacg ggcgtcaaaa ctgggcccga aactgggccc catccttctc
      241 cagctgccgc cgacgatgcg gatcaacgtc gatcgactcg ctgcgaccct ggaggcgttt
      301 ccgaaggact ggcgggttgc cgtcgaattt cggcacgact cgtggtacat cgacgaggtt
      361 cgggcggtcc tcgaagagca tcaggcggct ctctgcctcg ctgaccgctg gcagccgctc
      421 acgccaatat ggcggacgac cgactggacc tatgtgcgct tccactccgg ccgtgcgacc
      481 ccgttcccct gctacggccg acagaccttg gccacgtggg ttcaacgact ggccgataac
      541 tggacggccg acgaagacat gtacgtgtac ttcaacaacg acccgcgagc gtgcgcactg
      601 agggacgcga tcgtgttcga gcgcctctgc caacgcgcgg gccttcgccc tacgcaggtg
      661 cccaagtcgc gcgaagttac gctcggcgac tagcgggcca ctcgccggtc agaccctcgt
      721 tcgaacccgg gggcgggcag tggtgggcac cggagtggct tgtcctcgcg gtagccgagc
      781 aggtagtcgg cctggatcag cgtgtgcagc tgggacgtgc cctcgtagat gaccgccgcc
      841 tttgagttgc gcaggtagcg ggccaccggg tattcatcgg agtagccata cgccccgtgg
      901 acctgtatcg cgtccagcgc agccttggtc gcgtgctcgg tggtgaacca tttcgcgagc
      961 gaggtctcgc gcgtgttgcg gtcaccgcgg ttcttcagcc acgcgacctg gcggcacagc
     1021 agccgccccg cctctatccc ggcgcccatg ttggcgatca tctgcttgac gagctggtgc
     1081 tcgccaatct cctgtccgaa cgtcttgcgc tcgtgcgcat agcgcacgct cgcatccaag
     1141 cacgcctgcg cgaggccgac cgcgcccgaa gccacggtgt agcggccctg gtcgatcgcg
     1201 ctcatcgcga tcgtgaaccc ctccccctcc tcgccgacgc ggttctcggc cgggacgcgg
     1261 acgttgtcga agtagatctc cccggtgttg cccgcccaga tgccgagctt gccgtggatc
     1321 gtccgcgtgg agaggccctc catcccacgt tcgacgatga acgcggtgat tccgcgatgt
     1381 ttcgcggcgc ggtcaaccgt cgcgaacacc aggaagtggt cggcaaggtc cgccaggctg
     1441 atccagatct tctgtccgtt gaggacgtac gagtcgccgt cgcgcttcgc tgtcgtggcc
     1501 aggttcgcgg cgtctgtgcc gacgcccggc tccgtcagac caaacgtcgc cagcttctcg
     1561 ccgcgcgcct gcgggacgag aaatcgacgc ttctggtcct ccgtgcccca ctgcagcaac
     1621 gtcaacgagt tgagccccac gtggacgctc atcaccaccc ggaacgcggt gtcggcgcgc
     1681 tcgagctcct cgcacaggat gcccaggctc acgtagtcga gcccgttgcc gccgtactcc
     1741 tcggggatgg gcgcgcccag gaacccgagg gagcccatcc gcgcaaagac gtccggctga
     1801 gcctcgtgct tagcgtccca ctccctgatg tgtggctcga tctcctgcgc tgcaaacgcg
     1861 cggacggtcg attggaccag acggttctcg tctgtcagcg acagatcgat gcccatcagc
     1921 gaaccgtgac cgggaccacc tgggtcaagt tgttgcccat gccctggtag ttccaaggct
     1981 ggtcggtcgg ctgcacatca cccgtcgcat cggtcgccct gaccgataag acgtgctcgc
     2041 ctgtttccgc ctgccagtcg aacgtccacg atcgccaggc gaactcccca agtgggttgc
     2101 cgagcaccgc gtccgtccaa acgccatcaa ctgcgacctc gacgcgctcg atgcgcgacc
     2161 tgcccgacca agcccgccca aacagcgcca ctgctcctgc atccactatc cgatgacggc
     2221 tgaagaaatc gggcataccg ggcggcacca tcagggcgcg tggccggatg cgggtgactg
     2281 cctcgcccag gttctcggca tcgtcggcga agtggtaggc cggcgtctgc tggtagccct
     2341 cgaacctggt cgtaacagcc tcgatcgagc gcagccactt cacgctggtc attccgtacc
     2401 aaccgggcac gatcaggcgc agcggatagc cgtgctgggg ctccagcggc cgtccgttca
     2461 tctcgtacac gagcatcacc tcaccgcgcc gcagttcatc ctcggtcagg gaacgggcgt
     2521 agtcgtgctc gatctctccc tggatgccgc gatcggcacc ggtgaacacg tagtcaacga
     2581 cgtccgagcg gggtcgtgcg cgctcgatga gcggccacac cgacgttccg gtccactcgg
     2641 cggtgccgat ggcttcgtat agccacggct ggctcattgg tcgcggcgag agtcgggcac
     2701 gaccgttccc ggcacactcc atggttaccg caagcgtccg ggctgggagc gcacggatct
     2761 cgtccatgtc catcgcgaaa ggcgcctcga ccgcaccgcc gatccccagg cgccaactct
     2821 gagccgtggt cgctgggatg tcgaaatgga tgagcaggta gtgcagcccg gtcggagtca
     2881 ggtcgtagcg cagcgcctcg agcggcattc ccctgttgcg cacagccagc gcgagctcct
     2941 ccgcggtcag cggcccatca gtgggtggac cgggcgcgac gtaggttggc tgggacaggg
     3001 tttcgttgtg cgtcgccatg tgtcggcatc gtaaacgcgc agaaccgccg gcgatatgcc
     3061 ggcggctcat gatcagagat gagtcctcgg cgggcgcgcc ccgtgcccgc cgatgaggga
     3121 agaggctggg ttgttagcgg ttgcgggcga ggaactgcat cacgtcgtca aggcggaagc
     3181 gacgatcgcc acgcttgcag acgcgataga agggcagctc gccacggtcg ccgagccgtt
     3241 tgacggtgtt gacgtgaagg tgcaacaggt tcgcaacctc cgtcgccgtc agcatcggcc
     3301 ctatctcgtg gactcccatt gaagccatgt gtcgtcctct caggtgtttg atcgaccggt
     3361 tgtgtcgtgt gtggtgagtg accgaatgct ccctttcgga gggcccgacc ggaagccgta
     3421 ctttggggac tgaagtacag gtacggaggg tgttgcaggt cggcaacggt cggaaatgtg
     3481 tagtcggctg atatctcgca taaggtgccc cgcgtgacct cgcgcctcgc ctccgtctcg
     3541 gtccgcggcc t
//
LOCUS       DATLCO010000046         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10459045, whole genome shotgun sequence.
ACCESSION   DATLCO010000046 DATLCO010000000
VERSION     DATLCO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10459045"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1772)
                     /locus_tag="VMZ33_01255"
     CDS             complement(186..1772)
                     /locus_tag="VMZ33_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUR15886.1"
                     /translation="MKSGRWHWAGAGAAAGAVAIGVGEITAALLGGTSIIAAVGTLVI
                     ALQPPGGKDLAVQLFGTNDKLALEIGAALGGILVGALLGVFSRKDVRIGVIGFVAFGA
                     FAFYLLQSDPLSGMVPALASAAAATGAGSVMLTWLTTSLRRPAATGTAAVATSAMGEA
                     QLAATPRPSIDRRGFLALSAMFVAVGGVLAFIGRYLTSQVPAVVADPSSIPAARQTVA
                     PVAGATDFGVEGLTPIVVPNDDFYRIDTRLDVPRVDAATWSLRVHGLVDREVTLTYEQ
                     LLAMPLVERYVTIACVSNPVGGRLVGNAKWTGVPLTTVLEQAGVKPEASQLVGRSFDG
                     WTAGFPTEHLRGAGSEALIAVAMNGVPLPPAHGFPARLVVPGLYGYVSATKWITEIEL
                     TTLESFDAYWVPLGWSKIGPILTQSRVELPRAGQQLAAGPVQFAGVAWAPTRGINKVE
                     VIVDPTPEGAGTWHQAELSQPLSSTTWVQWRVTLDVPTGEHRIAVRATDGTGETQTSD
                     ITPPPPDGARGYHQVTFSAR"
     gene            1873..>2259
                     /locus_tag="VMZ33_01260"
     CDS             1873..>2259
                     /locus_tag="VMZ33_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HUR15887.1"
                     /translation="MIRLRFLVLSFALAVTSLAVSASGDYRVALAAGALPACRYDDVM
                     TDHTGYRQWNKTLIDSMFRLPKSYMPPPSLVSTDQANVRGNEKLRQIVIADLKALVRA
                     ARLANVRLMVVSGYRSWAEQKYLYDRE"
BASE COUNT          408 a          768 c          731 g          352 t
ORIGIN      
        1 aagtccatta ccgagctccg gatcaacgat cgcatcgtca tcccgatgcg cggccggcta
       61 cccgtgggct ggttgccgac cgacgccttt gcctcgctcg ctgagcgcga ggcggccatc
      121 gcctacatga gggaccgcat cacggacgca gacacccgca gctaggtggc gaccggcttg
      181 gagggctagc gcgcgctgaa ggtcacctgg tgatagccgc gtgccccgtc gggcggcggt
      241 ggcgtgatgt ccgaggtctg cgtctcgcct gtgccgtccg tggcccgaac ggcgatgcga
      301 tgctccccgg tgggcacgtc cagggtcacc cgccactgaa cccaggtggt tgaggacagg
      361 ggctgtgata gctccgcctg atgccaggtt ccggcgccct cgggagtggg atcgacgatc
      421 acctcgacct tgttgatgcc acgtgtcggt gcccatgcga cgccagcgaa ctggacgggg
      481 cccgccgcga gctgctgacc tgcgcgcggt agctccacgc gcgactgggt cagaatcggc
      541 ccgatcttcg accaaccgag tggtacccag taggcatcaa aagattcgag ggtcgtcagc
      601 tcgatctccg tgatccactt cgtcgccgac acgtagccat aaaggccggg cacgacgagc
      661 cgcgccggga agccgtgcgc cggcggcaac ggcacgccgt tcatcgcgac ggcgatcagc
      721 gcctcgctgc cagcaccgcg caaatgctcc gtcgggaacc cggcggtcca gccatcgaac
      781 gaacggccga cgagctggct tgcctcgggc ttcacaccgg cctgctccag caccgtcgtc
      841 agcggcacgc cagtccactt cgcattgccg accagccggc caccgaccgg gtttgagaca
      901 cacgcgatcg tcacgtaccg ctcgacgagc ggcatcgcca acagttgctc gtacgtcagc
      961 gtcacctccc gatccaccag gccgtggaca cgcaggctcc acgtggccgc gtcgacgcgc
     1021 gggacatcga ggcgcgtatc gatccgatag aaatcgtcgt tcgggacgac gatgggcgtg
     1081 aggccctcca cgccgaaatc cgtggcacca gctaccggcg cgaccgtttg acgcgccgcc
     1141 gggatggagc tgggatcggc gaccacggca ggcacctgcg aggtcagata gcggccgatg
     1201 aaagccagca cgccgccgac ggccacgaac attgcactca gcgcgaggaa gccgcgacgg
     1261 tcgatagaag gtctgggtgt cgcagccagt tgagcctcgc ccatggctga ggtggcgacg
     1321 gcagccgtgc cggtagcagc tggtctccgg agcgaggtcg tcagccaggt cagcatcacc
     1381 gagccggcac ctgttgcggc tgctgcgctc gccagggccg ggaccatgcc cgaaagcgga
     1441 tcgctctgaa gcaggtagaa cgcgaacgcg ccaaatgcga caaagccgat gacgccgatc
     1501 cgaacgtctt ttcgggaaaa cacgcccagc agggcaccga cgaggatgcc gcccagcgcc
     1561 gcccctatct cgagcgccag cttgtcgttt gtaccaaaca gctgaacggc gaggtccttg
     1621 ccgccaggcg gctggagtgc gatcacaagc gtgccgacgg cggcgatgat ggaagtgccg
     1681 ccgaggagcg cggcagtaat ctcgcccacg ccgatcgcaa cggcgcccgc ggcggcgcca
     1741 gcccctgccc agtgccaacg gccggacttc atgacgtcag gttatgcgca cggctcggct
     1801 cgggggatca caccgacgtt ggtcaacttg acggttccgc atctggcccg tcacgattca
     1861 agggccgtga ccgtgatcag actcaggttt ttagtgctgt ccttcgccct ggccgtgaca
     1921 tcgctggctg tcagcgccag cggggattac cgcgtcgcgc ttgccgctgg cgcgctgccc
     1981 gcatgccgat atgacgacgt catgaccgat cacaccggct acaggcaatg gaacaagacg
     2041 ctgatcgact ccatgttccg gctgccgaag tcgtacatgc cgccaccctc tctcgtctcg
     2101 accgaccagg cgaacgtgcg cggcaacgag aagctccgtc aaattgtcat cgccgacctc
     2161 aaggctctcg tccgagccgc gcgcctggcc aacgtgcgtt tgatggtcgt ctccggatat
     2221 cggagctggg cggaacagaa atacctctac gaccgcgag
//
LOCUS       DATLCO010000047         2775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4523520, whole genome shotgun sequence.
ACCESSION   DATLCO010000047 DATLCO010000000
VERSION     DATLCO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4523520"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VMZ33_01265"
     CDS             <1..1198
                     /locus_tag="VMZ33_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR15888.1"
                     /translation="VLRLSVTIFLIQAGFHAFTVSIPLALDRAGRPDADIGAIVGLAA
                     LVQIPAALVGGVLIDRFGSMKLILVGGLSYIAATVFLILPGLAPETSTLEFIAARILQ
                     GLGFGMVMPAALSFVPRLVPVAKRGIALAMASTSHNLTLVFVPPMSVIVLDGAGLTGV
                     ALFAGVAVTAALVIAFARPFGARESGEDAEHHQARRLLGFAFRRAWVMPLAIIGLFDI
                     HWGVVTAYLPQRAEAADADIGLFFAADGLLVLLTRMPAGWLADRAPSSWQIIAGVVAT
                     FVGIALILPAPTTPILILSGALTGAGAALIIVPINLALTRRSNDADRGSAFALFSAIF
                     AAGIALGSIGTAPLIDVVGYEVLLGASLVALIAAIGVTLLDRDLGSVPVSAKAEESVA
                     ATPAGG"
     gene            1292..2464
                     /locus_tag="VMZ33_01270"
     CDS             1292..2464
                     /locus_tag="VMZ33_01270"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HUR15889.1"
                     /translation="MPKELMKIGVVKERAPNERRVALVPDALKPLLAAGAEILVERGA
                     GDGAAIPDKLFVDAGATIASHEDLYEQADVIVRVQRPEADDIAGLREGQTVIGLLSPL
                     IDPQLMQTLAGKKVTAISLDAIPRTLSRAQTMDVLSSQANVGGYRAVLMAAEAYGRYF
                     PLLTTAAGTARPANVLVLGVGVAGLQAIATARRLGAIVTAYDVRSETKEQAESLGARF
                     IVLKSVADASGTGGYARELTAEEREAQQNELNGHIAGMDVIITTAQVPGRRPPLLVTG
                     TAVVGMKSGSVIVDMAASALGGNVELSKADETVVTDNGVTILAPTNLPATMPAGASQF
                     YARNLAAFLTHLLKDGALTLDMGDEITAATVITRDGAVVQEQTKKLLEPKAAEGAK"
     gene            2461..>2775
                     /locus_tag="VMZ33_01275"
     CDS             2461..>2775
                     /locus_tag="VMZ33_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HUR15890.1"
                     /translation="MSQDLLNALAIFVLAILVGFEVISKVPATLHTPLMSGANSIHGI
                     VLVGAMLIAATADNPLSYLLAFIAVVFGAANVVGGYVVTDRMLQMFRKRETPAAKPAD
                     EAA"
BASE COUNT          429 a          934 c          911 g          501 t
ORIGIN      
        1 cgtgctgcgg ctctccgtaa cgatcttcct gattcaggcc ggattccacg cattcacggt
       61 ctcgatccca ctcgccctcg accgggccgg ccgccccgac gccgacatcg gcgcgatcgt
      121 gggtctggcc gcgttggtcc agataccggc ggcgctcgtg ggcggggttc tgatcgaccg
      181 cttcggcagc atgaagctca tccttgtcgg cggcctttca tacatcgccg caaccgtctt
      241 cctgattctc cccggactcg ctccggagac ctcgacgctc gagttcatcg ctgcccgcat
      301 cctccagggt ctcgggttcg ggatggtcat gcccgcggcc ctgtcgttcg tgccgcggct
      361 ggtccccgtg gccaagcgcg gaattgccct ggcgatggcg agcacgtcgc acaacctgac
      421 gctggtgttc gtgccgccga tgtcagtgat cgtcctcgac ggtgcgggac ttaccggcgt
      481 tgcgctattc gccggggttg cggtcacagc ggcactcgtg atcgcctttg cgcggccttt
      541 cggcgcacgc gagagcggcg aagacgcgga gcatcaccag gcgaggcgcc tgctgggctt
      601 tgccttccga cgcgcatggg tcatgcccct cgcgatcatc ggcctgttcg acatccactg
      661 gggcgtagtc actgcctacc ttccacagcg ggctgaggca gcggacgccg atatcgggct
      721 cttcttcgcc gccgacggac tactcgtcct tctgacacgc atgcccgcgg gttggttggc
      781 cgatcgcgcg ccgtcgtcct ggcagatcat cgctggagtc gttgccacat tcgtcggtat
      841 cgcgctcatc ctgccggcgc cgacgacccc gatcctgatc ctctcaggcg ccctgaccgg
      901 tgccggcgcg gcgctgatca tagttccgat caacctggcc ttgacccgcc gcagcaacga
      961 cgccgatcgc ggcagtgcct ttgccctgtt ctcggcgatc tttgccgcgg gcatcgcgct
     1021 tggctcgatc ggcacggcgc cactcatcga tgtcgtgggc tacgaggtcc tcctcggcgc
     1081 ttcgctggtg gctctcattg cggcgatcgg cgtcacgctg ctcgatcgcg atctggggtc
     1141 cgtgccggtc agcgccaagg ctgaggagtc ggtcgcggca acaccggctg gcggctgatc
     1201 gggcggttgc gcgggcgcct ttggggccgg cctcggtccg agaggtgaat cggccaaagg
     1261 ccgctatcct tcgcctactt tccccagcat tttgcccaag gaactcatga agatcggtgt
     1321 tgtaaaggag cgcgcgccca acgagcggcg tgtggcgctc gtaccggatg cgctgaagcc
     1381 gttgctcgcg gccggcgcgg agattctcgt cgaacgtggg gccggagacg gcgccgcgat
     1441 ccctgacaag ctgttcgtcg acgccggcgc gacgatcgcc tcgcacgaag acctctatga
     1501 gcaggccgac gtgatcgtgc gggtccagcg acctgaagcg gacgacatcg cgggtttgcg
     1561 cgagggacag acggtcatcg ggctgctgtc gcccctcatc gatccgcagc tgatgcagac
     1621 gctcgccggc aagaaggtca ccgcaatcag tctcgatgcc atcccgcgga cgttgtcgcg
     1681 cgcccagacc atggacgtgc tgtcgtcgca ggcgaacgtt ggtggctacc gcgccgtcct
     1741 catggccgcc gaggcctacg gccgctactt cccgctactc accaccgccg cgggtaccgc
     1801 caggccagcg aacgtgctgg tcctgggcgt cggcgtagcc gggctccagg cgatcgccac
     1861 tgcccgacgg ttgggcgcga tcgtcacggc gtacgacgtc cgatcagaga cgaaggagca
     1921 ggccgaatcg ctcggcgcca ggttcatcgt cctgaagtcg gttgcggacg cctccggcac
     1981 ggggggctac gcacgcgagc tgacggccga ggagcgtgag gcgcagcaga acgagctaaa
     2041 cgggcacatt gcgggcatgg acgtgatcat cactaccgcc caggtacccg gccgccggcc
     2101 gccactgctc gtcacgggga cggctgttgt ggggatgaag tccggctcgg tgatcgtcga
     2161 tatggcggcg agcgccctcg gcggaaacgt cgagctgtcg aaggccgatg aaaccgtcgt
     2221 gactgacaac ggcgtgacga tactggcgcc gactaacctg ccggccacaa tgcccgctgg
     2281 cgcaagccag ttctacgcgc gcaacctcgc agcgttcctc acccacctgt tgaaagacgg
     2341 tgccttgacg ctcgacatgg gggacgaaat caccgctgcg actgtcatca cgcgcgacgg
     2401 cgcggtcgtg caggaacaga caaagaagct gctcgaaccg aaggccgcgg agggagccaa
     2461 gtgagccagg atctactcaa tgcgctggcg atcttcgtcc tggcgatcct cgtcggattc
     2521 gaggtcatca gcaaggtgcc agcgacgctg catacgccgc tgatgtccgg cgccaactcg
     2581 atccacggca tcgtgctcgt gggcgcgatg ctcatcgctg ccacggctga taacccgctg
     2641 agctacctcc tggcgttcat cgccgttgtg ttcggcgccg ccaatgtggt cggcggctat
     2701 gtcgtgacag accggatgct ccaaatgttc cgcaagcggg agacccccgc ggccaagccg
     2761 gccgatgagg cagcg
//
LOCUS       DATLCO010000048         3405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2290007, whole genome shotgun sequence.
ACCESSION   DATLCO010000048 DATLCO010000000
VERSION     DATLCO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2290007"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VMZ33_01280"
     CDS             complement(<1..351)
                     /locus_tag="VMZ33_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR15891.1"
                     /translation="MTARTRLSAQPDAHAGYVHRDTWNPPQIMGRRVALRRPTPSDLP
                     HLKHWYRDGEIARLTRYQERPMTEQEVERFFHSRMLTPDALAYCIVELPDWRLIGFTT
                     FSALDVDNGSVMFHI"
     gene            390..2111
                     /locus_tag="VMZ33_01285"
     CDS             390..2111
                     /locus_tag="VMZ33_01285"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HUR15892.1"
                     /translation="MRRWSEVANRIAATTRTTEKVGTLAEYLRSLSAAELPIAVTYLT
                     GRPFPASDPRSTGIGWAAIAKVAEAIVDAGPGALGAAYNQSSDLGQAVGDLFAEHGHE
                     GDTQQPTLPEVQAAYSKVAVARGQAKHAPLRDLLNRCDPLTAKYAVKVLSGDMRIGLR
                     EGHLEAGIASAFERPLADVQWAGMLTGDLGQTAELARDDRLGDAELALFRPLKSMLAA
                     PVVDEHEVIRRFGAPVWTEDKYDGIRAQLHKQGSTVKLYSRDLHDVSDGYPEVIAVAR
                     TLPWDGILDGELLAYRDGMAMPFIQLQARLGRKNPSVEMQAQVPVIYVAFDALAVGPG
                     SGAAIEPLLRRTLRERRERLDTLGLPLTPGMATANLLTAATAEELQGIFDEAQDRGNE
                     GLMVKDPDSGYTPGRRGYGWLKLKRPLATLDCVVVGVEVGHGKRHGVLSDYTFAVGDD
                     RPEAPEGRLVTIGKAYSGLTDAEIAEMTLWFEEHTIRRIGRFHVVEPSIVVEIAFDVI
                     HRSNRHDSGYALRFPRIFRLRPDKDASEIDTLSNVDVLYRRLESGRRFSVHAEAGAAP
                     PTTITVD"
     gene            2117..3055
                     /locus_tag="VMZ33_01290"
     CDS             2117..3055
                     /locus_tag="VMZ33_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15893.1"
                     /translation="METDEDKVARLREQIPATSAGIYLDTAYRGPLPAETATAMREAD
                     DWELRVGRATDGRDHDVLQRHDEARAVLAALIGADLDAITVTPGIESAAGLVQNVLGV
                     GPERIQGHVDPSTGEVLADPASEGSVVLDVSMSAGAIPLMVANLNADALIFATDRWLL
                     GPENLACVWLKQPVAGIEMAIARTPLLGLARTVGWLEMYVGLEWIYARGTRLAGLLHD
                     LLSATAGVDVVTPREAMATIVSFRLPAWPVDDGLAEIRRRCFAIIGSSPHGDLIRASI
                     AWFNTDEEIERFANAVAEIARHTPETLPRRPTLVVH"
     gene            3067..>3405
                     /locus_tag="VMZ33_01295"
     CDS             3067..>3405
                     /locus_tag="VMZ33_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15894.1"
                     /translation="MPSQRSWLEIQWRKLRNPPPPVFRAVVGNLLVAGVAGVLLLIYD
                     LLNPTADLAPLVVAYVAIVVVAGSVLTYLWVELPTGASGVRRRSGWAGLLGFFAAIPI
                     AYLTLVVLFQV"
BASE COUNT          550 a         1140 c         1145 g          570 t
ORIGIN      
        1 gatgtggaac atgaccgatc cgttatcgac gtcgagcgcc gagaacgtcg tgaagccgat
       61 caagcgccaa tcaggtagct cgacgatgca ataggcaagc gcgtctggcg tcagcatgcg
      121 gctgtggaag aagcgctcga cctcctgctc ggtcatcggt cgctcctggt agcgcgtcag
      181 acgggcaatc tcgccgtcgc gataccagtg cttgaggtgc ggcagatcgg acggcgtcgg
      241 acgcctcagc gcaaccctcc gccccatgat ctggggtggg ttccacgtgt ctcgatgaac
      301 gtagccggca tgcgcgtccg gctgcgcgct cagtctggta cgggcggtca cggccgcgat
      361 catacgaccg gcactaacat ccgagccgga tgcgtcgctg gagcgaagtc gccaaccgta
      421 tcgcggcgac gacacgaacc actgagaaag tcggcactct cgctgagtac ctgcgctcgt
      481 tgagcgcggc cgagctgccg atagccgtca cctacctgac cggccgcccg ttccctgcga
      541 gcgacccgcg ctccaccggg atcggctggg cggcgatcgc caaggttgcc gaagcgatcg
      601 tcgacgccgg accgggcgcc cttggtgccg cctataacca atcatctgac ctgggccagg
      661 cagtcggcga cctgttcgcc gagcacggcc atgagggtga cacgcaacag ccgacgctcc
      721 ccgaggtcca agccgcgtac tcgaaggtcg ctgtggcccg tgggcaggcc aaacacgcgc
      781 cgctacgcga ccttctcaat cggtgcgatc cacttacggc gaagtacgcc gtcaaggtgc
      841 tcagcggcga catgcgcatc ggcctgcgcg agggccatct cgaggccggc attgcctcag
      901 cttttgaacg gccgctggcc gacgtccagt gggcgggcat gctcacgggc gaccttgggc
      961 agacggccga gctggcccgc gacgaccggt tgggtgacgc cgagctggcg ctgttccgac
     1021 cgctcaagtc gatgctcgcc gcgcctgtgg tggacgagca cgaggtcatc aggcgtttcg
     1081 gcgcgccggt ctggactgag gacaagtacg acgggatccg cgcccagctg cacaagcaag
     1141 gctcgacggt gaagctctac agcagggacc tccacgacgt aagcgacggc tacccggagg
     1201 tgatcgcggt cgcccgcacg ctgccgtggg atggcatcct cgacggcgag ctgctcgcct
     1261 atcgagacgg catggctatg cccttcatcc agctccaggc acgcctcggt cggaagaatc
     1321 cttcggtgga gatgcaggcg caggtgccgg tcatctacgt cgccttcgac gccctcgcgg
     1381 tcggtcccgg gagcggcgca gccatcgagc cgctgctccg ccggacgttg cgcgagcgtc
     1441 gcgagcgact cgacacgctg ggcttgcccc ttacgccggg gatggccacg gccaaccttc
     1501 tgaccgcagc aacggcggag gagctgcagg gcatcttcga cgaggcccag gaccgcggca
     1561 acgagggcct gatggtcaag gatcccgaca gcggctacac ccccggtcgg cgcggctacg
     1621 gctggctcaa gctcaagcgg ccgctggcga cgctggattg cgtcgtggtc ggggtggagg
     1681 tcggccatgg caagcgccat ggcgtgctgt ccgattacac gtttgccgtc ggcgacgacc
     1741 ggccggaggc gcctgagggc cgattggtga ccatcggcaa ggcctacagc gggctaacgg
     1801 acgccgagat tgccgagatg acgctgtggt tcgaagagca cacgattcgc cgcattggcc
     1861 gcttccacgt ggtcgagccg agcatcgtgg tcgagatcgc gttcgacgtg atccatcgct
     1921 cgaaccgcca cgactcgggg tacgcgctcc ggttcccacg catcttccgg ctgcggcccg
     1981 acaaggacgc cagcgagatc gacacgctga gcaacgtcga cgttctatac cggcgcttgg
     2041 agtccggccg tcgtttcagc gtccacgcgg aggccggcgc tgcgccacca accacgatca
     2101 cggtcgactg actgcgatgg agacggacga ggacaaggtc gcccgactgc gtgagcagat
     2161 tccagctacg tcggcgggta tctacctcga cactgcgtac cgcgggccac tccccgcgga
     2221 gacggccact gccatgcgcg aggcagacga ctgggagctg cgtgtcggcc gagcgactga
     2281 tgggcgcgat cacgacgtcc tgcagcgtca cgacgaggca cgcgctgtgc tggcggcgct
     2341 gatcggcgcc gaccttgacg cgatcacggt cacacctggg atcgagtctg ctgcgggact
     2401 ggttcagaac gtgctgggag ttgggccgga gcggatccag ggccacgtcg acccctcgac
     2461 tggggaggta ctggcagatc cggcgtcgga aggttcggtg gtgctggacg tgtcgatgtc
     2521 tgccggcgcc atcccactga tggttgccaa cctgaacgcc gacgcgctga tcttcgccac
     2581 ggaccgctgg ttgctaggcc cagaaaacct cgcctgcgtt tggctcaagc agccggtcgc
     2641 aggcatcgag atggccatcg cccgcacgcc gctgttgggg ctggcccgca cagtgggatg
     2701 gctggagatg tacgtcggac tggagtggat ctacgcgcgc ggcacccgcc tcgccgggct
     2761 gctccacgac ctgttgtcgg cgacagctgg agtcgatgtc gtcacgcccc gagaggccat
     2821 ggcgacgatc gtttcttttc ggctgccagc gtggccagtt gatgacgggc tcgccgagat
     2881 tcgccgacgt tgcttcgcga tcatcggctc ttcgccgcat ggggacctga tccgggcgtc
     2941 gatcgcgtgg ttcaacactg acgaggagat cgaacgcttc gcgaatgccg tcgcggagat
     3001 cgcgcggcac acacctgaga cactgccgcg ccgtcccacc ctggtcgtcc attgaccgag
     3061 ccaggcgtgc catcgcagcg ctcctggctg gaaatccagt ggcgcaagct gcgcaacccc
     3121 ccgccgccgg ttttccgggc ggttgtcgga aacctcctcg tggccggcgt cgcaggcgtg
     3181 ctgctgctca tctacgacct cctcaacccg accgccgacc tcgcgcccct ggtcgtcgcc
     3241 tacgtcgcca tcgtggtcgt cgccggcagc gtcctcacct atctttgggt cgagctgccc
     3301 accggcgcgt cgggcgtccg tcgtcgttcc gggtgggcgg ggctgctcgg cttcttcgcc
     3361 gccatcccga tcgcgtacct gacgctcgtc gtcctcttcc aggtg
//
LOCUS       DATLCO010000049         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2290463, whole genome shotgun sequence.
ACCESSION   DATLCO010000049 DATLCO010000000
VERSION     DATLCO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2290463"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VMZ33_01300"
     CDS             <1..358
                     /locus_tag="VMZ33_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR15895.1"
                     /translation="GDGPLYLGEPVGFVIELEDGKRVYYTGDTDVFGDMDLVRRLHKP
                     DVAIMPIGGHYTMGPAGAALAAKLLDVSAVIPVHYGTFPILAGSPDELRAELRSVGAG
                     NVEVVAPDRGVETPLP"
     gene            367..936
                     /locus_tag="VMZ33_01305"
     CDS             367..936
                     /locus_tag="VMZ33_01305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR15896.1"
                     /translation="MRVADPERDAARVVAIYRPHVVDGLASFEEVVPTVAQMAARIRT
                     TLTWTPWLVAVEPESDLVIGYAYASRHRERVGYRWSVDISVYVDEAWHRRGVGGQLYA
                     ELLALLRRQGFINVYAGITTPNPGSVRLHEQLGMKPIGTYERVGYKHGQWLPVTWFGM
                     RLQEPTIPPAEPIAMSELATDGQPAIKGE"
     gene            complement(923..3178)
                     /locus_tag="VMZ33_01310"
     CDS             complement(923..3178)
                     /locus_tag="VMZ33_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HUR15897.1"
                     /translation="MIDQLTHAWRAAVDRDRAQVERAREVEDPADFYAPVTSRFRLDP
                     RRTDDDTLNALLALALREDVWVDVGAGGGRYALPLALQTHEVIAIDSSPSMLRALRDD
                     AAANGIENVRVIEARWPMTDPPAGDVGLMAHVGYDIAEIGAFLDQLEAQSRRLCVAVL
                     GESAMTTVATLFWQEIHGEPRVRLPALSELVALLRARGRLPSIQLVDRTPPSFSSFDE
                     ALAMARRQLWLRQGSPNDQRLRELAQGTLVEREGRYAFEWQPTKIGIVTWVPPGEGPR
                     VEDDMTATQLTQQLDPVLARLAAAIDRYDAEIDSSRVAPNVAPAEIRAHLSAYTFEDA
                     IPLPDLVDQVASMLHQWSLHVTHPRYFGLFNPTVRRAGVFADALAALYNPQLAAWPHA
                     PIANEIERHVLARFMRAFGLDPETGIANFTTGGQEANQTAVTVALTRAFEAYGERGVG
                     ALDGPPVLYVSGEAHHSFVKIAHQSGIGRASVRHVRVDAQLRMDVDDLRRQLDEDKAR
                     GAIPFLIVATAGTTAAGAIDPIPELAAIAAEHRLWLHVDAAWGGAAIVAPSLRHHLAG
                     IELADSITCDAHKWMSVPVGAGMFFCRHREPTLQAFRVEASSYVPAAVPDTFDPYVSS
                     IQWSRRFIGLKLFMVLAELGWDGLGELVAEQARTADLLRSLLAERGWRIVTDSPLAVV
                     CFTHPDLESGAVTVGAVVDRLVAGGRAWISHVRLAESAVALRACITSHRTTRQDLDVL
                     LDELDRAITPP"
BASE COUNT          578 a         1112 c         1150 g          530 t
ORIGIN      
        1 cggcgacgga cccctgtacc tgggcgagcc cgtcgggttc gtgatcgagc tggaggacgg
       61 gaagcgcgtc tactacacgg gcgacacgga cgtgttcggg gacatggacc tcgtccgacg
      121 cctccataag cccgacgttg cgatcatgcc gatcggcggt cactacacga tgggtccggc
      181 aggcgcggcg ctcgccgcga agctactgga cgtgagcgcc gtgatcccgg ttcactacgg
      241 cactttcccg atcctggccg gcagccccga cgaattgcgg gcagagctcc gctcggtcgg
      301 cgccggaaac gtcgaggtcg tcgcgcccga ccgcggcgtc gagactccac tcccctgacg
      361 gcgctcatac gcgtcgccga tccggagcgc gatgcggcgc gcgtcgtcgc gatctaccgc
      421 ccgcacgtcg tcgacggact cgcgtccttc gaggaggtgg tgccgaccgt cgcccagatg
      481 gccgcacgca tccggaccac actgacctgg acgccgtggc tagtggcggt cgagcctgag
      541 tccgaccttg tgatcggcta cgcctacgcg tctcggcacc gcgagcgcgt cggttaccgg
      601 tggtcggtcg atatttcggt ctacgtcgac gaggcgtggc accgtcgcgg tgtcggcggg
      661 cagctgtacg ccgagctttt ggcgctccta cggcgacagg gcttcatcaa cgtctacgcc
      721 ggcatcacga cgcccaaccc cggaagcgtc cgccttcatg agcagctggg aatgaagccg
      781 atcggcactt acgagcgcgt cggctacaag cacggtcagt ggctgccggt cacgtggttc
      841 ggcatgcgac tccaggagcc gaccatcccg ccggcagagc ccatcgccat gtctgagctg
      901 gccaccgacg gccagccagc catcaagggg gagtgattgc gcgatcgagc tcgtccagga
      961 ggacgtcgag atcctggcgt gtcgtacggt gtgacgtgat gcaggcgcgg agcgccactg
     1021 ccgactcagc gagacgtaca tgagagatcc aggcccttcc gcccgctact agccggtcca
     1081 cgaccgcgcc caccgtgacc gccccactct ccaggtccgg gtgcgtaaag cagacaaccg
     1141 ccagtggcga gtcggtgacg atgcgccaac cgcgctctgc gaggagcgag cgcagcagat
     1201 cggcggtgcg cgcctgctcg gcgactaatt cgcccaggcc gtcccagccg agctcggcca
     1261 gaaccatgaa cagcttcagg ccgatgaacc tgcgcgacca ttggatcgaa ctgacatacg
     1321 gatcgaaggt gtcgggcacg gcagccggga cgtacgacga cgcctcaaca cgaaaggcct
     1381 gcagcgttgg ctcgcggtga cggcagaaga acattccggc cccgaccggg acggacatcc
     1441 atttgtgggc gtcgcatgtg atcgagtccg cgagctcgat cccggccaga tgatgacgca
     1501 gggagggcgc cacgatcgcg gcgccacccc aggcggcgtc cacgtgcagc cacagccggt
     1561 gctcagcggc gatcgctgcc agctcgggta tcggatctat agccccggcc gccgtcgtgc
     1621 cggccgtcgc cacgatcagg aacgggatcg cgccgcgcgc cttgtcttcg tcaagctggc
     1681 gccgcagatc gtcgacgtcc atgcgcagct gcgcgtcaac gcgaacgtgt cgaactgacg
     1741 ctcggccaat tcctgattgg tgagcaatct tgacgaacga gtggtgggcc tctccggaga
     1801 cgtaaagcac gggcgggccg tccagagcgc cgacgccgcg ttcgccgtat gcctcgaacg
     1861 ccctcgtcaa cgctacggtc acggctgtct ggttggcctc ctggccaccg gtcgtgaagt
     1921 tggctatgcc cgtctcgggg tcgagcccga aggcgcgcat gaatcgcgcc agaacgtggc
     1981 gctcaatctc gttggcgata ggcgcgtgtg gccaagccgc cagctgtggg ttgtagaggg
     2041 cagccaaggc gtccgcgaag acgcccgccc gccgaaccgt tgggttgaag agcccgaagt
     2101 aacgcgggtg ggtaacgtgc agcgaccatt gatggagcat cgaggcgacc tggtcgacca
     2161 ggtcggggag cgggatggca tcctcaaagg tgtaggcact gaggtgggcc cggatctctg
     2221 ccggggcgac gttcggggcg acccgagaac tgtcgatctc ggcgtcgtat cggtcgattg
     2281 ccgcggcgag ccgcgcgagg acaggatcca gttgttgagt gagctgagtt gctgtcatat
     2341 cgtcctcaac gcggggccct tcaccaggcg gcacccacgt gacgataccg atcttcgtgg
     2401 gctgccactc gaaggcgtag cggccctccc gctcgacaag tgtgccctgc gccagctcgc
     2461 gaagccgttg atcgttcggc gagccctggc gcagccagag ctggcggcga gccatcgcca
     2521 gggcttcgtc gaaagatgag aacgacggag gcgtccgatc gaccagctgg attgacggca
     2581 gtcgaccacg cgcacgcagc agcgccacga gctcggacag cgcgggcagt cggacccgtg
     2641 gctcgccgtg gatctcctgc cagaacaggg tggcgactgt ggtcatggcc gactccccaa
     2701 ggaccgccac acacaggcgt cgcgactgcg cttccagctg gtcgagaaac gcgccgatct
     2761 cggcgatgtc gtagccgaca tgcgccatca gcccgacgtc gccggctggc ggatccgtca
     2821 tgggccagcg cgcctctatg acgcgcacgt tttctatgcc attcgccgcg gcgtcgtcgc
     2881 gaagtgcgcg gagcatcgac ggtgatgagt cgatcgcgat cacctcgtgc gtctgcaggg
     2941 cgagcggcag cgcgtaacgg ccgccgccgg caccgacgtc gacccacacg tcctcccgca
     3001 aagcgagcgc cagcagcgca ttgagcgtgt cgtcgtccgt tcgtcgcggg tccaggcgga
     3061 agcgcgaggt caccggggcg tagaaatcgg ccgggtcttc gacctcccgt gcccgctcaa
     3121 cctgcgctcg gtcgcggtcg acggcggcgc gccaggcgtg ggtcagctgg tcaatcaccg
     3181 gccgagcgta gccgctcgac ctacactcga aactgagatg ggtgacgaaa tggagttggg
     3241 cgaggatcgc gcgcggcgta tcgcgctgct caatgatctg atcaagacgc gcatcctggt
     3301 catggacggc gcaatgggca cgctcgtgca gcgctatgag ctgaccgagg aggagttccg
     3361 cggcgagcgc
//
LOCUS       DATLCO010000050         5344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2295477, whole genome shotgun sequence.
ACCESSION   DATLCO010000050 DATLCO010000000
VERSION     DATLCO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2295477"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VMZ33_01315"
     CDS             complement(<1..688)
                     /locus_tag="VMZ33_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUR15898.1"
                     /translation="MSLTTTTRIAGLSVAAALVLTACAGNSATTAPSSEGSFAPSAAA
                     SGEIPTGVSGNVDIHGSSTVAPISNAIAEDLAAANPDFSYFVGDEGTGAGFTEFFCTG
                     DGDISDASRKIRAENGDTPDPEESSVCADNGVEYVELPVAYDGLAVITALGNPLECLS
                     FADLYALLGPEADDVANWNDAEALAHELGSTTDLPDAPLAVTAPGDESGTYDSFIELA
                     LGDLIEERAAE"
     gene            complement(766..2271)
                     /locus_tag="VMZ33_01320"
     CDS             complement(766..2271)
                     /locus_tag="VMZ33_01320"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HUR15899.1"
                     /translation="MPLPGPARADEELELKYTIDDPGAAEGWFDEHYPDREGWRTVRM
                     VDRYFDTAGRALFDAGYGARLRQVGRKTVLTLKSDIDVTDGRHQRLELEGHATQELDV
                     NAWKPSAARDRVAEVVGERRLIELFLVRQKRRERPISVDGAELMVSIDLGKVVAAGRE
                     VGPIVQLEVEHLRGRRVPLDEVARQIREAGIGRPESRSKLAMAHELAEAASVVAADDK
                     LAEAGRKVLRRHLVRLLSREPGARDGDELETKQMRVATRRLRATWGVFADAFKARVAR
                     ERQHALRDIGRAIGEVRDLDVLIATVENKPALTPLTDSWRAQRAAAHDDLVRLLDSKR
                     YRRFVDEMLEFTASPGKGARRKRGSRPVAEAISTALQTAVESLHVTESLTPQTADVDA
                     AWHARRIEARRLRYSVEAFLDVLDETPSRKLLDSITRLQDRLGALQDGTVAMEKVGGW
                     LDEQDGALPEELQSAVDAYLDRRLRDVEKSRIGLDAAWSALMTQAEALTKR"
     gene            complement(2293..4476)
                     /locus_tag="VMZ33_01325"
     CDS             complement(2293..4476)
                     /locus_tag="VMZ33_01325"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HUR15900.1"
                     /translation="MAALRTPAEIETGDTSQQTPDRPQPQRSNGRARRMLPADMPYIN
                     RELSWLEFNDRVLFEAVDARNPLLDRVRFLSIFAGNLDEFFQVRVAGLKQQVAAGRSS
                     PTPDGMGAVQTLDAVRTRLLPMVARHSDTFSQIRQELAEEGVRIVRYDERPERHVELR
                     RRFFDEIFPVLTPLAVDPGHPFPYISDLSLSLAVTVRDPQTGERLFARIKVPPILPRL
                     MEVGQRTYVLIDQVIAANLDALFPGMEVVEYHLFRVTRNADLSIEEEEAPDLLEAIEE
                     ELRKRRFGKVVRLEIERSMPHTTRTLLMRGLDIAPEDVYEVSGMLDLTGLTEIAELDV
                     PHLHDEPWLPVTPPRLAPPDPDEEADVFAALRAGDVLLHHPYESFTTSVQRFIEQAAE
                     DPDVLTIKQTLYRTSGDSPIVAALIEAAEAGKQVVVLVEIKARFDERANIVWARKLER
                     AGAHVAYGLVGLKTHSKIALVVRREGRGLRRYVHIGTGNYNNKTARVYSDLGLLTADQ
                     ELGADVTDLFNRLTGHSRQKRYRKLLVAPTTLRDGLVDLINRERERQRDHGDGRIVIK
                     CNSVVDPAVIAALYRASAAGVPIDIIARGMCAVRPGVPGYSETIRVRSIVGPFLEHSR
                     IYVFGAGDRERFWIGSADIMERNLDRRVEALAPVEDSDSRAKLRQIIDIMLGDDRRAW
                     QLAADDRWRRVEELAEKPGGTDTFEELMAAARQQLVDVNAKPDDA"
     gene            complement(4973..>5344)
                     /locus_tag="VMZ33_01330"
     CDS             complement(4973..>5344)
                     /locus_tag="VMZ33_01330"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HUR15901.1"
                     /translation="PSRVHADRAASTLELEWPDGHATIYDFETLRWLCPCAYCRGEAG
                     MPGWLDSNPTLSPAQTQLTDIALVGNYAIQPTWADGHHTGFYTFLRLREDCPCAEDAE
                     RRARERDNTGDSAQSAARRLG"
BASE COUNT          818 a         1755 c         1832 g          939 t
ORIGIN      
        1 cctcagcggc gcgctcctcg atcaggtcgc cgagcgccag ctcgatgaag ctgtcgtacg
       61 tgcccgactc gtcgcccggg gccgtgaccg cgagtggcgc gtcaggcagg tccgtggtcg
      121 agccgagctc gtgtgcgagt gcctctgcgt cgttccaatt cgcgacgtca tcggcctcag
      181 gtccaagcag cgcatacagg tccgcgaatg agaggcattc aagcgggttg ccgagcgcgg
      241 tgatgacggc caggccgtcg taggcaaccg gcagctcgac gtactcgacg ccgttgtcgg
      301 cgcaaacgct cgactcctcg ggatcaggcg tgtcaccgtt ctcggcgcgg atcttgcgcg
      361 aagcatcgga gatgtcgccg tcaccggtgc agaagaactc ggtaaagccg gcgcccgtgc
      421 cttcgtcgcc gacgaagtag ctgaagtcag gattggctgc ggcgagatcc tcggcaatgg
      481 cattggagat cggagcgacg gtcgatgagc cgtggatgtc gacgttgcca ctgacgcctg
      541 tcgggatttc gccgcttgct gccgcgcttg gagcgaagct gccttcggag ctcggggcgg
      601 ttgtcgccga attgcccgcg caggcagtca ggacaagtgc ggccgcgacg ctgagaccgg
      661 cgattcgcgt ggtggtcgtg agggacacga gcgatttctc tcctctactg gacgctttac
      721 cggtccagcg tcgggttcgc tcatcaacgc cgcattggcg gttcgttaac gtttcgttaa
      781 cgcctcggcc tgtgtcatga gggccgacca cgcggcatcc agccctatcc gcgacttttc
      841 gacgtcgcgc agccggcgat ccaggtacgc gtctacggcc gactgcaact cctcaggcaa
      901 agctccgtct tgctcgtcga gccagccacc gaccttctcc attgcgacgg tgccgtcctg
      961 taaggcgccc agccgatcct gcagcctggt gatcgagtcg agaagcttgc ggctgggggt
     1021 ttcgtccagc acgtccagga acgcctcgac ggagtagcgg agtcggcgcg cctcgatacg
     1081 acgcgcgtgc cacgcggcgt cgacgtccgc ggtttgcggc gtgagcgatt ccgtcacatg
     1141 caggctctcg acggcggttt ggagggcagt ggaaatcgcc tcggcgacgg gccgcgagcc
     1201 acgctttcgg cgcgcgccct tgcccggtga cgccgtgaac tcgagcatct cgtcgacgaa
     1261 gcgccggtag cgcttcgagt cgaggaggcg aaccaggtcg tcgtgagccg cggctcgttg
     1321 agcgcgccat gaatcggtga gcggcgtgag agccggtttg ttctcgaccg tcgcgatcag
     1381 cacgtccagg tcgcggacct cgccgatggc acggccgatg tcgcgcagcg cgtgttggcg
     1441 ctccctcgcg acgcgcgcct tgaacgcgtc agcgaacacg ccccacgtcg cacgcagccg
     1501 gcgcgtggcg acacgcatct gcttggtttc gagctcatcg ccgtcgcgtg ccccgggctc
     1561 gcgcgagagc aggcgcacga ggtgtcgacg cagcacttta cggccggcct cggccagttt
     1621 gtcatccgct gcaacgacgc tcgcggcctc ggccagctcg tgtgccatgg cgagcttgct
     1681 gcgcgactcc gggcggccga tgcccgcttc tctgatctgc cgcgcgacct catcaagtgg
     1741 gacgcgccgg ccgcgcaggt gttcgacctc gagttggaca atgggtccga cctcgcgacc
     1801 ggcggcgacc accttgccaa ggtcgatcga caccatcagc tcggcgccat cgacggagat
     1861 gggtcgctct cggcgctttt gacgaacgag gaacagctcg atcagccgtc gctcgccgac
     1921 cacctcagcc acccggtcac gcgccgcgct cggcttccac gcgttcacgt cgagctcttg
     1981 ggtagcgtgg ccctcaagct ccagacgttg gtgtcggccg tccgtcacat cgatgtcgga
     2041 cttcagggtc aaaacggtct tgcggcctac ctggcgtagg cgcgcgccgt agccggcatc
     2101 gaaaagagcc cgtccggccg tgtcgaagta gcggtcgacc atccggaccg tccgccatcc
     2161 ctctcggtcg ggatagtgct cgtcgaacca gccctcggcc gcgcccggat cgtcgatggt
     2221 gtatttgagc tccagctcct cgtcagcccg cgcgggaccg gggaggggca ccacgtcggc
     2281 catcagcgcg gcttaagcgt cgtcaggctt ggcgtttacg tccaccagct gctgacgcgc
     2341 agcggccatc agctcctcga acgtgtcggt gccgcccggc ttctcggcca gctcctcgac
     2401 gcgccgccaa cggtcgtcgg cggcgagctg ccacgcgcgc cggtcgtcgc cgagcatgat
     2461 gtcgatgatc tggcgcagct tcgcgcggct gtcgctgtcc tccaccggcg cgagtgcctc
     2521 tacgcggcgg tcgaggttgc gctccatgat gtcagccgat ccgatccaga agcgctctcg
     2581 gtcgccggcg ccgaaaacgt agatacgcga gtgctcgagg aacggaccca cgatcgagcg
     2641 gacgcggatc gtctcgctgt agcccggcac gcctggccgg accgcgcaca tgccgcgggc
     2701 gatgatgtcg atcggcacgc cggcggcact ggcgcgatag agcgcggcta tgacggcggg
     2761 atcgaccacg ctgttgcact tgatcacgat gcggccgtcg ccatggtcgc gttgtcgctc
     2821 gcgctcgcga ttgatgaggt cgaccagccc atcgcgcagg gtcgtcggtg cgacgagcag
     2881 cttgcggtaa cgcttctggc gtgagtgacc ggtcagacgg ttgaagaggt cggtcacgtc
     2941 ggcgccgagt tcctgatcgg ccgtgagcag acccaggtcg ctgtagacac gggccgtctt
     3001 gttgttgtag ttgccggtgc cgatgtgcac gtagcggcgc aaaccgcgac cttcgcgtcg
     3061 aaccaccagg gcaatcttgg agtgcgtctt cagcccgacg agcccgtacg ccacgtgggc
     3121 gccggcgcgc tccagcttgc gtgcccacac gatgtttgcc cgctcgtcga agcgtgcctt
     3181 gatctcgacc aggaccacga cctgcttgcc cgcctcggct gcctcgatga gtgccgcgac
     3241 tatcggcgaa tcgccgctcg ttcgatagag cgtctgcttg atcgtgagca cgtcgggatc
     3301 ctcagcggcc tgctcgatga agcgctggac gctggtcgtg aacgactcgt acgggtggtg
     3361 cagcagcacg tcgccggcgc ggagcgcggc gaagacgtcg gcctcctcgt cgggatcggg
     3421 cggcgcgagg cgcggcggcg ttaccggcag ccacggctcg tcgtgcagat gcgggacatc
     3481 gagctcggct atctcagtca gccccgtaag gtcgagcatg cccgatacct cgtagacatc
     3541 ctcgggcgcg atgtccaggc cgcgcatcag cagtgtgcgc gtggtgtgag gcatcgaccg
     3601 ctcgatctcg agccgcacca ccttgccgaa gcgccgcttg cggagctcct cctcgatggc
     3661 ttcgagaagg tcgggtgcct cttcctcttc gatcgacagg tcggcgttgc gcgtgacgcg
     3721 gaacaggtgg tactccacga cctccatccc ggggaacagc gcatcgaggt tcgccgctat
     3781 gacctggtcg atcagcacgt acgtccgctg gccgacctcc atcaggcgcg gcaggatcgg
     3841 cgggactttg atgcgcgcga agaggcgctc accggtctgc gggtcacgga cggtcacggc
     3901 gagtgacagc gagaggtcgc tgatgtaagg gaacggatgg cccgggtcaa cggcgagcgg
     3961 cgtgagcacc gggaagatct cgtcgaagaa gcggcggcgc agctcgacgt ggcgctcagg
     4021 tcgctcgtcg tagcggacga tgcggacccc ttcctcggcc agctcctggc ggatctgcga
     4081 gaacgtgtcg gagtgccggg cgaccatcgg cagcagtcgc gttcgcaccg cgtcgagcgt
     4141 ctgtaccgcg cccatgccgt ccggggtggg gctcgaccta ccggctgcga cctgctgctt
     4201 cagaccggcg acgcgcacct ggaagaactc gtcgaggttg ccggcgaaga tcgacaggaa
     4261 acggacccga tcgagcagcg ggttgcgcgc gtccactgcc tcgaacagca cgcggtcgtt
     4321 gaactccagc cacgagagct cgcggttgat atagggcatg tccgcaggca acatgcgacg
     4381 ggcacggcca ttcgatcgtt gcggctgtgg gcggtccgga gtctgctggc tggtgtcgcc
     4441 agtctcgatc tcggccggcg tgcgtagtgc ggccatggac cggtcagtct agcaatcggc
     4501 ggttcacgct caggatggtg gtgcctgagc gcaccgcgcc gcaatcggcc gaacgtactt
     4561 atccgcgccg cctcgcacct ctgctcgctc ggcgaacgca ccccgtcgcg gtcgacggcc
     4621 ctggcgttct ctcagaaggt gcgtcggcta tgcgtgtccc gttgccacgc gtccgaagcc
     4681 gctcccatat gcctcacatt attggcggtg cggcctggca gcggttaggc cggtggaagc
     4741 gcgagatcga ccgtccaggt acgtcgctta acggcgaagt agccgctggt ttagacatgg
     4801 gctgcatgcc gaaccggata gcggccgtcc gctccggtca gggatggcgc aatcgtgctg
     4861 tcaccagcac ccgtaagcat gtggcgcata agggtgggtc ggggcgcgtc gtgcggcagc
     4921 cgaggcgggt cgtgcggcgg ccgaggctcg agcgctcatg cggaccccgc ggtcagccta
     4981 ggcgccgcgc ggccgattgg gcagagtcgc cggtattgtc acgctctcgt gcgcgccgtt
     5041 ccgcgtcctc agcgcagggg cagtcctcac gcagccgcag gaacgtatag aagcctgtgt
     5101 ggtggccgtc ggcccacgtc ggctgaatgg cgtagttgcc caccagcgcg atatcggtca
     5161 gctgtgtctg cgctgggctg agtgtcgggt tcgagtccag ccaaccgggc attcccgcct
     5221 ctccccggca gtaagcgcag gggcacagcc agcggagcgt ctcgaagtcg tagatcgtgg
     5281 catgcccgtc gggccactcg agctcgagag tcgacgcagc acggtcagcg tggacgcgac
     5341 tcgg
//
LOCUS       DATLCO010000051         3297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8999594, whole genome shotgun sequence.
ACCESSION   DATLCO010000051 DATLCO010000000
VERSION     DATLCO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8999594"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1038)
                     /gene="sucD"
                     /locus_tag="VMZ33_01335"
     CDS             complement(151..1038)
                     /gene="sucD"
                     /locus_tag="VMZ33_01335"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HUR15902.1"
                     /translation="MSILVNSETRLVVQGITGREGAFHALQMQEYGTNIVAGVTPGKG
                     GQFALEKPVPVFDTVAEAVAEAGANTSVIYVPAPGAPDAILEAVGAGIKTIFCITEHI
                     PALDMLRVVPVVDEAGARLVGPNCPGATSVGQAKVGIIPGSIHRPGRVGVVSRSGTLT
                     YEVVEALTEAGIGQSTCVGIGGDPINGSNFVDILKLFRDDPDTDAVVLIGEIGGDAEE
                     RAADWIRDNMTATPMAAFIAGRTAPPGRRMGHAGAIISGNRGTAESKVAALEAAGVRV
                     AQAPSEIPKLLQEAGVRPS"
     gene            complement(1035..2222)
                     /gene="sucC"
                     /locus_tag="VMZ33_01340"
     CDS             complement(1035..2222)
                     /gene="sucC"
                     /locus_tag="VMZ33_01340"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HUR15903.1"
                     /translation="MKLQEYHAKRLLSAAGLPVPEYAVASTAAEAREQAEGYLAAGCG
                     RVVIKAQVLVGGRGKAGGVKLASSADEAERVASQVLGMDIKGTTVRRVLVAPAADIVK
                     EYYLAVVLDRAARRALFMASAEGGVEIEVVAEERPDAITYSHAHAHLGLLDYQARGLA
                     FQLGLGAHLKEFVAIAKGLHTVMVEDDADLVEINPLAIVRAADGTETLQCLDAKITID
                     DSALHRHPDIEAMRDLDEEDPVDIAAREQGLSFIRLEGTIGCMVNGAGLAMTTMDLVK
                     LAGGQPANFLDIGGGARADKVAAAMRLILSDQNVRAILVNIFGGIARGDEVAHGLVEA
                     RKEQVRHVPMVVRIVGTNAEEAAQILAASGDPDIQAATTLDDAVAKVVAIASAPAATG
                     VGA"
     gene            2344..>3297
                     /locus_tag="VMZ33_01345"
     CDS             2344..>3297
                     /locus_tag="VMZ33_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HUR15904.1"
                     /translation="MSYTVTLIPGDGIGPELADATVTVLAATGIEFEWIRVDAGESQI
                     AKYGTPLPEAVLDSIRRTKVALKGPITTPVGEGFRSVNVTLRQALDLYANLRPARSMK
                     GVETRYEDVDLIIVRENTEDLYAGIEHRVGKDAAESIKIITRYASERIARFAFDYAVA
                     NRRKKVTAVHKANIMKLSDGLFLESCRTVAAEYDGRVEFEDRIVDNMCMQLVQKPDLY
                     DVLVLPNLYGDIVSDLAAGLVGGLGVAPGANIGVDAAVFEPVHGSAPKYAGKDVANPT
                     ALILSGALMLRHLGEVDAAEQVERAVREVIAEGQTVPRDLGG"
BASE COUNT          532 a         1104 c         1104 g          557 t
ORIGIN      
        1 gtcgcgttca tccatccgcg atcgtgccac ggcgtgctgg tcgaattgat cgaggctgtc
       61 ggcggtccgg catgggcagc gctgggaatg gagccgtccc ccagttgacc gactggtgag
      121 ccgccgcgcg cccccgtgtg acgggccgga ctatgagggc cggacgcccg cctcttggag
      181 cagcttcggt atctcgctcg gggcctgggc cacacggacg ccggcagcct cgagcgcggc
      241 cactttactc tccgccgttc cccgattccc gctgatgatc gcgccggcgt ggcccatccg
      301 acgccctggt ggcgcggtgc ggccggcgat gaacgcagcc atcggtgtcg cggtcatgtt
      361 gtcgcgtatc cagtcagcgg cgcgctcctc agcgtcgccg ccaatctctc cgatgagcac
      421 tacggcgtct gtgtcggggt cgtcgcggaa gagcttcagg atgtcgacga agttcgatcc
      481 gttgatcggg tcgccgccga tgccgacgca cgtcgactgg ccgatccccg cttccgtaag
      541 cgcctcgacg acctcgtaag tcaacgtccc tgagcggctt acgacgccga cgcgacccgg
      601 cctgtggatg cttcccggga tgatgccgac cttggcctga ccgaccgacg tcgcaccggg
      661 gcagttcggg ccgacgagcc gagcaccggc ctcgtcgacg accgggacga cgcgcagcat
      721 gtccagggcc gggatgtgct cggtgatgca gaagattgtc ttgattcccg cgccgacggc
      781 ttcgagaatc gcgtccggcg cgcctggcgc cggcacatag atgacgctcg tgttggcacc
      841 cgcctcagca actgcctcgg ccaccgtgtc gaacaccggg accggcttct cgagcgcgaa
      901 ctggccgccc ttccccggcg tcaccccggc gacgatgttc gtgccgtact cctgcatctg
      961 cagggcatgg aacgcgcctt cgcggccggt gatgccctgg acgacgaggc gcgtctcact
     1021 gttgacgagg atgctcacgc gccgactcca gtggcggctg gagcagaggc gatggcgacg
     1081 accttcgcca cggcgtcgtc gagtgtggtt gcggcttgga tgtccggatc gcccgacgcg
     1141 gccaggatct gtgcggcctc ctcggcgttg gtgccaacga tgcggacgac catgggcacg
     1201 tggcgcacct gttccttgcg cgcctccacg agcccatgcg cgacctcgtc cccgcgagcg
     1261 atgccgccga agatgttcac gaggatcgcc cggacgttct ggtccgaaag gatcagccgc
     1321 atcgccgccg ctaccttgtc ggccctggcg ccgccaccga tatcaaggaa gttggccggc
     1381 tggccgcccg cgagcttgac gaggtccatc gtcgtcatcg ccaggccggc gccgttgacc
     1441 atgcagccga tcgtcccctc gaggcggatg aacgacaggc cctgctcgcg cgccgcgatg
     1501 tctaccggat cctcctcgtc gaggtcgcgc atcgcctcaa tgtcggggtg gcgatgcagc
     1561 gccgagtcgt caatcgtgat cttggcgtcc aggcactgaa gggtttccgt gccgtctgcg
     1621 gcacgcacaa tcgccagagg gttgatctcg acgaggtccg cgtcgtcctc gaccatgacc
     1681 gtgtggaggc ccttggcgat ggccacgaac tccttgaggt gcgcaccaag accgagttga
     1741 aaggccagcc cgcgcgcctg atagtcgagc agcccaaggt gggcatgggc atggctgtag
     1801 gtaatcgcgt caggacgctc ctcggcgacg acctcgatct cgacaccgcc ctcggctgag
     1861 gccatgaaca gcgcccggcg cgcggcccgg tcaaggacca cagcgaggta atactccttg
     1921 acgatgtccg cggcgggcgc cacgagcacg cgacggacgg tcgtcccctt gatatccatc
     1981 cccaggacct ggctcgcgac ccgctctgcc tcgtccgcgc ttgaggccag ctttacgccg
     2041 ccggccttgc cgcgtccacc gacgaggacc tgggccttga tgacgacccg tccgcacccg
     2101 gcggcgaggt aaccctcagc ctgctcgcga gcctcggccg cagtcgacgc aacggcgtat
     2161 tcgggcacgg gcagaccggc ggccgagagc agccgcttgg cgtggtactc ctgaagcttc
     2221 acgacgagga aggttagtcg caagcgcagg gggacgcgcc agaaccttgt ggccgtgcct
     2281 gaatacactg tgactcccgc acccatggcg ctgtgaccgg agggcgcgcg agaggcaggt
     2341 ttcttgagct acaccgtcac gctgatccct ggggatggga tcggtccaga gctggccgac
     2401 gcaacagtca ccgtgttggc cgccaccgga atcgagttcg aatggattcg ggtcgatgcc
     2461 ggtgaatcgc agatcgccaa gtacggcacg ccgctgcccg aagcggtcct ggattcgatc
     2521 cgccggacca aggtcgcatt gaagggcccg atcaccacgc cggtggggga gggcttccgg
     2581 tccgtgaacg tcacgctccg ccaggcgctc gatctctacg cgaaccttcg gccggcacgc
     2641 tcgatgaagg gcgtcgagac gcgctacgag gacgtggatt tgatcatcgt ccgcgagaac
     2701 actgaggacc tatacgcggg catcgagcac cgggtcggca aggacgccgc ggagagcatc
     2761 aagatcatca cccggtacgc gtcagagcga atcgcgcggt tcgcgttcga ctacgcggtc
     2821 gccaaccggc gaaagaaggt cacggcggtg cacaaggcga acatcatgaa gctgtctgac
     2881 ggcctgttcc tggagtcgtg tcgcacggtg gcggctgagt acgacggccg cgtcgagttc
     2941 gaggaccgca tcgtcgacaa catgtgcatg cagctcgtcc agaagccgga cctctacgac
     3001 gttctggtgc tgcccaacct gtacggcgac atcgtgtcag acctcgcagc gggactcgtt
     3061 ggtggtctgg gcgttgctcc aggggcgaac atcggggttg atgcggccgt gttcgagccg
     3121 gtccacggtt ctgcgccgaa gtacgccggc aaggatgtcg ccaatcccac cgccctgatc
     3181 ctgtccggag cgttgatgct gcgccacctc ggcgaggttg atgccgcgga gcaggttgag
     3241 cgcgccgtgc gcgaggtcat cgctgaaggt cagacagtcc cacgcgacct gggcggc
//
LOCUS       DATLCO010000052         4068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2297155, whole genome shotgun sequence.
ACCESSION   DATLCO010000052 DATLCO010000000
VERSION     DATLCO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297155"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1180
                     /locus_tag="VMZ33_01350"
     CDS             257..1180
                     /locus_tag="VMZ33_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUR15905.1"
                     /translation="MLLTLLGIAAAGFAAQLIDGSLGMGYGVSSTTLLLVLGLTPAIA
                     SASVHLAEIGTSAVSGASHWRLGNVDMPVLFLLGIPGGVGAFLGAVVLSNLSLDAARP
                     WVSVVLLILGGVILFRFLHARRTRRAPAALHDQTKRADNRITGWRTWLLTPLGLAGGF
                     LDASGGGGWGPVTTSTLMASGRLKPRTIIGTVSGSEFIVSLAASAGFLLALGTAQIRW
                     DIVAMMLIGGSIAAPVSAWLVRSFDDRALGTAVGAVIILLNVDRILLMFGVDTAIVTL
                     VRVLVIAASIAIIGALIARGRASRAAQPEPA"
     gene            1309..2247
                     /locus_tag="VMZ33_01355"
     CDS             1309..2247
                     /locus_tag="VMZ33_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUR15906.1"
                     /translation="MELLLIVLAVGFAAQLIDGSLGMGYGVLSTSLLLAAGIVPAVAS
                     ATVHIAKVGTAVASGAAHWRFGNVDGKVALLLGIPGALGGYLGAVVLSNLSLNFARPY
                     VSGILLVLGLVIIGRFLHGRRKRRLAAEAAEAADETGNSSGFEPPKPQRPAWLLAPLG
                     LIGGFVDASGGGGWGPVTTSTLMASGRLSPRTTIGTVNAAELLVALSASAGFLQELGS
                     EGVRWDIVGAMLIGGVLAAIPAAWLVRHINERTLGIAVGVLILVLSIDSVLSLLQPPE
                     QLLLIFRVVGVVVTVVLLGLALFRARSESNEDSEPA"
     gene            2276..3499
                     /locus_tag="VMZ33_01360"
     CDS             2276..3499
                     /locus_tag="VMZ33_01360"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HUR15907.1"
                     /translation="MFYVSRGNIPPKRHTQHRAPDGSLYSEELFGMEGFTGRSSLMYH
                     LVPPTRVQRIERVRDIRIEAIDDGVHRHRLVKTSQTPGAGNVVEARVPLFFNNDVVIG
                     VIRPTEDLPVNAFYRNGEGDEMLFVHEGEGTFESNFGPMRYGPGDYIVIPIGTTWRLA
                     QDAGSDHRLMYIESPSEIVPPKRYRNDWGQLLEHSPFSQRDLRVPAEAPPRSESGAFV
                     IWIRVRGRITAYHYLHHPFDVVGWDGYLYPYIFNISDFEPLTGRVHQPPPVHQTFAGR
                     NYVVCSFVPRMFDYHPLAIPAPYNHSNINSDEVIYYVAGNFMSRRGVEISSFTLHPAG
                     IPHGPHPGTVEASIGKARTEELAVMVDTFHPLNLTREAARLEDESYPYSWLPPENAAE
                     HADALSARGPEAFPD"
     gene            3513..4061
                     /locus_tag="VMZ33_01365"
     CDS             3513..4061
                     /locus_tag="VMZ33_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HUR15908.1"
                     /translation="MGDIQPGVDPSADPTETGSDMLDGSDVMGRLNDVERRLNAHAER
                     EKPAGLTDPDPDGTERWEAGQVWGHMAEFVGYWSGELERVITTYDGEAVAFGRTKEDA
                     GRITGIEMGLGAAIPDLMGRVRDSIAELRRLLAGLTSAEWNTVGRHPTRGELDAEAIV
                     ERFIVAHLEEHADQLDTLTAHD"
BASE COUNT          656 a         1352 c         1322 g          738 t
ORIGIN      
        1 cagggagctg acggaccccg cggatgcctg gcaatcactc aaccggcacc gcgaaaccat
       61 gggtgggcaa ccgacttagg cgcgaccgtc gcctggcttc tctggcagcc ctcggacctt
      121 caacaaggtc atcggcctca caccgtaccg actgaggctt gacgcgcgca cgtctgcgcg
      181 gcattattgc gcccttaagt cgactaataa cataggaaac ctgggaatac aggagtattc
      241 cgttcgggag gaccagatgc ttctcacact tctcggaatt gccgcggcag ggttcgcagc
      301 gcagctcatc gacggctcgc tcggcatggg ctacggcgtg tcctcgacca ccctcctgct
      361 ggtgctcggg ctcaccccgg ccatcgcatc ggccagcgtg catcttgccg agatcgggac
      421 ttcggccgtc tcgggcgcgt cgcactggcg gctcgggaac gtagacatgc ctgtgctgtt
      481 cctgctcgga attccgggcg gcgtcggcgc cttccttggc gccgtcgttc tctccaacct
      541 atccctggac gcggcccgac cgtgggtgtc cgtggtattg ctcatcctgg gcggagtgat
      601 cctctttcgg ttcctccatg cccggcgaac tcgtcgtgcc ccggcggccc tgcacgatca
      661 gacgaagcgg gcggacaacc gcataaccgg ctggcgcacc tggctgctca ctcccctggg
      721 tctggcaggc gggttcctcg acgcgtccgg aggcggcggc tggggaccag ttacgacgtc
      781 cacgttgatg gccagcggtc gcctcaagcc gcggacgatc atcggcacag tcagcggctc
      841 ggagttcatc gtctcgctgg cggccagtgc cggctttctc cttgcgctgg gcactgcgca
      901 gatccgctgg gacatcgtcg ccatgatgct cattggcggg tcgatcgccg cgccggtgtc
      961 ggcctggctc gtgaggagct tcgacgatcg tgcgctgggc acggccgttg gcgcggtgat
     1021 catcctgctc aacgtcgatc ggatcctgct catgttcggt gtcgacacag caatcgtcac
     1081 gctcgtccgt gtcctggtca tcgcagcttc catcgccatc attggcgccc tcatcgctcg
     1141 cggccgtgcg tcgcgagctg cgcagcccga gcccgcatga gtttgcgagg tatccacgcc
     1201 ccaagcgttt gacagccacg gccggctggc cgaagattcc tcctcaattc ccacctcttt
     1261 agtcgggatt tccgcggcca ctcgcaccgc gtcggaggag aacgtttctt ggagctgttg
     1321 ctcatcgtgc tcgcggtcgg ctttgccgcg cagctgatcg atggatcgct cggcatgggc
     1381 tatggggtcc tgtcgacgag cctgctcctc gccgccggaa tcgtgccagc cgtcgcctcg
     1441 gcgacggttc acatagccaa ggtcgggact gcggtcgcct cgggtgcagc gcactggcgc
     1501 tttggaaatg tcgacggcaa ggtcgcgctg ctcctgggga taccgggcgc gctcggtggc
     1561 tacctcggcg ccgtggttct gagcaatctc agcctgaact tcgcgcggcc ttacgtgtcg
     1621 gggatcctgc tcgtgctcgg cctggtgatc attgggcgat tcctgcacgg gcggcgcaag
     1681 cgacgactgg cggctgaggc ggccgaagct gctgacgaga ccggtaactc ttctgggttt
     1741 gaaccgccca agccacagcg cccggcctgg cttctggccc cactcgggct cattggcggg
     1801 ttcgtcgacg cgagcggagg cggagggtgg ggccccgtaa cgacgtcgac gctgatggcg
     1861 tcgggccggc tctcgccacg aacgacgatc ggcacggtca acgccgcaga gttgctcgtc
     1921 gccctgtcgg cgagtgccgg ctttcttcag gagctgggct ccgagggcgt ccgttgggac
     1981 atcgtcggcg ccatgctgat cggcggcgtg cttgccgcga tccccgccgc gtggctggtt
     2041 cggcacatca acgagcgcac gctgggcatc gcggtcggcg tactgatcct ggtcttgagc
     2101 atagatagcg tcctgagcct gctgcagccg ccggagcagc tcctgctgat cttccgagtc
     2161 gtgggcgtcg tggtgaccgt cgtcctcctc ggactggcgc tattccgcgc gcgaagcgag
     2221 tcgaacgagg actccgaacc ggcctaacgt cggcggcgac atagaattcg cggcggtgtt
     2281 ctacgtcagc cgcggcaaca tcccgccaaa gaggcatacg caacaccgcg cgcccgatgg
     2341 ttctctctac tccgaggagc tcttcggcat ggagggcttc accggccgat cgtcactcat
     2401 gtaccacctg gtgccgccaa cgcgcgtcca gcggatcgag cgggtgcgcg acatcaggat
     2461 tgaggccatc gacgacggcg tgcatcggca ccgcctcgtc aagacaagtc agacgccggg
     2521 tgccggaaac gtcgtcgagg cacgcgtgcc gctgttcttc aacaacgacg tcgtcatcgg
     2581 cgtcatccgg ccgactgagg atttgcccgt aaacgccttc taccggaacg gagaaggtga
     2641 cgagatgctg ttcgtccatg agggcgaggg cacgttcgag agcaacttcg ggccgatgcg
     2701 ctacgggccc ggcgattaca tcgtcatccc gatcggaacg acctggcggc tggcccagga
     2761 tgccgggtcg gaccaccggc tcatgtacat cgagtcaccg tcggagatcg ttccgccgaa
     2821 gcgctaccgc aatgactggg gccagctgct ggagcactcc cctttttcgc aacgcgacct
     2881 tcgcgtgccc gccgaggcac cgccgcgtag cgaaagcggc gccttcgtga tctggatccg
     2941 cgtccgtggc cgaatcacgg catatcacta cttgcaccat cccttcgacg ttgtcggctg
     3001 ggacggctac ctctacccgt acattttcaa catcagcgac tttgagccgc tgaccggccg
     3061 cgtccaccag ccgccgccgg tgcatcagac ctttgccgga cgcaactacg tggtgtgctc
     3121 gttcgtgccg cgcatgttcg actaccaccc gctggcgatc cccgcgccgt acaaccattc
     3181 gaacatcaac agcgacgagg tcatctacta cgtcgctggc aacttcatga gccgccgcgg
     3241 cgtggagatc agctcgttca cgttgcaccc ggctggcatc ccgcacggac ctcatccggg
     3301 aacggttgag gcatcgatcg ggaaggcacg caccgaagag ctggcggtca tggtggacac
     3361 cttccacccg ctgaacctca cgcgcgaggc ggcgcgcctc gaggacgagt cctacccgta
     3421 ttcgtggctg ccgccggaaa atgccgccga gcacgccgac gcgctgtccg cccgcggccc
     3481 ggaggcattc cccgactgat gaggtgcgac tgatgggcga catacagccc ggcgtggacc
     3541 cgagcgccga tccaacggaa accggcagcg acatgctcga cggctcggac gtcatggggc
     3601 ggctgaacga cgtcgagcgc cgtctgaatg cccacgctga gcgcgagaag ccggccggcc
     3661 tcactgaccc tgaccccgac ggcacggagc gttgggaggc cggacaggtt tggggccaca
     3721 tggccgagtt cgtcggctat tggagtggcg agcttgagcg cgtcatcaca acgtatgacg
     3781 gcgaggcggt cgcgttcgga cgaaccaagg aggacgctgg gcgcatcaca gggatagaga
     3841 tgggcctggg cgcggcgatc ccggatctga tgggccgtgt ccgagactcc atcgccgagc
     3901 tgaggcgctt gctggcgggc ctgacgtcgg ccgaatggaa caccgtgggt cgccacccga
     3961 cgcggggcga gctcgacgcc gaagccatcg tggagcggtt catcgtcgct cacctcgaag
     4021 aacacgccga ccagctggac acgctcacag cgcacgactg acgcggta
//
LOCUS       DATLCO010000053         5049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_807464, whole genome shotgun sequence.
ACCESSION   DATLCO010000053 DATLCO010000000
VERSION     DATLCO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807464"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1679
                     /locus_tag="VMZ33_01370"
     CDS             195..1679
                     /locus_tag="VMZ33_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HUR15909.1"
                     /translation="MARLGRAAWRTLTSVKFAVLQITVLSVAGVIGTILDQIPAFALH
                     DPAAYAEQLATLHAKYDGAAILGVHLGPGMVSLFDTLGFFRVFSAPWFILLLTLLVVA
                     IVVCTLDRTPDLWHKARLVKINQAAPFFDLRLSERARFTNADDAAADELRKVLRAKHY
                     NVREEIEQVAEADTHAGADATPTPIRYVYGDRNQYMKLTTLFTHLGLILFLAGGAVTT
                     AMGYETVVFLGQGETAPVQAVGTQGNLLVKNIFFEAPRDAAGNFTDFRTDLAVYRDGQ
                     EVARKVIRVNDPLEVDGFVFHQNTFGPAEDLTIRDPNGRLVWDGPVLLAGELGGHPQG
                     FMTVPGSDIGLLLVLDETADGIPLLAVTGLGQTANPEESNILFIHALGLGGQTTPPST
                     VGYTIAWDAAASYTGMVIKNDPGAPIIWIAYMSLIIGLVLTFYFPRRRVWARLHGDHL
                     EMAFLGDRYVNAEREFGEVLDELSTRLRNRPERRLQPSAGVGRA"
     gene            1746..4022
                     /locus_tag="VMZ33_01375"
     CDS             1746..4022
                     /locus_tag="VMZ33_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HUR15910.1"
                     /translation="MVSELGERTVAHPNGHAAEGNGSGAGRVQGRGPVSVTTSAGAGL
                     SGRQNAINTIANHRVAKTQDYLEQAGADIYGQYVFGEDAQRQYLAKPIFEALRRTIAG
                     TEPFDQLIVDAVAHGVKEWAMARGATHYTHWFVPMTGSTAEKHDSFLTPSGEARTIAE
                     FSGKNLLQGEPDASSFPSGGIRATFEARGYTAWDVTSPIFLQVEPNGVTLTIPTAYVS
                     FTGEALDYKIPLLRSQDALGKQALRVLRWFGNDAATRVFTNIGPEQEYFLVDRRLAEQ
                     RPDLLLTGRTLFGAPSPKGQELEDQYFGPIRERILAFMMDLDRELWRLGIPAKTRHNE
                     VAPCQFEMAPVFEATSVGSDHNMLVMATMRKLAHQHGLMLLIHEKPFAGINGSGKHNN
                     WSMATDAGENLLDPGKDPHANAQFLAFLLAVIRGVDVHADLLRASIADAGNDHRLGAN
                     EAPPAIVSIFLGSQLEDVIDQLVAGPASASKDGGSLDLGVSSLPPLPRDATDRNRTSP
                     FAFTGNKFEFRAVGSSAPIYWPQTVLNTSVADSLEQLADELETMSPKDFDGLGAILSG
                     LAREHKKVIFGGNGYSPEWHAEAERRGLPNNKTTVDALPALATAKAKSVFSRFGVLSE
                     RELGARVEIAWERYVKVSNIEANAALDIARTMILPASLRYLAQVYAVSSASRAAESLC
                     TRLGELVDRLSVAIDELEHAQHAAHEAGWVQEEARAFVEKVIPAQMALREAADELETV
                     VADDLWPLPKYRELLFQY"
     gene            complement(4076..4918)
                     /locus_tag="VMZ33_01380"
     CDS             complement(4076..4918)
                     /locus_tag="VMZ33_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR15911.1"
                     /translation="MVLRRENDQRERWAVILGASSGFGAAAARALAHAGFGIVGVHLD
                     LRGTIAAAHSVRDEIEAIGVPVAFHNVNAADEEKRRGVIEATKQLFDERRALGKDPFV
                     CVMLHSLAFGTTLSYVTGTTEEREVSQKQLEMTADVMAHSLVYWTRDLFHAGMLADGS
                     RVFAMTSEGATKAVPTYGPVSAAKAALEAHVRQLALELIMHGVTVNSIRAGVTDTPAL
                     RRIPGHEELRSVAEARNPGGRLTRPEDVADALVALSSPLLYWMTGNTIGVDGGELMGA
                     RKVG"
BASE COUNT          872 a         1745 c         1582 g          850 t
ORIGIN      
        1 agggctcgaa gacatcgacg acctgttgac cgacttcgag cacggcctga aggccgcacg
       61 caccccggcc acaacccggt cggcctgacg cccgcgcccg cgccaggcgc aggcgagcag
      121 catcgaacac tccacgggac actgacatcg ccaccatcgc cctccccggc cgtcgcaccg
      181 agagcctgaa tccgttggcc cggctaggcc gtgccgcgtg gcggacgctg accagcgtca
      241 agttcgccgt actccagatc accgtcctgt ccgtggcggg cgtcatcggc acgatcctcg
      301 atcagatccc agccttcgcc ctgcacgacc cggcggctta cgccgagcag ctcgcgacgc
      361 tccatgcgaa gtacgacggg gccgcgattc tcggcgtcca cctcggtccg ggcatggtca
      421 gtctcttcga cacactcggc ttcttccgcg tcttctcagc gccgtggttc atccttctgc
      481 tgaccctgct ggtcgtcgca atcgtggtct gcacgctcga tcggacgccc gatctctggc
      541 acaaggcccg cctcgtcaag atcaatcagg cggcgccctt cttcgacctc aggctgagcg
      601 agcgcgcccg gttcaccaac gccgacgacg ccgcggccga cgagctgcgg aaggtgctgc
      661 gcgccaagca ctacaacgtt cgcgaggaga tcgaacaggt tgcagaggcc gatacacacg
      721 cgggtgccga tgccacaccg accccaatcc gctacgtcta cggcgaccgc aaccagtaca
      781 tgaagctgac cactctcttc acgcaccttg ggctgatcct gttccttgcc ggtggtgcgg
      841 tgacgactgc gatgggctac gagacggtcg tgttcctcgg gcagggcgag acggcgcctg
      901 ttcaggccgt ggggacacag ggaaacctgc tcgtgaagaa catcttcttc gaggcgccgc
      961 gcgacgccgc cggcaacttc accgacttcc gcaccgacct cgccgtctat cgcgacggcc
     1021 aggaggtagc gcgaaaggtc attcgcgtga acgacccgct cgaggtcgac ggcttcgtct
     1081 tccaccagaa cacatttggc cccgcagagg acctgaccat ccgcgacccc aacgggcgcc
     1141 tcgtgtggga cggaccggtt cttctggccg gtgagctggg cggacacccg cagggtttca
     1201 tgaccgtgcc cggatcggac atcggcctgc tgctggtcct cgatgagacg gcggacggga
     1261 tcccgctgct ggccgtgacg ggtcttgggc agactgccaa tccggaggag agcaacatcc
     1321 tgttcatcca cgccctgggt ctcggtggcc agacaacgcc accgtccacg gtcggctaca
     1381 ccatcgcgtg ggatgccgcc gcctcgtaca ctgggatggt catcaagaac gatcccggtg
     1441 cgccgatcat ctggatcgcc tacatgtcgc tgatcatcgg cctcgtgctt accttctatt
     1501 ttccccggcg ccgagtctgg gcgcgcctcc acggcgatca cctcgagatg gccttcctgg
     1561 gcgaccgcta cgtcaacgcc gagcgcgagt tcggcgaggt gcttgacgag ctctcgaccc
     1621 gactacgtaa ccgtcccgag cgtcgtttgc aaccatcggc gggtgtcggc cgggcctaaa
     1681 ctccatcagg ccgtgcgact ccaactacgt gtggcgctgg cctgcgtcag gagggactac
     1741 cgacgatggt cagtgagctg ggggagcgga ccgtcgccca cccgaacggt cacgcggcag
     1801 agggcaatgg cagtggcgcg ggacgcgtcc agggaagagg acccgtatcc gtgacgacct
     1861 ctgcgggggc gggcctgtcc ggtcgacaga acgccatcaa cacgatcgcc aaccaccgtg
     1921 tggccaagac gcaggactac ctcgaacagg ccggcgccga catctacggc cagtacgtct
     1981 tcggtgagga cgcgcagcgc cagtacctcg ccaagccgat cttcgaggcg ctccgccgca
     2041 ccatcgccgg cacggagccc ttcgaccagc tcatcgttga tgccgtggcc cacggcgtga
     2101 aggaatgggc catggcgcgt ggcgccaccc actacacgca ttggttcgtg cccatgaccg
     2161 gctccacggc cgagaagcac gattcgttcc tcacgccctc cggcgaggca cgcaccattg
     2221 ccgagttctc cggcaagaac ctgttgcagg gcgagcccga cgcctcgtcc ttcccgtccg
     2281 gtggcatccg ggccacgttc gaagcgcgcg gctacaccgc gtgggacgtc accagcccga
     2341 tcttcctcca ggtagagccg aacggcgtca ccctcacgat tccgactgcc tacgtctcct
     2401 tcaccggcga ggcgctcgac tacaagatcc cgctgctgcg ctcacaggac gcgctgggca
     2461 agcaagcgct gcgagtgctg cgctggttcg gcaacgatgc ggccacgcgt gtcttcacga
     2521 acatcgggcc ggagcaggag tatttcctgg tcgaccgtcg tctggctgag cagcggcccg
     2581 acttgttgct caccggtcgc acgctcttcg gcgcgccgtc acccaagggt caggaactgg
     2641 aagaccagta cttcggcccc atccgcgagc gcatcctcgc tttcatgatg gatctcgacc
     2701 gcgagctgtg gcgcctcggc attccggcca agacgcgcca caatgaagtc gcgccgtgtc
     2761 agtttgagat ggcgccagtg ttcgaggcta cctccgtcgg ctccgaccac aacatgctcg
     2821 tcatggcgac gatgcgcaag ctcgctcacc agcacggcct gatgctgctc atccacgaga
     2881 agccgttcgc cggcatcaat ggatcgggca agcacaacaa ctggtcgatg gccaccgacg
     2941 ccggcgagaa cctgcttgat ccgggcaagg acccacatgc gaacgcccag ttcctggcat
     3001 tcctgctggc ggtcatccgc ggggtcgacg tccacgccga cctgctgcgt gcctcgatcg
     3061 ccgatgctgg caacgaccat cggttgggtg cgaacgaggc accgccggcg atcgtgtcga
     3121 ttttcctggg ctcgcagctc gaggacgtga tcgaccaact ggtcgcggga cccgcctcgg
     3181 cgtccaagga cggcgggagc ctcgacctcg gcgtctcctc gctgccaccc ctgccacgcg
     3241 atgccaccga tcgcaaccgg acctcgccct ttgccttcac cggtaacaag ttcgagttcc
     3301 gcgccgtcgg ctcgtcggcg ccgatctact ggccgcagac ggtcctgaac acctctgtcg
     3361 ccgactcgct cgagcaactg gccgacgagc tcgagacgat gagtccgaag gattttgacg
     3421 ggctcggcgc catcctgtcg ggcctggcac gggagcacaa gaaagtcatc tttggcggga
     3481 atggctactc gcccgaatgg cacgccgagg ccgagcgccg cggcctgccg aacaacaaga
     3541 ccaccgttga tgcgctgcct gctctcgcga cggcgaaggc caagtcggtc ttctcgcggt
     3601 tcggcgtcct ttctgagcgc gagctcggcg cccgcgtcga gatcgcgtgg gagcgctacg
     3661 tcaaggtcag caacatcgag gcgaacgcag cactcgacat cgcgcggacg atgattctgc
     3721 cggcgtcact gcgttacctc gcacaggtgt acgccgtttc aagcgcgtcg cgcgctgccg
     3781 aatcgctatg caccaggttg ggtgagctgg tcgatcggtt gagcgtggcg atcgacgagc
     3841 tggagcacgc gcaacatgcg gcgcacgaag cgggctgggt ccaggaggag gcccgcgcct
     3901 ttgtcgaaaa ggtcattcca gcgcagatgg cgctgcgcga ggctgccgac gagctggaaa
     3961 cggtggtcgc cgacgacctg tggccactcc ccaagtaccg ggagctgctg ttccagtatt
     4021 gaaggctcct ggcgcacaac gagccgccgc tcatcggcta cgaccaggtg gtcagctaac
     4081 cgacctttcg agcgcccatg agctcgccgc cgtccacgcc aatcgtgttg ccggtcatcc
     4141 agtagagcag cggcgacgac aatgcgacca atgcgtcggc gacatcctca ggccgggtca
     4201 agcggccacc ggggttacgt gcctcggcga ccgaccggag ctcctcgtgg ccggggatgc
     4261 ggcgcagcgc cggcgtgtcg gtcacaccgg ctcggatcga gttcactgtg acgccgtgca
     4321 tgatcagctc cagcgcgagc tgtcggacgt gggcctccag ggcggccttc gcagcgctga
     4381 ccgggccgta ggtcggtacc gccttggtcg caccttcgct ggtcatcgcg aaaacgcggc
     4441 tgccgtcggc cagcatccca gcatggaaga ggtcacgtgt ccagtagacg agcgaatgcg
     4501 ccatgacatc ggcggtcatc tcgagctgct tctggctgac ttcacgctct tcggtcgtgc
     4561 ccgtcacgta tgacagcgtc gtgccgaagg cgagcgagtg gagcatcacg cagacgaagg
     4621 gatccttgcc cagcgcgcgg cgctcgtcga agagctgctt cgtcgcctcg atgacgccgc
     4681 gacgcttttc ctcgtcagcc gcgttgacgt tgtggaatgc cacgggcacc ccgatcgcct
     4741 cgatctcgtc acggacgctg tgggcggcag cgatcgtgcc ccggaggtcg aggtggacgc
     4801 cgacaatgcc gaagccagca tgagcaagtg ctcgcgccgc ggcggcgcca aacccactgc
     4861 tcgcccccag aatcacagcc cagcgttccc gctggtcgtt ttctctacgt agaaccacgg
     4921 ccgcagttta gccctccgcc accggcagtg gctgggaact caacggagcg caccagccaa
     4981 caccacaggt tggaacacac ggctggcggc tctgcttttg gggcgctgaa ggggctcgtc
     5041 tatactcgc
//
LOCUS       DATLCO010000054         3426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10464703, whole genome shotgun sequence.
ACCESSION   DATLCO010000054 DATLCO010000000
VERSION     DATLCO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10464703"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VMZ33_01385"
     CDS             complement(<1..900)
                     /locus_tag="VMZ33_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HUR15912.1"
                     /translation="MTRNPDWHKRDSAWRERTLSGATARSPERRTRFSTISDVEIERL
                     YSPLDWSLDGSGAGGPTAVDHLGDPLRQGAGQWSEFDPLRDIGYPGEAPFTRGIHPTG
                     YRTRLWTMRMFAGFGAAEDTNERFHQLLRAGQTGLSIAYDMPTLYGYDTDDAEAEGEF
                     GTCGVAVSSLADMEVLLDGLPLDRISTSMTINSPAAPIWAMYIAAAEKRGFPRATLEG
                     TTQNDILKEFVAQKEFLFPPEPSIRLVTDTIEFGTRELPKWNTISISGYHIREAGSTA
                     VQELAFTIADGMAYVEEAIRRGLR"
     gene            complement(1240..2019)
                     /locus_tag="VMZ33_01390"
     CDS             complement(1240..2019)
                     /locus_tag="VMZ33_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HUR15913.1"
                     /translation="MPTVRFRGNPYQVLGVRSDASNATIKRRWRELAREHHPDRAAGN
                     REEAARLTTRMARINAAYDLLRDPSRRQSFDSSAEGRLAREEDGGRGRRADFEDETVY
                     DPQTSPGPPPAQRTTPVTARFDTTSSFRPRNARLGEQRTAALRGQPPMGRARRDDADL
                     RASTPTGPVVTRRGKRAEPPPTLQEARETTLAFGRYHGYTLGEVELLEPAYLEWIGRT
                     ITRDRDLVTKARVILTDLDERGGDDKNRPPAPGFPSGATAG"
     gene            2078..3166
                     /locus_tag="VMZ33_01395"
     CDS             2078..3166
                     /locus_tag="VMZ33_01395"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HUR15914.1"
                     /translation="MKLPFQPPLEPMLAKISADLPQGDGWLFEPKWDGFRAIVFRDGD
                     ETYIQSRDLKPLDRYFPELAPALRQNLPDRCVVDGEIVIPGEGGLEFESLLLRIHPAA
                     SRVKMLSEQTPASFVGWDLLALGDEDLRAVPQVERRAVIERELRGTQAPVHLTPATRD
                     VDLARDWFNRFEGAGLDGVVAKPLAAVYQPGKRAMLKVKHARTADCVVAGFRWHKNGP
                     GTHIGSLLLGLYDDTGSLHHVGITSSFSWDRRAELVSELEPLRTNAITNHPWGEWAEW
                     ASQGQADVSGQRMPGATSRWNRGKDLSWEPLRIERVAEVAYDHLQGDRFRHATTFARW
                     RPDKQPQDCRYDQLEETAAFELSKIFGG"
BASE COUNT          554 a         1140 c         1147 g          585 t
ORIGIN      
        1 gcgcaaccca cggcggatgg cctcctcgac ataggccatg ccgtcggcaa tcgtgaaggc
       61 gagctcctgc accgccgtcg atccggcctc gcgaatgtgg taaccagaga tcgagatcgt
      121 gttccacttg gggagctcgc gagtgccgaa ttcgatggtg tcggtaacga ggcggatcga
      181 cggctcgggc gggaagagga actccttctg cgccacgaac tccttcagga tgtcgttctg
      241 cgtggtcccc tcgagagtcg cccgcgggaa gccgcgcttc tcggccgccg cgatgtacat
      301 cgcccagatc ggcgccgctg gcgagttgat ggtcatcgac gtcgagatgc gatcgagcgg
      361 caagccgtcg agcagcacct ccatgtcggc gagcgacgaa accgcgacgc cgcacgtgcc
      421 gaactcacct tcggcctccg cgtcgtcggt gtcgtacccg taaagcgtcg gcatgtcgta
      481 ggcgatcgag agccccgtct ggccggcccg cagcagctgg tggaagcgct cgttcgtgtc
      541 ctcggccgct ccgaagccgg caaacatgcg catcgtccac aggcgggtcc gataaccggt
      601 ggggtgaatt ccgcgcgtga atggcgcctc gcccggatag ccgatgtccc ggaggggatc
      661 gaactccgac cattggcccg ccccttgccg aagcggatcg ccgaggtggt cgaccgccgt
      721 cggcccgccc gcacccgacc catcaaggga ccaatcgagc ggcgaataga ggcgctcgat
      781 ctcgacgtcg ctgatggtcg agaagcgtgt cctgcgctcg ggtgagcggg ccgtcgcgcc
      841 actcagcgtc cgctcgcgcc aggcgctgtc gcgcttatgc caatcgggat tgcgggtcac
      901 gtttcgagcg tagcactggt tgataccaca gctggtttgg cgaatttcag gcgctgtgac
      961 gcgaaagcgg ctaaatggtg cgtggccgca cttccttttc gtttggtcgg ataggtaccg
     1021 aggcgcgata caacggcccg cgcgagcagc ctaatttggc agcgtcaaac cggggttggc
     1081 cactcaggga tgacatccgg ggcgtcgatc accgggttag gcgaagttgc ggtccgaaca
     1141 acgtgccgtg ccaacgcgta actgcgtgta accgcctttg cgtagcaacg agtcgagtaa
     1201 ctgggtgttg ccggccccgg agcggaatag ttcctggagt catccggccg tcgcgccact
     1261 cgggaaaccg ggggccggcg gccggttttt gtcatcccct ccgcgctcgt cgagatccgt
     1321 caggatgacg cgcgccttcg ttaccaggtc gcggtcgcgg gtgatggtcc ggccgatcca
     1381 ctcgaggtac gccggctcca gcagctcaac ctcaccgagc gtgtagccgt ggtagcggcc
     1441 aaaggcgagc gtcgtctccc ttgcctcctg cagcgttggc ggcggctcag cacgctttcc
     1501 gcgtcgggtc acgacgggtc ccgtgggcgt gctcgcgcgc aggtcggcat cgtcgcgacg
     1561 cgcccggccc atcggcggct gtccgcgcaa ggccgcggtc cgttgctcac cgagtcgcgc
     1621 gttccgtggc ctgaacgacg atgtggtgtc gaaacgcgcc gtcaccggtg tcgtccgctg
     1681 cgctggtggc ggtccgggcg acgtctgtgg gtcgtagaca gtctcgtcct cgaagtcggc
     1741 cctgcgaccg cggccgccgt cctcttctcg ggcaagccgg ccctccgccg agctgtcgaa
     1801 gctctgtcgc cgcgacgggt cgcgcaggag gtcgtaagca gcgttgatgc gcgccatccg
     1861 tgtcgtgagc cgggcggcct cctcgcgatt gcccgccgcg cggtcggggt gatgctcacg
     1921 ggcaagctcc cgccaacgtc gcttgatggt cgcgttggac gcgtccgatc ggacgcccag
     1981 aacctggtac ggatttccgc gaaaacgaac ggtgggcatt caacctccga gcctaccatc
     2041 cggcccgctt gcgctcgcct cgctagagtc acggccaatg aaactgccct tccagccgcc
     2101 gctcgagccg atgctcgcca agatctctgc cgatcttccg cagggtgatg gctggctctt
     2161 cgaaccgaag tgggacggct tccgggcaat cgtcttccgc gacggcgacg agacctacat
     2221 ccagagccgc gacctcaagc cactggatcg ctacttcccc gagctggcgc cagccctgag
     2281 gcagaacctc cccgaccgct gcgtcgttga cggcgagatc gtcatccctg gcgagggcgg
     2341 tctggagttc gagtcgctgc tgctgcgcat ccacccggcc gcgtcgcgcg tgaagatgct
     2401 gtccgagcag accccggcga gcttcgtcgg ctgggacctg cttgcactgg gcgacgaaga
     2461 cctgcgcgcc gttccgcagg tagagcgacg cgccgtcatc gagcgtgagc tacgcggcac
     2521 tcaagccccg gtccacctca cccccgcgac gcgcgacgtg gacttggcac gcgactggtt
     2581 caaccgtttc gagggcgccg gactcgacgg cgtcgttgcc aagccgctgg ccgctgtcta
     2641 ccagcccggg aaacgggcga tgctcaaggt caagcacgcg cgaaccgccg attgcgtcgt
     2701 ggccggcttt cgatggcaca agaacgggcc gggcacgcac attgggtcgc tgctgctcgg
     2761 gctgtacgac gacaccggct cgctgcacca cgtcggcatc acctcgtcgt tttcgtggga
     2821 tcgccgggcc gagctcgtca gcgagctgga acccctgcgc acgaacgcca tcacgaacca
     2881 cccttggggc gagtgggccg agtgggccag tcagggccag gcggacgtca gcggacagcg
     2941 catgcccggc gccacgagcc gttggaaccg cggcaaggac ctgtcgtggg agcccttgcg
     3001 catcgagcgc gtggccgagg ttgcctacga ccacctgcag ggcgaccggt tcaggcacgc
     3061 cacaacgttt gcccgatggc ggcctgataa gcaaccccaa gactgtcgct acgaccagtt
     3121 ggaagaaacg gcggcgtttg agctgtcgaa gatctttgga ggctagggcc gacaggcgga
     3181 gatttcaccc tttgcaaggg cagtcgcgcg ggtaaactcc gtctcgtcca aactcggcgg
     3241 cccgtcactg acgcccggct ggatccggcg gggttcgcgg agcaaattag taggagggat
     3301 acatggatca gtcacagccg cagtggagcc caccaccgca gcaaccaacc ggttggggtg
     3361 gcccaggcta cggcggcccc ccggtacggc cgaccggtgt gacgctcggc tcaatcttct
     3421 tgatcg
//
LOCUS       DATLCO010000055         5428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5996002, whole genome shotgun sequence.
ACCESSION   DATLCO010000055 DATLCO010000000
VERSION     DATLCO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5996002"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VMZ33_01400"
     CDS             complement(<1..409)
                     /locus_tag="VMZ33_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUR15915.1"
                     /translation="MTVHSDSLPQPRKRRKNVRPTEGRAPRPSATTPSSVTGERGPTL
                     RAGWRVIAAKEFADHVTSVRFLVLTIVLSLAAAAAVYSTAGALRNVASEASGSPSLFL
                     LLFTRGVGDIPAFTSFLGFLGPLLGIAFGFDAIN"
     gene            complement(406..1440)
                     /locus_tag="VMZ33_01405"
     CDS             complement(406..1440)
                     /locus_tag="VMZ33_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR15916.1"
                     /translation="MADAEPPVDQELIPAVATASKPDKVPAAIRIRGLTKRYGDFTAV
                     DQLDLNVRHGEVYGLLGPNGAGKTTTILMLLGLSEPTSGAARVLDMDPARNPVEVKRR
                     VGYLPDNVGFYGGMTGRQNLRYTARLNRVDRDVVEDRIEGLLGRVGLTDAADKSVEKY
                     SRGMKQRLGLADVLVKDPSIIILDEPTTAIDPAGVEDVLALIRELADDGAAILLASHL
                     LHQVQQVCDRVGIFVSGRLVASGRMDKLAGELGTGPISIEVAAPPPLDAVMNAARAIR
                     GVTSVRRDDRDARMVLIDADHDVRAEVATALVAAGLPPSHLRRRGDELDEIYRRYFAA
                     GEEASPGAAA"
     gene            complement(1427..2611)
                     /locus_tag="VMZ33_01410"
     CDS             complement(1427..2611)
                     /locus_tag="VMZ33_01410"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HUR15917.1"
                     /translation="MRALRSFLAALLIAAGVVPAALPAAVFAQGPLEMTTDFPSVVAD
                     AGTDVQFPVTVTTDTPTRVDLTVTSQPDGWTVRFQGGGSTINAVSTHPTAEQPDDNVA
                     EFTAEVSVPEAITPGSNEVQIDGRTSAGTTFSLVLTVTVEETEPGSVTLETDFPSLEG
                     PTSATFSFNLTLTNDTNTQQTFGLETDAPAGWTVTARPSGEDQAATAVVDAGSNEQIS
                     VTVDPALAADAGQYPIIVRAVSDAATAEAALGVEITGSFSLDMFTPDDRLNARVTAGG
                     STVLNFIVENTGTAPIANLTFSESAPQGWAVTFSPETITSLAPGASSRETIAATITAD
                     GDSLAGDYVLTLNANSEDADGSLEIRTTVETSPIGYVIGIAILVIVAIGLFVVFQRYG
                     RR"
     gene            complement(2734..4230)
                     /locus_tag="VMZ33_01415"
     CDS             complement(2734..4230)
                     /locus_tag="VMZ33_01415"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HUR15918.1"
                     /translation="MQFRTISRLDGEQVDAVIVPVFKEGNAAASTPDDLKELAEWVAK
                     ETGGGKLFTATTHLDRGNGSQNGRRVVVVAAGSHDDYDVQRAWQVIAAGMRSLWNSTA
                     ERVAVVIETHRLDNAAAVQSAVEGVSFAMWRPESHRTSDADRQLPPVRELLIVSSEFE
                     EFPDNDEAVARGQAVGDAVNWARTLANEPGNHMTPKHVAEEARQLASQTGLEVQVIGE
                     DEARSLGMGSFLSVARGSDEEAQVVVLRHRGRDAEGYDLALVGKGITFDSGGISLKDP
                     TDMHLMKYDMSGAAAVLAVAGVVARLKLPMNLLVVAMCTENLPGGHATKPGDVFTSMS
                     GKTVEVTNTDAEGRLVLIDGITYAQREGAARVVDVATLTGAVAVALGRHYTGLFGRPE
                     GFVDAVRRAGELAGERLWPMPLSDEYRDEMKSEIADLRNSAGRMGGAILGAAFVDAAV
                     EEQTEWAHLDIASTAWSEEERLYTPKGPQGSSVRTLVQLAGSIAARNS"
     gene            4353..4943
                     /locus_tag="VMZ33_01420"
     CDS             4353..4943
                     /locus_tag="VMZ33_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HUR15919.1"
                     /translation="MTRRIFSFDAPDRFVVGAIGQPGQRTFYLQAAKGAQVVSVALEK
                     AQVAVLAERLASLLLALRQGGVAIEEDVTSATALLDQPVVEQFRVGTLTLGWDGERQR
                     IVIEAREITDEDEDEEEDDDEGEAEEPEEALALGANDLEDGPDLVRVQLDATAALAFA
                     SGAVAVIQAGRPPCPQCGAPLDPIGHFCPRRNGYQN"
     gene            5025..>5428
                     /locus_tag="VMZ33_01425"
     CDS             5025..>5428
                     /locus_tag="VMZ33_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15920.1"
                     /translation="MLGRLRDASNATFLCKIGIDEDDEDIYAVYKPTRGERPLDDFPR
                     RTLGQREVAAHALSQATGWHIVPPTIWRDGPAGLGMVQLWMEPDPESDVVTMVVERDP
                     RLRTMAVFDALANNADRKGGHILPMTDGHVYGV"
BASE COUNT          911 a         1797 c         1824 g          896 t
ORIGIN      
        1 cgttgattgc atcgaacccg aacgcgatgc ccagcagcgg tccgaggaaa cccaggaacg
       61 acgtgaatgc cgggatgtcg cccacgcctc gcgtgaagag cagcaggaag agcgacgggg
      121 agcccgacgc ctccgaggcg acgttgcgta gtgcgcccgc cgtcgagtag acagcggccg
      181 cggcggccag cgaaaggacg atggtcagca caaggaagcg cacgctcgtg acgtgatcgg
      241 cgaattcctt ggcggcgatg acccgccaac cagcgcgcag cgtcggccca cgttcccctg
      301 tgacggaaga aggcgtcgtg gcggaaggac gcggcgccct gccctcggtc gggcgtacgt
      361 tcttgcgccg cttgcgcggc tgcggaagtg agtcgctgtg cacggtcatg ccgccgctcc
      421 tggagacgct tcctccccgg ccgcgaagta gcggcgataa atctcgtcta gctcgtcgcc
      481 acggcgccgg aggtgggacg gcggcaggcc ggccgcgaca agtgcagtcg ccacctccgc
      541 gcgaacatcg tgatcggcgt ctatgagcac catccgggcg tcacggtcgt cgcgacgaac
      601 cgacgtcacc cctcgaattg cgcgtgctgc attcatcacc gcatcgagcg gtggcggcgc
      661 ggccacctcg atgctgatcg gacctgtgcc aagctcgccg gcgagcttgt ccatccgccc
      721 gctggcgacg agcctgccgc tgacgaagat cccgacgcgg tcgcacacct gttgcacctg
      781 gtgcaggagg tggctcgcga gcaggatcgc ggcgccgtca tcggccagct cccggatcag
      841 cgccagcaca tcctcgacgc cggccgggtc gatggcagtc gtcggctcgt cgaggatgat
      901 gatcgacggg tccttgacca gaacgtcggc aaggccgagc cgctgcttca tgccacgcga
      961 atacttctcg accgatttgt cggctgcgtc ggtgaggccc acgcgcccga gcaggccctc
     1021 gatgcgatcc tcgacgacat ccctgtcgac gcggttcagc cgcgccgtgt agcgcaggtt
     1081 ctgccgcccg gtcatgccgc cgtagaagcc cacgttgtcg ggcaggtagc cgacgcgtcg
     1141 cttgacctcc accgggttac gcgccgggtc catgtccagc acccttgccg ccccggaagt
     1201 aggctcggac aggccgagca gcatcaaaat cgtcgtcgtc ttgcccgcac cgttgggccc
     1261 gagcaggccg tagacctccc catggcgcac gttcaggtcg agctggtcga ccgccgtgaa
     1321 gtcgccgtat ctcttggtca gcccgcggat gcgaatcgcc gctggaacct tgtccggctt
     1381 actcgccgtc gctacggccg ggatcagctc ctggtcgacc ggcggctcag cgtcggccat
     1441 agcgctggaa gacaacgaac aggccgattg ccacgatgac caggatcgcg atgccgatga
     1501 cgtagccgat cggagaagtt tcgacggtcg tgcggatctc gaggctgccg tcggcatctt
     1561 cggagttcgc attgagggtg agcacgtagt cgccggcgag ggagtcaccg tcggccgtga
     1621 tcgttgccgc aatggtctcg cgactcgacg cgccaggtgc gagtgacgtg atcgtctccg
     1681 ggctgaaagt gacagcccag ccctggggag cactctcgct gaacgtcaga ttggcaatcg
     1741 gcgccgtgcc cgtgttctcg acgatgaagt tgaggacagt ggagccgccg gctgtgacgc
     1801 gtgcattcag acgatcgtcg ggggtgaaca tgtcaaggct gaagctgccc gtgatctcaa
     1861 ccccgagcgc agcttcagcc gtggccgcat cactgaccgc gcgaacaatg atggggtact
     1921 gaccggcatc ggcagcgagc gccggatcga ccgtgacgct gatctgctca ttcgagccgg
     1981 cgtccaccac cgcggtcgca gcctggtcct cgccggaggg acgggccgta actgtccagc
     2041 cagccggggc atcggtctcc agcccgaacg tctgctgcgt gttggtgtcg ttggtaagcg
     2101 tcaggttgaa ggagaaggtg gcgctggtcg ggccttcgag gctcggaaaa tctgtttcaa
     2161 gggtgacgga ccccggctcc gtctcctcga cggtcactgt cagaaccagg ctgaaggtcg
     2221 tgccggcgga cgtgcggcca tcgatctgca cctcgttgga acctggcgtg atcgcctccg
     2281 gaacggagac ctctgcggtg aactcggcaa cgttgtcgtc cggctgctca gccgtcggat
     2341 gcgtcgagac ggcattgatg gttgagccac cgccctggaa ccggaccgtc cagccatcag
     2401 gctgagacgt cacggtgagg tcaacgcgcg tcggcgtgtc ggtggtgaca gtgaccggga
     2461 actgcacatc ggtgcccgcg tcggcaacga ccgacgggaa gtcggtggtc atctcgaggg
     2521 gtccctgggc aaacactgcg gcaggcaagg ctgccggcac aacgcccgct gcgatgagca
     2581 gcgcggcaag gaaggaacgc aaagcgcgca tctctatccg agctctccta ttcgtttggc
     2641 gcacacggtt acctgattga atgccgggca cctgtgacgg tgctgagagt taccgctgat
     2701 tccgctgagc gtgacggccc ggcggagagc ggcttacgag ttacgcgccg cgatgctacc
     2761 agcgagctgg acgagcgtcc gcaccgaaga accctgcgga cccttcggcg tgtacaggcg
     2821 ctcttcctct gaccaggccg tgctggcgat atcgagatgt gcccactcgg tctgctcctc
     2881 caccgcggcg tcgacgaacg ccgcgcccag gatcgcacca cccatgcggc cggcgctgtt
     2941 ccggagatcg gcgatctcgc tcttcatctc gtcgcggtac tcgtctgaga ggggcatggg
     3001 ccagagtcgc tcgccggcaa gctcaccagc gcggcgtacg gcgtcaacga agccctcggg
     3061 tcggccgaac agacccgtgt agtggcgccc gagcgccact gcgactgcgc ccgtgagcgt
     3121 ggctacgtcc acgacgcggg ccgcgccctc gcgctgtgcg tacgtgatgc cgtcgatgag
     3181 caccagccgg ccctcggcgt cggtgttggt gacctcgacc gtcttgccgc tcatgctcgt
     3241 gaagacgtcg ccgggcttgg tggcgtgacc gccgggcagg ttttcggtgc acatcgcgac
     3301 gacgaggagg ttcatcggca gcttcaaccg agcgaccact ccggctaccg ccaggaccgc
     3361 ggccgcaccg ctcatgtcgt atttcatgag gtgcatgtcg gtcgggtcct tgagcgagat
     3421 gccgcccgag tcaaaggtga tgcccttgcc aaccagcgca aggtcgtatc cctcggcgtc
     3481 acggccgcga tggcgcagga cgacgacctg cgcctcttcg tcgctgccgc gggccacgga
     3541 caggaacgag cccatcccca gcgagcgtgc ctcgtcctcg ccgattacct gcacctccag
     3601 gccggtctga ctggcgagct ggcgcgcctc ctcagcgacg tgcttgggcg tcatgtggtt
     3661 gcccggttcg ttggccagcg ttcgggccca gttcacggca tcgccgacgg cctggccgcg
     3721 cgccaccgcc tcgtcgttgt ccgggaactc ctcgaactcc gaagagacga tgagcagctc
     3781 ccggacgggt ggcagctggc ggtccgcgtc agacgtccga tgggactcgg gccgccacat
     3841 ggcaaagctc acgccttcga ccgcgctctg cacggcggcc gcgttgtcga gccggtgcgt
     3901 ctcgatgact acggcaacgc gctccgcggt cgagttccag agcgaccgca ttccggctgc
     3961 gatgacctgc cacgcacgct ggacgtcgta gtcgtcatgg gagcccgccg ccacgacgac
     4021 gactctgcgc ccattctgcg agccgttgcc gcgatcaagg tgggtcgtgg cggtgaacag
     4081 cttgccgccg cccgtctcct tggcgaccca ctcggcgagc tccttcaggt cgtcgggcgt
     4141 ggatgccgct gcgttgcctt ccttgaaaac aggcacgatg acggcatcaa cctgctcgcc
     4201 atcaagacgc gagatcgtgc ggaactgcaa gcgaaccttc ctcctctgat tggtacacgg
     4261 cgcggcgaga agactaaatc ccattcagac ttagggtccg caagacccgc gcagcagtgg
     4321 gccgaaggcc actgcggagg caacataggc atatgacccg ccgaatcttc agtttcgacg
     4381 cacccgatcg cttcgtcgtt ggcgcaatcg ggcagcccgg ccaacgcacc ttttacctgc
     4441 aggccgcaaa gggcgcgcag gtggtgtctg tcgccctcga gaaggcacag gttgccgtgc
     4501 tcgcagagcg gctggcgagc ctgctgctgg ccttgaggca gggtggcgtc gccatcgagg
     4561 aggacgtcac cagtgccacg gccctgctcg accagccggt agtcgagcaa tttcgcgtag
     4621 gcacgctgac cctcggctgg gacggggagc ggcaacggat cgtgatcgag gctcgtgaga
     4681 tcaccgacga ggacgaagac gaggaggagg acgacgacga gggtgaggcc gaagagcccg
     4741 aggaggctct ggcactgggc gccaacgatc ttgaggacgg gcccgatctg gtgcgggtgc
     4801 aactagacgc gacggccgcc ctggcctttg cgagcggcgc cgtcgccgtg atccaggccg
     4861 ggcgaccgcc gtgtccccag tgtggcgcgc cactcgatcc gatcggccac ttctgtccac
     4921 gacgcaacgg ctaccagaac tgacgccgcg ccgagcttcc tgagctgaca cgctcaaacg
     4981 gcggtgaagc cgcgctggat ctgctgcgca cctgtgatct ggaggtgctt ggtcgcctga
     5041 gggacgcgag caacgccacg ttcctgtgca agatcggcat cgacgaggac gacgaggaca
     5101 tctacgccgt ctacaagccg acccgcggcg agcggccgct cgatgacttc ccgcggcgca
     5161 ccctgggaca gcgcgaggtc gcggcacacg ccctgtccca ggccactggg tggcacatcg
     5221 ttccgccgac gatctggcgc gacgggcctg ccggcctcgg gatggttcag ctctggatgg
     5281 agcccgaccc cgagtcggac gtggtgacga tggtcgtcga gcgcgaccca cgactgcgaa
     5341 ccatggcggt cttcgacgcg ctggccaaca acgctgaccg caagggtggc cacatcctgc
     5401 cgatgaccga cggccacgtg tatggcgt
//
LOCUS       DATLCO010000056         7194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10467250, whole genome shotgun sequence.
ACCESSION   DATLCO010000056 DATLCO010000000
VERSION     DATLCO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10467250"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..927)
                     /locus_tag="VMZ33_01430"
     CDS             complement(7..927)
                     /locus_tag="VMZ33_01430"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HUR15921.1"
                     /translation="MTGAATRRMHEKEIDISVDLVRDLLVDQFPELSTRAISRVESPG
                     TDNTIFRIGDDIAARFPRIDWAVGDAEREHRWLPHLSEHLPVATPRPLGLGAPGHGYP
                     WRWLVVEWLPGQNAYDAAPKDTRRLAMDLAAYVEALHGIDATSGLPRAAPRARRGRPL
                     ETQDEAARSSLAALHHELDVPAATGAWNDALTAPKWTGRPTWIHGDLQATNLLLVDDT
                     LTAVIDYGGLGVGDPAADMIPAWALFDARDREVFKDAVGVDEATWARGKGWALSVGLI
                     ALPYYLHTNPVIVAWARQSIHAVLRDRYPR"
     gene            complement(924..1769)
                     /locus_tag="VMZ33_01435"
     CDS             complement(924..1769)
                     /locus_tag="VMZ33_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HUR15922.1"
                     /translation="MTRVAIVADSAGDLTAKQAQNAGISVVPLLVTFGEREYRAGVDL
                     SSEDFYAQLTAPGAPFPKTAAAAPGTFRETFERLLAEGADEIVYVGVGAKLSATLGSA
                     KVARDMLPDRTIRIVDSNSASMGAGLLALRASEMAAAGASSDEIAADLERRREGISLY
                     VVLETLEYLKRGGRISPARAAIGSVLSVKPIITILDGVVETADKPRTRGRARERLIEL
                     ITATSPERVAILHSQSDDVEAFATELAAATKVPRAEMLVNVIGASVGPHVGPGAFGAV
                     TLCAR"
     gene            complement(1773..3002)
                     /locus_tag="VMZ33_01440"
     CDS             complement(1773..3002)
                     /locus_tag="VMZ33_01440"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HUR15923.1"
                     /translation="MDSSNGQVFIVSAARTPIGKFGGSLADVPATTLGGIAIKAAVER
                     AGLPEGTPIDEVLMGQVLQAGVGQAPARQAALKAGLGEGTSATTINRVCGSGLKAIML
                     AAAEIRAGDAEIVVAGGMENMNLGPYLIPKARFGYRLGNAEMIDATVHDGLWCSTESC
                     HMGTHAERVAIKNQVSREDQDQFALESHQRALAAIKDGRFAEEIVPVPIKKGKETVDF
                     AVDEGPRPDSSLEALGKLKPVFALPDPDPDSTVGLPGGTVTAGNAPGITDGAAATVVA
                     SERAVERLGLTPLARIVGYAQAEVEPKWLFLAPVKGVQKLLARIEMPIEAFDLIEINE
                     AFAAQVLADGRELGFDWSRVNVNGGAIALGHPIGASGARIMATLLHELSRRGGRYGLA
                     TLCLGGGGSVAMAVERV"
     gene            3064..5115
                     /locus_tag="VMZ33_01445"
     CDS             3064..5115
                     /locus_tag="VMZ33_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR15924.1"
                     /translation="MPEETLTEAQLRERGLAGLAELARLDERTGSINAVAARALDLVG
                     SLLDADRAAVCAIDGDETIEWLAHRRMEQFIAASADLRPSDLLWARRPLETGRPELFD
                     RRAPSHERSALSDTADALGMAAFAIVPLRSGQEFTGILGLVWNDDPPELGADEELLTT
                     IGRLVALALGNTRLRDTLLARQAELDESEARYRSLFEQAPEPMLICDWDGAVLDFNRA
                     AMALYGVTGHRLLNHRLETLTDISLDRRRRLAAALHRSRREEVEARGRRRDGSEFPLV
                     LHLAVIAFRGEERLLVQLRDMTDQQRMQAELLQAQKMEALGQLISGVAHELNNPLSAI
                     IAFSQLLRSDERLPADLARDADLLVGEADRTRRIVQNLLDFARQRTPDRQPTSLPALV
                     ERTLELHAYALRAGRIETRVLMPTDLPDVWADSAQIQQVLLNLTLNAIQSIRASNPNG
                     TLTIAAHTEPRSDGPRFVRLRVEDDGPGVELRSRDHLFEPFFTTKDVGQGTGLGLSVS
                     YGIVAAHGGKLWYEPASVAGATFLLELPAAADIERTLPGKSGQLATAAANRGLGSTVL
                     VVDDERAIRETIRRALERAGHSVTAAGSGAEALEILRDRSVDLLLIDHRMSAMDGTEF
                     YGYAIAARPELTGRALMMTGDTLNPELLEFVRENSLSLIAKPFDIDDLTAEVDSALAP
                     A"
     gene            complement(5122..6000)
                     /locus_tag="VMZ33_01450"
     CDS             complement(5122..6000)
                     /locus_tag="VMZ33_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HUR15925.1"
                     /translation="MTERKVLVCGAGLMGHGIAQVMAAAGHRVWLYEPDAARAEAGRE
                     RIAGNLERAVAKGKISDGDRTAQLGRVSVATSLDEAASQAELVVEAIFEDEAVKQALF
                     AEVAAAAQPSTVLASNTSSISITRLAATTSAGRRARFVGMHFFSPVPVMPLIELIRGA
                     ETDDATEAYVRDVAAELGKQVIVSKDRPGFIVNRILMPYLGESMRAFEEGLGTAEDID
                     SGAKVGLNHPMGPLELADFIGLDVCLHIMEVLQEGFGAAHMAPPPILRQLVDAGHLGQ
                     KTGRGFYAYPREPKKG"
     gene            complement(6022..7005)
                     /gene="meaB"
                     /locus_tag="VMZ33_01455"
     CDS             complement(6022..7005)
                     /gene="meaB"
                     /locus_tag="VMZ33_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HUR15926.1"
                     /translation="MTPSLAERARSGDRRALARLLTHVENRTLAGESALRELYPHAGS
                     AHLVGVTGAPGSGKSTLVAALVGEARTSGRPVAVIAIDPSSPITGGAILGDRVRMQQH
                     ASDTDVFIRSMASRGHSGGLAAATIAAAAAFDAAGFQLVFIETVGTGQSEVEIAAIAD
                     TTLVVQAPEMGDEVQAIKAGLLEVADIVVVNKSDRPGADRAAAQLRGMLTVGAQHDRA
                     MGDRPRPKRPDVVLASAMTGAGVGELLAALDDRAAARRAADSDPFVALAAAHKRAEAQ
                     LSGIMADRLRTRLATPAHREAAAAVVARVARHEQDPYSAADELLAKLADNS"
     gene            complement(7002..>7194)
                     /locus_tag="VMZ33_01460"
     CDS             complement(7002..>7194)
                     /locus_tag="VMZ33_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15927.1"
                     /translation="LPKITAALREQGMDDVLVTAGGIIPDADIPQLREVGVARVFGPG
                     TTIREVAEFFRGNVARREG"
BASE COUNT         1135 a         2459 c         2398 g         1202 t
ORIGIN      
        1 gggcgattac cggggatacc tatcgcgcag gacagcatgg atcgactgcc tcgcccaagc
       61 aacgatgacg ggattggtgt gcaggtagta cgggagggca atcaatccga ccgacagcgc
      121 ccagccctta ccgcgtgccc atgtcgcctc gtcgacaccg accgcatcct tgaagacctc
      181 gcggtctcga gcgtcgaaca gcgcccaggc ggggatcatg tcggccgccg ggtcgccgac
      241 cccgagcccg ccgtagtcga tgactgcggt caatgtgtcg tccacgagga gaaggttcgt
      301 tgcctggaga tcgccgtgga tccaggtcgg ccggccagtc cactttgggg cggtcagtgc
      361 atcattccag gccccagtcg ccgctggaac gtcgagttcg tggtggagtg ccgccaggct
      421 ggatcgggcg gcctcgtctt gagtctccag cggacgccct ctacgggcgc gtggcgcagc
      481 gcgcggtagt ccgctcgtcg catcgatccc atgcaacgcc tccacgtacg cagcgagatc
      541 catagcgagt cgccgtgtgt ctttcggtgc tgcgtcgtag gcattctgcc cgggcagcca
      601 ctccacgacg agccagcgcc atggataacc atgtccgggc gcaccgagtc ccaggggtcg
      661 tggcgtcgcg acggggaggt gctcagaaag gtgcggcagc caacggtgct cgcgctcggc
      721 atctccgacc gcccaatcga ttcgcgggaa ccgggcagcg atgtcgtcgc cgatgcggaa
      781 gatggtgttg tctgtgcccg gcgattcaac ccgacttatg gcgcgcgtgc tcaactctgg
      841 gaactggtcg accagcagat cgcggacgag gtcgaccgag atatcgatct ctttttcgtg
      901 catgcggcgg gtggccgcgc cagtcacctc gcgcagagag tgacggcccc gaatgcgccc
      961 gggccgacgt gcgggccgac cgacgcgccg atgacgttca cgagcatctc ggcgcgcggc
     1021 accttcgtgg ccgcggccag ctccgtggca aatgcctcga cgtcgtcgct ctggctgtgg
     1081 aggatagcca cgcgctcggg gctggtcgcc gtgatcagct cgatcaagcg ctcgcgcgcc
     1141 cgacccctcg tccgtggctt gtccgcggtt tcgaccacgc cgtccaagat ggtgatgatg
     1201 ggtttgactg acagcacgct gccgatcgcc gcccgtgctg gcgaaatgcg cccaccacgc
     1261 ttcaggtatt ccagggtttc gagcacgacg tagaggctta tgccttcgcg gcgtcgctcg
     1321 aggtcggccg ctatctcatc actcgaggca cccgccgcgg ccatttctga cgcgcggagt
     1381 gccagcagac ccgcgcccat gctggcgctg ttggagtcga cgatcctgat cgtgcggtcg
     1441 ggcagcatgt cgcgagccac ctttgcgctg ccgagagtcg ccgatagctt ggccccgacg
     1501 ccgacgtaca cgatctcgtc ggctccttcg gcaagcagcc tctcgaaggt ctcccgaaac
     1561 gtgcccggtg ccgcagccgc ggtcttcgga aagggcgccc ccggcgccgt cagctgggca
     1621 tagaagtcct cgctcgacag atccacgccg gcccggtact cacgttcgcc gaacgtgacc
     1681 agcaggggta cgacgctgat gccggcgttc tgcgcctgct tggcggtgag gtcgcccgcc
     1741 gagtcggcga cgatcgcgac gcgggtcagg gattagaccc tttcgacggc catggcgact
     1801 gagccgccac cgccgaggca cagggttgcg aggccgtacc tgcctccgcg ccggctcagc
     1861 tcgtggagca gcgtggccat gatgcgtgcg ccactggcgc cgatgggatg tccgagcgcg
     1921 atggcgccac cattgacgtt gaccctcgac cagtcaaacc cgagctcgcg gccgtcggca
     1981 agcacctgtg ccgcgaaagc ctcgttgatc tcgatcaggt caaacgcctc gatcggcatc
     2041 tcgatgcgag caagcagctt ctggacgccc ttgacgggcg caaggaaaag ccacttcggc
     2101 tcgacctctg cctgggcgta gccgacgatc cgggcgagcg gtgtcagtcc caaacgttcc
     2161 acagcgcgct cactggcaac cacggtcgcg gcggcaccgt ccgtgatacc cggcgcattt
     2221 cccgcggtaa cagtgccgcc cggcaggccg acggtggagt cggggtctgg gtcgggcagc
     2281 gcaaagacgg gcttcagctt gcccagcgcc tcaaggctgc tgtccggacg cggcccttca
     2341 tcgaccgcga agtcgaccgt ctccttgccc ttcttgatcg gcacggggac gatctcttcg
     2401 gcaaagcggc cgtccttgat agcggcgagg gcccgctggt gggactccag tgcgaactgg
     2461 tcctggtctt cgcggctgac ctggttcttg atcgccacgc gctcggcgtg cgtgcccata
     2521 tggcacgact cagtggagca ccacaggcca tcgtggacgg tggcgtcgat catctcggcg
     2581 ttgcccaggc gatagccaaa ccgcgccttc gggatgagat acggcccgag gttcatgttc
     2641 tccataccgc cggccacgac gatctcggca tcaccggcgc gaatctcagc cgcggcgagc
     2701 atgatcgcct tgaggcccga accacagaca cggttgatcg tcgtcgcgct ggtcccctcc
     2761 cccaggccgg ccttcagggc tgcctggcgc gccggcgcct ggccgactcc cgcctgcagc
     2821 acctggccca tgaggacctc gtcgatgggc gtgccttcgg ggaggccggc gcgctcaacg
     2881 gcggccttga tcgcgatgcc gcccaacgtc gttgccggca cgtcggcgag cgatccgccg
     2941 aacttgccga tcggcgtccg tgccgccgaa acgatgaata cctgtccgtt gctgctgtcc
     3001 attgccattc cgtcgtcaat gttagtcggc gggaatacct actccctgac gtagcatctc
     3061 cacgtgcccg aggagacgct gaccgaggcg cagctcagag aacgtggcct ggcgggcttg
     3121 gccgagcttg cccgtcttga cgagcgcacc gggtcgatca acgcggtggc ggcgcgggcg
     3181 cttgacctcg tgggctcgct cctcgacgcc gaccgcgccg ccgtgtgtgc catcgacggc
     3241 gacgagacaa tcgagtggct ggcccatcgc cggatggagc aattcatcgc ggcttcggca
     3301 gacctgcgac cgagtgacct cctgtgggcg cgtcgcccgc tcgagacggg acgacccgag
     3361 ctcttcgacc gccgcgcgcc atcccacgaa cgaagtgccc tctccgacac cgcagacgcc
     3421 ctgggcatgg ccgcctttgc catcgtgcca ctccgatccg gccaggagtt caccgggatc
     3481 ctgggcctcg tctggaacga cgatccgccg gagctgggcg cggacgagga gctgctgacg
     3541 accatcggtc ggctggtggc gctggcgctg ggcaacacgc ggttacgcga cacattgctc
     3601 gcgcgccagg cggagctcga cgaatcggaa gcgcgctatc ggagcctctt tgagcaggcg
     3661 cccgagccga tgctcatctg cgactgggat ggcgccgtgc tcgacttcaa ccgcgccgcc
     3721 atggcgctct acggggtgac cgggcaccgc ctgctgaacc accgcctgga aacgctgacc
     3781 gacatcagcc tcgaccgccg tcgtcgtctc gcggctgcgc tccaccgctc gcgccgggag
     3841 gaggtcgagg cacgcgggcg gcgccgggac ggttccgagt tcccgctggt gctgcacctc
     3901 gcagtcatcg cgttccgcgg tgaggagcgg ctgctggtcc agctgcgcga catgaccgat
     3961 cagcaacgca tgcaagccga gctccttcag gcgcagaaga tggaggcgct cggccagctc
     4021 atcagcggcg tggcgcacga gctcaacaac ccgctgagcg cgatcatcgc gttctcgcag
     4081 ctgttgcggt ccgatgagcg cctgccggcg gacctggctc gggacgccga cctgctggtg
     4141 ggcgaggccg accgcacacg acggatcgtc cagaacctcc ttgactttgc ccgtcagcgc
     4201 acgcccgacc gacagccaac ctcactcccc gcactggtcg aacgaacgct ggagctgcat
     4261 gcgtacgcgc tacgggcggg ccgaatcgaa accagggtcc tgatgccgac cgatctgccc
     4321 gacgtctggg ccgactcagc ccagatacag caggtcctgc tgaatctgac cctgaatgcc
     4381 atccagtcga tcagggcgtc caacccgaac gggacgttga ccattgccgc gcataccgag
     4441 ccgcgctcgg acggcccacg attcgtccgt ctgcgtgtcg aggacgacgg ccctggtgtc
     4501 gaacttcggt cgcgcgacca cctctttgag ccgttcttta cgacaaagga tgtcggccag
     4561 ggcacgggtt tgggcctgtc cgtcagctac ggcatcgtcg ccgcgcacgg gggcaagctc
     4621 tggtacgagc cggcttcggt cgctggcgcg actttcctgt tggagctacc ggctgcggct
     4681 gacatcgaac gaactctccc tggcaagagt ggacagctgg cgacagcggc tgccaaccgg
     4741 ggcttgggca gcacggtcct cgtggtcgac gacgagcgcg cgatccgcga gaccattcgt
     4801 cgtgccctcg agcgtgccgg acattcggtg accgctgcgg gctccggggc ggaggcgttg
     4861 gaaatcctgc gtgatcggtc ggtcgatctc ctgctcatcg accatcgcat gagcgcgatg
     4921 gacgggacgg agttctatgg ctatgccatc gccgcacggc cggagctgac gggtcgggcg
     4981 ctcatgatga ccggcgacac gctcaacccc gagctgctgg aattcgtccg cgagaactcg
     5041 ctcagcctga tcgccaagcc gttcgacatc gacgacctga ctgcggaggt tgactccgcg
     5101 ctcgcacctg cctaggagcg gctacccctt cttgggctcg cgcgggtacg cgtagaagcc
     5161 gcgaccggtc ttctggccga gatggccggc gtcaaccagc tgacgcaaga tcgggggggg
     5221 cgccatgtgc gccgcgccga aaccctcctg gagaacctcc atgatgtgca ggcagacgtc
     5281 caggccgatg aagtcagcca gctcaagcgg gcccataggg tggttgaggc cgaccttggc
     5341 gccggagtcg atgtcctcgg ctgtacccag gccctcctcg aaggcacgca tcgattcgcc
     5401 gaggtagggc atcaagatcc gattgacgat gaagcccggc cggtccttgc tgacgatgac
     5461 ctgcttgccc aactcggcgg caacgtcacg gacataggcc tctgtcgcgt cgtccgtctc
     5521 ggcgccacgg atcagctcga tgagcggcat gacgggcacg gggctgaaga agtgcatgcc
     5581 cacgaacctg gcgcggcgcc cggcactggt ggtggcggcg agtcgcgtga tggagatcga
     5641 gctggtgttg ctggcgagga cggtgctggg ttgagcggcg gcggcaacct ccgcgaatag
     5701 cgcctgcttg acggcctcgt cttcgaagat ggcctcgacc acgagctcgg cctgagacgc
     5761 cgcctcatcg agcgaggttg ccacgctgac tcggccgagc tgagcggtcc gatccccgtc
     5821 gctgatcttg cccttggcca cggcgcgctc gagattgccg gctatccgtt ctcgcccggc
     5881 ctcagcgcgc gccgcatccg gttcgtagag ccacacacgg tggccggccg cggccattac
     5941 ctgggcgatg ccgtggccca tcaggcccgc gccgcacacg agcacctttc gttcggtcac
     6001 ggcgggaagc ctagcgcagc gtcagctgtt gtcggcgagc ttcgcgagca gctcgtcggc
     6061 agccgaatac gggtcctgct cgtgccgtgc gacgcgtgcc acgaccgccg ctgccgcctc
     6121 acggtgggcg ggagttgcca ggcgcgtgcg taatcggtcg gccatgattc ctgaaagttg
     6181 ggcttcggcg cgcttgtgtg cagcggccaa cgcgacgaac ggatctgaat ccgcggcacg
     6241 gcgggcggca gcgcgatcat caagtgcggc cagaagctcc ccaacgccgg cgccggtcat
     6301 cgccgacgcc agcacaacgt cgggtcgctt cggacgcgga cggtcgccca tggcccggtc
     6361 gtgctgggcg ccaacggtca gcatgccccg cagctgcgcg gcggcgcggt cggcgccggg
     6421 tcgatcggac ttgttgacaa caacgatgtc ggccacttcc agcagtcccg cctttatcgc
     6481 ctgtacctcg tcgcccattt ccggcgcctg gacgacgagc gtcgtatcgg cgatcgcagc
     6541 gatctcaacc tcgctctgcc cggtgccgac cgtctcgatg aagaccagct ggaacccggc
     6601 ggcgtcaaac gctgcggcag ccgcgatcgt ggccgcggct agcccgcccg agtggccacg
     6661 cgacgccatc gagcggatga acacatcggt gtcgctcgca tgttgctgca tgcgcacccg
     6721 gtcgccgagg atcgcacccc cggtgatcgg gctggatggg tcaattgcga tgacggcaac
     6781 cggtcggccg ctcgtgcgtg cctcacccac gagcgcggcg accaacgtgg acttgccgga
     6841 accgggcgct ccggtcaccc cgacgaggtg cgccgaaccg gcgtgtgggt aaagctcgcg
     6901 cagcgctgac tcgccggcca gggttcggtt ctcaacgtgg gttagcagcc gcgccagggc
     6961 gcgccgatca ccgcttcgag cccgttcagc gaggcttggc gtcacccctc gcgccgtgcg
     7021 acgttacctc gaaagaactc cgcgacctcg cggatcgtcg tccccgggcc gaagacgcgc
     7081 gcaacgccaa cttcgcgcag ctgtggaatg tccgcgtcgg ggatgatccc gccggccgtg
     7141 accagcacgt cgtccatgcc ctgttcgcgg agcgcggccg tgatcttggg aagt
//
LOCUS       DATLCO010000057         5425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_813568, whole genome shotgun sequence.
ACCESSION   DATLCO010000057 DATLCO010000000
VERSION     DATLCO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813568"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VMZ33_01465"
     CDS             complement(<1..644)
                     /locus_tag="VMZ33_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HUR15928.1"
                     /translation="MAPNDFTHLHVHSEFSLLDGLGRITELVEDAKNQGFDSLAITDH
                     GALYGAVAFYKACADAKIKPIIGVETYIARRSMSDREGKADAQPYHLILLAKDWTGYQ
                     NLCRIVTDAHVDGYYYKPRIDREYLAQHAEGLVGLSACLNGEVARAIETGDMDEAKRI
                     AGSYRDILGEGNFFFELQDHGLMEQRRLNEALLKLGPEMGIPLVATNDLHYVRR"
     gene            754..1185
                     /locus_tag="VMZ33_01470"
     CDS             754..1185
                     /locus_tag="VMZ33_01470"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HUR15929.1"
                     /translation="MNDGVGEVGDGSGEDYFERMIEQALDELPPRFRAQIANVAIVID
                     DVATPELLAATNAPGLFGVYQGIPRTAWGADHAAIANKITIFRRPIEAWYPAGPARAQ
                     AVRDTLFHEIAHHFGISDERLDELSRARRRRSQPRPPRPVP"
     gene            complement(1130..1933)
                     /locus_tag="VMZ33_01475"
     CDS             complement(1130..1933)
                     /locus_tag="VMZ33_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUR15930.1"
                     /translation="MSQSSPPAPESAETQAPTKGFEPRPPGRLKHWFIRYGLRFFVSC
                     YLRVRVTGLHNVPTDRPYMLCFSHPSWVDPMLLAAFWPDTPRVFIFGPKEEDMHAGRR
                     NKIIRWTHMAVPFTPSKTNLLDTTRRAVAVMKGGYVLALAGEGRLSDRDGEIVPLEDG
                     ASYFALRARVPILPMTIIGSRWLRFGKRIEMRAGPLIELGDYRADRTGMLELTRITQD
                     AMERLLVGVVDEGPPGPFGRWMTDLLAERPWLTEQAAGDAAATDDVEPG"
     gene            complement(1905..2969)
                     /locus_tag="VMZ33_01480"
     CDS             complement(1905..2969)
                     /locus_tag="VMZ33_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUR15931.1"
                     /translation="MPLDENQAREFNRAAQDAPRVLIVEDEPTQRGLLTRVLEREGYE
                     VLAYGDGEMGLRAISEHAPEMVLLDLSLPRMDGFEICRRLRADPLTATLPVIVITAHT
                     AIDDMVAALDAGADDFLAKPVQQAELLARIRSAMRLRRSITSLERATQIVAALANAVE
                     AKDIDLVHHCRWLAHHAARVGVQVGLRGDELEAVAYGALLHDVGKIGVPEHLLRKEGP
                     LSDDEWTLMRRHPEIGEKICRPLSASRAFAPIIRHHHERFNGRGYPDGLRGEQIPLGA
                     RIVAVADAYEAMVHGRPYQPAQPHSLAAEELLRKRGSQFDPDLVPIFLDELERDTAGI
                     APAVALPHVAALEPEFAAGA"
     gene            3185..4453
                     /locus_tag="VMZ33_01485"
     CDS             3185..4453
                     /locus_tag="VMZ33_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HUR15932.1"
                     /translation="MRLRTSISLVLTTMLLIAACGGGGATTRPSTASVAPSTAASTAP
                     STAPSAEPTDEPTTEPTTEPTTEPTTEPTTEPSASASASDWSVGVVTDVGTVDDKNFN
                     EYSYNGAVAGATDIGAGEVDVAVPADASEYASLIQNFVDTGHNIIVTVGFNLANDTAI
                     SALANPDVWYVGVDQAPCIDETGAPDPTFGCAGDLATLLPNFVGLQYAEDQAGYLAGI
                     VAASISENDTVGAIGGINLVPAVVRYLQGYELGAKSVNPDITVVTDYVSTSDFGVAFN
                     DPGAGKDFADLFIETNGPDVLFQVAGKTGNGVLESACAAGIHGIGVDVDQYLSLDAGN
                     NPAYECIVTSAEKHLATSVEATVTAIAAGSQTAGLSFFNAENDGIGISPEGSGAGLIT
                     AEIQTLIDDAIAGMIDGSVVTCPETCGTAE"
     gene            4550..>5425
                     /locus_tag="VMZ33_01490"
     CDS             4550..>5425
                     /locus_tag="VMZ33_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR15933.1"
                     /translation="MTTEGPVTIAPVEAVPPPPPVPALEMRGITKRYPGVVANDGINL
                     DVRPGEIHALLGENGAGKSTLMNILYGLAVPDEGEILLDGQPIKIGDPTDAIARGISM
                     VHQHFMLVPVLSVAENIILGDETMGRVGFLDRGEAHRRIAELAKRFGFDIDPEAKVGT
                     LSVGQQQRVEILKALYRDTRILVLDEPTAVLTPQETTEIFVLLRKLAESGRSIIFISH
                     KLYEVLEIADRITVIRRGKVVGERSPRDTSEEDLAELMVGRAVDLTADRGESTPGEVV
                     LKVAGLRVKDDRGHEV"
BASE COUNT          995 a         1780 c         1745 g          905 t
ORIGIN      
        1 ttgcgccgca cgtagtgcag gtcgttggtc gctaccagcg gaatgcccat ctccggcccg
       61 agcttcagca gagcctcgtt gagccggcgc tgctccatga gcccgtggtc ctggagctcg
      121 aagaagaaat tgccctcacc caggatgtcg cggtacgagc cagcgatgcg cttcgcttcg
      181 tccatgtcgc ccgtctcgat ggctcgggca acctcaccgt tcaggcaggc tgacagcccg
      241 acaaggccct cagcgtgctg cgccagatac tcgcgatcga tccgcggctt gtagtagtac
      301 ccgtcgacgt gcgcatcggt gacgatgcga cagaggttct ggtagcccgt ccagtccttg
      361 gcgagcagaa tcaggtggta cggctgggcg tcggccttgc cttcgcggtc ggacatcgaa
      421 cggcgcgcga tgtaggtctc gacgccgatg atcggcttga tcttggcgtc ggcgcacgcc
      481 ttgtagaacg ccacggcgcc gtacagcgcg ccgtggtcgg tgatcgcgag cgaatcgaac
      541 ccctggttct ttgcgtcttc gaccagctcg gtgatcctgc cgaggccgtc gagcaggctg
      601 aactccgaat ggacatggag gtgcgtgaag tcgttcgggg ccactagctc gaccgtaacc
      661 gtgaccgcac caacatcgct aggcagtcat ccacgaactt catccacaga ccgtccagta
      721 tagaacggct gttctaagcg ttacgatcgg ccgatgaacg acggagtggg cgaagtaggc
      781 gacggaagcg gcgaggatta cttcgagcgg atgatcgagc aggccctcga cgagctgccg
      841 ccccggttca gggcgcagat tgcgaacgtc gcgatagtca ttgacgacgt cgccacgcca
      901 gagctcctgg cggcgaccaa cgcacctggg ctattcggcg tctatcaggg cattccacgc
      961 acggcttggg gcgccgatca cgctgccatc gcgaacaaga tcacgatctt ccggcgaccg
     1021 atcgaggcgt ggtaccccgc cggtccggcg cgggcgcagg cagtgcgcga caccctgttt
     1081 cacgagatcg cacatcactt cggcatcagc gatgagaggc tggacgagct cagccgggct
     1141 cgacgtcgtc ggtcgcagcc gcgtcccccg cggcctgttc cgtgagccac ggccgctcgg
     1201 ccagcaggtc ggtcatccag cgcccgaacg gccccggcgg tccctcgtcc accacaccca
     1261 ccaggagcct ctccatcgcg tcctgggtga tcctcgtcaa ctccagcatg ccggtgcgat
     1321 ccgccctgta gtcaccgagc tcgatcaatg gcccggcgcg catctcgatc cgcttcccga
     1381 aacgcaacca gcgtgatccg atgatcgtca tcggcaggat cggcactctg gcgcgcagcg
     1441 cgaaataaga cgcgccatcc tcgagcggca cgatctcgcc atcgcggtcg gatagtcgac
     1501 cttccccggc cagagccaga acgtagccac ccttcatcac cgcgaccgcc ctacgggtcg
     1561 tgtcgaggag gttggtcttt gacggagtaa acggcacggc catgtgtgtc cagcggatga
     1621 tcttgttgcg acgcccggca tgcatgtcct cctccttcgg gccgaagatg aagacgcgcg
     1681 gtgtgtcggg ccagaaggcc gccaacagca ttgggtcgac ccagctcgga tggctgaagc
     1741 agagcatgta gggccgatcg gtcgggacgt tgtgcagccc tgttactcgg acccttagat
     1801 agcacgagac gaagaaccgc aggccgtagc ggatgaacca gtgcttgagc cggccgggcg
     1861 ggcgaggctc gaaccccttg gtgggcgctt gcgtctcggc cgactcaggc gccggcggcg
     1921 aactctggct caagggctgc gacatgcggc agtgcgactg ccggagcgat acctgccgtg
     1981 tctcgttcga gctcgtcaag aaagatcggc acgagatcgg gatcgaactg cgacccgcgc
     2041 ttgcgcagga gctcctcggc agcgagcgaa tgcggctgag cgggctggta tggacggccg
     2101 tggaccatcg cctcgtaggc atcggctaca gccacgatcc gcgccccgag cgggatctgt
     2161 tcgccgcgca gcccatctgg gtagccacgg ccgttgaagc gctcatggtg gtggcggatg
     2221 atcggcgcaa aggcgcgcga cgcgctcaac ggccggcaga tcttctcgcc aatctccgga
     2281 tggcggcgca tgagcgtcca ttcatcgtcg gaaagcgggc cctccttgcg cagcaggtgt
     2341 tccggcacgc cgatcttgcc gacgtcatgc agaagcgctc cataagcgac tgcctcgagc
     2401 tcgtcgcccc ggagcccgac ctgcacgccg acgcgcgctg cgtggtgcgc cagccagcgg
     2461 cagtggtgga cgaggtcgat gtccttggcc tcgaccgcat tggccagcgc ggcgacgatc
     2521 tgcgtagctc gctcgaggct ggtgatcgat cgacgcagac gcatggcgct gcggatgcgc
     2581 gccagcagct cagcctgctg gaccggcttg gccaggaagt cgtccgcgcc ggcatcgagg
     2641 gcggcgacca tgtcgtcgat cgcggtgtgc gccgtaatga cgataacggg caatgtcgcg
     2701 gtcagcggat cggcacgcag acggcgacag atctcgaaac cgtccatgcg cggcaagctg
     2761 aggtcgagca gcaccatctc aggcgcgtgc tccgagatgg cacggaggcc catctcgcca
     2821 tcgccgtacg caaggacttc gtagccctca cgctcgagca cgcgagtgag caaaccgcgt
     2881 tgcgtgggct cgtcctcgac gatcaatacc cgtggcgcgt cctgggctgc ccgattgaac
     2941 tcccgcgcct ggttctcgtc gagtggcata cccgccacgt gcacctccaa cctgcaatcg
     3001 cgacttccac agcaatgcgg acgccgaggc aggtccctcg cgggcacgaa cgggaacctt
     3061 ccgcgagtat ggcatgcgta cacgcctgtg ttgcgacggc tcggacggat gcgtagcatt
     3121 cgcgccgagg gtcgggggac caaaattgaa cgtgcctatg aagcacgacc agaggaggaa
     3181 ataaatgcga ctacgcacgt cgatcagcct cgtgctgacg accatgttgc tcatcgccgc
     3241 ctgcggcggc ggaggggcaa cgacgcgacc gtcaacagct tcggtcgcgc catcgaccgc
     3301 agcgagcaca gcaccgagca ccgctccgag cgctgagccc actgacgagc cgacgaccga
     3361 gccgaccacg gagccgacga ccgagccgac gacggagccg acgaccgagc cttcggcctc
     3421 tgccagtgca agcgactgga gcgtcggtgt cgtgaccgac gtcggtaccg tcgacgacaa
     3481 gaacttcaac gagtattcgt acaacggtgc tgttgcaggc gccactgaca ttggcgctgg
     3541 cgaagtggac gtcgctgttc ccgcggacgc gtccgagtac gcgtcgctca tccagaactt
     3601 cgttgatacc ggccacaaca tcatcgtcac tgtcggcttc aacctcgcca acgacaccgc
     3661 gatctccgcc ctggcgaacc ccgatgtctg gtacgtcggt gtggaccagg cgccatgcat
     3721 tgacgagacc ggcgctccgg atccgacctt cggctgcgcg ggcgacctcg ccacgctcct
     3781 gccgaacttc gtcggtctcc agtacgccga ggatcaggcc ggctaccttg ccggcatcgt
     3841 ggccgcgagc atcagcgaga acgacacagt cggcgcaatc ggtggcatca acctggtgcc
     3901 cgccgtcgtg cgctatctgc agggctacga gctcggcgcg aagtcggtca accccgacat
     3961 caccgtcgtg accgactacg tctcgacatc ggacttcggt gtcgcattca acgacccggg
     4021 cgcaggcaag gacttcgctg acctcttcat tgagaccaac gggcccgacg tcctgttcca
     4081 ggttgctgga aagaccggca acggcgtcct cgagtccgcg tgcgcggccg gcatccacgg
     4141 gatcggcgtc gacgtcgacc agtacctgtc cctcgatgcc gggaacaacc cggcctacga
     4201 gtgcatcgtg acctcggctg agaagcacct tgcgacttcg gtcgaggcga cggtcacggc
     4261 gattgccgcg ggcagtcaga ctgccggcct gtcgttcttc aacgcggaga acgatggcat
     4321 tggcatctca cccgagggca gcggcgctgg cctgatcacg gctgagatcc agacgttgat
     4381 cgacgatgcc atcgcgggca tgatcgacgg gtcagtcgtc acctgccccg agacctgcgg
     4441 cactgccgag taactcgcgg actggcaagt cattggagcg ggtcgcgtta tacgcggccc
     4501 gctctttttc tatcctgtag gtagtcgcca tcaggagggt gttgatccgg tgaccactga
     4561 ggggcccgtc accatcgcgc cggtcgaggc cgtcccgccg ccaccgccgg ttcctgcgct
     4621 ggaaatgcgc ggcatcacca agcgctatcc gggcgtcgtc gccaacgacg gcatcaacct
     4681 ggacgtgagg cccggcgaga tccatgcatt gctcggcgaa aacggcgcgg gcaagtcaac
     4741 cctgatgaac atcctctacg ggctggccgt tcccgacgag ggcgagattc tgctcgacgg
     4801 acaaccgatc aagatcggcg acccgacaga cgccatcgct cgcggcatca gcatggtcca
     4861 ccagcacttc atgctggtcc ctgtcctgtc ggtggctgag aacatcatcc ttggcgacga
     4921 aacgatgggc cgagtcgggt tcctcgatcg aggcgaagcc catcggcgga tagccgagct
     4981 tgcgaagaga ttcgggttcg acatcgatcc cgaagccaag gtcgggacgc tctccgtcgg
     5041 ccagcaacag cgcgtcgaga tcctgaaggc gctctaccgc gacactcgaa ttctggtcct
     5101 cgacgagccg acggccgtgc tcacaccgca ggagacgacc gagatcttcg tgctcctgcg
     5161 gaagctcgcc gaaagcggcc gcagcatcat cttcatcagc cacaagctct acgaagtgct
     5221 cgagatcgcg gaccggatca cagtcatccg acggggcaag gtggttggcg agcggtcacc
     5281 cagagacacg agcgaggaag acctggctga gctgatggtt ggccgtgcgg tcgacctgac
     5341 cgcggaccgc ggggaatcga cgccgggcga agtcgtcctg aaggtggcgg gcctgcgtgt
     5401 caaggacgac cgcgggcacg aggtc
//
LOCUS       DATLCO010000058         5489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_69416, whole genome shotgun sequence.
ACCESSION   DATLCO010000058 DATLCO010000000
VERSION     DATLCO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69416"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VMZ33_01495"
     CDS             complement(<1..513)
                     /locus_tag="VMZ33_01495"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HUR15934.1"
                     /translation="MDAVPGGALPSDVGQGIPDQPPSPVLIHDREFVWGARTYVLGII
                     NVTPDSFSGDGIMSRDTAVVQAAVEQARRMVDDGADILDIGGESTRPGHADVSSADEK
                     SRVIPVVRAVRAALPEVPISIDSTKADVAAAALEAGADVINDIWGTGASDAMAGLAAE
                     RNAPMIVMHNR"
     gene            complement(514..1749)
                     /locus_tag="VMZ33_01500"
     CDS             complement(514..1749)
                     /locus_tag="VMZ33_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HUR15935.1"
                     /translation="MRYQDTLARLRQRGRFGIRLGLGRTRALLRRMGDPQAGLPGALI
                     GGTNGKGSTQAMLASVLKHGGYRVGQTPKPHLVSYRERIVVDGSHIPINDFCDLIDEV
                     LAQADHVEGRHGPPTEFEVITAAAFTYFRRAKVDLAVVEVGMGGRLDATNVWSGGASA
                     ITNVGLDHMEYLGSTVEAIAREKAAIIKRGDAWAVTGAGHPALSVIWGRARRVHVPLV
                     ETSAPAQRIALGLLGAHQQANAAVAVGVLDGLDRMGFPTTAAVRAAGLAKAEWPGRLE
                     HINVRGVDVLLDGAHNPDGMRALAAALGELLPQLGSGEITLLIGILENHWQEGMLDPL
                     NSAAPGALLVATEVPDAPNSLPLARIDTAWAGRARRIEDTDKALEAALATARDAGATL
                     LVCGSLYLVGYVRAQLVGE"
     gene            complement(1775..2068)
                     /locus_tag="VMZ33_01505"
     CDS             complement(1775..2068)
                     /locus_tag="VMZ33_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15936.1"
                     /translation="MRRPADPPPGAGAMMPRIPFGLPNLRRNVCPHFYMTGPRGPIPI
                     TAPHLLKALMRQDMNVARCQLRGGVHLEQSYVGDICLSPRFNQCVFYEDELNV"
     gene            2484..3440
                     /locus_tag="VMZ33_01510"
     CDS             2484..3440
                     /locus_tag="VMZ33_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15937.1"
                     /translation="MLTVGASMALAGNAMREGPGSPTQPPGVQTPGAATLIQPAPDIT
                     QDASVDITGTLPPNLARIGESTLRVFVNGDQVRERDLPAEQQFVLDDVPLVEGHNEIT
                     VGLTVDGHSGAQSAPVSITSDSTAPEIQILRPEPGTIAYAGRETMRGRTEAGASIEIY
                     ARGSEQELPVSIGGDGRFEAVIVLAMGDNDFTLRSIDLAGNPTTVHHVITRQVTLASI
                     TLNSSIEEVALAGLPAAVDLTANVRDEHGGKLEGVDVVFSLSPPTRATTTYRATTRNG
                     VARWSNVSIPDSPTSVGTWLATVLVILPSGEELREDVAFVVH"
     gene            3473..3670
                     /locus_tag="VMZ33_01515"
     CDS             3473..3670
                     /locus_tag="VMZ33_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15938.1"
                     /translation="MTKIGPAGAGEALDQQPRRSSPVSGDFLRSMPQKPTPPITERWK
                     FTQVPQLVADRPQRKHAVESA"
     gene            complement(3767..>5489)
                     /locus_tag="VMZ33_01520"
     CDS             complement(3767..>5489)
                     /locus_tag="VMZ33_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HUR15939.1"
                     /translation="AHARGIINRDVKPTNVLLTAARRVKVADFGIARAFSEAQLTMPG
                     TTLGSVHYFSPEQARGEVVTPASDVYSAGLVLFEMLTGRRTWTGDSAGAVAVARLTGD
                     PVAPSTIRQGVPPILDHAVRRALSRVPTDRPTAAEMAALLGRYLSDPQRAGTWVGPFS
                     PDGTPVVAGVTAAAGTGAMRAGAVGGAPPQMAGVQTIPSADGYANGGPGSTVGGSLAG
                     GPEYGTIRAGVGPRRYGYQDDEPQEEGAGPWGWIAAILGVLVLFAGGLLLFLLLTSRG
                     APTPTATPASGLVTVPTLVGLELDAAEREAGEFGLFVTVGAFQATDDAPENTILSQDP
                     TAGGGVTPGSRITVIVASRSVTVPVPESRLRTEAELFSVLAQSELLPGNRSESFDSTV
                     PPNLVIGTNPRAGTEVARGTAIDYIVSLGAPPTATPTLLVTPSPEITPSPTIEITVTP
                     TATSTATPTATPTATPTATPTAAPTAAPTATPTVTPTPIVCETPPPSEDPCVTVGNYA
                     QCISLGQSRTQIVEAGLRVGEIFQTAQAEENDDRLVAQQFPEAGTRVPPETPVDLLVT
                     PPAGPCP"
BASE COUNT          903 a         1802 c         1942 g          842 t
ORIGIN      
        1 gcggttgtgc atcacgatca tgggcgcgtt gcgctccgcg gcgaggccgg ccatggcgtc
       61 tgaagcaccc gtgccccaga tgtcgttgat gacgtcagcg ccggcttcga gggccgccgc
      121 cgcaacatcg gctttggtgg agtcgatgct gatcggcacc tcggggagtg ccgcgcggac
      181 ggcgcggacc accgggatga cgcgcgactt ctcgtcggcg gatgagacgt cagcgtgtcc
      241 cggacgtgtt gactcgccgc cgatatcgag gatgtcggcc ccgtcgtcga ccatgcgacg
      301 cgcctgctcc acagcggcct gcaccacggc cgtatcgcgc gacatgatcc cgtcgccgct
      361 gaacgagtcc ggggtcacgt tgatgatccc gagcacgtag gtccgcgcac cccacacgaa
      421 ctcgcggtcg tggatgagga ccgggctggg cggctggtcg gggatgccct gcccgacgtc
      481 cgacggcagc gctccccctg gcacggcgtc catctactcg cctacgagtt gagctcggac
      541 ataaccgaca aggtacagcg aaccgcaaac aaggagcgtc gcgccggcgt cgcgcgcagt
      601 cgccagggca gcttccaacg ccttgtccgt gtcctcgatc cgtctggccc taccggccca
      661 ggctgtatcg atccgcgcga gcgggagcga gttgggcgcg tcgggaacct cggtggcgac
      721 caagagtgcc ccgggtgcgg cggagttcag cgggtcgagc atgccttcct gccagtggtt
      781 ctccaggatt ccgatcagca gcgttatctc gccactcccc agctggggaa gtaactcccc
      841 gagcgcagcg gcgagggcgc gcatcccgtc ggggttgtgg gcgccatcga gcagcacgtc
      901 gaccccacgg acgttgatgt gctcgaggcg gcctggccat tcggccttgg ccagtccggc
      961 agcgcgcacg gccgcggtcg ttgggaaacc cattcggtcg agtccgtcga gcacgccgac
     1021 cgccacggcc gcgttggcct gttggtgagc ccccagcaga ccgagcgcga tgcgctgggc
     1081 cggggccgac gtctcgacca ggggcacatg tactcgccgg gcgcgccccc aaatcaccga
     1141 cagggctggg tgtcccgcac cggtgacggc ccacgcgtcg ccgcgcttga tgatcgctgc
     1201 cttctccctg gcgatcgcct cgaccgtcga cccgaggtat tccatgtggt ccagaccaac
     1261 gttcgtgatc gcgctggccc cgcccgacca gacgttggtg gcgtcgagac gtccgcccat
     1321 gcccacttca accactgcca aatcgacctt ggcccgtcgg aagtaggtga acgctgccgc
     1381 ggtgatcacc tcgaactcgg tcggtgggcc gtggcgtccc tcgacgtgat cagcctgagc
     1441 tagaacctcg tcaatgagat cgcagaagtc gttgatgggg atgtgcgatc cgtcgaccac
     1501 gatccgttcg cggtagctaa cgagatgcgg cttgggcgtc tggccgacgc gatagccgcc
     1561 atgtttcagc acggacgcga gcatggcctg ggtgctgccc ttgccgttcg tgccgccgat
     1621 cagggcgccg ggcaagcccg cctgtggatc acccatccgt cggagaagcg cgcgagtccg
     1681 gccgaggcca agacggatgc caaatcgacc acgctgtctc agccgggcaa gggtgtcctg
     1741 gtagcgcagg ctgacacgct cagccttgag cgaatcagac gttcagctcg tcctcgtaga
     1801 acacgcactg gttgaagcgc ggtgagaggc agatgtcgcc gacgtagctc tgctccaggt
     1861 gcacgccgcc gcggagctgg caacgggcga cgttcatgtc ctggcgcatc agagccttga
     1921 ggaggtgcgg ggccgtgatc gggatcgggc cgcgcggccc cgtcatgtag aaatgcggac
     1981 agacgttgcg acgcaggttc ggcaggccga aggggatcct gggcatcatc gccccggcac
     2041 ccggcggcgg atctgccggt cggcgcatca ggagattgct cgagaccctg ctgatgggtc
     2101 cgcgcggggg ctgtgggggg acgggacggc cgtggagtgc cgggcgcgcg attagcgtag
     2161 ccagcggctt acgcgacgaa tccactcacc ctcccaagac ggcactgcag cgtgcccagt
     2221 cgcagtagcg tacacaagcg ggcactcaaa gtgtgccttc ccggcgcggc cgtggtgagg
     2281 gtgttcgcag gcggccacag ctcgggctga cggtgccaga atcgacccag atgccggcat
     2341 cgtcacgacg cagtgcgggg agcggcggac agagcggtcg ccgcgctggc gcccggccga
     2401 accgccgtcg cacacagaag caatctcaag cggccgtgcg ccgcgtcagc tggccgcgtc
     2461 gcatcggtgc agcaggcgct ttcgtgctca ccgttggggc gagcatggcg ctcgccggga
     2521 acgcgatgcg tgagggacca ggctcgccta cgcagccacc gggcgtacaa accccggggg
     2581 cagcaacgct catccaaccc gctccagaca tcacgcagga cgcgagcgtc gacatcactg
     2641 ggacactgcc gccgaatttg gcgcgcattg gggagtcgac actgcgcgtg ttcgtcaacg
     2701 gcgaccaggt ccgcgagcga gaccttcccg cagagcagca attcgtgctc gatgacgtgc
     2761 cgttggtgga agggcacaat gagatcaccg ttggcctgac ggttgacggc cacagcgggg
     2821 cgcagtcagc gccggtgtcg atcaccagcg acagcactgc gcccgagatc cagatcctgc
     2881 gccccgagcc gggcacgatc gcctacgcag gccgcgagac tatgcgcggt cgaaccgagg
     2941 ccggggcctc gattgagatc tacgcccgcg ggagcgagca ggagctcccg gtctcgatcg
     3001 gcggcgatgg tcgttttgag gcggtgatcg tcctggcgat gggcgacaac gacttcacac
     3061 tgcgcagcat cgacctggcg ggcaatccca ccacggtcca ccacgtgatc acgcgccaag
     3121 tgacgctcgc atccatcacc ctcaactcgt cgatcgaaga ggtggcgctg gccggcctac
     3181 cggcggcggt ggacttgacc gcgaacgtcc gcgatgaaca cggcggcaag ctggagggcg
     3241 tcgatgtggt cttcagcctc tcgccaccga ctcgcgccac gacgacgtac cgcgcaacga
     3301 cgaggaacgg ggtcgcgcgc tggtcgaacg tgtcgattcc tgacagcccc acgtcagtcg
     3361 ggacgtggct ggcgacggtg ctcgtcatcc tgccatcagg cgaggagttg cgcgaggacg
     3421 tcgctttcgt cgtccattga cgaggcactg ctagcgggag gcgggctcca gcttgacgaa
     3481 gatcggacct gccggggctg gcgaggcgct cgaccagcag ccgcgtcgct cttcaccggt
     3541 gagcggcgac ttcctgcggt cgatgccgca gaagccgacg ccgccgatca ccgaacgctg
     3601 gaagttcacg caggtgccgc agctcgtggc tgatcgtccg caaaggaagc acgcggtcga
     3661 gtcggcctga gccgttccgc agtgtggaca ccgccacgtc cccatctgtt tcggagtatg
     3721 cgctttgacg gacgttgtta tcggccgtcg caacacaacc cgaagcctac gggcagggac
     3781 ctgcaggcgg cgtaacgagc aggtcgacgg gcgtctccgg aggcacgcgc gtgccagcct
     3841 cggggaactg ctgcgccacc agccgatcgt cgttctcctc ggcttgcgcg gtctggaaaa
     3901 tttcgccgac gcgcaggccg gcctcgacga tctgcgtccg cgactggccg agcgatatgc
     3961 actgcgcgta gttgccaacg gtgacgcagg ggtcctcgga aggtggcggc gtctcacaga
     4021 cgatcggcgt cggggtgacc gtcggcgtcg cggtcggcgc ggcggtggga gccgccgtcg
     4081 gcgtggcagt cggggtcgcg gtcggggtcg cggtcggggt cgcggtcgac gtcgcggtcg
     4141 gtgtgacagt gatctcgata gtcgggctcg gcgtgatctc aggcgacggt gtgaccagca
     4201 gggtgggagt cgccgtcggc ggagcgccga gcgacacgat gtaatcgatg gcggtcccgc
     4261 gcgcaacctc ggtcccggca cgagggttcg tcccgatcac gaggttcggc ggtacggtcg
     4321 agtcgaaaga ctctgagcgg ttcccgggca ggagctcgct ctgggccagg acagaaaata
     4381 gctcagcctc cgtgcgcagg cgcgactccg gcacagggac ggtcacggac cgcgaggcaa
     4441 cgatgactgt gatgcgcgag ccgggcgtga ctccgccacc ggcggtcggg tcctgcgaca
     4501 ggatggtgtt ttcgggcgcg tcatcggtag cctggaaagc accgaccgtc acgaacagac
     4561 cgaattcacc ggcctcacgt tcggcggcat ccaactccaa cccgacgagc gtcggaacgg
     4621 tgacgaggcc ggaggcaggg gtagccgtag gcgtaggggc gccgcgactt gtcagcagca
     4681 ggaacagaag caggccgccc gcgaacagca ccaggacacc gagaatcgcg gcgatccagc
     4741 cccacgggcc ggcaccttcc tcctgtggct catcgtcctg atagccatag cgccgtgggc
     4801 cgacgccggc acggatggtg ccgtactccg gcccgccggc caacgaacca ccgaccgtgc
     4861 tgcccgggcc gccgtttgcg tagccgtcgg ccgacggaat ggtctgcacg ccggccatct
     4921 gcggtggagc tccgcccacg gcgccggcac gcatcgcacc tgtcccggcg gccgcggtga
     4981 cgcccgcgac tacgggtgtc ccgtcgggac tgaacggccc gacccaggtg ccggcgcgtt
     5041 gcggatcact gaggtagcgg ccgaggaggg cggccatctc agcggcggtt ggccggtcgg
     5101 tggggacgcg ggaaagcgcg cggcggacgg catgatcaag aatgggcgga acaccctggc
     5161 ggattgtcga cggtgccacg gggtcgccag tgagccgcgc tacggcgacc gcgccagctg
     5221 agtcgcccgt ccacgtgcgc cgtccggtga gcatctcgaa caggacgagt ccggctgagt
     5281 acacgtcaga ggcgggcgtt accacctcgc cgcgcgcctg ttccggcgag aagtaatgga
     5341 cagagccaag cgtcgtgccg ggcatcgtca gctgggcctc tgagaaggcg cgcgcgatgc
     5401 caaagtcggc gaccttgacg cgtcgggcgg cggtcagcag aacgttggtg ggctttacgt
     5461 cacggttgat gatgccgcgt gcgtgcgcg
//
LOCUS       DATLCO010000059         3695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3799361, whole genome shotgun sequence.
ACCESSION   DATLCO010000059 DATLCO010000000
VERSION     DATLCO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3799361"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1092)
                     /locus_tag="VMZ33_01525"
     CDS             complement(238..1092)
                     /locus_tag="VMZ33_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HUR15940.1"
                     /translation="MPSEDRRVALPDSAVQYHIDCGPGDLGEYILLPGDQDRTAKVAE
                     LFDSVEFQKRHREFAVATGTYRGRRVSCVSTGIGTDNVEIVVAEILAITKRPTFIRIG
                     SCGVLKDDIALGDLVISSGAVRLEATTKYFVHEGYPAVADYSAVAALVEAAHTLGHRA
                     HVGITATAPGFYGAQGRPIPQLPIRFPDLADEMTRQGVVNFEMEASALLVLAGLAKCR
                     AGVVCTAYAQRVSGDFVQGDAKDRAEKACIETGLEALRLLAEMDEATRAAGAVHWRPS
                     LWTSGMSS"
     gene            complement(1280..>3695)
                     /locus_tag="VMZ33_01530"
     CDS             complement(1280..>3695)
                     /locus_tag="VMZ33_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15941.1"
                     /translation="PAAPLIPTALACEPFEAQQITVEGWVTTAPSVTGLGQITTALDD
                     GSGPLAVIAEPGSGLVSSAFAVGSRIRVIGVLGQLSAGYRLYPRSTADVVVLAPAPTA
                     TPTAAPTATATATPTAAPTVTPTLPPTQTPTASASPTASATASPTPSTTASPSPSPTV
                     ALQAIGDARVRAVGSTVRVRGVITVSPGWILGDSTLAIQDDTGGIYVRVPQASLDLVP
                     GREIEVTGPLADPYGNLEVRPGTAGVTLLGTSSVPAPRPMSAADLAESTEGLLGRVEG
                     TITALDASSTGSLTITVEDETGEARVFAHAPLGVTRDDFDTGQRVVAIGLVGDRLGSY
                     RLWPRSVGDITVVHAVPTPTHSPSPSPTRTPSPTRTASPTATPTRTPTPTRTPTPTVR
                     PSPTPTRVPSLTIAEALRRQGQDVTIEGSVTTPKGLLDADSLRVTVQDQTGAVLVRLP
                     TDFGVGVGQRIRVTGEMGTYYGAPQLSADDAARLGDGAGGALSIAYAPIGAALEWRLV
                     TISGHVLSVHRDGDAWRAELELRGGSVPVVGLERAGIDSSALVEGRPATVTGVVKRAY
                     PTATDQRFAIVPRAAADIRLGAPTTTPSGSEAPGATAGPDGTTGPDWTVGPGGTVPAS
                     SGGSPEPSATVVTSIGDVAGREGSVVVIGGRVIAISGSRLTVDDGSGTAIVELAGGAA
                     SRAGEFMMGDLINVTGLAARDDGGQLLVVVSDPANVTRLSSSAAGAAAPTDATRSVLS
                     DTTGVAAGADPDATQPASATTALVAAALIALALGLLAVVAFASPKHRQRITERVARRL
                     SMLRSH"
BASE COUNT          582 a         1279 c         1234 g          600 t
ORIGIN      
        1 ccctccctcg ttggactcgc gtcagcccgc gccgatccgg aaaacgctgt tcccgcactt
       61 agggcaggtg cccttcatcg cgggcttccc gttcttcatc gtgatcttct cggggttctg
      121 gatctcgacc ttcatcttgt ctctgacgca ataggcttca gccacttgtc ctccttaccg
      181 tcacggtctg ggcgggccaa ggcggggtgc cttggctccg tttttacctc cctctcatca
      241 ggacgacatt cctgacgtcc atagagaggg ccgccagtgc accgctcccg cggcacgcgt
      301 ggcttcatcc atttcggcta gcaggcgcaa tgcctccagc cccgtctcta tacaggcctt
      361 ttcggcccgg tccttcgcgt ccccctgcac gaagtcccca ctcacacgct gagcgtacgc
      421 cgtgcacacg acgcctgctc ggcatttcgc caaccctgcc aatacgagca aagcggacgc
      481 ttccatctcg aaattgacca cgccctggcg cgtcatctcg tcggcgaggt caggaaagcg
      541 gatgggaagc tgcggaatcg gccgtccctg agcgccataa aagcccggcg cggtcgcagt
      601 gatcccgaca tgcgcgcggt ggccgagcgt gtgtgcggcc tcgacgaggg cggccacggc
      661 cgaatagtca gccacggccg ggtaaccctc gtggacgaag tacttggtcg tcgcctcgag
      721 tcggacggca ccgctggaga tcacgaggtc gcccagcgcg atgtcgtcct tgagcacgcc
      781 gcaggaaccg atccggatga aagtggggcg cttggtgatc gcgagaatct ccgcgaccac
      841 gatctcgacg ttgtccgtgc cgatgccagt ggagacgcac gagacacgcc ggccgcggta
      901 cgttcctgtc gcgaccgcaa actcgcggtg gcgcttctgg aactcaactg agtcgaagag
      961 ctcggccacc ttcgcggtcc gatcctggtc ccccggcagc aggatgtact cgcccaggtc
     1021 gcccgggcca cagtcgatgt ggtactgcac cgcggagtcc ggaagcgcga cgcgacggtc
     1081 ctcgctcggc aaaggcactc tccttcagtc ccacgctcaa ctcaaacgtg agcgcggggc
     1141 gcacgttaga gccactctga gcgtggcgtc aacacgcgag gcgcagccag tgggcgctgc
     1201 tgcgccggac tcgcgtagcg gcgcagcacc ggccggcggg ccgggtgccg agttggagag
     1261 cttgaactgg agcgggaact cagtgagatc tcagcatcga cagccttcgc gcgactcgtt
     1321 cggtgatccg ctgacggtgc ttcggagacg caaaggcgac cactgccagc agccccagcg
     1381 ccagcgcaat gagtgccgcc gcgacgagag cagtggttgc agaggccggt tgcgtggcat
     1441 ccggatctgc gcctgccgcg acacccgtgg tgtcgctgag cacgctccgc gtcgcgtcgg
     1501 ttggggccgc ggcgccggcg gccgaagacg acagccgagt gacgttggcc gggtcgctca
     1561 cgacgactag cagctggcca ccgtcgtcgc gcgcggccag gccggtcacg ttgatcaggt
     1621 cgcccatcat gaactcccct gcacgcgagg ctgcgccgcc ggccagctcg acaatcgccg
     1681 taccactgcc gtcgtccacg gtcagcctcg agccactgat cgcgatgacg cgccctccga
     1741 tgacaaccac actcccttcc ctgccggcta catcaccgat cgaggtcacg accgttgccg
     1801 aaggctccgg cgacccgcca cttgacgccg gcaccgttcc gccgggcccc accgtccaat
     1861 cagggcccgt cgtcccgtcc gggcctgcgg tcgctcccgg cgcctcggat ccactcggtg
     1921 tcgtcgtggg tgctccgaga cggatgtcag ccgccgctcg tggcacgatt gcgaaccgct
     1981 ggtcggtcgc cgtgggatag gcccgcttga cgaccccggt gaccgtggcc gggcgacctt
     2041 caaccagcgc cgacgagtcg atgccggccc gctcgagtcc gacaacgggg accgaaccgc
     2101 cgcgcagctc cagctcggct cgccaggcat cgccgtcgcg atggacggac agcacatggc
     2161 ctgaaattgt cacgagccgc cactccagcg cagcaccgat cggtgcgtag gcaattgaca
     2221 acgccccgcc cgcgccgtcg ccgagcctgg cggcgtcatc tgcgctgagc tgtggtgccc
     2281 cgtaataggt gcccatttcg ccggtcacac ggatgcgttg gccgactccc acgccgaaat
     2341 ccgttggcag ccggaccagc acggcgcccg tctgatcctg cacggtgacg cgcagcgagt
     2401 cggcgtcgag caggcccttg ggcgtcgtca ccgagccctc gatggtcacg tcctggccct
     2461 gccgtcgtag tgcctcagca atcgtcagcg agggcacgcg cgtaggtgtt gggctggggc
     2521 gaactgtcgg cgtcggggtt cgcgtcggcg tcggggttcg cgtcggcgtc gctgtcggcg
     2581 aagccgtacg cgtcggggat ggcgtcctgg tcggactggg gctcggtgag tgcgtcggtg
     2641 tgggcactgc atggacaacg gtgatgtcgc cgacacttcg cggccacagt cgatacgacc
     2701 ccagccgatc cccaacgaga ccgatcgcga cgacgcgctg ccccgtgtca aagtcgtcgc
     2761 gtgtcacgcc cagcggagcg tgagcgaaca cgcgcgcctc gcccgtttcg tcctcaactg
     2821 tgatcgtgag gctgccggtg ctgctcgcgt cgagcgcggt gatcgtgccc tccacgcgcc
     2881 cgaggagacc ctccgtcgac tccgcgagat cagccgcgct catcggccgc ggggcgggca
     2941 cggaactggt gccgagcaac gtgacgcccg ctgtgcccgg acgcacctcg agattgccat
     3001 atggatcagc cagcggaccc gtcacctcga tctcccggcc cggaaccaag tccaacgagg
     3061 cctgtggcac gcggacgtag atcccgcccg tgtcgtcttg gatcgcgagc gtcgaatcgc
     3121 cgaggatcca gcctggcgaa acggtgatca caccgcggac gcgcactgtc gacccgacgg
     3181 cgcggacacg tgcgtcgccg atcgcttgca gggcaacggt tggagacggc gacggactgg
     3241 cggtcgtaga tggggtcggc gaggcggtcg cgctcgctgt tggagaagca ctcgcggtcg
     3301 gcgtctgggt cggcggaagc gttggcgtca ccgtgggcgc tgctgtgggc gtcgccgtcg
     3361 ctgtggcggt gggtgctgcc gtgggcgtcg cggttggcgc cggcgcgagc acaactacat
     3421 cagccgtcga gcgcggatac agccgatatc cggccgacag ttgaccgaga accccgatga
     3481 cgcggatgcg cgagccgacg gcgaaggcgg acgacaccaa acccgagccc ggctcggcga
     3541 tgaccgctag cggtcccgag ccatcgtcga gcgcggtcgt gatctggccc agcccggtca
     3601 ctgaaggagc ggtggtgacc cagccctcga cggtgatctg ctgggcctcg aagggctcgc
     3661 acgccaacgc ggtgggaatg agaggcgctg ctggc
//
LOCUS       DATLCO010000060         8712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2308375, whole genome shotgun sequence.
ACCESSION   DATLCO010000060 DATLCO010000000
VERSION     DATLCO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308375"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VMZ33_01535"
     CDS             <1..1005
                     /locus_tag="VMZ33_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15942.1"
                     /translation="VHWYASMLSTTWFLAHVVALTFVVLGITLCLDGERRERMRARFD
                     ALAGAGATHGRRGGAVSLFQESSLFLAWYRRLRQQIDPMQFMAGFVFGIGALARLPVI
                     FGAPFFLFVGGGGTFFRRGLSAGLGAAIPVGLLLLYTFVATGHLFNPAYEHLYQTEYL
                     GYLPPETGSCLFPRSICDSLTINRDLAIEDIGHVPVNLAIMLAWLPVFDADCVTSLLR
                     PECAVLKPDQIGMSVLLTSPAYLLAIPLALANWRKRIVLGSLLAVGAIAFINLMHFSQ
                     GWVQFGYRFSNDFAPFALVLVTLAIASMRKRMGLAIALVAASIIINAWGVYWGVVLGW
                     "
     gene            969..2561
                     /locus_tag="VMZ33_01540"
     CDS             969..2561
                     /locus_tag="VMZ33_01540"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HUR15943.1"
                     /translation="MGRVLGSGPGLVANRIHGGGRLRPALSRAARHTELLAAALVAAP
                     ALVLYGRTLMPDVGFWDTAEFQAIGPVLGIAHPTGYPAFTMLAWVASIVFQPLGNEAF
                     RANLLNAFLAAAAVGVVAAVVTRLTGRLAVGVATGLVFALSAETWAIGLRADPHALHL
                     LLAAVLVYLLVVWHDRVRMAQRADRWLLAAAVTFGIALGNHGLTFLLAPGIAVFVLAA
                     YPQILRRPKLIAACAGGLALTTVLIYLYLPLRSAMNPALDYADPQTWEGFRYLVFAEQ
                     FRGTFRAFPTMAEAFRTIGAETLSQLGLFAILAPAGVLLAAWRRPTLVLLLGLWFLVN
                     WVFALGYLNADIGRYYLVPVLAAAILGGIGLGAIVEGVERVVSRRRMAEGRLLTVATG
                     VILAIICVTPTLAALPGRYRIVDGSRDLAARTWLDAVSHALEPHAVVVSWWSYSTTLW
                     YGQYVEGTRPDISVVDDSTIVQQHLGDVNAVIDSYLGVRPVYLIRLSSDLPNYEQRYT
                     LTSVPGIVGGPVYRVEARLANR"
     gene            2587..3369
                     /locus_tag="VMZ33_01545"
     CDS             2587..3369
                     /locus_tag="VMZ33_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUR15944.1"
                     /translation="MADGTETRGARARVPELSFFFPAHNEAENIREFVAEALAELPTL
                     ADRFEIIAVDDGSRDETPRLADELAAANPGVVRAVHHEVNKGYGAALRTGMASAQYDL
                     VCFLDGDRQFKVADLGRLIDRMARTDEPDVVVGYRIKRADPFIRTAYAKSYRLALNIF
                     YRLGVTDVDCAAKMFRRSSLEGVRLESGGAFLSAELLIKLKQRGRKLTEVGVPHYPRT
                     AGSATGAKPSVIFRAMRDFWRLRIRLWMNRSSALEQGEPVIA"
     gene            complement(3366..3938)
                     /locus_tag="VMZ33_01550"
     CDS             complement(3366..3938)
                     /locus_tag="VMZ33_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HUR15945.1"
                     /translation="MSFGGGERNGERNGHDGGLNEMVVESVRVHMLSSQHVVILRDSA
                     TDRYLPIWIGPWEANAIAMRLQGVTPERPMTHDLFSQTLSELGAEVKQIIVSDLAEDT
                     FRARLLLQIDGREVDLDARPSDAIALAVRAGVPIFATDAVLDRAGVIPESAEEEKLSI
                     FRDFVNSLNVDLPDEGGAAGGGTEERRRLD"
     gene            complement(3948..6245)
                     /locus_tag="VMZ33_01555"
     CDS             complement(3948..6245)
                     /locus_tag="VMZ33_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15946.1"
                     /translation="MSTRLALKYGLVSEDDRLSNSADAIIVTEPTTGSKARTKGSLYL
                     VVTSRAAGGRPRDACRLVADTIRREYYYDESAGISIVLEKSIRAANRRLRHSREGGGL
                     GAGSVGIAAAVVRGNELYVATCGDAESYLIRSARLLMPEHESGEGLPAADNVRVDVWR
                     GDFGVGDSLVLCSRNLVEVVGTEELKNAVVTLHPQSAVEHLHHLFVAAGGDGSDAVLA
                     IEASEVALSRVEHRLVPVSTSDALAGAESKSPIPLADQLSGAAAAVQDRAVGARLAVR
                     DGFSRFVGGILNLTPRRRSTYRRMSTAANRRETQRRAAIAVLAFLGVVAVLGIVVVAF
                     NPLRTETPVSQVNEGEAAFAEAKANADQVFGSADLVTNDPTQAETLLREAWLALGRAE
                     TSGVVSRSSIERERLRVAEGLNTLYGTVTISPTPLYAAQPNGALRDLVVDLLGPDTAA
                     YSIAGTAIVRVDPETGAAATVAQQGDGVGVGLGLPRLIARGGPELVILDDAGLLWRWR
                     PSDTSGGGTLGQINVGGDPDFGGDLADIETFLINPDQGLYRLYVPNPASGQVLKYEPT
                     ADGSGFSVPNPYFVSEGEDVAAFEQLLVDGDVYAITSENVLRYFSGRRSGTFELAVPP
                     DAEDLRPSHDYRMMAATGTRGVGNLYVWDAAHARILAFDKIDGAYVDQFVASPGGVPL
                     TDITGMFVVDRGVEQAPILMFARAEGLYQVVLSATPEEPNASPTGPSASPSEAPAASP
                     TVAPSETPVSPTPEPTQRPRRTPRT"
     gene            complement(6257..6601)
                     /locus_tag="VMZ33_01560"
     CDS             complement(6257..6601)
                     /locus_tag="VMZ33_01560"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HUR15947.1"
                     /translation="MTTRRQPMRVGDVLPGVASTLGIDGELRRARQMAAWQRIVEERV
                     PAASGASGLLDVQPPALVVSATSPIVAQELRLRQYDLLSAFAQVPEGARLIELRVVIR
                     PMGATGRNSAPR"
     gene            complement(6598..7764)
                     /locus_tag="VMZ33_01565"
     CDS             complement(6598..7764)
                     /locus_tag="VMZ33_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HUR15948.1"
                     /translation="MAAKPVTPTEAGMHVGRISLVSFRSYDAIEVSFPRGPQVVVGDN
                     AAGKTNLLESMVALGLGHSHRASAEGELIAWGADFARVEIDLAPEQTRLEMVIARTSA
                     GGGRKRVLVNGVARRPAALAQALPVVLFAPEDMLLIVGSPSGRRAAIDSLIAQTVPAA
                     SATMANYGRALTQRNNLLKSIRDGVAAPEELRFWDHVVINDGSQIVDWRRDAMARLAE
                     PLADAHAEIAPEEPRLQLAYVSKETPGPDETTAGAIKRRLAETAEKEQWNGQTLVGPH
                     RDDVAFVSGGRDLSTFASRGQQRTAILAFKLATTDVLTQTFARPPLLLLDDVFSELDP
                     QRRAHLVRRIGELPQAFVTTTTADDLDPALVAQATVWTVTPGHLERSASGTKGR"
     gene            complement(7803..>8712)
                     /gene="dnaN"
                     /locus_tag="VMZ33_01570"
     CDS             complement(7803..>8712)
                     /gene="dnaN"
                     /locus_tag="VMZ33_01570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HUR15949.1"
                     /translation="ARLFSDLVSGLPSGEKIELEADGNALRIKAGRYQTQLRGIDAEE
                     FPVIPAPGERPTTRVSQKELRRALSQVVFAAATDEARPILTGVLTRMSGDKLTLAAAD
                     NYRIAVRGLPILDPVEDTSLVVPAKSYAELMRVLTDTDDPVEIMLASSKSQILFHVEG
                     VDIVSRLIDGQFPNYQQVLPTTHTTRAVVERDELLKAVRVSALIASSAANVIRMRLGD
                     EGSGTITIAAAADVGETQTDVEAAIEGETMQISFNARYLQEALQSLDQDQLALEFAGP
                     LSPGVLRPPTQDEDYVHVIMPVRTPS"
BASE COUNT         1346 a         2932 c         2922 g         1512 t
ORIGIN      
        1 gttcattggt acgcgtcgat gctgtcgacc acctggttcc tggcacacgt cgtggccctc
       61 accttcgttg tgctgggcat cacgctgtgc ctcgacggcg agcggcgcga gcggatgcgt
      121 gcccgattcg acgcattggc cggggctggt gcgacgcacg gccgccgagg cggcgccgtc
      181 tccctgttcc aggagagctc gcttttcctg gcgtggtatc ggcgcctgcg gcaacaaata
      241 gatcccatgc agttcatggc cggcttcgta ttcggcatcg gcgcgcttgc ccggctgccc
      301 gtcatcttcg gcgcgccgtt cttcctcttc gtaggtggcg gcggaacgtt tttcagacgc
      361 ggcctgtcag ccggtctcgg ggcagcgatt cccgtcgggc tgctgctcct ttacacgttc
      421 gtggcgacgg gccacctctt caacccggcc tacgagcacc tctaccagac cgaatacctg
      481 gggtacctgc caccagaaac agggagctgc ctgtttccgc gcagcatctg cgattcgctg
      541 acgatcaacc gtgacctcgc catcgaggac atcggccacg tcccggtgaa cctggcgatc
      601 atgctggctt ggctgcccgt cttcgatgcc gattgcgtca cctcgctgct gcggccggag
      661 tgcgcggttc tgaagcccga tcagatcggg atgagcgtcc tcctgacgag cccggcttat
      721 ctgctcgcga tcccgctcgc gctggccaac tggcgcaagc gaatcgtgct cggctctttg
      781 ctggcagtcg gagcgatcgc cttcatcaac ctgatgcact tcagtcaggg ctgggtccag
      841 ttcggatacc gcttcagcaa cgactttgcg ccatttgccc tggtcctggt gactcttgcc
      901 atcgcttcca tgcgcaaacg gatggggctc gcaatagcac tcgtcgccgc ttcgatcatc
      961 atcaacgcat ggggcgtgta ttggggagtg gtcctgggct ggtagccaac cgcatccacg
     1021 gcggcgggcg gctgcgccct gcgttgtcgc gcgccgcgcg tcatacggag ctccttgccg
     1081 cagcactggt tgctgctccg gcgctcgtcc tctacgggcg cacgctcatg cccgacgtgg
     1141 gcttctggga cactgccgag ttccaggcca tcggaccggt cctgggcatc gcccatccga
     1201 cgggctaccc ggcattcacg atgctcgcct gggttgcctc gatcgttttc caaccactcg
     1261 gtaacgaggc gttccgcgcc aacctgctga acgccttcct cgcggctgcc gcggtagggg
     1321 tcgtcgccgc ggtggttact cgactgaccg gccgactggc cgtcggcgtg gcgacgggcc
     1381 tcgtgttcgc gctcagcgcc gaaacgtggg ccataggcct gcgcgcggat ccccacgcac
     1441 ttcacctgtt gctggcggcc gtgctcgtgt acctcctggt ggtgtggcac gaccgcgtcc
     1501 gcatggcaca acgagcggac cgctggttgt tggcagcggc tgtgacgttc ggcatcgcgc
     1561 tcggcaacca cggactgacg ttcctgctcg cgccgggtat cgccgttttc gtgcttgctg
     1621 cataccccca gatcctgcgg cggcccaagc tgatcgccgc ctgtgcgggc ggccttgctc
     1681 tgaccactgt cctcatctac ctgtacctgc cgctgcgctc agccatgaac ccggcgctcg
     1741 actacgccga tccacaaacg tgggaggggt tccgttacct cgtctttgcc gagcagtttc
     1801 gaggcacctt cagggcattc cccacgatgg ccgaggcctt ccggacgatc ggcgctgaga
     1861 cgctctcgca gctgggactg ttcgcgatcc tggcgccggc cggcgttctc ctggcggcct
     1921 ggcgccgccc gacgctggtg ctcctcctgg gcctctggtt cctcgtgaac tgggtgtttg
     1981 cgctcggcta cctcaacgcc gacatcggcc gttattacct cgtcccggtt ctcgcagcgg
     2041 ccatcctcgg tgggatcggg ctgggcgcca tcgtagaggg cgtggagcgt gtcgtctcgc
     2101 gcaggcgcat ggcggaggga cgcctcctca cggtcgccac gggagtgatc ctggcgatca
     2161 tctgcgtgac tcccaccctc gccgcgctcc cgggccgcta ccgcatcgtc gatggctcac
     2221 gcgacttggc cgcccggacg tggctcgacg cggtgagcca cgcgctcgag ccccatgcgg
     2281 tcgtcgtgag ctggtggagc tactcgacca cgctctggta cggccagtac gtcgaaggca
     2341 caagaccgga cataagcgtc gtagatgact ccacgatcgt tcagcagcat ctgggcgatg
     2401 tcaacgccgt catcgactcc tacctcgggg tacgtcccgt atacctcatc cgcctatcgt
     2461 ccgacctgcc gaactacgag cagcgctaca cgctgacgtc cgtgccgggc atcgtcggtg
     2521 gaccggtgta cagagtggag gctcggcttg cgaaccgata accccgccgt cgcagataat
     2581 ccggtcgtgg ctgatggcac cgaaacgcgc ggcgcgcgcg cgcgcgtccc ggagctttcc
     2641 ttcttcttcc cggctcacaa cgaggcggag aacatccgcg agttcgtcgc ggaggcgctc
     2701 gcggagctgc ccacgctggc cgataggttc gagataatcg ccgtcgacga tggcagccgc
     2761 gacgagacgc cgcgactggc cgatgagctg gccgcagcca acccaggcgt cgtccgggcc
     2821 gtgcatcacg aagtcaacaa gggctacggc gctgccctgc ggacgggcat ggccagcgct
     2881 caatacgacc tcgtctgctt cctcgatggc gatcgacagt tcaaggtggc cgaccttggc
     2941 cggctaattg atcgcatggc tcggaccgat gagcccgacg tggtggtggg ctaccgcatc
     3001 aagcgcgccg accccttcat ccgcacggcg tatgccaaaa gctatcggct ggcgctgaac
     3061 atcttctacc gactcggagt gacggatgtc gactgcgccg caaaaatgtt tcggcgcagc
     3121 tcactcgaag gcgtgcggct tgagtcgggt ggtgcgttcc tgtcggcaga gcttctgatc
     3181 aagctgaagc agcgcgggcg caagctgacc gaggttggcg tgccccacta cccccggacg
     3241 gccggttcgg cgacaggtgc aaagccatcg gtgatcttcc gcgcgatgcg cgatttctgg
     3301 cggctgcgaa tccgcctctg gatgaaccgc tccagcgcgc tcgagcaggg tgagcccgtc
     3361 atcgcctagt cgagtcggcg ccgctcctcc gtcccaccgc cggccgcgcc gccctcatcg
     3421 ggaaggtcca cgttcaagga attcacgaag tcgcggaaaa tcgagagctt ctcctcctcc
     3481 gcactttccg ggattacgcc ggcacgatcg agcacggcgt ccgtagcgaa gatcggcacg
     3541 ccagcacgca ctgcaagggc gatcgcatct gagggccgcg cgtcgaggtc cacctcgcgg
     3601 ccgtcgatct gtagcagcag ccgcgcgcgg aaggtgtcct cggccaggtc ggacacgatg
     3661 atctgcttga cctcggcccc gagctcgctg agggtctggc tgaacaggtc atgagtcatc
     3721 ggccgctccg gcgtaacgcc ctggaggcgc atggcaatcg cgttcgcttc ccagggtccg
     3781 atccaaatcg gcaggtagcg gtcggtcgcg gagtcgcgca ggatcacgac gtgctgggac
     3841 gagagcatgt ggactcggac gctttcgacg accatctcgt tgaggccgcc gtcatggccg
     3901 ttccgttcgc cgttccgttc gccgcccccg aaggacatgt gccgattcta cgttcggggc
     3961 gttcgccgtg ggcgctgggt cggctcaggc gtcggcgaca ctggcgtctc gctcggcgcg
     4021 acggtcggcg aggccgccgg tgcctcgctt ggcgaggctg aggggccggt cggtgacgcg
     4081 ttcggttcct caggtgtggc cgataggacg acctggtaca ggccctcggc gcgggcaaac
     4141 atgaggatcg gcgcctgctc gacgccgcgg tcgacaacga acatgcccgt gatgtccgtc
     4201 agtgggacac cgcccggcga cgcgacgaac tgatcgacgt aagcgccgtc gatcttgtcg
     4261 aaagcaagaa tgcgtgcgtg cgcggcgtcc cagacgtaca ggtttccaac gccgcgggta
     4321 ccggtggcgg ccatcatccg atagtcgtga ctggggcgca ggtcttccgc gtcgggcggg
     4381 accgccagct caaacgtgcc cgagcgacga ccgctgaagt agcgcagcac gttctcggag
     4441 gtgatggcgt agacatcgcc gtcgacgagc agctgctcga acgcagccac gtcctcgccc
     4501 tcgctcacga agtagggatt cggcacagag aagccactgc cgtcggcagt cggctcgtac
     4561 ttgagcacct ggccgctcgc tgggttggga acgtacaggc ggtacagacc ctggtcgggg
     4621 ttgatcagga atgtctctat atcggccagg tcgccgccga agtccgggtc gccgccgaca
     4681 ttgatctggc cgagagtccc gccacccgac gtgtctgagg ggcgccagcg ccagagcagg
     4741 ccagcgtcgt cgaggatgac cagctctggc ccgccgcgcg cgatgagtcg cgggagcccg
     4801 aggccaacac cgacgccgtc accctgctga gcgacagtcg ctgccgcgcc ggtctccgga
     4861 tcgacacgta cgatggccgt gccggcgatg gagtacgccg cggtgtccgg gccgagaagg
     4921 tcgaccacca agtcgcgcag ggcgccgttg ggttgggccg catagagcgg cgtcggcgat
     4981 atcgtgaccg tgccgtagag cgtgttcagg ccttccgcca cgcgcagccg ctcgcgctcg
     5041 atcgacgacc ggctgaccac gcccgaggtt tccgcgcgac ccaaagctag ccacgcctcg
     5101 cggagcaggg tctcggcttg tgtcgggtca ttagtaacta ggtcagccga accgaacacc
     5161 tggtcggcat tggccttggc ttcggcgaag gcggcctcac cctcgttgac ctgggagacg
     5221 ggcgtctccg ttctcaacgg attgaatgcg acgacgacga tgcccagcac ggcgaccacg
     5281 ccgaggaatg ccagtacggc aattgcggcg cgtcgctggg tctcgcgccg gttggcggcc
     5341 gtgctcatgc gccggtaagt ggagcggcgg cgtggggtca ggttgaggat cccaccgacg
     5401 aatcgcgaga acccgtcacg gacagcaagc cgggcgccga cagcgcggtc ctgcaccgcc
     5461 gcggccgcgc cggacagctg atcggccagc ggaatgggcg atttggactc ggcacccgcg
     5521 agcgcatcgc tcgtgctcac cggtaccagg cggtgctcga cacgtgagag tgcgacctcg
     5581 ctggcctcga tcgccaggac cgcgtccgag ccgtcaccgc cggcggccac gaacaagtgg
     5641 tgcaggtgct ccactgccga ttgcgggtga agcgttacga cggcgttctt gagctcctct
     5701 gtcccgacga cctcgacgag gtttcgggag cacaggacga gcgagtcgcc gacgccgaag
     5761 tcgccgcgcc acacgtcgac acgaacgtta tcggcggcgg gcaggccctc gcccgactcg
     5821 tgttcaggca tgagcagtcg cgcggaccgg atgaggtagc tctcggcgtc gccgcacgtc
     5881 gccacgtaca gctcgttgcc gcgtacgacg gcggcggcaa tccccaccga tccggcgccg
     5941 aggccgccgc cttcgcggga gtgtctcaag cgccggttgg cagcccggat cgacttctcc
     6001 agaacgatcg agatcccggc ggactcatcg tagtaatact cgcgccggat cgtgtctgcg
     6061 acgaggcggc acgcgtcgcg cgggcgccca cctgccgcac gcgaggtgac gacgaggtag
     6121 aggctgccct tggtgcgggc cttggagccg gtcgtcggct cggtgacgat gattgcatcg
     6181 gccgagttcg agagccggtc gtcctcggag accagcccat acttgagcgc gagccgagtc
     6241 gacatcggcg gtgagtctag cggggagcgc tgttgcggcc ggtcgcgccc atcggccgga
     6301 tcacgacacg cagctcgatg agtcgcgcgc cctctggcac ttgcgcgaat gcggaaagca
     6361 ggtcgtattg ccgcagacgc agctcctggg ccacgatcgg cgacgtggcg ctcacaacga
     6421 gcgccggcgg ctgaacgtca agtagccccg acgcaccgct ggccgctggg acgcgttcct
     6481 ccacgatccg ctgccacgcg gccatctgac gagcgcgccg cagctcgcca tcaatcccga
     6541 gcgtggaagc cactccaggc agcacgtcgc ccacgcgcat gggctggcgc cgggtggtca
     6601 tcggcctttg gttcccgacg ccgaacgctc cagatgaccc ggcgtgaccg tccacaccgt
     6661 agcttgggcg accagcgcgg gatcgaggtc gtcggcagtg gtagtggtca caaacgcttg
     6721 gggcaactca ccgatgcgac gaacgagatg ggcgcggcgc tgcggatcca gctcggaaaa
     6781 tacgtcgtcc agcaacagca gcggcggcct ggcgaacgtc tgggtcagga cgtcggtcgt
     6841 ggccagcttg aacgccagga ttgcggtccg ttgctggccg cgcgaggcga acgtggagag
     6901 gtcccggccg ccgctgacga acgcaacgtc gtcacggtgc ggcccgacga gcgtctgtcc
     6961 gttccactgc tccttctcgg ccgtctccgc gagccggcgc ttgatggcgc ccgcggtcgt
     7021 ctcgtcggga cccggcgtct ccttcgacac gtaggccagt tggagccgtg gctcctccgg
     7081 ggcgatctcg gcgtgcgcgt cggccagcgg ctcggccagg cgggccatgg cgtcgcggcg
     7141 ccagtcgacg atctgggacc cgtcgttgat gactacgtgg tcccagaagc gcagctcctc
     7201 gggggccgca acgccatccc ggatcgactt caggaggttg ttgcgctggg tcagggcgcg
     7261 accgtaattt gccatcgtcg ccgacgcggc gggcacggtc tgcgctatga gcgagtcgat
     7321 cgcggcacgc cgaccgctgg gcgacccgac gatgagcaac atgtcctcag gcgcgaacag
     7381 gacgacgggg agcgcctgcg cgagggctgc cgggcgccgc gccacgccgt tgacaagcac
     7441 gcgcttgcgg ccaccgccgg ccgacgtccg cgcaatgacc atctcgaggc gggtctgctc
     7501 gggtgcaagg tcgatctcga cccgcgcaaa gtcggcgccc caggcaatga gctcgccctc
     7561 ggccgaggcc cgatgggagt gccccaggcc aagggcaacc atgctctcga gcaggttcgt
     7621 cttccccgcg gcgttgtcgc cgacgacgac ctgtggaccc cgcggaaagc tgacctcgat
     7681 ggcgtcgtac gagcgaaatg acaccaggct gatccggccg acgtgcatgc ccgcctccgt
     7741 gggagtgacg ggcttggcgg ccattggcgg tgcggcctcg ggtgccgcct cgaacatggc
     7801 ggtcaggacg gcgttcgcac cggcatgatg acgtggacgt agtcctcgtc ctgcgtcggc
     7861 gggcgcaaca caccgggtga cagtggcccg gcgaactcca gcgccagctg gtcctgatcg
     7921 aggctctgga gagcctcctg gaggtagcgc gcgttgaacg agatctgcat cgtctcaccc
     7981 tcaatcgcgg cctcgacgtc tgtctgggtc tcaccgacgt ccgcagccgc ggcaatggtg
     8041 atcgtgcccg agccctcgtc gccgaggcgc atccggatga cgtttgcggc cgagcttgcg
     8101 atcagcgccg agacacgcac cgccttgagc agctcgtcac gttcgacgac ggcacgcgtc
     8161 gtatgcgtcg tcggcagcac ctgctggtag ttcgggaact ggccatcgat gagacgactg
     8221 acgatgtcca cgccttcgac atggaagaga atctggctct tggagctcgc gagcatgatc
     8281 tcgaccgggt cgtcggtgtc agtcaggacg cgcatcaact cggcgtacga cttggccggc
     8341 acgaccaggc tcgtgtcctc gaccgggtcc aggatcggca ggccgcgcac ggcgatccgg
     8401 tagttgtccg cggcggccag ggtcagcttg tcgcccgaca tgcgcgtgag aacgccggtc
     8461 aggatcggac gtgcctcgtc ggtggccgcg gcaaagacga cctgcgacag cgctcggcgg
     8521 agctccttct ggctgacccg ggttgtcggc cgctcgcctg gggcggggat caccggaaac
     8581 tcctcggcgt cgatgccgcg caactgcgtc tggtagcgcc cggccttgat gcgcaacgcg
     8641 ttgccatccg cctcgagctc gatcttctcg cccgacggca acccactgac gagatcggag
     8701 aagagccgcg cc
//
LOCUS       DATLCO010000061        15706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1564436, whole genome shotgun sequence.
ACCESSION   DATLCO010000061 DATLCO010000000
VERSION     DATLCO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15706
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564436"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VMZ33_01575"
     CDS             complement(<1..723)
                     /locus_tag="VMZ33_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUR15950.1"
                     /translation="MTGATQTGAEPMAAGVPTFGNYIGGEWRPAESGLTFESRNPADT
                     RDLIGNFAASDASDVAAGIRAAEDALPAWRGMPAPKRGEILFNLGALMTQHKERLARE
                     MTREMGKVLAEARGDVQEGIDICYLMAGEGRRMFGDTTPSELPSKWAMSIRQPIGIAG
                     IITPWNFPIAIPCWKMSAALICGNTVVLKPASDTPHCAALLVELMAEAGFPPGVVNLV
                     TGSGEEVGMPLVENPDVNVISFT"
     gene            complement(841..1311)
                     /locus_tag="VMZ33_01580"
     CDS             complement(841..1311)
                     /locus_tag="VMZ33_01580"
                     /inference="COORDINATES: protein motif:HMM:NF022167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15951.1"
                     /translation="MDAREALAIVADLTRAGVRHVVSGGWGIDALVGRQTRVHGDLDI
                     GVDAVHLAEVIALLARRGYSIALDQSPARLALHAPGGHAVDLHPISWDGTGMGRQQGF
                     DGEVYDYPPGEIVEGRIGGGRVRCISAALQRRFHSGGPQRERDTADLGQLDASE"
     gene            1441..2532
                     /locus_tag="VMZ33_01585"
     CDS             1441..2532
                     /locus_tag="VMZ33_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUR15952.1"
                     /translation="MRVGVVGAGLIAQVMHLHYLRELVDRYEVVALCDIVPGNAAAVA
                     ARFGIDGVFSDWREMLAEPLDAVLVLTSGDHAPLAIGAARRGIHAFVEKPMCYSTTEG
                     LTMVQAARDAGTTLMVGYQKRYDPAYLRFRDELAAVEDARLLQVTTLESPYWPYVDHY
                     STIPPRPPSAEQREAMRAVADAAIRQGIGDAEALARHVYEGVLLASLVHEINALRGLL
                     GEPDVLEHASLRPGSLSLLMRFGALPVTLDRVDLPGMTRYRMEFALHGPTRRTTLSFP
                     SPFLRNEPTTIRIEEGAPGTTRSSVREEITSYESGFKRELIAFHEAITTGAPMETPGS
                     DAVRDIALCQAIIAAHATGAPVERPTEMR"
     gene            2529..3452
                     /locus_tag="VMZ33_01590"
     CDS             2529..3452
                     /locus_tag="VMZ33_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HUR15953.1"
                     /translation="MTVRLANAPVSYGVFELTLDSDRFLAPAERVLDEVRSAGYAGID
                     LGSVGYLGDAAGLGERLADRGLGLAGAYIELRFEDRIALDAGRPYLESALDMFDAAIR
                     ADAGQHPAPRPTLAAASSPSHQRRPGGAQHDRSIGLDDSEWARFVDGLQSVAARCRER
                     GYEPAFHHHAGTAIEAPWEIERLLDTSDVGLCLDTGHLTVGGGDPVADLLRWGPRIRQ
                     LHLKDARRADLDQIAADGGSATEIWTRGVFCPLGSGDLNVAGVVDAIVTIGYDGWIVV
                     EEDVLIADEAGFERAAVHQRANRVLLQRLGL"
     gene            complement(3429..5075)
                     /locus_tag="VMZ33_01595"
     CDS             complement(3429..5075)
                     /locus_tag="VMZ33_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HUR15954.1"
                     /translation="MQRPPLWWQSGATYQIYPRSFADASGDGVGDLPGIIGRLEHLAW
                     LGVDAIWLSPFYRSPMADFGYDVADYTDVDPLFGTLADFDRLIAETQRRGIRVIVDYV
                     PNHTSDQHRWFLDSRSSLISPKRDWYTWRDPGPDGGPPNNWVSMFGGSAWERDPTTGQ
                     YYLHSFLKEQPDLDWRNPEVHAAMLDVLRFWLDRGVAGFRIDVANFVAKDPLLRDNPP
                     HPAPGEVAHLSEWNRQVHLYDHAHPDFHEIYRDVRSLVDSYPGDRVTIGELHHPDLDV
                     WAGFYGENLDEIHLPFNFHLLHASWDASSVRNVVNGVVAALPRSAWASWVLGNHDQAR
                     VASRVGIAQAPVAMMLLLTLPGAPTIYYGDEIGMENGIISPEAVRDTWGITEPGYSRD
                     PERTPMHWDGSPNAGFCRAGVAPWLPLAPGWEQRNVAAQRADDGSLLNLTRRLLALRR
                     ENPALAHGDYLPLDGTPPDVFAYLRRADGAPDVLITLNFADAKRTMRCENASRVLAAT
                     DHQRRAGERLGETFVLGPNEGLVIELTGARWPGSEAQPLK"
     gene            complement(5075..6931)
                     /gene="iolD"
                     /locus_tag="VMZ33_01600"
     CDS             complement(5075..6931)
                     /gene="iolD"
                     /locus_tag="VMZ33_01600"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HUR15955.1"
                     /translation="MTGSSLRLTAAQALIAYLGGQNVERDGRVTPFFGPVLGIFGHGN
                     VGGIGEALEGQHDRVRFMPFRNEQAMVHAAAGYAWMHRRLGALACTTSVGPGATNMVT
                     GAAGATINRLPVLLLPGDIFAGRSARPVLQELESRAGRDVSVNDTLRPVSRFWTRIMR
                     PEQLLDALPEALRVLTSPAETGAVTIALPQDTQTEAYAWPESFLRPRTWHVPRGEPEP
                     QLADHVARLIAAAERPMIVAGGGVRYSAAENDLAEFAEEFALPVALTQAGRSTLAHEH
                     PMNLGAIGATGGLAANRYAADADLVIALGTRLGDFTTASGTTWSTPGVRFVAINVDAA
                     DAHKVGAIAVVADARQALRALSSRLRSMDGPGTPRAVERTTRVNALRSEWDALATSLT
                     APGTTGSGRPTQAQLIAAVNEFSGSSGTIISAAGSMPGDLHKLWRSATPDDYHVEYGY
                     SCMGYEIAGGLGVKLAEPAREVVVLVGDGSYLMMNSEIVTAVEHGLRLTVVVVDSHGY
                     RSISALASSLGARNDFNLLATPVDYRAHAQSMGAHAVRAETLAELRAALDGAREFART
                     TVVVVEVDPANEVPSYGWWDVPVAEVSSSAAVVRARAAYERARAASGLVEER"
     gene            6958..7839
                     /gene="iolB"
                     /locus_tag="VMZ33_01605"
     CDS             6958..7839
                     /gene="iolB"
                     /locus_tag="VMZ33_01605"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HUR15956.1"
                     /translation="MPQAVIHGAAVPSTLSADELHLRSHAGGIEVDPPRVGWRYLSFR
                     ALHLDGSPYSLDPALEHAVVVIAGAGVTVVDASGDGIEVAGRPSPFDALPSACYVPAG
                     EPSELVGSSSLVAIASSPTSGRSGVARARVAIRPSDVRIEIRGAGNATRQINHIISPD
                     FPADRLEVVEVLTPSGNWSSWPPHKHDVDDMPAEAVLEEVYYYQFRRREAWGIQRLYR
                     RDRSRDHAWEVRAGEAVLVTDGYHPFVASHGDDAYYLNALAGDRRTMACSFDPDYDWV
                     RGTWGAVEPDTRVPLVR"
     gene            7845..10586
                     /locus_tag="VMZ33_01610"
     CDS             7845..10586
                     /locus_tag="VMZ33_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HUR15957.1"
                     /translation="MPDTSEVAITTIHFVPHTHWDREWYQPFQVFRMRLIALVDDVLN
                     HLEREPAFRFTLDGQLATIDDYLEVRPQAEERIRHFVAEGRLAIGPWQILMDEFLVSG
                     ETIVRNLQRGWQRALAFGAPMSIGYLPDMFGHIAQMPQILRRAGIDRAVVWRGVPAQV
                     TRNVFRWRAPDGSDVVTEYLVGGYGNAAYLLEVPGAAADKVTGYRKAFGPWYGDESLL
                     AMYGTDHMSPVPEMVALLEGVNAADSGVKVVIETLADYMAGRGDGDGDGGHLLVEGEL
                     RSAAHSNMLMGVNSAHIDIKAAAARAERGLSRYAEPLAALHGDEWPERLLELGWQRVI
                     DSSAHDSICGCSVDPVAAQVLVRLDEAAQIAGEIAAQTARRVAMSVPHGDWLWLNPTP
                     AARSGLVELDVLAADDTPEVALQLADGRLLATQEVDRNSPLMLETELRASEVGTFLRR
                     RMHGRELFGKQLNAATVTSGTVTLVLDSEADPEWLDVDQLRTEIEAAVTAAPEENWRV
                     RVISAGRRRVRAMVPAPPLGWSSGRPVGGSGTLEEPVRVSGRSLDNGLVSVSVEADGT
                     LTIVRDSITVSGIGRLVDGGDAGDSYNYAPPFEDRMIESSGTQPTLIMASGPVVGELL
                     IRRAYALPVGLNADHRSRSARTVETEIDMHVVLRAGEPFVRIRLAFDNQADDHRLRLH
                     LPLVEPTDHSSSEGQYAVVRRGLTCEAGHGEVPLPTFPAYGWVDAGGVGILLDHVTEY
                     ELIDGSQLALTCLRSIGMISRNDNPYREDPAGPERPMPAAQLRGRREFSIGVLVHGKA
                     WHAADVIGAAEQYAHDFVVASGTKRRDRTPAESSGGLSVSGSGVVTTALRRVGDWLEL
                     RVVAESPTATEATIGDGLTAAREVDLLGRIVAGLAIADGSLRVPLEPWQIRTIQLHR"
     gene            complement(10587..11426)
                     /locus_tag="VMZ33_01615"
     CDS             complement(10587..11426)
                     /locus_tag="VMZ33_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HUR15958.1"
                     /translation="MAGGAAGGVSRSRSPLTVVSYNVRAGIGPGPFPPAWWRRIDDSR
                     LRRMSDFIALAGADVVALQEVGLASVEALAVDQPAFFADLTGLEVRYAAVSAYPLVEP
                     DSRRVVGATLWGNVILSRHPIEAATTHALPVATDDDPTDPVDTEPRCALACEIATPDG
                     RLTFMSTHLAYLGSRARRLQADRLAEIVDATGGPLVLAGDLNAAVERPELRALTTILD
                     DGFASVGIPVGDARRESCGVDRIDHVLARGFQFTECQVAREAGDLSDHWPVIARLRFA
                     DPA"
     gene            complement(11434..12330)
                     /locus_tag="VMZ33_01620"
     CDS             complement(11434..12330)
                     /locus_tag="VMZ33_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HUR15959.1"
                     /translation="MTGDAAMAGTDEHHRAETLSGRVLRLLNRAPIHIALALVGLVWL
                     APTVGLLVTSFRPRGDIQETGWWESIFTLRFTTENYGFVLEAEGLTQAFINTLLIAVP
                     STLLPIVLCSMAAYAFSWLRFPFRDTLFLLVVALLLVPVQLAFIPLLLMFRPTGLLTG
                     YAAVWLTHTAFALPFGIFLLRNFFITLPRDLIEAARIDGASTIGIFRTIVVPLSVPAI
                     AAYGIFQFLWVWNDLLMALVFIQDGRLHPMTVAVNSLLGTYGSEWHLLAAAAFLLMTV
                     PLLVFFALQRYFVQGLLAGSVK"
     gene            complement(12327..13274)
                     /locus_tag="VMZ33_01625"
     CDS             complement(12327..13274)
                     /locus_tag="VMZ33_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HUR15960.1"
                     /translation="MATAAPVGVVVKPKPVRPSGRRFDLSAIFFIGLPAAFLVVFIII
                     PTSYTFLLSFNRGRRGEFTEWVGLDNYVSLLNDRSFINLSTFPPSGAIWNNMLWILLY
                     VSIVIVLGLIIAVLASRVRYESVIKAIVFLPMAIAATALAVIWRFVYAPDTQTGLLNA
                     ILDPVTAGPVSFLGDPDLVNFALIAAGIWGSVGFATVILSAALKAIPTEILEAARVDG
                     ATERHIFFRIIVPMVSLPMSVLAVTLIVNVIKLFDLIFVMTRGGPGVSSRVIAFTMYQ
                     EAIPGGLYGKGAAVAVIMMLILIPIMFYNVRRFRSEAVT"
     gene            complement(13360..14610)
                     /locus_tag="VMZ33_01630"
     CDS             complement(13360..14610)
                     /locus_tag="VMZ33_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR15961.1"
                     /translation="MSQAPESQAPESQAPESQAPSEGALRVLSLWGGSEREAFLNVLD
                     AFTAETGIPYNFETQRTDYAGVLQSRISGGNAPDIAIIPGIGFLRRFAADQSLVPVSE
                     LGVDIASLGELYPQGFVDSGTVNGEVYGVPAKYNSKGTIWYRPDLFAAANVEVPTTWE
                     EFKAALDALRDTPGALGLGAADDWTLTDWFESIYLRQAGPEAYDTLFSADGDWSDPTV
                     QTAVDTMLEVLNENYVTGGVDAALGRGFVDGIAQAFSETPEAAIYYEGGFVGGIARGD
                     VNPNLVIGETIDWFPFPSINNTADQTTFGGDVVGAFSNSAAAQAFMQFIITPEANTAW
                     ASTGAIVSPHKGVGTDAYPNELVVKEAEQLANAEAIRFDGSDLLPAGVAGESLGALLQ
                     RALRGETIDWADYQSRVQAGWEAE"
     gene            complement(15019..>15706)
                     /locus_tag="VMZ33_01635"
     CDS             complement(15019..>15706)
                     /locus_tag="VMZ33_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR15962.1"
                     /translation="LLRSSHIWLLAPTWIAVNAALGVYAGTGLFNLVRDQSGRYPDQL
                     LAGGLSPVEISVGLAAVGLVFVAGVIYWGNRFKNMRRTTIIFYGIVGGFALVAAAIAF
                     NHSGGIEPILRLPLAVIAGAGLFVLAGATPAALGLLADMSEAYPDDRGAVMGLYGVFL
                     AVGQIVGTMAGGIASDAAGIDGLLMATLIFMAIALPPLFWLRRDEHVFKLEGDPVTAV
                     ERDPPPGQQP"
BASE COUNT         2649 a         5211 c         5349 g         2497 t
ORIGIN      
        1 cgtgaacgag atcacgttca cgtcggggtt ctcgacgagc ggcatgccga cttcttcgcc
       61 cgagccggtc acgaggttga cgacgcctgg cgggaagccg gcctcagcca tgagctcgac
      121 aagcagcgcc gcgcagtgcg gggtgtcgct cgctggcttg aggacaacgg tgttgccgca
      181 gatcagcgcc gcgctcatct tccagcaggg aatggcgatc gggaagttcc agggcgtgat
      241 gatccccgca atgccgatcg gctggcggat gctcatcgcc catttgctgg ggagctcgga
      301 cggcgtggtg tcgccgaaca tgcgccgccc ctccccggcc atgaggtagc agatgtcgat
      361 gccttcctga acatcgccgc gggcctcggc caggaccttg cccatctcgc gggtcatctc
      421 gcgggcgaga cgctccttgt gctgggtcat gagcgcgccg aggttgaaca ggatctcgcc
      481 gcgcttcgga gcgggcatcc cacgccacgc gggcagtgcg tcttcggcgg cgcgaatccc
      541 ggccgcgacg tcactagcgt cgctcgccgc gaaatttccg atcaggtcgc gcgtgtctgc
      601 agggtttcgg ctctcgaagg tgagaccgct ctcagcaggg cgccactccc cgccgatgta
      661 gttgccgaac gtcggcacgc cggctgccat cggctccgct cccgtctgag tcgcacctgt
      721 catggcggac atcgtagtca ccacgtgacg gagtacagtc cgtcaccggt cgcaactccg
      781 cgcgaggcgc gccagatcgg cgccagcggt agtcccagcg gcgcaccgtc agccgccgta
      841 tcactcggac gcgtcgagct ggccaagatc ggccgtgtcc ctctctcgtt gtggaccgcc
      901 ggaatggaac cgccgctgga gagcggcgct gatgcagcgg acgcgaccgc cgccgatgcg
      961 gccctcgacg atctctccgg gcgggtagtc gtacacctcg ccgtcgaagc cctgttggcg
     1021 gcccatgcct gtcccatccc aggagatcgg atggaggtca accgcgtgcc cgccgggcgc
     1081 gtgcagcgcg agccgcgcgg ggctctgatc gagggcgatc gagtagccgc ggcgggctag
     1141 aagggcgatg acctcggcga ggtggaccgc gtcgacgcca atatcgagat cgccatggac
     1201 acgcgtctgg cggccgacca acgcgtcgat cccccaacca ccggagacga cgtgcctgac
     1261 gccagcccgt gtgagatcgg cgacgatggc cagagcttcg cgtgcgtcca tgaccatcat
     1321 cggattttga acggcaggcg atgctaacgt gtatgtgatg gtcgccggca cgtcacatcc
     1381 aggccgcggc tgtaccgatc aggtttgacg atcgaaacgt ccgcgcgcgt ccggggcaga
     1441 gtgagggtcg gcgtcgtcgg ggccggcctg atcgcccagg tgatgcacct gcactacctg
     1501 cgcgagctcg tcgacaggta cgaggtcgtg gcgctgtgcg acatcgtccc cgggaacgcc
     1561 gccgcggtcg cggctcggtt cggtatcgat ggcgtcttca gcgactggcg cgagatgctt
     1621 gccgagccgc tcgacgccgt cctcgtgttg acctcaggcg atcacgcgcc gcttgcgatc
     1681 ggggccgcgc gccggggtat ccatgcgttt gtcgagaagc cgatgtgcta ctccacgacg
     1741 gagggcctga ccatggtcca ggccgcgcgg gatgccggca cgacgctgat ggtcgggtac
     1801 cagaagcgtt acgaccctgc ctacctccgc ttccgcgacg agcttgccgc cgtcgaggat
     1861 gcccggctgc tccaggtcac tacgctcgag tcgccctact ggccatacgt cgaccattac
     1921 tcgaccattc cgccgcggcc tccctcagcg gagcaacgcg aggccatgcg tgcggttgcc
     1981 gacgccgcca tcaggcaggg aatcggcgac gccgaagcgc tcgcccggca cgtatatgaa
     2041 ggcgtactgc tggcctcact ggtgcacgag atcaacgccc tgcgcggcct gctgggcgag
     2101 cccgacgtgc tcgagcatgc cagcctgcgg ccgggcagcc tgtcgctcct aatgcgcttc
     2161 ggcgccctgc ctgtgacgct tgaccgggtc gacctgccgg gcatgacgcg ataccggatg
     2221 gagtttgctc ttcatggtcc gacgcggcga acgaccctgt ccttcccctc ccccttcctg
     2281 cgcaacgagc cgacgacgat ccgcattgag gagggcgctc cgggcacgac gagatcgagc
     2341 gtgcgcgagg agatcacgag ctacgagagc ggcttcaagc gcgagctgat cgcatttcat
     2401 gaggcgataa ccaccggcgc gccgatggag actccagggt ccgatgcagt ccgtgacatt
     2461 gcgctctgtc aggcaatcat tgccgcccat gcgaccggcg cgcctgtcga gcggccgaca
     2521 gagatgcggt gaccgtccga ctcgccaacg cgccggtcag ctacggcgtc tttgagctga
     2581 cgctcgattc ggatcgtttt ttggcgccgg cagaacgcgt cctcgacgag gtgcgcagcg
     2641 cgggctacgc gggcatcgat ctcgggtcgg tcggctacct cggggacgcg gccgggctcg
     2701 gcgagcggct ggccgaccgc ggcctcggcc tggccggggc gtacatcgag ctacgcttcg
     2761 aggaccgcat tgcgctcgac gccgggcggc cgtacctgga atcagcgctc gacatgttcg
     2821 acgcggcgat ccgcgctgac gccggccaac atccggcgcc gcggccgaca ctggcagcgg
     2881 ccagcagccc gtcccaccag cggcggcccg gtggagcgca gcatgatcgg tcgatcggcc
     2941 tcgatgacag cgaatgggca cgcttcgtcg acgggctgca gagcgtcgcg gcgcgctgtc
     3001 gcgagcgagg ctacgagccc gccttccacc accatgcggg cacggcgatc gaggcgccct
     3061 gggagattga gcggctgctg gacacgtcgg acgtcggcct gtgcctggac acgggccacc
     3121 tgaccgtggg cggcggcgac cccgtcgcag acctgctgcg ctgggggccg cgcatccgac
     3181 agctgcacct caaggacgca cgacgcgccg acctcgacca gatcgcggcc gacggaggat
     3241 ccgcgaccga gatctggacg cgcggtgttt tctgcccgct cggcagtggc gatctgaacg
     3301 tcgccggtgt cgtggacgca atcgtcacga tcggctatga cggctggatc gtcgtcgaag
     3361 aggacgttct gatcgccgac gaggcgggct tcgaacgtgc cgccgtgcac cagcgcgcga
     3421 accgcgtgct acttcagcgg ctgggcctct gagccgggcc aacgagcgcc ggttaactcg
     3481 atcactaaac cctcgtttgg acccagcacg aacgtttcgc ccagtcgctc gccggctcga
     3541 cgttgatggt cggttgccgc aagcacgcgg ctggcgttct cacagcgcat cgtccgcttg
     3601 gcgtccgcga aattgagcgt gatcagaaca tcgggcgcgc cgtcggcgcg gcgcagatag
     3661 gcgaagacgt ctggcggcgt accgtcaagc ggcaggtaat cgccgtgcgc cagagccggg
     3721 ttttcgcggc gtagcgccag gagccggcgc gtcaggttga gcagagatcc gtcatcggcg
     3781 cgctgcgccg cgacgttgcg ctgctcccac cccggcgcga ggggcagcca gggcgcgacg
     3841 cccgcgcgac agaagcctgc attgggggaa ccgtcccagt gcatcggcgt tcgctctggg
     3901 tcgcggctgt agccgggctc ggtgatgccc cacgtgtcgc gtacagcctc tggcgagatg
     3961 atcccattct ccatgccgat ctcgtcgccg tagtagatcg tcggcgcgcc gggtagcgtc
     4021 agtagcagca tcatcgcgac cggcgcctga gcaatgccga cacgcgacgc cacccgcgcc
     4081 tgatcgtggt tgcctagcac ccaactggcc cacgcgctgc gcggcagcgc ggcgacgacg
     4141 ccgttgacga cgttgcgcac gctcgatgca tcccacgacg catgaagcag gtggaagttg
     4201 aacggcagat ggatctcgtc gaggttctcg ccgtagaaac cggcccatac gtcgaggtcg
     4261 ggatggtgca gctcgccgat cgtgacgcgg tcgcccgggt agctgtcgac caggctgcgc
     4321 acgtcccggt agatctcgtg gaaatctggg tgagcgtggt cgtacaggtg cacctgccgg
     4381 ttccactcgc tcagatgggc aacttcgccc ggggcgggat gcggcgggtt gtcgcgcagg
     4441 agcgggtcct tggctacgaa gttggcgacg tcgatgcgaa agccggcaac gccgcggtcg
     4501 agccagaagc ggagcacatc gagcatcgcg gcgtggacct cagggttgcg ccagtccagg
     4561 tcgggctgct ctttcaggaa cgagtgcagg taatactggc cggtcgtcgg gtcccgctcc
     4621 caggcactgc caccaaacat gctgacccaa ttgttcggcg gcccgccgtc aggtcccgga
     4681 tcgcgccatg tgtaccaatc acgcttcggg ctgatgagcg aggagcggct gtcgaggaac
     4741 cagcggtgct gatcactggt gtggttgggc acgtagtcga cgatcacgcg gatgccgcga
     4801 cgctgcgtct ccgcgatcaa gcggtcgaaa tcggcgagcg tgccgaatag tgggtcgacg
     4861 tccgtgtagt cagcaacgtc atagccaaag tcggccatcg gtgatcggta gaagggcgaa
     4921 agccagatgg catcgactcc cagccatgcc aggtgttcaa ggcggccgat gatgcccggg
     4981 agatcgccga cgccgtcgcc gctggcgtcg gcgaagctgc gcgggtagat ctggtaagtg
     5041 gcgcccgact gccaccacag tggagggcgc tgcatcatcg ctcctcgacc agtcccgacg
     5101 ccgcgcgcgc gcgctcgtag gcggcgcgcg ccctcactac ggccgcagag ctcgagactt
     5161 cggcgacggg tacgtcccac cacccgtacg acggcacctc gttcgccgga tcgacctcga
     5221 cgacgacgac cgtggtgcga gcaaactcgc gcgcgccgtc gagtgcggcg cgcagctccg
     5281 cgagcgtctc tgcgcgtacc gcgtgcgcac ccatgctctg ggcgtgggcg cgatagtcga
     5341 ccggcgtcgc caggagattg aagtcgttgc gtgctccgag cgagctcgca agcgcgctga
     5401 tcgatcggta gccgtggctg tcgacgacca cgaccgtcag ccgcaggccg tgctcgacgg
     5461 cggtcactat ctctgagttc atcatcaggt agctgccgtc gccgaccagg acgacgacct
     5521 cgcgcgccgg ttcggcgagc ttgaccccca ggccgccggc gatctcgtag cccatgcacg
     5581 aatagccgta ctcgacgtgg taatcatcgg gcgtcgcact ccgccacagc ttgtgcagat
     5641 cgccgggcat gctgccggca gcactgatga tcgtcccgct actcccgctg aactcgttca
     5701 ccgcagcgat caactgggcc tgtgtcggcc gcccgctccc ggtcgtgccg ggcgcggtca
     5761 ggctcgtcgc cagcgcgtcc cattcggagc gcagcgcgtt gacgcgcgtc gtgcgctcga
     5821 cggcgcgtgg ggtccccggc ccgtccattg agcgcagcct cgagctcaac gcgcggagtg
     5881 cctggcgggc atcggcaacg accgcgatcg cgccgacctt gtgagcgtcg gccgcatcga
     5941 cgttgatcgc gacgaaccgg accccgggtg tcgaccaggt cgtaccgctg gcggtcgtga
     6001 agtcgcccaa gcgcgtgccc agcgcgatga ccaggtcggc gtcggcggcg tagcgattgg
     6061 cggccagccc acccgtcgcg ccgatcgcgc cgaggttcat gggatgctcg tgtgcgagtg
     6121 tcgagcggcc ggcctgggta agcgcgaccg gcagcgcgaa ctcctctgcg aactccgcaa
     6181 gatcgttctc agctgccgaa tagcgcacac cgccgccagc aacgatcatc ggccgctcgg
     6241 cggccgcgat gagccgcgcg acgtgatcgg cgagttgcgg ctcgggctcg ccacgcggca
     6301 catgccacgt ccgtggccgg aggaacgact ctggccaggc gtaggcctcg gtctgcgtgt
     6361 cctggggcag cgcgatcgtg accgccccgg tctccgccgg cgacgtcagc acgcgtagcg
     6421 cttcgggcag cgcgtcgagg agctgctcgg gccgcatgat ccgcgtccag aagcgactca
     6481 cgggtcgtag cgtgtcgttg accgacacgt cgcgaccggc gcgcgactcg agctcctgga
     6541 gcaccggtcg cgcgctacgg ccggcgaaaa tgtcgcccgg caggagcagc accggaagcc
     6601 ggttgatggt cgcgccagcc gcgccggtga ccatgttggt ggcccccggg ccgaccgacg
     6661 tggtgcaggc aagcgctccc agccggcggt gcatccatgc gtagccggcc gctgcgtgga
     6721 ccatcgcctg ctcgttcctg aagggcatga agcgcacgcg gtcgtgctgc ccctcgagag
     6781 cctcgccgat gccgccgacg ttgccgtggc cgaagatgcc caggacgggc ccaaagaagg
     6841 gcgtgacgcg accgtctcgc tcgacgttct ggccgcctag ataagcgatt agtgcctggg
     6901 ccgcagtcag ccgcaggctc gatccggtca acgccgacga ttgtaaggtt aggcacgatg
     6961 ccacaagccg tgatccatgg tgcggccgtg ccgagcacac tctccgctga cgagctgcac
     7021 ctccgttcgc acgcgggcgg gatcgaggtg gatccaccgc gcgtcggctg gcgctacctg
     7081 tcgtttcgtg cactccattt ggacggctcg ccgtattcgc tcgacccagc gctcgagcat
     7141 gccgttgtcg tgatcgccgg cgcaggcgtc acggtcgtcg acgcgagcgg cgacggcatc
     7201 gaggtggcgg gccggccctc gccatttgat gctctgcctt cggcgtgcta cgtcccggcg
     7261 ggcgagccga gtgagctggt cggcagctcg tcgctcgtcg cgatagccag ttcaccgact
     7321 tctggtcgca gcggtgttgc gcgtgcacgg gtggcgattc gtccgagcga cgtgcgcatc
     7381 gagattcgcg gcgcgggcaa cgcgacacgg cagattaacc acatcatcag ccccgacttc
     7441 cccgccgatc ggctcgaggt ggtagaggtc ctgacgccct cgggcaactg gtcgagttgg
     7501 ccgccgcaca agcacgatgt cgacgacatg cccgcagagg cagtgctgga ggaggtttac
     7561 tactaccagt tccggcgacg cgaggcgtgg ggcatccagc ggctgtatcg gcgcgaccgg
     7621 tcgcgcgatc acgcctggga ggttcgagca ggtgaggcag tgctcgtgac cgatggttac
     7681 caccccttcg tcgcctccca cggcgacgac gcgtactacc tcaatgccct cgccggcgac
     7741 cggcggacga tggcgtgctc attcgacccg gactacgact gggtccgcgg aacgtggggt
     7801 gccgtagagc cggacacacg cgtcccactt gtgcggtgag cgacgtgccg gatacgagtg
     7861 aggtggcgat cacgacgatc cacttcgtgc cccacaccca ctgggaccgc gagtggtacc
     7921 agccgttcca ggttttccgc atgcgcctga tagcgctcgt cgatgacgtc ctcaatcacc
     7981 tggagcgcga gccggcgttc cggttcacgc tcgacggaca gctggcgacg atcgacgatt
     8041 atctagaggt caggccgcag gccgaggagc gcatccgcca tttcgtggcc gagggccggc
     8101 tggctatcgg cccctggcag atcttgatgg acgagttcct tgtctccggc gagaccatcg
     8161 ttcgcaacct ccagcgcggc tggcagcggg cactcgcttt tggcgcgccg atgtccatcg
     8221 gctacctgcc ggacatgttc gggcacatcg cccagatgcc gcagatcctg cgtcgcgcgg
     8281 gtatcgatcg cgccgtggta tggcggggag ttccggcgca ggtgacgcgc aacgtcttcc
     8341 gctggcgcgc gccggacgga tccgacgtgg tcaccgagta cctggtcgga ggctatggca
     8401 acgcggcata ccttcttgag gtacccggcg ccgccgccga caaggtgacc ggctaccgga
     8461 aggcgttcgg cccctggtac ggcgacgagt ccttgcttgc catgtacggc accgatcaca
     8521 tgtcgccggt gcccgagatg gtcgcgttgc tcgaaggcgt caacgccgct gattcaggcg
     8581 tcaaggtggt gatcgagacg ctcgccgatt acatggcggg gcgcggagac ggggacgggg
     8641 acggggggca tctgctcgtt gagggtgagc tgcgctcggc cgcccactcg aacatgctga
     8701 tgggcgtcaa ctccgcgcac atcgacatca aggcggccgc cgctcgggcc gagcgagggc
     8761 tgagccgcta cgcagagccg ctggcagcgc tccacggaga tgagtggccg gagcgcctgc
     8821 tggagctcgg ctggcagcgc gtgatcgact cttcagcgca cgactcgatc tgtggctgct
     8881 cggtcgatcc ggtggccgcg caggtgctcg tgcgtctgga tgaggccgcg cagatcgcgg
     8941 gcgagatcgc tgcgcagacg gcgcgtcgcg tcgccatgtc ggttccgcat ggcgactggc
     9001 tgtggctcaa ccccacgcct gcggctcgat ccggcctggt cgagctcgac gtgcttgccg
     9061 ccgacgacac gcccgaggtc gcgctgcagc tcgccgatgg gcggctactg gcgacccagg
     9121 aggttgatcg caacagcccg ctcatgctgg agaccgagct ccgggcctca gaagtcggta
     9181 cgttccttcg tcgccgcatg cacggccgcg agctgttcgg caagcagctt aacgccgcga
     9241 cggtgaccag cgggacggtc acgctcgtgc tcgacagcga ggcggacccg gagtggctcg
     9301 acgtcgatca gctgcggacc gagatcgagg cggcggtgac ggccgcgccg gaagagaact
     9361 ggcgagtgcg ggtcatcagc gccgggcgca ggagggtcag agccatggtg ccggcaccgc
     9421 cactcggatg gtcgtccggt cgcccggtcg gcggcagcgg cacgctcgag gagccggtgc
     9481 gcgtgtccgg gcgctcgctc gacaacgggc tggtaagcgt gtcggtagag gccgacggaa
     9541 cactgacgat cgtgcgtgac agcatcaccg tcagcggcat cgggcgcctg gtcgacggcg
     9601 gcgacgcggg cgacagctac aactacgctc caccgttcga agatcgcatg atcgagtcat
     9661 cgggcacgca gccgaccctg atcatggcga gcgggccagt cgtcggcgag ctgctcatcc
     9721 gacgcgcgta tgccttgccg gtcggcctga acgccgatca ccggagcagg tccgcgcgca
     9781 ccgtcgagac ggaaatcgac atgcacgtcg tgctgcgcgc cggcgagccg ttcgttcgaa
     9841 tccggctggc cttcgacaac caggcagacg atcaccggct ccgccttcat ttgccgctcg
     9901 tcgagccgac cgaccactct tcctcggaag gccagtacgc cgtcgtccgg cgcggcctga
     9961 cgtgcgaggc cggccacggc gaggtgccgc tgccaacgtt ccctgcgtac ggctgggtgg
    10021 acgcgggcgg cgtgggcata ttgctcgacc acgtcaccga atacgagctc atcgacggct
    10081 cacagctggc gctgacatgc ctacgttcga tcggcatgat cagccgcaac gacaacccgt
    10141 accgcgagga tccggcaggg ccggagcggc ccatgcccgc ggctcagcta cgcggccggc
    10201 gtgagttctc catcggcgtc ctcgtccacg gaaaagcgtg gcacgcggcg gacgtcatcg
    10261 gcgccgcgga gcagtacgca cacgacttcg tggtcgcatc gggcaccaag cgtcgcgatc
    10321 gcaccccagc agaatcaagc ggtggcttga gcgtgtccgg ctcgggcgtc gttacgacgg
    10381 ctctgcgccg ggtgggcgat tggctggagc tgcgcgtcgt ggctgaatcg ccgacggcca
    10441 ccgaagcgac gatcggcgac ggactcaccg cagcccgcga agtggacctg ctcggccgca
    10501 tcgtggctgg cctggcgatc gccgacggct ccctgcgtgt gccgctcgag ccatggcaga
    10561 taaggacgat ccagctccac cgctgatcac gcagggtcgg cgaagcgaag ccgcgcgatc
    10621 accggccagt gatccgacag atcgccagcc tctcgcgcca cctggcactc ggtgaactgg
    10681 aagccgcgcg ccagcacgtg atcgatgcgg tcgacgccgc acgattcgcg gcgcgcatcg
    10741 ccgaccggga tgccgactga cgcaaagccg tcgtcaagga tcgtcgtcag tgccctcagc
    10801 tcaggtcgtt cgacagctgc attcaagtcg ccggccagca cgagcgggcc gccggtcgcg
    10861 tcgacaatct cggcgagccg gtctgcctgt aagcgacgcg cgcgcgagcc caggtaagcc
    10921 aggtgcgtcg acatgaaggt cagacgcccg tctggcgtgg cgatctcgca tgccagcgcg
    10981 cagcgcggct cggtgtcgac cgggtcggtc gggtcgtcgt ccgtggccac cggcagggca
    11041 tgggtggtcg cagcctcgat cggatgacgc gaaagaatca cgttgcccca cagcgttgcg
    11101 ccgacaactc gccgcgagtc gggctcgacg agcggatagg ccgacactgc ggcgtagcga
    11161 acctccaggc cggtcaggtc ggcgaagaag gcgggctggt cgaccgccag agcctcgacc
    11221 gatgccagcc cgacctcctg gagcgccacg acgtcggcac cggcaagagc gatgaaatcg
    11281 gacatgcgac gcaggcgact gtcgtcgatg cgccgccacc aagcgggggg aaacggccca
    11341 ggaccgatgc ccgcgcgaac gttgtagctg acgacggtca gcggcgatcg gctgcgcgaa
    11401 acgcctcccg cagctccgcc cgccacccgg cggctacttc acagatccgg cgaggagtcc
    11461 ctggacgaag tagcgctgga gggcgaagaa cacgagcagc ggcactgtca tcagcaggaa
    11521 tgctgctgcg gccagcagat gccactcgct gccgtacgtt ccgagcagcg agttgacggc
    11581 caccgtcatc gggtgcagcc gaccgtcctg gatgaagacg agtgccatca gcaagtcgtt
    11641 ccagacccac aggaactgga agatgccata cgcagcgatg gccggcacgg acaaggggac
    11701 gacgatcgtg cgaaagatgc cgatcgtgga tgcgccgtcg atgcgtgccg cctctatcag
    11761 gtcgcgtggc agggtgatga agaagtttcg caacagaaat atgccgaacg gcagcgcaaa
    11821 ggcagtgtga gtcagccaga ccgcggcata gccggtgagc agcccggtcg gccggaacat
    11881 gagcaggagg gggatgaatg cgagctggac ggggaccagc agcagcgcca cgacaagcag
    11941 gaaaagcgtg tcgcggaacg ggaagcgcag ccacgaaaaa gcatatgccg ccatcgagca
    12001 caggacgatc ggcagcagcg tcgacggtac ggcgatgagc aaggtattga tgaatgcctg
    12061 cgtcagcccc tccgcttcga gtacaaaacc gtagttctcc gtcgtgaagc ggagcgtgaa
    12121 gatcgactcc caccagcccg tttcctgaat gtcgccgcgt ggtcgaaacg acgtcacgag
    12181 caggccgacc gtaggcgcga gccagactag cccgacgagc gccagggcga tgtggatcgg
    12241 ggccctgttg agcaggcgaa ggacgcgacc gctgagcgtc tccgctctgt ggtgttcgtc
    12301 cgtgcccgcc atcgccgcgt cgcccgtcac gtcacggcct cagatcggaa gcggcgcacg
    12361 ttgtagaaca tgatcgggat gaggatcagc atcatgatga cggccaccgc ggctcccttg
    12421 ccgtacaggc cgcccggtat cgcctcctgg tacatggtga aggcgataac tcgactcgaa
    12481 actccgggtc cgccgcgggt catcacgaag atcaggtcga acagcttgat gacgttgacg
    12541 atgagtgtca cggcgaggac cgacatgggc aggctgacca tgggcacgat gattcggaag
    12601 aagatgtggc gctcggtagc gccgtccacg cgcgctgcct cgaggatctc ggtcggaatg
    12661 gctttgagcg ccgccgagag gatgactgtc gcgaacccga ccgaacccca tatccctgcc
    12721 gcgatcagtg cgaagttgac gaggtctgga tcgcccagga acgaaactgg cccggccgtc
    12781 accggatcca gaattgcatt gagcagcccg gtctgcgtgt ccggcgcgta gacgaagcgc
    12841 cagatcaccg ccagcgccgt ggccgcgatg gccatcggca ggaagacgat ggccttgatg
    12901 accgactcgt agcgcacccg cgaagcgagc acggcgatga tcagcccgag cacgatgacg
    12961 atgctgacgt acagcaggat ccacagcatg ttgttccaga tggcacccga gggcgggaac
    13021 gtgctgaggt tgatgaagct ccggtcgttc agcaggctca cgtagttgtc gagccccacc
    13081 cactccgtga actcgccgcg ccggccgcga ttgaagctca gcagaaacgt gtaggaagtg
    13141 gggatgatga tgaacactac gaggaacgcc gcgggcagcc cgatgaagaa gatggccgac
    13201 agatcgaagc gccgaccgga cggccggacg ggcttcggct tgacgacgac gccgacaggc
    13261 gcggcagtgg ccataagtcc ttacctctcg ccgaaacgaa ggcgcgggcg gcctttcggc
    13321 cgcccgcgtc ctctctgact cagaacttat tgctggtccc tattcggctt cccagcccgc
    13381 ctgaacgcgg gactggtagt cggcccagtc gatcgtttcg ccgcgcaggg cccgctgcaa
    13441 cagcgcaccg agactctcgc cggcgacgcc ggccgggagc aggtccgagc cgtcgaaacg
    13501 gatcgcctct gcgttagcca gctgctcggc ctccttgact acaagctcat tggggtaggc
    13561 gtccgtgccg acacccttgt gcggcgacac gatggcgccc gtgcttgccc atgcagtgtt
    13621 cgcctcgggc gtgatgatga actgcatgaa cgcctgcgca gccgcgctgt tgctgaacgc
    13681 gccgacgacg tcgccaccga acgttgtctg atcggcggtg ttattgatcg acgggaacgg
    13741 gaaccagtcg atcgtttcac caatcaccag attcgggttg acgtcgcccc gggcgatgcc
    13801 acccacgaaa ccgccctcgt aatagatggc agcttcagga gtctcgctga acgcctgggc
    13861 gatgccatca acgaacccac gtcccagcgc ggcatcgacg ccgccggtga catagttctc
    13921 gttgagcacc tcaagcatcg tgtctaccgc cgtctggacg gtcgggtccg accaatcgcc
    13981 gtccgcgctg aacagcgtgt cgtacgcctc aggaccggcc tgacgcaggt agatgctctc
    14041 gaaccagtcg gtgagcgtcc agtcgtcagc ggcaccgagc ccgagcgcac caggtgtgtc
    14101 tcgcagcgcg tccagcgctg ctttgaactc ttcccaggtg gtcggcacct cgacgttggc
    14161 ggccgcgaag agatcgggcc gataccagat cgtacccttg ctgttgtact tcgccggcac
    14221 accgtagacc tcgccgttga cggtcccgga gtcaacgaat ccctgagggt aaagctcacc
    14281 aaggctggcg atgtcgacgc ccagctcgct caccggcacc agtgactgat cagccgcgaa
    14341 gcgtcgcagg aaaccaatgc ccgggatgat cgcgatgtct ggtgcgttgc cgccgctgat
    14401 gcggctctgg agcacaccgg catagtcggt gcgctgcgtc tcgaagttgt acgggatccc
    14461 cgtttcggcc gtgaacgcat cgagcacgtt caggaacgct tcgcgttcgc tgccgcccca
    14521 gagcgagaga accctgagcg cgccttcgct cggtgcttgg gactcgggcg cctggctctc
    14581 tggcgcctgg ctctctggcg cctggctcac ggacgcgcta gccacagcac cgcccgtcgg
    14641 ttggaccgtc gcgcccgggc ctccgcacgc caaaacgacg agcgagagca ccgttatcgc
    14701 agcgagccag cgaatggccc gcgaccgggc cggatggatt tgatccaccg cttcctcctc
    14761 ctattctcgg cgtcgagtct taatcctggc ccgacgagcc gttgcctctg ggcgcaacca
    14821 catgtgccag tttcgttcgt tctgtcagcg ccttccggcc acgcctcggc cgtagcctac
    14881 tcggcgtgcg cgcgacgggc aaggcgttcg cccaccgttt cgttgagggc gtcatcgccc
    14941 gcttgtgagc gttccagcat gtttcgacga ccctcctcct tcgatcggcc agcgcaggga
    15001 ttagccggcg ctgtcgactt aaggctgctg ccccggtggc gggtcacgct caaccgccgt
    15061 gacgggatcg ccctcaagct tgaaaacatg ctcgtcgcgg cgcaaccaga acagcggcgg
    15121 cagcgcgatc gccatgaaga tcaacgttgc catgagcaac ccatcgatcc cggccgcgtc
    15181 ggacgcgatg ccgccggcca tggtcccgac gatctggccg actgccagga agacgccgta
    15241 gaggcccatg accgcgccac gatcgtcggg gtaggcctcc gacatgtcgg cgagcaagcc
    15301 gagggcggct ggcgtcgcgc ccgcgagtac gaagaggccg gcgcccgcaa tgacggccag
    15361 aggcagacga aggatgggct caatgccgcc tgagtggttg aacgcgatgg cagccgcgac
    15421 cagcgcaaat ccgccaacga tgccgtagaa gatgatcgtc gtgcggcgca tgttcttgaa
    15481 gcgattgccc cagtagatga cgccggcgac gaagacgagg cccaccgcag cgaggccgac
    15541 cgaaatctcg acggggctca ggccaccagc gaggagctgg tccgggtagc gacccgactg
    15601 gtcgcgaacg aggttgaaga gccccgtgcc ggcataaacg ccgagcgcgg cgttgacggc
    15661 gatccacgtc ggcgccaaca gccagatgtg cgatgaccgc aggagc
//
LOCUS       DATLCO010000062         4554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3800542, whole genome shotgun sequence.
ACCESSION   DATLCO010000062 DATLCO010000000
VERSION     DATLCO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3800542"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VMZ33_01640"
     CDS             <1..907
                     /locus_tag="VMZ33_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR15963.1"
                     /translation="DEVQAKVKAAGASAGYELTTYTQGYVDLYQELDLLLPLDESKLS
                     NLDGLYPYFADDANNYWTDADGQRIGVPWTWGSAGITYDSAKIGEMSSWYDLLDPSLK
                     GKIALPDDPQGHFALTTRILGLHPDTCPKDRLDDVSALSAKFVAQSKGVSPSYGDMTT
                     KLVSGEAVACYQGWAAMNSFAAGEGLMTVKTNVPKEGSYSFCDAWAIPRGADNVDTAL
                     AWINETLSAKTNAAAAEYLVGGVTIQTSLGYLNKETKALYSYDDLDGLLEQAPFNHNT
                     PTESADYVTFKEWNDRWQELKAGRG"
     gene            924..1814
                     /locus_tag="VMZ33_01645"
     CDS             924..1814
                     /locus_tag="VMZ33_01645"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR15964.1"
                     /translation="MKPGTATRGLVGRAGSLTLSMPAAAGMLLFFVGPMALFLTYSFL
                     TAGLYSVSGPLTIDSYREAIDSEFNQALARNSLIIGVTTALVTVAFAIPIAFWLRYSA
                     GRWRMPILFLITASMFASYLVRIYAWRSILGSNGLLNSGLERLGLTDEPLGFLLYNRF
                     AVTIALVHIFLPYVVLVVYAGFAPIPSALLEAARDLGAGAFQRWRRVVLPLIAAPAAS
                     SFLFVFVLSAADYVTPQFLGGANGLMLGVQIQTSFVSTGNWPLGAALSFLMLAGFVGC
                     YAAVALALRLLKLDRIVVAH"
     gene            1814..2620
                     /locus_tag="VMZ33_01650"
     CDS             1814..2620
                     /locus_tag="VMZ33_01650"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR15965.1"
                     /translation="MEQPQRSRVTALITAAAVVFLFVPLVLVVLFSFHSTGLSFPFRG
                     FSLRWYEEIFSSHEFRTAALNSLIVATSTSLITLIAGTLAAYGLSRTASRLRGPLALL
                     FFLPITLPGLFLGLALLVSFSRLQVKLSLATVTIAHLVYVFPYYVLISRAALDRLDRG
                     LEEVAADLGANAMLVFRRVTLPQIWPVLLGATCLAFALSFDEFVITFFVVGEDSTLPL
                     FIWSRLRRTIDPSINAVSTLLLLSTLLLFAFAFALALRGERLRSGRTAPL"
     gene            2659..3711
                     /locus_tag="VMZ33_01655"
     CDS             2659..3711
                     /locus_tag="VMZ33_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR15966.1"
                     /translation="MQRFGREVVALRGVSLDVLEGEFITLLGPSGCGKTTLLRVIAGF
                     ETPDEGRIVLEGRDMTSTPSHRRPVNMVFQRPLLFPHLDVFENVAFGLRIAGTDRAEV
                     ERRVAEALRLVRLEGFERRRSHQLSGGQMQRIALARALVNRPKVLLLDEPLSALDLKI
                     RLEMEAELRRVHRETGATFIYVTHDQREALALSDRIALLNDGRIEQVAGPREIYDAPT
                     SPFAARFVGDANLLPVDVVVRGGETTVLLAGATVPAAATGAASGSAWLVLRPEAISVS
                     ADRGTPDGLAGTVVDAAFRGTGFDYRIRVDGIAELVKAEPPRREFVPLGSRVRLNWEI
                     SDAALLPRADTDTEPW"
     gene            complement(3771..3845)
                     /locus_tag="VMZ33_01660"
     tRNA            complement(3771..3845)
                     /locus_tag="VMZ33_01660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3786..3788),aa:Ser,seq:cga)
     gene            4134..>4554
                     /locus_tag="VMZ33_01665"
     CDS             4134..>4554
                     /locus_tag="VMZ33_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15967.1"
                     /translation="MAKELASYPDEVATRVRESIERFRNYDNIDEPFCPRHDVLTLPS
                     AILDGRSLAAALTPSPNTVQGNNPFSFTLVDYEVPPARTTKNSRFMLNRFEDGRASTS
                     TMSIDLLLRHEDGTLIVGEAKVAKIEGYDTDSVLALVQ"
BASE COUNT          719 a         1599 c         1400 g          836 t
ORIGIN      
        1 ggacgaggtt caggccaagg tcaaggccgc gggagcctcg gccggatacg agctcacgac
       61 ctacacccag ggttacgtcg acctctacca ggagctcgac ttgctccttc cgctcgacga
      121 gtcgaagctg tcgaacctcg acgggctcta cccgtacttc gccgacgacg cgaacaacta
      181 ctggaccgat gcggatggcc aaaggatcgg cgtgccctgg acctggggct cggccggcat
      241 cacctacgac tcggccaaga tcggggagat gagctcctgg tacgacctgc tcgacccctc
      301 gctgaagggc aagatcgccc taccggacga tccccagggt cacttcgccc tgacgacgag
      361 gatcctcggg ctgcacccgg acacgtgccc gaaagatcgc ctggacgacg tcagcgcgct
      421 ctctgccaag ttcgtcgcgc agtcgaaggg cgtctcgccc tcctacggcg acatgacgac
      481 gaagctcgtc tccggcgaag cggtcgcctg ctaccagggc tgggccgcga tgaacagctt
      541 cgccgccggg gagggcctga tgacggtcaa gacgaacgtc cccaaggaag gcagctattc
      601 gttctgcgac gcctgggcga ttccccgggg agccgacaac gtggacacgg cgctggcctg
      661 gatcaacgag acgctgagcg ccaagaccaa tgcggcggcg gccgagtacc tggtcggggg
      721 cgtcaccatc cagacctccc tcggctacct caacaaagag accaaggcgc tctacagcta
      781 tgacgatctg gacggcctgc tcgagcaagc gccgttcaac cacaacactc cgaccgaatc
      841 ggcggactac gtcacgttca aggagtggaa cgaccggtgg caggagctca aggcgggccg
      901 cggttgagac tctgcaggcg ccggtgaagc cggggacggc gacccgcggc ctcgtcgggc
      961 gcgccggatc gttgacgctc agcatgccgg ccgcggcggg catgctcctg ttcttcgtcg
     1021 gcccgatggc actgtttctc acctacagct tcctgaccgc cgggctctac agcgtgtccg
     1081 gaccgctgac gatcgacagc taccgcgaag cgatcgactc tgagttcaac caggcactgg
     1141 cccgcaactc cctcatcatc ggagtcacga ccgcgctggt gactgtcgcc ttcgcgatcc
     1201 ccatcgcctt ctggttgcgc tacagcgcgg ggcgctggcg catgcccatc ctcttcctga
     1261 tcaccgcgtc gatgttcgcc agctatctcg tcaggatcta tgcctggcgc agcatcctcg
     1321 ggtcgaacgg actgctgaac agcggcctgg agagactggg tctgaccgat gagccgctcg
     1381 ggttcctcct ctacaaccgc ttcgcggtga cgatcgccct cgtgcacatc ttcctgccct
     1441 acgtggtgct ggtcgtctac gccggctttg cgccgatccc ctcggcgctg ctcgaggcgg
     1501 cgcgcgacct cggagcggga gcatttcagc gctggcggcg cgtcgtcctg cctctgatcg
     1561 ccgcaccggc ggcgagctcg ttcctcttcg tcttcgtcct ctccgccgcc gactacgtga
     1621 ccccgcagtt cctcggcggt gcgaacggcc tcatgctggg cgtccagatc cagaccagct
     1681 tcgtctccac gggaaactgg ccgctgggcg cggcgctgtc gttcctgatg ctcgccgggt
     1741 tcgtcggctg ctacgcggcc gtcgcgctcg ccttgcgcct gctgaaactg gaccggatcg
     1801 tcgtcgctca ctgatggagc aacctcagcg gagccgcgta acagccctca tcaccgccgc
     1861 cgcggtcgtc ttcctgttcg ttccgctcgt cctcgtcgtc ctgttctcgt tccactcgac
     1921 cgggctctca ttcccctttc gtggcttctc cctccgctgg tacgaggaga tcttctcgtc
     1981 acacgagttc cgcacggcag ccttgaacag cctgatcgtc gcaacctcga catcgctgat
     2041 cacgctgatc gcgggaacgc tcgccgcgta cggcttgtct cgcacggcgt cccggctgcg
     2101 cgggccgttg gcgctgctct tcttcctgcc gatcacgctg ccggggctct tcctcgggct
     2161 cgcgctgctc gtctcgttct cgcgtttgca ggtcaagctc tcgctcgcca ccgtgacgat
     2221 cgctcacctc gtctacgtgt tcccctacta cgtcctgatc tcgcgcgccg cgctcgaccg
     2281 gctcgaccgt ggcctcgaag aggtggccgc ggacctggga gcaaatgcga tgctcgtgtt
     2341 ccggcgcgtc acgctgcctc agatctggcc ggtgctgctc ggcgccacct gccttgcgtt
     2401 cgccctcagc ttcgacgagt tcgtgatcac cttcttcgtc gtcggggaag actcgacgct
     2461 tcccctgttc atctggtcgc gcctgcgccg gacgatcgac ccgtcgatca acgccgtctc
     2521 gacgctgctg ctgctctcga cgctgctcct gttcgcgttc gcattcgcgc tggcgcttcg
     2581 aggcgagcgt ctcaggtccg gccggacggc ccccctgtga gtcagaccac cgagcctcag
     2641 gttcggctcg aaggcgtcgt gcaacgtttc gggcgcgagg tcgtggcgct ccgcggggtc
     2701 tcgctggacg ttctggaggg ggagttcatc acgttgctcg gtccgtctgg ctgcggcaag
     2761 acgaccctgc tgcgtgtgat cgcggggttc gagacccccg acgaagggag gatcgttctc
     2821 gagggtcgtg acatgacgag cacgccgtcc caccggcgcc ccgtgaacat ggtctttcag
     2881 cggccgctgc tctttcccca tttggatgtg ttcgagaacg tggccttcgg cctccgcatc
     2941 gccggcaccg atcgcgccga ggtcgagcgc cgggttgcgg aggccttgcg gctcgtcaga
     3001 ctggagggat tcgagcgccg gcgcagccat cagctctccg gaggccagat gcagcgaatc
     3061 gccctggccc gcgcactcgt caatcgtccc aaggtgctcc tgctcgacga gcccctctct
     3121 gctctcgacc tcaagattcg gctggagatg gaagccgagc tcagacgcgt ccaccgcgag
     3181 accggcgcca ccttcatcta cgtgacgcac gaccagcgtg aggcgctggc gctctccgac
     3241 cggattgcct tgctcaacga cggccgcatc gagcaggtcg ccggcccacg cgagatctac
     3301 gacgcgccga cctcgccctt cgcggcacga ttcgtcggag acgccaacct cctgcccgtc
     3361 gacgtcgtcg tccgaggcgg agagaccact gtcctgcttg ccggggccac ggttcctgct
     3421 gcggcaaccg gcgccgccag cggcagtgcc tggctggtcc tacggccgga agcgatcagc
     3481 gtcagcgccg atcgaggcac ccccgacggc ctcgccggga ccgtcgtgga cgccgccttt
     3541 cgcggcaccg ggttcgacta ccggatccgt gtggatggaa ttgcggagct cgtcaaagcg
     3601 gagcctcccc gcagagagtt cgtaccgctc ggcagccgtg tccggctcaa ctgggagatc
     3661 tccgacgccg ccctgcttcc gagggcggac accgacaccg aaccttggtg aaccgtctgc
     3721 acgagttcga ttcttgccgg ggaaaaccgc cgtccagcgg ctttggggag agccgacgcg
     3781 cggactcgaa ccgcggaccc cttcattacg agtgacggca agccacggga ctcctgtcca
     3841 ccgccgtccc cccgcgggcc tagaaacaac ccgttagcgg gcttatggcc ggatccgaat
     3901 aggccgtcac gcccaaggcg tggacacgtc ttcgtaccct cctcgtaccc tcgacccaac
     3961 gacgcggcga ccctggtgtc atccgcacat gtgaaggtgt caagaggaag gggccgtaga
     4021 atcccgaggt gggcggcatt gtggctgacg gcctgaagcg tccgatcccg gtcactccgc
     4081 tgctctgcct ctaccgagcg atgcgagccg cctcacaagc cgagcggttg gaaatggcta
     4141 aggagctcgc gtcctacccg gacgaagttg cgacgcgggt ccgcgaaagc atcgagcggt
     4201 tccgcaacta cgacaacatc gatgagcctt tttgcccacg gcatgacgtg ctcactcttc
     4261 cctcagcgat cctcgacggc cggtcgctcg ccgcggcgct gaccccgtcg ccgaacaccg
     4321 tgcagggcaa caacccattc agcttcactc tcgtggacta cgaggttcca cccgcgagga
     4381 cgacgaagaa ctctcggttc atgcttaacc gcttcgaaga cggtagggcg tcgacatcca
     4441 caatgtcgat cgaccttctc ctgcgccacg aagatggcac tctgatcgtt ggtgaggcga
     4501 aagtcgccaa aatcgagggg tacgacactg actccgtgct cgcgctcgta cagg
//
LOCUS       DATLCO010000063         2491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1565498, whole genome shotgun sequence.
ACCESSION   DATLCO010000063 DATLCO010000000
VERSION     DATLCO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565498"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VMZ33_01670"
     CDS             <1..609
                     /locus_tag="VMZ33_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15968.1"
                     /translation="MEPEHISAYALALDSYAPSPDHVPVSRGASAWRARARGAQDQDR
                     AAAMYELADDEFGRAGMGWYEISNWARPGRASRHNNVYWQSESWAAVGPGAHAFDGVA
                     TRRWNDASLDAYLSALDKGWLPTGGSAVSDAASVAGERTMLALRTAAGVPRDASNMAL
                     TWGLDNDLLEGIGERVRLTRRGRLLSNELFVRLVPEAEAAAA"
     gene            complement(668..1378)
                     /locus_tag="VMZ33_01675"
     CDS             complement(668..1378)
                     /locus_tag="VMZ33_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR15969.1"
                     /translation="MTQPAPIILVPGWWLGAWAWDEVIVNLASEGVVATALTLPGLES
                     TDADRSSVTFEDHIAAISQAVEASERPVVLAVHSGAGFPGYATSDRVPERIAAMVYVD
                     TGAGVGAMDAEFADVEKPLASPEDLAANENLDGLSEQQLATFRERAVPQPGGVLRGST
                     ELANDARKAIPTVVICTAYTAEEYRDAVEKGYDFVAGLADQTDVTYVDLPTSHWPMWS
                     RPHDLAQILADVAREHAS"
     gene            complement(1394..1966)
                     /locus_tag="VMZ33_01680"
     CDS             complement(1394..1966)
                     /locus_tag="VMZ33_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15970.1"
                     /translation="MAPPLVWDGGPLGCTGPPPLPGPRTRLPPNYYISRLVEPGIGFQ
                     VGRGWTTEQQTPGFFDIQDDPGSPDVVAVQFANIVGAETAAEAAQQIAARENLVIDGP
                     TDVEINGRPAVRLTVESTDPLDTDPPAFHQVLTVAAGPLSIASARRLQINLLDIDGGV
                     LAILVGGSVEKWDRAVRVSAPVLESVFILD"
     gene            1983..2231
                     /locus_tag="VMZ33_01685"
     CDS             1983..2231
                     /locus_tag="VMZ33_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15971.1"
                     /translation="MGEAVGLGLGLRVAVEVGRDVGGKLAVASVPPHVPAVGLEHVDG
                     TGAVGAAVGVLLPQPAIANPSTTASAALTIRLSRAHEP"
BASE COUNT          422 a          807 c          852 g          410 t
ORIGIN      
        1 atggagcccg aacacatctc ggcatacgca ctggccctgg attcgtatgc gcccagcccc
       61 gaccacgtac ccgtctcgcg cggtgcctcc gcctggcgtg cgcgagcgcg cggcgcacag
      121 gaccaggacc gtgccgcagc catgtacgag ctcgccgacg atgagtttgg ccgtgccggc
      181 atgggttggt acgagatctc gaactgggcg cgccccggtc gcgccagccg gcacaacaac
      241 gtgtattggc agagtgagtc gtgggcggct gtcggaccgg gcgcgcacgc cttcgacgga
      301 gtggccactc ggcggtggaa cgacgcgtcg ctggacgcct atctcagcgc tctggacaaa
      361 gggtggctgc caaccggtgg ctccgcagtg tcggatgccg cttcggtcgc cggcgagcgg
      421 accatgctcg cgttgcgaac cgcggcaggc gtcccccgcg acgcgagcaa catggccctg
      481 acctgggggc tggacaacga cctcctggaa gggatcggag agcgcgtccg attgacccgc
      541 cgcggccgat tactctcgaa tgagctcttc gtcaggctcg taccggaggc ggaagcagcc
      601 gcggcgtaga cgaattccgc agttgtcgtc cggggcgacc cgcgacgttc ggttgcctga
      661 gactggctta cgaggcgtgc tcccgggcga catcggccag gatctgcgca aggtcatgag
      721 gccgtgacca catcggccag tggctggtcg gcaggtcgac gtaggtgacg tcagtctgat
      781 cagccagacc ggctacgaag tcgtagccct tctcgacggc gtcgcggtat tcctcggccg
      841 tgtaggcagt gcagattacg accgtcggaa tcgccttacg agcgtcgttc gcgagctccg
      901 tgctaccgcg cagaacaccg cccggctgcg gcacggcacg ctcgcggaac gtggcaagct
      961 gctgttcgga aagtccgtcg aggttctcgt tcgcggccag atcttcgggc gacgcaagag
     1021 gtttttcgac gtcggcgaac tcggcgtcca tggcccctac accggcgccg gtatcgacgt
     1081 agaccatcgc cgcgatcctt tccggaaccc ggtcggacgt cgcgtagccg ggaaagccag
     1141 cgccgctgtg aacggcgagg acgaccggcc gctcggacgc ctcgacggcc tgtgatatcg
     1201 cggcgatgtg gtcttcgaag gtcacgctgg agcggtcggc atcggtcgac tcaaggccgg
     1261 gaagcgttag cgcagtggcc accacgcctt cgctggcgag gttaacgatg acttcgtccc
     1321 acgcccaggc gccgagccac cagccgggaa caaggatgat cggagcaggt tgtgtcatgc
     1381 cccgaggtta cggctagtcg aggatgaata ccgactcgag cactggcgcg gagacccgca
     1441 cagcgcgatc ccacttttcg acggagccgc cgaccaggat cgccagcacg ccgccgtcga
     1501 tgtcaagcag attgatctgc aggcgacgcg ccgaagcaat cgatagcggg ccggccgcca
     1561 cagtgagcac ctgatggaag gctggtgggt ccgtgtcgag cggatcggtc gactcgacgg
     1621 tcagccgaac ggctggccgg ccgttgatct ccacgtccgt cggtccgtcg ataaccaggt
     1681 tttccctagc tgcgatctgc tgcgcggcct cggcggctgt ttcggcgccg acgatgttgg
     1741 cgaactgcac cgcaacaacg tccggcgagc ccgggtcatc ctggatgtca aaaaagcccg
     1801 gcgtctgctg ctcggttgtc cagcctcggc cgacctggaa cccaatgccc ggttctacaa
     1861 gacggctgat gtagtaattc ggcgggagac gtgtgcgcgg cccgggcaaa gggggcggac
     1921 ccgtgcagcc cagcggcccg ccgtcccaga ccagcggcgg agccacggca ccgcatccgg
     1981 aagtgggcga agcggtcggg ctggggctcg gactacgagt tgcggtcgaa gtcggtcgtg
     2041 atgtcggcgg caagctggcg gtggcttccg tgccgccgca cgtgccggct gtcggtctcg
     2101 aacacgtgga tggcacaggg gccgttggcg cggcggtcgg tgtgttgctt ccgcagccgg
     2161 ccattgccaa cccgagcacg actgcgagcg ctgctctcac gatccgattg tcgcgtgcgc
     2221 atgagccatg agcgttgacc tcggatcacg tcaggggtaa gatgcgctaa ccgttagcac
     2281 tccggctgtg agagtgctaa cagcctcaag cgcaacccaa aggtaggtat atggcgcgcc
     2341 gcaaggaacg ccccgccggc ccgctcgacc cccgatctct acagatcctg cgggccgtca
     2401 tcgatgaata cgtcgccacc gctgagccgg tcggcagcca gaccctggtc gagcgctttc
     2461 gactgggcgt cagctcggcc acggttcgca a
//
LOCUS       DATLCO010000064         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9014072, whole genome shotgun sequence.
ACCESSION   DATLCO010000064 DATLCO010000000
VERSION     DATLCO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9014072"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VMZ33_01690"
     CDS             <1..839
                     /locus_tag="VMZ33_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HUR15972.1"
                     /translation="AYDSIAGDHRQALPGAAAVELGHNFSLVHDDIEDNDVERHHRAT
                     LWKVWGVAQAINTGDTLFTLSRMALHRLSDLGFSDGKVLALMRLYDETCLALCEGQFM
                     DIWSAEQNDRLSVDFYFDMIGRKTAALIAASVQSGALLATDDERIIDAYRGFGWSLGM
                     AFQLNDDLLGIWGDERATGKEPSDLAKHKKTLPVIYALERAEEPDRVRLREIISHADL
                     SLAEVAEARAILDRSGAGAYTRLRAHAERDEALLRLETAGVVNAHSLERLRQIIVSAI
                     SA"
     gene            complement(848..2167)
                     /locus_tag="VMZ33_01695"
     CDS             complement(848..2167)
                     /locus_tag="VMZ33_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HUR15973.1"
                     /translation="MEIFIIALLILLNAFFVIAEYSLIRVRRSRIEQLVEEGSRGAKR
                     VNRLISQPGRFLATIQIGVTFVGFLAAAFAGASLVDDLEGLMVGLPALQGQSRLIALL
                     VVTAVVALFTIVFGELVPRAIALAHAESLALVLAVPVELLGRVLAPLVWLLTTLTNAI
                     GRLFGIKETHQETVTAEELMILVERGSEQGVIEAEEQQMIGAVLELGDLRAHEVMVPR
                     IDIKALPIAATLQEIVNAIVSEGHSRIPMYDETIDNVIGILYAKDLIPYLANGEVPDI
                     SALLRSPLFVPESISVDDLLHQLQRRKVHIAIVLDEYGGTAGLITIEDLIEEIVGEIQ
                     DEYDVEEPMIVSISDDEARVDGRASVDDLVEHFGATLDDEDREQYDTVGGLVYHEIGG
                     VPEVGDTVEVDGLTLTVESTDGRRVGKVLVVRRKLDGELSAADAEVA"
     gene            complement(2176..>3094)
                     /locus_tag="VMZ33_01700"
     CDS             complement(2176..>3094)
                     /locus_tag="VMZ33_01700"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HUR15974.1"
                     /translation="KDASPAEREVRLINAGLYAFDLAWLRDAVTRLTPSAATGELYLT
                     QLVQLARGAGHSVVAVEQPDEADWSTELIGINDRVELAEAQLVLQNDIIERLMESGVT
                     IEDPDAVTIEATVIVEPDVTIERNVALRGRTRIGRDSLVRAGSQIFDAIIGERCLIWA
                     SVIESSSIGNDVRIGPFAHVRPGCEIGDAAEIGNFAELKNTRFGPRSKQHHFSYLGDA
                     DIADDVNIGAGTITANYDGKQKHRTTIGKGAFIGSDTILRAPIEIGAGAYTGAGSVVT
                     RDVPAGKLAVGVPARIREKLDRPTDGEGG"
BASE COUNT          544 a         1033 c          972 g          545 t
ORIGIN      
        1 tggcgtacga ctccatcgcc ggcgaccacc gacaggcact gcccggggcg gctgcggtgg
       61 agctcggcca caacttcagc ctggtccacg acgacatcga ggacaacgac gtcgaacgcc
      121 accaccgcgc caccctctgg aaggtatggg gtgtggccca ggcgataaac accggcgata
      181 cgcttttcac gctgtctcgg atggcgcttc accggctgag cgacctcggc ttctcagacg
      241 ggaaagtgct cgcgctgatg cgcctgtacg acgagacgtg cctggccttg tgtgaaggcc
      301 agttcatgga catctggagc gccgagcaga acgatcggct atccgtcgac ttctacttcg
      361 acatgatcgg ccgcaaaacc gcagcattga tcgctgcctc cgttcagtcg ggggcgctgc
      421 tggccacaga cgacgaacgg atcatcgacg cctaccgggg cttcggctgg tcgctgggca
      481 tggcctttca gctcaacgat gacctgctgg gcatctgggg cgacgagcgg gcgactggca
      541 aggagccgtc cgacctggcg aaacacaaga agaccttgcc ggttatctat gcgctcgagc
      601 gggccgagga acccgaccgc gtccggctgc gcgagatcat ctcgcacgcc gatctttccc
      661 ttgccgaggt cgcagaggct cgcgcgatcc tcgatcgctc cggggcaggc gcctacacgc
      721 gtctgcgcgc ccatgccgag cgcgatgagg cgctgctgcg actggagacg gccggcgtcg
      781 tcaacgccca ttccctcgag cggcttcgcc agatcatcgt ctcagccatc agcgcctgac
      841 cgttgaacta ggcgacctcg gcgtcggcgg ctgagagctc accgtcaagc ttccgtctca
      901 caacgagcac cttgcccacc cgccttccgt cggtcgactc aaccgtgagg gtgaggccgt
      961 ccacttccac ggtgtcgcct acctccggca ccccgccaat ctcgtggtag acgagccccc
     1021 caaccgtgtc gtactgctcg cgatcctcat cgtcgagcgt tgcgccgaaa tgctctacga
     1081 gatcgtcgac actcgcccgg ccgtcgacgc gcgcctcatc gtcggagatt gacacgatca
     1141 tcggctcttc aacgtcgtac tcgtcctgga tctcgccgac gatctcctcg atgaggtcct
     1201 caatcgtgat caggcctgcc gtaccgccgt actcgtcgag cactatcgcg atatggacct
     1261 tgcgccgttg aagctgatgc agcaagtcgt ctacggagat cgactcgggg acgaacagcg
     1321 gcgatcgaag caacgcgctg atgtcgggca cctcgccatt ggccaggtac gggatgaggt
     1381 ccttggcgta gaggatgccg atgacgttgt cgatcgtctc gtcgtacatg gggatgcgtg
     1441 agtggccctc cgacacgatg gcgttgacga tctcctggag cgtcgcggcg atgggcagcg
     1501 ccttgatgtc gatgcgcgga accatgacct catgtgcgcg gagatcgcca agctcgagga
     1561 ccgcgccgat catctgctgc tcctcagcct cgatcacgcc ctgctctgag ccgcgctcca
     1621 cgaggatcat cagctcctcg gccgtgaccg tctcctggtg tgtctccttt atgccgaaga
     1681 gccggccgat ggcattggtc agggtcgtga gcagccacac cagcggcgcc agcacgcgtc
     1741 cgagcaactc cacgggcacg gccaggacca gggccaacga ctctgcgtgg gccagtgcga
     1801 tggcccgcgg caccagctcg ccgaacacga tcgtgaacag ggccaccacg gcggtgacca
     1861 cgagcagggc gatgagccgc gactggcctt gaagggccgg caggccgacc atcagaccct
     1921 cgaggtcgtc gaccaggctt gctcccgcaa atgctgcggc aaggaagccc acgaaggtca
     1981 cgccaatctg gatcgtcgcc aggaaccgac cgggctggct gatcagccgg ttgacccgct
     2041 tcgcaccgcg actcccctcc tcgaccagct gctcgatgcg cgaccggcgg acgcggatga
     2101 gcgagtactc ggcgatcacg aagaatgcgt tgagcaggat cagcagggcg atgatgaaaa
     2161 tttccacggt cggcgttagc ccccttcgcc gtcggtcggt cggtcgagct tctcgcggat
     2221 gcgcgccggc acgccaaccg ccagcttgcc cgctggaacg tctcgcgtca cgacggagcc
     2281 ggcacccgtg taggcgcccg cccctatctc aataggcgcg cgcaggatcg tgtcggagcc
     2341 gatgaaagcg cctttgccga tcgtggtccg atgcttctgc ttgccgtcgt aattggcagt
     2401 gatcgtgccc gctccaatgt tcacgtcatc ggcgatgtcg gcgtcgccca ggtagctgaa
     2461 gtgatgctgc ttggaccgtg ggccaaagcg cgtgttcttc aactcggcga agttgccgat
     2521 ctcggcggcg tcaccgatct cacagcccgg tcgaacgtga gcgaaggggc cgatgcgaac
     2581 gtcgttgccg atggacgacg actcgatcac ggacgcccag atcaggcagc gctcgccgat
     2641 tatggcgtcg aagatctgac taccagcgcg gacgagcgag tcgcggccga tgcgggtgcg
     2701 accacgcagt gcgacgttgc gctcgatggt gacgtcgggc tccacaatca cggtggcctc
     2761 gatcgtcact gcgtcagggt cctcgatcgt cacgcccgac tccatcagtc gctcgatgat
     2821 gtcgttctga agcaccagct gcgcttcggc gagctcgacg cgatcgttta tgccaatcag
     2881 ctccgtcgac cagtccgctt catcgggctg ctcgacggcc acgacggagt ggccggcgcc
     2941 acgggccagt tgaacgagct gcgtcaggta cagctctccg gtagccgctg acggcgtcag
     3001 gcgagtgacc gcatcgcgca gccacgccag gtcgaacgcg tacaggccgg cattgatgag
     3061 cctcacctcg cgctcagcgg ggctcgcgtc cttc
//
LOCUS       DATLCO010000065         2209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6000382, whole genome shotgun sequence.
ACCESSION   DATLCO010000065 DATLCO010000000
VERSION     DATLCO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6000382"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VMZ33_01705"
     CDS             complement(<1..717)
                     /locus_tag="VMZ33_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HUR15975.1"
                     /translation="MTSRSSLSEASLVHFPDKPFAYGAGPSTLRVLVRAPSDVVRGGR
                     VAFNDRYAWAGTDYQPLKETAPLWRYAHDGTADYWAADLALHPPRIRYRFGLETQADG
                     LRWFGADGLRDEPRPRGAFEFAYLAEGDRTVSPDWARGAVFYHIFPDRFASGSGAPAR
                     RGRANWDDVPTSESFFGGDLDGITEHLDHIASLGVDALYLTPIFSAPSNHKDAPADYV
                     AVDPAVGGDEALRRLIGAVHE"
     gene            complement(728..1633)
                     /locus_tag="VMZ33_01710"
     CDS             complement(728..1633)
                     /locus_tag="VMZ33_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HUR15976.1"
                     /translation="MTTPARAVSASARSRSMGVRNRRRGTLILKVVIMVIAVIYALFP
                     IVYVISAALNPANTLVGLGLIPDHVTLDNFTRLFNSPNAPWPRWILNSVIVAGTTAVL
                     VTSLTALAAYAFSRLRFRFRRHALVALVVVQLFPNMLAFVALFLLLQSVGNVIPQLGL
                     NSLGGLILVYSGGALGFNTWLMKGYFDTLPFDLDESAMVDGATSFQAFWYIILPLVRP
                     ILAVIGILTFISAYSDFLLARVLLSSTENYTLAVGMTLFLREYNLEWGTFAAAALIGA
                     LPIVAVFLVLQRQLIGGLAQGGVKG"
     gene            complement(1630..>2209)
                     /locus_tag="VMZ33_01715"
     CDS             complement(1630..>2209)
                     /locus_tag="VMZ33_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUR15977.1"
                     /translation="TIPGFISALVWVGLLNPLYGPINGVIEDLVGSSPRWFQDGNLAK
                     VALLMINTWLGYPYMLLVSLGALQSIPSDMYEAAEIDGASPWQRFRTITFPLLLVALA
                     PLLIGAFAFNFNNFTLIELVTEGGPPTPGSQTPAGQTDILISYSYRLAFAGGRGQDYA
                     LAAAISIVIFLIVAAITLFNFRLSRRVENLMS"
BASE COUNT          443 a          663 c          780 g          323 t
ORIGIN      
        1 ctcgtggact gcgccgatca accggcggag cgcctcgtcg ccgccgaccg ccggatcgac
       61 cgcgacgtag tcggcggggg cgtctttgtg gttggacggc gcgctgaaga tcggagtcag
      121 gtacagcgca tcgacgccca gggaggcgat gtggtcgaga tgctcggtga tcccgtcgag
      181 gtcgccgccg aagaacgatt cggaggtcgg cacatcgtcc cagttcgccc gcccgcgccg
      241 cgcgggcgca cctgagccgc tagcgaatcg gtcgggaaag atgtggtaga aaaccgcacc
      301 ccgtgcccaa tcgggcgaga cggtcctgtc gccctccgcc aggtacgcga actcgaacgc
      361 accgcgtgga cggggctcgt cgcgcagtcc gtccgcgccg aaccagcgca ggccgtctgc
      421 ctgagtctcc aatccgaagc gataacggat acggggcggg tgcagtgcca ggtcggctgc
      481 ccagtaatcg gccgtgccgt cgtgcgcgta ccgccacagt ggtgccgttt ccttcagtgg
      541 ctggtagtcg gtgcccgccc aggcgtagcg gtcgttgaac gcgacacgtc caccgcgaac
      601 gacgtccgag ggcgcgcgca cgaggacacg caaggtcgaa ggaccggcgc cataggcgaa
      661 cggcttgtcc gggaagtgga cgagcgatgc ctcgctcagc gagctgcgag aagtcaggga
      721 gccggaccta gcccttcact ccgccctgtg ccaggccgcc gatgagctgc cgctggagga
      781 cgaggaacac ggcaacgatc gggagcgcgc cgatgagcgc cgccgccgcg aacgtgcccc
      841 attcgaggtt gtattcacgc aggaacagag tcatgccgac ggcgagcgtg tagttctccg
      901 tgctcgagag caggacgcga gcgagcagga agtcggagta ggcgctgatg aacgtcagga
      961 tgccgatgac cgcgagaatc ggccggacga gcggcaggat gatgtaccag aacgcctgga
     1021 acgaggttgc accgtcgacc atcgcggact cgtcgaggtc gaacggcaga gtgtcaaagt
     1081 agcccttcat cagccacgtg ttgaagccaa gcgcgccgcc tgagtagacc aggatcaagc
     1141 cgcccaagct gttcaggccg agctgcggga tcacgttgcc aactgactgg agcagcagga
     1201 acagggctac gaaggcgagc atgttgggga acagctggac gaccacgagt gcgaccaagg
     1261 cgtggcggcg gaagcggaag cggagccgtg agaacgcgta cgcggcgagg gccgtgaggc
     1321 tcgtcacgag cacggcggta gtgccggcga cgatgaccga gttgaggatc cagcgcggcc
     1381 acggagcgtt ggggctgttg aacagccgcg tgaagttatc cagcgtgacg tggtccggaa
     1441 tgagaccgag gccgaccagc gtgtttgccg ggttgagcgc cgcgctgatc acgtagacga
     1501 tggggaaaag cgcgtagatc acggcgatga ccatgatcac gaccttcagg atgagcgtgc
     1561 cacgccgacg attgcgtacg cccatcgagc gcgaccgagc tgatgccgag acggcgcgtg
     1621 cgggagtcgt catgacatga ggttctccac gcgccggctg agccggaagt tgaacaacgt
     1681 gatcgcggcc acgatcagga agatcacgat ggagattgca gccgccaacg cgtagtcctg
     1741 gccgcggccg ccggcaaacg cgaggcggta gctgtacgag atcaggatgt ccgtctggcc
     1801 tgcgggtgtc tgggagcccg gtgtcggtgg cccgccctct gtcaccagct cgatcagagt
     1861 gaagttgttg aagttgaacg cgaaggcgcc gatcaacagc ggtgccaacg cgacgagcag
     1921 cagcggaaag gtgatcgtcc ggaagcgctg ccatgggctc gcgccgtcga tctcagccgc
     1981 ctcatacatg tcagagggga tgctctgcag cgcgccgaga gacaccagca gcatgtatgg
     2041 gtagcccagc caggtgttga tcatcagtag cgccaccttc gccaggttgc cgtcctggaa
     2101 ccagcgcggc gacgagccga cgagatcctc gatgacaccg ttgatcggcc cgtataacgg
     2161 gttcagcaag ccgacccaga caagcgccga gatgaagccc ggaatcgtg
//
LOCUS       DATLCO010000066         3636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1567612, whole genome shotgun sequence.
ACCESSION   DATLCO010000066 DATLCO010000000
VERSION     DATLCO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567612"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VMZ33_01720"
     CDS             complement(<1..1143)
                     /locus_tag="VMZ33_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HUR15978.1"
                     /translation="MAPSNDLTRLHAHEMARGLRAGDFSARDLIDTHLARIEATDGPL
                     HAWVHLDAEEARRQADAADATIKTARADGQQALDNLPDLVGVPVALKDLVVTRGQPST
                     AGSRILEGFISPYDAHIAERLRSAGAVFPGKTNMDEFAMGSSTEHSAWGPTANPWDLE
                     RVPGGSSGGSAAAVAAYHTPLGVGTDTGGSIRQPAALTGTVGLKPTYGRVSRYGIVAF
                     ASSLDQIGPFGRDVRDTATLMAALAGRDERDSTSAPVEVPDYPSHLAGSNDEAAGFVR
                     GMRLGLPKQYFVDGMEPGVEARVREAVAALADAGAEIVDVDLPHTDYGLATYYIIAPA
                     EASANLARYDGIRYGHSIREEGRDYIADYLATRGTGFGAEVKRRIML"
     gene            complement(1159..1461)
                     /gene="gatC"
                     /locus_tag="VMZ33_01725"
     CDS             complement(1159..1461)
                     /gene="gatC"
                     /locus_tag="VMZ33_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HUR15979.1"
                     /translation="MATLSRKDVEHVAFLARLGLTEDELARLEGQLNHILDQYSVLAQ
                     LDTEGIAPTAQTIELENILRADEVRPSLKPEEILDGAPQRRGEYFVVPPILGEHGN"
     gene            complement(1534..>3636)
                     /gene="ligA"
                     /locus_tag="VMZ33_01730"
     CDS             complement(1534..>3636)
                     /gene="ligA"
                     /locus_tag="VMZ33_01730"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HUR15980.1"
                     /translation="TDPAARIAELRARIERANKLYYEQDAPEISDAEWDALFHELVQL
                     EEEYPALRTPDSPTQRVGGAPNVQLAEVRHSTPMLSLNNAFSYDDVRAFDARVRRLLG
                     MDDAAAARDLRYVCELKIDGLAVSVRYDAGRLVQAATRGDGSTGEDVTNNIRTIESVP
                     KRLRERVSVVARGEVFMPKAEFARINAEREELGLPLYANPRNSGAGSLRQIDPQVTAS
                     RRLATWFYVLIEGEATETGSSETVDTQSAALDRLDKLGLPAEPNRQPGLSIDDVVAHI
                     ERWQDVRHDLAYETDGMVIKVERFDLQRRLGMASHAPRWAIAYKYPPEQVETVVEDIV
                     AYVGRTGTLTPVAHLTPVKVAGSTVGRATLHNLDEVRRKDVRVGDHVVLHKAGDVIPE
                     IVRAITDRRSADVPEWDMPTACPVCSTPVVRDPDTVRHYCPNVACPARVGQEYEHFVG
                     RGSMDIEGAGWKVLAQLLEKGLVRRRGDFFRLTVGDLESLDRFARKSAENLYASIQRA
                     RVRPLFRIISGLGIPQVGGQTAIDLSTWIARRWPPADDEPMGGKEGWFQRIAASLRGV
                     SAAEFEEISGVGPTVAASLERWFADEATASALDDLVDAGVEPERPDPAVLADATVAGP
                     LAGKTVVVTGTLDGFDRQAAEEAIRAAGGKAGGSVSKKTDFLVAGDNAGTKLAKAEEL
                     GVPVLDEAAFRHLLAGNG"
BASE COUNT          545 a         1216 c         1265 g          610 t
ORIGIN      
        1 gagcatgatc cggcgcttca cctcggcacc gaagccggtt ccgcgggtgg cgaggtagtc
       61 ggcgatgtag tcgcggccct cctcgcggat cgagtggcca tagcgaatgc cgtcgtagcg
      121 ggcgaggttc gccgaggcct ccgccggggc gatgatgtag tacgtcgcga ggccgtagtc
      181 ggtgtggggc aggtcgacgt cgacgatctc cgcgccggcg tcggcgagcg cggccacagc
      241 ctcgcggacg cgggcctcga cgcccggctc catgccgtcg acgaagtact gcttgggcag
      301 cccgaggcgc atgccgcgaa cgaagccggc cgcctcgtcg ttgctgcccg cgaggtggct
      361 cggatagtcg gggacctcga ccggggctga ggttgagtcg cgctcatcgc gtccggcaag
      421 ggcggccatt aacgtcgccg tgtcgcgcac gtcgcggccg aagggcccaa tctggtcgag
      481 cgagctggcg aaggcaacga tgccgtaacg gctgacgcgg ccgtatgtgg gcttgagacc
      541 gaccgtgccc gtaagcgcgg ccggttggcg gattgagccg ccggtgtcgg tcccgacgcc
      601 gagtggtgtg tggtaagcgg ccacggccgc cgctgagcca ccgctgctgc cgccggggac
      661 gcgctcgagg tcccatgggt tggccgtcgg gccccaggcc gagtgctccg tcgacgagcc
      721 catggcgaac tcgtccatgt tcgtcttccc cgggaagacg gctccggccg agcgcaagcg
      781 ctccgcaatg tgcgcgtcgt aagggctgat gaagccttcg aggatgcggc tgccggccgt
      841 cgatggttgg ccgcgggtca cgaccagatc cttgagcgcc accggaacgc cgaccagatc
      901 tggcaggttg tcgagagctt gctggccatc ggcgcgggcc gtcttgatgg tcgcatccgc
      961 ggcgtcagcc tgacggcgcg cctcttcggc gtcgaggtgc acccatgcgt ggagtggccc
     1021 gtcagtggcc tcgatccggg cgaggtgggt atcgatcaaa tcgcgcgccg agaagtcgcc
     1081 tgcgcgcaga ccgcgcgcca tctcgtgggc gtggaggcgc gtcaggtcgt tggagggggc
     1141 catgctcaga gccgtggtct agttgccgtg ctcgccgagg atcggcggca ccacgaagta
     1201 ctcgccgcgc cgctgcggcg cgccgtcgag aatctcttcg ggcttcaggc tgggccgcac
     1261 ctcgtcggct cggaggatgt tctcgagctc gatggtctgc gcggtgggtg cgatgccctc
     1321 ggtgtcaagc tgcgccagca ccgagtactg atcgaggatg tggttgagct gaccttcgag
     1381 gcgcgcgagc tcgtcctcag tcagtccgag gcgagccagg aatgcgacgt gttcgacgtc
     1441 cttgcgagag agcgtggcca cgcccgaagg atagccgcgg acgcttccgg cctggagcac
     1501 gcacatggaa ctggcgccaa atgtgctgca gtgtcagccg ttgcctgcga gaagatgccg
     1561 gaaagccgcc tcgtccaaca cagggacacc gagctcctct gctttggcca gctttgtgcc
     1621 ggcgttgtcg ccggcaacga ggaagtcggt cttcttgctg accgacccgc cggccttgcc
     1681 acccgcggcg cggatcgcct cctctgcagc ctgtcggtcg aagccatcca gcgtccctgt
     1741 caccacgacg gtcttgccgg cgagtggccc cgccacagtg gcgtcggcta ggactgcagg
     1801 gtccggccgc tcgggctcga cgccggcatc gaccagatcg tcgagcgcgg acgcggtcgc
     1861 ctcgtcggcg aaccagcgct cgaggctcgc ggccacggtc ggacccacac ccgagatctc
     1921 ctcgaactct gccgcggaca ctccccgaag gctggccgcg atcctctgga accagccctc
     1981 cttgccgccc atcggctcgt catcggcagg tggccatcga cgcgcgatcc aggtcgacag
     2041 gtcgatcgcg gtctgcccgc caacctgcgg gatgcccagg ccactgatga tccgaaacag
     2101 cggccgcacg cgcgcacgct ggatgctggc gtagagattt tcggcgctct tgcgcgcgaa
     2161 acgatcaagg ctctccaggt cgccgacggt caggcgaaag aagtccccac ggcgcctcac
     2221 caatcccttc tcgaggagtt gggccagcac cttccagccg gcgccttcga tgtccatgga
     2281 gccacggccg acgaaatgct cgtactcctg cccaacgcga gccgggcacg cgacgttggg
     2341 gcagtaatgg cggacagtgt ccgggtcgcg cacgaccggc gtggaacaca cggggcacgc
     2401 ggtgggcatg tcccactccg gcacatccgc gctacgcctg tccgtaatgg cgcggacgat
     2461 ctcgggaatt acgtcgccag ctttgtgcag cacgacgtgg tcaccgacgc ggacatcctt
     2521 gcggcggacc tcatcgaggt tgtgaagagt tgcccggccg acagttgaac cggcgacctt
     2581 gaccggtgtc aggtgcgcga cgggcgtgag cgtgccggtg cgcccgacgt aggcgacgat
     2641 gtcctccacg acggtctcca cctgctcggg cgggtacttg taggcgatcg cccaacgcgg
     2701 cgcgtgcgat gccataccca gccggcgctg gaggtcgaag cgctcgacct tgatgaccat
     2761 gccgtcggtc tcgtaggcca ggtcgtgtcg gacgtcctgc cagcgctcga tgtgggcgac
     2821 gacgtcgtcg atcgaaagcc ccggctggcg attgggctcg gccggcagac caagcttgtc
     2881 gagcctgtcc agggcagccg actgggtgtc gaccgtttcc gacgagcccg tctccgtggc
     2941 ctcaccctcg atcaacacgt agaaccatgt cgccaggcgg cggctggcag tgacctgcgg
     3001 gtcgatctgg cggagcgagc cggcaccgct gttgcgcgga ttcgcgtaga gcggtagccc
     3061 cagctcctcg cgctcagcgt tgatgcgcgc gaactccgcc ttgggcatga agacctcgcc
     3121 gcgcgcgacg accgacacac gttccctgag cctcttcggc actgattcga tggtgcggat
     3181 gttgttcgtg acgtcctcgc ccgtcgagcc gtcgccgcgc gtcgcagcct gtaccaggcg
     3241 ccccgcgtcg tagcggacgc ttaccgccag gccatcgatc ttgagctcgc agacgtatcg
     3301 caggtccctc gcagcggctg cgtcgtccat ccccaacagg cgccgcaccc gggcgtcgaa
     3361 agctcgtacg tcgtcgtacg agaaagcgtt gttgagcgac agcatcggcg tggagtggcg
     3421 cacctcggcg agctggacgt tgggcgctcc accgacgcgc tgcgtaggcg aatccggcgt
     3481 ccgcagcgcc gggtactcct cctccagctg cacgagctcg tggaaaagtg cgtcccactc
     3541 agcgtcgctg atctcgggcg cgtcctgttc gtagtacagc ttgttggctc gctcgatccg
     3601 tgcgcgcaac tcggcgatgc gggcggcggg gtcagt
//
LOCUS       DATLCO010000067         4407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_822182, whole genome shotgun sequence.
ACCESSION   DATLCO010000067 DATLCO010000000
VERSION     DATLCO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822182"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VMZ33_01735"
     CDS             complement(<1..482)
                     /locus_tag="VMZ33_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR15981.1"
                     /translation="MNFPETVTEPQIHARELTKKFGDFVAVDGIAFDVAPGEAFGFLG
                     PNGAGKTSTMRMIACVSPRSGGELTVLGHDPEVNGPRIRARLGVVPQLDTLDTELSLM
                     ENLIIYGRYFDLSYAEARSRAKELLEFVQLNDRADSLVEPLSGGMKRRLTIARSLINE
                     P"
     gene            591..1100
                     /locus_tag="VMZ33_01740"
     CDS             591..1100
                     /locus_tag="VMZ33_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUR15982.1"
                     /translation="MVEVTWIPASVVELIEAALVTELSVVRGDGRPITYPLIPLWDGE
                     KVLMTSSILFSRKLEHVKENAHVSLSFSDPVALGGLSGRATIQGEARVIDGDPHTDWE
                     AVLPLWSAKEPVILQFLKARVAFPLFFERSVIEVVPRRVLFWPDGDTSSPPQVFFAPA
                     VGESSGKAA"
     gene            1102..2505
                     /locus_tag="VMZ33_01745"
     CDS             1102..2505
                     /locus_tag="VMZ33_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HUR15983.1"
                     /translation="MDVRSGVSMFDPAEGLRRLTDFPFGLLTWVEASGYPMSAAVDFS
                     VEPEADVVSFVGPAGFDLPLDSEFSLTGSHIRPQPGVGYDERRHVTIWGRPTRRDDGR
                     LALAPTAAWGWDESSIPFPEYAERQVGQSRRYLARVSSEVGRHVTPKLSLGWLTLRAT
                     RLPFLSATFIPVILGIAIAAVHGSFDLFAAVLTLVGAAAVHLALNVANDVFDARLGAD
                     ENNVNPTQFSGGSRVIQYGLVSLRRMGAISASLYAVAIGVGLILLLTRFSTALLVIGV
                     LGVLLSFFYTAPPLKLVYRGLGELTTAIGFGPLMLVGAYVVQTVGVLRPEPFVASIPI
                     ALLVMLILYLNEVPDRPGDERAGKRTLPVRLPQGTVINGYLVAAIAAFAVIALGVLGG
                     LLPIPALAALLPLPLVGRVRDGLLRYYDQPYGLMAFLAVNIKVHLYVGVLLIAAYLSV
                     LVITSVAPSVPLFIRWN"
     gene            2527..3003
                     /locus_tag="VMZ33_01750"
     CDS             2527..3003
                     /locus_tag="VMZ33_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HUR15984.1"
                     /translation="MRVSAKADYAVRAAAELAAAAGTRPLKREQISQAQNIPSKFLET
                     IMLELKHAGIVKSTRGADGGYALARPATDISVADVIRAVDGPLANVRGERVETVEYEG
                     AARALRDVWVAVRASLRRVLETTSLQDLVEDHLPESVRELTADPEAWISLNRRRRS"
     gene            complement(3004..>4407)
                     /locus_tag="VMZ33_01755"
     CDS             complement(3004..>4407)
                     /locus_tag="VMZ33_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HUR15985.1"
                     /translation="WQEAPSSAPYPDPVDFQDVGVNPFVDPTRDPFSTFAMDVDTASY
                     AVTRRFLEDGNMPDRDSVRLEEFVNSFNYDYRSPQDSAFAIHVEGAPAPFVDSRSILL
                     RIGIQARELNEFSRKPVALTFVIDTSGSMDMENRLELVKAALGLLVDRLSPDDSVAIV
                     RFGDDAEVVLPPTSAAEPEQIRRAIADLHTGGSTNVQAGLELGYDMAALGHRSGATDR
                     VVLASDGVANVGLTNADEILRRIDREVDAGVELVSVGVGMGNFNDVLLEQLADRGNGF
                     YAYINDRLEAERLFGEELIGTLQTVARDARVQVEFHPDAVLRYRLLGYENRRMADGDF
                     ENPYVDAGEVGAGHSVTALYEIEPSFSRDAFLGSVRVRWVDPDTNAEQEEAVDIDLFS
                     ISDDYRSTSPGFQLAATVGGFAEILRESPYVNGYTLDDIAREAASLSALFENDLDVSV
                     FRELTEIAARLDRGFDF"
BASE COUNT          718 a         1417 c         1434 g          838 t
ORIGIN      
        1 ggctcgttga tgaggctccg ggcaatcgtc aaccggcgct tcataccgcc cgacaatggc
       61 tcgacgaggc tgtcggcacg gtcgttgagc tggacgaact cgagcagctc cttcgcgcgc
      121 gatcgcgcct cggcgtacga caggtcgaag taccggccgt agatgatgag gttctccatc
      181 agcgacagct cggtgtcgag agtgtcgagc tgaggcacca cgccgaggcg ggcgcggatg
      241 cgcggcccat tcacctccgg gtcgtgcccc aggaccgtca actcgccgcc agaacgtggc
      301 gagacacacg cgatcatgcg catcgtcgat gtcttgccgg cgccgttggg accaagaaag
      361 ccgaaggcct cgccgggagc cacgtcgaat gcgatgccgt ccacggcgac gaagtccccg
      421 aacttcttgg tcagctcgcg ggcatgaatc tgcggctcgg tgacggtttc cgggaagttc
      481 atcgatggcg gatggtagcg cagctacccg ttctcgcccg ctgtgcggcg cgcgaaacgt
      541 gttaccattc agtcctgagt tattcccgag caattcgggt cgaatggagc gtggtcgaag
      601 tgacctggat acctgcttcg gttgtcgagt tgatcgaggc cgcgttagtc acggagctgt
      661 ctgtcgtgcg tggcgatgga cgtccgatca cctatccgtt gatccccctg tgggatggcg
      721 agaaggtgct catgacctcc tcgatcctgt tttcacgcaa gctcgagcac gtgaaggaga
      781 acgcgcatgt gtcgctgtcg ttctccgatc ccgtcgcgtt gggcggtttg agcgggcgcg
      841 cgacgatcca gggcgaggcg cgtgtgatcg acggcgatcc gcataccgac tgggaggccg
      901 tcctcccact gtggtcggca aaagagccgg tcatcctgca gtttctcaag gcgcgcgtgg
      961 ccttcccgct gtttttcgag cggtcggtca tcgaggtcgt gccacggcgc gtcctgttct
     1021 ggcctgacgg cgacacgtcc tccccgccgc aggtcttctt cgcgccagcg gtgggcgagt
     1081 cctcgggaaa ggcggcctga catggacgtt cgttctggcg tctcgatgtt tgacccggct
     1141 gaaggcctgc ggcgcctgac cgatttcccc ttcggcctcc tgacctgggt cgaagcgagc
     1201 ggatatccga tgagcgcggc ggtcgatttc tcagtcgagc cggaggccga cgtcgtcagc
     1261 ttcgtcggac ccgcgggctt tgatctgccg ctcgattcag agttctccct gactggcagc
     1321 cacattcggc cgcagcccgg cgtcggctat gacgagcggc gacacgtcac gatctggggt
     1381 aggccgacgc gccgcgatga cggccggctg gcgctggctc cgaccgcagc gtggggctgg
     1441 gacgagtcat cgatcccctt ccctgaatac gcagagcgcc aggtcggcca gtcgcgtcga
     1501 tatttggcgc gcgtttcgtc agaggttggc cggcacgtga cacccaagct gtcactcggc
     1561 tggctgacgc tccgtgcaac ccgcttgccg ttcctctcgg caaccttcat accggtgatc
     1621 ctgggcatcg ccatcgcggc ggtccacggt tcgttcgatc tcttcgcggc ggtgctgacc
     1681 ctggtcgggg ccgcagccgt gcacctggcg ctcaacgttg ccaacgacgt gtttgacgct
     1741 cgcctcggcg cagacgagaa caacgtaaat cccacgcagt tcagtggtgg gtcgcgcgtc
     1801 atccaatacg gcctcgtctc gctccgacgg atgggcgcga tctcggcaag cctctatgcg
     1861 gtcgccatcg gcgttgggct catcctgctg ctgacacgct tctcaacggc gctgctagtg
     1921 atcggcgttc tcggcgtcct gctcagcttc ttctacaccg cgccaccgct caagctcgtc
     1981 tatcgcgggc tgggcgagct gaccacggct atcggcttcg gtccactgat gctcgtaggc
     2041 gcttacgtcg tgcagaccgt aggcgtgctt cgaccggaac cgttcgtagc gtcgatcccc
     2101 atcgcgctcc tggtgatgct gatcctgtat ctcaacgagg tgcccgaccg gcccggcgac
     2161 gagcgtgccg gcaagcggac gctgcccgtt cggctaccgc agggcacggt catcaacggt
     2221 tacctcgtcg cggcgatcgc cgcgttcgcg gtaatagccc tcggggtgct cggcggcctg
     2281 ctgccaatcc cggcactcgc ggccctgttg ccgttgccgt tggttggccg cgttcgggac
     2341 ggcctgcttc gctattacga ccagccctac gggctgatgg cgttcctcgc ggtgaacatc
     2401 aaggtccatc tctacgtagg cgtgctgctc atcgcggcat acctgtcggt gctcgtgatc
     2461 acctcagttg cgccctcggt gccgctattt atccgctgga actgactaat cccacacaat
     2521 tactaggtga gggtttccgc caaggctgat tacgccgtcc gcgctgccgc cgagcttgcc
     2581 gctgccgccg gcacgcgtcc cctgaaacgc gagcagatct cgcaggctca gaacattccg
     2641 agcaagttcc tcgagacgat catgctcgag ctgaaacacg ccggcatcgt gaagagcacc
     2701 cgcggtgctg acggtggcta cgcattggct cgccccgcga cagatatcag tgtggccgac
     2761 gtgattcgcg ccgtggatgg gcctctcgcc aacgtgcgcg gcgagcgcgt ggaaacggtt
     2821 gagtacgagg gtgccgcacg agcgctgcgc gacgtgtggg ttgcggtcag ggccagtctc
     2881 cgccgagtcc tggagacgac cagcctccag gatctcgttg aggaccacct gcctgagagc
     2941 gtccgtgagc tgaccgccga tccagaggca tggatctccc tgaatcggcg gcgtcgctct
     3001 tagctagaag tcgaaccccc ggtccaatcg agcggcgatc tctgtcaact cgcggaacac
     3061 cgacacgtcc aggtcgttct caaacagcgc cgacagcgaa gcggcctcgc gggcgatgtc
     3121 gtcaagtgtg tagccgttga catacgggct ctcgcgcagg atctcggcaa agccgccgac
     3181 cgtcgccgcc agctggaagc cggggctggt cgatcggtag tcgtccgaga tcgagaacag
     3241 gtcgatgtcg accgcctctt cctgctcggc attcgtgtcg ggatcaaccc atcgaactcg
     3301 gacggaaccc aggaacgcat cgcgcgagaa agacggctcg atctcgtaca gcgccgtgac
     3361 ggagtgtccg gcgccgacct cgccggcatc cacgtaggga ttctcgaagt ctccgtcggc
     3421 catgcgtcgg ttctcgtagc caagaagccg atagcgcagc acggcgtcgg ggtggaactc
     3481 gacctgcacg cgggcgtcgc gcgcaacggt ctggagcgtg ccgatcagct cctcgccgaa
     3541 aaggcgttcg gcctccaggc ggtcgttgat ataggcgtag aagccattgc cgcgatcggc
     3601 cagctgctcg agcaatacgt cgttgaagtt gcccatccca acaccgaccg acacgagctc
     3661 aacgccggcg tcgacctcgc gatcgatccg ccgcaggatc tcatcggcgt tggtgaggcc
     3721 cacgttcgcc accccgtcgg acgcgagcac gacacggtcg gtggcgccgc ttcgatgccc
     3781 caacgcggcc atgtcgtagc cgagctcgag gccggcctgg acattggtcg aaccaccggt
     3841 gtgcaggtcg gcgatcgcac ggcggatctg ctctggttcc gcggcagagg tcggcgggag
     3901 cacgacttcg gcgtcgtcgc cgaagcggac gatggcaacg gagtcgtcgg ggctgaggcg
     3961 atcgaccagc agcccgagtg ccgccttgac cagctccagc cggttctcca tgtccatgga
     4021 gcctgacgtg tcgatcacga aggtcagggc cacgggtttg cgcgagaact cgttcagctc
     4081 acgcgcctgg atgccgatgc ggagcaggat tgagcggctg tcgacgaatg gtgccggtgc
     4141 cccctccaca tggatggcaa aggcgctgtc ctggggcgac cggtagtcgt aattgaagct
     4201 gttcacgaat tcttcgaggc gaacgctgtc gcggtcaggc atgttcccgt cctcgaggaa
     4261 ccgtcgcgtc acggcgtacg aagccgtgtc gacgtccatc gcaaaggttg agaacgggtc
     4321 gcgggtcggg tcaacgaacg ggttgacacc cacgtcctgg aagtcgacgg gatctggata
     4381 cggggcactg ctcggggcct cctgcca
//
LOCUS       DATLCO010000068         2189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6001362, whole genome shotgun sequence.
ACCESSION   DATLCO010000068 DATLCO010000000
VERSION     DATLCO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6001362"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..>2189
                     /locus_tag="VMZ33_01760"
     CDS             86..>2189
                     /locus_tag="VMZ33_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15986.1"
                     /translation="MPNSGFESDIGTFTAWALDLAERGSGSFYASAGFADYPPGYLYV
                     LWLIGGVGHMLADLTGGDAVAITTTLIKLPPMLADIAVGIALYWIARRWRAPRLDAHR
                     IGLTAAALYVFNPVTWYDSAIWGQTDAFGALVLVLTVAALVRGNSEGATVLSVLAAVI
                     KPQFGVVLIPLVGIVLLRRHLLKPGTNPRNRILAPPAAARWLAKEEGPLRLVSSAVLG
                     LIVMLVVITPFSLSIPGFVEQMAKTAGGYQFLSVNGYNPWAMIGSDGNQGIAWGGAWS
                     WNPDTVPLLGPVPGVMIGAVLLIVGFAIGLARVGWRDDRRSILLVGLFLALCFFILPT
                     RVHERYMFPIFGLLPLLAAVDRRWLWATVAMSTGAFINLHGVLTTDIYASPNLEHLPL
                     GDFARSTPGVLLSIALNVGGFAYVAWMMRRSAADERDPYASLARPRQIVPSAPGEPVA
                     APVPALPVSVSPAAMPAHGSRSLSAGAIAAAAVAGRAGGAMDQAALSTSSIVASDSEP
                     DADIDEPPQPPILQNLRNAWSRLVGSASTRRDRSGMLANELPSSRLDRKDLGLIALVF
                     LSTLLLRTYRLEVPYSMHFDEVYHARTAVEFLQNWRYDMPHDIYEFTHPHLAKYGMAL
                     GIMALGDNKVTSTRELGINVRDAVIEPRWNPDDNPGERDGDRLYVAGDNEVGVFELSN
                     KQQIATIPGTYEAIAVDEE"
BASE COUNT          333 a          743 c          700 g          413 t
ORIGIN      
        1 actgagagcg agtacggccc tatccggact cggaatactc ctgctcgtag cggtactcct
       61 tcgcctaact attgcatacg tccttttgcc gaactccggc ttcgagtcgg acatcggcac
      121 gttcaccgcg tgggcgctag acctcgcgga gcgcggttca ggcagtttct acgcttcggc
      181 ggggtttgcc gactacccac ctgggtacct atatgtactg tggctgatcg ggggagtggg
      241 ccacatgctg gccgacttga ccggcggcga cgccgtagcg atcaccacca ctctcatcaa
      301 gctgccaccg atgctggccg acatcgccgt tggcatcgcg ctctactgga ttgcccgacg
      361 ttggcgcgcc ccgcgactcg atgcccatcg aatcggcctg accgcggcgg cgctgtacgt
      421 cttcaatccg gtcacctggt acgactcggc gatctggggc cagacggatg cgttcggcgc
      481 gctggtcctg gtgctcaccg tggcggccct ggttcggggt aactccgaag gcgccaccgt
      541 cctgagcgtc cttgcagcgg tgatcaaacc gcagttcggc gtggtgctga ttcccctcgt
      601 cgggatagtg ctgcttcgtc gccacctgct gaagccgggc accaatcctc gaaatcggat
      661 cctggctccg cctgccgccg cacgttggct ggccaaggag gagggaccgc tgcggctggt
      721 gtcgtctgcc gtgctcgggc tcatcgtcat gctcgtcgtg atcacgccct tctcgctgag
      781 cattccgggt tttgtcgagc agatggccaa gacggccggc ggctaccaat tcctgagcgt
      841 caacggctac aacccatggg cgatgatcgg ctccgatggc aaccagggga tcgcctgggg
      901 cggcgcgtgg tcgtggaacc ccgacacggt tccgctgctc ggaccggtgc cgggcgtgat
      961 gatcggcgcc gttctgctga tcgtgggctt cgccatcggc ctggccaggg tcggctggcg
     1021 tgatgatcgc cgttccatcc tgctggtcgg gctattcctg gcgctgtgct tcttcatcct
     1081 gcccacgcgc gtccacgagc gctacatgtt cccgatcttc gggctgctgc cgctgcttgc
     1141 ggctgtcgac cggcgctggc tgtgggcgac ggtggccatg tcgaccgggg ccttcatcaa
     1201 cctccacggc gtcctgacga ccgacatata cgcgtcaccc aacctcgagc atctgccgct
     1261 gggcgacttc gcgcgctcga ctccgggcgt cctcctgagc atcgctctca acgttggcgg
     1321 cttcgcgtac gtggcctgga tgatgcgccg cagcgcggcc gatgagcgcg acccgtacgc
     1381 atcccttgcg cgccctcgcc aaatcgtccc gtcggcaccg ggcgaacccg tcgccgcgcc
     1441 ggtacctgcc ctgcccgttt cggtgtcgcc tgcagcaatg cctgcgcacg gctctcggtc
     1501 cctttcggcc ggtgccattg cggctgctgc ggtagcgggt cgcgcaggcg gcgcgatgga
     1561 tcaggcggcc ctctcgacct cctcgatcgt ggcgagcgac agcgaacccg acgcggacat
     1621 cgatgagccg ccccagccgc cgatcctcca gaacttacgg aacgcctgga gccgcctggt
     1681 cggctcggcg tccacacggc gcgaccggag cgggatgctc gccaatgagc tgccgtcgtc
     1741 gaggcttgac cgcaaggatc tgggcctcat cgcgctggtg ttcctcagca cgctgttgct
     1801 gcggacgtac cggctggaag tgccgtattc gatgcacttc gacgaggttt accacgcccg
     1861 aacggcggtc gagttcctcc agaactggcg ctacgacatg ccgcacgaca tctacgagtt
     1921 cacccacccg cacttggcca agtacgggat ggccctcggc atcatggcgc tcggcgacaa
     1981 caaggtcacg agcacgcgcg agctgggaat caatgtccgc gacgcggtca tcgagccgcg
     2041 ctggaatcca gacgacaacc ccggtgagcg cgacggcgac aggctctacg tagcgggcga
     2101 caacgaggtg ggcgtcttcg agctgagcaa caaacagcag atcgccacca ttccgggcac
     2161 gtacgaggcg atcgccgtcg atgaggaag
//
LOCUS       DATLCO010000069         2189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6001391, whole genome shotgun sequence.
ACCESSION   DATLCO010000069 DATLCO010000000
VERSION     DATLCO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6001391"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..>2189
                     /locus_tag="VMZ33_01765"
     CDS             86..>2189
                     /locus_tag="VMZ33_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15987.1"
                     /translation="MPNSGFESDIGTFTAWALDLAERGSGSFYASAGFADYPPGYLYV
                     LWLIGGVGHMLADLTGGDAAAITTTLIKLPPMLADIAVGIALYWIARRWRAPRLDAHR
                     IGLTAAALYVFNPVTWYDSAIWGQTDAFGALVMVLTVAALVRGNSEGATVLSVLAAVI
                     KPQFGVVLIPLVGIVLLRRHLLKPGTNPRNQILAPAGVARWLAREEGPVRLVTSAVIG
                     LVVMLVVITPFSLSIPGFVEQMAKTAGGYQFLSVNGYNPWALIGSDGNQGIAWGGAWS
                     WNPDTIPLLGPIPGVMIGAVLLIVGFVIGLTRVGWRDDRRSVLLVGLFLALCFFILPT
                     RVHERYMFPIFGLLPLLAAVERRWLWATVAMSIGAFINLHGVLTTEIYASPNLEHLPL
                     GDFARSTPGVLLSIALNVGGFAYVAWMMRRSAADERDPYASLARPRQVALAAPDAPVA
                     APVPALPVSVSPAAMPAHASRSLSAGAIAAAAVAGRAGGAVSQAALATSSIVASDSEP
                     DADIDEPPQAPILQGVRDAWGRLVGSTSIRRDRSGMLASELPSSRLDRKDLGLIALVF
                     LSTLVLRTYRLEVPYSMHFDEVYHARTAVEFLQNWRYDMAHDIYEFTHPHLAKYGMAL
                     GIMALGDNKVTSTRELGVNVRDAVIEPRWNSDDNPGERDGDRLYVAGDNEVGVFELSN
                     KQQIATIPGTYEAIAVDEE"
BASE COUNT          335 a          741 c          700 g          413 t
ORIGIN      
        1 actgagagcg agtacggccc tatccggact cggaatactc ctgctcgtag cggtactcct
       61 tcgcctaact attgcatacg tccttttgcc gaactccggc ttcgagtcgg acatcggcac
      121 gttcaccgcg tgggcgctag atctcgcgga gcgcggttca ggcagtttct acgcttcggc
      181 ggggtttgcc gactacccac ctgggtacct atatgtactg tggctgatcg ggggagtggg
      241 ccacatgctg gccgacttga ccggcggcga cgccgcagcg atcaccacca ccctcatcaa
      301 gctgccaccg atgctggccg acatcgccgt tggcatcgcg ctctactgga ttgcccgacg
      361 ttggcgcgcc ccgcgactcg atgcccatcg aatcggcctg accgcggccg cgctgtacgt
      421 cttcaatccg gtcacctggt acgactcggc gatctggggc cagacggatg cgttcggcgc
      481 gctggtcatg gtgctcaccg tggcggccct ggtccggggt aactccgaag gcgccaccgt
      541 cctcagcgtt ctcgcagcgg tgatcaaacc gcagttcggc gtagtgctga ttccactcgt
      601 cggcatcgtg cttctccgac ggcacctcct gaaacccggc actaacccgc gaaaccagat
      661 cctcgcgccc gctggcgtcg cacggtggct ggccagggag gaggggcccg tgcgactcgt
      721 gacgtcggcc gtgatcggcc ttgtcgtcat gctggtcgtc atcacaccgt tctcgctgag
      781 catcccgggc ttcgtcgagc aaatggccaa gacggccggc ggctaccagt tcctgagcgt
      841 gaatggctac aacccctggg cgctgatcgg ctcggacggc aaccagggca tcgcctgggg
      901 cggcgcgtgg tcgtggaacc ccgacaccat tccgctgctc ggcccgattc caggcgtgat
      961 gatcggggcg gttcttttga tcgttggctt cgtcatcggc ttgacacggg tcggctggcg
     1021 tgacgaccgc cgctcggtcc tgctcgtcgg cctgttcctc gccctgtgct tcttcatttt
     1081 gccgacgcgc gtccacgagc gctacatgtt cccgatcttc gggctgctgc cactgcttgc
     1141 ggcggtggag aggcggtggc tgtgggcgac tgtggccatg tcgatcgggg ccttcatcaa
     1201 cctgcacggc gtcctgacga ccgaaatcta tgcgtcaccc aacctcgaac acttgccgct
     1261 gggcgacttc gcgcgctcga ctccgggcgt cctcctgagc attgcgctca acgttggtgg
     1321 cttcgcgtac gtggcctgga tgatgcgccg cagcgcggcc gatgagcgcg acccgtacgc
     1381 atcccttgcg cgccctcgcc aagtcgccct ggcggccccg gacgcacccg tcgccgcgcc
     1441 ggtacctgcc ctacccgttt cggtgtcgcc tgcagcaatg cctgcgcacg cctctcggtc
     1501 cctttcggcc ggtgccattg cggctgctgc tgtagcgggt cgcgcaggcg gcgcggtcag
     1561 tcaggcggcc ctcgcgacct cctcgatcgt ggcgagcgac agcgaacccg acgcggacat
     1621 cgatgagccg ccccaagcgc cgatcctcca gggggtccgg gatgcttggg gccgtctggt
     1681 cggctcgacc tccatacggc gcgaccggag cggaatgctc gccagtgagc tgccgtcgtc
     1741 gaggctcgac cgcaaggatt tgggcctcat cgcgctggtg ttcctcagca cgttggtgct
     1801 gcggacgtac cggctggaag tgccgtattc gatgcacttc gacgaggttt accacgcccg
     1861 aacggcggtc gagttcctcc agaactggcg ctacgacatg gcgcacgaca tctacgagtt
     1921 cacccacccg cacttggcca agtacgggat ggccctcggc atcatggcgc tcggcgacaa
     1981 caaggtcacg agcacgcgcg agctgggagt caacgtccgc gacgcggtca tcgagccgcg
     2041 ctggaattca gacgacaacc ccggtgagcg cgacggcgac aggctctacg tagcgggcga
     2101 caacgaggtg ggcgtcttcg agctgagcaa caaacagcag atcgccacca ttccgggcac
     2161 gtacgaggcg atcgccgtcg atgaggaag
//
LOCUS       DATLCO010000070         3888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3804880, whole genome shotgun sequence.
ACCESSION   DATLCO010000070 DATLCO010000000
VERSION     DATLCO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804880"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VMZ33_01770"
     CDS             <1..268
                     /locus_tag="VMZ33_01770"
                     /inference="COORDINATES: protein motif:HMM:NF023134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HUR15988.1"
                     /translation="RHEGDPIAGAMARLAEAVARELTQGDSKRMRICANDECRWVFRD
                     YSPAGRRKWCDMSTCGNRAKAARHRERQKSKAGSPESASAEPVN"
     gene            489..614
                     /locus_tag="VMZ33_01775"
     CDS             489..614
                     /locus_tag="VMZ33_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15989.1"
                     /translation="MAAGAALIAPPIETPWRSLNSRLETPAGLQITLFQELDSTD"
     gene            complement(611..3124)
                     /gene="leuS"
                     /locus_tag="VMZ33_01780"
     CDS             complement(611..3124)
                     /gene="leuS"
                     /locus_tag="VMZ33_01780"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HUR15990.1"
                     /translation="MTRDIERYDPIEIEPRWQQRWDELGLHKTDLDDDSRPRYYVLTM
                     YDYPSGDLHIGHWFVKTPTDAISRYKRMQGFNVFFPVGFDSFGLPAENAAIRSGVHPR
                     AWTLANIDKMRRQLKSMGATWDWSAEVVTSDADYYRWNQWFFLKFLENDLAYREMAPV
                     DWCPKDQVVLAREQVIGTDRVCWRCGTQVIKRDLEQWFFRTTKYADEYLSYDGIDFPD
                     PIRVMQTNWVGRSEGAEVVFTTDGGDPLPVFTTRPDTLFGATFMVLAPEHPLVEKLTA
                     PDRKADVEAYLAATRRKSEIDRLSTDREKTGVPVGAFATNPINGEQIPIWIADYVLAG
                     YGTGAIMAVPAHDERDFSFAQKFGLSIRQVVAPPSVAESGAEADLSEAYSAHTADEVL
                     VNSGDFTGMLAVEGGRRITRALEKDGRGKSAVTYRLRDWLISRQRAWGTPIPVVYCKT
                     DQSCGIVPVPFEQLPVLLPEDFEFKPGGGNALETDERFTETTCPQCGGPGRRETDTMD
                     TFMDSSWYWWRYLSAGKTDGPIDTGRAAQWTPVAQYTGGAEHAVMHLLYARFFTKALN
                     DLGLVHEREPWLRLFNQGQILGADGERMSKSRGNVQDPDELVSRYGADTVRLFLMFMG
                     PWDQGGPWSPTGIEGVHRFLRRVWTVVLDPRGREAVEPSARITAEELQREAHRAVAKV
                     TEDHEGFRYNTMISVLMELTNAMFRGRGTDVVDSNEWDETCRLLLLMLAPIAPHISED
                     LWSRRLAGRGEEWRSVHQERWPEFDASLVAETEIELPIQINGKLRDVVNVPAGLSEIE
                     IEQIVMSRDKVQAQLARMEVVRVIHVRDRMVNVVVKPAR"
     gene            complement(3307..>3888)
                     /locus_tag="VMZ33_01785"
     CDS             complement(3307..>3888)
                     /locus_tag="VMZ33_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15991.1"
                     /translation="LAGQTHMSPDGDLVKLARDKCGWSYSFNRTLEMAQGQMSVRYEW
                     TGIVRVTDEGVISGTGTGFLRGSQGTCTYGDPPTEVPIDVTSSATFTFEFQGARAGDT
                     LTIEHPATTADVVVEGSFAPCMEAGPLAEFVIETALRDPALLPPAVVADRVGRTTERR
                     NGATSVTTLSPPPTPRPTPSPSSQPTIAGPSTP"
BASE COUNT          650 a         1316 c         1250 g          672 t
ORIGIN      
        1 tcgccacgag ggtgacccga tcgccggcgc catggcgcga ctcgccgagg cggtcgcgcg
       61 ggagctcacc cagggcgact caaagcgcat gcgaatctgc gccaatgacg agtgccgctg
      121 ggtgttccgc gactactcgc cggccgggcg tcgcaagtgg tgcgacatga gcacgtgcgg
      181 caaccgcgcg aaggccgcgc gccaccgcga acggcagaag tcaaaggccg gttcgcccga
      241 gtcggcatcg gccgagccag tcaactagtg gccgacgtgc ggccaaggcg acgcgtcgcc
      301 cagatgcgcc tcgtggtcga ggcggaggac tacgacgcgg ccgttgcctt ctaccgcgac
      361 gttctgggcc tcacgtaaat tcagcccaga agcgctacat cgacgagctg gaggtcggcc
      421 gtcaggtcgc acccaagtac cgcgtcgcgt tcgaggtcgc cgatgcaccc gcgacgaccg
      481 acgcgctggt ggccgccggc gccgcgctga tcgcgccgcc gattgagacg ccgtggcggt
      541 cgctcaactc gcggctggag acgcccgccg gcctgcagat cacgctcttt caggaactcg
      601 actcaaccga ctaacgcgcg ggtttgacca ccacgttgac catccggtcg cgcacatgga
      661 tcacgcgcac gacctccatg cgggcgagct gggcctggac cttgtcgcgt gacatgacga
      721 tctgctcgat ctcgatctct gacagccccg ccgggacgtt gaccacgtcg cgcagcttgc
      781 cgttgatttg aatcggcagc tcgatctccg tctcagccac gagcgaagcg tcgaactctg
      841 gccaccgctc ctggtgaacg gagcgccact cttcgccgcg ccctgccagc cgacgtgacc
      901 acagatcctc gctgatgtgc ggtgcgatgg gcgcgagcat cagcagcagc aaccggcacg
      961 tctcgtccca ctcgttcgaa tcgacgacgt ccgtgccgcg gccgcggaac atcgcgttgg
     1021 tcagctccat gagcacggag atcatggtgt tgtagcggaa gccttcatga tcctcggtca
     1081 ccttcgcgac cgctcgatgc gcctctcgtt gcagctcctc ggccgtgatg cgcgcgctcg
     1141 gctcgacggc ctcgcgaccg cgcggatcca gcaccaccgt ccacacgcgg cgcaggaacc
     1201 gatgcacgcc ctcgatgcct gtcggactcc acgggccgcc ctgatcccag ggtcccatga
     1261 acatcaagaa gagccggact gtgtcggcgc cgtagcgcga caccagctcg tcgggatcct
     1321 gcacattgcc ccgcgacttg ctcatgcgct cgccgtcggc gcccaggatc tggccctggt
     1381 tgaacaggcg cagccacggc tcgcgttcgt ggaccagccc caggtcgttc agtgccttgg
     1441 tgaagaaccg cgcataaagc aggtgcatta cggcgtgctc ggcgcctccc gtgtactgag
     1501 ccaccggggt ccactgcgct gcccgtccgg tgtcgatcgg cccgtcggtc ttgccggcgg
     1561 acaggtagcg ccaccagtac caggacgaat ccatgaacgt gtccatcgtg tcggtctcgc
     1621 gtcgcccagg cccgccgcac tgcggacacg tcgtttccgt gaaccgctca tcggtttcca
     1681 gcgcgttgcc gccaccgggc ttgaactcga agtcctcggg tagcaggacc ggcagctgct
     1741 cgaacggcac gggcacgatc ccgcacgact gatctgtctt gcagtagacg accgggatgg
     1801 gggtccccca ggcgcgctgc cgactgatca gccagtcacg cagccgatag gtaacggccg
     1861 acttgccacg accatccttc tccagcgccc gagtgattcg gcggccgccc tcgaccgcca
     1921 gcatccccgt gaagtcgccc gagttcacca gaacctcgtc cgccgtgtgc gccgagtagg
     1981 cctccgacaa atcggcctca gccccacttt cggccacaga tggaggggcg acgacctgcc
     2041 ggatcgacaa cccaaacttc tgtgcgaagg aaaagtcgcg ctcgtcgtgc gcgggcactg
     2101 ccatgatcgc gccggtgccg tagccggcca gcacatagtc agcgatccag atggggatct
     2161 gctcaccgtt gatcgggttg gtcgcgaacg ccccgaccgg caccccggtc ttctcccggt
     2221 cggttgacag gcggtcgatc tccgacttgc gtcgcgtggc cgccaggtag gcctcgacat
     2281 cggccttgcg atcgggtgcc gtcagcttct cgaccagcgg atgctccggc gccaggacca
     2341 tgaaggtggc cccgaagagc gtgtcgggcc gggtcgtgaa caccggcagc ggatcgccac
     2401 cgtcggtcgt gaaaacgacc tccgcgccct cggatcggcc gacccagttc gtctgcatga
     2461 cgcggatcgg atcggggaag tcgatcccgt cataggagag atattcgtcg gcgtatttcg
     2521 tcgtgcgaaa gaaccactgc tccaggtcac gcttgatgac ctgcgtgccg caacgccagc
     2581 agacacgatc ggtgccgatg acctgttccc gcgccaacac cacctggtcc ttgggacacc
     2641 agtcgaccgg cgccatctcg cggtaggcga ggtcgttctc caggaacttc aggaaaaacc
     2701 actggttcca gcggtagtag tcggcatcag aggtgacgac ctccgcgctc cagtcccatg
     2761 tcgcgcccat ggacttcagc tggcggcgca tcttgtctat gttcgccagc gtccaggcac
     2821 gcgggtgcac gccactgcga atcgcggcgt tctcggcagg cagcccgaag gagtcgaagc
     2881 caacggggaa gaacacgttg aagccctgca tccgcttgta gcgactgatc gcgtcggtcg
     2941 gcgtcttcac aaaccagtga ccgatgtgca ggtcgcccga cgggtagtcg tacatcgtca
     3001 ggacgtagta gcgcggccgg ctgtcgtcat cgagatcggt tttatgcagg ccgagctcgt
     3061 cccagcgctg ctgccagcgc ggttctatct cgatggggtc gtagcgctcg atgtcgcggg
     3121 tcatggcatg gccctcgtta ggggtggtga caggatggga cggatctgtt ttcgggtcgc
     3181 gcagcgcgtc gttaagcgcc ggcgaccccg agaagtacga ggtcacgacg cgtggggcag
     3241 acccagactc gcctgtgtgc catgaggccg tgatcttaac cgcagatcag gtttgtgggc
     3301 agcccctcac ggcgtcgatg ggccggcaat agtcggctgg ctggaagggc tcggcgtcgg
     3361 ccgcggcgtg ggtggggggc tgagggttgt gaccgacgta gctccatttc tccgctccgt
     3421 cgtccggccc acgcggtcgg ccacgacggc cggcgggagt agagcgggat ctcgcagtgc
     3481 cgtctcgatg acgaactccg cgagcggccc cgcctccatg cacggcgcaa agctgccttc
     3541 gacaactacg tctgccgtgg tcgcggggtg ctcgatcgtg agtgtgtccc cggcccgcgc
     3601 cccctggaac tcgaacgtaa acgtggcgct ggacgtgaca tcgattggaa cttccgtggg
     3661 cgggtcgccg tatgtgcacg tcccctggga accgcgtagg aatccggtcc ccgtgccgct
     3721 gataacgccc tcatctgtga cgcggacgat cccggtccac tcgtaccgca ccgacatttg
     3781 accctgcgcc atctcaagcg ttcggttgaa ggaatacgac cagccgcatt tgtctcgtgc
     3841 cagcttcacc agatcgccgt cggggctcat gtgggtctga ccggcgag
//
LOCUS       DATLCO010000071         5576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3020911, whole genome shotgun sequence.
ACCESSION   DATLCO010000071 DATLCO010000000
VERSION     DATLCO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020911"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VMZ33_01790"
     CDS             complement(<1..486)
                     /locus_tag="VMZ33_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR15992.1"
                     /translation="MPRTIAVVSTETSLSVLHAIVPVRGVTSAKTRLGSALDSEERQV
                     LALGLVSHTLATLEAWAACRKIHVVSDDAVTLRAIGRDRDKVDLLRQFSVGLNAAVTL
                     ARDRALAQGATAVLILPADLPLLSVEALVATLDGADAALAAGDGHPVVVIAPSDARGG
                     TN"
     gene            complement(526..1488)
                     /gene="cofD"
                     /locus_tag="VMZ33_01795"
     CDS             complement(526..1488)
                     /gene="cofD"
                     /locus_tag="VMZ33_01795"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HUR15993.1"
                     /translation="MTDRTRVTVIAGGFGGARMAHGFALLPDDVELTVVINTADDTEL
                     HGLRISPDLDTVMYTLAGVANNETGWGVANETWSAAEMLAGYGEPTWFRLGDRDLATH
                     VVRTARLHAGERLTDVTRDLAAALGVTATLLPMTDDEVRTKILTPDGWLDFQDYFVRR
                     HHSDPVEDVRFDGMETARATAEALEAITEADLIVIAPSNPFVSVGPVFALPGIRDAML
                     AADAPILAVSPIVGGAALRGPAANMMRTLGAGAAATAAGIARVYATRYPGVVDTLVID
                     DADAADAEAVAATGIAYAAAGIVIADEHSRRRLALNLLRLSLQP"
     gene            complement(1562..2566)
                     /locus_tag="VMZ33_01800"
     CDS             complement(1562..2566)
                     /locus_tag="VMZ33_01800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HUR15994.1"
                     /translation="MATEARSMKPQFGYAAMLEQFHPTELIDYCVMAEENGFSGVMAA
                     DHLQPWVPQQGQAAFVWAFMAAVAERTKGDVGPGVTCPTFRTHPVVIAQAAATLAAMY
                     PGRFWLGLGSGEALNEHVVASYWPEAPERINRMFEAIEMFNRLFTGKDIKHDGRFYKM
                     ETVRLWTLPETPPDIYVATAGPITAKRTGQAADGIITVGAPEGKLEMIRAKAREGREE
                     AGQDPATFKFILQLHMSWAPTDEEALNNAMTEWPNGGMKFPKQDIRSPLDFEQMAKLV
                     RPEDFEGRMLISSDLDTHRREIQKYADMGFDRIYLHNVGRNQKEWIEAFGREVLPNIT
                     "
     gene            complement(2566..2997)
                     /locus_tag="VMZ33_01805"
     CDS             complement(2566..2997)
                     /locus_tag="VMZ33_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUR15995.1"
                     /translation="MSVIATESLLSDDVRSFLDCSPPRYATIATVNPDGSPHQTVIWF
                     ELRDDLIIVNSRVGRRWPSNLLRNSRAAMCVYDRADGVAIDCKVVETIDGEEALAAIS
                     AMAFRYCDTEEAEELIVEWRRQRRLTFVLRPVRIHTAGDPS"
     gene            complement(3025..3693)
                     /locus_tag="VMZ33_01810"
     CDS             complement(3025..3693)
                     /locus_tag="VMZ33_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HUR15996.1"
                     /translation="MSSYLELADWRRRVAEIYADWRRDSVTDAAAATLQLRARRTELF
                     RSHSQSPIPIDERARFGGLSHWPYDPALRMTAAFESDDAALADPHAEEPAAVGGLGQI
                     QLPTSGASPFKFRRVGSVRLSGPLAGTTLPVYWMLGYAGGVFVPFRDATSGTETYGAG
                     RYLLDTVKGADQGVDPASGELVLDFNLAFHPSCTYDPKWNCPLAPPESHLSVPVSAGE
                     RLPG"
     gene            complement(3710..4624)
                     /locus_tag="VMZ33_01815"
     CDS             complement(3710..4624)
                     /locus_tag="VMZ33_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR15997.1"
                     /translation="MRMFRTLALGAALLVLAGACSLGGGSKPSVRIGSVGFDEAKVIA
                     EIYAQVLEKNGYTVERKFGLGERPVVAPALESGQFDLQPEYIGSRLVYEGGSPSGDSA
                     ATFADLQERLLAKNLTALNYTPGVDTNAFVVRAETAQELGLATLSDAAAVQDQLEWGL
                     AADCPTNALCGAPGGALAQYGITAETIAAGTLLASCSTPMADALKAGTIDVAELCSTQ
                     PDILVNGWVLLEDDLHTQPAENVAPIVRNDFLAKVGDRAAFDKIFNDVSAQIDTATMT
                     DLYKQVAVDLKDVGEVAETWLRAEGFVE"
     gene            complement(4711..5430)
                     /locus_tag="VMZ33_01820"
     CDS             complement(4711..5430)
                     /locus_tag="VMZ33_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR15998.1"
                     /translation="MNIVDQTIAWLADPAHWQGPTGVPARMIEHAAISGVSLLIAIAI
                     ALPVGLYIGHTGRGVGIAVNSANLWRALPSLAVIGIVLPITAALDPRAGFLVYPTVVA
                     MVVLGVPPILVNTYAGISGVDHDVIEAARGQGLSNRQILRRVEIPLAVPVVLAGIRSS
                     AVQIIATATLGAVFGFGGLGRYLVDGFAQFSLGGAAQMMAGVVLVATLVMATDVAFRV
                     TQRALTPKGSKQKRSTVASAT"
BASE COUNT          954 a         1805 c         1871 g          946 t
ORIGIN      
        1 gttcgtgcca ccgcgcgcgt cggagggggc gatgaccacc accgggtggc cgtcacccgc
       61 cgcgagggcg gcatcggcgc cgtcgagcgt ggcgaccagc gcctcgacgc tgagtagcgg
      121 caggtcggcg gggagaatca ggacagctgt tgccccttgg gcgagcgcgc ggtcacgcgc
      181 cagggtcact gcggcgttca agcccacgct gaattgccgg agcaggtcaa ccttgtctcg
      241 gtcgcggcca atggcgcgca gggtcacggc gtcgtcgctg acgacgtgga tcttgcggca
      301 ggcagcccac gcctcgagcg tggccagtgt gtggctcacg agccccaggg cgagcacctg
      361 acgctcctcg gaatcgagcg ccgaaccgag gcgcgtcttc gctgatgtga ctccgcgcac
      421 cggcacgatc gcgtgcagaa ccgacaggct ggtctccgtg ctcaccacgg cgatggtacg
      481 cggcaatccg tgtgccataa ctcggacgac cctgagtgtg gggcgctagg gctgtagcga
      541 caacctcagc aggttcaacg ccaggcggcg tcggctgtgt tcatcggcga tgacgatgcc
      601 ggctgctgcg tatgcaatcc ctgtcgcggc caccgcttca gcatccgcgg cgtcggcatc
      661 gtcgatgacc agcgtgtcga cgacacctgg gtagcgcgtc gcatacaccc gggcgatgcc
      721 ggcggcggtc gcggcggcgc cggcgcccag agtccgcatc atgttcgcgg ccgggccgcg
      781 gagcgcggcg ccgccgacga tcgggctgac ggccaggatt ggggcgtcag ccgccagcat
      841 cgcatcccgg atccccggca gcgcgaagac cgggccgacg ctcacgaacg ggttggaagg
      901 agcgatcacg atcaggtcag cttcggtgat cgcttcgagt gcttcggcgg tggctcgggc
      961 tgtctccatg ccgtcgaatc gaacgtcctc aactgggtcc gaatgatggc ggcgcacgaa
     1021 gtaatcctgg aagtcgagcc atccgtcagg ggtgagtatc ttggtgcgca cctcgtcgtc
     1081 ggtcatcggc agaagagtgg ccgtcacgcc gagcgccgcg gcgagatcgc gcgtgacgtc
     1141 ggtgagacgc tcgccggcgt gcagccgggc ggtgcgcacg acatgagtgg cgaggtcgcg
     1201 atcgccgaga cggaaccacg tcggctcgcc atagccggcg agcatctccg ccgcggacca
     1261 ggtctcgtta gccacgcccc agcccgtctc gttgttcgca accccggcga gcgtgtacat
     1321 gaccgtgtcc aggtccggcg agatacgcag gccgtggagc tccgtgtcgt cggccgtatt
     1381 gatgaccacc gtcagctcga cgtcgtcggg aagaagcgcg aagccgtggg ccatgcgcgc
     1441 ccccccgaag ccgccggcga tcacggtgac tcgggttcga tcagtcactg tgcaagtgtg
     1501 cattgcgggt ccgtcggccg acggtattca ttggggcggg cgcgccacgc aggctcacgc
     1561 cctaggtgat gttcgggagc acctcgcgcc cgaacgcctc aatccactcc ttctggttcc
     1621 gccccacgtt gtgcaggtag atgcggtcaa agcccatgtc ggcgtacttc tgtatctcgc
     1681 gccgatgggt atcgagatcg gacgagatga gcatccggcc ctcgaagtcc tccggtcgga
     1741 cgagcttggc catctgctcg aaatcgagtg gcgagcgaat gtcctgcttg ggaaatttca
     1801 ttccgccgtt gggccattcg gtcatggcgt tgttgagtgc ctcttcatcg gtcggcgccc
     1861 atgacatatg gagctgcagg atgaacttga aggtggctgg atcctgaccc gcctcctcgc
     1921 gcccctcgcg cgccttggcg cggatcatct cgagcttgcc ctcgggcgcg ccgacggtga
     1981 ttatcccgtc cgctgcctgg ccggtgcgct tggcggtaat cggccccgcc gtggcaacgt
     2041 agatgtcggg cggcgtctca ggtagcgtcc agagtcggac ggtctccatt ttgtagaagc
     2101 ggccgtcgtg cttgatgtcc ttgccggtga agaggcggtt gaacatctcg atcgcctcga
     2161 acatccggtt gatgcgctcc ggcgcctcgg gccaatagga cgccacgacg tgctcgttca
     2221 acgcctcacc agagccgagg ccgagccaga agcggccggg atacatcgcc gcgagagtgg
     2281 ccgcggcctg ggcgatcacc accggatggg tgcggaacgt cgggcaggtc acgccggggc
     2341 cgacatcacc cttggtgcgt tcagcgaccg cggccatgaa cgcccacacg aacgcggcct
     2401 ggccctgttg cgggacccac ggctggaggt ggtcggcggc catcaccccg gaaaaaccgt
     2461 tctcctcggc catgacgcag tagtcgatga gctcggtcgg gtggaactgc tcgagcatgg
     2521 ccgcgtagcc gaattgaggt ttcatagagc gcgcctcggt tgccatcagg aagggtctcc
     2581 ggctgtgtgg attcgaactg gtcggaggac gaatgtcagc cgccgctgtc ggcgccactc
     2641 gacgatgagc tcctcggcct cctccgtgtc gcagtagcga aaggccatcg cggagatggc
     2701 ggccagggcc tcttccccgt cgatcgtctc gacgactttg cagtcaatgg cgacgccgtc
     2761 ggcgcggtcg tagacgcaca tcgcggctcg cgaatttcgc aggagattcg acggccaacg
     2821 acgaccgacc cgcgagttga cgattatcag gtcgtcgcgc agctcgaacc agatgacggt
     2881 ctgatgggga ctgccgtcgg gattgaccgt cgcgatcgtc gcgtagcgcg gcggcgagca
     2941 gtcgaggaat gagcggacat cgtcgctcag cagcgactcg gtagcgatca cggacaccta
     3001 ccgatgttag cgatcggctc ctggttaccc cggaagtcgt tccccggcgg acacgggcac
     3061 cgatagatgc gattccggtg gggcgagggg gcagttccac ttcgggtcgt aggtgcatga
     3121 ggggtggaac gcgaggttga agtcgagcac aagctccccg ctggccgggt ccacgccctg
     3181 gtctgcgccc ttgactgtgt cgagcaaata acgtccggca ccgtaagttt cggtcccgga
     3241 cgtagcgtcc cggaacggaa cgaagacgcc tccggcgtac cccagcatcc aatagacagg
     3301 caacgttgtc ccggcgagcg gccccgagag ccgcacgctg ccgacgcgcc ggaatttgaa
     3361 cggtgacgcc ccactggtcg gcagctgaat ctggcccagc ccaccgacgg ccgcgggttc
     3421 ttcggcatgt ggatccgcga gggccgcgtc gtcggactcg aaggccgccg tcatgcgcaa
     3481 ggcggggtcg tacggccaat gggagagccc gccgaagcgg gccctctcat cgatcggtat
     3541 gggtgactgc gaatggctac gaaacagctc cgtgcgtctc gcgcgcagtt gcagtgttgc
     3601 ggcggccgcg tcggtcaccg agtcacgccg ccaatcggcg tagatctcgg cgacgcgccg
     3661 tcgccagtcg gcgagctcga ggtacgaact catcgtgggg agagctcggc tactcgacga
     3721 aaccttctgc cctaagccat gtctcggcaa cctcgccaac atccttgagg tcgacggcga
     3781 cctgcttgta gaggtcggtc atcgtcgccg tgtcaatctg cgccgacacg tcgttgaaaa
     3841 tcttgtcgaa cgcagcacga tcaccgacct tcgcgaggaa atcgttgcgc acgattggcg
     3901 cgacgttctc cgccggttgc gtgtgcaggt cgtcttcgag cagcacccag ccgttgacca
     3961 ggatgtcggg ctgagtcgag caaagctcgg cgacatcgat ggtgcccgct ttgagcgcgt
     4021 cagccatcgg cgtactgcac gacgctagaa gagttccggc tgcaatcgtt tcggcggtga
     4081 tgccgtattg cgcgagagcg ccacccggtg cgccgcaaag ggcgttggtc gggcagtctg
     4141 cagcaaggcc ccactcgagc tggtcctgga ccgccgctgc gtcgctcaac gtcgcgaggc
     4201 cgagctcctg cgctgtttcg gcgcgaacga caaatgcatt tgtgtcgaca ccgggcgtgt
     4261 agttcagcgc cgtgagattc ttggcgagga gcctctcttg cagatcggcg aacgtcgcgg
     4321 ccgagtcgcc gctgggcgaa ccgccctcgt ataccagtcg gctgccgatg tactccggct
     4381 gaaggtcgaa ttgacccgac tccagtgcgg gggcaaccac cgggcgctcc cctaagccga
     4441 acttccgctc aactgtgtag ccattcttct cgagcacctg cgcgtagatc tcggcgatga
     4501 ctttggcctc gtcgaatccg acagagccga tcctgacgct tggcttgctg cccccaccca
     4561 ggctgcacgc gcctgccagc acgagcaacg ctgcaccaag ggcaagcgtg cggaacatcc
     4621 gcatgggtaa cctactcctc tcattgttcc ggttagcaca cgaaccggcc gggcgactat
     4681 acaggcaaca tctcggatgc cactacgtca ttacgtggct gacgccactg tggatctctt
     4741 ctgctttgag cctttcggcg tgagcgcgcg ctgcgtgacg cggaacgcga cgtctgtggc
     4801 catgacgagc gttgccacga ggacgactcc agccatcatc tgcgccgcgc caccgaggct
     4861 gaactgggcg aacccgtcta cgagatagcg gcccagcccg ccgaagccga acacggcgcc
     4921 gagggtcgca gtggcaatga tctgcaccgc gctggagcgg atcccggcca agacgacggg
     4981 cacggcgagc gggatttcga cccgcctcaa gatttgacgg ttcgacagac cctggccgcg
     5041 tgccgcttct atgacatcgt ggtccacgcc gctgatgccg gcgtacgtgt tgacgaggat
     5101 cggcggaacg ccgagtacga ccatcgccac aacagttgga tatacgagga agccggcacg
     5161 cgggtcgagg gccgcggtga tcggcagcac gatcccgatg acggcgagcg atggcaatgc
     5221 gcgccacagg ttcgcggaat tgacggcgat gcccacgcca cgaccggtat ggccgatgta
     5281 caggccgacg ggaagcgcga tggcgatcgc gatcagcagc gacacccccg agatcgcggc
     5341 gtgctcgatc atccgcgccg gcaccccggt cggcccctgc cagtgcgcgg ggtcggccag
     5401 ccacgcgatc gtctgatcga cgatgttcac gcggccgcgt ccccgtcggc ccgcgccgca
     5461 gcccgtgtcc acggcgtgat cgaacgttgc acacgaagga gcagccagtc gacgatgaag
     5521 gcaagcacaa tcgatggcac cgcaccgaac cacagctcgg tggggaaacc gcgccg
//
LOCUS       DATLCO010000072         2607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6747225, whole genome shotgun sequence.
ACCESSION   DATLCO010000072 DATLCO010000000
VERSION     DATLCO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6747225"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2344)
                     /locus_tag="VMZ33_01825"
     CDS             complement(<1..2344)
                     /locus_tag="VMZ33_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HUR15999.1"
                     /translation="MPSGIALGLALSLAIGASTVVARLPAAGPDGFRTLTGAAAREFT
                     IPGDVRLVSRWSDSRANLTFERYQQYAPVSGAHVDGGQLTVGRRGGRIELVVGSHYNL
                     FAAVNVVRLSAATAAATAAASSAVASLLDTAAADAVATGAELRLNPRTGRLFYRVAND
                     AISALVYADIDATTGEVINAWSGLDALDPGQGTGVKGDTKNLTGLTSQTGSDPWKLQS
                     ANGRLVTRDARGNSRLWDLVDFSDSDNAWAAATQRAAVDAQYYASKTDGWYLAEMGFD
                     LLAEHYLPSPVEGQPDVLQCDLPAITSVVHYDDTPGDGFGYDNAFWHPEREMMVYGDG
                     DGVETGPFSGAQDVVSHELSHAVTTCRAPLTYYSSSGALNEGFSDIMAAAMEWEFAEP
                     NSSGCRRLTSQTACPDWWIGEDSIISGTNFGFRNLARPQSTYNNPSHWADRYRGSFDN
                     RGVHYNSLLPGHAFYLMSMGGRNARCVGATDTKADCDVVVPSIGIKDAAHIFFNAWGT
                     LPEDADYCDAQAATIVAAQTVFPGSDLHLASAVLGWAAVGPRTDYPNAIGNACAGNLP
                     KLTLSTRSVALAGGQSAEIDVTNLGQFEALSVSAQGPVAAELVASDPDTVLVSVPEGT
                     APGVYYVMFTGTTASVTTTRYAVATVVVDATPPTASVTQVALIGSGQIAQSGTIQLQV
                     SWSAADDLSGVADADLEWTSTGSEPWPVLVDTPPANGQSAVIVGGNEYSFRVRALAGV
                     GHETLSQESGPWQIARSQENAATYTKTWIDRVVADYWGGGS"
BASE COUNT          456 a          843 c          860 g          448 t
ORIGIN      
        1 tggagccgcc gccccaataa tccgccacga cgcggtcgat ccaggtcttc gtgtaagtcg
       61 cggcgttctc ctgcgagcgc gcgatctgcc acggaccaga ctcttgcgac agtgtttcgt
      121 ggccgacgcc agcgagcgcc cgaacgcgga agctgtactc gttcccgcca acgatgacgg
      181 cgctctgtcc gttggcgggt ggcgtgtcga ccaaaaccgg ccacggctcg ctgccggtgg
      241 acgtccattc cagatccgcg tcagcgacgc cgctcaggtc atccgctgcc gaccaggaca
      301 cctgcaattg gatcgtgccc gattgggcaa tctgacccga accgatcagg gcgacctgcg
      361 tcacgctggc tgtcggcggg gtggcgtcga cgacgacagt cgcgacggca tagcgagtcg
      421 tcgtgaccga cgcggtcgtg cccgtgaaca tgacgtagta gacgccgggc gccgtaccct
      481 cgggcacgga tacgaggacg gtgtcagggt cagatgccac gagctccgca gctaccggcc
      541 cttgggcaga caccgaaagt gcctcgaact ggccgagatt ggtgacgtcg atctctgcgg
      601 actggccgcc agctagcgcc acggaccttg tgctgagggt cagcttgggc aggttgcccg
      661 cgcaagcgtt gccgatggcg ttcggatagt cggtacgcgg cccaaccgcc gcccaaccga
      721 ggactgctga cgcgaggtgg agatccgagc caggaaagac ggtttgggcc gccacgatgg
      781 tcgcagcctg ggcatcgcag tagtccgcgt cctcaggaag cgtgccccac gcgttgaaga
      841 agatgtgggc agcatccttg atcccgattg acggcaccac gacgtcgcag tcggccttcg
      901 tgtctgtcgc gccgacgcaa cgcgcgtttc ggccgcccat cgacatcagg tagaaggcgt
      961 gacccggcag caggctgttg tagtggacgc cgcgattgtc gaatgagccg cggtagcggt
     1021 cggcccagtg cgacggattg ttgtaggtgc tttgcggccg cgcgaggtta cggaagccaa
     1081 agttcgtgcc cgagatgatc gagtcctcgc cgatccacca atcagggcat gcggtctggc
     1141 tggtcagccg tcggcagccc gaactgttcg gctcggcgaa ctcccactcc atggcggcgg
     1201 ccatgatgtc ggagaagcct tcgttcagcg cacctgatga cgagtagtaa gtcaacggcg
     1261 cgcggcaggt cgtgactgcg tggctcagct catgagacac aacatcctgg gcgcccgaga
     1321 agggcccggt ctcgacgccg tcaccgtcgc cgtagaccat catctcgcgc tccggatgcc
     1381 aaaaggcgtt gtcgtagccg aagccgtcgc cgggtgtgtc gtcgtagtgc acgacgctgg
     1441 tgatcgccgg caggtcacat tgcagcacgt ccggctggcc ttcaaccgga ctcggcaagt
     1501 agtgctcagc cagaaggtca aagcccatct cagccaggta ccaaccgtcg gtcttcgacg
     1561 cgtagtactg ggcatccaca gccgcgcgct gggtggccgc ggcccatgcg ttgtctgagt
     1621 cactgaagtc gaccaaatcc cacaacctgg agttgcctct tgcgtcgcgt gtgacgagtc
     1681 ggccgttggc agactgaagc ttccagggat ccgaccccgt ctgactcgtg aggccggtga
     1741 ggttcttggt gtcacccttg accccggttc cctgccccgg gtcgagcgcg tcgagacccg
     1801 accaggcgtt gattacctcg cccgtcgtgg catcaatatc ggcgtagacg agggccgaaa
     1861 tggcgtcatt cgcgacgcgg tagaagagcc ggcccgtgcg cgggttgagt cgtagctccg
     1921 cgccggtggc gaccgcgtcc gcggcggcgg tgtcgagcaa cgaggccacg gccgacgaag
     1981 cggcagctgt cgctgccgcc gtggcggcac tcagccgcac cacgttgacc gccgcgaaga
     2041 ggttgtagtg cgagccaacg acgagctcga tgcggccgcc gcgccgtcca acggtgagct
     2101 ggccaccatc gacatgcgcg cctgataccg gcgcgtactg ctggtagcgc tcgaaggtca
     2161 ggttggcgcg cgaatcactc cacctcgaca cgaggcgaac gtcgccggga atcgtgaatt
     2221 cgcgcgcggc ggcaccggta agggtccgga agccgtcggg acccgcggcg ggcagccggg
     2281 ccacgaccgt gctcgcgccg atcgcgaggg acagcgccaa tccgagcgcg attccggatg
     2341 gcacgaggcg acggctatgc ctcaagtgtt gactccctgt gtctcgaccg actggcggtc
     2401 gaacccccgc ctgaccctaa cggccagccc ctccactaac gcatcaccca attgccgggg
     2461 tgcatcagca gattgtcacg ccccggctga atgttatttg ggctacttgt caccttcgac
     2521 ggcgatcttg tccaggtact gggcgatctc cggaacagcg acaagacgct tgtggaaata
     2581 ctcgttgacc gcgttcatcg tctcgac
//
LOCUS       DATLCO010000073         4036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6002153, whole genome shotgun sequence.
ACCESSION   DATLCO010000073 DATLCO010000000
VERSION     DATLCO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6002153"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="uppS"
                     /locus_tag="VMZ33_01830"
     CDS             <1..679
                     /gene="uppS"
                     /locus_tag="VMZ33_01830"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HUR16000.1"
                     /translation="QPRHVAIVMDGNRRWARMHGVSEGEGHAAGVDAIRPIVEHAGDL
                     GIEALSIFAFSRENWNRASDEVQTLFALMEAAIRRETPDLIRQGVRIRVLGRLQELPE
                     TTRASIQESVDATAEGARLTLNIAFNYSGRSEIVDALQRSVADGVPADKITEDDIESR
                     LYTANLPELDLLIRTGGEQRISNFMIWQAAYAELYFSDRLWPDFDPAEFDTALAEFAR
                     RSRRFGR"
     gene            768..1652
                     /locus_tag="VMZ33_01835"
     CDS             768..1652
                     /locus_tag="VMZ33_01835"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HUR16001.1"
                     /translation="MRQRLISAAVLVPVVAFVFLVGQPWLSVGIALLGVMAAWETARL
                     VHLAGLPAFRWLAISAPVLGVLAFETILRPGGIELGWLLIPPALAIWSIAAAAPALAN
                     PDPKNGFLVWVGTLFAGLFPALIAFLAGIAVWGYGSVLPGESEHQTLFNLDLGRHWLL
                     VLIATVWTFDSFAYLSGKFFGRGRFMYHISPAKTWSGVIGGTIAAMVVCGALFLVTGL
                     SPLWGGVLGIALALSGQAGDLAESMLKRAAAVKDSGRILPGHGGFLDRVDSFLFAAPT
                     LYAAVTIAALLQASGGTP"
     gene            1649..2812
                     /gene="dxr"
                     /locus_tag="VMZ33_01840"
     CDS             1649..2812
                     /gene="dxr"
                     /locus_tag="VMZ33_01840"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HUR16002.1"
                     /translation="MTDARERTRVAILGSTGSIGRQALDVLAAHGDRFEVVGLAAGRG
                     GEAFEAQVAQFRPAHVATAADGTQALADMAAAPEVDMLIVATTGIVSLVPTLAALQGG
                     KIVATANKETLVAGGHLVMPLARKLAERADVRVTNALAYLRPIDSEHCAIWQCLNGEM
                     RSQVTRLILTGSGGPFREWQPERRVTVTPEDALKHPTWRMGPKITIDSATLMNKALEV
                     IEARWLYDVEDDQVDVVIHPQSVIHSMVEFADGSIKAQLGLPDMRIPIQYAVTFPDHL
                     PSPAPRITGVDFPTLTFEPVDARLFPALAIGREAGRRGPAASAALIAADDVAVARFLN
                     GTLTFTAVSDLCAAAVDQFGASADPNLEQLLALDAEVRLWAATWGVPATSKAS"
     gene            2813..3967
                     /locus_tag="VMZ33_01845"
     CDS             2813..3967
                     /locus_tag="VMZ33_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HUR16003.1"
                     /translation="MPDLFVGLGNGLIDVVLLLVILVGLVVIHELGHFVAARRAGVTV
                     HEFGIGFPPRAKVMGKDKHGTLYTLNWLPIGGFVRLEGEEGESDDPHAFIRQRLPTRL
                     VILLAGVAMNAVLAFVLLTLITALADPSSAVRVLSVQDGSPAQTMGLRGGVQTGTTSD
                     SQGNAIPIYDNSGDLILAVDGVKSAWFDLPNGPQGLTLYVRSRPATPVTLTVERGGQI
                     VELTGTTRTAAEIAENKGPLGFTPQYEPGPTISRDPVQAVQIGAQRTLDAATLILRAL
                     GDFINNLASPPVSGPVGIVQAIGTVRTEAPPVFLVYFIALLSANLAVVNVLPLPPLDG
                     GRVAVSLVRALVGERISLAAERFVYFAGFVFLMALLAWVTLFDTGILQRT"
BASE COUNT          595 a         1379 c         1305 g          757 t
ORIGIN      
        1 ccagccacgc cacgtggcga tcgtcatgga cggcaaccgg cgttgggcgc ggatgcacgg
       61 cgtcagcgag ggcgagggtc acgccgccgg agtcgacgcc atccggccca tcgttgagca
      121 cgccggcgac cttggcatcg aagccctctc gatcttcgct ttttcgcgcg agaactggaa
      181 ccgcgccagc gatgaggtcc aaacgctatt cgccctgatg gaggcggcca tacgccgcga
      241 gacgcctgac ctgatccgcc aaggggttcg gatccgtgtt ctcggcaggc tccaggagct
      301 gccggagacc actcgagcgt cgatccagga gtcagtcgac gccaccgctg agggcgctcg
      361 gctgaccttg aacatcgctt tcaactactc cggccgatcc gagatcgtgg acgcgctcca
      421 gcgctcagtc gcggacgggg tgccggccga caagatcacc gaagacgaca tcgaatcgcg
      481 cctctacacg gccaacctgc ccgagctgga cctcctcatc cgcaccggcg gtgagcagcg
      541 catctccaac ttcatgatct ggcaagccgc ctacgccgag ctctacttct ccgaccggct
      601 ctggcccgac ttcgaccctg ccgagttcga taccgcgttg gccgaattcg cccggcgctc
      661 gcgtcgcttc ggccgctgaa catgttcatc tccgcagtcg gccttgggcc gctgccgcgc
      721 gcctccggcg aggctccgtc gccgcggctc ctcgccaagg cttgcgtgtg cgccagcggc
      781 tgatcagcgc ggccgtgctc gtgccggtcg tggccttcgt cttcctggtc ggccagccgt
      841 ggctatccgt ggggatcgcc ctgttgggcg tgatggcggc gtgggagacg gcacgactcg
      901 ttcaccttgc cggtttgccg gccttccgtt ggctggccat cagcgcgccc gtgcttggag
      961 tgctggcgtt cgaaacgatc cttcgacccg gcggtatcga gctcggctgg ctgctcatac
     1021 cgccagcgct ggccatctgg tcgatcgctg ccgcggcgcc ggccctcgcc aacccagatc
     1081 ccaagaacgg cttcctcgtg tgggtcggca cgctcttcgc cggattgttt ccggcgctga
     1141 tcgctttcct tgccggcatc gccgtatggg gctacggcag tgtgttgccc ggcgagtccg
     1201 agcaccagac gttgttcaac ctcgatctcg gtcgacactg gctattggtg ctcatcgcca
     1261 cggtctggac gttcgacagc ttcgcctacc tctccgggaa gtttttcgga aggggtcggt
     1321 tcatgtacca catctccccg gccaaaacgt ggtcgggcgt gatcggcggc acgatcgcgg
     1381 ccatggtcgt ctgcggcgct ctcttccttg tcacaggcct cagtcccctc tggggcgggg
     1441 tcctcggcat cgctctggct ttgagcggcc aggcagggga tcttgcggag tcgatgctca
     1501 agcgcgcggc agccgtcaag gattccggtc ggatcctgcc tggccatggc gggtttcttg
     1561 atcgagtgga ttcgttcctc ttcgcagcac ccacccttta tgcggcagtg acgattgcgg
     1621 cgctgctaca ggccagcggt ggcactccgt gactgacgcg cgggaacgca cccgcgtggc
     1681 catcctgggc agcacgggct cgatcggccg acaggcgctc gatgtcctcg ccgcgcacgg
     1741 cgacaggttc gaggtcgtgg gcctcgccgc aggtcggggc ggggaggctt tcgaggcgca
     1801 ggtcgcgcag tttcgacccg cgcacgtcgc cacggcggca gacggcactc aggctttggc
     1861 ggacatggcc gcggcaccag aggtcgatat gctgatcgtc gccacgacgg ggattgtcag
     1921 cctcgtgcca acgctcgcgg ccctgcaagg gggcaagatc gtggccaccg ccaacaaaga
     1981 gaccctcgtc gccggcggcc acctggtgat gccactcgcg cggaagctgg ctgagcgtgc
     2041 ggatgtccgg gtgacgaacg cgctcgccta tcttcgcccg atcgactcag agcactgcgc
     2101 gatctggcag tgcctcaacg gcgagatgcg gtcccaggtg acgcgcctga tcctgactgg
     2161 ctcgggcggg ccctttcgcg agtggcagcc ggagcgccgc gtgacggtca ctcctgaaga
     2221 cgcgctcaag catccgacct ggcgaatggg tcccaagatc acgattgatt cggcgacgct
     2281 catgaacaag gcgctcgagg tcattgaggc acgctggctg tacgatgtcg aggacgatca
     2341 ggtcgacgtc gtcatccacc cgcagagcgt catccactcc atggttgagt tcgcggacgg
     2401 ttcgatcaag gctcagctgg gcctccccga catgcggatc ccgatccagt acgccgtgac
     2461 atttcccgat catctgccgt cgcctgcgcc gcgaattaca ggtgtggact ttccgacgct
     2521 gacgtttgag cccgtcgacg cacggctctt ccccgcgctg gccataggcc gcgaggcggg
     2581 ccgacgtggc ccggccgcga gcgcggccct tattgccgct gacgacgtcg ctgtcgcccg
     2641 cttcctcaat ggcaccctta ccttcacggc cgtgtccgac ctatgcgccg ccgcagttga
     2701 ccaattcggc gcttccgccg atcccaatct cgagcagctg ctcgccctgg atgcggaggt
     2761 ccgtttgtgg gccgcaacct ggggcgtgcc tgcaacgtca aaggcttcgt aaatgccaga
     2821 tcttttcgtc ggcctcggca acggcctcat cgatgtcgtc ttgcttctcg tcatcctcgt
     2881 cgggctcgtg gtcatccacg aactcggcca cttcgtcgcg gcgcggcgcg ccggagtcac
     2941 cgtccatgag ttcggcatcg gcttcccgcc acgtgccaag gtcatgggca aggacaagca
     3001 cggcacgctc tacacgctga attggctgcc gatcggcggt ttcgttcggc tggagggtga
     3061 agaaggcgag tcggacgacc cgcacgcgtt cattcgccag cgcctgccaa ctcgcctcgt
     3121 gatcctgctc gcgggcgtgg ccatgaatgc cgtcctggcc ttcgtcctgc tgaccctgat
     3181 taccgcgctc gccgacccga gctcggccgt gcgcgtgctg agcgtccagg acggctcgcc
     3241 cgcgcagacg atgggcctcc ggggtggagt tcagacgggc acgacctccg actcccaggg
     3301 caacgcgatt ccgatctatg acaactcggg tgacctgatc ctggccgtcg acggcgtgaa
     3361 gtccgcgtgg ttcgacctgc cgaatggacc gcagggcctc acgctgtacg tccgttcgcg
     3421 gccagcgacg ccggtcactc tgacggtcga acgcggcggg cagatcgttg agctcaccgg
     3481 taccacgcgg accgccgcgg agatcgccga gaacaagggt ccgctggggt tcaccccgca
     3541 gtacgagccc gggccgacga tctcacgcga cccggtgcag gcggtccaaa tcggcgcgca
     3601 gcgaaccctg gatgcggcga cgttgatcct gcgcgccctc ggcgacttca tcaacaacct
     3661 tgccagtccg cccgtttcgg gcccggtggg gatcgttcag gcaatcggca ccgtccggac
     3721 cgaggcgccg cccgtgtttc tcgtgtactt catcgcgttg ctgtcggcca atctggccgt
     3781 ggtcaacgtg ctgccgttgc cgccgctcga tggtggccgt gtcgctgtca gcctcgtgcg
     3841 agccctggtc ggcgagcgca tcagccttgc tgccgaacgc tttgtctact tcgccgggtt
     3901 tgtgttcctg atggcgttgc tcgcgtgggt gacgctgttc gacacgggga tcctccaacg
     3961 gacctgacgg cccagccgcg cgccgagatc cagcagccgg ccatccagga gaagcgcagg
     4021 gacgtcggtc tcgtag
//
LOCUS       DATLCO010000074         8238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1573437, whole genome shotgun sequence.
ACCESSION   DATLCO010000074 DATLCO010000000
VERSION     DATLCO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573437"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1045)
                     /locus_tag="VMZ33_01850"
     CDS             complement(74..1045)
                     /locus_tag="VMZ33_01850"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUR16004.1"
                     /translation="MQESSLTAKPGAEELEVHLERYRRELIAYCYRMLASPFEAEDAV
                     QETFLRAWRSYERFEGRAALRSWLYRIATNVCLDMLSGRERRATPMDLGPAREPVLEN
                     LNTLPEVTWIQPIPDPAEEAVARETIRLAFVAALQHLPPRQRAVLILCEVLRWKASEV
                     AELLETSVASVNSALQRARATLEESNVSAADTPSSVDAADAELLARYVEAFERYDIDA
                     LTSLIHEDATQSMPPFDLWLRGRADIFEWWFGPGIGCRGSRVIPTPAANGAPAFGQYK
                     PSESGPGYEPWALQVIEVSDGKIGEFTFFLDTDTLFPLFGLPPRLDA"
     gene            complement(1100..1459)
                     /locus_tag="VMZ33_01855"
     CDS             complement(1100..1459)
                     /locus_tag="VMZ33_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HUR16005.1"
                     /translation="MQGLLIRWAVLAGAFAVMAWFLSGVTVSGGAWGYVWVSALFGIV
                     NAIVGTVLRILTFPLTVLTLGLFSVIVNALLLELTDGLSGHLTIDEFWWTAIWAAIIL
                     SLASMVLDAAARSVLPD"
     gene            1780..2334
                     /locus_tag="VMZ33_01860"
     CDS             1780..2334
                     /locus_tag="VMZ33_01860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HUR16006.1"
                     /translation="MTTLENRSNTALLVIDVQQGVVQGNHERDAVVANVGSLVEKARR
                     EEVPVVWVQHSDDGLQRGSDDWQIVPELKPADAEPLIEKNYGDSFEDTNLETVLAELG
                     VGRLVVVGAQTDACIRSTLHGAFTRGYDATLVSDAHTTEDQTAWGAPPPDQVIAHTNL
                     YWQNHTAPGRTAGTIETNDVDFAG"
     gene            complement(2370..2681)
                     /locus_tag="VMZ33_01865"
     CDS             complement(2370..2681)
                     /locus_tag="VMZ33_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16007.1"
                     /translation="MTRRLVPGVVTVVALALGGCGVGVDARVEKYEGGDVGVSACHKT
                     GARITAAGTYPANAESGYDSADEVWKCMVTETSGAVDKCYVVHESKVDTIVRGVKCSD
                     A"
     gene            2881..3174
                     /locus_tag="VMZ33_01870"
     CDS             2881..3174
                     /locus_tag="VMZ33_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16008.1"
                     /translation="MNERVAEVGDKLGLDTVSAICECAAAGCVERFELSRSEYEGVRS
                     SSDRFAVVPGHEEPEVERVIERREGYLILEKVGVGAAVADREDPRSDPPRKED"
     gene            3176..3547
                     /locus_tag="VMZ33_01875"
     CDS             3176..3547
                     /locus_tag="VMZ33_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16009.1"
                     /translation="MDEPTADVGEWSAERVALNDATFRNANEGIEERAESLDVDPVPF
                     LCECADMTCTEVIRLSLKDYEEIRSDSTHFLNVPGHQVAAGPHGKVVAEREGYVIVQK
                     IGRAGEIVADLDERSAEERAS"
     gene            3495..3932
                     /locus_tag="VMZ33_01880"
     CDS             3495..3932
                     /locus_tag="VMZ33_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16010.1"
                     /translation="MRSSLTWTKEAQKSGPARYPVSYALIEGKEGAVVSGDIDPTQQS
                     QIRAAKNEVLFREVNERIQFLRPPSTFVEFICECAKADCDEPISLTHQEYATLRSVPY
                     RFAVKPGHATPSIELVVVEQADRYDTIEKVGVGRDLVEAVDPR"
     gene            5067..5513
                     /locus_tag="VMZ33_01885"
     CDS             5067..5513
                     /locus_tag="VMZ33_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16011.1"
                     /translation="MGQGETGFAGTIRAAREAAGKDPGELAAALEMSYESYRDIEWYD
                     DEITSVVSYRDAVTLAGLLELDLRGMFGADDTVIAFADLAAALRLRLGGTSLEQLEDE
                     LGWELAGALADPTTFAEFSLDGLADVAGPLGLDWRHLLPVPRPANE"
     gene            5642..6391
                     /locus_tag="VMZ33_01890"
     CDS             5642..6391
                     /locus_tag="VMZ33_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HUR16012.1"
                     /translation="MRADRMGIEVVVAHSERATLRAGDVFLKIDSDQTRTDVEVAAME
                     MVPVPTPEILWREPPVLALAALPGTALGRLHEPSTASSAAWAAAGAAVRALHDAPLPP
                     WPGRSVDELASRLDGECQWLVANDVLPVDVVRRNRRLAEAALRRWTPMFIHGDLQLDH
                     VFVDGDEVTGIIDWSEASQGDALYDLAILTLAHEEHLGDVLAGYGTDVDRDLIRAWWS
                     WRCLVVVRWLFEHGFGPIETYPEFAVLTSGA"
     gene            6546..6749
                     /locus_tag="VMZ33_01895"
     CDS             6546..6749
                     /locus_tag="VMZ33_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16013.1"
                     /translation="MFANVTGRGYVQTAPDVHDNATIVNGFSDLIVEIWGDAGRHARS
                     APGQGPSPLNVPIIIDAIVAVSE"
     gene            6815..7252
                     /locus_tag="VMZ33_01900"
     CDS             6815..7252
                     /locus_tag="VMZ33_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR16014.1"
                     /translation="MREVVEADLPVFYEHQRDREAAAMAAFPPRERDTFMAHWAKTLA
                     SDSALTWTVVSDGAVAGNIGCWEDDGRRLVGYWIGREFWGRGLATQALAELLDVVDAR
                     PLHAYVAKRNVASIRVLEKCGFVKVGEHAGDDGIEELLLELRG"
     gene            7344..7784
                     /locus_tag="VMZ33_01905"
     CDS             7344..7784
                     /locus_tag="VMZ33_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16015.1"
                     /translation="MNFAPTSASAREAHASRQLAQWSQAFLRGEGGNLSVANPLHERD
                     NDVYVLAEVGLDDVYPISGADEDFDWPIPPAEFERRIKTMMQMFDDGWDAPPLFVHTH
                     TLRLVDGNHRREALLRLGRRTYWAVMWFRRPPVNGRGQPVSLDA"
     gene            8057..>8238
                     /locus_tag="VMZ33_01910"
     CDS             8057..>8238
                     /locus_tag="VMZ33_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16016.1"
                     /translation="MTDNNVGVPPEDAECRVMVCDDQPGYRKIVSAVLDLEPGLVVVG
                     EAANGQEAVDLVEELRP"
BASE COUNT         1487 a         2651 c         2750 g         1350 t
ORIGIN      
        1 tccggctgcg gaatgcttcc gcggagctgg tcgagctcct ggacttcatg ggcctgaccg
       61 aggtcttgac ggattaggcg tcgagccggg gcggcaggcc gaacagggga aagagcgtgt
      121 cggtgtcgag gaagaacgtg aactcgccga tcttgccgtc ggacacctcg atcacctgta
      181 gcgcccacgg ctcgtagccc gggccgctct cactcggctt gtactggccg aaagccggcg
      241 cgccgttggc ggccggggtc gggatcaccc gggacccgcg gcagccgatc ccggggccga
      301 accaccactc gaagatgtcc gcgcgacccc tgagccagag atcgaaggga ggcatcgact
      361 gcgtcgcgtc ctcgtggatc agcgacgtca gcgcatcgat gtcgtaccgc tcgaaggcct
      421 cgacgtagcg ggcgagcagc tccgcatccg ccgcgtcgac cgaggaaggc gtatccgcgg
      481 cgctcacgtt gctctcctcc agcgtcgccc gcgcacgttg cagcgcgctg ttgaccgaag
      541 cgacgctcgt ttccaggagc tccgcaacct ccgacgcctt ccagcgaagc acctcgcaga
      601 ggatcagcac ggctcgctgg cgcggcggca ggtgctgcag cgcggcgacg aaggccagcc
      661 ggatcgtctc tcgggccaca gcctcctcag ccgggtcggg gatcggttgg atccaggtca
      721 cctccgggag cgtgttcagg ttctcgagca cgggctcgcg agccggcccg agatccatcg
      781 gggtcgcccg tcgctcgcgg ccgctcagca tgtccaggca gacattggtg gcgatccggt
      841 agagccagga acggagcgcg gcgcggccct cgaaccgctc gtaggagcgc caggcccgga
      901 gaaaggtttc ctgcacggcg tcctccgcct cgaacgggga ggcgagcatg cggtagcagt
      961 acgcgatcag ctcgcgccgg taccgctcga ggtgcacctc cagctcctct gcaccgggct
     1021 tggccgtgag cgacgattcc tgcacgcacg tcatcgtagc ctccgactcc cggagagccg
     1081 gaaactcatc gtccgcgttc tagtcaggca agaccgagcg cgcggccgcg tccaggacca
     1141 tcgaggcgag cgacaggatg atcgccgccc agatcgcggt ccaccagaac tcgtcgatcg
     1201 tgaggtgacc ggagagcccg tcggtgagct ccagcaggag cgcgttgacg atcaccgaga
     1261 acagtccgag tgtcagcacg gtgagcggaa acgtgaggat gcgcaggacg gttccaacga
     1321 tcgcgttgac gatgccgaag agggcggaga cccagacgta cccccaggcg cctccggaca
     1381 cggtgactcc cgacaaaaac cacgccatga ctgcaaaggc gcctgcgagg acggcccacc
     1441 ggatcagaag tccctgcaca ggcgggctag gatacacggc cgacagcggc gtttccgccg
     1501 cagcgcccgg gctggacttg ccctaagcct gccgagttgc gttcgcgctg acggcctggc
     1561 cgcaccgatc acagacacgc acggagacac cggagacgtc cgtcatcgaa ccgtagcggt
     1621 gaccaagcag aagacagagc aggcgcctca taggtcttct catcggtcgg tgccggtcac
     1681 ttcacgtagt cccccatcct gtgttcctcg cggtgcttga ctcggccggg gtgtatctat
     1741 ggttgagcca acgaaaacca gcttgtccga gggaggacga tgaccacact ggaaaacagg
     1801 tccaacaccg ccttgctcgt catcgacgtg cagcagggcg tggttcaggg aaaccacgag
     1861 cgcgacgcgg tggtcgcgaa cgtcggcagc ctcgtcgaga aggccaggcg ggaggaagtg
     1921 ccggtcgtct gggtgcagca ctccgacgat gggctccaga gaggaagcga cgactggcag
     1981 atcgtcccgg agctgaaacc ggctgacgcc gagcctctga tcgagaagaa ctacggcgac
     2041 tccttcgagg acacgaacct ggagacggtc ctggccgaac tcggtgtcgg gcggctcgtg
     2101 gtcgtcggcg cacagacgga cgcgtgcatt cgctcgacgc tgcacggcgc cttcacgcgc
     2161 ggctatgacg cgacgctggt cagcgacgcg cacacgacgg aggatcagac tgcgtggggc
     2221 gcgccgccac cggaccaggt catcgcgcac acgaacctct actggcagaa ccacacagcg
     2281 ccgggccgaa ccgccggaac gatcgagacc aacgatgtcg acttcgccgg ttagagcgga
     2341 cgtttgttgg cagaatcagg cagcgcctgt taggcatccg agcacttcac accgcggacg
     2401 atggtgtcga ccttagattc gtgcacgacg tagcacttgt ccacagcgcc ggacgtctcg
     2461 gtgaccatgc atttccacac ttcgtctgct gagtcgtagc cgctttccgc gttcgcggga
     2521 tacgtcccgg cggccgtgat ccttgcgccg gtcttgtggc aggcgctcac cccgacgtcg
     2581 ccgccttcgt acttctctac gcgcgcgtcc acgccgaccc cacatccgcc cagggcgagc
     2641 gcgacaaccg tgacgacacc tgggacgaga cgacgagtca tcgacctgac tataggaccg
     2701 gcgggctagc tctgttcttt cgggtgctca gtgggcgccg ttcttcacct tgatccaagc
     2761 gaacgcccta ccaatggcca acgcggctac ttgtgtgtca gagacctcct cgcggggcac
     2821 acatctcggg tgaagacgag ggaagagcgg ctggccgcga acgaagcgat ttttcgtgag
     2881 gtgaacgagc gagtcgctga ggtcggcgac aagctcgggc tggacaccgt cagcgcgatc
     2941 tgtgagtgcg cggccgcagg ctgcgtcgag cgcttcgagc tctcgcggag cgaatacgag
     3001 ggagttcgct cgtcaagtga tcgattcgcc gttgttcccg gccatgagga gccggaggta
     3061 gagagagtca tcgagcgccg cgaggggtac ttgatccttg agaaggtggg tgtcggggcc
     3121 gccgtcgctg atcgcgaaga cccccggagc gaccctccta ggaaggaaga ctgacgtgga
     3181 cgaaccaacg gctgacgtgg gcgaatggag tgcggagcga gtagccctga acgacgcaac
     3241 tttccgcaac gccaatgaag gtattgagga gagggccgaa tcgctggacg ttgatcccgt
     3301 tccgttcctc tgcgaatgcg ctgacatgac ctgtaccgag gtgatcagac tgagcttgaa
     3361 agactatgag gagatccggt ctgactcgac tcattttttg aacgtgccgg ggcatcaggt
     3421 tgcagcgggt ccgcacggaa aggttgtggc tgaacgagag ggttacgtga tcgtgcagaa
     3481 gatcggtcgg gccggtgaga tcgtcgctga cctggacgaa agaagcgcag aagagcgggc
     3541 cagctagata tcccgtctcg tacgcgctaa tagagggaaa ggaaggagcg gtcgtgagcg
     3601 gggatattga ccccacgcag cagagccaga ttcgggcggc gaagaatgag gtgctcttcc
     3661 gcgaggtaaa cgagcggatt cagtttctcc gccccccttc caccttcgtc gagttcatct
     3721 gtgaatgcgc gaaagcagac tgtgacgagc cgatctcgct gacccatcag gaatacgcga
     3781 ccctaagaag cgtcccctac cggttcgccg tcaagcccgg acatgccacg ccgtccatcg
     3841 aacttgtggt cgtcgagcag gctgaccggt acgacaccat cgaaaaggtc ggagtaggcc
     3901 gcgacctggt tgaggccgtc gacccacggt aggccctctg tccggacaag agaagctgct
     3961 cagagcgcgg cgcgaattcg ccgacggttt cagtgctcgc gagcttgccc ggccagtggg
     4021 agttcgtagc tctcgttgcc ggcatgtgct gcgatcagaa gggcgcgccc ctcttcgcgg
     4081 cgcctcgcgt attcagcgcg gctgagggtg atgcgtccct ggcaggcggc ctgcccacac
     4141 tcgcagaaga agtaggactc ccgaagattg cgcgacctgt gcaggcggtc gattgcgtca
     4201 ttgacgtctg ccatcatcac ccgctgggag ctagtacgca tcgcagcacc tccccaaaaa
     4261 cctcactggc tttcgccctc ttgtttgttg agggcccgac gagctcaggt gctgcgaagt
     4321 ctgctccatg attcatgtca cacgactgaa cagccgcgtg tgatctgccc acatctggcg
     4381 tccggaaaca gctcgtcgca agcgctgcgc cgccgagccg ctgggtgccc gcacccgaag
     4441 ctgctaggag aggtcgcatc cggtgacgag gcaggcggcg aggcgtagaa ggatggacgc
     4501 gacgaagatc gccggcacga ggacgccggc aaaggccgcg tagagcgcca cggtgcgagc
     4561 tccccgccga acgcgaacga gaccaacgcg gcaatcgtgg cggcgagacc gcaggccaca
     4621 cccgcatcgg ctttgacccc cctcccgctt gagagaggaa ggtgtcggcc gcggctgtcc
     4681 tagcgcgtat ccacgtgctg ctggcgagcg ttatcccggc gaggaacgca ccgatcgcga
     4741 tgcggaacaa cggatggtgg gggttgactt cgctcagaga aaatcgggtt gaggttccgc
     4801 gccgctgcgc gtggtgcgta cgcttctgcg tcaacggcga ctggatccac gggcgacgag
     4861 taggcgaccg cgcgagcctc ggatcggccg aggcgaccca cacgatctgc caggactgct
     4921 ggcgacggct gcggctcgaa gggaacagcg ttggctgaag acgacagcgc tcacccgccc
     4981 gagcctcgcc tcttcaatcg gggtgcggcc gggcggttct aaagccgagt cgccccgtcc
     5041 aacagcgaga atcaacgagt cacagcatgg gacaaggcga aaccggattc gcgggaacga
     5101 tacgggcggc gcgcgaggcc gcggggaaag atccgggcga gcttgcggcg gccctggaga
     5161 tgtcgtacga gtcgtaccgc gacatcgagt ggtacgacga cgagatcacc agcgtcgtct
     5221 cctaccgcga cgccgtcact ctcgcaggcc tcctcgagct cgaccttcgt ggcatgttcg
     5281 gtgccgacga cacggtcatt gcattcgcgg acctagccgc cgcccttcgt ctgcgcctgg
     5341 gcgggacttc gctcgagcag ctcgaggacg aacttggctg ggaacttgcg ggtgcattgg
     5401 ccgaccccac cacctttgcc gagttctcgc tggatggact tgccgacgtc gcgggcccct
     5461 tagggctcga ctggcggcat ctgttgcccg tgccgcgccc cgccaacgag taactcggct
     5521 cgcacaagtc actggtaccc gacaccggtg aggcccagtg ccggcgccct gggccaggtt
     5581 cgggaatgag gcttcagcgc aggatgaccg ctagcctcga ccgcgtgaca gaggtcagag
     5641 cgtgagagcc gatcggatgg ggatcgaggt cgtggtcgcg cacagcgagc gagcgaccct
     5701 gcgtgccggc gatgtgttcc tgaagatcga ttctgatcag acgcgcaccg acgtcgaggt
     5761 tgcggcgatg gaaatggtgc cggtcccgac gccggagatc ctgtggcggg agccgcctgt
     5821 gctcgcgctc gccgctcttc cgggcacggc gctcggccgc ctccacgagc cgtcgaccgc
     5881 ctcgtcggcc gcgtgggccg cggcgggtgc cgccgtccgg gcgctgcacg atgcgccgct
     5941 gccgccatgg cccggtcgga gcgtcgacga gctcgcatcg cgactcgacg gcgagtgcca
     6001 atggctcgtc gccaacgacg tccttcccgt cgatgtcgtc aggcgtaacc gccgcctcgc
     6061 cgaggctgcg ctcaggcgct ggacaccgat gttcatccac ggcgacctgc agctcgacca
     6121 tgtcttcgtc gacggtgacg aagtcaccgg catcatcgac tggtccgagg cgtcccaagg
     6181 cgacgcgctg tacgacctcg ccatcctcac gctcgcgcac gaggagcacc ttggcgacgt
     6241 cctcgccggc tacggcaccg acgtcgaccg cgacctcatc cgtgcctggt ggtcgtggcg
     6301 atgcctggtt gtggtccgct ggctgttcga gcacggcttc ggcccgatcg agacgtatcc
     6361 cgagttcgcc gtgttgacat caggggcctg aagcagcaca agccactggg ggcggtcggc
     6421 cttaccgttc tccctcacct gataccggca gaggcacacg gcgacgagcc acccatcccg
     6481 tgggccagcg tccgggagtg ccagttgagc tggctaggag gcattggcga tgtattcgcc
     6541 tggcagtgtt cgcgaatgtc actggacgcg gctacgttca gacggcgccc gacgttcacg
     6601 acaacgccac gatcgtcaac ggcttcagcg acctgatcgt cgaaatctgg ggcgacgcgg
     6661 gccgacacgc ccgctcggcg ccgggccagg gcccgtcccc gctcaacgtc ccaatcatca
     6721 tcgacgcgat cgtcgcggtt tcggaatagg acgcggcggg agcctcgcca atagtcgaac
     6781 gtaccgacgg cggtacgctc gttgcgtgaa cgtcttgcgc gaggtcgtcg aggcggatct
     6841 gccggtcttc tacgagcacc agcgcgaccg ggaagcggcg gcgatggccg cgttcccgcc
     6901 gcgcgaacgc gacacgttca tggcgcactg ggcaaagacc ctcgcgagcg actcggccct
     6961 aacttggacg gtcgtctccg acggcgcggt cgccggcaac atcggctgct gggaggacga
     7021 cgggcgcagg ctcgtcgggt actggatcgg gcgagagttc tggggccgcg ggctcgcgac
     7081 gcaggccctc gcggaactcc tcgacgtggt cgacgcgcgc ccgctgcacg catacgtggc
     7141 gaagagaaac gtcgcctcga tccgcgtgct cgagaagtgc gggttcgtca aggttggcga
     7201 gcacgcgggc gacgacggga tcgaggagct cctcctggag ctgcggggat gagtacagac
     7261 ctgagcttaa gctcaggagc agcgggccta gcttgccagt gttagggaat gaccctgact
     7321 ggcccgcgct ttatcctcgg gcagtgaact tcgctccgac ctcagcatcg gcacgcgaag
     7381 cgcatgcctc ccgtcagctc gcccagtggt ctcaggcgtt ccttcgtggc gaaggcggga
     7441 acctgagcgt tgccaatccg ctccacgagc gtgacaacga cgtctacgtc ctggcagagg
     7501 tgggcctcga cgacgtgtat cccatctcag gggccgatga ggacttcgac tggccgatcc
     7561 cgcctgccga gttcgagcga aggatcaaga ccatgatgca gatgttcgac gacggctggg
     7621 acgcaccacc gctgttcgtt cacacgcaca ccctccgcct cgtcgatggc aaccatcggc
     7681 gggaggccct gcttcgccta ggccgccgca cgtactgggc cgtgatgtgg ttccgccgtc
     7741 cgccggtcaa cggtcgaggc cagcccgtct ccctggacgc gtgactttgc ctacgtgacc
     7801 cccgacggcg gcgcgctaac ggcggttctc tcccgccatc gacatcacgg tcgtccgcgc
     7861 aactacagca gcttccccgt cggggccgcg cgaccttttt cctgagtcgt acgccctcca
     7921 cgccttgggc gtacgtgacc gttcggagac tacgagggtc aagaaatcat gatctccacg
     7981 gtagtgcggg ctgctcattc ctgagctcat ccgcgatcat ctgcacgaga cgcggtgcgc
     8041 aaatacgcgg aggagcttga ccgacaataa tgtcggggtg ccgcccgaag acgccgagtg
     8101 ccgtgtgatg gtctgcgacg accagcccgg ctaccgcaag atcgtcagcg ccgtgctcga
     8161 tctggagccc ggactggtcg ttgtcggcga ggcggcaaat gggcaggaag cggtcgacct
     8221 cgtggaggag ctacggcc
//
LOCUS       DATLCO010000075        10735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3810496, whole genome shotgun sequence.
ACCESSION   DATLCO010000075 DATLCO010000000
VERSION     DATLCO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3810496"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VMZ33_01915"
     CDS             <1..262
                     /locus_tag="VMZ33_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HUR16017.1"
                     /translation="VDVALSSRDGVAALSVSDHGPGIDAEHAAQIFDRFYRVDSGRSR
                     ERGGAGLGLAIASSVASAHGGTIEYSDTPGGGATFTLTLPRA"
     gene            312..683
                     /locus_tag="VMZ33_01920"
     CDS             312..683
                     /locus_tag="VMZ33_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16018.1"
                     /translation="MPPRREEFGNHPQRETRQIREERGILSQDQPVVARVCEQQQEVV
                     DWVVAPLQVQPALECLGVVETHFQLDGCTQAESRDHRVSGTLLEGALRRLRKRNLGVE
                     GERRAHDVKHSGQTLYRRGIS"
     gene            complement(1283..1918)
                     /locus_tag="VMZ33_01925"
     CDS             complement(1283..1918)
                     /locus_tag="VMZ33_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16019.1"
                     /translation="MKGLIWLPVAGFLLVAGATVAAAAPGVADRAVSVFDADGSDTGP
                     APTTGEVRFGHEGGLLDEVLTELVDAGTITQAQSDAITEALTTKAEARHAEFEDQRDA
                     WQATAEQIRTFLEDGVISADEIAQLPADNPFSELESILDDGQITLQELESVGPFGGRF
                     LGGPGGEFDGRGPGGHGHGRGPRGPGGPGMWVPAPDEANAPSDASPEPSAS"
     gene            complement(2038..2679)
                     /locus_tag="VMZ33_01930"
     CDS             complement(2038..2679)
                     /locus_tag="VMZ33_01930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR16020.1"
                     /translation="MDDITSPAIEEYAAEHTSPEPPLLVELAHATREFSTASGMMVGR
                     LEGRFLKMLVAAVGAQRVLEIGTFTGYSALSMAEALPPLGRLITCELSEEHAAMARRF
                     IGRSAYSDMIEVRVGPAIETIRTLEGPFDFVFIDADKPSYLDYYESSLPLLSERGLIA
                     VDNVFQAGTVLDPNTDKQNAVALRRFNDHVAADPRVECAMVPIRDGVTLIRKV"
     gene            3193..4359
                     /locus_tag="VMZ33_01935"
     CDS             3193..4359
                     /locus_tag="VMZ33_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR16021.1"
                     /translation="MSIIASWTGAEEDSFRAMIQPWVDENETEVIYAGTRDMQALLSQ
                     AISAGGTGLPDVAGIPGPGLAREWYAAGALKSLDFIDFDTYSGATPPGMAELGQAEDG
                     VLVGIFTKAAVKGLIWYNVNNYDGTVPATFDELNNMDPAPAGAIWCNAIESGAASGWA
                     FTDWIEDIVIRQSGPEVYDSWVAGETLWSSPEIRSAFELLGDVVGKSHGGGTYINATN
                     FGDVGNEMFTDPAGCKFVHQASFISDFFVNQAGAAPEDFDFFVMPDIDTAYAGALTGG
                     GDMFGMFNDTPQARSLIQYLTTPEAQAIWAARGGYIAANTNVAADVYLSDSDRKAAEA
                     LQNASAFRFDGSDSMPGEMNTAFFSAMVELATDPANIDSILQELDTVQASAYGE"
     gene            4433..5419
                     /locus_tag="VMZ33_01940"
     CDS             4433..5419
                     /locus_tag="VMZ33_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HUR16022.1"
                     /translation="MDKLISAVIAIATVPIVLVGYIVLAEWAVKRFPPKTADRLRPIL
                     WVFPAVAMLFVFLVYPMIVTVWFSFWNFDPAGVNFTTFAGLENYATFFTNNDTLTALR
                     NNGLWVVLLPLLAVGIGLVIAVLVDRVRYESVVKSVIFLPMAISFVAATVIWKLVYDF
                     NPRIGILNAGVDALGGGPIAFIQDSPAWPAPLNTVMLVLVGTWMLTGFAMVILSAGLK
                     AIPSELLEAARVDGADEWYVFRKIVFPLLLPTIAVITTTVIIFGLKTFDVVYTMTSGN
                     FDTEVIANRMYKELAVFHEPGRASATAIILLTAIVPVMIYNIRRFREQEAQR"
     gene            5416..6330
                     /locus_tag="VMZ33_01945"
     CDS             5416..6330
                     /locus_tag="VMZ33_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HUR16023.1"
                     /translation="MTQAQAPVVVGEQLIATRRRRRFKSNPLIHISLFILMAIWALPA
                     IGLLVSSFREARDISRSGWWTALYTPSNFTLDNYQAVLGRSGLTESFINSFVITVPAT
                     VIVVMIAAFAAYAFAWMNFPGRDWLFVLLVGLLVIPLQVTLVPVLRLFGTFTDVTGVP
                     IHGTFLALWLAHTGYGLPFAIYLLRNYFGGLPKEVFESAAIDGASPVTAFFRLALPMS
                     VPVLASLIIFQFLWVWNDLLVALVYLGVSPDLFPLSVVLANLTTSRGGDWQFLTAAAF
                     VSMVVPIVIFLSLQRYFVRGITGGAVKG"
     gene            6532..7569
                     /locus_tag="VMZ33_01950"
     CDS             6532..7569
                     /locus_tag="VMZ33_01950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HUR16024.1"
                     /translation="MGLRAEIREQPAALARLLDHSASVMGEIRTAVHRQPPKFVVIAA
                     RGTSDNAALYAQYLFAVRNGIITALATPSTITLYGAQPQMAEALVIGISQSGASPDIV
                     AVIDEARRQGALTIALTNAPESALAQAAAITVDIQAGPERAIAATKTYTTELLAVALL
                     STALKPATAQESADLCSVPEWVDEALTVEPLAQTLASGHAQRDHSIVLGRGFAYATAR
                     EWALKLQELAQVSAFPFSAADFEHGPLALAEPGLPVLVVAPAGPPLDAQRHLLERLKA
                     ELGARLLVVSNDDDALRIDEGLPIPRGVSQWLSPLVEIIPAQLYTYYLTIARGLDPEA
                     PRTITKVTETH"
     gene            complement(7931..9286)
                     /locus_tag="VMZ33_01955"
     CDS             complement(7931..9286)
                     /locus_tag="VMZ33_01955"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HUR16025.1"
                     /translation="MIEEHSAREVGLRFPRAFVWGYAASAYQIEGAAAQDGRGRSIWD
                     TFARQPGAVVNAETGDVACDHYHRYREDVALMAEHGARAYRFSISWSRVLPAGVGQVN
                     RKGIDFYQRLVDSLLEAGIAPTVSLFHWDLPQALQDRGGFANPEVIGWFTDYTALMAN
                     ELGDRVRDWMTFNEPAVYAFLGHADGIHAPGIRHWPTAIRVADNQFRAHAAAGDVIRS
                     AVASARVGVAFDVNRIAPATDSDLDVRAAAEASVTRDAWFLDPLFGRGYPTAGKEAHA
                     AAGHLDGVELDTPPDGKLDYLGLNYYRQDNVWARSDEPFDWEVRTPVGVETTGMGWHV
                     QPRGLTDTLLDLTREYSPREIVITENGAAYDDKVDPDGQVRDANRISYLARHLGAVAD
                     ARDGGAPVTGYFVWSMMDNYEWSLGYGKRFGVFRVDFDTLARTPKESARWYRDVTEAG
                     R"
     gene            complement(9312..>10735)
                     /locus_tag="VMZ33_01960"
     CDS             complement(9312..>10735)
                     /locus_tag="VMZ33_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HUR16026.1"
                     /translation="RDIWALKISDNVATDEDEPEVLAECGMHAREHITVEMCLYMVRL
                     LTANYGQPGATGDRVTNIVNTREIWIIPTLNPDGAEYNISDGVFHNWRKNRQPNPGSS
                     AVGIDLNRNFSFMWGCCGGSSDKPGSGTYRGRYAFEAVEARVLRDFVLSRRVNGRQQL
                     RSVLNWHAYGARIYWPYGYTKTDVPSTMTVDDHEAFVALGHNMAALNDYVAHQGSDSY
                     IYDGDFPAWGYGNQRVFVFTFEMYPPFGCNGCGGFKPPDELIERETTRNRTAVLYLLE
                     QSDCPWRSAGLGWSNCGPLYDDFEVGRGWRINPYGTDTATRGAFARGIPQSSSDASGV
                     KQRAGAASGRMAMATGLTRGLSAAANDLDGGTTSAQSPALNLDTGAWTLRFAYSFGHN
                     TAATKADGVRFSVRDSTGLVTPVWTTTATAGVNRNAVWQTVSVPVDAWAGQAITLLVE
                     ATDSDADSLIEVAIDDVRVFRTP"
BASE COUNT         1834 a         3470 c         3454 g         1977 t
ORIGIN      
        1 cgtggacgtg gctttgtcgt cgcgtgatgg cgttgcggcg ttgagcgtct ccgaccacgg
       61 gccgggtatc gacgccgagc acgccgccca gatcttcgac cggttctacc gcgtggactc
      121 gggccgttcg cgcgagcgag gcggcgccgg gctggggctc gccattgcgt cgtccgtcgc
      181 cagcgcacac ggcgggacga tcgagtactc agacacgccc ggcgggggcg ccactttcac
      241 gctgaccctg ccgcgggctt aggcgcggcg cggtgggccg gcgacgggac cagcgcgagc
      301 gcactcggcg gatgccgccc cgccgcgagg agtttggcaa tcacccgcag cgggagacga
      361 ggcagatcag ggaagagcgc gggatactca gccaggacca gccggttgtg gcccgtgtct
      421 gcgagcagca gcaggaagtt gtcgactggg tcgtcgcgcc gcttcaagtc cagcctgcgc
      481 tcgagtgctt gggcgtcgtg gagacgcatt tccaactcga cggctgtacg caggccgaat
      541 ccagagatca ccgcgtctca ggcacgctgc tcgaaggggc gctccggcgt ttgcggaagc
      601 gcaacctcgg tgtggaaggt gaacggcggg cgcacgacgt caagcacagc ggccagacgc
      661 tttatcgacg cggcatctcg tagaggctcc gcaccgggat atgctttgat cggaagatcg
      721 agccccagca cggcagcgat ttggcaggcg cgaaggatcg aaagcgaagg gctttttcaa
      781 ccctctattt gggtatatcg agcacgtgag atcccgacag cttccgcgac ccgtgcttgc
      841 gaaagaccga ccgtcagccg cttgtctcgc agctcgtcgc caaggacggc cagggcccgc
      901 tgcccgcgtc gggtaccgcg atcgaacggc cgctctgtat tcgccacgag ctgaaggtag
      961 gcgcacgcac ttcacgccga cttaccgggt gttcacacca cgaaccaaac ggccatggct
     1021 gacgcttttt cacgttgaaa tggccgcgaa cgtaccgtct cgcgaacatt tggatgccta
     1081 caccccttcg atgttcacct gctgaacatc cagcccgaaa ggtccttaat cggttcgtac
     1141 tgtgaacgtc ggctgcggcg gcgtgttggc ggggaacgcg gcgaggccgg aggtcctggt
     1201 ggacctccgg cgtcgcacag gggtcgccag ccgggtagcc gagagggaag ctccccagtg
     1261 gcgggtatac gaatgggttt gcctagctgg cgcttggctc gggcgaggcg tcagacggcg
     1321 cattggcctc gtccggggcc gggacccaca tgccggggcc gccggggcca cgagggccac
     1381 gtccgtggcc gtgaccaccg gggccgcgac catcgaactc gccgccgggg ccgccgagga
     1441 accggccacc gaagggacca actgactcaa gctcctggag cgtgatctgc ccgtcatcga
     1501 ggatggactc aagctcagag aaagggttgt ccgccggcag ctgggcaatc tcgtcggcag
     1561 agatcacgcc gtcctcgagg aaggtcctga tttgctcggc cgtggcctgc catgcgtcgc
     1621 gctgatcctc gaactcggcg tgcctcgcct cggcttttgt ggtgagggcc tcggtgatcg
     1681 cgtcggactg ggcctgggtg atcgtgcccg cgtcgacaag ctcggtcagg acctcgtcga
     1741 ggaggccgcc ctcgtgcccg aagcgcacct cgcccgtggt cggagcgggt cctgtgtcgg
     1801 aaccgtccgc gtcgaacacg ctgactgcgc gatcggccac ccccggcgcc gccgcggcga
     1861 ccgtggctcc ggcaacgagc aggaaccctg ccaccggcag ccagatcagt cccttcattg
     1921 tgtgtgtacc tccaggttgt gttcgaaccc cggaccaaat ggtcgagtgg gtcgcttggc
     1981 cccgtctgtc ggcccgctgg aggtttgctg tgaatgccgc ccttgcagcg gcatggatca
     2041 gaccttccga atgagagtca caccgtcccg gatgggcacc atcgcgcact ccacacgcgg
     2101 atcggcagcc acgtggtcgt tgaagcgacg tagcgccacg gcgttctgct tgtccgtgtt
     2161 tggatcgagc accgtgccgg cctgaaaaac gttgtcgacc gcgatgagtc cacgctccga
     2221 cagcagcggc aaggatgact cgtagtagtc gagatacgac ggcttgtcgg cgtcgatgaa
     2281 gacgaagtcg aatggacctt cgagcgttcg aatggtctca atcgccggcc cgacacgcac
     2341 ctcgatcatg tccgagtacg ccgaccgccc gatgaaacgg cgtgccatcg ccgcgtgctc
     2401 ctcgctcagc tcgcaggtga tcaaacgtcc taacggtggc agcgcctcag ccatagagag
     2461 tgccgagtag ccggtgaagg tgccgatttc aagcactctt tgcgctccaa cggcggcgac
     2521 cagcatcttc aggaagcggc cttcgaggcg gccgaccatc atgccggagg cggtcgagaa
     2581 ctcacgcgtt gcgtgcgcca gctcgaccag cagcgggggc tctggcgacg tgtgctcggc
     2641 agcgtattcc tcgatggctg gactcgtgat gtcgtccatg gccgaatatt cgcacggcac
     2701 agcccatcct tcttgcggcg gacgatccgt cgcaggccca atcaaatgat tgcgttgcgc
     2761 gcaccccgtc gttaggcttc gattgatact gcggttggga ggccgctgag ggcgctcgtg
     2821 cccgtgagta ttggcgccgt tgacagtcgc cccgggaacc ccgcggcaac gaacggcgga
     2881 cgagacatca caaatccggc gagcgcccag agcagccgga gcataagagg aggcgagaca
     2941 gtgcaactgt ctaggactgg tatttccagg ttgttcgccg cgttgatcgc ggtgctcatc
     3001 gtcgcggcgg catgtggtcc ggccgcaacc acgtccactc gtgggccagc tactggcgct
     3061 gcaccgactg aggccgcacc gagtgaggcc gcaccaaccg aggccgcacc gagtgaggct
     3121 ccaccgagtg aggctgcacc gagtgaggct gcaccgaccg atccaccgcc aggtggcccc
     3181 ctcggcggaa ccgtgagcat catcgcctcg tggactggag ccgaggagga ctcgttccgc
     3241 gccatgatcc agccgtgggt ggacgagaac gagacagagg tcatctatgc cgggacgcgc
     3301 gacatgcaag cgctgctcag tcaggccatc tcggccggcg gcacgggcct gcccgacgtc
     3361 gctggcatcc ccggccccgg acttgcccgc gagtggtatg cggcgggtgc gctcaagtcg
     3421 ctcgatttca tcgacttcga tacgtactcg ggcgcgaccc ctccaggcat ggccgaactc
     3481 ggccaggctg aggatggcgt cctggtcggc atcttcacca aggcggctgt caagggcctc
     3541 atttggtaca acgtcaacaa ttacgacggg accgttccag ccacgttcga cgagctgaac
     3601 aacatggatc ccgccccagc tggggcaatc tggtgcaacg caattgagtc aggcgctgcg
     3661 tcgggctggg cgttcaccga ctggatcgag gacattgtca ttcgccagtc cgggcctgag
     3721 gtctacgaca gctgggtcgc cggcgagacg ctctggtcct cgcctgaaat tcgctcggcg
     3781 tttgagctgc tgggtgacgt cgtcggcaag agccacggcg gtgggacgta catcaacgcc
     3841 acaaacttcg gtgacgtcgg caatgaaatg ttcaccgatc cggcaggttg caagtttgtc
     3901 catcaggcga gcttcatcag cgacttcttc gtcaaccagg cgggcgccgc gccggaggat
     3961 ttcgacttct tcgtaatgcc tgatatcgat accgcctatg ccggagcgct gactggcggc
     4021 ggcgacatgt ttggcatgtt caatgacacg ccccaggcgc gctcgttgat ccagtatctg
     4081 acgacgcctg aggcacaggc catctgggcg gcccgcggcg gttacatcgc agcgaacaca
     4141 aacgtggccg ccgacgttta tctcagcgac tcggaccgca aggcggctga ggcgttgcag
     4201 aacgcctccg cgttccgttt cgacgggtcc gattcgatgc caggcgagat gaacacggcg
     4261 ttcttcagcg caatggttga acttgccact gatccagcca acatcgactc cattcttcaa
     4321 gagctcgaca ccgttcaggc atctgcctac ggcgagtagc cacagcgagc gacgacgggc
     4381 cggcgacact cagtcgccgg cccgttcttt ctagcgttca tccggagagg gcatggacaa
     4441 gctcatcagc gcggtcatcg caatcgcgac ggttccgatc gtgctcgtcg gctacatcgt
     4501 gctcgccgaa tgggccgtca agcgattccc gccgaagact gccgatcggt tgaggcccat
     4561 cctgtgggtc tttcccgccg tggcgatgct tttcgtcttc ttggtctacc cgatgatcgt
     4621 gacggtgtgg ttcagctttt ggaacttcga tcccgctgga gtgaacttca ccaccttcgc
     4681 cggtctcgag aactacgcca cgttctttac caacaacgac acgctcacgg ccctgcgcaa
     4741 caacggtctg tgggtcgtac ttctgccatt actggcggtc gggatcgggc tggtcatcgc
     4801 cgtacttgtt gatcgcgtcc gttacgagag cgtggtcaag tcggtcatct tcttgccgat
     4861 ggcgatcagt ttcgttgctg cgaccgtgat ctggaagctc gtatacgact tcaacccacg
     4921 gatcgggatc ctcaatgccg gagttgatgc gctgggtggc gggccgatcg ccttcataca
     4981 ggactcgcct gcatggcctg cgccactgaa tacggtgatg ctggtactcg tcggcacctg
     5041 gatgctcacg ggtttcgcga tggtcatcct gtcggcgggc ctgaaggcga tcccgtccga
     5101 actgctagag gcggcgcgcg ttgacggggc cgacgagtgg tatgtcttcc ggaaaattgt
     5161 gttcccgctg ttgctaccca cgatcgcggt gatcacgacg acggtcatca tcttcggatt
     5221 gaagaccttc gacgtcgtgt acacgatgac tagcggaaat ttcgataccg aggtgatcgc
     5281 caaccggatg tacaaggagt tggcggtttt ccatgagcct ggccgagcaa gcgccaccgc
     5341 catcattctg ctgaccgcaa tcgtgcccgt aatgatctac aacatccggc gctttcgtga
     5401 gcaggaggca cagcgatgac ccaggcacag gcaccggtcg tcgttggaga acagttgatc
     5461 gccacgcgcc gccgacggcg cttcaagtcg aaccctctga tacacatatc gctattcata
     5521 ctcatggcga tctgggcgct gccggcgatt ggactgttgg tcagctcgtt tcgcgaggct
     5581 cgcgatatca gtcggagcgg ctggtggact gccctctaca cgccgtccaa cttcaccctg
     5641 gacaattatc aggctgtgct tggccggtcg gggctaactg aatcattcat caacagtttc
     5701 gtcatcaccg tcccggcgac agtgatcgtg gtcatgattg ccgcctttgc ggcatacgcc
     5761 ttcgcctgga tgaatttccc tggacgcgac tggctgttcg tcctcctcgt cggcctgttg
     5821 gtcatacccc tccaggtaac actggtgccg gtgctccgcc tgttcgggac cttcaccgat
     5881 gtgaccggag tgcccattca tggaacgttt ctcgctcttt ggctggctca cacaggctat
     5941 ggactgccct ttgctatcta cctgctgcgt aactacttcg gcggcctgcc gaaggaggtc
     6001 ttcgagtccg cagccatcga cggcgcgagc cccgtcacgg cgtttttccg cctggccctg
     6061 ccgatgagcg tcccggtact cgcctcgctc atcatcttcc agttcctatg ggtctggaat
     6121 gacctactcg tggcccttgt ctatctcggt gtgtcgccag atctgttccc actttcggta
     6181 gttctggcaa atctgaccac gtcgcgcggc ggcgactggc aattcctgac tgccgcggca
     6241 ttcgtttcga tggtcgttcc gatcgtcatc ttcctgtcat tgcagcgcta cttcgtgcgc
     6301 gggatcacag gcggcgcggt caagggttaa gcgcgaagcc gtggcacgac cagctctccc
     6361 cggctgttcg cggaatggat acagctactc gacgaaatgc gtgcgcgtgc acacggccgc
     6421 gccgcctgaa cctcgggggc gcatgagctc ggtcgtgatc atcgcccgac gatgtggcct
     6481 ctttcccaat cacgtcgctc gcgcagaggt tgtcgccgcg tcaggcacgc aatggggctg
     6541 agagccgaga tccgcgagca gcccgctgct cttgcgcgcc tcctcgatca ttcggccagc
     6601 gtcatgggcg agattcggac ggccgtccat aggcagccac cgaagttcgt ggtcatcgcc
     6661 gcaagaggga cctccgacaa cgccgccctc tacgcccagt acctgttcgc cgtccgcaac
     6721 gggatcatca ccgccttggc cacgccgtcg actatcaccc tgtacggcgc gcagccccag
     6781 atggccgagg cgctggtcat cggcatctcg caatcgggcg cctcgccaga catcgttgcc
     6841 gtcatcgacg aggcgcggcg gcaaggtgcg ctgaccattg ccctgaccaa tgcgccggag
     6901 tcggcgctcg cacaggccgc cgcgatcacg gtcgatatcc aggccggccc cgagcgggcg
     6961 atcgccgcca ccaagaccta cacgaccgag ctgctggcgg ttgcgctgct gtctacggcg
     7021 ctcaagccgg cgacggccca ggaatcggcc gacctctgct ccgtccccga gtgggtcgat
     7081 gaggccctga cggttgagcc gctcgcccaa acgctcgcgt cgggccatgc ccaacgcgac
     7141 cactccatcg tcctcggccg aggcttcgcc tatgcgacgg cgcgcgagtg ggcgctgaag
     7201 ctgcaggagc tggcgcaggt gtcggccttc ccgttctcgg cggctgattt cgagcacgga
     7261 ccgctggccc tggccgagcc tggcctgccg gtgttggtgg tggccccggc tggtccaccg
     7321 ctcgacgccc agcgtcactt gctggagcgg ttgaaggccg agctcggagc gcggctgctg
     7381 gtcgtatcga acgacgacga tgctctgcgt atcgacgaag gcttgccgat cccgcggggc
     7441 gtcagccagt ggctgtcacc gctcgtggag atcattccgg cgcagcttta cacgtactac
     7501 ctgacgatcg cgcgcggcct cgatcctgag gcgccgcgaa ccatcaccaa ggtgaccgaa
     7561 acccactgag gacggcccca aacaccagaa gacccacact ccgggaacga gtgtgggtca
     7621 cgcgtcaagg gtagggcgat ctaagggtgg gcaactgtca tgagtcgctc gcgaccccgg
     7681 atcacccggt tctgccggcc cgcgtatcgc tgctgatgtt gcccgacctg ggcacggaag
     7741 cgaggttcgc tgcgccacgc gacagatccg cggttgctgg caggcctttg gaagccacgg
     7801 ctaccaacgg cctccggtct ggccgatcta agcggcgagg gcggtctttc gacactgggc
     7861 gctacctctg ctttcccgtt gatcttacgc ccaagctcca agaaggaacg cctttcaggg
     7921 acataacaga tcaccgaccg gcctcggtca cgtcgcggta ccaacgggcg ctctccttcg
     7981 gtgtccgagc gagggtgtcg aagtcgacgc ggaagacccc gaagcgtttg ccgtagccga
     8041 ggctccactc gtagttgtcc atcatcgacc agacgaagta ccccgtgacc ggcgcgccgc
     8101 cgtcacgcgc atccgctacc gcacccaggt ggcgcgccag gtagctgatt cgattggcgt
     8161 cgcgcacctg gccatcggga tcaaccttgt cgtcgtaggc ggccccgttc tcggtgatca
     8221 cgatttcgcg cggcgaatac tcgcgcgtca ggtcgagcag cgtgtcagtg agcccgcgcg
     8281 gctgaacgtg ccagcccatt cctgtcgtct cgacacccac gggggtgcgg acctcccagt
     8341 cgaacggctc atcggaacgc gcccaaacgt tgtcctggcg gtagtagttg aggccgagat
     8401 agtcgagctt gccgtcgggc ggcgtgtcga gctcaacccc gtcgaggtgc ccggctgccg
     8461 catgcgcctc tttgccagcc gtcggatagc cccgcccaaa gagcgggtcc aggaaccatg
     8521 catcgcgcgt gacgctggcc tcggccgcgg ctcggacgtc gagatcggaa tcggtcgccg
     8581 gcgcgatgcg gttcacgtcg aaagccacgc cgacgcgcgc cgacgcaacg gcggagcgga
     8641 tcacgtctcc ggcggcggcg tgggcacgaa actggttgtc ggccacgcgg atggccgttg
     8701 gccagtggcg gatccccggg gcgtggatac cgtcggcgtg gcccaggaag gcatagacag
     8761 caggctcatt gaaggtcatc caatccctta cccgatcacc cagctcgttg gccatgagcg
     8821 ccgtgtagtc cgtgaaccag ccgattactt cggggttggc gaacccgcca cgatcctgga
     8881 gcgcctgggg caggtcccag tggaagagac tgacggtcgg ggcgatgccg gcctccagaa
     8941 gcgagtcgac caggcgctgg tagaaatcga tgcccttgcg gttgacctgc ccgacaccgg
     9001 cgggcagcac gcgcgaccag ctgatgctga agcggtaggc gcgggcgccg tgctccgcca
     9061 tcagcgctac gtcctcgcgg tagcggtgat agtggtcgca cgcgacgtcg ccggtctcgg
     9121 cgttgacgac cgcgccgggc tggcgggcga aggtgtccca tatcgaacgg ccgcggccat
     9181 cctgggccgc tgcgccctcg atctgataag cggaagccgc gtagccccac acgaaagcgc
     9241 gcgggaagcg aaggccgacc tcgcgtgccg agtgctcttc aatcacccgg ggattattca
     9301 cgccaaggac gtcagggggt gcggaagacc ctcacgtcgt cgatagcgac ctcgatcagg
     9361 ctgtcggcgt cagagtccgt tgcctcgacc aggagcgtga tcgcctggcc cgcccaggca
     9421 tcgaccggta cggacacggt ctgccacacg gcgttgcggt tgacgccggc cgtcgcggtt
     9481 gtagtccaaa cgggcgtgac gaggccggtt gagtcccgaa cgctgaagcg aacgccgtcg
     9541 gccttcgtgg ctgcggtgtt gtggccgaac gagtacgcga agcgcagcgt ccaggcgcca
     9601 gtgtcgaggt tcagggcggg ggattgagcg gaggtcgtgc cgccatcgag gtcgttcgcg
     9661 gcagcgctca gcccgcgggt caaacccgtc gccatggcca tgcgaccgct ggccgcgccc
     9721 gcacgctgct tgacgccgga cgcatcggac gagctttgtg gtatgccccg ggcaaatgct
     9781 ccgcgggtcg ccgtgtccgt cccatacggg ttgatgcgcc agccacgtcc cacctcaaaa
     9841 tcgtcgtaca gcggcccaca gttggaccag ccgaggccag ccgaacgcca cgggcagtcg
     9901 gactgctcca acaggtagag caccgccgtt cgattacggg tcgtctcccg ctcgatcagc
     9961 tcatcgggcg gcttgaagcc gccgcagcca ttgcagccga agggtggata catctcgaaa
    10021 gtgaacacga acacccgctg attgccgtag ccccacgccg ggaaatcgcc gtcgtagatg
    10081 tacgagtcgc tgccctggtg cgcgacatag tcgttgagcg cagccatgtt gtgaccaagc
    10141 gccacgaagg cctcgtgatc gtccacggtc atggtcgagg gaacgtcggt cttggtgtac
    10201 ccgtacggcc agtagatgcg cgcgccgtaa gcgtgccagt tgaggaccga cctgagctgc
    10261 tggcggccgt tcacacgccg gctcagcacg aagtcgcgca ggacacgtgc ctcaaccgcc
    10321 tcaaaggcgt accggccgcg atacgtgccc gacccgggtt tatctgacga ccccccgcaa
    10381 cagccccaca tgaagctgaa gttgcggttg aggtctatcc ccacggcgct cgagcctgga
    10441 ttcggctggc ggttcttacg ccagttgtgg aacaccccgt ctgagatgtt gtactcggca
    10501 ccgtcggggt taagtgtcgg gatgatccag atctcgcgcg tgttgacgat gttcgtgacg
    10561 cggtcaccgg ttgcccccgg ctgaccgtag ttggccgtca ggagccggac catgtacagg
    10621 cacatctcca ccgtgatgtg ctcccgtgcg tgcatcccgc actcggcgag gacctccggc
    10681 tcgtcctcgt cggttgcgac gttgtcgctg atcttgaggg cccatatgtc gcggc
//
LOCUS       DATLCO010000076         4769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9732167, whole genome shotgun sequence.
ACCESSION   DATLCO010000076 DATLCO010000000
VERSION     DATLCO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9732167"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VMZ33_01965"
     CDS             <1..582
                     /locus_tag="VMZ33_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HUR16027.1"
                     /translation="HSGDLEQAQADLAPIKGHGQPLADLIQVKDYVAQQTMLDATQPK
                     GMHYYWKSEFVPGLSDGLFAAYNAQFEGLKAPANQMVLFQVGGALNDHPEDDGAVGNR
                     DARFACVIQSMWPPDSDAGTANQQWVRNAWQAIRPYSTGGNYVNFQTFDETDERTAES
                     YRGNFRRLAAAKAKYDPDNVFRVNRNIRPDAKG"
     gene            793..3636
                     /locus_tag="VMZ33_01970"
     CDS             793..3636
                     /locus_tag="VMZ33_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUR16028.1"
                     /translation="MPALNPRTIVAASLAVLLTASIAIPAAAGQPPAAQWPDPTAEAG
                     VFPFPGGVYRTPPGVSFASDRLLVKFRDGASAVEKTGARQSINASLDRDVKLVPGLQV
                     LGLRGSRQSDVIAAVAALTGRADVEYAMPDFAYELAVEPDDPSYPGQWAPAVMGAPSA
                     WARSTGSNAVTVAVLDTGIDLDHPDLAANLVPGWNFVADTDNPDDDHGHGTHVAGIIG
                     AAGNNGVGVTGVNWSVSLMPLKVCDAYGSCYLSAEIAALDFAVAHGARIANASFGALN
                     NEYPPERDAIKAAGDAGLLYVAAAGNDETDTDTTPFYPADYPLANIISVGASAPDDQL
                     AYFSNYGATSVDLVAPGLDIVSTFPDGYTTMSGTSMAAPQVAGAAALVKALHPTWTVQ
                     QIRRQLIRSATPVSALAAKAVSCGRLNLDAATDPATVLKAALCVARRGSGLGTVSSSD
                     GAIACGATCAAFFAANSQVTLTATPDAGSAFGGWSGPCSGTGTCTLNLSAAASVGAVF
                     IDPDPQGGWLNRLLPPPAGRNPLAPGSSTGRWTSFYNVALSANGKVRAKTVFNAPNGW
                     CYYASSDTGGIFLERKTSSGWKADGVLTAPALGPEPGDRWMNCAGFGTMTELSGDGST
                     LLVGNDVFFDRCVAFVYRRIAGIWQLDGTLFPPGVTAQGSTEPGKCGVFTLEGAISHT
                     GDRVAVYAPQAYEGNYRARVNVFARGTSGWALERTIFPPVAVGCAGWPNDTGDRRLAM
                     SGDGASILIGDPYCSAGAGRVNHYTRSVTTWTKRPVIASPDPTGRWFGKSVAISADGL
                     TAALGLTYGVTHNGDLTSSWVFELSSGTWTPRTRLDPAVADQDGSFSCAVLVRAGQRI
                     VCTAVDAHGFNSEQGSLYVFNRPAVGWAAGSKRTRLFAPFGYSFERLGTSGLPWQPDV
                     AAREDGTLIDATITPMGLVQGTTRDRIGYEFRR"
     gene            3668..>4769
                     /locus_tag="VMZ33_01975"
     CDS             3668..>4769
                     /locus_tag="VMZ33_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR16029.1"
                     /translation="MPAEFIYTTYKLSRHYPPDRTVLEDISISFYPGAKIGVIGPNGS
                     GKSSLLRIMAGLDDGYNGDARLTPGFTVGYLSQEPQLDPTKNVKENVLDGVREVHGLI
                     EQYNAVMAKWSDPEADYEAVGAEQSALEDKINAVGAWDIERNVEIAMDALRCPPDDAS
                     VSTLSGGEKRRVALCRLLLQHPDLLLLDEPTNHLDAESVEWLERFLAEYSGTVVAVTH
                     DRYFLDNVAQWILELDRGRGIPFAGNYSSWLEQKLGRLAGEQKQVDARQRTLARELDW
                     VRMGAKARQAKGKARLTAYEKLLDEANDARGVQQRELEIAIPPGPRLGETVIDVKGLR
                     KGYGDRLLIEDLSFSLPRAGIVGIIGPNGAGKT"
BASE COUNT          836 a         1613 c         1535 g          785 t
ORIGIN      
        1 cactcgggcg atctcgagca ggcccaggct gacctggcac cgatcaaggg ccacggccag
       61 ccgctagcgg acctcatcca ggtcaaggac tacgtcgccc agcagacgat gctcgacgcc
      121 acgcaaccga agggcatgca ctactactgg aagtcggagt tcgtgcccgg cctgagcgac
      181 gggctgttcg cggcctacaa cgcccaattc gagggcctca aggcaccggc caaccagatg
      241 gtcctgtttc aggtgggcgg cgcgctaaac gaccatccgg aggatgacgg agcggtcggc
      301 aatcgcgacg cccggtttgc atgcgtgatc cagtcgatgt ggccgcccga cagcgacgcc
      361 ggtacggcca accagcagtg ggtgcgcaac gcctggcaag cgatcaggcc gtactcgacc
      421 ggcggcaact acgtcaactt ccagaccttt gatgagaccg acgagcgcac cgcggagtcg
      481 taccgcggga acttccggcg cctcgccgcc gctaaggcga aatacgaccc ggacaatgtc
      541 ttccgcgtca accggaacat ccgccccgac gccaagggct gaagtcgcgc gtcgtctgcg
      601 accctagctc gccgcggggc aagagagcgt gggatcgact cccccgctca gggttggtac
      661 gtaggcccgt ccgggtacct cagtgggtag caacgcgggt tgtaaaccaa ggccgtgagg
      721 tctgtactca gttcgagctc tccaggttgc gccgtaagcg caaactgagc acgtaacccg
      781 agggagaccg caatgcccgc gttgaaccca aggacgattg ttgcggcgtc gctcgccgta
      841 ctgctcacgg cttcgatcgc tatccccgct gcagccggcc agccgccggc cgcccagtgg
      901 cccgatccca cggcagaagc cggcgtcttt cctttcccgg gcggcgtgta tcggacgccc
      961 ccgggcgttt cgttcgcctc ggatcggctg ctggtcaagt tccgcgacgg cgcgtcggct
     1021 gtcgaaaaga ccggcgcccg ccagtccatc aacgcttctc tcgaccgcga cgtcaagctc
     1081 gtccccgggc ttcaggtact gggcctgcgc ggtagtcgcc aaagtgacgt gatcgctgcc
     1141 gtagcggccc tgaccgggcg cgcggacgtc gaatacgcga tgccggactt cgcctacgag
     1201 ttggcagttg agccggacga tccgagctat cccggtcagt gggcgcccgc tgtcatgggt
     1261 gcgccttcgg cctgggcacg atcaacaggc agcaacgcgg tgacggtcgc ggtgcttgac
     1321 acgggcattg acctcgatca ccccgacctg gcggcgaacc tcgtgccggg ctggaacttc
     1381 gtggccgata ccgacaaccc cgatgacgac catggacacg ggacgcacgt cgccgggatc
     1441 atcggcgccg ccggaaacaa cggtgtcggt gtcacgggtg tcaactggtc ggtcagcctg
     1501 atgccgctca aggtttgcga cgcctacggt tcgtgctact tgagcgccga gatcgccgcg
     1561 ctggactttg cagttgctca cggagcccgt atcgccaacg ccagcttcgg tgctctcaac
     1621 aacgaatacc cgcccgagcg cgacgcgatc aaagccgccg gtgatgccgg gctgctctac
     1681 gtcgcggccg caggcaacga cgagactgat accgacacga cgcccttcta cccggccgac
     1741 tacccgctgg ccaacatcat ctcggtcggc gcgtcggcac cggacgacca gctggcctat
     1801 ttctcgaact acggcgccac ctctgtcgac ctcgtggcgc ccggccttga catcgtgagc
     1861 accttcccgg acggctacac gacgatgagc ggcacttcca tggccgcgcc ccaggttgcc
     1921 ggtgcagcgg cgctcgtgaa agcactccac cccacctgga cggtgcagca gatccgccgg
     1981 caattgataa gaagcgccac tcccgtatcg gccctggcag ctaaagccgt cagctgtggc
     2041 cgactcaacc tcgatgccgc caccgacccg gccacggtcc tcaaggccgc gctgtgcgtc
     2101 gcgagacgag gttcgggact tggaacggtg tcctcgagcg acggggcgat cgcctgtgga
     2161 gcgacctgcg ccgctttctt cgcggcgaac tcccaggtca cgctaacggc cacccctgac
     2221 gccggatcgg ccttcggcgg ctggagcggt ccctgttcgg gcacgggaac gtgcacgctg
     2281 aacctctcag cggccgcaag cgttggcgct gtgttcattg atcctgaccc gcagggcggt
     2341 tggctgaacc gacttctgcc gccgccggcg ggtcgcaacc cactcgcacc cggctcatcg
     2401 accggtaggt ggacctcctt ctacaacgtg gccctctctg ccaacggcaa ggtgcgcgcc
     2461 aagacagttt tcaatgcacc caatggctgg tgctactacg ccagctcgga taccggcggc
     2521 atattcctgg agcgcaagac ttcgtccggc tggaaggccg atggcgttct gacggcgccg
     2581 gccctgggac cagagccggg cgaccgctgg atgaactgcg ctggtttcgg aaccatgacg
     2641 gaactctcgg gcgacggatc gacgctactc gtcggcaacg acgtcttctt tgatcgctgc
     2701 gtcgccttcg tttatcgacg catcgccggc atctggcagc tggacggcac cctgttcccg
     2761 cctggggtga ccgcgcaggg ctcgacggaa cccggcaagt gcggcgtgtt cacgctggaa
     2821 ggagccattt cgcacaccgg tgatcgcgtc gcggtctatg ccccgcaggc gtacgaaggc
     2881 aactatcgag cgcgcgtcaa cgtttttgct cgcggcactt ccggctgggc gctcgaaaga
     2941 accatcttcc cgccagtcgc ggtcgggtgc gccggctggc caaacgacac gggcgacagg
     3001 cgactcgcca tgtccggtga tggtgccagc attctgatcg gcgatccgta ttgttcagcc
     3061 ggcgcggggc gcgtaaacca ctacacgcgg tccgtgacca cgtggacgaa acgcccggtc
     3121 atagccagcc ccgacccgac aggccgctgg ttcggcaagt cagtcgccat ctccgcggat
     3181 gggctgactg cggccctggg cttgacctac ggggttaccc acaacggcga cctcacgtcc
     3241 agctgggtct tcgagctcag tagcggcaca tggacgccgc gcacacgcct cgacccagcc
     3301 gtggctgatc aggacggctc gttctcctgc gccgtcctgg tccgcgccgg ccagagaatc
     3361 gtctgcaccg cggtcgatgc gcacggattc aactcggagc aaggttcgct gtacgtattt
     3421 aatcggcctg ccgtcgggtg ggctgcagga tccaagcgaa cacggctgtt cgcgcccttc
     3481 gggtactcct tcgagcggct tggaacgagc ggcctgccgt ggcaacccga cgtggccgcg
     3541 cgcgaggacg gcacgctgat cgatgccacg atcacgccca tgggtctcgt gcagggcacc
     3601 acccgcgatc ggatcggcta cgaattccgt cgttaggacg gcgtcctccc ggtaaccttc
     3661 gaggactgtg cctgccgagt tcatctacac cacgtacaag ctgtcccgcc actacccgcc
     3721 cgatcgaacg gtgctggagg acatctccat ctcgttctac ccgggcgcca agatcggcgt
     3781 catcgggccc aacggctcgg gcaagtccag cctccttcgg atcatggccg ggctcgatga
     3841 cggctacaac ggcgacgcgc ggctgacgcc cgggttcaca gtcggctacc tgagccagga
     3901 gccgcagctg gacccgacca agaacgtcaa ggagaacgtc ctcgacggcg tgcgcgaggt
     3961 ccacggcctg atcgagcaat acaacgcggt catggccaag tggtccgatc cagaggccga
     4021 ttacgaagcg gtgggggccg agcaatccgc gttggaagac aagatcaacg cggttggggc
     4081 gtgggacatc gagcgcaacg tggagatcgc gatggatgcg ttgcgctgtc cgcctgacga
     4141 cgcgtccgtc agcacactct ccggtggtga gaagcgcagg gtggctctgt gtcggctgct
     4201 ccttcagcac cccgacctgc tgctgctcga cgagccgacc aaccacctcg atgccgagtc
     4261 ggtcgagtgg ctcgagcggt tcctggcgga gtattcgggg accgtcgtcg ccgtcacgca
     4321 cgatcgctac ttcctcgaca acgtcgcgca gtggatcctg gagctggacc gggggagggg
     4381 catccccttc gcgggcaact attccagctg gctcgaacag aagctgggtc ggctcgcggg
     4441 cgagcagaag caggtggacg cccggcaacg cacgctcgcg cgtgagctcg attgggtgcg
     4501 catgggcgcg aaggcccgtc aagccaaggg aaaggcccga ctcaccgcct acgagaagct
     4561 gcttgatgag gcaaacgacg cccgcggagt ccagcagcgc gagctcgaga tcgccatccc
     4621 gcccgggcct cgtctgggcg agacggtcat cgacgtcaag ggcctgcgca agggctacgg
     4681 cgacaggtta ctgatcgagg acctgtcgtt ctccctgccg cgcgccggca tcgtggggat
     4741 catcgggccc aacggcgccg gcaagacga
//
LOCUS       DATLCO010000077         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_88668, whole genome shotgun sequence.
ACCESSION   DATLCO010000077 DATLCO010000000
VERSION     DATLCO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88668"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1977
                     /locus_tag="VMZ33_01980"
     CDS             343..1977
                     /locus_tag="VMZ33_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HUR16030.1"
                     /translation="MAVLTTRVDSSSNEARANATQMRALVGELREKTRQVSERGAAGD
                     ERSIARHRERGKLPVRERIDRLVDPGSPFLELSPLAANGMYDDEAPGAGIVTGIGQVA
                     GTDVVIVANDATVKGGTYYPMTVKKHLRAQEIAQQNRLPCIYLVDSGGAFLPLQGDVF
                     PDREHFGRIFYNQAQMSARGIPQIALVMGSSTAGGAYVPAMSDETVIVRGTGTIFLGG
                     PPLVKAATGEDVTAEDLGGASVHARISGVADHEALDDEHALAIGREIVQSLSWRKADA
                     PWDRLPSRPPAVATPDPDDEAGTADDPYGMYGAFSPDLRKGYDVHEVIARLVDGSEFH
                     EFKAGYGETLVCGFAHLDGYPVGILANNGVLFSQSALKGAHFIELACQRRIPLVFLQN
                     ITGFMVGREYEAGGIAKDGAKLVTAVATAEVPKFTVIIGGSFGAGNYGMAGRAYGSRG
                     LWMWPNARISVMGGEQAAIVLSMVGTFTNEKERDAYRDAIRAKYELEGNPYYSTARLW
                     DDGVIDPAQTRDVLALAIGASMNAPIQETKFGVFRM"
     gene            1974..>2528
                     /locus_tag="VMZ33_01985"
     CDS             1974..>2528
                     /locus_tag="VMZ33_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16031.1"
                     /translation="MSSAAIGARWPFACFVALGLFWGAWAALIPDMKAQVDATDGELG
                     VALLFMGVGAIPAMLVTGRLWRRFGWWLLPVGGLLFAASMLPPIFATTPLMLAGTALL
                     FGAGSGFLDVSMNAAVSDVEAARGSRLMFAAHALFSLGVLIAAMATGLAREMGAQPGH
                     VLTVVAAVTAAIAVGTIRSVRVARS"
BASE COUNT          404 a          838 c          870 g          416 t
ORIGIN      
        1 ctcggggact cgcgagctcg agggatcctt ctcgacacgc ccgatcgccg cgtcatcgtg
       61 atcatcgtcg gctcgcagca ttcggccggt cacgaggcat tcctcgccga ggcgatgccg
      121 gttgtcgaga gcttccagtt cggccagtag gcgcggcaat tcgaacgggc atgagccacg
      181 ttcgtgtcac tggtaacccc ctggtgtcac tgtccgtgac ataaccgcca cgagcggccg
      241 tctcgagggg ctcggcggcc acgctcgggg cccggacgca ctcgctgccg gggagtcagc
      301 gctgggacgc tgggtggcac cgtggaccct atgatcggtc gcgtggcggt cctgacgacg
      361 cgtgtcgatt caagcagcaa cgaggcacgc gctaacgcga cgcagatgcg cgccctcgtt
      421 ggggagctaa gagagaagac gcgacaggtc agcgagcgcg gcgcggctgg ggatgagcgt
      481 tccatcgcgc ggcatcgcga gcgcggcaag ctccccgtcc gcgaacgcat cgacaggctg
      541 gtcgacccag gctcgccctt tcttgagctg agccccttgg ccgcgaacgg catgtacgac
      601 gatgaggcac ccggcgcggg aatcgtcacg ggcataggcc aagtcgctgg cacggacgtc
      661 gtgatcgtcg ccaacgacgc cactgtcaag ggcggcacct actaccccat gaccgtcaag
      721 aaacacctcc gcgcccagga gatcgcgcag cagaaccggc tgccgtgcat ctacctggtg
      781 gacagcggcg gcgcctttct gccgctgcag ggagacgtct tccccgatcg cgagcacttc
      841 ggccgcatct tctacaacca ggcccagatg tcggccaggg gcatcccgca gatcgcgctg
      901 gtgatgggat ccagcaccgc gggcggcgcg tacgtgccag cgatgagcga cgagacggtc
      961 atcgtccgcg gcacgggcac gatcttcctc ggcggcccgc cactcgtgaa ggcggcgacc
     1021 ggggaggacg tcacggccga agatctcggc ggagccagcg tccacgcccg catctctggt
     1081 gtcgccgacc acgaggcgct cgatgacgag catgcgttgg cgatcggacg ggagatcgtc
     1141 caaagcctga gctggcgcaa ggccgacgca ccttgggacc gcctcccgag tcggccccct
     1201 gcggtcgcaa cccctgaccc agacgacgaa gcgggcacgg ccgacgatcc atacggcatg
     1261 tacggcgcgt tcagcccgga cctccgaaag ggttacgacg tccacgaggt catcgctcga
     1321 ctggtcgacg gctccgagtt ccatgagttc aaggctggct acggtgagac gctcgtctgt
     1381 ggcttcgctc atctcgacgg ctatccggtc ggcatccttg ccaacaacgg cgtcctgttc
     1441 agtcagtcgg cgctgaaggg ggcgcacttc atcgagctgg catgccagcg ccgcatcccg
     1501 ctggtgttcc tgcagaacat caccgggttc atggtcggcc gcgagtacga ggccggcggc
     1561 atcgccaagg acggcgcgaa gctcgtcacg gccgtggcca ccgccgaggt gcccaagttc
     1621 acggtcatca tcggcggctc gttcggcgcc gggaactacg gcatggccgg tcgcgcgtac
     1681 ggctcgcgcg gcctgtggat gtggcccaac gcccgcatca gcgtcatggg cggcgagcag
     1741 gcagcgatcg tgctgtcgat ggtgggcacg ttcacgaacg aaaaggagcg ggacgcctac
     1801 cgcgacgcga tccgtgccaa gtacgaactc gagggcaatc cctattactc gaccgcacgc
     1861 ctgtgggacg acggcgtgat cgacccagca cagacacgtg acgtgctggc gctggcaatc
     1921 ggcgcatcta tgaacgcgcc aattcaggag accaagttcg gcgtcttccg aatgtgagca
     1981 gcgcggcaat cggcgcgcgc tggccgttcg cgtgtttcgt cgccctcggt ctcttctggg
     2041 gtgcctgggc cgcgctgatc cccgacatga aggcgcaggt cgacgccacg gacggcgagc
     2101 tgggcgtcgc actgctgttc atgggtgtgg gcgcgatccc ggccatgctc gtgacgggtc
     2161 gcctgtggcg gcggttcggc tggtggctcc tccccgtcgg tgggttgctc ttcgcggcgt
     2221 cgatgctgcc gccgatcttc gccacaacac cgctcatgct ggccgggacg gcccttttgt
     2281 tcggcgccgg ctcagggttc ctcgacgtgt cgatgaacgc ggctgtgagc gatgtcgagg
     2341 ctgcccgcgg atcgcggctg atgttcgcgg cgcacgcgct gttctcgctc ggcgtcctga
     2401 tcgcggccat ggcgacgggc ctcgcccgcg agatgggcgc gcagccgggt cacgtcttga
     2461 cggttgtcgc ggcggtgacg gcagcgatcg cagtcggcac gatcagaagc gtccgggttg
     2521 cccgcagc
//
LOCUS       DATLCO010000078         4155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9733571, whole genome shotgun sequence.
ACCESSION   DATLCO010000078 DATLCO010000000
VERSION     DATLCO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9733571"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VMZ33_01990"
     CDS             complement(<1..192)
                     /locus_tag="VMZ33_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HUR16032.1"
                     /translation="MATTLRQRRGADAEQLAVDYLLGLGWRVVGRNVKVGRDEVDVLA
                     LDTGPPRTLVNVEVRSLSVS"
     gene            complement(238..969)
                     /locus_tag="VMZ33_01995"
     CDS             complement(238..969)
                     /locus_tag="VMZ33_01995"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HUR16033.1"
                     /translation="MGVMNPTRRLENRLFRENGHRVILGLDEVGVAPSCGPVVACALA
                     MPLDSRFITGVRDSKMLSAAQREILARRILRRASHVGIGAASVREIDALNIYYATILA
                     MKRAVMRAGDHDFVIVDGRHIRGFEEHAGPYKAVIDGDARIYTISCASIVAKVTRDRL
                     MRKLSLRYPGYGWEHNAGYATPDHRAGMAALGVTPHHRRSFITTQRVLAGEQTALPLF
                     DEPAADLAEVVADLTPSFDVVPIPA"
     gene            complement(1051..1398)
                     /gene="rplS"
                     /locus_tag="VMZ33_02000"
     CDS             complement(1051..1398)
                     /gene="rplS"
                     /locus_tag="VMZ33_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HUR16034.1"
                     /translation="MSVIDEITADQIRTDLPELASGDTVKVSARVKEGNRERIQIFEG
                     TVMRLRGGGINRSITVRALRSGVGVERTFKVNSPRIEKIEVVRHGVARRAQLYFLRQR
                     VGKAASLRERRQS"
     gene            complement(1485..1892)
                     /locus_tag="VMZ33_02005"
     CDS             complement(1485..1892)
                     /locus_tag="VMZ33_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR16035.1"
                     /translation="MSGPTPYLQLPGTAREALTFYGDVFGCNARLFTFEQFGRSDGPP
                     DAIAHGELVDGPVALFASDIAGAQLAFQAQGLMLALLGTAEPSVLRGWFNKLAEGGNI
                     LDELQRRPWGASDGQVIDRFGVQWLIGFEGDEG"
     gene            complement(1889..2575)
                     /gene="trmD"
                     /locus_tag="VMZ33_02010"
     CDS             complement(1889..2575)
                     /gene="trmD"
                     /locus_tag="VMZ33_02010"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HUR16036.1"
                     /translation="MSALRIDVVTIFPEVFPGPLGMSIPGRALERGLATLVAHDLRQW
                     GLGRHRSVDDYPYGGGAGMVMRPEPIHGALEALSGPDTTRVLLDPGGSTFRQSVAHDL
                     AQRPHLVIVCPRYEGVDHRVRSMVDMELSIGDYVLSGGELPAMIVIDAVLRLLPGAID
                     AESLRHESFAEGLLEYPQYTRPPEFVGLSVPDVLVSGNHGAVDEWRRNEARKRTRTGR
                     PDLVEDETAQ"
     gene            complement(2572..3408)
                     /locus_tag="VMZ33_02015"
     CDS             complement(2572..3408)
                     /locus_tag="VMZ33_02015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR16037.1"
                     /translation="MTHDAGLLRDIDAYLDAVPRSATAPEDLGPFTIFRSLAPWPYYA
                     RPRIGFEGGIEAEDVLTLRAAQRERGTTETIEWVHEIAPSMAESAVAAGLQVAAYPLM
                     SLRPADFAPTAPPDGVEVRLLGDDDESFDAAHAVADVGFRFGGTAVGNEGCDARDAAV
                     GATPDAIAEYRRVRAHEGWTISAAAFAVEGPIAVGSHQPIGRATEIVGVATLPTWRRK
                     GLGAAVTTMLVADALVRGVEMIFLSAGSEDVARVYARLGFRRVGTAGAAEPPPRPPET
                     AA"
     gene            complement(3405..3956)
                     /gene="rimM"
                     /locus_tag="VMZ33_02020"
     CDS             complement(3405..3956)
                     /gene="rimM"
                     /locus_tag="VMZ33_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HUR16038.1"
                     /translation="MPSPSTTPRGDEERLAIGLVRGLHGLRGLVRVEILTDNPARFEP
                     GSTLFREGDSRPLTVVSSQRDGPGLLVRFKEISDRNAAESIRDAYLEAAADDSPTDGG
                     FYWHDIVGCAVRTSAGEDLGTVAEVFRVGEGEVYVVRGPRGEILVPAVASIVTELVPA
                     EKRMVVDADALGLNDIDTAAADE"
     gene            complement(3961..>4155)
                     /locus_tag="VMZ33_02025"
     CDS             complement(3961..>4155)
                     /locus_tag="VMZ33_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HUR16039.1"
                     /translation="LVDEPDEVSVKVVDDSEGRVIELHVAESDMGKVIGRNGSVAKAL
                     RTLLKVVAAREGESISLEIV"
BASE COUNT          720 a         1400 c         1347 g          688 t
ORIGIN      
        1 ggacacgctc agactccgga cttccacgtt cacgagagtg cgcggcggcc cggtgtcgag
       61 ggccaggacg tcgacctcgt cgcggccgac cttcacgttg cgtcccacca cccgccagcc
      121 caggcccagc agataatcga cagcaagttg ctcggcgtcg gctccacgcc gctgtcgaag
      181 cgttgttgcc acctcgggac gttaggcggc gtcgcttatc gggcgctcaa cggcgcgtta
      241 cgccggaatt ggcacgacgt cgaacgaggg cgtcagatcg gcgacgacct cagcgaggtc
      301 ggcggccggc tcatcgaaca gcggcagcgc tgtctgctcg ccggcaagca cgcgttgcgt
      361 ggttatgaac gagcgccggt ggtgcggagt cacaccgagc gccgccatcc ccgcgcggtg
      421 atcgggcgtg gcgtatccgg cgttgtgctc ccagccgtag ccgggataac gcagcgacag
      481 cttgcgcatc agtcggtcgc gcgtgacctt ggccacgatc gaagcgcacg agatcgtgta
      541 gatccgcgcg tcgccatcga tgactgcctt atagggtccg gcgtgctcct cgaacccacg
      601 tatgtgccgc ccgtcgacga tcacgaagtc gtgatcaccc gcgcgcatca cggcgcgctt
      661 catcgccagg atggtggcgt agtagatgtt gagggcgtcg atctcacgaa ccgacgccgc
      721 gccgatccca acgtggctcg cgcgtcggag gattcgcctg gccagtattt cgcgttgcgc
      781 ggcggacagc atcttggagt cgcggacccc ggtgatgaaa cggctgtcga gcggcatagc
      841 cagcgcgcac gccacgacag gtccgcagga cggcgcgact ccgacctcgt cgaggccgag
      901 gatcacccgg tgcccgttct cgcggaagag ccggttctcg aggcgtctgg tcggattcat
      961 gacccccatc ggggcgaagc ctagaacacc gcggcaacaa agaagccgcg gatctgtccg
     1021 cggcttcagc ctatctcggg gtatgagcgg tcagctctgc cggcgctccc ggagggacgc
     1081 ggccttgccc acgcgctggc gcaggaagta gagctgtgct cgtcgagcga caccgtggcg
     1141 tacgacttcg atcttctcga tgcgcggcga gttcaccttg aaggtgcgct cgacgccaac
     1201 gccgctgcgc agagcacgaa cggtgatcga tctattgatg ccaccgccgc gcaggcgcat
     1261 gacggtgccc tcgaagatct ggatgcgctc gcggttgcct tccttgactc gcgccgacac
     1321 cttgacggtg tctcctgagg cgagctcagg caggtcggtg cggatctggt ccgcggtgat
     1381 ctcgtcgatg acgctcaagt gtgactctct cgttcagtgc cgggcgcgcg cggacatacc
     1441 gcagcaattc atgtcgggtc gcggagtata catccgccag gcattcaacc ctcgtcaccc
     1501 tcgaagccga tcagccactg aacaccgaag cgatcgatga cctgtccgtc tgacgccccc
     1561 cacggccgac gttgcagttc gtcaaggatg ttcccgccct cggcgagctt gttgaaccag
     1621 ccccgcagga ccgacggctc ggcggtgcca agcagtgcga gcatgagtcc ttgggcttga
     1681 aacgcgagct gtgctccagc aatgtcggac gcgaacagag ccactggacc gtcgaccagc
     1741 tcaccgtgag cgatcgcatc gggcggacca tcggatctcc cgaactgttc gaacgtgaag
     1801 agtcgagcat tgcacccgaa gacatcgccg tagaacgtca gcgcttctcg tgctgtgccg
     1861 ggcagctgta ggtagggcgt cggtccactc attgcgcagt ttcgtcctca accaggtccg
     1921 gccgcccggt acgcgtcctt ttgcgcgcct cattccggcg ccactcatct accgcgccgt
     1981 ggttgccact caccaataca tcgggcacgg acaagcccac gaactccggt gggcgggtgt
     2041 actgcgggta ctcgagcaag ccctcggcga acgactcgtg gcggagcgac tcggcatcga
     2101 tcgcgccggg cagcagacgt agcacggcat cgatgacgat catcgccggc agctcaccgc
     2161 ctgacaggac atagtcgcca atggagagct ccatatcgac catcgatcgg acgcggtggt
     2221 cgacaccctc atagcgcgga caaacgatca cgagatgcgg ccgctgcgcc aggtcgtggg
     2281 cgaccgactg acggaaggta gaaccgcccg gatcgagcag gacgcgcgtc gtgtcggggc
     2341 ctgagagtgc ctccagggcg ccgtggatcg gctcgggccg catgaccatg ccggcgcccc
     2401 cgccgtacgg gtaatcgtcg acgctgcggt ggcgcccaag gccccattgg cgcaaatcgt
     2461 gggcaaccag cgtcgccaga cctcgctcca gcgcccggcc aggaatgctc attcccaatg
     2521 gtccagggaa cacttccgga aagatggtca cgacgtcgat gcggagcgcg ctcatgccgc
     2581 tgtctctggt gggcgcggtg gtggctcggc cgcgccggcg gtgccgaccc tgcgaaatcc
     2641 gagcctggcg tagacccggg cgacgtcctc actgccggcc gacaggaaga tcatctctac
     2701 gccgcgcaca agagcgtcgg ctacgagcat cgtggtgact gcggcaccga ggcccttacg
     2761 tcgccacgtg ggaagagtgg cgacgccgac gatctccgtt gccctgccaa tcggctgatg
     2821 gctcccaact gcaatcggcc cttcaacggc gaaggcggcg gcggagatag tccaaccttc
     2881 gtgggcgcgc acgcgacgat attccgcgat ggcgtctggt gtcgcgccca cggccgcgtc
     2941 gcgagcgtcg cagccctcgt tgccgactgc cgtaccaccg aaccggaagc ccacgtccgc
     3001 gacagcatgg gcagcgtcaa acgactcgtc atcgtccccc agcaagcgaa cttcaactcc
     3061 gtccggcggc gccgtcggtg cgaagtccgc gggcctcagc gacatgagcg ggtaggcggc
     3121 cacctgcaga ccggctgcca cagcggactc ggccatggat ggcgcgatct catgtaccca
     3181 ctcgatggtt tcggtcgtgc ctcgctcgcg ctgcgcggct cggagtgtga gcacatcctc
     3241 tgcctcgatt ccgccttcga agccaatccg tggccgggcg tagtacggcc acggcgccag
     3301 gcttcggaag atggtgaacg ggcctagatc ctcgggcgcg gtggccgatc ggggaacggc
     3361 atcgaggtag gcgtcaatgt ctctcagcag ccctgcgtcg tgggtcactc gtccgcggcc
     3421 gccgtatcga tgtcgttcag gcccagagcg tccgcgtcca cgaccatccg cttttcggcg
     3481 ggtacgagct ccgtgacgat tgacgcaacg gcaggaacca ggatctcgcc gcgtgggccg
     3541 cggacgacat agacctcgcc ctcgccgaca cgaaacacct ctgcgaccgt gcccaggtct
     3601 tcgccggccg acgtccgcac tgcacagcca acgatgtcat gccagtagaa gcccccgtcc
     3661 gtgggcgagt cgtcggcggc cgcctcgaga taggcgtcgc gaatggattc cgccgcgttc
     3721 cggtcggaaa tctccttgaa tcgaacgagc aggccgggtc cgtcgcgttg cgatgaaacc
     3781 acggtcaggg gacgcgagtc gccctcgcga aacagcgtcg agcccggttc gaaccgtgcc
     3841 gggttgtcgg tcaggatttc aacgcggacg agaccccgca gcccatgcag gccacgaacg
     3901 agcccaatgg ccagccgctc ctcgtcgcct cgcggcgtgg tcgagggcga tggcacgggg
     3961 ttagacgatc tcgagtgaga tcgactctcc ctcacgagcg gccacgacct tcagcagggt
     4021 gcgcagggcc ttggcaacgc tgccgttgcg cccgatgacc ttgcccatat cgctctctgc
     4081 gacgtggagc tcaatcacgc ggccttcgga gtcgtcgacc accttgaccg acacctcatc
     4141 cggctcgtcg acgag
//
LOCUS       DATLCO010000079         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3815354, whole genome shotgun sequence.
ACCESSION   DATLCO010000079 DATLCO010000000
VERSION     DATLCO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3815354"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VMZ33_02030"
     CDS             complement(<1..225)
                     /locus_tag="VMZ33_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUR16040.1"
                     /translation="MPVLIGTSGWQYRHWRETFYPRGVKQGEWLEFYAARFDTVESNA
                     AFYRLPEAKTFEAWAQRTPPEFVMAVKASRY"
     gene            complement(241..627)
                     /locus_tag="VMZ33_02035"
     CDS             complement(241..627)
                     /locus_tag="VMZ33_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HUR16041.1"
                     /translation="MAVLRVFLASGLVILASACGGGSGAPTQAPASQPLAAAIACTGS
                     SGLPVAIADFTYTPSTITAAVGGTVTWANGDTAPHSVTFDSGPDCLTISSGGLITATF
                     NAAGSYPYHCSVHPSTPSMKGTVVVQ"
     gene            complement(692..1210)
                     /locus_tag="VMZ33_02040"
     CDS             complement(692..1210)
                     /locus_tag="VMZ33_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HUR16042.1"
                     /translation="MELESGVNGLHVVRKFSDEAVADHDLDAIVDAGRHAGSSKNLQR
                     WHFIVVRSRATLARLADVGPFAGHLAGGAVAVALITPDPAADEPLSVMFDLGRAAQNM
                     VLVAWGRGIGSCPATVYDQELARDILGYPPEMHCEYVLNFGHAAAAETLTRPLRKGGR
                     VALGSLVHHERW"
     gene            1251..1757
                     /locus_tag="VMZ33_02045"
     CDS             1251..1757
                     /locus_tag="VMZ33_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HUR16043.1"
                     /translation="MADQPSGDFPDVIEVVVEIPRGSRNKYEFDSAAGVFRLDRVLSS
                     AVFYNFDYGYIAGTLSEDGDATDALLLIDEPTFPGCHVWARPVGCLEMSDEKGRDFKI
                     LCVAAGDAHQAHVSRLSDIRPHRLVEIEHFFQTYKLLEDKTVEVIGWQDLDRAQAELR
                     ADRSRLES"
     gene            1760..>2113
                     /locus_tag="VMZ33_02050"
     CDS             1760..>2113
                     /locus_tag="VMZ33_02050"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16044.1"
                     /translation="MARKPELWDIAGRQVSISNPDKVYFPETGYTKGDLVRYYIRVAE
                     GALRGAGGRPMALKRFVDGAAGEPFFQKRAPENRPDWIETVELSFPSGRTAHEIVLRD
                     AAQLAWIVNLGCIDLN"
BASE COUNT          373 a          684 c          697 g          359 t
ORIGIN      
        1 gtatcggctg gccttcacgg ccatcacgaa ctcgggcggc gtccgttgcg cccaggcctc
       61 gaatgtcttg gcctcaggca gtcggtagaa ggcggcgttc gactcgacgg tgtcgaaccg
      121 cgcggcgtag aactccagcc actcgccctg ctttacgccc cgcggataga acgtctcacg
      181 ccaatggcgg tattgccagc cggaggtccc gatgaggacc ggcatgcgtc gacttgccga
      241 ctactggacg acgacggtgc ccttcatgct tggagtcgag ggatggacag agcagtgata
      301 cgggtacgag cctgcagcgt tgaacgtggc ggtgatcagc ccgccgctgc tgatagtcag
      361 gcagtcgggg ccactatcaa acgtgacgga gtgcggggcg gtgtcgccgt tggcccaagt
      421 gaccgtgcca cccaccgcag cagtgatggt gctcggcgtg tacgtgaagt cggcaatggc
      481 gactgggagg cctgaactcc ctgtgcaggc gatcgcagcg gccagtggct ggctggctgg
      541 tgcctgagtg ggcgctccgc taccgccgcc acaagccgag gccaggatga ccagcccact
      601 ggcaaggaac acacgaagca cggccatctc ctctggttcg cggctgtagt cggtggattg
      661 tgggcgccgg ctgtcaacgc cgcaagcgcg actaccaccg ttcgtgatgc acgagcgaac
      721 caagcgccac gcgcccaccc ttgcgcaacg gccgggtaag ggtttctgcg gcggccgcgt
      781 gcccgaagtt gagcacgtac tcacagtgca tctcgggcgg gtagcccagg atgtcgcgcg
      841 cgagttcctg atcgtaaacg gtggccggac acgaaccgat gccacgtccc caggcgacga
      901 gcaccatgtt ctgcgccgcg cggcccaggt cgaacatcac tgacagtggc tcgtcagcgg
      961 ctgggtcggg cgtgataagt gctaccgcca ccgctccgcc agccaggtgt ccggcaaatg
     1021 gcccgacgtc ggccagccgc gccagcgtcg cgcgcgaacg gacgacgatg aagtgccagc
     1081 gctgcaggtt cttgctcgag cccgcgtgcc gccccgcgtc aacgatcgcg tccaggtcgt
     1141 ggtccgcgac cgcctcgtcg gaaaacttcc ggacgacatg caggccattc acgccggatt
     1201 cgagttccac cggccggagt ctagactcgg cgccatgcag ggcgcgcgac gtggctgacc
     1261 aacccagcgg ggattttcca gacgtcatcg aggtcgtcgt cgaaatcccg cgcggcagcc
     1321 ggaacaagta cgagttcgac agcgcggcgg gcgttttccg cctcgatcgc gtcctgagct
     1381 ccgccgtctt ctataacttc gattacggct acatcgctgg aacgctctct gaagacggcg
     1441 acgcaaccga cgcactgctg ctgatcgacg agcccacctt tccgggctgc cacgtctggg
     1501 cgcggccagt cggctgcctg gagatgagcg acgagaaagg tcgtgacttc aagatcctgt
     1561 gcgtcgctgc cggtgatgcg catcaggccc atgtttctcg gctgtccgac atcaggccgc
     1621 accggctcgt ggagatcgag cacttcttcc agacgtacaa gctgctcgag gacaaaacgg
     1681 tcgaagtcat cggctggcaa gacctggatc gcgcccaggc tgagctccga gcagatcgca
     1741 gccgtctcga gagttaggcg tggcgcgcaa gcccgagctc tgggacatcg cgggcagaca
     1801 ggtgtcgatc agcaatcccg acaaggtcta cttccccgag acggggtaca ccaagggcga
     1861 tctcgtccgc tattacatcc gtgtggccga gggcgctctg cgcggcgccg gtggccgacc
     1921 gatggctctc aagcgcttcg tggacggtgc cgccggcgag ccgttcttcc agaagcgcgc
     1981 ccctgaaaac cggcccgact ggatcgagac cgtggagctg tcgttcccgt ccggtcggac
     2041 ggcgcatgag attgtgctgc gcgacgcggc tcagctggcc tggatcgtca atctgggttg
     2101 catcgacctc aac
//
LOCUS       DATLCO010000080         5929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9735480, whole genome shotgun sequence.
ACCESSION   DATLCO010000080 DATLCO010000000
VERSION     DATLCO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9735480"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VMZ33_02055"
     CDS             <1..673
                     /locus_tag="VMZ33_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HUR16045.1"
                     /translation="SNGAFSCPMTLRAPYGGGVHGALYHSQSVEAFFAHIPGLKIVIP
                     STPYDTRGLLRASIRDEDPVLFFEHKKMYRSVRGDVPDEDYTIPIGQAKVTREGSDVS
                     IIGYGLMAHYALEAADLLDGEGVSAEVVDLRTLRPLDGETILSSVRKTGKVVVVYEDN
                     RFGGFGAEISAIIAEEAFDHLDGPIVRVTGPDVPGVPYNHVLEDWFMPNPDRIANAVR
                     RLAAY"
     gene            732..2402
                     /locus_tag="VMZ33_02060"
     CDS             732..2402
                     /locus_tag="VMZ33_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16046.1"
                     /translation="MTTASGRGRRVRSPALAALAAAAVLVAAVSAPVLAASSGPSQPA
                     NAGVLEGIPPPSSLSDFNHDGFDDLAIGAPFESVRPTGSPSSLAEAGAVHVLYGSANG
                     LRAEGSQVWNADNAYEVPGAHAGDHFGWSVAVGNFDGDGYGDLAIGAPHTDSSPYSDV
                     GAVFILFGSSAGLTVSGSVRETGSEWFSSVSPAGVQYGFAIAAADSYVAANATLDNDG
                     RTELIVTSPGVNSNTGSIVMRSLADDSRELSHGQGFAVTGASAGDRCGESVAVGDFDN
                     NDGDDVAIGCPMRDYVHPDSGCVAMLYNDFAGRQLICQTTQDVDPPEQNDHFGLALAA
                     GDFDRDNRDDLVVGVPDENLGSLVDAGMVHMFFGASTGGLAADPLSLTQESTGVPGAS
                     AAKDKFGAALAVGDFDGPDGTTGDFTASLAIGLPGDDVGSIDRAGAAVVLYWLAGGMA
                     TSGNQRWTQDSTNVLEKAAGSDRFGSSLAAGRFKGSTTASGLAIGVPLEDLGGPVADA
                     GALNVLYGSPTRLRAAGNQLWHQNSTGIGDVAEGSDRFGSALAAGPLP"
     gene            2501..4018
                     /locus_tag="VMZ33_02065"
     CDS             2501..4018
                     /locus_tag="VMZ33_02065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HUR16047.1"
                     /translation="MPKVQMPQLGESVAEGTIGKWLKKPGDHVDKYEPIVEVITDKVN
                     AEVPSPYEGTLTEILVQEGETVPNNTEIAVIEGANEAAEATGAGASAAAAAAPEAPGA
                     LSTAEAMADETAPQAGTGPAGSNESPGRSVEQPTAEPTQDTQAAPPAAPAAQTPMPMT
                     SAATAVAAPAISGGNGAAGPNGYKGPTTPAVRRLAREHGIELSMIAGSGHSGRVTRED
                     VLKFVESGGAQATQAAAPAAPTQPAATAPAQQGSSTSAPALASAAQASSPVAQGDSLK
                     QPSPMRKAIAAQMSKALAVPVAYTVIEIDMSGVVGLRDRTKGRYQAQEGISLTFDAFV
                     AKATVEALKKHADFNAHYTDDGHWLRKSVNLGVAVAVEDGLVVPVIHNADTLSVHGLN
                     KAIRDLAERSRGQKLRMEDIQGGTFTLDNTGWTGSIITQPIINAPEVGIMTMESIVKR
                     PVVISGPGGDAIGIRPMMYVTLGFDHRATDGAQAGRFVADVKSWLEAVDPNTAIW"
     gene            4036..5034
                     /locus_tag="VMZ33_02070"
     CDS             4036..5034
                     /locus_tag="VMZ33_02070"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUR16048.1"
                     /translation="MRVVITGGAGFIGRAIVERLARRGDDVVALVRDTGRARYLKHDE
                     QVELVTSDLSSIAQLTAQMKKADAVIHAAGMYRIGIQRSERDAMWDANVGATGRVLDA
                     AVAAGAKRIVYVSTANVFGNTRGESPNETFRRDEKLGFLSYYDETKYRAHQAALERIA
                     AGGPIVVVMPSQVYGPHDHSDASAQIDLAYRGRLRYVAFPDTGLAWVHVDDLADGIVA
                     ALDRGRIGESYVMSGDPRRMQQVVEIAARLAGRKPPRLSMPVRVIRGVAPVNDRLGGL
                     PGFPGNLREVIRSAQDVTYWANHDKATTELDFHPRSLEQGIADTWGPGAAKKRSRA"
     gene            5045..>5929
                     /gene="lipA"
                     /locus_tag="VMZ33_02075"
     CDS             5045..>5929
                     /gene="lipA"
                     /locus_tag="VMZ33_02075"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HUR16049.1"
                     /translation="MGRELPMFQAPSAGGAAHGAHTPPTIAMGGGMFNEAAVDGSTAL
                     RRHPEWIKARLPSGENYQDLTRILRGLTLNTVCEEARCPNIGECWEQRTATIMILGDV
                     CTRACGFCAVKTGRPTWTDEDEPRRVAEAIAEMNLEHVVVTSVARDDLPDGGAHIFAE
                     TIRALRQRSPNMGVEVLIPDFNGSDEPLRTVMDAGPDILNHNIETVERLQKPVRKRAR
                     YQRSLDVLARAKEFARDTTGRDGTVHTKSSLMVGLGETRGELSQAFRDLRAVDCDILT
                     VGQYLRPSEQHLPVERYLH"
BASE COUNT         1034 a         1996 c         1950 g          949 t
ORIGIN      
        1 ctcgaacggc gccttctcgt gcccgatgac gctgcgggcg ccatacggcg gcggcgtcca
       61 cggggcgctc tatcactcgc agtcggtcga ggcgttcttc gcgcacatcc cggggctgaa
      121 gatcgtcatc ccctcgacgc catacgacac gcggggattg ctgcgagcgt ccatccgcga
      181 cgaggatccg gtgctgttct tcgagcacaa gaagatgtat cgctccgttc ggggtgacgt
      241 gcccgacgag gactacacga tccccatcgg ccaggcgaag gtgacgcgcg aggggtcaga
      301 cgtctcgatc atcggctacg gcctgatggc gcactacgcg ctcgaggcgg cagacctgct
      361 tgatggcgag ggtgtctccg cggaggtcgt cgacctgcga accctgcgcc cactcgacgg
      421 cgagaccatc ctgtcgtccg tgcgcaagac aggcaaggtc gtcgtcgtct acgaggacaa
      481 ccgcttcggc ggcttcggcg cggagatcag cgcgatcatc gccgaggaag cgttcgacca
      541 cctcgatggg ccgatcgtcc gcgtgactgg ccccgacgtc cccggcgttc cgtacaacca
      601 cgtcctcgag gactggttca tgcccaaccc cgaccggatc gcgaacgcag tgaggcgcct
      661 cgctgcttat tgagcggccc gggggcgtcg acgctcgcac acaatatccg accattagtg
      721 aggcaaaagt agtgaccaca gcatccgggc gcggccggcg tgtgagatcg cctgcgttgg
      781 ccgcgctcgc ggcggcagcg gtgcttgtcg cggccgtgtc ggctcccgtc ctggccgcta
      841 gcagtggtcc atcgcaaccc gcgaacgccg gtgttctaga gggcattcct ccgccgtcat
      901 ccctgtcgga tttcaatcac gatggcttcg atgacctggc tatcggcgcg ccgttcgaat
      961 cggtcaggcc gacgggctcg cccagctcgc tcgctgaggc cggcgcggtc catgttctct
     1021 acggcagcgc gaacggtctg cgagccgagg gttctcaagt ctggaacgcc gacaacgcgt
     1081 acgaggtacc aggcgctcac gcaggcgacc atttcgggtg gtcagtagcg gtggggaact
     1141 tcgacggaga tggttatggc gatcttgcta tcggcgctcc gcacaccgat tcgtctccgt
     1201 acagcgatgt cggtgcggtc ttcattctct tcggcagctc ggctggtctc accgtgtccg
     1261 gctcagtccg cgaaacggga tccgagtggt tcagcagtgt gagtccggcc ggtgttcagt
     1321 acggattcgc gattgccgcc gctgactcgt acgtggcagc aaatgcgact cttgataacg
     1381 acggtcgcac cgaactcatc gtcacgtcgc ctggcgtgaa cagcaacacg ggcagcatcg
     1441 tgatgcggtc gctcgccgac gacagccgcg agctgtccca cggtcagggc ttcgccgtca
     1501 caggcgcgtc ggccggcgac aggtgcggcg agtcggtcgc cgtcggtgac ttcgacaaca
     1561 acgatggtga tgatgtcgcg atcggctgcc ccatgcgtga ttacgtccac cccgactccg
     1621 gatgcgtagc catgctctac aacgatttcg ccggtcgcca gctcatctgc cagacgacgc
     1681 aggatgtcga cccgcccgag cagaacgacc atttcggctt ggccctcgcc gccggagact
     1741 tcgatcgcga caaccgcgat gatctcgtgg tcggcgtgcc ggacgagaac ctcggatcgc
     1801 ttgtcgatgc cggcatggtc cacatgttct tcggcgcctc gacgggcggc ctggcggcag
     1861 acccgctctc gctgacgcag gagtcgaccg gcgtacctgg cgcgtcggcc gccaaggaca
     1921 agttcggcgc cgccctcgcc gtgggcgact ttgatggacc ggatggaacg accggcgatt
     1981 tcacggcgag cctcgcgatc gggttgcccg gcgatgacgt cggcagcatc gatcgggccg
     2041 gggcggcggt cgtgctctat tggctggcag gcggaatggc cacgtccggc aatcagcgct
     2101 ggacgcagga ctcgacgaac gttctcgaaa aggccgcagg tagcgatcga tttggatcat
     2161 ctctggccgc cggccgcttc aagggatcga cgacggcgtc aggtctggcc atcggcgtgc
     2221 cgctggagga cctcggcggg cccgtcgccg acgccggcgc gctgaacgtc ctgtatggct
     2281 cgccgacgag gctccgtgcc gccggcaatc agctctggca ccagaattcg accggcatcg
     2341 gcgacgtagc cgagggctcg gaccgcttcg gcagcgccct cgcggccggt cccttgccct
     2401 agcaccgggt agacctttct ggacgatcgc gcgtgcgatc agcgacactt agcgactgcc
     2461 gcccagacac tcgcgttgac cgtccaggag aagacatcgc atgcccaagg tccagatgcc
     2521 gcaactcggc gagagtgtcg ccgagggcac gatcggaaag tggctgaaga agcccggcga
     2581 ccacgtcgac aaatacgagc cgatcgtcga ggtcatcacc gataaggtga acgccgaggt
     2641 cccgtcgccg tacgagggca ccttgaccga gatcctcgtt caggagggcg agacggtccc
     2701 gaacaacact gagattgcgg tcatcgaggg cgccaacgaa gccgccgagg caaccggtgc
     2761 cggagcgtcc gcagctgcag ccgcggcccc agaagcgccc ggagcacttt ccacggccga
     2821 ggcgatggct gatgagacgg ctccgcaggc cgggaccggc cccgcaggct ccaatgagag
     2881 tcctggtcga tcggtcgaac agccgactgc cgagcccacc caggacaccc aggctgcccc
     2941 gccggccgcg cccgcggcgc agacgccgat gcccatgaca tcggccgcga cagccgttgc
     3001 tgcaccggcc atctcgggtg gcaacggagc cgctggcccg aacggctaca agggcccgac
     3061 cacgcctgcc gtccggcgac ttgcccgcga gcacgggatc gagctttcga tgatcgccgg
     3121 cagcggccac agtggtcgcg tcacacgcga agacgtgctg aagttcgtgg agtccggcgg
     3181 cgcgcaggcg acgcaggccg cagcgccagc cgcgccgacg cagcccgccg ccaccgcacc
     3241 cgctcagcaa ggctcgtcaa cctcggctcc ggccctggcc tccgcagcgc aggcctcttc
     3301 gcccgtcgcc cagggcgact cgctgaagca gccgtcgccg atgcgcaagg ccatcgccgc
     3361 ccagatgtcg aaggcgcttg cggtaccagt ggcctacacg gtcatcgaga tcgacatgtc
     3421 gggcgtcgtc ggtctgcgcg accgcaccaa gggtcggtac caggcacagg agggcatcag
     3481 cctcacgttt gacgcgttcg tggccaaggc caccgttgag gccctcaaga agcacgccga
     3541 cttcaacgcg cactacaccg acgacggcca ctggcttcgt aagtcggtca acctgggagt
     3601 tgccgtagcc gtcgaggacg gactcgtggt gccggtgatc cacaacgccg acacgctcag
     3661 cgtccatggc ctcaacaagg ccattcgtga cctcgccgag cgcagtcgcg ggcagaagct
     3721 gcgcatggag gacatccagg gcggcacgtt tacgcttgac aacaccggtt ggacaggctc
     3781 aatcatcacg cagccgatca tcaacgcccc cgaggtcggg atcatgacca tggagtccat
     3841 cgtcaagcga cccgtcgtga tcagcggacc gggcggggac gccatcggca tccggccaat
     3901 gatgtacgtg acccttggct tcgaccatcg ggcgacggac ggcgcccagg ccggacgctt
     3961 tgtcgcggac gtgaagagct ggctcgaggc ggtcgacccc aacaccgcca tctggtaggc
     4021 aaccctgcag gttccgtgcg cgtcgtcata accggcggtg caggcttcat cggccgcgcg
     4081 atcgtcgaac gcctggccag gcgtggcgat gacgtcgtcg cgctggttcg cgacaccggc
     4141 cgcgcccgat acctcaagca cgatgagcag gtcgagctcg tgacgagtga tttgtctagc
     4201 atcgcgcagc tgacagcgca gatgaagaag gccgatgcgg tcatccatgc tgccggcatg
     4261 taccgaatcg gcatccagcg cagcgagcgc gacgccatgt gggacgccaa cgtgggcgca
     4321 actgggcgcg tcctcgacgc ggcagtcgcg gcaggggcga agcgcatcgt ctacgtgtcc
     4381 acggccaacg tgttcggcaa cacccgcggc gaatcaccga acgagacctt tcgtcgtgac
     4441 gagaaacttg ggttcctgtc gtactacgac gaaaccaagt accgcgccca ccaggcggcc
     4501 cttgagcgaa ttgcagccgg cgggcccatc gttgtcgtca tgcccagcca ggtctacggc
     4561 ccgcacgacc attccgatgc aagtgcgcaa atcgatctgg cctatcgcgg caggctgcgc
     4621 tacgtcgcgt ttccggacac gggcctcgca tgggtgcacg tcgacgacct ggccgatggg
     4681 atcgtcgccg cgctggatcg cggccgcatc ggtgagtcct acgtcatgtc gggtgatccg
     4741 cgccgcatgc agcaggtggt cgaaatagcc gctcgcctcg ccggtagaaa gccgcctcgc
     4801 ttgagcatgc ccgttcgcgt gatccgcggc gtcgcaccgg tcaacgaccg gctcggtggg
     4861 cttcccggtt ttcctggaaa ccttcgggag gtgatccgat ccgcacagga tgtgacctat
     4921 tgggccaacc acgacaaggc gacgaccgag ctggactttc acccgcgctc gcttgagcag
     4981 ggcatcgccg atacctgggg acccggcgcc gccaaaaagc ggtctcgggc gtagagtcgc
     5041 gtcgatgggt cgcgagctac cgatgttcca ggcgccgtcc gcgggtggcg cggcgcacgg
     5101 cgcgcacacg ccaccgacga ttgccatggg cggcggcatg tttaacgagg cggccgtgga
     5161 cggctcgacc gccctgcgcc gccaccccga gtggatcaag gcgcggctcc cgtccgggga
     5221 gaactaccag gacctgacac gcatcctgcg cgggctgacc ctcaacaccg tctgtgagga
     5281 ggctcgttgc cccaacatcg gggagtgctg ggagcagcgc acggcgacga tcatgattct
     5341 cggcgacgtc tgtacccggg cctgcggttt ctgtgcagtg aagaccggcc ggcccacgtg
     5401 gaccgacgag gatgagccgc gtcgcgtcgc tgaggctatc gccgagatga acctcgagca
     5461 cgtggtcgtg acaagtgtgg cgcgcgacga cctccccgac ggcggcgctc acatctttgc
     5521 cgagaccatc cgcgcccttc ggcagcgcag cccgaacatg ggagtggagg ttctgatccc
     5581 cgatttcaac ggcagcgacg agccgctacg cacggtcatg gacgccggac cggacatcct
     5641 gaatcacaac atcgagaccg ttgagcggct ccagaagccg gttcgcaagc gcgctcgtta
     5701 ccagcgctcg ctcgacgtgc tggcacgtgc caaggagttc gcacgcgaca caacgggccg
     5761 cgacggcact gttcacacga agtcgagcct gatggtcggc ttgggcgaga cccggggaga
     5821 gctcagtcag gctttccgcg acctgcgcgc ggtcgactgc gacatcctga ccgtcggcca
     5881 atacctccgt ccgtcggagc agcacctgcc ggtcgagcgc tacctccac
//
LOCUS       DATLCO010000081         4190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9735515, whole genome shotgun sequence.
ACCESSION   DATLCO010000081 DATLCO010000000
VERSION     DATLCO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9735515"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VMZ33_02080"
     CDS             <1..673
                     /locus_tag="VMZ33_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HUR16050.1"
                     /translation="SNGAFSCPMTLRAPYGGGVHGALYHSQSVEAFFAHIPGLKIVIP
                     STPYDTRGLLRASIRDEDPVLFFEHKKMYRSVRGDVPDEDYTIPIGQAKVTREGSDVS
                     IIGYGLMAHYALEAADLLDGEGVSAEVVDLRTLRPLDGETILSSVRKTGKVVVVYEDN
                     RFGGFGAEISAIIAEEAFDHLDGPIVRVTGPDVPGVPYNHVLEDWFMPNPDRIANAVR
                     RLAAY"
     gene            762..2279
                     /locus_tag="VMZ33_02085"
     CDS             762..2279
                     /locus_tag="VMZ33_02085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HUR16051.1"
                     /translation="MPKVQMPQLGESVAEGTIGKWLKKPGDHVDKYEPIVEVITDKVN
                     AEVPSPYEGTLTEILVQEGETVPNNTEIAVIEGANEAAEATGAGASAAAAAAPEAPGV
                     VATAEAMADETAPQAGTGPAGSNESPGRSVEQPTAELTQATQATPPAAPAAQTPMPMT
                     SAATAVAAPAISGGNGAAGPNGYKGPTTPAVRRLAREHGIELSMIAGSGHSGRVTRED
                     VLKFVESGGAQATPSAQGAAPTQPAATAPAQQGSSGSAPAVASAAQASSPVAQGDSLK
                     QPSPMRKAIAAQMSKALAVPVAYTVIEIDMSGVVSLRDRTKGQYQAQEGISLTFDAFV
                     AKATVEALKKHADFNAHYTDEGHWLRKSVNLGVAVAVEDGLVVPVIQNADTLSIHGLN
                     KAIRDLAERSRGQKLRMEDIQGGTFTLDNTGWTGSIITQPIINAPEVGIMTMESIVKR
                     PVVISGPNGDAIGIRPMMYVTLGFDHRATDGAQAGRFVADVKSWLEAVDSNTAIW"
     gene            2297..3295
                     /locus_tag="VMZ33_02090"
     CDS             2297..3295
                     /locus_tag="VMZ33_02090"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR16052.1"
                     /translation="MRVVITGGAGFIGRAIVERLAKRGDDVVALVRDTGRARYLKQDD
                     KVELVTSDLSSIAQLTAQMKKADAVIHAAGMYRIGIQRSERDAMWDANVGAAERVLDA
                     AVAAGAKRIVYVSTANVFGNTHGESPNETFRRDEKLGFLSYYDETKYRAHQAALERIA
                     AGAPIVVVMPSQVYGPHDHSDASAQIDLAYRGKLRYVAFPDTGLAWVHVDDLADGIVA
                     ALDRGRLGESYVMSGDPRRMRQVVEIAARLAGRKPPRFSMPVRVIRGIAPVNDRLGGL
                     PGFPGDLREVIRSAQDVTYWANHDKATAELGFHPRSIEQGILDTWGPGAAKKRSRA"
     gene            3306..>4190
                     /gene="lipA"
                     /locus_tag="VMZ33_02095"
     CDS             3306..>4190
                     /gene="lipA"
                     /locus_tag="VMZ33_02095"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HUR16053.1"
                     /translation="MGRELPMFQEPSAGGAAHGAHMPPTIAMGGGMFNEEATDGSTAL
                     RRHPEWIKARLPSGENYQDLKRILRGLTLNTVCEEARCPNIGECWEQRTATIMILGDV
                     CTRACGFCAVKTGRPTWTDEDEPRRVAEAIAEMNLEHVVVTSVARDDLSDGGAHIFAE
                     TIRALRQRSPNMGVEVLIPDFNGSDEPLRTVMDAGPDILNHNIETVERLQKPVRKRAR
                     YQRSLDVLARAKEFARDTTGRDGSVHTKSSLMVGLGETRGELSQAFRDLRAVDCDILT
                     VGQYLRPSEQHLPVERYLH"
BASE COUNT          765 a         1419 c         1376 g          630 t
ORIGIN      
        1 ctcgaacggc gccttctcgt gcccgatgac gctgcgggcg ccatacggcg gcggcgtcca
       61 cggggcgctc tatcactcgc agtcggtcga ggcgttcttc gcgcacatcc cggggctgaa
      121 gatcgtcatc ccctcgacgc cctacgacac gcggggatta ctgcgagcgt cgatccgcga
      181 cgaggatccg gtgctgttct tcgaacacaa gaagatgtat cgctccgttc ggggtgacgt
      241 gcccgacgag gactacacga tccccatcgg ccaggcgaag gtgacgcgcg aggggtcaga
      301 cgtctcgatc atcggctacg gcctgatggc acactacgcg ctcgaggcgg cagacctgct
      361 tgatggcgag ggcgtctcgg cagaggtcgt cgacctgcgt accctgcgcc cactggacgg
      421 cgagacgatc ctgtcgtccg tgcgcaagac gggcaaggtc gtcgtcgtct acgaggacaa
      481 ccgcttcggc ggcttcggcg cggagatcag cgcgatcatc gccgaggagg cgttcgacca
      541 cctcgatggg ccgatcgtcc gcgtgactgg ccccgacgtc cccggcgtcc cgtacaacca
      601 cgtcctcgag gactggttca tgcccaaccc cgatcgcata gcgaacgccg tgagacgcct
      661 cgccgcttac tgaggcgccc gccaggccag cccgcacgtg tggctcatct gcgagtggcg
      721 actaaactag gcgagccgtc cctgaggaga gaaacaacga catgcccaag gtccagatgc
      781 cgcaactcgg cgagagtgtc gccgagggca cgatcggaaa gtggctgaag aagcccggcg
      841 accacgtcga caaatacgag ccgatcgtcg aggtcatcac cgacaaggtg aacgccgagg
      901 tcccgtcgcc atacgagggc accttgaccg agatcctcgt ccaggagggc gagacggtcc
      961 cgaacaacac tgagattgcg gtcatcgagg gcgccaacga agccgccgag gcaaccggtg
     1021 ccggagcgtc cgcagcggca gccgcggccc cagaggcgcc cggcgtagtc gccacggccg
     1081 aggcgatggc tgatgagacg gccccgcagg ccggcaccgg ccccgcaggc tccaatgaga
     1141 gtccgggtcg atcggtcgaa cagccgactg ccgagcttac ccaggccacc caggctaccc
     1201 cgccggccgc gcccgcggcg cagacgccga tgcccatgac atcggccgcg acagccgtgg
     1261 ctgcgccggc catctcgggt ggcaacggag ccgctggccc gaacggctac aagggaccaa
     1321 ccacgcctgc cgtccggcga cttgcccgcg agcacgggat cgagctttcg atgatcgccg
     1381 gcagcggcca cagcggtcgc gtcacacgcg aggacgtgct gaagttcgtc gagtcgggcg
     1441 gcgcgcaggc gacgccgtcg gcgcaggggg cagctccgac ccagcccgcc gccacggcac
     1501 ccgcacagca aggctcgtca ggctcggctc cggccgtggc ctccgcagcg caggcttctt
     1561 cgcccgtcgc ccagggcgac tcgctgaagc agccgtcgcc gatgcgcaag gccatcgccg
     1621 cccagatgtc gaaggcgctt gcggtaccag tggcctacac cgtcatcgag atcgacatgt
     1681 cgggcgtcgt cagtctgcgc gaccgcacca agggtcagta ccaggcacag gagggcatca
     1741 gcctcacgtt tgacgcgttc gtggccaagg ccaccgttga ggccctcaag aagcacgccg
     1801 acttcaacgc gcactacacc gacgagggcc actggcttcg taagtcggtc aacctgggag
     1861 ttgccgtagc cgtcgaggac ggactcgtgg tgccggtgat ccagaacgcc gacaccctca
     1921 gcatccatgg cctcaacaag gccatccgcg acctcgccga gcgcagtcgc ggccagaagc
     1981 tgcgcatgga ggacatccag ggcggcacgt tcacgcttga caacaccggt tggacaggct
     2041 cgatcatcac gcagccgatc atcaacgcgc ccgaggtcgg gatcatgacc atggagtcga
     2101 tcgtcaagcg acccgtcgtg atcagcggac cgaatgggga cgcgatcggc atccgaccga
     2161 tgatgtacgt gacccttggt ttcgaccatc gggcaacgga cggcgcccag gccggccgct
     2221 tcgtcgctga cgtgaagagc tggctcgagg cggtggactc caacaccgcc atctggtagg
     2281 caaccctgcg ggttccgtgc gcgtcgtcat aaccggcggc gcaggcttca tcggccgcgc
     2341 gatcgtcgaa cgcctggcca agcgtggcga tgacgtcgtc gcgctggtcc gggacactgg
     2401 ccgcgcccgc tacctcaagc aagacgacaa ggtcgagctc gtgacaagcg acctgtcgag
     2461 catcgcgcag ctgactgcgc agatgaagaa ggccgatgca gtgatccatg ctgccggcat
     2521 gtaccgaatc ggcatccagc gcagcgagcg cgacgccatg tgggacgcca acgtgggcgc
     2581 agctgagcgc gtcctcgacg cggcggtcgc ggcaggggcg aagcgcatcg tctacgtgtc
     2641 cacggccaac gtcttcggca acacccacgg cgaatcaccg aacgagacct ttcgtcgtga
     2701 cgagaagctt gggtttttgt cgtactacga cgaaaccaag taccgcgccc accaggcggc
     2761 ccttgagcgt attgcagccg gcgcgcccat cgtcgtcgtc atgcccagcc aggtctacgg
     2821 cccgcacgac cattccgatg caagtgcgca aatcgacctg gcctatcgcg gcaagctgcg
     2881 ctacgtcgcg tttccggaca cgggccttgc atgggtgcac gtcgacgacc tggccgacgg
     2941 gatcgtcgcc gctctggatc gcggccgcct cggtgagtcc tacgtcatgt cgggtgatcc
     3001 gcgccgcatg aggcaggtgg tcgaaatagc cgctcgcctc gccggtagaa agccgccgcg
     3061 cttcagcatg cccgtccgcg tgatccgcgg catcgcaccg gtcaacgacc ggctcggtgg
     3121 gcttcccggt tttcctggag accttcggga ggtgatccgt tccgcacagg atgtgaccta
     3181 ttgggccaac cacgacaagg cgaccgctga gctgggcttc catccgcgct cgattgaaca
     3241 gggcatcctc gatacctggg gacccggcgc cgccaagaag cggtctcggg cgtagagtcg
     3301 cgtcgatggg tcgcgagcta ccgatgttcc aggaaccgtc cgcgggtggc gcggcgcacg
     3361 gcgcgcacat gccaccgacg atcgcgatgg gtggcgggat gttcaacgaa gaggccacgg
     3421 atggctcgac cgccctgcgc cgccaccccg agtggatcaa ggcgcggctt ccgtccggtg
     3481 agaactacca ggacctgaaa cgcatcctgc gcgggctgac gctcaacacc gtttgtgagg
     3541 aggcgcgttg ccccaacatc ggcgagtgct gggagcagcg cacggccacg atcatgattc
     3601 tcggcgacgt ctgtacccgc gcctgcggtt tctgtgcggt gaagaccggc cgtcccacgt
     3661 ggaccgacga ggacgagccg cgtcgcgtcg ctgaggcgat cgccgagatg aacctggagc
     3721 acgtcgtcgt gaccagtgtg gcgcgcgatg acctctccga cggtggcgct cacatctttg
     3781 ctgagaccat ccgcgccctt cggcagcgca gcccgaacat gggagtggag gtcctgatcc
     3841 ccgatttcaa cggcagcgac gagccgctac gcacggtcat ggacgccgga ccggacatcc
     3901 tgaatcacaa tatcgagacc gttgagcggc tccagaagcc ggttcgcaag cgcgctcgtt
     3961 accagcggtc gctcgacgtg ctggcacgtg ccaaggagtt cgcacgcgac acaacgggcc
     4021 gcgacggtag cgttcacacg aagtcgagtc tgatggtcgg cttgggcgag acccggggag
     4081 agctcagtca ggctttccgc gacctgcgcg cggtcgactg cgacatcctg accgtcggcc
     4141 aatacctccg tccgtcggag cagcacctgc cggtcgagcg ctacctccac
//
LOCUS       DATLCO010000082         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2329345, whole genome shotgun sequence.
ACCESSION   DATLCO010000082 DATLCO010000000
VERSION     DATLCO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329345"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VMZ33_02100"
     CDS             <1..387
                     /locus_tag="VMZ33_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16054.1"
                     /translation="VFMPKRRVLNYSLDANQSGDSFLFNLFISPIAIIVNFVASLPGA
                     VLALFRGQINSRVPATLLIAIGAFVIASGDTFARLGSTDFFQLGKLIGVVLLFCGFLV
                     SVDVFSEIRIPFTHRVLRSARRERVC"
     gene            293..1339
                     /locus_tag="VMZ33_02105"
     CDS             293..1339
                     /locus_tag="VMZ33_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUR16055.1"
                     /translation="MASWSRLTSSARSASRSRIACCVRPDASESVDHLNRLVVGSLLV
                     ATAAACFGTLGYVSRNAADLGMGTFPYVAWRGLIATLAMLPLVAFMTQRFPPRTNSRL
                     PPNRRRALLAAALLGSVVNIATFAAFSRTTIAVVLICFYTFPAIVSIAAVRLYGERID
                     RFRGTALIVSGAGLILVLVAPLLGATAVSLDPIGIGLALIAAISQASFILIAGRGFRP
                     WRPWSVGTVLILSAGAVAAILCVVTLDVAGLSRPFAEPQTWIWIAFGALIGGAIPTTI
                     FMVGIGWIGPSRAAVLMTIEPLVGVLLAGALLGETPSPLQLVGGAMVVAAAGALQLVP
                     PRKVAPEPEFGPLL"
     gene            1448..2326
                     /gene="galU"
                     /locus_tag="VMZ33_02110"
     CDS             1448..2326
                     /gene="galU"
                     /locus_tag="VMZ33_02110"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HUR16056.1"
                     /translation="MRVRKAVFPAAGWGTRFLPATKAQPKEMLPLVDKPVIQYGVEEA
                     VAAGVDQVVIVTSSQKRAIEDHFDHNYELEHLLEEKGDIDMLRRVRQIGDMVQISYVR
                     QKEQLGLGHAVLMAKDVVGHEPFAVILSDDVTVADRPVIGQLIEVYRQTHSSVVAVME
                     VSREETSRYGIIDPDPDFATTDPRLVKVRGVVEKPRPEDAPSNLAIIGRYVLTPRIFE
                     KLEETPRGAGGEIQLTDAIMALTSEQDVYAYAFEGRRYDAGTTMGWLKASVELALERD
                     DFGAEFRRHLSTLDLS"
BASE COUNT          407 a          859 c          796 g          485 t
ORIGIN      
        1 gtcttcatgc ccaagcgccg cgtcctgaac tactcgctcg acgccaacca gtcgggcgat
       61 tccttccttt tcaacctgtt catcagcccc atcgcgatca tcgtcaactt cgtcgcctcc
      121 ctgcccggcg cagtcctggc gctcttccgc ggccagatca acagccgcgt gccggcgacg
      181 ctgctgatcg ctatcggcgc gttcgtcatc gccagcggcg acacgttcgc tcgcctcggt
      241 tcaacggact tcttccagct cgggaagctg atcggcgtcg tcctgttgtt ctgtggcttc
      301 ctggtctcgg ttgacgtctt cagcgagatc cgcatcccgt tcacgcatcg cgtgctgcgt
      361 tcggcccgac gcgagcgagt ctgttgacca cctgaaccgg ctggtcgtcg gctcgctgct
      421 ggtcgccact gcggccgcgt gctttggcac gctcggctac gtgagtcgca acgcggctga
      481 cctggggatg ggcacattcc cttacgtcgc ctggcgcggg ctcatcgcca cgttggccat
      541 gctcccgttg gtggcgttca tgacgcaacg gttcccgccg cggaccaact cccgcctccc
      601 gccgaaccga cgccgggcgc tgctggccgc ggcgttgctc ggctccgtgg tgaacatcgc
      661 cacgttcgca gcgttttcgc ggacgacgat cgccgttgtg ctgatctgct tctacacgtt
      721 cccggccatc gtctcgatcg ccgcggtacg cctctacggc gagcgcatcg accgcttccg
      781 cggcacggcg ctcatcgtct cgggagctgg cctcatcctc gtgctcgtcg cgccgttact
      841 cggcgcaacg gcggtaagcc tcgatccaat cgggatcggc ctggccctca tcgctgcaat
      901 aagtcaggcg agcttcatcc tgatcgccgg ccgcggcttt cgaccgtggc ggccgtggag
      961 cgtgggcacg gtgctgatcc tctccgccgg agcggtcgcg gccatcctct gtgtcgtgac
     1021 gctggacgtc gctggactga gtcgtccgtt tgctgagcca cagacgtgga tatggatcgc
     1081 gtttggggcg ctcattggcg gagccattcc aacgacgatc ttcatggtcg gaatcggctg
     1141 gatcgggccc tcgcgggcgg cggttctgat gaccatcgag ccgctggtcg gagtccttct
     1201 cgccggcgcc ctgctgggtg agacgccatc tccacttcag ctcgttggag gcgccatggt
     1261 ggtcgccgcg gcgggcgcct tacagctcgt cccgccgcga aaagtcgcgc cggagcccga
     1321 gttcggtccg ctgttgtaag gctgccgacg aacccccaca tctatactcg acgcgaccag
     1381 ggagcctgtc gacagccgcg ccagaccatg cggccggcca ctccagttcc cgagcacgag
     1441 gcaccttttg cgcgtccgaa aagccgtttt tccagctgca ggctggggca cacgtttcct
     1501 gcctgccacg aaagcacagc ccaaggaaat gctgccgctc gtcgataagc cggtgatcca
     1561 gtacggcgtc gaggaggctg ttgcggcagg cgtcgaccag gtcgtcatcg tcaccagcag
     1621 ccagaagcgc gcgattgagg atcacttcga ccataactac gagctcgagc acctgctcga
     1681 ggaaaaaggc gacatcgaca tgcttcgccg agtccggcag atcggcgaca tggtccagat
     1741 cagctacgtg cgccagaagg agcagctcgg cctcggccat gcagtcctta tggccaagga
     1801 tgtcgtcggc cacgagccgt tcgctgtgat cctctcggac gacgtcacgg tcgcggacag
     1861 gccggtaatc ggccagctca tcgaggtata tcggcagacg cactcatcgg tcgtggccgt
     1921 gatggaggtt tcgcgcgaag agacgagccg ttacggcatc atcgaccccg atccggactt
     1981 cgccaccacc gacccgcgac tggtcaaggt tcgtggcgtc gtcgagaagc cgcgaccgga
     2041 ggacgcgccg agcaacctgg cgatcatcgg ccgctatgtg ctcacgccac gcatcttcga
     2101 gaagctcgag gagacaccgc gcggtgccgg cggcgagatc cagctgaccg acgcgatcat
     2161 ggcccttact tctgagcagg acgtttatgc ctatgcgttt gagggacgtc gatacgacgc
     2221 cggtactacc atgggttggc tgaaggcctc ggtcgagctc gcgctcgagc gagacgactt
     2281 tggtgccgag ttcaggcgcc atctcagcac gctcgatctg agctagatct gaaggccgct
     2341 ccggcgtcac cggagcgagt cgaaagccga caggggcccc tccgctccgg tcaccgcagc
     2401 aggcacaggc gcagagcagg ggatctctca tttggccgaa gtcggacagc tattagccgg
     2461 tcgctaccga ctcgtcgaac tgctcgggca gggcggcatg gcgaccatct accgcgcaca
     2521 tgacgagcag ctcggccgcg acgtcgc
//
LOCUS       DATLCO010000083         4669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_95145, whole genome shotgun sequence.
ACCESSION   DATLCO010000083 DATLCO010000000
VERSION     DATLCO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95145"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VMZ33_02115"
     CDS             complement(<1..263)
                     /locus_tag="VMZ33_02115"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HUR16057.1"
                     /translation="MTSQPAAVLDVPAVGTTMRALVKERAAPGAEIREVPIPDPGPGE
                     LLIKVLAASVCGTDVHIERWDPWAQENFAETPMTFGHEMAGVVV"
     gene            complement(306..827)
                     /locus_tag="VMZ33_02120"
     CDS             complement(306..827)
                     /locus_tag="VMZ33_02120"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HUR16058.1"
                     /translation="MTPSPAPTPVPTNRLGFTNTILGNQHVAGNASVSYPFCPPTSGD
                     HVNSVPRGPIPAQVYPATEVRTPTGWIHNLEHGYVAVLYRCPGGVIGGDGCPSQEEMN
                     LMQQWYDTAPAPTVASCPKKVLVARFDEMATRFAVVAWGRSMLLDNFELDMAKTFAEQ
                     WMEHDAVPEANSC"
     gene            775..1107
                     /locus_tag="VMZ33_02125"
     CDS             775..1107
                     /locus_tag="VMZ33_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16059.1"
                     /translation="MKPSRFVGTGVGAGEGVIAGEPEAAGEPDAVGESDPAGEPDAAG
                     EPDAAGEPDAAADGLGAAVALGTSVGLETGDGVTGSEGRGVSDSAGPGVIIAPHEKAA
                     VPAPWKKR"
     gene            complement(1546..1917)
                     /locus_tag="VMZ33_02130"
     CDS             complement(1546..1917)
                     /locus_tag="VMZ33_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16060.1"
                     /translation="MLQLFRDMPLGVRVVLVFGFLLLSGVGLTLPLIINEAVEAPVSP
                     LGLLWMLLLAYLVFTLTLILQRKQAAWLLSLGLASLSVPLAIVLFYWAGAAGAALGLA
                     VVIVLFVLLTRPRSRAWYTEP"
     gene            2088..3509
                     /locus_tag="VMZ33_02135"
     CDS             2088..3509
                     /locus_tag="VMZ33_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HUR16061.1"
                     /translation="MKLTGVVVALVLLIAACGGGGATSRPSTATSAAPSTAASAAPSQ
                     ATSAEPSAEPSEASPTEALPSEEAPTEEAPTEEPPSADPGASPSPTPPPAAEEGTLTL
                     WVDETRFPVVEALGAEFTAATDVPVAVYQVGFGDIRDRLQLYGPTGEGPDIVIGAHDW
                     LGQLASNGLVEPLDLSAVSDDIDPTALAAFTYDTGEGGQVYGLPYAAEAIALYYNPEL
                     VPTPPTTWDELIQMATDLQADGGLDQAFVLQEKDPYHSYPLLTANGGYIFGKNDDGTY
                     DPTDVGLDSEGGLAYGQMLSDMVEADLLRAGVNYDTMIQLFTTGESAMFMTGPWALTQ
                     IRDSGAPYAVAPIPSGSEDARPFVGVQGFMVSAFAPNKTTAMSFLTEYVATTETMQSL
                     FDADPRPSTWTPVAAATDDEDVQAFITSAANGDPLPAIPEMSAVWTDWTDALDLIFTG
                     AQDSDSALQDAAESIRGIIETGQ"
     gene            3561..>4669
                     /locus_tag="VMZ33_02140"
     CDS             3561..>4669
                     /locus_tag="VMZ33_02140"
                     /inference="COORDINATES: protein motif:HMM:NF027620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16062.1"
                     /translation="MSITGVLGRVIALCVIDAFALLLFYGLIGSDLWLGAVVLAAITI
                     LINYAFLTDRLLPVRWISPGLLLLTVMVVYPLLYTVYVALTNYGQGHVLTKEQVVTQF
                     ADDTFAGSSYTWTGFRSGDALLLLIIGPEGTQYVGDAQNATLTQIAAGDPRLGPIDPA
                     DSLPTTLDGYEKLPRLGTIRYLERLAVIRFAGQINGAEAEVRVRNVGVADVGQQKYTY
                     DTARDVLIDNETGIEYGAQDGFFTAPDGRRLNPGFSSFVGPNNFVRVLTDSRIQGPFL
                     GVFVWTFAFAGLTVLFAFAVGLGLALVLNDKQLPAKGVFRTLSIIPYTIPGFISALVW
                     VGLLNPLYGPINGVIEDLVGSSPRWFQDGNLAKVAL"
BASE COUNT          801 a         1532 c         1541 g          795 t
ORIGIN      
        1 acgacgaccc cggccatctc gtggccgaac gtcatcggcg tctcggcgaa gttttcctgt
       61 gcccaggggt cccagcgctc gatgtgaacg tctgtgccgc atacgctcgc ggcgagaacc
      121 ttgatgagca gctccccggg gccaggatcg ggtatgggca cttccctgat ctccgcgccg
      181 ggcgccgccc tttccttgac cagcgctcgc atcgtcgtac ccaccgctgg gacgtcgaga
      241 acggccgccg gctgcgaggt cacttgcgtg tagtcgtccg catggcgatc aggatgacag
      301 gcggctcaac aggagtttgc ctccggcaca gcgtcatgct ccatccattg ttccgcgaag
      361 gtcttcgcca tgtcgagttc gaagttgtcg aggagcatcg atcggcccca cgcaactaca
      421 gcgaaacgcg tggccatctc gtcgaagcgc gccaccagga cctttttcgg gcagctcgcg
      481 acggtcggcg ccggggccgt gtcgtaccac tgctgcatca ggttcatctc ttcctggctg
      541 gggcagccat cgccgccaat taccccgccc gggcatcggt acaggaccgc gacgtagcca
      601 tgctcgagat tgtggatcca gccggtcggc gtgcgtactt cggttgcggg gtacacctgc
      661 gcggggatcg gcccgcgcgg cacgctgttt acgtggtcgc ctgaggtcgg ggggcagaac
      721 gggtagctga cgctggcgtt gccggcgacg tgttgattgc cgaggatcgt gttggtgaag
      781 cccagccgat tcgtcgggac gggagtcggc gccggtgaag gcgtgatcgc tggcgaaccc
      841 gaagccgccg gagagcccga cgcggtcgga gagtctgacc cggccggaga gcccgacgcg
      901 gccggcgagc ccgatgcggc cggagagccg gacgcagcag cagatggact tggagctgcg
      961 gtggcgctgg gcacctcggt cgggctggag acgggcgacg gcgtcacggg gagcgagggt
     1021 cggggcgtta gcgactctgc cggcccgggg gtcatcatcg cgccgcacga gaaagcggca
     1081 gtccctgcgc cctggaagaa gaggtagccg atgagcaaaa cgccagctac ggctgcgccc
     1141 gtgatcgcca aactgcggta gcggtccatc ggagtctgct cgcgcgtgcg tcgggttcgg
     1201 gaagaccgag tgcgagagga ggagctcgac cgccggctgg agccgctcga cctgcggctc
     1261 gacgtggtcg gcggcacctc gtcgttcgtt gtgacgagca cgccgctggt cggacctgct
     1321 tcctcgggcg tgattggttc ggagaggcta tccggctcca gcacgtcggg agtcgactcc
     1381 aggggaaagg cctgcgccgt ctcgccgcgc acgggtcggc gcgatctttt tgccactgat
     1441 cagggatctc caatgcgaac cgccgcaaag ggcggtgatt gagtcagagt ggcgacaagc
     1501 ctacgtcacg agcggacgaa atgcctgatt tgagcgtggg aggtatcaag gctccgtgta
     1561 ccaggctcgc gacctggggc gtgtcagcag cacgaaaagc acgatcacaa cggccaggcc
     1621 gagggcggcg ccggctgctc cggcccagta gaagaggacg attgccagcg gcacgctgag
     1681 actggccaag ccgagcgaaa gaagccaggc cgcctgtttg cgctgcagga tgagcgtcag
     1741 tgtgaacacg aggtaggcga gtagcagcat ccacagcaga cccagcggcg atacgggtgc
     1801 ctcgacggcc tcgttgatga taagtgggag cgtcaggccg actcccgaca aaaggaggaa
     1861 gccgaataca aggacgacgc gaactccaag gggcatatcg cgaaacagct gcaacattct
     1921 gtgattctcc cactggtttc tggttggtag tctctggcct cgtgccgggg caacgtgccc
     1981 cacaggccta gcgcgctcga ccgtcgcgcg cgaacatgag agcgtcgagt tccaaggaaa
     2041 ggaacccaga cagcagagga ggaagcatcc gagctatgtc tcgtttcttg aagttgaccg
     2101 gcgtcgtggt ggcgctggtc ctgctcattg ccgcgtgcgg cggtggcggc gcgacgtcgc
     2161 gcccgagcac cgcgaccagc gcggcaccct caacagccgc atccgcggct ccttcacagg
     2221 cgaccagcgc cgagccctcg gcggagccgt cagaagcgag cccgaccgag gccttgccga
     2281 gcgaggaagc gccgacggaa gaggctccca ccgaggagcc gccgagcgcc gatccgggcg
     2341 cgtcgccgag cccgactccg ccgccggcgg ccgaggaagg caccctgacg ctgtgggtcg
     2401 atgagactcg tttccctgtc gtggaggcgc tcggcgcaga gttcaccgcc gcgaccgacg
     2461 tgcccgtggc cgtctaccag gtcggcttcg gtgacatccg cgaccgcctc cagctctacg
     2521 ggccgaccgg cgaaggcccg gacatcgtca tcggcgctca cgactggctt ggccagctgg
     2581 cgtcgaacgg cctcgttgag ccgctcgatc tcagcgctgt gtcggacgac atcgatccca
     2641 ccgcactcgc ggcgttcacg tacgacaccg gcgagggcgg ccaagtctat ggcttgccct
     2701 acgcggctga ggcgatcgcc ctgtactaca acccggagct ggtgccgact ccgcccacca
     2761 cgtgggacga gctcattcag atggcaaccg atcttcaggc tgacggcggg ctcgatcagg
     2821 cgttcgtcct ccaggagaag gacccgtatc acagctaccc actgctgacc gccaacggtg
     2881 gatatatctt cggcaagaac gacgacggga cgtacgaccc gacggacgtc ggtctcgaca
     2941 gcgagggcgg cctcgcctat gggcagatgc tgtccgacat ggtcgaggcc gacctgctcc
     3001 gcgcaggcgt gaactacgac acgatgatcc agctcttcac gactggtgag tcggcaatgt
     3061 tcatgaccgg cccgtgggcc ctcacccaga ttcgcgactc aggtgctccg tacgcggtcg
     3121 cgcccatccc ctctggctcc gaagacgcac gcccgttcgt gggcgtacag ggcttcatgg
     3181 tcagcgcgtt cgcgccgaat aagacgaccg ccatgtcgtt cctcaccgag tacgtcgcca
     3241 ccacggagac gatgcagtcc ctcttcgacg ccgatcctcg gccgtcgacg tggactcccg
     3301 tcgccgcagc gacggacgac gaggacgtcc aggcgttcat cacgagcgcc gcaaacggcg
     3361 atccactccc ggccatcccc gagatgtccg cggtgtggac cgactggacc gatgctctcg
     3421 acctgatctt caccggagcg caggactctg actccgcgtt gcaggacgcg gcggagagca
     3481 tccgcgggat catcgaaacc ggtcagtaac cgaccgtgac agcagggaca cagggggccc
     3541 ctcgaccgtc gagggccccc ttgtccatta ctggcgtcct tggccgcgtc atcgcgctgt
     3601 gcgtgatcga cgcgttcgcg ctcctgctct tctatggcct catcggcagt gacctgtggc
     3661 tcggcgccgt ggtcctcgct gcgatcacga tcctcattaa ctatgcgttc ctgaccgacc
     3721 ggcttctgcc ggtccgctgg atatcacctg ggcttctgct cctgaccgtg atggtcgtgt
     3781 acccgctgct gtacacggtc tacgtcgcgc tcaccaacta cggtcagggc cacgtcctga
     3841 ccaaggaaca ggtcgtcacg cagttcgccg acgacacctt cgcgggctcg tcttacacgt
     3901 ggacgggctt ccgctccggc gacgccctgt tgctgctcat catcggtccc gaggggaccc
     3961 agtacgtcgg tgatgcgcag aacgccacgc tcacgcagat cgcagcgggc gatccaaggc
     4021 tcgggccgat cgacccggcc gacagcctcc cgaccacact cgacggctac gagaagttgc
     4081 cgcgcctggg cacgatccgc tacctggaac ggctggccgt aatccgcttc gccgggcaga
     4141 tcaacggggc agaggccgaa gtacgcgtcc ggaacgttgg cgtcgccgac gtcggtcagc
     4201 agaagtacac atacgacacg gccagagacg tgctcatcga taacgagaca ggcatcgagt
     4261 acggcgcgca ggacggcttc tttactgcac ctgacggacg ccggctgaat ccaggtttct
     4321 cgtcgttcgt cgggcccaac aacttcgttc gggtactcac ggactcgcgc atccagggcc
     4381 ctttcctcgg cgtgttcgtt tggacgttcg cgtttgccgg actgactgtc ctgttcgcct
     4441 tcgcggtcgg tctcggcctg gcgctcgtcc tgaacgacaa gcagctgccg gccaagggcg
     4501 tgttccggac gctatccatc atcccgtaca cgattccggg cttcatctcg gcgcttgtct
     4561 gggtcggctt gctgaacccg ttatacgggc cgatcaacgg tgtcatcgag gatctcgtcg
     4621 gctcgtcgcc gcgctggttc caggacggca acctggcgaa ggtggcgct
//
LOCUS       DATLCO010000084         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5312051, whole genome shotgun sequence.
ACCESSION   DATLCO010000084 DATLCO010000000
VERSION     DATLCO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5312051"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..141
                     /locus_tag="VMZ33_02145"
     tRNA            66..141
                     /locus_tag="VMZ33_02145"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:His,seq:gtg)
     gene            complement(241..603)
                     /locus_tag="VMZ33_02150"
     CDS             complement(241..603)
                     /locus_tag="VMZ33_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16063.1"
                     /translation="MVRKVEEALAAWRDAQRRLDNAVDGDRDALQTEVERLRTLFHQL
                     SAEHMMDRIDALHEAEDRRQTATPSTEPFHTAARDEMHIAAEIWESARFSDEDTRSAK
                     PREDAWAEGEIARLKADD"
     gene            complement(907..1842)
                     /locus_tag="VMZ33_02155"
     CDS             complement(907..1842)
                     /locus_tag="VMZ33_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUR16064.1"
                     /translation="MTIGVRVRGLATAIAARPRLTAFLAAVSISFSGVLYLYSDTSPE
                     TASFFRGLYGLPILFLAAFAERKTARPMNRKAVGWAIVAGVLFAGDLIFWHHTIEYVG
                     AGLATVLGNLQVVIVGLATWLLFGERLNRRTLIALPIMLVGVALIAGVLTGEAYGIDP
                     PLGVFLGILTAFSYAGYLIVIRRIGRGRLALPVAISTTSTTIVSLVIGVAIGTMQFGI
                     SLNQHFWLLLLGVSAQALGYLFISYSLPRLPAVVTSIILLTQPVIAVFLSIILVNETP
                     SVEQLGGVALVIGGIALATVTVRRPSRRSVAVTAP"
     gene            1870..>2259
                     /locus_tag="VMZ33_02160"
     CDS             1870..>2259
                     /locus_tag="VMZ33_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16065.1"
                     /translation="MAAGRVEAPMNGKTPSLLLVGLLLAACSAGAARTTTGPGPSTPP
                     SATPATPTNPPSTNPPTAIAPIGSNPADPSGIDGRTFLSIGVKDGDQVRPLVPGTVIR
                     ISFNGNQISVQAGCNSFGGTYRLDGDTL"
BASE COUNT          427 a          747 c          711 g          374 t
ORIGIN      
        1 ttcaccacga aagcctagcg cgaacttcct gcgataattg cgggcgagga tcctcctcgc
       61 ccgcggtggc catagctcaa ccggcagagc atcggattgt ggatccgaag gttatgggtt
      121 cgacccccat tggccacccc aactcacgtc acgatcccgt gtcacgttgt gccaaccgcc
      181 gccccgatcg cgaagctgct gcggtagcgg cagcttcggg agccctggac ttcctagcct
      241 ttagtcgtcg gcctttagtc gggctatctc gccctcagcc caggcatcct cgcgcggctt
      301 ggccgaacgt gtgtcctcgt cgctgaaccg cgccgactcc cagatctctg ccgcgatatg
      361 catctcgtcg cgcgccgcgg tgtgaaacgg ctcagtcgag ggcgtggccg tttggcgacg
      421 gtcttcagct tcatggagcg cgtctatccg gtccatcatg tgctctgccg acagctgatg
      481 gaagagcgtg cgtagccttt cgacctcagt ctgcaaggcg tcacgatcgc cgtccaccgc
      541 attgtcgagc cgtcgctgcg cgtcgcgcca cgcggcgagt gcctcttcaa cttttcgaac
      601 catgatgccc ccgtccggat tgagcgggga ccgctcaata aagcggagac ccagcgccaa
      661 aaagcgaatg tacggaggtg aggaacggct ggcattacgg tccgtaatga cctagctcct
      721 cgagccgtgc ctggtcgcca gacagacgtt cttactcggt ttcttggagg gtcccgccgt
      781 gactttcctg atgcagtcac gcgatgccgg tacgaacgcc cgcgagtgga tccgaaggtt
      841 atgggttcga ccccattggc cactccaacg agtgaaccgc tgactgtggg actgacgacg
      901 tggcgtctat ggcgcggtga cggccacaga ccttcgggat gggcgacgca cggtgacggt
      961 tgccagcgcg atgcccccga tcaccagtgc aacgccgccg agctgctcca cggacggcgt
     1021 ctcgttcacc aggatgatcg acaggaagac ggcgatgacc ggctgggtga gcaggatgat
     1081 cgacgtcacg accgccggaa gccgcggcag tgagtacgag atgaataggt agcccagcgc
     1141 ctgggcgctg accccgagca gcagaagcca gaaatgctgg ttgagcgaaa tgccgaactg
     1201 catcgttccg attgccacgc cgataaccaa cgagacgatg gtcgtcgacg ttgtggagat
     1261 cgccaccggc agcgctaggc ggccgcgtcc gatccgccgg atgacaatga gatagccggc
     1321 gtacgagaag gcggtcagga tgcccaggaa aacgccgagc ggcgggtcga tgccatacgc
     1381 ctcgccggtc agtacgcccg cgatgagcgc gacgccgacc agcatgatcg gcagcgcgat
     1441 gagcgtgcgc cggttgaggc gctccccgaa cagcagccac gtggccagac ccacgatcac
     1501 cacctgcagg ttgcccagca cagtggcgag gccggcgccg acgtactcga tggtgtggtg
     1561 ccagaagatg aggtcacccg cgaacagaac gccggcgacg atggcccagc caacggcttt
     1621 gcggttcatc ggccgggccg tctttcgttc ggcgaacgcc gccaggaaca ggatcggcag
     1681 gccatagagg ccacggaaga agctggccgt ctcgggcgac gtgtcggagt agaggtacag
     1741 caccccggag aacgagatgg acaccgccgc gaggaacgcg gtcaggcgcg gacgggcagc
     1801 aatggcggtg gccagacctc tcactcggac gccgattgtc acgcacgaat tcgaaccctg
     1861 cggaacccga tggccgcggg tcgcgtcgag gcgcctatga acggcaagac cccttccctc
     1921 ctccttgttg gccttcttct cgcggcctgc agcgccggcg cagcgcgaac caccacgggt
     1981 ccgggaccct cgacgccgcc gagcgccacg ccagctacgc cgacaaatcc gccaagtacc
     2041 aacccgccca ccgccatcgc gcccataggc agcaaccccg ccgatccgtc gggtatcgac
     2101 ggccgtacct tcctttccat cggggtcaag gacggcgatc aggtccgtcc tttggttccc
     2161 ggcacggtca tccgcatcag cttcaacggc aaccagatct cagtgcaggc cggctgcaac
     2221 tcgttcggcg gcacgtaccg ccttgatggc gacacgctt
//
LOCUS       DATLCO010000085         3448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_840609, whole genome shotgun sequence.
ACCESSION   DATLCO010000085 DATLCO010000000
VERSION     DATLCO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840609"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..646
                     /locus_tag="VMZ33_02165"
     CDS             206..646
                     /locus_tag="VMZ33_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR16066.1"
                     /translation="MSVQNGRAFNEWLRTQLKAKKMSQRQLAQQSGVDHSTISRLVRG
                     DRTPSLGTATKLARGLRELRDETETPHYFGVVASSTSHPTARVEYALRSDDLLAEPQV
                     RQVMEYYLALRMRRLAATRQGPQLMRPNGADTRPSLAGRMPSRN"
     gene            complement(726..1823)
                     /locus_tag="VMZ33_02170"
     CDS             complement(726..1823)
                     /locus_tag="VMZ33_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HUR16067.1"
                     /translation="MTGNDQGGPIADEPLVDYPLVHEESAPPQPLSKRLRDPRTIISI
                     VLPLFLLLLIAFNLGGLDFNQLGATILAANPMLLLAAFAIYYIGFPLRGYRWQKLLTS
                     AGSPVTLRDSTEIIFISWLVNCLVPAKLGDVYRAWLLKANYPVSLSRTFGTVFIERIF
                     DLFAIAILGLAAGFWSFRGGLPTEVQVVFAIGLVVILLLALALYTVRNFGRRALVRLP
                     IPAKGKVLEFYDRFEEGVFAINPRQIPALGIVTALIWATEAMRLLLVINALNFTDVHL
                     GISGAFFVALAASLLTAIPFTPAGLGIVELGVVGILTVIYGIDPTNAAAITLVDRAIS
                     VLSIIVFGGIAYVVSSKTKARKQPAAGPASS"
     gene            complement(1820..2557)
                     /locus_tag="VMZ33_02175"
     CDS             complement(1820..2557)
                     /locus_tag="VMZ33_02175"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HUR16068.1"
                     /translation="MSESAPPHNAPPNERQRSFADLHCHTNASFDSLSRPEAVVRAAV
                     ERGITHLAITDHDRIEGALAARDFAPNGLTVIIGQEVRTSAGDLIALFVERPIRPGQS
                     PADASEEIRAQGGLVGLAHPFDRFRAGVGRKGWEDELERAMPFIDYVEIWNARLFMGS
                     GNAEAAKYASEHNLAGVAVSDAHTVIEVGVAYTILEGPLATPDEFRAALLNGQLVTGH
                     GSRVVRLAMPVTKLIQRLRGNRRVAAA"
     gene            complement(2554..>3448)
                     /locus_tag="VMZ33_02180"
     CDS             complement(2554..>3448)
                     /locus_tag="VMZ33_02180"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HUR16069.1"
                     /translation="DDNHIVAVGEEAREMIGRTPGNITAIRPMKDGVIADYVITEAML
                     RYFIKKAGRSFTKPDVMISVPSGVTSVEKRAVRDAALKAGAGAAYLIEEPLAAAIGAN
                     VPISGPSGNLIIDIGGGTSEIAVISLGGIVVSRSVRVGGNRFDEAISNYVRKKYNVMI
                     GDRTAEEVKIQIGTALPLERELHMEVRGRDLIAGLPRTVPITSSEVMEALEQPLQQIV
                     QAVRGVLEETPPELSSDIIDKGMVMSGGGALLRNIDKLLTQVTGVPCHVAENALNCVA
                     LGTGLALEHFDVFKKSLVQRI"
BASE COUNT          639 a         1086 c         1145 g          578 t
ORIGIN      
        1 gatctcccgc cggaagcgtt cacgctgcgc agctcgaggt cgcgcagcgg tccgccccag
       61 gtctaacccg gtcgtgcccg aagcgaccgt accaagggcc tatatcgacc agcgggggcg
      121 caccggaaca tgcccttgtc gcacacagag cggccgttcc agcaaggccc aacgtcgcgc
      181 ggcctacgga gaaccgaagg gtccgatgag cgtccagaat gggcgggcct tcaacgaatg
      241 gttgaggact caactcaagg cgaagaagat gtcccagcgt cagctggctc aacagtccgg
      301 cgtcgatcat tcgacgatct cgcgcctcgt ccgcggcgat cgaaccccgt cgctgggcac
      361 ggcaacgaag cttgcccgtg gcctgcgcga gttgcgtgac gagaccgaga cgccccacta
      421 ctttggcgta gtggcgtcgt cgacgagcca ccccacggca cgcgtcgagt acgccctccg
      481 atccgacgat ctcctggctg agccacaggt tcgccaggtc atggagtact acctggcgct
      541 gcggatgcgc cgcctcgcag ctacccgaca gggcccgcag ctgatgcgtc cgaacggcgc
      601 ggatactcga ccgtcgctcg ccggccggat gcccagccga aactagccga tcaggcgccc
      661 gacgtctgta acggacgtgc cccgtgagac aaccagatgc cggtcgacag accggcatca
      721 ttgattcagg agctggcagg ccctgcggct ggctgcttcc gagccttggt cttggatgag
      781 accacgtaag cgatgccacc gaagacgatg atcgacagga cgctgatggc gcggtccaca
      841 agcgtgattg ccgccgcgtt cgtcggatcg atgccgtaga tcacggtcag gatgccgaca
      901 acgcccaact cgacgatgcc gaggccagcc ggcgtgaacg ggatcgccgt gaggagtgac
      961 gctgcaagag caacgaaaaa ggcgccgctg atgccgagat ggacgtccgt gaagttcagc
     1021 gcgttgatga ccagcaaaag gcgcattgcc tcggtcgccc agatgagcgc cgtgacaatg
     1081 cccagcgccg gtatctggcg cgggtttatg gcaaagacgc cttcctcgaa gcggtcgtag
     1141 aactcgagta ccttgccctt ggcaggaatg ggcagccgga cgagcgcgcg acggccgaag
     1201 ttgcgaacgg tgtagagggc cagcgccagc agcagaatga cgaccagacc gatggcaaat
     1261 acgacctgga cctctgtcgg gagcccgccc cgaaatgacc agaaaccagc cgccaatccc
     1321 aggatcgcga tcgcgaacag gtcgaagatg cgctcgatga aaacggtgcc gaatgtgcgg
     1381 ctcagcgaga ccggatagtt ggccttgagg agccaggcgc ggtagacgtc gccgagcttc
     1441 gcgggcacta ggcagttgac gagccagctg atgaagatga tttctgtcga gtcgcgcagg
     1501 gtgaccggag aacccgcgct cgtgagcagc ttctgccagc ggtatccacg gagcggaaag
     1561 ccgatgtaat agatggcaaa agcagccagg agcagcatcg ggttggcggc caggatggtg
     1621 gcgcccagct ggttgaagtc gaggccgccc aggttgaacg cgatgagcag cagcaggaag
     1681 agcggcagga cgatggagat gatcgtccgc gggtcgcgca gcctcttcga cagcggctga
     1741 ggcggagcgg actcctcgtg tacaaggggg tagtcgacga ggggctcgtc cgcgatcggt
     1801 ccgccctggt cgttgccggt catgcagccg ctactctgcg gtttccgcgg agcctttgta
     1861 tgagcttggt cacaggcatc gccagtcgga cgacgcgcga cccgtgtcct gtgacgagtt
     1921 gaccgttcag gagtgcggcc cggaactcat cgggcgtggc cagcggcccc tcgaggatcg
     1981 tgtacgcaac cccgacctca atgacggtgt gggcgtctga gacagctacg cccgcgaggt
     2041 tgtgctcgct ggcgtatttt gcggcctcgg cgttgccact tcccatgaaa agccgcgcgt
     2101 tccagatctc cacgtagtcg atgaacggca tcgcgcgctc gagctcatcc tcccagcctt
     2161 tgcggccaac cccggcccgg aagcgatcga aggggtgcgc gaggccgacc aacccaccct
     2221 gggcccgtat ctcctcggag gcgtcggcgg gcgactgacc aggacggatc ggccgctcga
     2281 cgaagagtgc gatcagatcg ccggcgctgg tgcggacctc ctgcccgatg atcactgtca
     2341 acccgttcgg ggcgaaatcc cgcgcggcga gtgctccttc gatcctgtcg tgatccgtga
     2401 tcgccagatg cgtgatgccg cgctcgaccg ccgcgcggac gacggcctcg ggccgactga
     2461 gcgaatcgaa tgacgcgttg gtgtggcaat gcaggtcggc gaacgagcgt tggcgctcgt
     2521 tcggcggggc gttgtgcggc ggcgcggatt cgctcagatt cgttggacga gcgacttctt
     2581 gaacacgtca aagtgctcga gcgcaaggcc cgtaccaagc gccacgcaat tgagcgcgtt
     2641 ctcggcgacg tgacacggca cgccagtcac ctgggtgagc agcttgtcga tgttgcgcaa
     2701 gagtgcgccg ccaccgctca tgaccatgcc cttgtcgatg atgtcgctcg aaagctcggg
     2761 cggcgtctcc tcgagaacgc cgcgcacggc ctggacgatc tgctggagcg gctgctcgag
     2821 cgcctccatc acctcagacg aagtgatcgg cacggtccgc ggcaagcctg cgatcaggtc
     2881 ccgaccgcga acttccatgt gcagctcacg ctccagcggc agcgcggtgc cgatctggat
     2941 cttgacctct tccgccgtcc ggtcgccgat catcacgttg tacttcttgc ggacgtagtt
     3001 gctgatggcc tcgtcaaagc ggttgccacc gacgcgcact gagcggctga cgacgatccc
     3061 gccgagcgag atcacggcga tctcagaggt gccaccgccg atgtcgatga tcaggttgcc
     3121 tgacggaccg ctgatgggca cgttcgcccc aatggcggcg gcaaggggct cctcgatcag
     3181 gtacgccgcg ccggcgcctg ccttaagggc ggcgtctcta acggcgcgtt tctcgacgct
     3241 ggtcacgccg gaggggacgc tgatcatcac gtcgggcttg gtgaatgagc gaccggcctt
     3301 cttgatgaag tagcgcagca tcgcctcggt gatcacatag tcggcgatga ccccgtcctt
     3361 catgggccgg atcgcggtga tgttgcccgg ggtgcggccg atcatctcgc gtgcctcttc
     3421 gccgacggca actatgtggt tgtcatcg
//
LOCUS       DATLCO010000086         2268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2332888, whole genome shotgun sequence.
ACCESSION   DATLCO010000086 DATLCO010000000
VERSION     DATLCO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332888"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VMZ33_02185"
     CDS             <1..738
                     /locus_tag="VMZ33_02185"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUR16070.1"
                     /translation="IQRANDVAPRYFGRLPIAGCEVRPVEPYQEAEAPPAFYFPPAPD
                     GSRGGIYYVNTYQPENRPLYRLATTTYHEATPGHHFQIAMEGELTDMPDFRRFGARLT
                     GAAYAEGWGLYSERLADEMDLFESAWERLGMLDAQAWRAARLVVDTGLHAFQWPRQKS
                     IDLLLDIGLSQLEAETETDRYITWPGQALAYMTGQREIMALRHQLEARDGDRFDLVKF
                     HDELLGHGTLPLATLRKHLPGWVTPRS"
     gene            814..1764
                     /locus_tag="VMZ33_02190"
     CDS             814..1764
                     /locus_tag="VMZ33_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HUR16071.1"
                     /translation="MDIQRLINDLAASGALRVIIQIALIVLATFVAMRFAHVLVSGTL
                     NRLFQREVVEGTAQELSAVELERRRKTLDHLAYRSLRVIILVIAFLMVLHVLDLNIGP
                     AIAGLGVVGLSLSLGAQHLVRDYVAGAFILIENQYSKGDVVTIAAVSGTVEDVSLRRT
                     TLRDHDGTVHYVPHGLIQTASNHTRNWAAIEIDLPITYEQDLAALTEAVAVAGKRLAT
                     DPKWRAAIFEAPRVASVERLAETGLLVRVSASVAALHRYALPGVARGLVLEECTRRGI
                     VIGWREAPTPSADSPARPAKADLSAEAAAPVVMPPAEADG"
     gene            1774..>2268
                     /locus_tag="VMZ33_02195"
     CDS             1774..>2268
                     /locus_tag="VMZ33_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HUR16072.1"
                     /translation="MTDTSLPGSLSEPELENLLAETRRFPPGAEFMAQANATADLYEE
                     AERDPEAFWARLARERLAWDTPFETTLEWEPPFSKWFLGGKINASYNCVDRHVENGLG
                     DKVAYHWIGEPGDTRTITYADLQREVSKAANALKELGVKSGDVVAIYMPMIPELPIAM
                     LACAR"
BASE COUNT          374 a          791 c          735 g          368 t
ORIGIN      
        1 atacagcgtg ccaatgacgt ggcgccgcgc tacttcggtc gactgccgat cgccggctgc
       61 gaggtccgcc cggtcgagcc ctaccaggag gcggaggcac cgcccgcctt ctacttcccg
      121 cccgcgcccg acgggtcgcg aggcggcatc tactacgtca acacgtacca gcccgagaac
      181 cgccctctct accgactggc cacgacgacg tatcacgaag cgactccggg ccatcacttc
      241 cagatcgcca tggaaggcga gctgaccgat atgcccgatt tccggcgctt cggcgctcgc
      301 ctcaccggcg ccgcgtacgc cgagggctgg gggctctaca gcgagcgtct ggcggacgag
      361 atggacctct tcgagagtgc atgggagcga ctgggcatgc ttgacgcgca ggcgtggcgc
      421 gccgcccgac tcgtcgtcga cactgggctg cacgctttcc aatggccccg ccagaagtcg
      481 atcgacctgc tcctcgacat cggcctgtcg cagctggagg cggagaccga aaccgaccgc
      541 tacatcacgt ggcccggtca ggccctcgcc tacatgaccg gccagcgcga gatcatggcg
      601 cttcgtcacc agctcgaggc gcgcgacggc gatcgtttcg acctggtgaa gttccacgac
      661 gagctcctcg gccacgggac attgccgctg gctacgctgc gcaagcacct gcctggctgg
      721 gtcacgcccc gctcctgacc ggacctcggc tgccgcggcc gaagttaccg aacggcaccc
      781 gtccgcgccc cctcgctcga taacctagtg gcggtggaca ttcagaggct gatcaacgac
      841 ctggctgcct ccggcgcact gcgcgtgatc atccagattg cgctgatagt cctggcgacg
      901 ttcgtggcga tgcgtttcgc gcacgtgctc gtgtcaggca cgctgaaccg gctcttccaa
      961 cgcgaagtgg tcgagggaac cgcccaggag ctcagcgccg tcgagctcga gcgtcgccga
     1021 aagacgctcg accacctcgc ctacaggtcg ctgcgcgtga tcatcctggt gattgccttc
     1081 ctgatggtcc tccatgtgct cgacctcaac atcggaccag cgatcgccgg acttggcgtc
     1141 gttgggctgt cgctgagcct gggcgcgcag cacctcgtcc gtgactacgt cgccggggcg
     1201 ttcatcctca tcgagaacca gtattcgaag ggtgacgtcg tgacgattgc cgcggtgagc
     1261 ggcacggttg aggacgtgag tctccggcgc acgacgctgc gcgatcacga cgggacggtc
     1321 cactacgttc cgcacggtct catccagacg gcgagcaacc acacgcgcaa ctgggcagcg
     1381 atcgagatcg acctgccgat cacctacgaa caggatcttg cggcgttgac tgaggcggtt
     1441 gccgtagctg gcaagcggct ggcgacggac cccaagtggc gcgccgcgat cttcgaggcg
     1501 ccgcgcgtcg cgagcgtcga gcggctggcc gagacgggcc ttctggttcg ggtatcggcg
     1561 tcggtcgcgg ctctgcaccg gtacgcgctc cccggagtcg cccgtggcct cgtcctcgag
     1621 gagtgcacgc gccgcggcat cgtcatcggt tggcgggagg cgcccacacc gtcggccgac
     1681 tctccggcca gaccggccaa ggcggatttg tcggccgagg cagctgcgcc cgtggtcatg
     1741 ccgcctgccg aggccgacgg ctagcatcgg cgaatgaccg acacgtcact gccgggctcc
     1801 ttgagcgagc ctgagcttga aaaccttctg gcggagaccc gtcgcttccc gccgggtgcc
     1861 gagttcatgg cccaggccaa cgccacggcc gacctgtacg aggaagccga gcgcgacccc
     1921 gaagccttct gggcgaggct tgcccgcgag cggctcgcct gggacacccc cttcgagacc
     1981 actctcgagt gggagccacc gttctcgaag tggttcctcg gcggcaagat caacgcctcg
     2041 tacaactgcg tcgatcggca cgtcgagaac gggctcggcg acaaggtcgc gtaccactgg
     2101 atcggcgagc cgggcgacac gcggaccatc acctacgccg atctccagcg cgaggtgtcg
     2161 aaagccgcca acgcgctgaa ggagctcggc gtgaagtccg gcgacgttgt cgcgatctac
     2221 atgccgatga tccccgagct gcccatcgcg atgctcgcct gcgcaaga
//
LOCUS       DATLCO010000087         2984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1588232, whole genome shotgun sequence.
ACCESSION   DATLCO010000087 DATLCO010000000
VERSION     DATLCO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588232"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..618
                     /locus_tag="VMZ33_02200"
     CDS             76..618
                     /locus_tag="VMZ33_02200"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HUR16073.1"
                     /translation="MRSGSCGRFPRCGSRTDVDLAAVLKVIHALVGIVLVIGLVGRWV
                     TLVYAARLDDIRVLRGVLGLGVIFERMVIASSLFVLVLGLLTSIAQDRPFLGPLQGAP
                     VDWLFVSLVLYVSVSFLVPLVFLPRGRVFQGVLDDATKRGEVTDELRLAFRDRVVVSA
                     HVYEMVAVTIVLVLMIAKPF"
     gene            696..1151
                     /locus_tag="VMZ33_02205"
     CDS             696..1151
                     /locus_tag="VMZ33_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HUR16074.1"
                     /translation="MEAAATFLGVRPRSVAETTRRLQHLGYPPPLVDEVVRRLIEMEY
                     LDDAAFAKEWLESRDRARPRGLSVLRRELALKGVDRTVVEEALIERANGADDPERAAA
                     FALLQRRRMALDRELDPSKRRQKAYALLARNGFDPETCRAVSASMVASQ"
     gene            1157..1594
                     /locus_tag="VMZ33_02210"
     CDS             1157..1594
                     /locus_tag="VMZ33_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HUR16075.1"
                     /translation="MTSTARWLLLLYVLGLGLLVFLPFGRPMDLGDRVNLDPFTTIER
                     ALQLGPRSSSYRLLIGNIVAFVPFGILLPLAFRRLFIVGGFLIAVALSVVIELGQLAV
                     STYLGYAYRSTDVDDVILNVVGATLGLIVAGILRLSQAIRETS"
     gene            complement(1591..>2984)
                     /locus_tag="VMZ33_02215"
     CDS             complement(1591..>2984)
                     /locus_tag="VMZ33_02215"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HUR16076.1"
                     /translation="TGLPNKALFMDRLERVIARRPRRPQGFVAVLFIDIDDFKVVNDT
                     LGHAAGDLLLAEVGRRLTSSLGPADTPARFGGDEFTILLQDLSGPDEALRAAARIGEN
                     ASEPVRIGERDVTVTVSVGISTGSGENLRATELVSQADIAMYRAKENGKARAELFDAE
                     MGAEAWRRLNLQSDLRHAIERHELAVHFQPILDLKTGELSQVEALVRWVHPERGLLLP
                     AEFVPFAEMTGMISHIDRFVLEESCRQFALWQRRFPWARQLQVAVNMSPRELRQPDLA
                     ADVARVLSENGVAPSRLVLEITESSSLIGSDTVRAVLDRLHRLGVQLVVDDFGVGYSG
                     LDHFKSFKVDGLKIDRTFVAGIPHQPEDTAIVTATLAFARALGLSVVAEGIETDRQLS
                     ALSALGCERGQGRLLSPPVRAGAMATMLRSRRTLLPQTRRPVPATRSSVPARPTRNRP
                     TGEPRGVAPPVFG"
BASE COUNT          491 a          924 c          971 g          598 t
ORIGIN      
        1 caatcgtccg cgctgagttc gaccactggt ttggtgatgt cggaccttgg gacgaccgcc
       61 tcgacctcgc ggccaatgag atctggcagc tgtggcaggt ttccccgttg cgggtcacgc
      121 actgacgtgg atctagccgc cgttctgaaa gtcattcacg cgcttgtggg gatcgttttg
      181 gtgattgggc tggttgggcg gtgggtgacg ctcgtgtacg cggcgcggct ggacgacatt
      241 cgggtactgc gtggtgtgct cggcctgggg gtgatcttcg agcggatggt catcgccagt
      301 tcgctattcg tgctcgtgct cggcttgctc acttcaatcg cgcaggacag gccattcctc
      361 ggaccgctcc agggcgctcc ggtggattgg ctgttcgttt cgctggtgct ctacgtcagc
      421 gtctcgttcc tggtgccgct cgtttttctg ccaagaggcc gcgtctttca gggtgtactg
      481 gacgacgcga ctaagcgcgg tgaggtcacg gacgagctgc ggctcgcctt tcgtgaccgg
      541 gtagtggtga gtgcgcatgt ctacgagatg gtcgccgtca cgatcgtgct cgtgctcatg
      601 atcgccaaac ccttctgatg gggggcagac gcacagcaga atcacgacaa gccctcaggg
      661 acttgcgcgc agagctgcgc gatcctgccc ctgtgatgga agctgccgcg acctttctcg
      721 gtgtccggcc acgatctgta gcggaaacaa ctcgtcgcct gcagcatctc ggctatccgc
      781 cgccgctggt ggacgaagtc gtcaggcgcc tgatcgagat ggagtacctc gacgacgccg
      841 cgttcgcaaa ggaatggctg gagagccgcg accgagcgcg cccacgcgga ttgagcgtgc
      901 tccgacggga gctcgcattg aagggcgtcg atcggacagt cgtcgaggag gcgctgattg
      961 agcgcgccaa tggtgctgat gacccggaga gggccgcggc cttcgcgctg ctccagcgcc
     1021 gacggatggc gctggatcgg gagctcgacc caagcaagcg tagacagaag gcgtacgcgt
     1081 tgctggcgcg caacggcttt gatcccgaaa catgtcgagc ggtcagcgcg tcaatggtgg
     1141 cgtcgcaatg aactccatga cttccactgc ccgctggttg ctgctgctct atgtgctcgg
     1201 cctggggctg ctcgtgttcc tgccatttgg gcgtccgatg gatctcggtg accgggtcaa
     1261 cctcgacccg ttcacaacca tcgagcgtgc cttgcaactc ggccccaggt cgtcgtcgta
     1321 ccgacttttg atcgggaaca tcgttgcctt cgtgccgttt ggcatcctct tgccgctggc
     1381 gtttcggcgg ctcttcatcg tcggcggttt tctcatcgca gtcgcgttat cggtcgtgat
     1441 cgagctcggg cagctcgctg tctcgaccta tctcggctat gcctaccgct ccactgacgt
     1501 tgacgacgtg atcctcaacg tcgtcggcgc gacgttgggc ctgatcgtgg ctgggatctt
     1561 gagactcagc caggcgatca gggagaccag ctagccgaag acgggagggg ccacaccgcg
     1621 aggctcaccc gtggggcggt tacgagttgg cctcgccgga acgctgctgc gagtggcggg
     1681 cacaggcctt ctggtctgcg gcagcaaagt ccgtctgctc cgcaacattg tggccattgc
     1741 gcctgcccgg acgggtggcg acaggagcct gccctgaccg cgctcgcatc cgagagcact
     1801 cagcgctgaa agctgtctgt cagtctcgat cccctcggca accaccgaca acccgagcgc
     1861 gcgagcgaat gcgagagtgg cggtcacgat ggccgtatct tccggttgat gaggaattcc
     1921 agccacgaac gttcggtcga tcttcaggcc gtccaccttg aagctcttga agtggtcaag
     1981 gccgctgtat ccgaccccga agtcgtcgac tacgagctga acgccgagtc tgtgtaggcg
     2041 atccaagacc gcacggactg tgtcggagcc gatcagggag gagctttcgg tgatctccag
     2101 caccagccgc gacggcgcca ctccattttc gctgagaact cgtgccacgt cagccgcgag
     2161 gtcgggctgg cgcagctccc gcggcgacat gttgacggcc acttgcagct gccgagccca
     2221 cgggaagcgg cgctgccaca gggcgaattg acggcatgac tcctcgagca cgaaacgatc
     2281 gatatggctg atcatgccgg tcatctcggc gaaggggacg aactccgctg gaaggagtag
     2341 gccccgctcc ggatggaccc atcgaaccag cgcctcgacc tgactgagct caccggtttt
     2401 caggtcaagg atcggctgga aatggacggc aagctcgtgc cgttcaatgg catgtcggag
     2461 gtcgctctgg agattgaggc ggcgccaggc ttctgcgccc atctcagcgt caaacagctc
     2521 tgcccgagcc ttgccgttct ccttcgcgcg gtacatggcg atatccgcct ggctcaccag
     2581 ctcagtagcg cggaggttct caccgctgcc agtgctgatg cccacgctca cggtcaccgt
     2641 tacgtcgcgc tctccgatgc ggacgggttc ggaggcgttc tccccgatcc gcgctgcagc
     2701 gcgcaaggct tcgtccggac cgctcaggtc ttggagcagg atcgtgaact cgtcgccacc
     2761 gaatctcgcg ggcgtatccg ccggcccgag cgatgacgtc agtcgccggc ccacctcggc
     2821 cagcaagagg tcgccggccg cgtggccgag tgtgtcgttg acgaccttga aatcgtcgat
     2881 atcgatgaac aagacggcga cgaacccctg aggccgacga ggtcggcggg cgatcactcg
     2941 ctccagtcga tccatgaaga gggccttgtt cggcaggccc gtca
//
LOCUS       DATLCO010000088         2536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8247250, whole genome shotgun sequence.
ACCESSION   DATLCO010000088 DATLCO010000000
VERSION     DATLCO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8247250"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VMZ33_02220"
     CDS             <1..374
                     /locus_tag="VMZ33_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16077.1"
                     /translation="DLAGHEQDSNRYRRVLENVDRWIPRILERLGSEGVLFVAADHGN
                     DPMLGTSRHTREYVPILVAGQAIGPLELGTRTSLSDIAATIAELLGAAPVPAGRSFAR
                     LLGPVSAHALETRETAPSMMS"
     gene            371..1474
                     /locus_tag="VMZ33_02225"
     CDS             371..1474
                     /locus_tag="VMZ33_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HUR16078.1"
                     /translation="MTSMAEPTIVNVQLSRYSSDIHGQPAALRALADRLALPGAFAPI
                     TARWQELGRPSIVITGMGASLYAAEAAVAQVPLSVARVQVIATAQLLEQPRHLDDMLV
                     IAVSQSGESVEIRELLDAVAADRLAAITNSADSSLGRSVAGSLVLALPGDRSVAIKTY
                     TGTLAAIVLMLQSLNQEALAVDDIRDAAEMIETSLPSWTVAASSAAGRIGDARAIAVL
                     GWGAMAATARAAALVFKEAARTPSEGMHADDFRHGSVEVVDSRFGALVFANSSSTRSD
                     PWLAVAEITRANGHVVVIGGRLEGEASPGTVTHIATTPRPGIAALLTDIVPVQLLCAR
                     LAELSGFEAGEFRNTTPVITERPSGRLVSAPRQ"
     gene            1508..>2536
                     /locus_tag="VMZ33_02230"
     CDS             1508..>2536
                     /locus_tag="VMZ33_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUR16079.1"
                     /translation="MDMKRAFGLVCATALVAVACGGPAVTQRASTGASQAPASTAASS
                     APSTAASTEPSAAASAQATPGVDLGHCSDAAADLSATSTGPNGEESTSASDIPDLTDE
                     QVAAANAAGYKWAYLPSGASTWFNAVGEGAKAEAERLGLTLTVTADSNFDPAKQAADV
                     ESAMATDPDIIVTLPVDPTQGAEAFRPAVDAGVKLVFNDNGVDGFTAGEEYVAIVTGD
                     HFGMGDAAADLMSQALPDGGPIGVIFHDADFYVTNNRDCRFVAAVEQNYPELEIVATS
                     GMTEEGATESITSAMLTQHPEIKGIYVTWSAAATGALAALRGADRSDVKVVVHDLDAA
                     NDLDMAQNG"
BASE COUNT          438 a          869 c          821 g          408 t
ORIGIN      
        1 cggacctggc cggccacgag caggactcga accgctatcg ccgtgtgctg gaaaacgtcg
       61 accgctggat tccgcgcatc ctcgaacgac ttggtagcga gggcgtgctc ttcgtagctg
      121 ccgatcatgg aaacgatccg atgctgggaa cgagccggca cacgcgcgaa tacgtgccca
      181 tcctcgtggc cggacaggcg atcgggccgc tcgagctggg cacgcgcaca tcactgagcg
      241 acattgcggc gacgatcgct gagttgctgg gtgcggcgcc tgtccccgcc ggccgaagtt
      301 tcgccaggct gctcggccca gtttctgcac atgcgttgga aacgcgcgaa acagcaccta
      361 gtatgatgtc atgacaagta tggccgaacc gacgatcgtc aacgttcagc tgagccggta
      421 ctcgagcgac atccatggcc agccagcggc cctccgcgcg ctcgctgacc gtcttgcgct
      481 gcccggtgcg ttcgctccaa tcacggcgcg ctggcaggag ctcggacgac cgtccatcgt
      541 catcaccggt atgggcgcat cgctttacgc cgcggaggcg gccgtcgcgc aggtgccgtt
      601 gtccgttgcc cgcgtccagg tgatcgcgac agcgcagctg ttagagcagc cacgccacct
      661 cgacgatatg ctcgtcatcg ccgtctcaca atccggcgaa agcgtcgaga tccgcgagct
      721 cttggacgcc gtcgccgcgg accgcctcgc agcgatcacc aacagcgccg atagctcgct
      781 cggccgctcc gtggctggaa gcctcgtcct ggccttgccc ggcgatcgat ctgtggccat
      841 caagacctac accggcacgc ttgccgccat cgttctgatg ctccagtcgc tgaatcaaga
      901 ggcgctcgcg gtcgacgaca tccgggacgc ggccgagatg atcgagacga gcctgccgag
      961 ttggaccgtg gccgcgtcga gcgcggccgg gcggattggc gatgcacgcg caattgcggt
     1021 tctgggctgg ggcgccatgg cagcgaccgc gcgtgcggcc gcgctcgtct tcaaggaggc
     1081 ggcgcggaca ccatctgaag gcatgcatgc cgacgacttc cggcacgggt cggttgaggt
     1141 cgtcgacagc cgtttcggcg cacttgtgtt cgcaaactcg tcatccaccc gcagcgaccc
     1201 gtggctggcg gtcgccgaga tcactcgggc aaacggccac gtagtcgtta tcggcgggcg
     1261 cctcgaaggc gaggcgtcac cgggcaccgt cactcacatt gccaccacgc cgcgtccggg
     1321 gatcgcagca ctgctgacag acatcgtccc cgttcagctt ctctgtgcgc gcctcgctga
     1381 gctctcgggt ttcgaggccg gcgagtttcg caataccacc cctgttatca ccgagcggcc
     1441 gtccggccgg ctcgtgtcgg ccccacggca atagtcttcg gccaccaacc ctgaggagga
     1501 tcaatcaatg gacatgaagc gagcattcgg gctcgtgtgt gcgacggcgc ttgttgccgt
     1561 cgcgtgcggc ggcccggcag tcacgcagcg ggcatcgact ggcgcgagcc aggcgcccgc
     1621 gagcactgcc gcctcgagcg cgccgtcaac agcggcgagc accgagcctt ccgctgcagc
     1681 gagcgcccaa gccacgccgg gtgttgacct cggccactgc tcggacgcgg cggccgacct
     1741 ctcggcaacc tcaaccggcc cgaatggcga ggaatcgacg tcggcctccg atatcccgga
     1801 cctgaccgac gagcaggttg cggcagccaa tgcggccggc tacaagtggg catacctgcc
     1861 gtccggcgca tcgacctggt tcaacgccgt gggcgagggc gccaaggcag aggcagagcg
     1921 actgggtctt acgttgaccg tcacggccga ctcgaacttc gacccggcca agcaggccgc
     1981 ggacgtcgaa agcgccatgg caaccgaccc agacatcatc gtgactctgc ccgtcgaccc
     2041 gactcagggc gccgaggcct tccgtccggc ggttgatgcc ggcgtcaagc ttgtcttcaa
     2101 cgacaacggc gtcgacggtt tcactgccgg cgaggagtac gtcgcgatcg tcaccggcga
     2161 ccacttcggc atgggcgacg ctgcggccga tctgatgagc caggcgctgc ccgacggcgg
     2221 cccgatcggc gtcatcttcc acgacgcgga cttttacgtg accaacaacc gcgactgccg
     2281 cttcgtggca gctgtcgagc agaactaccc agagctcgag atcgtggcca cctccgggat
     2341 gaccgaggaa ggcgcgaccg aaagcatcac gtcggcgatg ctgacgcagc atcccgagat
     2401 caagggcatc tacgtcacat ggagcgcagc cgctacaggc gcgctggccg cactgcgcgg
     2461 tgccgatcgg tccgatgtca aggtcgtcgt ccacgacctg gatgcggcca acgatctgga
     2521 catggcccag aacggc
//
LOCUS       DATLCO010000089         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3825353, whole genome shotgun sequence.
ACCESSION   DATLCO010000089 DATLCO010000000
VERSION     DATLCO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3825353"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VMZ33_02235"
     CDS             <1..1833
                     /locus_tag="VMZ33_02235"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR16080.1"
                     /translation="PLEIRQRVLDRAEGNPLFVEEVVRMLIEEGVVERQGDRWVAKRE
                     AADIRVPENVEALIRARLDTLPTSERATVQAASVVGRIFQQSAVAAIAPSTEGSTLQQ
                     HLEDAILRDLITDERVHDEPTFRFRHILIRDVAYATLPKARRADLHRGVADWLRAWAG
                     ERIEEFAEIEAYHLEQSVRLRWELDGAIDPAERDRAVTALRSSAERAFARDDARATRT
                     FAERALALDLPSGDERLEIQALLAEALWRLSEYRKSGDVASKLEIEAATAGRKDLQGR
                     AILFKAGDIWLRLESADADVALRELERARPLLDEAGDRRALVFAHELMGWAGWWHGDL
                     ERSRVAFVQMRELAHELGWTSREAEAVMQLVGIAAHRGEVEECRALMDAARALAASGP
                     SRLTRARVDRAYGTNLGVLGQIGEGIQLLIDAGAILEEFGDRDEAFTANLHLGDFSRM
                     LGRPHEALAHYERAETQVLEHAGFLPEALRHKAQVLIGIGDLDRAVEHAERAVAITGR
                     DDWATVASTRMVLGMVREAQGRLGEAEDLLREAAEVMDRLDFNPWEEEIALAEFLLRR
                     GRPDEASEWLEKARASVVIFGPTSPFSADVEARAAAASAGAQRA"
     gene            2135..>2748
                     /locus_tag="VMZ33_02240"
     CDS             2135..>2748
                     /locus_tag="VMZ33_02240"
                     /inference="COORDINATES: protein motif:HMM:NF020060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16081.1"
                     /translation="MARNPAIKPVIPPVDDEGRFTGAGTKGLTFERRWTTPGIHPYDE
                     ILWELRSAEIKNESGGTVFEQTDIEVPNFWSQLATNVVVSKYFRGHIGTAGRERSVKQ
                     LIDRVVNTIAGWAETQHYFASPEDLSTFKAELTHLLVHQKMSFNSPVWFNVGVEERPQ
                     CSACFINSVRDEMGSIMDLAKTEAMLFKYGSGAGVNLSTIRGSK"
BASE COUNT          499 a          857 c          952 g          440 t
ORIGIN      
        1 ccgttggaaa tccgtcagcg ggtgctcgat cgggcggaag gcaatccgct gttcgtcgag
       61 gaagtcgtgc gcatgctcat cgaggagggc gtggtcgagc ggcagggtga tcgctgggtg
      121 gcgaagcgcg aggcggccga catccgcgtc ccggagaacg tcgaggcgct catccgcgca
      181 cgactcgaca cgctgcccac atcggagcgg gccacggtac aggccgcgtc ggtggtcggt
      241 cggatcttcc agcagtcggc ggtcgccgct atcgcgccgt cgaccgaggg atcgacactc
      301 cagcagcacc tcgaggacgc catcctgcgc gacctgatca ccgacgagcg agtgcacgac
      361 gagccgacct tccgattccg gcacatcctc atccgcgacg tcgcctacgc cactctgccc
      421 aaggcgcgcc gcgccgatct gcatcgcggc gtcgccgatt ggcttcgggc gtgggccggg
      481 gagcggattg aggagttcgc cgagatagag gcgtaccacc ttgagcagag cgtgcgcctg
      541 cggtgggagc tggatggggc gatcgatccc gccgaacggg accgggccgt gacggcgctt
      601 cggtcgtccg ccgagcgggc tttcgcgcgc gacgacgccc gggccacacg gacgttcgct
      661 gagcgcgcgc tggcgctgga cctgcccagc ggtgacgagc ggctggagat ccaggcgctg
      721 ctggctgagg cgctgtggcg gttgtcggag taccgcaaat cgggtgatgt ggcctcgaag
      781 ctcgaaatcg aagcggcgac tgcgggccgc aaggacctcc agggtcgggc gatcctgttc
      841 aaggctgggg atatctggct gcggctcgaa tcggccgacg cagacgtggc cctgcgcgag
      901 ctcgagcgcg cgcgcccgct tctcgacgag gccggcgatc gccgggcgct cgtgttcgcc
      961 cacgagctga tgggctgggc gggttggtgg cacggtgatc ttgagcgatc acgagtcgcc
     1021 tttgtgcaaa tgcgtgagct cgcccatgag ctgggatgga ccagccgcga ggccgaagca
     1081 gtcatgcaac tcgtcggcat cgcggctcat cggggcgagg tggaggagtg ccgagcgttg
     1141 atggatgcgg cgcgagccct cgctgcttcc gggccgagca gactcacgcg agcacgcgtc
     1201 gatcgggcat acggcaccaa tctgggcgtt ctgggtcaga tcggcgaagg cattcagttg
     1261 ctgatcgacg ccggtgcaat ccttgaagag ttcggcgacc gcgacgaggc ctttaccgca
     1321 aacctgcacc ttggcgactt cagccggatg ctcggtcggc cgcatgaggc actggcgcat
     1381 tacgagcgcg cggagacgca agtgctcgag catgccggct tcctgccgga ggccctgagg
     1441 cataaggcgc aggtactgat cgggatcggt gatctcgaca gagccgtgga gcacgccgaa
     1501 agggcagtgg ccatcaccgg tcgcgatgac tgggcgacgg tcgcatcgac ccgaatggtt
     1561 ctgggcatgg tccgagaggc gcagggtcgg ctaggggaag ccgaggacct gcttcgtgag
     1621 gcagccgaag tgatggatcg cctggatttc aacccgtggg aggaagagat cgcgctggcc
     1681 gagttcctcc tgcgccgcgg gcgaccggac gaggccagcg agtggcttga gaaggcgcga
     1741 gcgagcgtgg tcatattcgg tcctacgtcg ccgttcagcg ccgacgtcga ggcccgagcc
     1801 gcagcggcgt ccgcaggcgc tcagcgggcg tagtcctccg ggaaggccgt ccacgcacag
     1861 tccccagacg ctccacattg actccacgtg ccgtccacgc tcaaatgtga gtagccacta
     1921 cacctagcgt tacaaatgga ccatgaacgc tagatgttgt ggtttgagtc ttgacagcca
     1981 ctacccccgc gcctagcatt gaccgattgc gggcaccgcc caaatgcccc agagcgggcg
     2041 ccgcagagag gtagcgaggg accgggaaat ggctcgagca gcaagctcag cgacaaccag
     2101 ggaacagggt tccaccactg acggaaccaa ggttttggca cgtaatccag ccatcaagcc
     2161 ggtgatcccg cccgtcgacg acgagggtcg tttcacaggc gccggcacca agggcctgac
     2221 cttcgagcgt cgctggacaa cgccgggtat ccacccctac gacgagatcc tgtgggagct
     2281 ccgctcggct gagatcaaga acgagtcggg cggcaccgtc ttcgagcaga cggacatcga
     2341 agtccccaac ttctggagcc agctggccac caacgtggtc gtgtcgaagt acttccgcgg
     2401 tcatatcggg actgctggtc gtgagcgctc cgtgaagcag ctgatcgacc gtgtcgtcaa
     2461 cacgatcgcc ggttgggcgg agacccagca ttacttcgca agcccggagg acctgagcac
     2521 cttcaaggca gagctgaccc atctcctggt ccaccagaag atgagcttca actcgccggt
     2581 ctggttcaac gtgggcgtcg aagagcggcc gcagtgcagc gcctgcttca tcaactccgt
     2641 ccgcgacgag atgggcagca tcatggacct cgccaagaca gaggccatgc tgttcaagta
     2701 cggctcgggc gcgggcgtca acctcagcac catccgtggc tccaagga
//
LOCUS       DATLCO010000090         3195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9035557, whole genome shotgun sequence.
ACCESSION   DATLCO010000090 DATLCO010000000
VERSION     DATLCO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9035557"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..952
                     /locus_tag="VMZ33_02245"
     CDS             119..952
                     /locus_tag="VMZ33_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HUR16082.1"
                     /translation="MSRMPGWGMAMGMVHTMVRFFQPKVTVQYPDVVQEISVHHRGRL
                     ILLYDEAGNLKCETCFQCAMACPVEIIDMGGVDTKNRYSLHWGPAEQYGERREESPMR
                     RSGRTVPDRTFAAWLPIDLGPLDRILVKHEYDPRRLLSILEDTQAQYGYLPVAAIQHI
                     SHATGAWYSEIYGIATSYANLIFEPGRATEIGICRCPACTMRGGGRIRDALVGALGTD
                     VGGVTVDGSVRFVAAECGGEASAQPLVTVDSQPQPGITADKARSLAESLRAKSTRPAQ
                     A"
     gene            965..2287
                     /locus_tag="VMZ33_02250"
     CDS             965..2287
                     /locus_tag="VMZ33_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HUR16083.1"
                     /translation="MNILRGPKGVASVLLARIDRLSTDSPLPAAESAGAWSAWKSTVP
                     QTSPQALVRQMTDSGLRGRGGAGYLTGDKWCAAASVDADVRYVVANGFEADPGAQVDR
                     LLMERDPHAIVEGLALAAYAVGATRAYIAVRANMATALRAFTTAVRAAEEAGYLGTGV
                     LGTGYDLHVEVVAIPGGMVVGEETTLIKAIENKRAQPDQRPPFPTGRGLFGRPTVVNN
                     VETLALVPWIVTNGAAAFAAIGDDTNPGTTLIQISGAVANPGVVEAPLGMSLRKILDL
                     AGGAANAKALLVGGPAGGFLPATELDTLYSPTELAAKGAIWGSGSITVVDESTCIVDM
                     ATLLLRYLSDESCGKTIPCRIGVRRLYELGQRATSGLSRPNDPAVAQQLAADVRDGAL
                     CGLERLAVNPFTSGMRYFAAEFDQHFTRGTCPAGVCSPVRVVAQPMSA"
     gene            2284..>3195
                     /locus_tag="VMZ33_02255"
     CDS             2284..>3195
                     /locus_tag="VMZ33_02255"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR16084.1"
                     /translation="MTDTLVPQTDSRLPSVLDPAAPGVGSEAPDAFVERLLETQAPQR
                     RMSSASFTVEVDGKQYTGRDGQTILEICRDNGIDVPTLCYEPKLPGFGACRMCVVEVE
                     GEEHPPISCSRAAEPDMVVQTQTQRLREIRKTNLELIFSDHNAYCLPPCQNKCPSHID
                     IPGFLKANTEGNFMESARIFKRTIPFPSVLGRVCPAPCEEHCRRDEVEEAIAIRDSHR
                     YAGDQVLKAQSGGVKAPVPFEVQPKTGKRVAVIGSGPTGMSTAYYLLIAGHDVTVFER
                     DPAPGGMLRYGIPQYRLPKVEVLEGEYD"
BASE COUNT          556 a         1093 c         1031 g          515 t
ORIGIN      
        1 atgggcttcg cgtggaagtg gatgattgag atcgcgatcc tgaacatctt cgtgacggcc
       61 ggggcaatcc ttctcgtcga ggagctccag agaaggtgag cgcgcaagca tgggtttgat
      121 gtcgagaatg ccgggctggg gcatggccat gggcatggtc cacaccatgg ttcggttctt
      181 ccaaccgaag gtcacggtcc agtacccgga cgtcgtccag gaaatctccg ttcaccaccg
      241 cggacggctg atcctgctct acgacgaagc gggcaacctc aagtgcgaga cgtgcttcca
      301 gtgcgcgatg gcgtgtccgg tggagatcat cgacatgggc ggcgtcgaca ccaagaaccg
      361 ctacagcctg cattggggcc ccgcagagca gtacggcgag cggcgcgagg agtcgccaat
      421 gcgccgcagc ggccggaccg tgccagatcg gacgtttgcc gcgtggctgc caattgacct
      481 cggtccgctc gatcgaatcc tggtcaaaca cgagtacgac ccgcggcgcc tgctgtcgat
      541 cctcgaggac actcaggccc agtacgggta cctgcccgtg gctgccatac agcacatcag
      601 ccacgccacc ggggcgtggt acagcgagat ctacggcatc gcgacgtcat acgcgaacct
      661 catctttgag cccggccgag ccacggagat cggcatctgc cgctgtccgg catgcaccat
      721 gcgcggcggc gggcggattc gagatgcgct cgtcggtgcg ctagggacgg acgtcggcgg
      781 cgtcaccgtc gatggttcgg tgcgcttcgt ggcggcggag tgcggcggcg aggcgtcggc
      841 gcagccactc gtcacagttg acagtcagcc ccagccgggc atcaccgccg acaaggcacg
      901 ctccctggct gagtcgctcc gcgccaagtc cactcgtccg gcgcaggcgt agctcgcagc
      961 cgcgatgaac atcctcaggg gcccgaaagg cgtcgccagc gtgctgctgg cacgcatcga
     1021 caggctaagt accgattcgc cacttccagc tgccgagtcg gcgggtgcct ggtcggcgtg
     1081 gaaaagcact gtcccacaaa cgagccccca ggctctcgtt cgacagatga ccgactcagg
     1141 cctgcgcggg cgtggcggcg ccggctacct caccggtgac aaatggtgcg cggctgccag
     1201 tgtcgacgca gatgtgcggt acgtggtcgc caatggcttc gaggcggacc ccggcgcaca
     1261 ggtcgatcgc ctgctcatgg agcgcgaccc gcacgccata gtcgagggcc tggccctggc
     1321 ggcctacgcc gtcggcgcga cgcgcgccta catcgctgtc cgagccaaca tggccacggc
     1381 tttgcgcgcg ttcacaactg ccgtccgcgc cgccgaggaa gccggctacc tcggcaccgg
     1441 cgtgctggga accggttacg accttcatgt cgaggtcgtg gccatcccgg gtgggatggt
     1501 cgtcggcgaa gaaacgacgc tcatcaaggc gattgagaac aaacgtgccc agccagatca
     1561 gcggcccccc ttccccacgg ggcgcggcct ctttgggcgg ccaacggttg tcaataacgt
     1621 cgagacgctt gccctcgtgc cgtggatcgt caccaacggc gcggcggctt ttgccgcgat
     1681 cggtgacgac acgaacccgg gcacaacgct catccagatc agcggggcgg ttgcgaaccc
     1741 gggtgtcgtc gaggcgcccc tggggatgag cctgcgcaag atcctcgacc ttgccggcgg
     1801 cgccgccaac gccaaggcac tgctcgtcgg cggcccggct ggcgggtttc tgccggcaac
     1861 agagctcgac acgctctact caccgaccga gcttgcggcc aagggcgcca tctggggatc
     1921 cggcagcatc accgtcgtgg acgaatcgac gtgcatcgtc gacatggcca ctctgctgct
     1981 gcgctacctc tccgacgagt catgcggcaa gaccatcccg tgccgcatcg gcgtccgtcg
     2041 cctatacgag ctgggccagc gcgccacgag cggcttgtcc aggcccaacg atccggcggt
     2101 cgcccagcag ctcgctgccg acgtccgcga cggcgccctg tgtggcctcg agcggctcgc
     2161 ggtcaatccc ttcaccagcg gaatgcgata cttcgccgct gagttcgatc agcacttcac
     2221 gcgcggcacg tgccctgcgg gcgtttgcag cccggtccgt gtcgtagccc agcccatgag
     2281 cgcatgaccg acacactcgt tccccagaca gatagccgcc tgccttcggt gctcgatccc
     2341 gccgcgcccg gcgtcgggtc agaggcaccc gacgcgttcg tcgagcgcct gctcgagaca
     2401 caggcccccc agcgtcgcat gagctcagcc agcttcacgg ttgaagtcga cggcaagcaa
     2461 tacacgggcc gcgacggcca gaccatcctc gaaatctgcc gcgacaacgg cattgatgtc
     2521 ccaacgctct gctacgagcc caagctgccc ggctttgggg cgtgccgcat gtgcgtcgtt
     2581 gaggttgaag gcgaggaaca tccgcccatc tcctgttcgc gcgccgcgga gccggacatg
     2641 gtggtgcaaa cgcagaccca acgcctgcgc gagatccgca agaccaacct cgagctgatc
     2701 ttctcggacc acaacgccta ctgcctgccg ccgtgccaga acaagtgccc gagccacatc
     2761 gacatcccgg gcttcctgaa ggccaacacc gaaggcaact tcatggaatc ggcgcggatc
     2821 ttcaagcgca cgattccgtt tccgagcgtt ctcggccgag tctgcccagc gccgtgcgag
     2881 gagcattgcc gtcgcgacga ggtcgaggag gcgattgcga tccgcgattc gcaccgctac
     2941 gccggcgacc aggttctgaa ggcgcagtcg ggtggcgtca aggcgcccgt cccctttgag
     3001 gtgcaaccga agacgggtaa gcgcgtcgcg gtcatcggct ccgggcccac cggcatgtcg
     3061 accgcctact acctgctgat cgccggccat gacgtcaccg tcttcgagcg tgacccggcg
     3121 ccgggcggaa tgctgcgcta cggcatcccg caatatcgcc tgcccaaggt ggaggtcctc
     3181 gaaggcgagt acgac
//
LOCUS       DATLCO010000091         7036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5316986, whole genome shotgun sequence.
ACCESSION   DATLCO010000091 DATLCO010000000
VERSION     DATLCO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5316986"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VMZ33_02260"
     CDS             <1..883
                     /locus_tag="VMZ33_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HUR16085.1"
                     /translation="RVDESSPMVDARLVDGSRVNVIIPPISLVGPVLTIRKFASTPMT
                     TDDLIRFGTATPEMFDFLRACVEARLNLFVSGGTGSGKTTMLNVLSSFIPNDERIVTV
                     EDAAELQLRQEHVVTLESRPANIEGKGAVPIRELVRNALRMRPDRIIVGECRGAEALD
                     MLQAMNTGHDGSMSTGHANTPRDMLSRLETMVLFAGVELPLRAVREQISSAVDLIVHQ
                     DRLKDGTRKITNITEVQGMEGDVIVLQDVFVFEQTGISEGKVQGHLKPTGVRPRFSEK
                     FEAQGIHLPPRLFGSPF"
     gene            887..1897
                     /locus_tag="VMZ33_02265"
     CDS             887..1897
                     /locus_tag="VMZ33_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUR16086.1"
                     /translation="MEPLTILIAALAAVGVLLIFVSLAGGSDVNARLERYGAPNADEK
                     AASAGQPRRDLGERIGSSAALVGFNRVVERRDWGSNLARELARADLMLKPSEFLAIRV
                     GAMLGVPLVMIILSPLIGFFGSPLLWVLGVVIGYWVPRFWLNRRKAKRLKAFNSGLAD
                     TITLLANSLRAGSSFLQSVEMVVREAQPPISTEFARVIREVNLGLPLEEALANLQRRV
                     KSEDLDLMTTAISIQHSVGGNLAEILDTIAFTIRERVRIKGEIKTLTAQQRMSGYVVG
                     FLPVALVLIMSVIAPTFMEPMFRKPPELLGLPAGLFILGLGGIMMLIGFVLIRRIVDI
                     EV"
     gene            1901..2830
                     /locus_tag="VMZ33_02270"
     CDS             1901..2830
                     /locus_tag="VMZ33_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUR16087.1"
                     /translation="MPLIPLLMAALAAIGVLIVFYAIAGARPMDPVQARLSQLGSMQA
                     KTLEELELQQPLFERTLRPLATRLSGLAQRFASPQKIGRTEKRLAMAGNPGDLRTIDF
                     LGLKLVVATLWALGFFVFSLLMLGNPGFGFVGAGALGALGFFLPEIWLSRRIKGRQKA
                     ILMAVPDTLDLLTISVRAGLSFDGALSKVIEKVRGPLSVEFRRCLAEIRVGRARRDAL
                     RDIVVRTDVPALTNFIGAIVQAEQLGVPIAKVLQIQSDQLRIERRQRAEELAAKAPIK
                     MLFPLVGCIFPSMFIVILGPAVILIALNMSNGA"
     gene            2853..3239
                     /locus_tag="VMZ33_02275"
     CDS             2853..3239
                     /locus_tag="VMZ33_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HUR16088.1"
                     /translation="MERLVLTNGAPGQRLFTLEVAESFTQKLRGLIGRRSLDPGEGLY
                     FPGTNSLHMLFMRFAIDAVFLGPPGPDGRSEIVAVRDRLRPWLGVVWWVRGVRGRGGV
                     IELAAGAASAAGLRKGDLVRMERLAP"
     gene            complement(3247..4407)
                     /locus_tag="VMZ33_02280"
     CDS             complement(3247..4407)
                     /locus_tag="VMZ33_02280"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HUR16089.1"
                     /translation="MATAIAAISLVLILGMMMTWMGPSAGWAYDFQAYHDAALRLLET
                     GSPYQEQTLSGPFRPGPGGLYLYSPILALVLAPLTNLQFDAAALTWLALRLVALAATC
                     ALMPVRSTIRIATFCAALISAPVLFDLNLGNVSLLVTLAAVVMWRYLDKPAAAIALAA
                     SLTLRPTMAVIAAWWLMRGLWRPVAWALGAGLAMLLLSLTFVAPGVWGEYLTVLRNVS
                     DVTGVRSNVDLGSAVLMMEGPTWLATIALFVGYAVAIGASLFSLRRDRELSFVVTLMA
                     TLLMAPLLWEHYLTNLLIPAAFLAGRGRTWGLILPLLGWTPTLIAMLIPSLRGSAEAV
                     LPLIALLGVLAPFLAPSRGEPAGWFANRLGGHSEGVDEKLVKEPAPTLATAD"
     gene            4587..5243
                     /locus_tag="VMZ33_02285"
     CDS             4587..5243
                     /locus_tag="VMZ33_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HUR16090.1"
                     /translation="MLKVALDLLLPPKCPGCGTEGEAICAGCRTLMQRRLDEPAGVPI
                     GLPTRQPAGLVQLEWCCPMNGPARAAIHALKYDGERRLVDQLSKLMAARWRVAGIGGE
                     LLVPVPVHAARRRERGFDQAELLARQLGRELHLPTAYALQRAAKTAAMHKLGRDAREA
                     NVRSAFAVAPRHAAAVNQRWIVLVDDVVTTGATLSGCARALYEAGASAVSALTLARER
                     "
     gene            complement(5189..5857)
                     /locus_tag="VMZ33_02290"
     CDS             complement(5189..5857)
                     /locus_tag="VMZ33_02290"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUR16091.1"
                     /translation="MDPKLLTALKALSDASRLRIVGLLAEGKRMSVEQLAAALGLTAG
                     TVVHHLKRLREAGLVDSTPRPPYMDYWLKFERLAEIGGSLHRLAREQAGASTETFIRP
                     AWATAEEGRVLHAFLDGDRLLSIPAQHAKRLVVLRYLAETVFERKRKYPEKEVNQLLA
                     VRNPDAASLRRYLVDENFMSRKNSVYRLRPRKDWPERADAVESHDQRSRASVSADTAE
                     APAS"
     gene            5924..6961
                     /locus_tag="VMZ33_02295"
     CDS             5924..6961
                     /locus_tag="VMZ33_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HUR16092.1"
                     /translation="MTESNTAAAPERVIGPHLALGTGLLKAADRAREIGATAVQIFTD
                     NPTAWRRRREPPAKLDAFRERLAQGGIVSLAVHAPYLINLCGGNEDFWHKSIASVANE
                     LEVGSTYGAAFVVMHIGSHRGLGREEGIRRLVAGMSESLKAADELHTQAATEGGNESE
                     AASYGRSGRPPLPKLVLENAPGSGDAIGASMEDLADIFEASAAAGLDMARLGVCLDTA
                     HLWAAGYDLATSGGAEALLHRIDGILGRERVVMLHLNDSKTVVGSRVDRHEHIGAGQM
                     GPRGIGQVVGHPWLANLPTFLETPGMDTGYDAVNLRRVRLILDGQELPDLPPEAFTLR
                     SSRSRSGPPQV"
BASE COUNT         1193 a         2361 c         2314 g         1168 t
ORIGIN      
        1 acgggtggac gaatcttcgc cgatggtcga cgcccgcctt gtggacgggt cgcgcgtcaa
       61 cgtcatcatc ccgccgatct cactggtcgg cccggtgctc accatccgca agttcgcctc
      121 cactccgatg acgaccgacg atctgattcg cttcggcacc gcgacacctg agatgttcga
      181 cttcctgcgg gcatgcgttg aggcgcgact gaacctcttt gtctcgggcg gtacgggttc
      241 cggcaagacc acgatgctca acgtcctgtc atcgttcatc cccaacgacg agcgcatcgt
      301 gacggtggag gacgcggccg agctccagct ccgccaggag cacgtcgtga cgctcgagtc
      361 ccgcccagcg aacatcgagg gcaagggcgc cgtcccaatc cgcgagctcg tgcggaacgc
      421 actccgaatg cgccccgatc gcatcatcgt cggcgagtgc cgcggcgcgg aggcgctgga
      481 catgctccag gcgatgaaca cgggccacga cggctccatg tcgacgggcc acgcgaacac
      541 gccgcgcgac atgctctccc gcttggagac gatggtcctg tttgcgggtg tcgagctgcc
      601 gttgcgtgcc gtgcgcgagc agatcagctc ggcggtcgac ctcatcgtcc accaggaccg
      661 tctcaaggac gggacccgca agatcaccaa catcactgaa gtccagggca tggaaggcga
      721 cgtgatcgtg ctccaggacg tgttcgtctt cgagcagacg ggaatcagcg agggcaaggt
      781 ccagggccac ttgaagccta ccggcgttcg cccgcgcttc tccgagaagt tcgaggcgca
      841 gggcatccac ctgccaccgc gactgttcgg aagccccttc taggccatgg aaccgctcac
      901 gatcctcatc gcagccctgg ccgcagtcgg tgtgctgctg atcttcgtaa gcctggccgg
      961 cgggtcagac gtaaacgcgc ggctcgagcg ctacggagcg ccgaacgccg acgagaaggc
     1021 cgccagtgcg ggccagccgc gccgcgacct cggcgagcgc atcggctcgt ctgcggcgtt
     1081 ggtcggcttt aaccgcgtcg tcgagaggcg cgactggggc agcaacctcg cccgcgaact
     1141 ggcccgcgcc gacctcatgt tgaagccgag cgaattcctg gccatccgcg tcggcgcgat
     1201 gctcggcgtg ccgctggtaa tgatcatcct cagcccgctc atcggcttct tcgggagccc
     1261 gctcctgtgg gtgctcgggg tagtcatcgg ctactgggtc ccgcgcttct ggctgaatag
     1321 acgcaaggcc aagcggctca aggccttcaa ctccgggctg gccgacacca tcacgttgct
     1381 cgccaactcg ctacgtgcag gctcctcctt cctccagtcg gtggagatgg tcgtccgcga
     1441 ggcacagccg ccgatctcga cagagttcgc gcgtgtcatc cgcgaggtga acttgggctt
     1501 gccccttgaa gaggcactcg ccaacctcca gcgccgcgtc aagtcagagg acctcgacct
     1561 gatgaccacg gcaatctcaa tccagcactc cgtcggtggc aacctggccg agattctcga
     1621 caccatcgcc ttcacgatca gagagcgcgt gcgcatcaaa ggcgagataa aaacgctcac
     1681 cgcgcagcag cgcatgtctg gttacgtggt cggattcctg ccggtcgccc tggtactgat
     1741 catgtcggtc atcgcaccca ccttcatgga gccgatgttc cgcaagccgc ctgaactgct
     1801 tggactgccg gctggcctgt tcattctggg actgggcgga atcatgatgc tcatcggctt
     1861 cgtgttgatc cgccgcatcg tcgacatcga ggtctaggag atgccactca tcccgcttct
     1921 gatggcagct ctcgccgcta tcggcgtcct gatcgtcttt tatgcaatcg ccggcgcacg
     1981 gccgatggac ccggtccagg cccgactctc ccagctgggc tccatgcagg ccaagacgct
     2041 cgaggagctc gagctccagc agcccctctt cgagcgcacg ctgcgacctc tggcgacgcg
     2101 gctctcgggc ctcgcgcagc gcttcgccag cccgcagaag atcggccgca ccgagaagcg
     2161 gctggcgatg gccggcaacc cgggcgacct gcgcaccatc gacttcctcg ggttgaagct
     2221 cgtcgtcgcg accctgtggg cgcttggctt cttcgtattc agtctgctca tgctcggcaa
     2281 ccccggattc ggcttcgtcg gtgccggcgc gctcggtgcg ctcggcttct tcctccccga
     2341 gatctggttg agccggcgca tcaaggggcg ccagaaggcc atcctgatgg ccgtgccgga
     2401 cacccttgac ctactgacca tttccgtccg tgccggcctg tcgttcgacg gggcgctgtc
     2461 caaggtcatc gagaaggtcc gcggtccact gtcggtggag tttcgccggt gcctcgcgga
     2521 gatccgcgtc gggcgggcca ggcgcgacgc tttgcgcgac atcgtcgttc ggacggatgt
     2581 accggccctg accaacttca tcggggccat cgtccaagcc gagcagctcg gcgtaccgat
     2641 cgccaaggtg ctgcagatcc agtcggatca gctccgcatc gagcgtcgcc agcgggccga
     2701 ggagctcgcc gcgaaggcgc ccatcaagat gctcttcccc cttgtgggct gcatcttccc
     2761 gtcgatgttc atcgtcatcc ttggcccagc ggtcatcctg atcgcgctca acatgtccaa
     2821 cggggcctga gcgccgccac tagccttccc tcgtggagcg gctcgtcctg accaatggcg
     2881 ccccgggcca gcgcctcttc acgcttgaag ttgcagagtc gttcactcag aagctccgtg
     2941 gcctgatcgg ccgtcgttcg ctcgatccgg gcgagggcct gtacttcccc ggcaccaact
     3001 cgctgcacat gctgttcatg cgtttcgcca tcgatgccgt gttcctgggc ccgccgggcc
     3061 ccgatggccg cagcgaaatc gtggccgtac gcgatcgcct gcgcccgtgg ctcggcgtcg
     3121 tgtggtgggt ccgcggcgtc agggggcgtg gcggtgtcat cgagctggcg gcgggcgcgg
     3181 cgtccgcggc agggctgcgc aaaggcgatc tggtgcggat ggagcggctc gcgccctagc
     3241 cgttcctcag tcggcggtgg ccagcgtcgg cgccggctcc ttgacgagct tctcatccac
     3301 gccctcgctg tggccgccga gacgattcgc gaaccagccg gccggctcgc cgcgcgacgg
     3361 cgcaaggaag ggcgccaaca cgccgagcag cgcgatgaga ggcagcaccg cctcggccga
     3421 tccacggaga gacgggatca acatcgcgat cagtgtcggc gtccagccga gcagcggcag
     3481 gatgaggccc caggttcggc cccgacccgc caggaaggcc gcggggatga gcaggttcgt
     3541 gaggtagtgc tcccatagca ggggagccat cagcagggtc gccatgagcg tcaccacgaa
     3601 gctcagctcg cggtcacggc ggagactgaa gagggaagct ccgatcgcca cggcgtagcc
     3661 gacaaacaag gcgatcgtgg ccagccacgt cgggccctcc atcatgagga ccgcagaccc
     3721 gaggtcgacg ttgctgcgga cgcccgtgac atcgcttacg ttgcgaagca cggtcaggta
     3781 ctccccccag acgccgggcg ccacgaacgt cagtgagagc aggagcatcg cgagcccggc
     3841 accgagagcc cacgccaccg ggcgccacaa cccgcgcatc aaccaccacg cggcgatcac
     3901 ggccatcgtt ggtcgaaggg tcaatgacgc ggcgagggcg atggcagccg ccggtttgtc
     3961 gaggtagcgc cacatgacga ccgctgcaag cgtgaccagc aggctgacat tgcccagatt
     4021 gaggtcaaac aggaccggcg cgctgatgag ggccgcgcag aaggtggcta tccggatcgt
     4081 cgatcggacg ggcatcagag cgcacgtggc cgcgagtgcg acgaggcgca acgccagcca
     4141 ggtcagggcg gcggcgtcaa attgcagatt cgtaagtggc gccaggacca gagcgaggat
     4201 cggcgagtag aggtacaaac cgcccggccc gggtcggaaa ggcccgctga gcgtctgctc
     4261 ctggtacggc gacccggtct cgagtagtcg cagggccgca tcgtggtagg cctgaaagtc
     4321 gtaggcccaa ccggctgagg gacccatcca ggtcatcatc atccccagga tgagcaccag
     4381 gctgattgcc gcgatcgctg tcgccaaggg cgcgcgccaa cgcgcgctga gtgaggacct
     4441 gggcgtcgac cgactggaca cggctattga attatcaagc cctcggagcc acgagaaagc
     4501 gagcgcttcc acccggatcc caattgggtg ataaccaggc gataaccggc ggattagccg
     4561 ccatcgccag tctgtgacat gacgtcatgc tgaaggttgc cctggatctc ctgttgcccc
     4621 ccaaatgtcc cggctgcgga accgagggtg aggctatctg cgccggttgc cggacgctca
     4681 tgcagaggcg cctcgacgag cccgccggcg taccgatcgg cctgccaacg cgccagccgg
     4741 caggactcgt ccaactggag tggtgttgcc caatgaacgg cccggcgcgg gccgcgatac
     4801 acgccctgaa gtacgacggc gaacggcgtt tggtcgacca actcagcaag ctgatggcag
     4861 ctcgctggcg tgtagccggt attggcggcg agctgctggt cccggtgccc gtccacgccg
     4921 cgcgccgacg cgagcgcggt tttgaccagg ccgagctgtt ggcgcgccag cttggcaggg
     4981 aactgcacct cccgaccgcc tacgcgctcc agcgcgccgc gaagacggca gcgatgcaca
     5041 agctcggtcg cgacgcgcgc gaggccaacg tgcggagcgc gtttgccgtg gcaccgcgtc
     5101 acgcggcagc ggtgaaccag cgctggatcg tcctcgtaga cgacgtggta accacgggcg
     5161 caacactgtc gggctgcgcc cgcgctctct acgaggccgg cgcctccgcg gtatcagcgc
     5221 tgacgcttgc gcgagagcgc tgatcgtgcg actcgacagc atcggcgcgc tcgggccagt
     5281 ccttgcgcgg tcgcaggcgg taaacgctgt tcttgcgcga catgaagttc tcatcgacga
     5341 ggtaacgccg gagcgaagcc gcgtccgggt tacgcacggc caagagctgg ttgacctctt
     5401 tttcggggta cttgcgcttg cgctcaaaca cggtctcggc gaggtaccgc agcaccacca
     5461 gccgtttggc gtgttgtgcc ggaatgctga gcagccggtc gccgtcgagg aacgcatgca
     5521 acacgcggcc ctcctccgcc gtggcccaag ccggcctgat gaaggtttcg gtcgatgcgc
     5581 cggcctgttc gcgcgccagt ctgtgcagtg atccgccgat ctcagccagg cgctcgaact
     5641 tgagccagta atccatgtac ggcgggcgcg gggtcgagtc gaccaggccc gcctcccgga
     5701 gtcgcttcag gtgatggacg accgtgccgg ccgtcagccc cagggcggcg gccagctgct
     5761 cgaccgacat gcgcttgccc tccgcgagca atccgacgat ccgcaggcgc gacgcgtcag
     5821 agagcgcttt gagggctgtc aacagcttgg ggtccatgtc gccagatggt aacgcggttt
     5881 gataaacatc aaaggggcat aacgcgtcta gcatccgaag ctgatgacgg agagcaacac
     5941 cgcggcggcg ccagagcggg tgattggacc acacctcgcc cttggtacgg ggctgctgaa
     6001 agccgccgac cgggcgcgcg agatcggcgc gacggcggtg cagatcttca cggacaatcc
     6061 gaccgcgtgg cgtcgtcgac gggagccgcc ggcgaagctc gacgccttcc gcgaacgact
     6121 cgcccagggc ggaatcgtgt cccttgcggt ccacgcgccg tacctgatca acctgtgtgg
     6181 tggcaacgag gatttttggc acaaatccat tgcttcggtc gccaacgagc tcgaggttgg
     6241 ctccacctat ggcgccgcgt tcgtcgtgat gcacatcgga tcgcatcgcg gcctgggtcg
     6301 cgaggagggg atccgtcggt tggtcgcggg catgagcgag tcgctcaagg cggccgacga
     6361 gctgcacacc caggcagcca cggagggcgg caatgaatcg gaagccgcct cgtacggccg
     6421 cagcggacgc ccgccgctgc cgaaactggt cctcgagaac gcgcccggct caggagacgc
     6481 gatcggcgct tcgatggagg acctggccga catctttgag gcctcggcgg cagccggcct
     6541 cgacatggct cgcctcggcg tgtgccttga cacggcacac ctgtgggccg cgggctacga
     6601 ccttgctacc agcggtggcg cagaggcgct gctacatagg atcgacggca tcctcggccg
     6661 ggagcgcgtg gtgatgctcc atctgaacga ctccaagacc gtcgtcggct ccagggttga
     6721 tcgccacgag cacatcggag ccggacagat ggggccgcgc ggcatcggcc aggtcgtcgg
     6781 tcatccgtgg ttggcaaatc tgccgacgtt tctcgagacg ccgggcatgg ataccggtta
     6841 cgacgccgtg aacctccggc gcgttcgtct catcctcgac ggccaggaac ttcctgatct
     6901 cccgccggaa gcgttcacgc tgcgcagctc gaggtcgcgc agcggtccgc cccaggtcta
     6961 acccggtcgt gcccgaagcg accgtaccaa gggcctatat cgaccagcgg gggcgcaccg
     7021 gaacatgccc ttgtcg
//
LOCUS       DATLCO010000092         2531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3826184, whole genome shotgun sequence.
ACCESSION   DATLCO010000092 DATLCO010000000
VERSION     DATLCO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3826184"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..893
                     /locus_tag="VMZ33_02300"
     CDS             114..893
                     /locus_tag="VMZ33_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUR16093.1"
                     /translation="MTGARAGDWLRSYLPALVVFVAVITVWELSTAGPGRRVLPAPSA
                     IVGAALENQATLIRSTRATFFEAIGGLGIGTLGGLAVAFAAARWSVARDVALPIAIAS
                     STIPIIAMAPILNNWFGVLNPLSKMMMAALLVFFPVMINVTRGLTEVKPASLELMRSY
                     AATDVAVLRKVRIPNMLPFFFTALKVGTTLSFIGAVVGEYFGGTSEVLGQFVLTSMSS
                     GAFDRAWAGILIGASAAIMAYLVVSIIERVAIPWHSSLATE"
     gene            1055..2149
                     /locus_tag="VMZ33_02305"
     CDS             1055..2149
                     /locus_tag="VMZ33_02305"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR16094.1"
                     /translation="MRDLRKSLALLVGAVLVAACQSAAPTQAPLTPVELQLQWEPQAQ
                     FAGYFAADKEGYYAAEGLDVEILRGGAEIIPQVVGSDPNGPEFTISWVPKVLEVRANG
                     QSDLVDIAQIFQRSGTRSVSWVDSNIESPADFAGKNVGVWAFGNEYEVTAAGLKEGLV
                     ENEDYTKVIQPFNMDLLLNREIDVAEAMIYNEYAQVLEAENPDTGELYQPSDLNVIDY
                     NAVGTAMLQDAIFARQAWLAQAGNEEIAVKFLRASFKGWMFCRDNPAKCIEYTVAAGS
                     TLPAGHQRWMMNEINPLIWPSPNGIGQLPMDTYNQTISIAKDALIIPGDPDPAAVRTD
                     LAERARVGITGDMNGANFQKGTVEVTAGGN"
BASE COUNT          432 a          847 c          795 g          457 t
ORIGIN      
        1 acgcgcgaga ctgaccgcta ctttgagctt gtgacggagg tccgcgagag cctgcgcggc
       61 cgacttggag gcgcggcctc agcgcagcgc acggcagccg agggcgctgt cgggtgaccg
      121 gcgcccgcgc cggcgactgg ctccgcagct acctgccggc gctcgtcgta ttcgtggccg
      181 taatcacggt gtgggagcta tccacggcgg gcccaggccg ccgcgtcctg cccgcgccca
      241 gcgccatcgt cggggcggcc ctcgaaaatc aagcgacgct catccgctcc acccgtgcga
      301 cgttcttcga ggccatcggc ggtctcggaa tcgggacgct cggcggcttg gctgtggcgt
      361 tcgcagcggc acgctggtcg gttgcccggg acgtcgcact cccgatcgcc attgcctcga
      421 gcaccatccc gatcatcgcc atggcgccga tcctcaacaa ctggttcggc gtcctcaacc
      481 cgttgagcaa gatgatgatg gcggctctcc tggtcttctt cccggtgatg atcaacgtca
      541 cccgcggtct gaccgaggtc aagccagcct cgctggaatt gatgcgttcc tatgccgcga
      601 ccgacgtcgc cgtgctgcgc aaggtacgaa tcccgaacat gctgcccttc tttttcactg
      661 cgctgaaggt gggcacgacg ctcagcttca tcggcgccgt tgtcggcgag tacttcggcg
      721 gaacgagcga agtcctgggc cagtttgtgc tgacgagcat gagcagcggc gcgtttgacc
      781 gagcgtgggc ggggatcctc atcggcgcct ccgccgcgat catggcgtac ctcgtcgtgt
      841 cgataatcga acgtgtagcc atcccctggc attcctcgct ggccaccgaa taggcctgcc
      901 cgaaccgact gatcgctctg acatggtgga aaactcgctc gcgccagccc cgacttgcgc
      961 tatcgtgccg ccgggattgg gcgagcgaca gccagggagg tgtcgtcgcc ggatggcgac
     1021 cgccaccatc tgcggtcaag aggaggatct gtctatgcgc gacctgagaa agtcgcttgc
     1081 gctcttggtg ggcgccgtgc tcgtagccgc gtgtcagtcg gccgcgccga cccaggcacc
     1141 gctaaccccc gttgagctac agctgcagtg ggagccacag gcccagttcg ccggctactt
     1201 cgccgcggac aaggagggct attacgcagc cgagggcctc gacgtcgaga tcctgcgcgg
     1261 cggcgcggag atcattcctc aggtggtcgg ctcggatccg aacggccctg agttcacgat
     1321 ctcgtgggtg cccaaggtgc tcgaggttcg agccaatggc caatccgacc tggtcgacat
     1381 cgcccagatc ttccagcgct cgggtacgcg ctcggtttcg tgggtcgact cgaacatcga
     1441 atcgcccgcc gacttcgctg gcaagaacgt cggtgtctgg gctttcggca acgagtacga
     1501 agtcaccgct gccggcctga aggaaggcct cgtggagaac gaggactaca ccaaggtcat
     1561 ccagccgttc aacatggacc tgctgctcaa ccgtgagatc gacgtcgctg aggcgatgat
     1621 ctacaacgag tacgcacagg tactcgaggc ggaaaacccc gatacgggcg agctctacca
     1681 gcccagcgat ctgaacgtga tcgactacaa cgcggtcggc acggccatgc tccaggatgc
     1741 gatcttcgcg cgccaggcat ggctggccca ggccggcaac gaggagatcg cagtcaagtt
     1801 cctgcgggcg tcgttcaagg gctggatgtt ctgccgcgac aaccccgcga agtgcattga
     1861 gtacaccgtc gccgctggtt cgacccttcc ggccggccat cagcgctgga tgatgaacga
     1921 gatcaatcct ttgatctggc cctcgcccaa cggcatcggc cagttgccaa tggacacgta
     1981 caaccagacg atttcgatcg ccaaggatgc cttgatcatc ccgggtgacc cggatccagc
     2041 ggccgtccgc accgatctcg ccgagcgcgc ccgtgtgggc atcaccggcg acatgaatgg
     2101 cgccaacttc cagaagggca ccgtggaggt cacggcaggg ggcaactagc cctgaaatcc
     2161 caaccgctcc cggtaattcg gaggccggcc cgcaagggtc ggcctccttt cttttgcctt
     2221 cctcaacgga tatggtgcag gcggaggtac gaccgtgaat ctcacacgat cgacgacact
     2281 cgcagtcacg ctcggcgact acgtctggac cgattggtgc gccggcgccg ctgactgact
     2341 cgcctgatcg acgtctccca caacccgccg cccagcggcc ctagtcggca gacgtgcccg
     2401 acccactgat cctggctgcg cttttcttcg cggcggccat tctctattca tccgtaggtc
     2461 acgccggtgc ctcggcttac cttgcggcga tggccctgct gggcgtggcg cccggcgtcg
     2521 ccagaccgac g
//
LOCUS       DATLCO010000093         8535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8248303, whole genome shotgun sequence.
ACCESSION   DATLCO010000093 DATLCO010000000
VERSION     DATLCO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8248303"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VMZ33_02310"
     CDS             <1..700
                     /locus_tag="VMZ33_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HUR16095.1"
                     /translation="KVRVLRAIETNWTEARARAQVVRGQYTSGWVGDKHVAGYREEEE
                     VDAKSKVESFVALKLEIQNWRWADVPFYLRTGKRMPRRATEIAIQFKLPPLMLFGDAS
                     TPPEANLLAMRIQPDEGIMLRFAAKVPELGLDVRSVNMDFTYGMSFGHDAPEAYETLL
                     LDAMLGDASLFTRADEVEAAWSIVTPIHEIWREWDAKADDPDAEMCFYEAGTWGPERA
                     DELIEHDGRRWRRL"
     gene            722..2023
                     /locus_tag="VMZ33_02315"
     CDS             722..2023
                     /locus_tag="VMZ33_02315"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HUR16096.1"
                     /translation="MTDSRSPSPLAPPAPATDTHLPAIETDVSRWHVKANSIKDCVEQ
                     LSDIWTTAAEQAEANPSADALEHALGDPRMAGHIDEHERQPSVRVRMRTSVLTLVVIA
                     PRPETAERALAAINALNQRHPSRAIVVSPGDFDGPATMNAHIYAACKLSERSGAEMCT
                     EEILIKTGGELSQHLSRVVTPLLIHDLPVVLWWPDDPPFGSRQFNEVVGTADRLLVDS
                     GSFHEDGGKRLAGMATVVSSGTSVSDVGWLRLTLWRELMAGLFDHPLLMPELEHIRHV
                     RIDIASPSSTLRITKAAFYCGWLASRLGWQVKEPLGPSNKADDDTLRGSFRDAKRREI
                     QVELRPVRVTLDGSKRAPGSLVRVEVEAQRPKATVRARITRQSDHLLATADWNSAQVT
                     RRAGRLEPFDETPFVAEALERRGQDRIFEGALTRAVRFVGS"
     gene            2043..2891
                     /locus_tag="VMZ33_02320"
     CDS             2043..2891
                     /locus_tag="VMZ33_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HUR16097.1"
                     /translation="MGTLIVGGAEIFVLRDKEAVARAAADLTIAALDAALQARGAAHW
                     VLTGGSSAVTLYSLLPQDEWRARIEDWSFVHLWWGDERMVPPDHPESNVGLAYRALLA
                     IGAKAAQSSGGEGTDVGAGVTAGLPIHPNNIHAPSVMESLASDDPGSFAAEKYAAAVQ
                     QLAPTGLDGNPEFDLIHLGVGPDGHILSVFPGSPALAPDAPLAMPIAAPEHVSPHIPR
                     LTLTPRILAPAQRIIVMATGSAKAGVVAAILGQDRQPGRWPAQAVLLPNATWLIDAEA
                     AAQLPH"
     gene            complement(2880..4199)
                     /gene="chrA"
                     /locus_tag="VMZ33_02325"
     CDS             complement(2880..4199)
                     /gene="chrA"
                     /locus_tag="VMZ33_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HUR16098.1"
                     /translation="MVDDEAGSDATPKPTFGEASRTWGYVALNSFGGPAGQIAVLHRE
                     IVEKRGWIDEKRFLHALNYCMLLPGPEAQQLATYIGWLLHGVRGGLVAGLLFILPGVV
                     ALLTLSVIYAGFRDTTLVQALFYGIKPAVIAVVALAVYRIAKRALISRTHVVIAIAAF
                     LALYLFDVPFPAVIAAAAVAGVVAVRRGAIPIDDDLRPTDRPPARDSIAALLTGLVIW
                     VAPTLAVYLIFGPSSIFTQLTLFFSFAAVITFGGAYAILAFVAQQAVEVFGWLTADQM
                     LDGLGLAETTPGPLIMVLQFVGFMAAFAAPTGMDPFVAGALGGLLVTWVTFVPSFTFI
                     FSGAPYAEYVRNRPNLSSALSGITAAVVGVMLNLALFFAVHTLFGSTGEVQYGPVSLP
                     TVDLATLDVFGLGLAIAAFVALFRYKAPLLWTLGLSAGAGLVYFLVR"
     gene            4289..5170
                     /locus_tag="VMZ33_02330"
     CDS             4289..5170
                     /locus_tag="VMZ33_02330"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HUR16099.1"
                     /translation="MIGRLLSRLVYANAGWATPLGNFNVRVLRAIYRPIRPIKDFLNG
                     KWLGHSIHGALTDIPIGAFTLVILFDLLNLRTAADIALLIGVLVMVAAAVAGTADYSD
                     TDDEPRMVATVHATLMTTALVVYVISVVLRMGTGETGDRTVAVILSFVGYAIVTAGAF
                     VGGELVYTLGNMVNRHAWRFFGTPKWTKLDVTDIAEGVPTAAKAGSQQLVVVRTGSAV
                     YALHAQCAHAGGPLAEGKLVDGCIECPWHYSRYRMSDGKRTQGPTTFDQPRYDVRAAE
                     GGGWEVLRVTGERGQNP"
     gene            complement(5167..6111)
                     /locus_tag="VMZ33_02335"
     CDS             complement(5167..6111)
                     /locus_tag="VMZ33_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HUR16100.1"
                     /translation="MARGRALIGPGAFFGLASAFSWGIGDFCGGLVSRWSSVLSAMLV
                     SQGLGFAAMAAILGIVGEPAPTQDAVIWASLAGVSGVVGLWFFYFALARGTMGVVAPL
                     AALVGAGVPVLVAVANGEQLSPGRLLGIGLALLAVILISVPGPVSSDGERRSRRADLA
                     DMPYAVISGLGFAGFFIFVDIASANGATWWPLTIVRAAGLALVAVAIIWATARAKGAD
                     LPARLRFTLGVDRMRAHGRGLTALLPLLLLTGIGDVGGNVFFLLASHSDALSVAVVLA
                     SLYPVVTTLLAVLILRERLSRIQLLGVILAMASVPLLR"
     gene            complement(6090..8417)
                     /locus_tag="VMZ33_02340"
     CDS             complement(6090..8417)
                     /locus_tag="VMZ33_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR16101.1"
                     /translation="MHISRLHLQNFRRHSDLTLDLAPGLNVVRGPNEAGKSTVQRAIE
                     MALFRRPTSSSQDLDDSRQWRQPDSDPTVELDFAADEERGSLRKVFAGHRGTVELRIG
                     AETIVDPAAVEAHMARLTGLPSEKFFRATASVHHQELTGLTQDESTLRDRLQQSMSGA
                     DRGTHAARRKLEEAIRRYRTEGVKNPGYLKVLRTDVERLREQVRRGEQALEQLERDRR
                     ILAEARALRAAQDERLAEQRENVARAERAVTLTTKIADSTRRYAVYKRAAELRGEMNQ
                     LQASHPSTIPLPTLKATVEYLRNREFNLSEMRAELAAEPDLSGYDAALVEPRWRPLLT
                     AAAVLFVMAALAAIAGVVLVQPVIGLITAVAVGVLAAIAAFAGVGRRRRVEDVRMQNE
                     LRESEIARRLAGRTDLAERVGQAEQQRAEALASIGLSDLSRAETMLAAEVEHAAQIGA
                     RKAEYRGLLGDDPADEDVSVLRDQAAAEADESRHALAGMGDVGREPEKHLAVYVIAVQ
                     KLTSERDTTLQQEATAEARVTANEVDAEVVAADAEALGSTQESLASAERRLRIYEDVL
                     ATLNDAERTTMKKAARFLEQRMARDVEKVTGGRYRRLKVDEQTLTFTVFSPETNDWLD
                     VRQLSQGTLDQLYLCARLGIVRQVTSPAAPPLIFDDPFITFDETRAARAVGLLKELAR
                     DNQVIYLTTSDRYDAQADKVIELPEPLDRDEPEPIATASGPTETLSFWSSSAIPVEPR
                     ANGHHGAPATAADAPGQPPVEPAPEPTPLWPVEER"
BASE COUNT         1467 a         2801 c         2930 g         1337 t
ORIGIN      
        1 gaaggtgcgt gtcctgcgcg caatcgaaac gaactggact gaagcccggg cgcgcgccca
       61 ggtcgtgcgt ggccagtaca cgtcgggctg ggttggtgac aagcacgtcg ccggctatcg
      121 cgaggaggag gaggtcgacg ccaagtccaa ggttgagagc ttcgtcgccc tgaagctcga
      181 aatccagaac tggcgctggg ccgacgtgcc cttctacctg cgcaccggca agcggatgcc
      241 caggcgcgcg acggagatcg cgatccagtt caagctgccg ccgctgatgc ttttcggcga
      301 cgcgtcgacg ccgcccgagg ccaacctgct ggcgatgcgc atccagccag acgagggcat
      361 catgctccgc ttcgcggcca aggtcccgga gctcggcctg gacgtccgca gcgtcaacat
      421 ggacttcacc tacggcatgt ccttcggcca cgacgcacct gaggcatacg agacactgct
      481 cctcgacgcg atgctcggcg acgcttcgct gttcacgcgc gccgacgagg tcgaggccgc
      541 gtggagcatc gtgacgccca tccacgagat ctggcgcgaa tgggacgcga aggccgatga
      601 tcccgacgcc gagatgtgct tctacgaggc cgggacatgg gggccggagc gcgccgatga
      661 gctgatcgag cacgacggcc gccgatggcg gcggctgtag ccgcgcgagg gcaccagcgc
      721 catgactgac agccgcagtc cttcgccgct cgccccgccg gcgcccgcga ctgacacgca
      781 cttgccggcc atcgagaccg atgtctcgcg ctggcacgtc aaggcgaaca gcatcaagga
      841 ctgcgttgag cagttgagcg acatctggac gaccgccgcg gagcaggctg aggcgaaccc
      901 atcggccgac gcgctggagc acgcactggg cgatccgcgg atggcgggcc acatcgacga
      961 gcacgagcgg cagccgtcgg tccgcgtgcg gatgcgaacc agtgtgctga cgctcgtggt
     1021 aatcgcgcca cggccggaga cagccgagcg cgctcttgcg gcgatcaatg ccctgaacca
     1081 gaggcacccg tcacgggcca tcgtcgtctc accaggcgac ttcgacggcc cggccacgat
     1141 gaacgcccat atctatgccg cgtgcaagct ctccgagcgc tccggcgccg agatgtgcac
     1201 cgaggaaatc ctgatcaaga ccggtggcga gttgagccag cacctctcac gcgtcgtcac
     1261 gccgctgctc atccacgacc tccccgtggt cctgtggtgg cccgacgacc cgccgttcgg
     1321 ctcgcggcaa ttcaacgagg tcgtgggcac ggcggatcgg ctgctcgtcg attccggatc
     1381 tttccacgag gacggcggca agcgcctggc cggcatggcc acggtggtat ccagcgggac
     1441 gtcggtttcg gacgtcggtt ggctcaggct gactttgtgg cgcgagctga tggccggtct
     1501 cttcgatcat ccgctgctca tgcctgagct cgagcacatc cgccatgtgc gtatcgacat
     1561 cgcgagtcca agctcgacgc tgcgcatcac caaggcggct ttctactgcg gctggcttgc
     1621 gtcccgtctg ggctggcagg tcaaggagcc cctggggccg tccaacaaag ccgacgacga
     1681 cacgttgcgc ggcagcttcc gcgacgccaa gcggcgcgag atacaggtcg agctgcggcc
     1741 cgtgcgtgtg acgctcgacg gctcgaagcg cgcgcccggc tcattggtgc gcgtcgaggt
     1801 cgaggcgcag cgcccgaagg caacggtcag ggcgcgcatc acccgccagt ccgaccacct
     1861 gttggccact gccgattgga acagcgccca ggtgacaaga cgtgctggac ggctcgagcc
     1921 gttcgacgag acgccgttcg tggccgaggc gctggagcgg cgcggccagg atcgcatctt
     1981 tgagggggcg ctcaccaggg ccgtgcgctt cgtcggaagc tagggaggcg tcggtggacg
     2041 ccatgggaac gctgattgtc ggcggggcgg aaatcttcgt tctgcgggac aaggaggcag
     2101 ttgcccgcgc ggccgcggac ctcacgattg ctgcactcga cgcggcgctc caggcgcgcg
     2161 gcgccgcgca ctgggttctg acaggcggct cgtcggccgt gacgctctat tcgctgcttc
     2221 cgcaggacga gtggcgcgcg cggatcgagg attggtcgtt cgtccacctg tggtggggcg
     2281 acgagcggat ggtgccaccc gaccatcccg agtccaatgt cggtctcgcg tatcgggcgc
     2341 tgcttgcgat cggggccaag gctgcccagt cgagcggggg cgaagggact gacgtgggcg
     2401 cgggcgttac cgcaggattg ccgatacacc ccaacaacat ccatgcgcca tcggtcatgg
     2461 agtcgctcgc cagcgatgac ccgggaagct ttgccgcgga gaaatacgcc gcggccgtgc
     2521 aacagctcgc gccaacgggg ttggacggca atcctgagtt cgacctcatc cacctcggcg
     2581 ttgggccgga cggacacatc ctgtcggtct ttccggggag cccagcactg gcacctgatg
     2641 cgccactggc gatgcccatc gctgcgccgg agcacgtgtc gccgcatatt ccgcgtctga
     2701 cgctcacgcc ccggatcctc gcaccggcgc agaggatcat cgtcatggcc acgggatcgg
     2761 cgaaggccgg tgtagttgcg gcgatcctgg gccaagaccg ccagccgggg cgctggccgg
     2821 cgcaagcagt gctccttccc aacgccacgt ggctgatcga cgccgaggcg gccgcgcagc
     2881 taccgcacta ggaaatacac gagtcccgcc cctgcggaga gaccgagagt ccatagcaag
     2941 ggcgccttgt agcggaacag cgcgacaaag gccgcgatcg ccaacccgag cccgaagacg
     3001 tcgagggtcg ccaggtcaac tgtcggcagg ctgaccgggc cgtactggac ttcgccagtc
     3061 gagccgaaca gcgtgtgcac cgcgaagaac agcgcaaggt tgagcatcac gccgacgacc
     3121 gcggcggtga tgccgctcag tgcagacgac aggttgggcc ggtttcggac gtactcggcg
     3181 tagggcgcac cgctgaagat gaaggtgaac gacggcacga atgtgaccca cgtcacgagc
     3241 aacccgccga gcgcgccggc tacgaacggg tccatgccag tcggcgcagc gaacgcggcc
     3301 atgaacccga cgaactgcag gaccatgatg agggggccgg gcgtcgtctc ggcgaggccc
     3361 aggccgtcga gcatctggtc ggccgtgagc cagccgaaca cctctaccgc ttgttgcgcg
     3421 acgaaggcca ggatcgcgta ggcgcccccg aacgtgatca ctgcggcgaa ggagaagaac
     3481 agcgtgagct gggtgaagat gctcgatggg ccgaagatca ggtaaacggc caacgtcggt
     3541 gccacccaga tgacgagacc cgtgagaagg gcggcgatgg agtcacgagc cggcgggcga
     3601 tcggttgggc ggagatcgtc gtcgatcgga atggcgccgc gtcgaacggc cactacaccg
     3661 gccactgcgg ctgccgcgat aacagccggg aacggcacgt cgaagaggta gagcgccagg
     3721 aacgccgcga tggcgataac gacgtgcgtg cggctgatca gcgcacgctt cgcgatgcgg
     3781 tagacggcca gcgccacgac tgcgattacc gccggcttga tgccgtagaa gagggcctgg
     3841 acaagtgtcg tgtcgcggaa gccggcatag atgactgaca gcgtgagcag cgcgaccacg
     3901 ccgggcagga tgaagagcag acccgccacg agcccgccgc gcactccgtg aagcagccag
     3961 ccgatgtacg tcgccaactg ctgcgcctcg ggaccgggga gcaacatgca gtaattgagt
     4021 gcatgcagga atcgcttctc gtcgatccag ccacgcttct cgacgatctc gcggtgcagc
     4081 accgcgatct ggcctgccgg gccgccgaag ctgttcaggg cgacgtagcc ccacgtgcgg
     4141 cttgcttcgc cgaaggtcgg cttgggggtg gcgtcggagc cggcttcgtc gtcgaccatc
     4201 ttcaccggaa tggtaggtaa tgacgcccat tgggtgtggc gtttggtgtg gcatcggcat
     4261 acgctccgcc aaaagacgga ggtgtcggtt gatcggtcgg ctgctctcgc ggcttgtcta
     4321 tgccaacgcg ggctgggcaa cgccactcgg caacttcaat gtgcgcgtgc tccgcgcgat
     4381 ctatcgaccg atacggccga taaaggactt cctcaacggc aagtggctgg ggcactcgat
     4441 ccacggcgcg ctgacggaca tcccgatcgg cgccttcacc ctggtcatcc tttttgacct
     4501 tctcaatcta cggaccgcgg ccgacatcgc gctcctgatc ggtgtgctgg tcatggttgc
     4561 ggcggccgtc gccggaacgg ccgactacag cgacacggac gacgagccgc gcatggtcgc
     4621 cacagttcac gccacgctga tgacgacggc gctggtcgtc tacgtcatct cggtcgtgct
     4681 gcgcatgggc acgggcgaaa cgggcgaccg gaccgtggcg gtcatcctct cgttcgtcgg
     4741 ctacgcgatt gtcaccgcgg gtgcgttcgt cggtggggag ctcgtctaca cgcttggcaa
     4801 catggtcaat cgccacgcct ggcgcttctt cggtacaccc aagtggacca agctcgatgt
     4861 cactgacatc gcagaaggcg tgcccacggc agcgaaggcc ggttcccagc agctcgtggt
     4921 cgtgcgcacc gggtctgcgg tttacgcgct gcacgcgcag tgcgctcacg ccggcggacc
     4981 gctcgcggaa ggaaagctcg tcgacggctg catcgagtgc ccgtggcact actcgcgcta
     5041 ccggatgtca gacgggaagc gaacccaggg gccgacgacg ttcgaccagc cgcgctacga
     5101 cgtccgtgcc gcagagggtg gcggctggga ggtcctgcgt gtgaccggcg agcgcggcca
     5161 gaacccctag cggagaagcg gcacgctcgc catcgccagg atcacgccca gcagctggat
     5221 ccgcgacaac cgctcccgaa ggatgagcac cgccagcagc gtcgtgacca cggggtaaag
     5281 cgaggccagg accaccgcca cggacagcgc atcgctgtgg ctggcgagga ggaagaagac
     5341 gtttccgccc acgtcgccga tgccggtgag tagcagcaag ggcaacaaag ccgtgagccc
     5401 acgcccatgc gcccgcatcc ggtcgacgcc gagcgtgaag cggagccgcg cggggagatc
     5461 ggctcctttc gctcgcgccg tcgcccagat gatcgcaacc gcgacgagtg ccaggccagc
     5521 ggcccgtacg atcgtcaagg gccaccaggt tgccccgttg gctgatgcaa tgtcgacgaa
     5581 gatgaagaac cccgcaaagc ccaggcccga aatcacggcg tagggcatgt cggccaggtc
     5641 agcccgtcgc gatctgcgtt ccccgtcgct ggaaactgga cccgggacag agatgaggat
     5701 gactgccagg agggccagcc cgatgccgag cagccgacct ggcgacagtt gctcaccgtt
     5761 ggccacggcc accagcacgg gcacgccggc tccgaccagc gcagcaagtg gagcgacgac
     5821 gcccatggtc ccgcgtgcca gagcgaagta gaagaaccac aggccgacaa cgcccgacac
     5881 ccctgcgagg gacgcccaga tgaccgcatc ctgggtcggc gcgggttcgc caacgatccc
     5941 gaggatcgcc gccattgcgg caaaaccgag accctgactg acgagcatcg cgctcaggac
     6001 gctcgaccag cgggatacga gcccaccgca gaagtcgccg atcccccacg agaacgcgga
     6061 agcgagcccg aaaaaggcgc ctgggccgat cagcgctctt ccacgggcca gagcggcgtc
     6121 ggctctggag caggttcgac tggcggctgc cctggggcat cggccgccgt ggccggggct
     6181 ccgtgatggc cgttggcacg aggttcgacg gggatagccg agctggacca gaacgaaagc
     6241 gtctcggtcg ggccgctggc tgtcgcgatc ggttccggtt cgtccctgtc gagcggctcg
     6301 ggcagctcga tgaccttgtc ggcctgggcg tcataccgat cggacgtggt cagatagatc
     6361 acctggttgt cgcgcgccag ctccttgagc aggccgaccg cgcgggcagc gcgcgtctca
     6421 tcgaaggtga tgaagggatc gtcgaagatg agcggcggtg ccgccggaga cgtcacctgg
     6481 cgaacgatac ccagccgagc gcaaaggtag agctgatcga gcgtgccctg cgagagctgg
     6541 cgaacgtcga gccagtcgtt cgtctctggt gagaagacgg tgaacgtgag tgtctgctcg
     6601 tcgaccttca ggcggcggta acggccgccg gttaccttct cgacgtcgcg cgccatgcgc
     6661 tgctcgagga agcgtgccgc tttcttcatc gttgtgcgct cggcgtcgtt gagtgtcgcc
     6721 agcacgtcct cgtagatgcg caggcgacgc tccgccgatg ccagtgattc ctgggtcgac
     6781 ccaagcgcct cggcgtcggc ggcgacaacc tcggcatcta cctcgttggc agttacgcgc
     6841 gcctcggccg tggcttcctg ctggagagtg gtgtcacgct ccgaagtgag cttttgaacg
     6901 gcgatgacgt ataccgccag gtgtttctcg ggctcgcggc caacgtcgcc catgcccgcc
     6961 agcgcgtgtc ggctctcgtc ggcctcggcc gctgcctggt cgcgcaggac gctgacatcc
     7021 tcgtcggctg ggtcgtcgcc cagcagaccg cgatactccg ccttgcgggc gccgatctgc
     7081 gccgcgtgtt caacctctgc agcgagcatc gtctcggcac gcgatagatc cgacagcccg
     7141 atggatgcga gagcttcggc gcgttgctgc tctgcctggc cgacgcgttc agcgagatcg
     7201 gttcgaccgg cgagccggcg cgcgatctct gattcgcgta actcgttctg catgcggacg
     7261 tcctcgaccc gtcgacgccg cccgactcca gcgaaagcgg cgatggccgc caggactccg
     7321 acggcgacgg cggtgataag cccgatgaca ggttgcacaa gcacaacgcc cgcgatcgcg
     7381 gcgagcgctg ccatcacgaa gaggaccgcg gcagctgtga gcaacgggcg ccagcgtggt
     7441 tcgacaaggg cggcgtcgta ccctgacagg tccggctcgg cagcgagctc ggcccgcatc
     7501 tccgacaggt tgaactcgcg attgcgcagg tattccacgg tcgccttgag cgtcggcagc
     7561 gggatcgtgg agggatggct cgcctggagc tggttcattt cgccacgcag ctcggcggcg
     7621 cgtttgtaga cggcgtagcg cctcgttgaa tcggcgatct tggtggtcag ggtcaccgcg
     7681 cgttcggcgc gcgcgacgtt ttcgcgctgc tcggccaggc gctcgtcctg cgcggcacga
     7741 agagcgcgtg cctcggccag gatccgccgg tcgcgttcga gctgctccag cgcctgctcg
     7801 ccacgccgca cctgctcgcg cagccgctcg acgtcagtgc ggaggacttt gagatagccg
     7861 gggttcttga caccctcggt ccgatagcga cggatcgcct cttccagctt gcggcgcgcg
     7921 gcgtgcgtgc cgcgatcggc cccgctcatc gactgctgta gccggtcgcg cagcgtgctc
     7981 tcgtcctgcg tcaggccggt cagctcctga tggtggacgc tggccgtggc gcggaagaac
     8041 ttttcgctcg ggaggcccgt gagccgcgcc atatgagcct ctaccgcggc cgggtccacg
     8101 atcgtttcag cgccgatccg cagctccacg gttccgcggt gcccggcgaa aaccttgcgc
     8161 aggctgccgc gctcctcgtc ggcggcaaag tccagctcaa cggtcggatc ggagtccggt
     8221 tggcgccact ggcgggagtc gtcgaggtcc tgagacgagg acgtcggacg acggaagagc
     8281 gccatctcga tggcccgttg aactgtcgat tttcccgcct cgttggggcc gcgaacgacg
     8341 ttcaggccgg gcgcgaggtc gagcgtcagg tcggagtggc gcctgaaatt ctgcaggtga
     8401 agccggctga tgtgcatcgc taggccagcg tgacgcgctg cggatcgtcg agcagcaaga
     8461 tcccgagtcg cagaccctca cgcagctccg ccgcgcgttc gtgctcgccg cgactctcgt
     8521 ggtcaacgat gcgcg
//
LOCUS       DATLCO010000094         9299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4570078, whole genome shotgun sequence.
ACCESSION   DATLCO010000094 DATLCO010000000
VERSION     DATLCO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4570078"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VMZ33_02345"
     CDS             complement(<1..284)
                     /locus_tag="VMZ33_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HUR16102.1"
                     /translation="MSEEGLSGSVATGPSHRVEFRVLGPVEALKNGQPVALGGPRQRT
                     LLALLLLRPGRTVPVDELLDAIWAGEPPAGAPTTIRSYVSRLRAALGDGA"
     gene            complement(365..2005)
                     /locus_tag="VMZ33_02350"
     CDS             complement(365..2005)
                     /locus_tag="VMZ33_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16103.1"
                     /translation="MVEADGSFFGATGLAGTFGGGGWALGSDLASGEPPILEPGGASV
                     TATAVVGTVYALAVSGNNLYVGGEFENVAGIPEADYIARWDGTAWSALGSNGAGNGAL
                     DAGVRAIAIHGSNVVVGGFFNNVAGIAGADYVARWTGTSWAPLQNLCCGAAAINNSVH
                     ALAVQGDFVYAGGYFTDAGGFTGVNYIARWSVPCDCWSAVGPAGSMTGPVFAIGLGAS
                     RLYVGGLFDNAGGKQFADNVAFLSGNEWWHMGSGVNGPVFALLVFRPTEFDEHVYVGG
                     GFTNVGGATGDYLARFDSLDWYPVGSNGAGDGALNDSVYTLTLVNGSLFVGGTFEDAG
                     GNVNADSGARWSGSAWFPLAAGFSALNSHVFALAPSSNGLYIGGHFTDAGGQAEADRV
                     VLRKGSEWIPLSPRRPDGRIRLRNGAFVGNNVYNGTGVNQTRTGSAAPGQVIMFGISA
                     QNDSAIADRIRVGATGTSVSGYTIKYFTGSTDVTLAVNSGTYQTPVLPAGGSVLITAK
                     VTVKQSAADGSSISRLVTLRSVADQTKLDVLKLVAQRT"
     gene            complement(2281..3483)
                     /locus_tag="VMZ33_02355"
     CDS             complement(2281..3483)
                     /locus_tag="VMZ33_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUR16104.1"
                     /translation="MSFEPPTPAIDARAKQVSRNADMRGVAEALVAQRAAILDGWQNV
                     ATRQPFHAGHPDRAIADHIPALFDALTDLLSRDIPGEDIEAPMDDAAVAAAARAHAQM
                     RFEQSLGPVAIATEFRLLRQEISRALRVQLEEDLSIDDVVSSLAVVNDALDGATNLAL
                     SALTDRIETVREEFLATTLHDVRQPIVLIQASLVLAARWARREPLDLEQLTETLDGAL
                     VATQEMSHLVETLADASRAAMGALDLIPEPVVVREIIKDALDTLDATGRRRVTVDSSL
                     ASGAVVYWDRQSIRRVLTNLLTNALKYSPPESPVTVTVTQDVANIEVTVRDHGMGLLP
                     DEKAILFQRYSRTAEARTRGLPGVGLGLFACKGLVEAHGGRIALESDGRDQGTTARIL
                     LPIGKPDS"
     gene            complement(3587..5572)
                     /locus_tag="VMZ33_02360"
     CDS             complement(3587..5572)
                     /locus_tag="VMZ33_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUR16105.1"
                     /translation="MSSDWPVFGRDVPDAAWRPPRDSAANTRLGRFLRAAGEPDLEAL
                     QRHAERDPAWFWGSAIDDLGFGWQRQPTSILDSSGGIEWSRWWTGGAFNYAAAAVDPR
                     ATTNPDGPAVTWVGEDDDVRRLTNADLKAQVDRAAAMFARLSVGEGDRVGIFLPLLIE
                     TVITVLALGKLKAIYIPIFSGYAAPAVASRLNDAGATLLVTADGTLRRGAKVALKDTA
                     DAAVEQAPTVRNVLVVDRLTRSTPHSDRGLGRDRWWSIEMADPDLVPLTETPETHPET
                     PYMIIYTSGTTGRPKGAVHVHGGFPIKGAQDLAHCFDLGPGDRLFWFTDLGWMMGPWA
                     ISGALLLGAELVLYEGAPDHPGPDRLWATVARHGVTHLGLSPTVIRALMAHGVEHVRR
                     HDISSLRVLGSTGEPWNPESWWWFFNNIGGGQLPLINYSGGTEVSGGIVSCNLMTPIK
                     PTSFGGPSVGTAADVIDADGKPVRGEVGELVVRQPQPGMTRGFWQDAHRYVETYWSRI
                     PGTWVHGDWAVVDADGYWFIQGRSDDTLKVAGKRVGPAEVESAAASHKSVLEAAAVGV
                     PHDIKGETIVVFVRLRPGETDDPDLRQAVSAKVVEQLGKTLKPEAIHVVSDLPRTRSG
                     KLMRRVARAAYLGTDPGDLSALENPLSLEDIRSAARP"
     gene            complement(5597..6040)
                     /locus_tag="VMZ33_02365"
     CDS             complement(5597..6040)
                     /locus_tag="VMZ33_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16106.1"
                     /translation="MRRLALVALSIIVLACSGGAIAEPTETPGTMDDAIGALVLNGVA
                     VHNLVGGDAGCPEQDLHDNALRLEVSIGTMSSTRTIYLMRWRRQTDFDAAAESFAACI
                     TEFGELSGATQIDQVANAPWRAYGANWPPQLLLAVTDALESVGSQ"
     gene            6336..7094
                     /locus_tag="VMZ33_02370"
     CDS             6336..7094
                     /locus_tag="VMZ33_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUR16107.1"
                     /translation="MALERMPRYDVSNDGMGPYAIFYCDRDGREYRSTPNIATTVKEN
                     VTRGALGGFLRNVPILGDQAANQVDNDRYRTDMSADELNSAWNATKQYFRSCPTCSQI
                     VCVPDFDEPTGFCKEDSPRRAEVEAAQAQQSAAALKGLADVFGISGAIKGGMAAAQAQ
                     SEAGMATCPKDGTKAAAGTGFCPNCGSVMTQPAPVAQASTSACPSCGTAMAAGVKFCP
                     NCGTPAAQPKACASCGTEMAAGVKFCPNCGTPAT"
     gene            complement(7142..7660)
                     /locus_tag="VMZ33_02375"
     CDS             complement(7142..7660)
                     /locus_tag="VMZ33_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16108.1"
                     /translation="MPPDLERLANYARSRGFRRYVAITLLVIGCVPALLIVLLRPPVG
                     QGTILVAALLIFTPAALLLLWTVRDGEGQAAREVVMWLRVIDRAVTSAPWLVPYKELP
                     AVPDDSELPRVRMGDLAVDLRETISPLVASGGLTFRILVQRFWYPLQALALGLLLAVA
                     LTVVLPNFSRSG"
     gene            complement(7687..8238)
                     /locus_tag="VMZ33_02380"
     CDS             complement(7687..8238)
                     /locus_tag="VMZ33_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HUR16109.1"
                     /translation="MAEQFLSQTKALLQRTPRVLSALLGGLPDVWLEGRDTPDGWQPR
                     DVVGHLITAELDDWIPRVRMILDYATTRAFDKFDRHAMLERDREVPMDALLKRFGELR
                     AQSLRELDELVGDADLEKVGTHPGLGDVTMRNLLATWAVHDLDHTSQVFAGMSAAHDA
                     TVGPWKGYLGILLRREDPSAIPG"
     gene            complement(8247..>9299)
                     /locus_tag="VMZ33_02385"
     CDS             complement(8247..>9299)
                     /locus_tag="VMZ33_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16110.1"
                     /translation="VLVSTRDDPVLAQWQYGLGRSVAWTSDAQGRWATNWVAWTGFNQ
                     FFSQLVSWTFPGEESGGIEAEFVTQGDDTRLRLRSVESDGTPRNFYDTIVNITDPNFE
                     LLEGLRLDQVAPGVYEADLGSLTPGAYALRFNQMKPGTTPLARTVMLIAPPPAEYRLL
                     GTNERLLAALRSATGGQALVAPEEPWEHDLGTTTAATDMVPWLLLLGLVLWPLDVAVR
                     RVSLQRGDLGLARAWAGAHWRSWRGPARRTRQVGEMLAAKERAGGASARAALFRTPDA
                     ETDTTATATVTGPKPVRPQAGPAPIKTAAPPQPVAATAAAPTATPTPTQPPAAPAPTD
                     TIARLREAKQRARDSR"
BASE COUNT         1597 a         3095 c         3075 g         1532 t
ORIGIN      
        1 atcgcgccgt cgcccagggc cgctcgcaga cgcgaaacat aagaccggat ggtggtcggc
       61 gcacccgctg gcggctcgcc cgcccagatc gcgtccaaca gctcgtcgac cggcacggtt
      121 cggccggggc gcagcagaag aagcgccagt agcgtccgct gtcgcgggcc gccgagcgcc
      181 accggttggc cgttcttcag cgcctccacc gggcctagca cgcgaaactc gactcggtgg
      241 ctcggtcccg tagcaaccga gccggagagg ccctcctcgc tcacgagagc gaagcctaag
      301 ggtcacgcca aatcagcgac cccgtttgcc tcatgcttgg ctgcgccggc agaactgtgg
      361 ccggtcaggt ccgttgtgcg accagcttga ggacgtccag cttggtctga tcagccaccg
      421 agcgcagggt cacgagccgg ctgatactcg aaccgtcggc ggcactctgc ttgaccgtca
      481 ctttggcggt aataaggacg cttccgcccg ctggtagcac gggcgtctga tacgtgccag
      541 agttgacggc tagggtcacg tcggtgctcc cggtgaagta cttgattgtg taaccgctga
      601 cgctggtccc tgtcgcccca accctgatgc ggtcggcgat agcgctgtcg ttttgcgccg
      661 agattccaaa catgatcacc tggccggggg ccgccgagcc ggtccgggtt tggtttacgc
      721 ctgtgccgtt gtagacgttg ttgccgacga atgcgccgtt cctgaggcgg attctgccgt
      781 ccggccggcg aggcgacaac ggaatccatt ccgagccttt tcgaaggacg actcggtctg
      841 cctctgcctg gccacccgcg tccgtgaagt gcccgccgat atagaggccg ttgctactcg
      901 gtgccagcgc gaacacgtgc gagttcagcg ccgaaaaacc tgcggccagc gggaaccacg
      961 cgctcccact ccagcgcgca cccgaatcgg cgttcacgtt tccgccggcg tcctcgaacg
     1021 tgcctccaac gaacagcgat ccgttgacga gggtgagtgt ataaaccgag tcgttcagcg
     1081 ccccgtcgcc ggcaccgttg gacccgaccg gataccaatc gaggctgtcg aaccgggcga
     1141 ggtagtcgcc ggtcgctccg ccgacattgg tgaagcctcc gcctacgtag acgtgctcgt
     1201 cgaactcagt cggccggaac acaagcagcg cgaagacggg tccgttgacc ccgctgccca
     1261 tgtgccacca ctcgttgccg ctgaggaatg cgacgttgtc agcgaattgc tttccgcctg
     1321 cgttgtcaaa caggccgccc acgtagagcc ggctggcgcc cagcccgatg gcgaagaccg
     1381 gaccggtcat cgagccggcg ggaccaaccg cggaccagca gtcgcatggt acggaccagc
     1441 gggcgatgta attcacgccc gtgaagccgc cggcatcagt gaagtagccg cctgcgtaca
     1501 cgaagtcccc ctgtaccgcg agggcgtgga cggagttgtt gatcgcggct gccccgcagc
     1561 acaggttttg caacggcgcc cagctggtcc cggtccaacg tgcgacatag tcggctccgg
     1621 cgatcccggc cacgttgttg aagaagccgc cgaccacgac gttagaaccg tgtatcgcaa
     1681 tagcacggac gccagcgtca agagcgccat taccggcacc gtttgagccg agcgccgacc
     1741 aggccgtccc gtcccagcgc gcgatgtagt cggcctccgg aattccggcg acgttttcga
     1801 actcgccgcc gacgtacaga ttgttgccgc tcaccgccag cgcgtagacg gtacccacta
     1861 ctgctgtcgc cgtaactgac gcgccacctg gctcaaggat gggcggctcg ccgctggcaa
     1921 ggtcgctgcc gagcgcccac ccgccgccac cgaaggtgcc ggccagcccg gtggcgccga
     1981 agaagctgcc gtctgcttca accaccagcc tcggatcgaa ggtgcccgcg ttggggccgg
     2041 aagtgctcaa acccgtgtcg ccagaagaac tggccgatgg cgcctcggct gccgcgccag
     2101 gcgatggccc ggccagaccg accaagaggc agatcgcgat taaggttcca gcctgcttca
     2161 tgctcagtct cccgtcgtgc gatcgcacga cgcggtgcca gccgcgcgcg tcgaacccga
     2221 gcaggttcgt tcccagcggt tgcaatccgg ttgcagccgc gtggcgctga gggtggagcg
     2281 tcagctgtct ggcttgccga tcggcagcag gatgcgagct gttgtgcctt gatcacggcc
     2341 gtccgattcg agtgcgatgc ggccgccgtg cgcttcgacc agacccttac aggcgaaaag
     2401 ccccagaccg acgcccggca gcccgcgggt gcgagcctct gccgttcgac tgtagcgctg
     2461 gaagagtatg gctttctcat cgggcagcag gcccatcccg tgatcgcgga cggtgacctc
     2521 gatattcgcg acatcctgtg tgactgtgac ggtcacgggc gactcggggg gtgagtactt
     2581 gagtgcattc gtcaggaggt tggttagaac acgcctgatt gactgtcgat cccagtacac
     2641 gacggcgccg ctggcgagcg acgaatcaac cgtaactcga cggcggccgg tggcatcgag
     2701 cgtgtccagt gcatccttga tgatctcgcg gacgaccacc ggttctggaa tcaggtccag
     2761 cgcacccatc gccgcgcgcg atgcatcagc caacgtttcg acgaggtgcg acatctcctg
     2821 agtcgcgacc agagcaccgt cgagcgtttc ggtcagttgc tcaaggtcca acggttcacg
     2881 ccgtgcccag cgtgccgcca acacgagcga ggcctggatg aggacgattg gctggcgcac
     2941 gtcgtgaagc gtcgtcgcca ggaattcctc gcggacagtc tcgatccggt cggtcagggc
     3001 cgacagcgcc agattggtgg cgccgtcgag ggcgtcgttc accacggcca acgagctcac
     3061 tacgtcatcg atcgacaggt cttcctcgag ctggacccgc agcgcgcgag atatttcctg
     3121 gcgcaggagc cggaactccg tcgcgattgc caccggacca agactctgct cgaacctcat
     3181 ctgagcgtgc gcccgtgcgg ctgctgctac cgcggcatcg tccatcggtg cttcgatgtc
     3241 ctcgccgggt atgtcccgag aaagcaggtc ggtcaaggca tcgaaaagag ccggaatgtg
     3301 gtcggcgata gcccgatcag gatgccccgc gtggaacggc tgtcgcgtcg cgacgttttg
     3361 ccaaccgtca agaatggcag cgcgctgtgc cacgagggcc tccgccacgc cgcgcatatc
     3421 ggcgttcctt gagacctgct tggctcgcgc gtcgattgcc ggcgtcggtg gctcgaacga
     3481 catgcacggc tccttgttcg agacagacag gcgagttagg ggcggcggct gatgataacg
     3541 ccgtcgggcg accgcgaggc gctgcgccgc cgcggactcg ggtgccctaa ggacgcgccg
     3601 ccgagcggat gtcctccagc gagagtgggt tttcgagcgc cgagaggtca cccgggtcgg
     3661 tcccaagata tgccgcgcgc gcaacccggc gcatcagctt gccggaacgc gtccggggca
     3721 ggtcgctcac gacatggatc gcctcaggct tgagcgtttt gcccagctgc tcgacgacct
     3781 tggccgagac ggcctggcgg aggtccggat cgtctgtctc gcccgggcgc agccgcacga
     3841 aaacgacgat cgtctcgccc ttgatgtcgt ggggcacgcc gacggctgcc gcctcgagca
     3901 ctgacttgtg cgacgcagcg gcgctttcga cttcagccgg ccccacgcgt ttgccggcaa
     3961 ccttcagcgt gtcgtccgag cgaccctgga tgaaccagta gccgtccgcg tccacaaccg
     4021 cccagtcgcc gtgaacccag gtgccaggga tccgtgacca gtacgtctcg acataccgat
     4081 gtgcgtcctg ccagaagcca cgggtcatgc ccggctgtgg ctgacggacc accagctcgc
     4141 cgacttcgcc acgcacgggt tttccgtctg cgtcgatgac gtcggccgcc gtcccgaccg
     4201 acggcccgcc gaaagacgtt ggcttgatcg gcgtcatcaa gttgcaggac acgatgccgc
     4261 cgctcacttc ggtgccgccg ctgtagttga tgagtggcag ctggccgccg ccgatgttgt
     4321 tgaagaacca ccaccacgac tctggattcc atggttcgcc ggtcgacccc aggacgcgca
     4381 gtgaggagat gtcgtgccga cggacgtgtt ctacgccgtg cgccatgagc gcccggataa
     4441 ccgtcggtga gaggccaagg tgggtgaccc cgtggcgtgc gactgtcgcc caaagtcggt
     4501 cagggccggg gtgatcgggc gcgccctcgt agagcaccag ctcggcgccg agcagcagag
     4561 cgccgctgat cgcccacggg cccatcatcc agccgagatc ggtgaaccag aacagccgat
     4621 cgccaggccc caggtcgaag cagtgcgcca ggtcctgcgc gcccttgatg gggaagccgc
     4681 catgcacgtg aaccgcgcct ttcgggcgac ccgtcgtgcc cgacgtgtaa atgatcatgt
     4741 agggcgtctc gggatgggtc tcgggggttt cggtgagcgg aacgagatcg ggatccgcca
     4801 tctcgatcga ccaccagcgg tcgcgaccca gaccgcggtc agagtgcggc gttgagcgag
     4861 tgagcctgtc gacgacgagg acgttccgga cagtaggcgc ctgttcgact gcggcatcgg
     4921 ctgtgtcttt cagcgctact ttggcgccac gtctgagtgt gccgtcggcc gtgacaagca
     4981 aggtggcacc ggcgtcgttg agcctgcttg caaccgcggg ggcggcgtag ccggagaaga
     5041 tcgggatgta aatcgctttc agctttccaa gtgccagaac cgtgatcacg gtctcgatca
     5101 gcagtggcag gaagatgccg acgcggtcgc cctcgcccac gctcagtcgg gcgaacattg
     5161 ccgcggcgcg gtcaacctgt gccttgaggt cggcgttggt cagccgccgg acgtcgtcgt
     5221 cttcgccgac ccacgtgacg gctgggccgt ccgggttggt tgtggcgcgc gggtcgactg
     5281 ctgccgccgc gtaattgaag gccccgccgg tccaccagcg tgaccactcg atgccgccac
     5341 tcgaatcgag gatggacgtt ggttggcgct gccagccgaa ccccaggtca tcaatcgcag
     5401 agccccagaa ccatgccggg tcgcgctcag cgtgacgctg aagtgcctcg aggtccggct
     5461 cgcccgcagc tctaaggaac cggccaagac gtgtgttcgc ggctgagtcg cgcggcggtc
     5521 gccaggcggc gtcggggacg tcgcggccga agacgggcca gtcgctactc acggcagggc
     5581 atcgtagtcg cgccgactac tggctgccga ccgattcaag tgcgtcggtc accgcgagga
     5641 gcagctgagg gggccaattc gctccatagg cacgccacgg ggcgttggcc acctggtcga
     5701 tctgcgttgc tccactgagt tcgccgaact cggtgatgca agctgcgaac gactcggctg
     5761 ccgcgtcgaa gtcggtctga cgtcgccaac gcatcaggta aatggtccgc gtcgagctca
     5821 tcgtcccgat cgagacctcc aggcgaagcg cgttgtcgtg caggtcctgc tccggacaac
     5881 cggcgtcgcc gccgacgagg ttgtggaccg ctacgccgtt gagcacaagc gcgccgatcg
     5941 cgtcatccat cgtgcccggc gtctcagtcg gttccgcaat ggcgcctccg ctacatgcga
     6001 gcacgatgat gctcagggcc accaaggcca ggcgtctcac gcgacgagct ccttttcgcg
     6061 cggtgtgtgg aagcgcggca ccatccgcga atcacctggc cagcgatgcc gcgatgcaag
     6121 cccagggcgc cgctgcgccg tttcgatccg atacaccccg cgattgtcgc ggccataact
     6181 caggatgcgc aggcttgcaa cgccgtccga agcaggacaa ggtcatgtac ggggccggac
     6241 cacctgcccg gctgtcgcct tcgacgacgg ctcctatcat tgggctcacc cgcgaaaccg
     6301 cggtttgcca gccaccacca gaaggggacg acgccgtggc tctcgaacgc atgcccaggt
     6361 acgacgtctc aaacgacggt atgggcccgt acgcgatctt ctactgcgat cgggatggac
     6421 gggagtaccg cagtactccg aacatcgcga cgacggtcaa ggaaaacgtg accaggggcg
     6481 cgctgggcgg ttttctgcgt aacgtgccga tcctcggcga tcaggcggca aaccaggtgg
     6541 acaacgatcg ataccggacg gacatgtccg cagacgagct caacagcgcc tggaacgcga
     6601 ccaagcagta cttccgatcc tgcccgacat gcagccagat cgtgtgcgtc ccggacttcg
     6661 acgagccgac cggcttctgc aaggaagatt ccccgcgaag ggccgaggtg gaagccgcgc
     6721 aggcacagca gtctgccgcg gccctgaagg gattggccga cgtctttggg atcagtggcg
     6781 ccatcaaagg cggcatggcc gctgcccaag cgcagtcgga agcgggtatg gccacgtgtc
     6841 caaaggatgg caccaaggcc gcggctggaa ccggcttttg ccccaattgc ggcagcgtca
     6901 tgacgcagcc ggcacccgtt gcgcaggctt cgacctcggc ctgcccgagc tgcgggaccg
     6961 caatggccgc tggggtcaag ttctgcccga actgcggcac tccagcagcg cagcccaagg
     7021 cctgcgcgag ttgcggaacg gaaatggccg ccggcgtcaa gttctgcccg aactgcggta
     7081 cgcccgcgac ctgagccgcg cttcgggact acgggcgtcg acggctgtcc ttgtcgcgga
     7141 cttacccgga cctggagaaa ttcggcaaga cgaccgtaag ggctacggcg agcaacaggc
     7201 cgagcgccag agcctgcagc gggtaccaga acctctggac gaggattcgg aacgtgaggc
     7261 caccgctggc gacgagcggg ctgatcgttt cccggaggtc gaccgccagg tcacccatcc
     7321 gcacgcgtgg cagctctgag tcgtcgggga ccgcaggtag ctccttgtac ggcaccaacc
     7381 acggcgcgct ggtcacggcc cgatcgataa ccctcagcca catcacgacc tcgcgggcgg
     7441 cctgtccctc accatcgcga acggtccaga ggaggagaag cgccgctggc gtgaagatca
     7501 gcagagccgc aacaagaatg gtcccctggc caactggcgg acgcagcagc acgattagca
     7561 gggccggcac acagccgatg acaaggagtg tgatcgcgac gtagcgacga aagccacggc
     7621 tgcgcgcgta gtttgctagt cgctccagat cggggggcat gtcccgagat taacgccgac
     7681 cccgactcag ccggggatgg cgcttgggtc ttcccgacgc agaaggatgc ccaggtaacc
     7741 cttccaggga ccgacggtcg cgtcgtgagc cgcgctcatc cccgcgaaaa cctgtgaggt
     7801 gtgatccagg tcgtggacgg cccacgtcgc cagcaggttg cgcatggtca cgtcgccgag
     7861 gcccggatga gttccaacct tctccaggtc ggcgtcgccg acaagttcgt ccagctcgcg
     7921 caggctctgc gctcgcaact cgccgaagcg cttgagcagc gcgtccatgg gcacctctct
     7981 gtcgcgctcg agcatcgcgt ggcgatcgaa cttgtcgaat gcccgagtgg tggcgtagtc
     8041 caggatcatt cgaacgcgcg ggatccagtc gtccagctct gcggtgatca ggtgcccgac
     8101 gacgtcgcgc ggttgccagc cgtcaggcgt gtcccggcct tcgagccaga catccgggag
     8161 cccgccaagg agggccgaaa gcacacgggg cgttcgctgg agcagcgcct tcgtttggga
     8221 caggaattgc tcggccatga tgccgattat cgactgtcgc gagcgcgctg cttggcttcg
     8281 cgcagtcggg cgatcgtgtc tgtcggcgcc ggtgctgctg gcggttgtgt cggcgtcggc
     8341 gtcgcagtcg gagccgcggc cgtggccgca actggttgcg gtggtgcggc cgttttgatc
     8401 ggagcaggcc cggcctgcgg cctgaccggc ttcgggcccg tcaccgtcgc cgtcgccgtc
     8461 gtgtcggtct cggcgtccgg cgtccggaac aaggccgctc gtgcgctggc gccacccgcg
     8521 cgctccttgg ctgccagcat ttccccgacc tggcgcgttc gtcgagcagg tccacgccat
     8581 gaccgccagt gcgcaccagc ccaggcccgc gcgaggccga ggtcgccacg ctgcaaggag
     8641 acgcgtcgca cggcgacatc gagtggccac agcaccaggc ccagcaacag gagccacggc
     8701 accatgtcgg tcgcggcagt ggtcgtgccc aggtcgtgct cccacggctc ctcgggcgca
     8761 accagcgcct gcccgcccgt cgccgaacgg agagccgcga gcagccgctc attcgtgcca
     8821 agcaatcggt actcggccgg cggcggcgcg atgagcatca ccgtccgcgc cagcggcgtc
     8881 gttccgggct tcatctggtt gaaccgcaac gcgtacgcgc ccggcgtgag cgacccgagg
     8941 tcggcctcgt agacgcccgg cgccacctgg tcgagccgca gaccctcgag cagctcgaag
     9001 ttcgggtcgg tgatgttgac gatggtgtcg tagaagttac gtggtgtgcc gtcggactcg
     9061 acggaacgaa gccggagccg cgtgtcatcg ccctgggtga cgaactcggc ctcgatgccg
     9121 cccgactcct cgccggggaa cgtccacgac acgagctgcg agaagaactg gttgaagccc
     9181 gtccaggcca cccagttggt cgcccatcga ccctgggcat ccgacgtcca cgccacggac
     9241 cggccgaggc cgtactgcca ttgcgccagg acggggtcat cgcgggtgga cacgagaac
//
LOCUS       DATLCO010000095         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6014060, whole genome shotgun sequence.
ACCESSION   DATLCO010000095 DATLCO010000000
VERSION     DATLCO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6014060"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VMZ33_02390"
     CDS             complement(<1..1317)
                     /locus_tag="VMZ33_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUR16111.1"
                     /translation="MAHRATVGRLIARSLAAAGVEWAFTVPGESFLGVLDELPGVGIR
                     VVATRHEGGAAFMAEAVGQLTGTAAAVLGTRAVGAGNMAIGIHTARQNSTPMVAIVGQ
                     VQREFLGREAFQEVDQVESFGRLAKWAAEVDDPQTAADVVAAGLREMVDGRPGPILLS
                     LPEDVLDLPAPRRRAPSSHASQPSSAARLDRNAVREILQRLQAAKRPAILAGQGVIAA
                     DAVDQLVSLSERLSAPVFASWRRPTVFPNHHSNFLGMTGYGAADTVRPGLERSDFLLV
                     IGCRLNEITSFEYAVPGKRTRWAHVDLEPRTKHAGLGAPDIALAADAKMFLDVALDEL
                     ARAWEPPRDRSVALHRDRAAFLAASEVEQPDSFRGPGIHPGRVITTLEQVLPDDALLT
                     IDAGNFGGWVARGFRFGRRNGFLGPTSGAMGYGLPAAIAASLCQPRR"
     gene            complement(1419..1916)
                     /locus_tag="VMZ33_02395"
     CDS             complement(1419..1916)
                     /locus_tag="VMZ33_02395"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HUR16112.1"
                     /translation="MAGEVSFDVVSEFDRQELVNALDQARREMQTRYDFKGAKADLEL
                     GKDEIILRTDSESRAKAMRDLVESKAIRRNLSLKIFDWGEIEEAGGNTVRQRIGLRQG
                     LPDDLAKKITKMIRDDYAKVKTQIQGDAIRVSGKSKDDLQRVITRLREESEGYPVPLQ
                     FQNYR"
     gene            complement(1920..2339)
                     /locus_tag="VMZ33_02400"
     CDS             complement(1920..2339)
                     /locus_tag="VMZ33_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUR16113.1"
                     /translation="MATLTHGDAQDLVTAFKEAWEKRAPDLIVELFDDGADYRVDPFS
                     EPLSGINAIRDLWNNIAAAQTHVEFDAERVWLSGQTVLASWHAAYTRRATAERVRVRG
                     FMTMELGEGTPLRVMRFRQWPQERVVGMDSTFAAEEG"
BASE COUNT          409 a          856 c          799 g          425 t
ORIGIN      
        1 gcgccgaggc tggcacagcg aggcagcgat ggctgccggg aggccgtagc ccatggcacc
       61 cgacgtcggc ccgaggaagc cgttgcggcg accaaagcga aagccgcgcg cgacccagcc
      121 accgaagttg ccggcgtcga tggtcagcag cgcgtcgtcg ggcagaacct gctcgagggt
      181 cgtgatgacg cgtcctggat gtatgccggg tccgcggaag gagtcgggct gctcaacctc
      241 gctcgcggcg agaaaagccg cgcgatcccg atggagcgcc accgagcggt cgcgcggcgg
      301 ctcccaggcg cgtgcgagct cgtcgagtgc cacgtccagg aacatcttcg cgtcggcggc
      361 caacgcgatg tccggagcgc caagaccggc gtgtttcgtt cttggctcca aatcaacgtg
      421 tgcccagcgc gtgcgcttgc cggggacggc gtactcgaag ctcgttatct cgttgagccg
      481 gcacccgatc accagcagga agtccgatct ttcgagcccc gggcgaacgg tgtccgctgc
      541 gccgtagccg gtcatgccca ggaaattgga atggtggttg ggaaacacgg tgggtcgccg
      601 ccacgacgcg aatacgggtg cggacagcct ctcgctcaac gacacgagtt ggtcaactgc
      661 gtcggcggcg atcacccctt ggccagccag gatcgcgggc cgcttggccg cttgcaggcg
      721 ctggagaatt tcgcgcaccg catttcggtc gaggcgcgcc gccgacgatg gctgactcgc
      781 gtggctgctc ggggcgcgcc ttctgggtgc cggcaggtca agcacatctt ctggtagcga
      841 gaggagaatc gggcccggcc ggccgtcgac catctcgcgc aggcccgcgg caacgacgtc
      901 ggcggcagtc tgtgggtcat cgacctcggc cgcccacttt gccaggcgtc cgaacgattc
      961 gacctgatcc acctcctgga acgcctcacg ccccaggaac tcgcgttgga cctgccccac
     1021 gatggcgacc atcggggtcg agttctgccg tgccgtgtgg atgccaatgg ccatattgcc
     1081 cgcgcccacg gctcgcgtcc cgagcaccgc agcggccgta ccggtcagct gcccgacagc
     1141 ctcagccatg aatgccgcgc cgccctcgtg gcgtgtcgcg acgacgcgga tccccacgcc
     1201 tggaagctca tccagaacgc cgaggaagct ctcgcctggg acggtgaacg cccattcgac
     1261 ccccgccgcc gcgagcgacc gcgctatgag cctccctacg gtcgcgcggt gagccatcag
     1321 aaggcgtagc gcaccgagcc gaggatcagg ccgacgatca cgatgacagc gacgatggcc
     1381 atcaccaccc ggagcagcgg actgttcgag atcactgcct agcggtagtt ctggaactgc
     1441 agcgggaccg ggtagccttc cgactcctcg cgcaaccgcg tgatcaccct ctgcaggtcg
     1501 tccttgctct tgccgctcac gcggatggcg tcgccctgga tctgtgtctt gaccttggcg
     1561 tagtcgtcac gaatcatctt cgtgatcttt ttggcgaggt cgtcgggcag gccctggcgg
     1621 agtccgatgc gctgccgaac cgtgttgcca ccggcctcct cgatctcacc ccaatcgaag
     1681 atcttgagcg aaagattgcg ccggatcgcc ttggactcaa ccaggtcgcg catggccttg
     1741 gcccgagact ccgaatcggt acgcaggatg atctcgtcct tgcccagctc aaggtccgcc
     1801 tttgcgccct tgaagtcgta gcgcgtctgc atctcgcgac gcgcttgatc gagagcgttg
     1861 acgagctcct ggcgatcgaa ctcgctgacg acgtcgaatg agacctcacc ggccatatgt
     1921 cacccctcct ctgctgcgaa agtcgaatcc attccgacta cgcgttcttg cggccactgg
     1981 cgaaagcgca tgacccgtag cggcgtcccc tctccgagct ccatggtcat gaagccacgc
     2041 acgcggaccc gctccgcggt cgcccggcgg gtatatgccg cgtgccagct cgccaggacc
     2101 gtctggccac tgagccacac ccgctcggcg tcgaactcga cgtgggtctg cgcggccgcg
     2161 atgttgttcc agagatcccg aatcgcattg atccctgaga gcggctccga aaacgggtcg
     2221 actcggtagt ccgccccatc gtcaaacagc tcgacgatca ggtcgggcgc gcgtttctcc
     2281 cacgcctcct tgaacgccgt aacgaggtct tgtgcatcgc catgcgtgag cgtcgccatt
     2341 ccgcgcccag tctaggctgc tcgaccgtga cggcgaccgg atcgctcggc tcctcgtcgt
     2401 acacggtgat ccctggtgtg ccccgcgcag acggcgggta gacgctggca gccgcggcgc
     2461 gaccatatgc gtccgccagc gggtcgagg
//
LOCUS       DATLCO010000096         3634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6760754, whole genome shotgun sequence.
ACCESSION   DATLCO010000096 DATLCO010000000
VERSION     DATLCO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6760754"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VMZ33_02405"
     CDS             complement(<1..431)
                     /locus_tag="VMZ33_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HUR16114.1"
                     /translation="MTTQFSPTEADISMFRDVGYFVVPELFTPAECDAALGHIERAVF
                     ELALGDEKDGPLSYRPMMHLASPELTAVATDKRWAPIVLPLLGTGDARLYWEQAVAKP
                     PQARTELPWHQDNGYTPLIPQEYVTCWLALDDADEDNGCLWV"
     gene            complement(617..1069)
                     /locus_tag="VMZ33_02410"
     CDS             complement(617..1069)
                     /locus_tag="VMZ33_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR16115.1"
                     /translation="MIRGRDEGDATWFFNALMTTKASTAETAGAYSLTEHLVTAASNP
                     PMHVQFDEDEAFYILEGEVEFEVDGAVAVATPGTFAFVARGAAHLFRVRTDTARMLVI
                     CSGKPSHNLEEFFLGMGEPATARVLPQPGAPDEERLLALCAQTGIALV"
     gene            1171..1791
                     /locus_tag="VMZ33_02415"
     CDS             1171..1791
                     /locus_tag="VMZ33_02415"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HUR16116.1"
                     /translation="MTSPHAQPSTTTYAILGLLRLRPWTTYELAKQVQRSLGWFWPRA
                     ERKLYDEPKKMVAAGLADQRAEMTGARPRTVYSVTAEGRRALRRWLDEAPVPPTLEFE
                     GMIKVFFADGGSLTQLRKTLQSIAETAEARLAELDTKAAELARADTAFSDRAHLNALG
                     LRFHVDNERSVAAWARWAISQTAEWSSTTDPGSWDYRQALVRQHKK"
     gene            1843..2274
                     /locus_tag="VMZ33_02420"
     CDS             1843..2274
                     /locus_tag="VMZ33_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HUR16117.1"
                     /translation="MSASLQVLSMIDALNRGDIDAAIAYFDDAAENHGRRVGPEGMRA
                     VFEAQRIAFDDWHHEVVQTLVDGTTVATRSILSGVHRGTLPEPMVSLLFNGALRGIVP
                     TQRTVRIQAIHIWEVGSDGLIHAHWANRDDLGMRAQLSSGT"
     gene            complement(2304..2996)
                     /locus_tag="VMZ33_02425"
     CDS             complement(2304..2996)
                     /locus_tag="VMZ33_02425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR16118.1"
                     /translation="MRDIDTTETTRAPAAAISLRDATSEDAEFLARVMLIATRSHLGR
                     GAWDYLLGWEESATLEFLAQLAVSEPEHLFTYSRFVVATVDGEPAAAASGYRPERHGF
                     AVYLPVFDELLRTNGVTDPEYSQIWARGDVLQSAGAGPVAPGAWIIESVATLPAFRGM
                     GLVDRLLADLLQRGTASGCDRALIETVFVGNDPARRAYLKHGFLPVRETSGDGWQDIF
                     GSPGFEILERLL"
     gene            complement(3026..3436)
                     /locus_tag="VMZ33_02430"
     CDS             complement(3026..3436)
                     /locus_tag="VMZ33_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HUR16119.1"
                     /translation="MTDLLIDTSVSVPLLVASHAAHRSVLAAVGDRVIALAGHALNET
                     YSVLTRLPGDARLLPIDAIRLLRERFDQAAVLDARAARSAPGLLADHGLAGGATYDGL
                     IALAARSARLPLGTRDRRAEGTYRRVGVEVEMLY"
     gene            complement(3433..>3634)
                     /locus_tag="VMZ33_02435"
     CDS             complement(3433..>3634)
                     /locus_tag="VMZ33_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HUR16120.1"
                     /translation="LPKPLRDRLQLTPGTKVDVSEYGDGLHVSPVSRTARLEVRKGRL
                     VAVSDTKIDDDDVLGLIDAGRR"
BASE COUNT          682 a         1197 c         1140 g          615 t
ORIGIN      
        1 acccacaggc agccgttgtc ctcgtcggcg tcgtcgagtg ccaaccaaca ggtgacgtac
       61 tcctgcggga tcagcggcgt gtaaccgttg tcctgatgcc agggcagctc ggtgcgagct
      121 tgcggtggtt tggcgaccgc ctgctcccaa tacagccgtg cgtcgccggt gccgagcaat
      181 gggagcacga tcggcgccca gcgcttgtcc gtcgccactg cggttagttc ggggctggcg
      241 aggtgcatca tcggccggta cgacagcggc ccgtcctttt catcgccgag cgccagctcg
      301 aacaccgctc gctcgatgtg gccgagcgca gcgtcgcact ccgcgggcgt gaatagctcg
      361 ggaacgacga agtaacccac gtcccgaaac atcgagatgt ccgcttccgt cggtgagaat
      421 tgagtggtca tcgttcctcc agcgctagca cggtaccgat tctggtccca gttcccactt
      481 gcgcagttgg acatgtcttc gtcgtaggcc acgagcagcc catcgttgac gagctaccgc
      541 cacccgcaaa ggatcccgag aatcaactcg acctcgcgat gaatctctcg cgacgggttc
      601 accgcggccc ggacatctac accagcgcga tgcccgtctg ggcgcaaaga gcgagcagcc
      661 gctcctcgtc cggagcacca ggctgcggaa gcactcgtgc cgtcgccggt tcacccattc
      721 cgaggaagaa ctcctcgaga ttgtgcgacg gcttgccgga acagatcacg agcatccgcg
      781 cggtgtcggt gcggacacgg aacaagtgcg cggcgccacg tgcgacgaac gcgaatgtgc
      841 ccggcgttgc cacggcgacg gctccgtcga cttcgaattc gacttcgcct tccaggatgt
      901 agaacgcctc gtcctcgtcg aactgcacgt gcatcggcgg attggatgcc gccgtgacga
      961 ggtgctcggt gaggctgtag gcgccggccg tctcggccgt gctggccttc gtcgtcatga
     1021 gggcgttgaa gaaccacgtg gcgtcacctt cgtcgcggcc tcggatcatc gttgaagttt
     1081 gcatatcctc actcctccac tcgtatactg ccatctgcag tatatcgaat attagacact
     1141 gcccggggca gtatgtcaac ctcttgtgac atgacttctc cccacgcaca gccgtcaacc
     1201 acgacctacg cgatcctcgg attgctgagg cttcgtccgt ggactaccta cgagctcgcg
     1261 aaacaggtgc agcgatcact cggttggttc tggccgcggg ccgaacgaaa gctgtacgac
     1321 gagccgaaga aaatggtcgc agccggcctc gccgatcaac gggccgagat gaccggcgcc
     1381 cgacctcgca ccgtttacag cgtcacggcc gagggccgca gagcgctgcg ccgctggctc
     1441 gacgaggcgc ccgtcccgcc cacgctcgag ttcgagggaa tgatcaaggt cttctttgcc
     1501 gacggaggct cgctaacgca actgcgcaag acgttgcagt cgatcgccga gacggcggag
     1561 gcacgcctgg ccgaactcga taccaaagcc gccgaactcg cgcgcgccga caccgccttc
     1621 tccgatcggg ctcacctcaa cgcgcttggt ctgcggttcc acgtggacaa cgagcgctct
     1681 gttgccgcct gggcgcgctg ggccattagc cagacggcgg aatggagttc cactaccgac
     1741 cccgggtcgt gggactaccg gcaggcgctg gtaaggcagc acaaaaaata gagacaacgc
     1801 tgcaatagtt ggctctgact tcttaagaaa aggccacgcc tcatgagcgc ctcgctccag
     1861 gttctgtcaa tgatcgatgc gctcaaccgg ggcgacatcg acgccgccat cgcctacttc
     1921 gatgacgcgg cggagaacca cggccggcgt gttgggccag agggcatgcg cgcggtattc
     1981 gaggcccagc ggatcgcgtt cgacgactgg catcacgagg tcgtacaaac gctcgttgac
     2041 ggcaccaccg ttgcaacgag gtccatcctg tccggcgtcc accgcggaac gctgcctgag
     2101 ccgatggttt cgctcctctt caacggagca ctgcgcggga tcgtgccaac gcaacgaacc
     2161 gtgcgcatcc aagccatcca catctgggag gtcggtagcg atggcctgat tcacgcccac
     2221 tgggcgaacc gagacgacct cggcatgcgt gcgcaactct caagcggaac ttgagtgcgt
     2281 aaaaaaccgt gaagagacac tcctcacagc aatcgttcga ggatctcgaa acctggcgac
     2341 ccgaagatgt cctgccaccc gtcgccgctc gtctcgcgca ctgggaggaa gccgtgcttc
     2401 aggtatgcgc gccgagcggg gtcgtttccg acgaaaacgg tctctatcag agcccgatca
     2461 cacccggaag cagtcccacg ctggaggaga tcggccaaga gacgatcgac gaggcccatt
     2521 ccgcggaacg cgggcaaggt cgccacgctc tcgatgatcc acgctcccgg agccacgggc
     2581 ccggcgcccg cactctggag tacgtcgcct ctcgcccaga tctggctgta ctcgggatcg
     2641 gtgacgccgt tcgtgcgtag cagttcgtcg aacacgggga ggtagacggc gaatccgtgt
     2701 cgctcgggac ggtagcccga ggcagcggcc gcgggctcgc cgtcgaccgt cgcgacgacg
     2761 aaccgcgagt aagtgaagag gtgttctggt tcgctcaccg cgagttgcgc gaggaactcg
     2821 agcgttgcgg actcctccca gcccaggagg tagtcccatg cgccgcgccc caagtgcgac
     2881 cgcgtcgcga tgagcatgac ccgggccagg aactcggcat cctctgatgt cgcatcgcgc
     2941 aggctgatcg cagcggcagg cgctcgggtg gtctcggtcg tatcaatatc gcgcacgggt
     3001 cgcgtcctcc gatcggtcga catagtcagt acagcatctc gacctcgacg ccgacgcgtc
     3061 gataggttcc ctcggcgcgc cggtcccgag ttccgagcgg aagccgcgcc gaccgtgcgg
     3121 ccagcgcgat gaggccgtcg taggtggcac ctccggcaag gccgtggtca gccagtagac
     3181 ctggcgcaga tcgcgccgcg cgcgcatcga gcacggccgc ctgatcaaag cgctcccgga
     3241 ggaggcgaat ggcgtcaata ggcaacagcc gagcgtcgcc tggcaaacgc gtcaggaccg
     3301 agtaggtctc gttcagtgcg tggcccgcca acgcgatcac acggtcccca acggcagcga
     3361 gcaccgagcg gtgcgctgcg tgcgacgcga cgagcagcgg cacagacaca ctcgtgtcga
     3421 tcaacagatc ggtcaccgac ggccggcgtc gatgagcccg aggacatcgt cgtcgtcgat
     3481 tttggtgtcg gataccgcga cgagacgtcc tttgcgcact tcaagtctgg cggtgcgcga
     3541 cacgggcgac acgtgaaggc catcgccgta ctcactcacg tcaaccttcg taccgggagt
     3601 caactgcagc cgatcgcgca gcggcttcgg taac
//
LOCUS       DATLCO010000097         3885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6761049, whole genome shotgun sequence.
ACCESSION   DATLCO010000097 DATLCO010000000
VERSION     DATLCO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6761049"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VMZ33_02440"
     CDS             <1..702
                     /locus_tag="VMZ33_02440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUR16121.1"
                     /translation="DLKRYEYTEACCTLPEEYGFPEGSPLVGVVARLEPEKGHATLVE
                     AWPRVLEQVPEARLLIVGEGSQRQTLEARADALNLLGCGCAAPDHSVGTRHARPEAKV
                     LFTGLRDDVPAVTAALDVAVLPSYREAQGLAILEALALRRPVVATRVGGVPEMIEHRR
                     TGLLVPPRDPAALADAIATVLTDHPLADMIAKAGHDFVIANFSLDHMLAAVSAIYEEG
                     AAAISSRGTVSRTAA"
     gene            745..984
                     /locus_tag="VMZ33_02445"
     CDS             745..984
                     /locus_tag="VMZ33_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16122.1"
                     /translation="MNTVSITIRDVPRDARDEIAARAARSGRSLQEHVRLHLIEWANK
                     PSNDDILDRARERARATQTYLSAEEILGARDAGRR"
     gene            1408..2055
                     /locus_tag="VMZ33_02450"
     CDS             1408..2055
                     /locus_tag="VMZ33_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HUR16123.1"
                     /translation="MNNEYTTSGTISVSGIGCVAIEPDVAHVRLGVNISRKTVAEARA
                     EAATTMAAILAAVAEAGVPKHDVQSSFLSVQPRYDYRDGRAPELSGYDMNNVVTVTVR
                     DLAKLGDVIDGSLRAGATSMDGLEFRLADPAPAEREARIRAMANARSRADVIAEAADL
                     TISGVESVTEAGAMPPRPFAAKAERMMMSQDAATPVETGTMEIEVAVAVAYRTTA"
     gene            complement(2067..3143)
                     /locus_tag="VMZ33_02455"
     CDS             complement(2067..3143)
                     /locus_tag="VMZ33_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HUR16124.1"
                     /translation="MTHVFIAPHPDDIALSCGGLVASLRDLGQSVTILTVFSGSGQAA
                     ANGELSPYQRAALGFGSKVHWPLTEAFRRDNIAPDYVVPAAVGAGTPWQADPERIEIT
                     QERANTQARQFWQRAAWTRSANITNDETDARPVADSIGGQGSLAPIDYDALDVMERRK
                     VEDERFAYFSEASVIFLDLPDAVYRGYEGDEQLLGTVREDDPAPYDLIRSEILRLEPQ
                     MVYFPLGVGGHVDHQVCREIGLSLLAERRAWVMPGPSFVGRTTFYEDFPYSWWTDYDG
                     PNRPNGPSLDLPSGVSVEPQYADISDVIDRKAAGIQIYASQLDRLFESPQALLDDLYG
                     FHARTALAGGVNGLAERYWATVTP"
     gene            complement(3159..3833)
                     /locus_tag="VMZ33_02460"
     CDS             complement(3159..3833)
                     /locus_tag="VMZ33_02460"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HUR16125.1"
                     /translation="MRRWLRRSSGSDWIAEQLERRGVRDPDVLRAMRAVPREAFVPAR
                     SRNLSYSDGALPIGAGQTISQPYIVALMTEVLALSAWSAAHDGVRPRVLDVGTGSGYQ
                     AAVLIEIGADVLSIERQPELADRAREVLDRLGYRVEVRIGDGSTGAPDDAPFAGILVA
                     AASPQVPVPLVDQLLPDGRLVIPIGSRSEQVVTLVRRTDDGFVQEPIEAAVFVPLLGA
                     HGFADR"
BASE COUNT          682 a         1288 c         1273 g          642 t
ORIGIN      
        1 gatctcaagc gctacgagta caccgaggcg tgctgcacgc tgccggagga gtacggcttc
       61 cccgagggct cgccgctcgt cggcgtcgtg gccaggttgg aacctgagaa gggccacgcc
      121 acgctggtcg aggcctggcc gcgcgtcctg gagcaggttc ccgaagcgcg gctgttgatc
      181 gtcggcgagg ggtcgcaacg ccagacgttg gaggcacggg ccgacgcgtt gaaccttctg
      241 ggctgcggtt gcgccgcgcc cgatcactcg gttggcacac gtcacgcgcg gcctgaggcc
      301 aaggtgcttt tcacgggcct tcgcgacgac gtgcccgccg tcactgctgc cctggacgtg
      361 gccgtgctgc cttcctaccg cgaggcccag ggcctggcaa tccttgaggc gctggcgctg
      421 cgccggccgg tggtcgcgac tcgtgtcggg ggagtgccag agatgatcga acatcgccgc
      481 accggcttgc tcgtcccacc gcgcgacccg gcggcgctgg ccgacgccat cgccaccgtt
      541 ctgaccgatc accccctggc cgacatgatt gcgaaggccg gccacgactt cgtgatcgcg
      601 aacttttccc tggaccacat gctcgccgcg gtctcagcca tttacgagga gggggccgcc
      661 gccatttcct cgcgcggaac cgtatcgaga acggccgctt agttcacgct tgcactccgg
      721 ctgcattagc agcaaaatgc atgcatgaat actgtttcga tcacaatccg tgatgtgccc
      781 agggatgccc gcgacgagat cgctgcccgc gccgcacgaa gcgggcgctc gttacaggaa
      841 catgtgcgcc tgcacttgat cgagtgggca aacaagccct cgaacgacga catcctcgac
      901 cgagctcgcg aacgcgcccg cgcgactcag acctacctct ccgccgaaga gatcctcgga
      961 gctcgagatg caggccgtcg ctgacgaaac cgtcgtcgct gacgcgactg tcatcgtcgc
     1021 ggcgctagcg gacgccggga aggtcggtgc ctgggcgcaa aggtccctag tgggagtcac
     1081 cctcgccgca cctcacgtca tgccggttga agctgggtca gccctgcgcc gtcacgtctc
     1141 cgacggtcgt ctgtcagtag aaagtgcgac gttggcacat gggagcctcg ctgccatgtc
     1201 gttcgagtat ttcgcatacg aacctttcgc cactcgtatc tggcaattaa gagacaccgt
     1261 ctcaccgtac gatgcttggt atgtggcatt agccgaaatc cttgacgtgc cgatgctgac
     1321 gcttgatctg cgtttgacac gggcgtctgg accccgatgc aagtttcggg tgccgccttc
     1381 agaagacata atccaaacgg ggatggaatg aacaacgagt acacaaccag cgggacgatt
     1441 tcggtatccg gcatcggctg cgttgccatc gagccggacg tcgcccacgt gcgactcggc
     1501 gtcaacatct cgcggaagac cgtcgctgag gcacgcgccg aggcggctac gaccatggcc
     1561 gcgatcttgg ccgccgttgc ggaagccggc gtgccgaaac atgacgtcca gagctcgttc
     1621 ctctccgtcc agccgcgcta tgactatcgc gacggtcggg cgcccgaact gtcgggctac
     1681 gacatgaaca acgtcgtcac cgtcaccgtg cgcgatctgg ccaagctcgg cgacgtgatc
     1741 gacggctcac tgcgcgctgg agccaccagc atggacggtc tcgagttccg gttggccgac
     1801 ccagctccgg cagagcgcga ggcccgtatc cgcgcaatgg ccaacgcacg cagccgcgcc
     1861 gacgtgatcg cagaggccgc tgatctgacc atttccggcg tggagtcagt cacggaagct
     1921 ggagcaatgc cgccgcgccc gttcgcggcc aaggccgagc ggatgatgat gtcccaggac
     1981 gcggcaacgc cggtggaaac cggcaccatg gaaatcgagg tcgccgtcgc cgtcgcctac
     2041 cggacgacgg cctaggcgaa ccgcgcctag ggcgtgacgg tcgcccagta acgctctgcc
     2101 aggccgttca cgccgcccgc gagagccgtt cgtgcgtgga aaccgtacag gtcgtcgagc
     2161 aacgcctgcg gtgactcgaa cagccggtcg agctggctgg cgtagatctg gatgccggcg
     2221 gccttgcgat cgatcacgtc cgagatatcg gcgtattgcg gctccacgct gactcccgac
     2281 ggcaggtcga gcgacggacc attcggccgg ttgggcccgt cgtagtcggt ccaccacgaa
     2341 taggggaagt cctcatagaa cgtcgtccgg ccgacgaacg acggtccggg catgacccac
     2401 gcgcggcgct ccgccagcag cgacagcccg atttcgcggc acacctggtg gtccacatga
     2461 ccgcctacgc cgagcgggaa atagaccatc tgcggctcga gacgcaggat ctcagaacgg
     2521 atgaggtcgt acggcgccgg gtcgtcctcg cgcaccgtgc cgagcaactg ctcgtcgccc
     2581 tcatagccgc ggtacacggc gtcgggcaag tccaggaaga tcactgaggc ctctgagaag
     2641 taagcgaagc gctcatcctc gaccttgcgg cgctccatga cgtcgagggc gtcgtagtcg
     2701 atcggcgcga gagagccctg cccgccgatg gagtctgcaa cgggccgcgc gtcggtctca
     2761 tcgttggtga tgttggccga ccgcgtccac gccgctcgct gccagaactg gcgcgcctgg
     2821 gtgttggccc gctcttgggt gatctcaatc cgctcgggat cagcctgcca tggtgtccct
     2881 gcgccgacgg ccgccggaac gacgtaatcg ggcgcgatgt tgtcgcggcg gaacgcctca
     2941 gtcagtggcc agtgcacttt cgatccgaac ccgagggccg cacgctgata tggcgacagc
     3001 tcgccattgg ccgctgcttg gccactgcct gaaaacacgg tcaggatcgt tacgctctgc
     3061 ccaaggtcgc gaaggctcgc gacaagtccg ccacacgaga gcgcaatgtc gtccggatgg
     3121 ggcgcaatga agacgtgtgt catcggcggc cgagcatatc agcggtcggc gaacccatgc
     3181 gcgccgagga gcggaacgaa cacagctgcc tcgatgggtt cctggacgaa tccgtcgtcg
     3241 gtgcggcgaa caagcgtgac gacctgttcg gagcgtgagc cgatcgggat gaccaaccgg
     3301 ccgtcgggaa gcagctggtc gacgagcgga acgggcacct ggggcgaggc cgcggccacc
     3361 aggatgccgg cgaacggcgc gtcatccgga gcgccggtgc tgccatcgcc gattctcact
     3421 tcaacccgat agccgagccg atccagcacc tcacgcgccc gatcagccag ctcaggctgg
     3481 cgctcgatag agaggacatc tgctccgatt tctatcagta cggcggcctg gtagccggat
     3541 ccggtgccga cgtcgaggac gcgcggccgt acgccgtcgt gcgcggcact ccacgccgac
     3601 agcgccagaa cctcggtcat gagcgcaacg atgtagggct ggctgatggt ctggccggct
     3661 ccgatcggca aggcgccgtc gctatacgac aggttccgac ttctggcggg aacgaatgcc
     3721 tcgcgtggca cggcccgcat tgcccgcagg acgtccggat ctcgtacgcc gcgccgctcg
     3781 agctgttcgg cgatccagtc ggaaccgctc gaccgtcgca accagcggcg catggcgtta
     3841 cgtggctggc gaccccgtgg gagttacggt cccgccggca gacgg
//
LOCUS       DATLCO010000098         3529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2343492, whole genome shotgun sequence.
ACCESSION   DATLCO010000098 DATLCO010000000
VERSION     DATLCO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343492"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VMZ33_02465"
     CDS             complement(<1..660)
                     /locus_tag="VMZ33_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR16126.1"
                     /translation="MKLTIVGSAPAWTRRSGNSSSCYLVEHRSTRIVLDLGQGSFAQL
                     WQHTKPADVAAVVISHMHADHNVDLIPLRHWVKFENRGYGPALFTPSALRKRFGDFQA
                     DAKFLADFAGESLRPGTFPVGDMTIEARRVTHIPDSWAFRVSGASGPGLVYSGDCGDP
                     DDLLPLIRPGDTLLCEAGFGDKPEKVPIHLTAGQAAQAAATGGARRLVLTHIQDRTEG
                     DA"
     gene            complement(671..1321)
                     /locus_tag="VMZ33_02470"
     CDS             complement(671..1321)
                     /locus_tag="VMZ33_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUR16127.1"
                     /translation="MAEQPKDRWICLDVGETLIDETRVWTIWADILGITPFTFMAAMG
                     AILARGGQFREVFGFLGRDDWEASRPRFAAAYGGFQEGDLYPDAKPAIDSLRSHGYRV
                     AVVANQPAERNAELRFLGVRAEVMAMSEELGVHKPAAEFYLEALRLMGSPDPASVAYV
                     GDRLDNDVFPAATAGMRPVWIRRGPWAAITSDTPPAGTLIVHSLTEMVERIGEVWA"
     gene            complement(1321..2562)
                     /locus_tag="VMZ33_02475"
     CDS             complement(1321..2562)
                     /locus_tag="VMZ33_02475"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HUR16128.1"
                     /translation="MDRTRRSDAGPSTVRADPLRYLEDDLEELRAKNLYRPLRIMSGR
                     QAVHATVEGRPVISLSSNNYLGLTTHPRLVEAAERAVRELGVGTGAVRTIAGATDLIE
                     ELEQRLADFKHVEAVLTLQSGLTANSGTIPAVTAEGDLIISDELNHASIIDGVRLSKA
                     SRAVYPHSDVDGLENVLREARANGGPNGPYRSILVITDGVFSMDGDIAPLPGICEVAD
                     RYEAAVMVDDAHASGVLGRNGRGTIDHFDLHGRVDIQVGTLSKAIGVMGGYIAGRQHL
                     KDFLTQRCRTLLFSTSQPPAVAAACIAAIDVLEQEPERIERLWDNTRFFKKALADLGF
                     DTGISETPITPVMAGDESKAQALAAGLFELGVFATSVVFPTVALGKARVRTIVTSEHS
                     HDDLQTCIDAFEKVGRELWLI"
     gene            complement(2569..>3529)
                     /gene="tdh"
                     /locus_tag="VMZ33_02480"
     CDS             complement(2569..>3529)
                     /gene="tdh"
                     /locus_tag="VMZ33_02480"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HUR16129.1"
                     /translation="PGELLIKVLAASVCGTDVHIERWDPWAQENFAETPMTFGHEMAG
                     VVVARGTGVTRIGVGEAVAVETHIVDWTCYQCRTGRAHVCQNMKILGVHRPGSFAEYV
                     VIPEVNAWATEGISPEIAALQEPLGNAVHAAFVEDVAGESVAVLGCGPIGLMAIGVLK
                     MSGARKVFATDLNPERRAIAQQMGADLVLDGRDDVAARLREETDGNGVDVVLEMSGSA
                     AALHQGLAAVTNGGRVSLLGTHTVPATLDISQEIIFKGVRIYGITGRRLWDTWYRSTA
                     LLEEGLDLSPIISHRLPLAEYAKAFDLVASGHAGKIVLLPQEG"
BASE COUNT          591 a         1179 c         1168 g          591 t
ORIGIN      
        1 cgcatcgcct tcggtccggt cctggatgtg cgtcagcacc aagcgtcgtg cccctccagt
       61 cgcggccgct tgcgccgcct ggccggccgt caggtggatc ggaacctttt cgggtttgtc
      121 gccgaacccg gcttcgcaca ggagcgtgtc gccgggacgg atgagcggca agaggtcgtc
      181 ggggtcgccg cagtccccgc tatacacgag gccgggccca ctcgcgcccg acacccgaaa
      241 cgcccacgaa tctgggatgt gggtcacgcg tcgagcttcg atcgtcatgt cacccacggg
      301 gaacgttccg ggacggaggc tctcaccagc gaagtccgcg aggaacttgg cgtcggcctg
      361 gaagtcgccg aaacgcttgc gcagcgcgct cggcgtgaac agcgccggtc cgtagccgcg
      421 attctcgaac ttgacccagt ggcggagcgg gatcaggtcc acgttgtggt cggcgtgcat
      481 gtgactgatg acgaccgccg cgacgtcggc cggcttcgtg tgctgccaca gctgcgcgaa
      541 tgagccctgt cccaagtcaa ggacgatccg agtcgaacga tgctcgacga ggtagcagga
      601 cgacgaattg cccgagcgac gagtccaggc cggcgccgag ccaacgatgg tcagcttcac
      661 gcgccggttc ctacgcccag acctcgccga tgcgctcgac catctccgtc aacgagtgga
      721 cgatgagcgt ccccgccggc ggggtgtcgg acgtaatcgc cgcccatggc ccgcggcgga
      781 tccacaccgg gcgcatgccg gcggtcgcgg ccgggaacac gtcgttgtcc agccgatcgc
      841 cgacataggc caccgacgcc ggatctgggg agcccatgag gcgcaacgcc tcgaggtaaa
      901 actcggcagc cggcttgtgg acgccgagct cctcgctcat ggccatcacc tccgcgcgga
      961 caccgagaaa gcgcagctcg gcgtttcgct cggccggctg attggccacg acggctacgc
     1021 ggtagccgtg tgagcgcagc gaatcgatgg ctggcttggc atccggatag aggtcaccct
     1081 cctgaaaacc gccgtaagcg gccgcgaacc gcggacgaga cgcctcccaa tcgtcgcggc
     1141 ccaggaagcc aaaaacctcg cggaattggc cgccacgagc caggatcgcg cccatcgcgg
     1201 ccatgaacgt gaatggcgtg atgcccagga tgtcagccca gatcgtccat acgcgagtct
     1261 cgtcgatgag cgtctcgcca acgtcgaggc atatccatcg atcctttggt tgctccgcca
     1321 ctagatgagc cagagctcgc gccctacctt ctcgaaggcg tcgatgcagg tctgaaggtc
     1381 gtcgtgcgag tgctcgctgg tgacgatggt tcggacccgg gccttgccca acgcgaccgt
     1441 tgggaaaacg accgacgttg cgaagacgcc cagctcgaac agccccgcgg ccagggcctg
     1501 cgccttggac tcgtcgccgg ccatgaccgg cgtgatcggc gtttccgaaa ttcccgtgtc
     1561 gaacccgagg tcggccagag ccttcttgaa gaagcgcgtg ttgtcccaga gccgctcgat
     1621 gcgctctggt tcctgctcca aaacgtcgat cgcggctatg cacgccgcgg ccacggccgg
     1681 cggctgggac gtcgagaaga gcagcgtccg gcagcgctgg gtcaggaagt ccttgagatg
     1741 ctggcggccg gcgatgtacc cacccatgac cccgattgcc ttggagagag tgccgacctg
     1801 gatgtccacg cggccgtgaa gatcgaagtg gtcgatcgtt ccgcgtccgt tccgccccaa
     1861 aactccggat gcgtgggcgt cgtcgaccat gacggccgct tcgtaccgat cggccacttc
     1921 gcagatgccg gggagtggcg cgatgtcgcc gtccattgag aagacaccat cggtgatcac
     1981 gaggatggac cggtatggcc cgttcgggcc gccgttggcg cgcgcttcgc gaagcacgtt
     2041 ctccagccca tcgacgtccg agtgcggata gaccgcgcgc gacgccttgg agaggcgcac
     2101 gccatcgatg atcgaggcat ggttgagctc gtcggagatg atgaggtcgc cctcggcggt
     2161 gaccgccggg atcgtcccgc tgtttgcggt caggcccgac tggagggtca gaactgcctc
     2221 gacgtgcttg aagtcggcca gtcgctgctc gagctcctcg atgaggtcgg ttgcgccggc
     2281 gatcgtgcgc acagccccgg tgccaacccc cagctcgcga actgcccgct cagctgcctc
     2341 gacgagacgc gggtgagtag tcagccccag gtagttgttg gaggacagac tgatgactgg
     2401 ccgaccctcg accgtcgcgt gcacggcctg gcgcccgctc atgatgcgta gcggtcggta
     2461 aaggttcttt gcgcgcagct cctcgagatc gtcctcgagg tagcgcagcg ggtccgcgcg
     2521 gacggtcgac gggcccgcgt cgctccgtcg cgttcggtcc atcgcttgtc agccctcctg
     2581 cggcaagaga acgatcttgc cggcgtggcc cgacgcgacg aggtcgaatg ccttggcgta
     2641 ctcggccagc ggcaggcggt ggctgattat cgggctcagg tcgagtcctt cctcgagaag
     2701 ggcggtcgat cgataccagg tgtcccatag ccggcggcca gtgatgccgt agatgcgcac
     2761 gcccttgaag atgatttcct gcgagatgtc gagcgtcgcc ggcacagtgt gcgtgcccag
     2821 cagcgaaacg cggccgccgt tcgtgaccgc cgcgagaccc tggtggagag cggcggctga
     2881 gccggacatc tctagcacca cgtcgacgcc gttgccgtca gtctcctcac gcaggcgagc
     2941 cgccacgtcg tcgcggccat cgaggaccag gtcggcgccc atctgctgag cgatagcccg
     3001 acgttcgggg ttgaggtcag ttgcgaacac cttccgcgcg ccggacatct ttaacacccc
     3061 gatggccatg aggccgatcg gaccacagcc caggacggcg accgattcac cggcgacgtc
     3121 ctcgacgaat gccgcgtgaa cggcgttgcc caagggctcc tggagtgcgg caatctctgg
     3181 gctgatgccc tctgtcgccc aggcgttgac ctccgggatc acgacgtact ccgcaaacga
     3241 acccggccga tggacgccca ggatcttcat gttctggcac acgtgcgccc gcccggtacg
     3301 gcattggtag caagtccagt cgacgatgtg cgtctcgacc gcgacggcct cgcccacgcc
     3361 gatgcgcgtg acgcccgttc cccgcgcgac gacgaccccg gccatctcgt ggccgaacgt
     3421 catcggcgtc tcggcgaagt tttcctgtgc ccaggggtcc cagcgctcga tgtgaacgtc
     3481 tgtgccgcat acgctcgcgg cgagaacctt gatgagcagc tccccgggg
//
LOCUS       DATLCO010000099         7769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9044826, whole genome shotgun sequence.
ACCESSION   DATLCO010000099 DATLCO010000000
VERSION     DATLCO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9044826"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VMZ33_02485"
     CDS             <1..471
                     /locus_tag="VMZ33_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HUR16130.1"
                     /translation="VTIQAQILDLMRRLQRETGTAIILITHDLGVVAEMCDRVAVMYA
                     GEIIEQTDTRSLFRQPLHPYTKGLIGSVPILGQPKEELAVIPGMVPNLLSLPEGCRFA
                     PRCSAREEHNVTPAEAVHPELLATEADHTVRCWLYHSDERTRDWTPPRASEARA"
     gene            471..1556
                     /locus_tag="VMZ33_02490"
     CDS             471..1556
                     /locus_tag="VMZ33_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HUR16131.1"
                     /translation="MAVSSVGTKVREIEPRPIGSPLVEIRGLEKRFPVKGGVLQRTLA
                     EVRAVDGVDLTIEKGETLGLVGESGCGKTTVGRLLLRLIEPTAGTIAFDGTDITKLSG
                     DALKPFRRRMQIIFQDPYASLDPRAAIGDSIGDGLRIHGIGTSAERQKRVKRMMDLVG
                     LAPYQARRYPHEFSGGQRQRVGIARALIVEPDLVVCDEPVSALDVSIQAQVLNLLRDL
                     QGELGLTLLFIAHNMAVVEHISDRVAVMYLGRVVEETDRARAYADPRHPYTQALMSAI
                     PLPDPDARRQRIILRGDVPSPISIPSGCRFHTRCWLFDQLNQPEDCDTVDPALRPVAG
                     DDQHVAACHYAERSREVLDQSTKERVS"
     gene            complement(1626..2927)
                     /locus_tag="VMZ33_02495"
     CDS             complement(1626..2927)
                     /locus_tag="VMZ33_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16132.1"
                     /translation="MTAPTMTPSQPQTSDASASGVSAEGANLVDVQNLKVYFPIRSGL
                     VQRMIGSVKAVDDISFEVRKGETLGLVGESGCGKSTTGRAVIRLREPTSGSIFFDGTD
                     LAKIDKNKLRRMRRRMQIIFQDPYSSLDPRMTVGSIISEPIETHGLKKGRAKRERVAE
                     LLEMVGLDPRYVNRYPHEFSGGQRQRIGVARALAVEPEFIVCDEPISALDVSIQAQVL
                     NLLTELKNRLNLTYLFIAHDLSVVKHVSDRVAVMYLGKIVEIAPPDALYTGPAHPYTR
                     ALLSAVPVPDPVAERKRRRVILRGDVPSPVNPPSGCRFHTRCWLYERLGKPENCRTDE
                     PPLRVLEGDHRAACHYAEEAMKTDVGIAHVTELTVRRGTPAEALKIARKPDAAHLAAP
                     ITAGEADLVPTVIEEEPPAPSVPDSTGPDAGYKPDDPTRPE"
     gene            complement(2927..4027)
                     /locus_tag="VMZ33_02500"
     CDS             complement(2927..4027)
                     /locus_tag="VMZ33_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16133.1"
                     /translation="MSPTADQASVAPQATVEVTLEDRLASEAREREVKRAKAENLLEV
                     RNLSTHFFTQDGVVRAVDDVSFELGYGETLGLVGESGCGKSVTALSLTRLVPNPPGRI
                     VAGEILFEGVDLLKLSSDEIRRLRGKDIGFIFQDPLSSLNPTLTIGYQISESVRQHLG
                     LSARASKDRVIELLTKVGIPRPKDRLNDYPFQFSGGMRQRAMIAIALACDPKLLLADE
                     PTTALDVTIQAQILELINRLSDEFKTAVLLITHDLGIAAGMCDRINVMYAGRIVETGT
                     TDQLFDNPKMPYSWGLLDSLPRLDDERGGKLRTIEGLPPLLINLPDACRFNPRCSYTR
                     DLCREKEPALTEREEAHFARCHGTEPGGWIPT"
     gene            complement(4029..4976)
                     /locus_tag="VMZ33_02505"
     CDS             complement(4029..4976)
                     /locus_tag="VMZ33_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16134.1"
                     /translation="MTARTTTAVPGSVEARRSSGIWRDAMGRLFKNRLAVLGLLFVIF
                     LAVLGTIGPNFAPYRYDFQDTPALIENLFIPIPPLQHPTHYLGTDELGHDILSRIMDG
                     AQISLSVALVVQLVVLVIGLPLGAIAGWFGGRIDTYLMRFTDVMYAFPDLLLIILMSV
                     AFRDTAFGRLYSGLFLVFIAIGLTSWVTVARLVRGQLLSLKEMEFVEAAKALGVRDRN
                     IVMRHLLPNAIGPIIVAVTLGIPGAILAEATLAYLGVGVQAPRASWGSLIAAGQDQIR
                     FSPHLVYIPGIMIALALISFTFLGDGLRDALDPKLKGKQ"
     gene            complement(5003..5920)
                     /locus_tag="VMZ33_02510"
     CDS             complement(5003..5920)
                     /locus_tag="VMZ33_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16135.1"
                     /translation="MAGYIVRRVLWLIPVLFVVSVITFTLMHLVPGGPWSSDKAVAPG
                     VQAAINRSYHLNEPFWQQYGRWVFDFIRGDFGPSFKYADRSINQIIAEGAPATIQLGL
                     MAFGLAVIIGIPLGIIAALGHNRWPDYVATFVSMIGISTPSFVLAILLIVVFSVWLKW
                     LPPLGWRGPDTWVMPVVALAGYQIAQIARYTRASMLEVTRKDYIRTAHSKGLRERAVV
                     VRHMIRNALIPVITILGPILAFLVTGSFIIEAFFGIPGIGRYYVQGIVQRDYSLIMAM
                     TVIYAFAVAAMNVVVDVLYGYIDPRIRYS"
     gene            complement(6063..>7769)
                     /locus_tag="VMZ33_02515"
     CDS             complement(6063..>7769)
                     /locus_tag="VMZ33_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HUR16136.1"
                     /translation="GSDEISIINSIQRGLLYLDGELNVVPMMATAMPEVSEDGTVYTF
                     TLGDWSYSDGTPIVAGDFVRAAQHLADPRNAFDYGYEMCWVAGVNATLGVDFGCSDGE
                     TPYVDPAGCAETGSEEACAFDDATIDALLQEIGVTAPDEHTVVFTLTQPTSFWNNIVA
                     MWLLTPVPEGQTSWAEAADIKASGPFMVSEWTHNATMTLVPNPEWNGTQPTLQRIEMS
                     LAGDPAAAVAAFERGDLDQVGVPSSDVRRVLADPALAELASRRTNLSIEYYDFANCPD
                     KATCPTNAGVGEGARIPGGSPSQNANFRKALAQAVDLVELKEVGFAGLGEPAYSPTMP
                     GIPGFPTLTADNAPTSFDVTAALASMATALDELDVAQPDQAAIDLLTVEGTCDATCAA
                     TKARVQLLAPIKFGYNCDAGHDQRVLYLANVWRTNLGFSGQQMDIRCTDFGTFRTERR
                     AGNVYDIARNGWGADFPHPDNQNRDLFACGAGNNNSKYCNEEYDALLNQGATAGSYDE
                     ALPFYQQAETMLADDQPVLFLRYGEGVSLIQPWVSGLTITVSDSQNRGDRFPETIQIL
                     AH"
BASE COUNT         1425 a         2374 c         2602 g         1368 t
ORIGIN      
        1 gtcacgatcc aggcccagat cctggacctg atgcggcgcc tccagcggga gacaggcacg
       61 gccatcatcc tgatcaccca cgacctcggg gtggtcgcag agatgtgcga ccgcgtcgcc
      121 gtgatgtatg ccggcgagat catcgagcag accgacacgc gctcactctt tcgccagccg
      181 ctgcatccct acacgaaggg cttgatcggc tccgtgccca tcctcggcca acccaaggag
      241 gagctcgccg tcatcccggg catggtgccc aacctgctaa gcctccctga gggttgccgc
      301 ttcgccccgc gttgcagcgc ccgcgaggaa cacaacgtga cgccggcgga ggcggtccac
      361 cccgagctgc tggccaccga ggccgatcac acggtccgct gctggctcta ccactccgat
      421 gagaggacgc gtgactggac gccgccccgc gcatctgagg cccgagcctg atggccgtct
      481 cgtcggtggg aacgaaggtc cgcgagatcg agccgcgacc catcggctcg ccgctcgtcg
      541 agattcgcgg gctggagaaa cgcttccccg tcaagggcgg cgtcctgcaa cgaacactgg
      601 ctgaggttcg agcggtcgac ggcgttgacc tcaccatcga gaaaggcgag acccttgggc
      661 tcgtcggcga gtcaggctgc ggcaagacca cggttggccg actgctcctg cggctgatcg
      721 aaccgacggc cggcaccatc gcctttgatg ggaccgacat caccaagttg tcgggcgacg
      781 cccttaagcc attccgccgg cgaatgcaga tcatcttcca ggacccctac gcgtcgctcg
      841 acccgcgcgc ggccatcggc gacagcatcg gcgacgggct gcggatccac ggcatcggca
      901 cttccgccga gcgacagaag cgcgtcaagc ggatgatgga cctggtcgga ctcgcgccat
      961 accaggcgcg ccgttacccg cacgagttca gcggcgggca gcgccagcgt gtgggcattg
     1021 cccgcgcgct gatcgtcgag cccgacctgg tcgtctgcga cgagcccgtt agcgcgctcg
     1081 acgtgtccat ccaggcgcag gtgcttaacc tgctgcgtga cctacagggc gagctcgggc
     1141 tgacattgct gttcatcgcc cacaacatgg ccgtggttga gcacatcagc gaccgtgtcg
     1201 cggtgatgta cctcggccgc gtggttgaag agaccgaccg cgcgcgcgcc tacgccgacc
     1261 cacgacaccc gtacacgcaa gcgctcatgt cggccatccc gttgccagat ccggacgcgc
     1321 gacgccagcg catcatcctg cgcggcgacg taccgagtcc gatcagcatt ccctccggct
     1381 gccgattcca cactcgctgc tggcttttcg accagttgaa ccagcccgag gattgcgaca
     1441 cggttgaccc agctctgcgc ccagtcgccg gcgatgacca gcacgtcgcc gcgtgtcact
     1501 acgccgagcg cagccgcgag gtgctcgacc agagcacgaa ggaacgcgtc agctagtcac
     1561 tgacggccgg ctcgtcggag tgtttagagc tgaggcgcgc ggcgtccgac agccggaccc
     1621 gccttctact cgggcctggt gggatcgtcc ggcttgtacc cggcgtcagg cccggtggag
     1681 tcagggacag acggagcggg tggctcctct tcgatgactg tcgggaccag atcagcctcg
     1741 cctgcggtaa ttggagcagc gaggtgcgcg gcatccggct ttcgcgcgat cttcagagct
     1801 tcggcgggtg ttcctcgacg gaccgtcagc tctgttacgt gcgcgatccc gacgtcagtc
     1861 ttcatcgcct cttcagcgta gtgacacgcg gcgcgatggt cgccttcgag aactcgcagc
     1921 ggcggctcgt ccgtgcggca gttctccggc ttgccgaggc gctcgtagag ccaacatcgg
     1981 gtgtggaagc gacagcctga cggcgggttg accggtgagg gaacgtcgcc ccgaaggatg
     2041 accctgcgac gcttccgttc agccactggg tccggcactg gaacggctga gagcaatgcg
     2101 cgcgtgtagg gatgtgccgg accggtgtag agcgcgtcgg gcggtgcgat ctcgacgatc
     2161 ttacccaggt acatgaccgc cactcgatcg ctgacgtgtt ttacgaccga caggtcgtgg
     2221 gcgatgaaca ggtacgtcag gttgaggcgg ttcttgagct ccgtcaggag gttgaggacc
     2281 tgggcctgaa ttgacacgtc gagcgcggag atgggctcgt cgcaaacgat gaactcaggc
     2341 tcgactgcga gcgcgcgggc gacaccaatg cgttgccgct ggccgcctga gaactcgtgc
     2401 gggtagcggt tcacgtagcg tggatcgagg ccgaccatct cgaggagctc ggctacccgt
     2461 tcccgcttgg cgcggccctt cttgagtccg tgcgtctcaa ttggctcact gatgatcgag
     2521 ccgacggtca ttctcgggtc gagcgacgaa taagggtcct gaaagatgat ctgcatgcgc
     2581 cgtcgcatgc ggcgcagctt gttcttatca atcttcgcca ggtccgtgcc atcgaagaaa
     2641 atggatccgc tcgttggttc acgcagtcgg atcacagcgc ggccggtggt gctcttgcca
     2701 catccggatt caccgacgag gccaagtgtc tcgcccttgc ggacctcgaa gctgatgtcg
     2761 tccacggcct tcaccgaccc gatcatgcgc tgcacgaggc cggaacggat cgggaaatag
     2821 accttcaggt tctggacatc gacgaggttc gcgccttcgg cggagacgcc actcgcggac
     2881 gcgtcggacg tctggggctg cgagggagtc atggtgggcg ctgtcatcag gtcggaatcc
     2941 atcctccggg ttcagtgccg tggcagcgcg cgaagtgcgc ctcctcgcgc tcagtcaacg
     3001 ccggctcctt ctcacgacac aggtcgcgcg tgtaggagca gcgcgggtta aagcggcagg
     3061 catcaggtag gttgatgaga aggggcggca gcccctcgat cgtgcgtagt ttgccaccgc
     3121 gctcatcgtc gagtcgcggg agcgagtcga gcaggcccca cgaataaggc atctttggat
     3181 tgtcgaaaag ctggtctgtc gtgcccgttt cgacgatccg tccggcgtac atcacgttga
     3241 tccggtcgca cattccggcg gcaatgccaa ggtcgtgggt gatcagcagc acggccgtct
     3301 tgaactcgtc agagaggcgg ttgatgagct ccaggatctg cgcctggatg gtcacgtcga
     3361 gggccgtggt cggctcgtcg gcgagcaaca gcttggggtc gcacgccaga gcgattgcga
     3421 tcatcgcgcg ctgacgcatg ccaccagaga actggaaggg atagtcgttc agccggtcct
     3481 tcgggcgggg tatgccgact ttcgtcagca gctcgatcac acgatccttt gacgcccggg
     3541 ccgagaggcc caggtgctgc cggaccgact cggagatctg gtagccgatc gtgagcgttg
     3601 gattgaggct cgagagcggg tcctggaaga tgaaaccgat gtccttgccg cggagcctgc
     3661 ggatctcgtc gctcgagagc ttcagcagat cgaccccctc gaagaggatc tcgccggcaa
     3721 cgatccggcc cggcgggttg ggcacgagtc ttgtcaacga cagggccgtg acggacttgc
     3781 cacaacccga ctcgccaacg agaccgagcg tttcgccgta gccgagctcg aagctcacgt
     3841 catccacggc gcgaacgacg ccgtcctgtg tgaagaagtg tgtggagagg ttgcgtacct
     3901 caagcagatt ctctgccttg gcgcgcttta cctcgcgttc acgtgcttca gatgcgagtc
     3961 tgtcctcgag cgtaacttca accgtggctt gtggcgccac ggacgcctga tctgctgtcg
     4021 ggctcaacct actgcttgcc cttgagcttg ggatcgaggg cgtcgcgtag gccgtctccg
     4081 aggaaggtga agctgatgag ggctaaggca atcatgatgc ccgggatata caccaaatgc
     4141 ggcgaaaagc gaatttggtc ctgtccggcc gcgatgaggc tgccccacga cgcgcgcggc
     4201 gcctggacgc ccacacccag gtaggccagc gtggcctcgg cgaggatggc ccccggaatg
     4261 ccgagcgtca cggccacgat gatgggcccg atggcgttag gtaggaggtg gcgcatcacg
     4321 atgttcctat cccgtacgcc gagtgccttg gcagcctcga cgaactccat ctctttcagc
     4381 gagagcagct ggccgcgcac aagacgcgcg acggtgaccc acgacgtcag accgatggct
     4441 atgaagacga ggaacaggcc gctgtacaag cggccgaacg ccgtgtcgcg gaaagcgacc
     4501 gacatgagga tgatcagcag aaggtctggg aaggcgtaca tgacgtcggt gaagcgcatg
     4561 agataggtgt cgatgcggcc accgaaccat ccggcgatgg ctccaagcgg caggccgatc
     4621 accaggacaa cgagctggac gaccagcgcc accgacaggc tgatctgcgc cccgtccatg
     4681 atgcgcgaca ggatgtcgtg ccccagctcg tcagtgccca ggtagtgagt cgggtgctgc
     4741 aacgggggaa tggggatgaa caggttctcg atcagcgcgg gcgtgtcctg gaagtcatat
     4801 cggtacggcg cgaagttcgg cccgatagtc ccgagaacgg cgaggaagat cacgaacagc
     4861 aggccaagta cggccagccg gttcttgaac agccgtccca tggcatcgcg ccagatgccg
     4921 ctgctgcggc gcgcttccac cgagcccggg acggcagtag ttgtcctggc ggtcatctcg
     4981 taacgcctct cagttctgag gtttagctgt agcggatgcg cgggtcgatg tagccgtaca
     5041 ggacatcgac gacgacgttc atcgccgcca cggcgaaagc gtagatcacg gtcatggcca
     5101 tgatcaggct gtagtcgcgc tggacgatcc cttgaacgta gtagcgaccg attccgggga
     5161 tgccgaagaa tgcttcgatg atgaacgagc cggttaccag gaaggccagg atcgggccga
     5221 ggatggtgat caccgggatc agcgcgttgc ggatcatgtg ccgcaccacg actgcgcgct
     5281 cacgcaggcc ctttgagtgt gccgtgcgta tgtagtcctt gcgagtgacc tcgagcatcg
     5341 aggcgcgcgt gtaccgggcg atctgggcga tctggtagcc cgccagagcc acgactggca
     5401 tgacccaggt atccggtccc ctccagccta acggtggcaa ccacttcagc cagacgctga
     5461 acacgacgat caggaggatg gccaggacga agctgggggt tgagatgccg atcatggata
     5521 cgaaggtcgc gacatagtcg ggccacctgt tgtggccgag cgcggcgatg attccgagcg
     5581 ggataccaat gatcacggca agaccgaacg ccatcaagcc gagctggatg gttgcgggcg
     5641 cgccttcggc aatgatctgg ttgatgctcc ggtccgcgta cttgaacgat gggccgaaat
     5701 caccgcgaat gaagtcgaag acccagcggc cgtattgctg ccagaagggc tcgttcaggt
     5761 gataagagcg attgattgcc gcctggacgc cgggagcgac agccttgtcg ctcgaccatg
     5821 gcccaccggg cacgaggtgc atgagtgtga acgtgatgac cgacaccacg aacaacacgg
     5881 gaatgagcca gagaactcgt cggacgatgt agcccgccac tgtcctacct gctagctacg
     5941 gagatggggt ggagacgcca cattaggcgc cgattgtgcc atatacgcga gtgggccgat
     6001 ctgtgaagac cggcccacct cgcgcgaagt cgtgctgggc ggtgattagc cgcccagcgt
     6061 gactagtgag ccaggatctg gatcgtctcc gggaaacgat cgccgcggtt ctgcgagtca
     6121 gacaccgtga tggtgagacc cgagacccag ggctggatca ggctgacacc ttcgccgtag
     6181 cggaggaaca gcaccggctg gtcgtcagca agcatcgtct ctgcctgctg atagaacggt
     6241 agtgcctcgt cgtagctgcc cgcggtggca ccctggttga gcagggcgtc gtattcctcg
     6301 ttgcagtact tggagttgtt gttaccggcg ccgcaggcga acaggtcgcg gttctggtta
     6361 tccgggtgcg ggaagtccgc gccccaaccg ttacgcgcga tgtcgtagac attgcccgca
     6421 cgtcgctcgg tacggaaggt accgaagtcg gtgcagcgga tgtccatctg ctggccgctg
     6481 aagccaaggt tcgtgcgcca aacgttggcg aggtacagca cgcgctgatc gtggccggcg
     6541 tcacagttgt agccgaactt gatcggggcg agaagctgaa cacgagcctt ggtggctgcg
     6601 caggtcgcgt cgcaggtgcc ttcgacggtg agcaagtcga ttgcggcctg gtcaggctgg
     6661 gcaacgtcga gctcgtcgag tgcggtggcc atcgaggcca gtgccgcagt gacatcgaag
     6721 ctggtcgggg cgttgtccgc ggtcagcgtc gggaagccgg ggataccagg catcgtcggg
     6781 ctgtaagcgg gctcaccgag accggcgaag ccgacttcct tgagctcaac caggtcaacg
     6841 gcctgggcaa gagccttgcg gaagttcgcg ttctgcgacg ggctgccacc cgggatgcga
     6901 gcaccctcgc caacaccagc gttggtgggg caggttgcct tgtccgggca gttcgcgaag
     6961 tcgtagtact cgatcgagag gttggtccgg cggctcgcca gctcggccag ggccgggtcg
     7021 gcaagaacgc gacggacgtc gctcgaagga acaccgacct ggtcgaggtc accgcgctcg
     7081 aacgcggcaa cagcggcggc cgggtcacct gcgaggctca tctcgatgcg ctgcagggtt
     7141 ggctgcgtgc cgttccactc ggggttcggg acgagcgtca tggttgcgtt gtgggtccac
     7201 tcagagacca tgaacgggcc tgaggccttg atgtcagcgg cttccgccca tgacgtctgg
     7261 ccctccggca ccggcgtcag gagccacatc gcgacgatgt tgttccagaa gctggtcggc
     7321 tgggtgagtg tgaagacgac cgtgtgctcg tccggagccg tgacaccgat ctcctggaga
     7381 agggcatcga tggtggcatc gtcgaaggcg caagcctcct cagagccggt ctcggcacaa
     7441 cccgcgggat cgacgtacgg ggtctcgccg tcggagcagc cgaagtcaac gccgagggtg
     7501 gcgttaacgc cggcgaccca gcacatctcg tagccgtagt cgaaagcgtt gcgcgggtca
     7561 gcgagatgct gggctgcgcg aacgaagtcg ccggcgacga tgggcgtgcc gtcgctatat
     7621 gaccaatcac caagggtgaa ggtgtagacg gtgccgtcct cgctgacttc gggcatggcc
     7681 gtcgccatca tcgggacgac gttcagctcg ccatcgaggt acagaaggcc acgctggatc
     7741 gagttgatga tcgagatctc gtccgaacc
//
LOCUS       DATLCO010000100         4264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1606761, whole genome shotgun sequence.
ACCESSION   DATLCO010000100 DATLCO010000000
VERSION     DATLCO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606761"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VMZ33_02520"
     CDS             <1..351
                     /locus_tag="VMZ33_02520"
                     /inference="COORDINATES: protein motif:HMM:NF020742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16137.1"
                     /translation="VRRVDREKGTVDLAGLAAELPGRLADYQQLLFQRALDFRAANTH
                     YADSYDEFKAVLDKEGGFLMAPWCGATECEKQINSETSASIRVIPFDSPDEAGECMID
                     GRPSERRVLFARSY"
     gene            complement(376..1740)
                     /locus_tag="VMZ33_02525"
     CDS             complement(376..1740)
                     /locus_tag="VMZ33_02525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUR16138.1"
                     /translation="MAEYDFAIIGAGAAGEACAFEALRLGASVAIIERELFGGSCPFW
                     ACMPSKALLHAAGVHALGGDYAWPRASEFRDYMINRERRDWPDDTGHVNRLRDAGADV
                     IRGAAKVIGPGRVQVNGDDGRREVRGRFLIVAVGTHSAVPDLPGLDSIAGWSNREATS
                     TRDLPRSLLVLGGGPTGVEMAQVMARYGVPVSVVHPTDHLNHRDHPRSSEYVADGLRH
                     DGVTLRLGVRAERIRAGAGDDAAHRFELSDGSHVDAHEVLFAIGRTAPLKDLGLETLG
                     VSVDDEERVQPDDHLRIADDTYVIGDPAGPEMHTHLAHYEGEIVARMALGKDVTPDFR
                     AIPRAIYTDPEAAGVGLLLEQAKAGGLDALELTVDLATSAKGYVSESVGHATIVVDRG
                     SRTLVGTFIAGPGASEAIHMAVLAVKARTPVAVLADTITAFPTTSRALGGLFVEADRA
                     LARG"
     gene            1926..3350
                     /gene="nusA"
                     /locus_tag="VMZ33_02530"
     CDS             1926..3350
                     /gene="nusA"
                     /locus_tag="VMZ33_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HUR16139.1"
                     /translation="MNRDFIGALLQLNAEKNIPREQLVATLADAIQSAYRRATPGNEN
                     VYVDVDADSGRFKAYRAMQVVDEVEDPTIELTSDDAKKVDANARVGDLVKIADLDANS
                     FGRIHAQMATQLVRQRLREVEREQVFGQYAQRESEIITGTVSRVEPRGVILDVGRAEA
                     ILPTTDQSASEHYRIGQHVKAFVLEVRRTTKGPLIYVSRTHKNFLRRLFELEVPEIHA
                     GTVEVKAIAREAGSRSKVAVTSRQDGLDPVGATVGQRGGRVQAVVAELGGEKIDVIPW
                     NEDPAIFVANALSPAQVVSVTIDEDNRIANVTVPERMLSLAIGREGQNARLAAKLTGW
                     RIDIRSDQAPARQPAPAPDAEPSQTDETAAEVEPSAIEEVQEPATGFVRTPEGIDQAA
                     AVAVAVAEGEVVTEATVEAAAAGEDKPKRKRATKATPPVDTADAIAAETAPVEPAAAA
                     TETDGVKPKRAPRKKETTTVEAES"
     gene            3347..3652
                     /locus_tag="VMZ33_02535"
     CDS             3347..3652
                     /locus_tag="VMZ33_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HUR16140.1"
                     /translation="MTAPQTTDASQTAAPRRFPTRTCVACRAERQKREFIRIVRGPDG
                     TVAVDTTGRANGRGAYLCADASCWSSALKKKSIERALSVTLPAEVRAHLEGGVTHGA"
     gene            3642..>4264
                     /locus_tag="VMZ33_02540"
     CDS             3642..>4264
                     /locus_tag="VMZ33_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HUR16141.1"
                     /translation="MARSRSGTRNTRGPRKPPRRPDQNQSDSAVQTLEPGQRSAVDIP
                     PSITVKELAELLYVNAADIIRELIKSGIFANINQPLDRDTASLVAGELGFEVAEARAP
                     EKVEGNGEAPTPQAAKEVLFEEDDASLLLPRSPIVTVMGHVDHGKTSLLDAMRTTNVA
                     AGERGGITQHIGASEIIKDGKRTVFLDTPGHEAFTAMRARGARVTDIA"
BASE COUNT          781 a         1471 c         1389 g          623 t
ORIGIN      
        1 gtccgacgtg tcgatcggga gaaagggacg gtcgatctgg ccggtctggc tgccgagcta
       61 cccgggcgat tggccgacta ccaacagctc ctctttcagc gcgcactcga cttccgggcc
      121 gccaacaccc actacgccga ctcgtacgac gagttcaagg cggtcctcga caaggagggc
      181 ggcttcctga tggcgccgtg gtgcggcgcg accgagtgtg agaagcagat caacagcgag
      241 accagcgcct cgatccgcgt gatcccgttc gactcacccg acgaagccgg cgaatgcatg
      301 atcgacggcc ggccttcgga acgacgcgtc ctcttcgcgc ggtcctacta ggcgtccggt
      361 cagcaacacg tccgcttagc ctctggcaag ggcgcggtcg gcctccacga agagcccgcc
      421 cagcgcacgc gacgttgtgg ggaacgcagt gatcgtgtcg gccagcacgg ccaccggggt
      481 tcgtgccttc accgcgagca ctgccatgtg gatcgcctca gaggcgccgg ggccagcgat
      541 gaaggtaccg acgagcgtgc gcgagccacg gtcgacgacg atcgtggcgt gccccacaga
      601 ttcgctgaca tagcccttgg cgcttgtggc caagtccacg gtcagctcca acgcgtccag
      661 gccaccagcc tttgcctgtt cgaggagcaa gcccacgccc gctgcttcgg ggtcggtgta
      721 tatggcccgc gggatggcgc gaaaatcggg cgtgacgtcc ttcccgagcg ccatacgggc
      781 gacgatctcg ccctcgtaat gcgccaggtg agtgtgcatc tcggggccgg ccgggtcgcc
      841 gatgacgtat gtgtcgtccg cgatccggag gtggtcatcg ggttggacgc gttcctcgtc
      901 atccaccgaa acgccaaggg tctccaatcc cagatccttc agaggcgccg tccggccgat
      961 cgcgaaaagc acctcgtgcg cgtcgacgtg cgagccgtcc gacagctcga accggtgcgc
     1021 cgcgtcgtcg cccgcgccgg cgcggatacg ctccgcccga acgccgagcc gcagggtgac
     1081 gccgtcgtga cgcagcccgt cggcgacgta ctccgagctg cgcggatggt cgcggtggtt
     1141 gaggtggtcc gtgggatgta cgaccgagac cggcacgcca taacgcgcca ttacctgggc
     1201 catctcaacg cccgtcggcc caccgccaag gaccagcaac gaacgcggta gatccctcgt
     1261 tgacgtcgcc tccctgttcg accagccggc gatggagtcg agccccggca ggtcgggaac
     1321 agccgagtgc gtgcccacgg ccacgatcag gaacctgccg cgcacctcgc gcctgccgtc
     1381 gtctccgttg acctggacgc ggccgggccc tatgaccttc gccgcgccac ggatcacatc
     1441 tgcgccagcg tcccgcagcc gattcacgtg gccggtgtcg tcgggccagt cgcgccgctc
     1501 tcgattgatc atgtagtcgc ggaattctga ggcccgcggc caggcgtagt ccccgcccag
     1561 cgcgtgcacg ccggccgcgt gcagcagcgc cttggaaggc atgcacgccc agaatggaca
     1621 tgagccgccg aacagctcgc gctcgatgat cgcgactgac gcgcccaatc tgagcgcctc
     1681 aaaggcacag gcctcgcccg cagcgcccgc gccgatgatc gcgaaatcgt actcagccat
     1741 ggccgcgaac cctactggaa ggccgccgac tgatcaggcc ggaggggtta cccgtgtata
     1801 ctccgtcaga gtttctggcc gcctctgaac gtgggtgact cccacgttta tttatgcggt
     1861 gcgggatctg cggaggtaat ccctagcaat cgggggagcc cataggtcac tgaaaggagg
     1921 cgcatttgaa ccgcgatttc atcggcgcgc tcctgcagct gaatgctgag aagaacattc
     1981 ctcgcgaaca gctcgtggcc acgctcgccg acgcgattca gtccgcctac cgccgcgcga
     2041 ctccgggtaa tgagaacgtc tacgtcgacg tcgatgccga cagcggccgc ttcaaggcat
     2101 accgcgcgat gcaggtggtc gacgaggtcg aagacccgac catcgagctc acctccgacg
     2161 acgccaagaa ggtggatgcg aacgctcggg tgggcgacct ggtaaagatc gccgatctgg
     2221 acgcgaacag cttcggtcgc atccacgcgc agatggctac gcagctcgtc cggcagcgac
     2281 tacgcgaggt tgagcgcgag caggtgttcg gccagtacgc gcagcgcgag tccgagatca
     2341 tcaccggcac cgtctctcga gttgagccac gcggcgtgat cctggacgtc ggccgggcgg
     2401 aggcaattct cccgaccact gaccagagcg cctcggagca ctatcgcatc ggccagcacg
     2461 tcaaggcctt cgttctcgag gttcgccgaa cgaccaaggg cccgctcatc tacgtgagcc
     2521 gtacccacaa gaacttcctg cgccgccttt ttgagctcga agtgccagaa atccacgccg
     2581 gcacagttga ggtcaaggcg atcgcccgcg aggcagggtc gcgctccaag gttgccgtga
     2641 cttcacgcca ggatgggctc gatcccgttg gcgcgacagt cgggcagcgt ggcgggcgcg
     2701 tgcaggcggt tgtggccgag ctcggcggcg agaagatcga cgtcattccc tggaacgaag
     2761 acccggcgat tttcgtggcc aacgccctgt cgcccgcaca ggtcgtaagc gtcaccatcg
     2821 acgaggacaa ccgcatcgcc aacgtgacgg tgcctgagcg catgctctcc ctcgcaatcg
     2881 gccgcgaggg tcagaacgca cgcctcgccg ccaagctcac tggctggcgg attgacatcc
     2941 ggtccgacca ggcacccgcc cggcagccgg ctccagcgcc cgacgctgaa ccgagccaaa
     3001 ccgacgagac cgccgccgag gttgagccgt cagccattga agaggttcag gaaccggcga
     3061 caggcttcgt tcgcacgcct gagggcatcg accaggcagc cgcagtcgcg gtggccgtgg
     3121 ccgaaggcga ggtcgtgacc gaggcgaccg tcgaagctgc ggccgcaggc gaggacaagc
     3181 cgaagcgcaa gcgcgccacc aaggccacgc cgccggtcga caccgcggac gcgatcgcgg
     3241 ccgagaccgc acccgtcgag cccgccgccg cggcaacgga aacggacggc gtgaagccca
     3301 agcgagctcc gcgcaagaag gagacaacca cggtcgaggc ggaatcttga cggcgcccca
     3361 gacgacggac gctagtcaga ccgcggcgcc ccggcgcttt cccacgcgca cttgtgtcgc
     3421 gtgtcgagcg gagcgacaga agcgcgaatt catccggatc gtgcgcggcc cggacggaac
     3481 ggtggcagtc gacacgacgg gtcgtgccaa cggccgcggc gcgtacctgt gcgccgacgc
     3541 gtcctgctgg tcgtccgcgc tcaagaagaa gtcaatcgag cgcgccctgt cggtgaccct
     3601 gccggccgag gtgcgtgccc atctcgaagg gggagtaacg catggcgcgt agtcgcagcg
     3661 gcacccggaa cacacgtggc ccgcgcaagc cgccgcgccg tccggatcaa aaccagtccg
     3721 actctgccgt tcagacgctc gagccggggc agcgtagcgc ggtcgacatt ccgccctcga
     3781 tcaccgtcaa ggagctcgcc gagctgctct acgtcaacgc ggcggacatc atccgcgagc
     3841 tgatcaagag cggcatcttc gcgaacatca atcaaccgct cgaccgcgac accgcgagtc
     3901 tcgtcgccgg cgagctcggc ttcgaggttg ccgaggcacg cgcccctgag aaggtcgagg
     3961 gcaacggcga ggcgccgacc ccgcaggcag ccaaggaggt cctcttcgaa gaggacgacg
     4021 cgagcctgct cctgccgcga tcgccgatcg tcacggtcat gggccacgtc gaccacggca
     4081 agaccagcct gctcgacgct atgcgcacaa cgaacgtcgc cgcaggtgag cgtggcggta
     4141 tcacccagca catcggtgcc agcgagatca tcaaagacgg caagcgaacg gtcttcctcg
     4201 acacccccgg ccacgaggcc ttcacggcga tgcgcgcgcg cggcgctcga gtcaccgaca
     4261 tcgc
//
LOCUS       DATLCO010000101         2053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9051673, whole genome shotgun sequence.
ACCESSION   DATLCO010000101 DATLCO010000000
VERSION     DATLCO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9051673"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="VMZ33_02545"
     CDS             <1..1542
                     /locus_tag="VMZ33_02545"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HUR16142.1"
                     /translation="ADRRLNVVAVSDEPSVRALAISAGLPAYDTVANGEHALATFREQ
                     DRRLAERLGDVPPGESAAAQAAGEQSALQQSAIEQAVPWGSNAPPSRKRGDPLETDAS
                     ATPSVAVDSRGHRENSRRSRIGLGPILTAALVLLLLAGVGYGAYLFVPTASITLTPFT
                     TSLRPQSYSIVADPNVAVMDVVAGVVPAERLEIPLSVTTTFPATGIQAHETRAAGIIR
                     FRSENTLNSVAVAAGTIVATADGLEFETLEAANVPVAEFATSTPGTVDVPIRAIRPGT
                     RGNVAAGTITVVPALLGGQLVSAQNPDPTDGGRRIEERMISDLDYEAAVEVLHDQLES
                     SLAAKLQDPDTTPRGLTLFFPSAQTGDEVTTPSAVELVGTLGETFDLTVNSIATVTTV
                     NEALVDELAETRLREGLDPGVVLVNDAVQTTKSEGRVIVETVTYDVTAAASAYTEPDS
                     ASLLGAVRGKSIAAATEILSEYGMVEISMWPDFVDRLPDQAARISLAVVVPVATPQGS
                     PSPTP"
     gene            1539..1985
                     /gene="ruvX"
                     /locus_tag="VMZ33_02550"
     CDS             1539..1985
                     /gene="ruvX"
                     /locus_tag="VMZ33_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HUR16143.1"
                     /translation="MTRLLGVDVGERRIGLAVADIDTTSVRPLATIRRADPQRDGLTL
                     ARLAQEQRIDELIIGLPLNADGSEGAQAQLTRQWADSIAPYVARPIVWRDERLTSEAA
                     EQRIGMRSRGASGGPPSAAARNARRARIDREAAANIVQAEMDARKR"
BASE COUNT          365 a          700 c          677 g          311 t
ORIGIN      
        1 gccgatcggc gtctgaacgt ggtcgcggtg agcgacgagc cgtcggtgcg tgccctggca
       61 atctccgcgg ggctgccggc gtacgacacc gtggccaacg gcgaacatgc attggccacc
      121 ttccgggaac aggatcgtcg actcgcggag cgactcggcg acgttccccc gggcgaatcc
      181 gcggcggcac aggcagcggg ggagcaatcc gcgctccagc aatcggcgat tgagcaggct
      241 gttccgtggg gctcgaacgc accgcccagc cgcaaacggg gagatccgct ggagacggac
      301 gcgtcggcga cgccctccgt tgccgtggac tcgcggggtc atcgcgagaa cagccgacgt
      361 agcaggatcg gtctcggccc catcctgacc gccgctttgg tactccttct gctcgccggc
      421 gtgggctacg gcgcctacct gttcgtgccc acagcgagca tcactctcac gcccttcacg
      481 acctcgctgc gaccccagtc gtactcgatc gtggccgacc ccaacgtcgc cgtcatggat
      541 gtcgtggctg gcgtggttcc cgccgagcgt cttgagattc cgttgtcggt gacgacgaca
      601 ttccccgcga ccggcatcca ggcgcacgag acgcgcgccg caggcatcat ccgcttccgg
      661 agcgaaaaca cgctcaattc ggtggccgtg gctgcgggaa cgatcgtcgc gaccgcggac
      721 ggcctcgagt tcgagaccct cgaagcggcg aatgtgccgg tggcggaatt cgccacgagc
      781 actccgggaa ccgtcgacgt gcccatccgc gcaatccgcc ccgggacgcg cggcaacgtc
      841 gccgcgggga ccatcacggt tgtaccggct ttgctgggcg gccaactcgt cagtgcccag
      901 aaccccgatc ctactgacgg tggtcgccgg atcgaggaga ggatgatctc cgacctggac
      961 tacgaggccg ccgtcgaggt gctgcacgat cagctggaat cgtccctggc ggccaagctc
     1021 caggatcccg atacgacacc acgcggcttg acgctcttct ttcccagcgc tcagacagga
     1081 gacgaggtca ccacgccaag cgccgtggag ctggtcggga cgctcggcga aacgttcgac
     1141 ctgacggtga acagcattgc cacggtcacg accgtcaacg aggcgctggt cgacgagctg
     1201 gctgagactc gactccgcga aggtctggac ccaggcgtcg tgctggtcaa cgatgccgtc
     1261 cagacaacga agtcagaggg gcgcgtcatc gttgagaccg tcacctacga cgtcacggcc
     1321 gccgcctccg cgtacacgga gccagacagc gccagtctgc tcggagccgt acgcggcaag
     1381 tcaattgcgg ccgcaaccga gatactttcc gaatacggaa tggtggaaat ttcgatgtgg
     1441 ccggacttcg tcgaccggct tcccgaccag gcagctcgca tcagccttgc tgtcgtagtg
     1501 cccgtcgcca cgccccaagg ctcgccgagc ccgacgccgt gacgcggctc ttgggtgtgg
     1561 acgtcgggga gcggcgcatc ggtctggcgg tggccgacat cgacaccaca tcagttcggc
     1621 cgctagcgac gattcgccgc gccgacccgc aacgagacgg cttgacactg gcccggcttg
     1681 cgcaggagca gcgcatcgac gagctgatca tcggtctgcc gctcaacgcc gatggttccg
     1741 agggtgctca ggctcagctg acgcgccaat gggcggattc gatcgctccg tacgtggcgc
     1801 ggcccatcgt ctggcgcgac gaacgactta ccagcgaggc cgccgaacaa cgaattggca
     1861 tgcggtcacg cggcgccagc ggtggcccgc cgtcggccgc ggctcgaaac gccaggcgag
     1921 cccgcatcga ccgcgaggcc gctgcgaaca tcgtccaggc tgagatggac gctcgcaaac
     1981 gctgatgggg atcgaacgcg gacctcgcca ggaagcggcg cacaaggaac gcctggcagc
     2041 agcacaacaa gcg
//
LOCUS       DATLCO010000102         2608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4587319, whole genome shotgun sequence.
ACCESSION   DATLCO010000102 DATLCO010000000
VERSION     DATLCO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4587319"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VMZ33_02555"
     CDS             <1..375
                     /locus_tag="VMZ33_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16144.1"
                     /translation="DADAGLDSLAREMTASAGRIQTLQVTAAVRDARIGRHKVRRHDY
                     MVLGPDDGLVAVDTNRTRAVLAAIKKLKQGYELLTIYRGKELNVSTAEALRGAIAAEW
                     PDVEIEIVDGGQPHYDLLIAAE"
     gene            386..>2608
                     /locus_tag="VMZ33_02560"
     CDS             386..>2608
                     /locus_tag="VMZ33_02560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HUR16145.1"
                     /translation="MTAPSVDPHAADEGAATILDASLLDLKIVPPRVATLLRARLGLA
                     TVRDLLFHLPRRHDDFSRQMTMRDLRAKAIEGPVSATVTVVDLRVEQGFRRRIQRTVA
                     RISDDTGEGEAIWFGRRYIERRLNAGDEIMLSGKVELRGWVPRFQNPEFGPATPEALH
                     AGRIVPVYRLTAGVTGPYLRQRIRTALDMALDEYREYLPRDIRSLAGADAPSIGDAIE
                     WMHFPEDFGKLERALTRLAFDELLALQIGMVARQRQRRSERTDPIEVPDDDAKRAISG
                     VEQVISRQVSARRGATEQISLTDDQRNAVADIRSDLRSGRPMMRLIQGDVGSGKTAVA
                     ALALAAVADVGSQGALLAPTDLLARQHHVTLSALLEPLGHGVTLLTGSLGVAQRREAL
                     QLLGAPVAMATSGRSLGRVVVGTHALVQDAVAFEDLRLVVVDEQHRFGVAEREALTSK
                     GTAPHVLLMTATPIPRTLGQIVHADLDVSDLRTPPAGRQKIGTGIRSTDELVSRRDGS
                     GGALALMAAQVAAGYRAFVVVPLVEEDAESGARSVEQGAALVRDNWAAAAAIAGVDPA
                     VPDVEIVHGQMKASERDERMDRFRGGEVSIIVGTTVLEVGVDVPEATVMLILDADRFG
                     LAQLHQLRGRVGRGNAQSFCVLVTERYPHTRDDYNDPEKATVKARLDALVGTNDGFEL
                     AELDLEQRREGELLGLNQSGLPPLRVASLQRQLHRDLSLVARSAAEQLLDDTGRLGPE
                     N"
BASE COUNT          423 a          889 c          889 g          407 t
ORIGIN      
        1 gacgcggatg ccggcctcga ttccctcgct cgcgagatga cggcatccgc tggtcggatc
       61 cagacactgc aggtgacggc ggccgtacgc gacgcgcgca tcggccggca caaggtgcgt
      121 cgccacgact acatggtgct cggccccgac gacgggctcg tggccgtgga caccaaccga
      181 acgcgcgccg tcctcgcggc aatcaagaag ctcaagcagg gttacgagct gttgaccatc
      241 taccgcggca aggagctcaa cgtctcaacc gccgaagcgt tgcgtggcgc aatcgcagcc
      301 gagtggcccg acgtcgagat cgagatcgtc gacggcggcc agccgcacta cgacctcctg
      361 atcgccgccg agtgacacga gcggagtgac ggcgccgagc gtcgacccac acgccgcgga
      421 cgagggcgcg gccacgatcc tcgacgcgtc gctactcgac ctgaagatcg tcccaccccg
      481 cgtggcgacc ctgttgcgcg cacgcctcgg cctggcgacc gttcgcgatc ttctgttcca
      541 cctcccgcgt cggcatgacg acttcagccg ccagatgacc atgcgcgacc tccgtgcgaa
      601 agcgatcgaa ggccccgtct cggcgacggt caccgtggtc gacctgcgcg tcgaacaggg
      661 tttccggcgg cggatccagc gcaccgtggc gcgaatcagt gatgacacgg gggaggggga
      721 ggcgatctgg ttcggccgcc gctacatcga acggcgtctc aacgccggtg acgaaatcat
      781 gttgtcgggc aaggttgagc tgcgcggctg ggtcccacgc ttccagaacc ccgagttcgg
      841 cccggccacg cccgaagctc ttcacgcggg ccgcatcgtg cccgtgtatc gcctcaccgc
      901 cggcgtcacc ggcccgtacc tccgccagcg gatccgcacg gccctcgaca tggcgctcga
      961 tgaatatcgc gaatatctgc cgcgcgacat ccgatcgctg gccggtgccg atgcgccgtc
     1021 gatcggcgat gccatcgagt ggatgcactt cccggaagac ttcggcaagc tcgaacgggc
     1081 gctcacgcga ctggcgttcg atgagctgct cgccctccag atcggcatgg tcgcccgcca
     1141 acgccaacgc aggagtgagc ggaccgatcc tatcgaggtt cccgatgacg acgccaagcg
     1201 ggcgatctct ggcgtcgagc aggtcatcag ccgccaggtc agcgcacgcc gcggggcgac
     1261 agagcaaatc agcctgacgg acgaccagcg caacgccgtc gccgacattc gatcggacct
     1321 gcgctcaggg cggccgatga tgcggctcat ccagggcgac gttggctccg gtaagacggc
     1381 ggtagctgcg ctcgccctgg ccgcggtcgc cgatgtcggg agccagggcg cgctcctcgc
     1441 gccgacggac cttctggcgc gccagcatca cgtcacgctc tcggcccttc tggagccgct
     1501 cggccatggt gtgacgctcc tcaccggctc gttgggtgtt gcgcagcgcc gcgaggcgct
     1561 ccagctgctg ggtgcgccag ttgcgatggc aaccagcggc cggagcctcg gacgggtcgt
     1621 ggtgggcacg catgcgctcg tccaggacgc ggtagcgttc gaggacctgc gtcttgtcgt
     1681 tgtcgatgag cagcaccgct tcggcgtcgc cgagcgcgag gcgctgacga gcaaaggcac
     1741 ggccccgcac gtactgctga tgacagccac gcccatcccg cggactctcg gtcagatcgt
     1801 ccacgccgac ctggacgtct cggacctccg tacgccaccg gccggccgcc agaagatcgg
     1861 gaccgggatc cgatcgaccg acgagctggt atcgcggcga gacgggagtg ggggtgcact
     1921 ggcgttgatg gctgcccagg tagccgctgg ttatcgggca ttcgtggtcg tcccactcgt
     1981 cgaggaggac gcggaatcag gagcccgctc ggtcgagcag ggcgccgcgc tcgtccgcga
     2041 caactgggcc gcagctgccg ctatcgctgg cgtcgacccc gccgtgcccg atgtcgagat
     2101 cgtccacggt caaatgaagg cgtccgagcg cgatgagcgg atggatcgct tccgcggggg
     2161 cgaggtgtcg atcatcgtcg ggacgacggt gctcgaggtc ggtgtcgacg tgcctgaggc
     2221 gacggtcatg ctcatcctgg atgcggatcg gtttgggttg gcccagctgc accagttgcg
     2281 tgggcgtgtg ggtcgtggca atgcgcagtc gttttgcgtt ctcgtgacgg aacgctaccc
     2341 gcacacccgc gacgactaca acgaccccga aaaggccacg gtcaaagccc gccttgacgc
     2401 gctcgttgga acgaacgacg ggtttgagct ggccgagctc gacctcgagc agcgacgcga
     2461 gggcgagctc ctgggcctaa atcagagcgg cttgccgccg ctgcgtgtgg cgtcactgca
     2521 gcgccagctg caccgtgacc tgtcgctggt cgcgcggtcc gcggccgagc agcttctcga
     2581 cgacactggc cggctgggac ccgagaac
//
LOCUS       DATLCO010000103         3061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4588405, whole genome shotgun sequence.
ACCESSION   DATLCO010000103 DATLCO010000000
VERSION     DATLCO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4588405"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VMZ33_02565"
     CDS             complement(<1..786)
                     /locus_tag="VMZ33_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HUR16146.1"
                     /translation="MATPLFRALSVLLVALVVACASPVPTPTPERTDFPTLPTSTFYD
                     GPTPTSAAATETPSFTNPPTGTTSPTAEPTPAETTSTPTLPPDGSMAQILGTELGNTV
                     EAERVRLEVPGTSAVILFPDGSRWTGVFGLADVERDLPVQSDTTFAVGSVTKTFTTAA
                     IFQLHDEGALSIDDPLANWLPDYPNASAITLRHLLGHTSGVFNYFEDPQYNRRVFVTD
                     VGHRWTPDEILTAFAREPYGVPGEQFHYSNTGFVLLGLVVEQET"
     gene            836..>3061
                     /locus_tag="VMZ33_02570"
     CDS             836..>3061
                     /locus_tag="VMZ33_02570"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HUR16147.1"
                     /translation="MSIEPVSTPRARIAIAAARNGAEPRDFAASPVSRRSLNALERKL
                     EHELLVHHPGADTDTVRTAWQFAVEAHGPQTRVSGEPYVSHPLAVSRILAELGLDQDA
                     VVAALLHDIPEDTDYSIADVEERFGPEVSRLVDGVTKLGKLGARSHEQQQAENIRKML
                     LAMAQDLRVVLIKLADRLHNMRTIGVLPAEKQKRIAHQTMEIYVPLAERLGIWQIKWE
                     LEDLAFKVLEAAEFRHLASQLETRRKAREAYVRRAMAVLSDALADSKIKGDLSGRPKH
                     ISSIWKKMQRKGASLEEIYDIHAIRVLVDDVQDCYAALGVVHSIWRPIPGEFDDYIAV
                     PKPNGYQSLHTAVIALDGKPLEVQIRTHTMHRISEVGVAAHWGYKEGGKLDRDYNAKL
                     AWVRQLMDWQREVSDAEEFVEGVKLDLFQDQVFVFTPKGDVKDLPTGSTPLDFAYRIH
                     TDVGHSTIGAKVNNRLVPLDYKLKNGDIVEIVTTKAARGPSRDWLKSVHTGQAKEKIR
                     QWFKRQERDENIIHGREALDRELRRLARSSLEKLGAERVAEMSDLYKYPSVEDFFAAI
                     GYGAVSAQQVVTRMDVVDDANNVVLPQVAPPMPARTDGVRVKGVGDLMIRFARCCHPI
                     PGDPIIGYITRGRGVTIHLPTCLTVVNEKDIGRLIEVEWEGSAQQTYPIAIRIEAYDR
                     QGLLAEITNVVAENKVNIISANVRMNPDHTATLQATLQVASVTQLAKVLSRIEQVKDV
                     YS"
BASE COUNT          583 a          974 c         1006 g          498 t
ORIGIN      
        1 cgtctcctgc tcaaccacca ggccgagaag gacgaaacct gtgttgctgt agtggaactg
       61 ttcgccgggc acgccgtacg gctcacgggc gaacgcggtc aggatctcgt ctggcgtcca
      121 gcgatggccg acatccgtga cgaagacacg ccggttgtac tgcgggtcct cgaaatagtt
      181 gaagaccccg ctggtgtggc cgagcaggtg gcgcagcgtg atcgcgctcg cgtttggata
      241 gtcgggcagc caattcgcca gcggatcatc gatcgacagc gcgccctcgt catgtagctg
      301 gaagatcgcg gcggtggtaa aggtcttcgt cactgatccg acagcgaagg tcgtgtccga
      361 ctggacgggg aggtcgcgct cgacatccgc cagaccgaag acgccagtcc agcggctgcc
      421 atcgggaaac aggatcaccg cgctggtacc cggcacttcg aggcggacgc gctccgcctc
      481 caccgtgttg ccgagctccg tgcccaggat ctgggccatc gagccgtcgg gcggcagcgt
      541 cggggtcgaa gtggtctctg ccggagtcgg ctcggcagtc ggtgatgtag tccctgttgg
      601 cgggttggtg aatgagggtg tttcggtcgc tgcagccgat gtaggtgttg ggccgtcgta
      661 gaaggtcgag gtcggcagag tcgggaagtc ggtccgctct ggcgttggcg tgggcactgg
      721 tgaagcgcac gcaaccacga gcgcgacgag cagcacggac agggcgcgaa acaggggagt
      781 ggccatggtc tccacggtcc gaaaggaggg ggcctcacgg tacactcacc agcccatgtc
      841 gatcgagccg gtcagtacgc cacgcgcgcg catcgcgatt gccgcggcac gcaacggggc
      901 tgagccgcgc gactttgccg catcacctgt aagtcgacgg tcgctcaatg cgctggagcg
      961 caagctcgag catgagcttc tcgttcatca cccgggcgcc gacaccgaca cggtccgaac
     1021 cgcgtggcag ttcgccgtcg aggcccatgg cccccagaca cgcgtcagcg gcgaacccta
     1081 cgtcagccac ccgctggcgg tttcgcggat tctcgccgag ctcggccttg accaggacgc
     1141 ggtcgtcgcc gcgctgctgc acgacatccc cgaggacacc gactactcca tcgccgacgt
     1201 cgaggagcgc ttcggccccg aggtcagccg cctcgtcgac ggcgtaacga agctcggcaa
     1261 gcttggtgcg cgcagccacg agcagcagca ggccgagaac atccgaaaga tgctgctggc
     1321 gatggcgcag gacctgcggg tcgtgctcat caagctcgcc gaccgactgc acaacatgcg
     1381 cacgatcggc gtgttgccag ctgagaagca gaagcgcatc gcgcaccaga cgatggaaat
     1441 ctacgtgccg ctggcggagc ggctcgggat ctggcagatc aaatgggagc tcgaggatct
     1501 cgcgttcaag gtgctcgagg cggccgagtt tcgccacctg gcctcgcagc ttgagactcg
     1561 acgcaaggcg cgcgaggcgt acgtccgacg ggcgatggct gtgctcagcg acgccctggc
     1621 cgactccaag atcaagggcg atctttccgg ccgccccaag cacatcagca gcatctggaa
     1681 aaagatgcag cgcaagggcg cgtcgctcga ggagatctac gacatccacg ccatccgcgt
     1741 tctcgtggac gacgtccagg attgctacgc cgcgctgggc gtcgttcatt cgatctggcg
     1801 cccgatcccg ggggagttcg atgactacat cgccgtgccc aagccgaacg ggtatcaatc
     1861 gctgcacacg gcggtcattg cgctcgacgg aaagccgctg gaggtccaga tccggacgca
     1921 cacgatgcat cgcatctctg aggttggcgt ggccgcgcac tggggctaca aggaaggcgg
     1981 caaactcgac cgcgactaca acgccaagct cgcgtgggtc cgccagctca tggactggca
     2041 gcgcgaggtg tcggacgcgg aggagttcgt cgaaggcgta aagctcgacc tcttccagga
     2101 ccaggtcttc gtcttcacac cgaagggcga cgtcaaggac ctgccgaccg gatcaacccc
     2161 actggacttt gcctaccgga ttcacaccga cgtcggccac tcgacgattg gcgccaaggt
     2221 caacaaccgg cttgtgccac tcgactacaa gctgaagaac ggcgacattg tcgagatcgt
     2281 cacgaccaag gccgcgcgcg gcccgtcgcg cgattggctg aagagcgttc acaccggcca
     2341 ggccaaggaa aagatccggc aatggttcaa gcgccaggag cgcgacgaga acatcatcca
     2401 cggccgcgag gcgctggacc gtgagctccg ccgcctggcc cgctcgtcgc tggaaaagct
     2461 cggggccgaa cgcgtcgccg agatgtccga cctttacaaa tatccgtccg ttgaggattt
     2521 cttcgcggcg atcggctatg gcgcggtttc ggcccagcag gtcgttaccc ggatggacgt
     2581 tgtcgacgac gccaacaatg tcgtgctgcc gcaggtcgcg ccaccgatgc cggcacggac
     2641 cgacggcgtt cgcgtcaagg gcgtgggcga cctgatgatt cgcttcgcgc gctgctgcca
     2701 cccgattccg ggcgatccga tcatcggcta catcactcgc ggccgcggcg tgacgatcca
     2761 cctcccaacc tgtttgaccg ttgtcaacga gaaggacatc ggacggttga tcgaggttga
     2821 gtgggagggc tcagcccagc agacctatcc catcgcgata cggatcgagg cctacgaccg
     2881 gcagggcctc ctggcggaga tcaccaacgt ggtcgctgag aacaaggtca acatcatctc
     2941 cgccaacgtg cgcatgaacc ccgatcacac ggctacgctc caggcgacct tgcaggtcgc
     3001 gtcggtcacg cagctggcga aggtgctgtc gcgcatcgag caggtcaagg acgtgtacag
     3061 c
//
LOCUS       DATLCO010000104         2940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9056907, whole genome shotgun sequence.
ACCESSION   DATLCO010000104 DATLCO010000000
VERSION     DATLCO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9056907"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2295
                     /locus_tag="VMZ33_02575"
     CDS             <1..2295
                     /locus_tag="VMZ33_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUR16148.1"
                     /translation="VSEEEGTGIVHIAPGAGAEDYKLGKEIGLPVIAPLTEDGHYLAD
                     YGWLTGLDAQGVTERIVADLKERSFFYRLEPYTHRYPHCWRCGTALLFRVVDEWYISM
                     GEYYDKPRGEVTAEEKDRSLRYQIMDVVDQIRWIPGFGYERELDWLRTMSDWMISKKR
                     YWGLALPIWECADCGAFDVVGGRDELRQRAVSGWEEFEGHTPHRPYVDAVQVACKSCG
                     AATSRIKDVGNPWLDAGIVPFSTLHYRTEPDYWSNWFPADFITESFPGQFRNWFYSML
                     AMSTVLRNEPPFKTIFGYATLFAEDGRAMHKSWGNAIEFDEAAERMGVDVMRWMYAKH
                     KPEDNILFGYGTADAARRELLILWNVYAFFTTYARLAGWAPRRGIDEEIRAAGEWPIL
                     DRWILSRAAGLATDVGQELTDYDAMGATRLISTFIDDLSTWYLRRSRDRMRQTASEQD
                     RNTALATLHAALTSLARSVAPILPFLAEELYQNLMVGPVRAGSVPESVHLTEWPADAM
                     ADYRDEAVEGAMDTLRRAVELVRTLRGQQNIRLRQPLATMWLALPGGRLGDISAEDER
                     ALLDLLADEVNVRRVEVIGNESDLVERRVKPLLPMIGPRLGSQIPRVMAAARANEVEY
                     LPDGRVRLGGVELAPNEVEIIATPRPGTAVAHDEGVVAVIDTEIDDSLRAEGDAREIA
                     RAVQDLRKQAELELDDQIELWLSGPAQLIATLEPHIGQVARDTIASAVHREAAPSDLS
                     ASVVSLSGGDLTIAIRRVETANGR"
     gene            2285..2827
                     /gene="lspA"
                     /locus_tag="VMZ33_02580"
     CDS             2285..2827
                     /gene="lspA"
                     /locus_tag="VMZ33_02580"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HUR16149.1"
                     /translation="MADKGNRFRRWATFFALAGVVIVIDQVTKQYVDMNFPVASVHPL
                     PGLAEPTPVIDGIVRVAKTYNSGGIFGFFGDSAVVLALASTVVIALIVIYQWREGVRG
                     PWQLTVALGLLLGGALGNFIDRIRLQAVVDFVDMGIGDWRWYTFNVADAAISMALLVL
                     IVMSVFGDRFTPRVAAQPQA"
BASE COUNT          519 a          933 c          980 g          508 t
ORIGIN      
        1 gtgtccgagg aagaggggac gggcatcgtc cacatcgcgc cgggcgccgg cgccgaggac
       61 tacaagcttg gcaaggaaat cgggctgccg gtcatcgcgc cgctcacgga ggatgggcac
      121 tatctggcgg actacggctg gttgacgggc ctcgacgccc agggtgtgac cgagcgcatc
      181 gtcgccgacc taaaggagcg gagcttcttc taccgtcttg agccatacac gcaccgctac
      241 ccgcattgct ggcgctgcgg cacggcgctg ctattccgcg tcgtggatga gtggtacatc
      301 tccatgggcg agtactacga caaaccgcgc ggagaagtga cggccgagga gaaggaccgc
      361 agcctgcgct atcagatcat ggatgtcgtc gaccagattc ggtggatccc gggcttcggc
      421 tacgagcgcg agctcgactg gctgcgcacg atgtccgact ggatgatcag caagaagcgc
      481 tactggggtc tggcactgcc gatctgggag tgcgcggatt gtggtgcgtt cgatgtcgtc
      541 ggcggtcgcg acgagctgag gcagcgcgcc gtctcaggct gggaggagtt cgaaggccac
      601 acgccgcacc gcccgtacgt agacgccgtc caggttgcct gcaaatcgtg tggcgcggcg
      661 acgtcacgca tcaaggacgt cggcaaccca tggctcgatg caggcatcgt gcccttctcc
      721 acccttcact accggaccga gcccgactac tggagtaact ggttcccggc cgatttcatc
      781 accgagtcgt tccccggcca attccgcaat tggttctact cgatgcttgc aatgagcacg
      841 gtgctgcgga acgagccgcc gttcaagacg atcttcggtt acgccactct ttttgccgaa
      901 gacggacgcg cgatgcacaa gtcgtggggc aacgcgatcg agttcgacga agcagcggag
      961 cgcatgggcg tcgatgtcat gcgctggatg tacgccaagc acaagccgga ggacaacatc
     1021 cttttcggct acggcaccgc ggacgctgcc cggcgcgagc tgctcatcct ctggaacgtc
     1081 tacgccttct tcacgacgta cgcccggttg gccggctggg cgccgcggcg cgggatcgac
     1141 gaggagattc gggctgccgg ggagtggccc atcctcgacc gctggatcct gtcgcgtgcg
     1201 gcgggactgg ccacggacgt agggcaggag ctgaccgact acgacgcgat gggcgccacg
     1261 cggcttatca gcacgttcat cgacgacctc tcgacctggt atctgcgccg atcccgcgac
     1321 cggatgcgcc agacagcctc tgagcaagac cgaaacacgg ccctggcgac gcttcacgcg
     1381 gcgttgacga gcctcgcgcg ttcggtcgcg ccgatcctgc cgttccttgc agaggagctg
     1441 taccagaacc tcatggttgg gcctgtccgg gccggcagcg tgcccgagag cgtgcacctg
     1501 accgagtggc cggccgatgc catggccgac taccgcgacg aggccgtcga gggggcgatg
     1561 gatactctcc gtcgggcggt cgaactggtt cgtacgctcc gcggccagca gaacatccgt
     1621 cttcggcagc cactggccac catgtggctg gcactccccg gcggccggct gggtgacatc
     1681 tcagccgagg acgaacgagc tctgctggat ctgctcgccg acgaggtcaa cgtcaggaga
     1741 gtggaggtaa ttggaaacga gtctgacctg gtcgaacgcc gcgtgaagcc gctcctgccg
     1801 atgatcgggc cgcggctggg ctcgcagatc ccacgggtga tggccgcagc acgcgcgaac
     1861 gaggtggaat acttgcccga cggacgcgtc cgtttgggcg gtgtggagct cgcgccgaac
     1921 gaggtcgaga tcatcgccac gcctcgcccg ggcaccgccg tggctcacga cgagggcgtg
     1981 gtcgcggtca tcgacaccga gatcgacgac agtctgcgcg cagagggcga cgcccgcgag
     2041 atcgcccgcg ccgtgcagga tctgcgcaag caggccgagc tcgagcttga cgaccagatc
     2101 gagctgtggc tctcagggcc cgcgcaactc attgcaacgc ttgaacccca tatcgggcag
     2161 gttgcgcgcg acacaatcgc ttcggcagtt catcgcgagg cagcgccttc ggacctctct
     2221 gccagcgtcg tcagcctgtc gggcggcgac ctgacgatcg ccatccgtcg agtcgagacc
     2281 gcaaatggcc gataaaggga atcgtttcag gcgctgggca acattcttcg ccctggccgg
     2341 cgtggtgatc gttatagacc aggtcaccaa gcaatacgtc gacatgaact tcccggtcgc
     2401 ttcagtgcat cccctcccgg ggctcgccga gccgacgccg gtcatcgacg gcatcgtgcg
     2461 cgtcgcgaag acctacaaca gcggcggcat cttcggattc ttcggcgatt cagcggttgt
     2521 gctcgccctc gccagcacgg tggtgatcgc gctgatcgtg atctaccagt ggcgtgaagg
     2581 tgttcgcggt ccctggcagc tgacggttgc actcgggctg ctgctcggtg gcgcgctggg
     2641 caacttcatc gaccgcatca ggctccaggc ggtggtcgac ttcgtggaca tgggcattgg
     2701 cgactggcgc tggtacacct tcaatgttgc cgacgcggca atcagcatgg ctctgctggt
     2761 gctcatcgtg atgagtgtgt tcggcgatcg attcacccca cgggtcgcgg cacagccgca
     2821 ggcctagatg tcccaagcgc aaggaaggcg gcagagggag gccgtcaggc gcctctctga
     2881 caacgaccac gcaacagcgc ccgtgaggcc gactgcggag tccgaaacat tcacggtgac
//
LOCUS       DATLCO010000105         5077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3849248, whole genome shotgun sequence.
ACCESSION   DATLCO010000105 DATLCO010000000
VERSION     DATLCO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3849248"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VMZ33_02585"
     CDS             complement(<1..390)
                     /locus_tag="VMZ33_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16150.1"
                     /translation="MLIRLSAAVLAAALAAACGSTAPTTQPTAASNSQASTRPMASAQ
                     SEPTAQAEPSGQPEPTQGAVSSDGPTTQPPVEFSEVFPELTATDLNPAREPLPDPVFE
                     TADEIVEAMFTPGLEAPSVISMLEELGI"
     gene            667..1317
                     /locus_tag="VMZ33_02590"
     CDS             667..1317
                     /locus_tag="VMZ33_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HUR16151.1"
                     /translation="MPKINIDSFLTGLACTESSGRFDAVNQVTGAFGKYQVMPRNWPV
                     WAGRYLGNPWAPKSPRNQEFVVRQRIEQLHANHRTWRNVAHWWLTGNVHEDRTDWSPQ
                     ATRYVRRVMGFARAAAAPHRFGRVPKRCHPLDISPPRVRTDPFLRLRIAGGAVHVREA
                     PGAGNRVVAIAHRGQRVALLRLGLDAHAQRWVRVGLADGRSGWVASWYTERVEQGR"
     gene            complement(1326..2705)
                     /locus_tag="VMZ33_02595"
     CDS             complement(1326..2705)
                     /locus_tag="VMZ33_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16152.1"
                     /translation="MTVTPFVGVSAGRSENSSASQPWTWSLAVVRSLVLVPALLLALA
                     ATFVAPASPTAAGSAGSLDLRATYAVTAKLNWNQSSLFVSSVAHVINTRDSSVGQLVF
                     NALPTKLGRMRLIGASAGGHAAQAFVSGQSIIVTLPSAMGPGEERDVRIDYRALFNSD
                     TRGKKALFMKNNSIAAAYRWIPWLSREQQFTTPNFGESWVTGVSDRVDVTFMSDVPLT
                     YATSGWKTGGKGTTQSFTATGVRDFNFSASPSYRVTNVNWRGVDVQIFTRSTDPDRLW
                     YWTRLALDRFSDKIGVYPYRHFTVAETPAGVGMESPGMIWIDSTLEQSRFPYIVVHET
                     SHHWFFAVVGNNPATDPFVDEAMADFLTRDALASFRASSCGKTPLDKSVYEYSAACYA
                     EVVYVQGGAYIRDYRDEVGAQNFWDALSALYRERGFDIVGTRGFWTFLDERTGFNSAR
                     HADRFPSLF"
     gene            3077..3688
                     /locus_tag="VMZ33_02600"
     CDS             3077..3688
                     /locus_tag="VMZ33_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HUR16153.1"
                     /translation="MRIAPAMLAFIASVLFVFSSFFVAVPSVAASAGLNQCTPATTSQ
                     PPSTIAVYRVATGRVQRVDFKLYVARVTYHEWNVDATALRRAGAVAAKQYAWYHVLNW
                     RGGRAGGECFDVRDSTADQIYQAKSARSIPARIFKSVNDTWSWRMNRDGRFFMSGYRT
                     GTVAPCASDAGSKLYARSAKRCAKKGWSTERILRTYYTANVTK"
     gene            4110..4868
                     /locus_tag="VMZ33_02605"
     CDS             4110..4868
                     /locus_tag="VMZ33_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR16154.1"
                     /translation="MGRGRATAARVAARHAVDVADRLGAALRDARRKAGLTQADAARK
                     AGISQSLWSQLESARSTGWTLLTWDRAAHAVGASFEAFIRGASAADQPRDIAHLKAQE
                     LVIRTSRAGLWASLPEQNIDRDARSSRAADVLLHRHIPPRPPEYGLMEVFDWFPDVGD
                     PMRAWSSRLDAVERLAIARMGDDLPQVGGCWIVRATQRNRQLFKELASIFTARFPGDP
                     NAWLRALADPGSRMPKDAALLWVSVSGDRLFSRP"
     gene            complement(4878..>5077)
                     /locus_tag="VMZ33_02610"
     CDS             complement(4878..>5077)
                     /locus_tag="VMZ33_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16155.1"
                     /translation="SVAKRIRSYAGRDTEMRNLIASLDADAQQLAAPMPAAALKEMHF
                     RSYRSAKTRDEMGRAMRRPQS"
BASE COUNT          844 a         1610 c         1697 g          926 t
ORIGIN      
        1 gatcccgagc tcctccaaca tcgatatgac cgacggcgcc tccaggccgg gggtgaacat
       61 cgcttcgacg atttcatcgg ccgtctcgaa cacgggatcc gggagtggtt cgcgagccgg
      121 gttgaggtca gtcgcggtca gctccgggaa gacttcggaa aactcgaccg gtggttgagt
      181 cgtaggtcca tcgctggaga cggcgccttg ggtgggctcc ggctgaccgc tgggctctgc
      241 ctgtgcggtc ggctcggact gcgcactggc catcggccgt gtgctcgcct ggctgttcga
      301 agccgctgtc ggttgagtcg tgggcgcggt cgacccacaa gccgcggcaa gcgcggcggc
      361 aagaactgcg gccgagaggc ggatcagcat ggcgtgacga tggccgacac tacacccgcc
      421 gtcagccgtc ctgtcaactc tccatcgccc aaacaaacga gtcggcggcg cagtgcatca
      481 gccgatcccg caggggtatc cttgtggtgc gctctggtgc cctggatgcg cggcatgggc
      541 aaaagcccgc cagctgccgg acggccgtgt tcggtggaga cagggaagat gcgcgttcgt
      601 aggactgccg tgatctcggt cgcttgcctt gctttgctgc tgcccgcagc tgccaacgca
      661 ggtcctatgc cgaagatcaa tatcgactct tttttgactg gactcgcctg taccgagtcg
      721 agcggacgtt ttgacgcggt caatcaagtt accggcgcct ttggcaagta ccaggtcatg
      781 ccccgcaact ggccagtgtg ggcagggcgc tacctcggca acccctgggc gccgaagtcg
      841 ccgcgcaacc aggaattcgt cgtgcggcag cggatcgaac agctgcacgc caaccatcgg
      901 acgtggcgaa acgtcgcaca ttggtggctc accggcaatg tgcacgagga tcggacagat
      961 tggtcaccgc aagctacgcg ctacgtgcgt cgcgtgatgg gtttcgcacg cgcagcggcc
     1021 gcgccacatc gttttggtcg cgttccgaag cgctgtcatc cactcgacat atcacctccg
     1081 cgcgttcgca ccgacccttt cctccgtctg cgtatcgccg gtggcgcggt gcatgtccgt
     1141 gaggccccgg gtgcgggcaa ccgcgtggtg gcgatcgcgc accgaggtca gcgcgtcgct
     1201 ctgctgcgcc ttggccttga tgcgcacgcg cagaggtggg tgagagtcgg gctggccgat
     1261 ggccggtccg gttgggtcgc gtcctggtat acggagcgcg tggagcaggg tcgctaaccg
     1321 cgaccctaga acaggctggg gaaccggtcg gcgtgtctcg cgctgttgaa accggtgcgc
     1381 tcgtcgagga aggtccagaa gccgcgcgtg ccgacgatgt caaagccacg ctcgcggtac
     1441 agcgctgaca atgcgtccca gaagttctgg gcacctacct cgtcgcggta atcgcggatg
     1501 taagcgccgc cctggacgta gaccacctct gcgtagcacg cggcgctgta ctcgtagacg
     1561 ctcttgtcga gcggcgtctt gccgcagctc gacgcgcgga acgaggcgag cgcgtcgcgc
     1621 gtcaggaagt cggccatcgc ttcgtcgacg aacggatcag tcgcggggtt gttgccgacc
     1681 acggcaaaga accagtggtg gctcgtctcg tgcacgacga tgtaggggaa gcgcgattgc
     1741 tcgagtgtcg agtcgatcca gatcatcccc ggcgactcca tgcccacgcc ggccggcgtc
     1801 tcagcgaccg tgaaatgcct gtacgggtac acgccgatct tgtcagagaa ccggtcgagc
     1861 gccagccgcg tccagtacca gagccgatcg gggtcggtcg accgcgtgaa aatctgcacg
     1921 tctaccccgc gccaattgac gttcgtgacg cggtagctgg ggctggcgct gaagttgaag
     1981 tcacggacgc cggtggcggt gaagctttga gtggtgccct tgccacccgt cttccagccg
     2041 gacgttgcgt acgtcagggg cacgtcagac atgaacgtca catcgacgcg atcgctgacg
     2101 ccggtcaccc acgattcgcc gaagttcggc gtcgtgaact gctgttcgcg gctgagccag
     2161 gggatccagc ggtaggcggc ggcgatgctg ttgttcttca tgaacagcgc cttctttccg
     2221 cgcgtgtcgc tattgaagag ggccctgtag tcgatgcgga cgtcgcgctc ctcgcccggt
     2281 cccatggctg acggaagagt cacgatgatg ctctgaccgg aaacaaaagc ctgtgctgcg
     2341 tggccacctg cgctggcgcc aatgagtcgc atccggccga gcttcgtcgg cagggcattg
     2401 aacacgagct ggcccacgct cgagtcgcgt gtgttgatga catgcgccac gctggagacg
     2461 aacagcgacg actggttcca gttgagcttg gccgtgaccg cgtaggtggc gcgcaggtcg
     2521 aggctgccgg cggatcccgc cgcagtcggg gacgcaggcg cgacgaaggt agccgcgagg
     2581 gcaagcagca gcgcgggtac gagaaccaac gaccgaacaa cagccaacga ccaagtccac
     2641 ggctggctgg cggaactgtt ttcggatcgg ccggcactca ctcccacgaa aggagtgact
     2701 gtcatggggc acatcgtatc ggggccgcgc cggccctgat gtggtaactc tgccgtggtc
     2761 gattgggcct ttcgtctgcc tgagctccct gaagacggac ggcccgcgac ccgtcggtag
     2821 gtggcggcag acgccctttt gtcgcgtgcc tttaaggccg gtgaaatgat cgcggcgggt
     2881 tctgtattgg tcctgtcgtt ctcatttcgt tggtgttagg tagactgcgc cactgaaacg
     2941 agcggtctcc cgcgtgcgtc gccccagcga tagcgcctag agcggtgacg acccgaaagt
     3001 agttggttcg cctcgagcac cgtccccaga acggtgcgtg gagtcgtggc gaccagggaa
     3061 gagagtcata caagccgtgc gcatcgcccc agcgatgctg gcattcatag ccagtgtcct
     3121 tttcgtgttt tcgagtttct tcgtagccgt gccatctgtc gccgcgtcgg caggtctcaa
     3181 ccagtgcacc cccgctacaa cctcgcaacc gccctcaacg atcgcggtct accgcgtcgc
     3241 aacgggccga gtccagcggg ttgacttcaa gctctacgtg gcgcgcgtca cttatcacga
     3301 gtggaacgtc gacgcgacgg cactgcgtcg ggctggcgcg gtcgccgcga agcagtacgc
     3361 gtggtaccac gtgctcaatt ggcgcggtgg acgcgccggt ggggagtgct tcgacgtccg
     3421 tgactcaacc gccgaccaga tctaccaggc caagtcggcg aggtcgatcc ctgcgcggat
     3481 cttcaagagc gtgaatgaca cgtggtcgtg gcggatgaac cgcgacgggc ggttcttcat
     3541 gtcgggctat cggaccggga cagtcgcgcc gtgcgcctct gacgcgggtt cgaagctgta
     3601 tgcgcgatcg gcgaagcgct gcgccaagaa gggttggagc acggagcgga tcctgcggac
     3661 gtactacacg gcaaacgtca cgaagtaggg ctgccgcgag gccggccgtg gtcgccgccc
     3721 tcggcctcgg cccctccgag cagtcgagcc cggccaaata tcggccgtag tctcagaccg
     3781 acaggaaagc cgcctcggga acgtctcgga ggcggctttg ccgtttcgca ggcgtgccaa
     3841 gcgactcggt cacactgccg gcccgggcga acttcgactg aggacccacg ggcagccggc
     3901 ccgaatatca gtgccactgt tacaggtgtc gactgagtta gcggttctga ggttaaagcg
     3961 aggctttgag gcttcggtgt cgctgctacg tgagctttga ctgcgtgatt ggcgagttgc
     4021 gtgcggcccc aagcctcccc ggccctctac tgccctgtgg atgagcctgt aagaacgagg
     4081 taagcctcgc tcgtaacact gctgctgata tggggcgtgg gcgagcgact gcagcacggg
     4141 ttgctgcgcg acatgctgtc gacgttgccg accgtctcgg cgccgcgctg cgagacgcac
     4201 gccgcaaggc cgggttgact caggctgatg ctgcacgcaa agccgggatc agccagagcc
     4261 tgtggagcca actcgaatct gcccgctcca caggctggac gctactgact tgggatcgcg
     4321 cggcccatgc tgtcggcgcg agctttgagg cattcatccg cggcgcctcc gctgccgatc
     4381 agccgcgcga catcgctcac ctcaaggcac aggagctcgt aatcaggacg tcccgagctg
     4441 gcttgtgggc gagcctgccg gagcagaaca tcgaccgcga cgcgcggtcc tctcgcgctg
     4501 cggacgtgct tctccatcgt cacatcccgc cgcgccctcc cgagtacggg ctcatggagg
     4561 tcttcgattg gttccccgac gtcggcgacc cgatgcgcgc ttggtccagc cgccttgacg
     4621 cggtcgagcg cctggccatc gcacgtatgg gcgatgacct tccacaggtc ggcggctgct
     4681 ggatcgttcg cgccactcag cgaaatcggc agctcttcaa ggagctcgcg agcatcttca
     4741 cggcgcgctt tcctggcgac cccaacgcgt ggctccgcgc cctcgccgac cccggctccc
     4801 ggatgccaaa agacgctgcc ctgttgtggg tcagcgtctc cggcgatcgg ctattcagcc
     4861 ggccttgagg cccgcgatca ggactgcggg cgacgcatgg cgcgacccat ctcgtcgcgc
     4921 gtcttggccg accgatagct gcggaagtgc atctccttca acgcggccgc cggcatgggc
     4981 gcagcgagct gctgggcgtc ggcgtcgagc gacgcgatga ggttccgcat ctccgtgtcg
     5041 cggccggcgt acgaccggat ccgtttcgcg accgacg
//
LOCUS       DATLCO010000106         4352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9059305, whole genome shotgun sequence.
ACCESSION   DATLCO010000106 DATLCO010000000
VERSION     DATLCO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9059305"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VMZ33_02615"
     CDS             <1..257
                     /locus_tag="VMZ33_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUR16156.1"
                     /translation="GLEVELTENGWAGAIEYFFTQSGTRIHKTVHFFLMDPVGGSLDQ
                     HDTEFDEVRWVPLAEARRILSYPTERQIMEEALAAAGLEA"
     gene            254..1357
                     /gene="gcvT"
                     /locus_tag="VMZ33_02620"
     CDS             254..1357
                     /gene="gcvT"
                     /locus_tag="VMZ33_02620"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HUR16157.1"
                     /translation="MKQTALVERHRVLGARLIDFAGWEMPVQYGTGIIEEHRTVRQRV
                     GLFDLSHMGEVWVSGSGAGTGLASALVTDPPRLAVGRAHYSMITSSDGGIIDDLIVYR
                     VADERFMVVPNASNREEVARELRERMAGFDAAIDDASLRTSLVAVQGPRAAKLLQPLT
                     DVDLSALKYYAIVEGHACGQPAWVARTGYTGEDGFELFLDWDGAGPVWDVLTDKGQAF
                     GIAPCGLGSRDTLRLEAGMPLYGNELDRDTNPFEAGLGRVVKLDKPTDFVGRAALEAV
                     AQNGPQKQLVGLTIRGRGIARHGYTLHLPGDEAEVGVITSGAPSPSLGIPIAMGYLPP
                     SSTEVGTMVEVAIRDSKVEAEVVPLPFYKRAAS"
     gene            1382..1774
                     /gene="gcvH"
                     /locus_tag="VMZ33_02625"
     CDS             1382..1774
                     /gene="gcvH"
                     /locus_tag="VMZ33_02625"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HUR16158.1"
                     /translation="MQLPENLRYTRDHEWLRIEGDEAVVGITEYAASELGDIVFVELP
                     AAGTSLQSAQSFGVIESVKTASDLYAPTAGEVLTTNDALADKPELVNNDPYGEGWMIR
                     VRLADPGAMEGNELLMDADAYRELIAAG"
     gene            1796..3145
                     /gene="gcvPA"
                     /locus_tag="VMZ33_02630"
     CDS             1796..3145
                     /gene="gcvPA"
                     /locus_tag="VMZ33_02630"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HUR16159.1"
                     /translation="MTYGPHTTADREHMLGALGVGSIDALFADIPESVRARGLDLPPA
                     EDELTLARRLETLAARNKVGLASFLGAGAYRHHIPPTVDQILSRGEFYTSYTPYQPEI
                     SQGTLQTIYEYQSLIAELTGLDVVSASHYDGAAATAEAALMTVRATRRERVLVSRAVH
                     RHFRDTTATYFESGGLQIDELPTLPDGTTDLAALERDLTDPERPVAGVILGQPNAFGI
                     LEDMVEASRLAHAAGALFVAVVEPVSLAVLAPPGEYGADIAVGEGQPLGIALQYGGPY
                     LGLLAAKSDLVRQVPGRLVGRTTDIEGKRAYVMTLRAREQDIRRDKAASNICTNQALC
                     ALAATVYVATMGPHGLRDVAAGGAARAAELEQALADAGAPRVHSGTYLNEFAVRVANA
                     PNVHARLLEKGILAGLPLVHWYPDDPELHDALLVCATEVTTTDEIQHFAAALRELVA"
     gene            3142..>4352
                     /gene="gcvPB"
                     /locus_tag="VMZ33_02635"
     CDS             3142..>4352
                     /gene="gcvPB"
                     /locus_tag="VMZ33_02635"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HUR16160.1"
                     /translation="MTAVGPGVVTRPDDPAVAEAGQPEAVERGVARSRSPELGPQLAV
                     GPSLQPTLAELSRPGRGTTKVPHPPADALARIPTAQRRAGPLGLPELNEPEVIRHFVN
                     LSQLNFSVDTGFYPLGSCTMKYNPKINEWAARLPGFASLHPLAPDAAAQGTLELMWEL
                     ERWLVEISGMAAATLQPAAGAHGELTGILMIRAYHQSRTDTQRLEVIVPDSSHGTNPA
                     TASMAGFKTVTVPSNANGQIDLDALKGALSERTAAVMLTNPSTLGLFERGIKDVLDAV
                     HAAGALAYMDGANMNAVLGKFRPGQAGFDVMHFNTHKTFSTPHGGGGPGAGPVAVNEK
                     LAPFLPWPMVIRDGEGGDAGYRLEREGERPSSIGRVRSYQGSVGVLVRAYAYMLAHGG
                     EGLEQVSEDAVL"
BASE COUNT          734 a         1472 c         1458 g          688 t
ORIGIN      
        1 ctggcctcga ggtggagctc acggagaacg gttgggcggg cgcgatcgag tacttcttca
       61 cacagtcggg tacccgcatc cacaagactg tccacttctt cctgatggac ccagtcggcg
      121 gcagcctcga ccagcacgac acggaatttg atgaggtgcg ctgggtgccg ctggctgagg
      181 cgcgccgaat actcagctac ccgaccgagc gacagatcat ggaagaggca ctcgcggcgg
      241 caggcctcga ggcatgaagc aaaccgccct cgttgaacgc caccgcgtcc tgggcgcgcg
      301 tctcatcgac ttcgccggct gggagatgcc ggtccagtac ggaaccggga tcatcgagga
      361 gcatcggacg gttcgccagc gtgtcggcct gttcgacctg tcacacatgg gcgaggtgtg
      421 ggtcagcggc tcgggagccg gcacgggtct agcctcggca ctcgtgacgg acccgccgcg
      481 actcgcagtg ggccgcgctc actacagcat gatcacctcg tccgacggcg ggatcatcga
      541 cgacctcatc gtgtatcgcg tcgccgacga gcggttcatg gtcgtgccta acgcgtccaa
      601 tcgcgaggag gtcgcgcgcg agctgcgcga gcgcatggcg ggcttcgacg cagcgatcga
      661 cgatgcctcg ctgcgtacat ctctcgtcgc agtccagggg ccccgcgcgg cgaagctcct
      721 gcagccgctc acggacgtgg acctctcagc gctcaagtac tacgcgatcg tcgagggcca
      781 cgcctgcggt cagccagcct gggtcgcccg gaccggctac accggcgagg acgggtttga
      841 gctgtttctc gactgggatg gggctggtcc ggtgtgggac gttctcaccg acaaggggca
      901 ggcgttcggc atcgcaccgt gcggtcttgg ctcgcgggac actcttcggc tggaagcggg
      961 aatgccgctc tatggcaatg agctggatcg agacactaac ccgttcgagg ccggcctcgg
     1021 ccgcgtcgta aagctcgaca agccgaccga cttcgtcggc cgcgccgccc tcgaggcagt
     1081 tgcccagaac gggccgcaaa agcagctggt cggactgacg atccgtggcc gaggcatcgc
     1141 ccggcacggc tacacccttc acttgcccgg cgacgaggca gaagtcggcg tcatcaccag
     1201 cggcgcccca tcgccttctc tcggaatacc catcgcaatg ggctacctgc ctccgtcatc
     1261 gaccgaggtc ggtacgatgg tcgaggttgc catccgcgac tcgaaagtcg aggcggaagt
     1321 ggtcccgcta ccgttctaca agcgcgccgc ctcctagcgc ggcaccccag ggagaccaag
     1381 cgtgcagctg ccggagaacc ttcgctacac ccgcgatcac gagtggctcc gcatcgaggg
     1441 cgatgaggcg gtcgtgggca tcaccgagta tgccgcgagt gagctgggcg acatcgtgtt
     1501 cgtcgagctg ccggcagccg ggacgagcct ccagagcgct caatcattcg gcgtcatcga
     1561 atcggtcaag acagcgtccg acctatacgc cccgacggcg ggcgaagtcc tgacgacaaa
     1621 cgatgcgctg gccgacaagc cagagctggt caacaacgat ccctatggcg aaggctggat
     1681 gatccgtgtc aggcttgctg accccggggc gatggagggt aacgagctgc tcatggatgc
     1741 cgacgcttac cgcgagctga tcgcggccgg ctgattcccc caaccgactc ctgacatgac
     1801 gtacggaccg cacacaactg ccgaccgcga gcacatgctc ggcgctcttg gcgtcggctc
     1861 gatcgatgcc ctgtttgcgg atattccgga atctgtacgc gcgcgagggc ttgaccttcc
     1921 gcccgctgag gacgagctca cgctggcacg ccggctggaa acactggccg cacgcaacaa
     1981 ggttgggctg gcgagcttcc tgggcgcggg cgcgtaccgg caccatattc ccccgacagt
     2041 cgaccagatc ctgtcgcgcg gcgagttcta cacgtcgtac acgccctacc agcctgagat
     2101 cagtcagggc acgctgcaga ccatctatga gtaccagtcc ctcatcgccg agctgaccgg
     2161 cctcgacgtc gtctccgcgt cgcactacga cggcgccgcg gccacggcgg aggcagcgct
     2221 catgacggtg cgcgccacgc gtcgcgagcg ggtcctggtc agccgcgccg ttcatcgcca
     2281 cttccgtgac acgacagcga cgtacttcga aagcggcggc ctccaaatcg acgagctgcc
     2341 cacgctgccg gacggtacga ccgacctggc ggcgctggag cgcgatctga ccgaccccga
     2401 gcggcctgtg gcgggcgtga tcctcggcca accaaatgcc ttcgggatcc tcgaggacat
     2461 ggttgaggcg tcgcgccttg cgcacgcggc cggcgcactg ttcgtggccg tagtcgaacc
     2521 cgtctcgctc gcggtcctgg cgccgccggg cgagtatggg gccgacatcg cggtcgggga
     2581 gggccaaccg ctgggtatcg cccttcaata cggcggccca taccttggcc tgctggctgc
     2641 gaagtccgac ctggtccgcc aggtgcccgg tcggctggtc ggacggacga ccgacatcga
     2701 gggcaagcgg gcttacgtca tgacgctgcg cgcgcgcgag caggacatca ggcgcgacaa
     2761 ggcggccagc aacatctgta ccaaccaggc gctgtgtgcc ctggctgcga ccgtatacgt
     2821 ggccacgatg ggtccgcatg ggctgcgtga tgtcgccgcg ggtggggcgg cacgggccgc
     2881 ggagctggaa caggcgctcg cggacgccgg cgcgccgcgg gttcacagcg gtacttatct
     2941 caacgagttc gccgtgcggg tcgccaatgc gccgaatgtc cacgcgcggc tccttgaaaa
     3001 aggcatcctc gccggcttgc cgctcgtcca ttggtatccc gatgatcccg agctgcacga
     3061 cgcccttctg gtctgcgcca ccgaggtgac gacgaccgac gagatccagc acttcgcggc
     3121 cgcactccgg gagctcgtag cgtgaccgcc gtcggtcccg gagtcgtgac tcggccggac
     3181 gacccggccg tggccgaagc gggccagccc gaggccgttg aaaggggagt ggcgcgcagt
     3241 cgatcgcccg agctgggtcc gcagctcgcg gttggtccat cactccagcc gacgcttgcc
     3301 gagctgtcgc ggcccggccg cggcacgacc aaggttccgc atccgccggc agatgccctg
     3361 gcgcgcatcc ccacggccca gcgccgcgcc gggccgctcg gcctgcccga gctcaatgaa
     3421 cccgaggtca tccgccactt cgtcaacctg tcgcagctca acttttcggt tgacacaggg
     3481 ttctacccgc tcggttcgtg cacgatgaag tacaacccca agatcaacga gtgggccgcg
     3541 cggctgccag ggtttgcgtc actccacccg ctggcgccgg acgctgccgc gcaggggacc
     3601 ctcgagctca tgtgggagct ggaacggtgg ctggtcgaaa tcagcggcat ggccgccgcc
     3661 acgcttcaac cggcggcagg ggcacacggc gagctgacgg gcatcctgat gattcgggcg
     3721 taccaccagt cgcgcacaga cacccaacgg ctggaggtca tcgtccccga ctcttcccat
     3781 gggacgaacc cggccaccgc gtccatggcc ggtttcaaga cggtgacggt gccgtcaaac
     3841 gcgaatgggc agatcgacct cgacgcgctc aagggcgcgc tgtctgagcg aaccgcagcg
     3901 gtgatgctca ccaacccgtc gacgctcggc ctcttcgagc gcggcatcaa ggatgtgctc
     3961 gacgccgtcc atgccgcagg ggctctggcg tacatggacg gcgcgaacat gaacgcggtt
     4021 ctgggcaagt tcaggccggg ccaggccggc ttcgacgtca tgcacttcaa cacgcacaag
     4081 acgttttcga cgccgcacgg cggcggcggg cccggcgctg ggccggtcgc ggtaaacgag
     4141 aagctggcgc cgttcctgcc gtggccgatg gtgatccgcg acggcgaagg cggcgacgct
     4201 ggctaccggt tggagcgtga gggggagcga ccgtcatcga tcggccgtgt ccgctcctac
     4261 caggggagtg tcggcgtcct cgtgcgcgcc tacgcctaca tgcttgcgca cggcggcgaa
     4321 ggcctggagc aggtcagcga agacgccgtg ct
//
LOCUS       DATLCO010000107         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7514443, whole genome shotgun sequence.
ACCESSION   DATLCO010000107 DATLCO010000000
VERSION     DATLCO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7514443"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VMZ33_02640"
     CDS             complement(<1..1224)
                     /locus_tag="VMZ33_02640"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HUR16161.1"
                     /translation="MDIEVTRLDSDVAAVGREASVKQSSRPAPESGFAWRRAAITFLA
                     TLAVIVVIGGVAAITYERMHSGRILPGVSVAGVEVGGLPRAEAESRIRAVLPDVSAGQ
                     LTVRIGARTETISYSDIGRDYDMDAVLDSAFAVGRGAGLLEQLQTLTNGVAVPFQIRW
                     DHDQLTDRVKAMVATVESAPVDATLSRAGSSYSFTPSAEGGTVDEQAVYEQATAAVAD
                     TSRSAATIFIEPVPIAPSITTQQVQAAVDGFEATVASPLTITAGTQTVTVPSSVLSGW
                     VRLEATPVAGEWTVVIESAPITQIVDELAFDVDVAAVNASYTFERNTPTVIAAVDGLS
                     LDTSAAVAAITESLYSRSTGGPGTPLRFSLAAVPAEFTTEDAAALAGRVERLGRWVTH
                     YRPSPFNGDGKNIRNP"
     gene            complement(1311..>2070)
                     /locus_tag="VMZ33_02645"
     CDS             complement(1311..>2070)
                     /locus_tag="VMZ33_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUR16162.1"
                     /translation="GPTVRPSPSITAEPTPIDNSVGAAAVVVPLRSAELATRISGVVA
                     SIYVHNGSPVEDGQLLLKLEQKTYQDAIDVAQSRVDQAGRAVELARLQVEQLPLDASP
                     GQIEFVQASLRLAEADLDLAESRLREAQTALTQTEIRAPFAGTVASVDIELGEQALAG
                     QAVITVGDQSGWLIETTDLSELEVVRIAVGDRATVALDALPELVVNGTVERIQVRGTN
                     DDGGVVFAVSIRPDIHHSELRWGLTATVRIAPSG"
BASE COUNT          350 a          664 c          699 g          357 t
ORIGIN      
        1 gggattgcgg atgttcttgc cgtcaccgtt gaatgggctg ggccggtagt gggtgaccca
       61 acgaccgagc cgctccacgc gaccggcgag ggctgcggca tcttcggtcg tgaactccgc
      121 cggcacggcc gccagcgaga atcggagcgg agtgcccggg ccaccggtgc tgcgggagta
      181 cagcgattcg gtgatcgctg cgacggcggc gctcgtatcg aggctcagtc cgtcgacggc
      241 cgcgatgaca gttggtgtgt tgcgctcgaa cgtgtagctc gcgttcacgg ccgcgacgtc
      301 cacgtcgaat gccagttcat caacaatctg ggtgatcggc gcactctcga ttacaaccgt
      361 ccattcaccg gcgacggggg tcgcctcaag ccggacccag ccactaagaa cgctcgaggg
      421 caccgtgacg gtctgtgtgc cggcggtgat ggtcaggggc gaggcaacgg ttgcctcgaa
      481 cccgtcgacg gcagcttgaa cctgctgggt ggtgatcgag ggggcgatgg gtaccggttc
      541 aataaaaatc gtcgcggccg agcggctcgt gtcagcaacc gccgccgtcg cttgctcgta
      601 gacggcttgt tcatcgactg tgccaccttc agctgacggc gtgaaggaat acgaggagcc
      661 ggcgcggctc agggtggcat cgacgggagc gctctcgaca gtcgccacca tggccttgac
      721 gcgatcggtc agctgatcgt ggtcccacct gatctggaag ggcactgcca cgccattggt
      781 gagcgtctgg agctgctcga gcagccctgc gccgcggccg acggcgaagg cggagtcgag
      841 cacagcgtcc atgtcgtagt cgcgcccaat gtcggagtag ctgatggttt cggtgcgagc
      901 accgatcctg accgtcagct ggccagcaga gacatccggc aagacggccc gaatgcggct
      961 ctcagcctcg gcgcgcggca ggccgccgac ctcgacgccc gcgactgaaa cgccgggcag
     1021 gatccggccg ctgtgcatcc gttcatacgt gatcgcggca acgccgccga tgacaacgat
     1081 gacggccagc gtcgcgagga acgtgatcgc ggcgcgacgc caggcaaagc cagactcggg
     1141 agctgggcgc gacgattgtt tgaccgatgc ttctcgtccg accgccgcaa cgtccgaatc
     1201 gaggcgcgta acctctatat ccactgtcat atggtgacgg aagtcatgca gaaacggcaa
     1261 ttacgtggac acagtggcac gttgcggtcg cgcactacgc gacccgagtt tcagccgctc
     1321 ggtgcgattc tcactgtcgc cgtgagcccc cagcgcagct ctgagtgatg gatatcgggg
     1381 cggatcgata ccgcaaagac cacgccgcca tcgtcgttcg tgccacgcac ctgaatgcgt
     1441 tccacggttc cgtttacgac gagctcgggc agggcgtcga gcgcgaccgt ggcgcgatcg
     1501 ccgactgcga tccggacaac ctccagctcc gacaggtcgg ttgtctcgat gagccagccg
     1561 gactgatcac ccaccgtgat gacggcctgg cctgccagcg cctgttcgcc cagctcgatg
     1621 tcgacgctgg cgaccgtgcc ggcgaacggc gccctgatct ccgtctgggt gagcgcggtc
     1681 tgcgcctccc tgagccgaga ttcggccagg tcgaggtccg cttcggccag tcgcaggctg
     1741 gcctggacga actcgatctg acctgggctc gcgtcaagcg gcagctgttc gacctgcaaa
     1801 cgcgctagct ccacggccct tccggcctgg tcgacccgcg actgagcgac atcgattgcg
     1861 tcctggtaag tcttctgctc gagcttgagt agcagctgcc cgtcctcgac cgggctgccg
     1921 ttgtgcacgt agatagaggc gaccacaccg ctgatgcgcg tcgccagctc ggcgctgcgt
     1981 agcgggacaa cgacggccgc ggcgccgact gagttgtcga tgggcgtcgg ctcggcggtg
     2041 atcgacggcg agggcctgac cgtcggaccg
//
LOCUS       DATLCO010000108         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1622662, whole genome shotgun sequence.
ACCESSION   DATLCO010000108 DATLCO010000000
VERSION     DATLCO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622662"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VMZ33_02650"
     CDS             <1..720
                     /locus_tag="VMZ33_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HUR16163.1"
                     /translation="NDIGYTLNGRKEYSSNLRYARHVIVAARTSESAMGGLSWFFVPM
                     DAEGVEIGTRWETLGLHAIDVSPLELTNVLVPVDRRLGDEGRGLWMFNEHLAQSRTGI
                     AAMAVGIARRARDVVLEFGLHRRIYGGKLTKMQDYRFRIAEMEMEIAAARSLVALSAQ
                     KSDAGLDHAKEASLAKLFAGQAVMRITSDAVLMLGSIGYTGQTVVDKLMRDARHVSIV
                     EGPDPIHKEIVFANILRRGSY"
     gene            complement(725..937)
                     /locus_tag="VMZ33_02655"
     CDS             complement(725..937)
                     /locus_tag="VMZ33_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16164.1"
                     /translation="MNEDPRNEAPGEAPYIGDEFADSTSGATIDETSMTEGVGGLPEV
                     EDKTRATVSDVDGDNVEPDPERKPQV"
     gene            complement(937..1332)
                     /locus_tag="VMZ33_02660"
     CDS             complement(937..1332)
                     /locus_tag="VMZ33_02660"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUR16165.1"
                     /translation="MKQARNPESVHAPTGRYVHQIEVSAEHRMLFISGQVGMEPGGDV
                     PPETTAQFEVALANVMANVEAAGFAPTDVVKLVTYVVDSTDMNPADRRAVLDQTFGDH
                     VTASTLVYVSRLAAPQYLVEIDAWAVKAD"
     gene            complement(1329..>2622)
                     /locus_tag="VMZ33_02665"
     CDS             complement(1329..>2622)
                     /locus_tag="VMZ33_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUR16166.1"
                     /translation="PMILEGRDVLAAAQTGTGKTAAFVLPILQRLQTLAYTQTSGRRP
                     IRVLVVTPTRELCLQVEEAVRVYSQTRPIRSTAIYGGVPFDPQLRKLLQAPEIVVATP
                     GRLLDHVEQKTIDLSRVEVLVLDEGDRMLDMGFIPDVRRIIALLPRQRQTLMFSATFS
                     AEVRKLASDFQVNPHFIQIEPKQTSAESVRQVVIPVDQHRKRDLLAHMIKSGRIEQAL
                     VFTRMKHAAGRLADQLERDGIRATAIHGDKSQIQRNRALEDFKTGKVDILVATDVAAR
                     GLDIDALPHVINYELPTVAQDYVHRIGRTGRAGMDGDAISFVAPGEEDYLFDIQRLLK
                     HDIDIEVIREFLPEDPDSQPRSRREAPEPRMRGFRESSPRGGSRSARGYSGAVGGSMP
                     VSRPQSSGTSGPRPQSSGNQSGRPLTAMPGERLSRSSS"
BASE COUNT          422 a          807 c          922 g          471 t
ORIGIN      
        1 aacgacatcg ggtacacgct caacggccgc aaggagtaca gctccaacct gcggtacgcg
       61 cggcacgtga tcgtggcggc gcgaacttca gagagcgcga tgggcggcct gagctggttt
      121 ttcgttccga tggacgccga gggcgtggaa atcggcaccc gatgggagac ccttggcctg
      181 cacgcgatcg acgtttcgcc gctggagctg acgaacgtgc tcgtacccgt ggatcgccgc
      241 ctcggcgacg agggccgcgg cttatggatg ttcaacgagc acctcgctca gtcgcgcacc
      301 ggaattgccg cgatggccgt ggggattgca cggcgggcac gcgacgtcgt gttggagttc
      361 ggactccatc ggcgcatcta cggcggcaag ctgacgaaga tgcaggacta ccggttccgc
      421 atcgcggaga tggagatgga gatcgccgcg gcgcggtcgc tggttgcgct ctctgcccag
      481 aagtcagacg ctggcctgga tcacgccaag gaggccagtt tggccaagct gttcgccggt
      541 caggccgtga tgcgcatcac gtctgacgcc gtcctgatgc tggggagcat cggctacacg
      601 ggtcagacgg tggtcgacaa gctcatgcgg gacgctcgcc acgtgtccat cgttgaggga
      661 cccgacccga tccacaagga gatcgtgttc gccaacatcc ttcgccgcgg ctcgtactag
      721 ggaactaaac ctggggcttg cgctccgggt ccggctcgac gttatcgccg tcgacgtcgc
      781 tgacggtggc gcgggtcttg tcctcgacct ccggcaggcc gccgacgcct tcggtcatcg
      841 acgtctcgtc gatcgtcgcg ccagaggtgc tgtccgcgaa ctcgtcaccg atgtagggcg
      901 cttcgccggg cgcttcgttg cgcgggtcct cgttcatcag tcggccttga cggcccaggc
      961 gtcgatctcg accaggtatt ggggagcggc taaccggctg acatagacca gtgtcgaggc
     1021 agtgacgtgg tcgccgaacg tctggtccag caccgcacgg cgatcggccg ggttcatgtc
     1081 cgtgctgtcg acgacgtagg tcaccagctt gacgacgtcc gtcggcgcga aacctgccgc
     1141 ctcgacgttc gccatcacgt tcgccagggc gacctcgaac tgggcggtcg tctcaggcgg
     1201 cacatcgccg ccgggctcca tccccacctg gcccgagatg aacagcatcc ggtgctcggc
     1261 ggagacttcg atctggtgaa cgtagcgtcc agtgggcgcg tgaaccgact cggggttgcg
     1321 tgcctgtttc acgaactgga ccgggagagg cgttcacccg gcatggcagt gagcgggcga
     1381 ccggactggt tgccggacga ttgcggccgc ggacccgagg tcccggacga ctgcggccga
     1441 ctgaccggca tgctgccgcc aacagcgccg ctgtagcctc gcgcacttcg actcccgccg
     1501 cgcggcgagg actctcggaa gccacgcata cggggttccg gcgcctctcg acgtgatcga
     1561 ggctggctgt cggggtcctc tggcaggaac tcccggatga cctcgatgtc gatgtcgtgc
     1621 ttgagcaggc gttgaatgtc gaagaggtaa tcctcctcgc ccggtgcaac gaagctgatc
     1681 gcgtcgccgt ccatcccggc gcggcccgtt cggccgatgc ggtgaacgta gtcctgcgcg
     1741 acagtcggca gctcgtagtt gatgacgtgg gggagggcgt cgatatcgag cccgcgtgcc
     1801 gccacgtcgg tcgccacgag gatgtcgacc ttgcccgtct tgaagtcctc aagtgcgcgg
     1861 ttgcgctgga tctggctctt gtcgccgtga atggcagtgg cgcggatgcc atcgcgctcg
     1921 agctgatcgg caagtcggcc agcggcgtgc ttcatccgcg tgaagacgag cgcctgctcg
     1981 atccgacctg acttgatcat gtgcgcaagc agatcgcgct tccgatgctg gtcgaccggg
     2041 atgaccacct ggcgaacgct ctcagccgat gtctgcttgg gctcgatctg gatgaagtgc
     2101 gggttgacct ggaagtcgga cgcgagcttg cgcacctcgg ccgaaaatgt ggctgagaac
     2161 atgagcgtct gccgctggcg cgggaggagg gcgatgatcc ggcggacatc ggggatgaaa
     2221 cccatgtcca gcatgcggtc gccctcgtca aggacgagca cctcaaccct ggagagatcg
     2281 atggtcttct gctcaacatg gtcgagtagc cggccgggcg tggcgacgac gatctccggt
     2341 gcctgaagga gcttgcgcag ctgcgggtcg aacgggacgc cgccgtagat cgcggtcgat
     2401 cggatggggc gggtctggct gtaaacgcgg accgcttcct cgacctggag gcagagttcg
     2461 cgggtcgggg tgacgacgag gacacggatg ggtcgtcggc cgctcgtctg ggtgtaggca
     2521 agggtctgga ggcgctgcag gatgggcagc acgaacgccg ccgtcttgcc cgtgccggtc
     2581 tgggcggcgg cgagaacgtc gcggccttcc aggatcatgg gg
//
LOCUS       DATLCO010000109         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_132421, whole genome shotgun sequence.
ACCESSION   DATLCO010000109 DATLCO010000000
VERSION     DATLCO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132421"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /gene="atpB"
                     /locus_tag="VMZ33_02670"
     CDS             <1..823
                     /gene="atpB"
                     /locus_tag="VMZ33_02670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HUR16167.1"
                     /translation="ELPAPHVIYDFTPNTPPSTDLATFDLTISSSLFTGWIVMLIIIV
                     LAFLMSRGMSVSPKGRQNALEYVYEGLANFATSLGGPKARRYVPAFVAFFLFILLSNW
                     SGLFPGVGRTEQLRAPTSDVNTTIGLALVAFFMFHIEGVRALGVRGYLGKFFSLKAFK
                     DGIGAGVIALFVGVIEFFLEFIKPVTLAMRLFGNIYGGEVALGVITGLTIALIPVAMV
                     GLEFLLNFMQALIFAVLTLMFTIIATESHDDHEEHEVPEGNIAPPIAHGQPAAAH"
     gene            903..1127
                     /locus_tag="VMZ33_02675"
     CDS             903..1127
                     /locus_tag="VMZ33_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HUR16168.1"
                     /translation="MEGNIGLLGAGLAALGVIGPGIGIGILTGLSTAAIGRNPDAAAQ
                     IRATFIIGAAFAEGLGVLAVVVGLLAIFIG"
     gene            1137..1721
                     /gene="atpF"
                     /locus_tag="VMZ33_02680"
     CDS             1137..1721
                     /gene="atpF"
                     /locus_tag="VMZ33_02680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HUR16169.1"
                     /translation="MDIFAVAGGAAEVISRILAQEEPHEVGFQINLFWIIAQAASFLI
                     FLVILYFVAFKRIGGVLEERRSRIEQGLRDADQARVDREQAAAERLRVLADARTEAED
                     ILVRAQRVAEESRERDMAETRAQLEQMRERAAADIVSEKERALADVRSQVADLALAAA
                     ARVVGETMNTDRERRLVEEFLNEVGTATLPGRQN"
     gene            1738..>2106
                     /gene="atpH"
                     /locus_tag="VMZ33_02685"
     CDS             1738..>2106
                     /gene="atpH"
                     /locus_tag="VMZ33_02685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HUR16170.1"
                     /translation="MAGRRASPARRYAEAAFQIAERDGNVGQWLAQLGDLAQAVAGPS
                     VVRRLEDPNIPLDDRLAALRSTLGDDAVPQLVNMARLLLRRRRADEIRGVYREFKRLY
                     NRQAGIVEASATSAVELDDQE"
BASE COUNT          352 a          707 c          679 g          368 t
ORIGIN      
        1 cgagctaccc gcgccgcacg tgatctacga cttcacccca aacacgccgc cgtcgaccga
       61 tctggccacg ttcgacctga cgatctccag ctcgctcttc acgggctgga tcgtgatgct
      121 gatcatcatc gtgctggcct tcctgatgtc gcgcggcatg agcgtctcgc ccaagggtcg
      181 ccagaacgcg ctcgagtacg tctatgaagg cctcgccaac ttcgccacct cgctcggcgg
      241 acccaaggcg cggcgatacg tacccgcatt cgtggccttc ttcctgttca tcctgttgag
      301 caactggagc ggcctcttcc cgggcgtcgg acgaacggag cagctgcgcg cgcccacgag
      361 cgacgtgaac acgacgatcg gcctcgcgct ggtcgcgttc ttcatgttcc acatcgaagg
      421 cgtgcgagcg ctcggcgttc gtggctacct cggcaagttt ttcagcctca aggccttcaa
      481 ggacggcatc ggcgccggcg tcatcgccct gttcgtaggc gtgatcgagt tcttccttga
      541 gttcatcaag ccggtcacgc tggcgatgcg actcttcggc aacatctacg gcggcgaggt
      601 cgccctgggc gtgatcaccg gacttacgat tgcgctcatt ccggtcgcca tggttggcct
      661 ggaattcctg ctcaacttca tgcaggcgtt gatcttcgcg gtgctgacgc tgatgttcac
      721 tatcatcgcc acggagagcc acgacgatca cgaggagcac gaagtccccg aagggaacat
      781 cgcgccgccg atcgctcacg ggcagccagc ggcagcccac taggacaagc cctcacacca
      841 acccccatca actcagcgga caatgcccgg cgctggccgg gacgaaggag gaagagacca
      901 caatggaagg aaacattggc ctgctcggag ctggcctggc cgccctcggg gtcatcggcc
      961 caggcatcgg gatcggcatc ctgaccggcc tgtcgacggc cgcgatcggc cgcaaccctg
     1021 acgccgcggc ccagatccgc gccacattca tcatcggcgc agcgttcgct gagggactgg
     1081 gcgtgctcgc ggtcgtcgtc ggtcttctcg cgatcttcat cggttagccc gtcgcggtgg
     1141 acatctttgc ggtagccggt ggcgctgccg aggtcatctc gcggatcctc gctcaggagg
     1201 agccacacga ggtcgggttc cagatcaacc tgttctggat cattgcccag gccgcgtcgt
     1261 tcctgatctt cctggtgatc ctctacttcg tcgcattcaa gcgaatcggc ggagttcttg
     1321 aggagcgccg gtcgcggatc gagcaggggc tgcgcgacgc cgatcaggca cgcgtcgacc
     1381 gagagcaggc cgcggccgag aggctgcgcg tcctcgcgga tgcgcggact gaggccgagg
     1441 acatcctcgt tcgggcgcag cgtgtcgccg aggagtcgcg cgagcgcgac atggccgaga
     1501 cacgggccca gctcgagcag atgcgtgagc gcgcggcggc ggacatcgtg tccgagaagg
     1561 agcgcgccct tgccgatgtt cgttcacagg tcgccgacct ggcactcgcc gccgcggcgc
     1621 gtgtcgtggg cgagaccatg aacaccgatc gtgagcgacg tctcgtcgag gagttcctca
     1681 acgaggtcgg cacggccacg ctgccgggcc ggcagaacta ggcacgggtc ccaccagatg
     1741 gcaggcagac gggcgtcacc ggcacgccgg tacgcggagg ccgcgtttca aatcgccgag
     1801 cgcgacggca acgtcgggca gtggcttgcc cagctgggtg acctcgcgca ggccgttgcc
     1861 ggtccgtccg tggtgcgtcg tctcgaggat cccaatatcc ccctcgacga ccgcctcgcc
     1921 gctctgcgct cgacgctcgg agatgacgca gttccacaac tcgtgaacat ggcgcgcctg
     1981 cttctgcggc gtcgccgagc agacgagatc cgtggcgtct atcgcgagtt caagcgcctg
     2041 tacaaccgtc aggcgggcat cgtcgaggcg agcgccacca gcgcggtcga gctcgacgac
     2101 caggag
//
LOCUS       DATLCO010000110         3491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1624155, whole genome shotgun sequence.
ACCESSION   DATLCO010000110 DATLCO010000000
VERSION     DATLCO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624155"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VMZ33_02690"
     CDS             complement(<1..884)
                     /locus_tag="VMZ33_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HUR16171.1"
                     /translation="MADRTGALGTITHAEAALRAKSIPRVVLVWFLVVLLITGVGIAG
                     YVLLGGWSFADALYMTGITLTTVGFREVHPIEPASPVGIWTLLVAMAGVGLIFGTVGI
                     VAESFLAEAVSGRRAHRKMAHRIEQLTGHFILCGYGRVGSTVARELAHSNLDFVVVDI
                     NPQSLEQASDEKMLVVEGDATHDDVLLRAGVERAGGLITTTDSDANNVYVTLSARALN
                     KALFIVARANEEGADDKLRQAGADRVVSPYARAGRQIAELATRPRVADFIDFALSHGN
                     LSFSMEEIEVADGGLAGET"
     gene            complement(986..1061)
                     /locus_tag="VMZ33_02695"
     tRNA            complement(986..1061)
                     /locus_tag="VMZ33_02695"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Pro,seq:tgg)
     gene            1128..2018
                     /locus_tag="VMZ33_02700"
     CDS             1128..2018
                     /locus_tag="VMZ33_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR16172.1"
                     /translation="MKVGACLPIGERGAERQAFGYGSMRDMAIAAEHGGLDSIWVADH
                     LFFDSGDGNIRGVWESMAMLGALAEATTRVELGPLVLCTPFRNPGMVAWAANSLDEIS
                     GGRFVLGLGSGWHQPEFDAFGFEFTRRVSVFADSLEVIVPLLREGAADYEGDFAVGRA
                     ELRPPGPRRAGPPIMIAGTKPRMMGLIARFADRWNDVWFGLPTEEFRDNRARLVEACE
                     AIGRDSSTIEVSAGIQIKDPRTVDVNDGEQIVGRAEHIAEALAAWQAEGVDEVMCRLE
                     PPSVGMIEEIADATARFRAT"
     gene            2034..>3491
                     /locus_tag="VMZ33_02705"
     CDS             2034..>3491
                     /locus_tag="VMZ33_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HUR16173.1"
                     /translation="MRDARVRDFGRLLGVAVPTLALATAAIAVLQDRLGVPNPSAVYL
                     VAVVATAIISGTLGAIATSVAAFVLYTYLFTEPRNTFHIEDPGVLVSVLLLLFVGVVV
                     GQLTARQRSRADAARQREQEARALFSISRRLVTRDSVQAVLGEICAALQREARMSAVW
                     VTLGLPEGGDPIGVVNQLRRMPAETPPQWVRVHQPAGRARTHKDRDTFRVAIESGTDM
                     VGAIWASRPRDAGPPNAHETRLLAATADQLGQAIAHDRMAADSQSAEVARESDALKSA
                     LLQSVSHDLRTPLATIRAAAGTLRSGTVDFHADSDGHESLDAIDREVEYLNRLVTNLL
                     DLSRIEAGVLRPELDTFELDDLVRPTIERLDARLAGWTLQVQVGAYLVEADPVLFDAV
                     LTNVLENSVRHTMPGTTIRLSSADPGEMTRLIIEDSGTGVTDDVLDTIFEKFYRAPTR
                     NSSRSGTGIGLAVARGFVEAIGGRIEAHRSKLGGLA"
BASE COUNT          589 a         1150 c         1165 g          587 t
ORIGIN      
        1 gtttcaccgg ccaagccgcc atcggcgacc tcgatctcct ccatcgaaaa cgagaggttt
       61 ccatgcgaca gcgcgaagtc gatgaagtca gccactcgcg ggcgcgtcgc cagctccgcg
      121 atctgccgcc cggcccgcgc atagggcgac acgacacggt cggcccccgc ttggcgcagc
      181 ttgtcgtcgg cgccctcttc gttcgcgcgc gcaacgatga atagggcctt gttcagagcg
      241 cgcgccgaaa gcgtcacgta gacgttgttg gcgtccgagt cggtggtcgt gatcagaccg
      301 ccggcgcgct ccactccggc gcgcagcaat acgtcatcgt gcgtggcgtc gccttcgacg
      361 accagcatct tctcgtcgct cgcctgctcg aggctctgcg gattgatgtc gacgaccacg
      421 aagtcgaggt ttgaatgggc cagctcgcgc gcgacggtcg aacccacgcg gccatacccg
      481 cacaggatga aatgaccggt tagctgctcg atgcggtgag ccatcttgcg gtgtgcgcgc
      541 ctcccactca cggcctcagc caagaacgac tcggccacga taccgactgt gccgaagatt
      601 aggccgacgc cggccatggc cacgagcaac gtccagatcc cgacgggcga ggccggctct
      661 atcggatgga cctcgcggaa gccgaccgtg gtcagcgtga tcccggtcat gtagagcgcg
      721 tcggcgaacg accaaccgcc gagcaagacg tagccggcaa tgccgacgcc tgttatgagc
      781 aggacgacga gaaaccagac gaggaccact cggggaatcg atttcgctcg gagggcagcc
      841 tcagcgtgcg tgatcgttcc gagagctccg gttcggtcgg ccattgggct ggatggtagt
      901 cgggctcagc gcgagttcga gagccgtaca agcgacctgg cctcaatacc cgagcgcggc
      961 gaatttcccg ccgggacaga tggagtggtc ggggtggtgg gactcgaacc cacgacctca
     1021 tgctcccaaa gcatgtgcgc taccaactgc gctacacccc ggcgaggtca tgatagggcg
     1081 cgttacccgg cgcgccgcta ggctgcaaac cccatgaagg tcgactgttg aaagttggtg
     1141 catgcctgcc catcggcgag cgtggcgccg agcggcaagc gttcggctat ggctcgatgc
     1201 gcgacatggc gatcgctgcc gagcacggcg gactggactc catctgggtc gccgaccacc
     1261 tgttctttga ctccggcgac gggaacatcc gtggggtatg ggagtcgatg gccatgcttg
     1321 gcgcgctcgc cgaggcaacg acccgcgttg agctcggacc gctcgtcctg tgcacgccgt
     1381 tccgcaaccc gggaatggtc gcctgggcgg caaacagcct cgacgagatc agcggcggcc
     1441 gcttcgtact tggccttggg agtggctggc atcagcccga attcgacgcg tttggcttcg
     1501 aattcacacg acgcgtcagc gtattcgccg actcgttgga ggtgatcgtt ccgctgctgc
     1561 gcgagggcgc ggccgattat gaaggcgatt ttgcggtcgg aagggcagag ctgcgaccgc
     1621 cgggaccgcg ccgcgccggg ccaccgatca tgatcgccgg cacgaagccg cggatgatgg
     1681 gcctcatcgc gcgctttgcc gatcgctgga acgacgtgtg gttcggcctt cccaccgagg
     1741 agttccgcga caaccgggcg cggctcgtcg aggcgtgtga ggcgatcggg cgcgactcct
     1801 cgacgatcga ggtcagcgcg ggcatccaga tcaaggaccc gcggacggtc gatgtgaacg
     1861 acggcgagca gatcgtcggc cgggccgagc acatcgccga ggcgctggcg gcgtggcagg
     1921 cggagggcgt tgacgaggtc atgtgccgcc tggagccgcc gtccgtcgga atgatcgagg
     1981 agatcgccga cgccaccgcg aggttccgcg cgacctagct ctacgatcgc gacatgaggg
     2041 acgctcgcgt gcgcgatttt gggcggcttc tcggcgttgc cgtgccgacg ctggccctcg
     2101 cgacggcggc gatcgcagtg ctacaagaca ggctcggcgt ccccaaccca tccgctgtct
     2161 atctcgtcgc ggtcgttgcc acggccatca tttccggcac gcttggcgca atcgccacgt
     2221 ccgtggccgc gttcgtgctc tacacctacc tgttcaccga gccgcggaac acattccaca
     2281 tcgaggaccc cggtgtcctc gtaagtgtcc tgctgctctt gttcgtcggc gtggtggtcg
     2341 ggcagctcac cgcacggcag cggtcccgtg ccgacgccgc acgccagcgc gaacaggagg
     2401 cacgcgccct gttctctatc agccgccggc tggtaacgcg tgattccgtc caggccgtgc
     2461 tcggcgagat ttgcgccgcc ctacagcggg aggcgcggat gagtgccgtc tgggtcactc
     2521 tcggcctgcc cgaaggtggc gatcccattg gggtggtcaa ccagctacgg cgcatgccgg
     2581 cggagacgcc gccccagtgg gtgcgcgtcc accagcctgc cggacgggca cgcacgcaca
     2641 aggatcgcga cacgttccgg gtagccatag agtcgggcac cgacatggtc ggcgccatct
     2701 gggcgtcgcg gccgcgcgac gctggcccgc ccaacgcaca cgagacgcgg ctgctagcgg
     2761 cgaccgccga tcagctggga caagcgatcg cccatgaccg gatggcggct gactcgcagt
     2821 cggcagaggt cgctcgggaa agcgatgcgc tcaaatccgc cttgctccag tctgtttcgc
     2881 acgatttgcg cacgcctctg gcaacgattc gggcagccgc cggcacctta cgctcgggca
     2941 cggtcgactt ccacgctgat agtgacggtc atgagagcct ggacgcaatc gatcgcgagg
     3001 tggagtacct caaccggctg gtgacgaacc tgctcgacct gagccgcatc gaggccggcg
     3061 tcctgcgccc ggagctggac acgttcgaac tggacgacct ggttcggccg accatcgaac
     3121 gcctggatgc acggctcgcg ggctggacac tccaagtgca ggtcggggcg taccttgttg
     3181 aggccgatcc cgtcctcttc gatgccgtgc ttaccaacgt tcttgaaaat tctgtccggc
     3241 acaccatgcc cggcacgacc atcaggctct cctcggccga tccaggcgaa atgactcgcc
     3301 tgatcatcga ggactcgggc accggagtga ccgacgacgt cctggacacc atcttcgaga
     3361 agttctaccg cgccccgacc cggaattcgt cgcggagcgg cacgggcatc gggcttgcgg
     3421 tcgcgcgcgg attcgtcgag gccatcggcg ggcggatcga agctcatcgg tcgaagctcg
     3481 gtggactggc g
//
LOCUS       DATLCO010000111         5731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_879861, whole genome shotgun sequence.
ACCESSION   DATLCO010000111 DATLCO010000000
VERSION     DATLCO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879861"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VMZ33_02710"
     CDS             <1..591
                     /locus_tag="VMZ33_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR16174.1"
                     /translation="DEERRNEVISDIRVEAERLARLVEDLLVMSRVERGNVDIGEEPI
                     LIQRLLPSVVQSFTGQWPDVHVNMNLGPRLSAVRGDTTYVEQVVRNLLTNAARYGKGA
                     TGGIELTAEEGEDDVIVRVLDRGDGFGDVNPERLFELFYRTDTARSVPGGAGIGLFVV
                     RNLIEAMGGRIWARSREGGGAEFGFTLPVLESDSSY"
     gene            complement(595..1065)
                     /locus_tag="VMZ33_02715"
     CDS             complement(595..1065)
                     /locus_tag="VMZ33_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUR16175.1"
                     /translation="MDVLTLLKEDHDRVKKLLEEGDSTTERGEKTRTEIFARLKTMLT
                     AHEAMEEEILYPALKAHPQAEELTLEAYEEHHVVDLILEELEMTPVTDEQWGAKFTVA
                     KENIEHHIEEEEGEMFKMIRQAFSADEREQMGAQMAQIQALAKQVVGEEMAQTL"
     gene            complement(1617..2873)
                     /locus_tag="VMZ33_02720"
     CDS             complement(1617..2873)
                     /locus_tag="VMZ33_02720"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16176.1"
                     /translation="MPLIPALLHRPAFRNLWLGQTISVFGDQITLLAIPIIAVLVLNA
                     EPEQMGLLTAAGLLPHLLFSLPAGVWLDRVHRRRRLMVWADIARAVMIATIAVAFMLN
                     ALSLAQMFIVTFIVGTLAVAFDISWSTVYVKVVDRPEYVPANSLLNGSRSLAYVAGPS
                     VGGVLIQFLTAPIAVLVDAASYVLSAFFVWRVDAPEPTVEPQTDSIRRQLSTGLSFLA
                     RDPIMRPTLASVATLNFFNWAFTALFVLYVTTRLGVSAGELGLALGAGAIGGVVGALL
                     AARIGRRIGIGRAFLIGMIVFPIALVAVPLTPPGTPVPLVIAMLVGMEFVAGFGVMVL
                     DINASAIIPARTPDGIRSRVSGAWRFVNMGIRPIGAIVGGLLGGLIGVQETLLATSLL
                     QLVGVLFLLGTPVLRLHDVPEAAEHP"
     gene            2904..2978
                     /locus_tag="VMZ33_02725"
     tRNA            2904..2978
                     /locus_tag="VMZ33_02725"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2936..2938,aa:Gln,seq:ctg)
     gene            complement(3255..3629)
                     /locus_tag="VMZ33_02730"
     CDS             complement(3255..3629)
                     /locus_tag="VMZ33_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16177.1"
                     /translation="MRISLRLILASAVVAAGMISTVGPALGADTITVSCTNGFQRTVA
                     AQAARGVAKSLTKFNEHRGSSVTCAAAPGAPRSRPASAWLTISCTNGFERTVNARAAG
                     GITRALNAFNTRSQTGVTCAAA"
     gene            complement(3971..4378)
                     /locus_tag="VMZ33_02735"
     CDS             complement(3971..4378)
                     /locus_tag="VMZ33_02735"
                     /inference="COORDINATES: protein motif:HMM:NF020839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUR16178.1"
                     /translation="MRIGELASKLGTTPHTIRFYEKNGLLPAASRAANSYREYDDSDL
                     SRLRLLVGLRSLDLPLGQAAELASLCADGRCEQVSTEARAAIAEKRVELARRMEELTY
                     LDRRLAHLCGELTAGKKLKNAITDGRRNVDDPL"
     gene            4432..4914
                     /locus_tag="VMZ33_02740"
     CDS             4432..4914
                     /locus_tag="VMZ33_02740"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HUR16179.1"
                     /translation="MLKTTEVVSMEKQRVLFVCTHNSARSQMAEGFLRHFAGDQFEVH
                     SAGTEASNVRPLAIRAMAEIGVDISGQESKLLDRYLDDRWDHVVTVCDDANESCPVFP
                     GATNRGHWSFTDPSKAEGTEQERLAVFCKVRDQIVQRVRLFALAAGPRKTPTALLSHE
                     "
     gene            complement(4979..5488)
                     /locus_tag="VMZ33_02745"
     CDS             complement(4979..5488)
                     /locus_tag="VMZ33_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HUR16180.1"
                     /translation="MLTVVVSLPAAIVAGSVALAGFGLDSGVEVGAGVVALAALRGNP
                     AANRARHVLLLRLAFVAIAIYLIAQAAYVFLSDDRPEPTAVGIGLLLLTVIVMTLLAI
                     AKRRVSVQLGNAAVAAEARITLIDSADSALVLAALVANAAFGWWWADALGGLALAAYS
                     LWEAFETRS"
     gene            complement(5548..>5731)
                     /locus_tag="VMZ33_02750"
     CDS             complement(5548..>5731)
                     /locus_tag="VMZ33_02750"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HUR16181.1"
                     /translation="ISPIDLVPEFIPVLGPLDDVVVAVVAMRFVRRRIGTEALMARWP
                     GSVDGFALLTRVIGSN"
BASE COUNT         1066 a         1855 c         1847 g          963 t
ORIGIN      
        1 gacgaggaac ggcgcaacga agtcataagc gacatccgcg tcgaggccga gcgactcgcc
       61 cgactcgtgg aagacctcct ggtcatgagc cgcgttgagc gcggcaacgt cgatatcggc
      121 gaggagccca tcctcatcca gcgcctgctc ccgtccgtcg tgcagagctt caccggacag
      181 tggccggacg tgcacgtgaa catgaacctt ggtccgcgtc tgtccgctgt ccgtggcgac
      241 acgacgtacg tcgaacaggt tgtgcgcaac ctgctcacga acgcagcgcg ttacggcaag
      301 ggcgctaccg gcggcattga gctcaccgcc gaagaaggcg aggacgatgt catcgtccgc
      361 gtgctcgacc gcggtgatgg cttcggggac gtcaaccccg agcgcctatt cgagctcttc
      421 taccgcaccg ataccgcacg gtctgtcccg ggcggggctg gaatcggcct tttcgtggtc
      481 cgcaacctca tcgaggccat gggcggacgc atctgggcgc gctcccggga gggtggcggt
      541 gccgagttcg gcttcaccct cccggtgctg gagagcgact cgagctactg acggctagag
      601 ggtctgggcc atctcctcgc cgacgacctg cttcgccagg gcctggatct gggccatctg
      661 ggcacccatc tgctcgcgct catcggctga gaaagcctgg cggatcatct tgaacatctc
      721 gccctcttcc tcttcgatgt ggtgctcgat gttctccttg gcaacggtga acttggcgcc
      781 ccactgctcg tcggtgacgg gcgtcatctc gagctcctcg aggatcaggt cgacgacgtg
      841 gtgctcctcg tatgcctcga gcgtgagctc ctcagcctga ggatgggcct tcagggccgg
      901 atagaggatc tcctcttcca tcgcctcatg ggcggtcagc atcgtcttga ggcgggcgaa
      961 gatctcggtt cgggtcttct cgccacgctc cgtcgtcgag tcgccctcct cgagcagctt
     1021 cttcactcga tcgtggtctt ccttgagcag cgtaagtacg tccatgtctc tcccgttcgc
     1081 gctaggcggt catgacctgc aggtctgttg cgatgaccgt aggcgggacg tgatgccggg
     1141 cgttcgatcc agtcccgctc cgacgtttcg cctcgattgc aacacggtgc gttcgtccga
     1201 ttagggcttg aggcggccgc cggcctaggt tccccgacat gcctgagaac gcatggggcc
     1261 cgaagcggga gcgccaatgc acgcacatgt tcctggcaag gacaaccgga cgaaattggg
     1321 gtcgcttgcg ctgccgtttg gccaattcga cagccgattt cgcttgcgga cgactcacat
     1381 ggccgggaaa tcacgcattt gggacgggat cggcctcatc gggagccggg ttttcgccga
     1441 gtggtatcca cgaggaacag tccggtcgcc gcagtggctg gcgaacccct cgacggaccg
     1501 tcgtcgtcag ggccgtgtcc gcgctcgatg ttcgcggcac gcacgttgcc agcgggaggg
     1561 aaccgatgtg cgtgaggtgg acgtacctcg gatcgccaac aaggcgagac ccacgcctag
     1621 gggtgttcgg cggcctccgg cacgtcgtgc aggcgcagca cgggcgtccc cagcaagaac
     1681 aggaccccca cgagctgtag cagcgacgtc gccagcagcg tctcctggac cccgatcaac
     1741 ccgccgagaa gcccgccgac gattgcgccg atagggcgga tacccatgtt gacgaagcgc
     1801 cacgcgccac tgacgcggct gcggatcccg tccggagttc gtgccgggat gatggcgctg
     1861 gcgttgatgt cgaggaccat gacgccgaat ccggcgacga actccatgcc gaccaacatc
     1921 gcgatgacga gcggcacggg cgtgccgggc ggcgtgagcg gaacggccac aagcgcgatc
     1981 gggaagacga tcatgccgat caggaacgcc cgaccgatcc cgattcggcg cccgatccgg
     2041 gccgccaaca gcgcgcccac gacgccgccg atcgcgccgg cgccaagagc caggccgagc
     2101 tcgccggcac tgactccgag gcgcgtagtc acgtacagga cgaacagcgc cgtgaaggcc
     2161 cagttgaaga agttcagcgt tgccacggag gcgagggtgg gccgcatgat cgggtcccgc
     2221 gccagaaacg acagccccgt cgacaactgt cgtcggatgg agtccgtctg gggctcaacg
     2281 gtcggctcgg gcgcgtcgac ccgccagaca aagaaggcgg acaggacgta cgaggcggcg
     2341 tcgaccagga ccgcgatggg cgcggtcaga aactggatga gcacgccgcc gaccgacggc
     2401 ccggccacgt aggcgagcga ccgactgcca ttcaggagcg agttcgccgg cacgtactcg
     2461 gggcggtcga cgaccttgac atagacggtg ctccacgaga tgtcgaaggc aacggccagc
     2521 gtgcccacga tgaacgtgac gatgaacatc tgcgccagtg acagggcatt gagcatgaat
     2581 gcgacagcga tcgtggcgat catcaccgcg cgcgcgatgt cagcccagac catcagccgg
     2641 cggcgccgat ggacgcggtc gagccaaacg ccagcaggaa gcgagaacaa gaggtgcggt
     2701 agcaggccgg cggctgtgag caggcccatc tgctcgggct cagcgttgag caccaggacg
     2761 gcgatgatgg gaatcgcgag gagcgtgatc tgatcgccga acacggagat cgtttggccc
     2821 agccacagat tccggaacgc agggcgatgc agaagcgcgg ggattaaagg caacgacacc
     2881 ccttggtaga ctggtcaggt cgctggcgag tggccaaacg gtaaggcacc tgattctgga
     2941 tcaggggatt gaaggttcga atccttcctc gccagccaag tccccgatac ctccgcctgc
     3001 gtgatcagca cgtccatgcc cgcttgccct atttttggct gggccgtaag ggcaggcttg
     3061 agccgcgggg cgtcgttaag gcgctgaagc gccgagccgc cgccgccgac atcacgaagt
     3121 tccacactcg ccccgccgtc atggcccaaa cagcgccatc tctcggtcca ctaaagacgg
     3181 cctgtctacc aaataaaaga gggccggagg tttccctccg gcccttcctg ttcggtgctg
     3241 gggcaccgag tttgtcaggc agcggcgcag gtgacgccgg tctggctcct agtgttgaac
     3301 gcgttgagtg cgcgggtaat gccgccggct gcgcgagcgt tgacagtccg ctcgaagccg
     3361 ttcgtacagc tgatcgtcaa ccacgccgag gccggtcgcg aacgcggtgc gccaggtgct
     3421 gcggcgcacg tgacgctgga cccccgatgc tcgttgaact tcgtgagtga cttcgccact
     3481 cctcgtgcgg cctgcgccgc gaccgtccgc tggaagccat tggtacagct gacggtgatg
     3541 gtgtccgcgc cgagcgctgg cccgacggtg ctgatcatgc ccgctgcgac gaccgcgctg
     3601 gcaaggatca atctcaggga aatcctcatc cctactcctt ttactgccgc tgggggcctg
     3661 cggcgtgaac gtcggctggc attctcctgt gaccgacccc cgcgatcaca gacacggcga
     3721 gcaatgcggc cctctccagc gacgatgctc ggcgatgcac ggagcggttg tcgatcccgc
     3781 ctagcgccag atggaccact tcggcgcctg acccttgccg atctccaagc aacaccgcgc
     3841 ccctactgga tcaggtaggt tgtcgcgtca acggtaggag taacaaccga cccggcctct
     3901 gcgcgaggcc gggtctcgat ccgcctactt agtgccgcaa ccgcagcagt gcttgtcgca
     3961 gcagcaatct tcacagtggg tcatcaacgt tcctccttcc gtcggtgatc gcattcttga
     4021 gcttctttcc ggccgtcagt tcgccgcaga ggtgcgcgag gcggcggtcg agataagtca
     4081 gctcctccat ccggcgcgcc aactccacgc gcttttcggc gatggccgct cgtgcctcgg
     4141 tggagacctg ttcgcagcga ccgtcagcgc acagcgaagc cagctcggcg gcctgcccca
     4201 gcgggaggtc gagagatcgc agacctacca gcagacgcag ccgtgagagg tcggaatcgt
     4261 cgtactcgcg gtatgagttc gcggcgcgac tcgcggcggg cagcaggccg tttttctcat
     4321 agaagcggat cgtgtgtggt gtcgtcccga gcttgctggc gagttctccg atgcgcatga
     4381 aaagagccta atccttagag ccgctctaat gtcaagcgcc gaatcgcgca tatgcttaag
     4441 accacggagg tggtgtcgat ggagaaacaa cgagtcctgt tcgtatgtac gcacaacagc
     4501 gcccgctcgc agatggccga aggcttcctc cggcacttcg cgggcgatca attcgaggtc
     4561 cacagcgcgg gcactgaggc aagcaacgtc cgtccgctcg ccatccgtgc catggccgag
     4621 atcggcgtcg acatcagcgg gcaggagagc aagctcctcg atcgctacct cgatgaccgc
     4681 tgggatcatg ttgtcaccgt ctgcgacgat gccaacgagt cctgccccgt cttccccggt
     4741 gcgacgaacc ggggccattg gagcttcacg gacccgtcga aggcggaggg taccgaacag
     4801 gagcgtctcg ccgtcttctg caaggtccgc gaccagatcg ttcagcgggt gcggttgttc
     4861 gcgctagcgg cgggtccgcg aaagacgccg acggcccttc tctcccacga gtgacagctc
     4921 taactaggcg cggcaccaga cccgcgctga tcgccaccgg caggcggcac agttccttct
     4981 agctccgcgt ctcgaacgct tcccacagcg agtacgccgc cagtgccagc ccgccgagtg
     5041 cgtcggccca ccaccagccg aacgcggcgt tggctactag tgccgccagt acgagcgccg
     5101 aatcggccga gtcgatcagc gtaatccgag cctcggcggc gacggcagcg ttgccgagct
     5161 ggacgctcac tcgacgcttc gcaatggcaa gcaacgtcat gacgatgacg gtaagaagga
     5221 ggagcccaat gccgactgct gtcggctcgg gcctatcgtc cgacaggaac acgtatgcag
     5281 cctgggcgat taggtaaatc gctatagcca cgaaggcgag gcgcaagagc agcacatggc
     5341 gcgcccggtt cgcggcggga ttgccccgca aggcggctag agccacaaca ccagcgccaa
     5401 cctcaacgcc agagtcgagt ccaaagcccg ccaacgcaac cgacccagcg actatcgcgg
     5461 ctgggaggct gaccacaacg gtcagcacat tccaagccat ggtcgcgtag accaaccttt
     5521 tggctttggg ggcgggattc acgatcttca gttcgagccg atgactcggg taagcagcgc
     5581 gaagccatct accgacccag gccagcgggc catcaaagct tcagtgccta tccgccgccg
     5641 aacgaagcgc atggcgacaa cagcgacgac cacgtcgtcc aacggtccga gaacgggaat
     5701 gaactcgggg acaaggtcga tcgggctgat g
//
LOCUS       DATLCO010000112         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1627894, whole genome shotgun sequence.
ACCESSION   DATLCO010000112 DATLCO010000000
VERSION     DATLCO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627894"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..240)
                     /locus_tag="VMZ33_02755"
     CDS             complement(4..240)
                     /locus_tag="VMZ33_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16182.1"
                     /translation="MQPSRPTGATTALAIALLVVGLALVFFQGQAVELVRGIGLPNDV
                     QRQIVQLMQERLAAFAALAASPILLIVGSLVRGI"
     gene            complement(301..636)
                     /locus_tag="VMZ33_02760"
     CDS             complement(301..636)
                     /locus_tag="VMZ33_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16183.1"
                     /translation="MHRGWINGGWLQPEDLGGELAAPPAVTTWAENEMEVFAIFPDGQ
                     LYDRYWDGKAWHDWESLGGELVGQPAASSWGKERLDVFATGHDGMLWHRWWNGTEWVP
                     WRQKLPPPA"
     gene            753..>2107
                     /locus_tag="VMZ33_02765"
     CDS             753..>2107
                     /locus_tag="VMZ33_02765"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16184.1"
                     /translation="MSARVRYAFDNYMSRGTIALILGLFLVSLLIIILVSFVVAVSGT
                     LLEADETRAIDLPRLMWMSLLRTLDPGTMGGDVGTGPFVFSMLAVTMGGIFIVATLIG
                     VISTGIQSKINELRQGRSIVLEDNHTVVLGWSTQIFDVIGEIVLANANKRNQRIVVLA
                     DRDKVDMETEIAQRIPNRKTTRIVCRRGSPMDLDDLALTSLQTARSIIVLSPEGDDPD
                     TGVIKALLAITNDPKRRQGPYQIVTEIRESRNAEVARLASRGEAQIVLGGELIGRIAA
                     QTCRQPGLSIVYMELLDFGGDEIYFHSDATLTGRQFGDALAAFRTSALIGLAPAQGAP
                     HLNPDTDTIIGAGDRLIFIAEDDDTIKREDAPGGMPHDAVIVVGQHARPAMERTLVLG
                     WNSRTPGLITELDRYVPPGSALLVIADGAGVADQIEAVGRRLSRLKLTFRPADTTDRD
                     VL"
BASE COUNT          376 a          702 c          660 g          369 t
ORIGIN      
        1 cgcctagatc cccctcacga gtgagccgac gatcagcagg atcggcgacg ccgccaaagc
       61 cgcaaatgcc gcgagtcgct cttgcatcag ctgcacgatc tggcgctgga cgtcgttcgg
      121 taatccgatt ccgcgtacaa gttcgaccgc ctgaccctgg aagaacacca gcgcgaggcc
      181 gactacgagg agcgcgatcg cgagcgcggt ggtggccccc gtcgggcggc ttggctgcat
      241 gtagttccct cttgagtgga cgcccagtct atcggcacgt cgagtggaaa tttcgcgcgg
      301 ctaggcgggc ggcggcaact tctggcgcca gggcacccac tccgttccgt tccaccaccg
      361 atgccagagc atgccgtcat gtccggttgc gaacacgtcg agccgctcct tgccccacga
      421 agacgccgcg ggctggccga cgagctcccc gccgagagac tcccagtcat gccaggcctt
      481 gccgtcccag tagcggtcgt acagctggcc gtcggggaat atggcgaaga cctccatctc
      541 gttctcggcc caggtcgtga cggcgggcgg cgcggccagc tcgccgccga gatcctcagg
      601 ctgcagccag ccgccgttga tccagccgcg gtgcatgagc tggcgctcag ggccgatcca
      661 gaagagatcg atgcgctcag cgccccaaga gacggaaatc agaccttcca ggcgctgtat
      721 tgtcggggcg cgtgaacacg aaccgtcatc cgatgtccgc gcgggttcgt tacgcgttcg
      781 acaactacat gtcacgcggc acgatcgcgc tgattctcgg ccttttcctt gtctcacttc
      841 tgatcatcat tttggtctca ttcgtggtcg cggtgtcggg gacgctgctt gaagccgacg
      901 aaacgcgggc tatcgacctg ccgcgcttga tgtggatgag ccttctgcgg acgctcgacc
      961 cggggacaat gggcggcgac gtcggaactg ggccgttcgt cttctcgatg ctcgccgtca
     1021 cgatgggcgg catcttcatt gtcgccacgc ttatcggcgt gatcagcacc ggcatccaga
     1081 gcaagatcaa cgagctgcga cagggccgtt cgatcgtcct cgaggacaac cacaccgtcg
     1141 tcctcgggtg gtcgacgcag atcttcgacg ttatcggcga gatagttctg gccaacgcca
     1201 acaagcgaaa tcagcgcatc gtcgtgctcg ccgataggga caaggtcgac atggagacgg
     1261 agatcgcgca gcgcatccca aatcggaaaa ctacgcggat cgtctgccgc cgcggatcac
     1321 cgatggacct tgacgacctg gcactcacca gcctccagac ggcgcgctcg atcatcgtcc
     1381 tgtcgccgga gggcgatgac cccgacacgg gcgtcatcaa ggcgctgctt gcaattacca
     1441 acgatccaaa gcgacgccag gggccgtacc agatcgtcac cgaaattcgg gagtcgcgga
     1501 acgctgaggt ggcccgcttg gccagccggg gagaggctca gatcgtgctc ggcggagagt
     1561 tgattggccg catcgccgcg cagacctgtc ggcagccggg cctgtcgatc gtgtacatgg
     1621 agctactcga cttcggcggt gacgagatct acttccactc cgacgccacg ctgaccgggc
     1681 gtcagttcgg cgacgcgctg gcggcgtttc gcacatcggc gctgatcggc ctcgcccccg
     1741 cacagggcgc gccacacctg aaccccgata cggacaccat catcggcgcc ggcgatcggc
     1801 tcatcttcat tgccgaagac gacgacacaa tcaagcgcga ggatgctcct ggtggaatgc
     1861 cgcacgacgc ggtgatcgtc gtgggccagc atgcgcggcc ggccatggaa cgaacgctgg
     1921 tcctcggctg gaactcgcgc acgcccggcc ttatcacgga gctcgaccgc tacgtgcccc
     1981 cgggctcagc acttctggta atcgccgacg gtgcgggcgt tgccgatcag atcgaggccg
     2041 tcggccggcg cctttcccgc ctgaagctga cctttcgtcc tgccgacacg accgatcgcg
     2101 acgtgct
//
LOCUS       DATLCO010000113         3109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5353561, whole genome shotgun sequence.
ACCESSION   DATLCO010000113 DATLCO010000000
VERSION     DATLCO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5353561"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VMZ33_02770"
     CDS             complement(<1..319)
                     /locus_tag="VMZ33_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HUR16185.1"
                     /translation="MLTSPDLDTLIADTESEILRVIEQAEDERTTELYDMVRYHLGLD
                     ADGARGKRLRPLLGLLAYDSIAGDHRQALPGAAAVELGHNFSLVHDDIEDNDVERHHR
                     ATLW"
     gene            420..1211
                     /gene="recO"
                     /locus_tag="VMZ33_02775"
     CDS             420..1211
                     /gene="recO"
                     /locus_tag="VMZ33_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HUR16186.1"
                     /translation="MPPPRVYYTEAIVLSRFELGEADRVLTLLTPHDGKLKAIAKGVR
                     RPKSRLGGAVEPFAELHLVMARARTFDVVTSASVGHAWLSLRERLESTATAWYLAELA
                     EKAVEERAFAYPVYALLRRAYQLLDDGMAPNRVARWFEFGLADALGVRPEVERCVECD
                     RVLESDDEFRWVPILGGTLCSRHLPPPSEQFPLSLAALKLLRAYRRLDIEAIAGLRQP
                     AAVEAEVEAALRSFIRTVLEREPRSLAFLDEVRARRTARAIATAV"
     gene            1391..1954
                     /locus_tag="VMZ33_02780"
     CDS             1391..1954
                     /locus_tag="VMZ33_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16187.1"
                     /translation="MRVSRARMLLALCFALTSIGVVAAPASAESVMVACNGRCGYYEV
                     RDSGPGNAGAQCNYGTSYPYKLTSIKVRPPLMHGDYPTKTKVGWSYRIQRRPVGGGNW
                     APISISGYQTALANDAIPAYAGSGFTFRRWFAPSNPAGYQYRVVIELQWWKNGSVEGY
                     ARVRYTWYHRVSGTNDDTQPNYCIQSF"
     gene            2067..>3109
                     /locus_tag="VMZ33_02785"
     CDS             2067..>3109
                     /locus_tag="VMZ33_02785"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HUR16188.1"
                     /translation="MTDDPKPSVGSLDTIVSLAKRRGFVFPSSEIYGGINAVWDYGPL
                     GVELKNNVKRAWWRAMVQERDDIVGVDAGILMHPQVWVTSGHVAEFSDPLVECQKNSH
                     RFRLDELPGTDSLTETQKRDPGIVEILGLKCPEDGSALGPPRRFNLMFKTFMGPVEEE
                     ANVVYLRPETAQGSYVNFKNVQQSTRKKLPFGIAQVGKSFRNEISPGNFVFRMREFEQ
                     MEMQYFVRPGDPAAAAFEEWLPKRRAWYERYGVTPSRLRFREHAADELAHYAKKAIDV
                     EYRFPFGWKELEGVHNRGDFDLGSHATASGENLEYFDQATEEHFIPWVVETAAGADRA
                     AFTFLIDAYREEEV"
BASE COUNT          548 a          973 c         1043 g          545 t
ORIGIN      
        1 tccagagggt ggcgcggtgg tggcgttcga cgtcgttgtc ctcgatgtcg tcgtggacca
       61 ggctgaagtt gtggccgagc tccaccgcag ccgccccggg cagtgcctgt cggtggtcgc
      121 cggcgatgga gtcgtacgcc aacaagccca gcagcggccg cagccgcttg ccgcgggctc
      181 catccgcatc gagccccagg tggtaccgca ccatgtcgta cagctcggta gtgcgctcgt
      241 cctctgcctg ctcgatgacg cgcagaatct ccgactcggt gtcggcgata agcgtgtcga
      301 gatcgggcga agtcagcact gtcggagcat atcgggagcg attggggcgt ccatcggtca
      361 atacatggta gcgtgctgta actagtaacg cccgagccgg atgcgggtac gctagggccg
      421 tgccgccacc gcgcgtctat tacaccgaag cgatcgtcct cagccggttt gagctgggcg
      481 aagctgaccg tgtcctgacg ttgctgactc cccatgacgg caagctcaag gcgatagcca
      541 agggcgtgcg acggcccaag agccggctcg gtggcgccgt tgagccattt gccgagctgc
      601 atctggtgat ggcccgggca cggacgttcg acgtggtcac atcggccagc gtcggccatg
      661 cctggctctc actccgggag cgactcgagt cgacggcaac agcctggtac ctggctgagc
      721 ttgccgagaa ggccgtcgag gagcgcgcat tcgcctaccc ggtctatgcc ctcctgcgcc
      781 gcgcctacca actgctcgac gatggaatgg cgcccaatcg agtggcgcgt tggttcgagt
      841 tcggcctggc ggacgcgctc ggcgtgcggc ccgaggtaga gcgctgtgtc gaatgcgacc
      901 gggtgctgga gtccgacgac gagttccgct gggttcccat tctgggcggg acgctgtgct
      961 cgcggcacct gcctccgcca tcggagcagt tcccactctc gctggctgcg ctgaagctgt
     1021 tgcgagcgta ccggcggctg gatatcgagg ccatcgcggg gctgcgccag cccgccgctg
     1081 tcgaggcgga ggtcgaggcg gcgttgcgca gcttcattcg gacagtgctg gagcgtgagc
     1141 cgcggtcgct ggcattcctc gatgaggtcc gcgctcgacg aacggcgcgc gcaattgcga
     1201 ccgccgtttg acgcaggacg aggcggcgat cctgaagcga aaaccgcgac ggcggctggc
     1261 tgctgacagc catctcgtcg tcggtggatg atgagccctg acgacctgct tgccctgcaa
     1321 ctggattgca accagactcg tcgaggatga cccggtcggg tcgtcaacca gccgtgaagg
     1381 gagcaacagg atgagggtta gtcgagcacg catgctgctg gcgctttgtt ttgcgctgac
     1441 gtccattgga gttgtggccg ctccggcgag tgccgaatcg gtcatggtcg cgtgcaatgg
     1501 caggtgcggt tactacgagg taagggacag cggccccggg aatgccggag cccagtgcaa
     1561 ctacggcacg agctatccat acaagctcac atcgatcaaa gtccgcccgc cgttgatgca
     1621 tggtgactat ccgaccaaga cgaaggtcgg ctggagctac cggatccaac gccggccggt
     1681 aggcggcggc aactgggcgc ccatttccat aagtggatac cagaccgcgc tcgcgaatga
     1741 cgcgatcccg gcctacgccg gtagcgggtt cacgttccgg aggtggttcg ccccgagcaa
     1801 cccggccggc tatcagtacc gtgtcgtcat cgagctccag tggtggaaga acgggtccgt
     1861 cgagggctat gcccgcgtcc gctacacctg gtaccaccga gtcagtggca cgaacgacga
     1921 tacgcagccg aactactgca tccagtcgtt ctgacagcaa cacgctatta cggaggcccg
     1981 acctcttggt cgggcctctt ttcgtgtgag aatgctgctt cgcgaccggc catgccacgc
     2041 gctggcgcgc tgagggagtt tgatccttga ctgacgaccc caagccgagc gtcggcagcc
     2101 ttgacacgat cgtgtcgctc gcgaagcggc gtggcttcgt gttcccatcg agcgagatct
     2161 acggtggcat caacgccgtc tgggattacg ggccactcgg cgtcgagctc aagaacaacg
     2221 tcaagcgggc gtggtggcgg gcaatggtgc aggagcgcga cgacatcgtc ggcgtcgacg
     2281 caggcatcct catgcacccg caggtgtggg ttacctccgg gcacgtggcc gagttcagtg
     2341 atccgctggt tgagtgccag aagaacagcc accgatttcg gctcgatgag ctgccaggca
     2401 cggactccct cacggaaacg cagaagcgcg acccagggat cgttgagatc ctggggctca
     2461 aatgccccga ggacggctca gccctcggac cgccgcggcg cttcaacctc atgttcaaga
     2521 ccttcatggg acccgtcgag gaagaggcga acgtcgtcta cctgcgtccc gagacggctc
     2581 agggctcata cgtcaacttc aagaacgtcc agcaatccac gcggaagaag cttccattcg
     2641 ggatcgcgca ggtcggcaaa agcttccgca acgagatctc tcccggaaac ttcgtctttc
     2701 gaatgcgcga gttcgagcag atggagatgc agtacttcgt caggccgggc gacccggccg
     2761 cggctgcgtt cgaggagtgg ctcccgaagc gcagggcctg gtacgagcgc tacggcgtga
     2821 cgccgtcgcg gctccgcttc cgggagcacg ccgccgacga gctcgcccac tacgcgaaga
     2881 aggcgatcga cgtcgagtac cggttcccgt tcggctggaa agagctcgag ggggtccaca
     2941 accggggcga tttcgacctc gggtcgcacg ccacggcctc gggcgagaac ctcgagtact
     3001 tcgaccaggc caccgaggag cacttcatcc cgtgggtcgt ggagaccgcg gcgggcgcgg
     3061 atcgcgcggc cttcaccttc ctgatcgacg cctaccgcga ggaggaggt
//
LOCUS       DATLCO010000114         9330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9757192, whole genome shotgun sequence.
ACCESSION   DATLCO010000114 DATLCO010000000
VERSION     DATLCO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9757192"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1641
                     /locus_tag="VMZ33_02790"
     CDS             295..1641
                     /locus_tag="VMZ33_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HUR16189.1"
                     /translation="MKLRYLGTFTATMVLVAACGGGGTATTRPSTAASTAASAAPSAA
                     QSAAPTQGGGGTLDTVKIGVDLPLSGASVANGQPTLQGVELAVKEWNEADHGYTVELN
                     VQDDAVNGVYNPEQGASNANTLVADEAVVGMVGPFNSSVARAIIPVTNEFGLAQCSPA
                     NTAIDLTKEGSEVYRPAKPDQRNYFRVATPDDVQGPAGGQYTYTDLGKRAALVIDDTT
                     AFGVGVADTFSTEFEALGGTIVDRQSNDYAVNTSFSAILTAAGDEFDVVYFGGTQTLG
                     GGQLRRDMGAAGLLDITMVGPDGITDLGKGGDEGTFITLAGVENSDNVHGTIAGANAL
                     ELDIAADFNAAYEAEYDVTPGAYSALAHACTSVLLQAIDANIDTAADLAALREAVRAS
                     IIGQDTPWSTVLGDISFDANGDSSQKWISFYETDPSLNDGAGGWVFVKQQDFSVTE"
     gene            1713..2645
                     /locus_tag="VMZ33_02795"
     CDS             1713..2645
                     /locus_tag="VMZ33_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUR16190.1"
                     /translation="MDERLLATLTQQLFNALAIGGIYALIALGYTMVYGIIELINFAH
                     GEIFMVGAFFAMTFLVTVGFTGTISDPLVFIVVLLGSFAVVMPVLGLVGVGIERFAYR
                     PLRNAPRLAPLITAIGVSFILQNIVYIWQGPSPVQFPQILSPQVRFSILGASVSYISV
                     LIVVVTAILLIALQLFVSRSRLGRAMRSTAQDRDAAQLMGVNINTTIALTFFLGSALA
                     GAAGIVYGLYFGTIAFNIGFIGGLKAFTAAVLGGIGNTSGAVLGGFIIGFIEVISAQL
                     GLAIWSHAIVFAVLVIVLVFRPSGLLGQPIGERA"
     gene            2642..3853
                     /locus_tag="VMZ33_02800"
     CDS             2642..3853
                     /locus_tag="VMZ33_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16191.1"
                     /translation="MTAAAAAKSAPRRSAPANARDYLGRHRSLSIIIGMSIVTAILPF
                     IVTLPPLSGFNAQRVWIDSFATAGIFVLLAIGLNVVVGLSGLLDLGYAAFYAIGAYTY
                     AFAASSFTGLHVSFWVMLLVGALVAAIFGILLGAPTLRLRGDYLAIVTLGFGEIVPIL
                     FRNADERNVGVNLTNGTNGIPGIDRPTLPVIQIGDFKILDVVFNAANPLSYYITMAVI
                     ITIVLILVYRLQDSRLGRAWQAIREDELAAASNGVNTVTTKLLAFALGATTAGFAGVF
                     SASKLVFVSPDQFGFTVSFTILAMVVLGGMGNSIGVAIGAFLIYIIQSVLLKQLTNFF
                     DNTQVPILSDINFIEYQFLLYGIALVAMMLLRPEGLFPSRQRARELHTDDGVDPMEGS
                     PSGSDAEGSLA"
     gene            3850..4956
                     /locus_tag="VMZ33_02805"
     CDS             3850..4956
                     /locus_tag="VMZ33_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16192.1"
                     /translation="MTDQAKAGSAPAADDTAAGDISGDDPKAVLAGAWSDVDESGDIM
                     LRARAVTKRFGGLVAVRDADLVIPKNKIVSLIGPNGAGKTTFFNIVAGILDPTAGDIE
                     FRGRKMIARPVRPWFESILWFIPSLVVLAVTWLLTGVVGVGALEVGVLLAVFSLVVTL
                     LLAIVRPVWYSRLLARLGVFKSARPNDMVEAGIGRTFQNIRLFQNMSAIDNVLVGMHT
                     RLKSHMIDAMLSTPRRNREERDGRKRARELLALVGLRGRDHVLARNMPYGDQRRLEVA
                     RALASNPVLLLLDEPTAGMNPAETAEMTRLISRLRAELGLTILLIEHDMRVVMGISDR
                     ITVLDHGEQIAEGTPEEVRRDPKVIEAYLGAPAS"
     gene            5007..5711
                     /locus_tag="VMZ33_02810"
     CDS             5007..5711
                     /locus_tag="VMZ33_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16193.1"
                     /translation="MLRLTNVNTYYGRIRALQGIDLEVGKGEIVTLIGANGAGKTTTL
                     RTISGLLHPREGTITFEGQDITNVPAHELVRRGIGHAPEGRRIFSRLTIHENLQMGGF
                     TRSAAERREDQERVLTLFPRLRERLTQKGGTLSGGEQQMLAIGRAMMARPRLLMLDEP
                     SLGLAPILVQQIFDIIREINSQGTTVLLVEQNAQQALAVAHRGYVLQTGTVILTDTAS
                     ALAANEDVRRVYLGEM"
     gene            5960..6229
                     /locus_tag="VMZ33_02815"
     CDS             5960..6229
                     /locus_tag="VMZ33_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16194.1"
                     /translation="MSPPAEGLLRVVLWQVDEDGRAREVVSPVITNPALFDLGEAYYA
                     PPAGEEATWPRGRYVFEVKSSTGADSGWVGIEFLAIDDEGAAALR"
     gene            complement(6226..7044)
                     /locus_tag="VMZ33_02820"
     CDS             complement(6226..7044)
                     /locus_tag="VMZ33_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HUR16195.1"
                     /translation="MPRLGDVVVSARGVEKYFGDLHVLRGVDLDVRQHETVMIIGRSG
                     SGKTTFLRCLNFLEEPSAGEIRIGDIDIKADPLHTRTREHDERVRQLRIQAGMVFQDF
                     NLFPHMSVLENCIEAPVRVRKIPRKQAIELAEQYLGKVHMLDKRNEYPARLSGGQKQR
                     AAIARALSNEPQVMMFDEPTSALDQELIGDVLHVMEQLAHEGTTMVVVTHEMHFAREA
                     ADRIVFMDEGKIIEDGPPSQILDNPQDVRTQQFLRRFRESMQVASPTYMPGWAD"
     gene            complement(7044..7895)
                     /locus_tag="VMZ33_02825"
     CDS             complement(7044..7895)
                     /locus_tag="VMZ33_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HUR16196.1"
                     /translation="MADQKASIIRTGSVVPDQPGVPQTNLVDDIRRARGRQTRRARLL
                     FVATWLAIIGGLAFALSGRFDSGFVFEWLPFILGGIGITILVSFASIIFATLFALVGA
                     LGRLSKRAPIYAASTLYVSLVRGTPLIVQVLFVYLALPQIIPAVAKIPAIYLGIFALS
                     FNYGAYMTEIFRAGIQAVSRGQTEAAQALGMTDSLLLRRIVLPQATRIVIPAIGNEFI
                     ALIKDSALVSVIAVQELLWRAQRAGSQDFKSFEAFTVAALVYWALTIIFSLFQDRLEK
                     RMARGDR"
     gene            complement(7963..8940)
                     /locus_tag="VMZ33_02830"
     CDS             complement(7963..8940)
                     /locus_tag="VMZ33_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HUR16197.1"
                     /translation="MTSPRLVVLGGALLLLAACGGGGATTRPSTAASSAPSTAASTAP
                     STAPSTAPSSAPSAAAGEAVCDNQPAEAAGDLLETICNAGELRMATDPAYPPQSELTS
                     EGEYQGFDISVGQEIADRLGVELAFETPAWEVLTAGSWQERWDISVGSMTITTEREEA
                     LDFTQPYYYNPAQLAVRDGSGITDIAGLAGKVICVGEDTTYLQWLEGTLDLGSDVQLP
                     EVPEGATATTLPTDRDCAEAWRAGREEFDGWMASAPTIADAVADGLPVIPLEPTAFNE
                     QLAAALDQSGPDHDQLLEALNSILADMHTDGTLTSFSEEWLGSDLTNPL"
     gene            9020..>9330
                     /locus_tag="VMZ33_02835"
     CDS             9020..>9330
                     /locus_tag="VMZ33_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR16198.1"
                     /translation="MDTPADLARAATLPSRYYLDAEVHELEKELIFGRTWQLVARLDD
                     LARPGDFVPATILDEPIVLTHGLDGTLRGFYNVCRHRAGQVALSKGNRKSLQCRYHGW
                     T"
BASE COUNT         1595 a         3065 c         2998 g         1672 t
ORIGIN      
        1 tgggtccggt cgccgccttg cgcgccacac cgggatggac catgcgcacg gatatcgtgc
       61 tcggccaacg ctccggggcg ctccaacgtc tggttgcaaa tgagacgttg ccgactatga
      121 tacggcatcg catcagccca ccctgtgggc ggagcggcca cgttcgtcac gcggcggcct
      181 agtgccacct tatcgacgca tcgtcgccga cgcccgtcag ccgacggcga aatgcggcgc
      241 ggatcgcttt gcgagccgca cgaggaggaa ccttttgaag gaggaaacat tcagatgaag
      301 ctgaggtatc tcggcacctt tacggcgacg atggttctcg tcgccgcctg tggtggtggt
      361 ggcaccgcta ctacgcggcc ttctacggcc gcttctaccg cggcttcggc cgctccgtca
      421 gccgctcagt cggccgcgcc gacccagggt ggcggcggca cattggacac agtcaagatc
      481 ggcgttgacc tgccgctgtc cggcgcttcg gtcgccaacg gccagccaac ccttcagggc
      541 gtcgagctgg cggtcaagga atggaacgag gccgatcatg gctacacggt cgagctcaac
      601 gtccaggacg acgcggtcaa cggcgtgtac aacccggagc agggcgccag caatgccaac
      661 accctcgtcg ccgacgaggc ggtagtgggc atggtcggtc cgttcaactc cagcgtcgcg
      721 cgggcgatca tcccagtaac caacgagttc gggctcgcgc agtgcagtcc ggccaacacc
      781 gccatcgacc tgaccaagga agggtcggag gtttaccgcc cggccaagcc tgaccagcgc
      841 aactacttcc gcgttgcaac cccggatgac gtccagggcc ctgcgggcgg gcagtacacc
      901 tacaccgacc tcggcaagcg cgccgcactg gtgatcgacg acacgacggc cttcggcgtc
      961 ggcgtcgcgg acacgttcag caccgagttc gaagcgctcg gcggcaccat cgtcgaccgc
     1021 caaagcaatg actacgcggt caacaccagc ttctccgcca ttctgaccgc ggctggcgac
     1081 gagttcgacg tcgtctactt cggcgggacc cagactttgg gcggcggcca gctgcgccgc
     1141 gacatgggcg cagccggcct gctcgacatt acgatggttg ggcctgacgg catcacggac
     1201 ctgggcaagg gcggcgacga gggtacgttc atcaccctcg ccggcgtcga gaactcggac
     1261 aacgtgcatg gcaccatcgc gggcgccaac gccctcgagc tggacatcgc ggccgatttc
     1321 aacgccgcct acgaggccga gtacgacgtg actccgggcg catatagcgc tctggcgcat
     1381 gcctgcacat cggttctcct gcaggccatc gacgccaata tcgacacagc ggcagacctc
     1441 gcggcactcc gcgaagctgt ccgcgcgtcg atcattggcc aagacacccc ctggagcact
     1501 gtgctcggcg acatctcgtt tgacgccaac ggcgactcca gccagaagtg gatctcgttc
     1561 tacgagacgg atccatcgct caatgacgga gcgggcggct gggtcttcgt caagcagcag
     1621 gacttctcgg tcaccgagta aatccggctt gaattgacag ccggggagcc gtaaaggctc
     1681 cccggctgtt ctgtatctga gcgaggacga agttggacga acggctgcta gccacgttga
     1741 cccagcagct cttcaacgcg ctggcgatcg gcggcatcta tgcactgatc gcactgggct
     1801 acacgatggt ctacggcatc atcgagctga tcaacttcgc ccacggcgag atcttcatgg
     1861 tcggcgcgtt cttcgcgatg accttcctgg taaccgtcgg gttcaccggc accatctcgg
     1921 acccgctcgt cttcatcgtc gtcttgctcg gttcgttcgc cgtcgtgatg ccggtgctcg
     1981 gcctggtcgg ggtcggcata gaacggttcg cctaccgacc gttgcgaaac gcccccaggc
     2041 tggcgccgct catcaccgcg atcggcgtca gcttcatcct ccagaacatc gtctacatct
     2101 ggcaggggcc gtcgccggtg cagttcccac agatcctcag cccgcaggtg cggttctcga
     2161 tcctcggcgc gtcagtcagc tacatcagcg tcctgatcgt cgtggtgacc gcgatcctgc
     2221 tgattgcgct gcagctgttc gtcagtcgct ctcgtctggg ccgcgcgatg cggtcgaccg
     2281 cgcaggatcg tgacgcggcg cagctgatgg gcgtcaacat caacaccacg atcgcgctga
     2341 ccttcttcct gggctcggcg ctcgccggcg ctgcgggcat cgtctacggc ctttacttcg
     2401 ggacgatcgc gttcaacatt ggcttcatcg gcggactgaa ggcgttcacg gcggcggtcc
     2461 tgggtggcat cggcaacacc agcggagcgg tgctgggcgg attcatcatc ggcttcatcg
     2521 aggtcatcag cgcacagctt ggcctggcga tctggtcgca cgcgatcgtc ttcgccgtcc
     2581 tggtgatcgt gctcgtattc cgaccctcag gcctgctcgg ccagccgatc ggagagcgcg
     2641 catgacggcc gccgctgcgg ccaagagcgc cccgcgccgc tcggcgccgg cgaatgccag
     2701 ggattacctc ggccgccacc ggtcgctctc gatcatcatc gggatgtcca tcgtgacggc
     2761 catcctgccg ttcatcgtca cgctgccgcc gctgtcgggt ttcaacgccc agcgagtgtg
     2821 gatcgactcc ttcgcgacgg ctggcatctt cgtcctgctc gcgatcgggc tgaacgtggt
     2881 cgttggccta tctggtctgc tggacctcgg ctacgccgcg ttctacgcca tcggcgcgta
     2941 cacgtacgct ttcgccgcgt ccagcttcac gggactgcac gtcagcttct gggtgatgct
     3001 cctcgtaggc gcgctcgtcg ccgcaatctt cggcatcctg ctgggcgctc cgacgctgcg
     3061 cctccgaggc gactacctcg cgatcgtcac gctcgggttc ggcgagatcg tgccgatcct
     3121 gttcaggaac gccgacgagc ggaacgtcgg tgtcaatctc acgaatggca cgaatggcat
     3181 cccgggcatc gaccgtccga ctcttccggt gatccagatc ggcgacttca agattctcga
     3241 cgtcgtgttc aatgcggcaa atccgctcag ctactacata acgatggctg tgatcatcac
     3301 cattgtcctg atcctcgttt accgcctgca ggactcgcgg cttggccgag cctggcaggc
     3361 gattcgcgag gacgagttgg ctgcggcgag caatggcgtc aataccgtta cgaccaagct
     3421 ccttgcgttc gcactgggag cgacgaccgc cggattcgcc ggcgtcttct cggcgtccaa
     3481 gctcgtcttc gtgagcccgg accagttcgg gttcaccgtc tcgttcacga tcctggcgat
     3541 ggtcgtgctg ggcggcatgg gcaatagcat cggtgtcgcg ataggcgcgt tcctgatcta
     3601 catcatccag tcggtcctcc tcaagcagct gaccaacttc ttcgataaca cccaggtccc
     3661 gatcctctcg gacatcaact tcatcgagta ccagttcctg ctgtacggca tcgccctcgt
     3721 ggcgatgatg ctcttgagac cggaagggct cttcccgagc agacagcgcg cgcgggagct
     3781 ccataccgac gacggagtcg acccgatgga gggctcgccg agcggtagcg acgctgaggg
     3841 gagcctggcg tgaccgacca agcgaaggcc ggttctgcgc cggctgctga cgacaccgcc
     3901 gcgggcgaca tttctggcga cgatcccaag gcggtcctcg ccggcgcatg gtccgacgtg
     3961 gacgagagcg gcgacatcat gctacgggcg cgagcggtca cgaagcgctt cggcggcctc
     4021 gtcgcggttc gcgacgcaga cctggtgatc cccaagaaca agatcgtcag cctgatcggg
     4081 ccgaacggcg caggcaaaac gacgttcttc aacatcgtcg ccggtatcct cgatccgacg
     4141 gcaggcgaca tcgagttccg cggccgaaag atgatcgccc ggcccgttcg gccgtggttc
     4201 gagtcaatcc tgtggtttat tccatcgctg gtggtcctcg ccgtgacgtg gctgctgacc
     4261 ggggtagtcg gcgtcggcgc gctcgaggtc ggcgtcctgc tcgcggtgtt ctcgctcgtg
     4321 gtcacgctgc tgctcgcgat cgtgcgaccg gtctggtatt cgcgactttt ggcgcgactc
     4381 ggggtgttca agagtgcgcg cccgaacgac atggtcgagg cgggcatcgg ccgcacgttc
     4441 cagaacattc gcctcttcca gaacatgtcg gcgatcgaca acgtgcttgt cggcatgcac
     4501 acccgcctga agtcgcacat gatcgacgcg atgctctcga cgccgcggcg caatcgcgag
     4561 gagcgcgacg gccgcaagcg ggcgcgtgag ctgctggcgc tggtcggcct ccgcggacgc
     4621 gaccacgtcc tggctcggaa tatgccctac ggggatcagc gccgtctcga ggtggcgcgc
     4681 gcgctggcga gcaacccggt gctgctgctg ctcgacgaac cgacggcggg catgaacccg
     4741 gctgagacgg cggagatgac acgcctgatc agccgactgc gcgccgagct cggcctgacg
     4801 atcctcctga tcgagcacga catgcgcgtc gtcatgggca tcagcgatcg catcacggtg
     4861 ctcgaccacg gcgagcagat cgccgagggc acccctgaag aggtgcgccg cgatcccaag
     4921 gtcatcgagg cctacctcgg agcgccggca tcgtgaccgc agaaacgctg ccagcgagcg
     4981 gcggtagctc cgcgcgcgac ggcgccatgt tgaggctgac gaacgtcaac acgtactacg
     5041 gccggattcg cgcgctccaa gggatcgacc tggaggtcgg gaaaggcgag atcgtcacgc
     5101 tcatcggcgc gaatggcgcg ggcaaaacga ccacgctgcg cacgatttcc ggcctgctcc
     5161 acccgcgtga gggcacgatc acgttcgagg gccaggacat caccaacgtt cctgcccatg
     5221 agctcgtgcg ccgcggcatc ggccatgcgc cggagggacg gcgaatcttc tcccgcctga
     5281 ccatccacga gaacctccag atgggcgggt tcacgcgaag cgccgccgaa cgccgggagg
     5341 accaggagcg cgtgctcaca ttgttccccc gtctcaggga gcggctgacc cagaagggcg
     5401 gcacgctgtc gggtggcgag cagcagatgc tggccatagg tcgcgcgatg atggcgcgac
     5461 cgcgtcttct catgctcgac gagccgtcgc tcgggctcgc gccgatcctg gtgcagcaga
     5521 tcttcgacat cattcgcgag atcaacagcc aggggacgac ggttctcctg gtcgagcaga
     5581 acgcacagca ggcgctggcc gttgcgcacc gcggctacgt gctgcagacg ggcaccgtga
     5641 tcctgacgga tactgcctct gccctggccg ccaacgagga cgtgcgccgt gtctacctgg
     5701 gggagatgta acccagagct cgactcggag gcgcgcaccc cgctgaaccg atggcagccg
     5761 ctctgcagcg ccggcagtgc cactcaccct caacctggcg gctggattcg tctgaggcga
     5821 ccgtaacgcg gcgtacccgg acgatgtatg ccgccgagcc ggtcgttgcc gcgggccaac
     5881 agacgaagcc gtcccgatga gccgcctcta cgctcgaacc atgcaatccg tcggcgtctg
     5941 cgttccgtgg tcatcaccta tgagcccacc tgccgaaggc ctgcttcgag tcgtcctctg
     6001 gcaggtggac gaagacggtc gggcgcgcga ggtggtctca ccggtgatca ccaatcctgc
     6061 gctcttcgat ctcggcgagg cgtactacgc gccgccagcg ggagaggagg cgacgtggcc
     6121 gcgcggccga tacgtgttcg aggtcaagag cagcaccggc gccgattcgg gttgggtggg
     6181 gattgagttt ctggcgattg acgatgaagg cgccgcggcg ctccgctagt cggcccagcc
     6241 gggcatgtac gtcgggctgg caacctgcat cgattcacgg aagcgtcgca ggaactgctg
     6301 cgtccgaaca tcctggggat tgtcgaggat ctggctcggt ggcccatcct cgatgatctt
     6361 gccctcgtcc atgaacacga tgcggtccgc ggcctcgcgg gcgaagtgca tttcgtgagt
     6421 gacgacaacc atggtcgtgc cctcgtgcgc cagctgctcc atgacgtgca gcacgtcgcc
     6481 gatgagctcc tggtcgagcg ccgaggtcgg ctcgtcgaac atcatcacct gcggctcatt
     6541 gctgagcgcg cgggcgatcg cagctcgctg cttctgtccg cctgacagcc ttgccgggta
     6601 ttcgttgcgc ttgtcgagca tgtgcacctt gcccaggtat tgctcggcca actcgatcgc
     6661 ctgtttgcgc gggatcttgc gcaccctgac cggcgcctcg atgcagttct cgagtaccga
     6721 catgtgcggg aacaggttga agtcctggaa gaccatgccc gcctgaatcc gtagctggcg
     6781 aacgcgttca tcgtgctcgc gcgtccgcgt gtgaagtggg tccgccttga tatcgatgtc
     6841 accgatgcga atttcaccgg cgcttggctc ctcgaggaag ttgaggcagc gcaggaacgt
     6901 cgtcttgcca ctccccgagc ggccgatgat catcaccgtc tcgtgctggc ggacgtcgag
     6961 atcaacgcca cgcaagacat ggaggtcacc aaagtacttt tccacgccgc gtgcgctgac
     7021 gacaacgtcg ccgagcctcg gcatcagcga tcacccctgg ccatgcgctt ctcgagccgg
     7081 tcctggaaga gcgagaagat gatggtcagc gcccagtaca cgagcgcggc gaccgtgaac
     7141 gcctcaaacg acttgaagtc ctggctaccg gctcgttggg cgcgccacag cagctcctgc
     7201 accgcgatca ccgagacgag cgccgagtcc ttgatgagcg cgatgaattc gttgccgatg
     7261 gccgggatca cgatgcgcgt cgcctgcggc agaacgatcc gacgcagcag cagtgaatcg
     7321 gtcatgccca gggcctgggc cgcttccgtc tggccgcgcg acacggcctg gatgccggcc
     7381 cggaagattt ccgtcatgta cgcgccgtag ttgaacgaca gcgcaaagat gcccaggtag
     7441 atggccggga tcttggctac ggccgggatg atttgaggca gggccaggta gacgaacagg
     7501 acctggacaa tgagcggcgt gccgcgaacg agggagacgt agagcgtcga cgcggcgtag
     7561 atgggcgccc tttttgagag ccgaccgagc gctccaacga gcgcgaagag cgttgcgaag
     7621 atgatcgacg cgaacgacac caggatcgtg atgccgatcc cgccgaggat gaacggcagc
     7681 cattcgaaaa cgaaaccgct gtcgaagcga cctgagagcg cgaaggcaag gccgccgatg
     7741 atcgccagcc acgtcgcaac gaacagcagt cgcgcgcgtc gcgtctgacg ccctcgagca
     7801 cgccggatgt cgtcgacgag gttcgtctgg ggcacgcccg gctgatccgg gacgacactg
     7861 ccggtcctga tgatcgacgc cttttgatcg gccacttacc cgccagcaag agtgaggccc
     7921 cgggcgaagc cgtccggggc catcggcgcg ctaggttacg acctagagcg ggttggtcaa
     7981 gtcgcttccc agccactcct cggagaagct cgtgagagtc ccgtcggtgt gcatgtcggc
     8041 gaggatcgag ttgagcgcct cgaggagctg gtcgtggtcc ggcccgctct ggtcgagcgc
     8101 cgcggcaagc tgctcgttga aggctgtcgg ctcaagcggg atcacgggca agccgtccgc
     8161 caccgcgtca gcgatcgtag gtgccgatgc catccagccg tcgaattcct cccgtccggc
     8221 ccgccacgcc tcggcgcagt cccggtccgt cgggagagtt gttgctgtcg cgccttccgg
     8281 cacttctggc aactgcacgt cagagccaag gtcgagggtg ccctccagcc actgcaggta
     8341 ggtcgtgtcc tcgccgacgc agatcacttt gcccgccaga ccggcgatgt cggtgatgcc
     8401 gctcccgtca cgtacggcca gctgagccgg gttgtagtaa tacggctgtg tgaagtcgag
     8461 agcctcctcg cgctccgtcg taatagtcat cgagccgacg ctgatgtccc atcgctcctg
     8521 ccagctgccg gccgtcaaga cctcccatgc gggcgtttcg aacgcaagct cgacgccgag
     8581 ccggtccgca atctcctggc cgacgctgat gtcgaagccc tgatattcgc cctccgaggt
     8641 cagctcggac tgcgggggat aggcaggatc ggttgccatc cggagctcgc ctgcgttgca
     8701 gatggtctcg agcaggtcgc ctgcggcctc cgccggctgg ttgtcgcaga cggcttctcc
     8761 cgcggcggcg gagggcgcgc tactcggcgc cgtggatggt gcggtcgatg gcgccgtcga
     8821 ggccgcggtt gacggagcgc tggacgcggc ggtcgatgga cgcgtcgtcg cgccgccgcc
     8881 accacacgcg gcaaggagca gcagcgcccc tccgagaaca accagacgag gtgaagtcat
     8941 ttgcaaaatc ctcctgaaaa tggctcatac actctagacg atggctaccg gcgttcggag
     9001 tgaatattcc agttccggag tggatactcc ggctgacctt gcgcgggccg cgacgctgcc
     9061 ttcccgttat tacctggacg ccgaggtcca cgagctcgag aaggagctca tcttcggacg
     9121 cacctggcag ctcgtcgctc gacttgacga cctcgcccga ccgggcgact tcgttccggc
     9181 cacgatcctc gacgagccga tcgtcctgac tcacggcctc gatggcacgt tgcgcggttt
     9241 ctacaacgtt tgtcgacacc gcgccgggca agttgccctt tctaaaggaa atcgcaagag
     9301 tttgcagtgt cggtaccacg ggtggaccta
//
LOCUS       DATLCO010000115         5356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5353607, whole genome shotgun sequence.
ACCESSION   DATLCO010000115 DATLCO010000000
VERSION     DATLCO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5353607"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..394
                     /gene="rpsI"
                     /locus_tag="VMZ33_02840"
     CDS             2..394
                     /gene="rpsI"
                     /locus_tag="VMZ33_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HUR16199.1"
                     /translation="MATTNLITAIGRRKTAVARVLLQQGNGQITVNGKPLNAALPRAT
                     LQYIATQPFRAVGGARFSVRVKVTGGGISGQAGAISHGLARALVKADETMRPLLRKAG
                     LLTRDARMKERKKYGLKRARKAPQYTKR"
     gene            482..1225
                     /locus_tag="VMZ33_02845"
     CDS             482..1225
                     /locus_tag="VMZ33_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR16200.1"
                     /translation="MTDDVYARYREALRLGHQEASEGHFAQALTLYQTAADLATGRAL
                     PLVGVGSMNLRLGRARDALVAYERALSFEPTNLDALTGRAAALLAAGRRDEAARVQQQ
                     IIDIRDGGPPAAAPAFNATPTSVADALYMEGDQALRRHNNDAAIDAWLGESREHAGAT
                     RFDAALDATMRALAIDPSSARVHLEMARLYFARGWVEQGVERALLLDRLLALEPDPRI
                     QLALRSLAAANVESDDRLATLAGAHAGAE"
     gene            1259..2272
                     /gene="cdaA"
                     /locus_tag="VMZ33_02850"
     CDS             1259..2272
                     /gene="cdaA"
                     /locus_tag="VMZ33_02850"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HUR16201.1"
                     /translation="MPDQLVELIRGFEVQPVAFLDIALTALLIYGLLSLIRGTRAVRL
                     VIGVSILYAVYVAAQILGLELLSQILQAAAVVGLLALVVVFQPELRRGLERIGRVGSL
                     GWLIAPATRREYDRVSRVIAQAAATLSSQKTGALIVVERETGLTDTAESGVPIDAKLS
                     PELLETIFTPRSALHDGAVIIRHDRMVSAGVVLPLSESGSLRERFGTRHRAALGISEQ
                     TDAVVVVVSEETGQIALVERGRIVRNMDEERLRRALFDILEHNTALSAARIPARANAL
                     RRNLPIRRPRRDTVATEGSSAGAGTAASAPNSPATASAPAAGAGASAAATTPGPTPSG
                     RFE"
     gene            2269..3525
                     /locus_tag="VMZ33_02855"
     CDS             2269..3525
                     /locus_tag="VMZ33_02855"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HUR16202.1"
                     /translation="MRRALDFLVRNWPLKVGAIFLATILYSGLVLGQNVRTFNGTVPV
                     EALRPPLNATLLSDLDPITQVRFRAPLDIGVISPTSFVATVDLAPVEPEPGGPTVDVP
                     VTLTAIDQRITIVDYQPRTVAIRLDPVGSRELPITVSIVSAPDGLDLGPPQTDPSSVT
                     IRGASSRVDAVTQVLARVSVDASGLNIDRDFDLVPIDANGNQVPNVELEPERARIRIA
                     VARQLANRTLPVLPQIVGTPAAGYRIASVTVEPLVVTVSGEENIVTQMQTATTEVIDI
                     NGRTDDLEASVRVAVPAGASVSGTDTVRVLIVIDEDIGTRTLQIGVQMAGERSGFAYV
                     PRTDRVAVTLGGPIAVLDAIVPSQLVALLDMASYATAGGEFNVPVEIEPPPGLEIVTI
                     DPPEVAVFIAALPTSPSTVTPPSPTP"
     gene            3522..4856
                     /locus_tag="VMZ33_02860"
     CDS             3522..4856
                     /locus_tag="VMZ33_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16203.1"
                     /translation="MTRLFGTDGIRGVANVDLTPRLAYELGRATAAKITGGVGGHLLI
                     GQDTRRSGDMLAAAVAAGAMSMGTDVHRLGVCPTPALAHITASGSFGAGIMVSASHNP
                     ARDNGLKVFDGKGIKLEEALEDELEALMPRVDGLPDPENDGIGVDLDARSRLSEYLDH
                     RLEIASRFKCDARVLIDCANGSGGVVALQILAGTGANVEAIFNAPDGININLECGATA
                     PTAAANAVKQTSANVGFALDGDADRCVAIDERGEVVDGDQLIGIIALDRLQRGTLGNG
                     LCVVSVLSNGGLEQAINAAGGRVARTAVGDKYILDGMIVMDAALGGEKSGHIIVREYA
                     QAGDGIVTALEVLSILASTGKPLSALAAQIPLYPQQQRTIPVRHKDQWEADPAFGQAL
                     DEARRSVDGRGRILVRPSGTEPALRIMVEGDSHDDVLRIADHLAALAGERLN"
     gene            4917..>5356
                     /locus_tag="VMZ33_02865"
     CDS             4917..>5356
                     /locus_tag="VMZ33_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16204.1"
                     /translation="MCGIVGYIGPRDAAPILLEGLARLEYRGYDSAGIALVTTNGEMF
                     VEKRAGKLANLRTALIDGAPSAAVGLAHTRWATHGRPNDMNAHPHSDCSGQITVIHNG
                     IIENFKALRDGLRERGHKLQSDTDTEALAHLIEEAYVDMGDIGDA"
BASE COUNT          894 a         1837 c         1780 g          845 t
ORIGIN      
        1 catggcaacg accaatctga tcacggcgat cggccggcgc aagaccgcgg tcgcgcgcgt
       61 cctgctgcag cagggcaacg ggcagatcac cgtcaacggc aagccgctca acgcggcgct
      121 gccgcgcgcg accctgcagt acatcgccac ccagccgttc cgcgccgtcg gcggcgcgcg
      181 cttcagcgtg cgcgtcaagg tcaccggcgg tgggatctcc ggccaggctg gcgcgatctc
      241 gcacgggctc gcgcgggcgc tggtcaaggc cgacgagacg atgcggccgc tgctgcgcaa
      301 ggccggcctc ctcacgcgcg acgcgcgcat gaaggagcgc aagaagtacg gcctcaagcg
      361 ggcacgcaaa gcgcctcagt acacgaagag atagtgcttc gcagcagccc tcacgggcac
      421 tgctgcgcgt ggcctccttg cgggcgctag cctcggcgcg acgaccggtc gccgagcgca
      481 catgaccgac gacgtttacg cccggtatcg cgaggcgctg cgtctcggcc accaggaagc
      541 ttctgagggg cacttcgcgc aagcgttgac cctctaccaa accgctgcgg atttggcgac
      601 cggccgcgcc ttgccgctcg tcggcgtggg cagcatgaac ctgcggctgg ggcgcgccag
      661 ggacgcactc gtggcgtacg aacgggcact gagcttcgaa ccgaccaacc tcgacgcgtt
      721 gaccggtcgc gcggcggcgt tgttggcggc cgggcggcgc gatgaggcag cccgcgtaca
      781 acagcagatc atcgacatcc gggatggcgg tccgccggcc gccgcgccgg cattcaacgc
      841 gacgcccacg tctgttgccg acgcgctgta catggagggc gaccaggcac tgcgccgtca
      901 caacaacgac gcggcaatcg acgcgtggct gggtgagagc cgcgagcacg ccggcgccac
      961 acgattcgac gcggcgcttg acgccacgat gcgggccctt gccatcgacc ccagctcggc
     1021 gcgcgtccac ctcgagatgg cgcggctgta ctttgctcgc ggttgggtcg aacagggtgt
     1081 cgaacgggca ttgttgctcg atcgactact ggctcttgag cccgaccccc gcatccagct
     1141 cgccttgcgc tcattggccg ccgccaacgt tgagagcgac gataggctgg cgacgctcgc
     1201 tggggcccac gctggtgctg agtaacttgc ccagccgccc gacgtacaat cggccgcaat
     1261 gccggaccag ctggtcgaac tgatcagggg ctttgaggtc cagccggtcg cgttcctcga
     1321 tatcgcgctc acggctctcc tgatctacgg gctgctcagc ctgatccgcg gcacgcgcgc
     1381 cgtccgcctc gtgatcggcg tttcgatcct ttatgcggtc tacgtcgcgg cacagatcct
     1441 cggcctggag ctcctgtctc agatcctcca ggcagccgcg gtcgtcggcc tcctggcgct
     1501 cgtggtcgtg ttccagcccg agctgcgtcg cggccttgaa cgaatcgggc gcgtcggttc
     1561 gcttggctgg ctgattgctc cggccacgcg acgtgaatac gaccgcgtat cgcgcgtgat
     1621 cgcacaggcg gccgcgaccc tttcatccca gaagactggc gcactcatcg tcgtggaacg
     1681 cgaaaccggc ctgaccgaca ctgcagagag tggcgtgccc atcgatgcca agctctcgcc
     1741 cgagctgctc gagacgatct tcacgccgcg ctccgcactg cacgacggcg cggtgatcat
     1801 ccgccacgac cggatggtgt cggccggagt cgtcctgcca ctgtccgagt cgggctcgtt
     1861 acgcgagcgc ttcggtacgc gccatcgcgc agcgctgggg atcagcgagc agactgatgc
     1921 ggtcgtcgtc gtggtcagcg aggaaaccgg gcagattgcc cttgttgagc gcggccgcat
     1981 cgtccggaac atggacgagg agcgtctccg tcgagccctc tttgacatcc tggagcacaa
     2041 caccgccctg tcggccgcgc gcatcccggc ccgcgctaat gcgctgcgcc ggaacctgcc
     2101 gattcgccgt ccgcgccgcg acacggtagc cacggagggc tcgagcgcgg gcgccggcac
     2161 agcggcaagc gcaccgaatt ctccagccac cgcgtcggcg ccggctgctg gtgcgggggc
     2221 atcggcggcg gccacgacac cggggcctac cccgagtggc aggttcgagt gaggcgcgcc
     2281 ctcgacttcc tggtccgcaa ctggccgctg aaagtcgggg ctattttcct cgccacgatc
     2341 ctctacagcg gcctggtact cggccagaac gtccgcacgt tcaacggtac ggtcccggtt
     2401 gaggcgctgc ggccaccgct caacgcgacg cttctatccg atctcgatcc gatcacacag
     2461 gtgaggtttc gggccccgct cgatatcggt gtcatctcgc cgacttcttt cgtggcgacg
     2521 gtggatctgg caccggtcga acccgagcca ggcgggccaa ccgtggacgt gcccgtgacg
     2581 ttgacggcca tcgaccagcg aatcacgatc gtcgactacc agccacgtac cgtcgccatc
     2641 cgcctggacc ccgtggggtc acgtgaattg ccaatcacgg tctcgatcgt gagcgcgcct
     2701 gacggcctcg accttggacc gccacaaacc gatccgagct ccgtcacgat ccgcggtgcg
     2761 agctcgcgtg tggacgcggt cacccaagtc ctggcgcgag tctccgtcga cgcaagcggc
     2821 ctcaacatcg atcgcgactt cgacctcgtg ccgatcgacg ccaacggcaa ccaggtgccg
     2881 aatgtcgagc ttgagcccga gcgggcgcgc atccgcatcg cggtcgcgcg gcagctggcc
     2941 aatcgcacgc tccccgtcct gccgcagatc gtggggacgc cggcggccgg ttatcgcatc
     3001 gcgtccgtga ctgtcgaacc gctcgttgta accgtgagcg gcgaagagaa catcgttacc
     3061 cagatgcaaa cggccacgac cgaagtcatc gacatcaacg ggcgcacaga tgacctggag
     3121 gcgagcgttc gcgtagctgt cccagccggc gccagcgtga gcggcacgga tacggtccgc
     3181 gtgctgatcg tgatcgacga ggacatcggc acccgaacct tgcagatcgg agtgcagatg
     3241 gccggcgaac ggtcaggatt tgcttacgtg ccgcgcaccg atcgcgtggc ggtgacactc
     3301 ggcgggccga tcgcggtcct ggacgctatc gtgcccagcc agctggtggc attgcttgat
     3361 atggcgtcct acgcgactgc cggcggcgaa ttcaacgttc ccgtcgagat cgagccgcca
     3421 ccggggctcg agatcgtgac aatcgatcct ccggaggttg cagtcttcat cgccgcactg
     3481 cccaccagtc cttcgacggt gacaccgccg tcgccgacgc cgtgacgcga ctctttggca
     3541 ctgacggcat acgcggcgtc gccaacgttg acctcacacc acggcttgcg tacgagctcg
     3601 gccgtgcgac cgcggccaag atcacgggtg gcgtcggcgg ccacctgctg atcgggcagg
     3661 acacacgtcg atcaggcgac atgttggccg cggctgtcgc cgccggcgcc atgtcgatgg
     3721 gcacggacgt tcatcggctg ggcgtctgtc caacaccggc attggcgcac atcacggcgt
     3781 ccggcagctt cggcgccggg atcatggtct cggccagcca caacccagcg cgcgacaacg
     3841 gcctgaaggt gttcgacggc aagggcatca agctcgagga ggccctcgag gacgagctcg
     3901 aggcgctgat gccgcgcgtc gacgggctgc ccgaccctga gaacgacggg atcggcgtcg
     3961 atctggacgc gcgatcgcgc ctcagtgagt acctcgatca ccggctcgag attgcgagtc
     4021 ggttcaagtg cgacgctcgc gtgctgatcg actgcgcgaa cggctcgggt ggtgtcgtgg
     4081 cgctgcagat cctcgctgga actggagcta acgtcgaagc gatcttcaac gcacccgacg
     4141 gcatcaacat caacctcgag tgcggcgcca cggcacctac tgccgcagct aacgccgtca
     4201 agcaaacgag tgccaacgtt gggttcgcgc tcgacggcga cgccgaccgt tgcgtggcca
     4261 tcgacgagcg tggcgaggtc gtcgatggtg accagctgat cggcatcatc gcgctcgacc
     4321 ggctccaacg cggcacgctc ggcaacgggc tgtgcgtcgt cagcgtgctg tccaacggcg
     4381 gcctggagca ggccataaac gcggccggcg gacgggtggc cagaaccgcc gtcggtgaca
     4441 agtacatcct ggacgggatg atcgtcatgg acgcggcact cggcggcgag aagagcggtc
     4501 acatcatcgt gcgcgagtac gcccaggcgg gcgacgggat cgtgacggcg ctggaggtgc
     4561 tgtcgatcct cgccagcacc ggcaagccgc tgtctgcgct cgcggcgcag atcccgcttt
     4621 atccgcagca gcagcggacg attcccgttc gtcacaagga tcagtgggag gctgacccgg
     4681 ccttcggcca ggcgctcgac gaggcgcgtc ggtcagtgga cggacgcggc cgcatcctcg
     4741 ttcgaccgtc aggtacggag ccggcgctcc gaatcatggt cgagggcgat agccacgacg
     4801 atgtgctccg gatcgccgac cacctcgctg cgctagccgg cgagcgacta aactgaccgg
     4861 ccctgcccgc tggcgcgagc ggtcggggag tggcgtggaa ggatcgggct aggcctatgt
     4921 gtggaatcgt cggttacatc ggcccgcgtg atgcggcgcc cattcttctc gagggcctcg
     4981 ctcggctcga gtaccgcggc tacgactccg cggggatcgc cctggtgacg acgaatggcg
     5041 agatgttcgt ggagaagcgc gccggaaagc tggccaatct gcgcaccgcg ctcatcgacg
     5101 gcgcgccctc cgccgccgtc ggcctggcac acactcgctg ggcaacgcac ggccggccga
     5161 acgatatgaa cgcccacccg cacagcgact gctcggggca gatcacggtc attcataacg
     5221 ggatcatcga gaacttcaag gcgctgcgcg acggcctgcg cgagcgcgga cacaagctcc
     5281 agtccgacac cgacaccgag gccctcgcgc acctcatcga agaggcgtac gtcgacatgg
     5341 gcgacatcgg cgacgc
//
LOCUS       DATLCO010000116         4788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1628070, whole genome shotgun sequence.
ACCESSION   DATLCO010000116 DATLCO010000000
VERSION     DATLCO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628070"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VMZ33_02870"
     CDS             <1..304
                     /locus_tag="VMZ33_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HUR16205.1"
                     /translation="LLGHEAIAGMLMVGTISGVLLATVLNNGGGAWDNAKKYIESGHL
                     SDDDGNVIGKGTPAHAAAVVGDTVGDPFKDTAGPSLHVLVKLLATITLVLAPLFIR"
     gene            438..1265
                     /gene="uppP"
                     /locus_tag="VMZ33_02875"
     CDS             438..1265
                     /gene="uppP"
                     /locus_tag="VMZ33_02875"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HUR16206.1"
                     /translation="MDTLIQAIVIGIVQGLTEFLPVSSSAHLILLPRVLGWDDEFLLS
                     PEFTVMLHVGTLAALIIYFWRDLIRYAAAFLRVLRDRRVGDDPERRVSVLLAASVIPG
                     ALVGVALESFIDSFFREQLLVVCALLVVGAVILFAAERLAAHTRGMADLKLTDALIIG
                     VAQALALFPGISRSGITISAGLFLGLKREQAARFAFLMGTPIIAGAALWKARELLDGS
                     AGAFDATVLAAGVIASGVSGLIAIGVLLSYLRRASTDVFGVYRIIAALVFAALILIR"
     gene            1324..1797
                     /gene="argR"
                     /locus_tag="VMZ33_02880"
     CDS             1324..1797
                     /gene="argR"
                     /locus_tag="VMZ33_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HUR16207.1"
                     /translation="MEVIKRLRQQQIRDLVSSQTIGTQHELAASLRERGYRVTQATIS
                     RDVTEMGLIKHSANGSRGYALPPVVEQPETTGEERLALVLRNLPVQIRHAGNLLVVRA
                     VPGSAHPIAAALDRTGWPEVIGSIAGDDTVFVACADRASADRISRRLNQLSRLQT"
     gene            complement(1805..3094)
                     /locus_tag="VMZ33_02885"
     CDS             complement(1805..3094)
                     /locus_tag="VMZ33_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16208.1"
                     /translation="MLSHLDRPGRAAAVVALAAILASAIGVSAGEGPPANDGFDQAAN
                     VPPSALPYSTTTSSLSATNEGGEPVTKCGATTSSIWFRFVPSSTGVYRVDTTGSNYDT
                     QVDVFRGTALNNLMLVDCNDDIDATNPDLRVSRLAFRGVAGQQLYFRVAASGPSGFTV
                     VLALRKVIAPGNDSYGQATTISSLPYDTNANNTNATIQTGEPGASTPCQTQGATRWYR
                     YTPQVDEVIWAHTSGTPDFDTVLGVYTGGTVGAAALVLCNDNQYLSGEMVNASGVTFR
                     AKANVTYRFQVGGLRGQSGDYSELQFHVRRLASVEHNDAFANAMPINGFQFSDPVTLR
                     KSTLQPDEPNCYGSNSVWYKFTPNSGANLQVATSDTFNSRVGVFESTGPGITNLQMKL
                     CGSTGSYPFEAGKTYYIVVVGEGSRAPAVTFQMEPAT"
     gene            3270..3734
                     /gene="greA"
                     /locus_tag="VMZ33_02890"
     CDS             3270..3734
                     /gene="greA"
                     /locus_tag="VMZ33_02890"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HUR16209.1"
                     /translation="MNNKPAYLSKEGLEQLREELEQLVNVKRAEVASRIHEAKEHGDI
                     SENAEYEDAKNEQAFVEGRIQSLSALIKNAVVIAEKHSTTHVQIGSTVDLQSSDGKES
                     FTIVGSTEAAPAQGRISNESPVGKALLGRKKGDTIVVTVPAGDQTYKILSIR"
     gene            3745..>4788
                     /gene="lysS"
                     /locus_tag="VMZ33_02895"
     CDS             3745..>4788
                     /gene="lysS"
                     /locus_tag="VMZ33_02895"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HUR16210.1"
                     /translation="MQPVADRSPQAYWADEIAGQVVGRQVVNDSKTPSGTVHVGSLRG
                     VVLHDAVRRVVAARGMDVEFRYGIEDLDPMDSQALLTPDAVERYMGVPLSHVPAPDGS
                     PHPNYARHFATIFLETFAELGIHPHLYWMSEQYGSGAMDPYIVKALDHAEVILDIYRT
                     VSTVNHPSGWLPISTICENCGKIGTTQSRDWDGREVSYSCRADLVEWARGCGHEGRLA
                     PFGGRAKLVWNVDWAARWGITGATIEGCGKDLATAGGSRDRADAIARRVFGYEPPLNF
                     PYEFLNLGGRKMSTSKGVGAAAHEVAALLPPTIMRFLFLRYRPNHAINFDPTGDSVPR
                     LFDEYDRIADAVAG"
BASE COUNT          820 a         1562 c         1525 g          881 t
ORIGIN      
        1 gctcctcggc cacgaggcca tcgccggcat gctcatggtc gggacgatct cgggcgttct
       61 gctcgcgacc gtgttgaaca acggcggtgg cgcctgggac aacgccaaga agtacatcga
      121 gtcgggtcat ctgtcggatg acgacggcaa cgtgatcggc aagggcacgc ctgcccatgc
      181 cgccgccgtt gtcggtgaca cggtcggcga ccccttcaag gacaccgccg gcccgtcgct
      241 ccacgtcctc gtgaagctgc tggctacgat caccctggtg ctcgcgccgc tcttcatccg
      301 ctagctcgga ccggcagcgc cgtcgttcgg ctgctgggtt ccctcaaatg gacgcaactg
      361 aattccgcgg gatcggcgta agccggttcc gcggtctgat tccagacacc ccgcggccgc
      421 tcggccccga tacctagatg gacacgctga tccaggcgat cgtcatcggg atcgttcagg
      481 ggctgactga gttcctgccc gtctcgtcgt cggcgcatct catcctgctg cctcgggtcc
      541 tcggctggga tgacgagttc ctcctgagcc cggaattcac cgtcatgctc cacgttggga
      601 ctctcgccgc gctcatcatc tacttctggc gcgacctaat ccgctatgcc gccgcgttcc
      661 tccgcgtcct gcgcgatcgt cgggtgggcg acgatcctga gcggcgcgtc agcgtcctcc
      721 tggcagcgag cgtcattccc ggcgccctcg tcggagtcgc ccttgaaagc ttcatcgact
      781 ccttcttccg cgagcagctc ctcgttgtgt gtgcgctgct cgtcgtcggt gccgtcatcc
      841 tgttcgcggc ggagcggctc gccgcccaca cccggggcat ggccgatctc aagctcaccg
      901 atgccctgat catcggcgtc gcgcaggcgc tggcgctctt cccgggaatc agccgttcag
      961 gaatcaccat ctcggcgggg ctgttccttg gcctgaagcg cgagcaggcc gcacggtttg
     1021 cgttcctgat gggcaccccg atcattgccg gtgccgccct ctggaaggcg cgcgagctgc
     1081 tggacggcag cgccggcgca ttcgatgcca cggttctggc cgccggcgtg atcgcgtccg
     1141 gggtgtcggg cctgatagcg atcggcgtcc tgctctcata cctccgtcgc gcctcgacgg
     1201 atgtctttgg cgtgtaccgc atcatcgcgg cactggtttt cgcggcactg attctcatcc
     1261 gctgacggtt cgtgcgcgcg ggcgcacaat cggcgcatac atatgcaagc gacccactaa
     1321 accgtggaag taatcaagcg cctccgccag cagcagatcc gcgacctcgt ctcgtctcag
     1381 acgatcggga cccagcatga gctcgcagcg tccctgcgcg agcgcgggta ccgagtgacg
     1441 caggcgacga tcagccgcga cgtcacggag atgggcctta tcaagcattc ggccaatggg
     1501 tcgcgcggct acgccctgcc gccggtcgtg gagcagcctg agaccacggg ggaggaacgc
     1561 ctcgccctgg tgctccgcaa tttgccggta cagatccgac acgcaggcaa cctccttgtg
     1621 gttcgcgcgg tgcccggttc ggcgcatccg atagccgccg cgctcgatcg gacgggttgg
     1681 ccagaggtga tcggatcgat agccggcgac gacacggtct tcgtggcctg tgcagatcgc
     1741 gcatcggccg accggatcag ccgccggcta aaccagcttt cgcgattgca gacttaggcc
     1801 ggcttcaggt agcgggctcc atctggaacg taaccgccgg ggcccgagat ccttctccca
     1861 cgacaacgat gtagtaggtc ttgccggcct caaacgggta tgagccggtg ctcccgcaaa
     1921 gtttcatctg gagattggtg ataccgggcc ccgtcgactc gaagacgccc accctcgaat
     1981 tgaacgtgtc tgaggtcgcc acttggagat tggctccgga gttgggcgtg aatttgtacc
     2041 agacgctgtt ggacccatag cagttgggct cgtccggctg gagcgtcgac ttgcgcagcg
     2101 tcaccgggtc tgagaattgg aacccgttga tgggcatggc attggcgaat gcgtcgttgt
     2161 gttcgacaga ggccaagcgc cgcacgtgaa actggagctc cgagtagtcg ccactctgac
     2221 cgcgcagccc accgacctgg aaccgatacg tcacgttggc cttcgcccgg aatgtgacac
     2281 ccgacgcgtt caccatctcg ccgctgagat attggttgtc attgcacagc accagggccg
     2341 ccgcgccgac tgttccaccc gtgtacacac ccagcacggt gtcgaagtct ggcgtgcccg
     2401 aggtgtgcgc ccagatcacc tcgtcaacct ggggtgtgta tcgataccag cgcgtcgcgc
     2461 cctgcgtttg gcaaggtgtg gatgcccccg gctcaccggt ctgaatggtc gcgttggtgt
     2521 tgttcgcgtt cgtgtcgtag gggagggatg agatcgtcgt cgcctggccg taggaatcgt
     2581 tgcccggcgc aatcaccttc cgcagcgcca acacaacggt gaagccactt gggcccgacg
     2641 ctgcgacacg aaagtagagc tgttgtccgg caactccacg gaaggccaac cgcgaaacgc
     2701 gcaagtctgg gttggtggca tcgatgtcgt cgttgcaatc gacgagcatg aggttgttca
     2761 aggccgttcc acggaacacg tcaacctggg tgtcgtagtt gctgccggtc gtgtcgacgc
     2821 ggtaaacgcc tgttgacgac ggcacgaacc gaaaccagat cgaggaagta gtcgcgccgc
     2881 acttcgtcac cggctcgccg ccctcgttcg tcgcgctcag cgacgaggtc gtcgtcgagt
     2941 acggcaatgc gctcggcggg acgttcgccg cctggtcaaa gccgtcgttt gctggcgggc
     3001 cctctccggc tgatacgccg atggccgacg cgaggatggc ggccagtgcc acaacggctg
     3061 cggcccggcc cggacggtcc agatgactga gcacggtgag tgatcccctt ttcaaaacgg
     3121 gaggttggtc gcgcgattat tccagacgcc tccaaccgag ggcagatggc gccctgacgc
     3181 tataatccgc cgccggacca cgaactctcg gcgcgcccgg actgtcacgt cccgccgggg
     3241 gtctttcgtt ttagatggac taattactga tgaacaacaa gcccgcatac ctgtccaagg
     3301 aaggcctcga gcagctgcgt gaggagctgg agcagctcgt caacgtcaag cgcgccgagg
     3361 ttgcctcgcg catccatgag gccaaggaac acggtgacat cagcgagaac gccgagtacg
     3421 aagacgccaa gaacgagcag gcgttcgtcg aaggtcgtat ccaatcgctg tcggcgttga
     3481 tcaagaacgc agtcgtcatc gccgaaaagc actcgacgac gcatgtccag atcggatcga
     3541 ccgtcgatct gcagagctca gacggcaagg aaagcttcac gatcgtgggc tcgaccgagg
     3601 ccgcgccggc acaggggcgc atctcgaacg agtcgccggt cggcaaagcg ctgctcgggc
     3661 gcaagaaggg cgacaccatc gtggtcacag tgcccgctgg cgaccagacc tacaagatcc
     3721 tcagcattcg ctagggagcg gcacgtgcag ccggtagcag accggtcgcc gcaggcctac
     3781 tgggccgacg agatagccgg ccaggtagtc ggtcggcagg tggtcaacga ctccaagacg
     3841 ccgagtggca cggtccacgt cggatcgttg cgtggcgtgg tgcttcatga cgcagtccga
     3901 cgtgtcgtcg ccgcgcgcgg gatggacgtc gagtttcgct acgggatcga ggatctcgat
     3961 cccatggact cccaggcgct cctgacccca gatgcggtcg agcgctacat gggcgtgccg
     4021 ctctcacatg tgccggcgcc ggatgggtcg ccacatccca actatgcgcg ccacttcgcg
     4081 acgatcttcc tcgagacgtt cgcagagctg ggtatccacc cgcacctgta ctggatgagc
     4141 gagcagtacg ggtcgggggc catggatccc tacatcgtca aggcgctcga ccatgccgag
     4201 gtcatcctgg acatctaccg cacggtctcg accgtcaacc acccatccgg ctggctgccg
     4261 atcagcacca tctgcgagaa ctgcggcaag atcggcacga cgcagtcccg tgactgggac
     4321 ggtcgcgagg tttcgtactc gtgccgcgct gacctcgttg agtgggcgcg cggctgcggc
     4381 catgagggac gcctcgcccc tttcggcggc cgtgccaagc tcgtatggaa cgtcgactgg
     4441 gccgcccgct gggggataac cggcgcaacg atcgagggct gcggtaagga cctggcgacg
     4501 gccggcgggt cacgcgaccg cgctgacgcg atcgcccgac gagttttcgg ctacgagccg
     4561 ccgctgaact tcccgtacga gtttttgaac ctgggtggcc gaaagatgag cacatccaag
     4621 ggtgtcggcg ctgcggcgca tgaggtcgcc gccctcctgc cgccgacgat catgcgcttc
     4681 ctgttcctgc gctatcgacc gaaccacgcg atcaacttcg atccgaccgg cgactccgtg
     4741 ccccgcctct tcgatgagta cgaccggata gccgatgccg tagccggt
//
LOCUS       DATLCO010000117         4187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8265155, whole genome shotgun sequence.
ACCESSION   DATLCO010000117 DATLCO010000000
VERSION     DATLCO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8265155"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VMZ33_02900"
     CDS             complement(<1..704)
                     /locus_tag="VMZ33_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16211.1"
                     /translation="MTRREQTRMMARPIARESDGMTARRAIGPIAIVGVLALVGAVAL
                     STLLGGGGGAGSASPSPGGAAANGASQRPQGTVGGGGQGAGAGRTPRPSGATPTPRQT
                     ERPPRATPTPQPSTDPQPTPVGGRAPTSPAEFDLRGQVIDIGFPLRTDANYQYRDNWL
                     DVRTGAPEDYNHSRLRDGTVERLHDGIDIYAGLGQPLLAPFSGVVMDPRSRWQPWEQR
                     QRYGRTVVIVSDEPTS"
     gene            complement(701..964)
                     /locus_tag="VMZ33_02905"
     CDS             complement(701..964)
                     /locus_tag="VMZ33_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16212.1"
                     /translation="MLSLRVAIALAMAIVAFSAAPSLAADPLNQLGPTPTPVPVFVDP
                     LDPRAGAGANRVGAPLLALVAVIGVGVAAAAVTLVYIRVTRPR"
     gene            complement(1000..1725)
                     /locus_tag="VMZ33_02910"
     CDS             complement(1000..1725)
                     /locus_tag="VMZ33_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16213.1"
                     /translation="MSPLAVALLSFGTGSLLVGFLLVRSVGRGARIGRLLAAARNVPI
                     ADARKLAADGAPAYVRVEGRITSDEEFPDDHERPLVFRRTRIEAQGDGRWTSLMDERE
                     AVPFALETRSESIAIDEAALLDGLVVIPRESVGTVADLPDDLKADVAATVTPDTPARL
                     TIHQLSAVEHATACGVPVLRDGEPTLTAGLGRPLIVTSLDQNAAMRVLAGERRGRLVV
                     GTLAAIGGFLMVVAGIVIRLLIG"
     gene            complement(1945..2505)
                     /locus_tag="VMZ33_02915"
     CDS             complement(1945..2505)
                     /locus_tag="VMZ33_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16214.1"
                     /translation="MEPGRLAVQYPGGISARFRWGGPGQADVVFGVSESAGELNDYTE
                     LSGSGASACRAELRVESPSGSWTARLASQVFDEPQGTLVDTANLLLIKYGFAVYAFHA
                     RSGELAWSHMSGTPVLSVLASTRLDHVLMQTELETLALRNDGEVAWRAAHSDVITHAQ
                     LVAGRLDLTTYNGLHVYLDATTGQAA"
     gene            complement(2513..3052)
                     /locus_tag="VMZ33_02920"
     CDS             complement(2513..3052)
                     /locus_tag="VMZ33_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HUR16215.1"
                     /translation="MTVTPDATQRVLDAAERKGIKLDVVFFDESTRTAEEAARAVGTE
                     VGAIVKSLIFVSEHVDGLEAFLVLASGANQVDVALLGAVLTEPRLRRANASEVRELTG
                     FAIGGIPPFGHKQVLRTIMDPDLGRHEQVWAAAGTPNAVFPIAPQTLRLLSNAVVTSI
                     TGGFLPTEAERTASAPTNA"
     regulatory      3130..3237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3269..3970
                     /locus_tag="VMZ33_02925"
     CDS             3269..3970
                     /locus_tag="VMZ33_02925"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HUR16216.1"
                     /translation="MRAIVVADGEIRGAAQAEPLAEWLAARKAGELMLVVAADGGAAK
                     ADLLGLPVDLVVGDADSLTEDAFRRLAAEGVEVNRYPADKNESDAELAVRAALERGAT
                     ELLFVGALGGQRVEHALANVLLLTLPELRDVEVMLVDGPSTVRVMSTSPGTGGCLSIT
                     GEPRDYVSLLPLSEEVTGVTTSGLAYPLSDETLIQGYTRGLSNELVGTSAEVRTMGGR
                     LAVVHTRRAALEEAK"
     gene            3967..>4187
                     /locus_tag="VMZ33_02930"
     CDS             3967..>4187
                     /locus_tag="VMZ33_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16217.1"
                     /translation="MTIRGLSLALALMAVIACTPSGQPSAPPAKVTLLTHSSFAISDS
                     LLAAFRAEHGVAVEILRGEDAGSMVNQAIL"
BASE COUNT          700 a         1385 c         1429 g          673 t
ORIGIN      
        1 tcgctcgtcg gctcgtcgct gacgatgacg acggttcggc cgtagcgctg gcgctgttcc
       61 cacggctgcc aacggctgcg cgggtccatc acgacgccgc tgaacggcgc caacaatggc
      121 tggcccagcc cggcatagat gtcgatgccg tcgtggagcc gctcgactgt gccgtcgcgg
      181 aggcggctgt ggttgtagtc ctccggcgcc ccggttcgaa cgtccagcca gttgtcccga
      241 tattggtaat tggcgtcggt acgtaacgga aagccgatat cgatgacctg gccgcgcagg
      301 tcaaactccg ccggtgaggt aggcgcgcga ccgccgacgg gcgtcggctg cggatcggtc
      361 gacggctgag gcgtgggcgt ggcacggggc ggtcgttccg tctggcgcgg cgtaggcgtc
      421 gcgccggagg gtcgtggcgt ccgaccagcg ccggcaccct gtccgccgcc gcccaccgtc
      481 ccctgcggtc gctgggacgc cccattcgcc gccgcgccac cggggcttgg gctcgcactg
      541 ccagcaccgc cacctccgcc gagcaacgtc gacagagcca ctgccccgac gagtgcgagc
      601 acgccgacga ttgcgatcgg cccgatcgca cggcgcgctg tcatcccgtc ggactcacgg
      661 gcgatcggcc gcgccatcat tcgtgtctgt tcgcggcgag tcatctgggc ctcgtcacgc
      721 ggatgtagac gagagtgacg gcggcggcgg caacgccgac cccgatcacg gcaacgaggg
      781 caagcagcgg cgcgcccacg cgattagccc ccgcacccgc gcgtgggtcg agcggatcga
      841 cgaacacggg cactggggtc ggcgtggggc cgagttggtt aagtggatcg gcagccagcg
      901 aaggcgctgc ggaaaacgcc acgatcgcca tggccagcgc gatcgccacg cgcaaggaca
      961 acaccaattt cgagcctagc agtcctgccg acgccgcgtt cagccgatca gcaagcgaat
     1021 gacgattcca gccacgacca tcaagaagcc gccgatggcg gccagagttc cgacaacgag
     1081 cctgccgcgt cgttcaccgg ccaggacgcg catcgcggcg ttctggtcga gcgaggtaac
     1141 gatgagcggt cggcccagac cagcggtaag agtcggctcg ccgtcgcgca gcaccggcac
     1201 gccgcaggct gtcgcgtgct cgacggcaga aagctgatgg atcgtcagcc gggccggcgt
     1261 gtcgggcgtc acggttgccg ccacgtccgc cttcaggtca tcgggaaggt ccgcaaccgt
     1321 cccgacggat tcacgaggga tgaccaccag gccgtccagc agtgccgcct catcgatggc
     1381 gatgctctcc gagcgcgtct ccagggcgaa cggcacggcc tcgcgctcgt ccatgagcga
     1441 cgtccagcgg ccatccccct gagcctctat tcgggtgcgt cggaatacga gcgggcgttc
     1501 gtggtcgtcg gggaactcct cgtcgctggt gatccggccc tcgacccgca cgtaggccgg
     1561 cgcgccatca gcggcgagct tccgtgcgtc ggcgatcggc acattcctcg ccgccgcaag
     1621 gagccgtccg atacgcgcgc cacggccgac cgatcgaacg agcaagaagc cgacgagcag
     1681 actccctgtg ccgaaggaaa gcagggcaac agcgagcggg ctcactcagc cgacccatgc
     1741 gcgcctgtcg gcgcacaaag aaacacccgg aggaaccttc acgaggcgtg gaccttgatg
     1801 tgatcgcagt cactagcttc gcttctggcc cctccgggct gctgtgaacg gtaacgacgg
     1861 atgtcgataa cggtcaacgg tcgttgtcca cattcggaag gttcggccgc ccagctttcc
     1921 accgttcatc cacaatcgct gtggttatgc ggcttggccg gtggttgcat ccaggtagac
     1981 gtgcaaaccg ttgtaggtcg tcagatcgag ccggccggcg acgagctgag catgggtgat
     2041 gacgtccgag tgggcagcgc gccaggcaac ctcgccgtcg ttgcgcagcg cgagtgtttc
     2101 gagctcggtc tgcatcagga catgatcgag acgggttgag gcgagcacgc tcaggacggg
     2161 tgtaccactc atgtgtgacc aggcaagctc gccgcttcgc gcatgaaatg catagacagc
     2221 gaagccgtac ttgatcagca gcaggtttgc ggtgtcgacg agggtgccct gtggctcgtc
     2281 gaagacctgc gaggccaagc gggcggtcca cgaaccactg ggactctcaa cgcggagctc
     2341 ggcccggcat gcgctagcgc cgctgccgct caactcggtg tagtcgttga gctcaccggc
     2401 cgactcggag acgccaaaga ccacatcagc ctgaccgggg ccgccccatc gaaaacgagc
     2461 tgagatgccg cccgggtact ggacggcgag ccgccccggt tccaacccag tgtcaggcgt
     2521 tcgtcggagc gctggcggtt cgctctgctt cggtcggcag gaaaccgcct gtgatcgacg
     2581 taacaacggc gtttgagagg aggcgcaggg tttgtggcgc gatcgggaag acggcattcg
     2641 gcgttccagc ggctgcccag acctgctcgt ggcggcccag gtccggatcc atgatcgtcc
     2701 gcagcacctg cttgtggccg aacggcggga tgccacctat ggcgaagccc gtcagctcgc
     2761 gcacctcact cgcgttggcg cggcgcaggc gcggctccgt cagcacggcg cctagaaggg
     2821 ccacatccac ctggttggcg ccgctggcga ggaccaggaa cgcctccagc ccatcgacgt
     2881 gctctgaaac gaagatgagg ctcttgacaa tcgcgccgac ctccgtgccc acggcacgcg
     2941 ccgcctcttc ggcagttcgc gtggactcgt cgaagaagac gacgtcgagc ttgattccct
     3001 tacgttcggc cgcgtccagc acgcgctggg tagcgtcagg tgtgaccgtc atcgcgcggc
     3061 cgagcatagc atcgggcact tttggcccgc cgaccccacc ggattgcggg tttacaatgg
     3121 gctcaattcc acacgggaca ggggagcgct ttcaagcgct gagagtgcgg gcaacgccgc
     3181 agaccctcgg aacctgatct ggttcggacc agcgaaggga gtccactcga aggcgcgccg
     3241 ttgtcccggc agctgaggta cgcgcgtctt gcgagcaatc gtcgtcgctg acggagagat
     3301 cagaggagca gcccaggcgg agccactggc tgagtggctc gccgcgcgca aggccggtga
     3361 gttgatgctc gtcgtggcgg ccgatggtgg cgcggcgaag gccgatttgc tggggcttcc
     3421 cgtcgacctg gtcgtgggcg acgccgactc cctgacagag gacgcgtttc gccgcctcgc
     3481 ggccgagggc gtcgaagtca accgctatcc ggcggacaag aacgagagcg atgccgagct
     3541 cgcggttcgc gctgcactgg agcggggtgc gacggagctg ctgttcgttg gcgcgctcgg
     3601 tggacagcgc gtcgaacacg cgttggcgaa tgtcctcttg ctcaccctgc ccgagctgcg
     3661 cgatgtggag gtcatgctcg tcgacgggcc gagcacggtc cgcgtcatgt ccacgtcccc
     3721 aggcaccggt ggctgcctct cgatcacggg ggagccgcgc gactacgtct cgctgttgcc
     3781 gctctccgag gaggtcacgg gggtgacgac gagtggcctc gcctaccccc tgagcgacga
     3841 gacgttgatc cagggttaca cccgcgggct ctcaaacgag ctcgtgggca catccgccga
     3901 ggtacgcacg atgggcggcc ggttggcagt ggttcacacg cgccgtgcgg cccttgagga
     3961 agccaaatga cgatccgtgg cctcagcttg gccttggcac tcatggcggt tatcgcctgc
     4021 acgccgagcg ggcagccctc cgcgccaccc gccaaggtga cgctgctgac ccacagctcg
     4081 ttcgcgattt ccgacagcct cctggcggca ttccgggctg agcacggcgt ggcggttgag
     4141 atccttcgcg gtgaagacgc cggctcgatg gtcaaccagg cgattct
//
LOCUS       DATLCO010000118         2783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6030695, whole genome shotgun sequence.
ACCESSION   DATLCO010000118 DATLCO010000000
VERSION     DATLCO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6030695"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2640
                     /gene="dnaE"
                     /locus_tag="VMZ33_02935"
     CDS             <1..2640
                     /gene="dnaE"
                     /locus_tag="VMZ33_02935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HUR16218.1"
                     /translation="DIDVDFEDARRGEVIEYVSRKYGSDHVAQIITFGTMLARAAIRD
                     VGRVLGLSYGEVDRIAKAIPNQLGIKLEEALEISPQLREMRDADANVKRLLELARQLE
                     GVARNASTHAAGVVISREPLTELMPLQHATNSDGLMTQYEMHGVEALGLLKFDFLGLS
                     NLTILRQAVDLVKQERGDEIDINDIPLDDTKTFELLASGETTGVFQLESPGMRRYVKE
                     LRPTSVQDLAAMVALFRPGPMDNIPAYIRRKHGQEQVTYLHPLLEPYLKKTYGIFVYQ
                     EDIMAAAIALGGFTGPEADTLGYAIRKKKSSVLRAQKDKFVQQAAERGVPPQTIDAVF
                     KAFEPFERYGFNKAHATCYGLIAYQTAYLKANYTVEYMTSVLTAFRDSTDKVAAAIAE
                     CRRLGIDVLPPDVHRSGLDFTVEGDAIRFGLLAIKNVGSGAIESIVEQRTATGSFKSL
                     MDFCNRVDLRLVNRRVLESLVRVGALNAFGHPAQLQMALDDALAAGQAAQRDRLTGQV
                     SLFDTMGADAAPLERPLPVGITEAAPRERLRWEKELLGLYLSDHPLGELAASIGEYVN
                     AYSGEMGEELDQQRVVICGVVTEIRKVITKARATMGVATLEDLQGTVEVIVFPKVYEQ
                     TESTWAEDAILLVAGRVDHKGEETVLLADAVWTWEQASAMGAAAFSAEVARGDRGRRG
                     GGNRNAYANGNGSGHGVNGYGNGVGAPTPSAVPVGPGTPAAAARLVRTIPLVSPLRGV
                     DLNGSLDVYFGTTPVRTAATALAGPSSMETDESPLPAEQMRELLVQQTAPTLPVQAAA
                     GQVLHIKLLSELQDEVLTALNGLRGVIHEHPGDTAVVLHVPSGAGRFQRMELRLRVAY
                     DAELVASIGRRVGEKLFELSLGS"
BASE COUNT          482 a          891 c          936 g          474 t
ORIGIN      
        1 gacatcgacg tcgacttcga ggatgcccgc cgtggcgagg tcatcgagta cgtgtcgcgc
       61 aagtacggct ccgaccacgt ggcccagatc atcaccttcg gcacgatgct cgctcgtgcg
      121 gcgatccgcg acgtcggccg cgttctcggc ctctcatacg gcgaagttga ccggatcgcg
      181 aaggcgatcc cgaaccagct cggcatcaag ctcgaggagg cgctcgagat ctcgccgcag
      241 ctgcgcgaga tgcgcgacgc cgatgcgaac gtaaagaggc tcctcgagct cgcgcggcaa
      301 ctggagggcg tggcacgcaa cgcgtcgacg cacgctgctg gggtcgtgat cagccgtgaa
      361 ccgctgacgg agctcatgcc gctccagcac gccacgaaca gcgacgggct gatgacgcag
      421 tacgagatgc atggcgtcga ggctctcggc ctgctcaagt tcgacttcct gggcctgtcg
      481 aacctcacga tcctccgcca ggcggtggac ctcgtgaagc aggagcgcgg cgacgagatt
      541 gacatcaacg acatcccgct tgacgacacc aagacgttcg agctgttggc ctcaggcgag
      601 acgaccggcg tcttccagct cgagtcgccg gggatgcgcc gctatgtcaa ggagctccgt
      661 ccgacctcgg tgcaggacct cgcggccatg gtggcactgt tccgaccggg cccgatggac
      721 aacatcccgg cctacatccg tcggaagcac ggccaggagc aggtgacata cctccatccc
      781 ttgcttgagc cgtacctcaa gaagacgtac gggatcttcg tctaccagga agacatcatg
      841 gccgcggcga tcgcgctcgg tggtttcacc gggcctgagg cggacacgct cggctacgcc
      901 attcgcaaaa agaagtcgtc cgttctgcgg gcgcagaagg acaagttcgt ccagcaggcc
      961 gcggagcgcg gagtgccgcc gcagaccatc gacgcggtgt tcaaggcatt cgagccgttt
     1021 gagcgctacg ggttcaacaa ggcccacgcg acgtgctatg ggctgatcgc ctaccagacc
     1081 gcgtatctca aggcgaatta cacggtcgag tacatgacct ccgtcctgac ggcgttccgt
     1141 gactcgaccg acaaggtggc ggcagccatc gccgaatgcc gccggctggg cattgacgtg
     1201 ctgccgccgg acgtccatcg cagcggtctt gacttcaccg tcgagggcga tgcgatccgt
     1261 ttcggtctcc tcgcgatcaa gaacgtaggc agtggcgcga tcgagtcgat cgtcgagcag
     1321 cgcactgcca cgggttcgtt caagtccctg atggacttct gcaatcgggt cgacctgcgc
     1381 ctggtcaatc gtcgcgttct ggaatcattg gttcgtgtcg gcgccctcaa cgccttcggc
     1441 catccggcac aactgcagat ggcgctcgac gacgcgctgg ccgctggcca ggccgctcag
     1501 cgcgaccggc tgaccgggca ggtatcgctc ttcgacacga tgggtgccga cgcggcgcct
     1561 ctcgagcggc cactccccgt tgggattacg gaggccgcac cgcgcgaacg tctgcgctgg
     1621 gagaaagagt tgctcggcct gtacctgtct gatcacccgc tgggcgagct ggcggcttcc
     1681 atcggcgagt acgtcaatgc gtacagcggc gagatggggg aggagctcga tcagcagcgg
     1741 gtcgtgatct gcggcgtcgt taccgagatc cgcaaggtga tcaccaaggc gcgggcgacg
     1801 atgggcgtcg ccacgctcga ggacctacag ggaacggtcg aagtgatcgt cttcccgaag
     1861 gtgtacgagc agaccgaatc aacgtgggcg gaggacgcga tcctgctcgt cgcggggcgc
     1921 gtcgaccaca agggcgagga gactgtgctg ctcgccgacg ccgtctggac gtgggaacag
     1981 gcctcggcga tgggcgcggc tgcgttttcg gcggaggtgg cgcgtggcga ccgtggtcgg
     2041 cgaggcggcg gcaacagaaa cgcctacgcc aatgggaatg gaagcggcca cggggtcaac
     2101 ggctacggaa acggggtagg ggcgccgacg ccgtcggccg tgccggtagg tccgggaact
     2161 ccggccgcgg cggcgcgcct cgtccgaacg atcccgctcg tgtccccgtt gcgcggcgta
     2221 gatctaaacg ggtcactcga cgtgtatttc ggcacgacac ccgtgcgcac cgcggctacc
     2281 gcgttggccg gcccctcatc aatggagacc gacgaatcgc cgttgcccgc cgagcagatg
     2341 cgtgagctgc tggtccaaca gaccgcgccg acgctgccgg tgcaggcagc tgcgggtcag
     2401 gtgcttcaca tcaagctctt gtccgaattg caggatgagg ttttgacagc gctcaatggg
     2461 ttgcgtggcg tcatccacga gcatcccggt gacacggcag tcgttctgca cgtcccgtcc
     2521 ggcgcgggtc gtttccagcg gatggaactt cgcctgcgcg tcgcctacga cgccgagctg
     2581 gttgcgtcga tcggccgtcg cgtgggcgag aagctgttcg agctgagcct cgggagttag
     2641 cccgctcctc cgctagccgg tgacagcccg ctccaggccg cctgtctcgg gcaacgtcac
     2701 gcggatctcg tcctcacaga cgttgcatct gaagatgcgc ccgctcgccc acgagttctc
     2761 ggggtcggtg tcagccgggt cgt
//
LOCUS       DATLCO010000119         4246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4605099, whole genome shotgun sequence.
ACCESSION   DATLCO010000119 DATLCO010000000
VERSION     DATLCO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4605099"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1020)
                     /locus_tag="VMZ33_02940"
     CDS             complement(244..1020)
                     /locus_tag="VMZ33_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16219.1"
                     /translation="MIIRPITSILVALLLGATTGGCVTVAPAGSSGPSVALPSIAVPS
                     PLLPSAPGLSISIPSVVVPTIAPATPERTRKPRPTETATPTATATATATATATATATA
                     TATATATATATATPTAAPTLNFSLTPNYGSIALTSGFVPDPHTIAVTSGGGVNVAYLG
                     GGCTGYATSAPDYSVNFTSGAFPTLRFYFVGTSGDSTMIVNGPGASYSCSDDSFGTLN
                     PTVDFNSPSSGRYDIWIGSFASGTSISGTLYVTENTGNHP"
     gene            1315..3240
                     /gene="dnaG"
                     /locus_tag="VMZ33_02945"
     CDS             1315..3240
                     /gene="dnaG"
                     /locus_tag="VMZ33_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HUR16220.1"
                     /translation="MKSKINIADLVGESVQLRKAGASFKGLCPFHNEKTPSFTVTPSR
                     ETWKCFGCGRGGDLFNFVMERDAIDFPEALRRLAARAGVEISERTSRDDARRKRLRDA
                     LDAAIAFYHRTLTESRHGQPALDYMRTRGFTEETLSNHQIGFAVDAWETLTRALVEKR
                     DFREEELEASGLVARRSGRRGVYDRFRGRIIFPIRDASGNATGLGGRIVGSGQRATSE
                     RDAGPKYLNSPQTLLFDKSRTLYLVDRAKSPMRKQGVAVLVEGNTDALMAHQQGFDNV
                     VGTLGTALTPAQVELVTRYAPRIALAYDVDAAGQGAATFGATELSALVGEIERSAHKG
                     RLTDVSIVRLPAGKDPDEVMRDEPETWRTATESTLPIIEYLIDTFARRHDPRTISGRE
                     KLVAAVMPTIRRVTNPTQRDGYLQLLSRRSGVDERVLLEELRRPDASARVMGRAPEAH
                     VGSKINLEAVLSSPDALDPEAVARTLEPIESALLHLLLVRPYVRNALSGRLTPDLFVT
                     TPARELWRQVEASPVVGFERAAFVDSLEPTLAAVARTLFARIDPLPEDEDALGQAMEQ
                     SLLGLERNRMTERLEFMRAELSEAEAAGDAEVAERLRRDVQELQAERLDLDRRRENTT
                     VLTSHRNRPTAATAAST"
     gene            3255..>4246
                     /locus_tag="VMZ33_02950"
     CDS             3255..>4246
                     /locus_tag="VMZ33_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HUR16221.1"
                     /translation="MDSSDKSLVASVLERGKRRRDEDGMLDGNRNDELDEPNAGLSAA
                     AELGVDVAIDETDKQPWEEPPAKLDAETAEEPSAEELEAIAADMIGIDDPVRMYLKEI
                     GKVPLLNAEEEVVLAKAIELGEQMVDEPQKAILSLWEWTRNNTESKSREKHPQHRLPY
                     KDESDRIVRGAFTAAEKDGLLQATTDFHFVKAGKSVEGDVAKARLKEASHVVKAYNEA
                     PTADTFTELVDFAFLSAHSPDAETHDNAAIRQTYDWTLGVAHEALRRWIFGGHDAELI
                     AEMGWSPEQDLSLKPRDRRGVLVRIGREGREQLTSANLRLVVSIAKKYIGRGMS"
BASE COUNT          780 a         1340 c         1441 g          685 t
ORIGIN      
        1 cgacgttgac gcacgcgagc caccgtgacg cggagggcat ccgacgcggg cgatatgcac
       61 ggaacccgcg acggctgggc tgccgagact gcgagcgttg cccgaatggg ctctcacatt
      121 ctaatcgatt tggccccaac cagccaccga cgaccctgga taggcggtca tctccgagaa
      181 cttcgcaccg tcgcgtaaga gctcagccag ggcgtgtcga tccagatcgt tcatgcccgt
      241 gcctcacggg tggttgccgg tgttctcggt gacgtaaaga gttccgctga tggacgtccc
      301 cgaggcgaat gaaccgatcc agatgtcgta gcgaccgctc gacggcgagt tgaagtcgac
      361 cgtggggttg agggtcccga aggagtcgtc gctgcacgag tacgaggcgc ccgggccgtt
      421 gacgatcatg gtcgaatcgc ctgacgtgcc cacgaaatag aaccgaagcg taggaaacgc
      481 gcccgacgtg aagttcacgc tgtaatcggg ggcggaggtc gcgtaacccg tgcacccgcc
      541 tccgaggtag gcgacattca cgcctccgcc gctcgttacc gcgatggtgt gaggatctgg
      601 cacgaagccg ctcgtcagtg ctatcgagcc atagttaggc gtcaagctga agttgagggt
      661 tggcgctgcg gtgggcgtgg cggttgcggt ggccgtggcc gtcgcggttg cggttgcggt
      721 tgcggtggcc gtcgcggttg cggttgcggt ggcggtggct gtggcagtcg gcgttgcggt
      781 ctcggtcggg cgcggcttgc gggtgcgttc cggcgtggct ggagcaattg tcggcacgac
      841 cacggacgga atcgaaatcg acagaccagg ggcagacggc aggagtggcg acggcacggc
      901 aatcgacggc agggcaacgg agggcccgct tgacccggcc ggtgcgaccg tgacgcagcc
      961 accagtggtg gcgcccaaca ggagtgccac aaggattgaa gtgatcggcc tgatgatcat
     1021 cggtaacggc tcccctcaaa gcgtcacggc aggtgtgcgc ggcaagttct gcgaagcgca
     1081 gcgtacacct cgcagtcgcc cagagaaggt caaccgcact ccctgtcaat actccctatg
     1141 ggtattgaca agcgtcgcct ttcgacactt gtcaagcccc ttgtcaaggg ggtagaattg
     1201 cgccactgag ccttgtcaag agttgacaag gccatacgtg tgtccggctc atagccgtgg
     1261 gcgtgcacac gggagccagg aggcgccacc acgtcagtcg gtaccgccgc tgagatcaaa
     1321 tcgaagatca acatcgccga cctcgtcggt gagagcgtcc agttgcgcaa ggccggcgcg
     1381 tcgttcaagg gcctgtgccc attccataac gagaagacgc catccttcac cgtcacgcca
     1441 tcgcgcgaga cgtggaaatg cttcggctgc ggccgtggcg gcgacctctt caacttcgtc
     1501 atggagcgcg acgcgatcga cttccctgag gcgctgcgcc gcctcgcggc gcgcgccggc
     1561 gttgagatca gcgagcgcac aagccgtgac gatgcgcggc gtaagcgact ccgtgacgct
     1621 ctggacgcgg ccatcgcctt ctaccaccgc actctcaccg agagccggca cggccagccc
     1681 gcgctcgact acatgcgtac ccgtgggttc acggaggaga cgctcagcaa ccaccagatc
     1741 ggcttcgcgg ttgatgcctg ggagacgctg acgcgcgccc tcgtcgagaa gcgcgacttc
     1801 cgcgaggagg agttggaagc gtctggcctg gttgcgcgac gcagcggccg gcgcggtgtc
     1861 tacgaccgtt tccgcggccg catcatcttc ccgatacgtg acgcgtcggg caacgccacg
     1921 ggcctcggcg gtcggattgt cggaagcggg cagcgggcca ccagcgagcg cgacgctggg
     1981 cccaagtacc tcaattcgcc gcagaccctg ctattcgaca agagccggac gctgtacctg
     2041 gtggatcgcg ccaagtcacc catgcgcaag cagggcgtgg ctgtgctcgt cgagggcaac
     2101 accgatgcgc tcatggccca tcagcagggc ttcgacaacg tggttggcac gctgggcacg
     2161 gcgctgacac cggcgcaggt cgagctcgtg acgcgctacg cgccgcgcat cgccctcgcg
     2221 tacgacgtcg acgccgcggg gcagggtgcg gccacgttcg gtgccacaga gctatccgct
     2281 ctcgtcggtg agatcgagcg gagtgcgcat aagggccggc tcactgacgt gtcgatcgtc
     2341 cggctgccag ctggcaagga ccccgacgaa gtcatgcgcg acgaacccga aacctggcga
     2401 acggcaaccg aatcgaccct gccgatcatc gaatacctaa tcgacacgtt cgctaggcga
     2461 cacgacccac gcacgatctc cggccgcgag aagctcgttg cggcggtgat gccaaccatt
     2521 cgccgcgtga ccaacccaac gcagcgcgat ggctacctcc agctcctctc gcggcgaagc
     2581 ggcgtcgacg aacgtgtcct gcttgaagag cttcgtcggc cagatgcgtc ggcgcgcgtg
     2641 atgggtcggg ccccggaagc ccatgtcggc tcgaagatca acttggaggc cgtcctgtcg
     2701 agcccggatg cactcgatcc cgaggctgtc gcacgaacgc tggagccgat cgagtcggcc
     2761 ctccttcacc tgctgctcgt ccggccatac gtgcgcaacg cgttgtcggg tcggctaacg
     2821 cctgacctct tcgtcaccac gccggcgcgg gagctgtggc ggcaggttga ggcctcgccg
     2881 gtggtgggct tcgagcgcgc ggctttcgtc gactccctgg agccgacgct ggctgcggtc
     2941 gctcgcactc tgttcgcacg catcgacccg ctgcctgagg acgaggacgc gctcggtcag
     3001 gccatggagc agagcttgct cggcctcgaa cggaatcgca tgactgaaag gctcgagttc
     3061 atgagggccg agctgtccga ggctgaggcc gcgggcgacg ctgaggtcgc ggagcgcctg
     3121 cgccgcgatg tccaggagtt acaggcagag cggctcgatc tcgatcggcg ccgcgaaaac
     3181 acaaccgttt tgacctcaca ccgaaaccga cccaccgcgg ctacggccgc cagcacctag
     3241 gaggcgcgaa gaccatggat tcaagcgata agtcactagt agcgagcgtt ctcgagcggg
     3301 gcaagcgtcg ccgcgacgag gacgggatgc tcgacggcaa ccggaacgac gagctcgacg
     3361 agccgaacgc gggcctctcg gcggcggccg agctcggcgt tgatgtcgcg attgacgaga
     3421 cggataagca gccctgggaa gagccgccgg caaagctcga tgccgagacg gccgaagagc
     3481 cgtccgccga ggagctcgag gccatcgccg ccgacatgat tggcatcgac gacccggtgc
     3541 ggatgtacct caaggagatc ggcaaggtgc cgcttctcaa cgctgaggaa gaggttgtcc
     3601 tggccaaggc cattgagctc ggcgagcaga tggtcgatga gccgcaaaag gcgatcctct
     3661 cgctttggga gtggactcgc aacaacaccg aatcgaagtc gcgcgagaag cacccccagc
     3721 accggctgcc gtacaaggac gagagcgatc gcatcgttcg tggcgcgttc acggccgcgg
     3781 aaaaggacgg gttgctgcag gcgaccaccg acttccactt cgtgaaagcc ggcaagtcgg
     3841 ttgagggtga cgtggccaag gcccggctca aggaggcgtc gcacgtcgtc aaggcgtaca
     3901 acgaggcgcc cacggcagac actttcacgg agctcgtcga cttcgcgttc ctgtcggcgc
     3961 actctcccga cgcggagacg cacgacaacg ccgccatccg ccagacgtac gactggacgc
     4021 tgggagtggc ccatgaggct cttcgacgct ggatcttcgg cggtcacgac gccgagctca
     4081 tcgccgagat gggctggagt cctgagcagg acctgtcgct gaagccgcgc gatcgacgtg
     4141 gcgttctggt gcggatcggc cgcgaggggc gcgagcagct gacgtccgcc aacctgcggt
     4201 tggtggtgtc gattgccaag aagtacatcg gccgtggcat gtcctt
//
LOCUS       DATLCO010000120         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3865011, whole genome shotgun sequence.
ACCESSION   DATLCO010000120 DATLCO010000000
VERSION     DATLCO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3865011"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VMZ33_02955"
     CDS             <1..459
                     /locus_tag="VMZ33_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HUR16222.1"
                     /translation="DGVGEGNGLLNAEPVLIYAKDLIQLRATPISEWPQFRFWTLDDL
                     GSAADLEVPPDLESYQVWHSRGLPKGPICTPGLSSLQAALNPNTEDGFIYFLAKGDGS
                     NGHVFAHTYEEHLRNIEQYMGGGASSTPTPETASPEAVPTDAFVSPGLTP"
     gene            456..1637
                     /locus_tag="VMZ33_02960"
     CDS             456..1637
                     /locus_tag="VMZ33_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HUR16223.1"
                     /translation="MKPTGEDLRRWADADGAARPRRLDGLRERMTSAGVDAYFGVRRE
                     NTRYLTGFELGEGEEKVAGNSGQFIVGRDDVMVLADSRYTAAATKQCSGSHIERVYND
                     IYDRWPEMLASLGDVKRVAVEAGFVSHAMWQRLDEAAPSVELVPAEGWVESLRQVKEP
                     SELERVAAACAVADAALQQLLPQIKAGVTEHELALQLEWEMRTNGAEALAFDVACLAG
                     AQAALPHGAPTDRSVKNGEVLLFDFGAQVAGYRSDMTRTLFVGEPTAQSLEIYEVVAR
                     AQEASFEALSQALADGSRPSGRSIHMIAKRVIVDAGYGDHFGHGLGHGIGLATHEMPG
                     LGMSAPDVPLPSPTVFSIEPGIYLDGVTGVRIEDLVVFDAEQKRLERLTMFPREVTTV
                     G"
     gene            1690..2247
                     /gene="efp"
                     /locus_tag="VMZ33_02965"
     CDS             1690..2247
                     /gene="efp"
                     /locus_tag="VMZ33_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HUR16224.1"
                     /translation="MISTGELRKGVVIELDNELWQILDYHHIKMGRGSAQVRIKLRNI
                     RRGQTVERSFQAGEKWPRAQLETRPVQYLYSDGTEYHFMDTQSYEQFSMTAEKLGDAA
                     QYLIDGMTLERTSYETETIGVELPVTVEQKVVETDPGFAGDTTGGARKPATTETGLVV
                     QVPLFVSVGDTIRIDTRTGDYQTRV"
     gene            2340..>2932
                     /gene="xseA"
                     /locus_tag="VMZ33_02970"
     CDS             2340..>2932
                     /gene="xseA"
                     /locus_tag="VMZ33_02970"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HUR16225.1"
                     /translation="MTDPLRPMEPIAPPIQNAASSLPILSVAELTAQIKGALRAQPRL
                     REVWVEGEVGQVSISAAGHCYFTLKDERAQLKCVIFREDRIMIPFEARTGLRLVAHGN
                     IDVFDAQGVYQLYVRTLQPSGFGDLALRFEALKAKLQAEGLFDAAKKRKLPTYPQAIG
                     VVTSLNGAVLHDIRRVLARRWPMARVVVSACQVQGTGA"
BASE COUNT          525 a          964 c          977 g          466 t
ORIGIN      
        1 gacggcgtgg gcgaaggcaa tggtttgctc aatgccgagc ccgtcctgat ctatgccaag
       61 gacctcattc agctgcgcgc tacgcccatc agcgagtggc cgcagttccg gttctggacg
      121 ctcgatgacc tcggttcggc cgcggacctg gaggtgccac ccgacctgga gagctatcag
      181 gtgtggcatt cgcgcgggct acccaagggg ccgatctgca cgcccggtct gtcatcgctc
      241 caagctgcgc tcaacccgaa caccgaggac ggcttcatct atttcctggc aaagggtgac
      301 ggctccaacg ggcacgtctt cgcccacacg tacgaagaac acttgcggaa catcgagcag
      361 tacatgggtg gcggtgccag ttccacccct acgcctgaaa ccgcctcgcc cgaagccgtg
      421 ccgaccgacg ccttcgtctc acccggttta acgccatgaa accgacaggg gaggacctcc
      481 ggcgctgggc ggacgccgat ggggcagcac ggccacgcag gctggacggg ctgcgtgagc
      541 ggatgaccag tgcgggtgtt gacgcttact tcggtgtccg ccgcgagaac acgcgctacc
      601 tgactgggtt cgagctcggg gagggggagg agaaggtcgc cggcaactcg ggccagttca
      661 tcgtcggccg tgacgatgtc atggttctcg ccgactcgcg ctacaccgcc gccgccacca
      721 aacagtgcag cggctcgcac atcgagcgcg tctacaacga catctacgac aggtggcctg
      781 agatgctggc aagcctcggc gacgtcaagc gggtggcggt tgaggcggga ttcgtcagcc
      841 acgccatgtg gcagcgcctc gacgaggccg cgccgagcgt ggagcttgtg ccggctgagg
      901 gttgggtcga gtccctgcgc caggtaaagg agccctcgga gctggaacgc gtcgcggccg
      961 cgtgtgcggt tgccgacgcc gccctccagc agctgctgcc gcagatcaag gccggcgtta
     1021 ccgagcacga actcgccctg cagctcgaat gggagatgcg caccaatggc gccgaggcgc
     1081 tcgcctttga tgtggcatgc cttgccggcg cgcaggcggc gctgccccac ggcgcgccca
     1141 cggatcgaag cgtcaagaac ggcgaggtcc tgctgttcga cttcggggct caggtggcgg
     1201 gctatcgatc ggacatgacg cggaccctct tcgtgggtga gcccaccgcg cagagcctcg
     1261 agatctacga ggtcgtggcg cgcgcacagg aggccagctt cgaggccctg tcgcaagccc
     1321 tggctgacgg ttcacgaccg agtggcaggt ccatccacat gatcgccaag cgcgtgatcg
     1381 tggacgccgg gtacggcgat cacttcggcc acggcctcgg ccacggcatc gggttggcga
     1441 cacacgagat gcccggcctg gggatgagcg cacccgacgt gccactgccg tcaccaacgg
     1501 tgttctcgat cgagccgggg atctacctcg acggcgtcac cggggtgcgc atcgaggacc
     1561 tcgtggtctt cgacgcggag cagaagcgac tcgagcgcct gacgatgttc ccgcgtgaag
     1621 tcacgacagt aggctgacga ctcggtcggc cagtgggcca cccctgcgcg cgtcggctag
     1681 gatctgagcg tgatttcgac tggagaactt cgtaaaggcg tcgtcataga gctcgacaat
     1741 gagctgtggc agatcctcga ctaccaccac atcaagatgg gccgcggctc agcccaggtg
     1801 cgcatcaagc tgcgcaacat ccgtcgcggc cagaccgttg agcgcagctt ccaagccggc
     1861 gagaagtggc cgcgcgcgca gctcgagact cgacccgtgc agtacctgta cagcgacggc
     1921 accgagtacc acttcatgga cacgcagagc tacgagcagt tctcgatgac cgccgagaaa
     1981 ttgggcgatg ccgcccaata cctgatcgac gggatgaccc tcgagcgtac gtcgtacgag
     2041 acagagacga ttggcgtcga gctgcccgtc accgtggagc agaaggtggt cgaaaccgat
     2101 ccgggcttcg ccggcgacac gaccggtggc gcgcgcaagc cggccacgac cgaaaccggg
     2161 ctcgtggtcc aggtgccgct gttcgtgagt gtcggcgaca cgatccgcat cgacacccgc
     2221 accggggact accagacccg cgtctaggcc atagacatga ctgagccacg cgtcagcaga
     2281 gcgttcattc ttgcccgtcg acacgacgcg ctgggtcttt gaggccggta ggtagagcgg
     2341 tgacggaccc cctccggccg atggagccga tcgcgccacc gatccagaac gcggcgtcgt
     2401 cgctacccat cctgtctgtc gcggagctca cggcgcagat caagggcgcg ctgcgtgccc
     2461 agccgagatt gcgcgaagtg tgggtcgaag gcgaagtcgg ccaggtcagc atcagcgccg
     2521 ccggccattg ctacttcacg ctcaaggatg aacgcgcgca gctgaagtgc gtgatcttcc
     2581 gcgaggaccg gatcatgatc ccgttcgagg cgcgcacggg gctgcgcctc gtggcccacg
     2641 gcaacatcga cgtcttcgat gcccagggcg tctaccagct ctacgtccgc acgctccagc
     2701 cgagcggctt cggcgacctg gcgttgcgct tcgaggcgct caaggcaaag ctgcaggctg
     2761 agggcctttt tgacgcagcc aagaagcgga agctgccgac gtacccgcag gcgatcggcg
     2821 ttgtcaccag cctgaacggc gccgtgctcc atgacatccg ccgcgtactc gcccgccgct
     2881 ggccgatggc ccgcgtcgtc gtctcggcct gccaggtgca gggcaccggc gc
//
LOCUS       DATLCO010000121         5698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6032235, whole genome shotgun sequence.
ACCESSION   DATLCO010000121 DATLCO010000000
VERSION     DATLCO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6032235"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VMZ33_02975"
     CDS             complement(<1..488)
                     /locus_tag="VMZ33_02975"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR16226.1"
                     /translation="MAQATVEPAPKEELSVEAFADRARAIETEVKSVIVGQDNLVRQT
                     LICMIANGHALLEGVPGLGKTMLVKTFSDVLDCSFNRIQFTPDLMPADIVGTNVLVEQ
                     DGQRQFRFQPGPVFANLLLADEINRATPKTQSALLEAMQEQQVSIARERHKLEPPFFV
                     LAT"
     gene            complement(488..2311)
                     /locus_tag="VMZ33_02980"
     CDS             complement(488..2311)
                     /locus_tag="VMZ33_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16227.1"
                     /translation="MIDPQLLEMRRSLGRFRRRVWLRRVVRDGSVILAATLAAELALA
                     VVVRLTPFEWHLLAAVAIAAIGAVALIADAIRVRPSIAEAALAVDTEDGLADRVSTAL
                     ALAKARPELARADTSGESHDAEGATYAHLVRLQRRDALTALVGADPRGLRIPLPRRQG
                     ATSMLCALLLIPAMLLPNAQNDVLQQRENMRETAEQQAQLLEESATKLAEGRTAPDPR
                     VDLAKEIRELAEQLRNNPEDLNTNLARLGSLEDAIRSRLDPNNEQRAAAISSLSRSLS
                     KASTGSDSNPQGDPQKTNEDLDDLKQRLGEMTEEEREALARELSQMDSTARQAGANAQ
                     TALSDAVRAIQSGDVEAAGEALDRLGDEIERGQEQVETNRDLARTAGDLQQARRDLAN
                     AGQQGEQGSQGQQGNQGQQGNQGQQGQGQGQGQGQGQQPGQRPGQGQGQGQGQGQGQG
                     QGQGQGQGQGQGQGQGQGQGQGQGRGQGQGQGQGSLSGGGSNARYLGSGSGGNSGFGG
                     PNQGNRQFGTGDLDPVFADFDRLGNPGDPSYIAGQGGDGQIQQGQGQGQGTNNQSVVP
                     YTDVYQDFYDFAITSLDRSYVPIDVRDYVRDYFSSLNPGAE"
     gene            complement(2304..3674)
                     /locus_tag="VMZ33_02985"
     CDS             complement(2304..3674)
                     /locus_tag="VMZ33_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16228.1"
                     /translation="MTTAVAGVRRGGRFIGSRLGRFWSGISAVSIKELRGRMRGRRAF
                     VVLTVYLLLLALFAFAIYVYLKQQATLTTSTIGVNPGIPRDFPGFPLPNQQTLSNGTA
                     LSAAIGHGIFGGLLFLETLLVLVLAPAFTTGAISLEREKQTLDMLVTTPLSSLGMVIG
                     KLFSALTYVFLLIIASIPLASLVFVFGGVGPEDLIRAYVFLFALAFGMGAVGLFISAI
                     VKRTQTATVVTFVLVLVLSIGTAAAHQFWQMTSRPSTTSVILAQRPGKAPEAVLWFNP
                     FVADLDMVCATAPDTYHASCEYISSVVNKPYFGTPTQTNPDFPIFEGDPVPMPAQPGV
                     VNDKPGVDAPAMEAIAAPCPDGARCVDPGINAQAQQQVSFGFPRDTFWPKSAAAFTIL
                     GIALTLASAQLVSPTRRLRLRRPGWLRIPGRRNQIAVSTSVSHEPEVTVDEPVTVTEP
                     PEKTND"
     gene            complement(3671..4660)
                     /locus_tag="VMZ33_02990"
     CDS             complement(3671..4660)
                     /locus_tag="VMZ33_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16229.1"
                     /translation="MTIALRTERLTKRYGRSLAVAGVELEVEQGEIFGLVGPNGAGKT
                     TTLKMLATLLPPSAGDAEIVGHSIRRDPDAVRNVIGYMPDTFGVYDDMRVWEYLDFFG
                     RCYGLSSQRRRQMIGDLLELVDLADKRSAYVQELSRGMQQRLCLAHALVHDPSVLLLD
                     EPASGLDPRARVELRELLRELRSLGKTIVISSHILPELEELCTSVAIIDHGRVLASGR
                     VDDIAERFRVGGVYRATVVGDEAAFAAAQRHFAADPQVASVVTRPTGELEFALRGDAD
                     AASGVLTSAIRSGVRLASFGPAASDLEELFLQITDAHAASSNAGQPRSTEVQA"
     gene            complement(4657..>5698)
                     /locus_tag="VMZ33_02995"
     CDS             complement(4657..>5698)
                     /locus_tag="VMZ33_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16230.1"
                     /translation="LERGKLTGTVTNRSLMKLENVAIVFSGGAAILSDLEPAQSKEVE
                     LDVTTSPFFGYALSEAIFGTTFPRDSATARTISTRRSVLDQLFPGGSQGAADAPLLLA
                     WSKGPVLAVELPGDEPNRVGDGLYMVPLAMAISSDQIFADQMLRRDIVDSSAATAWAD
                     ASSMYLSRGTMTVEVHPARFDGAFAATALEIALTQGETRVLRGTGEFVEPLPAEQQPP
                     QDDPLSDLEPSPTPETTCPPGVECPAPAPTAAPADGKGEAEPGRPPVDPGAVGLDPMP
                     DYQLFDRTSGLWVQFPHPSMTQSYRISNPERYVDESGRVQFRFVNSNDPGQFGEDQKY
                     FNLLVRLEGTIE"
BASE COUNT          924 a         1815 c         1985 g          974 t
ORIGIN      
        1 gtcgcgagga cgaagaacgg cggctcgagc ttgtgccgtt cccgcgcgat ggaaacctgc
       61 tgctcctgca tcgcctcgag cagtgccgac tgagtcttcg gtgtggcgcg gttgatctcg
      121 tccgcgagaa gcaggttggc gaatacgggt cccggctgga accggaactg gcgctggcca
      181 tcctgctcga ccaggacgtt cgtgccgacg atgtccgccg gcatcaggtc gggcgtgaac
      241 tggatgcgat tgaacgagca atcgaggacg tcagagaagg tcttgacgag catcgtcttg
      301 ccgaggccgg gcacgccctc gaggagcgcg tgcccgttcg cgatcatgca gatcagcgtc
      361 tgacggacga gattgtcctg gccgacgatg accgacttga cctcagtctc gattgctcgc
      421 gcgcggtcgg cgaaggcctc gactgagagc tcctcctttg gcgcgggctc gaccgttgct
      481 tgggccatta ctcggctccc ggattgagcg aggagaagta gtcgcgcacg taatcgcgca
      541 catcgatcgg cacgtagctg cggtcgaggc tcgtgatcgc gaagtcgtag aagtcctggt
      601 agacgtcggt gtacgggacg acggactggt tgttggtgcc ctgaccctgg ccctgaccct
      661 gctgtatctg gccatcaccg ccctggcccg cgatatacga agggtcgccc gggttgccca
      721 gacgatcgaa gtcggcgaat acggggtcga ggtcgccggt gccgaactgg cggttgcctt
      781 gattcggacc accgaagccg ctgttgccgc cgctgccgct accgaggtat cgggcgttcg
      841 agccgccgcc gctcaggctc ccctggccct gaccctggcc ctggccccgg ccctggccct
      901 ggccctgacc ctggccctga ccctggccct ggccctgacc ctggccctgg ccctggccct
      961 ggccctgacc ctggccctgg ccctgaccct ggccctggcc ctggccggga cgctgaccgg
     1021 gctgctgccc ctgtccttga ccctgaccct gaccttggcc ctgttggccc tgattacctt
     1081 gctgcccttg gttgccttgc tggccctggc tcccttgctc accctgttgg ccggcattgg
     1141 ccagatcacg gcgggcttgc tgcaggtcgc ccgcggtccg cgcaaggtcg cggttcgtct
     1201 ccacctgctc ctggccgcgc tcgatctcgt caccgagtcg gtcaagcgct tcgcctgccg
     1261 cctcgacgtc gccagattgg atggctcgaa cggcatcgga gagagccgtt tgcgcgttcg
     1321 cgcccgcctg tcgagcggtg gagtccatct gtgagagctc ccgggcaagg gcctcgcgct
     1381 cttcctcggt catctcgccg aggcgctgct tgaggtcgtc gaggtcctca tttgtctttt
     1441 gagggtcacc ctgagggttg ctgtcggagc cagtggaggc cttgctcaac gagcgtgaga
     1501 gcgacgagat cgctgcggca cgctgctcgt tattcggatc gagtcgcgac cggatcgcgt
     1561 cctcgagcga gccgaggcgc gccaggttgg tgttcaggtc ctccggattg tttcggagct
     1621 gctcggcaag ctcgcggatc tctttggcga ggtcgacacg cggatcggga gccgtacgcc
     1681 cctcggccag cttcgtagcc gactcctcga ggagctgagc ctgctgctcg gccgtctcgc
     1741 gcatgttctc gcgctgctgc aggacgtcgt tctgtgcgtt cggcaggagc attgccggga
     1801 tcaggagaag ggcacacagc atgcttgtgg cgccctgccg gcgcggcagc gggatacgca
     1861 gcccgcgtgg atcggcgccg acgagcgcgg tcagagcgtc gcgacgctga agacggacga
     1921 ggtgcgcgta ggtagctccc tcggcgtcgt gcgactcgcc ggaagtgtcg gcgcgcgcga
     1981 gttcgggccg ggccttggcg agtgcgaggg cggtggaaac ccggtcggcg aggccatcct
     2041 cagtgtcgac cgcaagtgcg gcctcggcaa tggacgggcg gacgcggatc gcgtcggcga
     2101 tgagagccac ggcgccgatc gctgcgatgg cgacggcggc gaggaggtgc cactcaaagg
     2161 gggtcagccg caccacaacc gcgagagcca gctcggcagc gagcgtcgcg gcgaggatga
     2221 cgctgccgtc gcgaacaacg cggcggagcc agacacggcg tcggaaacgg ccgagtgagc
     2281 ggcgcatttc cagcagctgc ggatcaatca ttcgtcttct ccggtggttc ggtgacggtc
     2341 accggctcgt ccacggtgac ttccggctcg tggctaacag acgtggaaac tgcgatctgg
     2401 ttccgccggc ctggaattcg gagccagcct ggccgccgaa gccgcagacg acgggtcggg
     2461 gatacgagct gcgcggacgc gagtgtcagc gcaatgccga gaatggtgaa cgcggcagcg
     2521 ctcttgggcc agaaggtgtc ccgcgggaaa ccgaacgaga cctgctgctg tgcctgggcg
     2581 ttgatgccgg ggtcgacgca tcgtgccccg tcaggacatg gcgcggcgat ggcttccatc
     2641 gcgggcgcgt cgactccggg cttgtcgttc acgaccccgg gctgtgcggg catcggcacc
     2701 ggatcaccct cgaaaatcgg aaagtcaggg ttcgtctgtg tcggcgtgcc gaagtagggc
     2761 ttgttcacga cgctcgagat gtactcgcac gaggcgtgat acgtgtctgg cgcagtggcg
     2821 cagaccatgt ccaggtcggc aacgaacggg ttgaaccaaa gcaccgcctc aggcgctttg
     2881 cccggccgct gggccaggat cacgctggtc gtggacggtc gcgatgtcat ctgccagaac
     2941 tggtgcgccg cggcggtgcc tatcgacagc acaagcacga gcacgaacgt caccactgtc
     3001 gcggtttggg tgcgtttgac gatggccgat atgaagagcc cgaccgcgcc catgccgaag
     3061 gcgagcgcga acaagaagac atacgcgcgg atcaggtctt cgggtccgac gccgccgaac
     3121 acgaagacga gactcgccag cgggatgctg gcgatgatca acagaaagac gtaggtcagc
     3181 gcagagaaga gcttgccgat aaccatcccc agggagctca ggggtgtcgt caccagcatg
     3241 tcgagcgtct gcttctcgcg ttcgagcgaa attgcgcccg tcgtgaaagc tggagcgagc
     3301 acgaggacca agagggtttc gagaaagagg agcccaccga agatgccgtg gccgatcgcc
     3361 gcggagaggg ccgtgccgtt ggataacgtc tgctggttcg gcagcggaaa gcccgggaag
     3421 tcgcgcggga tgccgggatt gactccgatg gttgacgtgg tcagggtcgc ctgctgcttc
     3481 aggtagacgt agattgcgaa cgcgaacaac gcgagcagga ggaggtagac cgtcagcacc
     3541 acgaaggcac gcctgccgcg catgcgaccg cgcagctcct tgatgctgac tgcgctgata
     3601 cccgaccaga aacggcccag ccggctgccg atgaaacggc cgccgcgccg gactcccgcc
     3661 accgctgttg tcacgcttgc acctcagtcg accttggctg acctgcgttg ctggacgctg
     3721 cgtgggcgtc ggtgatctga aggaagagct cctcgaggtc gctcgcggct ggaccgaagc
     3781 ttgccagcct gacgccggag cggatcgccg acgtgagcac tccggaggcc gcgtcggcgt
     3841 cgccacgcaa ggcgaactcc agctcgccgg ttgggcgcgt cacgacggac gccacctgcg
     3901 ggtcggcggc gaaatgccgc tgggccgcag cgaacgcagc ttcgtcgccg acgaccgttg
     3961 cgcggtagac gccgccgacc cggaaccgct cggcgatgtc gtccacgcgg ccactcgcca
     4021 ggacgcggcc gtggtcgatg atcgctacgg aggtgcagag ctcctccagc tcgggcagga
     4081 tgtggctgct gatcacaatg gtcttgccca aggagcgcag ctcgcgcagg agctcgcgga
     4141 gctcgactcg agcgcgtgga tcgagccctg aagcgggttc gtcgagcagc agcacggacg
     4201 ggtcgtgtac gagtgcgtgg gcgaggcaca gccgttgctg catgccgcga ctcagctcct
     4261 ggacgtaagc gctgcgcttg tcggcgaggt ccacgagctc aagcaggtca ccgatcatct
     4321 gccgtcgccg ctggctggag agcccatagc agcggccaaa gaaatcgagg tactcccaga
     4381 cgcgcatgtc gtcgtagacg ccgaaggtgt caggcatgta accgatcacg ttgcgaactg
     4441 cgtcaggatc gcgccgtatc gaatgcccaa cgatctctgc gtcgcccgca gacggcggca
     4501 gcagagtggc cagcatcttg agtgtcgtcg tcttgccggc gccgttgggg ccgaccaggc
     4561 cgaagatctc tccctgctca acctcgagct ccacgccggc gacggccagc gaccgcccgt
     4621 agcgcttggt caaccgttca gtcctcaggg caatggtcat tcaatcgtcc cctcgaggcg
     4681 gaccagcagg ttgaaatact tctggtcctc gccgaactgg ccggggtcgt tgctgttgac
     4741 gaagcggaac tgcacccggc cgctctcgtc gacgtagcgc tcggggtttg agatgcggta
     4801 gctctgcgtc atgctcggat gcgggaactg aacccatagg ccgctcgttc gatcgaacag
     4861 ctggtagtcc ggcatggggt ccaagcccac cgcgcccgga tcaacgggcg gacggcccgg
     4921 ttcggcctca cccttcccgt cggcaggggc cgccgtcggg gctggggcgg ggcactcgac
     4981 cccgggtggg caggtcgtct ccggcgtcgg tgacggctcc agatcgctga gcgggtcgtc
     5041 ctggggtggc tgctgctcag cgggcagtgg ctccacgaac tcgccggtgc cacgcaggac
     5101 gcgcgtctca ccctgggtca gcgcgatctc gagcgcggtc gcggcaaacg ccccatcgaa
     5161 gcgagccggg tggacctcga ccgtcatcgt gccccgggag agatacatgc tcgaggcgtc
     5221 agcccacgct gtggcagcgc tggaatcaac gatgtcgcgc cggagcatct ggtcggcaaa
     5281 gatctgatcg gagctgatcg ccatggcgag cgggaccatg tacaggccgt ccccgacccg
     5341 gttcggctcg tcacccggca actcgaccgc gagcactggg cccttgctcc aggcgagcag
     5401 taggggagcg tcggcagcgc cctggctgcc ccccgggaag agttggtcga gcaccgatcg
     5461 acgcgtgctt atggtccgtg ccgtcgcgga gtcgcgcggg aacgtggtcc cgaagatggc
     5521 ttccgagagg gcgtagccga agaacggact ggtggtgaca tcgagctcga cctctttgct
     5581 ctgggccggc tcaaggtcgg acaagatcgc ggcgccgccg ctgaagacga tggcaacgtt
     5641 ctcgagcttc atgagggacc ggttggtgac cgtgccggtg agcttgccgc gctcgagg
//
LOCUS       DATLCO010000122         3186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6034208, whole genome shotgun sequence.
ACCESSION   DATLCO010000122 DATLCO010000000
VERSION     DATLCO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6034208"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1643)
                     /locus_tag="VMZ33_03000"
     CDS             complement(138..1643)
                     /locus_tag="VMZ33_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HUR16231.1"
                     /translation="MSDLSSTPEWVSDAVFYQIFPDRFSMSSRVAKPGPLEPWDAPPT
                     FNGFKGGDLLGIAEQLDYLTDLGVNALYLNPIFSSASNHRYHTYDYFQVDPLLGGNDA
                     FRELLDTCHARGVRVVLDGVFNHSGRGFWPFHHVVETGEGSPYVDWFYVDRQTLHSKG
                     LRPYPTAEESRRMSEVAGPNRTGSDSKRELGYQAWWDLPALPKLNTNNPQMREHLMRA
                     SEFWVEFGIDGWRLDVAEEIDADFWREWRRRVRAVNPDAFITAEIWREKPEWVTGDTF
                     DSMMNYPLTEALLSFTAARYLDNRVVSEQHEYRDFVRPIDGVGFASRLEHIMSMYRPE
                     AIRTQLNLLDSHDTPRYVTVAGGDRDSYRLASLAIFTLPGAPCVYYGSEVGVEGSHDP
                     DCRRAFPWDESRWDAGLRSFVRALAQTRHDVKDLRRGAFKVVAAAGDAVAYERGNAVV
                     VINSGEETARLSIPGAGLSGKRVAELPGWGPLRVAADGSVSVDRRSGGILL"
     gene            complement(1640..>3186)
                     /locus_tag="VMZ33_03005"
     CDS             complement(1640..>3186)
                     /locus_tag="VMZ33_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HUR16232.1"
                     /translation="ALHPPRIRYRFGLETQADGLRWFGADGLRDEPRPRGAFEFAYLA
                     EGDRTVSPDWARGAVFYHIFPDRFARGSGAPARRGLANWDDVPTSESFLGGDLDGITE
                     HLDHIASLGVDALYLTPIFSAPSNHKYDTADYFAVDPAFGGDEALRRLIGALHERGMK
                     LVLDGVFNHVGRTMPQFADVLARTDASPYRSWFYFDGDKYETWSTDVPALPKLRTSEP
                     AVRDLVCRIGRHWVEQFGIDGWRLDVANEVDHALWRAFRASVREANADAYLVGEVWDL
                     AMPWLRGDQFDAVMNYPARSAILRYAGAGPGGVAFPLEEPLDGEAFLNAVDRVRGAYP
                     EPIHDLLYNLVGSHDEVRPLFALREDRAAAAVAAALLFTLPGIASIYYGDEVGMSGGN
                     DPLNRAGMIWDEPRQDAQMLALYRRLGRLRTDIPALRSGSYRRLAEDGALAAFARETA
                     EGRAVVVANASAKAVRVLPSEVSSWLEEKSGRLEILSHASVSRSKWSRGDLSLPAQSV
                     AVVVAE"
BASE COUNT          584 a         1096 c         1036 g          470 t
ORIGIN      
        1 ccctgccgcg atgccgccca ccgcgagacc tgaaaccacc aagggcagcc gggtggccga
       61 cgtgtcgacc agcagcccgt acagggccac ggcacccgcg ccgaccatga gcaggaccaa
      121 catcacccga gccagagtca cagcagtatt ccccctgaac gccgatccac gctgacagag
      181 ccgtcggccg caacgcgcaa cggtccccac ccgggtagct ccgcgacgcg cttgccggag
      241 aggcccgccc caggaatcga cagccgcgcc gtctcctccc ccgagttgat gactacgacc
      301 gcgtttccac gctcgtacgc aaccgcgtcg ccggccgcgg cgacgacctt gaacgccccg
      361 cgccgcaggt ctttgacatc gtgtcgcgtc tgcgccaagg cacggacgaa cgagcgcagc
      421 ccggcgtccc agcgcgattc gtcccacggg aaggcgcgcc ggcagtcggg atcgtgcgaa
      481 ccctccacgc ccacctcaga cccgtagtag acacacggcg cccccggcag cgtgaagatc
      541 gccagcgacg ccaaccgata agagtcgcga tccccgcccg cgacggtcac atagcgcggc
      601 gtgtcgtgcg agtcgagcag gttgagctgc gtccggatgg cctcgggccg atacatggac
      661 atgatgtgct ccaaccgtga cgcgaagccc acgccgtcaa tgggtcggac gaaatcccgg
      721 tactcgtgct gctccgacac gacgcgatta tccagatagc gcgccgcggt gaacgagagc
      781 agcgcctcag tcagcgggta gttcatcatc gagtcgaaag tgtcgccggt aacccactcc
      841 ggcttctcgc gccagatctc agccgtgatg aacgcgtccg gattgacggc acggacgcgg
      901 cgacgccact cgcgccagaa gtcggcgtcg atctcctcgg cgacgtcaag ccgccagccg
      961 tcgatcccga actccaccca gaactccgac gcgcgcatca ggtgctcgcg catctgcggg
     1021 ttattggtgt tcagcttcgg cagcgccggc aggtcccacc acgcctggta gccgagctcg
     1081 cgcttcgagt cactgcccgt ccggttgggt ccggccacct cgctcatccg acgcgactcc
     1141 tcggctgtgg ggtacggacg aaggcccttg gagtgcaagg tctggcgatc gacgtagaac
     1201 cagtcaacgt acggcgagcc ctcgcccgtc tcgacgacgt ggtggaatgg ccaaaagccg
     1261 cggcccgagt ggttgaacac tccatcgagg acgacgcgga ccccccgcgc gtgacacgtg
     1321 tcgagcaact cgcggaatgc gtcgttgccg ccgaggagcg gatcaacctg gaagtagtcg
     1381 tacgtgtggt agcggtgatt ggacgccgac gagaagatgg gattgaggta gagcgcgttc
     1441 acgccgaggt ccgtgaggta atcgagttgc tcggcgatcc ccagcaggtc gccgcccttg
     1501 aacccattga aggtcggtgg cgcgtcccat ggctccagcg gcccgggctt agctacgcgc
     1561 gaactcatcg agaagcggtc cggaaagatc tggtagaaga ccgcgtccga cacccactcg
     1621 ggcgtcgagg aaaggtcgct cattcggcga ccacgacagc gaccgactgc gccggcagtg
     1681 aaaggtcacc ccttgaccac ttcgaccgac tgacggaggc gtgcgagagg atttcgagcc
     1741 ggcccgattt ctcttccagc cagctcgata cctcggaggg cagcacgcgg accgctttgg
     1801 cggaggcgtt ggcgacgacc accgcccgac cctcggccgt ctcgcgcgca aacgccgcaa
     1861 gcgcaccgtc ctcggccagc cggcgataac tgcctgatcg cagtgccggg atgtccgtac
     1921 gcagtcggcc gagacgtcgg tacagggcga gcatctgggc gtcctggcgc ggctcgtccc
     1981 agatcatgcc tgcgcgattg agcggatcat ttccgccgga catgcccact tcgtctccgt
     2041 agtagatcga cgcgataccc gggagggtga agagcagcgc tgccgccacg gcggccgcgg
     2101 cgcgatcctc gcgcaacgcg aacagcggcc tgacttcgtc gtggctcccg accaggttgt
     2161 agagcaggtc gtggatcggc tcaggatatg cgcccctcac gcggtcgaca gcgttgagaa
     2221 acgcctcgcc gtccagtggc tcttcgagcg gaaacgcaac gcctccaggg cccgccccgg
     2281 cgtaacgcag gatcgccgag cgggccgggt agttcatcac cgcgtcgaac tggtcgccgc
     2341 gcaaccacgg catcgccagg tcccacacct cgccgacgag atacgcgtca gcattcgcct
     2401 cgcgtacgga cgcgcggaag gcgcgccaga gcgcgtggtc gacctcgttg gcgacgtcga
     2461 gccgccagcc gtcgattcca aactgctcga cccaatggcg gccgatacga cacacgaggt
     2521 cgcgcacggc cggttcgctc gttcgcagct tcggcagggc tggcacgtcc gtcgaccacg
     2581 tctcgtactt gtcgccgtcg aagtagaacc agctgcggta cggagaggca tccgtgcgcg
     2641 ccaggacatc cgcgaactgc ggcatggtcc ggccgacatg gttgaacacg ccgtcgagga
     2701 cgagcttcat gccgcgctcg tggagtgcgc cgatcaaccg gcggagcgcc tcgtcgccgc
     2761 cgaacgccgg atcgaccgcg aagtagtcgg ctgtgtcgta tttgtggttg gacggcgcgc
     2821 tgaagatcgg agtcaggtac agcgcgtcga cgccaagaga ggcgatgtgg tcgagatgct
     2881 cggtgatccc gtcgaggtcg ccaccgagga acgattcgga ggtcggcacg tcgtcccagt
     2941 tcgccagccc acgacgcgcg ggcgcgcccg agccgcgagc gaatcggtcg ggaaagatgt
     3001 ggtagaaaac cgcacctcgt gcccaatcgg gcgagacggt cctgtcgccc tccgccaggt
     3061 acgcgaactc gaacgcaccg cgcggacggg gctcgtcgcg cagaccgtcc gcgccgaacc
     3121 agcgcaggcc gtctgcctga gtctccaatc cgaagcgata acggatacgg ggcgggtgca
     3181 gtgcca
//
LOCUS       DATLCO010000123         7628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9760801, whole genome shotgun sequence.
ACCESSION   DATLCO010000123 DATLCO010000000
VERSION     DATLCO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9760801"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VMZ33_03010"
     CDS             complement(<1..914)
                     /locus_tag="VMZ33_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16233.1"
                     /translation="MTVRSWSAAALIALVLVGCGTSAPGPSPTPVNEETVWQRAISQI
                     GDDGRYTLEAALNLFATAYGPLPGVNVEQNFGGEEVVDRNIAIAAVMSHAAELTSEQR
                     AAIDGYLAPPPDADVITVPPVSVSTGRVAMVGIPDQLAHGVAEPFAALGADTKKAYED
                     AAKGFRSQIAAKLGRDFSGDLKVFFKDEPGPLTKYGGRAAADAWSDWPGGVFGDCHIR
                     IFQGGQQGEPIEILGTLAHETFHCFQLDAFRTIDSYGIEPPWVVEGQATWVEATLTEG
                     SGCCAAPWDQWLGREDVLTKRAYSAIGF"
     gene            complement(911..1306)
                     /locus_tag="VMZ33_03015"
     CDS             complement(911..1306)
                     /locus_tag="VMZ33_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HUR16234.1"
                     /translation="MSTSEAIELIAAHRQAFADAMRRGDSAAAASVYAVNAHLIAPSA
                     GPMVGRDEIRSFWQAGIDAGVAEVSLMANGVEQNESLAYETGAYLFLVNPADAGRVVE
                     RGHYVQVYQRQADGSWQRAVEIFSPGGSE"
     gene            complement(1354..4227)
                     /locus_tag="VMZ33_03020"
     CDS             complement(1354..4227)
                     /locus_tag="VMZ33_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HUR16235.1"
                     /translation="MSEEGLPGSVATGPSHRVEFRVLGPVEALKNGQPVALGGPRQRT
                     LLALLLLRPGRTVPVDELLDAIWAGEPPEGAATTIRSYVSRLRAALGDGAIVHASASG
                     YSLSVDPQAIDAAQFDRLVRTADDDLSSGDARAAAERLTRAADLWRGRAFGNLSDDGP
                     LRIEAERLDELRLHAVEQRFEAALALGRAGEVVDEIEAETTLHPYRERLWRLLMLALY
                     HSGRQADALATFKRARKLLDDQLGIDPSPDLQALEGQILRQELAPLGHADRRSNSPSP
                     QTSFVGRANELAELAIRLRENRLLTLTGVGGVGKTRLALELARSAGDLASDGVVFVDL
                     APLPQAGDVADAVARTLELREQPGASSTDLVASELRHMRLILVLDNCEHVRAAAAGVA
                     DVILARCPEVRVVATSRMSLGVDGELEFPLAPMAVPAEHATADEMRASDAVRLLVERV
                     AASRPRGFDDDQALAEAARICRDLDGLPLAIELAAARAKVMSLGEIAGRLNDRFRFLV
                     SWRRMSAARHQTLKQAIDWSYDLLAAEDQRMFAALAVFSGGFTLDAVAAISSSGDDLT
                     ALDAVTRLVDASLVVAEPSETEIGTRYRMLETVRQYAVHRLRESGRTDALQDTHAEFY
                     LRLAEQAEPELSGAGQTAWFTRLDDEHANFVTALDHLSRRAHAGSDAQSLLAFTVALT
                     RFWYVRGHLTAAREALQRALNQSGSAPILLRRRGLTAAASIALLQGDYPEATRYAEQS
                     LAAARETKEERLVANGLSNLGAIVLAGGDHERAGTLLHEAVGLARSVGDTRILALALN
                     NLGDHALTIGEYDRAEPLFAESLALLEERGDTANVARSLFNLGAVALMTARLDAADER
                     LRGSLARSRDAGDKEDLSWSLLGLAGLAAAKAQHERAALLLGAATNLMAGMGADFKPF
                     ERKLHDETAAQVSERLGTERYGHTLAKGRALSLADAIEVAAQA"
     gene            complement(4309..6105)
                     /locus_tag="VMZ33_03025"
     CDS             complement(4309..6105)
                     /locus_tag="VMZ33_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16236.1"
                     /translation="MKQAGTLIAICLLVGLAGPSPAAAAEAPSASSFGDTGLSTFGPR
                     SGTFDPRLVVEADGSFVGTAGLAGTFDEGGWTLGSDLASGEPPILEPGGASVTATAVV
                     GTVYALAVSGNNLYVGGEFQNVAGIPEADYIARWDGTAWSALGSNGDGNGALDAGVRA
                     IAIHGSNVVIGGFFTDVAGIAGADFVARWTGSSWAPLQNLCCGAAAINNSVHALAVGG
                     DFVYAGGYFTDVAGFTGVNYLARWSVPCDCWSSVGLAGSMTGPVFAIAPGRSRIYVGG
                     LFSDAGGNASADNVAFLSHGEWSSLGNGVNGPVFTMLVYEPNEFDEFVYVGGGFTDVG
                     GATGDYLARFDSLDWYPVGSNGAGDGALNDSVYALTLVNGSLFVGGTFEDAGGNVNAD
                     SGARWSGSAWFPLAAGFSALNSHVFALAASSNGLYIGGHFTDAGGQAEADRVVLRKGS
                     EWIPLSPRRPDGRIRLRNGAFVGNNVYNGTGVNQTRTGLAAPGQVITFGISAQNDSAV
                     PDRIRVGATGTSVSGYTIKYFTGGTDVTLAVNSGTYQTPVLPAGGSVLITARVTVKQS
                     AAGGSSISRLVTLRSVADQTKLDVLKLVAKRT"
     gene            complement(6225..7427)
                     /locus_tag="VMZ33_03030"
     CDS             complement(6225..7427)
                     /locus_tag="VMZ33_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUR16237.1"
                     /translation="MSFEPPTPAIDARAKQVSRNADMRGVAEALVAQRAAILDGWQNV
                     ATRQPFHAGHPDRAIADHIPALFDALTDLLSRDIPGEDIEAPMDDAAVAAAARAHAQM
                     RFEQSLGPVAIATEFRLLRQEISRALRVQLEEDLSIDDVVSSLAVVNDALDGATNLAL
                     SALTDRIETVREEFLATTLHDVRQPIVLIQASLVLAARWARREPLDLVQLTETLDGAL
                     VATQEMSHLVETLADASRAAMGALDLIPEPVVVREIIKDALDTLDATGRRRVTVDSSL
                     ASGAVVYWDRQSIRRVLTNLLTNALKYSPPESPVTVTVTQDVANIEVTVRDHGMGLLP
                     DEKAILFQRYSRTAEARTRGLPGVGLGLFACKGLVEAHGGRIALESDGRDQGTTARIL
                     LPIGKPDS"
BASE COUNT         1308 a         2578 c         2473 g         1269 t
ORIGIN      
        1 tagaacccga tagccgagta cgcacgcttc gtcagcacgt cctcgcgccc gagccattgg
       61 tcccacggtg ccgcgcagca gccggagccc tcggtcagcg tcgcctccac ccaggtcgcc
      121 tgtccctcga cgacccacgg cggctcgatg ccgtacgagt cgattgtgcg gaacgcgtcg
      181 agctggaagc agtggaaagt ctcgtgcgca agcgtcccga ggatctcgat gggctccccc
      241 tgctgacctc cctggaagat ccggatatgg caatcgccga aaacgccacc cggccaatcg
      301 ctccacgcgt ctgctgctgc gcgaccgcca tacttcgtca gggggccggg ctcgtccttg
      361 aagaacacct tgagatcgcc gctgaaatcc cggccgagct tcgcggcgat ctggctgcga
      421 aaacctttcg cggcgtcctc atatgccttc ttggtgtcgg cgcccagcgc agcaaagggc
      481 tcggcgacgc cgtgcgcgag ctggtcggga atgcccacca tcgcgacgcg gccggtggac
      541 acgctgaccg gcggaaccgt gattacgtcc gcatcgggcg gcggcgccag atagccgtct
      601 atcgccgccc gctgctcgga cgtgagctca gcggcatggc tcatcaccgc cgcgatcgcg
      661 atgttccggt cgacaacctc ttcacccccg aagttctgct cgacgttgac gccggggagt
      721 ggcccgtatg ccgtggcgaa caggttgagc gcggcctcaa gggtgtatcg cccatcgtcg
      781 ccgatctgcg agatcgcccg ctgccagaca gtctcctcat taacgggagt aggcgacggc
      841 cctggcgcgg atgttccgca accgaccaac acgagcgcga tgagagctgc tgccgaccag
      901 ctacggacag tcattccgaa cctcccgggc tgaagatctc cacggctcgc tgccacgacc
      961 catcggcctg gcgttgatat acctgcacgt aatgaccccg ctcgacgaca cggccggcgt
     1021 cggcgggatt gaccaggaac aggtaggcac cggtctcgta tgcgaggctt tcgttctgct
     1081 caaccccgtt ggccatcagc gacacctcgg caacgccagc gtcgataccg gcctgccaga
     1141 agctgcggat ctcgtcgcgg ccgaccatcg gcccggccga cggcgcgatc aggtgcgcat
     1201 tcactgcgta gacggaggcc gcagctgctg agtcgccacg acgcatcgca tcggcgaacg
     1261 cctggcggtg tgcggcgatc aactcgatgg cctccgatgt cgacatctcg acgagattgg
     1321 ccgagttcgg ttgcaatccg gttgcaggcc gcctcaggct tgggctgcca cctcgattgc
     1381 gtcggccagc gacagcgcgc gtcccttggc gagcgtatgt ccatagcgct cggtgccgag
     1441 tcgctctgac acctgtgcgg cggtttcgtc gtgaagcttg cgctcgaacg gcttgaagtc
     1501 agcgcccatg cccgccatca ggttggtcgc ggctcccagc agaagtgcgg ctcgctcgtg
     1561 ctgagccttg gcggccgcca gtccggcaag accaagaagg ctccacgata ggtcctcctt
     1621 gtcgcccgca tctcggcttc gcgccaggct gccgcgcagg cgttcatcgg ccgcgtcgag
     1681 tcgagcggtc atcagtgcca ctgcgccgag attgaacagc gagcgcgcca cgttcgccgt
     1741 atcgcctcgt tcctcaagaa gggccagact ctcggcgaac agcggctccg cccggtcgta
     1801 ctcgccgatg gtcagggcgt ggtcgccgag gttgttgagg gcgagggcca ggatgcgcgt
     1861 gtcgcccacg ctccgcgcca gacctacagc ctcgtggagc agagttccgg cacgttcgtg
     1921 atcgccaccg gcaaggacga ttgcgccgag gttgctgagg ccgttcgcaa cgaggcgctc
     1981 ctcctttgtc tcacgggcgg cagcaaggct ctgctcggcg taacgggttg cttcggggta
     2041 gtcgccctgg agtagcgcga ttgacgccgc ggccgtcagc ccacgccggc ggagcaggat
     2101 cggagccgag cccgactggt tcagagcacg ctgaagcgcc tctcgtgccg ccgtgaggtg
     2161 gccccggacg taccagaatc gggtgagcgc gacagtgaac gccagaagcg actgagcgtc
     2221 cgatccggcg tgcgcccggc gggacagatg gtcgagcgct gtgacgaaat tggcgtgctc
     2281 gtcatccaga cgggtgaacc aggccgtctg gcctgcgccc gacagctccg gctcggcttg
     2341 ctcagccagc cgcagataga actccgcgtg cgtgtcctga agagcgtcgg tgcgcccgct
     2401 ctcgcgcagc ctgtgaacgg cgtattgccg aaccgtctcc agcattcggt agcgcgtgcc
     2461 aatttctgtt tcggagggct cagcaaccac gagtgaggca tcaacgagac gcgtcacggc
     2521 atcgagcgcg gtcaagtcgt cgccactact actgatcgct gccaccgcgt cgagcgtgaa
     2581 gccgcctgag aagaccgcca aagcggcaaa catgcgctgg tcttcggcag ccagcaggtc
     2641 gtagctccag tcgatcgcct gcttgagcgt ctggtggcgc gccgccgaca tccgacgcca
     2701 cgacacaagg aagcggaagc gatcgttgag gcgtccagcg atctcgccca gcgacatcac
     2761 cttggctcgg gccgcagcca gctcgatggc gagcggcagc ccgtcgagat cgcggcagat
     2821 tcgcgctgcc tcggccaggg cctgatcgtc gtcaaagcca cgcggccgcg acgccgcaac
     2881 ccgctcaacg aggagccgga cggcgtccga cgcgcgcatc tcatcggccg tggcgtgttc
     2941 cgctggaaca gccatcgggg cgaggggaaa ctcgagctcg ccgtcgacgc ccagtgacat
     3001 gcggctggtg gccacgaccc gaacctctgg acagcgggcc aggatcacat cggcgacacc
     3061 ggccgcagcc gcacgcacgt gctcgcagtt gtccaggacg aggatcaaac gcatatgtcg
     3121 cagttcgctc gcgacgagat ccgtcgagct ggctcccggc tgctcacgaa gctccagagt
     3181 ccgtgcaacg gcatctgcga catcgcctgc ctggggcagg ggcgccagat cgacgaacac
     3241 gacaccgtca ctggccagat caccggcgct ccgtgcgagc tcgagcgcaa ggcgcgtctt
     3301 gcccacgcct cccacaccgg tcagggtcag caggcggttc tcccgcaatc gaatggccag
     3361 ctccgcgagc tcgttggccc ggccgacgaa gcttgtctgc gggctgggtg aattgctcct
     3421 gcggtcggcg tgcccgagcg gcgccagctc ctgtcgcagg atctggccct cgagtgcctg
     3481 gaggtctggg ctgggatcga tgcccagctg atcgtcgagc agcttgcgcg cgcgcttgaa
     3541 cgttgccaac gcgtcggcct gacggcccga gtggtagagc gccagcatca gcagccgcca
     3601 gagtcgttca cggtacgggt ggagagttgt ctctgcctcg atctcgtcga cgacttcgcc
     3661 cgcccgtccg agagccagcg ccgcttcgaa ccgttgctcg accgcgtgta gacggagctc
     3721 atccagtcgc tcggcctcta tgcgcagcgg gccgtcatcg ctcaggtttc cgaacgcccg
     3781 gccgcgccac aggtctgcgg ctcgcgtgag ccgctcggca gcggcacgcg catcgccgct
     3841 ggacaggtcg tcgtccgcgg ttctgaccag gcgatcgaac tgagcggcgt caatcgcctg
     3901 ggggtcgacc gagagtgaat agccggacgc gcttgcgtga acgatcgcgc cgtcgcccag
     3961 ggccgctcgc agacgcgaaa cataggaccg gatggtggtc gccgcaccct ctggcggctc
     4021 gccggcccag atcgcgtcca acagctcgtc gaccggcacg gttcggccgg ggcgcagcag
     4081 aagaagcgcc agtagcgtcc gctgtcgcgg gccgccgagc gccaccggtt ggccgttctt
     4141 cagcgcctcc accgggccca gcacgcggaa ctcgactcgg tggctcggtc ccgtagcaac
     4201 cgagccgggg aggccctcct cgctcacgag agcgaagcct aagggtcacg ccaaatcagc
     4261 gaccccgttc gcctcgtgtc ctggctgcgt cggcagaaat gtggccggtc aggtccgttt
     4321 tgcgaccagc ttgaggacgt cgagcttggt ctgatcagcg accgagcgca gggtcacgag
     4381 ccggctgata ctcgaaccgc cggcggcact ctgcttgacc gtcaccctgg cggtaataag
     4441 gacgcttccg cctgctggta gcacgggcgt ctgataggtg ccagagttga cggccagggt
     4501 cacgtcggtg cccccggtga agtacttgat tgtgtaaccg ctgacactgg tccctgtcgc
     4561 cccaaccctg atgcggtctg ggacagcgct gtcgttctgc gccgagattc caaacgtgat
     4621 cacctggccg ggggccgcca agccggtcct ggtttggttt acgcctgtgc cgttgtagac
     4681 gttgttgccg acgaatgcgc cgttcctcag gcggattcgg ccgtccggcc tgcgaggcga
     4741 caacggaatc cattccgagc cttttcgaag gacgacccgg tctgcctctg cttggccacc
     4801 cgcgtccgtg aagtgcccgc cgatatagag gccgttgcta ctcgccgcga gcgcgaacac
     4861 gtgcgagttc agcgccgaaa aacctgcggc cagcgggaac cacgcgctcc cactccagcg
     4921 cgcacccgaa tcggcgttca cgtttccccc ggcgtcctcg aacgtgcctc caacgaacag
     4981 cgatccgttg acgagggtca gtgcatagac cgagtcgttc agcgccccgt cgccggcacc
     5041 gttggacccg accggatacc aatcgaggct gtcgaaccgg gcgaggtagt cgccggtcgc
     5101 tcctccgaca tcggtaaagc ctccgcctac gtagacgaac tcgtcgaact cgttcggctc
     5161 gtacacaagc atcgtgaaga caggtccgtt gaccccgttg cccaacgacg accattcgcc
     5221 gtgactcaag aaagcgacgt tgtcggcgga agcgttgcca ccggcgtcgc taaagagacc
     5281 gcccacgtag atccgggacc ggcccggagc aattgcaaaa actggtccgg tcatagagcc
     5341 ggccagacca accgaggacc agcaatcgca cggcaccgac cagcgagcga gatagttcac
     5401 gcccgtgaac ccggccacat cagtgaagta gccgccggcg tacacaaagt cgccgccgac
     5461 cgcgagtgcg tggaccgagt tgttgatggc cgcagccccg cagcacaggt tctgcaacgg
     5521 cgcccagctg ctgccggtcc accgcgcaac gaagtcggct cccgcgattc ctgcgacgtc
     5581 cgtgaagaag ccgccgatga cgacattcga accgtgtatt gcaatggcac ggacgcctgc
     5641 atcaagagcg ccgttaccgt ctccgttcga acccagagcc gaccaggccg tgccgtccca
     5701 ccgagcgatg tagtcggcct ccgggatgcc ggcgacgttc tggaactcgc cgccgacgta
     5761 cagattgttg ccgctcaccg ccagcgcgta gacggtacct actactgctg tcgccgtaac
     5821 tgacgcgcca cctggctcga ggatgggcgg ctcgccgctg gcaaggtcgc tgccgagcgt
     5881 ccacccgccc tcatcgaagg tgccggcaag cccggctgtg ccgacgaagc tgccgtctgc
     5941 ttctaccacc agcctcggat cgaatgtgcc cgacctgggg ccgaacgtgc tcaagcccgt
     6001 gtcgccaaaa gaactggccg atggcgcttc ggctgccgcg gcaggcgatg gcccggccag
     6061 accgaccaag aggcagatcg cgattaaggt tccagcctgc ttcatgctca gtctcccgtc
     6121 gtgcgatcgc acgacgcggt gccagccgcg cgcgtcgaac ctgagcatgc tcgttcccag
     6181 cggttgcaat ccggttgcag ccgcgtggcg ctgagagtgg agcgtcagct atccggcttg
     6241 ccgatcggca gcaggatgcg agcggttgtg ccttgatcgc ggccatccga ttcgagtgcg
     6301 atccggccgc cgtgcgcttc gaccagaccc ttacaggcga acagccccag accgacgccc
     6361 ggcagcccgc gggtgcgagc ctctgccgtt cgactgtagc gctggaacag tatggctttc
     6421 tcatcgggca gcaggcccat cccgtgatcg cggacggtga cctcgatatt cgcgacatcc
     6481 tgtgtgacag tgacggtcac gggcgactcg gggggcgagt acttgagtgc attcgtcagg
     6541 aggttggtta ggacacgcct gattgactgt cgatcccagt acacgacggc gccgctggcg
     6601 agcgacgaat cgaccgtgac tcggcggcgg ccggtggcat cgagcgtgtc cagtgcatcc
     6661 ttgatgatct cgcggacgac caccggttct ggaatcaggt ccagcgcacc catcgccgcg
     6721 cgcgatgcat ctgccaacgt ttcgacgagg tgcgacatct cctgagtcgc gaccagagca
     6781 ccgtcgagcg tttcggtcag ttgcacaagg tccagcggtt cacgccgtgc ccagcgtgcc
     6841 gccaacacga gcgaggcctg gatgaggacg attggctggc gcacgtcgtg aagcgtcgtc
     6901 gccagaaatt cctcgcggac agtctcgatc cggtcggtca gggccgacag cgccagattg
     6961 gtggcgccgt cgagggcgtc gttcaccacg gccaacgagc tcacgacgtc atcaatcgac
     7021 aggtcttcct cgagctggac ccgcagcgcg cgagatattt cctggcgcag gagccggaac
     7081 tccgtcgcga ttgccacggg gccaagactc tgctcgaacc tcatctgagc gtgcgcccgt
     7141 gcggctgctg ctaccgcggc atcgtccatc ggcgcctcga tgtcctcgcc gggtatgtcc
     7201 cgcgaaagca ggtcggtcaa ggcatcgaag agagccggaa tgtggtcggc gatagcccga
     7261 tcaggatgcc ccgcgtgaaa cggctgtcgc gtcgcgacgt tctgccaacc gtcaagaatg
     7321 gcagcgcgct gtgccacgag ggcctccgca acgccacgca tatcggcgtt ccgtgagacc
     7381 tgcttggctc gcgcgtcgat tgccggcgtc ggtggctcga acgacatgca cggctccttg
     7441 ttcgagacag acaggcgagt taggggcggc ggctgatgat aacgccgccg ggcgaccgcg
     7501 aggcgctgcg ccgccgggga cttgggcacc ctaaggacgc gccgcggagc ggatgtcctc
     7561 cagcgagagt gggttttcga gcgccgagag gtcacccggg tcggttccaa gatatgccgc
     7621 gcgcgcaa
//
LOCUS       DATLCO010000124        10789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1635213, whole genome shotgun sequence.
ACCESSION   DATLCO010000124 DATLCO010000000
VERSION     DATLCO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635213"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VMZ33_03035"
     CDS             complement(<1..960)
                     /locus_tag="VMZ33_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUR16238.1"
                     /translation="MTAQRTVETNTFMFTDIEGSTRLLQELGDRYPQVLDDQRRLIST
                     AVENHGGRVFGSEGDALFAAFPSAVGAVAAAAESQRSLAGHAWPSVTPLRVRMGIHTG
                     EAMAAGDNFVGLALHEVARIMAAGHGGQVLVSEATRRVAQSLPHGLELRDLGERRLKD
                     LAAPERLYQLVSEGLADKFPPLRTLDARNNNLPVQVTSFIGRAELAAARAALAETRLL
                     TLTGPGGTGKTRLALQLAGDVSDQFTDGVFFVPLDAITDPMLLASQIASSIGLTLSGT
                     GSPADAVIGYLRDRQALLLLDNFEQIIEAATEVGRLVREAPGIK"
     gene            complement(981..1580)
                     /locus_tag="VMZ33_03040"
     CDS             complement(981..1580)
                     /locus_tag="VMZ33_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16239.1"
                     /translation="MRRVAVLALMVVLSAAACGEARTTVRPSQSLLNVEFTPRDSPTI
                     PVETADSTDEPTFVSIPVGWDNAFCGIVTDAVDAQELVIDVESALEDEDFKGARGLAR
                     DLRDVTEDASALLADLPDWDEAADAVLELATLVDLGTRAGAEYGTYFAEPDQNRTALR
                     RARELRNQIKRATPAANEALAALEGLGIVCDVPLHLETF"
     gene            complement(1577..2278)
                     /locus_tag="VMZ33_03045"
     CDS             complement(1577..2278)
                     /locus_tag="VMZ33_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16240.1"
                     /translation="MAYNRRPRRQGVVYGPPRPEGTSENGVLFGRFLGLGLIALTLGL
                     LGVGIVAFLDRQNPSATPGRSAVVAASVSFLPPTIAPSGATTLPPATPTPLVTPTGET
                     PTPTGPAPSTTPLPVQEGPGFVTFGTQADEELRITDPRTTFTMQEQVVWSAYLTRPAD
                     SSTLRIQIAKADDSTPNGERQVVDAEVTPEVVDGQIFQRRFRPNRLLDGAGVYIVRYV
                     AGTEILAQGTIQVTR"
     gene            2425..2562
                     /locus_tag="VMZ33_03050"
     CDS             2425..2562
                     /locus_tag="VMZ33_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16241.1"
                     /translation="MEQHRAARARRDSAQLGSDDFRAAAEEIARIEVEIARREEPPAP
                     G"
     gene            complement(2585..3508)
                     /locus_tag="VMZ33_03055"
     CDS             complement(2585..3508)
                     /locus_tag="VMZ33_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16242.1"
                     /translation="MEGSNTPIEHRFPSNGADPVATAAALWQGPSDPQMRVGNGRVAR
                     ALRTAHGVATVLLERTSDRAEIVARAWGEPAAAEAALALVPGLSGALDDPSALLPRHD
                     LVAGLVRRLPGLRLTRTGSVMDALVLSIFAQKVTGLEAHRAWSGLLRRYGDGAPGPFG
                     LFAPPPPEKIAKLPYWAFHPFGVERRRADTIRAAAAVARQLEAIRDLPSPDGTRRLMS
                     LSNIGVWTAAETMRFALGDPDAVSVGDYHLPRVICTALAGEPRGDDARMLELLEPYRG
                     QRARVALLIERGGFARERHGPRMTVRSIATI"
     gene            complement(3586..4926)
                     /locus_tag="VMZ33_03060"
     CDS             complement(3586..4926)
                     /locus_tag="VMZ33_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16243.1"
                     /translation="MPLAAFGFLLLPGPVAAHTLTERYQAPLPLIAYVAGAALAVAMS
                     FVFVMLRGATPAIALAEARSRTVPAWLRGLLSASGLIAWLWIVVQALGNGQGSGDVAS
                     LFLWVYGWVGVALVCALVGPAWAWLDPFSTIHRILGAIGSRIGLSGSEPADYPGRFGQ
                     WPAVVGFALIVWIELVARIEGGRTLGLLLVAYTLISVAGMSYFGRDTWRRNAELFSVW
                     FGILGRLAPFALEDEPEDGRVVRRSFASGLLAQRWTVAELVIVALGTGSIIFDGLSQT
                     QIYFDLFGGVSILGLPTVRDTLILGAFLGLLVTVVLIVARRLSVDALGAGLLPVAVGY
                     LVAHYFTFLLVDGQRIINAINDPLLSGANLLPPQFAFYEPSLPLSPAITWSIQLAAVV
                     GGHVIGAWAGHAALAEDEPDAPMHRQLPLATLMVVLTSVTLWSLGQAVLSGPAS"
     gene            complement(4965..5870)
                     /locus_tag="VMZ33_03065"
     CDS             complement(4965..5870)
                     /locus_tag="VMZ33_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HUR16244.1"
                     /translation="MKIGVIVPQGWTGEYTGWDPRTAWARARDVAWQAEALGFESIWL
                     FDHFHTVPRPTFEITFEAFTTLSALAAETHRVRLGHVVLCAAYRNPALTAKMISTLDV
                     ISGGRMELGIGAGWKKDEWLAYGYGFPDAPERLAILKDQLQVITAMMGKGPAEFHGNH
                     HSVERAINRPLPLQEPRVPIMVGGNGRNVTWRLAAKYADELNLDGVPVDEIDESMDVI
                     RQRCEEIDRDPSSLTVSVHVWWESVPEHGQQSVDMLGAYAEKGVSRIIAMIRDSVSSD
                     EALAHWADDARAAGIEMFDSTAIGS"
     gene            5950..6288
                     /locus_tag="VMZ33_03070"
     CDS             5950..6288
                     /locus_tag="VMZ33_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16245.1"
                     /translation="MATIRQVDVAMLDKPKASRAAPRELTPKQRERLEQQRQFEKMLS
                     RLTDKDTVFEVRLDSSEKPLTVRQRLMKAASTINQEIVVRKSEKGWLVGMATPERRSK
                     RGRRKSNPLA"
     gene            6299..6667
                     /locus_tag="VMZ33_03075"
     CDS             6299..6667
                     /locus_tag="VMZ33_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HUR16246.1"
                     /translation="MKEPAELQVQIFGRRDSRDTQRALRFFAERRVKVAFVDVARKPP
                     ALGELRRFAERFGSKALMDSESRLYRDAGLAYLSLDDGQVLERVLADPRLLKLPLVRA
                     GSRVTIGVDDVAWKSWLVRT"
     gene            complement(6664..8397)
                     /locus_tag="VMZ33_03080"
     CDS             complement(6664..8397)
                     /locus_tag="VMZ33_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16247.1"
                     /translation="MPQHRRATWLVIAALTATFCFSAPAATVVAGVGDARCTNQTDAL
                     HPPAYIRVQRLSSGHSGEVDQVPFWNYVGTVLRGEYGTGVRRSPHWLRVGAMIVKQYA
                     WYYVVKWRGSHNAAGECFHVKDTTADQMYKPEFYTPNSTNLSAMRDTWHMALRKWVPD
                     KSQSTIFLTSYRTGLRKPCGADAIGHRIYQKSLRDCENKNLVLEEMLRKYFEPNLQIV
                     DTRGRDAVEDNGAFNGDLVTFADAGNATAWRTYAGSNGSWANGTNGEFEVHVSSVLGQ
                     GVGNMDTTRNNTTEPLADVILLVDNGSGRELRVHRATGNGFIQTPDTKVVDGNASKLL
                     VADFNGDQRVDAGLLTQNGSQATLNVILLNRERPDNTFEKFVPASEWWSGNLDLSTTQ
                     QLAGDTDGDGKADLVVRQADGSFAIAHSDPSCSTYSEWGPCPTASIASNHLGSLENAL
                     SSAAAPATSAKLALTDFNRDGRDDVVAVRHNGSDNVTVFGMRGKGDGTLMDPIQLWQG
                     TPMIPFANLRVVGMNVNSDSLGDVALVSLGDATKPVWLRTSEQTKTSPPLMTLVPSND
                     ALTWSTASHAF"
     gene            8542..9783
                     /locus_tag="VMZ33_03085"
     CDS             8542..9783
                     /locus_tag="VMZ33_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HUR16248.1"
                     /translation="MNAQAPLRRVPASPVSAAEVAQVRLPVDQARLLPPRVFHDPAVF
                     EFEQDRWFASTWLCAGRVEDVAEPGMYLLVQVAGEGVIVVRGRDHELRAFNNVCRHRG
                     STLVDEPLDGKNRLVRIQCPYHAWVYELDGTLKRAPHTDEIVDFDAADNGLLRVRVDV
                     WEGFVFVNLDGDAPPLREYLADLPAAVAAYPIGATRRARRIEYEVNANWKVIGENYSE
                     CYHCPGVHPQLNRLSPYDRGQNLESNGPWTGGWMELIDAADTMSTDGASHGRPPLAGI
                     GDDNLRRVYYVVLWPNLLLSLHPDYVMTHQVWPIDAERSRVVCEWHFDPQVMARADFD
                     PSDAVDFWDLTNRQDWEVCERQQTGTRSRAYVAGRYSLMEDMVHAFDLMVADRYADDG
                     VDTRFAPRHDKWSDTPRRRAG"
     gene            complement(9801..10310)
                     /locus_tag="VMZ33_03090"
     CDS             complement(9801..10310)
                     /locus_tag="VMZ33_03090"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUR16249.1"
                     /translation="MFRGSRGGLPRLDLRTTRTIRSAIEIRAPRDAVWTVLTDFASYP
                     EWNPHIRQVRGEPRVGRRLTIRSQPPSGRPVVLRPVVEVFDPPAELRWRSTFMARRLF
                     SGEHGFKLESIADGRTRFMQDETVTGLLVPLYARLRLPSARQGFAEVNEALRTRAENA
                     TSERGGAAA"
     gene            complement(10415..>10789)
                     /locus_tag="VMZ33_03095"
     CDS             complement(10415..>10789)
                     /locus_tag="VMZ33_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR16250.1"
                     /translation="MTRFSRGDRDFLLAHTPLGLCLTDDRCPHMAAPLSIGTLTNCVV
                     ACPLHRGTFDLASGDVVQFPTTGGLDAHGNYHGPWTPAGAPSKDEPTDLKARARALTR
                     IRRMRYYSLRIVGDMIEAQLPG"
BASE COUNT         1872 a         3512 c         3581 g         1824 t
ORIGIN      
        1 cttgatgccc ggcgcttccc gaacgagtcg cccgacctct gttgcggcct cgatgatctg
       61 ctcgaagttg tcgagcagga gcagcgcctg tcgatctcgg agatagccga tcacggcgtc
      121 agccggcgag ccagtaccgg acagagtcag gccgatggac gaggcgatct gggaagcgag
      181 gagcatgggg tcggtgatgg cgtccagggg aacgaaaaag acgccgtccg tgaactggtc
      241 gctgacgtct ccggccagct gtagggccag ccgcgtcttt ccggttccgc cgggaccggt
      301 gagcgtcagc aggcgcgtct cggcgagcgc ggcgcgcgcc gcagccagct cggcgcgacc
      361 gatgaagctc gtgacctgca cgggcaagtt gttgtttcgc gcgtcgaggg tgcgcagcgg
      421 cgggaacttg tccgcgagtc cctcactcac gagctgatac agccgctcgg gcgccgccag
      481 atccttgagc cgccgctcgc caaggtcgcg cagctccagg ccatggggca acgactgggc
      541 cacgcgacga gtcgcctccg agacgagaac ctggccaccg tgaccggccg ccatgattcg
      601 tgccacctcg tgcaacgcca gtcccacgaa gttgtcgccg gcagccatcg cctcgcccgt
      661 gtgtatgccc atgcgcacgc gtagcggcgt caccgacggc cacgcgtgcc cagcaaggga
      721 gcgctgcgac tccgcggccg ctgccaccgc gccgacggcg ctgggaaatg cggcaaatag
      781 cgcatcgcct tccgacccga agacccgacc gccgtggttc tcgacggcag tcgagatcaa
      841 ccggcgctga tcgtcgagca cctgcggata tcggtcgccc agctcctgga gcagtctggt
      901 cgagccctcg atgtcggtga acatgaaggt gttcgtctca acggtgcgct gggcggtcat
      961 ggcggtttgg tgccgacgct ctagaacgtc tccaggtgga gcggcacgtc acatacgatg
     1021 cccagcccct cgagcgcggc gagcgcctcg ttcgccgctg gcgtagcgcg cttgatctgg
     1081 ttacgcagtt cacgcgccct gcggagcgcg gtgcgattct ggtccggctc tgcgaagtag
     1141 gtgccgtact cggcgccagc gcgggtgcct aagtccacga gtgtggccag ctcgagcacg
     1201 gcgtcggcag cctcatccca atcgggcagg tctgccagga gggcacttgc gtcctccgtc
     1261 acgtcgcgca ggtcgcgcgc gagcccgcgg gcgcccttga agtcctcgtc ctcgagcgca
     1321 ctctcgacgt cgatcaccag ttcctgggcg tctacggcat cggtaactat gccgcagaag
     1381 gcgttgtccc agccgaccgg gatgctcacg aatgtcggtt cgtcggttga atcggcggtt
     1441 tcaaccggga tcgttgggct gtcgcgggga gtgaactcca cattgagcaa agactggctg
     1501 ggccggaccg tcgtgcgtgc ctcaccgcag gccgcggcgg aaaggacgac catcaatgcc
     1561 aacacggcaa cacgcctcat cgcgtcacct ggatcgtgcc ctgcgcgagg atctcggtgc
     1621 cggcgacata gcggacgatg tacacgcccg cgccgtcgag aaggcggttc ggccggaagc
     1681 gccgctggaa gatctgtcca tcgacgactt ccggggtcac ttctgcgtca accacctgcc
     1741 gctcaccgtt cggggtcgag tcgtccgcct tggcgatctg aatccgaagc gtggacgaat
     1801 cagccggtcg agtcagatag gcgctccaca ccacttgctc ctgcatggtg aaggtcgttc
     1861 gcggatcagt gatgcgcagc tcctcgtcgg cttgagtccc gaaggttacg aagcccgggc
     1921 cttcctgcac aggcagtggc gtggtgctgg gcgcaggccc ggttggcgtt ggtgtctcgc
     1981 cggtgggtgt caccagcggg gtcggggtgg ctggtggaag agtagtcgcc ccggatggtg
     2041 cgatggtcgg tggcaggaag ctcaccgaag cggcgacgac ggcgctacgt cccggcgttg
     2101 ccgacggatt ctggcggtcg aggaaggcca cgatgcccac cccgagcagt cccagggtca
     2161 gggcaatcag ccccaaaccg agaaaacggc caaaaagaac gccgttctcg ctggtcccct
     2221 cggggcgagg cggtccgtag acaacgccct ggcgtcgcgg tcggcggttg taggccacgg
     2281 acggattgtt gcaaatcgtg atgtagagtc gagaagatga gctctgagcg acaaactacg
     2341 ggtgctggga ctgccggttc ccgcccttca acaggcgccg gagccggcgc tggaactggc
     2401 gccgtgaatg gtcgaggcgc cctgatggaa cagcaccgcg cggcgcgggc ccgtcgtgac
     2461 tcagcacagt tggggagcga cgacttcagg gcggctgccg aggagatagc ccggatcgag
     2521 gtcgagatcg cccgacgcga ggagccgccc gccccgggct agcgcagcct cgccgtccgc
     2581 gccgtcagat cgtcgcgatc gaccgcaccg tcattcgtgg cccgtgcctc tcccgtgcga
     2641 agccgccgcg ctcgatgagc agggccactc gcgcccgctg acctcgatac ggttccagta
     2701 gctcgagcat ccgagcgtcg tcgcccctcg gctcgcccgc cagcgccgtg cagatgacac
     2761 gtggcaggtg gtaatcgcca acgctgactg catccgggtc gcccagcgcg aaccgcatcg
     2821 tctcggctgc ggtccagaca ccgatgttcg acagcgacat gagcctcctg gtcccgtctg
     2881 ggctggggag gtcacggatg gcttccagct ggcgtgccac ggccgcagca gcgcggatgg
     2941 tgtcggctcg tcgtcgctct accccgaacg ggtggaaggc ccaatacgga agctttgcga
     3001 tcttctcagg tggcggtggc gcgaagaggc cgaaaggtcc gggcgctccg tcaccgtatc
     3061 gacgcagcag tccgctccag gctcggtgcg cctccaggcc cgtcaccttc tgggcaaata
     3121 tcgacaggac gagtgcgtcc atgacgcttc cggtccgggt caggcggaga cccggcaaac
     3181 ggcggaccag tccggcgacg aggtcgtgcc gcggcaggag ggccgatggg tcgtccaacg
     3241 ctccagacag tcccggtacg agtgcaaggg ccgcctccgc cgcggcgggc tcgccccacg
     3301 cacgtgcgac gatctcggcc cggtccgagg tccgttcgag gagcaccgtc gccacgccgt
     3361 gcgctgtccg cagggcacgc gccactcggc cgttgccgac ccgcatctgc gggtcgctcg
     3421 gtccttgcca gagggctgcc gcagtagcca ctgggtcggc gccattcgac gggaacctgt
     3481 gctcgatcgg cgtgttggac ccctccatcg tcggagtcta cgaagtgccg cggccctgcg
     3541 cgcgggctgt actcacaaga agcctggcgc cagcgaggat cggctctagc tcgcgggccc
     3601 gctcaacact gcctggccga gtgaccacag cgtcaccgaa gtcagcacga ccatcagcgt
     3661 cgccagcggc agctgccggt gcatcggggc gtcgggctca tcttcagcca atgccgcgtg
     3721 cccggcccac gctccgatga cgtggccgcc gacgaccgcc gcgagctgga tgctccacgt
     3781 gatcgcaggg gagagtggca gcgacggctc gtagaacgca aactgcggcg gtagcaggtt
     3841 cgcaccgctc aacagtggat cgttgatggc gttgatgatg cgctggccgt cgacgagaag
     3901 gaacgtgaag tagtgggcta cgaggtagcc cacggcgacg ggtagcagac cggcgccgag
     3961 cgcatccacg ctgagccggc gggcgactat gagaaccact gtcacgagca ggcccagaaa
     4021 cgcgcccagg atcagggtgt cacggaccgt gggtaggccg aggatgctga ccccgccaaa
     4081 taggtcgaag tagatctgcg tctggctgag tccgtcgaag ataattgagc ccgttccgag
     4141 agcgacgatc acgagctcgg ccacagtcca gcgttgggcg agcaggccgc tggcgaaaga
     4201 tcgcctgacg acgcggccgt cctcgggctc gtcctcaagc gcgaacggcg cgagtcggcc
     4261 caggatgccg aaccagacgc tgaataactc cgcgttccgg cgccacgtgt cgcgcccgaa
     4321 atacgacatc ccagccacgc tgatgagcgt gtaggcgacg agaagcagcc cgagcgtccg
     4381 tccaccctcg atacgcgcca cgagctcgat ccagacgatc agggcgaagc ctacgaccgc
     4441 gggccactga ccaaatcgcc ccggatagtc ggccggctca ctgcctgaca gacctatacg
     4501 gctgccgatg gcgccgagaa tgcgatggat ggtcgagaac gggtccagcc atgcccaggc
     4561 tgggccgacg agcgcgcaga caagagcgac ccctacccag ccgtagaccc acaagaagag
     4621 cgacgcgacg tcgccactgc cctggccgtt gccgagagcc tggacaacga tccacagcca
     4681 cgcgatgagt cccgaggctg acagcaggcc gcgcagccat gccgggacag ttcgagagcg
     4741 agcttcagca agcgcgatgg cgggcgtcgc accgcgcagc atcacgaaga caaacgacat
     4801 cgcaacggcg agcgcggcgc cggccacgta cgcgatgagg gggagtggcg cctggtagcg
     4861 ctcggtcagc gtatgggcgg cgaccgggcc tggaaggagc aggaacccga aggcggccag
     4921 aggcatcgcc cgagccagaa gcaaccgggc gtgcatggcg gaggtcagga cccgatcgcc
     4981 gtgctgtcga acatctcgat gccggccgca cgggcgtcat cagcccaatg ggcgagagcc
     5041 tcgtcgctgc tgactgagtc gcgaatcatt gcgatgatgc gcgaaacgcc cttttccgcg
     5101 tacgcgccga gcatgtcgac actctgctgt ccgtgctcgg gcacggactc ccaccacacg
     5161 tgaactgaca ccgtcaacga cgacgggtcg cggtcgatct cctcgcatcg ctgccggatc
     5221 acgtccatcg attcgtctat ctcgtcgacc ggcaccccgt cgaggttcaa ctcgtcggcg
     5281 tatttcgcag cgagtcgcca cgtgacgttg cggccgttgc cgccgaccat gattggcact
     5341 cgcggttcct gcagtggcaa tggccgattg atagcgcgct caacggaatg atgatttccg
     5401 tgaaattcag ctgggccctt gcccatcatt gccgtaatca cttggagttg gtctttgaga
     5461 atcgcgaggc gttcaggcgc atcggggaag ccgtagccgt acgccagcca ctcgtccttc
     5521 ttccatccgg ctcctattcc gagttccatc cgccccccgc tgatcacgtc gagcgtggaa
     5581 atcatctttg cggtcaacgc cgggttgcga taggcagcgc acaggacgac gtgccccaag
     5641 cgcacgcgat gagtctccgc agcgagggcc gacaaagtcg tgaaggcctc gaaggtgatc
     5701 tcgaatgtgg gccgtggaac ggtatggaaa tgatcaaaca gccagatcga ctcgaagccg
     5761 agcgcctcag cctgccaggc aacatctcgt gctcgagccc aggcggtcct ggggtcccag
     5821 cccgtgtatt cgccggtcca gccctgcgga acgatgacgc ctattttcat gacaagcgct
     5881 cctttacatg acgggccgat ctatgtcacg atttcggcgc cgtgagtgtt aattcagagg
     5941 tgtattgaaa tggcaacgat ccgacaggta gatgtggcga tgctcgataa gccgaaggca
     6001 tcgcgggctg cgcccaggga gttgactccc aagcagcgcg agcgcctcga acagcagcgc
     6061 cagttcgaga agatgctatc tcggctcacg gacaaggaca ccgttttcga agtccggctc
     6121 gattcgtctg agaagcccct gaccgtccgt cagcgactca tgaaggcggc gtcgaccatc
     6181 aaccaggaga tcgtcgtccg caagtctgag aaggggtggc tggtcgggat ggcgacgccg
     6241 gagcgccgct ccaagcgcgg tcgccgcaag tccaacccgt tggcctgagt cgcggccggt
     6301 gaaggaaccg gctgaactcc aggttcagat cttcggacga cgcgactcgc gcgacactca
     6361 gcgcgcactg cgcttcttcg cggagcggcg cgtcaaggtt gcattcgtcg atgtcgcccg
     6421 caagccaccg gccctcggag agctacgacg ctttgccgag aggttcgggt cgaaggcgct
     6481 gatggacagc gagtcgcgcc tctaccgcga cgccggcctg gcctatctct ccctggatga
     6541 cggccaggtg ctcgaacgtg ttctcgccga cccgcggctg ttgaagctgc cgctcgtgcg
     6601 cgcgggatcg cgcgtgacga tcggcgtgga cgacgtcgcc tggaaaagct ggctcgtccg
     6661 cacctagaac gcgtggctgg cggtactcca cgtcagcgcg tcgttgctcg ggacgagcgt
     6721 cataaggggc gggctcgttt tcgtctgctc tgacgtcctg agccagacgg gtttggtggc
     6781 gtcaccaagc gataccaaag cgacgtcacc gagggagtcg gagttgacgt tcatcccgac
     6841 gacccgaagg tttgcgaacg gaatcattgg cgtgccctgc cagagctgga tcggatccat
     6901 cagcgttccg tcacccttgc cacgcatgcc aaagacggtc acgttgtcgc tgccgttgtg
     6961 gcggaccgcg acgacgtcat cgcggccatc gcggttgaag tcggtgagcg ccagttttgc
     7021 cgacgtagcc ggcgcagcgg ccgaggacaa ggcgttctcg agtgagccta gatggtttga
     7081 tgcgatcgac gcagtcgggc agggccccca ctccgagtac gtagagcagc tcgggtctga
     7141 gtgcgcgatg gcgaacgagc cgtccgcctg gcggacgacg agatccgcct tgccgtcgcc
     7201 gtcggtgtca cccgcgagct gctgggtggt actcaggtcg agattgccac tccaccactc
     7261 actggccgga acaaacttct cgaacgtgtt gtctggtctt tcgcggttga gaaggatcac
     7321 gttgagcgtg gcctgcgagc cgttctgagt caggaggcca gcgtcgacgc gctgatcacc
     7381 gttgaagtcg gccacgagga gcttcgaagc attcccgtcg accacctttg tgtcgggcgt
     7441 ctgaatgaag ccgttgcccg ttgcgcgatg aacgcgaagc tcgcggccgc tgccgttgtc
     7501 gacaagcagg atgacgtcag cgagcggctc cgtcgtgttg ttcctggtgg tatccatgtt
     7561 gccgacgccc tgaccaagga cgctgctgac atggacctca aactcgccgt tcgtgccatt
     7621 tgcccacgag ccgttgctcc ccgcgtatgt gcgccacgcc gtcgcgttgc cagcgtcggc
     7681 gaacgtgacc aggtcgccgt tgaaggcgcc gttgtcctcg acggcatcac ggccgcgcgt
     7741 gtcgacgatc tgtaggttcg gctcgaagta cttgcgcagc atctcctcga gaacgaggtt
     7801 cttgttctca caatcgcgga ggcttttctg gtagatgcga tggccgatgg cgtcggcacc
     7861 gcatggcttc cttaggccgg tccggtagct cgtgagaaaa atggtgctct ggctcttatc
     7921 gggcacccac ttgcgcagcg ccatgtgcca tgtgtcgcgc atcgctgaaa ggttcgtgct
     7981 gttgggcgtg tagaactccg gcttgtacat ctggtcggcc gttgtgtcct tgacgtgaaa
     8041 gcactcgccc gctgcgttat gcgagccacg ccacttcacg acgtagtacc acgcgtattg
     8101 cttaacgatc attgcgccga cgcgcagcca gtgcggggag cgacggaccc cggtgccgta
     8161 ctcacctcgc agcaccgtgc cgacatagtt ccagaagggg acctggtcaa cttccccgct
     8221 gtgaccgctg ctcaaacgct ggacgcggat gtacgccggc ggatgcagcg catccgtctg
     8281 gttcgtacag cgcgcatcac cgacaccagc tacgacggtc gcagctggag cggaaaaaca
     8341 gaaggtcgcg gtcaacgcgg caatgacgag ccacgttgcg cggcggtgct ggggcacgtg
     8401 acgcaattta caaatccctc ggtgcttggt cgatcagccg atgggtcctc cccgggggga
     8461 ccatcggtcg aaatggattg cggacggcgc cggtcgtgtg cagtacgcca acacggctcg
     8521 tggcgtacgc tgagcgcccc catgaatgct caggcgccgc tgcggcgcgt accggccagt
     8581 ccagtaagtg ccgcggaagt tgcccaggtg cggctgcccg tcgatcaggc gcgcctgctc
     8641 ccgccgcgtg tcttccacga tccggccgtc ttcgagttcg agcaggaccg ctggttcgcg
     8701 tcgacgtggc tctgcgcggg ccgtgtcgag gacgtcgccg agccgggcat gtacctgctg
     8761 gtccaggtcg cgggggaggg cgtcatcgtc gtccgtggcc gcgaccacga gctgcgtgcc
     8821 ttcaacaacg tttgccggca ccgcggctcc acgcttgtcg acgagccgct cgacggcaag
     8881 aaccgcctcg tgcgcatcca gtgcccgtat cacgcctggg tgtacgagct tgacggcacg
     8941 ctcaagcgag cgccccacac cgacgagatc gtcgatttcg acgccgcgga taacggcctg
     9001 ctccgcgtgc gtgtcgacgt ctgggaaggg ttcgtgttcg tcaatctcga cggcgacgcg
     9061 ccgccgctgc gggaatacct cgccgatctg cccgcggccg tggctgcgta cccaattggc
     9121 gccactcgcc gcgctcgccg catcgagtac gaggtcaacg ccaactggaa ggtcatcggc
     9181 gagaactact ccgagtgcta tcactgcccc ggagtgcatc cgcagctcaa ccggctgagc
     9241 ccctacgacc gcggccagaa tcttgagtcg aacggcccct ggaccggcgg ttggatggag
     9301 ctcatcgacg cggctgacac gatgtccacg gacggcgcca gccacggtcg gccgccgctc
     9361 gccggcatcg gtgacgacaa tttgcgccgc gtctactacg tcgtcctgtg gccgaacctg
     9421 ctgctcagcc tccaccctga ctacgtgatg acccaccagg tctggccgat cgacgccgag
     9481 cggtcgcgcg ttgtgtgcga atggcatttc gacccgcagg tcatggcgcg ggccgacttc
     9541 gatcccagcg acgccgtcga cttctgggac ctcaccaacc gacaggactg ggaggtctgc
     9601 gagcggcagc agaccggcac tcgatcgcgc gcctacgtgg ccgggcgtta ctccctcatg
     9661 gaagacatgg tccacgcgtt cgacctgatg gtcgccgacc gctacgccga cgacggcgtc
     9721 gacacgcgtt ttgcgccgcg acacgacaag tggtcggaca cgccgcgccg tcgcgccggc
     9781 tgaagtaggt cggcgctcca tcacgcggct gcgccgccac gctcgctcgt ggcattctcg
     9841 gcgcgcgtgc gcagcgcctc attgacctcg gcaaaaccct gtcgcgcgct gggcagccgc
     9901 agccgtgcat agaggggtac gagcaagccg gtaaccgtct cgtcctgcat gaagcgcgtc
     9961 cgaccatcgg cgatggactc gagcttgaat ccgtgttcgc cgctgaacaa acggcgtgcc
    10021 atgaacgtcg agcgccagcg tagctcggcc ggcgggtcga agacctccac gaccggtcgg
    10081 agcaccactg gcctaccgct cggtggctgg ctgcggatcg tcaatcttcg gcccacgcgc
    10141 ggttccccgc gaacctgacg aatgtgcggg ttccattccg ggtaggaggc gaagtccgtc
    10201 aagaccgtcc acacggcgtc gcgcggagct cttatttcga tggcgctgcg aatcgttcgc
    10261 gtcgtgcgca ggtccaagcg cggcaacccc cctcggcttc cacgaaacac acggcaccct
    10321 ctgaagtaga agcccggttc gaggtcaagt ttaggggact gaggtgccgt tagcgaatcg
    10381 ctggcgaccc gtttggcccg gtgtcaggac tggttcagcc gggtagctgc gcctcgatca
    10441 tgtcaccgac gatccgcaat gagtagtagc gcatgcgccg gatccgcgtc agcgctcggg
    10501 cccgtgcctt gaggtcggtt ggctcgtcct ttgacggcgc tccggcaggc gtccacggtc
    10561 cgtgatagtt cccatgggca tccaagcccc cggtcgtcgg aaactggacg acgtcgccac
    10621 tcgcgaggtc aaacgtgcca cggtgcagcg ggcacgccac gacgcagttg gtcagggtgc
    10681 cgatcgacag cggcgcggcc atgtgcgggc agcgatcgtc ggtcaggcac aggccgagcg
    10741 gcgtgtgggc cagcaagaag tcgcggtcgc cgcgcgagaa acgagtcat
//
LOCUS       DATLCO010000125         3236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_147205, whole genome shotgun sequence.
ACCESSION   DATLCO010000125 DATLCO010000000
VERSION     DATLCO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147205"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..665)
                     /locus_tag="VMZ33_03100"
     CDS             complement(147..665)
                     /locus_tag="VMZ33_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUR16251.1"
                     /translation="MTDPIDDSDLSPAVLAARDQIQNPRLLSDRIAMGRALWRDLVVG
                     FASDLAQLNLGFSQLAALYAADGMPTLTVADLAERIGRSPSATSRLVSELERRGLLRR
                     HEEVADRRQRTLEVTAEGRQMLARLDSARADEFLAIVRPLPPSERALVAMGVAALSSH
                     AITRRGRLIKSR"
     gene            728..1699
                     /gene="fabD"
                     /locus_tag="VMZ33_03105"
     CDS             728..1699
                     /gene="fabD"
                     /locus_tag="VMZ33_03105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HUR16252.1"
                     /translation="MSQLAIVFPGQGSQYLGMGEALTQSSSAAAAVFAEADAALGESL
                     SQLCFSGPEDDLNLTVNSQPAILATSIAYTYALEERLGRLPEVSFYAGHSMGQYSAMV
                     AAGVLSLSDGIRLVRERGRQMQASASDGAMAAIIGLDEARLPELLDAGAALGVFTLAN
                     RNSPGQIVVSGERAAVEGAATVARELGAKRALVLMVSVAAHSPLMSLAAERMGVALES
                     VSFSEPRAPLLANSDARLLTTGEECRAELVEHLTRGVDWIAAVNHMTAAGVARFVEPG
                     PGKVLTGLIKRIAPEAETYPLDDQSSPGRLATPEFLSPTVDPTGATA"
     gene            1702..2970
                     /locus_tag="VMZ33_03110"
     CDS             1702..2970
                     /locus_tag="VMZ33_03110"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HUR16253.1"
                     /translation="MPMRRPDFSRRVAVTGLGIISPVGQDVDSAWDNLTHGRSGLKRI
                     TRWDPTPYEAQVAGEIEFDGTQWMDFKAIRRTDKNVVMGVAAAKQALADSGLVVDESN
                     RDDIGVIFGSGGGGPLLLMENTEAWRNKGARTVSPFFIANMLPDTASGQIAIDTGIRG
                     SNMCVVTACSTGTHNIGEAAEGIRRGDYVAALTGATENPLHELVHIGFSNMRGMGLPR
                     PDEGLETVSRPFDRTRNGFVLGEGAGAMMLEDLEFAKARGAKVYAEVVGYGSAADAWD
                     LIQPIEQGDGARRAMLQALERHGVPANEIDLINPHGTSTPLGDLREAQAIWAAFGDHT
                     PNIAISATKSMTGHLMGAAGAIEGVFTVLSVYNQCAPATLNYRDEDPEINLNVVAGAS
                     RSMEIRYAMSDNIGLGGHNGAVIFKRYDGD"
BASE COUNT          563 a         1078 c         1079 g          516 t
ORIGIN      
        1 tgcccatgct cgcctatcag ccggaggagc gaccgacggc caaggaagtc gccgcacggt
       61 tagaggacct cgttgaggag gcgcggctgg tcgcccgcaa gcgtcttcac gcccggcacc
      121 gccggtgagc gacgccagcc ggcggactaa cgagacttga tcagccgccc gcggcgagtg
      181 atggcgtgac tggagagagc ggcgacaccc atcgccacga gcgcacgctc gctcggcggc
      241 agcggccgca cgatggccag aaactcgtcg gctcgggcac tgtccagacg ggcgagcatc
      301 tgccgaccct cggcagtcac ctcgagcgtt cgctggcgac gatcggcaac ttcctcgtga
      361 cgccgcagca atccccgccg ctccagctct gagacgagcc gactcgtcgc cgacggcgag
      421 cgaccgatcc gctccgccag gtcggcgact gtcagcgtcg gcatgccgtc agcggcgtac
      481 agcgccgcca gctgcgagaa gccgaggttc agctgagcca ggtcggacgc aaacccgaca
      541 acgaggtcgc gccacagggc tcgccccatg gcgatccggt cggagagaag ccgcgggttc
      601 tgaatctggt cgcgcgcggc gagtaccgca gggctcaggt cactgtcatc aatcgggtcc
      661 gtcattatgt gcatgattgc acctaatcgg gcggtcggaa cgttaatcgc tgataacgtg
      721 cctgctcgtg tcacagcttg ccatcgtctt tcccggtcag ggctcccagt acctgggcat
      781 gggtgaggcg cttacccagt catcatccgc agccgcggcc gtcttcgccg aggccgacgc
      841 tgcactgggc gagtccctct cgcagctctg cttcagcggg cccgaggacg acctcaacct
      901 caccgtcaac tcgcagccgg cgatcctggc gacgtcgatt gcctacacgt acgcgctcga
      961 ggagcgtctc ggccggttgc ccgaggtgag cttctacgcc ggccattcca tgggccaata
     1021 cagcgcgatg gtcgcggccg gtgtcctgtc gctgagcgac ggcatccggc tggtccgcga
     1081 acgcggaagg cagatgcagg cgtcggcctc ggacggggca atggcggcca tcatcggcct
     1141 cgatgaggca cgtctgccgg agcttctgga cgcgggcgcg gcgctgggcg tcttcaccct
     1201 tgcgaaccgc aattcgccgg ggcagatcgt ggtcagcggc gaacgcgccg cggtcgaggg
     1261 tgccgcaact gttgcccggg agctcggcgc caagcgggcg ctcgtgctga tggtcagcgt
     1321 cgccgcgcac tcgccactca tgtcgcttgc cgccgagcgg atgggcgtcg ctcttgagtc
     1381 cgtctccttt tctgaaccgc gcgcgccact gctggcgaac tccgatgcgc gactgctgac
     1441 taccggcgag gaatgccgcg ccgagctcgt cgagcacctc acccgcggcg tcgactggat
     1501 cgcggccgtg aatcacatga cggccgcggg tgtcgcccgc ttcgttgagc cggggccagg
     1561 caaagtcctg actggcctga tcaagcgcat cgcgcccgaa gccgagacct acccgctgga
     1621 cgaccaatcg tcacccggtc gtctcgcgac tcccgagttc ctgagcccaa ccgtcgaccc
     1681 cactggagct accgcctagc catgcccatg cgccgccccg atttttcgcg tcgagttgcc
     1741 gtcaccggcc tcggcatcat cagcccagtc ggccaggacg tcgacagcgc gtgggacaac
     1801 ctgacccacg gtcggtcggg cctcaagagg atcacccgct gggatcccac gccgtacgag
     1861 gcgcaggtgg ccggcgagat tgaattcgac ggcacgcagt ggatggactt caaggccatc
     1921 cgacgcaccg acaagaacgt cgtcatgggt gttgccgcgg ccaagcaggc gctcgccgac
     1981 tcgggcctgg tggtggatga atcgaaccgt gacgacatcg gcgtgatctt cgggtcaggc
     2041 ggtggcggcc ctcttctgct catggagaac accgaggctt ggcgcaataa gggcgcgcga
     2101 accgtctcgc cgttcttcat cgcgaacatg ctgcccgaca cagcgtccgg ccagatcgcg
     2161 atcgacaccg gcatccgcgg ctcaaacatg tgcgtggtca cggcctgctc gacgggcacg
     2221 cacaacatcg gggaggcggc agaggggatc cggcgcggtg actacgttgc ggcgctgacc
     2281 ggcgcgacag agaacccgct gcacgagctg gtgcacatcg gcttttcaaa tatgcgcggc
     2341 atgggcctgc cgcggcccga cgaggggttg gagaccgtgt cgcggccgtt cgacaggaca
     2401 cgcaacggct ttgtgctggg cgaaggtgcc ggggcgatga tgctcgagga tctggagttc
     2461 gccaaggccc gtggcgcgaa ggtgtacgcc gaggtcgtcg gctacggctc agccgcggac
     2521 gcgtgggacc tcatccagcc gatcgagcag ggcgatggcg cacgccgcgc gatgctccag
     2581 gctttggagc gtcatggcgt gccggccaac gagatcgacc tgatcaaccc gcacggaact
     2641 tcgacaccac tgggcgacct gcgggaggcg caggcgatct gggccgcgtt cggcgaccac
     2701 accccgaaca tcgccatctc ggcgaccaag tctatgaccg gccatctcat gggcgcggcc
     2761 ggggcgatcg agggcgtgtt caccgttctg tcggtctaca accagtgcgc cccggcgacg
     2821 ctcaactacc gcgatgagga tcccgagatc aacctgaacg tggtggccgg cgcgtcgcgt
     2881 tcgatggaga tccgatatgc gatgagcgac aacatcggtc tgggcggcca caacggcgcg
     2941 gtgatcttca agcgctacga cggcgactga gcacggcggc cgagcccttc tatcgcagca
     3001 tcgatatgca gcgcatgact gccgatgcgg gtgtcagatc aatagcgacc tgcggaggtc
     3061 gccgaatgtg aattgccggc gctctaatcg tgctaacccc gcacaattta gtcatgcggc
     3121 gcatgcgcgg ggagacatct gacgtcgaaa tgactcgatg gggcggtcgg atacgtgcag
     3181 cccgagtcct agccccaacc caaagcgctc aggtaagcat gcactgcata cggcag
//
LOCUS       DATLCO010000126         5283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2385245, whole genome shotgun sequence.
ACCESSION   DATLCO010000126 DATLCO010000000
VERSION     DATLCO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385245"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VMZ33_03115"
     CDS             <1..702
                     /locus_tag="VMZ33_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16254.1"
                     /translation="RWYRLRDQESGWRKRAAVNGLGAATTAVVAVEVAVTKFFHGAWV
                     VLILIPLLIAMMLFIKRQYASSAAQLAVKPGVKLPGPHRDERAIVPVSGLNRAVVQAV
                     NVGRSIADDVLAVLVSDNPEDAARLRERWEKQLPDVPLIIVESPYRTIVGPLLAYLDV
                     LDQMWPAGKEQPITFVVIPEFVARHWWERLLYNQSAARLRRALLGRAHTVVVNVPYRR
                     EETASSVSNVSEPPG"
     gene            complement(764..1429)
                     /locus_tag="VMZ33_03120"
     CDS             complement(764..1429)
                     /locus_tag="VMZ33_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUR16255.1"
                     /translation="MNISIIGSGNIAKALGVSAVRAGHQVVIASRNNDSAAATASEIN
                     ATAAESLADAVAAADVVIFAVPATAAFDVAREIAPVIAHEVVIDVTNPLKGDMSGLFT
                     EGTSIAEELAGILGDAKLVKGFNTLFGSVQANPQTHGTTLDALFAIDDDTARETFATL
                     AMSLGFRPVHVGPLAAARELESMALLNIRLQIVSNGYWQSSFVLVGAPESSTATPVLA
                     KAA"
     gene            1570..2133
                     /locus_tag="VMZ33_03125"
     CDS             1570..2133
                     /locus_tag="VMZ33_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR16256.1"
                     /translation="MGADRPRPPIGREVRHWRRARGLTLGQVGERSQLNIGYLSQIEN
                     EKALPSLDALMAIAGALDVPAAWLMIDSSQPPRVVRATERPTVATSDAGGRVTEVDGG
                     TSRDVRVLEVQGEPGRATGAHAHTGDEHHLVLSGRWRLTQGEETFELGPGDYLTWDAS
                     IPHDVENIGAEPGRLLLIYARNMRGKE"
     gene            2133..2528
                     /locus_tag="VMZ33_03130"
     CDS             2133..2528
                     /locus_tag="VMZ33_03130"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR16257.1"
                     /translation="MEIVKKHPTAKAPPETFTGDAWLDPIVRGEAPSHIRVSVVRFAP
                     GARNAWHVHALGQTLHVLDGVGRVQARDAAALQVRAGDTIHTPPGEWHWHGAAPDQFM
                     THLAIWEAPPDGEETTWGALVTDAEYEPQ"
     gene            complement(2522..3136)
                     /gene="rdgB"
                     /locus_tag="VMZ33_03135"
     CDS             complement(2522..3136)
                     /gene="rdgB"
                     /locus_tag="VMZ33_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HUR16258.1"
                     /translation="MSRRRLLIATGSAHKLAELSEMLDLPNTDLVSLADVGLSDTAEE
                     IGHTFLENAQIKADHYAQLSGLPTLADDSGIEVDYLDGRPGVFTRRYAGPDATDADNN
                     DKLLAELSGVEPSRRTGRYRCVLVMVDSSIGLRLEADGTFEGQVALAPRGTEGFGYDP
                     IFEPSTEPSGGRTVGQLSPQEKNAISHRAIAARSMRAQLIAKGY"
     gene            complement(3184..3948)
                     /gene="rph"
                     /locus_tag="VMZ33_03140"
     CDS             complement(3184..3948)
                     /gene="rph"
                     /locus_tag="VMZ33_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HUR16259.1"
                     /translation="MSIGGGRFRADGRSPGQLRRVTFELDAQKWAEGSCIVSFGDTRV
                     LCAASVEDRVPPHLRGKGVGWVTAEYSMLPRATNERTQREVTKGRQGGRTMEIQRLIG
                     RALRGVVDTSKIGDRTVTIDCDVLQADGGTRTASITGGYVALVLALRKLGASQAVTGQ
                     IAAVSVGIIDSVPMLDLDYSEDSRAEVDFNVVGTDQRAYVEVQGTAEHKAFDRAAMDK
                     LLDLGDAGLQQLFKMQRTVLGLAAKEPEEPRPSVAT"
     gene            complement(3945..5018)
                     /gene="mazG"
                     /locus_tag="VMZ33_03145"
     CDS             complement(3945..5018)
                     /gene="mazG"
                     /locus_tag="VMZ33_03145"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HUR16260.1"
                     /translation="MVARPFDAGIGTLILPAADVTEAQALPGRAAHGASPADIVRGLY
                     PLTHVIRGLSGAPDTTVGEVTDAALSAGPHWISPLESLDNVASPHALPWLVARLRAPD
                     GCPWDREQDHLSLRKFLIEEAYEVHDALERGSTPDLAEELGDLLLQIVLHAHYSAEAG
                     VFDLTDVYRNVVTKIVRRHPHVFGDVVADSAAEVVRNWEQIKAEERLDRGESSAAGKD
                     GVPRAFAGLSRSLPALAYAQEMQERAASLGYDWPSVEGVLDKVAEEARELVAADSDAH
                     RREEFGDLLMVLVNLARRSGIDAESALRASSAKFSTRFAHVERQAAERVVELRNLTFD
                     ELDELWDNAKEATAPAAAVGGSK"
BASE COUNT          876 a         1755 c         1742 g          910 t
ORIGIN      
        1 cgctggtatc ggctgcgaga ccaggaaagc ggctggcgga agcgcgcggc ggtcaacggg
       61 ctaggcgccg ctacaacggc ggtcgttgcg gttgaggtcg ccgtcacgaa gttcttccac
      121 ggtgcgtggg ttgtcctgat cctgataccg ctcctgatcg cgatgatgct cttcatcaag
      181 cgtcaatacg cgtcgtcggc agctcagctc gccgtcaaac ccggggtgaa gctgcccggt
      241 ccgcaccgcg acgaacgcgc catcgtgccc gtgtctggac tcaaccgagc cgtcgtccag
      301 gcagtcaatg tcggccgatc catcgccgat gacgtgctgg cagtcctcgt gtcggacaac
      361 cccgaggatg ccgcccgtct gcgcgagcgc tgggagaaac aacttcccga tgttccccta
      421 ataatcgtgg agtcgcccta ccgaaccatc gtgggtcccc tgcttgcgta cctggacgtc
      481 ctcgatcaga tgtggccggc gggcaaggaa cagccgatca cgttcgtggt gatacctgag
      541 ttcgtggccc ggcattggtg ggagcgcctg ctctacaacc agtcggcggc acgactcaga
      601 cgagctttgc tcgggcgcgc ccacaccgtc gtcgtgaatg tgccgtatcg acgggaggag
      661 actgccagct cagtctccaa cgtgagcgag cctccgggct agcgactccg aaagaggcgg
      721 cgggcaatct gcccaccgcc tcggaagcct ggagtgccgg gactcaggcg gccttcgcga
      781 gcactggcgt tgccgttgat gattccggcg cgccgacgag cacgaagctc gactgccagt
      841 agccgttgga cacgatctgc agcctgatgt tgagcaatgc cattgactcg agctcgcgcg
      901 ccgcggccag aggacccacg tgaacggggc ggaagccgag tgacatcgca agggttgcga
      961 aggtttcgcg ggctgtgtcg tcgtctatgg caaagagcgc gtcaagggtc gtgccatgcg
     1021 tctgagggtt ggcctgcacg ctgccgaaga gcgtgttgaa gcccttgacc agcttggcgt
     1081 cgccgaggat gccggcaagc tcctcagcga tggacgtccc ctccgtgaac agacccgaca
     1141 tgtcgccctt gagcgggttc gttacgtcga tcacgacctc gtgcgcgatt accggcgcga
     1201 tctcacgggc cacatcgaag gcggccgttg ctggaaccgc gaagatcacg acgtctgccg
     1261 ccgccactgc gtctgcgagc gactcggccg cagtggcgtt gatctctgac gcagtcgccg
     1321 cggcgctgtc gttgttgcgc gaagcgatga caacctggtg tccagcgcga accgcgctga
     1381 caccgagtgc cttggcgatg ttgccgctgc cgatgatgga aatgttcacg aagtcctccg
     1441 tgccgcgcga ctcgccgcgg ctttggtatt gtttgctgta gcagaacttt gcttgctaca
     1501 ggcatagtct gacacatact ttgccggagg gcaagtagtt tgaccgccaa taaacctgac
     1561 aggcttcctg tgggtgcgga tcggccgcga cccccgattg ggcgagaggt acgccattgg
     1621 cgaagggcgc gagggctgac gctcggccaa gttggcgaaa ggagccagct caacatcggg
     1681 tatctgtcac agatcgagaa cgagaaggcg ctcccgtccc tggatgcgct gatggctatt
     1741 gccggcgcgc tggatgtgcc ggcggcgtgg ctgatgatcg actcgtcgca gccaccacgc
     1801 gttgtcaggg ccacggaacg gccgacagtt gccacatccg acgccggcgg tcgtgtgact
     1861 gaggttgatg ggggcacgtc gcgtgacgtc cgcgtgctcg aggtccaggg cgagcctgga
     1921 cgtgcaaccg gcgcccacgc gcacactggc gacgagcacc atctcgtgtt gtcgggccgc
     1981 tggcgattga cccagggtga ggagactttc gagctcggcc ccggcgacta cctgacctgg
     2041 gacgcgtcca ttcctcacga cgtcgagaac atcggtgcgg agccgggtcg gctcctgctc
     2101 atttacgccc gaaacatgcg aggtaaggag tgatggagat tgtcaagaag cacccgacgg
     2161 caaaggcacc gccggaaacg ttcaccggcg acgcgtggct ggacccgatc gtccgcgggg
     2221 aggcaccgtc ccacatccgc gtgagcgttg tccgtttcgc gcccggcgcg cgaaacgcgt
     2281 ggcacgtcca cgccctgggc cagacgctgc acgtcctcga tggcgtgggc cgcgtccagg
     2341 cccgcgacgc ggctgccctt caggtgcgcg ccggggacac catccacacg ccacccggcg
     2401 agtggcactg gcacggggct gcacctgacc agttcatgac ccatctggcg atctgggagg
     2461 cacctcccga cggcgaggag acgacctggg gcgcactcgt gaccgacgcc gagtacgagc
     2521 ctcagtagcc cttggcgatc agctgcgcgc gcatcgatcg tgcggcgatg gcgcgatgtg
     2581 agatcgcgtt cttctcctgc ggtgatagct gaccgacggt ccgtccgcca gacggctcgg
     2641 tcgacggctc gaatatcggg tcgtacccga agccctcagt cccgcgtgga gcaagggcga
     2701 cctggccctc gaacgtgccg tcggcctcaa gtcgcagccc gatcgaagaa tcgaccatca
     2761 ccaggacgca ccggtagcgc cccgttcgcc tcgacggctc gacgccggac agctccgcca
     2821 gtagcttgtc attgttgtcc gcgtccgtcg cgtcaggccc tgcgtagcgc cgcgtaaaga
     2881 ctccgggtcg gccgtccagg taatcgacct cgatccccga gtcgtccgcc aacgtcggga
     2941 gccctgaaag ctgcgcgtag tggtcggcct tgatctgcgc gttctccagg aacgtgtgac
     3001 caatctcctc cgcggtatcg gacagcccga cgtcggccag cgagaccagg tcggtgttcg
     3061 gcaggtcgag catctcgctc agctcggcga gcttgtgggc cgagcccgtg gcgatgagta
     3121 agcgtctccg cgacaacttc tatccccgca cacggcccgc gggcagctgc tgcgcagtca
     3181 ccgtcacgtc gccacgctcg ggcggggctc ttccggctcc tttgcggcga gcccgagaac
     3241 cgtgcgctgc atcttgaaca gctgctgcag gccggcatcc cccaggtcga gcagcttgtc
     3301 catggccgcg cgatcgaacg ccttgtgctc ggcggtgccc tgcacctcca cgtaagcgcg
     3361 ctggtctgtg ccgacgacgt tgaagtcgac ctccgcgcgg gagtcttccg agtagtcgag
     3421 gtcgagcatg ggcacgctat cgattattcc gacgctcacg gctgcgatct ggcccgtcac
     3481 ggcctgggag gcgcccagct tgcggagggc gagcaccagg gcaacgtagc cgccggttat
     3541 ggacgcggta cgcgtgccgc catcagcctg cagtacgtcg cagtcgatgg tcacggtccg
     3601 gtcaccgatc ttggacgtgt cgaccacgcc gcgcaaggca cggccgatca gccgctggat
     3661 ctccatggtt cggccaccct ggcggccctt ggtcacctcg cgctgggttc gttcgttcgt
     3721 ggcccgcggg agcatcgagt actcggccgt gacccagccc acaccctttc cgcgcagatg
     3781 cggcggaacg cgatcttcga cggacgcggc gcacagaacg cgggtgtccc cgaaggaaac
     3841 gatgcacgaa ccctcggccc acttctgggc gtcgagctcg aacgtcacgc gacgcagctg
     3901 gccgggcgac cgaccgtcgg cgcgaaagcg cccgccaccg atgctcactt gcttcctccc
     3961 accgccgcgg caggagccgt ggcctccttg gcgttgtccc agagctcatc gagctcgtcg
     4021 aatgtcaggt tgcgcagctc cacgacacgc tcggcggcct gccgttcgac gtgtgcgaag
     4081 cgcgttgaga acttggcgct cgaggcacga agcgcggatt cggcgtcgat accgcttctg
     4141 cgcgccaggt tgacgaggac catgagcagg tcgccaaatt cctcgcgacg atgcgcatca
     4201 ctatccgcgg ccaccagctc acgcgcttcc tcggcgacct tgtcgagcac gccctcgacc
     4261 gacggccagt catagcccag ggaagcggcc cgctcctgca tctcctgggc gtaggcgagt
     4321 gccggtagcg acctcgacag accggcgaac gcccgcggga cgccgtcttt gcccgcggcc
     4381 gacgactcac cgcgatccag acgttcctca gccttgatct gctcccagtt gcgaacgacc
     4441 tctgccgccg agtctgcaac gacatcgccg aagacgtgcg gatgccggcg gacgatcttg
     4501 gtcacgacgt ttcgatagac gtcggtcagg tcgaacacgc cagcctcagc cgaatagtgg
     4561 gcatgaagca cgatctgcag caacaggtcg cccagctcct ccgccaggtc gggggttgat
     4621 ccccgttcga gcgcgtcgtg gacctcgtac gcctcctcga tcaggaactt gcgcaggctc
     4681 aggtgatcct gctcccggtc ccacgggcag ccatcgggcg cgcgcagccg cgcaaccagc
     4741 cacggcagcg catgcggact ggcgacgtta tcgagcgatt ccagtggcga aatccaatgc
     4801 gggcccgcag agagcgccgc gtccgtgacc tcgcccacgg tcgtgtcagg tgccccggag
     4861 aggccgcgaa tgacgtgcgt gagcggatag aggccacgca cgatgtctgc cgggctcgcg
     4921 ccgtgggcgg cccgtccggg cagagcctgt gcttcggtca cgtcagcggc aggcaggatc
     4981 agcgtcccga tgccggcgtc aaacggacgt gcgaccacgc cagcaggcga cagtatctgt
     5041 agacctgcgt cgtgatcgag gccaaaccga gcgtgcagct cggacagcac gtcgttcagc
     5101 tgcgtgggcg cgaggtcagc cgtcgccgcc ggcctctagc cccgggtcct ctgcggcgga
     5161 ctcgtcggcg cgagtgatcg tgccggcgtc ttcggcagcc gtcttcttgg gttcgtacca
     5221 gtcggcgaag gcgttggcct cgatgtcggg aacttggtcc gggtcgaatg cccgctgttc
     5281 ctt
//
LOCUS       DATLCO010000127         5782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3060876, whole genome shotgun sequence.
ACCESSION   DATLCO010000127 DATLCO010000000
VERSION     DATLCO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060876"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VMZ33_03150"
     CDS             complement(<1..224)
                     /locus_tag="VMZ33_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16261.1"
                     /translation="MQRLMIPMVLFGAALLVRAAVGGIFGGPAYPDSYYYVHVANQLA
                     AGDGFTSQYIWNFVDVGGSLPLDPTLPIAS"
     gene            338..1255
                     /locus_tag="VMZ33_03155"
     CDS             338..1255
                     /locus_tag="VMZ33_03155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HUR16262.1"
                     /translation="MSTSDRASARRAWLRRTVSHAAVLAGLIFCAYLVFVAAPVKGSL
                     GFDVAAYWSVDLAAPYHGSVGDHGFFAYSPAIALLLAPLTQLPWPVFLGLWYALLVGA
                     LVFLGRRSTLLLLAFPPVAIDLYHGNIHLLLACAVVIGFRYPQAWALVLLTKVTPGIG
                     LLWFAVRREWRQLGLALGATAAIVAITAVLLPTQWLSWFAMLAASAGVEPPWPALPVP
                     IWLRLPIAAAIVVWGARRDARWTVAVAAAISVPALWPGAFAILAAVWPLRRSQEAHSG
                     QEAATARPEDRGYHAADAGQRGRPEPVSA"
     gene            1215..2039
                     /locus_tag="VMZ33_03160"
     CDS             1215..2039
                     /locus_tag="VMZ33_03160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUR16263.1"
                     /translation="MPDSEVDPNPSRPSVSLVLPAYNESARIGSALDELFGYLNRLGP
                     ARDGGRPATDMGAVEVIVVDDGSDDDTAAIVERRPEAHATQRPNLRLLREPHTGKGGA
                     VKAGILVADSDYILFADADLATPPDQLPLLTDALARGDLALGSRVHPDGTDRRRTQPL
                     HRRALGKVFHGLAALWVTGPVPDTQCGFKGFRRGAARDLLGRQKIGSIVFDAEVIHLA
                     RRRGYSMAIVPVQWSDKRGSRMRVGAGLALRVLFDLARIPVIHRGVGRHQEASDPA"
     gene            2062..3231
                     /locus_tag="VMZ33_03165"
     CDS             2062..3231
                     /locus_tag="VMZ33_03165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR16264.1"
                     /translation="MPFPSVTQSRSAPFVARRVAFDDIRRADWDRLLAATASPTPFSR
                     WTFHRAWWDAYGATAHEEYLLLADRAAGETRAIVPLMHRHEVEADDASTHTVVREHAP
                     RCAPVDGQAMAIMFGASYHADYATALAAPEDLGAAAVTLAHALADESQLFDGDALWDV
                     VDLRRLRGMDPSLPALDAAMRAVGEDHGWRTNCEEEDVCPVVTVRGDTWDEYLATLEK
                     SDRHEIRRKMRRAATVGSLAIEIVPPSPEVIEDFISLHDQRFGEDGLFPDNEGGRRSR
                     LFVQRLAELEQAEGPSCQLHVARVTCGAALIFTLLAFDDGQTCFMYNGGMDPEARQAS
                     PGVTGVGMYFQDRIAAGRRRFDFLRGNERYKYEWGAVDEPIFRLLVQRGSAATGS"
     gene            3231..4457
                     /locus_tag="VMZ33_03170"
     CDS             3231..4457
                     /locus_tag="VMZ33_03170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUR16265.1"
                     /translation="MDSDRIGNDRIVVTQLMASGAGGGAQVHVQSLVEHLDPVRFDVN
                     VISLSDGPAVRRIRAAGYCVHVIDVTDDDLAVEAVLALLRVRPPDILHNHMYRAEVVG
                     TRAALALADQGLARPYVVGTVHSSRVRSEEDKARLRWLTPSMDRLVAVSRAMVTKLEA
                     EGRTSAPIELIYNGVDLKRYEYTEACCTLPEEYGFPEGSPLVGVVARLEPEKGHATLI
                     EAWPRVLEQVPEARLLIVGEGSQRQTLEARADALNLLGCGCSAPDHSVGTRHARPEAK
                     VLFTGLRDDVPAVTAALDVAVLPSYREAQGLAILEALALRRPVVATRVGGVPEMIEHR
                     RTGLLVPPRDPAALADAIATVLTDHPLADMIAKAGHDFVIANFSLDHMLAAVSAIYEE
                     GAAAISLRGTTSRTAA"
     gene            4661..5323
                     /locus_tag="VMZ33_03175"
     CDS             4661..5323
                     /locus_tag="VMZ33_03175"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HUR16266.1"
                     /translation="MSNDYRTSGTISVSGIGCVAIEPDVADVLLGVNISRKTVAEARA
                     EAATTMAAILATVAEAGVPKRDVQSSFLSVQPRYDYRDGRPPELSGYEMNNVATITVR
                     DLAKLGDVIDGSLRAGATSMDGLEFRLADPAPAEREARIRAMANARSRADVIAEAAAL
                     TITGVESVTEGGAMPPRPFAAKAERMMMAQDAATPVETGTMEIEVTVAVTYRTTAWAN
                     RS"
     gene            complement(5320..>5782)
                     /locus_tag="VMZ33_03180"
     CDS             complement(5320..>5782)
                     /locus_tag="VMZ33_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16267.1"
                     /translation="DLIRSEILRLEPQMVYFPLGVGGHVDHQLCREVGLSLLAERRAW
                     VMPGPSLVGRTTFYEDFPYSWWTDYDGPNRPNGPVLDLPAGVSVEPQYADISDVIDRK
                     AAGIQIYASQLDRLFESPQALLDDLYGFHARTALAGGVNGLAERYWATVTP"
BASE COUNT          913 a         1963 c         1905 g         1001 t
ORIGIN      
        1 ttggacgcga tggggagtgt cgggtcgagg ggcaggcttc cgccgacgtc cacgaagttc
       61 cagatgtact gggacgtgaa gccgtcgcca gccgccagct ggttggctac gtggacgtag
      121 taataggagt cggggtacgc cggacctccg aatatgcctc caaccgccgc tcgtaccagt
      181 aaggcggctc caaacaggac catcgggatc atgagccgtt gcacgttagc aacgcccgtc
      241 ctaatggcga ccccgtacga atgtgccagg gcgactggct ccgcggtacg aatcggccat
      301 cggaattcgg cggcttcatg attaccgtcg gtagcaaatg tcgacctcgg atcgagcttc
      361 cgcgcgccgc gcctggcttc gtcgcacggt gtctcatgcg gctgtcctcg caggcctgat
      421 cttctgcgcg tacctcgtgt tcgtagctgc gccggtcaaa ggctcgctcg gcttcgacgt
      481 cgcggcctat tggagcgtgg acctggctgc gccgtaccac ggcagcgtcg gggatcacgg
      541 gttctttgcc tactcgccgg cgatcgccct tctcctcgct ccgctgacgc agttgccctg
      601 gcctgtcttc ctcggcctct ggtacgcgct gctggtcggc gcattggtgt tcctgggccg
      661 tcgctcaacg ctgcttctat tggcatttcc gccggtggcg atcgacctct accacggcaa
      721 catacatctg ctgctcgctt gtgccgtcgt catcggcttc cgctacccac aagcgtgggc
      781 gctcgtcctg ttgacgaagg tcactccggg gatcggcctc ctctggttcg ctgtgcgtcg
      841 cgagtggcgt cagctcggac tcgcgctcgg cgcgactgcg gcgatcgtgg cgatcaccgc
      901 cgtcctctta ccgactcagt ggctgtcctg gttcgcgatg ctcgccgcga gcgccggtgt
      961 cgaaccaccg tggcccgccc tgccagttcc gatctggtta aggctcccaa tcgctgccgc
     1021 gatcgtcgtc tggggcgctc gacgcgacgc tcgttggacc gtggccgtgg ccgcagccat
     1081 ctccgtgccc gcgctgtggc ccggtgcctt cgcgatcctg gccgcggtat ggccacttcg
     1141 acgcagtcag gaggcgcact cgggacagga ggcggcaacg gcccgacccg aggatcgtgg
     1201 ctaccatgcg gcggatgccg gacagcgagg tcgacccgaa cccgtctcgg cctagcgtct
     1261 ccctggtgct gccggcatac aacgagtcgg cacgaatcgg ctccgctctc gatgagctgt
     1321 ttgggtacct gaaccgcttg ggtcccgcgc gcgatggcgg ccgtccagct acggatatgg
     1381 gggccgtcga ggtcatcgtc gtcgacgacg gcagcgacga cgacactgcg gcgattgtgg
     1441 agcggcgacc cgaggcccac gccacacaac ggccgaacct gcgcctgctc cgggagcccc
     1501 acaccggaaa gggcggggcc gtcaaggccg gcatccttgt cgccgactcc gactacatcc
     1561 tcttcgccga cgccgacctg gccacaccac ccgaccagct gccgctcctc accgatgcac
     1621 tggccagggg cgatcttgcc cttggctcca gagtccaccc cgacggaaca gaccgccgcc
     1681 gaacgcagcc gctccaccga cgggcgctgg gtaaggtctt ccacggtctg gcggcgctat
     1741 gggtcaccgg cccagtgcct gacacgcagt gcgggttcaa gggctttcgt cgcggcgcgg
     1801 cacgcgacct gttgggacgc cagaagatcg gcagcatcgt gttcgacgcc gaggtcatcc
     1861 acctggctcg tcgtcggggc tacagcatgg ccatcgtgcc cgtccagtgg tcggacaaac
     1921 gcgggtcgag gatgcgcgtc ggggccgggc ttgcgctgcg tgtgctgttc gaccttgccc
     1981 ggattccggt catccatcgc ggcgtggggc gccaccagga ggcgtccgac cccgcttagc
     2041 ggtaaggtga actaaccagt agtgcccttt ccctcggtta cccagtccag gtccgcgccc
     2101 ttcgtcgctc gcagagttgc cttcgacgac attcggcgcg ccgattggga tcgcctcctt
     2161 gcagccacgg cctcgcccac gcccttctca cgatggacgt ttcaccgcgc ctggtgggac
     2221 gcgtacgggg ccacggccca cgaggagtac ctgctcctgg ccgaccgcgc cgccggagag
     2281 acgcgcgcaa tcgtgccgct catgcaccgc catgaggttg aggctgacga cgcgtcgact
     2341 cacaccgtcg tgcgcgagca cgcgccgcgc tgcgcaccgg tggatggcca ggcgatggcg
     2401 atcatgttcg gagcttcgta ccacgccgac tacgcaacgg ccctggccgc acctgaggat
     2461 ttgggcgcag ccgcggtgac actcgcccat gctctcgctg acgaatcgca gctgtttgac
     2521 ggtgacgcgc tgtgggacgt cgtggacctg cgccggctcc gcgggatgga cccttcgctt
     2581 cctgctctgg acgcagcgat gcgggccgtc ggcgaggatc acggctggcg caccaactgt
     2641 gaagaagagg acgtgtgccc agtggtcacg gtccgcggcg acacctggga cgagtacctg
     2701 gccacgctgg aaaagtcgga tcgccacgag atccgacgca agatgcgccg cgccgcgacc
     2761 gtcggaagtc tggcgataga gatcgtgccg ccgagccccg aggtcatcga ggacttcatc
     2821 agcttgcacg accagcgctt cggtgaggac gggctgttcc ccgataacga gggtggccgc
     2881 cgcagccgac tgttcgtcca acgattggcc gagctggagc aggcggaggg gccgtcttgc
     2941 cagttgcacg tcgcccgggt gacctgcggc gcggcgctga tcttcacgct cctcgcgttc
     3001 gacgacggcc agacgtgctt catgtacaac ggcggcatgg atcccgaggc gcggcaggca
     3061 tcaccgggcg tcaccggcgt aggcatgtac ttccaggacc gcatcgccgc cggccggcga
     3121 cgattcgact tcctgcgcgg caacgagcgc tacaagtacg agtggggcgc cgtcgacgag
     3181 cccatcttcc gactcctcgt tcagcgcggc tcggccgcga cggggtcctg atggatagcg
     3241 accgcatcgg taacgaccgc atcgttgtca cccagctcat ggccagcggc gccggtgggg
     3301 gtgcgcaggt tcacgtgcag agcctcgtgg agcatctcga tcccgtccgc ttcgacgtta
     3361 atgtgatctc gctgtccgac ggcccggcgg tgcgccgcat ccgcgccgcc ggctactgcg
     3421 ttcacgtcat cgacgtgacg gacgacgacc tcgccgtcga ggccgtgctg gcgctgctgc
     3481 gcgtgcggcc gccggacatc ctgcacaacc acatgtaccg ggccgaggtc gttggaaccc
     3541 gcgctgcgct cgctctcgca gatcaagggc tggccaggcc gtatgtcgtt ggcacagtcc
     3601 actcgagccg cgttcggagc gaggaggaca aggcgcgtct gcgctggttg acgccgtcga
     3661 tggatcgcct ggtcgctgtc agcagggcga tggttaccaa gctggaggcg gagggtcgga
     3721 catccgcgcc catcgagctc atctacaacg gcgtcgatct caagcgctac gagtacaccg
     3781 aggcgtgctg cacgctgccg gaggagtacg gcttccccga gggctcgccg ctcgtcggcg
     3841 tcgtggccag gttggaacct gagaaggggc acgccacgct gatcgaggcc tggccgcgcg
     3901 tcctggagca ggttcccgaa gcgcggctgt tgatcgtggg cgaggggtcg caacgccaga
     3961 cgttggaggc acgggccgac gcgttgaacc ttctgggctg cggttgctcc gcgcccgatc
     4021 actcggttgg tactcgtcac gcacggcccg aggctaaggt gcttttcacg ggccttcgcg
     4081 acgacgtgcc cgccgtcact gccgccctgg acgtggccgt gctgccctcc taccgcgagg
     4141 cccagggcct ggcaatcctt gaggcgctgg ccctacgccg cccggtggtc gcgactcgtg
     4201 tcgggggagt gccagagatg atcgaacatc gccgaaccgg cttgctcgtc ccaccgcgcg
     4261 acccagcggc actggccgac gccatcgcca ccgttctgac cgatcatccc ctggcagaca
     4321 tgattgccaa ggctggccac gacttcgtga tcgcgaactt ctccctggac cacatgcttg
     4381 ccgcggtatc agccatttac gaagaggggg ccgccgccat ttccttacgc ggaaccacat
     4441 cgagaacggc cgcctagctg acgctgcacg tgggacttac gctcgccgca cagcacgtca
     4501 tgccggttga gctgcgtcgg ccctgcgcgt gtccctacgg tcgcttgtca gcggacgttg
     4561 gcacattcga gccccgctgc catgtccttc gagtatctcg cgtacgaaac tttcgccact
     4621 cgctttctgg caatgaagac acgatccaaa cggggatgga atgagcaacg actacagaac
     4681 cagcgggacg atctcggtat ccggtatcgg ctgcgttgcc atcgagcctg acgtcgctga
     4741 cgtgctactc ggcgtcaaca tctcgcggaa gaccgtcgct gaggcacgcg ccgaggcggc
     4801 tacgaccatg gccgcgatct tggccaccgt tgcggaagcc ggcgtgccga agcgtgacgt
     4861 ccagagctcg ttcctctccg tccagccgcg ctatgactat cgcgacggcc ggccgcccga
     4921 gctgtccggc tatgagatga acaacgtcgc cacgatcacc gtgcgcgacc tggccaagct
     4981 cggcgacgtg attgacggtt cgctgcgtgc cggtgccacc agcatggatg gcctcgagtt
     5041 ccgtttggcc gacccagctc cggcagagcg cgaggcccgt atccgcgcaa tggccaacgc
     5101 ccgcagccgc gccgacgtga tcgccgaggc cgcggctctg accatcaccg gcgtcgagtc
     5161 cgtgaccgag ggtggcgcga tgccgccgcg accattcgcg gccaaggctg agcggatgat
     5221 gatggcccag gacgcggcaa cgccagtgga aaccggcacc atggaaatcg aggtcaccgt
     5281 cgccgtcacc taccggacga cggcctgggc gaaccgctcc tagggcgtga ctgtcgccca
     5341 gtagcgctcg gcgaggccat tcacgccgcc agcgagcgcc gtccgcgcat gaaagccgta
     5401 caggtcatcg agcagcgcct gcggtgactc gaatagccgg tcgagctggc tggcgtagat
     5461 ctggatgccg gcggcctttc gatcgatcac gtccgagata tcggcatact gcggttcaac
     5521 gctgacgccc gcaggcaggt cgagcacagg cccgttcggc cggttgggcc cgtcgtagtc
     5581 ggtccaccac gaatagggga agtcctcata gaacgtcgtc cggccgacga gcgacggtcc
     5641 gggcatgacc cacgcgcgac gttcggcgag cagcgacagg ccgacctccc ggcatagctg
     5701 gtggtcgaca tggccaccga caccaagcgg gaagtagacc atctgtggct cgagccgcag
     5761 gatctcagaa cggatgaggt cg
//
LOCUS       DATLCO010000128         5374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2392373, whole genome shotgun sequence.
ACCESSION   DATLCO010000128 DATLCO010000000
VERSION     DATLCO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392373"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VMZ33_03185"
     CDS             complement(<1..777)
                     /locus_tag="VMZ33_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HUR16268.1"
                     /translation="MPERTFREEGASVTPGALIPWMPSSEIRQRYLDFFAARDHEVVP
                     SASLVPGGDQTLLFTNSGMVQFKEVLTGQETRSYKRAVDCQRCLRVAGKHNDFEEVGR
                     TTRHHTFFEMLGNWSFGDYFKREAIVWAKELLTEVYKIPWERLGATTYKDDEEARQYW
                     REVGMPEERMAIWGDVDIGDDRNFWRMADTGPCGPCSEIHFDRGAHLSEGPHCIPDHD
                     ENCPRWLEIWNLVFMEFDQKPDGPRVPLPFKSVDTGMGFER"
     gene            819..2627
                     /locus_tag="VMZ33_03190"
     CDS             819..2627
                     /locus_tag="VMZ33_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUR16269.1"
                     /translation="MSVDAVRVTCPRCETEVSTDARFCMSCGMSLGDPSADDAAREQK
                     VVASAPSPLVDKMRAAKLTGERKPVTALFADVVGSTTLAESMDPEDWTQIVNEAFDLM
                     SKAVFHYEGTIAQLQGDAMLAFFGAPVAHEDDPDRAIRAALEMIEQIDEYARQLQRTH
                     GIEFRIRAGINTGPVIVGNVGSDLRYEYTALGDAVNVAARVQTSAEPRTVLVTANTMR
                     FAADAFDFEDLGDIELKGKTEPVQLYRVIGIKAQPGRRRGLESVGLSSPLVGRSAELE
                     SLRALFEVAVAGRGRVAVLIGEPGIGKSRLLAELRALIPTATWVEGKCVTYGRTVPYH
                     LVLDMVRSMLGISLSNSEAEERQIMTTRLRELLGDEASDVGPYIAHLLSLPLSQVERD
                     MVQVDPEMLQSRYVAAIFRVIRGHLAAGPLVLVCEDIHWADPASVEMVIQLMALMTTQ
                     PVLLILAGRNETDSPGWRLVTQVRTTMGDALTEIRLQPLSDDASRELVSNLLEIESLP
                     EQVRDKILERAEGNPFFVEEVIRMLIERGAIVRSTNRWVADESITAVEIPETLHSLLL
                     ARIDQLPDDAKRSLRVASVIGRQFPVRVLERVLATE"
     gene            2640..4163
                     /locus_tag="VMZ33_03195"
     CDS             2640..4163
                     /locus_tag="VMZ33_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16270.1"
                     /translation="MTAQNLQLDTLEAKGLIRLASFQPELEYLFRHALVQDTAYDSLL
                     KQERRALHRSVGDAIEELYPERHGDLAAVLAMHFEQAGDTTRAISYLMAAADFAYARN
                     AVVEAFDLYGRAAAMLPPRSEDEAEATLRRRVEIGIGRARTGFAFQERSEQIAGIKEA
                     VADAERLGDLRLIGDVHLYNALHRQFEGERTETSEPLRKSLERVTEIAQALNDPPFGA
                     IPKSIIGITQVFSGELSEGIANLEEAAPLLAQKRDFVGSSFALMALGVGYARLGEFEK
                     ADAAVQHAREVAAGGDIIARIDTMIGESMVASMKGDFDAAALLAQQCSSMAEDAGATA
                     CVVGSNVVLGESLMRQGQFAGAKIAFDRSHEVAEVTNQRMFRPTVAAFRRSIAASLGD
                     FNLAGRSFEDALQDAREMHDRFAEAGVYWLRAGAANPTAEADEAQTLTDFATAEGLFA
                     QMGVRPYQARVLREWGMTLRKLGHRSDGDEKLRRAAGLFTDMGLAREADELTAELSA"
     gene            complement(4182..4676)
                     /locus_tag="VMZ33_03200"
     CDS             complement(4182..4676)
                     /locus_tag="VMZ33_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16271.1"
                     /translation="MTAEQVNLYFGIALIVAVGLIALAALIAAMALLRVGRDIRHTAR
                     AAEKVLASVDQELPAALRDLRATSAQLARLAEEVPPRLERVDGLLDEATASVQSLRAT
                     IETAEDIVRGPQAALDRASRTVRDVGVGLARGADRLMRGVQQRRGGGSSGGAEGGAGE
                     GRAD"
     gene            complement(4673..4969)
                     /locus_tag="VMZ33_03205"
     CDS             complement(4673..4969)
                     /locus_tag="VMZ33_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16272.1"
                     /translation="MPEIGPALILAAILGVFHTGLYLVIRGSGGLRLPLVAVAAILGA
                     FAGQQLGARLGDPLMIGDFGLIWASVLSWLGIVLVAAAGMLSPSRGPRSTGGAR"
     gene            complement(4962..>5374)
                     /locus_tag="VMZ33_03210"
     CDS             complement(4962..>5374)
                     /locus_tag="VMZ33_03210"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HUR16273.1"
                     /translation="IDVDGTRVGDHRGAAAYTIGQRQGLGVALGEPRFVNTIDAATNI
                     VTLGRREDLYRQDFEVESVTFVDGEPPDGGDDFSADVRIRHRAEPLPATVTRGATDRT
                     WSVHLERPAWAPAPGQAAVFYRGDEVIGGGRIDA"
BASE COUNT          928 a         1763 c         1793 g          890 t
ORIGIN      
        1 gcgctcgaag cccatgcccg tgtcgacgct cttgaagggc agcgggacac ggggaccgtc
       61 cggcttctgg tcgaactcca tgaagacgag gttccagatc tcgagccagc gcgggcagtt
      121 ctcatcgtgg tcggggatgc aatgcggacc ctcggagagg tgggcaccgc ggtcgaagtg
      181 gatctcgctg cacgggccgc acgggccggt gtccgccatc cgccagaagt tgcggtcgtc
      241 gccgatatcg acgtcgcccc agatcgccat gcgctcctca ggcatgccga cctcgcgcca
      301 gtactggcga gcctcctcgt cgtccttgta cgtcgtcgca ccgagacgct cccacgggat
      361 cttgtagacc tccgtcagga gctccttggc ccagacgatg gcctcgcgct tgaagtagtc
      421 gccaaagctc cagttgccga gcatctcgaa gaacgtgtgg tggcgcgtcg tccgcccgac
      481 ctcctcgaag tcgttgtgct tcccggccac gcgcaggcag cgctggcagt caaccgcgcg
      541 cttgtacgag cgcgtctcct gacctgtcaa cacttccttg aactggacca tgcccgagtt
      601 cgtgaagagc agcgtctgat cgccgccggg cacgagcgac gcgctgggca cgacctcatg
      661 gtcacgcgcg gcgaaaaagt cgaggtagcg ctgtcggatc tccgaggacg gcatccatgg
      721 gattagcgcg cccggtgtga ccgacgcgcc ctcctcacgg aaggtgcgtt ccggcatggg
      781 ttaatcgtag cgagaagccc cgcgcgtata gtgcgggcat gtccgtggat gctgtgcggg
      841 tgacatgtcc ccgttgcgag actgaggttt cgacagacgc ccgcttctgc atgagctgcg
      901 gcatgtccct cggcgatccg tcagcagatg acgcggctcg cgaacagaag gtcgttgcct
      961 cggcgccgtc gccgctcgtc gacaagatgc gcgctgccaa gctcaccggg gagcgcaagc
     1021 ccgtcacggc attgtttgcc gacgtcgtgg gctcaacgac tctcgctgag agcatggacc
     1081 ccgaggactg gacgcagatc gtcaacgagg cgtttgacct gatgtccaag gcagtctttc
     1141 actacgaggg cacgatcgcg cagctacagg gcgacgccat gctcgcgttc ttcggcgctc
     1201 ccgttgccca cgaggacgat cccgatcggg cgatccgtgc cgcgctcgag atgatcgagc
     1261 agattgacga gtacgcccgc cagctgcaga ggacgcacgg catcgagttc cgcattcggg
     1321 cggggatcaa caccgggccg gtgatcgtcg ggaatgtcgg ctccgacctt cgctacgagt
     1381 acacggccct gggcgacgcg gtgaacgtgg cggcccgcgt ccaaacatcg gctgagccac
     1441 ggactgtcct cgtcacagcc aacacgatgc gcttcgcggc ggatgccttc gacttcgagg
     1501 acctcggcga catcgagctc aagggcaaga ccgagcccgt ccagctgtac cgcgtcattg
     1561 ggatcaaggc acagcccggg cgtcgacgcg gcctcgaatc ggttgggttg tcgagtcctc
     1621 tcgtgggccg ctcagccgag ttggagtcgc tgcgcgccct cttcgaagtc gcggtggcgg
     1681 gtcgcggacg tgtagccgtc ctcatcgggg agcccggcat cggcaagtca cgactgctcg
     1741 ctgagctgcg cgccctgatt ccgactgcaa cgtgggtcga gggcaagtgc gtcacctacg
     1801 gccgaaccgt cccctaccac ctcgtgctcg acatggtgcg atcgatgctc ggcatctccc
     1861 tttcgaactc ggaggctgaa gagcgccaga tcatgacgac gcggctgcgc gagctcctcg
     1921 gcgacgaggc cagcgacgtt ggcccataca tcgcgcactt gctttcgctg ccactctcac
     1981 aggttgagcg agacatggtc caggtcgacc cagaaatgct tcaaagccgc tacgtcgcgg
     2041 cgatctttcg cgtgatccga gggcacctgg ccgcaggtcc gctggtgctg gtgtgcgagg
     2101 acatccattg ggccgatccg gcctcggttg agatggtcat ccagctcatg gcgctcatga
     2161 cgacccagcc cgtgctgctc atcctggccg ggcgaaacga gacggactct cccgggtggc
     2221 ggttggtaac ccaggtgcgg actacgatgg gcgacgcact cacggagatt cggttgcaac
     2281 cgctgtccga tgacgccagc cgcgagctcg tgtcgaacct tctcgaaatc gagtcgctgc
     2341 cggagcaggt tcgcgacaag atcctggagc gcgcggaggg caatccgttc ttcgtcgaag
     2401 aggtcatccg gatgctgatc gagcgcggcg caatcgtccg cagcaccaat cgttgggtgg
     2461 ctgacgagtc gatcaccgcc gtggagatcc ccgagacgct ccacagcctt ctactggcgc
     2521 gcatcgacca gctgccagac gacgccaagc gcagtctgcg tgtggcttca gtgatcggcc
     2581 gccagtttcc ggtccgagtg ctggaaagag ttctcgcgac ggaataggct tgcccagttg
     2641 tgaccgctca aaacctccag ctggatactc tcgaagcgaa gggcctgatc cggctggcat
     2701 cgttccaacc ggagctggag tacctgttcc gacacgcgct ggttcaggac accgcgtatg
     2761 actccctgct gaagcaggaa cggcgtgcgc tccaccggtc cgtcggcgac gccattgagg
     2821 agctctatcc cgagcgccac ggtgacctgg ccgcggtgct ggcgatgcac ttcgagcagg
     2881 cgggcgatac gacccgcgca atttcgtacc tcatggcggc cgcggacttt gcatatgcgc
     2941 gcaatgctgt agtcgaggcg ttcgatctct acggtcgcgc ggcggcaatg cttccgccgc
     3001 gaagcgagga cgaagccgag gccaccctgc gacggcgcgt ggagatcggc atcggccgag
     3061 cgcgcaccgg tttcgccttc caggagcgca gcgagcagat tgccggcatc aaggaggcgg
     3121 tggcggacgc ggagcgcctg ggcgacctgc gactcatcgg cgacgtgcac ctctacaacg
     3181 cattgcaccg tcaattcgag ggtgagcgca cggagaccag cgagccgctg cgcaagtcgc
     3241 tggaacgcgt gacggaaatc gcgcaagccc tgaacgaccc acccttcggc gcgattccca
     3301 aatccatcat cgggatcacg caggtttttt ctggtgagct gagcgaaggc attgccaacc
     3361 tggaggaagc cgcgccgctg ctggcacaga aacgcgactt cgtgggctcg tccttcgcac
     3421 tgatggccct tggcgtcggc tacgcgcgac taggggaatt cgagaaagcc gacgcagccg
     3481 tccagcacgc gcgggaagtg gcagcgggcg gtgacatcat cgcccggatc gacacgatga
     3541 ttggcgagtc gatggtggcc tcgatgaaag gcgactttga cgccgcggcc ctcctcgccc
     3601 agcaatgcag ctccatggcg gaagatgcag gtgcgactgc ctgcgttgtc gggagtaatg
     3661 tcgtgttggg cgaatcgctg atgcgccagg gtcagttcgc gggcgcaaag attgccttcg
     3721 accggagcca cgaagtcgcc gaggtgacca accagcgaat gttccggccc accgtggcgg
     3781 ccttcaggcg ctctatcgcg gcctcgctgg gcgacttcaa cctggcgggc agaagcttcg
     3841 aagacgcgct gcaggacgcg cgcgagatgc acgatagatt cgccgaggcc ggtgtctact
     3901 ggctgcgtgc cggggcggcc aatcccactg ccgaggctga cgaagcgcag acactgaccg
     3961 acttcgcgac ggccgagggg ttgttcgcgc agatgggtgt gcggccgtat caagcgcgag
     4021 tcttgcgtga gtggggcatg acgctgcgaa agctgggcca tcgcagtgac ggcgatgaga
     4081 agctgcgcag ggcggctggc ctgttcaccg acatgggcct cgcccgcgaa gcggacgaat
     4141 tgactgcaga gctgagcgcc tagccagcgc cccggccgag cctaatcggc gcggccctca
     4201 ccggctccgc cttctgcgcc tccggagctc ccgccgccgc ggcgctgctg caccccacgc
     4261 atcaatcgat cggcgccgcg cgcgaggccg acgccgacat ctctgaccgt gcggctggcg
     4321 cggtccaggg ccgcctgcgg gccgcgcacg atgtcctcgg cggtctcgat agtcgcccgc
     4381 agtgactgga cgctggctgt cgcctcatcg agcaggccgt cgacgcgctc caggcgcggt
     4441 ggcacctcct cggcgaggcg cgccagctgc gcactcgtgg cacgaagatc gcgcaacgca
     4501 gcggggagct cctgatccac ggatgcgagc actttctcgg cggcgcgggc cgtgtgacgt
     4561 atatcgcgac cgacgcgcag gagggccatt gctgcgataa gcgcggcgag ggcgatcaag
     4621 cccacggcca cgatcagcgc gatgccgaag taaaggttga cctgctcggc cgtcaccggg
     4681 ctcctcccgt gctacgcggg ccgcggctcg gcgagagcat ccccgcggct gctaccagaa
     4741 cgattccgag ccagctcagg accgacgccc agatcagtcc gaagtcgccg atcatcaatg
     4801 ggtcaccgag tcgcgcgccg agctgctgac cggcgaaagc gccgaggatc gccgccaccg
     4861 cgacgagcgg caggcgcagc cctcccgacc cgcggatcac caggtacaaa cccgtgtgaa
     4921 agacgcccag gatggccgcc agaatgagcg cagggccgat ttcaggcatc gatgcggccg
     4981 ccaccgatca cctcgtcgcc gcgatagaag acggcagcct ggccaggggc gggtgcccac
     5041 gctgggcgct cgagatggac gctccaggtt cggtccgtgg cgccgcgagt caccgtggcc
     5101 ggaagtggct cggcgcggtg ccggattcgc acatcggcgc tgaagtcgtc gccgccatcc
     5161 ggtggctccc cgtcgacgaa ggtgaccgac tcgacctcga agtcctgacg gtacaggtcc
     5221 tcgcgtcggc ccagggtgac gatgttggtg gcagcgtcga tggtgttgac gaaacgcggc
     5281 tcgcccagcg cgacgccgag cccctgccgt tgcccgatcg tgtacgcggc cgctccgcga
     5341 tggtcaccga cgcgcgtccc atcgacgtcg atca
//
LOCUS       DATLCO010000129         4418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_905382, whole genome shotgun sequence.
ACCESSION   DATLCO010000129 DATLCO010000000
VERSION     DATLCO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905382"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1982
                     /locus_tag="VMZ33_03215"
     CDS             261..1982
                     /locus_tag="VMZ33_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUR16274.1"
                     /translation="MDAGTAIWDIWFLSPLPGKQPLKLESRDGDPPAASRALRLVRLR
                     LRLALLTMAVLPMSLSMVFLKVGLGNAPQASSVTILGVMVALTMLMVGLTVWMTRQIL
                     RPAEVLDRSRLEMRRLYEVARADSLRDGLTGLGNHRAFQEELDREMEWYQRYEVPVAL
                     LLIDIDDLKLVNDSEGHAAGDEVLLQFGKLINDLGRFADRAFRVGGDEFAILMPHTDA
                     DGALEMARRLQARAAHPRANARAINFSGGVSACPLLATSRSQLYAQADAALYWCKRHG
                     RASIDVFHPIRDRAASQEATNELSAAIAKVVSQRLLRPVYQPIVELATGRVIGFEGLT
                     RPSPESGFADPGSMFTAAETVGRTVELDQACLHAVVAGARDMSPDQLLTINISPRTVE
                     APHFSADALLVILARYGIAPERVVVELTEREQVEDITRLQANLMAIQKAGIRVAADDV
                     GAGNAGLRLLSQFRFDIVKIDLTLVQEGAERDSSRAVLRSLRDLASRWGASVIAEGIE
                     TVAQLRVVRELGMTAGQGYLLARPAPTAELTRVDVGAIESGGLVLDRAAHSHEFMLPG
                     ANTQPTA"
     gene            2108..3028
                     /locus_tag="VMZ33_03220"
     CDS             2108..3028
                     /locus_tag="VMZ33_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR16275.1"
                     /translation="MDRKRPLKVGVQLPEVEREVRWAEVRQMAILAEDVGFDSLWVGD
                     HLLYKRPLDTVGPWEAWSTLAALSEVTSKVELGPLVACTSFHNPAVLAKKAATIDEIS
                     GGRLILGLGAGWNEPEYRAFGFPYEQRASRFEEAFTIIRALLRDGYVDFDGKFYQVRD
                     CTLHPRGPRADGPPLMIGSVGERVLNFTAPYMDSWNAWHDWFGNSPEGLVGLMAALDA
                     ALVTAGRDPAEVDRTVTVLVQLPGGTGRAAGGEIAKPTPPLKGTNEEIAAAIARYAEV
                     GVSHLQLVVDPITVSSIEAFGPILELLDRG"
     gene            3044..3880
                     /locus_tag="VMZ33_03225"
     CDS             3044..3880
                     /locus_tag="VMZ33_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16276.1"
                     /translation="MIVPPIVQTLPFVAWMLLVTLAFGTFLFGAVTAEAAPATRGYLR
                     FTAITAAILALLAWVVDTSLPAPAGDLVLRQAPADLDLVRRVALGGFAITSLLYAAVL
                     SDRENRWALGGLSLIAAGTALIAAAFSWAPTAVDGVPLAVQLFTLAVTTGGSLAAIGL
                     GHWYLVTPKLSERPLILQTRWLLGALIIQGLLFIVWTTLGGGVGQDAWEALSGSSALL
                     VWLRGLVTVGFPIVLVYMSLRTAQTRSMESATGLLYISLAAVMAGTIGAAALYISGGI
                     LV"
     gene            3880..>4418
                     /locus_tag="VMZ33_03230"
     CDS             3880..>4418
                     /locus_tag="VMZ33_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HUR16277.1"
                     /translation="MVIRVRLFAMLREHAGWREREIELPPDSSIEDAWSALVGLVPGV
                     AASRDSVRFARNRQYAAADERLADGDELVLIPPVAGGESVLRRCEITPDPIGDELLAE
                     LRRTVPTPADGAFVLFAGQTRETAGTPAPGEEAEAARYEGERVVGLEYETFDEMAIDV
                     LEAIAAEIETRFAVRRLAI"
BASE COUNT          659 a         1455 c         1512 g          792 t
ORIGIN      
        1 tcgtcaacga tgtcccgacg tatcggatcg accacatgcc gtacggcggg gtgaaggact
       61 ccgggatcgg acgggagggc ctgcgctggt cgatcgagga catgaccgag ctgcggctga
      121 tggttatcgc tgagcccgac aagcctgcgc ccagaccgtc cagctaggtc gggcctgccc
      181 cgcgcaggcc cgacgtggta ggcaacggcc cgtgctggta ccgacgttca ggatgggatc
      241 gcgaggctta ccaatgagcc atggacgccg ggaccgcgat ctgggatatc tggttcctgt
      301 caccgctccc ggggaaacag cctttgaaac tcgagtccag ggatggggat ccacctgccg
      361 cgagtagggc tctccgtctc gtccgtctgc gcctacgcct cgcgctgctc acgatggccg
      421 tcctacccat gtcattgtca atggttttcc taaaggtcgg ccttggcaac gcgccccagg
      481 catcctcagt cacgatcctc ggcgtgatgg tcgcattgac gatgctcatg gtcgggctga
      541 ccgtgtggat gacgcgccag atcctgcggc cggcagaggt gctcgaccgc tcacgactgg
      601 agatgcgccg gctgtacgag gttgcccgtg cggactcgct gcgtgacggc ttgaccggcc
      661 tcggcaatca ccgcgcgttt caggaggagc tcgaccgcga aatggagtgg taccagcgct
      721 atgaggtgcc cgtggcgttg ctgctgatcg acatcgatga cctgaagttg gtcaacgact
      781 cggagggcca tgccgccgga gacgaggtgc tgctccagtt tggcaagctg atcaacgacc
      841 tggggcgctt cgccgatcgt gccttccgcg ttggtggtga cgagttcgcc atcctcatgc
      901 cgcacactga cgccgatggc gcgcttgaga tggcgcgccg gctccaagct cgggctgcgc
      961 acccgcgcgc caatgcccgc gcgatcaact tctcgggcgg cgtctcagcg tgtccgttgc
     1021 tcgcgacgtc cagatcgcag ctgtacgcgc aggccgacgc cgcgctctac tggtgcaagc
     1081 gtcacggtcg cgcctcgatt gacgtattcc atccgatccg cgaccgcgcg gcctcacagg
     1141 aggccacgaa cgagttgtcg gcggccatcg ccaaggtcgt atcgcaacgg ctactgcggc
     1201 cggtctacca gccgatcgtg gagctggcga cgggccgcgt catcggcttc gagggcttga
     1261 ctcggccatc ccccgagtca ggctttgctg accccgggtc gatgttcacc gcggccgaga
     1321 ctgtcgggcg cacggtcgag ctcgaccagg cgtgcctgca tgccgtcgtc gcgggggcac
     1381 gcgatatgtc cccggaccag ctgctaacca tcaacatctc gccaagaacg gtcgaggcac
     1441 cccatttctc tgccgacgcg ctgctcgtca tcctggcgcg ctacgggatt gcgcccgagc
     1501 gcgtggtcgt cgaactcacc gagcgtgagc aggtcgagga catcacccgt cttcaggcca
     1561 acctcatggc gatccagaag gcaggtatcc gagttgcggc ggatgacgtt ggcgcgggca
     1621 acgccggact gcgcctactc agccagtttc ggtttgacat agtgaagatc gacctgaccc
     1681 tcgtccagga gggtgccgag cgcgactcgt cgcgggcggt gctccgctcg ctgcgcgacc
     1741 tggccagccg ctggggagcg tcggtcattg ccgagggcat cgagaccgtc gcgcagctgc
     1801 gcgtggtcag ggagctcggg atgacggccg gacagggtta cctccttgcc aggccggctc
     1861 cgaccgctga gctcacgcgg gtcgacgtgg gggcgatcga gtccggcgga ttggtgctcg
     1921 atcgggccgc gcatagccac gagttcatgt tgccgggcgc gaatacgcag ccgactgcgt
     1981 agcggccacc ggctcgttcg gccgggcgcg gctctctaaa ctcacaaagt gtcggatgaa
     2041 cgccgcctcg tcacggtcct cttcgccgag cgcgatcgca agcgcttccg ccgatagact
     2101 ccggctgatg gacaggaagc gcccgctgaa ggttggagtt cagctgcctg aggtggagcg
     2161 cgaggtgcgc tgggctgagg tgcgccagat ggcgatattg gctgaggatg tcggcttcga
     2221 ttcgctgtgg gttggcgacc acctcctcta caagcgcccg ctcgacaccg tcggaccgtg
     2281 ggaggcgtgg tcgaccctcg cggcgctgtc ggaggtgacg tcgaaggtcg agctcggccc
     2341 gctcgtggcc tgcacgagct tccataaccc ggcggtgctg gcaaagaagg cggcgaccat
     2401 cgacgagatc agcggcgggc ggctgatcct cggcctcggc gccggttgga acgagccgga
     2461 ataccgcgcc ttcgggtttc cctacgagca gcgggcgagc cgcttcgagg aggcgttcac
     2521 gatcatccgc gcgctgctgc gcgacgggta cgtggacttc gacggcaagt tctaccaagt
     2581 gcgcgactgc acgctgcacc cgcgcggacc gcgcgccgat gggccgcccc tcatgatcgg
     2641 ctcggtcggg gagcgggtgc tcaacttcac cgcgccgtac atggactcct ggaacgcgtg
     2701 gcacgactgg ttcggcaact cccccgaagg cctggtgggg ctgatggcgg cgctcgacgc
     2761 ggcactggtg acggccgggc gcgatccagc ggaagtggac cgaaccgtga cggtcctcgt
     2821 ccagctgccc ggggggacgg gtcgtgctgc cggcggtgag atcgccaaac cgacgccgcc
     2881 gctcaaaggg acgaacgagg agatcgccgc agcaatagcg cgttacgcgg aggtcggcgt
     2941 gtcgcacctc cagctcgtcg ttgatccgat cacggtctcc tcgatcgagg ccttcggtcc
     3001 gatcctcgag ctgctcgatc gcggctaggc ccgcgtcctt acggtgatcg ttccgccgat
     3061 cgtccagacg ctgcccttcg tcgcctggat gctcctggtg actctcgcct tcggcacctt
     3121 cctgtttggc gccgtgaccg ctgaggcggc gccggcgaca cgcggctacc ttcgcttcac
     3181 ggcgatcacc gccgcgatct tggcgcttct ggcgtgggtc gtggacacgt cactgccggc
     3241 acccgccggc gatctcgtcc tacgccaggc gccggccgac ctcgatctgg tgcgccgcgt
     3301 cgcactgggt gggttcgcga tcacgtcgct gctttacgca gccgtacttt ccgatcgtga
     3361 aaaccgatgg gcgctcggcg gcttgtcgtt gatcgccgcg ggcacagctt tgatcgcggc
     3421 ggcgttctcg tgggcgccaa cggctgttga cggcgtgccg cttgccgtcc agctgttcac
     3481 cctcgcggtg accaccggcg gctctctcgc ggcgatcgga ctcggccact ggtacctcgt
     3541 gaccccgaaa ttgtccgagc gcccgctcat tctccagacg cgctggctgc tcggcgccct
     3601 catcatccag ggtctgctgt tcatcgtctg gaccacgctg gggggaggtg tcgggcagga
     3661 cgcgtgggag gcgctgagcg gatcgtcggc actgttggtc tggctgcgcg gcctcgtcac
     3721 cgtcggattc ccgatcgttc tcgtctacat gtccctgcgc acggcccaga cacgctcgat
     3781 ggaatccgcc acgggccttc tctacatcag cctcgccgca gtcatggccg gcacgatcgg
     3841 cgccgcggcg ctctacatct ccggcggcat cctggtctga tggtcatccg agtacggctc
     3901 ttcgcgatgt tgcgtgaaca cgccggctgg cgagaacgtg agatcgagct gccgcccgac
     3961 tcttcgattg aggacgcctg gtccgcgttg gtggggctgg tccccggggt cgcggcttcc
     4021 cgggactcag tccgcttcgc gcgcaaccgt caatatgccg cggccgacga acgcctcgcg
     4081 gacggcgacg agctggtgct gatcccgcct gtggccgggg gcgaatcggt tctgcgccga
     4141 tgtgagatca ccccggaccc gatcggggat gagctgctgg cggagcttcg acgcaccgtg
     4201 ccgacccctg ccgacggtgc attcgtcctg ttcgccgggc agacacgcga gacggccggg
     4261 acgccggcgc cgggggagga ggccgaggcc gcccgctacg agggcgagcg tgtggtcgga
     4321 ctggagtacg agacgttcga cgagatggca atcgacgtgt tggaggccat cgccgccgag
     4381 atcgagacgc gcttcgcggt gcgcaggcta gcaatcat
//
LOCUS       DATLCO010000130         4785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9771084, whole genome shotgun sequence.
ACCESSION   DATLCO010000130 DATLCO010000000
VERSION     DATLCO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9771084"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..839
                     /locus_tag="VMZ33_03235"
                     /pseudo
     CDS             1..839
                     /locus_tag="VMZ33_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1319..3124
                     /locus_tag="VMZ33_03240"
     CDS             1319..3124
                     /locus_tag="VMZ33_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUR16278.1"
                     /translation="MARIGDRIASYELMAVIASGAMGTVYRAKDSRLGREVALKLLAV
                     DLSRDATFRARFERESRVTAGLRHPNIVPVFEAGEWLNQLYIAMQLVDGPSLAGIIAR
                     RRHLPTKQVVSIVGQLAGALDAAHVRGIVHRDVKPGNVLLLEGARGEGTDHAYLVDFG
                     VTRSVDAADSMTRTGVFLGTLSYIAPEQLAAGHVDGRADQYALAATAFEMLSSTPPFV
                     RDNEAALMWAHLNAPPPALSSVRPDLPAALTPVLQRAMSKDPEERYASSGTFAAALAA
                     AASATAPAPSAILTAAAAQTAPTVEAAPPMATEPRSRIAPPRQRKSQNRGWLPFLAGA
                     LALVLVALAGYSAMILLGAPGGTDRPQATRPVGAGPTPSPAVSLDVLPTDGATVAPQT
                     LGPGDTPTPRVVPTRTARPTRSNRTPTPRQSTQPPTATPTATPTATPTATPVETPRLP
                     VAAQGTWAVTYTRTGSTGSPPTDLGDHNRTYDIEPSCAAESSCRMLATTYDAGGEFVG
                     RITFRWNGSVYRYSGDARYYARAGGSNCVTEGGETIIGAYQTAEVVSIEPMTWDGDRV
                     IVFDGTKRITGTPTDVGAANGCEPYSLAYDARLTH"
     gene            complement(3132..3560)
                     /locus_tag="VMZ33_03245"
     CDS             complement(3132..3560)
                     /locus_tag="VMZ33_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16279.1"
                     /translation="MAIYRPRRSRWPLLLAVGVICFLLGGAVGAVVVGSRSADLEETA
                     TIISDEMGASAGLLEVVEIEYTEAVAPGGGEIEYEAALDNLSRSRERYDSVANGLAVL
                     DPDRARRIETGFARLQTMIAERAEAAAVGDGVASLREELL"
     gene            complement(3565..>4785)
                     /locus_tag="VMZ33_03250"
     CDS             complement(3565..>4785)
                     /locus_tag="VMZ33_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HUR16280.1"
                     /translation="LEHQTSVRSLDVRVVGVAGGSLMRVSRRLGRLRVSDVGPRSAHI
                     AGLSHASFAAPETLIDAHATLVAPRAGDADDYVVVQAADGRTFAATVTCAANALGDVA
                     HGSYASGNTEAARLAFEAVGKLVGDPWDGVARRVLALGTGKIADVVEEIVTGHRLRSP
                     LLVGIGGGAGALLPTLGAKLRLEWRMAPDAEILSSIGDALSLIRVEIERGATQATPEL
                     ISALHREAEDQAINAGAAPESIQVESERLADRAAIRVTATGTAAGLAPLADGTSAADC
                     EAAAEHALRRDVDCLAADKFALFVPVGAYRNEYSDVDFALVDQRAGIAATGRGRVLVG
                     LAADAHRQLDELLPTLVRHIGPLSVAPAVKLVRGGRLVDLTSLSKLDDILAAVANECV
                     LASDAHVMAIVTRS"
BASE COUNT          830 a         1661 c         1562 g          732 t
ORIGIN      
        1 tgttcgaggc acgcatgccc aagggcaagg ctgagatcag ccacatcgac ggcgtcgtcg
       61 aagtgcttca gaccgagtcg ggcggccgtc gcgtgaaggt cacttcgcgc gaggagttcg
      121 atacgccgct ccgtaccacg aagggccacg aggtcctggt ggctgagggc gaccatgtcg
      181 aggccaacca ggtcgtcgcg cggttgaccg cgaaggacgg cacgatcgaa gaggtcaagt
      241 cagtcgacaa gggccagatg gtgaaggcca aggacggcct catggttcga tcagaagacg
      301 tggtcgagcg cgagtacgca attccgcaca acgccaagct gctcgtcgag aatggccagc
      361 tggtgcgtgc gggtgacgcg atcaccgatg gtccgatcaa cccgcaggag tatctcgaca
      421 cccgcggccg ggatgccgtg cagcgctacc tggtcaagga agtgcagcgc gtgtaccgat
      481 cacagggcgt gacgatcaac gacaagcaca tcgagatcat cgtccgtcag atgctgcgca
      541 aggtgcgtat cgatcagccg ggcgactcgg agcagctgcc cttcgagctt gtcgacaagt
      601 tcgacttcga ggagatcaac aaccaggttc tggctgaggg cggcgagccc gctacggccg
      661 aaactgtcct cctcggggta accaaggctt cgctgaacac cgcctcgttc ctcgccgccg
      721 cttccttcca ggagacgacg cgcgtcctga ccgaggccgc catcaacggc gccaaggaca
      781 agctccttgg gctcaaggag aacgtcatca tcggcaagct catccccgct gggtcgggcg
      841 cgccgcagaa cctcgccgcc gctcgggccg ctgcccgccg cgccgccgcc gaggctctgg
      901 ccggaggcga gctccccgaa ggctttggcg acgaggagat caatatcttc ctcgccgagg
      961 ccgaggcacg gcgcgaggcg gagatggcgg cgatcacgga gttcaacccg ctcctgggcg
     1021 aggctgttgt tgccggtgac ggcgcggcac ctgacggagc caaccccttc ctggccgagg
     1081 acgaagagga agaggacgag gaactcgctc ccgaggacga ggtcgccgag actcctgcct
     1141 gacgtcaggc aatcaatcgg tcgagctgac gaaaccagca tcgaccgcca tacgtcttaa
     1201 cgtgcgagac ccgctacggc ccttctatcg gtcgcgcggg tctcgccatt tccgggagcg
     1261 atgcgttagc gtcgcaggag cacgtcggca gataggttgc ttgcgctagc gggtgggcat
     1321 ggccaggatc ggcgatcgga tcgccagcta cgagttgatg gcggttatcg cctcgggcgc
     1381 gatgggtacc gtctatcgag ccaaggactc aaggctcggc cgtgaggtcg cgctcaagtt
     1441 gcttgccgtg gacctgtcgc gtgacgccac ctttcgggcc cggttcgagc gcgagtcccg
     1501 agtaacggct gggctgcgcc acccaaacat cgtgcctgtc ttcgaggccg gcgagtggct
     1561 gaaccagctc tacatcgcga tgcagctcgt cgacggaccg tcgctcgccg gcatcattgc
     1621 gcggcgccgg cacctaccga caaaacaggt cgtctcgatc gttggtcagc tggccggcgc
     1681 gctcgacgcc gcccatgtgc gtggcatcgt gcaccgcgac gtgaaaccgg gcaatgtcct
     1741 gttgctggag ggggcccgcg gtgagggcac cgatcacgcc tacctcgtgg acttcggagt
     1801 aacgcggtcg gttgacgcgg ccgactcgat gacgcggacg ggcgtcttcc tgggcacgct
     1861 cagctacata gcgcccgaac agttggcggc gggtcacgtc gacgggcgag ccgaccagta
     1921 cgccctggcc gcgacagcct tcgagatgct gtcctccacc ccaccgttcg tacgcgacaa
     1981 cgaagcggct ctcatgtggg cacacctgaa cgcgccgccg ccggcgctgt cgtcggtcag
     2041 gcccgacctt cccgccgctc tgacgccagt ccttcagcgg gccatgtcca aggatccgga
     2101 ggagcgttac gcttcgagcg gcacattcgc ggccgccctc gcggccgcag ccagcgcgac
     2161 agcgcccgca ccatcggcga tcctgacggc cgctgctgct cagaccgcgc cgacggtcga
     2221 ggcggctccg ccgatggcga cggagccccg gtcccggatt gcgccgccac gccaacgcaa
     2281 atcgcagaac cggggatggc tgccgttcct cgccggagct ctggctctcg tcctggtagc
     2341 gctggcgggc tactcggcga tgatcctgct cggggcgccc ggcggcacgg accgccctca
     2401 ggcgacgcgc ccggtaggcg ccggcccgac cccgtcaccg gcagtctcgc tcgacgtctt
     2461 gccaaccgat ggcgcgacgg tcgcgcctca gaccctcggt cccggggaca cgcccacgcc
     2521 tcgcgtcgtt cccacgcgca ccgcccggcc gacacgcagc aaccgcacgc caacgccgcg
     2581 gcaatcgacg cagcctccga ctgccactcc cacggcaacg ccgacagcca ctccgactgc
     2641 aacgccggtg gaaacgcctc gtttgcccgt cgccgctcag ggcacatggg ccgtgacata
     2701 cacgcggacc ggctcgaccg gctcgccccc gaccgatcta ggcgaccaca accggactta
     2761 tgacatcgaa ccttcgtgcg cggccgagtc cagctgccgc atgctggcta cgacgtacga
     2821 cgccggcggg gagttcgtcg gacggatcac tttccggtgg aacgggtcgg tttaccgcta
     2881 cagcggcgac gctcggtact acgcgcgcgc tggcggcagc aattgcgtga ccgaaggtgg
     2941 agaaaccatc atcggcgcgt accaaacggc tgaggtcgtc tcgatcgagc ccatgacctg
     3001 ggacggggat cgggtcatcg tcttcgacgg aaccaaacgg ataaccggca cgccaacgga
     3061 cgtcggcgcg gccaacgggt gtgagccgta ctcgcttgcg tacgacgcac gactgacgca
     3121 ctgaccgggg gttagagcag ctcctcgcga aggctggcga cgccgtcgcc gacagcagcg
     3181 gcctcagcac gctctgcgat catcgtttgg agccgggcga atcccgtctc aattcgacgc
     3241 gctcgatccg gatcgagtac cgccagcccg ttggcgactg agtcataccg ctctcgactg
     3301 cggctcaggt tgtcgagagc ggcctcgtat tcgatttcgc cgccgcccgg agcaaccgcc
     3361 tccgtgtact cgatctctac cacctcgagc agacccgccg aagcgcccat ctcatcgctg
     3421 atgatcgtcg ccgtctcctc cagatccgcc gagcgcgagc cgaccaccac tgctcccacg
     3481 gcgcccccga gcaggaagca gatgactccg actgccagaa gcagcggcca gcgggaccga
     3541 cgcgggcggt agatcgccac tcggtcagct ccttgtcacg atggccatga cgtgcgcgtc
     3601 cgacgccagc acgcactcgt tcgccacagc cgcaagtatg tcgtccagct tcgagaggga
     3661 cgtcagatca accagtcgac cgccacgcac aagcttcacc gcgggcgcca ccgacagcgg
     3721 gccgatgtgg cgaacgaggg taggcagcag ctcgtccagc tggcggtgcg cgtctgctgc
     3781 caggccaacc aggacccgtc cgcggcccgt ggcggcgata ccggcgcgct ggtcgaccag
     3841 cgcaaagtcg acgtccgaat actcgttgcg ataggcgccc actggtacga acagcgcgaa
     3901 cttgtctgcg gccaggcagt cgacgtctcg cctgagcgcg tgttcggcgg ccgcttcgca
     3961 atcggcggcg ctggtcccgt cagccagcgg cgctagtccc gccgcggtgc cggtggctgt
     4021 gacccggatc gcagctcggt cggccagacg ctcggactcg acctgaatgc tttccggagc
     4081 ggccccggca ttgatcgcct gatcttcggc ctcgcggtga agggcgctga tcagctcggg
     4141 cgtcgcttgg gtggccccgc gctcgatctc gaccctgatg agtgacaacg cgtcgccgat
     4201 cgaggagagg atctcggcat cgggcgccat gcgccattcg aggcgcagct tcgccccgag
     4261 tgtcggcagc agcgccccgg caccgccgcc gataccgacg agtaggggcg accgcagtcg
     4321 atgtccggtg acgatctcct cgacgacgtc cgcaatcttg cccgtgccga gggcgagcac
     4381 ccgccgcgcc accccatccc agggatcgcc cacgagcttg ccgactgcct cgaatgcgag
     4441 ccgtgcggct tcggtgttgc cggaagcata ggaaccgtgg gccacatcac ccagcgcatt
     4501 cgcagcgcag gtcacggtcg ccgcgaacgt tcgtccatcg gctgcctgga ccaccacgta
     4561 gtcgtcggca tctcctgcgc gtggcgcaac cagcgttgcg tgggcatcga tcaaggtttc
     4621 tggggcagcg aagcttgcgt gcgagaggcc cgcgatatgc gccgatcgcg gtccgacatc
     4681 ggacacgcgc aggcgcccca accgacgcga aacgcgcatc aggctcccac cggcgacacc
     4741 gaccacgcgg acgtccaggc tgcgaacgct ggtctgatgc tccag
//
LOCUS       DATLCO010000131         3365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_165963, whole genome shotgun sequence.
ACCESSION   DATLCO010000131 DATLCO010000000
VERSION     DATLCO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165963"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..578)
                     /locus_tag="VMZ33_03255"
     CDS             complement(207..578)
                     /locus_tag="VMZ33_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16281.1"
                     /translation="MPAPYEIPDDRSKLAVPVLATFFSVRRGRDPFFKFFMRKMFPRQ
                     VKRYEEDFGVRFLGWYNVAYGWDFDNVILLELPDYGTIDKLESDERARAVGHRAGEWM
                     FERHHAMFLRERMGPDLEYHP"
     gene            865..1524
                     /locus_tag="VMZ33_03260"
     CDS             865..1524
                     /locus_tag="VMZ33_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR16282.1"
                     /translation="MGSRETRLLRGRRRGETLTRRSLTELRVRRQDLGVSQAQLAESL
                     GYSRSQLWRLESERIDPTVRQLSEIASVLGLEVSVGLHELGDPIRDRGQQALGKRFDV
                     IPGPAWRNTAEVLLPNPGDRRSWDRLLRLTTTEPQIVGADLETRIRDIQALVRRTRER
                     ERDGHVDAVLLVLSDSATNRRLVDELRMALGPAYATSPRRNLAALREGRRLPGSGLIL
                     V"
     gene            complement(1477..1857)
                     /locus_tag="VMZ33_03265"
     CDS             complement(1477..1857)
                     /locus_tag="VMZ33_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16283.1"
                     /translation="MPEDVDPQAPSYKGEPLDAERGPGLGCFWIQVGLFVVLLVLTPL
                     SVWLSWDSRVSAVLLIVTLGLLFFVGQTTIFLLRLVAADRRSQRRPLGPNARPTVGQL
                     EEEASAQDASDEDEAGSGKPPPLP"
     gene            complement(1850..2809)
                     /locus_tag="VMZ33_03270"
     CDS             complement(1850..2809)
                     /locus_tag="VMZ33_03270"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HUR16284.1"
                     /translation="MRHLLDVEVLSDLQIARLLSLAEGYRAGDDVAAEPSLAGRHVAL
                     LFAEPSTRTRLSFELAARRLGAATYVLDPGSSSMVKGETLVDTVRNLDALGFGYLVVR
                     HHRAGAAAVAARYLLGGSVVNAGDGWHAHPTQALLDLFTLQSAFGGAGSLRGRKIAIV
                     GDVRHSRVARSNVWTLSRAGMDVWLSGPASWLHGSEGLPARVTTSLDEALDGADAVMG
                     LRVQKERMAVGVSVAEYIERYQITEARLARAAAPGAFFLHPGPINEGVEVTRAVARGP
                     RSLVLEQARNGVPVRMAVLASLVGPSGLEKAPSPVELREPALA"
     gene            complement(2840..>3365)
                     /gene="pyrR"
                     /locus_tag="VMZ33_03275"
     CDS             complement(2840..>3365)
                     /gene="pyrR"
                     /locus_tag="VMZ33_03275"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HUR16285.1"
                     /translation="RRALVRISHEIVEKHGGTDGLALLGVQRRGVPLAERLASAIESN
                     EGVRLAVGSLNITFYRDDLSKVDRQPVVNATDVPFDLSSHTVVIVDDVLYTGRTVRAA
                     LDALVDIGRPKAVRLAVLVDRGHRELPIRADHVGKNVPTSREEIIKVHLREIDGDEDE
                     VVIERGDSSAEAAA"
BASE COUNT          590 a         1138 c         1099 g          538 t
ORIGIN      
        1 gaaagcgctt cagctgatcg ccggcgtggg ccaggaaggc ggtgcgctcc atggccgcgt
       61 cggccgcgag ctcgcccagg gcacccgcca tgccgcggcc accgcctcgg ccgatgtcgc
      121 ggccaatgct ttcgctcatc gggtcctcct actgttttcg gtcgtcaagg ctgggttgcg
      181 tcttgcttct gatctggcat taatggctat gggtgatatt cgaggtcggg gcccatccgc
      241 tcccgcaaga acatggcgtg atgtcgctcg aacatccact ccccagctcg atggccgacg
      301 gcgcgggcgc gttcgtccga ctccagcttg tcgatggtgc cgtagtcggg caactcgagc
      361 aggatgacat tatcgaagtc ccagccgtat gccacgttgt accaacccag aaatcgaacg
      421 ccgaagtcct cttcatagcg cttcacctgg cgcggaaaca tcttgcgcat gaagaacttg
      481 aagaacgggt cgcggccgcg ccgaaccgag aagaacgtcg ccaggacggg cacggctaac
      541 ttcgaccggt cgtcagggat ctcatagggc gcgggcattg tcgcacgggc cgtcgagcgc
      601 accccgcgtg tgcccagcac ctgtcgccac tcgcccaacg agtacccaac tcatccagca
      661 agttgtgtca gctcgctcag cgggcattcc gtcggtcgac gtttctgacg gcggtggaag
      721 cagcctcctg cccgacctgc cgctcggtga gcgagtcatc tgccgccaga acggctatcg
      781 cgctgccatg gcgttggctt cgtcggacgc gttgctcgct gagcgagtaa gtgactgata
      841 agccggtttc gtcaggctgg tggtgtggga agtcgagaaa cacggttgtt gcggggtcgc
      901 aggcgtggag agacgttgac tcggaggtcc ctgacggaac tgagggttcg gcggcaggat
      961 cttggtgtga gccaggcaca actggccgag tcactggggt actcaaggtc gcaactttgg
     1021 cgcttggaat cggagcggat tgatccgacc gtgcgtcagc tgtcggagat tgcgtcggtg
     1081 ctgggactgg aggtatccgt cgggctgcac gagctgggcg atcccattcg tgaccggggt
     1141 cagcaggcgc ttggcaagcg ctttgatgtc ataccaggtc cggcgtggcg gaataccgcg
     1201 gaggtcttgc tgccgaaccc gggcgatcgg cgatcctggg accggctctt gcggttgacc
     1261 accaccgagc cccaaatcgt tggggccgat ctcgagactc gcatccgcga cattcaggcc
     1321 ctcgtgaggc ggacccgcga gcgcgaacgc gacggccacg tggacgcggt cttgcttgtc
     1381 ctcagcgaca gcgccacgaa ccgaagactg gttgatgagc tgcgcatggc gctgggtcca
     1441 gcgtacgcga ccagtccgcg gcggaacctg gcggcactaa gggaggggcg gcggcttccc
     1501 ggatccggcc tcatcctcgt ctgaagcgtc ctgcgcagaa gcctcctcct cgagctgacc
     1561 gaccgtcggc cgggcgttcg gcccaagcgg acgtcgctgc gagcgtcgat cggcggcgac
     1621 gaggcgcagc aggaagatcg tggtctggcc gacgaagaac agcaggccga gcgtcactat
     1681 cagcagcacg gcactgactc tcgagtccca cgacagccaa acgctgagtg gcgtgaggac
     1741 gagcagcaca acgaagagcc cgacctggat ccagaagcag cccaggccgg ggccacgctc
     1801 ggcgtcgagt ggctccccct tgtagctcgg cgcctgtggg tcaacgtcct caggcaaggg
     1861 cgggctcgcg cagttccacc ggggaggggg ccttctccaa gcccgacgga cccacgagcg
     1921 aggcaagcac cgccatccgc accggcaccc cgttgcgtgc ctgctccagc accagcgagc
     1981 gtggcccgcg cgcgacggcg cgtgtgacct cgacgccctc attgatcggc cccggatgca
     2041 ggaagaacgc acccggcgcc gcagctcgcg ccagtcgcgc ctcggtgatc tggtagcgct
     2101 cgatgtactc ggccaccgac accccgacgg ccatccgctc cttctggaca cgcaagccca
     2161 tcacggcgtc cgccccgtca agggcctcat ccagcgaggt ggtcacgcgc gccggcaacc
     2221 cctccgaccc atgaagccac gaagcagggc cgctcagcca cacgtccatg ccggcgcgcg
     2281 acagcgtcca cacgttcgag cgcgccacgc gcgagtgccg cacgtcgccg acgatggcga
     2341 tcttccgtcc acgcagggag cccgcgcctc caaaagcgct ctgcagcgtg aacaggtcga
     2401 gcagcgcctg ggtgggatgc gcgtgccagc catcaccggc gttaacgacc gatccgccga
     2461 gcaggtagcg cgctgcgacc gccgccgcgc cggcgcggtg gtgccgcaca acgagatagc
     2521 caaaccccag cgcatcgagg ttgcgcaccg tgtcgacgag ggtctcgccc ttgaccatcg
     2581 acgacgatcc cggatcgagc acgtacgtcg cggctcccaa ccgccgagct gccagctcaa
     2641 acgagagccg cgtccgagtc gacggctcgg caaagagcag cgcaacgtgc cgccccgcca
     2701 gcgaaggctc agccgcgacg tcatcgcccg ctcggtagcc ctctgcgagc gaaagcaggc
     2761 gcgcgatctg gaggtcggac aggacttcga catcaagcag atgcctcacg aggtggccac
     2821 cctcacgccg cggccaccct cacgccgcgg cctcagccga ggaatcgcca cgctcgatca
     2881 cgacttcatc ctcgtcacca tcaatctcgc ggaggtgcac cttgatgatc tcctcgcgcg
     2941 acgtgggcac gttcttgcct acgtgatcgg cccggatggg cagctcgcga tgtccacgat
     3001 cgaccagcac tgccaatcgg acggctttgg gccggccaat gtcgaccagc gcgtccagcg
     3061 ccgcgcggac ggtgcgaccg gtgtagagca cgtcgtcgac gatcaccacg gtgtgtgagc
     3121 tcaggtcgaa gggcacatca gtcgcattca caaccggctg tcgatcgacc ttggacaggt
     3181 cgtcccggta gaaggtgatg ttcaggctgc cgactgccag ccgtacgcct tcgttcgact
     3241 cgattgccga tgccagtcgt tccgcgagcg gcactccgcg gcgctgcacg cccagcaggg
     3301 cgaggccgtc ggtaccgccg tgcttctcaa cgatctcgtg gctgatgcga acgagcgcgc
     3361 ggcgc
//
LOCUS       DATLCO010000132         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_914293, whole genome shotgun sequence.
ACCESSION   DATLCO010000132 DATLCO010000000
VERSION     DATLCO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914293"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VMZ33_03280"
     CDS             complement(<1..788)
                     /locus_tag="VMZ33_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HUR16286.1"
                     /translation="MATRTPAKADAPASSSGELGADLGLTRDKLIDMYRLMAITRAVD
                     ERMWILNRAGKIPFVISGQGHEGAQVGIAYPLRQGHDWMVPYYRSVAALITFGMSPRE
                     IMLAQFARGVDPSSGGRQMPGHYGIKDRNILSVSSPVATQLLHATGIAWAAKLKKTDQ
                     VVITYVGEGSSNQGDFHEGLNFAGIHKLPVIYVVENNGYAISVPMSLQSAVEDIAIRG
                     AGYGMPGVIVDGADVLACYKAGREAIDRARRGDGPTLIEAKVTRL"
     gene            complement(797..2233)
                     /gene="lpdA"
                     /locus_tag="VMZ33_03285"
     CDS             complement(797..2233)
                     /gene="lpdA"
                     /locus_tag="VMZ33_03285"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HUR16287.1"
                     /translation="MAVGSSNSFDLIVLGGGTGGYSAAFRAGQLGLKVALIDDRSALG
                     GTCLWVGCIPTKAMLESAEMYERIQHGAADMGVNVGNIAYDYAVAAKRRDQIVSRLTK
                     GLGSLVKKNKVEHIRGLGTLHGGKKVLVKTLDENGKPDGERPLDATDVILATGSKVKS
                     LPGLVPDGKTIITSDEATTGNALPKSIVVVGGGAVGVEFASFYHDAGVEVTLLEYMPR
                     LTQLEDAEVSKELLRSFQRRGMKVVLNARFDAGDVSVDEQGVCLMVGAEGEEPKELRA
                     EQMLVATGRAPVTEGIGLESTRAKIEKGFVTVDARMRTAEPHLYACGDVIGGLLLAHV
                     AAHEGIAAVHAIAGAEAEKIDYLTMPRATYSRPQIASIGRTQEEAERDGFEVKVGKVP
                     FQAIGKALIRGEHEGFVKVVADKKTDEILGVHMIGAQVTDLISEASAAMLLEATAWEV
                     GSAVHPHPTLSEALGEAALAVDGKSINF"
BASE COUNT          379 a          786 c          692 g          422 t
ORIGIN      
        1 agccgcgtga ccttggcctc gatcagcgtc gggccgtcgc ctcgtcgcgc gcggtcaatc
       61 gcctcgcggc cggccttgta gcacgccagg acgtcggcgc cgtcgacgat cacgcccggc
      121 atcccgtacc cggcgccgcg gatggcgatg tcctcaacag ccgattggag cgacatcggg
      181 actgagatcg cgtagccgtt gttctcaacc acgtagatca ccgggagctt gtggatgccg
      241 gcgaagttca ggccctcgtg aaaatcaccc tgattagaag atccctcgcc gacgtaggta
      301 atgaccacct ggtcggtctt cttgagcttt gcggcccagg caatccccgt ggcatggagc
      361 agttgagtgg ccaccggcga cgagaccgac aggatgttcc gatccttgat gccgtaatgg
      421 ccgggcatct ggcgaccgcc ggacgatgga tccactcccc gcgcgaactg ggcgagcatg
      481 atttcccgcg ggctcatgcc gaacgtgatc agcgccgcga cggaccggta gtacggcacc
      541 atccagtcgt ggccctggcg tagtgggtag gcgattccga cctgtgcgcc ctcgtgaccc
      601 tgcccgctga tgacgaacgg gatcttgccg gcacggttga ggatccacat ccgctcgtcg
      661 acggcgcggg tgatcgccat caggcgatac atgtcgatga gcttgtcgcg tgtgagccct
      721 aggtccgcgc ccagctcacc cgagcttgac gccggagcgt ccgctttcgc aggagttcgc
      781 gtcgccatgt cgctccctaa aagttgatgg atttgccgtc aaccgcgagg gctgcctcgc
      841 cgagggcctc cgacaacgtt gggtgggggt ggacagcgga tccgacctcc cacgcggtgg
      901 cctccagcag catggcggct gacgcttcgc tgatcaaatc cgtcacctgc gcgccgatca
      961 tgtgcacgcc caggatctcg tcggttttct tgtccgccac gaccttcacg aaaccttcgt
     1021 gctcgccgcg aatgagcgcc ttaccaatgg cctggaacgg taccttgccg accttgacct
     1081 cgaagccgtc gcgctccgct tcctcctgcg tccggccgat ggacgcgatc tgcggccgtg
     1141 agtaagtcgc gcgcggcatc gtgaggtagt cgattttttc ggcctccgca ccggcgatcg
     1201 cgtgaaccgc cgcaatgccc tcatgcgcgg ccacgtgcgc gagcaagagg cccccgatca
     1261 catccccgca cgcatacaga tgcggctcag cggtgcgcat tcgcgcatcg acggtcacga
     1321 agcccttctc gatcttggcc cgggtcgact cgaggccaat gccctccgtc accggcgccc
     1381 ggcccgtggc cacgagcatc tgctcggcgc gcagctcttt tggctcctcc ccctccgcgc
     1441 cgaccatcag gcaaacgccc tgttcatcga ccgacacgtc acctgcgtcg aaccgtgcgt
     1501 tgagcactac tttcatgccg cgtcgctgga acgagcgaag aagctccttt gaaacctctg
     1561 cgtcctcgag ctgcgtcagc cggggcatgt actcaagcag cgtcacctcg acgccggcgt
     1621 cgtggtagaa gctcgcaaac tcgaccccaa ccgccccgcc gccaacgacg acgatgctct
     1681 tgggcagtgc gttgccagtt gtggcctcgt ccgaggtgat gatcgtcttg ccgtcaggca
     1741 cgagtccggg caaggacttc accttcgagc ccgtggccag gatgacgtcc gtagcgtcca
     1801 gtggccgctc accatcgggc ttgccgttct cgtcgagcgt cttgaccagg accttcttgc
     1861 cgccgtgcag ggtgcccaac ccgcgaatgt gctcgacctt gttcttcttg accagcgagc
     1921 cgaggccctt ggtcagccgc gacacgatct gatcgcggcg cttggcggca acggcgtagt
     1981 cgtaggcgat gttgcccaca ttcacgccca tatcggcggc gccgtgctgg atgcgctcat
     2041 acatctcagc ggactcaagc atcgccttgg tcgggatgca accgacccac aggcacgtcc
     2101 caccgagtgc cgaacggtcg tcgatcaggg cgaccttgag gcccagttgg cccgcgcgaa
     2161 atgcggccga atagccgcct gtgccgccgc cgagaacgat cagatcgaac gaatttgacg
     2221 aaccgactgc cacgtcgcca gcctacactg tcggcgcccg cggacggcct ttgcacgct
//
LOCUS       DATLCO010000133         4213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_915357, whole genome shotgun sequence.
ACCESSION   DATLCO010000133 DATLCO010000000
VERSION     DATLCO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915357"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VMZ33_03290"
     CDS             complement(<1..1207)
                     /locus_tag="VMZ33_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUR16288.1"
                     /translation="MTDPELTITLAASGFDQPTGIAFIGDNDYLVIEKATGLVRRAQA
                     GQVAEQPVLDLGVNYFDERGLLGIALHPAFAENGYVYLYWTAHDDSAPPDSLLGEDTD
                     EATQVPELGNRVDRFVWDGTALTWDQEIVALRSNTLETDTSDRIRGNHDAGPLEFGPD
                     GMLYVQIGDQNLRGQLQNITDGPAPDDPNLTGIILRLNDDGSVPDDNPFFAQGADMGD
                     AIGENIQMIWAYGIRNSFGLRFHPQTGDLWQTENGDDSWDEINRFTPGANSGWIQVMG
                     PPERFDEYRQIEVDSEDGLDNPDYPPDQLAENVDEAQGRLFALDGSAYSAPAFAWRYP
                     PAVTALEFVTGEELGASSANSLFLGTVLTDSLLRYPLANDGSGFAFEGGLADNVDDNA
                     AKGDLGESAD"
     gene            complement(1789..2034)
                     /locus_tag="VMZ33_03295"
     CDS             complement(1789..2034)
                     /locus_tag="VMZ33_03295"
                     /inference="COORDINATES: protein motif:HMM:NF041742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGxxGxxG family protein"
                     /protein_id="HUR16289.1"
                     /translation="MRNKILALVAAAAFSVAGAGAVLGQSPDATTRPTTTPTEQTNKD
                     DGFPIGLLGLAGLLGLAGLMRKEREPRTVVTDANVRR"
     gene            complement(2164..2592)
                     /locus_tag="VMZ33_03300"
     CDS             complement(2164..2592)
                     /locus_tag="VMZ33_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16290.1"
                     /translation="MRRLIPALVVLLVLLLAPAAVSAHEISRSCVISVEPGTGGTRDI
                     YRITGRHFPHEVNGGGLEVRIDVSRVVFDHHGSSHLEMKQILILFLIPTTHRFYVDFN
                     APEPGAVQPWLKPGHYVVAAQTPHQPGCRTVSGFDVRRFG"
     gene            complement(2708..3163)
                     /locus_tag="VMZ33_03305"
     CDS             complement(2708..3163)
                     /locus_tag="VMZ33_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HUR16291.1"
                     /translation="MTSSGSHRQTLAGAVPEIKAELRLRGQRWTPQRRLILEVLEQTS
                     GHVTGSELVELCREREPDVTPSTVYRTLDVLEQLGYVSHSHSARGREEYHVLSKAEHA
                     HLECRNCGRMLELSRAEADEITQVVEQRHGFVADLGHMTIVGLCSECAS"
     gene            3223..>4213
                     /locus_tag="VMZ33_03310"
     CDS             3223..>4213
                     /locus_tag="VMZ33_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HUR16292.1"
                     /translation="MKTPLSRTFLIALAFALAACTGGAAQTATTSGTSLGWYDELTSG
                     TDKLDVATTVAPISSLARNIGGNRIRLHGIVPDATNSHTFEPSPTDARHLAAADLIIV
                     NGLHLEQPTLDLAEASKRPETPVKQLGDNTITRDEWLFDFSFPESAGDPNPHLWMNVE
                     YARRYSELMRDWFSAADPANADYFAGNFQALKARLDLLDALIRQSTDSVPEANRKLLT
                     YHDSWAYWAREYGFTVVGAVQASDFSDPSPQEVARLIDQVKAEHVPAVFGSEVFPSPV
                     LEQIAREAGAEFVDQLSDDEPPGPEKSADHTYYGMLKKDMQIMVEALGGNASVF"
BASE COUNT          750 a         1332 c         1369 g          762 t
ORIGIN      
        1 aatcggcgct ttcgccgagg tcgcccttgg cggcgttatc gtcgacgttg tcggccagac
       61 cgccttcaaa ggcgaacccg gagccgtcat tggcgagcgg atagcgcaga agcgaatccg
      121 tcaggaccgt cccgaggaaa agcgagttag ctgatgaggc gccgagttcc tcgccagtga
      181 cgaactcgag cgctgtcacg gccgggggat agcgccacgc gaacgccggc gcggagtagg
      241 cgctgccatc gagcgcgaac agccgtccct gggcttcgtc gacgttctcc gccagctggt
      301 cgggcggata gtcgggattg tccaagccgt cctccgagtc gacctcgatc tgtcgatatt
      361 catcgaagcg ttctggtgga cccatgacct ggatccagcc ggaattcgcc cccggggtga
      421 aacggttgat ctcgtcccac gaatcgtcgc cgttctccgt ctgccacagg tcaccggtct
      481 gcgggtggaa gcgcagcccg aagctgtttc gaatgccgta tgcccagatc atctggatgt
      541 tctcgccgat tgcatcgccc atgtccgcgc cctgcgcgaa gaacgggttg tcgtcgggca
      601 ctgagccgtc gtcgttcaac cgcaggatga tgccggtgag gttcgggtcg tcgggcgcgg
      661 gcccgtcagt gatgttctgg agctggccgc gcaggttctg gtcgccgatc tggacgtaaa
      721 gcatcccgtc gggaccgaat tcgagcggtc cagcgtcgtg gttgccacgg atacggtcgc
      781 tagtgtcggt ttcgagcgtg ttcgatcgca gcgcgacgat ctcctggtcc cacgtcaacg
      841 cggtgccatc ccaaacgaag cgatcgacgc gattgcccag ctcgggcact tgcgttgctt
      901 cgtcggtgtc ctcgccgagc agggagtctg gtggagccga gtcgtcgtgt gccgtccagt
      961 agagatagac gtacccgttc tccgcgaacg ctgggtgcaa cgcgatgccg agcagtccgc
     1021 gctcgtcgaa atagttgacg cccaaatcga gcaccggctg ctcggcaacc tggccggcct
     1081 gagcgcgacg cacgagccca gtcgccttct cgatcacgag gtagtcgttg tcaccgatga
     1141 aggcgatccc cgtcggctgg tcgaagccac tggccgcaag agtgatggtc agctcgggat
     1201 cggtcatcgt cggcatgacc gcggcgcccg gcgtcgcgtc tggcgactcg ccggtggtcg
     1261 gagctgtgct cgggctcgaa gcggagccgg ttggcgaggt gccttgggag gcggcttcgc
     1321 tggcgcccgg agcggcagtt gaggagctcg gcttcgcgcc gctggggatc gcacctgacg
     1381 ttgactgtcc aaacgaggtg ccgggactcg ccgacgggga tccgcacgcg gctacaagga
     1441 tgatggccgt ggcggcggca atagaggacg tcagtcgcgg catcgaatca tctccaggcg
     1501 ctgcccgctg cggcagacca cggcgccgca gggaggaaca gtcgttccaa cctatgcccg
     1561 tgcgatgctg ctctgaggcg ctcgaaggcc cgcgacgcct atcggttgcg tgacagcagg
     1621 cgcctggctc gctgatgcgc ccgcgtcgtc gcgagcttgg cctcgagcct caactggtgg
     1681 acgccgaggt gatgggcttg ctgcgcggct taagactgcg ctcagagatc gacgggaatc
     1741 aaaaggcctg gctcacgtgc tcgttgcacg gagccaggcc acttagggtt atcgccgaac
     1801 gttggcgtcg gttacgacgg tccgcggctc gcgctccttg cgcatgaggc ctgcgaggcc
     1861 gagcaaaccc gcgaggccaa ggaggccgat cgggaacccg tcatccttgt tcgtctgctc
     1921 ggtcggcgtc gtcgtcggcc gagttgtcgc atcgggggac tggccaagca cggcaccggc
     1981 gccggcgaca ctgaaggcgg cggccgcgac cagcgccaga atcttgttac gcatgtattg
     2041 actccatcgt gagtttaagc ggtcaaagcg accgcaggct caacgaccat acgtaagcag
     2101 cgcctcgtaa ctgtcgagtc ggagggctaa agactctttc ggttcgcttg cagcgcgtcc
     2161 cgtctatccg aagcgtcgga cgtcgaaacc gctgacggtc cggcaacctg gttggtgtgg
     2221 tgtctgtgcg gctacgacgt agtgacccgg cttcagccag ggctgaacag cgcctggctc
     2281 gggcgcgttg aagtcgacgt agaaccggtg ggtggtcggg atgaggaaca agatcaggat
     2341 ctgcttcatt tcgaggtgcg acgaaccgtg gtgatcgaat acgacgcgtg acacatcgat
     2401 tcgcacttcg agaccgccgc cgttgacctc atgcggaaag tgccggccag tgatgcgata
     2461 gatgtcgcgc gtgccacctg taccgggttc aaccgagata acgcagctgc gactgatctc
     2521 gtgggcgctg actgccgcag gggctagcag caagacgaga aggacaacga gggcaggaat
     2581 gagtcggcgc accgtcttct ccaaagttgg ggcccgacta acgggcccgt ggatgacgcg
     2641 tgttgggctc agcgttaccc ggcataacgc ccgccgcgcc gatccggttc cctcccagcg
     2701 aacctgttta gctcgcgcac tccgaacaca gtccgacgat cgtcatgtgg cccaggtccg
     2761 cgacgaaccc atggcgctgc tcgacgacct gcgtgatctc gtccgcttcc gcgcgggaca
     2821 gctccagcat gcgcccgcag ttccggcact ccaaatgagc gtgctcagcc ttcgatagca
     2881 cgtggtattc ctcacggccc cgcgctgagt ggctgtgact cacgtaaccg agctgctcga
     2941 ggacgtcgag cgtccgatag acggttgacg gggtcacgtc gggctcgcgc tcgcggcaca
     3001 gctccacgag ctcgctgccg gtgacgtggc cggacgtctg ctcgagaacc tcgaggatca
     3061 gacgacgctg cggggtccat cgctgccctc gcaatcgcag ctcggccttg atctcaggca
     3121 cggcgcccgc cagggtctgc cgatgtgatc cagacgacgt catgtttcga actatacaaa
     3181 catgaagcag ttgcaatagg ttgcattaga cttgctgaac cggtgaaaac gcccctctcg
     3241 cgaacctttc tgatcgccct ggcctttgcc cttgccgcat gcaccggcgg tgcagcgcag
     3301 acggcaacta ccagtggaac gtcgctgggc tggtatgacg agttgacgag tggcacggac
     3361 aagcttgatg tggccaccac cgtggcgcca atcagctcgc tggcgcgcaa catcggcggg
     3421 aatcgcatcc gcctgcacgg gatcgtgcct gacgccacga actcacacac cttcgagccg
     3481 agtcccacgg atgcgcgcca cctcgccgcc gccgatctca tcatcgtcaa cgggctccat
     3541 ctcgagcaac ccacgctcga cctggccgaa gcgtccaagc ggcccgagac gccggtcaag
     3601 cagctcggcg acaacaccat cacccgcgac gagtggctgt ttgacttcag cttcccggag
     3661 tctgcgggcg atccgaaccc ccacctctgg atgaacgtgg agtacgcccg ccgctactca
     3721 gagctgatgc gcgattggtt cagcgcggca gatcctgcaa acgccgacta cttcgctggc
     3781 aatttccaag ccttgaaagc ccgcctcgac ctcctggatg ccctcatccg ccagtccacc
     3841 gattcggtcc cggaggcaaa tcggaagctg ctgacctacc acgactcgtg ggcctactgg
     3901 gcgcgcgaat acggctttac cgtcgttggt gcggtgcagg catcggattt cagtgacccc
     3961 agcccgcagg aagtcgcccg attgatcgac caggtgaagg ccgaacacgt gccggcagtt
     4021 ttcgggtcgg aggtctttcc cagcccggtg ctcgagcaga tcgcgcgcga ggcgggcgcg
     4081 gagttcgtgg accagctgag cgacgacgag ccgcccgggc ctgaaaaatc ggccgatcac
     4141 acctactacg gcatgctcaa gaaggacatg cagatcatgg ttgaggcgct cgggggcaac
     4201 gcttcggtct tca
//
LOCUS       DATLCO010000134         3771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6048209, whole genome shotgun sequence.
ACCESSION   DATLCO010000134 DATLCO010000000
VERSION     DATLCO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6048209"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="VMZ33_03315"
     CDS             <1..2181
                     /locus_tag="VMZ33_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUR16293.1"
                     /translation="GHYLADYGWLTGLDAQGVTERIVADLRERGFFYRLESYTHRYPH
                     CWRCGTALLFRVVDEWYISMGEYYDKPRGEVTAEEKDRSLRYQIMDVVDQIRWIPGFG
                     YERELDWLRTMSDWMISKKRYWGLALPIWECADCGAFDVVGGRDELRQRAVSGWEEFE
                     GHTPHRPYVDAVKVACKSCGAATSRIKDVGNPWLDAGIVPFSTLHYRTEPDYWSNWFP
                     ADFITESFPGQFRNWFYSMLAMSTVLRNEPPFKTIFGYATLFAEDGRAMHKSWGNAIE
                     FDEAAERMGVDVMRWMYAKHKPEDNILFGYGTADAARRELLILWNVYAFFTTYARLAG
                     WAPRRGIDEEIRAAGEWPILDRWILSRAAGLATDVGQELTDYDAMGATRLISTFIDDL
                     STWYLRRSRDRMRQTASEQDRNTALATLHAALASLARSVAPILPFLAEELYQNLMVGP
                     IPAGSVPESVHLTDWPADAMAEHRDEAVEGAMDTLRRAVELVRTLRGQQNIRLRQPLA
                     TMWLALPGGRLGDISAEDERALLELLADEVNVRRVEVIGNESDLVERRVKPLLPVIGP
                     RLGSQIPRVMAAARANEVEYLPEGRVRLGGVELAPTEVEIIATPRPGTAVAHDEGVVV
                     VIDTEIDDSLRAEGDAREVARAVQDLRKQAELELDDQIELWLSGPAQLVATLEPHLGQ
                     VARDTIASAVHREAAPSDVSASVVSLSGGDLTLAIRRVEGANGR"
     gene            2171..2713
                     /gene="lspA"
                     /locus_tag="VMZ33_03320"
     CDS             2171..2713
                     /gene="lspA"
                     /locus_tag="VMZ33_03320"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HUR16294.1"
                     /translation="MVDRRRWYRRWATFFALAGVVIVIDQVTKQYVDMNFPVASVHAA
                     PGLAEPTPIIDGIVRIAKTYNTGGIFGFFGDSAVVLALASTVVIALIVIYQWREGVRG
                     PWQLTVALGLLLGGALGNFIDRIRLQAVVDFVDMGLGDWRWYTFNVADAAISMALLLL
                     IVMSVFGDRFTTRVAAQPQD"
     gene            2789..3736
                     /locus_tag="VMZ33_03325"
     CDS             2789..3736
                     /locus_tag="VMZ33_03325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUR16295.1"
                     /translation="MRPTVESETFTLTVPLSDKPRRADRVVADASGLSRSYVQRLIEE
                     GRLTQNGQPVKSNTILESGSSLKLEVPEIRVAALEAEDVPLTIVYEDADVLVIDKPSG
                     LVTHPSPGHSAGTLVNALLARGGVEAYGTVAGSERPGIVHRLDRDTSGLILVARNDAA
                     QTALMTQLKARRVKKTYLALVEGAVPAEVGRIEAPIGRDPKRFGRMAVVSTGRPSITG
                     YRVRERLAGWTLLDVDLHTGRTHQIRVHLASIGHPVAGDPVYATGAARRGPDGLARLF
                     LHSWRIEFVSPGQEAIVRAEAPLPAELEQVLSTLRSPRP"
BASE COUNT          639 a         1213 c         1269 g          650 t
ORIGIN      
        1 gggcactatc tggcggacta cggctggctg acgggcctcg acgcccaggg tgtgaccgag
       61 cgcatcgtcg ccgacctcag ggagcgcggc ttcttctatc gcctcgagtc gtacacgcac
      121 cgctacccgc attgctggcg ctgcggcacg gcgctcctct tccgagtcgt ggatgagtgg
      181 tacatctcca tgggcgagta ctacgacaaa ccgcgcgggg aagtgacggc cgaggagaag
      241 gaccgcagct tgcgctacca gatcatggat gtcgtcgacc agattcggtg gatcccgggc
      301 ttcggctacg agcgcgaact cgactggctg cggacgatgt ccgactggat gatcagcaag
      361 aagcgctact ggggtctggc actgccgatc tgggagtgcg cggattgtgg tgcgttcgat
      421 gtcgtcggcg ggcgcgacga gctgaggcag cgcgccgtct caggctggga ggagttcgaa
      481 ggccacacgc cgcaccgccc gtacgtagac gccgtcaagg tggcctgcaa atcgtgtggc
      541 gcggcgacgt cacgcatcaa ggacgtcggc aacccatggc tcgatgcagg catcgtgccc
      601 ttctccaccc ttcactaccg gaccgagccc gactactgga gcaactggtt cccggccgat
      661 ttcatcaccg agtcgttccc cggccaattc cgcaattggt tctactcgat gcttgcaatg
      721 agcacggtgc tgcggaacga gccgccgttc aagacgatct tcggctacgc cactcttttt
      781 gccgaagacg gacgcgcgat gcacaagtcg tggggcaacg cgatcgagtt cgacgaagca
      841 gcggagcgca tgggcgtcga tgtcatgcgc tggatgtacg ccaagcacaa gcccgaggac
      901 aacatccttt tcggctacgg caccgcggac gctgcgcggc gcgagctgct catcctctgg
      961 aacgtctacg ccttcttcac gacgtacgcc cggttggccg gctgggcgcc gcggcgcggg
     1021 atcgacgagg aaattcgggc tgccggggag tggccaatcc tcgaccgctg gatcctatcg
     1081 cgtgccgcgg gactggccac ggacgtaggg caggagctga ccgactacga cgcgatgggc
     1141 gccacgcggc tcatcagcac gttcatcgac gacctctcga cgtggtatct gcgccggtcc
     1201 cgcgaccgga tgcgccagac agcttctgag caagaccgca acacggccct ggcgacgctt
     1261 cacgcggcgt tggcgagcct cgcgcgttcg gtcgcgccaa tcctgccgtt ccttgccgag
     1321 gagctgtacc agaacctcat ggttgggcct atcccggccg gcagcgtgcc cgagagcgtg
     1381 cacctgaccg actggccggc cgatgccatg gccgagcacc gcgacgaagc cgtcgagggg
     1441 gcgatggaca ctctccgtcg ggccgtcgaa ctggttcgta cgctccgtgg ccagcagaac
     1501 atccgtcttc ggcaaccact cgccaccatg tggctggcac tccccggcgg ccggctgggt
     1561 gacatctcag ccgaggatga acgagctctg ctcgagctgc tcgccgacga ggtcaacgtc
     1621 aggagagtgg aggtaatcgg aaacgagtct gacctggtcg aacgccgtgt gaagccgctc
     1681 ctgcccgtga tcgggccgcg gctgggctcg cagatcccac gggtgatggc cgcagcacgc
     1741 gcgaacgagg tcgaatacct ccccgaaggc cgcgtgcgcc tgggcggtgt ggagctcgcg
     1801 ccgaccgagg tcgagatcat cgccacgcct cgcccgggca cggccgtggc tcacgacgag
     1861 ggcgtggtcg tggtcatcga caccgagatc gacgacagtc tgcgcgcaga gggcgacgcc
     1921 cgcgaggtcg ctcgcgccgt gcaggatctg cgcaagcagg ccgagctcga gcttgacgac
     1981 cagatcgagc tgtggctctc agggcccgcc caactcgttg cgacccttga gccccatctc
     2041 gggcaggttg cgcgcgacac aatcgcctcg gccgttcatc gcgaggcagc gccctccgac
     2101 gtttctgcca gcgtcgtcag cctgtctggc ggcgacctga cgctcgcgat ccgtcgagtc
     2161 gagggcgcaa atggtcgata gacggcgctg gtacaggcgc tgggcaacat tctttgcgct
     2221 ggccggggtg gtaatcgtta tcgatcaggt caccaagcag tacgtcgaca tgaacttccc
     2281 ggtcgcttca gtgcatgccg ccccggggct ggctgagccg acgccgatca tcgacggcat
     2341 cgtgcgcatc gcgaagacct acaacaccgg tggcatcttc ggattcttcg gcgattcagc
     2401 ggttgtgctc gccctcgcca gcaccgtggt cattgcgctg atcgtgatct accagtggcg
     2461 tgaaggtgtg cgcggtccat ggcagctcac ggttgcactt gggctgctgc tcggtggcgc
     2521 gctgggcaac ttcatcgacc gcatcaggct ccaggcggtg gtcgatttcg tggacatggg
     2581 cctcggcgac tggcgctggt acacgttcaa cgttgccgac gcggcaatca gcatggctct
     2641 gctactgctg atcgtgatga gcgtgttcgg cgatcggttc accacacgag tcgcggcaca
     2701 gccgcaggac tagatgtccg aaccgcaagg aaggcggcag ggggacgccg tcaggcgcct
     2761 ctctgacaac gaccacgcaa cagcgcccgt gaggccgacc gtggagtccg aaacattcac
     2821 gctgacggtg ccactctcgg acaagccgcg tcgcgcggac cgcgtcgtcg cggatgcctc
     2881 ggggctgtcg cgcagctacg tgcaacgcct catcgaggaa gggcggctga cccagaatgg
     2941 gcagcccgtc aagagcaata cgattcttga gtccggcagc agcctgaagc tcgaagtccc
     3001 cgagatccgc gtggctgcac tcgaggctga ggatgtaccg ctcacgatcg tgtacgagga
     3061 cgctgacgtg ctcgtcatcg acaagccgtc ggggctcgtg actcatccgt cgccgggcca
     3121 ttcggcgggc acgctcgtca acgcgctcct cgcccgtggg ggagttgagg catacggcac
     3181 cgttgctggc tcggagcggc ccggcattgt tcatcggctc gaccgcgata ccagcggcct
     3241 gatcctcgtc gcgcgcaacg acgcggcgca aacggcgctc atgacgcagc tgaaggcacg
     3301 tcgggtgaag aagacgtacc tggctcttgt cgagggagcc gtcccggctg aagtcggtcg
     3361 catcgaggcg cctatcggtc gggaccccaa gcgatttggc aggatggcgg tggtgtctac
     3421 aggccgcccg tcgattactg gctatcgcgt acgcgagcga ctcgccggct ggacgctgct
     3481 cgacgtcgac ctgcacaccg ggcggacgca tcagattcgc gtgcatctcg cgtcgatcgg
     3541 ccatcccgtc gcgggtgatc cggtatatgc gacgggggcc gcgcgacgcg ggccggacgg
     3601 cctcgctcgc ctgttcctgc actcgtggag gattgagttc gtgtcccccg gtcaagaggc
     3661 tattgttcgg gcagaggcgc cgctgccggc ggagttggag caggtgttga gtacgttgag
     3721 gagtccgcgc ccatgaccga aacggcggtc gctgagccgg ccgagacccc t
//
LOCUS       DATLCO010000135         4916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10520955, whole genome shotgun sequence.
ACCESSION   DATLCO010000135 DATLCO010000000
VERSION     DATLCO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10520955"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..605)
                     /gene="ruvA"
                     /locus_tag="VMZ33_03330"
     CDS             complement(18..605)
                     /gene="ruvA"
                     /locus_tag="VMZ33_03330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HUR16296.1"
                     /translation="MIAFVEGTIVTIAGDSVIVEAGGVGYRLFCGPGTLAGLKEGATH
                     RLYTHHLVREDQQALYGFRTTEELAFFEMLTTVTGVGPKVALAIVSARPVSDLQLAIF
                     QGDEGVLTAVSGVGKKLAARIVLELKEKVSAAGAASGAGPGASAESEIVGALQALGYT
                     ASEARSAATAALSALPVGASLEERVKASLRILRRD"
     gene            complement(624..1199)
                     /gene="ruvC"
                     /locus_tag="VMZ33_03335"
     CDS             complement(624..1199)
                     /gene="ruvC"
                     /locus_tag="VMZ33_03335"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HUR16297.1"
                     /translation="MRTWTTSSASRPTLTCQARSWPRPPRLDHGRAALIVLGIDPGTA
                     QTGYGIVERSGSKLRMVDYGCVETLSTMELPQRLLEIHSAVLELIDTHHPTSVGVERL
                     FFNRNVMTAFAVGQARGVVLLAAAQRGLPVSEYGPHEVKMAVTGYGRAPKDQVQRMVQ
                     LVLGMHVLPRPDDAADALAVAICLAHGQRPD"
     gene            complement(1117..1869)
                     /locus_tag="VMZ33_03340"
     CDS             complement(1117..1869)
                     /locus_tag="VMZ33_03340"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HUR16298.1"
                     /translation="MSGHSKWSSIKRQKGVNDAKRGAIFTKIAREVTIAAREGGGDPD
                     ANYRLRLAIEKARSENMPADNIKRAIEKAAGGTAAEQYEELIYEGYGPGGVALLVEAA
                     TDNKNRTAADVRALFTKAGGSLAGGGAVAWQFEPRGVVAVDRNGIDADDVALMAIDAG
                     ADDVDTDADPIEMVTDPGNLESLRKGLEAAGIKVDSAEVTMQPKTTIEVDTHVARQNL
                     RLIEMLEDLDDVQRVTANFDLPSEILAEAAAS"
     gene            1896..3971
                     /gene="fusA"
                     /locus_tag="VMZ33_03345"
     CDS             1896..3971
                     /gene="fusA"
                     /locus_tag="VMZ33_03345"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HUR16299.1"
                     /translation="MTTDMSRLRNVVLAGHSDSGKTTLAEHLLHATGAISRLGRVDDE
                     TASLDFEPEEHKRKQSLSLAAATLSHEGHQITLIDTPGYADFAAEVVQGFAAADAALL
                     TMDASGGVEAGTERAISLGRTTGTAAIFVITRCERENADPVAALDALRAAFGNKIAPL
                     HLAIGKADTFRGYVDLVHRKAYIFDKGKRTETGIPADMADEVARRRDQLLEAAAEADD
                     DVLTKYLEGVEISDQELDACLHRGVRDSILAPVMVVSAAHDIGIDGVLDAMVRYFPSP
                     DEENPAKALDGAGKEVEVPPDANGPLLARVFKTTADPFVGRLTYFRVYSGKIGSHDHI
                     WNANRGEEERIGQVLRVKGKDTEPVGSIGAGEIGAVAKLVHTHTGETLSSREKPFRLP
                     DIEFPRASLGVAIEPETKADLDKMGAALGRLVEEDPGVRLEREVETGEQLLWAQGESH
                     VAVIVERLKRKFGAAVLTRQPRVPYRETIRSRTQVEGRHKKQTGGRGQFGHVWLEIEP
                     NPESGVQFATRIVGGAVPRQFWAGVEKGVHDIAQKGPIAGYPVIDFKATLYDGSFHTV
                     DSDELSFRMAGQLATRKGIQDASPALLEPIMDVKVRVPEEYMGDVNRDLNTRRGHVLG
                     MDSSDGMQVVHAQVPQSELFTYATELRSLTGGRGMFSATLDHYQEMPAHVAQKVIEEH
                     KKDEAASGH"
     gene            complement(4203..4727)
                     /locus_tag="VMZ33_03350"
     CDS             complement(4203..4727)
                     /locus_tag="VMZ33_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16300.1"
                     /translation="MTRYIPRFAAIIVALAIMVAATGVVLAATSAVTGNAQKQIKVVR
                     ENTTTASATSNSTTYSDIPGATVSMTVPSSTNALLVARFQSHGSVIQTSGCIVRILVG
                     ATTMEPNGNYPFMGQANVSESVAGSGAIERSLAVGPGTYTVKAQLRLATANSVNSQCQ
                     LSGWHFAVERHARS"
BASE COUNT          863 a         1618 c         1595 g          840 t
ORIGIN      
        1 gtcgttccta gttgtggtca gtcccgacgc aggatacgca gcgacgcctt gacgcgctcc
       61 tcgagcgacg cgccgacggg caacgcggac agggccgccg tggctgcgga acgcgcttcg
      121 gacgcggtgt agccgagggc ctgcagcgca ccgacgatct ccgattcggc ggaagcgccc
      181 ggtccagcac cgctggcggc tcctgctgcc gagaccttct ccttgagctc gagcacgatt
      241 cgggcggcga gcttcttgcc cacccctgac acggccgtca acacgccttc gtcgccctgg
      301 aaaatcgcca gctggaggtc cgaaacgggc cgcgccgaca cgatcgcgag cgcaaccttg
      361 ggaccgacgc cggtcactgt cgtcagcatc tcgaagaatg ccagctcttc tgtcgtccga
      421 aagccgtaga gcgcttgctg atcctcacgc acgagatggt gcgtgtagag ccgatgagtc
      481 gcgccctcct tcaagcccgc gagcgtgccc ggcccacaga agaggcggta tccaacgccg
      541 cccgcctcga cgatcaccga atcgcccgcg atggtcacga tcgtgccctc gacgaaggca
      601 atcaccggct aacgactgtc taactagtcg ggacgctggc catgggccag gcagatggcg
      661 acggcaagcg cgtcggccgc gtcgtcgggc cgcggtagca cgtgcatgcc gagcaccagc
      721 tgcaccatgc gctgaacctg gtccttgggg gcgcggccat agccggtgac ggccatcttg
      781 acctcgtgcg ggccgtattc ggataccggc aagccacgtt gggcggccgc gagaaggacc
      841 actccccttg cctgtccgac ggcgaacgcg gtcatgacat tgcggttgaa aaagagccgc
      901 tcgaccccaa ccgacgtcgg atgatgcgtg tcgatcagtt ccagcaccgc gctgtgtatc
      961 tccagcaggc gttgcggcag ctccatcgtc gaaagcgtct cgacacagcc gtaatcgacc
     1021 atgcgcagct tcgagccgct gcgctcgacg attccgtaac cggtctgggc agtcccggga
     1081 tcgatcccga ggacaatcaa ggctgccctg ccgtggtcaa gacgcggcgg cctcggccag
     1141 gatctcgctt ggcaggtcaa agttggccgt gacgcgctgg acgtcgtcca ggtcctcaag
     1201 catctcgatc agccgcaggt tctgacgcgc gacgtgcgtg tcgacctcga tggtcgtctt
     1261 gggctgcatc gtgacctcgg ccgaatcgac cttgatgccc gccgcctcga ggcccttgcg
     1321 aagtgattcg aggttgcctg ggtcggtcac catctcgatc gggtcagcgt ccgtgtcgac
     1381 atcgtcggcc ccggcgtcga tggccatcag cgccacatcg tccgcgtcga tgccattgcg
     1441 atcaacggcg accacgccgc gcggctcgaa ctgccaggca acggcaccgc caccagcgag
     1501 cgagccgcca gccttggtga acagcgcacg gacatccgcg gcagtgcggt tcttgttatc
     1561 ggtcgccgct tccacgagaa gtgcaacgcc accggggccg tagccctcgt agatcagctc
     1621 ctcgtactgc tcggccgcag tgccaccagc ggccttctcg atcgcgcgct tgatgttgtc
     1681 ggcgggcatg ttctccgagc gtgccttctc gatcgcaagg cggagccgat agttggcgtc
     1741 agggtcgccg cctccctcgc gcgcggcaat ggtgacttca cgcgcaatct tggtgaagat
     1801 ggcgccgcgt ttggcgtcat tgacgccctt ctgacgcttg atcgaagacc atttcgagtg
     1861 cccggacatc gcgaccgatt ctatatgctg cgggcatgac cacagatatg tcgcgccttc
     1921 gtaatgtggt tctcgccggt cattccgact ccggaaagac gacgctggcc gagcacctcc
     1981 tgcacgccac cggcgccatc tcgcgtctcg gccgggtgga cgacgaaacc gcctcgctcg
     2041 atttcgagcc agaggagcac aagcgcaagc aatccctctc gctggctgcc gcgaccctgt
     2101 cccacgaggg acaccagatc accctgatcg acacgccggg ttacgcggac ttcgctgcgg
     2161 aggtggtcca ggggttcgcc gcggctgacg ccgccctgct gacgatggat gcctcgggtg
     2221 gcgttgaagc aggtaccgag cgggcgatca gcctcggccg cacgacgggg acggcggcaa
     2281 tcttcgtcat cacacgctgc gagcgcgaga acgccgatcc cgtggctgcg ctcgacgcgt
     2341 tgcgcgctgc cttcggcaac aagatcgcgc cactccacct ggccatcggc aaggccgata
     2401 cgttccgggg ctacgtggac ctcgtccatc gcaaggccta catcttcgat aagggcaagc
     2461 gcaccgagac gggtatcccg gccgacatgg ccgacgaggt cgcccgacgc cgcgaccagc
     2521 tgctcgaagc ggctgccgag gccgacgacg acgtcctgac gaagtacctc gagggcgttg
     2581 agatcagcga ccaggaactc gacgcctgcc tccaccgtgg cgtgcgtgac tcaatccttg
     2641 cgccggtcat ggtcgtgtcg gccgcgcacg acataggcat cgacggcgtc ctcgacgcga
     2701 tggtgcggta cttcccctcg cccgacgagg agaacccggc caaggctctc gatggcgccg
     2761 gcaaggaagt agaggtgccg cctgacgcca acggtccgct ccttgcccgc gtcttcaaga
     2821 cgaccgccga cccgtttgtc ggccggctga cctacttccg tgtctatagc ggcaagatcg
     2881 gctcgcatga ccacatctgg aacgcgaacc gaggcgagga agagcgcatc ggccaggtac
     2941 tgcgcgtcaa gggcaaggac actgagcccg tggggtcgat tggcgccggc gagatcggcg
     3001 ccgtggcaaa gctcgtgcac acgcacacgg gcgagaccct gtcgtcgcgc gaaaagccgt
     3061 tcaggctgcc tgacatcgag ttcccgcgcg cgtccctggg cgtggcgatc gagcccgaaa
     3121 ccaaggccga cctcgacaag atgggcgccg ctctcggcag gctcgttgag gaggatcccg
     3181 gcgtacgcct tgagcgcgag gtcgaaacgg gcgagcagct gctgtgggcg cagggtgagt
     3241 cccacgtggc cgtcatcgtc gagcgcctga agcgtaagtt cggcgcagcc gtgctgacgc
     3301 gccagccgcg cgtgccgtac cgcgagacga tccgctccag gacgcaggtt gaaggccggc
     3361 acaaaaagca gaccggcggg cgcggccagt tcggccacgt ctggctggag atcgagccga
     3421 atcccgaatc gggagtccaa ttcgccacgc gaatcgtcgg cggcgccgtg ccgcgtcagt
     3481 tctgggcggg cgtggagaag ggcgtgcacg acatcgctca gaagggtccg atcgccggct
     3541 acccggtaat cgacttcaag gcaactctgt acgacgggtc gttccacacg gttgactcgg
     3601 atgagctctc gtttcgcatg gccggccaac tggcgacgcg caagggcatc caggacgcct
     3661 caccggcgct gctcgagccg atcatggacg tcaaggtccg cgtgcctgag gagtacatgg
     3721 gcgacgtgaa ccgcgacctc aacacgcgcc gcggccacgt tctggggatg gactcttccg
     3781 acggcatgca agtcgtacat gcccaggtgc cgcagtcgga gctgttcacg tacgccaccg
     3841 agctgcgctc gctgactggc gggcggggca tgttcagcgc cacgctcgat cactaccagg
     3901 agatgcccgc ccacgtggca cagaaggtga tcgaggagca caagaaggac gaggccgcct
     3961 ccgggcattg accttgaccg gccggtggac gcgtttcgtg tcgacgagac accacctgac
     4021 gccaattcgg ttcgatgatg cctgttggtg ccccttgggc ggcgagtgtg gccccgcagc
     4081 tccctctcga ccgaacctga ccgccccgag cttcgctcgc gcaactcctg gcaggtccat
     4141 ggccagcgga aaaggcccgt gggatgtacc cacgggccca gtcgacgatt tgggagcgct
     4201 cgctacgagc gtgcgtgccg ctccactgcg aagtgccaac cgctcaactg gcattgggag
     4261 ttcacgctgt tggccgtggc gaggcgcagc tgcgccttga ccgtgtacgt gccggggccg
     4321 acggcgagtg agcgctcgat ggcaccggat ccggcaactg actcggaaac gttggcctgg
     4381 cccatgaacg ggtaattgcc gttgggctcc atggtcgtgg cacctaccag gatccggacg
     4441 atgcagccgc tcgtctgaat cacggatcca tgactctgga agcgggccac gagcagcgcg
     4501 ttggtgcttg agggcacggt catcgagacc gtggcccccg gaatgtccga gtatgtggtt
     4561 gagttcgacg tggcgctggc cgtggtcgtg ttttcgcgaa cgaccttgat ctgcttctgc
     4621 gcgtttccgg tcacggccga tgtcgctgcg agaacgacgc cagtcgctgc caccatgatt
     4681 gcgagagcaa cgatgatcgc tgcgaagcgc gggatgtatc tggtcatatg agtatttctc
     4741 ctggtttgga tagggtttct gtgggcaatt gctggccttg gagaagtcac ttcatcggcg
     4801 acctctgtcg tcagcgacga tcgccgtaga gacacccagc gggctcagct ccgcgccacg
     4861 aagagccacc catacggcat cttctgactc cgccatatag acaaggaaca ggctgt
//
LOCUS       DATLCO010000136         2357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7537827, whole genome shotgun sequence.
ACCESSION   DATLCO010000136 DATLCO010000000
VERSION     DATLCO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7537827"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VMZ33_03355"
     CDS             complement(<1..876)
                     /locus_tag="VMZ33_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HUR16301.1"
                     /translation="MLIRALLAAIALLAIATGAVLAQMPAGPPYPEPQFDVVVYDYAN
                     VLSAATESQATATIVAIEKRVGAEVVVYTQIKPSADEVSTERDAKALLDQWLIGRAGF
                     FDGLVIFINMNRVPCIPGASGNGQVQLYAGDGYKGAYLSDGERQKIFDDEMVPHLRVC
                     DIDEAVLVALEKIDANATFDHRQTLETARRIDAAVGLVGGPLAFLLLVGAASWSWLRY
                     GKDPVYLDDASIHMPAPPPDLTAASGAVVWEGRATRRALTTAMLDLASRGELRFVPES
                     GLLSKKAGIQITNPDG"
     gene            complement(880..>2357)
                     /locus_tag="VMZ33_03360"
     CDS             complement(880..>2357)
                     /locus_tag="VMZ33_03360"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HUR16302.1"
                     /translation="RELLIGIEEAHALGDLPVIAQMTFGEELIAMDGTSPQTAATALA
                     RAGVDAVGVNCGVGPQICLDALVQMGRPSPGLARSIVPNAGLPQRVQGQFIYAAEPSY
                     FADEVQRFLDAGARIVGGCCGTSPDHIRHMRQALDIAHARERSLRGTGTGPPTEIGGT
                     PRTPVAERLTGTAAEPPPPTRLAAKLADNRFIVSVEIDPPRSVRIERTIEAARLLQEA
                     GVDIVNISDSAMARVRMGALAVAFGIQHDLDLECLVHFTTRDRNLMALESELLGAHAL
                     DVRNILALTGDPPRVGDYPTGTGIWDVDSVGLIGILSRLNRGEDQAGKPIGSPAGFTV
                     ACALDPTAQDLDHEITRLSAKIDAGAHVIMTQPIYGADQWDRFMDRAAMEWPGGLPRP
                     VLLGVLPLHTHRHAEFLHNEVPGITIPDEVRAAMAAAGERGAEVGLEMAHALLERLAP
                     QVQGTYIMPSFGRYELAAELARRIRADSRVAARLDASRSDA"
BASE COUNT          377 a          762 c          821 g          397 t
ORIGIN      
        1 tccatcgggg ttcgtgatct ggatgccggc tttcttgctg agcagtccgc tctccggcac
       61 gaacctcagc tcaccgcggc tggcgaggtc gagcatggct gtagtcaggg cccgcctggt
      121 cgctcggccc tcccaaacga ccgcgccaga tgcggcggtc aggtcgggcg gtggcgcggg
      181 catgtgaatg gacgcatcgt cgaggtagac cgggtccttg ccgtagcgca gccacgacca
      241 gctcgcggcg ccgacgagca gcaggaatgc cagcgggccg ccgaccagac cgaccgccgc
      301 gtcaatgcgg cgcgcagtct caagcgtctg ccggtggtcg aatgtggcgt tggcgtcgat
      361 cttctcgagg gccacgagga cagcctcgtc gatgtcgcac acgcgcaggt gcggaaccat
      421 ctcgtcgtcg aagattttct ggcgttctcc gtcggagagg tacgcgccct tgtagccgtc
      481 cccggcgtag agctgcacct gaccgttgcc cgacgcgcca ggaatgcacg ggacgcggtt
      541 catgttgata aagatcacga gtccgtcgaa gaacccagcc cggccgatca gccactggtc
      601 gagcagcgcc ttcgcgtcac gctcggtcga tacctcgtct gcgctcggct tgatctgggt
      661 gtagaccacg acctcggcac ccacgcgctt ctcgatggcg acgatcgtgg ccgtcgcctg
      721 cgactccgtc gcagcagaga ggacgttggc gtagtcgtag acgacgacat cgaactgggg
      781 ctccggatat ggcggtccgg ctggcatctg ggcaaggacg gcgccggtgg cgatggcgag
      841 aagggcgata gcggccagga gtgcgcggat cagcattcct tatgcgtccg atctcgatgc
      901 gtccaatctc gcggccacgc gggagtccgc acgtatgcgg cgcgccagct cggcggccag
      961 ctcgtaacgg ccgaagctgg gcatgatgta tgtgccctga acctggggcg cgaggcgctc
     1021 aaggagcgca tgagccatct ccaatccgac ctccgcgcct cgttcgccgg ccgctgccat
     1081 cgccgctcgc acctcatcgg ggatggtgat ccccggcact tcattgtgca gaaactcggc
     1141 gtggcggtgg gtatgcagcg gtagaacgcc gagcagcacc ggtcgcggca ggccaccggg
     1201 ccactccatc gccgctcggt ccataaatcg gtcccactgg tcggcgccat agatcggctg
     1261 ggtcatgatc acgtgggcgc cggcgtcgat cttggctgac agccgcgtga tctcgtgatc
     1321 gagatcctgg gccgtgggat caagcgcgca ggcgacggtg aagcccgccg gtgagccgat
     1381 cggcttgcct gcctggtcct cgccgcgatt gaggcggctg aggattccta tcagcccgac
     1441 cgaatcgaca tcccagatgc cggtccccgt cggatagtcg ccgacgcgtg gcggatcgcc
     1501 ggtcagcgcc aggatgttgc gcacatccag ggcatgagct ccgagcaact ccgactccag
     1561 cgccatgaga ttgcggtcgc gcgtcgtgaa atgaacaaga cactcgaggt cgaggtcgtg
     1621 ttgtatgccg aacgcgactg cgagcgcgcc catgcgcacg cgggccatgg ctgagtcgct
     1681 gatgttgacg atgtcgacgc cagcctcctg cagcagccga gcggcctcga tcgtgcgctc
     1741 gatccgcacc gaccgcggtg ggtcgatctc gacgctcacg atgaagcggt tatcggccag
     1801 ctttgcggcg agacgagtcg gtggcggtgg ctctgcggcc gttccggtca agcgctccgc
     1861 gactggagtg cgcggcgtac cgccgatctc ggtcgggggg cccgtgccgg ttccgcgcag
     1921 ggagcgctcg cgcgcgtggg cgatgtcgag cgcctggcgc atgtggcgga tgtgatcggg
     1981 gctcgtgcca cagcagccgc ccacgatacg ggcgccggca tccaggaagc gctgcacttc
     2041 gtcggcgaag tacgacggct cggcggcgta gatgaactga ccctggacgc gttgagggag
     2101 gccggcgtta gggacgatgc tgcgggccag acctgggctc ggccggccca tttgaacgag
     2161 ggcgtcgagg cagatctgtg gaccgactcc gcagttgacg ccgactgcat cgacaccggc
     2221 gcgcgccagg gcagtcgccg ctgtctgagg gctggtgccg tccatcgcga tgagctcctc
     2281 gccgaacgtc atctgggcga tgacgggcag atcacccagc gcgtgcgcct cctcgatgcc
     2341 gatcagcagc tcgcgca
//
LOCUS       DATLCO010000137         3607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6795310, whole genome shotgun sequence.
ACCESSION   DATLCO010000137 DATLCO010000000
VERSION     DATLCO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6795310"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..226)
                     /locus_tag="VMZ33_03365"
     CDS             complement(101..226)
                     /locus_tag="VMZ33_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16303.1"
                     /translation="MPNPRDGRSAGIVDEVPVKDALRAIEAAAREAASEARTQVH"
     gene            284..2092
                     /gene="lepA"
                     /locus_tag="VMZ33_03370"
     CDS             284..2092
                     /gene="lepA"
                     /locus_tag="VMZ33_03370"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HUR16304.1"
                     /translation="MIPQARLRNFSIIAHVDHGKSTLADRLLELTHTIEARQMTTQVL
                     DSMDLEREKGITIKARAVRLEYDARDGHRYGLNLIDTPGHVDFSYEVSRSLQACEGAI
                     LVVDAAQGIEAQTLANIHLALGQGLVIIPVINKIDLPSAQPEVVMEEMENILAIPREE
                     VLLVSAKDGTGVPEVLEQIVARIPPPKGDPARPLQGLIFDSHYDPYKGVVAYVRLVDG
                     RLREHEQIRLMASGAVSEILEIGVFRPQLVPAKELGPGEVGYVATGLKSVRDAQVGDT
                     LTSAARPASEPLPGYKPAKPLVFAGLYPVRGEDLPELRDALEKLHLNDGSISFEPETS
                     VALGTGFRCGFLGLLHMEIVQERLEREFDLDLIASAPSVEYRVQLLANRGEVVVDNPA
                     MLPPIGEIETISEPWVKLSVITPSQYIGTLMELSTGRRGTFETMEYIDADRVLLSFEL
                     PLAEVIVDYYDQLKSRSQGYASLDYEEIGYREGDLVRLDVLVAGEPVDALSLIAHRTR
                     AHSSGRVLVERLRQLIPRQMFEVPVQAAIGSNVIARETIRAMRKNVLAKCYGGDITRK
                     RKLLEKQKAGKARMKRVGSVEIPQEAFLAVLRTNEG"
     gene            2113..2895
                     /locus_tag="VMZ33_03375"
     CDS             2113..2895
                     /locus_tag="VMZ33_03375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HUR16305.1"
                     /translation="MQGTLEGLILLAVVGLLILLRFDARRFGAAEYDDEEAPGGWRTW
                     LRRLSWYVFGLLLLLVVYFLYPQPLSVLHLQMGEPRTQTLILGLGLGAMGIAFALVYA
                     WLRFSELRLPPARRYPGGVINAVATAFIDEAVFRGILLGLLIASDWPVNYAIAFQAVL
                     YALSTRLAGRGRPRGMLLLELAVGVIGGALTLQSGGIGAAFLGHALTRLAIFIAMGHA
                     GQMRAVAIDEEEEALEQTTSHAPAGWGVVVDREQGGPSDYRW"
     gene            2906..>3607
                     /locus_tag="VMZ33_03380"
     CDS             2906..>3607
                     /locus_tag="VMZ33_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUR16306.1"
                     /translation="MTSRHPVALYVHFPFCLSICPYCDFVVYAGRAAKGPDARIDRFV
                     QAVVREIGLRGVPGSALRSVYLGGGTPSLMSSQQVADVLQVADAAFGIADDAELTIEL
                     NPGPDERGQLAAFRAAGINRVSIGAQSLDPGELRRLGRRHSPADVATTVVAARSAGFD
                     NISLDLLYDVPGQTIAAWRDTLRGAIAMEPEHISAYALALDSYAPSPDHVPVSRGASA
                     WRARARGAQDQDRAAA"
BASE COUNT          584 a         1196 c         1163 g          664 t
ORIGIN      
        1 agtcgaacct cgaagatgtt cttacattcg cgccaagcgc gagatcttgt ttcctcgcac
       61 acgagcgtct ggcgctcagt gggcgcgacc gcggatggta tcagtgaacc tgcgtccgtg
      121 cctcagatgc cgcctcacgt gcagccgctt caatggctcg cagcgcgtcc ttgaccggca
      181 cctcgtccac gatcccagcg gaccgtccat cgcgtgggtt gggcatcgat cctgtcgggg
      241 ctgggtcgga tgtaagggcg gcccactata gactcgggcc caggtgatcc cccaggcgcg
      301 tctgcgcaac ttttccatca ttgcccatgt cgatcacggc aaatcgactc ttgccgaccg
      361 gcttctcgag ctgacgcaca ccattgaagc ccggcagatg acgacccagg tcctcgactc
      421 catggatctc gaacgcgaga agggcatcac catcaaggcg cgcgcggtgc ggctcgaata
      481 cgacgcacga gacggtcatc gctacggcct gaacctgatc gacacgcctg gccacgtcga
      541 cttttcgtac gaagttagcc ggtccctcca ggcatgcgag ggcgcgatac tcgtggtcga
      601 cgccgcgcag gggatcgagg cgcagacgct ggcaaacatc catctcgcgc tgggccaggg
      661 cctggtgatc atcccggtca tcaacaagat cgacctgccg tccgcccagc ctgaagtcgt
      721 gatggaggag atggagaaca tcctcgccat cccgcgtgag gaggtgctgc tggtatcagc
      781 caaggacggc accggcgtgc ctgaggttct cgagcagatc gtggcgcgga tccctccccc
      841 aaaaggcgat ccagcgaggc cgctccaagg gctgatcttc gattcgcact acgaccccta
      901 caagggcgtg gtcgcctacg tccggctggt cgatggccgg ctgcgggaac acgagcagat
      961 caggttgatg gcctctggcg cggttagcga gatcctggag atcggcgtct tccgaccgca
     1021 gctcgtaccc gccaaggagc tcggtccggg cgaggtcggc tacgttgcca cgggcctcaa
     1081 gagcgtgcgc gatgcacagg tcggcgacac gctcacctct gccgcgcggc cggcgtccga
     1141 gccactcccc ggttacaagc cggcaaagcc actggtgttc gcgggcctct accccgttcg
     1201 tggcgaggac ctgccggagc tacgcgatgc actcgagaag ctccatctca acgacggctc
     1261 gatcagcttc gagccggaga cgtcggtcgc tctcggaacg gggtttcgct gcggcttcct
     1321 gggccttctg cacatggaga tcgtccagga gcgcctggag cgggagttcg acctcgatct
     1381 catcgcgtcc gcgccatcgg tcgagtaccg cgtccagctg ttggccaatc gtggcgaggt
     1441 cgtcgtcgac aacccggcaa tgttgccgcc gatcggcgag atcgagacca tctctgagcc
     1501 gtgggtcaag ttgagcgtga tcacaccgtc gcagtacatc ggcacgctca tggagctctc
     1561 caccggccgc cgcggcacgt tcgagacgat ggaatacatc gacgccgatc gcgtgctgct
     1621 ctcctttgag ctgcctctgg ccgaggtgat cgttgattac tacgatcagc tgaaaagccg
     1681 tagccagggg tacgcgtcgc tcgactatga ggagatcggc taccgcgagg gggatctggt
     1741 gcgcctcgac gtgctcgtcg cgggtgagcc ggttgacgcc ctgtcgctca tcgctcaccg
     1801 cacgcgtgcg cattcgtccg gccgcgtcct cgtcgagcgg ctgcgccagc tgatcccgcg
     1861 tcagatgttc gaggtaccgg tccaggccgc gatcggctcc aacgtcatcg cccgcgaaac
     1921 gattcgcgcc atgcgcaaga acgtcctggc caagtgctac ggcggtgaca tcacgcgcaa
     1981 gcgcaagctg ctcgagaagc agaaggccgg caaggcgcga atgaaacgcg tcgggtcggt
     2041 cgagataccg caggaagcct tcctggctgt gcttcggacg aacgagggtt agtcggtatg
     2101 ggccaccgtt agatgcaggg cacgctcgag ggcctgatcc ttctggccgt tgttggtctg
     2161 ctgatcctgc tgcgctttga tgcccggcga ttcggcgccg ccgaatatga cgacgaagaa
     2221 gcaccgggcg gctggcggac atggttgcgt cggctctcct ggtacgtgtt cggcctgcta
     2281 ctgctgctcg tcgtctactt cctctaccca cagccgctgt cagtgctgca tctacagatg
     2341 ggcgagccgc gcacgcagac gctgatcctc ggtttgggtc tgggggcgat gggcatcgcc
     2401 tttgccctgg tctacgcctg gctgcggttc agcgagctgc ggctgccacc ggcgcgtcgc
     2461 tatccaggcg gagtcatcaa cgcagttgcc acggcgttca tcgacgaggc cgtcttccgc
     2521 ggcatcctgc tcggactact gatcgcgtcg gattggccgg tcaactacgc gatcgcattt
     2581 caggccgtgc tgtatgcgct atccacccgg cttgccggtc gcggacgacc gcgcgggatg
     2641 ctgctcctcg aactggccgt cggcgtgatc ggcggcgccc tcacgctgca atcgggcggc
     2701 atcggagcgg ccttcctggg tcatgcgctg actcgcctgg ccatattcat tgccatggga
     2761 cacgctggac agatgcgggc cgtcgcgatc gacgaagagg aggaggccct cgagcagacg
     2821 acctcccatg cgcccgccgg gtggggtgta gtcgtcgacc gcgagcaggg tggcccaagc
     2881 gattaccgct ggtagcgctg ctcccgtgac ttcgcggcac ccggtggccc tgtacgtcca
     2941 cttcccgttc tgtctttcga tctgtcccta ctgcgacttc gtcgtctacg cgggtcgcgc
     3001 cgccaagggg ccggacgcac gcatcgaccg tttcgtgcag gctgtcgtcc gtgagatcgg
     3061 gctgcgcggc gtccccggat ccgcgttgcg aagcgtctat ctgggcggtg ggacgccgtc
     3121 actgatgtcg tctcagcagg tggccgacgt tctccaggta gcggatgctg cctttgggat
     3181 cgccgacgac gctgagctga caattgagct gaaccccggc cctgacgagc gcggccaact
     3241 cgcagccttt cgcgcggcag gcatcaaccg cgtcagcatc ggcgcgcaga gcctggatcc
     3301 tggtgagctt cgccgtctcg gacggcgcca ctccccggcc gacgtggcca cgaccgtcgt
     3361 cgcggcgcgg tcggccggct tcgacaacat cagcctcgac ctgctctatg acgtgcccgg
     3421 ccagaccatc gcagcgtggc gtgacacctt gcgtggcgcg atcgccatgg agcccgaaca
     3481 catctcggca tacgcactgg ccctggattc gtatgcgccc agccccgacc acgtacccgt
     3541 ctcgcgcggt gcctccgcct ggcgtgcgcg agcgcgcggc gcacaggacc aggaccgtgc
     3601 cgcagcc
//
LOCUS       DATLCO010000138        11847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_919942, whole genome shotgun sequence.
ACCESSION   DATLCO010000138 DATLCO010000000
VERSION     DATLCO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919942"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2508)
                     /locus_tag="VMZ33_03385"
     CDS             complement(<1..2508)
                     /locus_tag="VMZ33_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HUR16307.1"
                     /translation="MPTSFAELHCHSNFSFLDGASPVDDLAERAATLGMTALAVTDHN
                     GLYGAVRFATAVHEAGLRAIVGMEVELLDSVVPDLSGVVVPRRQRRKSRAKAGGDLEL
                     DLSDPPPAEGRAVRPAIDRLRPPGHREPKREDLRGVRSRELGPHLVLLARDMLGYRSL
                     CRLTSSAHLAGTKGAPKFTHALLAKHTEGLVALSGCRHGEVARRLVAGDRNGAAEAAG
                     RLAAMFASEGSSPRSGAGAGAGFFLEMQHHLLPDDDFLVTETVRLANQTGLPTVVTNG
                     AHYAYPADRELQDVLVCIKHGKTLEESAHLRRPNGEFHLKSLDEMAALPPATGALGPK
                     VAAAWASGLSSAGELAESCDVDFDFEQYRFPGFPVPVEETAFSYLERLCHDGMRKRYI
                     PLRPAVVKQLAHELEVIERTGLAEFFLICWDIMRFCRERGIPAQGRGSAGDSIVAYVL
                     GITRVDPIRHRLLFERFINEGRTSYPDVDIDFASSRREEVIQYCYERYGADHTGMVCN
                     LVTYRARSAVREVGYALGFPRPLVDRVAKALETYDSVMVRRDLEAEGGFGEFFTPDSA
                     SDAVAASPAAGGSLSAAVSDVLAGERGLLDHMGQLKRPARMLQALSDDEGGPGDSPAS
                     VRWLRAEMERAADERLIEAETHPALQRPPRRVDPDAGEDYRPPALRAADNEGSRGRPI
                     AALSAPLLRQSAAVRLDDAGSSEQEAVDNLHAQKTEASKSLRPESADSTPLPPVRSSD
                     TDKHASGARAGLQAVTGAAVGAAVGAVTGAAAHPSTIGLTPWDRWLELTSRIDGFPRH
                     LSIHVGGMLITAAPLIDIAPLERATMPGRVVVQYDKRD"
     gene            2584..3492
                     /locus_tag="VMZ33_03390"
     CDS             2584..3492
                     /locus_tag="VMZ33_03390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUR16308.1"
                     /translation="MPASRPPLFALAAVPVLALVAIVVVLLLAPGSRPTDIAALLSAS
                     PEPTPTLNGSSTDGATTPPGEAPTDPPTGQPTDGPGSTPDASGGIPPKDGGAGSVAPE
                     DLEGYIWPVRNAFVTSRFQQRDLGFVVIDGEEYHDGLDLASSCGDRVRAAHSGTVLYA
                     GRNFDPYIGYKGDASRIYARLERLGRTNEQPIVVVIDDGNSYRSIYVHLSRSLVEQGA
                     IVEAGDFIGVEGMTGLATGCHLHYGLIRMDGEWQQVLPRLTRFGYPPLVRERVDPLDV
                     LDWTDEFAPEKLRNRVLGTPSPAPQP"
     gene            3520..3708
                     /locus_tag="VMZ33_03395"
     CDS             3520..3708
                     /locus_tag="VMZ33_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16309.1"
                     /translation="MSTLPAKPFTRHTVQSAPVTRSMTPLRAAAPLVRALVLASIVTA
                     LIMIGLPAVLAIARASTL"
     gene            complement(4131..4379)
                     /locus_tag="VMZ33_03400"
     CDS             complement(4131..4379)
                     /locus_tag="VMZ33_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16310.1"
                     /translation="MTRLLGHHPLIDVDLDRVGMPCRLRWEGGSEAVEVCNQWRIDEA
                     WWRRPVSRDYYKLVGERLLALVYRDRVDGTWHLERVYD"
     gene            complement(4376..5656)
                     /locus_tag="VMZ33_03405"
     CDS             complement(4376..5656)
                     /locus_tag="VMZ33_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HUR16311.1"
                     /translation="MRFLYLDWPHLCHRLELARARQAPLDELVVIGGQPWEAGTVLDC
                     SPSARRLGVLRGQPLGTAHRLVPEAQFLPADHDAYRGRMDAALEEMARFTPSVESDSD
                     PEARTFGQILLGIEGLHRLWGDEPRLTSRIVAAVRAHLPDEPRLGIGNTRFGAQVAAV
                     VGRGTLESIPIGDAAAEAEAEAAYLAPLSITLLPANDETRERLRVFGLTRIGEFAALA
                     RSSVIARFGEHGGMLHDLARGMDGRVLRPRRAIERLAAEAELEPPVDSVEPLRFVLRN
                     LCGALCEQLNARGAGATRAVLVLELERGLPLVLEQVLPEPAASPDLLERLLVARLIAE
                     SPRIAVTKLSVELRGEQVVSGQQLGLFAPQLARAARLDWQLTGLALRFGVDRLLRTRI
                     LDPEATLPELRVEWTPATTDPLSLAAASVSPRAP"
     gene            complement(5715..6641)
                     /locus_tag="VMZ33_03410"
     CDS             complement(5715..6641)
                     /locus_tag="VMZ33_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16312.1"
                     /translation="MVMESVPAPLTTALARLESRWGSAAVRLGTTNIASRAAAPQASA
                     EGNLHVDGSVAPGPDHIVDGALALAASPQPAPELGPSLSDDIVPTGFARLDELLGTGG
                     LPRGVSASFRGARSSGKTALALHCLAQAQARGGIVAFLDLCRIFDPLEAAARGVDLRW
                     LLVLRPADADEGWALAAALVSGHQIDLLVIDLPQKLRGGHDAALRRLAAHARRSTVRM
                     IVLEPMGLAASVHGALAESVAVRLELEQRSWLRAGRDVVGRCVAVNVAKNRFGPPGRE
                     TEIEIHYLADGDHGAALDRYSARERPAIRRVA"
     gene            6695..7312
                     /locus_tag="VMZ33_03415"
     CDS             6695..7312
                     /locus_tag="VMZ33_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16313.1"
                     /translation="MTDEDTEFYRWYGPWKPLDPNGVARVLEGTSFRWWIIGGWAIEA
                     FTGKPREHEDIDVSFFRDDLPQVLDHLNRRYCVWSNASGTLRPLKQAADLLPDCRQLW
                     VRPDGGEPWVMDLAMNPHDGDTWISVRDDAIRMPIDDATFTAHGISYLRPEIVMAMKA
                     RWARPKDDADLAEILPRLDQTRLAWLRATIDQLHPGHRWLETIAA"
     gene            7309..8061
                     /locus_tag="VMZ33_03420"
     CDS             7309..8061
                     /locus_tag="VMZ33_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16314.1"
                     /translation="MNSVRPAFDRLTSRQRLLVLLIGLVAVAILLLLVVQCTQGSPSG
                     SPPPTNSGRQTTAARTDGATGKPTDGSTDQPSGDPTGSPEPTEPTITPPPDLDAPTAE
                     LFQHIPNALRSSCQTGPFLAPVVATLYCTTEDGAIAVQYLTYPDADSMNAAHGTSVAL
                     AEAEPGTGRCYNPAGDGTFSATLGRWPAEQPYSVGGQEVGRYLCFESNQPTIAWTDER
                     FNILTLASGPADTLDRLVTFWVLRSGPYPLLP"
     gene            8177..8252
                     /locus_tag="VMZ33_03425"
     tRNA            8177..8252
                     /locus_tag="VMZ33_03425"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8210..8212,aa:Ala,seq:cgc)
     gene            complement(8322..8897)
                     /locus_tag="VMZ33_03430"
     CDS             complement(8322..8897)
                     /locus_tag="VMZ33_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16315.1"
                     /translation="MTELEIHAYAGDCLVDAHLELPDGVRLTDYLNESTEVPLTQVRM
                     TALDDGRIVEAENLLVSTDEIHAVEAKDRGGQPGRRIRTRSSEVNVVVGPYRVQGYLH
                     GPTAGDPLGALSRRQAMVPMTSAKIGFLVAGELRVKDCAALIVNRLHANIEKPEGDAP
                     SILDQIGLLPVDPNAKDLTGELLIRRPPSDE"
     gene            complement(9107..9829)
                     /locus_tag="VMZ33_03435"
     CDS             complement(9107..9829)
                     /locus_tag="VMZ33_03435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HUR16316.1"
                     /translation="MAAALLDLPAYTIRRSARARHSRITITEQAQAVVVLPLRAPEQD
                     AADLVARYRTWVHRNVGRMAARRAALDGRPSIDSGRELFWLGIRHRVVSLAAIDGRAR
                     SSVRVVDGRVVVTSALQEKRSTAQIIDAWFRSHAKREISARVAARAPDLGVSPRSVTI
                     RDQKSRWGSASARGTLSFSWRLLMCPPEVLEYVVVHELAHMKITGHPRAFWRLVGRHH
                     PDVRGARRWLREHHDEIRHALD"
     gene            9864..9939
                     /locus_tag="VMZ33_03440"
     tRNA            9864..9939
                     /locus_tag="VMZ33_03440"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9897..9899,aa:Ala,seq:ggc)
     gene            10151..11581
                     /locus_tag="VMZ33_03445"
     CDS             10151..11581
                     /locus_tag="VMZ33_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16317.1"
                     /translation="MASTLTFQQDDRLATHGTLTPETGGTLEVTAADGTRFRLDVPPG
                     AVWEETEASVTPLIDVHGVGDGPVHAVRLEPEGITLFEEARLLVLPATPIAVAEQLIF
                     HARGDGSNALAALVDPEASDVVILLQHFSIAGTAKAAAAASWNIGQSHSAVERLSNEV
                     GKYVQEQRKRAARGDDGLSEVSEVLERLLRDAERDVIAPLREAALLTCEGATELMKML
                     RAVERQRALVGISTEATFGAMLAEVNRIWTSSFAICEREAVVKCQVHPDPGLLVEFWI
                     TWDRSRSLAGQTHMSPDGDLAKLARDKCGWSYSFNRTLEMAQGQMSVRYEWTGIVRVA
                     DEGVISGTGTGFLRGSQGTCTYGDPPTEVPIDVTSSATFAFEFQGRRAGDTLTIEHPA
                     ITADVVVEGSFAPCMEAGPLAEFVIETALRDPALLPPAVVADRVGRTTERRNGATSVT
                     TLSPPPTPRPTPSPSSQPPVVGPSTP"
BASE COUNT         2046 a         4108 c         3804 g         1889 t
ORIGIN      
        1 gtcgcgtttg tcgtattgca ccacgacacg acccggcatc gtggctcttt caagcggcgc
       61 aatgtcaatc aggggcgccg cagtaatgag catcccacca acgtggatcg acagatgtcg
      121 cgggaatcca tcgatgcgcg aggtcagttc cagccaccgg tcccacgggg tcagcccaat
      181 cgtcgacgga tgcgccgccg ccccggtcac cgccccgacc gccgccccga ccgccgcccc
      241 ggtcaccgcc tggagaccag cccgggcacc cgaagcgtgt ttatcagtgt cactgctacg
      301 aactggagga agtggcgtgc tgtccgctga ttctggccgg agtgacttcg acgcctccgt
      361 cttttgagcg tggaggttgt cgacggcttc ctgctccgac gatccagcgt cgtctagccg
      421 gacagccgcg ctctgtcgta acagtggtgc tgataacgcg gcaatcggcc ggccgcgtga
      481 cccctcgttg tccgcggcac ggagcgccgg agggcggtag tcctcgcccg catcggggtc
      541 tacacgacgc ggcggtcgtt gcagcgcggg atgtgtctcg gcctcgatca ggcgctcgtc
      601 agcggccctc tccatctcgg cccgcagcca acgcaccgac gctggcgagt cgcccggccc
      661 accctcatcg tcggagagcg cctggagcat ccgtgccggt cgcttgagct ggcccatgtg
      721 atcgagcagg ccccgctccc cggcgagcac gtccgacacg gcggcggaca acgagccgcc
      781 tgccgctggc gacgcagcca cggcgtccga cgcggaatcc ggcgtgaaga actcgccgaa
      841 gccgccctcc gcctccaggt cgcgccggac catcaccgag tcgtaggttt ccaaagcctt
      901 cgccacgcga tcgacgagcg gccgcgggaa ccccagcgcg tacccgacct cacgcaccgc
      961 cgatcgcgcc cggtacgtga ccaggttgca caccatgccc gtgtggtcgg ccccgtagcg
     1021 ctcgtagcag tactggatga cctcctcgcg ccgcgacgag gcaaagtcga tgtcgacgtc
     1081 cggatacgaa gtgcgtccct cgttgatgaa gcgctcgaac aaaagcctgt gccggatcgg
     1141 gtcgacgcgc gtgatcccaa gcacgtaggc cacgatcgag tcacccgcgg aacctcgccc
     1201 ctgggccggg atcccgcgct cgcggcagaa ccgcatgatg tcccagcaga tcaggaagaa
     1261 ctcggccaga ccggtccgct cgatgacctc cagctcgtgc gccaactgct tcacaacggc
     1321 agggcgaagc gggatgtagc gcttgcgcat cccgtcgtgg cacagccgct ccaggtacga
     1381 aaacgccgtc tcctcgaccg gcaccggaaa acccgggaag cggtattgct cgaagtcgaa
     1441 gtcgacgtcg cagctctcgg ccaattcacc tgccgacgac aggcccgacg cccacgcggc
     1501 agccactttc ggtcccagcg ccccagtcgc cggcggcagc gcagccatct cgtcgagcga
     1561 cttcaggtgg aactcgccgt tggggcgtcg cagatgggcc gactcctcga gcgtcttgcc
     1621 gtgcttgatg cacaccagca cgtcctggag ctcgcggtcg gccgggtagg cgtagtgagc
     1681 gccgttcgtg accaccgtcg gcagcccggt ctggttcgcc aaccgcaccg tttccgtgac
     1741 aagaaagtcg tcgtcgggca gaaggtggtg ctgcatctcg agaaagaagc ccgcgcccgc
     1801 gcccgccccc gacctcggac tcgagccctc gctggcgaac atcgcggcca gtcgaccggc
     1861 cgcttcggcc gccccattcc ggtcgcccgc aaccagccgc cgcgccacct cgccgtgccg
     1921 gcaaccggac agggcgacca agccctcggt gtgcttcgcc agcagcgcat gcgtgaactt
     1981 cggcgcgccc ttcgtcccgg ccaggtgggc gctgctcgtg agccggcaca ggctccggta
     2041 ccccagcatg tcgcgcgcca gcaggaccag gtggggcccc agctcacgcg atcgaacgcc
     2101 acgcagatcc tcacgcttcg gctcccgatg accgggcggg cgcaggcggt cgatagccgg
     2161 tcggactgcc cgaccttcag caggtggcgg gtccgataaa tcgagctcca ggtcgccgcc
     2221 cgccttggcc cgactcttcc gtcgctgacg gcgcgggacg acgacgcccg acagatccgg
     2281 cacgaccgag tcaagaagct ccacttccat gccgacgatg gcccggaggc cagcctcatg
     2341 aacggcagtt gcaaagcgga cggcgccgta tagaccgttg tggtctgtca cggcgagcgc
     2401 ggtcatgccc agcgtggccg ctcgttcggc gaggtcgtct acaggcgagg caccgtcaag
     2461 aaacgagaag ttggagtggc aatggagctc ggcgaagctc gtgggcactc atcgataata
     2521 gaacacctgt tctagttcca aacccgctga cgcgagtggc tcgatcatgg tttgattagg
     2581 acgatgccag cgtcccgtcc gccccttttc gcgctggccg ccgtcccggt tctggcactc
     2641 gtcgcgatcg tcgtcgtgct gctcctggca ccgggctccc gaccgaccga tattgcagcg
     2701 cttttgagcg cgtcgcccga gccgacacca accctgaacg ggagttcgac cgacggcgcg
     2761 accacgccgc ccggcgaggc accgaccgac ccacccaccg ggcagcctac tgatggcccc
     2821 gggtcaacac ccgacgcgag cggtggcatt ccccccaagg acggcggcgc gggcagcgtc
     2881 gcgcccgaag atctcgaggg ctatatctgg ccagtccgca acgcgttcgt gacaagccgc
     2941 ttccagcagc gtgatttggg cttcgtggtg atcgatggcg aggagtacca cgacggtctg
     3001 gaccttgcct cgagctgcgg cgatcgcgtc cgggcggcgc actccggcac tgttctgtat
     3061 gccggacgca acttcgatcc gtacatcggc tacaagggtg acgcgtcgcg tatatacgct
     3121 cggttagagc ggctgggccg caccaacgaa cagcccatcg tcgtcgtgat cgacgacggg
     3181 aatagctatc gcagcatcta cgtccatctg agtcgatctt tggtggaaca gggagcgatc
     3241 gttgaggccg gggacttcat cggtgtcgaa ggcatgactg gtctcgcgac cggctgccac
     3301 ctgcactacg gcctcatcag gatggacggg gagtggcagc aagtcctgcc gcgactgacc
     3361 cgttttggat atccgccgct ggtccgggaa cgcgtcgatc cgctcgacgt cctcgactgg
     3421 accgacgaat tcgcccccga aaagctgcgc aaccgggttc ttggtacgcc ctctcccgcc
     3481 cctcaaccct gaactgaaca ccggagccct acaaaaatca tgtcgacttt gcccgccaag
     3541 cccttcaccc gtcacaccgt ccaatccgca cccgtcacac gctcgatgac gcctttgcgt
     3601 gctgccgcgc cccttgtccg cgccctggtc ctggcgagca tcgtgacagc gctgatcatg
     3661 atcggcctgc ccgccgttct cgctatcgcc cgcgcctcga ctctgtaggg gtccctgcgg
     3721 gtgtcgtgcc cgccacgccc gtatcctgtc gccgtgcgtc cacaggtatt cgagatgatt
     3781 gccgccgccc tcctctttct ggtgctggcc ggccttgtcg tcgtgatcgt cgtgtcgacc
     3841 attccgcctg acggagtgcc gacgcttcag tagtcgctgc gcggtctggc ccgctcagcc
     3901 gcgcacaccc gcgtcgtcgc gacacgtgga atccgcagac tcattgcatt ttcagacggc
     3961 cgcgctggca cgtcacacgc ccctgcgcgg ttgcgtccca acgttaagca agtagcaatc
     4021 cgcgcggtcg ctggggcggt ccgaacctct cctcctcgtc ggaccacgaa acgacccgtt
     4081 cccgcactcg gccccggtct cccccggggt cgagttcttt tttggggact tcagtcgtag
     4141 acgcgctcga ggtgccacgt cccgtcgacg cggtcgcgat agaccagcgc caggagccgc
     4201 tcgccgacga gcttgtagta atcacgactg accggccgac gccaccacgc ctcgtcgatg
     4261 cgccattggt tgcagacctc gacggcctca ctgccgccct cccagcgcag acggcagggc
     4321 atgccgacac gatccagatc gacgtcgatc agcgggtgat ggccgagcaa ccgcgtcatg
     4381 gcgcacgtgg cgagacggac gcggccgcga gggacagcgg gtcggtggtg gccggcgtcc
     4441 actccacacg cagctcaggc agcgttgcct cagggtcaag gatgcgtgtg cgcagcagac
     4501 ggtcgactcc gaaacgcagc gccaggccgg tcagctgcca gtcgagccgc gcggcacggg
     4561 cgagctgcgg cgcgaatagc ccgagctgct gaccggacac gacctgctca ccgcggagtt
     4621 caacggaaag cttcgtgact gcaatccgcg gcgattcggc gatgagtcgc gcgacgagca
     4681 gtcgctccag gagatccggg gacgcggccg gttcaggcag cacctgctcc aggacgagtg
     4741 gcaggccacg ctccagttcc aggacgagca cggcgcgcgt cgcgccggcg ccgcgagcgt
     4801 tgagctgctc gcacagcgcg ccacacaggt tgcgcaacac gaagcgcagc ggctcgaccg
     4861 agtcaaccgg cggctcgagc tctgcctcag ccgcgagacg ctcgatcgcg cgccgcggtc
     4921 gcaacacccg tccgtccatg ccgcgtgcaa ggtcgtgcag catcccgccg tgctcgccga
     4981 agcgcgcgat caccgacgac ctggcaagcg cggcgaactc gccgatccgc gtcaggccga
     5041 agacgcgcag ccgctcgcgc gtctcgtcgt tggcagggag cagcgtgatc gacagcggcg
     5101 cgagatacgc cgcctccgcc tccgcctccg ctgccgcgtc gccgatgggg atgctttcca
     5161 acgtgccgcg accgacgacg gccgcgacct gcgcgccgaa ccgtgtgttg cctatcccga
     5221 gccgcggctc atccgggaga tgggcacgga ccgcggccac gatccgcgat gtcaggcgcg
     5281 gctcgtcacc ccataaccgg tggaggcctt cgatcccgag caggatctgc ccgaacgtcc
     5341 gtgcctcggg atcgctgtcc gactcaacgg acggcgtgaa cctggccatc tcttcgagcg
     5401 cggcgtccat gcgcccgcgg tatgcgtcgt gatcggcggg gaggaactgc gcctcaggga
     5461 cgaggcgatg cgccgtgccc aatggctggc cgcggaggac gccgagccga cgcgccgagg
     5521 ggctgcaatc aaggaccgtg cccgcctccc atggctgccc gccgatgacg acgagctcgt
     5581 cgagcggcgc ctggcgggca cgggccagct caaggcggtg gcaaaggtgt ggccaatcga
     5641 gataaaggaa acgcatcggg cacgtagcgt ttggagggtt ggattagggg tcggtcactc
     5701 ggtcgatcgg gacctcaggc aacgcggcgg atggctggtc gttcgcgtgc ggagtaccgg
     5761 tcaagggcgg caccgtggtc accatcggcg aggtagtgga tctcaatctc cgtttcgcgg
     5821 ccgggtggcc cgaaacggtt cttggcgacg ttcacggcca cgcaacgacc gacgacgtcg
     5881 cgtccggcgc ggagccacga acgctgctcc agctccaggc gtacggccac ggattcggcg
     5941 agggcgccgt ggaccgatgc agccaggccc atgggctcga ggacgatcat ccgcaccgtg
     6001 gaccggcgcg catgcgcggc caaccgacgt agcgcggcat cgtgtccgcc gcgcagtttt
     6061 tggggcagat cgatcacgag caggtctatc tgatgtccgg acacgagtgc ggcggcgagt
     6121 gcccagccct cgtccgcatc cgcgggccgc agcacgagga gccagcgcag gtccacgccg
     6181 cgtgccgccg cctcaagcgg atcaaagatc cggcacagat ccaggaacgc gacgatcccg
     6241 ccccgcgcct gtgcctgggc caggcaatgc agcgccagtg cggtcttgcc actggaacga
     6301 gcaccacgaa aagaggcact gacgccacgc ggcaagccgc cggtgccgag cagctcgtcc
     6361 agccgggcga atccggtggg gacgatgtca tccgacagcg atgggccgag ctccggcgct
     6421 ggctggggag aggccgccag cgccagagca ccgtccacaa tgtggtctgg cccgggcgca
     6481 accgaaccat ccacgtgtag gtttccctcc gcagacgcct gcggcgctgc tgcgcgcgaa
     6541 gcaatatttg ttgttccgag acgcaccgcg gcgcttcccc aacgcgattc aagccgcgcc
     6601 agcgcggtgg tcagaggagc gggcacgctc tccatcacca tgtggcgatc atagaacaaa
     6661 tgttctaatc gtcaacccct tacgctggag tcagatgacc gacgaagaca ccgagttcta
     6721 ccgctggtac ggaccctgga aaccgctcga cccaaatggg gttgcacgtg tgctggaggg
     6781 gacgagcttc cgctggtgga tcatcggtgg ctgggcgatc gaggcattca ccggaaaacc
     6841 acgcgaacat gaggacatcg acgtcagctt ctttcgcgac gatctcccgc aggtgctcga
     6901 ccacctgaat cgccgctact gcgtctggtc caacgcgtcc ggcaccttgc ggccgctcaa
     6961 gcaggccgcg gacctcctgc ccgattgccg ccagctgtgg gtacggcctg acggcggtga
     7021 gccgtgggtc atggacctgg ccatgaaccc gcacgacgga gacacctgga tctccgtccg
     7081 cgacgacgcc atccgcatgc ccatcgacga cgcgaccttc accgcccacg gcatcagcta
     7141 cctccgaccg gagatcgtca tggccatgaa agcgcgctgg gcacggccga aggacgacgc
     7201 cgacctcgcc gaaatcctgc cccggctcga tcagacccgc ctggcctggc tgcgtgccac
     7261 gatcgatcag ctccatcccg gccatcgctg gcttgagacc atcgccgcgt gaatagcgtg
     7321 cgtcccgcgt tcgatcggct caccagccgg cagcgcctgc tcgtcctgct catcggcctc
     7381 gtcgccgtcg caatcctgtt gctcctcgtg gtccagtgca cgcagggctc gcccagcggc
     7441 tcgccaccgc ccacaaacag cggcaggcag acaaccgcag cccgaactga cggcgccacc
     7501 ggcaaaccaa ccgacgggtc gaccgatcag ccatcaggcg atccgaccgg gagcccggag
     7561 ccgaccgagc caaccatcac tccgccaccc gacctcgacg cgccgacggc cgagctgttc
     7621 cagcacatcc ccaatgcgct gcgctcgtcc tgccagaccg gaccgttcct cgcgccagtg
     7681 gttgcgaccc tctactgcac gaccgaggac ggggcgatcg ccgtccagta cctgacctac
     7741 cccgacgccg actcgatgaa cgccgcgcac ggcaccagcg tcgcgttggc ggaggctgag
     7801 cccgggacgg gccgctgcta caacccggcc ggcgacggga ctttcagtgc aacgctgggc
     7861 cggtggccgg cagaacagcc gtactcggtt ggcgggcagg aggtcgggcg ctacctctgt
     7921 ttcgagtcaa accagccgac cattgcctgg accgacgagc ggttcaacat cctgacgctc
     7981 gccagcgggc cggccgacac gctcgatcgc ctggtcacgt tctgggttct tcgctccggc
     8041 ccctaccccc tgctgccatg actcagctgg tggacgaaat cgctaacccc agggctggga
     8101 tgactgctca caccggtcgg cttacccatc ggctgcgggc ccgtggaagg cccgtcggta
     8161 tacttgccac tcccgtgggg atgtagctca gctgggagag cactgcgttc gcatcgcagg
     8221 ggtcaggggt tcgagtcccc tcatctccac catctcctgg cgcgaactca ggatctgacg
     8281 tttgcccgtc gcctcttgac cgacagccgc tccagcggta ctcattcgtc gctgggcggc
     8341 cgccgtatca agagttcgcc cgtgaggtcc ttggcattag gatcgacagg caggagaccg
     8401 atctggtcaa gaatgctcgg cgcgtccccc tccggcttct cgatattcgc gtgcaggcgg
     8461 ttgacgatga gcgccgcgca gtccttcacg cgcagctcgc cagccacgag gaacccgatc
     8521 ttggcgctgg tcatcggcac cattgcctga cggcgcgaaa gggcccccag cgggtcgccg
     8581 gctgtcggcc cgtggaggta tccctgcacc cgatacggcc caacaactac gtttacttct
     8641 gatgaccggg ttcggatgcg acgtccgggt tgcccgcccc tgtccttagc ctcgaccgcg
     8701 tggatctcgt ccgtcgagac cagcaggttc tctgcttcga caatccggcc atcgtcgagt
     8761 gcggtcattc gaacctgcgt cagcggaacc tctgtcgatt cgttcaggta atcggtcagc
     8821 cgtaccccgt cgggcaattc gagatgggca tcgacgagac aatcaccggc gtaagcgtgg
     8881 atttcgagct cggtcatgcg gcctccaagg tggctcgcgc ccttaaataa gcgcgagcat
     8941 cgagcgtctt tccacggctg ggtccgtgca tcgggacgcc aacgccaccc aagcacttcg
     9001 ggtgaatggt ggcagaaatg ccggtcgccg gtgcgatcct ccggtcacgc caatcgccga
     9061 cccgtggctt cttcctcctt gcagcggcgc gactcgacgc gtggcgtcag tcgagcgcgt
     9121 gcctgatctc gtcgtgatgc tccctcagcc agcgccgcgc gccacgaacg tcggggtgat
     9181 gacggccgac aagccgccag aacgctcgcg gatggccggt gatcttcatg tgcgcaagct
     9241 cgtgcacgac gacgtactcg agcacctcag gcgggcacat cagcaaccgc cacgagaagg
     9301 acagtgtgcc gcgtgccgaa gcgctgcccc agcgcgactt ctggtcacgg atggtgacgc
     9361 tcctgggcga gaccccgagg tctggcgccc gagccgcgac ccgagcactg atctcgcgct
     9421 tggcatgtga ccggaaccag gcgtcgatga tctgcgctgt cgagcgcttt tcctggagcg
     9481 ccgaggtcac cacgacccgg ccgtcaacta cgcgcaccga cgagcgagca cgaccatcga
     9541 ttgcggcgag ggacaccacg cgatggcgga taccgagcca gaagagctcc ctgcccgagt
     9601 cgatcgacgg gcggccatca agcgcggcac gccgggccgc catccgtccc acatttcgat
     9661 gcacccacgt ccgatatcga gcgaccaggt ctgccgcgtc ctgctccggc gcgcgcagag
     9721 ggagcacgac gacggcctga gcctgctcgg tgatcgtgat tcgcgagtgt cgcgctcgcg
     9781 ctgagcgcct gatcgtgtat gccggaaggt cgagcagcgc cgcggccata cgaacgatcg
     9841 tacgttagaa ttcgcgctcg cttggggcga tagctcagtt gggagagcat ctgcatggca
     9901 tgcagaaggt ccggggttcg agcccccgtc gctccaccaa accagtccga gccagggggt
     9961 catggccaag gacgccacag gcaagcgaaa cacgcgtcgc cgcattgcgg tgtgcgcggt
    10021 cgtgctgctc gtgggttgcg gaggtccggc acactcaccg tcgggtcagg acagcgtcgc
    10081 ttcggaaggc tctaatccgc gcgttacgga tgcggtcgcg ccttcacctg gcccgagcag
    10141 cgcgcagctg gtggcgtcga ccctcacgtt ccaacaggat gaccggctgg cgacacacgg
    10201 caccctgacg ccggaaacgg gtggaacact ggaagtcacg gccgccgacg ggacgcggtt
    10261 tcggctggac gttccgccgg gagctgtctg ggaagagaca gaggcgtcgg tgacgccact
    10321 catcgacgtc cacggggtgg gtgacggtcc cgtgcatgct gtccggctcg agccagaagg
    10381 catcaccttg ttcgaagaag cgcgcctcct ggtcttaccg gcgacgccca tcgccgtcgc
    10441 ggagcagctg atatttcacg cacgcggcga cggctccaat gcactggccg cgctagtcga
    10501 ccccgaggca tcggacgtcg tcatcctgct gcagcatttc tccatcgccg ggacggcaaa
    10561 ggctgcagca gctgcttcgt ggaacattgg ccagtctcat tcggcagtgg aacgcctctc
    10621 gaatgaggta ggcaagtacg ttcaagaaca gcgaaagcga gcagcgagag gcgatgacgg
    10681 actgtccgag gtgagcgagg tcctcgaacg cctgctcagg gacgccgagc gcgacgtgat
    10741 cgcaccgcta cgcgaggcgg cgctcctgac atgcgaggga gccaccgagc tgatgaagat
    10801 gcttcgcgcc gtggagaggc agcgcgcact cgtcggaatt agcacggagg caacattcgg
    10861 agccatgctc gctgaggtca atcgaatttg gacgtcatcg ttcgcgatct gcgaacgcga
    10921 ggccgtcgtg aagtgccagg tccatcccga tccggggctg ctcgtcgagt tctggatcac
    10981 ctgggatcgc agccggtcac tcgccggtca gacccacatg agccccgacg gcgatttggc
    11041 gaagctggca cgagacaaat gcggctggtc gtattccttc aaccgaacgc ttgagatggc
    11101 gcagggtcaa atgtcggtgc ggtacgagtg gaccgggatc gtccgcgtcg cagatgaggg
    11161 cgttatcagc ggcacgggga ccggattcct acgcggttcc caggggacgt gcacatacgg
    11221 cgacccgccc acggaagttc caatcgatgt cacgtccagc gccacgtttg cgttcgagtt
    11281 ccaggggagg cgggccgggg acacactcac tatcgagcac ccggcgatca cggcagacgt
    11341 agttgtcgaa ggcagctttg cgccgtgcat ggaggcgggg ccgctcgcgg agttcgtcat
    11401 cgagacggca ctgcgagatc ccgcgctgct cccgccggca gtcgtggccg atcgggtggg
    11461 ccggacgacg gagcggagaa atggagctac gtcggtcaca accctcagcc cgccacccac
    11521 gccgcggccg acgccgagcc cttccagcca gccgcctgtg gtcggcccat cgacgccgtg
    11581 aggggctgcc cacaatcctg atctgcggtt aagatcacgg cctcatggca cagaggcgag
    11641 tctgggtccg ccccacgcgt cgtgacctcg tacttctcgg ggtcgccggc gcttaacgac
    11701 gcgctgcgcg acccaagaac agatccgtcc cctcctgtca ccaccctaac gagggccatg
    11761 ccatgacccg cgacatcgag cgctacgacc ccatcgagat agaaccgcgc tggcagcagc
    11821 gctgggacga gatgggtctg cacaaga
//
LOCUS       DATLCO010000139         4295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3904727, whole genome shotgun sequence.
ACCESSION   DATLCO010000139 DATLCO010000000
VERSION     DATLCO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3904727"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..89
                     /locus_tag="VMZ33_03450"
     tRNA            15..89
                     /locus_tag="VMZ33_03450"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Asn,seq:gtt)
     gene            121..1509
                     /locus_tag="VMZ33_03455"
     CDS             121..1509
                     /locus_tag="VMZ33_03455"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HUR16318.1"
                     /translation="MPGSTALVWYRRDLRVHDHPALGHATAGFDRVVPVFVVDEAILA
                     GRWPSPNRWWFLARALAALRKDIESRGGRLVIVRGDPVQEVPRLARELGASAVLVSRD
                     YTPFGRARDERVERALRGVGVEWRAGRGVLAHEPEDVLRDGTGAYHVFAPFHRRWLAQ
                     PSRPVLDAPRRIASGDVPLNLLGDPDDLLRHPVPTADQTLLLEPTEDAARQRLATWSR
                     SDELRDYDTGRDRLDRDGTSRISQDLRWGLLSPVEVLARCDGADSGSTRFRTEIAWRD
                     FYAHLLFHEPNVARQSFRAELDGIWNPADESMVAAWQQGQTGYPVVDAAMRQVSAMGW
                     MHNRGRMIVASFLTKHLLIDWRVGESWFMRSLVDGDPASNNGGWQWAASTGTDPQPYF
                     RIFNPTLQGRQHDPDGEFVRRWVPELRGMSDADIHEPPPGTYIAPIVEHRTARAAALA
                     AYEAKQGPKKGR"
     gene            complement(1520..2584)
                     /locus_tag="VMZ33_03460"
     CDS             complement(1520..2584)
                     /locus_tag="VMZ33_03460"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HUR16319.1"
                     /translation="MTAPAMTSRPTAVRDRPTIAVVGATGLVGRTMLQVLIERGFPAR
                     EVRALASARSAGTPLSVGGLDLTVAEATGDAFEGVDIALFSAGAEQSTVLAPLAVERG
                     TVVIDNSSAWRMDPEVPLIVSQVNGDDLSWHKGIVANPNCSTMQLAPLLAALRDSVGL
                     KRVIVDTYQSVSGTGSEAVEELESQVRAYAAGEPLEAKVYPHQIAFNALPEIDVFLDN
                     GYTREEWKVVAESRKILHLPDLPVSCTAVRVPVFFAHSEAVHVETERPIGPTDARELF
                     AAVAGVVVVDDPASHSYPLAIDAAGRDEIFVGRVRGDVSLAQGHGIAFWVVSDNLRKG
                     AATNAVEIAELVVQGGLLKR"
     gene            complement(2581..3870)
                     /locus_tag="VMZ33_03465"
     CDS             complement(2581..3870)
                     /locus_tag="VMZ33_03465"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HUR16320.1"
                     /translation="MAAIETVTNGAARDVAPAVPMNGSVARPIVVVKYGGSSLATSAH
                     VNSVADRLVADSADGARRVVVVSAMGDTTDDLLTLAGEITELPDPRELDFLLSTGEQV
                     SAALMVMALHERGLPAVSLTGAQAGIQTDGLHGRARITGVTPDRIKRELDEGRVAVVA
                     GFQGSSGAEVATLGRGGSDTSAVALAIAMGAVRCEIFTDVEGVYSADPRLVPDARQLP
                     EVGFEEMLELAHQGAKVLQTRSVELAWVHGLEVAVRKTGSDGRGTQIREVAMENRQKV
                     RGIATDDHLAKLTLLELGDRPGMAAHVFAPLAEAGIHVDGIVQNIGRGGRADLSFIVP
                     EADLARAETLLRSMLPDLGARDLAVAGGLAKVSIVGAGIQSAPDYPARMFAALGEAGV
                     NIEMIMTSDIRITCVIARDHLQAAAQALHRTFQLEEA"
     gene            complement(3922..>4295)
                     /locus_tag="VMZ33_03470"
     CDS             complement(3922..>4295)
                     /locus_tag="VMZ33_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16321.1"
                     /translation="GLAVQVFLIGFGMRELGNSPDAISLHRNFGYLLHLSVLGVLLFA
                     YLSRAGRRHWLWALALTVVVLIFPLLVPLRSTYPVLAALHPVGAAVAFALATIVAINA
                     LGLFRRDRATGARTASQGSTE"
BASE COUNT          721 a         1444 c         1436 g          694 t
ORIGIN      
        1 gttgacgcgt gcgttccggc gtagctcagt ggtagagcgg gcggctgtta accgcttggt
       61 cgtaagttcg aatcttaccg ccggagccaa cttccctggc catctctacg atgcccgccc
      121 atgcccggat caacggcact cgtctggtac cgacgcgatc ttcgcgtcca tgatcacccg
      181 gccctcggcc atgccacagc gggcttcgac cgagtcgtcc ccgtgttcgt tgtcgacgaa
      241 gccatattgg ccggacgctg gccgagcccc aaccgttggt ggttcctggc gcgggctttg
      301 gcggcgctgc gcaaggacat cgagtcgcgt ggcggccgat tggtcatcgt gcgcggtgac
      361 cccgtccagg aggtgccgcg gctcgctcgc gagctcgggg cgtcggccgt cctggtaagt
      421 cgcgattaca cgccgtttgg ccgcgcccgc gacgaacgcg tcgagcgcgc gctccgtggc
      481 gtcggggtcg aatggcgtgc tggccgcggt gtcttggccc acgagccaga ggacgtgctc
      541 cgtgacggca caggtgcgta ccacgttttc gcgccattcc accgccgttg gctcgcgcag
      601 ccgtcccgtc ccgtactcga cgcgccccgg cgcattgcgt cgggcgacgt gccgctcaac
      661 cttcttggtg atcccgatga ccttctccgt caccccgtgc caacagccga ccaaaccctg
      721 ttgctcgaac ccaccgagga cgccgcccga caacggctcg ccacatggtc gcgctcggat
      781 gagcttcgcg actacgacac cggtcgcgac cgtctcgacc gcgatggcac atcgcgtatc
      841 tcgcaggacc tgcgttgggg gctcttgtcg ccggtcgagg tgctggcgcg ttgcgacggc
      901 gcggacagcg gttccacgcg gttccgtact gagatcgcgt ggcgcgattt ctacgcgcac
      961 ctgctattcc acgaaccgaa cgtcgcgcgg cagtcgtttc gcgcggagct ggacggcatc
     1021 tggaatcctg cagatgaatc catggtcgcc gcgtggcagc aggggcagac cggataccca
     1081 gtcgtggacg cggcgatgcg acaggtcagc gcgatgggtt ggatgcacaa ccgcgggcgg
     1141 atgatcgtgg cctcgttcct gacgaagcat ctgctgatcg actggcgcgt aggggagtcg
     1201 tggttcatgc gcagcctcgt cgacggcgat ccggccagca acaacggcgg ttggcagtgg
     1261 gcggcgtcga ccggcacgga tccccagccc tacttcagaa ttttcaatcc gacacttcag
     1321 ggtcgacagc acgatcccga tggcgagttc gtgcggcgtt gggtgcccga gctgcgcgga
     1381 atgagcgacg ctgacatcca cgagccgccg ccgggcacgt acatcgcacc gatcgtcgaa
     1441 caccgcaccg ctcgcgctgc ggctctcgcg gcgtacgaag ccaaacaagg gccgaaaaag
     1501 ggacgatgaa ggcgcgcggc tagcgcttca gcaagcctcc ctggacgacc agctccgcga
     1561 tctcaaccgc gttcgtggcg gcgcctttgc ggaggttgtc ggagacgacc cagaaggcga
     1621 tgccgtgccc ttgggcgagc gatacatcgc cccgcacccg tccaacgaat atctcgtcgc
     1681 gtccggctgc gtcgattgcg agggggtagc tgtgcgaggc cggatcgtcg acgacgacca
     1741 cacctgcaac cgcggcaaac agttcgcggg cgtccgtcgg gccaatcggc cgttccgtct
     1801 ccacatggac ggcttcggaa tgcgcgaaga acacgggcac ccgtactgcg gtgcatgaaa
     1861 cgggaagatc gggcaggtgc aggatcttgc gtgactcggc gacgaccttc cactcttccc
     1921 tggtgtagcc gttgtcgagg aagacgtcga tttccggcag cgcgttgaac gcaatctgat
     1981 gcggatagac cttcgcctcg agcggttcgc cggcggcgta cgcgcggacc tgcgactcga
     2041 gctcctcgac cgcctctgaa cccgtgcccg acacagactg gtacgtatcc acgatcacgc
     2101 gcttcagccc gacgctgtcc cgtagcgcag ccagcagggg tgcgagctgc atcgtcgagc
     2161 agttgggatt ggcgacgatg cccttgtgcc acgacaggtc gtcgccattg acctggctga
     2221 cgatgagcgg tacctcaggg tccatgcgcc acgccgacga gttgtcgatg acgaccgtac
     2281 cgcgttcgac agcaagcgga gcaagcacgg tggactgctc ggcgcccgcg gaaaagaggg
     2341 cgatatcgac accctcgaag gcatcgccgg tcgcttcggc gacagtgagg tcaagccccc
     2401 cgacagacag cggtgtgccg gccgatcggg cggaggccag cgcgcgcact tcgcgagccg
     2461 gaaagccgcg ctcgatgagc acctggagca tcgtccgacc gacgaggccg gtggcgccca
     2521 cgaccgcgat ggtcggccgg tcgcggacgg cggtgggccg cgacgtcata gcgggcgcgg
     2581 tcatgcctcc tccagctgga atgtgcggtg gagtgcctgc gctgcggcct ggaggtgatc
     2641 gcgcgcgatg acgcacgtga tacggatgtc ggaggtcatg atcatctcga tattcacgcc
     2701 ggcttcaccg agtgcggcga acatccgcgc cgggtagtcg ggtgcgctct ggatgccggc
     2761 gccgacgatg cttaccttgg ccaggccacc ggcaacggcg aggtcacgcg cgcccaggtc
     2821 cggcaacatg ctccggagca gagtttcggc gcgcgccaga tccgcctcag gcacgatgaa
     2881 cgacaggtct gcccgaccgc cacgccctat gttctggacg atcccgtcga cgtggatccc
     2941 ggcctcggcg agtggcgcga acacgtgcgc agccatcccc ggccgatcgc ccagctcgag
     3001 gagcgtgagc tttgccaggt gatcgtcggt ggcgatgcca cgcaccttct gccggttttc
     3061 catcgcaacc tccctgattt gtgttccccg tccgtcgctg ccggtttttc ggactgccac
     3121 ctcgagtccg tgaacccagg ccagctccac cgagcgtgtt tgaaggacct tcgccccctg
     3181 atgggcgagc tcgagcatct cctcgaagcc gacctcgggg agctgccgcg cgtcggggac
     3241 cagccgcggg tcggcagagt agacgccttc aacgtccgtg aagatctcgc accgcacggc
     3301 gcccatggcg atggccaggg caacggcgct cgtgtccgat ccaccgcggc ccagagtcgc
     3361 gacctcggcg ccgctcgaac cctggaaacc ggcgacaacc gcaacgcgac cctcatcgag
     3421 ctcgcgcttg atgcggtctg gcgtcacgcc cgtgatgcgc gcccggccgt gcagcccgtc
     3481 ggtctgtatg ccggcctgcg cgccggtgag gctcaccgcc ggcagtccgc gctcgtgcag
     3541 cgccatgacc atcagcgcgg cactgacctg ctcgcccgtc gagaggagga agtcaagctc
     3601 gcgcgggtcg ggcagctccg tgatctcgcc ggcaagcgtc aggaggtcgt cggtggtgtc
     3661 acccatcgcc gagactacga ccacccgccg cgcaccatcg gcgctgtccg caacgaggcg
     3721 gtccgcgaca gagttgacgt gcgctgacgt ggcgaggctg gagccaccgt acttgacgac
     3781 gacgatcggc cgtgcgacgg acccgttcat tggcacggcc ggcgcaacgt cgcgcgcggc
     3841 tccattcgtg acagtttcga tggcggccat gggccgcgaa tggtacggct ggcggcgcgc
     3901 agtgcggcta gcccgacagg gctactccgt actgccctgg ctggcggtgc gtgctccggt
     3961 cgcacgatcg cggcgaaaga gaccaagggc gttgatcgcc acgatggtcg ccagggcgaa
     4021 cgcaacggcg gcacccacag gatgaagcgc tgccaggacc gggtacgttg accggagcgg
     4081 aacaagcagc ggaaaaatga ggacgaccac cgtcagtgcg agcgcccaca gccagtggcg
     4141 gcggccggcg cgcgacagat acgcgaacag cagcaccccg agcaccgaaa ggtggagcag
     4201 gtaaccgaaa ttgcggtgca ggctgattgc atcgggagag ttgcccaact ccctcatccc
     4261 aaagccgatc agaaacacct ggaccgccag ccccg
//
LOCUS       DATLCO010000140         6570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_182809, whole genome shotgun sequence.
ACCESSION   DATLCO010000140 DATLCO010000000
VERSION     DATLCO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182809"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="VMZ33_03475"
     CDS             complement(<1..1643)
                     /locus_tag="VMZ33_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR16322.1"
                     /translation="MSSGARLTALRLVGFKSFAEKTTIEFGPGVSAVVGPNGSGKSNL
                     ADALRWTMGEQGRMLRTRRAEDVIFAGSSSRRAIGMADVTLVIDNSDRLLPVDFGEVE
                     IGRRLYRSGESEYLLNRQRIRLRDLTELLDEGNLADNAFLFIGQGMVDQALALRPEER
                     RPLFEEAAGVRRHERRRRQAEAELTEAEANLERLRDLLGELRPQAKRLAAQSEQLQAR
                     RTAGLELAEVLLAAARSRWLDVATAEGREARALDAARNASDKALAELHAAEQLATQTS
                     AEMASHADEERARRAVLERERADVVEHRVKLARIESELSSLDEAIQRARSDREAIAVR
                     LREAAVGSSVPQPTVDQAIATELEAVDRELAVLSAEGRGPDANAELTARRAEHDRHQR
                     RSSELEQNLLAAQAELLRLGQRLTEAQTEHSATTAQLTEATAVVVAAEHSLESARTSQ
                     SAAAAEHTHVRGSRAELESEVVAARSRLSALESALEAMSDEGLARAARARGAKLVAEG
                     VEIDARFRRAVAAVLDSAATAFVVDDPGPLAAPSRRGALV"
     gene            complement(1640..2374)
                     /gene="rnc"
                     /locus_tag="VMZ33_03480"
     CDS             complement(1640..2374)
                     /gene="rnc"
                     /locus_tag="VMZ33_03480"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HUR16323.1"
                     /translation="MAPAKELAERLGLEFDDLGLLSQALVHSSYVHEQPLEGPSNERL
                     EFLGDSVISIIVSEALFQRYPDEDEGSLTTRRAAIVSARGLARIAQRLELDTYLILGL
                     GATAAGEKSRKSVLAATFEAVTGAIYLAHGMDGTRQWLLDVAAPELEGRTDVDDLKPP
                     KSVLQELSYRRTGRAPQYRTISEEGPPHARHYVVEALVAGESLGRGEGRNRRDAETEA
                     AREAMTALDAAKSARPTAKQESEPEQ"
     gene            complement(2401..2640)
                     /gene="acpP"
                     /locus_tag="VMZ33_03485"
     CDS             complement(2401..2640)
                     /gene="acpP"
                     /locus_tag="VMZ33_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUR16324.1"
                     /translation="MTASTYERLKKIVVEQLGVDEEQVTPEANFVDDLNADSLDLVEL
                     IMSLEEEFGAEISDEDAERIRTVQDAVDYVEERAT"
     gene            complement(3115..4146)
                     /gene="plsX"
                     /locus_tag="VMZ33_03490"
     CDS             complement(3115..4146)
                     /gene="plsX"
                     /locus_tag="VMZ33_03490"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HUR16325.1"
                     /translation="MDVGILRSTDEESSGPVRVAVDAMGGDHAPEEVVAGSLDWARLN
                     PDTQLLLVGDEARVRALVGLEGLPENVTIVHASESIGMDEHPAAAIRSKRDASINVCM
                     RLVREGRADAVVTAGHTGAGVASAIINLGRIKGVDRPALAVQMVTDSGPMVLLDIGAT
                     TDSTGTNLFQYAHMGTIFAEKVIGVSNPSIALLSIGEEGGKGEQRVQEATVLLKDSKL
                     RFLGNVEGRDLPKHPADVIVCDATVGNVTIKFFEGLSSFIFDQLRTEFRRWPLGPIGY
                     AFMRPGLGRIRKRFDYEKLGGAPLLGVKGTVLITHGRAKRRMIGFAVDVTAAAARARI
                     PERIADALA"
     gene            complement(4194..4400)
                     /gene="rpmF"
                     /locus_tag="VMZ33_03495"
     CDS             complement(4194..4400)
                     /gene="rpmF"
                     /locus_tag="VMZ33_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HUR16326.1"
                     /translation="MGVPKRRVSHARQGDRRAHHALTVPQLEECPHCKVQKRAHHACL
                     NCGWYNGREAVQIKAKTKSDTAAS"
     gene            complement(4409..4939)
                     /locus_tag="VMZ33_03500"
     CDS             complement(4409..4939)
                     /locus_tag="VMZ33_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HUR16327.1"
                     /translation="MLSYNVSALLRATPGTERRYPIEVIELSIADDLRLAKPIEGEVR
                     LSRTGRSIIARAHVTTAIDGSCARCLQPVVAPIEVSIEEEALPSVDINTGLPLDKDAE
                     PDALRLDDHHELDLVEPIREAISLAEPITILCRPDCMGLCLVCGIDLNNDPDHKHSDD
                     DIDPRFAALADWQTRR"
     gene            complement(4932..5522)
                     /locus_tag="VMZ33_03505"
     CDS             complement(4932..5522)
                     /locus_tag="VMZ33_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16328.1"
                     /translation="MAQAPAAQPHGQRPGHGDDPIDIVFLIEQLERLISGGRSMPLTS
                     NVIIDQTRALDLLDQLRVAVPEEVRAAKRLNDETERIVERAQEEAERILARAQEQAAF
                     LIEERELTKAAEIKSAEIIGEGEHEADEIRRGADDYAANVLVKLEGTLIKELQSIKRG
                     LAMLDERHGADSGAGPDELQDDDREPEVERLERTHA"
     gene            complement(5681..6163)
                     /gene="coaD"
                     /locus_tag="VMZ33_03510"
     CDS             complement(5681..6163)
                     /gene="coaD"
                     /locus_tag="VMZ33_03510"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HUR16329.1"
                     /translation="MTTAIYPGSFDPITCGHIDVIGRAAGMFDRLVVAVLVNPKKTPF
                     LELEERIAVIREAIDEELPAPAGGIQVDSFDGLTVDYARRIGARFIVRGLRAVSDFES
                     EMQMAHTNRKLHPDVDTIFFMTSLEHAYLSSTLVKEIASFGGDVSQMIPAAAARRLHR
                     "
     gene            complement(6165..>6570)
                     /locus_tag="VMZ33_03515"
     CDS             complement(6165..>6570)
                     /locus_tag="VMZ33_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HUR16330.1"
                     /translation="GAAGIEALSRGAPAATFVEKDGKTCAVIGANLRRAKLGGGNVVR
                     ADVSNYLREAIRATTAQFTAVVLDPPYGEPQLLLASLEALADAERGLLASEAIVVAKH
                     FWRDDLTEVVGNLVKSRQKRFGETMLTFYTRN"
BASE COUNT         1055 a         2200 c         2134 g         1181 t
ORIGIN      
        1 acgagagcac cacgtcgcga tggcgccgcc agcggacccg gatcgtcgac gacgaacgcg
       61 gtcgccgccg agtcgaggac ggcggcaacc gcgcgccgaa agcgcgcgtc gatctcgacg
      121 ccctcagcga cgagcttcgc tccacgcgcc cgcgcggcac gcgccaggcc ctcatccgac
      181 atggcctcca gcgccgattc gagggcactc aaccgcgacc gcgctgcgac cacctccgac
      241 tcgagctccg cgcgcgagcc acgcacgtgg gtatgctccg ctgctgccgc cgactgcgat
      301 gtccgggcgg actcgagcga gtgctccgcg gcaaccacta cggctgtcgc ctctgtgagc
      361 tgagccgtcg tcgcggaatg ttcggtctgg gcctcagtca gccgctgccc cagtcgcagc
      421 agctcggcct gggcggccaa gaggttctgc tccagctccg agctacggcg ctgatgtcgg
      481 tcgtgctcag ctcgacgcgc agtcagctcg gcattcgcgt cgggaccacg cccctcagcc
      541 gacaggacgg ccagctcgcg atcgacggcc tcaagctcgg tcgcgatcgc ctggtccacg
      601 gttggctgcg gcacgctgga gccaactgcc gcttcacgga gccgtacggc gattgcctca
      661 cgatcggaac gagctcgctg gatcgcctcg tcgaggctgg aaagctcgga ctcgatgcga
      721 gcgagcttca cgcggtgctc gacgacgtcc gcgcgctcac gctcaagcac agcccgacgg
      781 gcccgctcct cgtccgcgtg tgacgccatc tccgccgatg tctgcgtggc cagctgttcg
      841 gccgcgtgaa gctcggccag agccttgtcg gaagcgttgc gtgccgcgtc cagggcgcgt
      901 gcctccctgc cctccgcagt tgctacatcg agccagcgcg accgcgcggc cgcgagcaac
      961 acctcggcca gctcgaggcc agccgttcgc cgagcctgaa gctgctccga ctgggccgcg
     1021 agccgctttg cctgcggacg cagctcgccg agcaggtcgc gcaaacgctc gaggttggcc
     1081 tctgcctcag tcaactcggc ctccgcctgc cgccgtcgtc gctcgtgccg ccgtaccccg
     1141 gcggcttcct cgaatagtgg ccgccgctcc tccggcctga gcgcaagagc ctggtcgacc
     1201 atgccctggc cgatgaacag gaacgcgttg tcggcgaggt tgccctcgtc aagaagctcg
     1261 gtcaggtcgc gcagcctgat ccgctggcgg ttgagcaggt actcgctttc cccagatcga
     1321 tagagacgcc ggccgatctc cacctctccg aagtcgaccg gcagcagccg atcactgttg
     1381 tcgatgacga gtgtcacgtc ggccatgccg atcgctcgcc gcgacgagga gccggcgaag
     1441 atgacgtcct cagcgcggcg ggtgcggagc atccgaccct gctcgcccat ggtccagcgc
     1501 agggcgtcgg ccaggttgct cttgccggag ccgttgggtc cgaccacggc cgagacgccc
     1561 ggtccgaact cgatcgtcgt cttctcagcg aacgatttga agccgacaag ccgcagcgcc
     1621 gtgaggcgtg cgccactgct cattgctcgg gctccgattc ctgtttggcc gtgggtcggg
     1681 cggacttcgc ggcgtcgaga gcggtcatgg cctcacgcgc cgcctcggtc tctgcatcgc
     1741 gccggttgcg accttccccg cggccgagcg actcaccggc gacgagcgcc tccacaacgt
     1801 aatgccgagc gtgcggcggc ccctcctcgc tgatcgtccg gtattgcggg gcgcgacccg
     1861 tgcggcggta gctcagctcc tggagaacgc tcttgggcgg cttcaaatcg tcgacatcag
     1921 tccggccctc cagctctggc gcagccacgt cgagcaacca ctgacgtgtg ccgtccatgc
     1981 cgtgcgcgag gtagatggca cccgtaacgg cttcgaacgt cgctgccagc accgacttgc
     2041 gtgacttctc gccagccgct gtcgcgccga ggccgaggat caggtacgtg tcgagctcga
     2101 ggcgctgggc aatgcgcgcc aggccgcgcg cagacacgat cgccgcgcgg cgcgtcgtaa
     2161 gggagccctc gtcctcgtcc gggtagcgct ggaacagcgc ctccgagacg atgatcgaaa
     2221 tcacggaatc accaaggaac tcaagccgtt cgttcgaagg cccctcaagg ggttgctcat
     2281 gaacgtagct gctgtgaacc agagcttgcg aaagcagccc gaggtcgtcg aattccagcc
     2341 cgaggcgctc ggccagctct ttagccggcg ccattacgtc agtcttgaag gagggggctg
     2401 ctaggtcgcc cgctcctcga cgtaatcgac ggcgtcctga acggtgcgga tcctctcggc
     2461 gtcttcgtcg gagatctcag cgccgaactc ttcctcgagg ctcatgatca gttcgacgag
     2521 atcaagtgag tcggcgttca ggtcatcgac gaaatttgct tcaggtgtca cctgctcttc
     2581 gtcaacgcca agctgctcga cgacgatctt cttcaggcgc tcgtaggtcg acgcggtcac
     2641 ccagtgattc ctcctgtgcg gcggttttgc ccgccctcgt tgctactgcc ggaggcttgc
     2701 ctctcctgcg ttcggtccgc cgcctccgtg gcgacccgtc caagcacccc gttgagcaaa
     2761 cgacgggctg gttcgccact gtaggttcgg gccaattcaa cccattccgc gatggccact
     2821 cgggccggcg tcgtgcggga gtgtagcagt tcagccatcg ccgaacgcaa caaagcgcgg
     2881 tccattctgg ccagttggat gactggatag cgaggggcga gagcctctat ccgggcatcg
     2941 atttcaactc gatttgcgag aacggccgac acgatcgact gcgccagctc tgccgccgga
     3001 ggtgtcgtcc cctcctccgc tattcgacgc tcgaggatcc gttgagccgg gcgctggccg
     3061 aactcggcct cgaaaaccgc cccgagcgcg aggcggcgcg cctcccgctc gtcgtcatgc
     3121 gagtgcatcg gcaattcgtt cagggatgcg ggcacgggct gcagctgccg tgacgtccac
     3181 ggcgaagccg atcatgcgtc tcttggctcg cccgtgggtg atgaggacgg tgccctttac
     3241 gccgagcagc ggcgcgccgc ccagcttctc gtaatcgaag cgcttgcgga tgcggccgag
     3301 gccggggcgc atgaaggcgt agccgatcgg gccgaggggc cagcgacgaa actctgtccg
     3361 gagctggtca aagatgaagc tcgacagtcc ttcgaagaac ttgatcgtga cgttgccgac
     3421 ggtggcgtcg cagacgatca cgtcggccgg atgcttgggc aagtccctgc cctccacgtt
     3481 gcccaggaag cgcagcttgc tgtccttcag cagcacggtc gcctcctgca ctcgctgctc
     3541 gcccttgccg ccttcctcgc cgatcgacag gagcgctatt gatgggttcg acacgccgat
     3601 caccttctcg gcaaagatcg tgcccatatg cgcgtactgg aagaggttcg tgccggttga
     3661 gtcggtcgtg gcgccgatgt cgagcaacac catcggcccg ctgtcggtga ccatctgcac
     3721 ggccagggct gggcggtcga cacctttgat acggccgaga ttgatgatcg ctgaagccac
     3781 ccccgcgccc gtgtgcccgg cggtgaccac ggcatcggcg cggccctcac gtaccaggcg
     3841 catacagacg ttgatgctcg cgtcgcgctt gctacgaatg gccgcggcgg ggtgctcatc
     3901 catgccgatt gactctgaag cgtggacgat cgtgacgttc tcgggcaaac cctcgagacc
     3961 cacaagtgcc ctgacacgtg cctcgtcccc gacgagcagc agctgggtgt cgggattcag
     4021 gcgtgcccaa tcgagcgagc ccgctacgac ctcctccggt gcgtggtcgc cacccatggc
     4081 gtctacggca acacgaacag gcccgctgga ctcctcatcc gttgagcgaa gtattccgac
     4141 gtccatctac ccactccgtc ccatggacgg cgcggcgggc cggagcctgg ccgtcaggaa
     4201 gcggccgtgt cggacttcgt cttggccttg atctgaacag cctcgcggcc gttgtaccag
     4261 ccacagttca ggcaggcgtg gtgcgcgcgt ttctgcacct tgcagtgcgg gcactcctcg
     4321 agctgcggca cggtgagcgc gtgatgcgcg cgccggtcgc cctgacgggc gtgtgagacg
     4381 cgcctcttgg gaactcccat ttctttctct atctcctcgt ttgccaatcg gccagcgcgg
     4441 cgaaccgcgg gtcgatgtcg tcgtccgaat gtttgtggtc cggatcgttg ttcaagtcga
     4501 tgccgcagac aaggcacagg cccatgcagt cgggacggca caggatagtg atcggctccg
     4561 ccagcgagat cgcttcgcgg atcggttcaa cgagatccag ctcgtggtgg tcgtccaagc
     4621 gtagagcatc aggctccgca tccttgtcga ggggcaagcc ggtgttgatg tcgaccgatg
     4681 gcagcgcctc ttcctcaatg ctgacctcga tcggcgccac gaccggctgc aggcaccgcg
     4741 cacagctgcc gtcgatggcc gtcgtgacat gggcccgagc gatgatgctg cggccagtcc
     4801 gcgagaggcg aacctcgccc tcgatgggct tggccaggcg aaggtcatct gcgattgaaa
     4861 gctcgatgac ctcgattggg taccgccgct cggtacctgg cgtcgcacgc agcagggcgg
     4921 acacgttgta gctaagcatg ggtgcgctcg aggcgctcca cctcgggctc gcggtcgtcg
     4981 tcctgaagct cgtctgggcc cgcgccgctg tccgcgccat ggcgctcgtc gagcatggcc
     5041 agaccgcgct tgatgctctg cagctctttg atgagcgtgc cctcgagctt gaccagaacg
     5101 ttggcggcgt agtcgtcggc cccgcggcgg atctcgtcag cctcgtgctc accctcgccg
     5161 atgatctcgg cagacttgat ctccgccgcc ttcgtcagct cgcgttcctc aatcaggaac
     5221 gcggcctgtt cctgagcgcg cgcgaggatg cgctcggcct cttcctgggc acgctcgacg
     5281 atccgctccg tctcgtcgtt gagccgcttc gcagcgcgga cttcctcagg cacggcaaca
     5341 cgaagctgat cgagcaggtc gagtgctcga gtctggtcga tgatcacgtt gctggtcaga
     5401 ggcatgctgc ggccgccgct gatcagccgt tcgagctgtt cgatgagaaa tacgatgtct
     5461 atggggtcgt ctccgtggcc cgggcgctgg ccgtgtggct gcgctgcggg ggcctgcgcc
     5521 atcaacgtcc gattgtacgg cctagcaagg cgtagctagt cggtggcgcg agcgacagac
     5581 tcgcggagcc gcgcagcagt ggcgcgcagg ccgactgcga agcctgactt cgctccaagg
     5641 cgtagctagt cggtcgcgcg agcgacagac tcgcggaggg ctagcggtgc aggcgacgtg
     5701 ccgcggccgc cgggatcatc tggctgacgt cgcctccgaa cgaggcgatc tccttgacga
     5761 gcgtagatga gagatacgcg tgctcgaggg aggtcatgaa aaagatcgtg tcgacgtccg
     5821 ggtgcagctt ccgattggtg tgcgccatct gcatctcgga ctcgaagtcg ctcaccgcgc
     5881 gtaggccacg gacgatgaag cgggcaccga tgcgacgtgc atagtcgacc gtcaaaccgt
     5941 cgaagctgtc gacttggata ccgccggcag gcgctggtag ctcctcgtca atggcctcgc
     6001 ggatgacggc gatgcgctcc tcgagctcca ggaacggcgt cttcttgggg ttgacgagga
     6061 cggcgactac cagccggtca aacatgcccg cagcacggcc gatgacatct atgtggccgc
     6121 acgtgatggg gtcaaacgac ccgggataaa tggcggtggt catcctagtt gcgagtgtag
     6181 aaggtgagca tcgtctcgcc gaaccgcttt tggcgggatt tgaccaggtt gccaaccacc
     6241 tccgtcaggt cgtcgcgcca aaaatgcttg gccacgacga tcgcctcgga cgcaagcagt
     6301 ccacgctctg cgtcggcgag ggcctccagc gacgcgagga gaagctgcgg ttcaccgtat
     6361 ggtggatcga gaacgacggc cgtgaactgc gccgtggttg ctcgaatggc ttcgcgaagg
     6421 taattggaaa catcggcacg aacgacgttg ccaccaccaa gcttcgcgcg tcgcagattg
     6481 gcgccgatca ccgcgcaggt cttgccatcc ttctcgacga aggtggccgc gggagcgcca
     6541 cgggatagtg cctcgatccc ggctgcgcca
//
LOCUS       DATLCO010000141         2980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_928365, whole genome shotgun sequence.
ACCESSION   DATLCO010000141 DATLCO010000000
VERSION     DATLCO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928365"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VMZ33_03520"
     CDS             <1..294
                     /locus_tag="VMZ33_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16331.1"
                     /translation="IYERHRHRFEVSNSYREALEGAGMLLSGQSPDGRLVEIVELKDH
                     PWFVASQFHPEFKSRPDRPHPLFDGFVKASIENVSVRSDTDSPIYRETVPAQA"
     gene            314..1252
                     /locus_tag="VMZ33_03525"
     CDS             314..1252
                     /locus_tag="VMZ33_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HUR16332.1"
                     /translation="MAAVLDPFILQYAPGPLAELDPAILRARGDVAAFLETMAQLDDS
                     SLAFQWFWDGNDINVRYAFYRALEVLSAAESAARLALAGAPSSEAREAAAATDAARWD
                     TDGLLVSLNDADLDGDPGNAEWTIRQTMAHMINGQRGYAWGSAAWLSVRDKPRAEGPQ
                     RLPEEMFNDLPEEDQEARGSLAEVRQELADIIDATSSRYATLTDDEMGVRSGWYGFPV
                     TVGFRQWRWSSHVSEHTVQVEKTLDMLGEGQSEVARLTRLVARAYGRLESVVFYREGA
                     SASAGAPILDGVAAELRNIRETVPAAIEAGVPANND"
     gene            complement(1273..2247)
                     /locus_tag="VMZ33_03530"
     CDS             complement(1273..2247)
                     /locus_tag="VMZ33_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR16333.1"
                     /translation="MGKRYEVHAFAADDIDDAAELLAARHQRQRAAWPALNPAFEDAA
                     IVQSLLTGLLWDETASGWSVAVEGRTSAYLIGARKPEVPWGQNVWVGDFANAGDDPEA
                     IRHVYATAAAAWAADGRVQHYVTVPSTDAATVEAWFSLSFGLQHVYAFREPAAADFQP
                     WTGNGLTIRPAENSDIPALLELDPVLPTHSGQSPVFSPVPIPPREESAAEIEQDLSDN
                     RFQSFVAERGGHVIATATAVSLDMSNSWSPLMRPVSCGFLGFAAVLPHARGLGAGRAL
                     ADAVLAWSRDQGFEWVATDWRSTNLEANRTWRAAGFRPAFYRLHRAIG"
     gene            2320..>2980
                     /gene="fsa"
                     /locus_tag="VMZ33_03535"
     CDS             2320..>2980
                     /gene="fsa"
                     /locus_tag="VMZ33_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HUR16334.1"
                     /translation="MKIFLDTADIEEIRIAARWGVLDGVTTNPTLYGKIGGSYEDILK
                     AICELTPGPVSAEVVAEHVDGMLDEGRAFAKLAPNIVVKVPMSEEGLEAIYRLAEEGI
                     KTNCTLIFTANQGLLAAKAGASLLSPFVGRLDDINQDGMDVIRELVTMVENYGLDTEV
                     LAASIRHPRHMTEAALLGAHICTTPFKILQQMVHHPLTDKGIVQFRQDWDKARAAAAE
                     KN"
BASE COUNT          549 a          987 c          966 g          478 t
ORIGIN      
        1 atctacgagc ggcaccgcca ccgtttcgag gtcagcaact cataccgcga ggctctcgag
       61 ggggcgggga tgctcctgtc cggccagtca cctgacggcc ggctggtgga gatcgtcgag
      121 ctcaaggacc acccgtggtt cgtggccagc caatttcacc ccgagttcaa gagccgaccc
      181 gaccgcccgc acccactatt cgacggcttc gtgaaggctt cgatcgagaa cgtcagcgtc
      241 cgatccgaca cggacagccc gatctaccgc gagacggttc cggcccaggc ctgacccagc
      301 caccaaagca gacatggccg cggtcctcga tccattcatt ctccaatacg cgccggggcc
      361 gctcgctgag ctggacccgg ccatcctgcg cgcgcggggc gacgtcgctg cctttctcga
      421 aaccatggcg cagctcgacg attcgagcct tgcgttccag tggttctggg acggcaacga
      481 cataaatgtc cgctacgcgt tctatcgggc gttagaggtg ctgtcggctg cggaatcagc
      541 cgcccggctg gcgctcgcag gtgcgccctc gagcgaggca cgggaggctg ccgccgccac
      601 agacgccgcg cggtgggaca ccgacgggct gctcgtgtcg ctcaacgacg cagacctgga
      661 cggggatcca gggaacgccg agtggacgat tcgccagacc atggcacaca tgatcaacgg
      721 ccagcgcggc tatgcctggg gttccgcggc ttggctgtca gttcgcgaca agccaagggc
      781 tgagggtcct cagcgcctgc ctgaggagat gttcaacgac ctgcccgaag aggatcagga
      841 ggcgcgcggc tcgctcgccg aggtacggca ggagctggct gacatcatcg atgccacgtc
      901 ctcgcgctac gcgaccttga ccgacgatga gatgggtgtg cgcagtggct ggtacggctt
      961 tccggtgacg gtgggctttc ggcagtggcg ttggtcgtcg cacgtcagcg agcacaccgt
     1021 ccaggtagag aagacgctgg acatgctagg cgagggtcag tcggaggtgg cccggcttac
     1081 tcgcctcgtc gcacgcgcgt atgggcggct ggaatcagtc gtgttctatc gcgaaggcgc
     1141 cagcgccagc gccggagccc cgatcctcga tggcgtggcg gccgagctcc gcaatatccg
     1201 cgagacggta cccgcggcca tcgaagcggg agttccagcg aacaacgatt aacggcgccc
     1261 gggccgctgc ggttagccga tggcgcggtg gagccggtaa aaggccggtc tgaagccagc
     1321 ggcgcgccac gttcggttcg cctccaggtt cgtggaccgc cagtcggtag cgacccactc
     1381 gaatccctga tcgcgcgacc acgccagcac cgcgtccgca agcgcgcgcc cagcaccgag
     1441 accccgtgca tggggcaaca ccgcagcgaa acccaaaaag ccgcagctga cgggccgcat
     1501 cagcggcgac caggagttcg acatatccag ggaaacggcc gtggcggtag caatcacgtg
     1561 gccgccgcgc tccgccacga aggattggaa ccggttatcg ctcagatcct gctcgatttc
     1621 cgccgccgac tcctcgcgcg gcgggatcgg aaccggtgaa aagaccggcg attgtccgga
     1681 gtgagtgggc agcaccgggt ccagctcgag cagagccggt atgtcggaat tctccgctgg
     1741 ccggatagtc aggccgttgc ccgtccacgg ctggaagtcg gccgccgccg gttccctgaa
     1801 tgcgtagacg tgctggaggc caaagctcag gctgaaccag gcctcgaccg tcgctgcgtc
     1861 cgtggatgga accgtgacgt agtgctggac gcggccgtcg gcggcccagg cggccgcggc
     1921 cgtcgcgtag acgtgccgga ttgcctccgg gtcgtcaccc gcgttggcga agtcgccgac
     1981 ccagacattt tgcccccacg gaacctccgg cttgcgcgcg ccgatcaggt acgccgatgt
     2041 cctgccttcc acagctaccg accaacctga cgcggtttcg tcccacagca gccccgtcag
     2101 gagcgattga acgatcgctg catcctcgaa cgccgggttg agtgccggcc aggccgcgcg
     2161 ttgccgttgg tggcgggcgg cgaggagctc cgccgcatcg tcaatgtcat ccgccgcgaa
     2221 tgcatgcact tcgtagcgct ttcccaccgc cgcactctac ggattggcag gacgccgcgc
     2281 gtaacattcg aacgagacac gaataggagc gcaacgacca tgaaaatctt tcttgacact
     2341 gccgacattg aagagatccg catcgccgcc cgctggggcg tgctcgacgg cgtgaccacg
     2401 aatccgaccc tctacggcaa gatcggcggt tcgtacgagg acatcctcaa ggccatctgc
     2461 gagctcacgc ccggccccgt cagtgcagag gtagtcgcgg agcatgtcga cgggatgctc
     2521 gacgagggcc gcgcgttcgc gaagctggcg ccgaacatcg tggttaaggt gccgatgagc
     2581 gaagagggtc tcgaagccat ctatcgtctg gccgaggaag gcatcaagac caactgcacc
     2641 ttgatcttca cagccaatca gggcttgctg gctgccaagg ccggtgcgtc gctgctgtcg
     2701 ccattcgtcg gtcgtctgga cgacatcaac caggacggca tggacgtcat ccgcgagctg
     2761 gtcacaatgg ttgagaacta cggcctcgac acagaagtcc ttgccgcttc gatccgtcac
     2821 ccgcgccaca tgacggaggc cgcgttgcta ggcgcccaca tctgcacgac gcccttcaaa
     2881 atcctccagc agatggtcca ccacccgctc accgacaagg gcatcgtcca gttccgccag
     2941 gactgggaca aggcgcgagc cgctgcggcc gagaagaacg
//
LOCUS       DATLCO010000142         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3908702, whole genome shotgun sequence.
ACCESSION   DATLCO010000142 DATLCO010000000
VERSION     DATLCO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3908702"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VMZ33_03540"
     CDS             complement(<1..538)
                     /locus_tag="VMZ33_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16335.1"
                     /translation="MTSPSPTPLPSAAIADPTAVIVKIEQTGGMMPPWETLRRYPSVA
                     LYGDGRLIFQGAQIEIFPGPALPSLWVTHLTERGIEQVLEWAADAGLQGPDRNLGEQI
                     MDAGANVYTVARPEGTHRTVVHSNGSNDPDINALLQFQDVVMNARQWLDEGGVVGEDV
                     PYVADSLRVIAFPAQPQGG"
     gene            734..1690
                     /locus_tag="VMZ33_03545"
     CDS             734..1690
                     /locus_tag="VMZ33_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16336.1"
                     /translation="MDELKGRVMTVTGAVDPAALGVTDAHDHLFLASHAMPGQEFQDS
                     EKAIQEVRTAQAGGLATIVDLTPIGLGRRPDLLRKVSDATGLRVVGATGFHRDEHYPD
                     GHWALEATDDVLSVRMLTDIREGMHPTDWVGGDLLDHARAGVIKLGASYQHISRQERR
                     RLDVGGAVSVQTGVSIVVHTEIGTVGDEIVDVLESVGVPPERIVLAHLDRNPDWEWHA
                     ELLSRGISVVYDTPGRIKYFPDSTLLGLIENVLAAGFGDRVMLGMDLGRRDYFKVYGG
                     GPGLGYLMERFVPRLERRIGAEAVHQILVANPARVLTLSPIA"
     gene            1687..>2014
                     /locus_tag="VMZ33_03550"
     CDS             1687..>2014
                     /locus_tag="VMZ33_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR16337.1"
                     /translation="MIREVDDRYDVIVVGSGSAGSSAAITAGRLGARTLLLDRLAFMG
                     GTSTAVLDTFYAFYTPTDAPRRVVGGLGWEVVERLRDEGVCFERPNTYGAGTGVTYDQ
                     ETLKVTW"
BASE COUNT          316 a          598 c          739 g          361 t
ORIGIN      
        1 caccgccctg gggctgcgct ggaaacgcaa tgactcgcag cgagtcggcg acgtacggca
       61 cgtcctcgcc cacgacgccg ccctcatcga gccactgccg cgcgttcatg accacatcct
      121 ggaactggag cagcgcgttg atatcggggt cgttgctccc gttgctgtgg acgacagttc
      181 ggtgagtgcc ttcaggccgc gcaacggtgt agacgttcgc gccggcatcc attatctgtt
      241 cgccgaggtt gcggtcgggc ccttgcaggc cagcgtcagc ggcccactcg agaacctgct
      301 ctatcccacg ctccgtcagg tgggtgaccc agaggctggg gagtgccgga ccgggaaaaa
      361 tctcgatctg agcgccctga aagatgagtc gcccatcgcc gtagagcgct acggacgggt
      421 accgccgaag cgtctcccac ggcggcatca tgccgcccgt ttgctcgatc ttgacgatca
      481 cggccgtggg atcggcgatc gccgctgaag gaagaggagt gggcgacggg gacgtcatcg
      541 gtgacgggga gggcgtcggg gatggcgccc ccgtcggcga agggctgcct cctggcgagg
      601 cgctacaggc agcgaggagg acgactgctg taacgccgag cgccagccgg gcagtgcggt
      661 tcatgggctt tttgacgcgc gggttgcccg atagttcccg cgatgtcggc tgcggctggc
      721 atcatcgcta ccgatggacg agttgaaggg cagggtcatg acggttaccg gtgctgtcga
      781 tccggccgcc cttggcgtga ccgacgctca tgaccacctt ttcctggcct cgcacgcgat
      841 gcctggacag gagtttcagg actccgaaaa ggcgatccaa gaagttcgaa cggcgcaggc
      901 cggcggcctg gcgacgatcg tcgacctgac gcccatcggg ctggggcggc gacccgacct
      961 gttacgcaag gtgtctgacg ccacgggcct gcgcgtggtt ggtgccacgg gctttcatcg
     1021 agacgagcac tatccggatg gtcactgggc tctggaggcg accgacgacg ttctgtcggt
     1081 gcgcatgctg accgacatcc gggagggcat gcacccgacc gattgggtag gtggcgacct
     1141 gctcgaccac gcgcgggcgg gcgtgatcaa gctcggagcg tcgtaccagc acatctcgag
     1201 gcaggagcgg cgcaggctgg acgtcggtgg cgcggtgagc gtgcagaccg gtgtgtcgat
     1261 cgtggtgcac acggagatcg gcacggttgg cgacgagatc gtcgatgtgc tggagagcgt
     1321 cggcgtgccg ccggagcgca tcgttctggc gcacctcgat cgcaatcccg actgggagtg
     1381 gcacgccgag ctgctgtcgc gtgggatctc ggtcgtgtac gacacgccgg gacggatcaa
     1441 gtacttccct gactcgacgt tgctcgggtt gatcgaaaac gtgcttgccg caggctttgg
     1501 cgatcgggtg atgctcggga tggacctcgg gcgtcgggac tacttcaagg tttacggtgg
     1561 cgggccgggg ctgggttacc tgatggagcg gttcgtgccg cggctggaaa ggcggatcgg
     1621 ggcggaggcc gtgcaccaga tcctcgttgc gaatccggct cgggtgttga ctctgagtcc
     1681 gatcgcgtga tacgcgaggt ggacgatcgg tacgacgtga tcgtggtcgg atctggctcg
     1741 gctggctcgt cggccgcgat cacggcggga cgtctgggtg cgcggacgct gctactcgac
     1801 cgcttggcgt tcatgggcgg cacctcgacg gccgtgctcg acaccttcta cgcgttctac
     1861 acgccgaccg atgcgccgcg gcgcgtcgtc ggcggtctcg gctgggaggt cgtggagcgg
     1921 ctgcgcgacg agggtgtctg tttcgagcgg ccgaacacgt acggtgcggg cacgggcgtg
     1981 acgtatgacc aggagacgct caaagtgacc tggg
//
LOCUS       DATLCO010000143        10898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6800925, whole genome shotgun sequence.
ACCESSION   DATLCO010000143 DATLCO010000000
VERSION     DATLCO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6800925"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VMZ33_03555"
     CDS             complement(<1..688)
                     /locus_tag="VMZ33_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16338.1"
                     /translation="MHDAPSAPQLPALRPPEQQTLATTAAEVNDLFAFMAEAELRFDS
                     LRMRIVDRRYTTHGEELETHEVWVRHPGSAKVITTYGEQTGNAREYDAWMTDGTTVRT
                     FDARAKTATNRRVPLRPVGSDDPRLPGRSRVYLPTTVLPAETLADTFVHPHGYCRNVL
                     ATGVISRRGTQIIAGREAILLRVDHPRTSHVLTDRPDHWLEVGVDFQTGVMLLLAEHI
                     GDRLTRHAQAT"
     gene            756..2267
                     /locus_tag="VMZ33_03560"
     CDS             756..2267
                     /locus_tag="VMZ33_03560"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HUR16339.1"
                     /translation="MTQTRTEKDPLGPLNVPADAYFGVQTQRAINNFPISGRRPDIAL
                     VRAAVQVKKAAARANNQTGRLPDELANAIVRAADEILGLTSTEDGLQQTLVDNFRVDP
                     FQAGAGVSHNMNTNEVLANRAIEILGSQGVGGGQRGDYSVVSPNDHVNMAQSTNDTFP
                     TSMRIATLDRVMELLPVLDELAAAFADRAVAFDGVLKSGRTHMQDAVPIRLGQEFAAY
                     ALTTRRARAAIATAADGLLEQNIGATAVGTGLNAEPEYIALVVELLSEQTGHELRPAE
                     DLVHATQSMAPMLAVSAALRGLAVDLAKICEDLLLMSSGPRTGFAEIRLPARQPGSSI
                     MPGKVNPVMVENLSMISYHVIGADTAVAWAASRGQLELNVMMPLMAHELLESLGILTN
                     GCRQFARECIAGIEADVDRAAWLLEQSSAMATPLAPYIGYALAADIAKEAVKTGKTIR
                     QLVIEKGVFTEAEVNQILDPHELTQPGIAGGFKFEPRMPEGYKRPTGPVGGGG"
     gene            2372..3382
                     /locus_tag="VMZ33_03565"
     CDS             2372..3382
                     /locus_tag="VMZ33_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HUR16340.1"
                     /translation="MPSAASSPFVRRDRRARETQEEGLAPAATRSANSHGRRRHEEPD
                     EYRTSFERDRDRILHSKAFRRLKHKTQVFINPDGDHFITRLSHTLQVTQISRALAAAL
                     TLNETLAEAIALGHDVGHSPFGHTGEEALSPYFPESEWHHAAQSVRIFDVLEDLNLTW
                     EVGDGIRAHSWKIEPPPSTPEAMAVRYGDRIAYLTHDALDAMRGGLLAADDMPARALD
                     RFGPPGRVWVDEMINAVIDHSLATGEVRMDDPTLEVMNELRDFMFERVYLSEAQRLHT
                     QNAIAVIRRLVDYHLEHPDQLPDSYRDTGADLTTQVVDFVAGMTDRYALQVHERLFGL
                     DG"
     gene            3500..4696
                     /locus_tag="VMZ33_03570"
     CDS             3500..4696
                     /locus_tag="VMZ33_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16341.1"
                     /translation="MSKPPTPSGHATPSRRSAVVAFICVFSAVGASAPFLPVYYQSLG
                     LSLDAIGLLAAVAALTALFAAPAWGVVSDRLRSRYVLLVACLGAAISSVLLGLAVEPV
                     VVALCAILYWLWFAGVPPLLDAQALEAVGEDHFQYSRLRVWGSAAFIVSTLVVGALIE
                     RAGLRSLFVVFVGALMLTGLVALRLSTTHARPSMPRLSGLRAVLGNRTAVTFIGIALL
                     TWSASTAVNGFLSIYMIEIGTPGTVIGFAWALGAGVEIPLMIAFPQLARRFGLETLVV
                     AGAAFLLLRVLALLLVHDPLLVAATMTLHGAGFALLLVGGVTYVARHAPSGAEATAQG
                     LLSGVTIGLSQAVGPLFGGALAGVVGLPTMFVFPAVGSAVALAAMVWAVLRPAVSERR
                     AAGSAR"
     gene            complement(4662..5951)
                     /locus_tag="VMZ33_03575"
     CDS             complement(4662..5951)
                     /locus_tag="VMZ33_03575"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HUR16342.1"
                     /translation="MSTTSGSVKVAVVGGGSTYTPELIEGIARRHDRLPVAELALMDV
                     NPERLEVVAGFARRIMERLGWAGVITATDSHEEAVDGASFVLVQLRIGGQQARLTDET
                     IPVRYGTIGQETTGAGGFAKALRTVPVVLELAELTGRRAAPGAWLVDFTNPVGIVTQA
                     LLDAGHRAIGLCNVAIGLQRRMASRFGVEPERVRLGHVGLNHLSWVRHVWVDDIDRLP
                     ELLDGNLTDLANEAGGIPQELVRVLGAVPSSYLRYYYCFNDVLREQESGEHSRAREVL
                     DIEARLLEMYRDPSLTQKPALLENRGGAFYSEAAAQLMASLHDGRGDIQVVDTRNDGA
                     IPELPDDAVVEVPATITREGPRTLPAAALTTDMLALVNHAKGYERLTVQAATTGDRDV
                     ALRALLSNPLVGGYDVASPLLEELLQVNERYLPLFAR"
     gene            6036..6866
                     /locus_tag="VMZ33_03580"
     CDS             6036..6866
                     /locus_tag="VMZ33_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HUR16343.1"
                     /translation="MAKARTLLRSGPATLHHQVYVDLKTAIEEGRYAHGDRLPPERDL
                     AQQYGCSLITVRRALAELAREQRIERTQGRGTFVLRPRIDRDLGDTNSFNEEMARLGL
                     IPETRVLSARSEAAGESVAKALELQPGSPTLHLERLRLASGEPLLLEIVSLPAERFPG
                     LLASDLERGSLYELLSQRYGTEITSARETLEPVLLRMREARLLGQKTRTPALLVEGLA
                     YTSSNVPVEFSRTYVRGDRTRYYVQRVVVREGARPSDRPTQPARGRGQQVAAATSARG
                     "
     gene            6881..8440
                     /locus_tag="VMZ33_03585"
     CDS             6881..8440
                     /locus_tag="VMZ33_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR16344.1"
                     /translation="MARRTGASRIGVLFAVVVLTLGACSGQTATQRPSTGSPGASTPA
                     QTASAAASEPAPSSDASAEPSAEPSAEPSAEPTSDVTAAPSFEPGSGDIRWFCCLGTG
                     EDPEAQIPVEMAAAADFSTEHPDTSMTFEVITYDAARDTLSTMIAGQNAPDLVGPAGV
                     GGIEAYHGQWLDLTPYIEAAEFDLTAYPEALVNFYKHDDGQIGIPFAVYPSMLWYNRD
                     LFDEAELDYPPHEYGAPYVMPDGTERPWDYDTVRELALFLTVDANGNDATSAEFDPEN
                     IVQWGFEPQRDLLASLGAYFGAGSLEASGGAAQVPDAWRDAWKYFYDGMWTDRFIMTG
                     PQFDDIAPDAGGYAFFSGRVAMSTNFLWTTYGVGEDSGVAGVWDLAAVPSHNGTTTSP
                     LNADTFVIHESTDNPEAAFDALTYLQVDRGAELLDIYGGLPARTEEQDAFLAGQAEAF
                     PHDVDWQVVKDSLAYPDNPNFEAFKPAYSETDAILGTYLTRWTTTPGLDLDTEIAALE
                     AEIQAAWDANP"
     gene            8477..9421
                     /locus_tag="VMZ33_03590"
     CDS             8477..9421
                     /locus_tag="VMZ33_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HUR16345.1"
                     /translation="MTTISRARSALRRPKMTPLARREARQGLLFISPWLLGFVLFTVF
                     PMLATLFFSVTNIQLNQEEPLRFVGFDNYSRFLDDKQAIGSLRVTLSFAVLWLPVSIV
                     APFLIALLLNSVHLVGRGVFRVLLFMPYVVPFVAGALIWNSMLGLNGWINEFMRMFGF
                     DIDTPWLRDPSLVYPGLALVGVWGIGGAVIINLAGLRGIPTELYDAAKIDGAGGWAQL
                     RHVTVPLMTPVIFYSLILGVVEVMQYFLVPLVLTNGTGEPGGTTLFYNLYLYKQFFTF
                     QNMSYGATLAWVLFVITLAITLLVFWSARRWVYYAGER"
     gene            9445..10560
                     /locus_tag="VMZ33_03595"
     CDS             9445..10560
                     /locus_tag="VMZ33_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HUR16346.1"
                     /translation="MAAAALVPTVPPVPTAAAARPRRRPGPKDPVQGFIVTFVMVAIT
                     AAFLSPLVQAFTVSIKTPEQLSQARAPIWPATPRKFEYQGEQYDVYQVPIDGTVRSMA
                     LVEPRRTRSQFIDPANPDAGLVDWVGSWRTLEPAWEFDPQWGNYAKVWDVIDFPKVLF
                     NTFALAILGTVGTLVSCTLVAYGFARFRFPGRDSLFMLLIATVFLPGAVTLIPTYMIW
                     VKSGIMTSDIPFLPWAPLLIPTFLANAYDVFLLRQFLMTIPRDLDDAAAIDGAGPLRT
                     LVSVVLPQAWPAVVAVGIFHFVYTWNDFFGPLIYLSTHMDLQPVSVALSRFSGVYYTN
                     PAFIQAGTLMTLFIPVILFVVFQRIFTRGIVLTGVEK"
     gene            10557..>10898
                     /locus_tag="VMZ33_03600"
     CDS             10557..>10898
                     /locus_tag="VMZ33_03600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUR16347.1"
                     /translation="MTELRVALTFDAEHPDRPTRPGVLESILETLNVKSVRATFFMQG
                     RWVEAYPDTAAQIARAGHLIGNHSFYHARMPLLTDRGFAEDVAAAERVIKRACGVDPR
                     PWFRCPFGAGAD"
BASE COUNT         1773 a         3681 c         3507 g         1937 t
ORIGIN      
        1 cggtggcctg cgcatggcgt gtgaggcgat cgccgatgtg ctcggccagc agcagcatca
       61 ctcccgtctg gaagtcgacg ccgacctcaa gccagtgatc cggacgatca gtcaggacat
      121 ggctcgtgcg cggatggtcg acgcgcagca ggattgcctc tcgtccggcg atgatctgcg
      181 tcccacgtcg gctgatcacg ccggtggcga gcacgttcct gcagtagccg tgcgggtgaa
      241 cgaaggtgtc ggccagcgtc tcagcgggca gcacggtggt cggcaagtac acgcgcgaac
      301 ggcccgggag acgaggatcg tcgctcccaa cgggccgcag cggcactcgg cgattcgtgg
      361 ccgtcttggc acgcgcgtcg aacgtgcgca cagtcgtgcc gtcagtcatc caggcgtcgt
      421 actcacgagc gttgcccgtt tgttcgccgt aggtggtgat gaccttggcg ctgccggggt
      481 gacggaccca gacctcgtgt gtctcgagct cctccccgtg agtcgtgtag cgacgatcga
      541 cgatccgcat gcgcagtgaa tcgaaccgga gctctgcctc cgccatgaag gcgaacaggt
      601 cgttgacctc tgccgcggtg gtggccaggg tctgttgttc gggcggccgg agcgctggca
      661 gctgcggtgc tgaaggtgcg tcgtgcatcg cggccgattg tagacaggcg aactcgcgcg
      721 agccccaatg cccgccccgc ctaaacttca tccccgtgac ccagacacga accgaaaaag
      781 acccactcgg cccgctcaac gtccccgccg acgcctactt cggcgtccag acccagcgag
      841 cgatcaacaa cttcccgatc agcggacgtc ggccggacat cgcgctcgta cgggcggcag
      901 tccaggtcaa gaaggccgct gcacgcgcca acaaccagac gggacgacta cccgacgagc
      961 tggccaacgc gattgttcgg gccgccgatg aaattctcgg gctcacgtcg accgaggatg
     1021 gcctgcagca gacccttgtc gataacttcc gcgtcgatcc attccaggct ggcgctggcg
     1081 tgagccacaa catgaatacc aacgaggtgc tggcgaaccg agcgatcgag atcctgggct
     1141 cgcagggtgt cggcggcggg caacgcggtg actactcggt cgtcagcccc aacgaccacg
     1201 tcaacatggc ccagtcgacg aacgacacct tccccacgtc catgcgcatc gcgaccctgg
     1261 accgtgtcat ggagcttctg cctgtcctcg acgagctggc agccgccttc gctgaccgcg
     1321 ccgtcgcgtt cgatggcgtg ttgaaatccg gtcgcactca catgcaggac gcggtgccca
     1381 tccggctcgg ccaggagttc gccgcgtacg cgctgacaac ccgccgagca cgtgcggcta
     1441 tcgccacggc agccgacggc ctgctcgagc agaacatcgg cgcaaccgcc gtcggtaccg
     1501 ggctgaacgc agaacctgag tacatcgctc tcgtcgtgga gctgttgtct gagcagaccg
     1561 gccacgaact tcgacccgcc gaggacctgg ttcatgcgac gcagtcgatg gcgccgatgc
     1621 tggccgtctc tgctgcgttg cgcggcctgg ccgtggatct cgcaaagatc tgcgaggacc
     1681 tgctgctgat gtcgtcgggg ccgcggacgg gtttcgcgga gatcaggctg ccggcgcgcc
     1741 aaccgggatc gtccatcatg cccggcaagg tcaacccggt gatggtcgag aacctgtcga
     1801 tgatcagcta ccacgtcatt ggcgccgaca cggccgtggc ctgggcggcg tcgcgtggcc
     1861 agctggagct caacgtgatg atgccgctga tggcgcacga gctgctcgag tcgctgggca
     1921 tcctcaccaa cggttgccgc caatttgcac gcgagtgcat cgccgggatc gaggccgacg
     1981 tggaccgcgc cgcgtggctg ctggagcagt catctgccat ggccacgcca ctggcgccgt
     2041 acatcgggta tgccctcgcc gccgacatcg ccaaggaggc cgtgaagaca ggcaagacga
     2101 tccgtcagct ggttatcgag aagggcgtct tcaccgaggc tgaggtcaac cagatcctgg
     2161 acccgcatga gctgacccag ccgggtatcg ccggtggctt caaattcgag ccacggatgc
     2221 ctgagggcta caagaggccg actggaccgg tcggcggcgg aggctgacgc gaccctgttc
     2281 gacgacgagc ccgtcgcagt cgtggaaacc ctcgccatcg atgtcgtgcc cgcggccgag
     2341 gtcgacattc agttcgtcgt tcagcgcttc gatgcctagc gccgcgtcga gtccgttcgt
     2401 ccggcgcgat cgccgcgctc gcgagacaca ggaagaaggt ctcgctcccg cggcgacacg
     2461 ctcggcgaac agccacggcc gccggaggca cgaggagccg gacgagtacc gcacgtcgtt
     2521 tgagcgcgac cgtgatcgca tcctgcactc gaaggcgttt cgccgtctca agcacaagac
     2581 ccaggtgttc atcaaccccg acggcgatca cttcatcacc cggctgtcgc acacgctgca
     2641 ggtgacgcag atctcaaggg cgctggccgc ggcgctcacc ttgaacgaga cgcttgccga
     2701 ggccatcgcg ctgggtcatg acgtcgggca ctccccgttt ggccataccg gggaagaggc
     2761 actctcgccg tactttccgg agagtgagtg gcaccacgct gcccagagcg tgcgcatatt
     2821 cgacgtgctt gaggacctca acctcacgtg ggaggtcggt gatggcatcc gggcgcactc
     2881 gtggaagatc gagccgccgc cttcgacccc ggaggcgatg gcggttcgtt atggcgatcg
     2941 gatcgcgtac ctgacgcacg acgcactcga cgcgatgcgc ggcgggctcc tggccgcgga
     3001 cgacatgcca gcgcgcgctc tggatcgctt cgggccgccc ggccgcgtgt gggtcgacga
     3061 gatgatcaac gccgttatcg accacagcct ggccaccggc gaggtacgga tggacgaccc
     3121 gacgctcgag gtcatgaacg agctgcgcga cttcatgttc gagcgcgtct acctgtccga
     3181 agctcagcga ctccacaccc agaacgcgat agcggtgatc cggcgcctgg tcgactacca
     3241 cctggagcac cccgatcagc tgcccgattc ataccgggac acgggcgccg acctgacgac
     3301 ccaggtggtc gacttcgtcg ccgggatgac cgatagatac gccctgcagg ttcacgagcg
     3361 tctgttcggc ctcgacggtt aggcgtcagt tcgggcagcg ccgcggctcg tccgttcgac
     3421 cggcggcacc agcaaccaaa ttcgacccgt tgccgcggtc ggcgccgcat caaatcccgg
     3481 tcgctagcat cctcggctcg tgtccaagcc tccgacgccc tccgggcatg cgacgccctc
     3541 gcgccgcagc gcggttgtcg ccttcatctg cgtattcagc gctgtcggcg cgagcgcgcc
     3601 gtttctgcct gtgtactacc agtcgctcgg gctttcgctc gacgccatcg ggttgctcgc
     3661 tgcggtcgca gcgctgacgg cgttgttcgc ggcaccggcc tggggcgtcg tcagcgaccg
     3721 gctccgctcg cggtacgtcc tcctggtcgc ttgcctcggc gcggcgatca gcagcgtttt
     3781 gctggggctg gccgtcgagc cggtcgtggt cgcgctgtgc gccatcctgt actggctgtg
     3841 gttcgccggc gttccgccgc tcttggacgc gcaggcgctc gaggccgtgg gcgaagacca
     3901 cttccaatac agccggctgc gcgtttgggg ctcagccgcg ttcatcgtga gcacgttggt
     3961 ggtaggcgcc ctgatcgaac gtgccggcct gcgcagcctc ttcgtggtct tcgtcggcgc
     4021 cctcatgttg accggcctcg ttgcgctgcg cctttccacc acacacgcgc ggccgagcat
     4081 gccgcgcctg agcggcctgc gggctgtact cggcaaccgg accgccgtga ccttcatcgg
     4141 catcgcacta ctgacctgga gtgcctcaac cgccgtcaac ggcttcctgt cgatctacat
     4201 gatcgagata ggcactccgg ggactgtcat agggttcgcg tgggccctcg gcgccggcgt
     4261 cgagatcccg ctgatgatcg cgtttccgca gctcgcgcgg aggttcggcc tcgagacact
     4321 cgtcgtggcc ggcgccgcat tcctgctgct acgcgtgctc gccttgctcc ttgttcacga
     4381 cccgctcctc gtggcagcaa cgatgacgct ccacggcgcc ggcttcgccc tgctgctggt
     4441 cggcggcgtg acgtacgtcg ctcgacatgc cccgtcggga gcggaggcga cggcgcaggg
     4501 gttgctgtcg ggcgtgacta tcggcctttc ccaggcggtc gggccgctct tcggtggggc
     4561 actcgccgga gtagttgggc tgccgacgat gtttgtcttc ccggccgttg gaagcgccgt
     4621 tgcgctcgcc gcaatggttt gggcggtgct tcggcccgcg gtcagcgagc gaagagcggc
     4681 aggtagcgct cgttgacctg caggagctcc tccaggagcg gggacgcgac gtcgtagccg
     4741 ccgacgagcg ggttggagag caatgcccgc agggccacgt cgcggtcgcc ggtcgtcgcc
     4801 gcctggacgg tgaggcgttc gtagcccttg gcgtggttca cgagcgccag catgtccgtg
     4861 gtcagagccg cagccggcag ggtgcgcggg ccttcgcgcg tgatcgtggc cggcacctcg
     4921 acgacggcgt cgtcaggcag ctccgggatg gcgccgtcgt tacgtgtgtc gaccacctgg
     4981 atgtcgccac ggccatcgtg gagagacgcc atcagctgcg cagccgcctc gctgtagaaa
     5041 gcgccgccgc ggttctccag cagcgccggc ttctgggtca gcgacgggtc gcggtacatc
     5101 tccagcagcc gtgcctcgat gtcgagaacc tcacgagcgc gcgagtgctc gccagattcc
     5161 tgctcgcgga ggacgtcgtt gaagcagtag tagtagcgca ggtacgacga gggaacagcg
     5221 cccaacaccc ggacgagctc ctgcggaatc ccgccggcct cgttagcaag gtcagtcagg
     5281 ttcccgtcga ggagttcagg taaccggtcg atgtcgtcga cccagacgtg gcggacccac
     5341 gagaggtgat tgagacccac gtgtcccagc cgaacccgct caggctccac accgaaacgc
     5401 gacgccatac ggcgctgaag accgattgcc acgttgcaca gtccgatagc ccgatggccg
     5461 gcgtcaagca gcgcctgggt cacgatgccc accgggttgg tgaagtccac cagccatgcg
     5521 ccgggcgcag cgcgccggcc ggtcaattcg gccaactcga ggactactgg aacggtccga
     5581 agtgccttcg cgaagccacc tgcaccggtg gtttcctggc cgatcgttcc gtatcggacg
     5641 gggatcgtct cgtcggtgag tcgtgcctgc tggccgccga tgcggagctg cacgagcacg
     5701 aaggacgcgc cgtcgacggc ttcctcgtgc gaatccgtcg ccgtgatcac gcccgcccat
     5761 ccgagccgtt ccatgatccg acgcgcgaac ccggcgacga cttcgaggcg ctccgggttg
     5821 acgtccatca gcgccagctc agcgaccggc agacgatcgt ggcgccgggc gatgccttcg
     5881 atcagctccg gggtgtatgt gctgccacca ccgaccaccg ccaccttgac cgatccagag
     5941 gtagttgaca ttgcttataa cgttatcagc aattccggcg gtcgcaagcc ggaacgatgc
     6001 cgatcggcag gcttgcggag cagtcgagga gacgcgtggc caaagcacgg acgctgctca
     6061 ggtcgggtcc ggcaacgctc catcaccagg tgtatgtcga cctgaagacg gccatcgaag
     6121 aaggccgcta cgcacacggt gaccggcttc cccccgaacg cgatctggcc cagcaatacg
     6181 gctgcagcct gatcaccgtc cggcgcgccc tggctgaact cgcgcgcgag caacgaatcg
     6241 agcgcaccca gggacgcggc acgttcgttc ttcgaccccg gatagatcgc gaccttggcg
     6301 acaccaacag cttcaacgag gagatggcgc ggctcggcct gataccggaa acgcgcgtgc
     6361 tgtccgcacg atcggaagcg gccggggagt cggtggcgaa ggcgctggag cttcaaccgg
     6421 gctcgccaac gcttcatctc gagcgcctgc ggctcgcgtc gggtgagccg ctgctgctgg
     6481 agatcgtttc tctgccagcg gagagatttc cggggctgct tgcctccgac ctcgagcgcg
     6541 gctctctcta tgaactgctg agccagcgct atggcaccga gatcaccagt gcccgcgaga
     6601 cgctggagcc cgttctcctg cggatgcgcg aggcgcgact cctgggtcag aagacccgca
     6661 cccctgcgct gctcgtcgag ggccttgcct acacgtcgtc gaacgtgccc gtcgagttca
     6721 gccgcaccta tgtcagaggc gatcgaaccc gctactacgt acaacgcgtt gttgtgcgtg
     6781 agggggcgcg gccaagcgac cgcccaacgc agccggctcg tggccggggt cagcaagtag
     6841 ccgcggccac ttcggcacgc ggctaaccat ggaggaggag atggccagac gaaccggggc
     6901 atcccgtatc ggggtgcttt tcgccgttgt cgtgctcacg cttggcgcgt gcagcggaca
     6961 gacggctaca cagcgaccat cgacaggctc acccggcgct tcaacgcctg cacagaccgc
     7021 ctcggcggcc gcctcggagc ccgcgccgag ctctgatgcc agcgctgagc cgagcgccga
     7081 accgagcgcc gaaccgagcg ccgaaccgac gagtgacgtc acggcagccc cgtccttcga
     7141 accgggaagc ggtgacatcc gctggttctg ctgcctgggt accggcgagg acccggaggc
     7201 gcagattccg gttgagatgg ccgccgcagc ggatttcagc accgagcatc ccgacacctc
     7261 catgaccttc gaggtcatca cgtacgacgc cgcccgcgac accctgtcga cgatgatcgc
     7321 tggccagaac gctccggatc tcgtagggcc ggccggcgtc ggcggcattg aggcttacca
     7381 cgggcagtgg ctggacctga ccccgtacat cgaagccgct gagttcgatc tcacggctta
     7441 tcccgaagcg ttggtcaact tctacaagca cgatgacggc cagatcggta ttcctttcgc
     7501 ggtctacccg tcgatgctct ggtacaaccg cgacctgttc gacgaggcgg agctggatta
     7561 cccgccccac gaatatggcg cgccgtacgt catgcccgac ggcaccgagc gcccgtggga
     7621 ctacgacacc gtccgcgagc tggcgctgtt cctgacggtc gacgccaacg gcaacgacgc
     7681 gacgtcagcc gagttcgatc cggaaaacat cgtccagtgg ggcttcgagc cgcagcgcga
     7741 cctgctcgcc tcacttggtg cttacttcgg cgccggcagc ctcgaggcgt cgggcggtgc
     7801 ggcccaagtg cccgatgctt ggcgtgacgc gtggaagtac ttctacgacg gcatgtggac
     7861 ggaccgcttc atcatgaccg gaccgcagtt cgacgacatc gcgccagatg ccggcggata
     7921 tgcgttcttc tcggggcgcg tcgcgatgtc aaccaacttc ctgtggacga cgtacggcgt
     7981 cggcgaagac tcgggcgtgg caggcgtctg ggatctcgcg gccgttccct cgcacaacgg
     8041 aaccacgacg tcgccgctga atgcggacac cttcgtgatc cacgagtcca ccgacaaccc
     8101 ggaggccgct ttcgacgccc tgacgtacct acaggtggat aggggagcgg agctgctcga
     8161 catctacggc gggctgccgg cacgaacgga agaacaagac gcattcctgg ctggccaggc
     8221 cgaggcgttc ccgcacgacg tggattggca ggttgtcaag gacagcctgg cctacccaga
     8281 taacccgaac tttgaagcgt tcaagcccgc atacagcgag acagacgcca tcctcgggac
     8341 gtacctgacg agatggacga caacgccagg gctcgacctt gacactgaga ttgccgcgct
     8401 cgaggcggaa atccaggctg cctgggacgc caacccttaa tcgcgtagat caagcggcgg
     8461 agcagagcac taacaggtga cgacgatcag ccgcgcccgt agtgctctgc gccgtcccaa
     8521 gatgacgccc ctggcgcgcc gagaagcccg ccaggggctt ctcttcatct cgccgtggct
     8581 cctgggcttc gtcctgttca cggtcttccc gatgctggcg acgctgttct tctcggtgac
     8641 caacatccag ctcaaccagg aagagcccct gcggtttgtg ggattcgata actactcgcg
     8701 cttcctggac gacaagcagg cgatcggttc gctgcgagtc accctcagct ttgccgtcct
     8761 gtggctgcct gtctcgatcg tcgcgccctt tctcatcgcc cttctgctga acagtgtcca
     8821 cctcgtcggc cggggcgtgt tccgcgtgct gctcttcatg ccgtacgtgg tgccgttcgt
     8881 ggccggcgcg ctcatctgga acagcatgct cgggctcaac gggtggatca acgagttcat
     8941 gcgcatgttc ggcttcgaca tagacacgcc atggctgcgt gacccgtcgc tcgtgtaccc
     9001 cgggctggcc ctggtcggcg tgtggggcat cggcggcgca gtcatcatca atctcgctgg
     9061 cctgcgcggc atcccgaccg agctgtacga cgccgccaag atcgacggcg cgggcggctg
     9121 ggcgcagctc cgacacgtga ccgtgccgtt gatgacgccg gtcatcttct actcgttgat
     9181 cctcggcgtc gtcgaggtca tgcagtactt cctcgtgcca ctcgtgctca ccaacggcac
     9241 cggcgagccc ggcggaacga cgctcttcta caacctctat ctgtacaagc agttcttcac
     9301 gtttcagaac atgtcctacg gggcgacgct ggcgtgggtc ctcttcgtca tcaccctggc
     9361 gatcaccctc ctcgtgttct ggtccgcacg gcggtgggtc tactacgccg gcgagcgcta
     9421 gcaatgaccc aggtggatca accgatggcc gccgccgctc tcgtgcccac cgtgcccccc
     9481 gtgcctacgg cggctgcggc ccggccacgc cgccggcccg ggccgaagga tcccgtgcag
     9541 gggttcatcg tcacttttgt catggtcgcg atcacggccg cgttcctgtc gccgctcgta
     9601 caggccttca ccgtttcgat caagacgccc gagcagctca gccaggcgcg ggcgccgatc
     9661 tggcccgcga cgcctcggaa gttcgaatac cagggcgagc agtacgacgt ctaccaggtg
     9721 ccgatcgacg gcacggtgcg gagcatggcg ctcgtcgagc cgcgccgaac ccggagccag
     9781 ttcatcgatc cggcaaaccc ggacgccggg ttggtcgact gggtcggctc gtggcgcacg
     9841 ctcgagccgg cctgggaatt cgacccgcag tggggcaact acgccaaggt ctgggatgtg
     9901 atcgatttcc cgaaggtgct cttcaataca ttcgcactcg caatcctcgg caccgtcggg
     9961 acgctcgtgt cgtgcacgct cgttgcgtac gggttcgcgc gcttccgttt cccgggtcgc
    10021 gactcgctct tcatgctcct gatcgccacc gtcttcttac ccggggcggt caccttgatc
    10081 ccgacgtaca tgatctgggt caagtcgggg atcatgacct ccgatatccc cttcctgccc
    10141 tgggcgccac tcctgatccc aaccttcctc gcgaatgcct acgacgtgtt ccttctgcgc
    10201 cagttcttga tgaccatccc gcgtgatctc gacgacgctg cggcgatcga cggcgccggc
    10261 cccttgagga cgctcgtctc agtcgttctc ccgcaggcgt ggccggccgt agtcgccgtg
    10321 ggcatttttc acttcgttta cacgtggaac gacttcttcg gcccgctcat ctacctgtcg
    10381 acgcacatgg acctgcagcc cgtgtcggtc gcgctttccc ggttttcggg ggtgtactac
    10441 accaaccctg ccttcattca ggcggggacg ttgatgacgt tgttcatccc ggtgatcctg
    10501 ttcgtcgtgt tccagcggat cttcacgcgt ggcatcgtcc tgaccggcgt cgagaagtga
    10561 cggagctgcg cgtcgcgctc accttcgatg ccgagcaccc cgaccgccca acgcgcccag
    10621 gggtgctcga gtcgatcctc gaaacgctaa acgtgaagag cgtgcgggcg acgttcttca
    10681 tgcagggtcg atgggtggag gcgtatcccg acactgccgc gcagatcgcg cgggcaggcc
    10741 acctgatcgg aaaccattcc ttctatcacg ctcgcatgcc gctcctgacg gaccgtggct
    10801 tcgccgagga cgtcgcagcc gcggagcgcg tcatcaagcg tgcgtgcggg gtcgatccgc
    10861 gtccctggtt ccgctgccca ttcggtgccg gtgctgac
//
LOCUS       DATLCO010000144         4398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3912175, whole genome shotgun sequence.
ACCESSION   DATLCO010000144 DATLCO010000000
VERSION     DATLCO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3912175"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VMZ33_03605"
     CDS             <1..180
                     /locus_tag="VMZ33_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16348.1"
                     /translation="QDVVLPVPSGKQARQAADTYIDQPTDTQFEPPAPVPQIASSAAE
                     NRVPDRAPRAAPTEN"
     gene            300..1184
                     /locus_tag="VMZ33_03610"
     CDS             300..1184
                     /locus_tag="VMZ33_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HUR16349.1"
                     /translation="MGVGGFVVLLIAAGAPPPWGGLLFMAALLATTLGLGAAAGYQLI
                     ARVTRPASAFRGPSPAILLALQLIVSIALGTAWVVLGVPDPQSSSAGFLIIAATLLVT
                     YVGIVWLFVVRSGVLSWSEMGWPTRAPASRLLGDVGVGFAAMLVAWPVVTFLAALLGT
                     LLQSTPTNPVPSISTTTDTLLTAIGAALLVPIGEEVLFRGYALTAWLRDLGPRSALIR
                     STLFFAFAHILGVTAASFDEGWRQALLTVVVITPVGAVLGILFLRRGLLAAIAGHVTF
                     NLISVLAMALAQSLLPPQ"
     gene            1362..2012
                     /gene="radC"
                     /locus_tag="VMZ33_03615"
     CDS             1362..2012
                     /gene="radC"
                     /locus_tag="VMZ33_03615"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HUR16350.1"
                     /translation="MSDARADSRHAIRELGPSSLSSADLLEVICGADVGSTLLARHGS
                     LSELARADFVELRTAPGVGAARAAQIAAAFELGRRAATPSLAGRWVIRSPRDVADRLI
                     PEMAHLEREELRVLLLNTKNAVIRAVTVYQGNVSAAVVRVAELFRDAVRVHAAGIVMV
                     HNHPSGEAEPSADDLHLTAEAVAAGRLLDIALLDHVILAADSYVSLRDRGVAFDRR"
     gene            2160..3212
                     /locus_tag="VMZ33_03620"
     CDS             2160..3212
                     /locus_tag="VMZ33_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HUR16351.1"
                     /translation="MLDAFLGMFSRDIGIDLGTANTLVHVKGRGIVISEPSVVAIEAR
                     SKRVLAIGAEAKRMLGRTPANIIAVRPLRDGVISDFDVTEQMIKYFVRKVHDRVGMIP
                     RPRMLIGIPSGVTEVEKRAVRDAAINAGARWARLIEEPMAAAIGAGLPVGEPTGSLIV
                     DIGGGTTEVAVISLGGIVVSRSLRIGGDELDQDIITYARREYNLLLGERTAEDIKIAI
                     GSAYPGEYDDQRVTLRGRDLLTGLPRAVEITAEQVREALEASVQQIVDTVKDTIEETP
                     PELVADIMDQGIVLAGGGALLQGLDRRVSEATQMPVHVADDPLTCVARGTGKALEELD
                     LLERALVAETYSHAPR"
     gene            3264..4220
                     /gene="mreC"
                     /locus_tag="VMZ33_03625"
     CDS             3264..4220
                     /gene="mreC"
                     /locus_tag="VMZ33_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HUR16352.1"
                     /translation="MSRAARRQTALYVFLVAFTLILVAFSNSGPLLELRRGVGFALSP
                     IQDTLRQGTRTVTSLFATIGEIEELRQTNEELSQRLQAVEADNQRLLSLAAENEQLAD
                     LLGVRSSIGYETVASEVIGRRTTEQERVISIDAGTDVGVNVDDPVVAGGGALIGRVVE
                     VGPNFARILLINDTRMNVAGLVETTRAIGDVQGNGERPLQMTRIPATDTVNVGDAVVT
                     AGIELEGGIRSTYPKGLLIGTIASIQRQPDQLFQTALVQPVAGLDRLEYVLVIINYEG
                     GPSAESPLPSGSAAASPVGSPAVTVAPPTAVPTAFPTLAPTT"
     gene            complement(4217..>4398)
                     /locus_tag="VMZ33_03630"
     CDS             complement(4217..>4398)
                     /locus_tag="VMZ33_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HUR16353.1"
                     /translation="VRRGEYLSYIAGLYCTTVAEILELNQIEDPDVVQPRTALLIPGG
                     GCPPEANAPPAQAPA"
BASE COUNT          682 a         1486 c         1447 g          783 t
ORIGIN      
        1 caggatgtcg tcctgccggt tccgtcgggc aagcaggcgc gccaggcggc tgacacctac
       61 atcgaccagc cgaccgatac gcaattcgag ccgccggctc ccgtgccgca gatcgcctcg
      121 agtgcagccg aaaaccgggt ccctgaccga gcgccgcggg ccgctccgac cgagaactga
      181 cgtccgcaat ttccatcgag cgcccggacg ccagccccgc tgccggccct gctgattcac
      241 cgggcagctt ctccgtaggg ggtcggcccg ctgccggtct ctatcttctc gcctggcttt
      301 tgggggtcgg tggcttcgtc gtgttgctga tcgccgccgg cgcgcctccg ccgtggggcg
      361 gtctgttgtt catggcagcg ctgctggcga ccacgctcgg actcggagcg gcagcgggct
      421 accaactcat cgccagggtc acgcgacctg cctcggcatt ccgtggcccg tcaccggcca
      481 tcctgctcgc cctccagctc atcgtgtcga tcgcactggg aacggcatgg gtcgttctcg
      541 gagtcccaga tccgcagtcc agctcggccg ggtttttgat catcgccgcg acgctgctcg
      601 tgacgtatgt gggcatcgtc tggctcttcg tcgttcgttc cggcgtgctt tcgtggagcg
      661 agatgggctg gccgacccgt gcgcctgcct cacggctgct gggcgacgta ggcgtcggct
      721 ttgcggcgat gctggtggcc tggccggtgg tcacgttcct ggccgcgctg ctcgggacgt
      781 tgctccagtc gacgccgacc aaccccgttc catcgatatc gacgacaacc gacacgctct
      841 tgaccgcgat tggtgccgcc ctgctcgtgc cgattggcga agaagtgctg ttccgcggct
      901 acgcgctgac ggcgtggctg cgcgatctcg gcccccgctc cgccttgatt cgcagcacgc
      961 ttttcttcgc attcgcccac atcctcgggg tgactgcggc ctcgttcgac gaaggctggc
     1021 ggcaggcact gttgacggtg gtggtgataa ctccagtggg cgccgtcctg ggcatcctat
     1081 ttctgcgccg aggtctgctc gcggcgatcg caggccacgt cacattcaac ctcatcagcg
     1141 tgctcgcgat ggcgctcgcc cagtcccttc tgccgcccca ataggacctg cggacagagc
     1201 agaactgtcc ggtgagccgg tgttgatcgg cgggctacgg cgggtgaagg gccggttcct
     1261 agagttcacg tcgctccctt gttcggatgg cggggaaatc ggcgggctcc gttgtccgga
     1321 cctgggagcg tttcaccagc actacctgag gaacaaatcc cgtgtccgat gccagggcgg
     1381 atagccgaca tgccatccgc gagcttggcc cgagcagtct gtcgtcagcg gacctgctcg
     1441 aggtcatctg cggcgcagac gtcgggtcga ctctcctcgc tcgacacggt tcgctctccg
     1501 agctggctcg cgctgatttc gtggagctgc gcacggcgcc cggagtcggc gcggcacgcg
     1561 cggcgcagat cgcggccgcg ttcgagcttg gccgccgcgc tgcgacaccc tccttggccg
     1621 ggcgctgggt gatccgctcg ccgcgcgacg tcgccgatcg cctgattccc gagatggcgc
     1681 acctggaacg cgaggagtta cgggtcctgt tgctcaacac gaagaatgcc gtgatccgcg
     1741 cagtgactgt ctatcagggc aacgtctcgg cggccgttgt tcgcgtcgcc gagttgttcc
     1801 gcgacgcggt tcgagtccac gccgcgggca tcgtcatggt gcacaaccac ccgagcggcg
     1861 aggctgagcc gtcagccgac gaccttcatc tcaccgctga ggcagtcgcc gccggtcgcc
     1921 tgctcgacat cgcgttgctt gaccatgtca tcctcgccgc ggactcgtat gtcagcctgc
     1981 gcgatcgggg agtggccttc gaccgccgat agccgcgcta accggcgtcg ggctgtcgct
     2041 cggaggcacc acgatcggcg gtgggccgag gcgggtatac actgctcaac cttcgtcgcg
     2101 cgcctatccg ccgtaaacgc cgggccgcag acgctccaca cctggggatc atcatcggaa
     2161 tgctcgacgc ctttctaggc atgttctcgc gcgatattgg catcgacctc ggtacggcca
     2221 acacgctcgt ccacgtcaaa gggcgcggca tcgtcatcag cgagccctcg gtcgtggcga
     2281 tcgaggcgcg gtcaaagcgc gtgttggcca tcggcgccga agccaagcgc atgctcggcc
     2341 gaactccggc caacatcatc gccgtgcggc cgctgcgaga cggcgtgatc agcgacttcg
     2401 atgtgaccga gcagatgatc aagtacttcg tgcgcaaggt gcacgaccga gtcggcatga
     2461 tcccgcgtcc gcggatgctc atcggcattc catcgggagt gaccgaagtc gagaagcgcg
     2521 ccgtgcgtga tgcggcaatc aacgctgggg cacgttgggc gcggctcatc gaggaaccca
     2581 tggcagcggc gatcggtgcc ggcttgccag ttggcgagcc caccgggtcg ctcatcgtcg
     2641 acattggcgg cggcaccacg gaagttgccg tcatctcgct gggtggcatc gttgtgagcc
     2701 gcagcctgcg tatcggtggc gatgagctcg accaggacat catcacctac gcccgacgtg
     2761 agtacaacct gctcctcggc gagcggaccg cagaggacat caagatcgcg atcggctcgg
     2821 cctacccagg ggagtacgac gaccagcgcg tgaccctccg tggccgcgac ctgctgactg
     2881 gtctgccacg ggcggtcgag atcacggctg agcaggtccg cgaggcgctg gaggcgagcg
     2941 tccagcagat cgtcgacacg gtcaaggaca cgatcgagga gacgccaccg gagctcgtcg
     3001 cagacatcat ggaccagggc atcgtcctgg ccggtggcgg ggcgttgttg caaggcctcg
     3061 ataggcgtgt aagtgaggca acccagatgc cggtgcacgt cgccgacgat ccgctcacgt
     3121 gcgtggcccg cggcacgggc aaggccctcg aggagctcga ccttctcgag cgggcacttg
     3181 tcgccgagac ctactcgcac gcgccgcgct aagggcgact gagccgcacc cactcgccga
     3241 ccgcgagacg tgactgagca cccttgagcc gagccgcgcg ccgccagacg gcgctctacg
     3301 tttttctcgt cgccttcacg ctgatcctcg tcgcgttcag caatagcggg ccccttcttg
     3361 aactgcgccg cggcgtgggt ttcgcgctct cacccatcca ggacaccctg cgccagggaa
     3421 cgcgcaccgt gacgtcgctc tttgccacga tcggtgagat cgaggaactg cgccagacga
     3481 acgaggagct gagtcagcgc ctccaagccg tcgaggccga caaccagcgt ttgctcagcc
     3541 tcgccgccga gaacgagcag ctggccgacc tgctcggcgt gcgcagttcg attggctacg
     3601 agacggtcgc gtcagaagtg atcggccggc gaacgactga gcaggagcgc gtcataagca
     3661 tcgatgccgg caccgacgtg ggggtcaacg tcgacgatcc cgttgtggcc ggtggcggcg
     3721 cattgatcgg ccgtgtcgtg gaagtcggcc ccaacttcgc gcgcatcctg cttatcaacg
     3781 acacccgaat gaacgtcgcc gggcttgttg agacgacacg cgccatcggc gacgtacagg
     3841 ggaacgggga gcgaccactg cagatgacgc gcattccggc cacggacacg gtcaatgtcg
     3901 gcgacgcagt ggtgaccgca gggatcgagc tcgagggtgg gattcggtcg acctacccga
     3961 agggccttct catcggcacc atcgcctcca tccagcgcca gcccgatcag ctcttccaga
     4021 cggccctggt gcagccggtg gccggtctgg atcgactcga gtacgtactc gtcatcatca
     4081 attacgaggg cggtccctcc gccgaatcgc cattgccgag tgggtccgca gcggcctcgc
     4141 cggtcggctc gcccgcggtc acggttgcgc caccgactgc agtcccgacc gcattcccga
     4201 cgctcgcgcc gactacctag gcgggcgcct gggctggcgg tgcgtttgcc tcgggcggac
     4261 atccgccgcc cggtatcagg agcgccgtcc gtggctggac gacgtccggg tcctcaatct
     4321 ggttgagctc caggatttcg gccacggtcg tgcagtacaa accggcgatg tagctcaggt
     4381 actcgccgcg tcggacga
//
LOCUS       DATLCO010000145         5918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1681088, whole genome shotgun sequence.
ACCESSION   DATLCO010000145 DATLCO010000000
VERSION     DATLCO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681088"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..2006
                     /locus_tag="VMZ33_03635"
     CDS             438..2006
                     /locus_tag="VMZ33_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HUR16354.1"
                     /translation="MERLRERRDRLAVRQLDLVLVLTAAGLLLLTAAFLFDPSLSFAL
                     VDRAADTVVHSLTALAAASLAALALARYRESGRIAGLFQASAFLLMALVTIVVVAVII
                     AKREADVGLTLGRPEQLPVYISSVTRVVAGAILLVGGAAGIGMVTHAPRVRRTLLAPF
                     VAISALTVVLYTIRELFTNFDEIIPPFIGDEGIQRLLEDPTIGGPLVDVSGIALLIQS
                     VAAALFLAGAFLYRRSYLRDGPVADGFLAVAMLIGFFSEIHFYFYPAVYGGLVTTSEV
                     LRLAFFGVLLFGINAESRADLRALRSAYAALDRLRLSEADRATLEERTRLARELHDGL
                     AQDLWFAKLKHERLAVHVPEEHQALAAEVTQAIDAAIAEAKQAVMTMRSSEERDISLE
                     ELISRSVDDFASRSGVRADLSVADLPTELPARTQAEMLRVLQEALTNVRRHADATVVR
                     VNAEVADGWFVLRIVDNGRGFRPEETSGEGLGVQGMKERARLMGGDLRVMSEPAGGTA
                     IQLMVPMGGTVAKS"
     gene            2031..2693
                     /locus_tag="VMZ33_03640"
     CDS             2031..2693
                     /locus_tag="VMZ33_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16355.1"
                     /translation="MSEIDGKLRVMLVDDHALVRSAVRQALTAPDVEFVGEAANAQEA
                     LALAPMLRPDVLLLDIDLPGMNGIQLVQELAPRMPQTKIVMLTVSGAERDMLEAVARG
                     ASGYLTKDLSPEALLRSLRGTQRGELAMSRRFAARALRYFADAARRGKSVSGQETELM
                     GLSPRENDVLAMLADGLTDREIANALTISPRTVETHVSNILHKLGVRNRAEAAQRYRQ
                     ST"
     gene            complement(2897..3580)
                     /locus_tag="VMZ33_03645"
     CDS             complement(2897..3580)
                     /locus_tag="VMZ33_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HUR16356.1"
                     /translation="MDVLDRIDAMIDERHLLKHPFYTKWSEGTLPREALQEYARQYYA
                     FESSFPRYLSAIHTRTESAAARQQLLDNLWDEEHGEANHAELWLRFAEGIGVDRADVR
                     SADRNEATQELVDLYADLSTNATVPAAIAALYAYERQVPQTSMSKIDGLARHYDVEDK
                     RTTSFFAVHGVLDIEHSGAEREMLGSLIAADDDTDTTAIEDATQRALDAWWNFLSAVD
                     MPAMETAAA"
     gene            3629..4702
                     /locus_tag="VMZ33_03650"
     CDS             3629..4702
                     /locus_tag="VMZ33_03650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR16357.1"
                     /translation="MKRFDVGIIGGGIIGATAAAFLAEAGRSVLLLERGGIGAGASGR
                     NSGVIQHPFDPLFATLFYGSLDLYRELASQDDDFAIPDSPAGLLLLSKDGSAVVEATR
                     TIAESAPDLSPEVVDPSALATLEPALAEGLWACRLHTGYPVEPAGATHAWARRARRAG
                     ATFALGKEATVDMRNFDQVLIAAGPWTPALVPGWSDAAPIVPIWGVVVSTTLRDAPRH
                     VLEEMGIARSGETQEREFSLVTAGGRSSLGSTFLPHEPDAPALAPELIARGAQFVPEI
                     GRVSIDGVRACARPVSRDGRPLIGLVDGRDNLFVCAGHGPWGISTGPASALMVVRRML
                     GQAEVHPAFAARRFSGLTTTEFA"
     gene            5159..5413
                     /locus_tag="VMZ33_03655"
     CDS             5159..5413
                     /locus_tag="VMZ33_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16358.1"
                     /translation="MNGRVGSTAERCVPGSEFSPVKRFRRGLANTMPATTEPTRSSRI
                     VAKNSGLTSALTVVSAASLVAGATATAGIEKPKINAGACG"
     gene            5604..>5918
                     /locus_tag="VMZ33_03660"
     CDS             5604..>5918
                     /locus_tag="VMZ33_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16359.1"
                     /translation="MTLGTPAPAVADRLAEPPYRVRARLLTPLLNGGVTFHDDALLTV
                     DERGLIAAVEDARVGGDAGVDAFDLRPMVLMPGLIDLHAHLPQYPNAGLGGGLDLLTW
                     LER"
BASE COUNT          925 a         1893 c         2028 g         1072 t
ORIGIN      
        1 cgcggcaaga atcaggcccg cgagaatgtg gcgcgccgac gccgacagca gcggcggcat
       61 cgtctcgacg acgatacgga tgcccaggta cgtcgaaccc caaacgatgt acacgacgag
      121 caacgacgcc cagatctgcc agttgggtgc cccttcgtca gggcgtgcct gtttcatgtg
      181 cctcctgcgg ccgattgaac gtcaccgaaa ctcacgggaa cgccccttag ttgcactacg
      241 gagtacggat acagactccg tacacatttc cgtacgttct cggattcccg tgccagtgcg
      301 cgaccgccat tcttacccca caagaacatt tgagcttcgg cgccatcctt acacggcggg
      361 gtgtgctgac gaagtgcgac aacgagccgc atcggcgctg gggcgcgatg cggctctagt
      421 ctttctatca tcggaggatg gagcgtctgc gcgagcgtcg cgaccggctg gcggtccgcc
      481 agctcgacct agtcctcgtc ctgacggccg ccggcctgct gctgcttacg gctgccttcc
      541 tgttcgaccc ttcgctcagc tttgccctcg tcgatcgcgc ggccgacact gtcgtccact
      601 ccctgactgc actggctgcg gccagcctcg cggccctggc attggcgcgc taccgcgaat
      661 cgggtcgtat cgctggtctg ttccaggcga gcgccttcct gctcatggca ctggtcacga
      721 tcgtggtggt ggcggtgatc atcgccaagc gtgaggcgga cgtcgggctg acgctgggtc
      781 ggcccgagca gctgcccgtc tatatctcgt cggtcactcg agtcgtcgcc ggtgccatct
      841 tgttggttgg cggcgcggcc ggcatcggga tggtcaccca tgccccccgc gtgcggcgca
      901 cgctgctggc gcctttcgtg gcgatctccg cgctgacggt cgtcctgtac acgatccgcg
      961 agctgttcac caacttcgac gagatcatcc cgcctttcat cggcgatgag ggcatccagc
     1021 gactgcttga ggacccaacc attggtggcc cgctggtcga cgtgagcggc atcgccctgt
     1081 tgatccagtc cgtggccgca gccctgttcc tggcgggcgc ttttctctac cgacgttcgt
     1141 atctgcgcga cggccctgtg gccgacggtt tcttggccgt ggcgatgctc atcggcttct
     1201 tctctgaaat ccacttctac ttctacccgg ccgtgtacgg ggggctggtg acgacctctg
     1261 aggtgctccg gctggcgttc tttggagtcc tgctatttgg tatcaacgcc gagtcgcggg
     1321 cggacctccg agcgctgcga agcgcgtacg cggcgctcga ccgacttcgt ttatcggaag
     1381 ccgaccgggc tacgctcgag gagcgcacgc ggttggcgcg cgagctgcac gatggcctgg
     1441 cgcaggacct ctggttcgcg aagctcaagc acgagcggct ggcggtccac gtgccggagg
     1501 agcatcaggc gctggcggcg gaggtgaccc aggcgatcga tgccgccatc gccgaggcca
     1561 agcaggccgt gatgaccatg cgctcgtccg aggaacgaga catctcgctg gaggagctca
     1621 tctcgagatc cgtggacgat ttcgccagcc gatcgggcgt gagggccgat ctgtcggtgg
     1681 ccgacctgcc gacagaattg ccggcgcgga cacaggcaga gatgctgcgc gtcctccagg
     1741 aggcgctgac caatgtgcgc aggcacgcgg acgcgaccgt ggttcgggtg aacgcggagg
     1801 tcgccgatgg ctggttcgtc ttgaggatcg tcgacaacgg acgtggcttc cgacctgagg
     1861 aaaccagcgg tgaagggctc ggcgtgcagg gcatgaagga gcgtgcgcgt ctcatggggg
     1921 gcgacctacg cgtcatgtca gagccagcgg gcggcacagc catccaattg atggtgccca
     1981 tgggcggtac ggtggctaag tcatgattca agcgcgatga gggcagcggc atgagcgaga
     2041 tcgacggcaa gctgcgggtg atgctggtcg acgaccatgc actcgtccgt tccgccgtgc
     2101 gccaggccct gaccgcgcca gacgtcgagt tcgttggtga agcggcgaat gcccaggagg
     2161 cgctggcgct cgcgcctatg cttcggccgg acgtactgct gttggacatc gacctgccgg
     2221 ggatgaacgg gatccagctg gtgcaggaac tggccccgcg gatgccgcag acgaagatcg
     2281 tgatgctcac cgtgagtggc gcagagcgcg acatgcttga ggccgtcgcc cgtggcgcgt
     2341 cgggctacct gaccaaggac ctatccccgg aggcgctgct tcgctccttg cgtggtactc
     2401 agcgcggtga gctcgccatg tcacggcgat tcgccgcgcg tgcgctgcgt tacttcgccg
     2461 atgccgcgcg gcgcggcaag tccgtgtctg gacaggaaac cgagctgatg ggcttatcgc
     2521 cgcgcgaaaa cgacgtactg gcgatgctcg ccgacggcct caccgacagg gagattgcca
     2581 acgccctcac gatctcgccg aggaccgtgg agacgcacgt cagcaacatc ctgcacaagc
     2641 tcggcgtccg aaatcgggct gaggccgcgc agcggtaccg acagtcgacc tgaggccgga
     2701 atgggcagga ctctgtcaag acccttgaca agccccctga tgatggcggt cgtggacgac
     2761 gggcgccggt agcgccccag ccgagagccg cgcggctcat gagaaaacca gcctcgtcgt
     2821 ccaccgctat tcccggggag gaatcaaaac gccgggcacg aggcccggcg ttcgtgtttg
     2881 tgcgagccaa gccgcgtcag gcggcggcgg tctccatcgc gggcatgtcg accgcgctga
     2941 ggaagttcca ccacgcatcg agcgctcgct gagtggcgtc ctcgatagcc gtggtgtccg
     3001 tatcgtcgtc ggccgcgatc agcgagccga gcatctcgcg ctcagcaccc gagtgctcga
     3061 tgtcgaggac gccgtggacc gcgaagaagc tcgtggtgcg cttgtcctcg acgtcgtagt
     3121 gcctggccag accgtcaatc ttgctcatgg atgtctgcgg cacctggcgc tcgtatgcat
     3181 atagcgccgc gatcgcggcc gggacggtcg cgttcgtgct caggtcggcg tacagatcga
     3241 cgagctcctg cgtggcctcg ttgcgatcag ccgagcgaac gtcggcgcga tcaacgccga
     3301 tgccctcggc aaagcgcagc cacagctcgg cgtgattggc ctcgccgtgc tcctcgtccc
     3361 acaggttgtc gagtagctgc tggcgcgcag cggcgctctc ggtgcgagtg tggatcgctg
     3421 acaggtagcg cggaaacgac gactcgaacg cgtagtactg gcgcgcgtac tcctgcagcg
     3481 cctccctggg cagcgtgccc tcggaccatt tcgtgtagaa ggggtgcttg agcaagtggc
     3541 gctcgtcgat catcgcgtcg atgcgatcca ggacgtccat tccgcggctc cttacggcta
     3601 gttctgccgt tgagcatagc ggcggccgat gaaacgcttc gacgtcggga tcatcggcgg
     3661 cgggatcatc ggcgccactg cggccgcatt cctggccgag gcgggcagat cggtgttgct
     3721 cttggagcgg ggagggatcg gagcgggcgc ctcgggccgg aactcgggcg taatccagca
     3781 tcccttcgac ccactcttcg caacgctctt ctacgggtca ctcgacctgt accgggaact
     3841 ggcgtcccag gacgatgact tcgccattcc cgacagcccg gctgggctgc tgctcctgtc
     3901 caaagacggg agcgcggttg tggaagcgac gcgaacgatt gcagagagtg cgcccgatct
     3961 gtcgcccgag gtggtcgatc cttccgctct cgcaacgctg gagccggcgc tcgccgaagg
     4021 attgtgggca tgccgactgc acaccggcta cccggtcgaa cctgcggggg cgactcatgc
     4081 ctgggcgcgt cgcgcccgac gcgcgggagc gacgttcgcc ctgggcaagg aagcaaccgt
     4141 ggatatgcgc aacttcgacc aggttctcat cgcggcgggg ccgtggacgc ctgcccttgt
     4201 acccggctgg agcgacgctg caccgatcgt gccgatctgg ggcgtcgtcg tgtccacgac
     4261 gctccgcgac gcgccgcgcc acgtcttgga ggagatgggc atcgctaggt ccggtgaaac
     4321 gcaggaacgc gagttcagcc tggtaacggc gggcggcagg tcgagccttg gatcgacttt
     4381 tctcccccat gagcccgacg cacccgccct ggctccggag ctcattgcgc gcggcgcgca
     4441 gttcgtgccc gagatcggcc gggtgtcaat cgatggtgtt cgcgcgtgtg cgcgacctgt
     4501 gtcgcgcgac ggtcgtcccc tgatcggctt ggtcgacggt cgcgacaacc tgttcgtgtg
     4561 tgccgggcac ggtccgtggg gcatttcgac tgggcccgcg tcggccctga tggtggtgcg
     4621 gcgaatgctc ggccaggctg aggtgcaccc cgctttcgcg gcgcggcgtt tcagcggcct
     4681 tacaactacg gaatttgcat gatgcggccg cgacccgggt cgcttaggat gaacacggtt
     4741 ggccggcgtc ggagggggcg ctggccactt tctctgtccg gcctaggaac gcggcggcag
     4801 gtcacgccac ttaggacccg actcggtcga tgagctcgac cacgaccctg gccggttctg
     4861 cggcgcgggc tgtcgatact gcggcgcggg acgcttcagc acgggctgct gcgacctctg
     4921 ctggggctgc tgctgcattg gtgactcctg cgtgtagatc ggctgccgcg cctgtggctg
     4981 agccggtgca tgttgctgct gcgggggcgc gaaacgcgca ggggccggtt gcgtgtaagg
     5041 ccagtgctgc gcctgtgggg ctgatggtgc gtgagactgt cgttcggcca ctcggtggtc
     5101 cggctgggta tagctatggg acggtggcgc gtactgcacc atgggcgcgg acgatggcat
     5161 gaacgggcga gtcggctcca cggccgagcg ctgcgtgccc ggatcagagt tttcaccggt
     5221 aaagcgtttc cgcagagggc tggcaaatac gatgccggcg acgaccgagc cgacgaggag
     5281 cagcaggatc gtggcgaaga acagcggatt gacctcggca ctcaccgttg tctcggcggc
     5341 aagcctggtc gccggcgcga cagcgactgc cggaatcgag aagccgaaga tcaacgccgg
     5401 agcctgtggg tgaggagtgt ggcagctgca gctccggccg cgagatccgt agcggtgatt
     5461 gcagtccatc gacgtgcagt gtggacacta gcctcgcttg tgtcagccaa cctttggctt
     5521 ctccgttggc accaaacgtc acccttcaca ccggaggtca caggaccgcc gaggaccgcc
     5581 atgacgccac ctaacatcat cacgtgacgc tcggcacgcc cgcacccgct gtcgccgaca
     5641 ggttggccga gccgccctac cgggtacgcg ccaggttgct gaccccgctg ctcaacgggg
     5701 gcgtgacgtt ccacgacgac gctttgctga ccgtcgatga gcgtgggctc atcgcggccg
     5761 tggaggacgc gcgcgttggc ggagacgccg gcgtggacgc gttcgacctc cgtccgatgg
     5821 tgcttatgcc gggcctcatc gacctgcatg cgcacctgcc ccaatacccg aacgctgggc
     5881 tcggcggcgg cctcgacctg ctgacttggc tcgagcgc
//
LOCUS       DATLCO010000146         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9130347, whole genome shotgun sequence.
ACCESSION   DATLCO010000146 DATLCO010000000
VERSION     DATLCO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9130347"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VMZ33_03665"
     CDS             complement(<1..1565)
                     /locus_tag="VMZ33_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUR16360.1"
                     /translation="MDTVYPKRGASRHRLSFACALLAMAVAIPLAPSMGSTSASAMPL
                     ARGSGAVSNGLIVHLKSGVADSSVSTLFAEVGAREVGRFDSRLRIVSPIAALRSTVFA
                     ALTRDPRVERVEYDAEAVKTESPNDTYWDHQWNMRKVRAQDAWSVSKGSPSTIIAVVD
                     TGVDGSHPDLRGRMVGGWDFHNDDRNPDDDDGHGTAVATTAAAAGNDGVGIAGVCWRC
                     RIMPVKVLNSQGHGTHSNIAAGIVWAADHGADVINLSIAGLSSTYMLQNAIAHALRKG
                     VVVVAAAGNDGTSRRIYPAAYPGVISVAATNGIDQLYGWSNRGSWVTMTAPGCSYSGR
                     PHARWGWLCGTSLAAPMVAGAAGLLESLRPGLSRTRLVAALTGTSVGVRASINHGRLD
                     TWRAVRSISGASPDPTPRATPRATPRPTPRPTPRPTPRPTPRPTPLPTPSVEVELWRG
                     SLGRDDRWDRDQYRLQGNVEVALRWSGTDKVSLWITAPNGSVKEHEDDDGQIRLELRL
                     MAGTYTFTVEQHDV"
     gene            1635..2750
                     /locus_tag="VMZ33_03670"
     CDS             1635..2750
                     /locus_tag="VMZ33_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUR16361.1"
                     /translation="MMFKDSAGKQKFTGFLLAADDRPGWATIGHVETAAADGPNRHDP
                     GPEMAGKRALDVLDAGAEPITLPGAAAIRVGTASWTDPTMTAGAVFYPKGSDSAEERL
                     QYYASQFPLVEVDSTYYALPFPKMAELWRDRTPPDFIFDVKAHALMTGQPTETKRLPK
                     TIREELPLELADKSRIYARDLPAELIDVVWQTFLDGLAPLRESGQLGSILMQYPKWFF
                     PISQSREEILAAKERLGGQLFAVELRNASWFNEKNVDRTLRFLTDNEIPFVMVDEPQG
                     FKSSVPPVVAVTSPQLALMRFHGRNTATWEAKGITPAERFRYLYDRDELAEWAPRLRD
                     AARSTRDVHVLMNNCYANYGSTNARELAKILGDLRSD"
     gene            complement(2810..>2995)
                     /locus_tag="VMZ33_03675"
     CDS             complement(2810..>2995)
                     /locus_tag="VMZ33_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16362.1"
                     /translation="VNPVVATFLGALYGESIDVGMIIGAVLIVASVALVIVTESRPTQ
                     PTGALEVQPASPDRSSS"
BASE COUNT          511 a          985 c          988 g          511 t
ORIGIN      
        1 acgtcatgct gctcgacggt gaacgtatac gtgccggcca tgaggcgcag ctcgaggcga
       61 atctgaccgt cgtcgtcttc gtgctccttc acgctgccgt ttggcgcggt gatccacaag
      121 gagaccttgt ccgtccctga ccagcggagg gcgacctcga catttccctg aagtcggtac
      181 tggtcgcggt cccaacggtc gtcgcggcct agacttccgc gccagagctc gacctcaact
      241 gaaggtgtcg gcagaggcgt tggccttgga gtcggccttg gcgtcgggcg aggcgttggc
      301 cttggcgtcg gccgaggcgt ggcccgcggc gtcgcgcgag gtgtcggatc gggcgaggcg
      361 ccagagatgg agcgcaccgc tcgccacgtg tccaggcgac cgtgattgat cgacgcccgt
      421 actcctacgg acgtgcccgt cagcgccgcg accagccgtg tccgggacaa gccgggcctg
      481 aggctttcca ggagccccgc cgcgccggcg accatcggcg cggcaagcga cgtgccgcat
      541 agccatcccc accgagcgtg aggccggccg ctgtacgagc agccgggagc cgtcatcgtc
      601 acccagctgc cgcggttcga ccagccgtac agctgatcaa tgccgttcgt cgcggcgacg
      661 ctgattacgc ccgggtatgc cgcggggtag atccgccgcg aagtgccgtc gttgccagcc
      721 gccgccacga ccaccacgcc cttgcgcaga gcgtgggcta tggcgttctg gagcatgtag
      781 gtggagctga gtccggcgat gctgaggttg atcacgtcgg cgccgtgatc agcggcccag
      841 acgatgcccg cggcgatgtt cgagtgcgtg ccgtggccct ggctgttcag cactttcacg
      901 ggcatgattc gacaccgcca gcacacaccg gcgatgccga caccgtcgtt tcccgctgcc
      961 gccgctgtcg ttgcgaccgc cgtcccatgg ccgtcgtcgt cgtccggatt ccggtcgtcg
     1021 ttgtgaaagt cccagccgcc gaccatgcgg ccgcgcaggt cagggtggct accgtcgacg
     1081 ccggtatcga cgacggcgat gatcgtcgaa gggctgccct ttgagacact ccaggcgtcc
     1141 tgggcacgga ccttgcgcat gttccactgg tggtcccagt aggtgtcgtt gggcgactcg
     1201 gtcttgacgg cttcagcgtc gtactcgact cgctccacac gtgggtcgcg cgtcagggca
     1261 gcaaagactg tggatctcag tgccgcaatg ggagagacga tgcgcagccg tgagtcgaag
     1321 cgcccgactt ccctcgcccc gacctcggca aacaaggtgc tgaccgatga gtcggccaca
     1381 cccgacttca gatggacaat taaaccgttc gagacggcgc cgctccctcg ggcaagcggc
     1441 atcgcgctgg cgctcgtgct gcccatcgat ggcgcaagcg gtatcgccac ggccatagcc
     1501 aggagggcgc aggcgaagct gagacggtgc cttgacgcac cgcgcttcgg gtagacggta
     1561 tccatgactc tcgaacgatt tcctctcgtg gcgatgcggc aacgacccct ctgggcagcg
     1621 gcgtgggtcg tccgatgatg ttcaaagaca gcgcgggcaa gcagaagttc acgggtttct
     1681 tattggcggc tgacgatcgt cccggctggg cgacaatcgg gcacgtggaa accgccgccg
     1741 cagacggccc gaatcggcac gatccagggc ctgaaatggc cggcaaacgg gcactcgacg
     1801 tgctcgatgc cggggctgag ccgatcacgt tgcccggcgc cgcggcgatc cgcgttggca
     1861 cagcctcctg gacggatccg accatgaccg cgggggcggt gttctatccg aaggggtccg
     1921 attcggccga ggaacggctg cagtactacg ccagccagtt ccccttggtg gaggtcgact
     1981 cgacctacta cgccctgccc ttccccaaga tggccgagct ctggcgcgat cgaacgccgc
     2041 ccgacttcat tttcgacgtc aaggcacacg cgctgatgac cggccagccg acggagacca
     2101 agcggctgcc gaaaaccatc agggaggagc tgccgctgga gctcgccgac aagtcacgga
     2161 tctatgcccg cgatctccct gcggagctga tcgacgtggt gtggcagacg tttctcgacg
     2221 gcttggcgcc gctacgcgag tcgggacagc tgggatcgat cctgatgcag tacccgaagt
     2281 ggttcttccc catctcgcag agccgcgaag agatcctcgc ggccaaggag cgcctggggg
     2341 gccagctttt cgctgttgag ttgcgcaacg cttcctggtt caacgagaag aacgtggata
     2401 ggacgctgcg tttcctgacc gataacgaga tccccttcgt catggtcgac gagccgcagg
     2461 gcttcaagtc gagcgttcca cccgtggtgg cagtcacctc accgcagctg gcgctgatgc
     2521 gcttccacgg ccgtaacacg gctacctggg aggccaaggg cataactccg gccgagcgct
     2581 ttcgctacct ctacgatcgg gacgagctgg ccgaatgggc gccacgcctc cgtgacgctg
     2641 ccagaagcac gcgcgatgtg catgtgctca tgaacaactg ctacgccaac tacgggtcga
     2701 cgaacgcgcg cgagctggcc aagatcctgg gcgacctgcg ctccgactaa ccccggcagc
     2761 tggcggtgga cccgcacggc cgcgaattgc gcccgaagga gtccccccac tagctgctgg
     2821 accggtccgg agatgctggc tggacttcga gggccccggt gggttgagtc ggtcgtgact
     2881 cggtcacgat gaccagcgcg acggacgcaa cgatcagcac tgcgccgatg atcatgccga
     2941 cgtcgatcga ctccccatac aacgcgccga ggaacgtggc cacaaccggg ttgac
//
LOCUS       DATLCO010000147         4449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1685769, whole genome shotgun sequence.
ACCESSION   DATLCO010000147 DATLCO010000000
VERSION     DATLCO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685769"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1654)
                     /locus_tag="VMZ33_03680"
     CDS             complement(35..1654)
                     /locus_tag="VMZ33_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUR16363.1"
                     /translation="MLPWTAADLRYRSKLAAWRLRRLGLQPGDRLLTWSPSTPAVPAI
                     YYGAARAGIVVVPLDLRMAPDVIARIAERSDATWLAVGTGLDAPDPGAAGLGDLQIRT
                     VEWLTADPAHQSASQLDEGGLDDPFPQDWEAQVDAWPRPTRDTLFEVIYTSGTTGHPK
                     GVMLKHGTILSTLEAITRLFPAREHRTVSMLPASHLFEQAPVMFFGMMIGADILYLRS
                     RTPRVIFEALREQGVTVMVGVPQLFQLFWNGLEREVKRQGKEKTFRRALNIARYMPYA
                     VRRLIFSRIHTQLGGSLRLMVSAGAYLPSQLQENWERLGIPVIQGYGATECGPAAATS
                     EQDHPTGTVGRTLPPVQVKLAEGSNEILVRGATVFAGYWKDPGATKAVMDGDWYRTGD
                     VGQFDERGNLVLSGRTKNIIVLPNGLNVFPEDIENALADVGLEQAVVIETAPGRIEAV
                     VLSPDAPPVPTASSPAIRPPETAEEMAALRKRIEPMIKQANEKLSQHQRIDDFRVWPD
                     ADFPRTHTLKIRRGEVQRWAGSSVELKTVDD"
     gene            1889..3766
                     /locus_tag="VMZ33_03685"
     CDS             1889..3766
                     /locus_tag="VMZ33_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16364.1"
                     /translation="MIARRYLGAWFLVALVAATCISPVAASPGGRADRVQPAPLNKVD
                     LSERLRADGTYVGRAGVAGSVDLDSWSLTSDLETGEAPRFERLPSAGAGAITPNAWSA
                     LGGGGASPFFGALNAHVYAIEVDGTDVYVGGNFVNAAALPAADFIAKWNGTAWSALGT
                     NQAGTDGALSGPVYALEYSNSYLYVGGQFNNAGGDAEADALAKWHTTGASWSDVAGAT
                     SGPAAIPGQIYALSMYAGSLYVGGYFNNSPATGFTAESDMILMFDGTWHTLGANTVAN
                     PDNGALNGTVRALIATPTGVIAGGDSTDIAGDPSIDRLAEYNTSNNSWAAFNGSGVGF
                     NGTVRALAQSGSTIYVGGDFTDGVGSSNADYVVMWNGSGWAALGTSPLNARVYALAVS
                     GTDVWVAGHFTDAGGMLPADRIVRRNGNTWAALGSNGANDGALNDTVFDPPGAVAAVA
                     VSSNALYAGGSFRNAENIAEADYVAAFGIGSVTNQKPDGRIKKGSGALAGNNVYNTTG
                     VGQTRTGQAAVNGTITFTISIQNDGPQTGKFKVAAIASGNVNYQVTYFRGTTNITSAV
                     VGGTYVTGNVAPGTAWAITAKVKVLPAAIAGSTTTRLVTITSNADSSKVDAVKLIGKR
                     T"
     gene            3862..>4449
                     /locus_tag="VMZ33_03690"
     CDS             3862..>4449
                     /locus_tag="VMZ33_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16365.1"
                     /translation="MTRTRVSLLASGAALTLAISIVSTASAVTRNGWSNVGSNGQPSA
                     PDGALNARVHAIAVSGTDVYVGGMFTGTAGLNGAGSIAKWDGSSWSTMPNSLGGGALN
                     GRVNAILVDGSSVFVGGYFQNAGGDPQADYLAVWNGSTWSDIDGATTGDGALAGEVHS
                     LAKHDSNLYVGGTFFNAPVFGDVSGNAGDNIVRWDG"
BASE COUNT          760 a         1427 c         1471 g          791 t
ORIGIN      
        1 ccgctgggcg gggccgcatt ttcgtttgcc tggcctagtc gtcgaccgtc ttgagctcga
       61 cgctcgagcc ggcccaccgt tgcacctcac cgcgcctgat cttcagggtg tgggtgcggg
      121 gaaagtcggc gtcgggccag acccggaaat cgtcgatccg ctggtgctgg ctgagcttct
      181 cgttggcctg cttgatcatc ggctcgatcc gtttgcgcag cgcggccatc tcctcagcgg
      241 tctcaggcgg cctgatggcg gggctgcttg ccgtcggcac gggtggcgca tccggcgaca
      301 aaacgacggc ctcgatccgg ccaggcgcgg tctcgatcac taccgcctgc tccaacccga
      361 cgtccgcgag cgcgttctcg atgtcctcag ggaacacgtt taggccgttt ggcaggacga
      421 tgatgttctt ggttcgccct gagaggacga ggttgccgcg ctcgtcgaat tgacccacgt
      481 cgccggtccg ataccaatcg ccgtccatca ccgctttcgt cgcaccgggg tccttccagt
      541 agccggcgaa gacagttgcc ccgcggacga ggatctcgtt gctgccctcg gcgagcttga
      601 cttgtaccgg tgggagagtg cgcccaaccg tcccggtcgg gtggtcctgc tcgctcgtcg
      661 ccgcagctgg gccgcattcg gtcgcgccgt agccctggat gaccgggatc ccgagccgct
      721 cccagttctc ctggagctgt gacggtagat aggcgccggc cgacaccatc agccggaggc
      781 ttccgccgag ctgggtgtgg atccgactaa atatcagtcg acggaccgcg taaggcatgt
      841 agcgcgcgat gttgagggcc cgtcggaagg tcttttcctt gccctgccgc ttgacctcgc
      901 gctcgaggcc gttccagaag agctggaaca gctgcgggac gccaaccatc acggtgacac
      961 cttgctcgcg caaggcttcg aagatcactc ttggtgttcg cgagcgcagg tagaggatgt
     1021 cggcgccgat catcattcca aaaaacatca cgggcgcctg ctcgaataga tgcgaggcgg
     1081 gcagcatgct gacggtccga tgctcacgcg ctgggaacag gcgtgtgatc gcctcaagcg
     1141 tcgacaggat cgtcccgtgc ttgagcatca cgcccttggg atggcccgtc gtgcctgacg
     1201 tgtagatcac ttcgaagagc gtgtcccgag ttgggcgcgg ccaggcgtca acctgtgcct
     1261 cccaatcctg cggaaacggg tcgtcgaggc cgccctcgtc gagctggctg gcactctggt
     1321 gggcagggtc ggcggtgagc cactccacgg tgcgtatctg caggtcgccc aggccggcag
     1381 cgccggggtc gggtgcatcc aggcctgtgc ccacggcgag ccacgtggca tcggacctct
     1441 cggcgatgcg ggcaatgacg tcgggggcca tgcgcaggtc gagcgggacg acgacaatcc
     1501 cggcgcgcgc ggcgccgtag tagatggccg gcacagctgg cgtcgacggg ctccaggtca
     1561 ggaggcggtc ccccggctgg aggcccagcc gtcgcagacg ccacgcagca agctttgatc
     1621 ggtaccgaag gtccgccgct gtccagggaa gcacgagctc gtcgtcggtt cgcagcgcga
     1681 actggcgccg cccagcccag cgggtcgcag aatcgtcgag aaggtcgagc agcgactcaa
     1741 actgccgcgg atcaggtatt tcccacgtgg atgcgctgat tggggcctcc ctcgatcggt
     1801 cgggcggagt ctattgcttt cgccttcgac gtcggatagc gtcagctgac tgcgcgttgc
     1861 cgcgccctag agcctgaggg ggttcatcgt gatcgctcgc cgttaccttg gcgcatggtt
     1921 cctcgtggct cttgtcgccg cgacctgcat cagccctgtg gcggcgtcgc ccggtggccg
     1981 ggctgatcgt gtccagccag ctccgctgaa caaggtcgat ctgagcgaac gcttgagggc
     2041 cgatgggacc tatgtcggcc gagctggcgt ggctggttct gtcgacctgg attcatggtc
     2101 cttgaccagc gatctcgaaa caggggaggc cccacgcttc gagcggctcc ccagcgccgg
     2161 tgctggcgcg atcacaccca acgcctggtc ggccctgggt ggcggcggcg ccagtccgtt
     2221 cttcggcgcg ctgaatgccc atgtctatgc gatcgaagtc gacggaacgg atgtctacgt
     2281 gggcggaaac ttcgtcaacg cagcagcgct cccggccgcc gatttcatcg ccaagtggaa
     2341 cgggacggca tggtcggccc tgggcactaa ccaggctggc acggacggag cactcagcgg
     2401 gcctgtttat gcgctggagt actccaacag ctatctgtac gtcggcgggc agttcaacaa
     2461 tgccggcggc gacgccgaag cggacgcatt ggcaaagtgg cacacgacgg gcgcatcctg
     2521 gtcggatgtc gccggcgcaa cctcggggcc agcggcgatt cctggccaga tctatgcgct
     2581 gtcgatgtac gcgggcagcc tgtatgtagg cgggtacttc aacaattcgc cagcgacggg
     2641 ctttaccgcg gaaagcgaca tgatcctgat gtttgacggt acgtggcata ccctcggcgc
     2701 gaataccgtc gcgaatccag ataacggcgc cttgaatggc acggtgcgag ccctgattgc
     2761 cactccgacg ggcgtgattg ccggtggcga ctcaaccgac atcgcaggtg atccgtcgat
     2821 cgaccggctc gccgaataca acaccagcaa caacagctgg gcagcgttca atggcagtgg
     2881 ggtgggcttc aacgggacgg tccgcgcact tgctcagtcc ggcagcacca tttatgtcgg
     2941 tggcgatttc acggatgggg tggggtccag caacgctgac tacgtcgtca tgtggaacgg
     3001 ctccggctgg gctgcgctcg gcacgagccc gctgaacgca cgcgtctacg ctcttgcggt
     3061 ttcgggaact gacgtgtggg ttgcgggcca cttcaccgac gccggcggca tgctgccggc
     3121 cgatcgtatc gtgcgcagga atggcaacac ctgggcggcc ctcggctcca acggagcgaa
     3181 cgacggggcg ctgaacgaca cggtgtttga cccccctggc gcggtcgctg ccgtcgcggt
     3241 gtcgtcgaac gcgctgtatg cgggcggcag cttcaggaat gccgaaaaca ttgccgaggc
     3301 cgactacgtc gccgcctttg gcatcgggtc ggtcacgaac caaaagccgg acgggcgcat
     3361 caagaagggg agtggcgccc ttgccggcaa caacgtctac aacacgaccg gcgtgggcca
     3421 gacccgcacc ggacaggccg cggttaacgg cactatcacc ttcacgattt ccatccagaa
     3481 cgacgggccg cagacgggca agttcaaggt cgccgcgata gcgtcgggca acgtcaatta
     3541 ccaggtcacc tatttccgag gcaccacgaa catcacctct gctgtcgtgg gtggaaccta
     3601 cgtgaccggc aacgtcgcac ctggcaccgc ctgggcgata actgccaagg tcaaggtgct
     3661 tccagcggcg atagccggct cgaccaccac gaggttggtc acgatcacct ccaacgccga
     3721 ctcgtccaag gtcgacgccg tcaagctcat cggcaagcgg acctagtcgt gggcgcgcaa
     3781 gcgcctgcaa ccaaactgca accggctccg aggatgcttc gcgctagccg tgaagaccgc
     3841 ccggaggtga aacctgcagc catgacacgt actcgcgtct cgctcctcgc aagcggcgcc
     3901 gccctgacgc tcgctatttc gatagtctcc accgccagcg cggtgacacg caatggctgg
     3961 tcgaacgttg gttcgaacgg ccagcccagc gcgcccgacg gcgcactcaa cgctcgcgtg
     4021 catgcgatcg ccgtctcggg gaccgacgtc tacgtcggcg ggatgttcac cggcaccgct
     4081 gggctcaacg gcgcaggctc tatcgccaaa tgggacgggt catcgtggtc gacgatgcct
     4141 aactcccttg gtggcggcgc gctcaatggc cgagtgaacg cgatcctggt cgacggatcg
     4201 agcgtgttcg tcggcggcta ctttcaaaac gcagggggag acccgcaggc cgactatctg
     4261 gcggtctgga acggatcgac ctggtccgac atcgacggag caacgacggg cgacggcgcg
     4321 ctcgccggcg aagtgcactc gctggccaag cacgattcaa acttgtacgt cggcggaact
     4381 ttcttcaacg cgccagtctt tggcgacgtt tccggcaacg cgggcgacaa catcgtccgc
     4441 tgggacggc
//
LOCUS       DATLCO010000148         3776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9136736, whole genome shotgun sequence.
ACCESSION   DATLCO010000148 DATLCO010000000
VERSION     DATLCO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9136736"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1992
                     /locus_tag="VMZ33_03695"
     CDS             <1..1992
                     /locus_tag="VMZ33_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HUR16366.1"
                     /translation="RLAADPRVTRVEGLVDVDPRLRRDQYQLLYSAPGGPADRFVAQT
                     LAATTRDDLTTFTIYTAYGPNRAEAQALVVDMRDKGSALAPPPGMTVLVGGGAAEVRD
                     VVARIGSDFPLSAAFILISTYLVLFVLLRSVVLPAKALIVNGLSLTASFGALVWIFQD
                     GNLSAILGFQPLGFVETTLPVILFCVLFGLSMDYEVFLLSRMKEVYDRTGDNEEAVAA
                     GMERSGRIVSSAALIVVVVAGSFAFADIVLIKALGLGVAIAVALDATLVRALLVPSTM
                     RLLGDVNWWMPERLKRVIPPLAVALLLVGCSASGAILANEPAPQPSISAPTAAPSRQP
                     DPQPVVLPRDEAAHERLTEWWYYTGHLEADDGSEFGFEFVIFRAERGSFPVSWASHLA
                     LTDERGHRFLYEQRSEIGPQADLTAEAGFNLSVRGTSDIGLPLPGEPWRMSGVGGRDV
                     LTATGSAFAFGLELVDTRSDVVLHDSEGWIDFGPAGGSYYYSRPRMAATGDLTIDGRA
                     MSVEGSAWFDHQWGDFIAVGGGGWDWFAVNLEDGTDLTISLVRAADGSYPLIYGTLVN
                     ADGTYEHLPRGAFELTPRGTWTSERTGAVYPSGWTITIPTEELVVDLTPTIEDQELDT
                     RPTTGVVYWEGSQFVAATRDGEAVGGEAYVELTGYSASQ"
     gene            complement(1986..3611)
                     /locus_tag="VMZ33_03700"
     CDS             complement(1986..3611)
                     /locus_tag="VMZ33_03700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HUR16367.1"
                     /translation="MIAGAVLALFATVALFGLLNKPVNTTGASLPSGSTEVTPAPSGI
                     ATTGVGETPTPTATGSTGTEPTATPTATATATPPPTPTPPPDAVAIPFVPVVGFWSTD
                     ASITVANLRAAAGGTNTKYPELLLPIDDEAAVAAALGVTGYQTGTVDQIEAAVKAGAV
                     GILRATDVTPRVHALPLNGVALFGNDRVRDLAQWPLTLQVSSSETWNQAGAWTLVAGG
                     DIMMDRGNRRAIERHGNDPDYVLDGGTSRVTGVRCCSIFGYEYVTTRRTGNTGAMRDL
                     FSTADVAMANLESAVLVNAPYHATGFIFTTPAAYLDALETAGIDFLSLGNNHIRNAAA
                     RGITTAITELDARGIEHSGAGPFPDNSKPGVVEVNGLRVAFLGCDAIRPGWAATPDKV
                     GTFNCRSGDVAGKIRQMRPNYDVIVVMPHWGREYAAKPADYQRVLAGAWLAAGADVVI
                     GAHSHWAGGMQDFDGKLALYSIGNFVFDQSWSRSTMQGVVPELTFVGTELVQVNLHPT
                     LLIDSQPNFVAPTQGGQYVIDQMIRGSQGLLNY"
     gene            complement(3669..3745)
                     /locus_tag="VMZ33_03705"
     tRNA            complement(3669..3745)
                     /locus_tag="VMZ33_03705"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3709..3711),aa:Asp,seq:gtc)
BASE COUNT          581 a         1229 c         1296 g          670 t
ORIGIN      
        1 cgcctggcgg cggatccgcg agtgacgcgc gttgaagggc tcgtcgatgt cgacccgcgg
       61 ctgcgccggg accagtacca gctgctctac tcggcgcccg gtggtccggc cgatcgtttc
      121 gtcgcgcaga cgttggcggc cacgaccagg gacgacctga caaccttcac gatctacacg
      181 gcgtacggac cgaaccgggc cgaggcccag gccctggtcg tcgacatgcg cgacaagggc
      241 tcagccctcg cgccgccgcc cgggatgacc gtgctggtcg gcggcggcgc agcggaggtc
      301 cgcgacgtgg tcgcgcgcat cggctcggac ttcccccttt cggcggcgtt catcctgatc
      361 tcgacgtacc tggtgctgtt cgtccttctt cgttcggtgg tcctgccagc gaaggccttg
      421 atcgtcaacg gcctgtcgct gaccgcgagc tttggcgcac tcgtgtggat cttccaggac
      481 ggcaacctgt cggcgatcct cggcttccag ccgctcgggt tcgtcgagac gacactcccc
      541 gtgatcctgt tctgcgtcct cttcgggctg tccatggact acgaggtgtt cctgctgtcg
      601 cgcatgaagg aggtctacga ccggaccggt gacaacgagg aagccgtagc cgccggcatg
      661 gaacgcagcg gccgcatcgt gagctccgca gcgctcatcg ttgtcgtcgt cgccggctcg
      721 ttcgcttttg ccgacatcgt cttgatcaag gcgctcggtc tcggggtggc gattgccgtg
      781 gctcttgacg cgacactcgt gcgcgctctt ctcgtgccat cgacgatgcg cctgctcggg
      841 gacgtgaact ggtggatgcc cgagcggctg aaacgtgtca tcccgcccct cgcggtcgcg
      901 ctgctcctcg ttggctgctc ggcgtccggg gcgatcctgg ccaacgaacc ggcacctcag
      961 ccctcgatct cggcaccgac agccgcgcca tctcgtcagc ccgatccgca gccggtcgtc
     1021 ctgccccggg atgaggccgc gcacgaacga ctgaccgaat ggtggtacta caccggccat
     1081 ctcgaggccg atgacggcag cgagttcggc ttcgagttcg tcatcttccg cgccgagcgg
     1141 ggttccttcc cggtgtcgtg ggcatcccac ctggcgctca cggatgagcg cggccacagg
     1201 ttcctgtacg agcagcgatc ggagatcggg ccccaagccg atctgacggc agaagcaggc
     1261 ttcaatctgt cggtgcgggg aacgtcggat atcgggttgc cacttccggg cgagccgtgg
     1321 cgcatgtccg gcgtcggtgg ccgggatgtt ctgaccgcaa cgggcagcgc cttcgcgttc
     1381 ggcctggagc tcgtggacac ccgatcggac gtcgtgcttc acgacagcga agggtggatc
     1441 gacttcggcc cggctggcgg ctcgtactac tactcacggc cacggatggc agcaaccggc
     1501 gacctgacga ttgacggcag ggccatgtcg gtcgagggca gcgcctggtt cgaccatcag
     1561 tggggggact tcattgccgt cggcgggggt ggctgggact ggttcgccgt gaacctcgag
     1621 gacggcacgg acctcacgat ctcgctcgtc agggcggccg acggcagcta tcccttgata
     1681 tatgggacgc tcgtcaacgc ggacggcacg tacgagcacc tgccccgcgg ggcgtttgag
     1741 ctgacaccca ggggtacgtg gacaagcgag aggactggcg cggtctaccc ctcaggctgg
     1801 actatcacca tccccacgga ggagctcgtc gttgacctga cgcccaccat cgaggaccag
     1861 gagctcgaca cacgcccgac aaccggcgtc gtgtactggg agggatcgca gttcgtcgcg
     1921 gccacgcgcg atggcgaagc ggttggtggc gaggcctacg tcgagctgac gggatattca
     1981 gcttctcagt agttgaggag cccctgcgag ccgcggatca tctggtcgat tacgtactga
     2041 ccgccctggg tcggcgcaac gaagttcggc tgcgagtcga tgagcaacgt tgggtggagg
     2101 ttgacctgca ccagctccgt tccgacgaac gtcagctccg gaacgacgcc ctgcatcgtc
     2161 gatcgcgacc agctctggtc gaagacgaag ttgccgatcg agtagagggc cagcttcccg
     2221 tcgaagtcct gcattccgcc cgcccagtgc gaatgcgcgc cgatgacaac atcggcaccc
     2281 gctgcgagcc aggcgccggc caggacacgc tgataatcgg ccggcttggc ggcgtactcg
     2341 cggccccagt ggggcatgac gacgatgacg tcgtagttgg gccgcatctg gcggatcttc
     2401 ccggccacgt cgccgctgcg gcagttgaac gtgccgacct tgtcgggcgt ggccgcccag
     2461 cccgggcgga tggcgtcgca gcccaggaac gcgacgcgga ggccgttgac ctcaacgaca
     2521 ccaggcttgc tgttgtccgg gaacgggccg gcgccgctgt gctcgatgcc gcgcgcgtcg
     2581 agctcggtga tcgcggtggt gatgccccgc gccgccgcgt tgcggatgtg gttgttgccg
     2641 agcgacagga agtcgatgcc ggcggtctcc agtgcatcga ggtacgcggc tggcgtcgtg
     2701 aagatgaacc cggtggcgtg atagggcgcg ttgaccagca ccgccgattc aagattggcc
     2761 atggccacgt ctgcggttga gaagaggtcg cgcattgccc cggtgttccc ggtgcgtcgc
     2821 gtcgtcacgt actcgtagcc gaaaatggaa cagcagcgaa cgccggtcac ccggctggtg
     2881 ccaccgtcca ggacatagtc agggtcattg ccgtggcgct cgattgccct gcggttaccg
     2941 cggtccatca tgatgtcgcc gcccgccacc aacgtccagg cgccggcttg attccaggtc
     3001 tcgctcgagc tcacctggag tgtcagcggc cattgcgcca ggtcgcgcac gcgatcgttg
     3061 ccgaacagcg cgacgccgtt gagcggcagc gcgtggacgc gaggcgtcac gtcggttgcg
     3121 cgaaggatgc ctactgcacc ggccttcact gcagcctcga tctggtcgac cgtgcccgtc
     3181 tggtagccag tgaccccgag tgccgccgca acagctgcct cgtcgtcgat tggtaaaagc
     3241 agctcggggt acttggtgtt ggtgccgccg gcagccgctc tgaggttggc caccgtgatg
     3301 ctcgcgtcag tcgaccagaa accgacgacc ggcacgaagg ggatggcgac cgcatcgggc
     3361 ggaggcgtcg gggtcggtgg aggtgtcgcc gtcgcggtcg cagtcggcgt ggccgtcggc
     3421 tccgtccctg ttgatccggt cgcggtaggt gtcggagtct cgcccacgcc agtcgtcgcg
     3481 atgccgcttg gtgcgggggt tacctcggtg gaaccgctgg ggagcgaagc gccggttgtg
     3541 ttgaccggct tgttgaggag gccgaacaac gcgaccgtcg caaagagcgc cagcaccgcg
     3601 ccggcaatca cgagccctcg gttagcggtc agctcaaatc ccctatggcg cccgcttcag
     3661 gcgagaattg gcggacccga ccggattcga accggcgatc tccagcgtga caggctggca
     3721 tgttgggccg ctacaccacg ggtccgggca gcgagcgagc aggataccag ccctcc
//
LOCUS       DATLCO010000149         4584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4664433, whole genome shotgun sequence.
ACCESSION   DATLCO010000149 DATLCO010000000
VERSION     DATLCO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4664433"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VMZ33_03710"
     CDS             complement(<1..855)
                     /locus_tag="VMZ33_03710"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16368.1"
                     /translation="MATIQLSGVSRSFAIGGQPLTVLDDVNLNVASGSVVAVVGPNGS
                     GKSTLLRLICGLLRPDAGEILINGRAVGEADERVGLVFQEPRLLPWRRVIDNVAFPLE
                     LVGVARDQRNTRARELLDLVGVTGFGEAYPHQLSGGMRQRVAMARALARDPEILLLDE
                     PFSALDALTRERLDAELLQLWQRIGATVVVVTHSIPEAVFLADQVCVLSARPGRLVAT
                     VPVDVARPRTAATFDAPAFIRAAAAVRAHLSGTALEQARDEAAAAPMRDVLDRSGSKA
                     WFDPFGTSG"
     gene            1211..1864
                     /locus_tag="VMZ33_03715"
     CDS             1211..1864
                     /locus_tag="VMZ33_03715"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUR16369.1"
                     /translation="MLTVLLTRHGHTDKSEPEQYLGQRLVAKLTDRGLHDAHALADRL
                     TGVRVDRLISSPLARAVETAEILAPAVGATVETDARLSELDYGAWEGLTVEELDARFP
                     GARELYEIDPAMHNVGGGESGIEVAARVSDLIDSLLSWGLADDTDRTALLVGHSSVNR
                     VLLALVMGVGLRDYRRRFRQDWTSLTVVRWQDRESGPLLMLANDVSHTRGLAGATWE"
     gene            1855..3408
                     /gene="hutH"
                     /locus_tag="VMZ33_03720"
     CDS             1855..3408
                     /gene="hutH"
                     /locus_tag="VMZ33_03720"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HUR16370.1"
                     /translation="MGVISLNGLKLTLEEVEDVARGRADVRLDDAARERMHASWLVVD
                     ELVATDEMVYGVTTGFGALATHRIVHKDAARLQENLLVSHAVGVGPTHGRNTTRAMLL
                     LRANALARGQSGCRPELVDRLLDFLRLGIHPQVPEQGSVGASGDLAPLAHLALPLIGR
                     GQAEVDGEVISGAEALAAKGLTPIALAPKEGLALLNGTQQMTAIGVLVLLHAERLLNT
                     ASVVAAMSTEALLGTDVAFAAAYHEARPHPGQLRVATQLRHLLRDSELMRSHHTDAHK
                     VQDPYSIRCVPQVHGTVADALAYCRGVLEIEVNSATDNPLVFPEGADVDATALATGGG
                     RVISGGNFHGQPVALAMDLLAIAVAELGSISERRIAQLIDGRQSGLPPFLVENAGLNT
                     GLMLHQYTAAALVSENKVLAHPASVDSIPTSANQEDHVSMGPIAARQAREVVRNVEQV
                     LGLELLCSAQGLDFRLATGLRAGAGVGDAHARIRARVAHLDDDRDPQPDLRAAIELVR
                     SGALVDLVA"
     gene            3405..4160
                     /locus_tag="VMZ33_03725"
     CDS             3405..4160
                     /locus_tag="VMZ33_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16371.1"
                     /translation="MTKMAESRIIDISDSSSFGLIPPCVDPRFDHRTCDYWEDADRGS
                     KVNRPAWLEVAGPAPAARSDSLADNPFAPAPRSGGGVNPFSVGDDDVFGVPGDNPFAP
                     VVQRERPLRDGVPRKLALLDRGRGIFGSYGKVLLIDDEAVAYSQFGPLSAYPRATRLR
                     ELYPQLPDAPLPAVITCVAATAEALRQGYATQLADAVCRDLTARGFAAVEAYPDLTRP
                     ETETSAARPAFWERLGFAIAVDDERFPVMRREL"
     gene            4157..>4584
                     /locus_tag="VMZ33_03730"
     CDS             4157..>4584
                     /locus_tag="VMZ33_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HUR16372.1"
                     /translation="MSQPDVVTPATLALDVAGVPYQLVVTQPANSAEESARFQGIALD
                     QLLRTIVVRRAADDYLFVLVPGGRRFDWPKLRTHLGAKRLSLPDAEEAKAVTGYERGA
                     ITPFGSSNAWPVVADAAILAQQLVAIGGGRRGVQIHIAPDA"
BASE COUNT          734 a         1603 c         1546 g          701 t
ORIGIN      
        1 gccgctcgtt ccgaacggat cgaaccacgc cttcgagccc gatcgatcga ggacatcgcg
       61 catcggcgcg gcagcagcct cgtcgcgcgc ctgctcgagg gcagtcccac tcaggtgggc
      121 acggacggcg gcggcggcac ggatgaaggc gggcgcatcg aacgtggccg cggtccgcgg
      181 ccgtgcgacg tcaacgggca cggtcgcgac caagcggccg gggcgagcgg aaagaacaca
      241 gacctggtcg gcgagaaaca cggcctcagg gatgctgtgc gtcacgacca cgaccgtggc
      301 gccgatgcgc tgccagagct gcagcagctc ggcgtcaagt cgctcgcgcg tcagcgcgtc
      361 gagggcgctg aatggctcgt caagcagcag gatctccggg tcgcgcgcga gcgcgcgggc
      421 catggccacg cgctggcgca tgccgcccga cagctggtgg ggatacgcct cgccgaagcc
      481 agtcactccc acgaggtcca gcagctcccg agcccgggtg tttcgttggt cgcgggcgac
      541 gccgacgagc tccagtggaa acgcgacgtt gtcgatgact cgtcgccacg gcaacaggcg
      601 cggctcctgg aatacgaggc cgacgcgctc gtcggcttcg ccgacggcac ggccgttgat
      661 cagaatttcc ccggcgtcgg gccgcagcaa gccacagatg agccgcagga gcgtgctctt
      721 gcctgatccg ttgggcccga ccacggccac aactgagccg ctggcgacgt tgaggttcac
      781 gtcgtcgagc acggtcagcg gctgaccgcc gatcgcgaac gaacgcgaaa cgcccgataa
      841 ctggatggtt gccacgtctg ccgcttgctc tccattgctg cgccaacgtc aatcacggga
      901 caacaggcga gcggaagcgt gcacgcgcac acgatgtgtc ggcgcgcttc gtttctccca
      961 tccggactct taccgtcggc tccctgccag gggatccacc gccgccgcgc gaacgcgacg
     1021 ccgggtcgtg ggctggcacc gggcaccgct cgcgcagccc cgacaccgac caccggtcgg
     1081 gaatttcacc caaccccgaa acgaaggcgt attcgtgttg tgcgccgagc atagccggaa
     1141 ctgatcggga ggcatcccgc cgagaccgcg gcggccgcag ggcagacgct cgccccggta
     1201 tcgtggccac gtgcttaccg ttctcctcac acgccacggc cacaccgata agtcggagcc
     1261 cgagcagtat ctcggccagc gcctcgtcgc caagctcacc gatcgcggct tgcacgacgc
     1321 gcacgccctg gccgaccgac tgaccggtgt ccgggtcgac cgcctgatct cgagccccct
     1381 cgcccgagcc gtcgagacgg cggagattct cgctcctgcg gtgggcgcaa cggttgaaac
     1441 ggacgctcgc ctgagcgagc tcgactacgg cgcgtgggag ggcctgactg tcgaggagct
     1501 cgacgcccga ttcccgggcg cgcgtgagct gtacgaaatc gacccggcca tgcacaacgt
     1561 cggcggcggc gaaagtggca tcgaggtcgc cgcgcgcgtg agcgatctca tcgactcgct
     1621 gctcagctgg ggcctcgccg acgataccga caggaccgct ctactcgtcg ggcactcgtc
     1681 cgtgaatcgg gtgctgctgg cgctggtcat gggcgtggga ctgcgcgact atcggcgccg
     1741 cttccgccag gactggacca gcctcacggt cgtgcgctgg caggaccgtg aatcgggacc
     1801 gctcttgatg ctggccaatg acgtgtcgca cactcgaggc ctggcgggcg cgacgtggga
     1861 gtaatcagtc tcaacggcct aaagctcacc ctcgaagagg tcgaggacgt tgcccgcggg
     1921 cgcgccgacg tgcgcctcga cgatgcagcg cgtgagcgta tgcatgccag ctggctcgtc
     1981 gtcgacgagt tggtcgcgac ggacgagatg gtctatggcg tgacaacggg tttcggggct
     2041 ttggccacac accggatcgt ccacaaggat gccgcgcgac tccaggagaa cctcctggtg
     2101 agccacgcgg tcggcgtggg gccgacgcac ggccgcaaca cgacgcgcgc catgttgttg
     2161 ttgcgtgcca acgcccttgc gcgcggccag agcggctgcc ggccggagct ggtcgatcgg
     2221 ctgctcgact tcctgcgact gggcatccac ccgcaggtcc ccgagcaggg atccgtcggc
     2281 gcgtcagggg acctggcgcc gctggcgcac ctggctctgc cgttgattgg ccgcggacag
     2341 gcggaagttg acggcgaggt aatcagcgga gcggaggcgc tcgcagccaa agggctaaca
     2401 cccattgccc ttgccccgaa agagggtctg gcgctcctca acggcaccca acagatgacc
     2461 gcgatcggcg tcctcgtgct gctgcacgcc gaaaggctgc tcaacacggc atcggtcgtc
     2521 gcggcgatga gcaccgaggc gctgctgggc acggacgtcg cgttcgcagc cgcctatcac
     2581 gaggcgcgtc cgcacccggg ccagctccgc gtcgctacgc agctgcgaca ccttctgcgc
     2641 gactcggagc tcatgaggtc ccatcacaca gatgcgcaca aggtccagga cccgtactcc
     2701 atccgctgtg tcccgcaggt ccacggcacg gtcgccgacg cgctcgcgta ctgccgcggc
     2761 gtcctcgaga tcgaggtcaa ctccgccacc gacaatccgc tcgtcttccc ggagggcgcc
     2821 gacgtcgacg ccacggccct ggccaccggc ggcggccgag tcatctcggg cggcaacttc
     2881 cacggtcagc ccgtggcctt ggcgatggac ctcctggcaa ttgccgtcgc ggagctgggc
     2941 tccatcagcg agcgcagaat cgcccagctg atcgacggcc gccagtcggg cctgccgccg
     3001 ttcctcgtcg agaacgccgg cctcaacacc gggctgatgc tccaccagta caccgcggcg
     3061 gcccttgtgt cggagaacaa ggtgctcgcc cacccggcct cggtcgactc gatcccgacg
     3121 agcgccaatc aggaggatca cgtctcgatg ggcccgatcg ccgcccgtca ggcgcgcgag
     3181 gtggtgcgca atgtcgagca ggtgctgggc ctcgaactgt tgtgctcggc gcaggggctc
     3241 gatttccggc tggctacggg gcttcgtgca ggcgccggcg tcggtgatgc acacgcccga
     3301 atacgcgcac gggtggccca cctcgacgac gatcgggacc cgcagccaga cctgcgagca
     3361 gcaatcgaac tcgttcgcag cggagcgctc gtcgacctcg tggcatgacc aagatggccg
     3421 agtcgcggat catcgatatc tcggatagct caagctttgg cctgattccc ccgtgcgtcg
     3481 atccgcggtt cgaccaccgg acgtgcgact actgggagga cgctgaccgg ggctcgaagg
     3541 tcaatcggcc ggcgtggctg gaggtcgctg gaccggcacc agcagctcgg agtgactcac
     3601 tggccgacaa cccgtttgcc ccagcgccga ggtcgggagg cggcgtcaac ccgttttcgg
     3661 tgggcgacga cgacgtgttc ggtgtcccgg gcgacaaccc attcgcgccg gtcgtgcaac
     3721 gtgagcggcc tctccgcgac ggagtgccgc gcaagctggc gctactcgac cgtgggcgtg
     3781 gcatattcgg ctcgtacggc aaggtcttgc tcattgacga cgaggccgtt gcgtactccc
     3841 aattcggtcc gttgtccgcc tatccacgcg caacgcgcct gcgcgagctc tatccgcagc
     3901 tccccgatgc gccactcccc gccgtcatca cctgcgtcgc cgcgaccgcc gaggcgcttc
     3961 ggcagggtta cgcgacgcag ctcgccgacg ccgtttgccg cgacctcact gcgcgtggct
     4021 ttgccgcggt ggaggcttac cccgacctga cgcgtccgga gacggagacg agcgctgcgc
     4081 gcccggcgtt ctgggagcgc ctcgggttcg ccatcgcggt agacgacgag cgcttcccgg
     4141 tcatgcggcg cgaattgtga gccaaccgga cgtggtcacg ccggcgaccc tggccctcga
     4201 cgtcgccgga gtcccgtacc agctcgtcgt cacgcagccg gcgaacagcg ccgaggagag
     4261 cgcacggttc cagggcatcg ctctggatca gctgctgcgg acgatcgtcg tgcgtcgcgc
     4321 agccgacgac tacctcttcg tgctggttcc aggcggtcgg cgcttcgact ggccaaagct
     4381 ccggacacac cttggcgcta agcgtctctc gctgcccgac gccgaggagg ccaaggctgt
     4441 gaccgggtac gagcgcggcg ccataacgcc cttcggatcg agcaatgcct ggccggtcgt
     4501 cgccgacgcg gccatcctcg cccagcagct ggtcgccatt ggcggtgggc gacgcggcgt
     4561 ccaaatccac atcgcgcccg acgc
//
LOCUS       DATLCO010000150         3175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6808294, whole genome shotgun sequence.
ACCESSION   DATLCO010000150 DATLCO010000000
VERSION     DATLCO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6808294"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="VMZ33_03735"
     CDS             <1..1866
                     /locus_tag="VMZ33_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16373.1"
                     /translation="SGTGSPADAVIGYLRDRQALLLLDNFEQIIEAATEVGRLVREAP
                     GIKVIVTTRIVLRVYGERDFPVPPLGLPPPGGVALPAEEAAEYEAVRLFVERARAVQP
                     AFSLTDDNAQHVVEICRRLDGLPLAIELAAARTRLLSVAAIHARLDSHLALLTGGSRD
                     LPGRQQTLRGAIDWSYELLEQADRKLFERFSVHAGGAFLSQADEVCGPAQELGEDVLD
                     GLSSLGEKSLVKPDLGVDEDPRFTMLTTIRDYAHERLEKEQDFEQLARRHAHAYLGLV
                     EGCSDRLLGRESRRLSDRLEQDHDNLRAALDWAVAHQEVDFALRFMASVWRFWQTRGH
                     LDEARRRAEIVIGLPGVPDQPKDLLARAYAAVGGIAYWQADVRATHRYYTLGLEAARA
                     SGDERLVGQALYNLSFAPLAQDTLTSALYVAGRPHLEDAIAIYEQLGDKGGLADAKWA
                     LSQATGAMGDTEGAMAYAVSALADYRDLNDPFRTGWGLYMVAGLRAVGGDVPGSLSLL
                     RESLSIFADARDRSGILLILAGYAVASSLLGDQEFAARTGGVVETLRASTGAGLIDAT
                     PEFIQFQLPKRPDDPHLVEVWEAGARISLEEAVAETLEWSAAAEEAADNAQVIAE"
     gene            1890..2570
                     /locus_tag="VMZ33_03740"
     CDS             1890..2570
                     /locus_tag="VMZ33_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16374.1"
                     /translation="MKRRMRKHHELGTSYGNGVVGPSRKQVRAAIETLPENLGWTELA
                     ESLRPVFVRRRPLPPGIERPVTTLRPPGLEVALGVDIGPAFMYVGHGMLETWGVTEAD
                     AFERAMANLRSSVAAEKYVEMEYESIDGVPFWWYQSQGGLASGLLLLEDELATRYGPD
                     PRLLIAPMRNLLLAAPFDADRSLVSWLRDEISYDDPNGLDLPIFALIDGRLSIDTRVE
                     RLQAAVVH"
     gene            complement(2564..2953)
                     /locus_tag="VMZ33_03745"
     CDS             complement(2564..2953)
                     /locus_tag="VMZ33_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16375.1"
                     /translation="MRLGRKSRPLLLLWGARASNSYVDLNDELDAHFGMFRFRTPTSN
                     IARWSAEGPWLWITAIGVRRGILNGEMSFVGAHTGGVRLEFKERPKWGLLRPPAFWVA
                     VDDIDGFTASLAARGIPGEDKRKRRTQ"
     gene            complement(3019..3095)
                     /locus_tag="VMZ33_03750"
     tRNA            complement(3019..3095)
                     /locus_tag="VMZ33_03750"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3059..3061),aa:Pro,seq:cgg)
BASE COUNT          543 a         1054 c         1043 g          535 t
ORIGIN      
        1 tccggtactg gctcgccggc tgacgccgtg atcggctatc tccgagatcg acaggcgctg
       61 ctcctgctcg acaacttcga gcagatcatc gaggccgcaa cagaggtcgg gcgactcgtt
      121 cgggaagcgc cgggcatcaa ggtcatcgtt accacccgaa tcgtcctgcg cgtctacggt
      181 gagcgtgact ttccggttcc gccactcggc ctgccgccgc ccggcggtgt cgcgcttcct
      241 gccgaagagg ccgccgagta cgaggcagtc cggctcttcg tggagcgggc acgtgccgtc
      301 cagccggcgt tctcgctgac cgacgacaac gcgcagcacg tcgtcgagat atgccgccgt
      361 ctcgacggcc tgccgctcgc catcgagctc gccgcggccc gtacgcggct cctttccgtg
      421 gcggcaattc acgcccgtct cgactcgcac ctggctctcc tgaccggcgg ctcgcgtgac
      481 ctgcccggcc gccaacagac gctccgcggt gcgatcgact ggagctacga gctcctcgag
      541 caggccgatc gcaaactttt cgagcggttc tctgtgcacg ccggcggggc gttcctcagc
      601 caggccgacg aggtctgcgg tccagcgcag gagctcggcg aggacgtcct cgacggcctc
      661 agctcgctcg gcgagaaaag cctggtcaag cccgacctcg gagtcgatga ggacccgcgc
      721 ttcacgatgc tgacgacgat ccgcgactac gcccacgagc ggttggagaa ggagcaggac
      781 ttcgaacagc ttgcgcggag acacgcccac gcctatctcg ggctggtcga gggttgttcc
      841 gatcggctgc tcggccgcga gagccggcgt ctatccgacc ggctggagca ggaccatgac
      901 aacctgcgcg cagcactcga ctgggcggtt gctcatcagg aggtcgactt tgcgctgcgt
      961 ttcatggcca gtgtgtggcg cttctggcag acgcgcgggc acctcgacga ggctcgtcgt
     1021 cgagctgaga tcgtgatcgg cctgcccggt gtgcccgacc agccaaagga tctgctggca
     1081 cgcgcatacg cggcagtcgg tggcatcgct tactggcagg ccgacgtgcg cgcaacgcac
     1141 cgctactaca cgctgggact cgaagcggcg cgggcgagtg gcgatgaacg tctcgtcggc
     1201 caagcgctgt acaacctcag cttcgcgccc cttgctcagg acactctgac cagcgcgctc
     1261 tacgtagccg gacgcccaca ccttgaagac gccatcgcga tctatgagca actcggcgac
     1321 aagggtggtt tggctgacgc caagtgggcg ctctcccaag ccaccggcgc gatgggtgac
     1381 accgagggcg caatggccta cgccgtcagc gcgctggccg attaccgcga cctcaacgat
     1441 ccattccgaa cgggttgggg cctgtacatg gtcgccggac tgcgcgcggt tgggggcgat
     1501 gtgcccggca gtctctctct cctgcgcgag tcgctgtcca tcttcgccga cgctcgcgat
     1561 cgctccggaa tacttttgat cctggctggc tacgcagtcg cgagctcctt gctcggcgat
     1621 caggagttcg cggctcgaac gggcggagtt gtcgaaacgc ttcgagcgtc gaccggcgcc
     1681 ggcctgattg acgccacgcc cgaattcatt cagttccagc tgcccaaacg accggacgac
     1741 ccccacctcg ttgaagtgtg ggaggccggc gctcgcatct ctctcgaaga agccgtcgca
     1801 gagaccctgg aatggagcgc ggcagcggaa gaagccgccg ataacgccca ggtgatcgcc
     1861 gaataagccc gcgtcgtcac gatgcgcgca tgaaaagaag gatgcgcaag caccacgagc
     1921 tgggcaccag ctacggcaac ggcgtggtgg ggccgagtcg caagcaggtt cgagcggcga
     1981 ttgagacact gcccgaaaat ctgggctgga cagaactcgc cgagagcctg cgcccggtgt
     2041 tcgttcgtcg tcggccgttg ccaccgggga tcgagcggcc ggtgacgacg ctgcggccac
     2101 ccggactcga ggtggcgctg ggcgtggata tcggcccagc gttcatgtat gtcggccacg
     2161 ggatgctcga gacctggggc gttacggagg ctgacgcgtt cgagcgggcc atggcgaacc
     2221 tgcgttcgtc agtggcagcc gagaaatacg tcgagatgga atacgagtcg atcgatggcg
     2281 tgccgttctg gtggtaccag tcgcagggcg ggcttgcgtc agggctgctc ctgctggagg
     2341 acgagctggc cacccgttac ggaccggatc cgcggctcct catcgcgccg atgcgcaacc
     2401 ttctcctggc ggcccccttc gacgccgatc ggtcgcttgt ttcgtggctg cgtgacgaga
     2461 tctcgtacga cgacccaaac ggcctcgacc tcccgatctt tgccctgatt gatggccggc
     2521 tgtcgatcga cacgcgcgtg gagcggctac aggcggccgt ggttcactga gtgcgacgct
     2581 tccgcttgtc ctcacctggg atgccgcgcg cagcgaggct ggccgtgaac ccgtcgatgt
     2641 cgtccaccgc gacccaaaac gccggcggcc ggagaaggcc ccacttgggg cgctccttga
     2701 actcgagacg aacgcccccg gtgtgagctc cgacaaagct catctcgccg ttgaggatgc
     2761 cccgacgaac cccgatcgcc gtgatccaca gccacggacc ttcggcgctc cacctggcga
     2821 tgttgctcgt cggcgtgcgg aatcgaaaca tgccgaaatg agcgtcgagc tcgtcgttaa
     2881 ggtcgacgta ggagttggat gcccgagccc cccacagaag gagcagcggc cgcgactttc
     2941 ggccgaggcg catcggaaag cgggtactca attcagaact gcgcggagcg tcatcatctg
     3001 cctgccgcgg gaacgaagtg gtcggggcga gaggatttga acctccgact tcatcctccc
     3061 gaaggatgcg cgctaccagg ctgcgctacg ccccgacgtg ggaccgcgca acgaagcaac
     3121 cgtgcgcggc ccgcgagtat agacgagccc cttcagcgcc ccaaaagcag agccg
//
LOCUS       DATLCO010000151         3752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1693442, whole genome shotgun sequence.
ACCESSION   DATLCO010000151 DATLCO010000000
VERSION     DATLCO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693442"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2169
                     /gene="alaS"
                     /locus_tag="VMZ33_03755"
     CDS             <1..2169
                     /gene="alaS"
                     /locus_tag="VMZ33_03755"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HUR16376.1"
                     /translation="CIPDHDENCPRWLEIWNLVFMEFDQKPDGPRVPLPFKSVDTGMG
                     FERLTSVIQGVNTNYDTDCFTPIHEHMRAMLGHDPEGFEAERFSYQVIADHVRALTFL
                     VPDGIRPSNEGRGYVMRRLLRRAVRHGRLLGRTEPFLAELSGVVIDKMKVAYPYLEEW
                     RDQIHGVIAAEERQFARTLDAGIIQFEEALIPLTSADRVVGGKTADALPDDAPVLPGD
                     VAFRLHDTFGFPIDLTVELAAEYGVRVDRPGFETALEEQRQRSRGGRKQELARHAEMS
                     GLYQEIAARTSGGVQFLGYDTTTADARVVAILRDGTEYDQLEAVEEVELRVPAQAEAE
                     IILDRTPFYAEGGGQVGDQGALRMDVGVIFTVSDTQRPVAGLIVHRGTLHGRIAVGDE
                     VSAVVDPIRRARTMRNHTGTHVLHRALRNNVGERARQAGSLVTPDYMRFDFPFDRALT
                     DEEKRSIEAEVRAVVRENKTVTPKYMSMSEAIDAGADAFFDEKYGEQVRVVFVDGFSR
                     ELCGGTHCSASGQIGSFVITGERSIGSGMRRIEALTGDAADSHLANRLALLDRVVEQV
                     GARDAESLPDRVRELQDRTKELERRVKAGGSAGQARPTDLARGAESLDGARFVAYSAP
                     FESMKELQSFARSLRAELGDGVIALALESDEPQIFVTVSDDLVARGVSAGALVSQGAP
                     VMEGRGGGRPEMAQARGSRRDRLPSALEKIREALAEQLEQ"
     gene            2177..3454
                     /locus_tag="VMZ33_03760"
     CDS             2177..3454
                     /locus_tag="VMZ33_03760"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HUR16377.1"
                     /translation="MAFWRRFLQRPESDRLSACTALDVGTEFAKALVFEIADDPQTGK
                     PKAVVRGVGRKRQGLTHMQSGTVADIAAVVDNCQVALQEAEEMAGFRSSQVIIGIAGE
                     LVKGFTSSTSRERKHPDARLTERELDALVEATQREALRDAERTIAWETGLPNVDIRLV
                     HAAVTGATIDGYSVTNPLSFQGRHVRISIFNAFAPLIHLGALQTVASMLDLELVAIVA
                     EPYAVARCMDDDQLQQGGALFIDVGGGTTDVALVRQGGIDGTRMFALGGRAFTKSLAD
                     RLELPFARAEQIKVDFARGVKIDRAAEVAEIVAEDIAVWSAGVELVLEEFGKSGMLPG
                     RIYVCGGGARLPQIAAALRDPEFARHLPFARPPQVEAITVNEVTQIDDATGLLVDEQD
                     VPPMALAHQALEMAATESVLDASLRRVLRTMKV"
     gene            3459..>3752
                     /locus_tag="VMZ33_03765"
     CDS             3459..>3752
                     /locus_tag="VMZ33_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16378.1"
                     /translation="MSLLYLELDDEITGAIARLRAVTDGGAVMVVPAGSRIATSRINF
                     KLLAKEAADRRLNVVAVSDEPSVRALAISAGLPAYDTVANGEHALATFREQDRR"
BASE COUNT          636 a         1233 c         1265 g          618 t
ORIGIN      
        1 tgcatccccg accacgatga gaactgcccg cgctggctcg agatctggaa cctcgtcttc
       61 atggagttcg accagaagcc ggacggtccc cgtgtcccgc tgcccttcaa gagcgtcgac
      121 acgggcatgg gcttcgagcg cctgacgagc gtgatccagg gcgtcaacac caactacgac
      181 accgactgct tcacgcctat ccacgagcac atgcgggcaa tgctcgggca cgatccggaa
      241 ggcttcgagg ccgagcggtt cagctaccag gtcatcgccg atcacgttcg agcgctgacc
      301 ttcctggtgc cggatggcat ccggccgtcg aacgaaggcc gcggctacgt gatgcggcgg
      361 ctccttcgac gcgcggtgcg gcatggccgc ctgctcggtc gcacggagcc gttcctggcc
      421 gagctgtcgg gcgtcgtgat cgacaagatg aaggttgcct acccgtacct cgaggaatgg
      481 cgcgatcaga tccacggcgt tatcgcggcg gaggagcgcc agttcgcgcg cacgctcgac
      541 gccggcatca tccaattcga ggaggcgctg attccgctca cctcggcgga tcgcgtcgtt
      601 ggtggcaaga cagcggacgc acttcctgat gatgcgccgg tcctgcccgg cgacgtcgcc
      661 tttcggctgc acgacacatt tggcttcccg atcgacctaa cggtcgagct ggcggccgag
      721 tacggcgtgc gtgtcgaccg accagggttc gaaacggcgc tcgaggagca acgccagcgc
      781 agccgcggcg ggcgcaagca ggagctggcg cggcacgccg aaatgagcgg gctctaccag
      841 gagatcgccg cgcggacctc cggcggtgtg caattcctcg gctatgacac aaccacggcc
      901 gacgccaggg ttgtggccat cctgcgtgac ggaaccgaat acgaccagct ggaggcggtc
      961 gaggaggtcg agctgcgtgt gccggcgcag gctgaggcag agatcatcct cgatcgaacc
     1021 cctttctatg ccgagggcgg cggtcaggtc ggtgatcagg gcgccctgcg gatggacgtg
     1081 ggcgtgatct tcaccgtctc tgacacccag cggcctgtgg ccggattgat cgtccatcgc
     1141 ggcaccctgc acggacgaat cgcggttggt gatgaggttt cggccgtggt cgatcccatc
     1201 cgtcgcgcac ggacgatgcg caaccacacg ggcacccacg tcctgcaccg cgccctgcgc
     1261 aacaacgtcg gcgaacgtgc ccggcaggcg ggctcgcttg tcacccccga ctacatgcgc
     1321 ttcgacttcc cgtttgaccg agctctgacg gacgaggaaa aacgctcgat tgaggctgag
     1381 gttcgggccg tcgtccgcga gaacaagacg gtcacgccca agtacatgtc catgtctgag
     1441 gcaattgacg ctggcgccga cgccttcttc gatgaaaagt acggcgagca ggtccgcgtc
     1501 gtcttcgtcg atgggttctc gcgtgagctc tgcggcggga cacactgttc ggcatctggc
     1561 cagatcggct cattcgtcat caccggcgaa cggagcatcg gctctggcat gcgccgcatc
     1621 gaggcgctga ccggtgatgc cgccgattcg catctggcca accgcctggc gctcctcgac
     1681 cgggtcgtcg agcaggtcgg cgcgcgcgac gcggagtcgc tgccggatcg ggtcagggag
     1741 ctgcaggatc gaacgaagga gctcgaacgg cgagtcaagg ccgggggtag cgccggtcaa
     1801 gcacggccga ccgacctggc gcgtggcgcc gagtccctcg acggggcccg cttcgtcgcc
     1861 tactcagctc ccttcgagtc catgaaggag ctccagagct tcgctcggtc ccttcgggcc
     1921 gagctgggtg acggggttat cgcgctggcg ctcgagtcgg atgagccgca gatcttcgtc
     1981 acggttagcg acgacctcgt tgcccgcggc gtttccgccg gcgcgctggt cagccagggc
     2041 gcaccggtga tggagggtcg tggcggaggt cgaccggaga tggcgcaggc tcgaggttcg
     2101 cggcgcgacc ggctgcccag cgcactcgaa aaaattcgcg aggcactcgc cgagcagctc
     2161 gagcagtaga ccgcgcatgg ccttctggcg gcgcttcctg caacgccccg aaagcgaccg
     2221 tctgtcggcc tgcacggcgc tcgacgtcgg gacggagttc gccaaggcgc tggtgttcga
     2281 gattgccgac gacccccaaa ccggcaagcc caaggccgtc gtgcgcggcg tgggacgcaa
     2341 gcgccagggt cttacccaca tgcagtcggg cacggtcgcc gatatcgccg cggtggtcga
     2401 caactgccag gtcgcgctcc aggaagcgga ggagatggcc ggcttccgct cgagccaggt
     2461 gatcatcggc atcgccgggg agctggtgaa aggatttacc tcgagcacca gccgggagcg
     2521 caagcacccc gacgcgcggt tgaccgaacg agagctcgac gcgctcgtcg aggccactca
     2581 acgcgaggca ctgcgcgatg ccgagcgaac gattgcctgg gagaccggcc tcccaaacgt
     2641 tgacatcaga ctggtccacg cggcagtcac cggcgcgact attgacggct attcggtcac
     2701 gaacccgctc agcttccagg gccgccacgt ccggatcagc atcttcaacg cattcgcgcc
     2761 gctcatccac ctgggcgcgc ttcagaccgt ggcgagcatg ctcgacctcg agctcgttgc
     2821 gatcgtcgct gagccatatg ccgtggcacg gtgcatggac gatgaccagc tacaacaggg
     2881 gggcgcgctg ttcatcgacg tcggcggtgg caccaccgac gtggccctgg tacgccaggg
     2941 cggcatcgac gggacgcgca tgttcgcact cggcggacgg gcgttcacca agagcctcgc
     3001 cgatcgcttg gagctgccct ttgcccgcgc ggagcaaatc aaggttgact tcgcacgggg
     3061 cgtcaagatc gaccgcgccg cggaagtggc tgagatcgtg gccgaagata tcgccgtgtg
     3121 gtcggccgga gtcgagcttg tcctggagga gttcggcaag tcggggatgc tgcccggccg
     3181 catctacgtg tgcggtggcg gcgcgcgcct gccccagatt gcggccgcgc tgcgcgatcc
     3241 ggaattcgct cgccacctgc ctttcgcacg accgccgcag gttgaggcca tcaccgtcaa
     3301 cgaggtcacc cagatcgacg atgccacagg cctgttggtc gacgagcagg atgtcccgcc
     3361 gatggctctt gcacaccagg cgctcgagat ggccgccacg gagtcggtcc tcgacgcgtc
     3421 ccttcggcgt gtgttgcgca ctatgaaggt ctaggaagat gtcgctcctc tacctcgagc
     3481 tcgacgacga gatcaccggc gccatcgcac gcctccgtgc cgtgaccgat ggcggggcgg
     3541 tcatggttgt gcccgccggc tcgcgcatcg cgaccagccg catcaacttc aagcttctcg
     3601 ccaaggaagc tgccgatcgg cgtctgaacg tggtcgcggt gagcgacgag ccgtcggtgc
     3661 gtgccctggc aatctccgcg gggctgccgg cgtacgacac cgtggccaac ggcgaacatg
     3721 cattggccac cttccgggaa caggatcgtc ga
//
LOCUS       DATLCO010000152         4824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8299240, whole genome shotgun sequence.
ACCESSION   DATLCO010000152 DATLCO010000000
VERSION     DATLCO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8299240"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VMZ33_03770"
     CDS             <1..352
                     /locus_tag="VMZ33_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HUR16379.1"
                     /translation="GAREGGGAVVARVDSERLERFDQELRDLARRLEAAEARGERSLH
                     RIGVVRYNPFPDTGSNQSFVLAVLDAKSDGFVMSSLHSRQQTRVFLKALAAGRSDSAL
                     SKEETEAIDRATAR"
     gene            399..3272
                     /locus_tag="VMZ33_03775"
     CDS             399..3272
                     /locus_tag="VMZ33_03775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HUR16380.1"
                     /translation="MAVEIASPRGRPEAIVAQLNAPVPFHVAAGLLRGLNREQRRAVT
                     NRDGPQVVIAGPGTGKTEVITRRVAWLIATKRARPREILALTFTDNAAREMQARVDLL
                     VPYGQADAAIHTFHAFGDRLLREHAFELGLAGDMRLINRSEAIVLLREHLFELGLNRY
                     RPLGDPTRFLGALADAFGRAKDDGLEPSGLDADTDESAEVLAAFSRYQELLSQNGLID
                     HGDQISLALRLLRDHPQVRDQVAAQFRFVLVDEFQDMNRAQVELVSLIGHPSGNVTVV
                     GDPDQAIYRFRGASEGSLEAFENLFPDANKVVLRRNYRSRAPIVAAAQRLIEHNRHSD
                     EAQDGQVAHRRTRRELPVRALSFGSASDEADGVAASIAGAIKEGRPARAFAVLARTNG
                     EVDSIARALRARDVAVRTRQPADLFSHPAVRPLLAFLRVVADPSNTVELYALATSWPF
                     EIEGQALTTALGNCRRRHRSLWDELRSAPDHVGDGSVAAGTERLIAHVEAAVERSHAR
                     SCGEVLYDHIRQTGLLRKLQSAVSSDDARAVARFFEVVRSRASLLNLDRVAVLVPHLD
                     ALLEASDDDYLEHDTPDADAVTVLTVHGAKGLEFDRVFLTGLVDGRFPTRSRPPLLTV
                     GPAASGAEADPLADERRLFFVAMTRARDELVLTHHVTTASGRARRPSIFIAETLDVPS
                     VPQARDGDVLARIERAAASTQGAMTVVPHDAPVTDLSFSALDDYLSCPERYRLRHVIG
                     LPTPQHHALSYGSAMHQAVAAFHTSRARGSVMTEEELLSVFRASWSVEGFVSREHEEA
                     RYAAGCAALVRFRNDQIANPADVVAVERPFTFRFGDVRIKGRLDRLDRTDEGSVIVDY
                     KSSDVSDPQKANARARESLQLQVYAMAHEAETGRAPHAMQLHFLDTGVVGTVKPEPER
                     QQKARAKIASAVDGIRAGAFEAAPTPMACGYCPYRQICASSAA"
     gene            3302..3676
                     /locus_tag="VMZ33_03780"
     CDS             3302..3676
                     /locus_tag="VMZ33_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16381.1"
                     /translation="MNRGDRWANLALFAFAAAAWIGVAVIFQTLFPQQSAATLLAGAL
                     LLGGAVTATVTPLFWLGRFVMTRRIAYRGDWWRALRRGALVGLVVTLFVVMRGMGAFS
                     MPLLLFVVAMAVLVELTLSVRS"
     gene            3783..>4824
                     /gene="speB"
                     /locus_tag="VMZ33_03785"
     CDS             3783..>4824
                     /gene="speB"
                     /locus_tag="VMZ33_03785"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HUR16382.1"
                     /translation="MSHDRLPNDYAQINGWDRPHDVYVSPDMPQYAGATVSFCRRPLI
                     ESAEALAARGADVAILGAPLDDGVSYRPGARFGPRAIRQANYNNGGHSLQLGVEPFEI
                     LDVVDVGDANVVPAALERGHAMIFRKVLEVARTGTVPIILGGDHSITWPSASAVAQAH
                     APKSIGIVHFDAHADTANDDWGVLAGHGAPMRRLIESGAVDGRNFVQVGLRGYWPPPD
                     VFGWMKEHGLRWHLMKEIEDRGADRVVDDAISEALDGPDAIYLSIDIDVLDPGMAPGT
                     GTPEPGGMLTRELLRAIRRVVGGVDLAAMDVVEVSPPYDHAEITAAAAHRCVLEAISA
                     LAVKKRDGKTVRF"
BASE COUNT          789 a         1575 c         1595 g          865 t
ORIGIN      
        1 cggcgcgcgc gaggggggtg gcgctgtagt cgcacgtgtt gattccgagc ggctagagcg
       61 gtttgaccaa gagttgcgcg accttgctcg ccggttggag gctgccgaag cgcgcggcga
      121 gcgatcgctc caccgcatcg gtgtggtccg ctacaaccca tttcccgaca ctggctccaa
      181 ccagagcttc gtcctggccg tcctagatgc caagagcgac gggttcgtga tgtcaagcct
      241 gcactccaga cagcagactc gggtcttcct gaaggcgctc gccgcgggca ggagcgactc
      301 ggcgctgtcg aaggaagaaa cagaagcgat cgatcgcgcg acggcgcgct aaccgtgtgc
      361 cagcctagct ggcacaacgt ctcccgaata ttcgggtcat ggcagtcgag atcgcgtcac
      421 cgcgagggcg tcctgaagca atcgttgcgc aactcaacgc gccagtgccg ttccacgttg
      481 cggcggggct gctcagaggg ttgaaccgcg agcagcgtcg ggcggtgacc aaccgcgacg
      541 gaccgcaggt cgtgatcgcc gggcctggca cgggcaaaac ggaggtcata acgcgccggg
      601 tggcatggtt gatcgccacc aaacgtgcgc ggccgcggga aatcctggcg ctcactttca
      661 cggacaacgc cgcccgtgag atgcaggcac gggttgatct gctggtcccc tacggtcagg
      721 cagacgcggc cattcacacg ttccacgcgt tcggtgatcg gctcctgcgt gagcacgcgt
      781 tcgagctcgg tctagccggt gacatgcggc tgatcaatcg ttccgaggcg attgtgctgc
      841 tgcgcgagca cctctttgag ctgggcctga acaggtaccg gccgctcggc gatccgacgc
      901 gatttctcgg cgcgctggct gacgcattcg gccgcgccaa ggacgatggg cttgagccat
      961 ccgggttgga tgcggatacg gacgagagtg cagaggttct cgccgctttc tcgcgctacc
     1021 aggagcttct gtcccaaaac gggttgattg accacggcga ccagatcagc ttggcgctgc
     1081 gacttctgcg cgaccacccc caggtcaggg accaggtcgc tgctcagttc cggttcgtgc
     1141 tcgtcgacga gtttcaggac atgaaccgcg cccaggttga gctcgtgtcg ctgatcggcc
     1201 acccatcggg caacgtaacg gtcgtcggcg acccggacca ggccatctat cgcttccgtg
     1261 gcgcgtccga aggcagcctc gaggccttcg agaatctctt tccggacgcc aacaaggttg
     1321 tgctgcggcg caactaccgg tcacgagctc cgatcgtggc tgcggcccag cggttgatcg
     1381 agcacaaccg ccattcggac gaagcccagg acgggcaagt cgcccaccga cggacgcggc
     1441 gcgagctccc ggtccgtgcc ttgagcttcg ggtcagcgag tgacgaggcc gacggcgtgg
     1501 ccgcgagcat cgccggggcg atcaaagagg ggcgcccagc ccgggcattt gctgtcctgg
     1561 cacgaacgaa cggagaggtc gattcgattg cgcgagccct gcgcgcgcgt gacgttgcag
     1621 ttcggacacg ccagcctgct gatcttttct ctcatccggc cgttcgacca ttgctcgcct
     1681 tcctgcgcgt cgtcgcagac ccttccaaca ctgtcgagct ttacgcgttg gcaaccagct
     1741 ggccgttcga gatcgaaggg caggcgctaa cgactgcgct tggcaactgc cgtcgccgcc
     1801 accgaagcct ctgggacgag cttcgctctg cgcccgacca tgtcggcgac ggcagcgtgg
     1861 cggccgggac tgaacggctc attgcccacg tcgaggccgc agttgagcgg tcgcatgcgc
     1921 gttcctgtgg cgaggtcctc tacgaccaca tccgccagac agggctgctg cgcaagctgc
     1981 agtcggccgt gtcctcggat gacgcgcgcg ctgtagcccg gttcttcgag gtcgtccgtt
     2041 cgcgggcgtc gctgctgaac ctcgatcgcg tcgcagtcct cgtcccacac ctcgacgcgc
     2101 tgctggaagc cagcgacgac gactatctcg agcacgacac gcctgacgcc gatgccgtca
     2161 ccgtgctcac cgtgcacggg gccaagggcc ttgagttcga tcgggtcttt ctgacaggcc
     2221 tcgtcgacgg cagattcccg acccgaagcc gaccgccgct gctgacggtt gggccggccg
     2281 cttcgggcgc cgaggccgat ccgctcgctg atgagcgccg actcttcttc gtagcgatga
     2341 cgcgagcccg cgatgagctg gtcttgacac atcacgtgac cacggcaagt ggacgtgctc
     2401 gccgtccgtc aatcttcatc gctgagacgc tggacgtccc ctccgtgccc caggcgcgcg
     2461 acggcgacgt cctcgcccgc atcgagcgcg ctgccgcctc gacgcaaggt gcaatgaccg
     2521 tggtgcccca cgacgcgccg gtgacagatc tcagcttctc agcgctcgac gactacctca
     2581 gttgtcccga gcgttatcgg ctgcggcacg tgattgggtt gccgactccc cagcatcacg
     2641 cgctctcata cggcagtgcc atgcatcagg ctgttgccgc tttccacacg agtcgcgctc
     2701 gagggtccgt catgactgag gaggaactgt tgagcgtgtt ccgcgcctcg tggtcggtcg
     2761 aggggttcgt ctcgcgcgag cacgaagagg ctcgatacgc cgccggatgc gcagccctgg
     2821 tgcggttccg gaacgaccag attgcaaatc cggcagacgt cgtagcggtt gagcgcccgt
     2881 tcaccttccg cttcggggac gtgcggatca agggccgcct cgaccgcctc gaccgcaccg
     2941 atgagggatc agtaatcgtt gactacaagt catccgacgt cagtgaccca cagaaagcga
     3001 acgcccgggc gcgcgaatcc ctgcaactgc aggtgtacgc gatggcgcac gaagccgaaa
     3061 cgggacgagc cccgcacgcc atgcagctgc actttctgga cacgggggtc gttggcaccg
     3121 ttaagccgga accggagcga cagcaaaagg cccgggccaa gattgcaagc gccgtggacg
     3181 gcatccgcgc cggagcgttt gaggctgcgc cgaccccgat ggcctgcggc tactgccctt
     3241 accgacagat ctgcgcgtcg agcgcggcgt gactcagttg gccgggctat cgtggccggc
     3301 agtgaaccgc ggcgatcgat gggcgaactt ggcgctcttc gcgttcgcgg ctgcggcgtg
     3361 gatcggcgtc gctgtcatct ttcaaacctt attcccgcag cagagcgcgg ccacgctgct
     3421 ggctggtgcg ctgctcctgg gcggcgctgt cacggccacg gtgaccccgc tgttttggct
     3481 cggacggttt gtgatgaccc gcagaattgc gtaccgcggt gattggtggc gggcgttgcg
     3541 tcgaggggca ctggtcggtc tcgtggtgac gcttttcgtc gtaatgcgtg ggatgggcgc
     3601 gttctccatg ccactcttgt tgttcgtcgt agcgatggcc gttctcgtgg agctgaccct
     3661 gtccgtgcgc tcgtaggacg ttcgtgccaa cgcacatttg gcggtcaagc gtaggattca
     3721 gcagggcgcc ctgctggacc gcggcacgga accggcggcg actcgcgcgt gaggaccgtg
     3781 cgatgagcca cgatcgcctg ccgaacgact atgcgcagat caacggatgg gaccgcccac
     3841 acgacgtcta cgtctcccct gatatgcccc aatacgcggg cgccacagtc agcttttgcc
     3901 gtcgcccgct gatcgagtct gcagaggcgc tcgccgcacg aggtgccgat gtggcgatcc
     3961 ttggagcgcc gcttgacgac ggagtcagct atcggccggg cgctcgcttt gggccacgcg
     4021 cgattcgcca ggcgaactac aacaacggcg gccactcctt gcagctcggt gtcgagccgt
     4081 tcgagatcct tgacgtcgtc gacgtgggcg acgccaatgt cgtgccggcg gcactggaaa
     4141 gaggccacgc gatgatcttc cggaaagtgc ttgaggttgc gcgcaccggg acggtcccga
     4201 tcatcctggg cggtgatcac tcgattacct ggccgtcggc tagcgcggtc gctcaggccc
     4261 acgcccccaa gagcatcggc atcgtccact tcgacgcgca cgccgatacc gccaacgacg
     4321 attggggcgt gctcgccgga cacggtgcac cgatgcgccg cctcatcgag tcgggcgccg
     4381 tagacgggcg caacttcgtc caggtcggtc tgcgcggcta ttggccgccc cctgacgtat
     4441 tcggctggat gaaagaacac ggcctgcgct ggcacctgat gaaggagatc gaggatcgtg
     4501 gcgccgacag ggtcgtggac gacgcgattt ccgaggccct tgacggtcct gatgcgatct
     4561 acctgtcgat cgatatcgac gtcctcgatc cgggcatggc ccccggcacc ggcacgcccg
     4621 agccgggcgg gatgctgaca cgcgagctcc tgcgcgccat tcgccgcgtc gttggcgggg
     4681 tcgatctggc ggcaatggac gtcgtggagg tctcaccgcc ttacgaccac gcggagatca
     4741 cggccgccgc cgctcaccgt tgcgtgctcg aggccatttc tgcgctcgcg gtgaagaagc
     4801 gcgacggaaa gacggttcga ttcg
//
LOCUS       DATLCO010000153         8511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9147437, whole genome shotgun sequence.
ACCESSION   DATLCO010000153 DATLCO010000000
VERSION     DATLCO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9147437"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /gene="rpsC"
                     /locus_tag="VMZ33_03790"
     CDS             <1..460
                     /gene="rpsC"
                     /locus_tag="VMZ33_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HUR16383.1"
                     /translation="VIGKGGANVEALRQQIGRMTTSKVKLEIKEIRQPELDAVLVASN
                     IAQQLTRRIAFRKALKQSVQRTMKAGAKGVRIAVSGRLGGSEMARREWDKEGRIPLNT
                     LRADISYGQVHAHTTYGRIGVKVWIYRGDVMPERQAPPESAAATAAAAGA"
     gene            465..899
                     /gene="rplP"
                     /locus_tag="VMZ33_03795"
     CDS             465..899
                     /gene="rplP"
                     /locus_tag="VMZ33_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HUR16384.1"
                     /translation="MLMPRRVKHRRVQRGRMSGLAKGGNTIAFGEFALMTEEPAWITA
                     RQIEAARRAMTRYVKRGGKIWIRIFPDKPVTKKPAEVRMGSGKGAPDHWVAVVKPGRI
                     LFEMAGVPQVEAVEAMRLASHKLPINVKFLVREGVGGGDGHR"
     gene            886..1257
                     /gene="rpmC"
                     /locus_tag="VMZ33_03800"
     CDS             886..1257
                     /gene="rpmC"
                     /locus_tag="VMZ33_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HUR16385.1"
                     /translation="MDIAKVRELNDDELGNELIATRRHLYDLRFQLATRQLTDHSQLT
                     QARRTIARVLTVMTERGIDENKIRPLPQPRRAATRGAATRGRGRSTSDKAPAKTTAAR
                     TAAKATDAKATASKATGGNDE"
     gene            1314..1541
                     /gene="rpsQ"
                     /locus_tag="VMZ33_03805"
                     /pseudo
     CDS             1314..1541
                     /gene="rpsQ"
                     /locus_tag="VMZ33_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            1689..2057
                     /gene="rplN"
                     /locus_tag="VMZ33_03810"
     CDS             1689..2057
                     /gene="rplN"
                     /locus_tag="VMZ33_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HUR16386.1"
                     /translation="MIQQQSRLKVADNTGARVVQVIRVLGRSQKTTAGIGDVIIASVK
                     QAIPNAAVKKGDVVRAVVVRTNKEVGRPDGSYIRFDENAAVLINNQGNPRGTRIFGPV
                     ARELRDGGYMKIVSLAPEVL"
     gene            2057..2521
                     /gene="rplX"
                     /locus_tag="VMZ33_03815"
     CDS             2057..2521
                     /gene="rplX"
                     /locus_tag="VMZ33_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HUR16387.1"
                     /translation="MVKAIAPQRSPRVPDIRRGDEILVLKGKDAGKRGTVEHVVRNSQ
                     SFKKTISKYGSNWRKVSPLASVAVVVEGMNIAKRHTKPRPKSGRTERQPRIQQGGILE
                     VPQPILASKVMVICPHCGQPTRIKHGPGPDGRSIRICSNCGEAVTREAAAKK"
     gene            2851..3390
                     /gene="rplE"
                     /locus_tag="VMZ33_03820"
     CDS             2851..3390
                     /gene="rplE"
                     /locus_tag="VMZ33_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HUR16388.1"
                     /translation="MRGRYHSDVVPALQKEFGYANPMQVPRLSKIVVNIGLGEALSNA
                     KALDAAVGDLASITGQKPIVTRARRSIAQFRLRTGNPIGAKVTLRGQQMWDFLERLTR
                     LALPRIRDFRGVPTKSFDGRGNYSLGLREQLAFPEIDYDKIDRLRGLEVSIVTTAKTD
                     EESKRLLQLLGMPFAGQNG"
     gene            3406..3591
                     /locus_tag="VMZ33_03825"
     CDS             3406..3591
                     /locus_tag="VMZ33_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HUR16389.1"
                     /translation="MAKKSLIAKQKRKPHQESQGYNRCFVCGRPRGYMRRFGLCRICF
                     RERALLGELPGVTKSSW"
     gene            3596..3991
                     /gene="rpsH"
                     /locus_tag="VMZ33_03830"
     CDS             3596..3991
                     /gene="rpsH"
                     /locus_tag="VMZ33_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010169919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HUR16390.1"
                     /translation="MNISDPIADMLTRVRNGSRARHTEVLVPASRTKRAIAEILRDEG
                     FIETFDETREGPADYLRLQLKYVGKVPVISGLKRVSKPGLRVYAAKTDIPRVLGGLGV
                     VIVSTSHGIMTGSRARKAQLGGEILAYVW"
     gene            4012..4563
                     /gene="rplF"
                     /locus_tag="VMZ33_03835"
     CDS             4012..4563
                     /gene="rplF"
                     /locus_tag="VMZ33_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HUR16391.1"
                     /translation="MSRIGRMAIPIPSGVQVSIDGNHVEVTGPKGRLERDIAPELLLV
                     NDDGTLRVERPNDDKKSRELHGLTRTLVANMVTGVTDGFRKGLEITGVGYRAQLVGKK
                     LQLNLGYSHPIEIDPPDGVTFEVENPTRLAVVGVDKELVGHIAARVRATRKPEPYKGK
                     GVRYAGEVVRRKAGKAGKIGGKK"
     gene            4560..4922
                     /gene="rplR"
                     /locus_tag="VMZ33_03840"
     CDS             4560..4922
                     /gene="rplR"
                     /locus_tag="VMZ33_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HUR16392.1"
                     /translation="MSVTPSRRAARDKRHTRIRLSLEGTQLRPRLAVFRSLNQIYAQV
                     IDDSAGRTLAAASSLETGVRGSAGNKTESAKTVGRLLAERAKGAGVKQVVFDRAGYRY
                     HGRVRALADAAREAGLDF"
     gene            4931..5425
                     /gene="rpsE"
                     /locus_tag="VMZ33_03845"
                     /pseudo
     CDS             4931..5425
                     /gene="rpsE"
                     /locus_tag="VMZ33_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            5493..5696
                     /gene="rpmD"
                     /locus_tag="VMZ33_03850"
     CDS             5493..5696
                     /gene="rpmD"
                     /locus_tag="VMZ33_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HUR16393.1"
                     /translation="MAGKLRVTLIKSPVSHTKRTRATMRAIGLHRIGETIEIDDTPPL
                     RGMANAIRFLVRVEEIKSEEVKS"
     gene            5693..6463
                     /gene="rplO"
                     /locus_tag="VMZ33_03855"
     CDS             5693..6463
                     /gene="rplO"
                     /locus_tag="VMZ33_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HUR16394.1"
                     /translation="MKLHDLRPAAGSRKARTRVGRGIAAGQGKTAGRGTKGQRARAGA
                     SIPAWFEGGQTPIHIRVPKLRGFKNRFKIEYQPVNVGVISAYAEAGRFGVEGHAKEDV
                     ITVNAETLASAGLISSAEKPIKVLGQGEISQKVFVAADKFSTSARAKIEAAGGFVQVL
                     APEKAEKTSKAERTAAADRRRRGDEPAGSADAGGSRDAPESEGSATSTLSSGQAAADL
                     GGDESPESGGSTASEIASEPEAAAKPAKRAAKKDTAGS"
     gene            6553..7854
                     /gene="secY"
                     /locus_tag="VMZ33_03860"
     CDS             6553..7854
                     /gene="secY"
                     /locus_tag="VMZ33_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HUR16395.1"
                     /translation="MFEALINAFRAPDIRRRILFVLGMLVVYRALAVVPVPAVDQEAV
                     RGLIESQTFLQLLDLFSGGGLSSFSIVAMGVNPYINASIIMQLMTGVVPRLQALSREG
                     EYGRTRINQYTRYLTVPLALLQAYGYLAILSASQIVTEFDILSIQTIGQMVTLAAGTV
                     LAMWIGELITERGIGNGISFIIFAGIVARVPQGVASFLSAPDIAAGVAFVILAVVVVA
                     VIVYIQEGQRRIPIQYASRVRGRRMYQGGATFLPLRVNQAGVIPIIFAVSILLFPTQI
                     ASYFATPPGTEPGQITLMNQIADGIVGFLNPNGPAYVIGYFLLTMGFTYFYTAFTFKP
                     DETADQLRKNGGFIPGIRPGAPTRDYLAKVVFRLSLAGAIFLALIAVSPIVLGQVFQG
                     GVVGGFALGGTALLIVVSVVVETMKQIEAQLMMRNYEGFIR"
     gene            7851..>8511
                     /locus_tag="VMZ33_03865"
     CDS             7851..>8511
                     /locus_tag="VMZ33_03865"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HUR16396.1"
                     /translation="MRVYVMVGAPGAGKGTQAALLAERLGVAHVASGDLFRENIKRET
                     ALGKKVRKFLEAGALVPDDLTVQMISDRLSKPDATAGVIFDGFPRTRPQAEALDRMLD
                     KKGVEVTAALYIDVGREELVRRLSGRWLCTESSDHVYHEVARPPQKAGVCDFDGAPLY
                     QRDDDKPETIAARLEKQLPPMYEVVDHYAGTDVLNTVRGDHTVDQVTDDLLHAIALPH
                     SS"
BASE COUNT         1577 a         2818 c         2800 g         1316 t
ORIGIN      
        1 cgtcatcggc aagggtggcg ccaacgtcga ggcgctgcgc cagcagatcg gccgcatgac
       61 gacatcgaag gtcaagcttg agatcaagga gatccgtcag cctgagctgg acgctgtcct
      121 cgtggcctcg aacatcgccc aacagctgac gcgccgcatc gcgttccgca aggcgctcaa
      181 gcagtccgtg cagcgcacga tgaaggccgg tgccaagggt gtccgcatcg cggtctccgg
      241 gcgcctcggt ggttccgaga tggcccgtcg cgaatgggac aaggaaggcc gcatcccgct
      301 gaacacattg cgtgccgaca tcagctacgg ccaggtccac gcgcacacca catacggccg
      361 catcggcgta aaggtctgga tctatcgcgg cgacgtcatg cccgagcggc aggctccgcc
      421 tgagtcggca gcggccactg cggccgcggc aggagcgtaa cgacatgttg atgccccgtc
      481 gcgtcaagca ccgccgcgtc caacgtgggc gcatgtccgg cctggccaag ggcggcaaca
      541 ccatcgcctt cggcgagttc gcgctcatga ccgaggagcc ggcgtggatc accgcgcgac
      601 agatcgaagc cgctcgacgc gccatgacgc gctacgtcaa gcgtggcggc aagatctgga
      661 tccgcatctt tcccgacaag ccggtcacca agaagccggc cgaggttcgc atgggctccg
      721 gcaagggcgc gcccgaccat tgggtcgctg tcgtcaagcc tggccgcatc ctgttcgaga
      781 tggccggtgt ccctcaggtc gaggccgtcg aggcgatgcg actcgccagc cacaagctgc
      841 cgatcaacgt caagttcctt gtgcgagagg gtgtaggtgg tggcgatgga catcgctaag
      901 gttcgcgagc tcaacgacga cgagctcggt aatgagctca tcgcgacgcg acgccatctg
      961 tatgacctgc ggttccagct ggccacgcgc cagctgaccg atcactcgca gctgacgcag
     1021 gcgcggcgaa ccatcgcgcg tgtgctcacc gtgatgacgg aacgcggcat cgacgaaaac
     1081 aagattcggc cactgccaca gccgcgccgt gcggcgacgc gtggcgctgc cacgcgtggt
     1141 cgcggtcgct cgacgagcga caaggcaccc gcaaagacaa cggccgcacg caccgccgcc
     1201 aaagcgacgg atgccaaggc tacggccagc aaggccaccg gaggtaatga cgaatgagcg
     1261 cccaggcgct gcgcaccgag gcgacgccgg cccgcatggg tcgcccgcag ggtatgcgca
     1321 aggaaaaggt cggacgcgtg gtgtccgaca agatggacaa gacaatcgtc gtctcggtcg
     1381 agcgcctgca gcggcacccg ctctacaagc gcgttgtccg gctgacgacg aagttcaagg
     1441 cccatgacga gcgcaacgag gcgcgcatcg gtgacaccgt gcgtatcgtc gagtcgcgcc
     1501 ccttgtcggc gaccaagcgc tggcgcatgg tggagatcgt cgcccgcgcg ggcgaaggtg
     1561 ccaatgagcc ggtcgtcgcc gaggaagagg caacctcaga ggccattcac atggccgctc
     1621 accccggccg cgagcgcgtc gacacagacg cggccgctgg cgcggatgag gcggaggggg
     1681 ctgcctaagt gatccagcag cagagccgtc tgaaagtggc cgacaacacc ggtgcccgcg
     1741 tcgtgcaggt gatccgcgtg ctcggccgct cgcagaagac gactgccggc atcggcgacg
     1801 tgatcatcgc gtcggtcaag caggcaatcc cgaacgccgc cgtcaagaag ggcgacgtcg
     1861 ttcgcgcggt cgtcgtgcgc accaataaag aggttgggcg cccggatggc tcgtacatcc
     1921 gttttgacga gaacgccgcg gtcctcatca acaaccaggg caacccgcgc gggacgcgca
     1981 tcttcggccc cgtggcacgc gagctgcgtg acggtgggta catgaagatc gtctcgctgg
     2041 caccggaggt gctgtgatgg tgaaagcaat cgccccccag cgctccccgc gagtgccgga
     2101 tatccggcgt ggcgacgaga tcctcgtcct caaaggcaag gatgccggca agcgcggcac
     2161 tgtcgagcac gtcgtacgca actcgcagag cttcaagaag acgatcagca aatacggctc
     2221 caactggcgc aaggtttcgc cgttggccag cgtcgccgtc gtcgtcgagg gcatgaatat
     2281 tgccaagagg cacaccaagc cgcgcccgaa gtcgggccgc acggagcgcc agccacgcat
     2341 ccagcagggc ggcatcctcg aggtgccgca gccgatcctg gccagcaagg tcatggtcat
     2401 ctgcccgcac tgcggccagc cgacgcgcat caagcacggt cccggcccag acggtcgatc
     2461 gatccgcatc tgcagcaatt gcggcgaagc ggtgacgcgc gaggcagcgg ccaagaaatg
     2521 accgaagaag tgaccagaga cactgatatg acagaagacc agcccacctc gacaaccgaa
     2581 gaaggcaccc agtccgcgga atccaccgaa accgaggagc aggtggctgc gaccgcggcg
     2641 ggcgaggagt cgaccgaagc cgcgcctgcc gacgagacgg ctgaggtgtc gacgaccgag
     2701 gccgcgcctg ccgagacgac cgaggccacg accgaagcgc cgaccgaaga tgcccgtcgc
     2761 gcgacaccgc ggtcacgcgg caagcgccaa cccgcggccg aggaggcctc ggtgtcggca
     2821 gcggcggccc ccaacaacgg cgacccgggc ctgcgcggcc ggtaccacag cgatgtggtg
     2881 cccgcgctgc agaaggagtt cggctacgcc aacccgatgc aggtgccgcg cctttccaag
     2941 atcgtcgtga acatcggcct cggtgaggcg ctctcaaacg ccaaggcgct cgatgccgcg
     3001 gtcggcgatc tggcgtccat caccggacag aagccgatcg ttacgcgcgc gcggcgctcc
     3061 atcgcccagt tccgcttgcg gacgggcaac ccgatcggcg ccaaggtgac gctgcgcggt
     3121 cagcagatgt gggactttct ggagcgtttg acgcgcctgg ccctgccccg catccgcgac
     3181 ttccgtggcg tgcccaccaa gtcgttcgac ggtcgcggca actactcact cgggctgcgc
     3241 gagcagctcg cgttccccga gatcgactac gacaagattg accggttgcg tgggctcgag
     3301 gtgagcatcg tcacgaccgc caagaccgac gaggaatcaa agcggctgct gcagctgctc
     3361 ggcatgccat ttgccgggca gaacgggtag ctgacaggag tatcgatggc taagaaatca
     3421 ctcatcgcga agcagaagcg caagccgcat caggagtcgc agggctacaa ccggtgcttc
     3481 gtctgtggtc gcccgcgcgg ctacatgcgc cgcttcggcc tgtgccgaat ctgcttccgc
     3541 gagcgtgcgc tgctgggcga gctgcccggc gtgaccaagt cgagctggta gtccaatgaa
     3601 tatctctgat cccatcgccg acatgctcac gcgtgtccgc aacggcagcc gcgcgcgcca
     3661 cactgaggtt ctcgtcccgg cgtcgcgcac taagcgcgcc attgccgaga tcctgcgtga
     3721 cgaaggtttc atcgagacct tcgacgaaac ccgcgagggt ccagccgact atctgcgcct
     3781 ccagctcaag tacgtcggca aggtcccggt catcagcggg ctgaagcgcg tcagcaagcc
     3841 cggcctgcgc gtgtatgcgg ccaagaccga catcccgcgc gttctcggcg gccttggcgt
     3901 cgtcatcgtc agcaccagtc acggcatcat gaccggctcg cgtgcccgta aagctcagct
     3961 cggtggcgag atcctcgcct acgtctggta gatcgataga gcattagaga aatgtcccga
     4021 atcggccgta tggccatccc tatcccgagc ggcgtccagg tcagcatcga cggcaaccac
     4081 gtcgaagtga ccggccccaa aggcaggctc gagcgcgaca tcgcgccgga gctgctgctc
     4141 gtcaacgacg atggaacgct ccgcgtggag cggcccaacg acgacaagaa gtcgcgcgag
     4201 ctgcacggtc tgacccgaac gctcgtggca aacatggtta cgggcgtgac cgacggcttc
     4261 cgcaaggggc ttgagatcac cggcgtcggc taccgcgcgc agctcgtcgg caagaagctc
     4321 cagctcaacc tgggctactc gcacccgatc gagatcgatc cgcccgacgg cgttaccttc
     4381 gaggtagaga acccgacccg cctcgcggtc gtcggcgtcg acaaggagct cgtcggtcac
     4441 atcgccgcgc gcgtgcgcgc tacccgcaag cctgagccct ataagggcaa gggcgtgcgc
     4501 tacgcgggcg aggtcgttcg ccgcaaggcg ggcaaggcgg gcaagatcgg aggcaagaag
     4561 tgagcgtgac cccaagccgt cgcgcagcac gtgacaagcg ccatacccgg atccgcctgt
     4621 cgctggaggg cacgcagctg cgcccgcgcc tggcagtgtt ccgcagcctg aatcagatct
     4681 atgcccaggt catcgacgac tcggccggcc gcacgcttgc ggctgcctcg tcgctcgaga
     4741 cgggcgtccg tggctcggcc ggtaacaaga ccgagagcgc caagaccgtc ggccgtcttt
     4801 tggccgagcg ggccaagggc gccggcgtta agcaggtcgt gttcgaccgc gccggctacc
     4861 gctatcacgg ccgagtacgc gcgctggctg acgccgcgcg tgaagccggc ctggacttct
     4921 aggagacaca gtggccagga tcgacccagc ccgactgacg ctcgaagagc gcgtcgtcca
     4981 gatcaatcgc gtcgccaagg tggtcaaggg cggccgccgt ttctcgttct ctgcggtcgt
     5041 cgtcgtcggt gacggcgccg ggcacgttgg cgcgggtctg ggcaaggccg gcgaggttcc
     5101 cgaggcgatt cgcaagggcg tcgaggacgc gaagaagaac atcatcaagg tacccatggt
     5161 cgggacgacc atcccgcatg aggtcacgca gtcgtttggt gcctcaaagg ttctgctgaa
     5221 gcccgcttcg cagggcaccg gcgtcatcgc cggcggctcc gtgcgtgccg tcgtcgaggc
     5281 agcgggcatc cgcgacatcc tgtcgaagtc gctcggctcg accaacccgg tcaacgtcgt
     5341 ccgcgcaacg cttgatgcgc tgcgctcgat gcactccgcc gaggagctcg gtcagcggcg
     5401 cggcaaggtg ctgcgcagca tgatcagcgg ccagcccatg tcgggcggca acaacggcca
     5461 gggttcaggc ccagccgcgg aggtgccaag cagtggccgg taagctgcgc gtgacgctca
     5521 tcaagagccc cgtttcccac accaagcgga ctcgcgcgac gatgagggcc atcggcctgc
     5581 atcgcatcgg cgagacgatc gagatcgatg acacgccgcc tctgcgcggg atggccaacg
     5641 ccatccgctt cctggtacga gtcgaagaga tcaagtccga agaggtcaag tcgtgaagct
     5701 ccatgatctg cgcccagccg ctggatcgcg caaggcacga acgcgcgtcg gccgcgggat
     5761 cgccgccggc cagggcaaga cggccggccg cggcaccaag ggccagcggg cacgcgcagg
     5821 agcgagcatc cctgcctggt ttgaaggcgg tcagacgccg atccatattc gcgttcccaa
     5881 gctgcgcggc ttcaagaacc gcttcaagat cgagtatcag ccggtcaacg tcggcgtgat
     5941 ctcggcctat gccgaggccg gacgcttcgg cgtcgagggc cacgccaagg aagacgtgat
     6001 caccgtcaat gcggagacgc tcgcttccgc gggtctcatc agctccgccg agaagcccat
     6061 caaggtgctc ggccagggcg agatcagcca gaaggtgttc gtcgccgctg acaagttcag
     6121 cacgtcggca cgcgcgaaga tcgaggcggc cggaggcttt gtgcaggtgc tcgcacctga
     6181 gaaggctgag aagacctcga aggccgagag gaccgcagcc gccgacaggc gccgaagggg
     6241 cgacgagccc gcgggctctg cggacgcggg aggtagccgc gacgcaccgg agtcggaagg
     6301 ctccgcgact tctacgctgt cgagtggaca ggcggctgct gatcttggcg gcgacgaatc
     6361 gcccgagtcg ggtggctcga cggcatcgga gatcgcttcg gagccggagg ccgcggccaa
     6421 gcccgccaaa cgggcggcca agaaggacac agccgggtct tgacttcgga aatgttcgca
     6481 acccacgtta tgtcggtgtc gtcgacatcc gctgtcgtta gcgacacctc gtcacgccga
     6541 gagggttaac taatgttcga ggcactgatt aacgcgttcc gcgcgccgga tatccgtcgc
     6601 cggatcctgt tcgtgctcgg catgctggtt gtctatcgag cactggccgt cgtgcccgtg
     6661 cccgccgtcg accaggaagc cgtgcgtggc ctaatcgagt cgcagacgtt cctgcagctg
     6721 ctcgacctgt tctcaggtgg cggcttgtcc agcttctcga tcgtggccat gggcgtgaac
     6781 ccgtacatca acgcctcgat catcatgcag ctcatgacgg gtgtcgtgcc ccgtcttcag
     6841 gcactctccc gcgagggtga gtacggccgg acgcgcatca accagtacac gcgctacctg
     6901 accgtcccgc tggcgctcct ccaggcctat ggctacctgg ccatcctttc cgcgagccag
     6961 atcgtgaccg agttcgacat cctgtcgatc cagaccatcg gccagatggt caccctcgcc
     7021 gcaggcacag tcctcgcgat gtggatcggc gagctcatca ccgagcgggg gatcggcaac
     7081 ggcatcagct tcatcatctt cgccggcatc gtcgcccgcg tgccacaggg cgtggcctcg
     7141 ttcctgtcgg cccccgatat cgctgcaggc gtggccttcg tgatcctcgc cgtggtcgtc
     7201 gtcgccgtga tcgtctatat ccaggaaggc cagcgccgca tcccgatcca atacgcgtct
     7261 cgcgttcgcg ggcggcgcat gtaccagggt ggagcgacgt tcctgccact tcgcgtcaac
     7321 caggcaggcg tgatcccgat catcttcgcc gtcagcatcc tcctgttccc gacgcagatc
     7381 gcttcgtact tcgctacgcc gccgggcacc gagcccgggc agatcacgct catgaaccag
     7441 atcgcggacg ggatcgtcgg cttcttgaat ccgaacgggc ccgcgtacgt gatcggctac
     7501 ttcctgctga cgatgggttt cacctacttc tatacagcgt tcaccttcaa gcccgatgag
     7561 acggccgacc agctgcgcaa gaacggtggc ttcattccag gcatcagacc cggagcgccc
     7621 acgcgtgact acctggccaa ggtcgtgttc cgcctgagcc ttgcgggggc catcttcctg
     7681 gcactcatcg ccgtttcgcc gatcgtcctc ggccaggtgt tccagggagg cgtggtgggc
     7741 ggattcgccc tcggcgggac ggccctgctg atcgtggtca gtgtcgtggt cgagacgatg
     7801 aagcagatcg aggcccagtt gatgatgcgc aactacgagg gcttcattcg ttgagggttt
     7861 acgtcatggt cggggcgccg ggtgccggaa agggcacaca ggcggcgctc ctggccgagc
     7921 ggctgggcgt tgcccacgtc gcctcgggcg atctcttccg cgagaacatc aagcgcgaga
     7981 cggcgctcgg caagaaggtc aggaagttcc ttgaggccgg cgccttggtg cccgacgacc
     8041 tgaccgtcca gatgatttcg gaccgactgt ccaagcccga tgcaaccgcg ggcgtgatct
     8101 tcgacggctt cccacgaact cgacctcagg ccgaagcgct ggacaggatg ctcgacaaga
     8161 agggcgttga agtgaccgcg gcgctgtaca tcgacgttgg ccgcgaggag ctcgtgcggc
     8221 ggctctcggg tcgctggctg tgcaccgaat cgtccgacca cgtctaccac gaggtggcgc
     8281 ggccgccaca gaaggccggc gtctgcgact tcgacggtgc gccgctctac cagcgcgacg
     8341 acgacaagcc ggagacgatc gccgcccgtc tggagaagca gctgccgccg atgtacgagg
     8401 ttgtcgacca ctacgccggg accgacgtgc tgaataccgt tcggggcgac cacaccgtcg
     8461 atcaggtcac agacgatctg ctccacgcga tcgctctgcc gcactcaagc g
//
LOCUS       DATLCO010000154         2378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3934283, whole genome shotgun sequence.
ACCESSION   DATLCO010000154 DATLCO010000000
VERSION     DATLCO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3934283"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VMZ33_03870"
     CDS             complement(<1..983)
                     /locus_tag="VMZ33_03870"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HUR16397.1"
                     /translation="MPATVICGLQWGDEGKGKATDLLADRVSIVVRYQGGDNAGHTVV
                     IGDEVFKLHLVPSGALHPHITPVIGPGVVVNPQKLLEEMAMLRERGIGVDKVRVSHAA
                     HVIMPYHVALDGAREGTAATTGEQIGTTKRGIGPAYADRAARSGIRMGDLREPATIRR
                     RLEAALPEKNAILQHVYGAPTFEVDELVTLASAWGAALAPYITDTTALLQDALARGEL
                     VLLEGAQGTMLDLDHGTYPYVTSSNPISGGATTGGGIGPRQIDQVIGVLKAYATRVGS
                     GPMPTELDDEVGEHMVTVGREFGTTTGRRRRCGWLDLVPLRHAVRVNSVTSL"
     gene            1691..2179
                     /locus_tag="VMZ33_03875"
     CDS             1691..2179
                     /locus_tag="VMZ33_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16398.1"
                     /translation="MLRDAGHPDTNANGATDGHSNFAAHADANQAADRHTNRGTHGNA
                     DQDSAGDTDRYADADKGSDRDANQDSDADAHRDADQDSDADTHRHASPNSNRDVVANP
                     DLLADSHAYAHAELNTNSHYDTDSPAHRDANSRADRRSDHQPDPDGGTDPDALAKSDS
                     TT"
BASE COUNT          389 a          914 c          715 g          360 t
ORIGIN      
        1 aggctggtca cgctgttcac ccgcaccgcg tggcggagcg gcacgaggtc aagccagccg
       61 cagcgtcgtc gtcggccggt cgtcgtcccg aactcgcgac ccaccgtgac catgtgctcg
      121 cccacctcgt cgtcgagctc cgtgggcatc ggaccggagc caacccgcgt ggcataggcc
      181 ttcagcacgc cgatcacctg gtcgatctgg cgcggcccga tgcccccgcc cgtcgtcgct
      241 ccacccgaga tcgggttcga cgacgtcacg tatgggtacg tgccgtggtc caggtcgagc
      301 atcgtgccct gcgcgccctc cagcaacacg agctcgccac gggccagcgc atcctgaagg
      361 agcgcggtgg tatcggtgat gtacggcgcc aaggccgcac cccaggcgga cgccagcgtg
      421 accagttcgt cgacttcgaa cgtgggcgcg ccgtagacgt gctggaggat cgcgttcttc
      481 tcgggcaggg ccgcctcgag tcggcgacgg atcgtggccg gctcccgaag gtcgcccatc
      541 cggatcccgc ttcgtgccgc acggtcggcg taggccggtc cgatgccgcg cttcgtcgtc
      601 ccgatctgct cgccggtcgt tgccgcggtc ccctcgcggg ccccgtccag ggcgacgtgg
      661 tacggcatga tcacgtgcgc cgcgtgactg acgcgcacct tgtcgacgcc aatgccgcgc
      721 tcgcgcagca tggccatctc ctcgaggagc ttctgcggat tgaccacgac gcccgggccg
      781 atgaccggcg tgatgtgggg gtgcagcgcg ccgctcggca cgagatggag cttgaatacc
      841 tcgtcgccga tgacgacggt gtgtcccgcg ttgtcgccgc cctggtagcg cacgacgatc
      901 gagacgcgat cggccagcag gtccgtggcc ttgcccttgc cctcgtcgcc ccactgaagg
      961 ccgcagataa ccgtcgcggg catcagcgaa cgccgttcgt ggagggttgg gttctggata
     1021 gacgtgtgcc cgagccggct ccctcacggt gcgggagccc atcttcattg cgtgccggcc
     1081 tcacgggact ggcgacgctg tcgggcaaca gcttctcccc ttgtcgaacg cgggatctca
     1141 cggggtcccg cgcgccgaag tcggcagtct agcagcgggc tcaggcgacg atggctactg
     1201 cgcgcgcctc ggccgaatgc gtattcactt tgcaaggtgg ctcccctaga gttcgcgccg
     1261 ccgtgacaag cgccgtcgaa atcccgcgca tcctcgccgc gtgcctcgtg gcgatcgtcg
     1321 ctgcgctgtc cacgtggagc atcgccacac ctgacgtctc agcgattcat ttcgccccgt
     1381 gtacggccta ttcagtgccc acgtcgctat tcccgccatc cgtgaccggg acctaccacg
     1441 ccgacaacgg ccagggtttc gacgtcaccg tcgcattcga gcgcggaccg gctgccacag
     1501 ctcctggtcc ggtacaccgc gtggcgttcc gaggcgcctc catcaacgtt gcccacttct
     1561 cgtggttcac cgccacgcgc gggagcgctg gcacgcaggc ttacaacacg cccggcgtca
     1621 agacggacct cgcatggcgc tcagtcatgg gcgccactgg cgctccgttg ccgatcacat
     1681 cggtccggtt ttgctacgtg acgccggcca ccccgacacc aacgccaacg gtgccaccga
     1741 cggccactcc aactttgccg cccacgccga cgccaaccaa gccgccgacc gccacaccaa
     1801 cagaggcacc cacggcaacg ccgaccaaga ctccgccggc gacacggacc gctacgccga
     1861 cgccgacaaa ggctccgacc gcgacgccaa ccaggactcc gacgccgacg cccaccgcga
     1921 cgccgaccaa gactccgacg ccgacaccca ccgccacgcc agcccaaatt ccaaccgcga
     1981 cgttgtcgcc aaccccgacc tccttgccga cagtcacgcc tacgcccacg ccgagctcaa
     2041 caccaacagc cactacgaca ccgacagccc cgcccaccgc gacgccaaca gccgagccga
     2101 ccgacgctcc gaccaccagc ccgaccccga cggaggcacc gaccccgacg ccctcgccaa
     2161 gtccgactcc acgacctgag ccaacgccgg taaccccggc gggaggatca ggcggaaatt
     2221 ccttccccgg ctcgattccg cccatcacag ccgtcgggtc ggacgcgcct gccattggcc
     2281 agagcctgct cctggcgctg gtgttcctgt tgctcgccgc cttcccgggt cagctcctca
     2341 acaagacaat cgaggagaac tacacagaga tctcgggt
//
LOCUS       DATLCO010000155         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9147646, whole genome shotgun sequence.
ACCESSION   DATLCO010000155 DATLCO010000000
VERSION     DATLCO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9147646"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VMZ33_03880"
     CDS             complement(<1..626)
                     /locus_tag="VMZ33_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16399.1"
                     /translation="MGTARSSPIRVLLVDDHKLLTDALAGLLGHTDDIEVVGVASTVA
                     EAKSAALEPLDVALMDYLLPDGTGAEATREIKRHWPDAKVVILTAVADDETMLDAVEA
                     GADGYLTKDRAGVEVVQAVRDAHAGEMLLPRAVAYELAQRVGATRKVPTDPVHVHDLT
                     PRELEVLRMLVAGRSSRDICGELYIAPNTLRTHVQNVLAKLRVHSKLEA"
     gene            660..1253
                     /locus_tag="VMZ33_03885"
     CDS             660..1253
                     /locus_tag="VMZ33_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUR16400.1"
                     /translation="MTANTELVLGITRAFVPNGLKWRGVLRADLRPYLEALRRNGEFR
                     PRNEVESDPSWKQAIPYLLLRDGDRVFLMKRSKAGADQRLHDRFSIGVGGHVNPEDTD
                     VYGGLLREWEEEIDADFTPDFTPIGVLNDDQNSVGAVHLGLVFEADAGGRPVRIRETE
                     KLSGAFVTMPEVGAVAEKLETWSLLLYEFLLAEQSGG"
     gene            1261..>2351
                     /locus_tag="VMZ33_03890"
     CDS             1261..>2351
                     /locus_tag="VMZ33_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16401.1"
                     /translation="MDVRAVVRIFAGASLALLMAGQALGKGGDRVYIMPTTGVVDSIM
                     QSYIREGLNKAEREGAAAVLIQLNTPGGALDATREIVTSLLNAPLPVIVWVGPDGSRA
                     ASAGTFITMAAHVAVMSPNSTIGAATPISGDGSDIPDDLKAKVLEDTNALLRSIAQAR
                     ERSYDWALTTVVDARSYTGVEAVEQHGVDGTASTLQEAIDFAHGRTVTVGGQPTTLDL
                     AGATTTDLSMNPLQALLHLLSDPNIAFILFTIGFYGLLFELQNPNWVTGILGGISIIL
                     AFIGFGSLPLNVGGLLLVALAAVLLILEVNVTSHGLLTVGALICFALGAGALYTEPGT
                     PTAPDVSVALPVIVTMTALTGIFGVLIAIA"
BASE COUNT          392 a          751 c          787 g          421 t
ORIGIN      
        1 gcttcgagct tggagtggac gcggagcttc gccagcacgt tctggacgtg cgtccgcagc
       61 gtgttcggcg cgatgtacag ctcgccgcag atgtcgcgtg acgagcgacc ggcgaccagc
      121 atccgcagca cttcgagttc gcgcggtgtc aggtcgtgga cgtgcaccgg atcagtgggg
      181 accttgcggg ttgcgccgac acgctgggcg agctcgtacg cgactgcacg ggggaggagc
      241 atctcgccgg cgtgtgcatc gcggacggct tggaccacct caacaccggc acggtccttc
      301 gtgaggtagc cgtcggcacc cgcctcaacc gcgtcgagca tcgtctcatc gtcagcgaca
      361 gcggtgagga tcaccacctt ggcgtctggc cagtggcgct tgatctcgcg cgtggcctct
      421 gcacccgtgc cgtcgggcag caggtagtcc atcagcgcca cgtcgagggg ctcgagagcc
      481 gctgatttgg cctcggccac ggtcgaggcg acgcctacga cctctatgtc gtcggtatgc
      541 cccagcagac cggcaagggc gtcagtgagc agcttgtgat cgtcgaccag caggacgcgg
      601 atcggcgagg agcgtgcagt tcccatagct tccctgctcc gaaggtagca tcggatgacg
      661 tgactgccaa taccgaacta gtccttggca ttacgcgcgc atttgtcccg aacggattga
      721 agtggcgcgg cgtgctgcgc gcagacctac gtccgtacct ggaagcgttg cgcaggaacg
      781 gcgagttccg tccacgcaac gaggttgaga gcgacccctc ctggaagcag gcgattccct
      841 acttgctcct gcgtgatggc gatcgcgtct ttctcatgaa gcgcagcaag gccggcgccg
      901 accaacgcct gcatgaccgc ttttcgattg gcgtcggcgg acacgtcaac ccagaggaca
      961 cagacgtata cggcggcctc ctgcgcgagt gggaggagga gatcgacgcg gatttcacgc
     1021 ccgacttcac gccgatcggg gttctcaacg atgaccagaa ctcggtcggc gccgttcacc
     1081 tcggccttgt cttcgaggcc gatgcaggtg gtcggccggt ccggattcgg gagacggaga
     1141 agctgtcggg cgcgttcgtc accatgcccg aagtcggagc cgtggcggag aagctcgaga
     1201 cctggagcct cctgctgtat gagttcttgc tcgcggagca atcgggcgga taatcagctc
     1261 gtggacgtga gggctgtggt ccgcatcttc gcgggggcga gccttgcgct gctcatggcg
     1321 ggccaggcac tcggcaaagg tggcgatcgc gtctacatca tgccgacgac gggcgtggtc
     1381 gattcgatca tgcagtccta cattcgcgaa ggcctgaaca aggccgagcg cgagggcgcg
     1441 gcagcagttc tgatccagct caacacgccc ggtggggcgc tggacgcgac gcgcgagatc
     1501 gtcacgtcgc tcctgaacgc gccgctgcca gtgatcgtat gggtcggccc agacgggagt
     1561 cgcgcagcca gtgccggcac gttcatcacg atggccgccc atgtggcggt gatgtcgccc
     1621 aactccacca ttggcgccgc gaccccgatc agcggcgacg gcagcgacat tcccgacgat
     1681 ctgaaggcca aggtcctgga ggacacgaat gcattgttgc gttcgatcgc ccaggcgcgc
     1741 gagcgcagtt acgactgggc actgacgact gtcgtggatg cgcgctcgta cacgggtgtc
     1801 gaggcggtgg agcagcacgg ggtcgacggg acggccagca cgctccagga agccatcgac
     1861 ttcgcccacg ggcggacggt gacggtgggc gggcaaccga ccacgcttga tctcgcaggc
     1921 gcgaccacga ccgacctctc aatgaacccg ctacaggcgc tgctgcatct cctgtccgac
     1981 ccgaacatcg ctttcatcct gttcacgatc gggttctacg gactgctctt cgagctccag
     2041 aaccccaatt gggtcacggg gatcctgggc ggcatttcca tcatcctggc gttcatcggc
     2101 ttcggcagcc tgccgctcaa cgtgggcggg ctgctgctcg tcgcgctggc cgcggtcctg
     2161 ctgatcctgg aggtcaacgt aaccagccat ggcctgctga cggtgggcgc cctgatctgt
     2221 ttcgccctgg gcgctggggc cctgtacacg gagccgggca cgccgaccgc acctgacgta
     2281 tcggtggccc tgcccgtgat cgtgaccatg accgcgctga cgggcatctt cggcgtcctc
     2341 atcgccattg c
//
LOCUS       DATLCO010000156         4213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5427648, whole genome shotgun sequence.
ACCESSION   DATLCO010000156 DATLCO010000000
VERSION     DATLCO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5427648"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VMZ33_03895"
     CDS             <1..289
                     /locus_tag="VMZ33_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16402.1"
                     /translation="INVAEGEKQANILRAEGRRQSQILEAEGYSQALERIFNAAKAID
                     EKTMTLQYLEALKGLGAGPATKFVIPLEFTELAGRISNFAERGFSKAAAGE"
     gene            372..1955
                     /locus_tag="VMZ33_03900"
     CDS             372..1955
                     /locus_tag="VMZ33_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HUR16403.1"
                     /translation="MATLVTAVVNGLRGELIRVEIDVAPGLPACHIVGLPDAALSEAR
                     ERVRGAIRNSGFDYPASRITVNLAPADRRKRGAAYDLAIAVGILVASGQIRRGGGTWA
                     LLGELSLSGTVEPVTGVLPMVTTLARLGYRRVIVPMANVGEARLSIPKVEVVGVVGLD
                     DAARLVAGPRGLRALAAERKPNLEVSGRSESTLRGTKAASEPLTGVLEPVDLADIRGQ
                     RHARWAFEVALAGGHNLLLVGPPGAGKTLMARSIPGLLPPLTDEEALEVTVIESVAGL
                     GDGTLRRQRPFRAPHHTASYAALVGGGPALTPGEATLAHHGVLFLDELAEFDRPTLDA
                     LRQPLEEGMLTIARVHGHIRYPARFQLIAAMNPCRCGYADDPDRQCKCPLGDPERYVR
                     RVSGPLLDRIDMQIEMARVPPKELLGATSAEASSVVKERIVAARDIALARNGGVANAR
                     LNARTLSAVANVTKSANNSLNELAAKDHLSARSLHRIMRVARTIADLGGRADVAQEDV
                     LAAGAMRDPGRGKDERLAA"
     gene            1952..3115
                     /locus_tag="VMZ33_03905"
     CDS             1952..3115
                     /locus_tag="VMZ33_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HUR16404.1"
                     /translation="MSDTDADRISEREAWIVLSSVDGIGEILMPLLVSTFRGARNVLA
                     ATRDGGVAAFNEDWRRRNGRPALNTPAFAELVAAAHDPAKLLGKLDELGLWTYTSLDA
                     DYPPRLRDLDPPPAVIHGAGEPSHLCADKIVAIVGTRRPTPDGRDLAGRVARRLVEFG
                     AVVVSGLAVGIDGAAHAAVVEKDAPTIGVIGGGHLFPGPRAHRRLRGEVVAKGGAIIS
                     ERYPTVHPTIGTYPRRNRIIAALGDATIVIEAPRKSGALITAKAAMEIGRPVFVAPGR
                     IGDWSTVGSLNLLRESPARPLVGIDELIEDLGFLKAPAGQTEAVVPLLQSLSPTERAV
                     AERLKVAPAGLDALVADTGLPPGTISSAVTLLMLRGWAQSIGPAYAAAGPLAR"
     gene            3146..3511
                     /locus_tag="VMZ33_03910"
     CDS             3146..3511
                     /locus_tag="VMZ33_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16405.1"
                     /translation="MPRERPPGETARVITNVIVHLHNDLPIVVDMEGLPKGSDRSIRC
                     TNVRTVDGKRPTFVNDRHSTFVFPMSMIRLLEVPADGTHQGNTAIVTPEPQGPPPPPP
                     PPLEDDEPDDDLLARIRSV"
     gene            3561..>4213
                     /locus_tag="VMZ33_03915"
     CDS             3561..>4213
                     /locus_tag="VMZ33_03915"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HUR16406.1"
                     /translation="MTKNLVIVESPAKARTIERYLGPDYRVLASYGHVRDLPENPGKN
                     KLGVDVDHDFAPEYVVSEDRKKQVAIITREAKTADAVYLATDLDREGEAIAWHVAEAA
                     KVNPEKARRVTFSEITADAIREAFEHPRGIDRNLVDAQQTRRILDRLVGYTLSPLLWR
                     KVQSGLSAGRVQSVAVRLVVDREREIRGFTAREYWTIEALLATDKGETFAAELVRIDG
                     "
BASE COUNT          708 a         1407 c         1428 g          670 t
ORIGIN      
        1 catcaacgtc gccgagggcg agaagcaggc caacatcctg cgcgccgagg gtcgacgcca
       61 gtcgcagatc cttgaggctg agggttactc gcaggctctc gagcggatct tcaacgccgc
      121 caaggcgatc gacgagaaga ccatgaccct ccaatacctc gaggcgctca agggcctcgg
      181 cgctgggccg gccacaaagt ttgtgatccc gctcgaattc acggagctcg ccggacgtat
      241 cagcaacttc gccgagcggg gcttctccaa ggctgccgcc ggggagtgac ccgccgcggg
      301 cgatcggcgt aaggcgcgga taagccggcg ttgaccggca ggcgagtggc cgtggctacc
      361 gtcggggggt gttggccacc cttgtcactg ccgtcgtcaa cggtctgcgc ggcgagctca
      421 tccgcgtcga gatcgacgtc gcgccagggc tcccggcgtg ccacatcgtc gggctcccgg
      481 acgcagctct gtctgaggcg cgtgaacgtg tacgcggggc gatcagaaac tcgggcttcg
      541 actacccggc ctcccgtata accgtaaacc tggcgccagc cgatcggcgc aagcgcggcg
      601 ccgcctatga cctggccatt gcagtcggga tcctggtcgc gtcgggccag atccggcgtg
      661 gtggcggcac gtgggcgttg ctgggcgagc tgtcgttgtc cggcaccgtc gagccagtca
      721 cgggtgtgtt gccaatggtg acgacgcttg cccgactcgg ctacaggcga gtcatcgttc
      781 cgatggctaa cgtcggcgag gcccggctat ccattcccaa ggttgaggtg gtaggcgttg
      841 tcgggctcga cgacgccgcg cgtctggttg ccggtccgcg tggcctcagg gcattggctg
      901 ccgagcgcaa gccgaacctg gaggtgtccg gccgtagcga atcgacgtta cgtggcacga
      961 aggcggcgtc cgagccgctg acgggggtgt tggaacccgt cgatctcgcg gatatccgtg
     1021 ggcagcggca cgcgcggtgg gccttcgagg tagcacttgc cggcggtcac aacctgctgc
     1081 tcgtcgggcc acccggcgcg ggcaagacgc tcatggcgcg gtccatcccg gggctgctcc
     1141 cgccgctgac ggacgaagag gcgctcgagg tgacggtcat cgaaagcgtc gcagggctgg
     1201 gcgacggtac cctgcgccga cagcggccct ttcgggcgcc gcatcacact gcgtcgtacg
     1261 cggcactggt gggtggcggc ccagccctca cgccgggcga ggccaccctc gctcatcacg
     1321 gtgtgctgtt tctcgatgag ctggccgagt ttgaccggcc cacgctcgat gcgctccgcc
     1381 agcccctgga ggaggggatg ctgaccatcg cgcgtgtcca cgggcatatc cgctatccgg
     1441 cgcggttcca gctgattgcg gccatgaacc cgtgccgctg cggttacgcc gacgaccccg
     1501 atcggcagtg caagtgtccg cttggcgacc ccgaacgcta cgtcaggcgc gtcagcggtc
     1561 cgctgctcga ccgcatcgac atgcagatcg agatggcacg ggtcccgccg aaggagctcc
     1621 ttggtgcaac ctcagccgaa gcaagctccg tggtcaagga acggatcgtg gcggcgcggg
     1681 acattgcctt ggcgcgcaac ggtggtgtgg ccaacgcgcg gctgaacgca cgaaccctta
     1741 gcgcggtggc caacgtgacc aaatcggcca acaactcact gaacgagctg gccgccaagg
     1801 atcacctcag cgcgcgatca ctgcatcgga tcatgcgcgt ggcccgaacg atcgcagacc
     1861 tcggtggccg agcagacgtc gcgcaggagg acgtgttggc cgcaggcgcg atgcgcgacc
     1921 caggacgggg caaggacgaa aggctcgcag cgtgagcgac acagacgccg atcgcatcag
     1981 cgaacgcgaa gcgtggatcg tactttcatc tgtcgatgga atcggcgaga tcctcatgcc
     2041 cctgctcgtg tccacgttcc gcggcgcccg gaacgtgtta gccgccaccc gagacggtgg
     2101 agtggccgcg tttaacgagg actggcgtcg gcgcaacggc cgcccggcac tgaatacgcc
     2161 ggcgtttgct gagctggtcg cggccgctca cgacccagcg aaacttttgg ggaagctgga
     2221 cgagctgggc ctgtggacgt acacgtcgct cgacgccgat tacccgccgc gtctgcgcga
     2281 tctcgatcca ccgccggccg tgatccacgg cgccggtgag ccgtcacatc tctgcgcgga
     2341 caagatcgtc gcgatcgttg gcacccggcg gccgacaccg gacgggcggg acctggccgg
     2401 gcgcgtggca cgtcgccttg tggagttcgg cgccgtcgtc gtgtccggtc tggcagtcgg
     2461 catcgatggc gccgctcacg ctgccgttgt ggagaaggat gcgccgacga ttggcgtgat
     2521 cggtggcggg catctcttcc ccgggccacg cgctcatcgg cggctgcgtg gggaggtcgt
     2581 agccaagggc ggagcgatca tcagtgagcg atatccgacg gtgcatccga cgatcggcac
     2641 ctatccgagg cggaaccgga tcatcgccgc gctcggcgac gcgacgatag tcatcgaagc
     2701 gccgcgcaaa agtggtgcgc tgatcacagc caaagccgcg atggagatcg gccgtccggt
     2761 gttcgtggcg ccgggacgca tcggcgattg gtcgaccgtc gggtcgctaa accttctccg
     2821 cgagtctccg gcacggccgt tggtagggat cgacgagctg atcgaggacc tcggtttcct
     2881 gaaggcgccg gctggccaga ccgaagctgt cgtgcccttg ctccagtcgc tcagcccgac
     2941 tgagcgcgcc gttgccgagc gcctcaaggt tgcgccggcc ggcctggatg cattggtcgc
     3001 agacaccggg ctcccgccgg gcacgatctc cagcgccgtg acgctgttga tgttgcgtgg
     3061 ctgggcgcag tcgatcggcc ccgcctacgc cgccgctggc cctctcgccc gatgacggac
     3121 cactccggac cggacatcgg taccgttgcc gcgcgaacgc ccacccggcg aaactgcccg
     3181 cgtgatcacc aacgtcatcg tgcatctgca caacgacctg ccgatcgtcg tcgacatgga
     3241 aggcctgccg aagggctcgg atcggagcat tcgctgtacg aacgtgcgta ccgtggacgg
     3301 caagagaccg accttcgtga acgaccggca ctcgaccttt gtctttccga tgtcgatgat
     3361 ccgccttctc gaggtgccag ccgacggcac ccaccagggc aacaccgcca tcgtcacgcc
     3421 cgaaccccag ggcccgccac cgccgccacc tccgccgctc gaggacgacg agccggacga
     3481 cgacctgctg gcacgcatca gatcggtctg aaacaaggcc gcgccgtcgc agggttgcgc
     3541 tccgagtaga ctcggaagac atgacaaaga acctggtgat cgtcgaatca ccggcaaagg
     3601 cgcgcacgat cgagcgatac cttggtccgg actaccgggt cctggcgtcg tacggccatg
     3661 tccgcgatct gccggagaac cccggcaaga acaagctcgg cgtggacgtc gaccacgact
     3721 tcgcgcccga atacgtggtg tccgaagacc gcaagaagca ggtcgcgatc atcaccaggg
     3781 aggcgaagac ggccgacgcc gtctaccttg ccaccgacct tgatcgcgaa ggcgaggcca
     3841 tcgcctggca cgttgcggaa gccgccaaag tcaaccccga gaaggcacgc cgcgtgacct
     3901 tctccgagat cacggctgac gccatccgcg aggccttcga gcacccgcgc ggtatcgacc
     3961 gcaacctcgt tgacgcgcag cagacgcggc gcatcctcga tcgcctcgtc ggctacaccc
     4021 tgtcgcccct gctctggcgg aaggtccagt cgggcctgtc ggctggccga gtccaatcgg
     4081 tcgccgtgcg cctggtcgtc gaccgtgagc gcgagatccg cggctttacc gcgcgtgagt
     4141 actggacgat cgaggcgctg ctggcgacgg acaagggtga gacattcgcc gcggagctcg
     4201 tccgtatcga cgg
//
LOCUS       DATLCO010000157         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2449111, whole genome shotgun sequence.
ACCESSION   DATLCO010000157 DATLCO010000000
VERSION     DATLCO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449111"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VMZ33_03920"
     CDS             complement(<1..507)
                     /locus_tag="VMZ33_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16407.1"
                     /translation="MTAPPIVCERVSYEYPSGVRALAGVDLRLDGGEAVAIVGQNGSG
                     KTTLVRHFNGLLKPTQGRVHVGPAETRTTRIAQLASMVGLAFQDPDRQIFAGTVDGEV
                     SFGARNVGLKGDDLEGAVVSALDSVGLASERKTNPYDLGYSRRKLLTIASVLAMRTPI
                     IVLDEPTTG"
     gene            complement(504..1298)
                     /locus_tag="VMZ33_03925"
     CDS             complement(504..1298)
                     /locus_tag="VMZ33_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16408.1"
                     /translation="MLTLRNISYRYAGTNKAVLSGVNLSIADGEAVGLVGASEGGKTT
                     LCLVASGLAPRSIGGILEGALLVDDRDVRELPMHEMAPMVGICFQNPHTQLSQVAETV
                     FEEIAFGSMNLGLDRLDVAARTTDAMEALGIIDLAERDPRQLSGGQMQLVAVAGLLAM
                     RPGHLVLDEPTAQLDPEGTHLVGEALAGIVSRGTGLLIAEHDTDLLASLCSRVVALDA
                     GAVVMDGPATDVLGDKRLADLGVVPPSRVRLSRSLETAGVLDVALP"
     gene            complement(1311..>2172)
                     /locus_tag="VMZ33_03930"
     CDS             complement(1311..>2172)
                     /locus_tag="VMZ33_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HUR16409.1"
                     /translation="DPRRAGHAHQGALPAGREARRARARLLTVPEFLGRPGPSPYHRL
                     NPLTKLVIAVGTTAAAVVLGGVFWPLALVAAFVLAPAVLAGVLPKLLRFSLLFSLPIA
                     LSALVLNLFFFPGGQTVLLQLGPITATAEGLSFAIEILARIAAISGAVTLFYLTTKPS
                     ELVLDMERRGVPARLAFVANASVQTVPAMAERASAIVAAQRARGLDTEGSLWRRIRGV
                     LPIVGPVMLGSISEVEERTMTLEARAFTRPGRRSLLWSPPDSGWQRLLRYGLVLAFVA
                     VVVARAAGLV"
BASE COUNT          398 a          748 c          730 g          296 t
ORIGIN      
        1 gccggttgtc ggctcgtcga ggacgatgat cggcgtccgc atcgccagca ctgacgcgat
       61 cgtcagcagc ttgcggcgcg agtagccgag gtcatacggg ttggtcttgc gctcggacgc
      121 aaggccgacc gagtcaaggg cggacacgac cgcaccctcc agatcatcgc ccttcagccc
      181 gacgttgcgg gcgccaaacg agacctcgcc atcgacggtt cccgcgaaga tctggcggtc
      241 ggggtcctga aacgcgaggc ccaccattga cgccagctgg gcgatcctgg tcgtccgtgt
      301 ttcggccggg ccaacgtgca cgcggccctg cgttggcttg agaaggccat tgaagtggcg
      361 cacgagcgtc gtcttgccgg agccgttctg gccgactatc gccacggcct caccgccgtc
      421 gaggcgcagg tccacgccgg ccagggcgcg aacaccgctg gggtactcat acgagacccg
      481 ctcgcagacg atcggcgggg cggtcacggg agagctacgt ccaacacgcc agcagtctcg
      541 agggaacgac taaggcggac gcgcgacggc gggaccacgc cgagatccgc caaccgcttg
      601 tcacccagga catccgtggc tggtccatcc atgactacgg caccggcatc caacgccacc
      661 acccgtgagc agagcgacgc cagcaggtcc gtgtcgtgct ccgcgatgag caaccccgtc
      721 ccgcggctga cgatgcccgc caacgcttcg ccaaccaggt gcgtgccctc cgggtcgagc
      781 tgcgccgtcg gctcgtcgag gacgaggtgc cccggccgca tggcgagcag cccagccacg
      841 gccacgagct gcatctgacc gccggatagc tgacgtgggt cgcgctcggc cagatcaatg
      901 atgcccagag cctccatagc gtcggtcgta cgtgcggcga cgtcgagtcg atccaacccc
      961 aggttcatcg acccgaaagc gatctcctcg aacaccgtct cagcgacctg cgagagctgt
     1021 gtgtgcgggt tctggaagca gatgccgacc atcggcgcca tctcgtgcat cggaagctca
     1081 cggacgtctc ggtcgtcaac gagcaaggcg ccttcgagga ttccgccgat tgaccgtggc
     1141 gcaagtccgc tggcaacgag gcacagggtg gtcttgcccc cttcgctggc gccaaccagg
     1201 ccaaccgcct cgccgtcggc gatcgacagg ttgacgccgc tcagcacagc cttgttcgtg
     1261 ccggcgtagc ggtacgagat gttgcgcagc gtgagcatgg gtgcggcggc tcagaccagg
     1321 ccggccgcgc gcgcgaccac cacagccacg aacgccagga ccaggccgta ccggaggagg
     1381 cgctgccaac cgctgtcagg cggcgaccac agcagggatc ggcgacctgg ccgcgtaaac
     1441 gcgcgcgcct caagcgtcat cgtccgctcc tcgacttcgc tgatcgagcc cagcatgacc
     1501 ggcccgacga tcggcaagac gcctcggatg cgccgccaca aagatccctc cgtgtccagg
     1561 ccgcgtgccc gctgcgcggc cacgatcgcc gatgcccgct cggccatggc cggcacggtc
     1621 tggaccgaag cgttggccac gaaagccagc cgcgcaggca ccccccgtcg ttccatgtcg
     1681 aggacgagct ccgagggctt ggtcgtcagg tagaaaagcg tcaccgcgcc ggagatcgca
     1741 gcgatccgcg ccaggatctc aatggcaaac gacaggccct cggccgtcgc ggtgatcggc
     1801 ccgagctgca gcagaacggt ctggccgcca gggaagaaga acaggttaag cactagagca
     1861 ctcagcgcga ttggcaggct gaacagcaac gagaagcgca gaagcttggg caggacgccg
     1921 gccagaacgg ccggcgcgag gacaaaggca gcgacgaggg ccagcggcca gaacacgccg
     1981 ccgagtacga ccgcggctgc ggtcgtccca accgcgatga ccagcttcgt aagtgggttg
     2041 agccggtggt agggactcgg gccggggcga cccaggaact ccgggacggt cagaagccgg
     2101 gctctggcgc gacgagcttc tcgccctgcg ggaagcgcgc cttggtgcgc gtggccagcg
     2161 cgccgaggat ca
//
LOCUS       DATLCO010000158         9556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8304318, whole genome shotgun sequence.
ACCESSION   DATLCO010000158 DATLCO010000000
VERSION     DATLCO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9556
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8304318"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VMZ33_03935"
     CDS             complement(<1..438)
                     /locus_tag="VMZ33_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUR16410.1"
                     /translation="MNSSETGHVEVPGGRLYYEVEGDGPPVTLVHAGVANLRMWDGQV
                     AAWRDRYRVIRYDTRGFGQTETDDVPYSNRDDLAFVLDHVGVEQTHLLGLSRGSMIAT
                     DFAVENPQRVKSLVWVAGGLRGFEPPEDPRLQAMWPEMERLEEA"
     gene            complement(435..1607)
                     /locus_tag="VMZ33_03940"
     CDS             complement(435..1607)
                     /locus_tag="VMZ33_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HUR16411.1"
                     /translation="MNGDAADSEPLVVYGAVSDRYDFGPEHPLTPRRFGPSIDLMKTV
                     GATRFVEPRLATDEEIQRLHHPEYIEAVRRLSGDLHNSRPTAGIGPGDCPPFADMHEV
                     SASVAGGSIDAVDRILANEMLHAFHPGGGLHHAMAGRASGFCIYNDVALAVARARDAG
                     HRVMYVDLDVHHGDGTQALFWDDPAVLTLSIHETGMTLFPGSGFIDERGSEAARGTKV
                     NIPLQPYSGDASWIVAVERAIPELAEAFRPTFLVTQHGCDTHAYDPLAHLRLTTRAYR
                     VATLLLDAVAHEFCEGRWLATGGGGYDAYRVVPRSWSLVWLAQAHVEPPPETDASWRE
                     RWSASARQHDQAPPPEHFLDPLDFVKPDSAQIMGANAAAVDKALTGAITQMQEARP"
     gene            complement(1604..2164)
                     /locus_tag="VMZ33_03945"
     CDS             complement(1604..2164)
                     /locus_tag="VMZ33_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16412.1"
                     /translation="MIVVVGRPRLDDAGAPAGLATLVCLAARGHGGGAELVGRVSDNP
                     AGDQAIVELGRAGVGHAAVLRDPTGASGQIEAADVELALRYITECNVLVVAEPLADDA
                     LRAVIDGAAYHRAALVVITPSDAVTLSTQSLPPEATVLQAPDEGEGPFAQVVGRYAAR
                     LDAGDQPADAWRAAMESSGWEQAGQA"
     gene            complement(2161..3711)
                     /locus_tag="VMZ33_03950"
     CDS             complement(2161..3711)
                     /locus_tag="VMZ33_03950"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUR16413.1"
                     /translation="MTLEGAAEKGFQTDQDVEPTEQETESTTDVLTAMPDSAAAIAPV
                     TLTMPTSTTEDATDLSVLDNRPSPGEPRDYRFPRFERAVLANGLTVVSAHVPGRALLA
                     AQLLMPGGAWNEPFEQRGVSVLAARAMPEGTTERDANEFTEASERLGADIHADASWEA
                     LSATMEVPRSRLGPALALLAEMAFEPAFPGEEVDRLRDERLNDLLQAWSDPRRRAERV
                     FPETIYSTDTPYNRPLAGIQSTVGPLDREAVVARHRAATNPSAATLVIAGDLAGLDLV
                     RLAEDHLGTRSATGPSTAAAGPTDAAGHPAGARIVLVDRPGAPQSELRIGHIGVPRKH
                     PDFHAMAVLNAILGGTFNSRLNRVIREEKGYSYGIHSSFEMRRRAGPFAVRCAVETAV
                     TVPALTETLRIISEMTQRAPDDEELSIARDYLVGVFPLRFETAAQVASALGGLVIFGL
                     PDDELDRYRPAVAAVTADDVVGAAQRNIRPNDLSLVIVGDASQIEAPLRDAGLGDVTV
                     IPADATPE"
     gene            complement(3708..5057)
                     /locus_tag="VMZ33_03955"
     CDS             complement(3708..5057)
                     /locus_tag="VMZ33_03955"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUR16414.1"
                     /translation="MPDPQPMSSDLPPDLPTVRFSQRRLENGMRVIVAPDSLAPVVAI
                     NIWYVVGSRHEQPGRTGFAHLFEHFMFQGSRHVAKAEHFSIVQSAGGVNNATTYFDRT
                     NYFETMPSHQYEIGLWLEADRMATLLDALDQENLDNQREVVKNEKRQSYDNRPYGSFY
                     ERLMAAVFPADHPYHHTPIGSMEDLNAASVEDVVNFFSAWYAPNNAVLSIVGDVDEEA
                     AHAAAERFFGPIRANWSIEQPPLPAIEPTIGHEMREVVPDAVPLTRVHFGFRCPAYGT
                     KEFDALEVCSQILAGGRGSRLYRNLVREKKIAQDVTAFGLPLVAGASFFGGWVTVRPD
                     SDEETCEEAFLEQLDRLTEELVTDDELARARALIEASELGALSRVEEVADRLSMFAAL
                     FDRPELVNEQLPRYLAITAEDIRDAARQVFRADNRVVLTYVPRPGEADSANEDEEAA"
     gene            5256..6674
                     /locus_tag="VMZ33_03960"
     CDS             5256..6674
                     /locus_tag="VMZ33_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16415.1"
                     /translation="MAEMAASQPTESPAGSESTGRPTRALPMVHLLNISVYWLGINVI
                     WAGLGYAIFQARFTAMFGETLATGYAGLLETVPLIVAVLVQPTIAAISDYTVSRWGRR
                     KPYIVIGAVLDVVFLLGIAIANDFVSILAFVMLLQFSSNFAQGPFQGYVPDLVPEKQV
                     GTASGLMGLMIIAGQVVGVGIATLGMSQLGDIRPLYGTPEGSELARQAFFLPTLGLAV
                     IEFVTMIPLVLFVREGRAAPDRGGRTWRQIALGAWGTDILNERSYVWLLVSRLFFLAC
                     PTILTFMGFFYLVRTLGIPENETGGPLTIIAAIIGVTTAITTIPAAKLSDRIGRKKTI
                     YIAIGLGMCGMAGAAVAPSFPFLIAALLPVGVSAGAFLAVDWALMTDIIPKQTSGRYM
                     GISNVATGLSGPLGRISAGVLVTLLVLIGMPPELRGIDDAPASQSSFYALGPRIAIAT
                     SLVFFAISAWALRHVDERRRED"
     gene            6632..8095
                     /locus_tag="VMZ33_03965"
     CDS             6632..8095
                     /locus_tag="VMZ33_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16416.1"
                     /translation="MGPAPRRRATSRGLTLRPTYTRPIEATEPIIDPGAAAIREPVVH
                     APGRPTRDLPLSQLLTLSIFWLGINATWTGLHVIVLPKRMEALFGPASAGLGLGLITI
                     AGVITAIIVQPTIGAISDYASTRWGRRKPFIVIGSALDLVFLFAVASADTYLALVAAL
                     VLLQFSSNFAQGPFQGYVPDLVPARQVSLASGLMGVMIIFGGICGVGIASIGYFQLQA
                     GMSAEVVRATLFLPTIALGVIETSTMILLAVRVDEGRAAVPREGRPWRSIALSAWGTD
                     ILRQNSYIWLLVSRLCYLALPGVITAYAVFVLERSFGLAPQVAAPYLIVTGLIVALST
                     AAATLPAARLSDRYGRKRVIYGAYGLAAIGLVGLSLSPVLVLSLLALVPVGLSAGAFL
                     VVDWALMTDIIPKARAGRYMGISNVATASAGPVGLALAGVVLYVVTRAGLETPDAADI
                     TSTLYGLAPRAAIGSMLLLLAIAVLALTRVDETRRED"
     gene            complement(8112..9029)
                     /locus_tag="VMZ33_03970"
     CDS             complement(8112..9029)
                     /locus_tag="VMZ33_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16417.1"
                     /translation="MASYVVRRILWLIPILFAVSVITFSLMHLVPGGPWATEKQVAPG
                     VEAAIERKYHLGEPAWQQYGRWVIEMAQGDFGPSFKYGDRSINQLVTQGLGPTLHLGL
                     MAFVLGIGLGVPLGVIAALGHNRWPDYLATTISVLGIATPTFVLAILLVVFFSVGLKI
                     FPAFGWGGPETWVLPVIALGGFQVAQLARYTRASMLEVSHKEYIVTAHSKGLLPRTVV
                     MRHMVRNALIPVATVLGPMLAFLINGSFIIEAFFGVPGIGKYYVQGIAQRDYGLIMAM
                     TVIYAVTIAVLNTVVDILYGYIDPRIRYR"
     gene            complement(9050..>9556)
                     /locus_tag="VMZ33_03975"
     CDS             complement(9050..>9556)
                     /locus_tag="VMZ33_03975"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR16418.1"
                     /translation="PALVLLVGPSGAGKSTFASRNFSGTEILSSDRARELLSDNPSDQ
                     GASTEAFRVVGLIANGRLKRRLMTVIDATNLRAASRSGYRRLAARYGIPTVTIAFDLP
                     TDQYALHNRNRPDRVVDDYVITDQAERMRDALLVLPDERYAALYVLRSPEEIAAAVIE
                     RAASRGRA"
BASE COUNT         1506 a         3182 c         3226 g         1642 t
ORIGIN      
        1 ggcctcctcg aggcgctcca tctcaggcca catcgcctgc aggcgagggt cctccggcgg
       61 ctcgaagcca cgcagcccgc ccgcaaccca gacgagtgac ttgacgcgct gcgggttctc
      121 gacggcgaag tcggtggcga tcatcgaacc acgagacagc cccagaagat gggtttgctc
      181 cacgccgacg tggtcgagaa cgaacgcaag gtcgtcgcga ttcgaatacg gcacgtcgtc
      241 ggtctcggtt tggccgaagc cccgcgtgtc gtagcggatc acccggtatc gatcccgcca
      301 ggcggcgacc tgtccgtccc acattcggag gttggccacg ccagcatgga caagggtgac
      361 cggcgggccg tcgccctcaa cctcgtagta gaggcggcca cccggtactt cgacgtgacc
      421 ggtctccgat gagttcatgg gcgcgcctcc tgcatttgag tgatggcgcc cgttagcgcc
      481 ttgtcgacgg cggccgcatt ggcgcccatg atctgggcag agtctggctt gacgaagtcg
      541 agcgggtcca ggaaatgttc cggcggcggc gcctggtcgt gttgacgcgc tgaagcggac
      601 caacgctccc gccacgaggc gtcggtctcc gggggtggtt cgacgtgcgc ctgggcgagc
      661 cacaccagcg accatgaacg gggcacgacc cgataggcgt cgtagccgcc gccgcccgtg
      721 gccagccacc gaccctcgca gaactcgtgg gcaacggcgt cgaggagcag cgtcgcgacg
      781 cgataggcac gcgtcgtcag gcgcaggtga gcgagcgggt cgtaggcgtg cgtgtcgcaa
      841 ccatgttgcg tgacaaggaa ggtgggacgg aacgcctcgg ccagctccgg gattgcccgt
      901 tcgacggcga cgatccatga tgcatcgccg ctgtacggct ggagcgggat gttgactttc
      961 gtcccgcgag cggcttcgct gccacgctcg tcgatgaagc cagatcctgg aaacagcgtc
     1021 atgcctgtct cgtggataga cagcgtgagc accgctggat cgtcccagaa aagagcctgc
     1081 gtgccgtcgc cgtggtgaac atcgagatcg acatacatca ccctatggcc ggcgtcacgg
     1141 gctcgcgcaa cggcgagggc cacatcgttg tagatgcaga accccgacgc gcggccggcc
     1201 attgcatggt ggaggccgcc gccgggatga aatgcatgga gcatctcgtt ggcaagaatg
     1261 cggtcgaccg catcgatcga cccgccggcc acggacgccg agacctcgtg catgtcggcg
     1321 aatggcggac agtcgccggg gccgataccg gcggtgggtc tcgagttgtg aaggtcgccc
     1381 gagaggcgac ggacggcctc gatgtactcc gggtggtgca ggcgctggat ttcctcatcg
     1441 gtggcgagtc gcggctcaac gaatcgggtc gcccccacgg tcttcatcag gtcgatcgag
     1501 ggcccgaacc ggcgcggcgt gagcggatgc tctggcccga agtcgtagcg atcgctgacg
     1561 gcgccgtaaa cgaccaacgg ctcggaatcc gcggcgtcgc cgttcacgcc tgccccgcct
     1621 gctcccagcc actgctctcc atcgcggcac gccacgcgtc agccggctgg tcgccggcgt
     1681 cgagccgcgc ggcatagcgg ccgacgacct gcgcgaatgg gccctcgccc tcgtcgggcg
     1741 cctgcaggac tgtcgcttcg ggtggcagcg actgcgtcga cagtgtgact gcgtcagatg
     1801 gcgtgatgac taccagcgcc gcacggtggt aggccgcccc gtcgatgacg gctcgcaacg
     1861 cgtcatcggc gagtggctcg gcgacgacga ggacgttgca ctccgtgatg tagcgcagcg
     1921 cgagctcgac gtcggcagcc tcgatctggc cgcttgcgcc cgttgggtcg cgaagcactg
     1981 cggcgtggcc gacgcctgcc cggcccagct caacgatcgc ctgatcaccg gctgggttgt
     2041 cgctcacccg gccgacgagc tcggcgccac cgccgtggcc gcgggccgcc agacagacga
     2101 gcgtggcgag gccggccggg gctccagcat cgtccagccg tggtcggccg acgacgacaa
     2161 tcactcgggc gtggcgtccg ctgggatgac cgtgacgtcg cccaaaccgg cgtcgcgcag
     2221 cggcgcctcg atctggctcg cgtcgccgac gatcaccagg gagaggtcgt tcggccggat
     2281 gttgcgttgc gcggccccga ccacgtcgtc cgctgtgacg gcagccacgg cagggcggta
     2341 acgatccagc tcgtcgtcgg gaagcccgaa gatgacaagg ccacccagcg ccgatgccac
     2401 ctgcgcagcg gtctcgaagc gcagcgggaa caccccgacc aggtagtcgc gggcgatgct
     2461 cagctcctcg tcgtcgggcg cgcgctgggt catctcggag atgatccgca gcgtttccgt
     2521 caaggcgggg acagtcaccg cggtctcaac tgcgcagcgc acggcaaatg gacctgcccg
     2581 tcggcgcatc tcgaacgacg aatggatgcc gtacgagtag cccttctcct cgcggatgac
     2641 ccggttgagg cgcgagttga acgtgccgcc gaggatggcg ttgaggaccg ccatcgcgtg
     2701 gaagtcgggg tgcttgcggg ggacgccgat gtgcccgata cgcagctccg actgcggcgc
     2761 gcccggacgg tcgacgagca cgatgcgggc gccggccgga tgtccggcgg cgtccgtcgg
     2821 acccgccgca gcggttgacg gtcctgttgc agagcgcgtg cccaggtgat cctccgcgag
     2881 ccgcaccaga tccagcccgg ccaggtcgcc ggcgattacg agggttgccg ccgacgggtt
     2941 cgtggctgcg cgatggcgcg ccacgacggc ctcacggtcg agcggcccta ccgtcgactg
     3001 gatgccggcg agcggtcggt tgtacggcgt gtccgtcgag tagatggtct ccgggaagac
     3061 gcgctcggca cgccgtcgcg gatcagacca cgcctggagc aggtcgttca gccgctcgtc
     3121 ccgaagccgg tcgacttcct cgcccggaaa cgctggctcg aacgccattt ccgcgagcag
     3181 cgccagcgcc gggccgagac gggagcgcgg cacctccatc gtggcgctca gcgcctccca
     3241 cgaggcatcg gcgtgtatgt cggcccccag gcgctcggaa gcctcggtga actcgttggc
     3301 atcccgttcc gtcgtgccct cgggcatcgc ccgcgcggcg agcactgaca ctccccgctg
     3361 ttcgaatggc tcgttccacg cgccgccagg catcagcagc tgcgcggcga gcagggcacg
     3421 ccccggtacg tgcgccgaaa ccacggtcag cccgttcgcc agcaccgccc gttcgaaccg
     3481 cgggaaccga tagtcgcgtg gctcccccgg tgacggcctg ttgtccagca ccgacaggtc
     3541 agttgcgtcc tcggtcgtcg aggtcggcat ggtcagcgtg accggcgcga ttgcggccgc
     3601 ggagtccggc atggccgtga gtacgtcggt ggttgactcc gtctcctgct cggtcggctc
     3661 tacgtcctga tcggtctgga agcccttttc ggctgcgcct tcgagcgtca tgcggcttct
     3721 tcgtcctcgt tggccgagtc ggcctcacct ggtcgcggta cgtacgtcag gaccacacgg
     3781 ttgtcggcac gaaagacctg gcgcgcggca tcgcgaatgt cctcggccgt gatggcgaga
     3841 tagcggggca gctgctcgtt gaccagctcg ggtcgatcga agagcgcggc aaacatcgag
     3901 agtcggtccg cgacctcctc cacgcgcgac agcgcgccca gctcgctggc ttcgatcagg
     3961 gcccgcgccc gtgccagctc gtcgtcggtc accagctcct cggtcaggcg gtcgagctgc
     4021 tcgaggaatg cctcctcgca cgtctcctcg tcactgtccg gacggactgt gacccagcca
     4081 ccaaagaatg aagcgccggc gaccagtggc agaccaaaag cggtcacatc ctgcgcgatc
     4141 ttcttttccc gaaccaggtt gcggtaaagg cgcgagccgc gaccaccggc gagaatctgc
     4201 gagcacacct ccagcgcgtc gaattccttc gtgccgtagg ccgggcaccg aaacccgaaa
     4261 tgaacgcggg tcagtggcac cgcgtcgggg acgacctcgc gcatctcgtg gccaatggtc
     4321 ggctcaatcg ccggcagcgg cggctgctcg atcgaccagt tggcgcggat aggcccgaag
     4381 aagcgttccg cggcagcgtg ggcggcctcc tcgtcaacgt cgccgacgat cgacagcacc
     4441 gcgttgttcg gtgcatacca ggcgctgaag aagttgacga cgtcctcaac gctggccgcg
     4501 ttgaggtcct ccatcgagcc gattggggtg tggtgatacg gatgatcggc cgggaacacg
     4561 gcggccataa ggcgttcata gaaggagccg tagggcctgt tgtcgtagga ctggcgcttc
     4621 tcgttcttga ccacctcgcg ctggttatcg aggttctcct ggtcgagggc gtcgaggagc
     4681 gtcgccatcc ggtcggcctc cagccacagg ccgatctcgt actgatgcga cggcatcgtc
     4741 tcgaagtagt tggtccggtc gaagtaggtg gtggcgttgt tcaccccacc cgcggattgc
     4801 acgatcgaga agtgctcggc ctttgcgacg tgacgcgacc cctggaacat gaagtgctcg
     4861 aacaggtgcg cgaagccggt gcgacccggt tgctcgtggc gtgagccgac cacgtaccag
     4921 atgttgatcg cgacgaccgg cgccaaactg tcgggcgcga cgatgacgcg cattccgttt
     4981 tcgagccggc gctggctgaa tcggacagtc ggcagatcgg ggggcaggtc actgctcatg
     5041 ggctgagggt cgggcatcga ggcggattgt cgcgcatcag tgggaggagg cgggatcgcc
     5101 gctatccggg tcacgcgtgc cgatcgcggg tgaccgtctg tgatcgttct gggcagggtt
     5161 cgagatcggt ccggtgccct gcgggaacca cagcggtcgg acttgcctcg tagcgcacgg
     5221 aggaacatag tccggcgcga gggaggcgcc gcgatatggc cgagatggcc gcaagccagc
     5281 cgactgagtc accagccggt agcgagagca cggggcggcc cacgcgcgcc ctgcccatgg
     5341 tgcacctgct caacatctcg gtgtactggc tcggcatcaa cgtcatctgg gcgggcctcg
     5401 gctacgcgat tttccaggcg cgtttcacgg cgatgttcgg cgaaaccctc gctaccggct
     5461 acgccggtct gctggaaacg gtgccgctca tcgtcgccgt ccttgtccag cccacgatcg
     5521 cagcgatatc ggactacacg gtcagtcgat ggggtcggcg taagccgtac atagtcatcg
     5581 gcgccgtcct cgacgtcgtc ttccttctcg gcatcgccat cgccaacgac ttcgtgtcga
     5641 tcctggcctt cgtgatgctc ctgcagttct cttcgaactt cgcgcagggg ccgttccagg
     5701 gctatgtgcc cgatcttgtg cctgagaagc aggtcggcac ggcaagcgga ctgatggggc
     5761 tgatgatcat cgccggtcag gtggtgggtg ttggcatagc cacgctcggc atgtcgcagc
     5821 tcggcgacat ccggccgctc tacggcactc ctgaggggtc cgaattggca cgccaggcgt
     5881 ttttcctgcc gacgctcggc ctcgcagtga tcgagttcgt cacgatgatt ccgctcgtgc
     5941 tcttcgtacg tgaagggcga gcggcccccg accgcggagg gcgcacctgg cgccagatcg
     6001 cactcggcgc ctggggcacg gacatcctca acgaacgcag ctacgtttgg ttgctcgttt
     6061 cgcgcctctt cttcctggcc tgcccgacga tcctcacgtt catgggcttc ttctacctcg
     6121 ttcgaaccct gggcattcca gagaacgaga ccggcgggcc gctgaccatc attgccgcca
     6181 tcatcggcgt gacgacggcg attacaacga ttccggcggc gaagctctcg gaccggatcg
     6241 gacgcaagaa gacgatctac atcgcgatcg ggctgggcat gtgcgggatg gccggcgccg
     6301 ccgtcgcgcc cagcttcccg ttcctgattg ccgccctgct gccggtcggc gtctccgccg
     6361 gcgccttcct cgccgtcgat tgggcgctga tgaccgacat catccccaag cagacgagcg
     6421 gcagatacat gggcatctcg aatgtcgcga cgggcctatc cggcccactt ggccgcatat
     6481 cggctggagt gctggtcacg ctgctcgttc tgatcgggat gccgccggag ctgcgcggta
     6541 tcgacgacgc gccggccagc cagagctcgt tctacgcgct cgggccccga atcgcgatcg
     6601 ccacgtccct ggtcttcttc gcgatctcgg catgggccct gcgccacgtc gacgagcgac
     6661 gtcgcgagga ctgactctcc ggcccactta cactcggccg atcgaagcga ccgaaccgat
     6721 catcgacccc ggggcggccg caatacgcga gcccgtcgtc cacgctccgg gccgaccgac
     6781 acgcgacctg cctctctcgc agctgctgac gctgtcgatc ttctggctgg gcatcaacgc
     6841 cacgtggacc ggcctgcacg tcatcgtgtt gcccaaacgg atggaggcac tgttcggccc
     6901 agcctcggcg ggtctgggcc tgggcctaat cacgatcgcc ggcgtgatca ccgcgatcat
     6961 cgtccaaccg acgatcggcg ccatctccga ctacgcctcg acgcgctggg ggcgacgaaa
     7021 gccgttcatc gtgatcggta gcgctctcga cctcgtcttc ctctttgccg tggcatcggc
     7081 cgacacctac ctcgccctag tcgccgcgct cgtgctcctc cagttttcgt cgaacttcgc
     7141 gcagggtccg ttccagggat acgtgcccga cctcgtgccg gcgcgccagg tgagcctggc
     7201 cagcggcctg atgggcgtga tgatcatctt cggcgggatc tgtggcgtcg ggatcgcctc
     7261 cattggttat ttccagctcc aagccggcat gtccgccgaa gtggtccgcg cgaccctgtt
     7321 cctcccgacg atcgcgctcg gcgtaattga gacatcaacc atgatcctgc ttgcggtgcg
     7381 cgtcgacgag gggcgcgccg ccgtgccgcg cgagggccga ccctggcgga gcattgccct
     7441 ttccgcgtgg ggcaccgaca tcctgcgcca gaacagctac atctggctgc tcgtctcgcg
     7501 cctgtgctac ctggcactcc ccggggtcat caccgcgtac gccgtattcg tgctggagcg
     7561 gtccttcggc ctcgcgccac aagtagcggc gccgtacctg atcgtgaccg gcctgatcgt
     7621 ggcgctgtcg acggccgcgg ccactctgcc agccgcccgt ctatcggacc gctacgggcg
     7681 caagcgcgtc atctacggcg cgtacggcct ggcggcgatc ggtctcgtcg gcctctcgct
     7741 gtccccggtg ctggttctca gcctgttggc gcttgtgccc gtgggcctgt cggctggtgc
     7801 ctttctggtg gtcgactggg ccctgatgac ggacatcatc cccaaggcac gcgccggtcg
     7861 gtacatgggc atctccaacg tggcgacggc ctctgccggt cccgtcgggc tcgccctggc
     7921 cggcgttgtc ctctacgtcg tcacgcgtgc cgggctggag acgcccgacg cggccgacat
     7981 cacgagcacc ctctacggtc tcgcaccgcg cgcggcgatt ggctcgatgc tcctgctgct
     8041 ggcaatcgcc gtgcttgcac tgacgcgcgt cgacgaaacg cgtcgcgagg actgaccgag
     8101 ctggcgttcg gtcagcggta ccggatccgc gggtcgatgt agccgtagag gatgtcgacc
     8161 acggtgttca ggaccgcgat ggtgaccgcg tagatcaccg tcatcgccat gatcagcccg
     8221 tagtcgcgct gggcgatgcc ctgcacgtag tacttgccga tccccgggac gccgaagaag
     8281 gcttcgatga tgaacgagcc gttgatcagg aatgcgagca tcgggcccag gacggtcgcg
     8341 accgggatga gcgcattgcg aaccatgtgg cgcatcacca cggtgcgcgg cagcaggcct
     8401 ttgctgtgcg ctgtgacgat gtattccttg tgcgacacct cgagcatcga agcccgcgtg
     8461 tatcgggcca gctgggctac ctggaagccg ccgagcgcga tgacgggcag aacccaggtc
     8521 tctggcccgc cccaaccgaa cgccggaaag atcttcaggc cgacgctgaa gaagacgacc
     8581 agcaggatgg cgagcacaaa ggtgggggtg gcgatcccga gcaccgagat cgtggtggcg
     8641 aggtagtccg gccagcggtt gtggccgagc gccgcgatga cccccagcgg caccccgagc
     8701 ccgataccga gcacgaaggc catcaagccg agatgcagcg tcggtccgag accttgcgtg
     8761 acgagctggt tgatgctccg atcgccgtac ttgaacgacg gcccgaagtc accctgagcc
     8821 atctctatga cccatcggcc gtactgctgc catgccggct cgcccaggtg gtacttgcgc
     8881 tcgatcgcgg cctcaacccc cggggcgacc tgcttctcag tcgcccacgg tccgccgggg
     8941 acgaggtgca tgagcgagaa cgtgatgacg gacacggcga agaggatggg gatgagccag
     9001 agaatgcgac ggacaacgta gctggccaac tagaccggta tgttggcggt tacgcgcgac
     9061 ccctgctcgc ggctcgctcg atcacggcgg cggcgatctc ctccggggag cgaagtacgt
     9121 acaacgcggc atatcgctcg tcggggagca ccaacagggc gtcgcgcatg cgctccgcct
     9181 ggtcggtgat cacgtagtcg tcgacgacgc ggtcgggacg gttgcgattg tgcagcgcgt
     9241 actggtcggt cggcagatcg aaggcgatgg tgaccgtggg gatgccgtag cgcgcggcga
     9301 gcctgcggta gccgcttcgg ctggcggcac gcaggttggt ggcgtcgatg acggtcatca
     9361 gacgtcgctt cagccgcccg ttggcgatga gtcccacgac acggaaggct tcggtagacg
     9421 cgccctggtc cgacgggttg tcgctcagca actcccgtgc ccggtcgctc gagaggatct
     9481 ccgtcccgct gaagttacgc gaggcgaacg tggacttgcc cgcgcccgac ggtccgacga
     9541 gcaggacaag ggctgg
//
LOCUS       DATLCO010000159         4119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2453836, whole genome shotgun sequence.
ACCESSION   DATLCO010000159 DATLCO010000000
VERSION     DATLCO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453836"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="VMZ33_03980"
     tRNA            complement(1..76)
                     /locus_tag="VMZ33_03980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:His,seq:gtg)
     gene            137..505
                     /locus_tag="VMZ33_03985"
     CDS             137..505
                     /locus_tag="VMZ33_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16419.1"
                     /translation="MNIPGFIARQFYVSKSLRNTDSGWELQAQNPMGDGTLVGIGHMK
                     VDGNEIAPENVTASRSGELEPIRAQEISRQRPVSVFMGDRVTLAVAGEPLPAGEHKLE
                     VELFELNMGRLAFSIEDRIE"
     gene            713..1402
                     /locus_tag="VMZ33_03990"
     CDS             713..1402
                     /locus_tag="VMZ33_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HUR16420.1"
                     /translation="MLATTRWVRVPPFERGLTLGDFLVPIRVGERATSRQRHLIMVLA
                     GTLLIVLGARISFYVPPNFDVPVSLQTFSVLFGGALLGFRRAIGSVVLYIALGLVGLP
                     VFAYSATTGDYASGMATIASYSNGSLMLGATGGYIIGFLIAAGLVGRLAELGWDRHIG
                     GSIGAMILGNAVIYLIGVPWLMVARGFDLQVGLQYGLWPFVVGDAIKLALAAGLLPVG
                     WWAVQRGSNER"
     gene            complement(1406..1963)
                     /locus_tag="VMZ33_03995"
     CDS             complement(1406..1963)
                     /locus_tag="VMZ33_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HUR16421.1"
                     /translation="MISTSSPVQKTEQLLCIFLDLDTVLLATHQGKYGPELAMQSGVD
                     EPLERLAESAGALVVLIHPHEPDSPRAIDTANRLSALREGLGRRMDGLIIATCPHGED
                     GSCACAKPGTGLIDHVLAEHGLSPEHGWYIGGDQPGVVTGRTAGLKTVRLGPAGEDRL
                     SSIHRPDYEARDLLDASNHIMFAVT"
     gene            2091..2166
                     /locus_tag="VMZ33_04000"
     tRNA            2091..2166
                     /locus_tag="VMZ33_04000"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2124..2126,aa:Arg,seq:tcg)
     gene            2218..3348
                     /locus_tag="VMZ33_04005"
     CDS             2218..3348
                     /locus_tag="VMZ33_04005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUR16422.1"
                     /translation="MIDASSILKQAEEAYSRGEYELAAALFGRLLGNADPTLHITGLL
                     GMADAKYRLDDDEGALQGWIVATQGPETPLTWRAWTALAGARVRQGDLPAAARAYREA
                     ERRAPEYERPGIQSRLGWLNKEMGNAGAAQRYFGRARGGFAPFATYAILGATLAVSLW
                     TGFTAQGQVLENFLALDKEAVMRGELWRLFTVTLVHGGLLHLLFNLYALYIVGPLVEA
                     MYGRTLFVIFYVLSALGGSIASYLFIPGVSVGASGAIFGLFGLIFVSSYIHRPALGAQ
                     ARAMTAQIGMLIAINLVIGFGIGSFGSIDNAAHVGGLLSGAWLGLVVAPRGVAALSSF
                     WQKAKNAVTPARQRYASAIAVVGVAALSIVLALALQVTPFWA"
     gene            3359..>4119
                     /locus_tag="VMZ33_04010"
     CDS             3359..>4119
                     /locus_tag="VMZ33_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16423.1"
                     /translation="MSRSILNVLFGTFTLRFSTALTGGLLAYYLADLHLHGGPEVDAF
                     AFAVIHAAFYVTELVVSPWFGLLSDRLGHHRVMQFGPLFGAVAVLITGVTSNLVVLGG
                     TRILEGAATAASIPSILGFLAMATAGDEGLRGGVSARFELATLAGLGAGIGAAGMFWS
                     FFGPVAFFINAAFYFGSLAIYRYGVRAPDAPAGPHHKPQYGWRRYAKLLRSSHIWLLA
                     PTWIAVNAALGVYAGTGLFNLVRDQSGRYPDQLLAG"
BASE COUNT          632 a         1368 c         1328 g          791 t
ORIGIN      
        1 tggggtggcc aatgggggtc gaacccataa ccttcggatc cacaatccga tgctctgccg
       61 gttgagctat ggccaccgcg ggcgaggagg atcctcgccc gcaattatcg caggaagttc
      121 gcgctaggct ttcgtggtga atatcccggg cttcatcgcg cgccagttct atgtctcgaa
      181 atcgctgcgc aataccgact ccggctggga gcttcaggcc cagaacccga tgggcgacgg
      241 cacgctcgtc ggcatcggac acatgaaggt cgacggcaac gagattgcgc ccgagaacgt
      301 gacggccagc cgatccggcg agctggagcc gatccgggcc caggagatca gcaggcagcg
      361 gccggtgagc gtcttcatgg gcgatcgagt cacgctcgcg gtggcgggcg agccactccc
      421 cgcgggcgag cacaagctcg aggtcgagct cttcgagctc aacatggggc ggctggcgtt
      481 cagcatcgaa gaccgaatcg aataggcgat tgcgtctgac gcaagcaacg gccggaaacc
      541 gagtcgttga gacctccatt gaggccgaat tcggcggaac gttgcgccct atgcaaggag
      601 cgaccgaaat gggcccgctg agggtcggac gccggcggtt acttgcagtg catgcaaccc
      661 ggtcgacggc cgtgacctcg ggtgctggat ttgcgctgat agcatcgaac tcgtgttagc
      721 cactacgcgc tgggtgcgtg tcccaccctt cgagcgcggt ctcacgcttg gcgacttcct
      781 cgtcccgatt cgggtcggcg agcgcgccac ttcgcgtcag cggcacctga tcatggtcct
      841 cgccggaacg ctgctcatcg tcctcggcgc gcgcatcagc ttctacgtgc cgccgaactt
      901 cgacgtgccg gtgtcgcttc agacgttcag cgtgctcttc ggcggcgcgc tgttgggctt
      961 ccgcagagcc atcggatcag tggtgctgta tatcgcgctc ggcctcgtcg ggctgcctgt
     1021 ctttgcctac agcgcaacca ccggcgacta cgcgtccggg atggccacga tcgcgagcta
     1081 ctccaacggc tcgctaatgc tcggcgcgac aggcggatac atcatcggct tcctgatagc
     1141 ggctggcctc gtcgggcgcc ttgcggagct gggctgggac cgccacatcg gcggctcgat
     1201 cggcgcgatg atccttggca acgcagtcat ctacctgatc ggcgtgccgt ggctgatggt
     1261 tgcgcgcggc ttcgacctac aggtcggact tcagtacggg ctctggccgt tcgtcgtcgg
     1321 cgacgcgatc aagctagcgc tggccgcggg tctgctgccg gtcggatggt gggccgttca
     1381 gcgaggctcg aacgagcgct gagccctagg tcaccgcgaa catgatgtgg ttcgacgcgt
     1441 cgagcagatc ccgcgcttcg tagtccggcc gatggatcga cgacagtcga tcttcacccg
     1501 caggccccaa tcgcaccgtt ttgagtcccg ccgttcgccc cgtgacgacg cccggctgat
     1561 ccccgccgat gtaccagccg tgctctgggc tgagcccatg ctcggccagc acgtggtcga
     1621 tcaggccggt gccgggtttg gcgcaagcgc acgagccatc ctccccgtgc ggacaggttg
     1681 cgatgatcaa tccgtccatc cggcgtccca atccctctcg caacgcactc aggcgattgg
     1741 cggtgtcgat ggcccgtggt gagtcaggtt cgtgagggtg gatcagtacg acgagagcgc
     1801 cagcgctctc cgcgagacgc tcgagcggct cgtcgacccc gctctgcatc gcaagctcag
     1861 gcccgtactt gccctggtgg gtggcaagga gaaccgtgtc gaggtcgagg aagatgcaga
     1921 gcagttgctc tgtcttctgg acgggggaac ttgtggagat cacgacaaca ccggactcgg
     1981 atggtaggtc gagctgagca ccagccgcga cagggaggac gcccctcgcc gttaaagagt
     2041 tctttcacct gagcgtggga caatcgctgg gcgctccgag agcgcgctgc gcgcctgtag
     2101 ctcagtggat agagcggatg gcttcggacc atcaggtcgc gggttcgaat cctgccgggc
     2161 gcgccaacct tcctcacagc gaatgcacag ttcgcctgat tagatttgag gcggacgatg
     2221 attgacgctt cttccatcct gaagcaggcc gaggaggcct attcccgagg cgagtacgag
     2281 ctcgctgccg cgctgttcgg gcgcctgctc ggcaacgctg acccgacgct ccacatcacc
     2341 gggctgctgg gcatggccga cgccaaatac cgacttgacg atgatgaggg cgcgctccag
     2401 ggctggatcg tcgcgaccca gggaccagag acaccactga cctggcgcgc ctggacggcg
     2461 ctcgctggcg cacgggtccg ccagggcgat ctgcccgccg ccgcacgtgc ctaccgcgaa
     2521 gccgagcgcc gcgcccccga atacgagcga ccggggatcc agtcgcggct cggctggctc
     2581 aacaaggaga tgggcaacgc cggcgctgcc cagcgctact tcggtcgcgc gcgtggtggc
     2641 ttcgcgccct tcgccactta cgcgatcctc ggcgcgactc tagccgtgag tttgtggacc
     2701 ggtttcaccg cacagggaca ggttctcgag aacttcctgg cgctcgacaa ggaagccgtc
     2761 atgcgcggcg agctgtggcg cctgttcact gtgacgctgg tccacggcgg cctcctgcac
     2821 ctgttgttca acttgtacgc gctctacatc gtcggcccgc tggtggaggc gatgtacggc
     2881 cgcactctct tcgtcatctt ctacgtgctg tccgccctcg gtggctccat cgccagctat
     2941 ctcttcatcc cgggcgtgtc ggtcggagcc agcggcgcta tcttcggact cttcgggctg
     3001 atctttgtgt cgtcgtacat tcatcgcccg gctctgggcg cacaagcgcg cgccatgacc
     3061 gcgcagatcg gcatgttgat cgcgatcaac ctcgtcatcg gcttcggcat cggcagcttc
     3121 ggatccatcg acaacgcggc gcacgtcggt gggctgctct ccggcgcgtg gctcgggctg
     3181 gtcgtggccc cccgcggcgt ggccgcgttg agctcgttct ggcagaaggc gaaaaacgca
     3241 gtgactcctg cgcgccagcg atacgcatcc gcgatagccg tggttggggt cgctgccctc
     3301 agcatcgttc tcgcgctagc gctacaagtc acacctttct gggcctaacc ttcagccggt
     3361 gagccggtcg atcctcaacg tcctcttcgg gacgttcacg cttcgcttca gcacggctct
     3421 cactggcggc ttgctggcct actacctggc cgatcttcat cttcacggcg ggccagaggt
     3481 cgacgcgttc gcctttgccg tcatccatgc cgcgttctac gtgacggagc tcgtcgtttc
     3541 accgtggttc ggcctgctct ccgacaggct cggccaccac cgcgtaatgc agttcgggcc
     3601 gctcttcgga gcggtcgccg tgctcatcac tggtgtcaca agcaacctcg tcgtcctcgg
     3661 cgggacgcgc atcctcgaag gtgccgcaac agccgcgagc attccgagca tcctcggctt
     3721 cctggccatg gccacggccg gcgacgaggg tctccgcggc ggggtctcgg cgcgcttcga
     3781 gctggccacc cttgcaggtc tcggcgcagg catcggggca gcgggcatgt tctggtcgtt
     3841 cttcggcccg gtcgcgttct tcatcaacgc cgcgttctac ttcggttcgc tcgcgatcta
     3901 ccgctatggc gtccgtgccc cggacgcgcc tgccggccct caccacaagc cgcagtacgg
     3961 ctggcgtcgc tacgcgaagc tcctgcggtc atcgcacatc tggctgttgg cgccgacgtg
     4021 gatcgccgtc aacgccgcgc tcggcgttta tgccggcacg gggctcttca acctcgttcg
     4081 cgaccagtcg ggtcgctacc cggaccagct cctcgctgg
//
LOCUS       DATLCO010000160         4818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3942525, whole genome shotgun sequence.
ACCESSION   DATLCO010000160 DATLCO010000000
VERSION     DATLCO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3942525"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VMZ33_04015"
     CDS             complement(<1..476)
                     /locus_tag="VMZ33_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HUR16424.1"
                     /translation="MTSKGFADAVIVAAGASSRMGGLDKLTETLLGRPLLQWSVDALS
                     AAESVARLIVVARADRVEELTAATWLRGATVVPGGDRRSDSVRAGVDAATADVVLVHD
                     GARPLASSSLADAVARAAAEHGAAVPAVAVVDSLKRAADGRLTDSVWRDGLVRAQTP"
     gene            complement(473..1531)
                     /locus_tag="VMZ33_04020"
     CDS             complement(473..1531)
                     /locus_tag="VMZ33_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HUR16425.1"
                     /translation="MINFVRLFGGALGALIAVVLADSFDIPRADLASLGGVVLLAGWV
                     SSWFVIGFSILPHITVNPARWLIAHVMDLSTTEFISATGGLLIGLLIGILLSVPMSNL
                     PDPYRWVLPLGTVVVTGLGMMGLTVAKRQDLVGAMRNAGFIRTPDRSAGSYVNDGPIT
                     YVDTSALIDGRITDVVASGFLQGTLVVPRFVIDELQSIADNRDQARRVRGRRGLEVLA
                     VLQKDPRVGVELTDEDPANVATVDAKLIELAKARGGGVLTNDFNLNRVAQLHDVRVMN
                     LNHLANALKPAFLPGDGIRVKVIQQGKEPGQGLAYLDDGTMIVVEGGGKLIESEVDVT
                     VTRVLQTVAGRMVFAQPR"
     gene            complement(1528..2982)
                     /gene="radA"
                     /locus_tag="VMZ33_04025"
     CDS             complement(1528..2982)
                     /gene="radA"
                     /locus_tag="VMZ33_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HUR16426.1"
                     /translation="MARTETRYVCGTCGATSIRWEGQCRSCAGWNTLVESLVTKGPNK
                     SAHRSATPAPPTTALANVDAGDTYRLPLGIREVDRVLGGGLVPGSLILLGGEPGIGKS
                     TLVLSVAAAVAASDPEARVLYASGEESAGQIRLRAARLGVVGAATSAGDRIDVLTETS
                     VERIVAAAQSSAPALLIVDSIQTLTADDLDGPQGSVGQVRSSAARLQSYAKESGVPLV
                     LVGHVTKDGSLAGPKTLEHLVDVVLTLEGERLSGLRLLRSSKNRFGSTEEVGVFEMTA
                     DGLAEVADPAAAFIEPDSLGAPGVAVAATLEGSRPLLVEVQALVTPAYGGSPRRTVSG
                     LDPQRLALLIAVLGRRAGFNLAGHDVYASVVGGLSVDEPAIDLPLAVALASALRDKPL
                     PTGTVLCGEIGLTGELRPVSGLERRLREAARLGFVRAIVPERNARGATEAPPSGLTVI
                     AVPTLRDALVKVLGMSEGRVPTAETVALKVGVDA"
     gene            complement(2988..>4818)
                     /locus_tag="VMZ33_04030"
     CDS             complement(2988..>4818)
                     /locus_tag="VMZ33_04030"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HUR16427.1"
                     /translation="TAIAEGLAQRIVAGDVPETLLNKRVLTLDIGSLVAGTKYRGEFE
                     ERLKKIIEELRNTNDAVLFIDELHTLVGAGAAEGAIDAANILKPPLARGELQCIGATT
                     LDEYRKYIERDAALERRFQPVMVEEPTLEQTIEILFGIRGRYEEHHKVKITDEAVRAA
                     ADLSIRYITDRHLPDKAIDLIDEAASRVRLRTGSLPPELKAAQKEFDRVTKEKEAAIN
                     DQDYEVAANLRDAEATAKENIDTLRADWQATQANDVASVTDEDIAQVVAMWTGIPVTR
                     IAQEESDRLLKMEDALREKVIGQDEAIDTIAKAVRRARAGLKDPKRPIGSFIFLGPTG
                     VGKTWLAKALAEFMFGSEDALIKIDMSEFMERHNVSRLVGAPPGYVGFDEGGQLTEAV
                     RRKSYSVVLLDEIEKAHPEVFNILLQILEDGHLTDAKGRRVDFRNTVIIMTSNVGAKS
                     LLKDTSLGFRPVAVDETKAEQQQYDRMREKVLDQLKTQFRPEFLNRVDSVVVFRSLQI
                     EQIRVIVELLLDRVRVQLKAQQIDLEITQEAKDHIIRLGYDIDYGARPLRRVIQNMVE
                     DQLAEALLMGRFVAGMTVRVDKSEDTGLTITPIEEKVPVEAGA"
BASE COUNT          808 a         1607 c         1580 g          823 t
ORIGIN      
        1 ggcgtctgtg cgcgcacgag gccgtctcgc catacagaat ccgtcagccg tccgtcggca
       61 gcccgcttga gagagtcgac aacggccacg gcaggcacgg ccgcgccatg ttcggcggcg
      121 gcgcgcgcga ctgcgtcggc gagggaactc gatgccagcg gtcgcgcgcc gtcgtgtacc
      181 aggaccacgt cggcagtggc cgcgtcaacg ccggcgcgaa cggaatccga tcgccggtcg
      241 ccccctggga caactgttgc gccacggagc caggtggcgg cggtcagctc ctcaactcgg
      301 tcagcgcgag ccacgacgat cagtcgtgcg accgactctg cggccgaaag cgcgtcaacc
      361 gaccactgga gcaacggccg cccgagcagt gtctcggtga gcttgtccag cccgcccatt
      421 cgtgagctgg cgccggcggc gacgatcacc gcatcggcga atcccttact tgtcaacgcg
      481 gttgggcgaa gaccatgcgc cccgcaaccg tctgcaggac gcgcgtcacg gtcacgtcga
      541 cctcactctc gatgagcttg ccgccacctt caaccacgat catcgtcccg tcgtcgaggt
      601 acgccagccc ctggccgggc tccttgccct gctggatgac cttgacgcga atcccgtcgc
      661 cgggcaggaa cgccggcttc agtgcgttcg ccaggtggtt caggttcatg acgcgaacgt
      721 cgtgaagctg cgcgacgcgg ttgaggttga agtcgttggt gagcactccg ccgccccgcg
      781 ccttggccag ctcgatgagc ttggcgtcga ccgttgccac gttggcggga tcctcatcgg
      841 tcagctcgac gccgacgcgc ggatccttct ggagaacagc gaggacctcg aggccgcgcc
      901 gaccgcgaac gcgccgcgcc tgatcgcggt tgtcggcgat cgattgcagc tcatcaatga
      961 cgaagcgggg cacgacgagc gtgccctgca ggaaaccaga ggcgaccacg tccgtgatcc
     1021 gtccgtcgat tagggcgctg gtgtcgacat atgtgatcgg cccgtcgttc acgtaggagc
     1081 cagccgatcg gtcaggcgtc cgaatgaaac cggcgttgcg cattgcgccc acgaggtcct
     1141 ggcgcttggc gacggtcaac cccatcatcc cgaggccagt cacgacgacc gtgcctagcg
     1201 ggagcaccca gcgatacggg tcgggcaggt tgctcatcgg caccgacaac aggatgccaa
     1261 tcaacagacc gatcagcagg ccgccggtag cgctgatgaa ctccgtcgtc gacagatcca
     1321 tgacgtgtgc gatcagccag cgtgccgggt tgacggtgat gtgcggcagg atggagaagc
     1381 cgataacgaa ccaggaactg acccaaccgg ccagcaggac gactccacca aggcttgcca
     1441 ggtcagcgcg cgggatgtca aagctgtcgg ccaacaccac cgcgattagc gcgccgagcg
     1501 caccgccgaa aagccggaca aagttgatca tgcgtcgacg ccgaccttga gcgcaaccgt
     1561 ttcggcggtt ggtacccgac cttcgctcat gccgagaacc ttgacgagcg cgtcgcgcaa
     1621 cgtgggcacg gcaatgaccg tgagtccgct gggtggcgct tccgtggccc cgcgtgcgtt
     1681 gcgctcgggg acgatggcac gcacgaagcc cagccgcgcc gcttcgcgaa ggcgccgctc
     1741 cagccccgag accggacgga gctcgccggt caagccgatc tcgccgcaca gcaccgtgcc
     1801 cgtggggagt ggcttgtcgc gcagcgccga ggcaagggcg acggctaacg gcaggtcgat
     1861 ggcgggctca tcgacggaca ggccgccgac gacactggcg tagacgtcat ggccggccag
     1921 gttgaagccg gcacgtctgc cgagaaccgc gatgagcagc gccagccgct gcggatcaag
     1981 gccggacacg gtccggcgcg gcgatccccc gtaagccggc gtgacgagtg cctggacttc
     2041 gacgagcagc gggcggctgc cctccagcgt ggccgcgacg gcaaccccgg gcgcgccaag
     2101 cgagtcaggc tcgataaagg cggcagccgg gtcggcgacc tccgccaagc catcggcagt
     2161 catctcgaac acgccgacct cctcggtcga cccaaaccgg ttcttgcttg atcggagcag
     2221 tcgcagcccg gaaagccgct cgccctcgag tgtcaatacc acgtcgacca gatgttccaa
     2281 cgttttggga cctgccaacg acccatcctt ggtcacgtgc ccgacaagga ccagcggcac
     2341 gccactttcc ttggcatagg actggagacg cgcagcactc gagcgcacct gcccgacgct
     2401 cccctgtggt ccgtcgagat catcggcggt caatgtctgg atcgaatcga cgatgagcag
     2461 cgccggcgcc gagctctgcg cggcggctac gatgcgttca accgaagttt ccgtgaggac
     2521 gtcgatgcgg tcgccagcgc tggttgcggc acccacgaca cccagcctgg ccgcccgtag
     2581 gcggatctgt cctgccgatt cctcgcccga ggcgtagagc acgcgcgcct ccggatcgga
     2641 cgcggcaacg gccgcggcca cgcttagaac gagcgtcgac ttgccgatgc ccggctcacc
     2701 accgagcagg atcagcgacc ccgggacgag gccgccgccg agtacgcgat ccacctcgcg
     2761 gatgcccagc ggcagccgat aggtgtcacc ggcatcgaca ttcgccaggg cggttgttgg
     2821 tggcgccggc gtcgcgcttc ggtgggcgct tttattcggg cccttggtta cgagggattc
     2881 aacgagcgtg ttccaaccgg cgcaggatcg gcattgacct tcccaccgga tgctcgtcgc
     2941 gccgcacgta ccacagacgt agcgcgtctc ggttcgcgcc atgagcctta agcgcccgct
     3001 tcgaccggaa ccttttcctc gatcggcgtg atcgtcagcc cggtgtcttc agacttgtcg
     3061 acgcggaccg tcattcccgc cacgaagcgg cccatgagca gggcctcggc gagctggtcc
     3121 tcaaccatgt tctgaatcac gcgccgcaag ggccgcgcgc cgtagtcgat gtcatagccc
     3181 agccggatga tgtggtcctt ggcttcctgc gtgatctcga ggtcgatctg ctgcgccttg
     3241 agctgcaccc gaacccggtc gagaagaagc tcgacgatga cacggatctg ctcgatctga
     3301 aggctgcgga agacgacaac cgagtcgaca cggttcagga actcgggccg gaactgggtc
     3361 ttgagctggt cgagcacctt ctcgcgcatg cggtcgtact gctgctgctc ggccttggtc
     3421 tcatcgaccg cgaccggtcg gaagccgagg ctggtgtcct tgagcagcga cttcgcgccg
     3481 acgttgctgg tcatgatgat gaccgtgttg cggaagtcga cacggcgacc cttggcgtcg
     3541 gtgaggtgcc cgtcctccag gatctggagc aggatgttga acacctcagg atgggccttc
     3601 tcgatctcat cgaggagcac cacgctgtag cttttgcgcc gcacagcctc cgtcaactgg
     3661 ccgccctcgt caaagccgac gtagcctggc ggcgcaccca cgagacgcga gacgttgtga
     3721 cgctccatga actcgctcat gtcgatcttg atgagcgcgt cctcgctgcc gaacatgaac
     3781 tcggcgagcg ccttggccag ccacgtcttt ccgacaccgg tggggccaag gaagatgaat
     3841 gagccgattg ggcgcttggg atccttcaga ccggcgcgag cgcggcggac cgccttggcg
     3901 atcgtgtcga ttgcctcatc ctggccgatg accttctcgc gcagcgcatc ctccatcttc
     3961 agcaggcgat ccgactcctc ctgggcgatg cgcgtgaccg ggatgcccgt ccacatggcc
     4021 accacctggg cgatatcttc gtcggtgacg ctggccacgt cgttggcctg cgtggcctgc
     4081 cagtcggcgc ggagcgtgtc gatgttttcc ttggcagtcg cctcggcgtc gcggaggttg
     4141 gcagcgacct catagtcctg gtcgttgatc gccgcttcct tctccttggt gacgcggtcg
     4201 aactccttct gagcagcttt gagctccggc ggaagtgagc ccgtgcgcag ccgaacgcgg
     4261 ctcgcagctt catcgatgag gtcgatcgcc ttgtcgggga ggtggcggtc ggtgatgtag
     4321 cgaatcgaca ggtcggcagc ggcgcgcacg gcctcgtccg tgatcttgac cttgtggtgc
     4381 tcctcgtacc gtccgcggat cccgaagagg atctcgatgg tctgctcgag cgtgggctct
     4441 tcaaccatga ccggctggaa gcggcgctcg agggcagcgt cgcgctcgat gtacttgcgg
     4501 tactcgtcga gcgtggtcgc gccgatgcac tgcagctcgc cccgcgcgag gggcggcttg
     4561 aggatgttcg cggcatcgat cgcaccctcc gcggcgcccg cgccgacaag ggtgtgcagc
     4621 tcatcaatga acagcacggc gtcgttggtg ttgcggagct cctcaatgat cttcttgagt
     4681 cgctcctcga actcgccgcg gtacttcgtg ccggcgacca gtgagccgat gtccagggtc
     4741 aggacgcgct tgttcagcag cgtctccggg acgtcgccgg cgacgatgcg ctgggcgagc
     4801 ccctcagcga tggcggtc
//
LOCUS       DATLCO010000161         3735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_221742, whole genome shotgun sequence.
ACCESSION   DATLCO010000161 DATLCO010000000
VERSION     DATLCO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221742"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VMZ33_04035"
     CDS             complement(<1..874)
                     /locus_tag="VMZ33_04035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUR16428.1"
                     /translation="MSEKRASWRFKDGDEIVPGRLAVDVLGGGIRAEAWLAWDTTLHT
                     IVVVKLLRPDFVANERARTALAREVRALERLAHPAIVRMFDSSLDGERPFIVLESLDG
                     PRLSTLLRRYGPLTPEQLVPLGLEVASALAYLHNLDLVHLDVKPKNIIMSASPKLIDL
                     GLVRPLSEVRALTSALGTTAYMSPEQSQDETIAGFGPLGDVWGLGVTLYEAASGRLPF
                     PRSTDEDKHPQVHVGPAPLPKKIPEVLRGVIMPMLAYQPEERPTAKEVAARLEDLVEE
                     ARLVARKRLHARHRR"
     gene            complement(871..2067)
                     /locus_tag="VMZ33_04040"
     CDS             complement(871..2067)
                     /locus_tag="VMZ33_04040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HUR16429.1"
                     /translation="MNVVDIIALVMIGLALVFGWRSGFVIQALALVGFVLGIGALVLV
                     APSATELVADMEPWLRTLLVLSILAGVVLLAQGIGSTIGAALRRRMGSGLLSGIDQGL
                     GAAFGLARGVFLVWLIGGLVGLLPFPTLAAEAKQSLVLRTLDTRLPSPVVLAADLGRL
                     LEAAGLPDVLVGAPLPIDVPADAPSGDEAEAIAAHAGASTVRVESTACGLFLTGTAFA
                     VTRDHFVTNAHVVAGSDKTWISFDGSLDRFSASVVDFDPNLDVALLYAPDVHVRPLTL
                     APSLPRRGNNAAAIGFTGGGRQRLIPGVISRTLSALGRDIYGQAVAPREVIEMRLDVS
                     PGDSGGPVLLESGEVGGVTFSESRVDRSIGYALSPRAVATSIDDSLGTSDPVPTGNCV
                     SNISTG"
     gene            complement(2064..2531)
                     /locus_tag="VMZ33_04045"
     CDS             complement(2064..2531)
                     /locus_tag="VMZ33_04045"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUR16430.1"
                     /translation="MAAGPALVPWQKDLLDLVRRAVLATIAADGHARLVPIAFIWVTG
                     HSKLPMLYSALDDKPKSVVDPRDLARVHDIGQRPRVTVLVDHWSEDWYELAWLRLEGN
                     ASVLEPTANNTTERSVAIAALRAKYPQYATHWLEEAPLIRIAVDRLTGWRSNP"
     gene            complement(2860..3363)
                     /locus_tag="VMZ33_04050"
     CDS             complement(2860..3363)
                     /locus_tag="VMZ33_04050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUR16431.1"
                     /translation="MKSYSSPPSMTIDTARRYKVTLHTSAGDMTAELFPTDAPNTVNN
                     FVFLARDGFYDGVIFHRVIPGFMIQGGDPTGTGSGGPGYKFRDEPVSKKYERGILAMA
                     NAGPNTNGSQFFVMHADYGLPPSYTIFGRLTDGHDTLDKIAGAQTGAQDRPTSPVTID
                     SVEVSEE"
BASE COUNT          670 a         1204 c         1236 g          625 t
ORIGIN      
        1 accggcggtg ccgggcgtga agacgcttgc gggcgaccag ccgcgcctcc tcaacgaggt
       61 cctctaaccg tgcggcgact tccttggccg tcggtcgctc ctccggctga taggcgagca
      121 tgggcatgat cactcccctc aacacctcgg gaattttctt gggtagtggt gccggaccca
      181 cgtggacctg tgggtgctta tcctcgtcgg tcgaacgcgg aaacgggagc cgccctgatg
      241 ccgcctcgta cagggttacg ccgagacccc acacgtcacc cagcggcccg aatccggcaa
      301 tcgtttcgtc ctggctttgt tcaggtgaca tgtaggcagt cgtaccgagg gccgacgtga
      361 gggcgcgaac ctcactcaaa gggcgaacca gtccaaggtc tatcagcttg gggctggcgc
      421 tcatgatgat gtttttcggc ttgacgtcga gatgcaccaa atcgaggttg tgcaggtagg
      481 cgagcgcaga agcaacctcg aggccgagtg gtacgagttg ctcaggcgtc agcggaccgt
      541 agcgtcggag cagagttgat agacgcggcc cgtccagtga ctcgagaacg atgaacgggc
      601 gctcgccgtc aagcgatgag tcgaacatcc ggacgattgc cgggtgcgcc agccgctcca
      661 gggcacgtac ctcgcgagcg agtgccgttc tggcgcgctc gttggcaacg aagtcgggcc
      721 gcaggagctt gacaaccacg atggtgtgca acgtcgtgtc ccaggccagc caggcctcgg
      781 cacggatccc accgccgagg acgtctacgg caagacggcc cggaacgatc tcatctccgt
      841 ccttgaaccg ccagctggct cgcttctcgc tcatccagtg ctgatattgc ttacgcagtt
      901 gccagttggc accgggtcac tggtaccaag cgagtcgtct attgaagtgg caactgccct
      961 tggcgacagc gcatacccga tcgatctgtc tacgcgtgac tcggagaaag tcactccacc
     1021 aacctcgccc gactccagca gcaccggccc gccgctgtcg cccggcgaaa cgtcgagccg
     1081 catctcgatg acctcgcgcg gggcgacggc ctggccgtag atgtcgcgac cgagggcgct
     1141 caacgtcctg ctgatgactc ccgggatgag ccgctgacgt ccgccgcccg tgaagccgat
     1201 cgcggcggcg ttattgccgc gccgcggaag cgagggtgcg agtgtcagcg gtcgcacgtg
     1261 gacgtcgggc gcgtagagga gcgcgacatc caggttgggg tcgaagtcga cgacgcttgc
     1321 cgagaaacgg tcgagcgagc cgtcgaagga gatccatgtc ttgtccgagc cggccacgac
     1381 gtgcgcattg gtcacgaaat ggtcgcgcgt caccgcaaaa gccgtgcccg tgaggaacag
     1441 accgcaggcc gtggactcga cgcgaacggt gctggcaccg gcgtgcgcgg caattgcctc
     1501 tgcctcgtcg ccactgggcg cgtcggccgg aacgtcgatg ggcagcggcg cgccaacgag
     1561 cacgtccggc agcccggccg cttccagaag gcgccccaaa tcggcggcga ggaccaccgg
     1621 cgaaggcaag cgcgtgtcga gagtgcgtag cacgaggctc tgcttcgctt ccgcggcgag
     1681 cgtcgggaag gggaggaggc cgaccagccc gccgataagc cacaccagga acactccacg
     1741 cgccaggcca aatgcagcgc cgaggccctg gtcaatgccg ctcagcaatc cgctacccat
     1801 gcgccgtcgc agcgcggcgc cgatggtgct gccgatgccc tgtgccagca acacgacgcc
     1861 ggcgaggatg ctcagcacga gcaacgtccg gagccacggt tccatgtccg cgacaagttc
     1921 cgtggctgat ggcgcgacga gaacgagggc gccgatgccg agcacgaagc ccaccagggc
     1981 aagcgcctgg atcacgaagc cggaacgcca accgaagacg agggccaggc cgatcatcac
     2041 cagggcgatg atgtcgacga cgttcatggg ttcgaccgcc agccggtcag gcggtcgacc
     2101 gcgatcctga ttagcggcgc ttcctcgagc caatgtgtcg cgtactgcgg gtacttggcc
     2161 cgtagagcgg caatggccac gctgcgctct gtcgtgttgt tggcggtggg ctcgagcacg
     2221 ctcgcgttgc cctcgaggcg cagccacgcc agctcgtacc agtcctcgct ccagtgatcg
     2281 acgaggacgg tcacgcgcgg tcgctgcccg atgtcatgga cgcgtgcgag gtcgcgcggg
     2341 tcaacgacgg actttggctt gtcatcgagg gccgagtaga gcattggcaa cttggaatgg
     2401 cctgtgaccc agatgaaggc gatcggcacg aggcgtgcgt gaccgtcggc ggcgatcgtc
     2461 gccagcacgg ctcgtcgcac taggtcaagc aagtccttct gccagggaac aagggccggt
     2521 cctgccgcca taggccgagg ctaccgtcgc gcgcagacga aacgtaccga cattgcttgc
     2581 gcctcccacc gtttgtaatg actggtcagc cttaagtcga caccggagtc gcacgaaccg
     2641 ccagaaccgc gccaaccagg cgattctcag cacgaccgtt agaggcgacc ggctgagcca
     2701 ccccggttcc tcaacttaag gccgacctgg ccgtacacct gacgggacat acgccgcggg
     2761 acgtcaggcg atcgggtaga cgaacggccg ccacatcgac gcggtcgatc gcttcctgat
     2821 cctcgtccgt agcgccctga tggggaatgg cttcgctgcc tactcctcgg acacctcgac
     2881 gctgtcgatg gtgacgggcg aggtcggccg gtcctgggcg ccggtctggg cccccgcgat
     2941 cttgtcgagc gtgtcgtgcc cgtctgtcaa tcggccgaag atcgtgtagc tgggcggcag
     3001 tccgtagtcg gcatgcatga cgaagaactg gctgccgttg gtgttcgggc ccgcgttcgc
     3061 catcgcgagg atgccacgct cgtatttctt gctgacaggc tcgtccctga acttgtagcc
     3121 gggtccgccg ctgccggtgc ccgtggggtc accgccctgg atcatgaaac ccgggatcac
     3181 cctgtgaaag atcacgccgt cgtaaaagcc gtcgcgggcc aggaaaacga agttgttgac
     3241 cgtgttcggc gcgtcggtcg ggaagagctc ggcggtcatg tcgccggcgc tcgtgtggag
     3301 cgtcaccttg tagcggcgcg cggtgtcgat cgtcatcgag gggggagagg agtaggactt
     3361 catgcgtcaa gtatggccgc tgcatcggag atgcaatatc aacgaacctg cctgcggcgc
     3421 gcggcgatga tcgcgagctg cacgatctgt ccatgcagaa accggtcatg ttggccgtcg
     3481 tcgtgcgccc ggacggacga cgcgccacgt tcccacttcg cgcggagcag ccgcggtcga
     3541 taagcgggct aatcgcagat ctcctgcgcc gactcttcga gacccgctag cacttctgcc
     3601 tagaccgagc gcggcccgac cccgcgctcg ctggtagcct tccggctgtc catgagtgca
     3661 gaaatttcgg gttcgcgcga ccagttcgag tcggcaatga ccgagggcta cgccctcgag
     3721 cagccctcga tcacc
//
LOCUS       DATLCO010000162         3694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_968290, whole genome shotgun sequence.
ACCESSION   DATLCO010000162 DATLCO010000000
VERSION     DATLCO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968290"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="VMZ33_04055"
     CDS             <1..1960
                     /locus_tag="VMZ33_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HUR16432.1"
                     /translation="LVTRDARGNSRLWDLVDFSDSDNAWAAATQRAAVDAQYYASKTD
                     GWYLAEMGFDLLAEHYLPSPVEGQPAMLQCDLPAITSVVHYDDTPGDGFGYDNAFWHP
                     EREIMVYGDGDGVETGPFSGAQDVVSHELSHAVTTCRAPLTYYSSSGALNEGFSDIMA
                     AAMEWEFAEPNSSGCRRLTSQTACPDWWIGEDSIITGTNFGFRNLARPQSTYNNPSHW
                     ADRYRGSFDNRGVHYNSLLPGHAFYLMSMGGRNARCVGATDTKADCDVVVPSIGIEDA
                     AHLFFNAWGTLPEDADYCDAQAATIVAAQTVFPGSDLHLASAVLGWAAVGPRTDYPNA
                     IGNACSGNLPKLTLNTRSVALAGGQSAAIDVTNLGQFEALSVSAQGPVTAELVASDPD
                     TVLVSVPEGTAPGAYYLMFTGTTASVTTTRYAVGTVVVDATPPTASVTQVALIGSGQI
                     AQSGTIQLQVSWSAADDLSGVADADLEWTTTGSEPWPVLVGTPPANGQSAVIVGGNEY
                     SFRVRALDGVGHETLSQESGPWRIERSQENAATYTKAWIDRAVADYWGGGSRSAKAKG
                     ATATFSFNGTAVQWITNRGQKRGKAKIYLDGVFVTRVDLYSSSASNRRIGYTVNGLTD
                     GNHTLIIWVMGTTNRPRVDIDGFVVLDR"
     gene            complement(1957..2184)
                     /locus_tag="VMZ33_04060"
     CDS             complement(1957..2184)
                     /locus_tag="VMZ33_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16433.1"
                     /translation="MDRLKSENPEADPTPIDESLPLHHAGEGEVLEDPVEDASADSFP
                     ASDPPSWTGSSAASDPSADVTPVDDQTGDKP"
     gene            2215..2289
                     /locus_tag="VMZ33_04065"
     tRNA            2215..2289
                     /locus_tag="VMZ33_04065"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2247..2249,aa:Lys,seq:ctt)
     gene            complement(2358..2951)
                     /locus_tag="VMZ33_04070"
     CDS             complement(2358..2951)
                     /locus_tag="VMZ33_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16434.1"
                     /translation="MPIHTRTRRTRLALGLAIALISASVVVPTVLAAPTQNGHGSAAV
                     CRYQETDPADINGNTGFRLTRIAIDPPKLFGTYPGQMVGWRFVVQRHYDDNDPYWNGP
                     WVRIFASRTQKAVANPDASAAFSKMFAKINYVDSVDRWPNGYHTVFRAIATFYWYNAD
                     GSVETSERFNMGVYPVYREGVYQFTSYGSCEGSWLDS"
     gene            3179..>3694
                     /locus_tag="VMZ33_04075"
     CDS             3179..>3694
                     /locus_tag="VMZ33_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16435.1"
                     /translation="MRNALKSVLSGSMVLMAFLLAAAPVAADTELGHNDPVGQHSLRD
                     FDFDHTGATCTYKSLANGPRGYENLLKRIDVRPPRMRSIGARQRVGWRFMVQRSLDQE
                     IWKTTYKSPTQKATAQAGVNAAFGPMGVQVAVPANSYNSHIYRVRVTMFWYAADGSVQ
                     GKARHEVDYYGK"
BASE COUNT          649 a         1191 c         1182 g          672 t
ORIGIN      
        1 actcgtcaca cgcgacgcaa gaggcaactc ccggttgtgg gatttggtcg acttcagtga
       61 ctcagacaac gcatgggccg cggccaccca gcgcgcggct gtggatgccc agtactacgc
      121 gtcgaagacc gacggttggt acctggctga gatgggcttc gaccttctag ctgagcacta
      181 cttgccgagt ccggtcgaag gccagccggc catgctgcaa tgtgacctgc cggcgatcac
      241 gagcgtcgtg cactacgacg acacacccgg cgacggcttc ggctacgaca acgccttttg
      301 gcatccggag cgcgagataa tggtctacgg cgacggtgac ggcgtcgaga ccgggccatt
      361 ctcgggcgcc caggatgttg tgtctcatga gctgagccac gcagtcacca cctgccgcgc
      421 gccgttgacc tactactcgt catcaggtgc cctgaacgaa ggcttctccg acatcatggc
      481 cgccgccatg gagtgggagt tcgccgagcc caacagttcg ggctgccgtc ggctgaccag
      541 ccagaccgca tgtcctgatt ggtggatcgg cgaggactcg atcatcacgg gcacgaactt
      601 tggcttccgt aacctcgcgc ggccgcaaag cacctacaac aacccgtcgc actgggccga
      661 ccgctaccgc ggctcattcg acaaccgcgg cgtccactac aacagcctgc tgccgggtca
      721 cgccttctac ctgatgtcga tgggcggccg aaacgcgcgc tgcgtgggcg cgacagacac
      781 gaaggccgac tgcgacgtcg tggtgccgtc catcgggatc gaggatgcgg cccacctttt
      841 cttcaacgcg tggggcaccc ttcctgagga cgcggactac tgcgatgccc aggctgcgac
      901 catcgtggcc gcccaaaccg tctttcctgg ctcggatctc cacctcgcgt cagcagtcct
      961 cggttgggcg gcggttgggc cgcgtaccga ctatccgaac gccatcggca acgcttgctc
     1021 gggcaatctg cccaagctga ccctcaacac aaggtccgtg gcgctagctg gcggccagtc
     1081 cgcagcgatc gacgtcacca atctcggcca gttcgaggca ctttccgtgt ctgcccaagg
     1141 gccggtaact gcggagctcg tggcatctga ccctgacacc gtcctcgtat ccgtgcccga
     1201 gggtacggcg cccggcgcct actacctcat gttcacgggc acgaccgcgt cggtcacgac
     1261 gactcgctat gccgttggga ctgtcgtcgt cgacgccact ccgccgacag ccagcgtgac
     1321 gcaggtcgcc ctgatcggtt cgggtcagat tgcccagtcg ggcacgatcc aattgcaggt
     1381 gtcctggtcg gcagcggatg acctgagcgg tgtcgctgac gcggatctgg aatggacgac
     1441 caccggcagc gagccgtggc cggtgttggt cggcacgccg cccgccaatg gacagagcgc
     1501 cgtcatcgtt ggcgggaacg agtacagctt ccgcgttcgg gcgctcgatg gcgtcggcca
     1561 cgaaacgctg tcgcaagagt ctggcccgtg gcggatcgag cgctcgcagg agaacgccgc
     1621 gacttacacg aaggcctgga tcgaccgggc cgtggcggac tattggggcg gcggctccag
     1681 gtcggccaag gcgaagggcg ccactgcgac cttcagcttc aacggcacgg cggtgcagtg
     1741 gatcacgaac cgcgggcaga agcgcggcaa agccaagatc taccttgacg gcgtgttcgt
     1801 cacccgcgtc gacctgtatt catcgtccgc ttcgaatcgc cgcattgggt acacggtcaa
     1861 cggcctgacc gacggtaacc acacgctgat catctgggtg atgggaacca ccaaccggcc
     1921 gcgcgtcgac atcgacgggt tcgtcgtcct cgaccgttag ggcttatctc ccgtttggtc
     1981 gtcgaccggc gtcacgtcgg cagagggatc gctggccgca gacgatccgg tccagctcgg
     2041 gggatcgctg gcggggaagg aatcggctga ggcgtcctct accggatcct cgagcacctc
     2101 gccctcgccg gcatgatgga ggggaagcga ttcgtcgatc ggggtcggat ccgcttcggg
     2161 gttctctgac ttcagcctgt ccacgcacgc tcctggggta gaattggggt tcgtgggccg
     2221 ctagctcaat ggtagagcag ctgactctta atcagctggt tgaaggttcg agtccttcgc
     2281 ggctcaccag cattctccgc acgatgaagc ccctcaggca atgccgaggg gctttcagtt
     2341 tgtggccgta cgtcaggtca gctgtcgagc caggagccct cgcaacttcc gtacgaagtg
     2401 aattggtaga cgccttcgcg gtaaacgggg taaacgccca tgttgaagcg ttcgctggtt
     2461 tccaccgagc cgtcggcgtt gtaccaatag aaggtcgcga tggcgcggaa gacggtgtgg
     2521 tagccgttcg gccaccggtc gaccgagtcc acgtagttga tcttggcgaa catcttgctg
     2581 aacgccgccg atgcgtccgg gttggcgacg gccttctgcg tgcggctcgc gaagatgcgt
     2641 acccagggtc cgttccagta cgggtcgttg tcgtcgtagt gacgctgcac gacgaaccgc
     2701 cacccgacca tctgacccgg gtaagtgcca aagagcttcg gcggatcaat ggcgatccgg
     2761 gtcaacctga accccgtatt gccgttgatg tccgcggggt ccgtttcctg gtagcggcac
     2821 accgccgccg atccgtgccc gttctgggtt ggcgccgcaa ggacggttgg gaccaccacg
     2881 gaggcgctga tcagcgcgat tgcaaggccg agcgccaagc gcgttcggcg tgtgcgtgtg
     2941 tggattggca tgggttcgcc tcgcttcccc gtctggcgcc gctgggcagt ggcgcatcag
     3001 ggcgccattg gattctccct ccgtgcattc cacatccgcg ctgtcacgca cgaccccacc
     3061 caatccccgg tacgtggcta ctgagcgacg agtccgtgga caacgcagtg gtggctcgga
     3121 tggtggaggc cacgatgccc ggtagggtgc cttcagataa ttccataagg agttggacat
     3181 gcgcaacgct ctcaagtccg ttctatcggg gtccatggtg ctcatggcct tcctcctggc
     3241 ggcagcgccc gtcgccgccg acaccgagct cgggcacaac gaccctgtgg ggcagcactc
     3301 gctgcgcgac tttgatttcg accacaccgg agcgacgtgc acctacaaga gcctcgcgaa
     3361 cggcccgcgc ggctacgaga atctgctcaa gcgcatcgac gtacggccgc cgcgcatgcg
     3421 gtcgatcggc gctcggcaac gcgtcggctg gcgcttcatg gtccagcgct cgctcgatca
     3481 ggaaatctgg aagacgacct acaaaagccc gactcagaag gcgaccgctc aagccggcgt
     3541 caatgccgcg ttcggcccga tgggcgtgca agtggcggtt ccggccaact cgtacaacag
     3601 ccacatctac cgggtccgcg tgacgatgtt ctggtacgcc gctgacggca gcgtccaggg
     3661 caaagccagg cacgaggtcg attactacgg aaaa
//
LOCUS       DATLCO010000163         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_969188, whole genome shotgun sequence.
ACCESSION   DATLCO010000163 DATLCO010000000
VERSION     DATLCO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969188"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VMZ33_04080"
     CDS             <1..751
                     /locus_tag="VMZ33_04080"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16436.1"
                     /translation="RKLARWINRTVDLQRLTAVLPISKREYTVFRPDDVSRESLFEAG
                     RADPDKQMPYWAKVWPSGVALADVVVERREEITGRQILELGAGLGVTASAVLEYGGDL
                     VTADYSALPLAHCRLTTLVNTGRAPEATCFNWRHDAEIRAVMALPRFSNGFPLIIGGD
                     LLYEGRDAEPLLNVIERMLTPDGQLWLAEPVRRTAQRFLDSAVEHGWDIESRQVKAHW
                     PDATDGPVNLHFLRRSREPDRIATDLGGWRI"
     gene            748..>2115
                     /locus_tag="VMZ33_04085"
     CDS             748..>2115
                     /locus_tag="VMZ33_04085"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HUR16437.1"
                     /translation="MTTPAPADTIRVLDDWFERTADERLADYLALLRLPSIGTLSEHA
                     ADVRATAEFIAQRFRSWGLENVEVSPTAGHPIVYADWLHAEGAPTVLVYAHYDVQPVD
                     PLDLWQRPPFDPVVENGRVYARGSADDKAHVHMHLWSARAWLENHGHLPVNLKFVFEG
                     EEESGSDNFDPWIVANQERLAADFAVISDTGFYEGNRPAITIGLRGLVYMQIDVTGSQ
                     IDLHSGTFGGNVQNPAIALAQIIARLKNEDGSVAVPGFYDETREISEREHEEFARMPF
                     DPDKFRSDYGLNESFGEPQFLPLERRGARPTLDVNGIWGGFQGEGSKTIIPAHAHAKV
                     SCRLVADMETSRTYERVRDYVAQIAPRGVDVVVRKLNEGNWSLTPMDHPATAAAAECL
                     EEVFGEKPFYLREGGSIPAGATFTRVLGLPVVLLGFIQPDDNAHAPNENMRLDNVEGG
                     LRTIVR"
BASE COUNT          372 a          699 c          705 g          339 t
ORIGIN      
        1 ccgaaagctc gcccggtgga tcaatcggac ggtcgatctc cagcgcctga cggcagtcct
       61 tccgatatcg aagcgcgagt acacggtatt ccgccccgat gacgtttctc gcgagagcct
      121 gtttgaagcg ggtcgcgccg atcccgacaa gcagatgcct tactgggcca aggtctggcc
      181 gagcggcgtg gcgcttgccg atgtcgttgt cgagcgcagg gaagagatca ccggccggca
      241 gattctggag ctcggcgccg ggctgggcgt caccgcgagc gcggtgctgg agtacggcgg
      301 cgacctcgtg acggccgact actcggcact gccgctggca cactgccgcc tcaccacgtt
      361 ggtaaacacc ggccgcgcgc ccgaggcaac gtgcttcaac tggcgacatg acgccgagat
      421 ccgtgcggtg atggcgctcc cacgcttcag caacgggttt ccgctgatca tcggcggcga
      481 cctgctctac gagggccgag acgccgagcc actgctcaac gtgatcgagc ggatgctgac
      541 ccctgacggg cagctgtggc tggccgaacc cgtgcgaagg accgcccaac ggttcctcga
      601 ttcggccgtg gagcacggct gggatatcga gagtcgccag gtgaaggccc actggccgga
      661 cgcaaccgac ggtccggtta acctccactt tctacgaaga agccgtgagc cggaccgaat
      721 tgccacggat ctaggaggat ggcgaatatg acgactccgg ctccggccga cacgatccgc
      781 gtgctcgacg attggttcga gcgcacggct gacgagcggc tggccgatta cctcgcgctg
      841 ctgcggctgc cgagcatcgg cacgctgtcc gagcacgcgg cggacgtccg cgccacggca
      901 gagttcatcg cccagcggtt ccgcagctgg ggcctcgaga acgtcgaggt gtcgcccacc
      961 gcgggccacc ccatcgtcta cgcagattgg ctgcacgccg agggtgcgcc gaccgtgctc
     1021 gtctacgccc actatgacgt ccagccggtc gaccccctcg acctgtggca gcggccgccg
     1081 ttcgacccgg tcgtcgagaa cggtcgtgtc tacgcccgtg gctcggccga cgacaaggca
     1141 cacgttcaca tgcacctctg gtcggcgcgg gcgtggctgg agaaccacgg ccacctgccg
     1201 gtcaacctca agttcgtgtt tgagggcgaa gaggaatcgg gctccgacaa cttcgacccg
     1261 tggatcgtcg cgaaccagga gcgtctggcg gccgacttcg cggtaatcag cgatacgggc
     1321 ttttacgagg gcaacaggcc cgcgatcacg atcgggctgc gcggcctcgt gtacatgcag
     1381 atcgatgtca ccgggtcgca gatcgacctt cactcgggga ccttcggcgg caacgtccag
     1441 aacccggcaa ttgccctggc gcagatcatc gccaggctca agaacgagga cggctcggtg
     1501 gccgtgccgg gcttctacga cgagacccgc gagatctccg agcgcgagca cgaggagttc
     1561 gcgcgaatgc cgttcgaccc ggacaagttc cgctccgact acgggctcaa cgaatcgttc
     1621 ggggagccgc aattcctgcc gctggagcgg cggggggcca gaccaacgct ggacgtcaac
     1681 gggatctggg gcggcttcca gggcgagggc tccaagacga tcatccccgc ccatgcccac
     1741 gccaaggtga gctgccggct ggtggccgac atggagacga gcaggactta cgagcgcgtg
     1801 cgcgactacg tggcccagat cgcgccgcgt ggcgttgacg ttgtggttcg caagctcaac
     1861 gagggcaact ggagcctgac gccgatggac catcctgcga ctgcggcggc agccgagtgt
     1921 ctcgaggagg tcttcggcga gaagccgttc tacctacgcg aaggtggctc catccctgct
     1981 ggcgcgacgt tcacacgcgt tctgggcctg cccgtcgtgc tgcttggctt catccagcct
     2041 gacgacaacg cacacgcgcc caacgagaac atgcgtctcg acaacgtcga gggcgggctg
     2101 cggacgatcg tccgc
//
LOCUS       DATLCO010000164         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1714793, whole genome shotgun sequence.
ACCESSION   DATLCO010000164 DATLCO010000000
VERSION     DATLCO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714793"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="thyX"
                     /locus_tag="VMZ33_04090"
     CDS             complement(<1..795)
                     /gene="thyX"
                     /locus_tag="VMZ33_04090"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HUR16438.1"
                     /translation="MAAESVTAPIASANPAPVESRTPRSPATRDLGMKATLLFPTREI
                     AEQYYLPLIYTACRTCYSELMPEDIFDRAKTGQVASDKQQDLIRRVIESGHGSTIEHI
                     VFTFAISGVTRTLSHQLVRHRAGVAFDQQSQRYLNYKRPSYMVPGSLQDAPREMRDRF
                     TAEMDDSLAFYGEMLEAGIPGEDARFVMPNATRTNLIMTANLRALIHMSGLRLCTMAQ
                     WEIRRLFQLIRHEVFSVSPFLGSFLAPKCVPLGYCDEDRNRDEHCPI"
     gene            complement(826..1365)
                     /locus_tag="VMZ33_04095"
     CDS             complement(826..1365)
                     /locus_tag="VMZ33_04095"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HUR16439.1"
                     /translation="MAPLRSDLIGASMWYEAFIGRNGGFEVNISAGSGDCMAGCINQR
                     TWHYAVDFEGTVTLESEEGDEVELSTPTPSEDPSNVQILLNAGPVCPVEQIPADPDCA
                     ARAVANATVTLYAADGTEVAMATTGANGMVAFEVPAGAYYVVAQQVEGYMGTPDAQAF
                     STVGGDTVGLAMSYDTGIR"
     gene            1442..2233
                     /locus_tag="VMZ33_04100"
     CDS             1442..2233
                     /locus_tag="VMZ33_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUR16440.1"
                     /translation="MGSGRGVGEGSGAGLGDEPGGELGELDGPAPPLQPIIRAATTAA
                     SNIVVRRFVFMVPRREIFVRGSPTCGILAPMVLRRFLGRDDTTDQGESSIPAQPRSGS
                     GGETETVRRIVAELEQMDPEQRRFLAGFAYILARAAHSDLTVGEDEQSLMEQIVTDIG
                     GLTESQAVLVVQIAKHQTELYGGTEDFLVTREFKGRASDEQKRKVVESCFAVVAADHQ
                     ITAQEYAEMTQIADELDLTREDLNAIRNQYRDHLVAIQRMRGLTT"
     gene            complement(2273..>2535)
                     /locus_tag="VMZ33_04105"
     CDS             complement(2273..>2535)
                     /locus_tag="VMZ33_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HUR16441.1"
                     /translation="DPRAPKLGGLMIGFAVAADILMGGPLTGAAMNPARWFGPAAVTG
                     VWDNSFVWIIGPLLGAAAAALAYRYVFLPEADLLRTPAEPTA"
BASE COUNT          457 a          779 c          878 g          421 t
ORIGIN      
        1 gatgggacag tgttcgtcgc ggtttcgatc ttcgtcgcag tagcccagtg gcacgcactt
       61 tggtgccagg aacgatccca ggaacggcga tacggagaac acctcgtggc ggatgagctg
      121 gaacagccgc cggatctccc actgggccat ggtgcacagg cggagaccgc tcatgtggat
      181 cagcgcccgt aggttcgcgg tcatgatcag gttggtccgc gtagcgttgg gcatcacgaa
      241 ccgcgcgtcc tcgccgggga tgcccgcctc aagcatctcc ccataaaacg caagggagtc
      301 gtccatctca gcggtgaacc ggtcgcgcat ctcccgtggc gcgtcctgga gggagccggg
      361 caccatgtac gacggccgct tgtagttgag gtagcgctgc gactgctggt cgaacgccac
      421 gccggcgcgg tggcggacga gctggtgcga cagggtgcgc gttacgcccg agattgcgaa
      481 tgtgaagacg atgtgctcga tcgtggagcc gtggcccgac tcgatgacgc ggcggatcag
      541 gtcctgctgc ttatcggagg ccacctgtcc ggtctttgcc cggtcgaaga tgtcctccgg
      601 catgagctcg gaatagcagg tccggcaggc ggtatagatc agcgggaggt agtactgctc
      661 ggcaatctca cgagtcggaa aaagcagcgt tgccttcatc cccaggtcac gagtcgcggg
      721 cgaccgcggg gtgcgtgatt cgaccggagc agggttcgcg gaggcgatgg gggcggtgac
      781 ggattcagca gccatctggc tgcctagatt acgcgtcggc cggggttagc gaatgccagt
      841 gtcgtagctc atggcgagcc cgactgtgtc gccgccgacc gtgctgaagg cctgggcgtc
      901 gggcgtcccc atgtagcctt cgacttgctg cgcaacaacg tagtaagcac cggcaggcac
      961 ctcgaacgcg accatgccgt tagcgcctgt ggtcgccatg gcgacctccg tgccgtctgc
     1021 ggcgtacagc gtcaccgtgg cattggcgac tgcgcgggcc gcgcaatcag ggtcggccgg
     1081 gatctgctcg accgggcaca caggtccggc gttgagcagg atctgaacgt tggatggatc
     1141 ttcggacggt gtcggcgtgg acagctccac ctcatcgcct tcttcacttt caagcgtcac
     1201 cgtgccctcg aagtcaaccg cgtaatgcca ggtgcgctga ttgatgcagc cagccatgca
     1261 gtcgccggag ccagcgctga tgttgacctc gaagccgccg ttgcggccga tgaatgcctc
     1321 gtaccacatg gatgcgccga tcagatcgga gcggagcgga gccatggttg cccatcgggg
     1381 atcggatgcg aacacgagag cggctgcctg agccggcgtc gagacgacgg ttggccccgg
     1441 cgtgggatca gggcgcggtg tcggcgaggg gagtggggcg gggcttggcg acgaacccgg
     1501 cggtgaactc ggcgagctgg acggccctgc gccgccgctg caaccgatca tcagggcggc
     1561 gacaaccgct gcgagcaaca tcgtggtacg tcggtttgtg ttcatggtcc ctcgacgcga
     1621 gatattcgtt cgtggttccc ccacgtgtgg catcctcgca cccatggtgc tcagacgatt
     1681 cctcggcagg gacgacacga cggaccaagg cgaaagctcg attcccgctc agccacgatc
     1741 cggcagcggc ggtgaaaccg agaccgtccg tcgcatcgtt gccgagctcg agcagatgga
     1801 tccggagcag cggcggttcc tggccggatt cgcgtacatc cttgcgcgag ccgcgcacag
     1861 cgacttgact gtcggcgaag acgagcagtc gctcatggag cagatcgtga ccgacatcgg
     1921 cgggctgacc gaatcgcagg ccgtgctcgt cgtacagatc gccaagcacc agacggagct
     1981 ctacggaggg acggaggact tcctcgtgac gcgcgagttc aagggccggg catccgacga
     2041 gcagaagcgc aaagtggtcg agtcgtgctt cgccgtcgtc gccgccgacc atcagatcac
     2101 ggcccaggaa tacgccgaga tgacccagat cgctgacgag ctggacctaa cgcgcgagga
     2161 cctgaacgcg atacgtaatc agtaccggga tcacctcgtg gcgatccaac gaatgcgggg
     2221 cctcaccacc tagggatcgc gccaacctca ccccttggag tcgggccaag cctcaggccg
     2281 ttggttcggc cggtgtcctc agcaggtctg cctcgggcag gaagacatag cggtaggcca
     2341 gggcggcagc ggcggcgccg agcagcgggc cgatgatcca aacgaagctg ttgtcccaga
     2401 cgccggtcac ggccgcgggg ccgaaccagc gggcggggtt catggccgcg cccgtgagcg
     2461 ggccgcccat caggatgtcg gccgcgaccg caaaaccgat catgaggcca cccaatttgg
     2521 gcgcgcgcgg gtcga
//
LOCUS       DATLCO010000165         3975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9802901, whole genome shotgun sequence.
ACCESSION   DATLCO010000165 DATLCO010000000
VERSION     DATLCO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9802901"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VMZ33_04110"
     CDS             <1..635
                     /locus_tag="VMZ33_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16442.1"
                     /translation="DGERLALLGPSGSGKSTLLRAIAGLEPLAAGHIRWDGTDLTGTP
                     VHERRFGLMFQDYVLFPHLDVARNVAFGLEVRGDDKWEASNRVAEVLALVRMSEYERR
                     LPAQLSGGEQQRVALARTLAPGPRLLMLDEPLGALDRSLRRDLLSELLEIVRHASLTS
                     LYVTHDHEEALAFGDRVAVMRAGRLEALATPAELIANPPTEFVRSFLGND"
     gene            complement(632..1654)
                     /locus_tag="VMZ33_04115"
     CDS             complement(632..1654)
                     /locus_tag="VMZ33_04115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR16443.1"
                     /translation="MKHIGFLSFGHWSPSAYSQVRTASDALLQSIELAVAAEELGADG
                     AYYRVHHFARQLASPFPLLAAVGARTSRIEIGTAVIDMRYENPYYMAEDAGAADLIAG
                     GRLQLGISRGSPEQVIDGFRHFRHLPAEGETDADMARRYAKEFLELIEGDGFAEPNPR
                     PMFPNPPGPLRLEPYSKGLRQRIWWGAATDATAVWAAELGMNLQSSTLKFDESGEPLH
                     IQQRKQIEAYHAAWKAAGHTREPRVSVSRSIFALVDDRDRNYFAHDRDDQDKIGYLDE
                     TTRAVFGRSYVGEPDELVKKLAEDEAIAAADTLLLTIPNQLGVEYNAHVIESILQYVA
                     PELGWR"
     gene            1681..2196
                     /gene="bcp"
                     /locus_tag="VMZ33_04120"
     CDS             1681..2196
                     /gene="bcp"
                     /locus_tag="VMZ33_04120"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HUR16444.1"
                     /translation="MTDQMTTPQPAVGDLAPDFTLPDDTGTPRRLAEQYGKWVILYFY
                     PTDDTPGCTTEACAFRDANDQIEERNAAVWGVSHLGVDSKAAFKAKYGLPFVLLADED
                     HAVADSYGVWVEKNNYGKKYMGIARATFLVDPDGKIARTWPKVRSEGHAEEVLAALDE
                     ERTARSELQTA"
     gene            2302..3051
                     /locus_tag="VMZ33_04125"
     CDS             2302..3051
                     /locus_tag="VMZ33_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HUR16445.1"
                     /translation="MTHPKPDESEGQKPHEERPERVLVIAAHPDDADFGVAGTISRWT
                     RAGTVARLVCCTSGDAGADDARIDPLELARRRENEQRNAAQVVGYEGVDYLHRPDGAL
                     ENDLALREQLVRIIRQFKPDAVLSMDPTVVIHERGFIQHVDHRNAALAAVDAVYPAAR
                     NAMAFPHLVLDEGLEPHTVEHLYLFFTDKPNTWVDTSDTIDVKIAALREHVSQIRKPE
                     ELEENIRGWATAQGEEIGTAAAEGFRYLNVG"
     gene            3204..>3975
                     /locus_tag="VMZ33_04130"
     CDS             3204..>3975
                     /locus_tag="VMZ33_04130"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR16446.1"
                     /translation="MAIDPNQEFLVQPVDASWLQCNIECQEACPVGTNCRGYLNLAAE
                     GRFEEGYILSREPNPVAAMCSYVCSAPCERACRRGDIDRPLAIRAMKRFLVEWHQASG
                     IPDDMPVIEPRDTAVAVVGAGPAGIAVARELATAGHQVTVFDELPYAGGTMLIGVPAF
                     RLPREAIEMDVKLVERLGVRFRYGVRIGRDITFEQLMEDYDAVAICAGAMNAVGLDIP
                     GGELAGIQYGVDYMKLANLGEPLTVGNDVVIIGGGYTAM"
BASE COUNT          711 a         1327 c         1294 g          643 t
ORIGIN      
        1 cggacggcga gcggctcgcc ctgctcgggc ccagtggatc cggcaagtcg acgttgctgc
       61 gcgcgatcgc cggcctcgag ccgctcgccg cgggccacat tcgttgggac ggtacggacc
      121 tcacgggcac gcccgttcac gaacgacggt tcggcttgat gttccaggac tacgtgttgt
      181 tcccgcacct ggacgtggca cgcaacgtcg cgttcgggct cgaggtacgc ggcgacgaca
      241 agtgggaagc cagcaatcgc gtcgccgagg tcctcgccct cgtccggatg tcggaatacg
      301 aacgccgact accggcgcag ctttccggcg gcgagcaaca gcgagttgcg cttgcgcgca
      361 cgttggcgcc gggtccgcgt ttgctgatgc tcgatgagcc gctcggcgcc ctcgaccgaa
      421 gcctccgtcg cgacctgctc agcgagctac tggagatcgt ccgccacgcg agcctgacga
      481 gcctctacgt cacccacgac cacgaggaag cgttggcctt tggcgaccgc gtggcagtga
      541 tgcgtgccgg ccgtctcgag gcgcttgcca cgccggcgga actgatcgcg aacccgccca
      601 cggagttcgt caggagcttc ctcggcaatg actagcgcca gccgagctcg ggtgcgacgt
      661 actggaggat gctctcgatg acgtgcgcgt tgtactccac gccgagctgg ttcgggatcg
      721 tcaggagcag cgtgtcggca gcggcgattg cctcgtcctc cgcaagcttc ttgacgagct
      781 cgtcgggttc cccgacgtag ctgcgcccga acaccgcgcg cgttgtctcg tcgaggtagc
      841 cgatcttgtc ctggtcatcc cgatcgtgcg cgaagtagtt cctatcccga tcgtcgacca
      901 atgcgaagat gctgcggctg accgacactc gcggctcgcg cgtgtgaccc gctgccttcc
      961 aggccgcgtg gtacgcctcg atctgcttcc gctgctggat gtggagtggc tcccccgact
     1021 catcgaactt gagcgtggag ctctgcaggt tcatgcccag ctcggcggcc cacacggcgg
     1081 tcgcgtcggt cgctgcgccc caccagatgc gctggcgcag cccttttgag tagggctcca
     1141 gccgcagtgg gccaggcggg ttcgggaaca tcggtcgcgg gttgggctcc gcaaacccat
     1201 caccctcgat caactccagg aactccttcg cgtagcgacg cgccatgtcg gcgtcggtct
     1261 caccttccgc cggaaggtgt ctgaagtggc ggaagccatc gatcacctgc tcgggcgacc
     1321 cgcggctgat gcccagctgc aggcggccac cggcgatcag gtcggcagcg ccggcgtcct
     1381 cggccatgta gtagggattt tcatagcgca tgtcgatgac tgccgtgccg atctcgatcc
     1441 tcgacgttcg tgccccgacc gcagcgagca gcggaaaggg cgacgccagc tggcgcgcga
     1501 aatggtgcac gcggtaatac gccccgtcgg cgccaagctc ctcagcggca actgcgagct
     1561 caatcgattg caacagtgcg tccgacgccg tgcgcacctg tgagtaggcc gatggcgacc
     1621 aatgaccgaa ggaaaggaac ccaatgtgct tcacccggca atcgtagtta cgctgtggga
     1681 atgaccgacc aaatgaccac gccacagcca gcagtcggcg acctcgctcc tgatttcacg
     1741 ctgccggacg acacaggcac tccccggcgg ctcgccgagc agtacgggaa gtgggtcatc
     1801 ctctacttct acccgaccga cgacacaccc ggctgcacga ctgaagcttg cgccttccgc
     1861 gacgcaaacg accagatcga agagcgcaac gcggccgtat ggggagtcag ccatctcggg
     1921 gtcgacagca aggccgcctt caaggccaaa tacgggttgc cgttcgtgct tctggccgac
     1981 gaggaccacg cggtcgcgga cagctacggc gtgtgggtag agaagaacaa ctacggcaaa
     2041 aagtacatgg gtatcgcgcg cgcgacgttc ctggttgacc cggacggcaa gatcgcgcgg
     2101 acatggccca aggtcaggtc cgaggggcac gccgaggaag tcctggcggc gctcgatgaa
     2161 gagcggacgg cgcggtctga actgcagacg gcctgaggga acgacggaca gcatgaggca
     2221 tcgcccgtgg ggggagacga gcgatgcctc ggccgaagca tacacacaag taaggcgcgt
     2281 gcaatatgag attccggact gatgacgcat ccgaaacccg atgagagcga agggcaaaag
     2341 ccccatgagg aacgtcccga acgcgtcctg gtaatcgccg ctcaccccga cgatgcggac
     2401 tttggcgtgg ccgggacgat ttctcgctgg acgcgcgccg gaacggttgc gcggctcgtg
     2461 tgctgcacca gcggtgacgc cggcgccgac gacgcgcgca tcgatccgtt ggaactcgca
     2521 cggcgtcgcg aaaacgagca gcgcaacgcc gcccaggtcg tgggctacga gggggtcgac
     2581 tacctgcacc gacccgacgg ggcgctcgag aacgacctcg cattacgtga gcaactggtg
     2641 cggatcattc gccagttcaa gcccgacgcc gtgctgtcga tggacccgac tgtcgtcatc
     2701 cacgagcgtg gcttcatcca gcacgtcgat catcgcaacg ccgcgctggc ggcggtggac
     2761 gccgtctacc cggcggcgag gaacgccatg gccttcccgc acctcgtgct tgacgagggc
     2821 ctcgagccac acacggtcga gcacctctac ctgttcttca cggacaaacc gaacacgtgg
     2881 gtcgacacga gcgacaccat cgatgtgaag atcgccgccc tgcgcgaaca cgtctcccag
     2941 atcaggaagc cggaagagct tgaggaaaac atccgcggtt gggcgactgc ccagggcgag
     3001 gaaatcggca cggccgccgc ggaagggttc cgctacctga acgtcggctg agcgtcggct
     3061 aaccaggcca ccggaagggc ggcgatgata cgatcgtgcg cgctggcggc cgcacgacca
     3121 aagcggccaa tcggccgtcc tgcgtacgcc gcaactgccc cagaatcctt gcccaaactg
     3181 cgcctttaga ggtcagaacc tacttggcca tcgaccccaa ccaggagttc ctggttcagc
     3241 ctgtcgacgc ctcctggctg caatgcaata tcgagtgcca ggaagcgtgc ccggtcggta
     3301 caaactgccg gggctacctg aaccttgctg ctgaaggccg cttcgaggag ggctacatcc
     3361 tttcgcggga gcccaatccg gtcgcggcca tgtgctcgta cgtgtgctcg gccccgtgtg
     3421 agcgcgcgtg ccgccgcggc gacatcgacc gcccgctcgc catccgcgcc atgaaacgct
     3481 tcctcgtgga gtggcatcag gcgagcggca tccccgacga catgcccgtg atcgagccca
     3541 gagacacggc agtcgccgtg gtcggcgccg gtcccgctgg catcgcggtc gcccgggagc
     3601 tggccaccgc cgggcaccag gtcaccgtct tcgacgagct cccgtacgcc ggcggcacga
     3661 tgctcatcgg ggtccccgct ttccgtctcc cgcgcgaggc gatcgagatg gacgtcaagc
     3721 tcgtcgagcg cctgggcgtt cgcttccggt acggcgtgcg catcggccgc gacatcacgt
     3781 tcgagcagtt gatggaggac tacgacgcgg ttgccatctg cgccggcgcc atgaacgcgg
     3841 tcgggctgga catccccggc ggcgagctcg cgggcattca atacggcgtc gactacatga
     3901 agctggccaa ccttggtgag ccactgaccg tcggcaacga cgtcgtcatc atcggcggcg
     3961 gctacaccgc catgg
//
LOCUS       DATLCO010000166         3703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9166572, whole genome shotgun sequence.
ACCESSION   DATLCO010000166 DATLCO010000000
VERSION     DATLCO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9166572"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VMZ33_04135"
     CDS             <1..184
                     /locus_tag="VMZ33_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16447.1"
                     /translation="QSGFEVSQRNLNDGSVEGLRHEHKPIETVQYHPEGSPGPLDALD
                     VFDRFISRVGASRPAA"
     gene            181..3495
                     /gene="carB"
                     /locus_tag="VMZ33_04140"
     CDS             181..3495
                     /gene="carB"
                     /locus_tag="VMZ33_04140"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HUR16448.1"
                     /translation="MKKPASVLIIGSGPVIIGQAAEFDYAGTQACRALRDEGVRTILL
                     NSNPATIMTDPGVADVVYLEPLTVQSVERILEREKPEGILSGLGGQTALNLCVQLARE
                     GVLERHPGTRLLGTPLAAIEMAEDRQAFRALLDRIDQPYPPSVIVEGATPLERQQRAL
                     LAMEQVGLPAVIRPAFTLGGSGGGIVGSAAEYTERVRSGLRVSPIGQVIVERYLDGWQ
                     EVEYEVMRDAADTCICVCSMENVDPLGVHTGDSIVVAPVQTLSDQTHQRLRRAALDII
                     RALGVEGGCNVQFALSPDHSEYAVIEVNPRVSRSSALASKATGYPIARVAAQIAIGHR
                     LDEIPNAITGRTVAAFEPALDYVVVKLPRFPFDKFPGADRGLGTQMKATGEVMAIDRT
                     FGAALNKALRGLEQSGAGWLAEHPDWSADLEALAGDADVGRLRRFLAPSDSRLWRLIA
                     LLRRGVAGDVIQTATGIAPWFVSELSRLVRLEGRLRAAGPDFDDGLLIAAKRASFGDR
                     DISTLTGLDVVDVRGRRGAIGLHPGFAMVDTSAAEFAAETPYFYSTYTAAGSPPEAPP
                     VGRPASLVIGSGPVRIGQGIEFDYCAVQASEALRRLGRQSIMVNSNPETVSTDFDASS
                     RLYFESLDAESVAEVLRAETLDGEVPDAFIQFGGQTPLGLAAAVAAEGATLRGLDLDA
                     IDQTEERMRFAGLIERLGIPQPEGGMASSVDEAFVVAERVGYPVIVRPSFVIGGLAMD
                     FAYGPDDLARIIERAVAVDDERPVRIDAYLEGLELDVDAISDGVDVLIPGLMEHVERA
                     GVHSGDSIGVYPPQNIGAADQRLVVDAITRVALAIGVRGLINGQFIIRDDGVYLLEVN
                     PRASRTVPFISKVTGVPMIELATRVALGETLASMGWAAGLLEPRPLVAVKAPVFSTHK
                     LRGVDPSVGPAMQSTGEVIGIHDDPRVAMAKALLAASLRPPLATDDRPAPVALLSLAE
                     RDKRQLPELAARLVRAGYRFCATRGTATALRLHGHDVEEVARVGEAAGPTRTVVEAIG
                     SGDVTLVVNTPSPESRPVRDAGAIRMAATAEGILCLTSIDTALAAADALDPALADEIG
                     KVRPLDAWLARTAARA"
BASE COUNT          562 a         1281 c         1235 g          625 t
ORIGIN      
        1 gcagagcggt ttcgaggtca gccagcgcaa cctgaacgac ggctcggtcg agggtctgcg
       61 ccacgaacac aagccgattg agacagtcca gtaccacccc gagggttcgc ccggtccact
      121 cgacgccctc gacgtattcg accgcttcat ctcccgagtt ggcgccagca ggcctgcggc
      181 gtgaagaagc cagcctccgt cctgatcatc ggctccgggc ccgtgatcat cggccaggct
      241 gcggagttcg actacgccgg cacgcaggcc tgccgagcgc tgcgcgacga gggtgtgcgc
      301 accatcctgc tcaacagcaa cccggccacg atcatgaccg accccggcgt cgccgatgtc
      361 gtctatctcg agccactgac ggttcagtcc gtcgagcgca tcctcgagcg cgagaagccg
      421 gagggcatcc tctccggcct gggcgggcag accgcgctca acctgtgcgt gcagctggcc
      481 cgcgaaggcg tcctcgaacg acatccaggc acgagacttc tcgggacccc gctcgcggcc
      541 atcgagatgg ccgaggatcg tcaggccttc cgggcgcttc tagatcgcat cgaccagccc
      601 tatccgccgt cagtgatcgt cgagggcgcc acgccccttg agcgccagca gcgcgcacta
      661 cttgccatgg agcaggttgg gctgccagcc gttatccgac cggcattcac gttgggtggc
      721 tcaggcggcg gcatcgtcgg cagcgcggcg gagtacaccg agcgcgtccg ctctggattg
      781 cgcgtgagcc ccatcggtca ggtcatcgtc gagcgctacc tcgatggctg gcaggaggtc
      841 gagtacgagg tcatgcgcga cgccgcggac acgtgcatct gcgtgtgctc gatggagaac
      901 gtggacccgc tgggcgtcca cacgggcgac tcgatcgtcg tcgcgccggt ccagacgctc
      961 tccgaccaga cacatcagcg gttgcgtcgc gcggcactcg acatcatccg ggcactcggc
     1021 gtcgagggcg gttgcaacgt tcagttcgcg ctgtcgcccg accattcgga gtacgcggtc
     1081 atcgaggtca acccacgcgt ctcgcgatcc tcggcgctgg cttccaaggc cacgggctat
     1141 cccatcgccc gcgttgcggc ccagatcgcc atcggccacc gactcgacga gattcccaat
     1201 gcgatcaccg gccgcaccgt cgcggcgttc gagccggcac tcgactacgt cgtcgtcaag
     1261 ctgccgcgtt tcccgttcga caagttcccc ggcgcggacc gcgggcttgg cacccagatg
     1321 aaggccacgg gcgaggtgat ggcgatcgat cggacgttcg gggccgcgct taacaaggcg
     1381 ctgcgcggcc tcgagcagtc gggcgcgggt tggctggctg agcatcccga ttggtcggcc
     1441 gacctggagg cgctggctgg tgacgccgat gtcggacgtc tccggcgatt tcttgcgccc
     1501 tccgattccc gcctgtggcg gcttatcgcc ctgctgcggc gcggcgttgc gggtgacgtg
     1561 atccagacgg ccacgggcat cgcgccgtgg ttcgtgtcgg agctgtcgcg cctcgttcga
     1621 ctcgaaggac gtctccgtgc cgctgggcca gatttcgacg acgggctgct gattgccgcc
     1681 aagcgcgcat cattcggaga tcgcgacatc tctaccttga ccggcctgga tgtcgtcgat
     1741 gttcgaggtc gacgaggggc gataggcctc catccaggtt ttgcgatggt cgatacgtcg
     1801 gccgccgagt ttgccgccga aacgccctac ttctactcga cgtacaccgc cgccggttcg
     1861 ccgccggagg ctccgccagt cggccgtccg gcctcgttgg tcatcggctc ggggccggta
     1921 cgcatcgggc agggaatcga gttcgattat tgcgccgtgc aggcgtccga ggcgttgcgg
     1981 cgcctgggtc gccagtcgat catggtcaac tcaaacccgg agacggtctc aaccgacttc
     2041 gatgcctcat cacggctgta cttcgaatcg ctcgacgccg agagcgtcgc cgaggtgctc
     2101 cgggcggaga cgctggacgg agaggtgcca gatgccttca ttcagttcgg cggccagacg
     2161 ccactcggtc tcgcggccgc ggtagccgcg gagggcgcga ccttgcgcgg actggatctc
     2221 gacgcgatcg accagacaga ggaacgcatg cgcttcgccg gcctgatcga gcggctgggc
     2281 atcccccagc cggagggtgg catggcctcg tccgttgacg aggcattcgt cgtcgccgag
     2341 cgcgtcggct acccggtaat cgtgcggccg tcctttgtca tcggcggcct cgctatggac
     2401 ttcgcctacg ggcctgacga cctggcacgg atcatcgagc gggccgttgc cgtggacgat
     2461 gagcggccgg tccggatcga cgcgtacctg gagggtctgg agcttgacgt cgacgcgatc
     2521 agtgacggcg tggacgtcct catcccggga ttgatggagc acgtcgagcg cgccggcgtc
     2581 cacagtggcg actcgatcgg cgtctacccg ccacagaaca tcggcgccgc ggatcaacgg
     2641 ctcgtcgtcg acgccatcac ccgggtcgcg ctggccatcg gggtccgggg cctcatcaat
     2701 ggccagttca tcatccgcga cgacggcgtc tatctgcttg aggtcaatcc gcgcgcatca
     2761 cggacagtgc ccttcatctc caaggtcacg ggcgtgccga tgatcgagct ggccacccgc
     2821 gttgccctgg gcgagacgct ggcgtcgatg ggctgggccg cgggcctgct cgagccgcgg
     2881 ccgctcgtcg ctgtcaaggc gcctgtgttc tcgactcaca agctgcgcgg cgtcgatccg
     2941 agcgtaggcc cggcaatgca gtcaacgggc gaggtcatcg gcattcacga tgacccgcgc
     3001 gtggccatgg ccaaggcgct gctcgcggcc tcgctgcgtc cgccgctggc caccgacgat
     3061 cgcccggcgc cagttgcgct gctctccctc gccgagcgcg acaagcggca gctccccgag
     3121 ctcgcggcac gactcgtgcg tgccggctac cgcttctgcg cgacccgcgg aacggcgacc
     3181 gctctacgcc tccacgggca cgacgtcgag gaggtcgcgc gcgttggcga ggcggccggg
     3241 cctactcgca cggttgtcga agccatcggc tcaggcgacg tgacgctcgt cgtcaacacg
     3301 ccctcacctg agtcgcgccc agtccgcgac gccggagcta tccggatggc agcgacggct
     3361 gaaggaatcc tgtgcctgac ctcgatcgac accgcactcg ccgccgccga cgcactcgac
     3421 ccggcgctcg ccgacgaaat cggcaaggtt cgaccactgg acgcctggct ggctcgaacg
     3481 gcggctcgcg cctgaccgcc gccggccact tgacggcggc agctttctat aaggggtgaa
     3541 cgtcacgctg cagatcccgg atccagccct tgtcctgctc gtcggaccgt cgggcgcggg
     3601 caagtccacg ttcgcctcgc gtaacttcag cgggacggag atcctctcga gcgaccgggc
     3661 acgggagttg ctgagcgaca acccgtcgga ccagggcgcg tct
//
LOCUS       DATLCO010000167         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5448955, whole genome shotgun sequence.
ACCESSION   DATLCO010000167 DATLCO010000000
VERSION     DATLCO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5448955"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1150
                     /locus_tag="VMZ33_04145"
     CDS             218..1150
                     /locus_tag="VMZ33_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HUR16449.1"
                     /translation="MAHSQRVSAQAAVLSVLLAAVLFGTTGTAQALGPTSTTPLGVGA
                     ARLIVGGLALLLVLPVVGVRRSEAFALWRSPSGLIAGLCTALYQVSFFAGVERAGVAI
                     GTLVAIGSGPVFAGVLAGRLLGDRPSRAWIGATALCLVGLALLALAGPGTPAVDLLGI
                     ALALVAGLGYAAYTVLAKMQLGAGHAPSAVVAAAFGLGGLILIPALVTQPVAWLAQPE
                     GVALALYLGLATTTVAYLLFGRGLSVLQAGPVTTLVLAEPMVATILGTALLHEQLTIA
                     GAVGTALIVVGILVQGVVTSRGRGREGEEALLPV"
     gene            complement(1168..>2847)
                     /locus_tag="VMZ33_04150"
     CDS             complement(1168..>2847)
                     /locus_tag="VMZ33_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HUR16450.1"
                     /translation="DASITRADGRYVVSPSSDGTAVDMAQVVQLAMDAINNTSATSAQ
                     IVVEGTPVLPTVTTEQAQAAVDRAERTVAGGLTLSGEELSTTISSDILRGWVHLDEVA
                     VGQWQLSIEAGPIAQFLAAYAAETDVAPTNASFSFAGGVDQVTVVPSAAGRSLDVEAS
                     TANVLAVLQARADSQDPGSASLALFSVEPDLDTADAQALAGRITMLGTWTTNYTPQVS
                     NGQGVNIQIPTSAVDGYVVQPNEEFDFLTAIGPITSPPYVSGGVLIHGQIREDGAIGG
                     GMCSCSTTLFNAAMRAGLQIDARGNHSIYISRYPVGLDATVWMSGGQRRTMAFTNDTG
                     YPVLIKGINRPGKVVFELYGIDDGRTVELSEPRVENVVQAEAWFQYTDELPPGRREFK
                     QDRYHSFDSWVTRTVRDAAGNVIHEDTFFSHYKKLDAITLVGRYPSDPPAGTRIHPDQ
                     YRAPDDPNPTPTPDPGSLVANFTYSRDGDGTRVTFINTSSEDALSFAWTFGDGEGADN
                     KNPTHTYADFGTYRVTVTVTDADGNTATRSKRIEIAAPTTEESPSPTPPAG"
BASE COUNT          432 a          904 c          972 g          539 t
ORIGIN      
        1 caagggaacg tggatggatc tatcggtaca gtgccgatgc cggatcggta gatcgacgat
       61 aaatcatcgg acacgctcgt agtggtcttc ggctgaacgt tctggacgag gagttcgact
      121 ctcctccatc tccaccagat gtactctcaa gcgccccgct catcggttga gtgaggcgct
      181 ttttcattgc ccggaacggg cacctaccat ccggcgggtg gctcactcgc aacgtgtctc
      241 cgcgcaggcc gccgtactct ctgtgctgct cgccgcggtg ctcttcggca cgaccggcac
      301 cgcacaggcg ctgggcccca caagcacgac acctctcgga gtgggcgcgg cgcggctaat
      361 cgtcggtggg ctggcgctcc ttctcgtcct accggtcgtc ggcgtccgac gtagcgaagc
      421 gtttgcgctg tggcgctcac cttccggtct gatcgccggc ctgtgcaccg cgctgtacca
      481 ggtgagcttc ttcgccggcg tggagcgcgc aggagtcgcg atcggcaccc tggtcgcgat
      541 cggcagtggc cccgtgttcg cgggtgtact ggccggccgg ctgctcggtg accgtccgag
      601 ccgggcctgg atcggtgcca ccgcactctg cctcgtcggg ttggcgctgc tggccctcgc
      661 cggacctgga accccagccg tcgacctgct gggcatcgca ctcgctctcg tcgccggcct
      721 cggctacgcc gcctacaccg tgctggccaa gatgcagctc ggagctgggc acgccccttc
      781 agcggtggtc gcggccgcgt ttggcctcgg tggcctgatc ctcatcccgg ctctggtaac
      841 acagcccgtc gcgtggctgg cgcagcccga gggcgtagcg ctggcgttgt acctcgggct
      901 cgcgaccacg acggtggctt acctgctgtt cggacgtggg ctttcagtgc tccaggcagg
      961 cccggtgacg acgctcgtcc tggccgagcc catggtcgcg acgatcctgg gcacggccct
     1021 tctgcacgag cagctgacga tcgcgggcgc cgtgggcacg gctttgatcg tcgtcgggat
     1081 actggtccag ggtgtggtca catcgcgggg gcgtggccgc gagggggagg aggcgcttct
     1141 cccggtctaa gtgccctcta cctcagctca gccggccggc ggggtcgggc ttgggctctc
     1201 ttccgttgtc ggagccgcga tctctatccg ctttgagcga gtagccgtgt tcccgtccgc
     1261 atctgtcacc gtgaccgtca cgcgataagt accgaagtcg gcgtaggtgt gcgtcgggtt
     1321 cttgttgtcg gccccttcac cgtcgccaaa cgtccaggcg aagctcagcg cgtcctcaga
     1381 cgacgtgttg atgaaggtga cgcgagtgcc atcgccgtcg cggctgtagg tgaagttggc
     1441 gacgagcgag cccgggtccg gagttggcgt ggggttcgga tcgtcgggcg ctcgatattg
     1501 atccgggtgg atgcgggtgc ccgccggcgg atcgctcgga taccggccca cgagcgtgat
     1561 ggcgtcgagc ttcttgtagt gcgagaagaa cgtgtcctcg tggattacgt tgccagccgc
     1621 atcacgaacc gtgcgcgtga cccaggagtc gaaggagtgg tagcgatcct gcttgaactc
     1681 gcgtcggccc ggtgggagct cgtcggtgta ctggaaccac gcctccgcct gaacgacgtt
     1741 ttcaacgcgc ggctcggaca gctcgacagt tcggccatca tcgatcccgt agagctcaaa
     1801 gacgaccttt ccaggacgat taatgccctt gatcaggacc gggtagcccg tgtcgttggt
     1861 gaaggccatc gtgcggcgct ggccgccgga catccagaca gtggcgtcca aaccgaccgg
     1921 gtagcggctg atgtagatgc tgtggttgcc acgggcgtcg atctgcaggc ccgcacgcat
     1981 cgctgcattg aagagcgtcg tcgagcacga acacatgccg ccgccgatgg cgccgtcttc
     2041 gcggatctgg ccatggatga ggacgccacc cgaaacgtac ggcgggctcg tgatcggccc
     2101 gatggcggtc aggaagtcga actcctcgtt aggctggacc acataaccgt caaccgctga
     2161 cgtcgggatc tggatgttga cgccttggcc gttggaaacc tgcggcgtgt agttggttgt
     2221 ccaggtgccg agcatcgtta tgcgcccggc aagagcctgg gcgtcggccg tgtcgaggtc
     2281 gggctcaact gagaacaacg caaggctcgc ggagccgggg tcctgcgagt cggcgcgcgc
     2341 ctgaagcaca gcaagcacgt tggccgtgct ggcctcgaca tccagtgaac ggcccgcggc
     2401 gctgggcacg accgttacct ggtcgactcc cccggcgaag ctgaacgagg cgttggtggg
     2461 agcgacgtca gtttccgcgg cataagcggc caggaactgg gcgattggcc cggcctcaat
     2521 cgacagctgc cactgaccaa cggcgacctc atcaaggtgc acccagccgc gcaggatgtc
     2581 cgaggagatg gtcgtcgaaa gctcttcgcc tgagagggtg aggcccccgg caaccgtgcg
     2641 ctcggcgcgg tcaacagcgg cctgtgcctg ctctgtggtg accgtcggca ggacgggcgt
     2701 gccctcaacg acgatctggg cacttgtcgc ggaggtgttg ttgatcgcat ccattgcgag
     2761 ctgcacgacc tgcgccatat cgacggcggt gccatcagat gacggactga cgacgtagcg
     2821 gccgtccgcg cgcgtgatcg atgcatc
//
LOCUS       DATLCO010000168         3168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3102134, whole genome shotgun sequence.
ACCESSION   DATLCO010000168 DATLCO010000000
VERSION     DATLCO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3102134"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VMZ33_04155"
     CDS             complement(<1..281)
                     /locus_tag="VMZ33_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16451.1"
                     /translation="MIQVEDSERRWFRAVLILGTFVLALVLVSQVSVLLSYFSDILLV
                     LLLSWLLAFILSPLVGLILRAFPRLPRVVVVGVVYGGLFIGLSAIILVVA"
     gene            339..1334
                     /gene="trpS"
                     /locus_tag="VMZ33_04160"
     CDS             339..1334
                     /gene="trpS"
                     /locus_tag="VMZ33_04160"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HUR16452.1"
                     /translation="MRVFSGIRASGDKTLGNYSGGFRQYVATQEQAFAEGGEAFFCVV
                     DLHSITTPFEPDVLREATLSVGAWLFATGLDPERSTVFAQSHVPAHAEGAWLLGAVTA
                     YGELRRMTQFKEKSDGQDFVSAGLFNYPVLMAADILLYGADVVPIGDDQRQHLELTRN
                     IAERFNQRFGETLTVPRGVYPDEGARIKNLQEPDRLMSTTRGAPQGVVRLVDTPDEIR
                     RKFKTAVTDSGREIIHDVKGKPGISNLIEIMAVATGESIAAIQARYDGQGYGDFKKDV
                     GEAVVQLLAPFQERYRELRSDDAELRRLLALGAQKAAAAARPTLDVMYSRMGFAR"
     gene            1350..2453
                     /gene="thrC"
                     /locus_tag="VMZ33_04165"
     CDS             1350..2453
                     /gene="thrC"
                     /locus_tag="VMZ33_04165"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HUR16453.1"
                     /translation="MLRQLRQQRSSADARPRLMERYAAFLPIGGDTPNISLGEGFTPL
                     VHARNLGRAIGVPLLHLKIEGQNPTGSFKDRGMVVAVAKAVEEGAKAIVCASTGNTAA
                     SAAAYGAAVGVEVVVVLPQGQIAQGKLLQAQMAGAKVVAVDSNFDEALVVVRDMVEDT
                     AALGRKVTLVNSVNPHRLAGQKTAAFEVCEDLGGAPDYVALPVGNAGNISAYWQGFTD
                     YRDAGLVETTPHMLGFQAAGAAPLVDGKPVADPKTVATAIKIGNPASAAKALKARDES
                     GGVIEAVTDDEILAAYRDLARTEGIFCEPASAAGVAGIRKMAAAGRIDPGATIVCVLT
                     GHGLKDPDTAARSGAPVLEAQASADSVRQVLDW"
     gene            2447..>3168
                     /gene="thrB"
                     /locus_tag="VMZ33_04170"
     CDS             2447..>3168
                     /gene="thrB"
                     /locus_tag="VMZ33_04170"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HUR16454.1"
                     /translation="MVGRSSLRPRRAVPQWAKTVAVEAPASSANLGPGFDALALALDL
                     WLRVRVTPLDAGAESLVVEGEGADEIGLDGGNRFLIGLEHGLEEFALEPPIMKIEMLN
                     EIPLARGLGSSAAASVAGLRAAEALVGEEIPQARLMELATKIEGHPDNAAAALLGGFV
                     VVGSSSARFDPPGSLCVALFIPELPLRTETMRATLPEHVTHRDAAHNVSRTAMIAAAL
                     ASGLRLDLLDAMGDDRLHEPYRA"
BASE COUNT          567 a         1035 c         1070 g          496 t
ORIGIN      
        1 gcgacgacga ggatgatcgc cgacagtccg atgaacagac cgccgtagac aacgccgacg
       61 accacgacac gcggcagccg cggaaacgcc ctcaggatca gcccgacgag cggcgacagg
      121 atgaacgcca gcagccacga cagcagaagc accagcagga tgtcgctgaa gtaggacaac
      181 aggactgaga cctgcgacac cagcacgaga gcgagcacga aggtcccgag gatcaggacc
      241 gcacggaacc agcggcgctc ggagtcctcg acttggatca tgggctcatg agtctacgtc
      301 ctcgttgagg ctccgccgaa tgcgggacaa tcgcccatat gcgcgtgttc tcaggcattc
      361 gggcatcggg cgacaagacg ctcggtaact acagcggcgg gtttcgccag tacgtagcca
      421 cacaggagca ggctttcgcc gaaggcggcg aggccttctt ctgtgtcgtc gatctgcact
      481 ccatcaccac gccattcgag cctgacgtac tccgtgaagc aactctttcc gttggtgcgt
      541 ggcttttcgc caccggcctg gacccggagc gttcgactgt cttcgcgcag agtcacgtgc
      601 cggcgcacgc cgaaggggcg tggttgctcg gagccgtaac ggcctacggg gagctgcgcc
      661 ggatgaccca gttcaaggag aagtcagacg gccaggactt cgtctcggca gggctgttca
      721 actacccagt gctgatggcc gccgacatcc tgctctatgg ggctgacgtg gtgcctatcg
      781 gcgacgacca gcgccagcac ctggagctga cacgcaacat cgccgagcgc ttcaaccagc
      841 gcttcggcga gacactcacg gtgccgcgcg gcgtttatcc ggacgagggc gcgcgcatca
      901 agaacctgca ggagccggac cgtctgatgt ccacgactcg tggcgcaccg cagggtgtcg
      961 ttcggctggt cgacacacct gacgaaatcc gtcgcaagtt caagaccgcg gttaccgatt
     1021 cggggcgcga gatcatccat gacgtaaagg gaaagcccgg catctccaat ctgatcgaga
     1081 tcatggccgt ggccacgggc gagtcgatag cggccatcca ggccagatac gacggccagg
     1141 gctacggcga cttcaagaag gacgtcggcg aggccgtggt ccagctactc gcgccgttcc
     1201 aggagcgcta ccgcgagctg cgctcggacg atgccgagct gcggcggctt ctcgccctgg
     1261 gggctcagaa ggcggcagcg gccgcccggc cgacgctcga cgtcatgtac tcacgcatgg
     1321 gtttcgcccg ctagatgaag atgagcgacg tgttgcgcca gctgcggcag cagcgatcca
     1381 gcgccgacgc ccgaccgcgc ctgatggagc gctacgcggc ctttctgccg atcggcggcg
     1441 acacgccgaa catatcgctt ggtgaaggct tcacgccgct cgtccatgca cgcaacctgg
     1501 gtcgcgcgat cggcgtgcca ttgctccact tgaagatcga gggacagaat cccactggct
     1561 cgttcaagga ccgcggcatg gtcgtggccg tagcgaaggc cgtggaggag ggggcgaagg
     1621 caattgtctg cgcatcaacc ggcaacacag ctgcttcggc ggctgcgtat ggcgcggccg
     1681 tcggagtcga agtcgtcgtc gtgctgccac agggccagat cgcccagggc aagctgctcc
     1741 aggcgcagat ggccggggcc aaggtcgttg ccgtggacag caactttgac gaggcgctcg
     1801 tcgtggtgcg cgacatggtt gaggacaccg cggcgctggg ccgcaaggtg acactcgtca
     1861 actcggttaa cccgcatcga cttgccggtc agaagacggc cgcgttcgag gtctgcgagg
     1921 acctcggcgg tgcgcccgac tacgtggcac tccccgtcgg caatgccggc aacatctcgg
     1981 cctactggca gggcttcacg gactaccgcg acgccgggct ggtcgagacg acgccgcata
     2041 tgctcggctt ccaggcggcc ggcgccgcgc cgctcgtcga cggcaaaccc gtggccgacc
     2101 ccaaaacagt tgccacggcc atcaagatcg gcaaccctgc gtcggccgcg aaggcgctca
     2161 aagcccgcga tgagtcaggt ggtgtgatcg aggccgtgac cgatgacgag atcctcgctg
     2221 cctaccgcga ccttgcgcga accgagggga tcttctgcga gcccgcgtcc gcggcgggcg
     2281 tggccgggat ccgcaaaatg gcagccgcgg gtcgtatcga cccgggggcc acgatcgtgt
     2341 gtgtcctgac gggccacgga ttgaaggatc ccgacacggc cgctcgatcc ggagcgccgg
     2401 tgctggaggc acaggcatcc gcggactccg tgaggcaggt cctcgattgg tagggcgctc
     2461 gtccctccgc ccacggcgcg cggtgccaca gtgggccaag acagtcgcgg tcgaggcgcc
     2521 cgcaagcagc gccaacctgg gcccgggctt tgacgccctg gccctggccc tggacctgtg
     2581 gctgcgggtt cgcgtcacgc cactcgacgc cggcgctgag tcgctcgtag tcgaaggtga
     2641 gggcgccgac gagattggtc tcgacggcgg caatcgtttc ttgattggtc tcgagcatgg
     2701 cctcgaggag ttcgccctcg agccaccgat catgaagatc gagatgctca acgagatccc
     2761 gctggcacgt ggcctgggat cgtctgcggc ggcgagtgtc gccggtctgc gcgccgccga
     2821 ggccctggtc ggtgaggaga tcccgcaagc tcggctgatg gagctggcga ccaagattga
     2881 gggccatcca gataacgccg cggcggcgct cctggggggg tttgtggtgg tcggcagcag
     2941 ctccgcacgc ttcgatcccc cgggcagtct ttgcgtcgct ctgttcatcc cggagctccc
     3001 gctgcggacc gagaccatgc gtgcgacgct acccgagcac gtgacgcacc gagatgccgc
     3061 tcacaacgtc agccgcacgg cgatgatcgc cgccgcactc gcgagcggcc tgcgcctgga
     3121 cctgctcgac gcgatgggcg acgaccggct ccatgagccg taccgcgc
//
LOCUS       DATLCO010000169        13407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_234216, whole genome shotgun sequence.
ACCESSION   DATLCO010000169 DATLCO010000000
VERSION     DATLCO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13407
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234216"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VMZ33_04175"
     CDS             complement(<1..425)
                     /locus_tag="VMZ33_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16455.1"
                     /translation="MDEDTERLLRGTADKALDYLRSLPDRHVGAREDARTIASRIGGP
                     LPEASEDAAAVLDRMIRDVDPGIVAAAGPRYFGFVIGGTLPASLAADWMTAAWDQNTA
                     VHALSPAGAAAEQVAGEWMLDLLGLPAGWSTGLPTGAGLG"
     gene            477..1337
                     /locus_tag="VMZ33_04180"
     CDS             477..1337
                     /locus_tag="VMZ33_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HUR16456.1"
                     /translation="MPVEPPVSSPSSLGEAYALLADADGWRPVAGGTDLMVQITGEIG
                     APPERVLDIWGLHELRGISTDPTDGALVLGALTTYTEIRRSALVAGELPALAEAAATI
                     GAAQIQNRGTIGGNIVNGSPAGDTLPVLLAVDAEIVMGSASGERAVAARDFWTGYRIT
                     ARRADELVVRIRIPVPAGREVRFRKVGTRRAQAISKVVMAISWRPDGGVWRDVRVALG
                     SVAATPIRARETEGALEGAAPRRETADHAVSTITNEIKPIDDVRSTAEYRRFVTGRIL
                     HRLLRDAGGW"
     gene            1334..1942
                     /locus_tag="VMZ33_04185"
     CDS             1334..1942
                     /locus_tag="VMZ33_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HUR16457.1"
                     /translation="MKPENIRFFDTQADFRRWLAANHQIVPFQWVGFYKKGADKQAMT
                     YAEAVEEGLCFGWIDGQTNRVDDLAVTTRFSPRRPGSNWSAVNIAKVEALKAAGRMAP
                     IGLRAFEERREPKPGEYTYETRPSDLPEAYASILRRNDAAWNFYAAQRPGYRKSMSWW
                     VVSAKREDTRLRRLDALIVESEAGRIIGDLNLPKLGAKATDV"
     gene            1935..3458
                     /gene="aceB"
                     /locus_tag="VMZ33_04190"
     CDS             1935..3458
                     /gene="aceB"
                     /locus_tag="VMZ33_04190"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HUR16458.1"
                     /translation="MSEVRMVGGSVAGIDRVLTPDALEFLGRLQREFGPRHSEVLARR
                     VERQAQIDAGAQLDFLPQTAEVRAADWSVAPAPADLDDRRVEITGPADAKMTINALNS
                     GAKVFMADFEDSLSPTWENVIGGQAALQDAVRRTLTFSSVEGKSYQLGQTLATLVVRP
                     RGWHLVERHILVDGAPMSASLFDFGLYLLHNGRELLERRSGPYFYLPKMQSHLEARLW
                     NDVFIWAQDVVGVPRGSIRATVLIETIWAAFEMDEILYELREHAAGLNAGRWDYIFSI
                     IKTFSARPGWTLPDRAQVTMAVPFMRAYAQLLVNTCHRRGAHAMGGMSAFIPNRREPE
                     VTANALAKVTEDKEREAGDGFDGTWVAHPDLVPVAMEVFDRALGDHPNQKYRQRQDVS
                     VSAGQLVDFAVPGGAITEAGLATNVSVGIRYIASWLAGVGAAALDNLMEDAATTEISR
                     SQVWQWLREGEFSAAQVRAEIDKWRTHDSEAANLFETLALSEDFAEFLTLPAYARLR"
     gene            3662..4864
                     /locus_tag="VMZ33_04195"
     CDS             3662..4864
                     /locus_tag="VMZ33_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16459.1"
                     /translation="MKDREHRVGWLIGLMVVIIIAPASVAARDQIEPNDEGAPDDRLR
                     DLSASVEATGALGMYRDRRTDEMVVVVPESGSSTFDARLHDPSIRIRVETQPLERSSI
                     AAIKDRALSVATTPDAVPGGFSAFFDPRRGKVVVISSAPRDTFADLLADHPNLVEYQF
                     GRPTLHSRWSDSEPHWGGATMEFSASTCTSGFAVNNWNMNPRMVTAAHCGSLGDYATS
                     PGTGVSFGTVKVRDNYPDNDFMLVGDNSIGQQGRIYVTENSDLTHTSMGVNSANDTVE
                     GFDDYCISGSIDGGAFVSCGYEVVDETVNFCPHDQDGDWDYPGDHCNANTVLYCGTPG
                     TVYGDSGAPFYVETTSNPVRAAIRGIAIGYNDFVTGDPNLPLNCGWMERWPEISSEYN
                     VTIKTNLN"
     gene            complement(4857..5330)
                     /locus_tag="VMZ33_04200"
     CDS             complement(4857..5330)
                     /locus_tag="VMZ33_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16460.1"
                     /translation="MTYAPMLVPPDCSAPDGGWPGDIPDSIEGETALRRLADLIENQP
                     DAFSGYYRTVLNPADPESDRLTVVGATGDVKAALDELGLTYPYNLCVVPVTLDGAALE
                     LIAERLERPPARITNVRPEIGRVVLSLPYFIDTTLAELGVDARSVVVDPLVRPLN"
     gene            complement(5993..6505)
                     /locus_tag="VMZ33_04205"
     CDS             complement(5993..6505)
                     /locus_tag="VMZ33_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUR16461.1"
                     /translation="MDYISQTTAAAIAVNLDSREADAFAAWVAPHIRRMALLAERLSG
                     PFDRDDIVQEALIRAWVKRGQFDPERGSAVGWLLAITADRARRAAKRRRYTIYSTTAS
                     ETSLEDRVDVESALATLSTRQRLCVDCYYFVGLSITETAAVMSCSEGTVKSTLSDARR
                     RLKPILGEMR"
     gene            complement(6676..8055)
                     /gene="hydA"
                     /locus_tag="VMZ33_04210"
     CDS             complement(6676..8055)
                     /gene="hydA"
                     /locus_tag="VMZ33_04210"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HUR16462.1"
                     /translation="MAAELIVRGGIVVTADGRRQADVAVEGGRIVAVEHRLPESADAR
                     EIDASGLLVLPGVIDVHTHTRIATDDAPDRFFQDSVAAAFGGTTTFLSFNNPGTGIDP
                     SRSLLSDAQQWQRRTAGDSAIDYGVSAVVTSGQDDPVSELPALIDAGVATFKAFMVYD
                     FGVGDEVLRAAMRTATSRGGMLEVHCEDRGMLEANIDVLLAEGKSAPRHHAESRPPSV
                     EAAGTRRAIEIARETGATLYVVHLSCAAALDEVRAARAEGLPVYAETCPHYLTLDESR
                     YELADQDCARYVISPPLRAPADRDALWGGLADGSLALVATDHVPDKFAVEKRLTGQPF
                     TDISNGGPGIETLLSVVYNEGVARGRVTVERMVDLLSTTPARLFGLPTKGSIEVGKDA
                     DIVLFDPQERRTITQAELHHTSDYTPYEGMAVAGLVRSVLVRGRYVIRDGLFVGQRGF
                     GSFQERPLG"
     gene            complement(8040..8483)
                     /locus_tag="VMZ33_04215"
     CDS             complement(8040..8483)
                     /locus_tag="VMZ33_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR16463.1"
                     /translation="MVSRGRLMDVAGHPGFVAEADGDWLGYATYDVAGEALEVTVLES
                     LAPGRGVGSALLAACVGVAAERGVGRVWLVTTNDNLDALRFYQRRGWEMVAFHRDSIT
                     EARRTLKPEIPLLGNDGIPIRDELELELPAAQWPAFIARYAWPQS"
     gene            complement(8558..8935)
                     /locus_tag="VMZ33_04220"
     CDS             complement(8558..8935)
                     /locus_tag="VMZ33_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16464.1"
                     /translation="MNVDRVVFWGVLVVVVGVWVAVAFGMLGGGLFPIVIALAVTLVA
                     AFVGAWNLDRGRRIQTDRHFDDNAPNFYAPLENQTWQVPTSYIDHRTGGGSPPGVDDG
                     ESHEDVEGDREGRRPRGSEDPAP"
     gene            complement(8939..9364)
                     /locus_tag="VMZ33_04225"
     CDS             complement(8939..9364)
                     /locus_tag="VMZ33_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HUR16465.1"
                     /translation="MGELSIRVGDLNFTARWEADAPQTVEAIRTLLPLRSKLIHCKWS
                     GEGCWIPFGDEKLPVGYENHTSHPAPGEIIVYTGDISEGEILIAYGACDFSSKLGQLS
                     GNHFATMVEPDHQALRDRLREMGRRALWEGAQDIEITES"
     gene            9407..9727
                     /locus_tag="VMZ33_04230"
     CDS             9407..9727
                     /locus_tag="VMZ33_04230"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HUR16466.1"
                     /translation="MDEAPTISTHVLDVGGGTPGVGYHVSLYRIDGESETWAGHGTTD
                     DDGRIRRLLEGPLTAGEYRIDVEIGSVFMRRASITFAVDDPNRSYHVPFLVSAFSLTT
                     YRGT"
     gene            9724..10209
                     /locus_tag="VMZ33_04235"
     CDS             9724..10209
                     /locus_tag="VMZ33_04235"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HUR16467.1"
                     /translation="MRSTTTPNLLAALFEGAPRFVARLAQADGEPIEAVLERADDIAQ
                     SMPEEEQVELLNAHPRIGALPSTVSAMSYKEQGYDRDPGTVGLQERLDHLNEEYERRF
                     GFRFVVFVAGRPRSAIADVMESRMSASRDEELARGLSDVVAIARDRLGKVTRVEEGST
                     A"
     gene            10206..11135
                     /gene="pucL"
                     /locus_tag="VMZ33_04240"
     CDS             10206..11135
                     /gene="pucL"
                     /locus_tag="VMZ33_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HUR16468.1"
                     /translation="MSAEIYYGKADVATYRTYGTPLTGVAPIPESSFTGRNNVLMAAS
                     IEVQVLGTAFMAAYTEGDNRLVVATDTMKNFIHRESLAFNGATLEEWIAFIGTRFLET
                     YPHMERLRVMGTEIPFEPALVPGTDGEGFSASEVLFERGHGDRSTARVELERTADGGV
                     RVTEVSAGRKDIQLMKVTGSAFADFARDSYTTLPERRDRPLYVHMDMGWRYSSPDVAL
                     DPSHAGYVAGDQVADLAAAVFHGFVSLSIQHLVNEIGKVMLDRWPQLAEVSFEAQNRL
                     WDLAVTSETDERVKVYWDPHPPYGKIGLVLRRD"
     gene            complement(11122..12312)
                     /locus_tag="VMZ33_04245"
     CDS             complement(11122..12312)
                     /locus_tag="VMZ33_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HUR16469.1"
                     /translation="MTHTAGLILAAGLGRRFGGGKMLASVDGRPMLQHVLHVAAAAEL
                     DPVRVVLGDDAESVAPAMVWGEARHVRNLEPGRGLASSLLVGLAELTESDRVIVLLGD
                     QPYLTVEQVRTILAAERDPERPIVVPRYGGLPGNPVLLEREAFPLAAQLAGDRGMSQL
                     FGEHPELVRYVDIPGTNPDIDTTDDLATNHPSALVGQGYDALGDRFAQWSNGIADPMR
                     ERLLAAFFERLPKDADVVDLGCGPGLGWTADLPGKIRYTGVDLSAVQLRAAQSRVPDG
                     RFIRADMTTIDFSQASFDGVLALYSLNHLPAAAQTQMVEMIGRWLRPNGVMLATFPMS
                     GVSAWRGEWLGVPMFFGGQKIEAIRASMARADLEILVDEEVETQEPEGSVRFLWVLAK
                     LSRA"
     gene            complement(12460..>13407)
                     /locus_tag="VMZ33_04250"
     CDS             complement(12460..>13407)
                     /locus_tag="VMZ33_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HUR16470.1"
                     /translation="GAIAGSVSGGCVEGAAAELIAESRRDGLSRVVRFGISDEQAWDV
                     GLACGSTIDVLIEPQVRDELVEAARGAVGTAIITSLPDGSPDESGGRQQPASAVGAPV
                     HVLGLDGAASITPDLAAAALALAAQRHSGTVTDDGRQFFVETFAQPPRVVVFGAVQAA
                     IPLIRMAREMGYRTVVADAREAFASRERFPDADELVVGWPDEIRKHLALTPDDAVVVM
                     THDPKLDEPAIVEAVRAGCSYVGAMGSRRTQAGRRERLAAAGLSPEQIDSVRGPIGLD
                     LGGREAGEMALAILAEIVAARNDGTGRPLRDIRGSAAST"
BASE COUNT         2388 a         4384 c         4373 g         2262 t
ORIGIN      
        1 ccgagaccgg caccggtagg cagaccggtc gaccagccag ctggcagacc gagcaggtcg
       61 agcatccact cccccgcgac ctgctcggcg gcggcaccgg cgggtgacag tgcgtgcacg
      121 gcggtgttct ggtcccaggc cgcggtcatc cagtcggcgg cgagagaggc gggtagtgtc
      181 ccgccgatca cgaagccgaa gtaccgtggg ccggcagcag ccacgatccc tggatcaaca
      241 tctcgaatca tgcggtcgag aacggccgca gcgtcctccg atgcctcagg cagcgggccg
      301 ccgatgcgcg aggcgatggt gcgcgcatcc tctcgcgcgc cgacgtgccg gtccggcagc
      361 gagcgcagat agtcgagtgc cttgtcggcc gtcccccgga gcagtcgttc ggtgtcttcg
      421 tccacgacgg tagtttcgcg cttgagcgca aatcgtccgc ggcttaacct gccgtggtgc
      481 cggttgaacc tcccgtctcc tcgccgagca gtctcggtga ggcctacgcc ctgctcgccg
      541 atgcagacgg gtggcgcccc gtcgccggtg gtaccgacct gatggtgcag atcacgggcg
      601 agatcggggc gccgccggag cgcgtcctcg acatctgggg actgcacgag ctgcgtggga
      661 tttcgaccga tccgaccgat ggcgcgttgg tcctgggcgc cctcaccacc tacacggaaa
      721 ttcggcgctc agcgctggta gcgggagagc tgccggcact cgccgaggcg gccgcaacca
      781 tcggtgcggc gcagatccag aaccggggca cgatcggcgg caacatcgtg aacggctcgc
      841 ccgccgggga cacgctgccc gtgctgttgg cagtggatgc ggagattgtc atgggcagcg
      901 ctagcggcga gcgcgcggtg gccgcaaggg atttctggac gggctaccgg atcaccgccc
      961 gtcgcgccga cgagctggtc gtgcgcatcc gcatcccggt gcccgctgga cgtgaggtgc
     1021 gcttccgcaa ggtcggtacg cgacgcgccc aggcgatctc caaggtcgtg atggcgatca
     1081 gctggcggcc cgacggcggt gtgtggcgtg atgtccgcgt cgcgctgggg tcggtggccg
     1141 caacgccgat ccgcgcccgc gagaccgagg gcgccctgga gggagcggcg ccccgacgcg
     1201 agacggcgga ccatgccgtc tccacgatca cgaatgagat caagcccatc gatgatgtcc
     1261 gctccacggc cgagtaccgc cgcttcgtca ccggtcgcat cctccaccgc ctgctgcgcg
     1321 acgctggtgg ctggtgaagc cggagaacat ccggttcttc gacacgcagg ccgacttccg
     1381 gcgctggctg gccgcaaacc accagatcgt gcccttccag tgggttggct tttacaaaaa
     1441 gggggccgat aagcaggcca tgacctacgc cgaggcggtc gaggaaggcc tctgcttcgg
     1501 ctggattgac ggccagacga atcgcgttga cgacttggct gtcaccacac ggttctcgcc
     1561 acgccggccg gggagcaact ggagcgccgt gaacatcgcc aaggttgagg cgctcaaggc
     1621 cgccggacgc atggctccga tcggcttgcg ggcgttcgag gagcgccgcg agccgaaacc
     1681 gggcgagtac acctacgaga cacggccatc ggatctgccc gaggcgtacg catcgattct
     1741 gcgccgcaac gacgccgcct ggaatttcta cgcagctcag cggcccggtt atcggaagtc
     1801 aatgagctgg tgggttgttt cggccaaacg ggaggacact cgtctccgcc gccttgacgc
     1861 cctgatcgtc gagtcggagg cggggcgcat catcggcgac ctaaacctgc cgaagctggg
     1921 agcgaaggcc acggatgtct gaagttcgca tggtcggtgg atcggtcgcg gggatcgatc
     1981 gggttctcac cccagacgcg ttggaattcc tgggccgact ccagcgtgag ttcggaccgc
     2041 gccattctga ggtcctcgcg cgacgggtcg aacgccaggc gcagatcgac gccggcgccc
     2101 agctcgactt cctcccccag acggccgagg ttcgcgcggc cgattggtcg gtcgcgccgg
     2161 ctccggccga tctggacgac cgccgcgtcg agatcaccgg gccggccgac gcaaagatga
     2221 ccatcaacgc gctcaactcg ggcgcgaagg tcttcatggc cgacttcgag gactccctct
     2281 cgccgacgtg ggaaaacgtg atcgggggtc aggcggcgct tcaggacgcg gtgcggcgga
     2341 cactcacctt ctcgtctgtc gaaggaaaga gctaccagct cggccagacg ctggccacgc
     2401 tcgtcgtgcg gccgcgtggc tggcacctcg tggagcgcca catcctcgtg gacggcgcgc
     2461 cgatgtccgc gtcgctgttc gactttggcc tgtacctgct gcacaacggc agagagttgc
     2521 tcgagcgtcg atcggggccc tatttctacc tgccgaagat gcagtcgcac ctcgaggcgc
     2581 ggctctggaa cgacgtgttc atctgggcgc aggacgtcgt cggcgtgcct cggggttcga
     2641 tccgcgccac cgtcctgatt gagacgatct gggccgcctt tgagatggac gaaatcctct
     2701 acgagctgcg cgagcacgcc gcggggctca acgccggccg ttgggactac atcttcagca
     2761 tcatcaagac gttctcggcg cggcccggct ggacgctgcc cgatcgcgct caggtgacca
     2821 tggcagtgcc cttcatgcgc gcctacgcgc agctcctggt gaacacgtgc catcgccgtg
     2881 gtgcgcacgc gatgggcggc atgagcgcgt tcatccccaa ccggcgcgag ccggaggtca
     2941 ccgcaaacgc cctggccaag gtgacggagg acaaggagcg cgaggcgggc gacgggtttg
     3001 acgggacctg ggtcgcccat cccgacttgg tgcccgtggc gatggaggtg ttcgaccggg
     3061 cgctgggcga ccatccgaac cagaaatacc ggcagcgtca ggatgtgtcc gtttcggccg
     3121 ggcagctcgt ggacttcgcc gtgcccggtg gcgccatcac cgaggccggg ctggcgacca
     3181 acgtgtcggt cgggatccgc tacatcgcgt cgtggctggc gggtgtcggc gccgccgcac
     3241 tcgacaacct gatggaggac gccgccacca cggaaatcag ccgctcacag gtctggcagt
     3301 ggctacgtga gggcgagttc tcggcggcgc aggttcgcgc ggagatcgac aagtggcgga
     3361 cacacgactc tgaggccgcg aacctcttcg aaacgctggc gctgtccgaa gacttcgccg
     3421 agttcctgac actccccgcg tacgccaggc tcaggtagct gccgaaccga cgaacccggt
     3481 gcccaagtac cttacgagcc cgagtcgcac tcactcacgt caccgtgcgg ccgaccgtac
     3541 tcccgttggt tgacgacccc attgcggtca cacgcgccac gacctctggt tgacagtcgg
     3601 gactaccgtg ttcgagtcct caacgatctc agttcgtcac cgactggagg tgtgtaaggt
     3661 catgaaggat cgtgagcatc gcgtgggatg gctaattgga ttgatggtcg tcatcatcat
     3721 tgcacccgcg tccgtcgcgg ctcgagatca aatcgagccg aatgacgaag gagcaccaga
     3781 cgatcggtta cgagacctaa gcgcatcagt cgaggcaacc ggggcgcttg gcatgtatcg
     3841 tgacagacgg actgatgaga tggtcgtggt ggttccggag tccggaagta gtaccttcga
     3901 cgctcgactt catgatccgt ccattcgtat ccgagtcgaa acacaaccac tcgaacgatc
     3961 tagcatcgct gcaatcaaag accgcgctct ctccgttgcc actactcccg acgctgtccc
     4021 aggcgggttc tctgcgttct tcgatcctcg gcgtggcaag gtcgtggtca tctccagcgc
     4081 accacgcgat acttttgctg atctattagc ggaccacccg aacctcgttg aataccaatt
     4141 tggcagaccg actctgcatt cccgttggtc ggacagcgag ccgcactggg gtggtgcaac
     4201 catggagttt tcagcgtcca cttgtacgtc tggattcgcg gtcaacaatt ggaacatgaa
     4261 tccacgaatg gtaaccgccg cgcactgcgg ctccctcggc gattacgcga cgagcccggg
     4321 tactggtgtg tcgttcggaa ccgtaaaggt ccgcgacaac taccccgaca acgacttcat
     4381 gctcgtcggt gacaattcaa tcggtcaaca gggcagaatc tacgtcaccg agaattccga
     4441 tctgacgcac acgtcgatgg gggtgaatag cgcgaacgac actgtcgaag gattcgatga
     4501 ctattgcatc agcgggagca tcgacggcgg tgcattcgta agctgcggat atgaggttgt
     4561 cgatgagacg gtgaacttct gtcctcacga ccaagacggc gactgggact atcccggcga
     4621 ccactgtaac gcgaacacgg ttctttattg cgggacgcca ggaactgttt atggcgattc
     4681 gggagcgccg ttctatgttg agacgacctc aaatccagtg cgagcagcca ttagaggcat
     4741 tgcgattggg tacaatgact ttgtgaccgg agatccgaac ttgcctctca attgcggatg
     4801 gatggaacga tggcccgaga tatccagcga gtacaacgtg acgatcaaga ccaatctcaa
     4861 ttgagaggtc tgacaagcgg atcgacgacg acgcttcggg cgtccactcc gagctcggcc
     4921 agggtcgtat ctatgaagta gggcaaactg aggactactc ggccgatttc gggacgaacg
     4981 ttggtgattc gtgctggtgg acgctccagc cgctcagcga tcagctccag agcggcgccg
     5041 tcgagggtga ccggtaccac gcacaagttg tatggatagg tcagcccaag ctcatcgagg
     5101 gcagccttta catcgccagt agcgccgaca accgtcaggc gatcgctttc cggatccgcc
     5161 ggatttagaa cggtgcggta gtagccgctg aaggcgtccg gctggttctc gatcaggtcg
     5221 gccaaccgtc gtaatgctgt ctcaccttcg atggagtcag gaatatcgcc gggccacccg
     5281 ccgtcagggg cggagcaatc aggcggcacg agcatggggg cgtacgtcat cgactgtgga
     5341 tcgacttccg cgacggttcg caccacgatt gcgccgcgac gccagaaccc cgagacccga
     5401 acaagtccac tagtgccgct atccagaccg gaaacccacc cggggatgtc gtccggctgg
     5461 agaccgtcga gtcgtacgct gatttccggg gggcatccat agacgatctc ggaaggagat
     5521 gcggtggcgt tgcctaccct cagcgatccg cctgacctgc agatccgaag gtcggtgtca
     5581 ctacccctta cgaatgcgat cgcgacaact gagtcccctt cgcgtatcac ggctgctgga
     5641 ggctccaaga atgtccatgt cgtcgcatca ggacgcgttg acggagacgc cgtcggtgcc
     5701 tgtgaacccg tgccaacggc ggaaagagag gtggaccgcg gagttgctag ctttccggtc
     5761 gatggtgact gagacgaagt agtaccgaat tgtgtcgatg gtcgtgggag tgagaacaca
     5821 atcaacaaga gcatcgcgcc accgaccgcc aagccagcaa ccgcacgcac cgcccgccag
     5881 cttgacaggg gcggtgacct aagaaggctg gtatcaacaa ttggttcctc ggtcatcgca
     5941 cgagctcgcc actgttcccc ggctcgtcga aggacgtcat caacctcatc agtcatctca
     6001 tctccccaag gatcggtttc aggcgacgcc gcgcgtccga taacgtggat ttgacagttc
     6061 cctcggagca gctcatgact gctgcagtct cggtgatcga aagaccaacg aagtagtagc
     6121 agtcaacgca gaggcgttga cgcgttgaca aggtcgcgag ggcactctca acatcgacgc
     6181 ggtcctccag cgacgtctcg gaggctgtcg tggaatagat ggtgtatctc ctacgcttgg
     6241 cagcgcgtct ggctctgtcg gctgtgattg caagcagcca gcccaccgcc gacccgcgct
     6301 cagggtcaaa ctgcccgcgc tttacccagg cacgaatcag agcctcctga acgatgtcat
     6361 cccggtcgaa cggaccactc agacgctcgg cgaggagggc catgcgtcga atatgcggag
     6421 ctacccacgc ggcgaaggca tcagcctcgc ggctatccag attcaccgca atcgctgcag
     6481 cagtagtttg ggagatgtag tccacactct taatacaccc tcgcgaagac aatcggttgg
     6541 gtggacgttg catgtccccg cgcgattgca atctgcttgc gagaccgacg ctcgcccgac
     6601 ggcgtgaccg ttccagagat ggactctagc gggtggatgc tgcggcaaat acccatcgcc
     6661 tgggcgaatc gattgttagc cgagaggccg ctcctggaag ctgccgaagc cgcgctggcc
     6721 gacgaacagc ccgtcgcgga tgacgtacct gccgcggacg aggactgagc ggaccagtcc
     6781 ggccacagcc atcccctcgt acggcgtgta gtcgctcgtg tggtgcaact cggcctgcgt
     6841 gatggtgcgg cgctcctgcg gatcgaacag cacgatgtcg gcgtccttcc ccacttcaat
     6901 cgaacccttg gtgggcaggc cgaatagtcg ggcgggagtg gtcgacagca gatcgaccat
     6961 tcgctcgacg gtgacgcggc cacgggcgac gccctcgttg tagacgacgg aaagcagcgt
     7021 ctcgatgccc gggccgccgt tgcttatgtc ggtgaacggc tggccggtga gccgcttctc
     7081 gacggcgaac ttgtcgggca cgtggtccgt ggctaccaag gcgagcgagc cgtccgcaag
     7141 cccgccccag agtgcgtcgc gatccgccgg cgcgcggagc ggcggcgaga tcacgtaacg
     7201 ggcacagtcc tggtcggcga gctcgtagcg ggactcgtcg agggtcaaat agtgcgggca
     7261 cgtctcggcg tacacgggca gaccctcagc gcgagccgct cgcacctcgt ccagagccgc
     7321 cgcgcacgac aggtggacga cgtacagcgt cgcgccggtc tcacgggcga tctcgatggc
     7381 gcgccgcgta ccggcggctt ccaccgaagg cggtcgcgac tcggcgtggt gccgcggggc
     7441 tgactttccc tcggcgagca acacgtcgat gttggcctca agcatgcccc ggtcctcgca
     7501 gtgaacctcg agcatgcctc cgcgcgacgt cgcagtcctc atggcggccc gcagcacctc
     7561 gtcgccgacg ccgaagtcgt agaccatgaa tgccttgaac gtcgccacgc ccgcgtcgat
     7621 cagggccggc agctcgctca ccgggtcgtc ctggccgctc gtgacgactg cgctgacgcc
     7681 gtagtcgatc gcggaatcgc cggccgtgcg ccgctgccac tgttgagcgt cagaaagcag
     7741 cgaccgcgac ggatcgatgc ccgtgcccgg gttgttgaac gacaggaacg tggtcgtgcc
     7801 gccgaacgcg gccgcgacgg agtcctggaa gaagcggtcg ggcgcgtcgt cggtggcgat
     7861 gcgagtgtgg gtgtggacgt cgatcacgcc cgggagcacc aataggccag acgcgtcgat
     7921 ctcgcgcgca tcagccgatt ccggcagtcg atgctcgacg gccacgatcc ggccgccttc
     7981 cacggccacg tccgcctgac ggcgaccgtc cgcggtgacg acgatcccgc cgcgaacgat
     8041 cagctctgcg gccatgcgta cctcgcaatg aatgccggcc actgggcggc gggcagttcc
     8101 agctctagct cgtcacggat cgggatgccg tcgttgccga gaagcgggat ctccggcttg
     8161 agcgtccggc gcgcctccgt gatcgagtcg cgatggaaag cgaccatctc ccagccgcgc
     8221 cgctggtaga accgcagcgc gtcgaggttg tcgttggtgg tcacgagcca gacccggccg
     8281 acaccgcgct cggcagccac accgacgcat gccgcgagca acgcgctccc gaccccccgc
     8341 cccggcgcca aggactcgag caccgtcacc tccagcgcct cgccggcaac gtcgtacgtg
     8401 gcgtagccga gccaatcccc gtcagcctcg gccacgaaac ccggatgacc ggcaacgtcc
     8461 atcagccggc cgcgcgaaac catccgcgtg gatccccagc tcgcggtcag gcagtccact
     8521 agacggccac ggtcctcttc ctcgagccct cgaacgatca gggcgccgga tcctcggagc
     8581 cacgcggccg ccgtccctcg cgatcgccct ccacgtcttc gtgcgactcg ccgtcatcaa
     8641 ctcctggcgg cgatccgcca cccgtgcggt ggtcgatgta tgaggtcggc acctgccacg
     8701 tctggttctc gagaggtgcg tagaagttcg gggcgttgtc gtcgaagtgg cgatcagtct
     8761 ggatccgtcg tccgcggtcc aggttccacg ccccaacgaa cgccgccacc aacgtcacgg
     8821 ccagcgcgat cacgatggga aacaagccgc cgcccaacat cccgaacgcg acggcgaccc
     8881 acacgcccac gacaacgacc agcacgcccc agaagacgac ccgatcgacg ttcacccgct
     8941 acgactccgt gatctcgatg tcctgggcgc cctcccaaag ggcgcgtcgg cccatctcgc
     9001 gcagtcggtc gcgcagggcc tggtggtcgg gttcaaccat cgtcgcgaag tgattgcctg
     9061 acagctgacc gagcttggag gagaagtcgc aggcgccata cgcgatgagg atctcgcctt
     9121 cggagatgtc gcccgtgtag acgatgatct cccccggcgc tgggtgcgat gtgtggttct
     9181 cgtagccgac cggcagcttc tcgtcgccga acggtatcca gcaaccctcg ccgctccact
     9241 tgcagtggat gagcttgctg cgcagcggca gcagcgtgcg gatggcctcc acagtctgtg
     9301 gggcgtcggc ctcccatcgg gcagtaaagt tcaggtcgcc gacgcggatc gaaagttcgc
     9361 ccatatgcgg cgaagggtag cgcgagcaaa cgcgtagagg agtcccatgg acgaggcccc
     9421 gacgatttca acgcacgttc tcgacgtcgg cggcgggacg ccgggcgtcg gctatcacgt
     9481 gtccctgtac cggatcgacg gtgagtcgga gacgtgggcg ggccacggca ccaccgacga
     9541 tgacggccgg atccgtcgcc tgctcgaggg ccctttgacc gccggggagt accggatcga
     9601 tgtcgagatc ggctccgtat tcatgcgccg cgcttccatc accttcgccg tcgatgaccc
     9661 caaccgcagc tatcacgtcc cgttcctcgt gtcggccttt tcgctcacaa cctaccgcgg
     9721 gacgtgaggt cgaccaccac cccgaacctg ctcgcggcct tgttcgaggg tgcgccgcgc
     9781 ttcgtggcac gcctggcgca ggcggatgga gagccgatcg aggccgtcct cgaacgcgcg
     9841 gacgacatcg cccaatcgat gccggaggag gagcaggtcg agctcctgaa cgcccacccg
     9901 cggattggcg ccctgccgag cacggtgtcg gccatgtcct acaaggagca aggctacgac
     9961 cgagatcctg gcacggtggg gcttcaggag cggctcgacc acctgaacga ggagtacgag
    10021 cgccgtttcg gcttccgctt cgtggttttc gtggcaggtc gaccgcggtc ggctatcgcc
    10081 gacgtcatgg agagtcggat gtccgcttcg cgggatgagg agctggcgcg aggtctgtcg
    10141 gacgtcgtgg caatcgcaag ggaccgactg ggaaaagtca cgagggtgga ggagggttca
    10201 accgcatgag cgccgagatc tactacggca aagccgacgt cgcgacctac cgcacgtacg
    10261 gcacgcccct gaccggggtc gcgccgatcc ccgagtcaag cttcaccggg cgcaacaacg
    10321 tcctcatggc ggcgagcatc gaggtccagg tcctgggcac ggcgttcatg gcggcgtaca
    10381 cggagggcga caaccggctc gtcgtcgcca cggacacgat gaagaacttc atccatcgcg
    10441 agtcgctggc gttcaacggc gcgaccctcg aggagtggat cgcgttcatc ggcacccggt
    10501 tcctggagac gtacccgcac atggagcgcc tccgtgtcat gggtacggag attccgttcg
    10561 agccggccct cgtgccgggg accgatggcg agggcttttc ggcgagcgaa gtcctgttcg
    10621 agcgcggaca cggcgaccgg tcgactgcca gggttgagct ggagcggacg gccgatggtg
    10681 gcgttcgggt gaccgaagtt tcggccggac gcaaggacat ccagctgatg aaggtcaccg
    10741 ggtcagcatt tgctgacttc gcacgcgatt cgtacacgac gcttcctgag cgcagggatc
    10801 ggccgctgta cgtgcacatg gacatgggct ggcgctattc gtcgcccgac gtcgctttgg
    10861 atccgtcaca tgccggctac gtcgcgggcg accaggtggc cgacctggcg gctgcggttt
    10921 tccatgggtt tgtcagcctg tcgatccagc acctggtgaa cgagatcggg aaggtgatgc
    10981 tcgatcgctg gccccagctc gccgaggtgt cgttcgaggc acagaaccgg ctgtgggatc
    11041 tggcggtgac gtctgagacg gacgagcgtg tgaaggtcta ttgggatccg cacccgccct
    11101 acgggaagat tgggctcgtt ctcaggcgcg actgagcttc gccagcaccc acaagaagcg
    11161 aaccgatcct tcgggttcct gggtttcgac ctcctcgtcg accaagatct cgaggtcggc
    11221 acgagccatc gatgcgcgga tcgcctcgat cttctgacca ccaaagaaca tcggaacgcc
    11281 cagccactcg ccgcgccacg cagacacgcc actcatgggg aatgtggcca gcatcacgcc
    11341 attagggcgc aaccagcgcc cgatcatctc gaccatttga gtttgcgcgg ccgccggtag
    11401 gtggttgagg gagtacaacg ccaggacgcc gtcgaagctg gcctgcgaga agtcgatcgt
    11461 cgtcatgtcg gcacgaatga accgcccgtc gggcacgcgc gattgtgcgg ctcggagttg
    11521 aacggctgac agatccaccc ctgtgtaccg gattttcccc ggcagatccg cggtccaacc
    11581 gagacccggt ccacagccaa ggtctacgac atcggcgtcc ttcggcagcc tttcgaaaaa
    11641 agcggcgagc agtcgctcgc gcatgggatc agcgatgcca ttgctccatt gcgcaaaccg
    11701 atcgccgagg gcgtcgtagc cctggccgac gagggcactc ggatggttcg tggccaggtc
    11761 atcggtcgtg tcgatgtccg ggttcgtccc cggaatgtcc acgtagcgga ccagctcggg
    11821 gtgctcgccg aagagctggg acatcccgcg gtcgccagca agctgtgcgg cgagcgggaa
    11881 tgcctcgcgc tccaggagca cggggtttcc cggcagtccc ccgtagcgcg gcacgacgat
    11941 gggccgctcg gggtcgcgct cagcggccag gatggttcga acctgctcga cggtcagata
    12001 gggctggtcg cccagcaaga cgatcacgcg atcggactcg gtgagctcag cgagcccgac
    12061 caggagcgag ctggcgaggc cgcggccggg ttccaggttt cggacgtggc gcgcctcgcc
    12121 ccagaccatt gccggcgcca ctgactctgc atcgtcaccc aggacgaccc tgactgggtc
    12181 gagctcggcc gcggctgcaa catggaggac gtgctggagc atcggccgtc cgtcaacgct
    12241 cgcgagcatc tttcccccgc cgaaccgccg cccgaggccg gccgcgagga tcaaccctgc
    12301 cgtatgcgtc acatgactac cggtgcaggt gcggaggcgt tagaggttgg gcgtgcggga
    12361 cgctatcaac cgagggccca gctcgcagca ttgacataac tgcgcagtcg ataggagcgc
    12421 tgtcagcggt cgttatggcg cggacatccg caccggcagt catgtggatg ctgccgaccc
    12481 gcggatgtcg cgcagagggc ggcccgtgcc gtcatttcga gcggcaacga tctcggccag
    12541 gatcgccagg gccatctcgc cggcctcgcg accgcccagg tcgaggccga tcggtccgcg
    12601 cacactgtcg atctgctccg ggctcaggcc cgccgcggcg aggcgctcgc gccgcccggc
    12661 ctgcgtcctg cggctcccca tcgcccctac gtagctgcaa ccggcgcgca cggcctcaac
    12721 gatcgccggc tcgtcgagct ttggatcgtg cgtcatgacc acgaccgcat cgtcgggtgt
    12781 caatgccaag tgtttgcgga tttcgtcggg ccaaccaaca acgagctcgt cggcgtccgg
    12841 gaaccgctcg cgcgacgcga aagcctcacg ggcgtctgcg accaccgtcc gatagcccat
    12901 ctcgcgtgcc atgcgaatga gggggattgc cgcctgcacc gcaccgaaga caacgactcg
    12961 tggcggctgc gcgaacgttt cgacgaagaa ctggcgtccg tcgtcagtga ccgtcccaga
    13021 gtggcgctgc gcggccagcg ccaacgcggc cgcggcgagg tccggcgtga tgctggcagc
    13081 accgtcgagc cccaggacat gaaccggcgc gcccacggca ctggccggct gctgccgccc
    13141 accactctcg tccggcgagc catcggggag tgacgtgatg atcgccgttc caacagcccc
    13201 acgagccgcc tccacaagct cgtcgcgaac ctggggctcg atcagcacgt cgatcgtgct
    13261 gccgcacgcg agcccgacgt cccacgcctg ctcgtcgctg atcccgaagc gcacgacgcg
    13321 cgacaggcca tctcgtcgcg actctgcgat caactccgcg gccgcgccct cgacacatcc
    13381 gccgctcacc gaccctgcga tcgcgcc
//
LOCUS       DATLCO010000170        10063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_234331, whole genome shotgun sequence.
ACCESSION   DATLCO010000170 DATLCO010000000
VERSION     DATLCO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234331"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VMZ33_04255"
     CDS             complement(<1..569)
                     /locus_tag="VMZ33_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUR16471.1"
                     /translation="MTDLLVDERPISTESIHYNARLTRRVDLTESLGFFWVAYDGEPI
                     NFEPGQYLTIGVESNGKLIQRPYSVASSPREKDGGYEFYIRLVNGGLFTPLLWRLPEG
                     HGMSMRGPKGKFVLEPDDDRQHVFISSGTGIAPFVSMMKTMLIDGNVRPVVALHGASY
                     AYDLGYREALEAWVTDGSYPLTYVPTVSRV"
     gene            698..931
                     /locus_tag="VMZ33_04260"
     CDS             698..931
                     /locus_tag="VMZ33_04260"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HUR16472.1"
                     /translation="MTGRDAMSPELGRVLLVVGVVIAGVGLLAVLGFRLPFGNLPGDI
                     RVEGERGGLFIPLGTMILASIVLTIVLNLVLRR"
     gene            1073..3868
                     /gene="ppdK"
                     /locus_tag="VMZ33_04265"
     CDS             1073..3868
                     /gene="ppdK"
                     /locus_tag="VMZ33_04265"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HUR16473.1"
                     /translation="MTQTPTMPAQREHRAAKRYIYAWGDGRAEGDSSMRDLLGGKGAG
                     LAEMTKAGLPVPSGFTITTEACNDYFAAGETLPTGLWDDVLEAVRVVEGETGKGFGDS
                     SNPLLVSVRSGAKFSMPGMMDTVLNLGLNEETLQGLIQLTGNERFGWDAYRRFIQMFG
                     RIVMGVEGEKFDHALDQVKTANGAKQDTDLDAAALRDVAGRFREIVRAETGREFPADP
                     NEQLDLSIKAVFASWFGKRARDYREYNNIAHDLGTAVNVVTMVFGNMGEDSGTGVAFT
                     RDPNTGERVLYGEYLTNAQGEDVVAGIRTPQPISQMRDELPEAYMEFERIGQRLEQHY
                     KDVQDLEFTIERGRLYMLQTRSAKRTAAAAVRIAADMVDEGIISEHEALARIEPAHVD
                     QLLRDQFDPNAKTTAKRITAGLNASPGAAVGKAVFNADRAFEMAQAGEKVILVRIETS
                     PDDFHGMAAAQGVLTARGGATSHAAVVARQIGKPCVAGCASLIIDYGTKSARVNGLGF
                     SEGEWISLDGTTGEVFVGALPTTAARFEDQAELQKVLEWADRARRLQVWTNADKPEEA
                     AMARRYGAQGIGLCRTEHMFREGERLDIVRDAILVAFQATRAKEKTAGGETLSAEDSE
                     AVRRFDEAMAKLEKLQQGDFEGIFEAMDGLPVVVRLIDPPLHEFLPNHSEMLVDVTRM
                     EATGVARDNPKFIEAKDLLRAVDALKEQNPMLGLRGIRLGLMIPDLIKMQTRAILNAA
                     IARKRAGGDPHPEIMIPLVGHVNELAATRAILDVEAQAVQEAGGLEVDFKFGTMIEVP
                     RGALTADEIAPHAEFFSFGTNDLTQMAFGYSRDDAEGGFLLNYVERGILPANPFQTLD
                     IAGVGQLVRTAVEKGRGARPGLKIGVCGEHGGDPASIAFFDEVGLDYVSCSPYRVPVA
                     RLAAAQAALSGAAERDK"
     gene            complement(3968..4342)
                     /locus_tag="VMZ33_04270"
     CDS             complement(3968..4342)
                     /locus_tag="VMZ33_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HUR16474.1"
                     /translation="MMNADRTLTCADCGQEFVFTASEQQFYADRGFSDPRRCRNCRAN
                     RKAQMGGSDSGSMGGGGGYSSGGSGGYGGGGGGGGGFRERRPREMFEATCSSCGKTAQ
                     VPFRPTSGKPVFCDDCFSRRRA"
     gene            4635..5180
                     /locus_tag="VMZ33_04275"
     CDS             4635..5180
                     /locus_tag="VMZ33_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16475.1"
                     /translation="MLQPELCALVWLLTEKGVPLVVASAATSAAERVRTAFAAQLRVD
                     QPSRDTLPGGVVMAGSLEDVLRVLGGSAIPGSEVADEVRDIGIVLVTDESRVTVAHYV
                     RPVERDGAGHLQRRPPTLLGALDATTGSFDLFYWGYTDELAFRSGMSRVEFEDEHARR
                     TRLLVGAADGDTGLGPSHARH"
     gene            5188..6600
                     /locus_tag="VMZ33_04280"
     CDS             5188..6600
                     /locus_tag="VMZ33_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUR16476.1"
                     /translation="MAATSPRPATLGELREHGYRSRSVKEELRGNLLARLASEQPVFD
                     GIIGYEFSVIPAIENAILAGHDICFLGERGQAKTRLARLLVGLLDEAVPVVAGAELHD
                     DPLAPITPYARDLVATQGDATPIAWLPRDDRYTEKLATPDITIADLIGEVDPIKVAEG
                     RYLADATTIHYGLVPRANRGIFTLNELPDLAERIQVGLLNVLEERDVQVRGYTIRLPL
                     DVFVVASANPEDYTSRGRIITPLKDRLGSQIRTHYPRTIDDEVQIMLQERTPLAGEGI
                     PKLEEPPFMVELIAELTHLARRSPEVSQRSGVSVRVSIANLETVCANAVKRAVRLAEE
                     SAAPRISDLPALVASTAGKIELETLGDETPEERVVDRLLGRAVNNVFSRLVDIDALDE
                     VVEAFEEGLSIEAGETVPAADYVRWMQETHGLEPIVRSLGATGSPAQVASAVEFVLEG
                     LHLNRRLNKDRSGGGSRYRR"
     gene            6602..8671
                     /locus_tag="VMZ33_04285"
     CDS             6602..8671
                     /locus_tag="VMZ33_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16477.1"
                     /translation="MDAPGFSDRQSQLPPPSRVERGPFRYARWDGSQRLDELRADDVL
                     AELSDDLLAESDLSAALARLLNRGLRGQTGQPRVGGLNDLLRRLTVAREDLLARYDLG
                     DVLADVRREIDEIVATERRGVERRLAGDAGTKEAADAAHATDAQLAELAANMASKRQE
                     QLEALPADLGGRIRGLSDYDFLEPEARRSFESLLDRLRRQVLDAHFAGMSDALKSVTP
                     ESLAANREMVRQLNSLLQERLAGNEPDVSGFTSRYGKMFPGAQTLDDIIEQLAARMAQ
                     MQSLMSSLSAEQRQELQDTMDALLRDDRLRWDLAQLAATLDQLLPNGLGDGFNFTGSE
                     QVTLEGALAQLGRLRRLDQLSEQLGDASASALEDVDPDEISEFLDGDAAEQLRALQSL
                     ARALEEAGYAERHGNRLQLTPRGARRVGQGVLDELFAKLRRDAFGGHDRRAGGALGER
                     GEGSQPYEFGRPFELHLPRTMANALARGERSPGLRLDPRDFEIHDNEDTSRAATVLLV
                     DMSRSMLLRGCFLAAKKVAMALDMLIRTRYPHDELHIVGFAYYAREIAPSALPSLSWH
                     GYEYGTNLQHGLMLARQLLARSHAANRSVVVITDGEPTAHFEGGQVEFSYPPTRQTVR
                     QTLREVVRCTREGITINTFMLERSRALAEFVERMTRLNRGRAFFASPEHLGEYVLVDY
                     VDGRTRR"
     gene            complement(8680..8949)
                     /locus_tag="VMZ33_04290"
     CDS             complement(8680..8949)
                     /locus_tag="VMZ33_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16478.1"
                     /translation="MATIHLPRSLVILFNDPPPRRLDSSALSLLDLVDELEAGWPGMR
                     DRLCQPGPRFREHINVFVNGDRQTDMATPLEVDTVVYLIPAVAGG"
     gene            complement(8956..10038)
                     /locus_tag="VMZ33_04295"
     CDS             complement(8956..10038)
                     /locus_tag="VMZ33_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16479.1"
                     /translation="MSEKTELLIGTKKGAFILDSNGSRDAWSVRGPFCENWPIHHMIR
                     SRADGALYAAGGNAWYGPSVWRSGDDGATWTQSSAGITYGEGEEDPKITTIWNLTPSN
                     GTLYAGVEPAGLFRSDDAGQNWTHVAGLRDHSTRPEWQPGNGGLCLHSIVPHPTDKDR
                     IWVGISAVGAFETTDGGKTWDLRNKGVRADFYPGDPPEFGQCVHKMGLHPSNPEILFQ
                     QNHCGVYRSDDGGRQWTEITGDLPSDFGFPLAVHPHDPSTIYVIPMTAPDKGRHMIEG
                     HTQVWRSRDAGKSWQSLDNGLPRSDAYLGVLREAMATDRMSPAGVYFGTSTGQVFASH
                     DEGDSWHLAADFLPPIWSVEASTREA"
BASE COUNT         1756 a         3291 c         3352 g         1664 t
ORIGIN      
        1 acgcgcgaca cggtcgggac gtaagtgagc gggtagctgc catcagtaac ccaggcctcc
       61 agcgcctcgc ggtagccaag gtcgtacgcg taggaggcgc cgtgcagcgc gaccaccggt
      121 cgaacgttcc cgtcgatgag catcgtcttc atcatcgaca cgaagggcgc gatgccggta
      181 ccgctactga tgaacacgtg ctggcgatca tcatccggtt ccaacacgaa cttgcccttc
      241 ggcccgcgca tactcatccc atggccctcc ggcagccgcc agaggagggg ggtgaacagc
      301 ccgccgttca cgagccggat gtagaactcg tagccgccgt ccttctcgcg tggagagctc
      361 gcgaccgagt acggccgctg aatgagcttg ccgttcgact cgacgccgat ggtcaggtac
      421 tggcccggct cgaaattgat cggttcgccg tcatacgcga cccagaagaa gccgagagac
      481 tcggtgaggt cgacgcggcg cgtcagtcgg gcgttgtagt ggatcgattc ggtggagatg
      541 ggccgctcat ccactagaag gtctgtcatt ccatggatgg taagtcgtcc cgctgattgc
      601 tcgcagtaca tcaacggcat ttgacacgtt cggccggggc aatggaacga tggcagggag
      661 cctccggcgc ctagagtcgt cccaatgagc agtgaatgtg acagggcgcg acgcgatgtc
      721 ccctgagctg gggcgggtgc tgctcgttgt gggagtcgtc atcgccggtg ttggcctgct
      781 cgccgtcctt ggctttcgac tgcccttcgg caatctgccg ggcgacatcc gcgtggaagg
      841 cgagcgcggc ggccttttca tcccattggg aaccatgatc cttgcctcga tcgtgctgac
      901 gatcgtcctc aatctggtct tgaggcgctg aggccgaacg tttctggacg ccgtgccgat
      961 gggcgacgtt gagcggtcgg caggcacggc acgactacaa tcggccgact caaaccgacc
     1021 actcagccgg gccaaactgc caagcccgcc cagcacccga ggctcacatc cgatgacaca
     1081 gacaccgacc atgcctgcac agcgcgagca tcgcgccgcc aagcgttaca tctacgcctg
     1141 gggtgacggt cgcgccgagg gggattcctc gatgcgcgac ctgctcggtg gcaagggcgc
     1201 cggcctggcc gagatgacca aggcggggct acccgtcccg tctggcttca cgatcaccac
     1261 cgaggcctgc aacgactatt tcgcggcggg cgagactctg ccaacaggtc tgtgggacga
     1321 cgttctcgag gctgtgcgcg tagtcgaggg cgagaccggc aagggctttg gcgattcatc
     1381 aaacccgctg ctcgtcagcg tccgctccgg cgcgaagttt tcgatgccgg gaatgatgga
     1441 cacggtcctc aacctgggct tgaacgaaga gaccctgcaa ggactcatcc agctcaccgg
     1501 caacgagcgg tttggctggg atgcctaccg gcgcttcatc cagatgttcg gcagaatcgt
     1561 tatgggtgtg gagggcgaga agttcgacca cgctctcgac caggtcaaga ccgcgaatgg
     1621 ggcaaagcag gacacggacc tggatgctgc cgcgctgcgt gacgtagcgg gtcgtttccg
     1681 cgagatcgtc cgcgcggaaa ccggtcgcga atttcccgct gatcccaacg agcagctcga
     1741 cctgtcgatc aaggccgtct tcgcctcgtg gttcggcaag cgtgcccgtg actaccgcga
     1801 atacaacaac atcgcgcatg acctcgggac ggccgtgaac gtcgtgacga tggtcttcgg
     1861 caacatgggc gaggactccg ggactggcgt ggcgttcacg cgtgacccga acaccggcga
     1921 gcgcgtgctc tatggggagt acctgaccaa cgcgcaaggc gaggacgtcg ttgccgggat
     1981 ccggactccg cagccaatct cgcagatgcg ggacgagctg cctgaggcct acatggagtt
     2041 cgagcgcatc ggccaacgcc tcgagcagca ctacaaggac gtgcaggacc tcgagttcac
     2101 gatcgagcgc ggccgcctgt acatgctcca gacacggtcc gcaaagcgga cggcggccgc
     2161 ggccgtgcgc attgcggcgg acatggtcga cgagggcatc atcagcgagc acgaggcgct
     2221 ggcccggatc gagccggcgc acgtcgacca gctgctccgc gatcagttcg atcccaatgc
     2281 caagacgacc gccaagcgaa tcaccgccgg gctgaacgcg tcaccgggcg cggccgtcgg
     2341 caaggcggtc ttcaacgctg atcgcgcgtt cgagatggcg caggcaggcg agaaggtcat
     2401 cctcgtgcgt atcgagacct cgcccgatga tttccatggc atggctgcgg cccaaggcgt
     2461 cctcacggcg cgtggcgggg cgacttcgca cgcggccgtc gttgcgcgcc agatcggcaa
     2521 gccgtgcgtt gctggctgcg cgtcgctgat catcgactac ggcacgaagt cggcgcgcgt
     2581 caacggcctt gggttttcgg agggcgaatg gatctccctc gacggcacga ccggcgaggt
     2641 ctttgtaggc gccctgccga ccaccgcggc gcgcttcgag gatcaggcag agctccagaa
     2701 ggtcctcgag tgggccgatc gcgcccgccg cctgcaggtc tggacgaacg ctgacaagcc
     2761 cgaggaagcg gcgatggccc ggcgttatgg cgcgcagggc atcggcctgt gccggaccga
     2821 gcacatgttt cgcgagggag agcggcttga tatcgtccgc gacgcgatcc tcgtcgcgtt
     2881 ccaggccact cgcgccaagg aaaagacggc cggcggcgag acactttccg ccgaagactc
     2941 ggaagcggtc cgacgtttcg acgaagccat ggcgaagctc gagaagctcc agcagggcga
     3001 cttcgagggg atcttcgagg ccatggacgg cctgccggtc gtcgtccgcc tgatcgaccc
     3061 gccgctgcac gagttcctgc cgaaccacag cgagatgctg gttgacgtca cccgaatgga
     3121 agctacgggc gtcgccaggg ataatccgaa gttcatcgag gcgaaggacc tgctccgggc
     3181 cgtcgacgcg ctcaaggagc agaacccgat gctcggtcta cgtggcattc gtctcgggct
     3241 gatgatcccc gacctcatca agatgcagac tcgcgccatc ctgaacgcgg ctatagcccg
     3301 gaagcgcgcg ggcggcgacc cacaccccga aatcatgatt ccgctcgtcg gtcacgtcaa
     3361 cgagctggcc gccacgcgcg ccatccttga cgtcgaggcg caggcggtcc aggaggccgg
     3421 tggcctggaa gtcgacttca agttcggcac gatgatcgag gtcccccgcg gcgccctgac
     3481 agccgacgag atcgccccgc acgccgagtt cttcagcttc gggaccaacg atctgacgca
     3541 gatggcattc gggtacagcc gcgatgacgc cgagggcggg ttcctgctga actacgtcga
     3601 gcgcggcatc ttgcccgcca acccgttcca gaccctcgac atcgccggcg tgggccagct
     3661 ggttcgcaca gcggtagaga agggccgcgg cgcgcgtcca ggcctgaaga tcggcgtttg
     3721 cggcgagcac ggcggcgacc ccgcgtcgat cgcctttttc gacgaggtcg ggctcgatta
     3781 tgtcagctgc tcgccttacc gcgttcccgt ggcacgcctc gcggcggcgc aggcggcgct
     3841 ttcgggagcg gccgaacgag acaagtagcc gaggccgcgt cccagcagaa tccgagagcc
     3901 ccggcttacg ccggggctct cagttgaccg gtgcgggacg tgacgtccgt gacgggttcg
     3961 ctagttgtta ggcgcgacgc cggctgaagc agtcgtcgca gaaaactggc ttgccactgg
     4021 tgggccgaaa cggcacctgg gcagtcttgc cgcagctcga gcatgttgcc tcgaacatct
     4081 cgcgcggccg gcgctcgcgg aagccaccgc caccaccgcc accaccgccg tagccaccgg
     4141 agccgccgga gctgtagcca ccgccaccgc ccatcgagcc cgagtcactg ccgcccatct
     4201 gtgccttgcg attcgcgcgg cagttgcgac aacgacgagg atccgagaag ccacggtcag
     4261 cgtagaactg ctgttcgctg gccgtgaaga cgaactcctg gccgcaatcg gcacaggtga
     4321 gcgtcctatc cgcgttcatc atggaactaa aaacactcct tcttcgagcc ggatttcgcg
     4381 acttttctcg cgagatccgt gagaggagtg tcgtgtaccg gcgcctattc cgcccgtatc
     4441 gacgggcttt ccctctcgca tcgtactcga cacgagccag cgggagtgtg cgcgttctaa
     4501 ccacggtggt caataggtgt tctgcaaggc tggcgcaaac aagctgtgag gcttcactgg
     4561 ccgcccagca ccgccagacg actcgagcct agactcagct cctgtctgag cttcgaaaac
     4621 aggtcgatct aggcgtgctc cagccggagc tttgcgcgct cgtgtggctc ctgactgaga
     4681 agggcgtccc gctcgtcgtc gcctccgcgg cgacgagcgc ggctgagcgc gtgcgcaccg
     4741 cattcgccgc tcagttgcgc gtcgatcagc cgtctcgcga cacgctgccc gggggcgttg
     4801 tcatggccgg ctcactggag gacgtgttgc gcgtcctggg tgggtcggca atccccggca
     4861 gcgaggtggc ggatgaggtg cgtgacattg gcatcgtgct cgtcacggac gagtcgcgag
     4921 tcaccgtggc gcactacgtt cgtccggtcg agcgggacgg ggccggtcac ctccagcgcc
     4981 gtccgccaac gctgctcggc gcgctcgacg ccacgacggg tagcttcgac ctcttctact
     5041 ggggctacac cgatgagctg gccttccgca gcggcatgtc gcgcgtcgag ttcgaggacg
     5101 agcacgctcg acgaacgcgg ctgctggtcg gcgctgcaga tggggacacg ggcctgggcc
     5161 cgtcccatgc gcgacactga ccgactgatg gcagcgacat cgccgcggcc ggccaccctg
     5221 ggggagctga gggagcacgg ctatcgcagc cgttccgtga aggaggagct gcgcggcaac
     5281 ctgctggcga ggctcgcctc ggagcaaccg gtcttcgacg ggatcatcgg ctacgaattc
     5341 agcgtgatcc ccgcgatcga gaacgcgatc ctggcgggcc atgacatctg cttcctgggg
     5401 gagcgtggcc aggccaagac tcggctggcg cgattgcttg tcggcctgct cgacgaggcc
     5461 gtgccggtgg tcgccggtgc cgagctccac gacgatccgc tggcgccgat cacgccgtac
     5521 gcgcgcgatc ttgtagccac gcagggtgat gcgacgccga tagcgtggct accgcgggac
     5581 gaccgctaca ccgagaagct ggccacgccc gacatcacca tcgctgacct catcggtgag
     5641 gtcgacccga tcaaggtggc ggagggccgc tacctggcgg acgccacgac cattcactac
     5701 ggcctcgtcc cgcgggcaaa ccgcggcatc ttcaccttga acgagctgcc cgatctggcg
     5761 gaacggatcc aagtcggcct gctcaatgtg cttgaggagc gcgatgttca ggttcgcggc
     5821 tacaccatcc ggttgccgct cgacgtattc gtcgtggcca gtgccaaccc ggaggactac
     5881 accagccgcg gccggatcat cacgccactt aaggaccggc tcggctctca aattcggacg
     5941 cactacccgc gcacgatcga cgacgaagtc cagatcatgc ttcaggagcg cacgccgttg
     6001 gccggggagg gcattcctaa gctcgaggag ccgccgttca tggtcgagct catcgctgag
     6061 ctgacgcacc ttgcgcgtcg ctccccagag gtgagccagc gctccggtgt cagcgtccgg
     6121 gtcagcatcg ccaacctcga aacggtgtgc gcaaatgcgg tcaaacgggc tgtgcggctc
     6181 gccgaggagt cggccgcgcc gcggatcagc gacctacccg cattggtggc atcaaccgcc
     6241 ggcaagatcg agctcgagac gctcggcgac gagacgccgg aggagcgcgt cgttgaccgg
     6301 ctgcttggac gtgccgtcaa caacgtattc agcaggttgg tcgacatcga cgcgctcgac
     6361 gaggtcgtcg aggcgttcga agaaggcttg tcgatcgagg ccggcgagac ggtgcccgcg
     6421 gccgactacg tacgctggat gcaggagaca catggtctcg agcccattgt caggtcgctt
     6481 ggcgcgacgg gctcaccggc tcaggtagcg tcggcggtcg agttcgtcct cgagggtctg
     6541 catctcaacc gccgcctcaa caaggaccga tccggcggag ggtcgcgcta ccgccgctga
     6601 tatggacgca ccgggtttct cggacaggca gagccagcta ccaccgccgt cgcgggtaga
     6661 gcggggccca tttcggtacg ctcgctggga cggctcgcag cggcttgacg aactgagggc
     6721 ggacgatgtc ctggccgagc tctccgacga ccttctcgcc gaatccgatc tcagtgcggc
     6781 gctcgccagg ttgctcaacc gtgggctgcg cgggcaaacg ggccagccgc gggtcggcgg
     6841 gctcaacgac ctgctgcgtc ggctgacagt ggcgcgcgag gacctcttgg cgcgctacga
     6901 cctcggcgac gtccttgcgg acgtgcgccg cgagattgac gagatcgtgg caacggagcg
     6961 gcgtggcgtg gagcgccggc ttgcgggtga cgccggcacc aaggaggccg ccgacgccgc
     7021 tcacgccacg gatgctcagt tggccgagct ggcggccaac atggccagca agcgacagga
     7081 acagctcgaa gcactgccgg ccgatctcgg cggacggatc cgcgggctat cggactacga
     7141 cttccttgag cccgaggcgc gacgcagctt cgagtctctc ctcgacaggt tgcgtcgaca
     7201 ggtgctcgac gctcatttcg ctggcatgtc tgacgcgctg aagtcggtca cgcccgagag
     7261 cctggcggca aaccgcgaga tggtgcgcca gctgaactcg ttgctgcagg agcgtctggc
     7321 ggggaacgag cccgacgtgt cgggcttcac ctcgcgctac ggcaagatgt tcccaggggc
     7381 ccagacgctc gacgacatca tcgagcagct cgcggcgcgg atggcccaga tgcagtcgct
     7441 catgtcctcc ctgtctgcgg agcagcgcca ggagctccag gacaccatgg acgccttgct
     7501 gcgggacgat cgcctccgct gggacctggc acagctcgcg gctaccctcg atcagctgct
     7561 ccccaacgga ctgggcgacg gcttcaattt cactggatcc gagcaggtga cgctcgaggg
     7621 cgcgctggcg cagctcggac ggttgcggcg gctcgaccaa ctgtcggagc agctgggcga
     7681 tgcaagtgcg agcgcgctcg aggacgtcga ccccgacgag atcagcgagt tcctggacgg
     7741 tgacgccgcc gaacagctac gggcgctgca gtcactggcg cgcgcgctgg aggaggctgg
     7801 ttacgccgaa cgccacggca accggctgca actgacgccc cgaggcgctc gccgcgtcgg
     7861 acagggcgtt ctcgacgagc ttttcgccaa gctgcggcgc gatgccttcg gcggccacga
     7921 ccgacgggcg ggcggcgcgc tcggagagcg cggcgagggg agtcagccgt acgaatttgg
     7981 gcggcccttc gagctgcacc tgccgcggac catggcaaat gcactggccc gtggcgagcg
     8041 atcgcccgga ctgaggctgg atccgcgcga cttcgagatc cacgacaacg aggacacgag
     8101 ccgcgccgcg accgtgctgc tagttgatat gagccgctcg atgctcctgc gcggatgctt
     8161 cctcgcagcc aagaaggtgg cgatggccct cgacatgctg atccggaccc gttacccgca
     8221 cgacgagctg cacattgtcg gattcgcgta ctacgcgcgg gagatcgcgc cctcggcgct
     8281 gccgtcgctc tcgtggcatg gctacgagta cggcacgaac ctccagcacg ggttgatgct
     8341 ggcgcgccag ctgctggcac ggagccacgc cgccaaccga tcagtcgtcg tcatcacgga
     8401 cggcgaaccg acggcgcact tcgagggcgg acaagtggag ttcagctacc cgccgacgcg
     8461 ccagacggtg cggcagacgc tgcgcgaggt tgtgcgatgc acgcgcgagg gcatcaccat
     8521 caacacgttc atgctcgagc gatcacgcgc tctggctgag ttcgtcgagc ggatgacgcg
     8581 cttgaaccgc ggccgcgcct tctttgccag ccccgagcac ctgggggagt acgtgttggt
     8641 cgactacgtg gacggccgaa cccgccgcta aggccggcgt cagccgccgg cgacagccgg
     8701 gatcagataa accacggtgt caacctccag gggcgttgcc atgtccgtct gccgatcgcc
     8761 gttgacgaac acgttgatgt gttcgcgaaa gcgcggcccc ggctggcaca gccgatcgcg
     8821 catgcccggc caaccggcct ccagctcgtc gacgaggtcg agcagcgaaa gcgcagagct
     8881 gtcgaggcga cggggtggcg gatcgttgaa gaggatcacc agcgaccgcg gcaggtggat
     8941 agttgccaag accgactacg cctcgcgcgt ggacgcctcg accgaccaga tcggtggcag
     9001 gaagtcggcc gcgagatgcc agctatcacc ctcatcgtgg ctggcgaata cctgcccggt
     9061 gctggtgccg aaatacacgc ccgccggact cattcgatcg gtcgccatcg cctcacgcag
     9121 gactccaagg taggcatcgg agcgcggcag gccgttgtca agcgactgcc acgacttacc
     9181 ggcgtcgcgg cttcgccaaa cctgtgtatg accttcgatc atgtggcgcc ccttgtcggg
     9241 cgccgtcatc gggatcacgt agatggttga agggtcgtgc gggtgcacag cgagcgggaa
     9301 gccgaagtcg gacggcagat cgccggttat ctcagtccac tgcctgccgc catcgtcgga
     9361 tctatacacg ccgcagtggt tctgttggaa gaggatctcg ggattcgaag gatgcaggcc
     9421 catcttgtgg acgcattggc cgaactcggg cgggtcgccc gggtagaagt cggctcgaac
     9481 gcccttgttg cgcaggtccc aggtcttgcc accgtccgtc gtctcgaaag cgcccacggc
     9541 tgagatgccc acccaaatcc ggtccttgtc cgtcggatgg ggaacgatcg agtgcaggca
     9601 caatccgccg ttgccgggct gccattcggg gcgggtcgag tggtcacgca gtcccgcgac
     9661 atgcgtccag ttctgcccgg catcatcgct gcggaacaga cccgccggct cgacacccgc
     9721 gtacagcgtc ccgtttgagg gcgtcagatt ccagatcgtc gtgatcttgg ggtcttcctc
     9781 gccctcgccg tacgtgatgc cggcgctcga ctgcgtccac gtcgcgccgt cgtcgccgct
     9841 tcgccatacc gacggtccgt accacgcatt gccgcccgcc gcgtacagcg cgccgtccgc
     9901 acgcgaacga atcatgtgat ggatcggcca gttctcgcaa aacgggccgc gcacgctcca
     9961 agcgtcacgc gacccgttgc tgtcgaggat gaaggcaccc ttcttggtgc cgatcaagag
    10021 ttcagtcttc tcgctcaccg cgccacttta gcgacggccg aaa
//
LOCUS       DATLCO010000171         4152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4700114, whole genome shotgun sequence.
ACCESSION   DATLCO010000171 DATLCO010000000
VERSION     DATLCO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4700114"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..486
                     /gene="mscL"
                     /locus_tag="VMZ33_04300"
     CDS             73..486
                     /gene="mscL"
                     /locus_tag="VMZ33_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HUR16480.1"
                     /translation="MSEFRAFLLKTNALALAIGVIIGAALGVVVTSLVNDIIMPPIGL
                     ALGGVDFDTLQYVLKEGATPEETVAIRYGSFIQAIITFIIVALVVFWISKRFLPNEAD
                     EAPTRLCPFCREINSMEATRCKACTSEMPPETTAA"
     gene            complement(559..1233)
                     /locus_tag="VMZ33_04305"
     CDS             complement(559..1233)
                     /locus_tag="VMZ33_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HUR16481.1"
                     /translation="MSRPKISVRPRSVRGKDVSKLRRQGLLPAVVYGAGSESRPIEMD
                     AHEFELLHRTAGRNAVVDLHIDGSRPQPVLLQSIQEHPVSRKAIHVDFLAVNMEEERT
                     IDVPIQIVGESEAIDKMGGVLLHLKDTVQVRAKPDDIPSSIELDITSLVDFEHVLHAS
                     DLIIPQGVTLLTDETEPLARVQAPRVEEPEPTAEAADGEQGAEGADGETAEDSGNSGS
                     AEESDS"
     gene            1365..2063
                     /locus_tag="VMZ33_04310"
     CDS             1365..2063
                     /locus_tag="VMZ33_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16482.1"
                     /translation="MTASILIVEDEYAVARGIEYALKQEGYDVTVARNGEEGLDAATR
                     NAPDLIILDVRLPGMDGFEMLRRVRGAGNKAPVLFLTARDEEMDKVIGLELGADDYLT
                     KPFGLRELMSRIKALLRRAYGDLADAAGGRVIRHHDLLIDLERRRVQRGTRRISLTAT
                     EFEILRHLASKPGRVFTRRELLELVRDYEALDQDEKTINVHVSHLREKLEDDPADPAF
                     ILTLRGAGYAFAER"
     gene            2088..3638
                     /locus_tag="VMZ33_04315"
     CDS             2088..3638
                     /locus_tag="VMZ33_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUR16483.1"
                     /translation="MRRLIPRFATGLRARLVLAFLAVVAIALALVFATLPRLLDGYFA
                     QQSQTDLDRRAGVAKLELAREITRYQATGGDAPRPILVPTEPLSASDGLRALLGTDSR
                     FASDLAQIVQADVTITIAPDPEHTSEVAYQSQQQLPDSFGEVGQQRESISSDPGAGQI
                     EIADLFWTQSGAAPQRVVVVTLSNPFTFRAQTLQQIIEVMTVAAALAMLAAVFASVLI
                     ADRLSEPVRRLKSAATQLADGRLETRVMPRSASPEMQELAEAFNTMAERLQHSIEFIR
                     SDRDRSRDFLADVSHELRTPIAALRTFNELFSDEPGMDEVTRKEFVEQSRRQIERLDW
                     LATNLLELSKLESGLVLLDLRPDDLRAAVENAVAQQLPNAERKNISLRMELPDEPVRQ
                     RHDPQRVGQVLTNLIGNAIKFTPIDGRVDVDLEQTKDGAELRVSDSGVGIDPRELPMV
                     FDRFYRGANSHESRASGSGLGLSIVRQIVEMHNGRVAITSTPGKGTQVSVALPNDVAI
                     SSQATVRA"
     gene            3668..>4152
                     /locus_tag="VMZ33_04320"
     CDS             3668..>4152
                     /locus_tag="VMZ33_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16484.1"
                     /translation="MDDETTDSYNRYEGYDPSAGSYSPPAQTPQPWTGSSYQQPTPQH
                     WLEPLPGQERRRSPRRRGRAGLTAVMVVLVALIAGGAGSLVTYGALLYGGALNNGQQV
                     SAVPTPSPQIQPQPSPQTVILDEQSAITAAAESVSPAVVTITVSGDATDPFSLPSTGV
                     GS"
BASE COUNT          733 a         1412 c         1309 g          698 t
ORIGIN      
        1 caggaagggt acgcatgcat ctatcgttcg cccggtcggg gggtgtcgga tgaatgagga
       61 ggctcaccgc tgatgagcga attcagggcg tttctgctga agacgaacgc gctcgcgctg
      121 gcgatcggcg tcatcatcgg cgcggcgctg ggcgtggtgg tcacgtcgct ggttaacgac
      181 atcatcatgc cgcccatcgg gctggcgctc ggtggcgtcg acttcgacac gctccagtac
      241 gtgctgaagg agggtgcgac tcccgaggaa acggtggcga tccgctacgg ctcgttcatc
      301 caggcgatca tcaccttcat catcgtcgcg ctggtggtgt tctggatcag caagcgattc
      361 ctgcccaacg aggccgacga ggcgcccacg cgcctctgcc cgttctgccg cgagatcaac
      421 tcgatggagg cgacgcgctg caaggcatgc accagcgaga tgccgcccga gaccaccgct
      481 gcttgacccc tggggacgca aatcaaaagg ccccgcgagt gccgcggggc ctttcctttt
      541 gactggtggt cgagtcggtt agctgtccga ctcttctgcc gatcctgaat tgccagagtc
      601 ctcggccgtc tcgccgtcgg caccctcggc accctgctcg ccatcggccg cctcggcagt
      661 cggctccggc tcctcgacgc gcggtgcctg gacgcgagca agtggctcgg tctcgtccgt
      721 caacagcgtc acgccctgag gaatgatgag gtccgaagca tgcagcacgt gctcaaagtc
      781 gacgagcgac gtgatgtcca gctcgatgct cgaggggatg tcgtccggct tggcgcgtac
      841 ctgcacggtg tccttgaggt gcagcaggac gccacccatc ttgtcgatcg cttcggactc
      901 gccgacgatc tggatcggca cgtcgatcgt gcgctcctcc tccatgttca cggcgaggaa
      961 gtcgacatgg atcgccttcc gtgaaacggg gtgctcctga atcgactgaa gcaggaccgg
     1021 ctgcggacgg ctgccgtcga tatgcagatc cacaaccgca tttcgaccag cggtgcggtg
     1081 cagcagctca aactcgtgcg cgtccatctc gatgggtcgc gactcactgc cggccccgta
     1141 aacgacggct ggcaacaggc cctgacggcg gagcttggat acgtccttgc cgcgaaccga
     1201 gcgcgggcga acggaaatct tcggtcgact catggggagc cggatggtag cacgggctgc
     1261 cgattcagcc cgtcgggctg ccgcatgttc gattccgtga atgtttgaga gaaacgcggc
     1321 cgcattacgg ctccaggcgt caacgattgc tacgatcaag ccccatgacc gcatcgatcc
     1381 tgatcgtcga agacgagtac gccgtcgctc gcggcattga gtacgccctg aagcaggagg
     1441 gctacgacgt gaccgtcgca cgcaacggcg aggaggggct cgacgccgcg acgcgtaacg
     1501 caccggatct gatcatcctc gacgtccgct tgccaggcat ggacgggttc gagatgctgc
     1561 gccgcgttcg tggcgccggc aacaaggcgc cggtgctgtt cctcaccgcc cgcgacgagg
     1621 agatggacaa ggtcatcggc ctggagctgg gcgccgacga ttacctgacc aaaccgttcg
     1681 gcctgcgcga gctgatgagc cgcatcaagg ccttgttgcg gcgtgcatac ggcgacctcg
     1741 ccgacgccgc cggcgggcgc gtgatccgcc accacgacct gctcatcgac ctcgagcggc
     1801 gccgggtaca gcgcggcacc cgacggatca gcctcaccgc aaccgagttc gagatcctgc
     1861 gccacctcgc gtcgaagccg ggccgcgtct tcacacggcg tgaactgctc gagctggtgc
     1921 gcgactacga ggcgctcgac caggacgaga agacgatcaa tgtccacgtc agccacctgc
     1981 gcgagaagct cgaagacgac ccggccgatc cggccttcat cctgaccttg cgcggcgctg
     2041 gctacgcctt cgctgagcgc tagccggaga caccccagag cgcctcgatg cgccgattga
     2101 taccccgctt cgccaccggc cttcgagcgc gactcgtcct tgcctttctc gcggtcgtgg
     2161 cgattgccct cgccctcgtg ttcgcgaccc tgccaagact cctggacggg tacttcgcgc
     2221 agcagtcgca gacggacctc gaccgccgag cgggcgtggc aaagcttgag ctcgcccggg
     2281 agatcacccg gtatcaggcg acgggcggag acgcaccgcg cccaattctc gtcccgactg
     2341 aaccacttag cgcatccgac ggcctcaggg cgctccttgg cacggacagc aggtttgcct
     2401 ccgacctcgc gcagatcgtc caagccgacg taacgatcac gattgcgccc gaccctgagc
     2461 acacgagcga ggtcgcctat caatcacaac agcagctgcc ggacagcttc ggggaggtcg
     2521 gccagcaacg tgagtcaatc tcgtctgacc caggcgcggg ccagatagag atcgcggatc
     2581 tgttctggac ccagtccggt gcggccccgc aaagggtcgt cgtcgtgacg ctgtctaacc
     2641 cattcacgtt cagagcacag acccttcagc agatcatcga ggtcatgaca gtggccgccg
     2701 ccctggcaat gctggcggcg gttttcgcgt ccgtgctcat tgcggatcgg ctctcggaac
     2761 cggtacgccg gttgaagtcg gccgccactc agctggctga tggccggctt gaaacgcgtg
     2821 tcatgccgcg cagcgcctcg ccggagatgc aggagctcgc cgaggctttc aacaccatgg
     2881 ccgagcggct ccagcattcc atcgagttca tccggagcga ccgtgaccgc agtcgcgact
     2941 tcctggccga cgtctcgcac gagctgcgca cccccatcgc ggcgcttcga acgttcaacg
     3001 agctcttctc cgacgagccg ggcatggacg aggtcacgcg caaggagttt gtggagcaga
     3061 gccgccggca gatcgagcgg ctggattggc tggcgaccaa cctcctggag ctgtccaagc
     3121 ttgaatcggg cctggtgctg ctcgatcttc ggccggacga tctccgggca gcggttgaga
     3181 atgccgtcgc gcagcagctc cccaacgccg agcgcaagaa catcagcctg cggatggagc
     3241 tgcccgacga gccggtacgt cagcggcacg acccacagcg cgtgggccag gtgttgacca
     3301 acctcatcgg caacgccatc aagttcaccc cgatcgacgg ccgcgtcgac gtggatctcg
     3361 agcaaacgaa ggacggcgcg gagctgcgcg tgtcggactc gggtgtcggc atcgacccgc
     3421 gcgagctacc gatggtcttc gatcgtttct accgcggcgc gaactcgcac gaatcgcgcg
     3481 cctctggctc tggcctgggc ctgtcgatcg ttcgccagat cgtcgagatg cataacggcc
     3541 gggtcgcgat caccagcaca ccgggcaagg gcacgcaggt ctcggtggca ctccccaacg
     3601 acgtagccat ttcttcacag gccactgtcc gcgcctgaac gcggttcggc ctacccttcg
     3661 actagccgtg gacgacgaga cgaccgactc atacaaccga tacgagggct acgacccctc
     3721 cgcaggcagc tacagcccgc cagcccagac gccccagccc tggacgggct cgtcatacca
     3781 gcaaccgacg ccgcagcact ggctcgagcc actccccggt caggagcgcc gccgatcgcc
     3841 gcgtaggcgc ggtcgcgccg gcctgacggc ggtaatggtc gtcctggtgg cgctcatcgc
     3901 cggtggagcc ggatcactcg tgacatatgg cgcgctgctg tacggcggcg cgctgaacaa
     3961 cggccaacag gtctcagccg tcccgacacc gagtccccag attcaaccgc agccctcccc
     4021 gcagacagtc atcctcgacg agcagtcggc catcaccgct gccgccgaat ccgtgagtcc
     4081 agccgtggtc acgatcaccg tttcgggcga cgcgacagat ccgttcagcc tcccgtcgac
     4141 gggcgtcggg tc
//
LOCUS       DATLCO010000172         7638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4701190, whole genome shotgun sequence.
ACCESSION   DATLCO010000172 DATLCO010000000
VERSION     DATLCO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4701190"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VMZ33_04325"
     CDS             <1..187
                     /locus_tag="VMZ33_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16485.1"
                     /translation="LNEKHGNEANISLDDVQRAGEANGQSVEQSLRNMGMAARQVVSG
                     QGQGDGSTQQTGSNNQG"
     gene            complement(171..1433)
                     /locus_tag="VMZ33_04330"
     CDS             complement(171..1433)
                     /locus_tag="VMZ33_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16486.1"
                     /translation="MTDLDQLRRRTVGVLFAGVALGSTGHIAAVTVTSIVALELSHDR
                     TLAGVPAAFVVFGAAVGATILGAIMGRWGRRPGLATGYLVGVLGATIAIAGIVVQSFP
                     ILLAGCLLIGFGNSSNQLSRYAAADMYPASRRASAIGIVVWAATVGAVIGPNLVQLSG
                     DVAVTLGLPRLSGPYLVPMVLVSVAAVMSFVLLRPDPRELAEDHGAIDRAESAPARIG
                     RVLRRPTVAMAILALIAGQFVMVLIMTMTPLHMTDHGHDLGAVGVVISAHVFGMFALA
                     PISGRLTDRFGPLPVIYAGMTMVLGSALLSALAPPNDQLILLAALFLLGWGWNLGFVA
                     GSSMLTGAVSRQERARVQGVSDALIWSTSAIASLGSGVIVGAWGFAWLGILGAVIVAA
                     TAAVVLWGRGAVRRSTGVAEAAPTNPGC"
     gene            complement(1509..2741)
                     /locus_tag="VMZ33_04335"
     CDS             complement(1509..2741)
                     /locus_tag="VMZ33_04335"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16487.1"
                     /translation="MTRLLNVAVPTRLGRDFRWLLSSSLINNTGDGIVLAAGPLLVAS
                     QTRDPFMVSLAVMFEYLPALLFAVPAGGIADRFDRRRIVVLSNVARALVLAFVVATIV
                     TGNVNIALVLAAIFLLGTAETFADTTASTLLPIIVEKQDLGIANARMQGGFILLNQLV
                     VPPIGAFLFAAGMAIPFGVNAVAFLFGAVLISRISADVGRVTRHASGATPKGFGTDLV
                     DGLRWIAHNVPMRTLALTIFAFNVTYGAAWGVLVLYAGDRLGMDAVGFGLLTTAAAAG
                     GIVGTLSYGALERRFSLGNMMRVGLVIETATHLALALTSSATVALVIMFVFGVHAFIW
                     GTTATVVRQRSVPNELMGRVGGVYRVAVFGGMVVGVPLGGLLAGAFGITAPFWFGFVG
                     SAVLVVLLWRQFGYIAHH"
     gene            complement(2748..3263)
                     /locus_tag="VMZ33_04340"
     CDS             complement(2748..3263)
                     /locus_tag="VMZ33_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16488.1"
                     /translation="MEDQQAVERYRYMLRTAPPDDIERAHAEAFAQLSPDQRRQLLQG
                     LSQQVPPTEIPTSDDPQSLARMATRAEMRQPGTMERTFGGYGGPGLGGMFFSTLAGAF
                     IGTAIAGAFLSDHGDGASGDANAGDQGAESPGDYTGDTGDSGGIADSGDSGGDFGGGD
                     FGDFGGGDIGF"
     gene            3366..4928
                     /locus_tag="VMZ33_04345"
     CDS             3366..4928
                     /locus_tag="VMZ33_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HUR16489.1"
                     /translation="METLVGVLTVLVAVAVIYFVAGRIGVPYPALFVLGGLALAFIPG
                     FPRIGLEPELVLLVFLPPLLFIAATETPIRELRTNLVPITRLAFGLVLVTILAVAAVA
                     QLLIPGLGPAAAFTLGAILAPTDPLAATTVFRRLGVPRVVQTLVEGEALFNDATALVA
                     YRAGIAAVAGGGTLLLLDEATNFGLAVVAGIAIGLGVGWIGAYTLRRLDEPTDEVVLS
                     LVIPYAAYLPADALGFSGVLAAVSAGLVIGSRLGRVLTPRSRVLWLGVWKILGFVLNG
                     FAFVLIGMELPVLGEALSERPLTEIVTVVTAICVVIVVTRFAWVYLSSYFPNSPYQRL
                     ASENVTFAKRMRFIVGWSGLRGAVSLAAALALPADFPERDLILLITFAVILLTLVGQG
                     MTLPYLVRRANVDAPDLDGDEETLAREVAYQAGLAEIDRQRDAWSDHLPLVERIESAL
                     RDRFDHLATEDPDETAERHQERIDHEGIRLAVIAAQRSAVIDLRDNGQINDETLRSIE
                     RELDLEELRMEG"
     gene            complement(5026..>7638)
                     /locus_tag="VMZ33_04350"
     CDS             complement(5026..>7638)
                     /locus_tag="VMZ33_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HUR16490.1"
                     /translation="QIDGACGPTGQAVYDAAVVADPEIDYNDYDLDKDGVVDFFMLVF
                     VGKGGNGDSQINGEPAPYDNIWPHSSSLEYSYSQPVTGLKGFVSDDQLKSLKGVPQCF
                     KDATYTAFDACAANGGTGQNDKPVPVRVGPYNVNPESAIDQASVISHEYGHHLGLPDY
                     YSTSYTTYNDWNLMATDFSQNMTVFSKQDLGWVVPEFLQPGETRTVTNWEEIKNDTGE
                     IHWQRPDGTFYTLSSANGDQNIHNGEAFGLKLPRRKLIESSQVQAEASLPNVWYSGRG
                     NDFGCTPVGGHNLDIALPELATVPDGTPITLTFKSKWDIEWDFDYGFTMVTTNGKDYT
                     SLPSAKQYTTPSSINPNNSACQERYGNGITGTSGSYRDSTFAVDRNPASPIYKDSPFL
                     QDEYDLSAYAGQSGVVLRFSYYTDPGLDRPGWFMDDLVLKAGSQTIYSNNFTNDDDVR
                     VFPGGCQGDGGLRVADKCTNGWSLVDATSPSTLDHAYYLELRDRSGFDANGQGQNDRG
                     PITWSAGALIEYTDESRGYGNIGAMPPRQHYIDSQPQPGLDCGEALVDDPLTERNDEN
                     KFCTDAAFTFATGDRHFDDNPTGGLEPGDWVDNFSDPASDDGLWHFAYNCLSFDVTSM
                     SGTDVNVALPSNLTANVTISALAGCAPFSYTGSYPNTAPVADAQAKPTTAAVGQLITF
                     DASGSTDDITPSSQLKYEWDLNNDGTYEKLGQVVTGSYSTVGTKTAKLRVTDKSTPAK
                     SSTDTVTVTVSAPKADLTITNHTVSNSSPRQGQQVTFTATVKNIGNATAGASQTQWFL
                     YKANGEQGRQLGAVATGSIAPGASRNVTLTIGTGNLNPGTYRIRVIADLKNVVDESNG
                     MNNTIYFNITVLRR"
BASE COUNT         1285 a         2405 c         2589 g         1359 t
ORIGIN      
        1 gctcaacgag aagcacggca acgaggcgaa catcagcctt gacgatgtcc agcgcgccgg
       61 cgaggcgaac ggccagtcgg tcgagcagtc gctgcgaaac atgggcatgg ctgcgcgcca
      121 ggtcgtgtcg ggacaaggcc agggcgacgg gtcgactcag cagacaggct ctaacaacca
      181 gggttagttg gtgcagcttc ggccacaccc gtcgacctcc gaactgctcc cctcccccac
      241 agaaccacgg cggcagtggc ggccacgatg accgcgccca ggatccccaa ccacgcgaac
      301 ccccacgcgc cgacgatcac gcccgagccc agactggcga tcgccgaggt gctccagatg
      361 agcgcgtcgg acacgccctg gacccgggcg cgctcctgtc gggataccgc gccggtgagc
      421 atcgagctgc cggccacgaa acccaggttc cagccccaac ccaggaggaa aagtgcggcc
      481 aggaggatga gctggtcatt gggcggtgcc agggccgaca acaacgccga accgagcacc
      541 atcgtcatgc ccgcgtagat gaccggaagc ggtccgaacc gatcggtcag acggcctgag
      601 atcggggcca gggcaaacat cccgaacacg tgcgcggaga tgacgacccc cacggcgccc
      661 aggtcgtggc cgtggtccgt catgtgcagc ggtgtcatgg tcatgatgag gaccatcacg
      721 aactgaccgg caatcagcgc caggattgcc atagcgacgg tcgggcggcg caggacgcga
      781 ccgatgcgag ctggcgcgga ctcagcgcgg tcgattgcac catggtcctc cgcgagctcg
      841 cgcgggtcgg gccggagcag cacaaacgac atcaccgcgg ctacggagac gagcaccatc
      901 ggaacgagat agggcccgct caaccttggc aggccgagcg ttacggccac gtctccgctg
      961 agctgtacga gatttggccc gatgacggcg cccacggtcg cagcccaaac aacgattccg
     1021 atggcagagg ctcgccgact ggcggggtac atgtccgcgg cggcgtaccg cgaaagctgg
     1081 tttgagctgt ttccgaagcc gatcaggaga cagcccgcga gcagaatcgg gaaagactgc
     1141 acgacgatgc cggcgatggc gatggtcgca cccaacacgc cgacgaggta gccagtcgcc
     1201 aggccggggc gcctccccca ccggcccatg atcgcgccga gaatcgtggc gccgactgcc
     1261 gcgccgaaaa cgacgaaggc cgccgggacg ccagcaagcg tccggtcgtg gctgagctct
     1321 agcgcaacga tcgatgtcac ggtcacggcc gcgatgtgac ctgtggagcc cagcgcgact
     1381 ccggcgaaga gcacgccaac tgtgcgccgg cgtagctggt cgagatcggt catccgccgg
     1441 aggttagcgg ccttctgtgg tgagggcagg gtctctccgc ggacgatgta gtccggcgcg
     1501 ccgaaaggtc agtggtgggc gatgtagccg aactggcgcc acagaaggac gaccaggacg
     1561 gccgatccca cgaagccgaa ccagaagggc gccgtgatgc cgaacgcgcc ggcgagcagg
     1621 ccgcccagcg gcacgcccac gaccatgccg ccgaagacgg cgacgcggta gacgccgccg
     1681 acacggccca tcagctcgtt cgggacagat cgctggcgca cgacggtggc cgtcgttccc
     1741 caaatgaagg cgtggacgcc gaagacgaac atgatcacca gggcgacggt cgctgagctg
     1801 gtgagggcga gcgccaggtg tgttgctgtc tcaatgacca ggccgacccg catcatgttg
     1861 cccaggctga atcgacgctc gagtgcccca tatgagagcg tgcccacgat gccgcccgca
     1921 gcagctgccg tggtcagcaa tccgaaaccc accgcgtcca ttcccagccg atccccggcg
     1981 tagaggacga gcacccccca cgcggcgccg tacgtgacgt tgaaggcgaa gatcgtgagg
     2041 gcgagagtgc gcatgggcac gttgtgcgcg atccagcgca ggccatcgac gagatcggtg
     2101 ccgaaaccct tgggggtcgc gccggaggcg tggcgggtga ccctgccgac atcggcggag
     2161 atcctggaga tgagcaccgc tccaaagagg aacgcgaccg cgttgacgcc gaacgggatc
     2221 gccatgccgg cggcgaacag gaaggcgccg atcggcggca ccacgagctg gttcaggagg
     2281 atgaagccgc cctgcatgcg ggcgttcgcg atgccgaggt cctgcttctc aacaatgatc
     2341 gggagcagtg tgctggcggt cgtgtcggcg aatgtctccg ccgtgcccag caggaatatc
     2401 gccgccagaa cgagggcgat attgacgttg ccggtaacga tcgtggccac gacaaacgcc
     2461 agcaccagcg cgcgcgcgac attggacaga acgacgatgc ggcggcggtc gaagcgatct
     2521 gcgatgccac cggcaggtac ggcgaagagg agtgccggca ggtactcgaa catcaccgcc
     2581 agcgagacca tgaacgggtc gcgggtctgc gaagcgacga gcagtggtcc ggcggcaagg
     2641 acgatgccat cgccggtgtt gttgatcagc gatgacgaca gcagccagcg gaagtctcgt
     2701 ccgaggcgcg tgggtaccgc gacgttgaga agccgcgtca taggcggcta gaagccgatg
     2761 tcgcctccac cgaaatcgcc gaagtccccg ccgccgaaat cgccgcccga gtcgccgctg
     2821 tcggcgatgc ctcccgagtc gcctgtgtcg cccgtgtagt cgccgggcga ttccgcgccc
     2881 tgatcaccgg cattcgcgtc gcctgaagcg ccatcgccgt ggtcgctcaa gaacgctccg
     2941 gcgatcgcgg ttccaatgaa cgctccggcg agtgtcgaga agaacatccc gccgaggccc
     3001 ggtccgccgt agccaccaaa agtgcgttcc atcgtcccgg gttggcgcat ctcggcgcgc
     3061 gtagccatgc gggctagtga ctggggatcg tccgaagttg gtatctcggt cggtgggacc
     3121 tgctgcgaga gtccctggag gagttgtcgc cgttgatcgg gcgatagttg agcgaaggct
     3181 tccgcgtgcg cgcgctcgat gtcgtctggc ggggccgtcc gcagcatgta tcggtagcgt
     3241 tcgactgctt gctggtcttc catgggccga ttctaggccc ggcccgccgc gactcccacc
     3301 cgcggcctat cggttgcccg ggcgcgtacg gccggaaacg cactcattcc agtagcgttc
     3361 cgcacgtgga aacgctcgtc ggagtcctca ccgtgctcgt cgcggtcgcc gtcatctact
     3421 tcgtcgccgg ccgcattggc gtcccatacc ccgcgctgtt cgtgctcggt ggactggccc
     3481 tggctttcat tccgggattt ccccggatcg gtctcgagcc cgagctggtc ctgctcgtgt
     3541 tcctgccgcc cttgctcttc attgcagcaa ctgagacgcc gattcgcgag ctgcgcacca
     3601 acctcgtgcc gatcacgcgc cttgccttcg gcctggtgct ggtcacgatc ctggccgtag
     3661 ccgccgttgc gcagctgctc atcccgggct taggtccggc tgcggcgttc acgctcgggg
     3721 cgatcctggc tccaactgac ccgctggccg caaccacggt ctttcgccgg ctcggtgtcc
     3781 cgcgcgtcgt ccagactctc gtcgagggtg aggcactctt caacgacgcc acggcgctcg
     3841 tggcatatcg cgccggaatt gccgcggtcg ccgggggtgg gactctcctg ctgctggacg
     3901 aggccacgaa cttcggcctg gcggtggtgg cagggatcgc catcggcctt ggcgttggct
     3961 ggattggcgc ctacacgctc cgtcggctgg acgagccaac ggatgaagtc gtcctgtcct
     4021 tggtgatccc atatgccgcc tatctcccgg cggatgccct cggcttttcg ggcgtccttg
     4081 ccgcagtaag cgccgggttg gtcatcggga gccggctcgg ccgtgtcctg accccgcgaa
     4141 gccgggtgtt gtggcttggc gtctggaaga tcctcgggtt cgtgctcaac gggttcgcat
     4201 tcgtgctgat cggcatggag ttgccggtcc ttggcgaggc gttgagtgag cgaccgctca
     4261 cggagatcgt cacagtcgtt accgccatct gcgttgtgat cgtggtcaca cgcttcgcct
     4321 gggtatacct ctcgagctac ttcccgaact cgccgtacca gcgtctggcc agcgagaacg
     4381 tcacgtttgc caagcggatg cgcttcatcg tggggtggtc cggactgcgt ggggccgtct
     4441 ccctcgcggc ggccctggca ctccccgcgg atttccccga gcgcgacctg atcctgctga
     4501 tcacctttgc cgtgatcctt ctgacactgg tcggccaggg gatgaccctc ccctacctcg
     4561 tgcgccgcgc aaatgtggac gcgccggacc tcgatggcga tgaggaaacc ctcgcgcgcg
     4621 aggtggccta ccaggcaggt ctcgcggaga tcgaccgcca gcgcgatgcc tggtcggatc
     4681 acctgccgct ggttgagcga attgagtccg cgctacgcga tcgattcgac cacctcgcta
     4741 cagaggatcc ggacgagacc gcggagcgtc accaggaacg gatcgaccac gagggcatac
     4801 ggctcgccgt catcgccgct cagcgctcag cggtgattga cctgcgcgac aacggtcaga
     4861 tcaacgacga gacgctccgg tccatagagc gcgagcttga cctcgaagag ctgcggatgg
     4921 agggatagcg ccgggcggac tgcccccgcc gccacgaaac tcggatgtga agaagccccg
     4981 gccgttgggc cggggcttcc tatgtgcctc gacggagggg gtggcctagc gtctcagcac
     5041 ggtgatgttg aagtagatcg tgttgttcat cccgttggac tcgtcgacca cgttcttgag
     5101 gtcggcgatc accctgatcc ggtacgtgcc cgggttgagg ttgccggtgc caattgtcag
     5161 cgtcacgttg cgtgatgcac ctggtgcgat ggatcccgtc gcgaccgcgc cgagctggcg
     5221 accctgctcg ccgttcgcct tgtaaaggaa ccactgcgtc tggctcgcgc ccgccgtggc
     5281 gttgccgatg ttcttcaccg tcgcggtgaa ggtcacctgc tggccctgtc gcggcgaaga
     5341 gttgctgacc gtgtgattgg tgatcgtcag gtcggccttg ggcgccgaga ccgtcaccgt
     5401 cacggtgtcg gtcgacgact tggccggcgt ggacttgtcg gtcacgcgca gcttggcggt
     5461 cttggttccg accgtcgagt acgacccggt cacgacctgg cccagcttct cgtatgtgcc
     5521 gtcgttgttg aggtcccatt cgtacttgag ctggctggac ggcgtgatgt cgtccgttga
     5581 cccactcgcg tcgaaggtga tcagctgccc gaccgccgcc gtggtcggct tggcctgagc
     5641 gtcggccacc ggcgcggtat tggggtagct gcccgtgtag ctgaacggag cgcatccggc
     5701 gagagccgag atggtcacgt ttgcggtcag gttggagggc agcgccacgt tgacgtcggt
     5761 gccgctcatc gacgtcacgt caaacgaaag gcagttgtag gcgaagtgcc acaggccgtc
     5821 gtctgacgca gggtcgctga agttgtcgac ccagtcacca ggttcgaggc cacctgtcgg
     5881 gttgtcgtcg aagtgccggt cgccggtggc gaacgtgaac gccgcgtcgg tgcagaactt
     5941 gttctcgtcg tttcgctcgg tcaacgggtc gtccaccagc gcctcgccgc agtcgagtcc
     6001 gggctgtggc tgcgagtcga tgtagtgctg gcgtggcggc atcgcgccga tgttgccgta
     6061 gccacgtgac tcgtcggtgt actcgatcaa ggctccagcg gaccaggtga tgggtccgcg
     6121 gtcgttctgg ccctgcccgt tggcatcgaa gcctgagcgg tcgcgcagct cgaggtagta
     6181 ggcgtgatcg agggtggacg gcgatgtggc gtcgaccagc gaccagccgt tcgtgcactt
     6241 gtcggcgacg cgcaacccgc cgtcgccctg gcagccgccg gggaacacac gcacgtcatc
     6301 gtcgttggtg aaattgttcg agtagatcgt ctgcgagccg gccttgagga cgaggtcgtc
     6361 catgaaccag cccggccgat cgagaccagg gtcggtgtag tacgaaaatc ggaggacgac
     6421 gccgctctgg cccgcgtacg cggaaaggtc gtactcgtcc tgaaggaacg gcgagtcctt
     6481 atagatcggg ctggccgggt tgcggtccac cgcgaacgtg ctgtcgcgat atgacccgct
     6541 ggtgccggtg atcccgttgc cgtagcgctc ctggcaggct gaattgttcg ggttgatgct
     6601 gctcggggtt gtgtactgct ttgccgaagg tagcgaggtg tagtccttgc cgttggtggt
     6661 gaccatcgtg aagccgtagt cgaagtccca ctcgatatcc cacttggact tgaaggtcag
     6721 cgtgatcgga gtgccatcag ggaccgtcgc gagctcaggc agcgcgatat cgagattgtg
     6781 gccgccgact ggcgtgcagc caaaatcgtt gccgcggcct gagtaccaga cgttggggag
     6841 cgatgcttca gcctggacct ggctggactc gatcagcttg cgtcgcggca gcttgagccc
     6901 gaacgcctcg ccgttgtgga tgttttggtc gccgttagcg ctggacagcg tgtagaacgt
     6961 cccgtcgggt cgctgccagt ggatttcgcc agtgtcgttc ttgatctcct cccaatttgt
     7021 gacggtgcgc gtctcgccgg gctggaggaa ttcgggcaca acccagccca gatcctgctt
     7081 gctgaagacc gtcatgttct gcgagaagtc ggtcgccatg agattccagt cgttgtaggt
     7141 cgtgtagctg gttgagtagt agtcgggcag acccaggtgg tggccatact catgcgagat
     7201 gacactggcc tggtcgattg ccgactcggg attcacgttg taggggccga cgcgaacagg
     7261 gaccggcttg tcgttctgcc ctgtgccgcc attggccgcg caggcgtcaa acgcggtgta
     7321 ggtggcgtcc ttgaagcact ggggcacgcc cttgaggctc ttcagctgat cgtcagagac
     7381 aaagcccttg agacccgtga ccggctggct gtatgagtat tcgaggctgg agctgtgcgg
     7441 ccagatgttg tcgtacggag ccggctcgcc gttgatctgc gagtcgccat tgccgccctt
     7501 gccgacaaac acgagcatga agaagtcgac gacgccgtcc ttatcgaggt cgtagtcgtt
     7561 gtagtcgatc tctgggtcgg ccacgacggc ggcgtcgtaa acggcctgac cggtggggcc
     7621 gcaggcgccg tcgatctg
//
LOCUS       DATLCO010000173         3417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4701229, whole genome shotgun sequence.
ACCESSION   DATLCO010000173 DATLCO010000000
VERSION     DATLCO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4701229"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VMZ33_04355"
     CDS             complement(<1..753)
                     /locus_tag="VMZ33_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16491.1"
                     /translation="MLRMHSSSRAFAGVVLVLLAASPVSAGTAPVTLPAAPNCPIFPS
                     SNVWNKPVDGLDVAGNSGTLMRSIGLESYLHPDFSSIAGGNYGIPINVVGSGARRVNV
                     RFRWPGESDPNPYLMPRNPKFESGSDRHLIVVDRDACKLYELFAMRKTSTGWRAGSGA
                     KWDLRSNALRPDGWTSADAAGLPILPGLARYPEVAAGEIRHALRFTVPSTRSSYIYPA
                     RHFASASNDPALPPMGVRLRLKDGVDISAFGPQ"
     gene            1043..1222
                     /locus_tag="VMZ33_04360"
     CDS             1043..1222
                     /locus_tag="VMZ33_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16492.1"
                     /translation="MRLIRFLFLLLLVLVAVVLVVPLIVLAGLFVWLKLTEETDEEAL
                     ELEPEMETATDTASV"
     gene            1273..2073
                     /locus_tag="VMZ33_04365"
     CDS             1273..2073
                     /locus_tag="VMZ33_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HUR16493.1"
                     /translation="MPPPEAGGAPVSFLVDYDGTIAIDVGDELLKRHYDNKAEIARLD
                     ALYDDAKIGSRELMRWDMDVLPRDADMLRRDAAAMPQDETFRAFVEGVRGHGAVVEIV
                     SDGLGFYVQPNLAQLGLGDLFVATNENRVENGGEGLSFPFGHPSCFVCGTCKRERVRL
                     HQAAGRVVVFIGDGTSDRYAAAHADLVFAKGSLARICADQDWPFHEWDRFDQVLAEVA
                     RAFADGRLPRDVEDLPVWRGRHAHAARSFICGPEVWGPGRTTPGPGPH"
     gene            2104..2697
                     /locus_tag="VMZ33_04370"
     CDS             2104..2697
                     /locus_tag="VMZ33_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HUR16494.1"
                     /translation="MGRMSTLVSMLLAFALAATVLAAALLVGLFPGGGGTAVATTRPP
                     QASGPLVTSPPVATPSPTPTPIPTQTDAGPQTIPPEGTTYTVQPGDALSLIGERFEIS
                     WLLIAQANNIPPPDYIITPGQVLIIPAAGPEPSAGAEFYIVQSGDTITSIAQAVGIEP
                     TALADFNNIADWNTIQVGQVLYIPSDVDATPLPRETP"
     gene            2834..>3417
                     /locus_tag="VMZ33_04375"
     CDS             2834..>3417
                     /locus_tag="VMZ33_04375"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HUR16495.1"
                     /translation="MAKYVFVTGGVTSSLGKGITAASIGRLLKSRGLSVSIIKLDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSQLSNVTTGRIYQAVIAKE
                     RRGDYLGGTVQVIPHITNEIKERVLRVSRDAKADVVIVEVGGTVGDIESLPFLEAIRQ
                     MRKDVGRGNVLYVHVTLLPALSATGELKTKPTQHS"
BASE COUNT          610 a         1145 c         1094 g          568 t
ORIGIN      
        1 ctgtggcccg aaggcggaga tgtcgacgcc gtccttgagc cgcagtcgga ctcccatcgg
       61 cggcagcgcg ggatcgttcg acgcggaggc gaaatgccgc gccgggtaga tgtatgagct
      121 ccgcgtcgac ggcaccgtga agcgcagcgc gtgccggatc tctccggccg ccacttccgg
      181 gtagcgcgcc agcccgggca ggatcggcag cccggccgcg tccgcggaag tccagccgtc
      241 gggtcgcagg gcgttcgagc gcaggtccca tttggcgcca gaaccggcac gccagcccgt
      301 agacgttttg cgcatcgcga agagctcata gagcttgcaa gcgtcgcgat cgaccacgat
      361 caggtgccga tcggagccgc tctcaaactt cggattgcgt ggcatgaggt atgggttggg
      421 gtcggactcg cctggccatc ggaagcggac attgacccgc ctcgcgcccg accccacgac
      481 attgatcggg atcccgtagt tgccaccggc gatggacgag aagtcaggat ggaggtacga
      541 ctcaagccct atcgaccgca tcagcgtccc tgagttgcca gcgacgtcga gcccatccac
      601 aggtttgttc cagacgttgc tcgagggaaa aatcgggcag ttcggagccg ccgggagggt
      661 caccggagcc gtccccgcgc tgaccggcga ggcagccaga agcacgagga cgacaccggc
      721 aaacgcacgg ctggatgaat gcatacgaag cacgaccaga tgctcctcaa ggccacgtcc
      781 acacggcaag ggcgcgtcgg tccagttcgg cgcgtaccta agtccggcgc acggaggcac
      841 cgttggctgc tgagcgcgcc ccggcagacc gcgccccggc agaccgcgcc ccggcagacc
      901 gcgcctccca atccgcgttg aaaggcagac ttgagccgcg tgccaggttc cctgatcgtg
      961 cgcgcagccg gattgcgcta ccgtgaaccc gaaaccaaat tcgaaacact gcccggggcc
     1021 cgctcccatc ggaggtcgca agatgcgatt gatcagattc ctgttcctgc tgctactcgt
     1081 cctcgtcgcg gtcgtgctgg ttgtgccgct catcgtcctt gccggactct tcgtatggct
     1141 gaagctgacc gaagagacgg atgaggaggc gctcgagctc gagcccgaga tggagacggc
     1201 aaccgacacc gcgtcggtct gaacaagtct ctcgacgggc agactgaccg cccgatcgaa
     1261 cacagcgcac acatcccgcc gcccgaggca ggcggcgcgc cggtcagctt ccttgtcgac
     1321 tacgacggca cgatcgccat cgatgtcggc gacgagctac tcaagcggca ctacgacaac
     1381 aaggctgaga tagcccgtct cgacgcgttg tacgacgatg ccaagatcgg ctcgcgtgag
     1441 ctcatgcgct gggacatgga cgtcctgccg cgcgacgccg acatgttgcg acgcgacgcc
     1501 gcggccatgc cccaggacga aactttccga gcgtttgtcg aaggtgtccg cggacacggt
     1561 gcggtggtgg agatcgtgag cgacgggctc ggcttctatg tccagccaaa tctcgcgcag
     1621 ctcggtctgg gcgatttgtt cgtcgccacg aacgagaatc gggtcgagaa cggcggggag
     1681 ggcttgtcct ttccatttgg ccacccgagc tgtttcgtgt gcgggacgtg caagcgcgag
     1741 cgggtccggc ttcaccaggc tgcggggcgg gtggtcgtat tcatcggtga cggcaccagc
     1801 gaccggtatg cagccgctca cgccgacctg gtttttgcca aggggtcgct ggcgcgcatc
     1861 tgtgccgatc aggattggcc gtttcacgaa tgggaccggt ttgaccaggt tctggcggag
     1921 gtggcgcgag ccttcgcgga cggccggtta ccgcgcgatg ttgaggacct gccggtctgg
     1981 cgtggacggc acgcccatgc cgcgcgatcg ttcatttgcg gccccgaagt ctggggcccg
     2041 ggccgcacca caccgggtcc gggaccgcac taaaccctca cccggcctat gatcggccgc
     2101 gttatgggac gcatgtcaac gctcgtcagc atgctgctgg cgtttgctct cgctgccacg
     2161 gtcctcgccg cggcgctgct cgtgggtctg ttcccgggtg gtggcggcac ggccgtcgca
     2221 acgacgcgtc cgccccaggc atcaggcccg ctggtaacga gcccgcccgt ggccactccc
     2281 tcgccgacac ctacgccgat tccgacgcaa accgacgcgg gtccgcagac gatcccgccc
     2341 gaggggacga cttacaccgt gcagcccggc gacgcgctgt cgctgatcgg cgagcgcttc
     2401 gaaatttcgt ggttgctgat cgcgcaggcc aacaacatcc cgccgccgga ctacatcatt
     2461 acgccgggcc aggtcctgat cattccggcc gctggcccag agccgtcggc cggcgccgag
     2521 ttctacatcg tgcagtcggg cgacacgatc acgagcattg cgcaggcggt aggcatcgag
     2581 ccgactgcgc tggccgactt caacaacatc gccgactgga acacgatcca ggtcggccag
     2641 gtgctgtaca tcccgtccga cgtcgacgcc acgccgcttc ctcgcgagac gccctgaaag
     2701 actgaggccg cgagtctgtc caagatctcg cgcaatttgc gaagggtctc ctccccgcag
     2761 tcgcctgccg gcgcgccctg cggcgtcgga cgctttggct taacattcga aaaacccccg
     2821 gaggtccgaa tctatggcta agtatgtctt tgtgaccgga ggcgtcacct cctcgctcgg
     2881 caaaggcatc accgccgcca gcatcggacg gctcttgaag agccgcggcc tctccgtctc
     2941 gatcatcaag ctagatccct atatcaacgt ggatccgggc acgatgtcgc cgtaccagca
     3001 cggcgaggtc ttcgttaccg atgatggcgc ggaaaccgat ctcgatcttg gccattacga
     3061 gcgtttcatc gacgagaacc tctcgcagct gagcaacgtc acgaccggcc gcatctacca
     3121 ggcggtcatc gccaaggagc ggcgcggcga ctacctcggc ggaaccgtcc aggtcatccc
     3181 gcacatcacc aacgagatca aggaacgcgt cctccgcgtg tcgcgcgacg ccaaggcgga
     3241 cgtcgtgatc gtcgaggtcg gtggcaccgt cggcgacatc gagtcactcc cgttcctgga
     3301 ggcgatccgc cagatgcgca aggacgtcgg ccgtggcaac gtgctgtacg tccacgtcac
     3361 cctgttgccg gcactcagcg caacgggcga gctcaaaacc aagccgaccc agcactc
//
LOCUS       DATLCO010000174        11307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2472161, whole genome shotgun sequence.
ACCESSION   DATLCO010000174 DATLCO010000000
VERSION     DATLCO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472161"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VMZ33_04380"
     CDS             <1..570
                     /locus_tag="VMZ33_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16496.1"
                     /translation="AGEGLQGGFSAVYPILREMEERGRVRRGYFIEGLGGAQFCVPAA
                     LDRLRAERSDPSGDMERDTEALILAASDPANPYGSALTWPRWSDEDRRPLPRAAGAYV
                     VIVDGDPVVYLDRGGKSLQTLPAFADRATAQRALLALRQLVADGRYRTLQIERVDGTP
                     VSESAHAEALGEAGFQRSYRGWLLKPANA"
     gene            603..1418
                     /locus_tag="VMZ33_04385"
     CDS             603..1418
                     /locus_tag="VMZ33_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HUR16497.1"
                     /translation="MPEGDTLARIANSLRPVLLDKRIEAARGRPGGADLSRVVGHIVT
                     NVQARGKHLLIAFDNDLTLHTHLGLHGSWHRYRRGQIWRRAVTRTGAVLETDEWVAAC
                     FDAPTVELIETRAVAIHPGIRDLGTDLASDEFDPDAALAGLRDASRRTLAVGDALLDQ
                     GALAGLGNVYRSELCFIERVNPLTPVPEVTDDKLRVMAIRGAILVKQNSRGGSRVTTA
                     AGTPSNTYVYGRTGRPCFRCRTRIQSTVTRARETSNPRRVYWCPGCQPPTSHS"
     gene            1515..2966
                     /locus_tag="VMZ33_04390"
     CDS             1515..2966
                     /locus_tag="VMZ33_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16498.1"
                     /translation="MPGQLRARVGVAMMLGALWVSALAAPVSAAGAVRWVDNDSNPGD
                     GPTKCDTAPYHTIQSAINNSNPYDTIYVCPGTYRQALHVDVKGLHIESINYRKAKIMP
                     PTSYGANFPALVSISARDVEFWNFLMPIAGGPLQIVGPFVGGGGGVGPSCETTFVAIE
                     VLGPNADIRGNYLKGTGDYTFSGACGYLYGIVVAAPSLPGMIPDVSGPNVSSISHNRI
                     LDFKYAGVLAVGDVQARIVRNAVRFVHEDDPATCVLTPVLGVTAELTFPCQVETARIE
                     TGVNGAFSQAIGIGAEGGPLVEIRNNTVYSTFDQSLLIGLPVQGGIPGPGPDTSIFLS
                     GGIAMLDVADGSTARNNTVSNVGFGLLVANGGISGVTLAEPADNPNGSKFTGNRLYDS
                     FFGLAVAGDDNVFYGNRARTNFAGTFVIAGQNNLFHANDFRFNMEIDCFDGTTGTGDA
                     GTANLWTNGSAGRYNFGQYNQPEEICIPTLNPI"
     gene            complement(3104..4570)
                     /locus_tag="VMZ33_04395"
     CDS             complement(3104..4570)
                     /locus_tag="VMZ33_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HUR16499.1"
                     /translation="MALLGALALASSSVLAAPVIQSLGDLNVASGDGDPSNFTKFGTK
                     ILFVATDETKGREIWRTDGTTAGTRIIKDINPGGDSWPNGFFIVNGVALFSADNGGGD
                     ELWRTDGTAAGTKKVKDINGDPNTGSLDGISARYGSYLYFYADDGVHGIELWRSDGTA
                     AGTKMLKDVNVGSGDSDRCCPRQLGNIVLFQAFDPAHGYELWRTGGTPGTTTFVADVN
                     SGPNASYPLDDGVVAGSRYFFCAWPTSDLWVTDGTPGGTALVKDFDYCVNPHPYGNDV
                     LVTARSVGSSVELWRSDGTPGGTAMVTDLNPTGDSYPNLINDQPVNGKYFVAADDGTH
                     GRELFVYNGSTLSLVEDINPSGGSGIGQTGNRRMALGNRFVFEADDGVHGYEPWITDG
                     TAPGTFLLKDIAPGLPISESYAEVALNGMLYFSAENATYGRELWRTNGTQLGTKRITD
                     LNPGGGGSWPNGLTAINGKIYLSADDGTHGFEPYRLTP"
     gene            complement(4710..5471)
                     /locus_tag="VMZ33_04400"
     CDS             complement(4710..5471)
                     /locus_tag="VMZ33_04400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HUR16500.1"
                     /translation="MFAFIYARGELAGADALAYWTGVQRWLRGEDIYQVIPGLYVAPR
                     DGALPYAYAPWSLYVFLPFGLIPWDIVWPLWRAANLVLFALSVGWAYQRRPLATAALV
                     AVLGPALAANLDTGNINIVLALMVWAAWWSSPQIGGFLWALGAGLKFVPAALLPFVPT
                     RAWGYGFMTLGVMILLTLATWPQTVRQLDIVINYPRPLRIDYMMLLWAVVPWLYSRPL
                     RPRLDRDWLAVVNRAKREAVGDTVVTEPASSQFDT"
     gene            5746..6582
                     /locus_tag="VMZ33_04405"
     CDS             5746..6582
                     /locus_tag="VMZ33_04405"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HUR16501.1"
                     /translation="MTEGHVIIIGGAEEKIGERVILTRFIELAGGTDARIAVISSASS
                     LGPLAGEMYRRVFTELGAAEVHPIHAVTRAQANNANNAQTIKNATGVFLTGGNQLRLS
                     STIGGTAIAQQIQRRHREGAVIAGTSAGASAMSTHMVAFGASGATPKQRMVQMAAGLG
                     ILPGVIVDQHFEARNRLGRLLAIIAANPSLLGIGVDEDTAGVVGPDRVLEVVGRRSVT
                     IIDGAESDTDAWEVKAHRPVMISNVVLHSLPQGYRFDLERRERVVPPMLRALESDQLA
                     SS"
     gene            6591..9419
                     /gene="cphA"
                     /locus_tag="VMZ33_04410"
     CDS             6591..9419
                     /gene="cphA"
                     /locus_tag="VMZ33_04410"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HUR16502.1"
                     /translation="MASTTKPATRGGRRPKPVKGAVTNAEHPHPTLRILDTRVLRGPN
                     VWSRSPAIVMKVDLGELEEWPSNRIPGFNKALLEVLPTLDSHSCSLGRRGGLVTRLHD
                     GTWLGHVSEHIAIELQSLAGTEVHLGKTRGAGATGRYNVVYEFREEQVGLEAGRIAVA
                     LVNHLASPEEAPLDFAAALEDLIRLAERQAFGPSTQALVDEAVSRDIPFMRLDRHSLV
                     QLGQGVHQQRIRATMTSRTGAIGVDIASDKSLTNSLLSSAGLPVPKADTVRRVEEGVA
                     AANKIGYPVVVKPLDGNHGRGVALDLRTDDDVRRAFDLAYNESRNGDVVVESYVTGND
                     YRILIIGGKLAAVAERVPASVVGDGQHTVRQLVESENADPRRGIGHEKVLTRIRVGPA
                     AEELLAKQGLSLDEVPQKGQRVLLAATGNMSTGGTSIDKTREAHPDNVEIAEMAAQVV
                     GLDVAGIDFIAPDISVPVREQGGAIVEVNAAPGFRMHTHPTVGEPQWVARPVIDLLFP
                     NGSNARIPIVAVTGTNGKTTTVRMISHIQKLMGRRVGMTSTDGIVVDGRVIRKGDMSG
                     PRSAQMILQNPSVDSAVFEVARGGILREGLGYDRNDVAVVTNVTGDHLGLKGVDTIDK
                     LAQVKSVLVEAVPRAGFAVLNADDPLVAKMARQCRGTVVYFSMAAPGTAEADRVSGHC
                     GRGGAAMVVQQADDGELMVLRHADRTMAILYTHLIPATFGGRARMNVANALAAAAAAW
                     SVGAHLHDIRQGLRTFSTSFFQAPGRLNEMNIDGYKVILDYCHNVDGMRRLTEFVDLM
                     VGATNDGVKRPGGVSGRAIGVIGMPGDRRDDDHREYGRLAAHSFDAVIVREDKNTRGR
                     ERGQSADLVAEGVRAGMAEGGRCSDVRIVVNEQESAIAGLQLAERDDLVVICADDITG
                     VYRKVMETAKSNGGAAFADPGEFSAEEG"
     gene            complement(9424..10221)
                     /locus_tag="VMZ33_04415"
     CDS             complement(9424..10221)
                     /locus_tag="VMZ33_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16503.1"
                     /translation="MACYFDGDVVAVDPVNGEVVDRVHVGIGAIGITLAAGSLWVSVH
                     ESAEVARVNPETHEVIAVIDVGPQPEGIAGDEAGIWVTSEEDGAVYRIDPLTNEVVDT
                     VEVGREPRHVELGFGSVWVTNFGSATITRVDPATNEVVATIEGGFGPEGIAFTEDMVW
                     PDIAWVANVRDGTVSQIDPETNQVIGTVQTGAGSEGLMVDEAGNLWVAVAGQGEVAVI
                     AGASPDDVLDRYTAGAEPRRLVFAEDRLWVTNTGDGTLSSLQVPIPE"
     gene            10499..11002
                     /locus_tag="VMZ33_04420"
     CDS             10499..11002
                     /locus_tag="VMZ33_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HUR16504.1"
                     /translation="MSTTERDATSDELKALGHPLRWRILRVTLDHPLTNKEIAARLGR
                     DPGTTLHHVRVLADAGFLAPERVRAGARGALERPYRNTGKSWRIRLAPRADNTVAILD
                     AAREEILEAGPDAAISTLRLAVNLKAADLEELSRRISDLGDEFAGRGDPDGQPLGIVA
                     LVHRRRP"
BASE COUNT         2010 a         3694 c         3679 g         1924 t
ORIGIN      
        1 gccggggagg ggctgcaggg cggcttctcc gccgtctacc ccatcctgcg cgagatggag
       61 gagcgcggcc gtgtccgtcg cgggtacttc atcgaaggtc tcggcggcgc gcagttctgc
      121 gtgcctgccg cgctcgatag gctgcgtgcc gagcggtccg atccgagcgg cgacatggag
      181 cgcgataccg aagcgctgat cctggccgcg agcgacccgg caaatccgta cggctcggcg
      241 ctcacgtggc cgcgctggtc ggacgaggac cgccgtccgt tgccgcgcgc cgccggtgcg
      301 tatgtggtca tcgttgacgg cgatccggtc gtctatctcg accgaggcgg taagtcactg
      361 caaaccttgc ccgcgttcgc cgatcgcgcg acagcgcaac gcgccttgct ggctctccgg
      421 cagctggttg ccgacggtcg ttatcggacc ctccagatcg agcgcgttga cggcacgccg
      481 gtgagcgaat ccgcgcacgc cgaagcactg ggcgaggcag gcttccagcg gagctatcga
      541 ggctggctcc tcaaaccggc aaatgcctga ggccggcaca aaatccctga gggcgacacg
      601 gaatgcctga gggcgacacg cttgcccgga tcgccaactc gctccgaccc gtcctgctcg
      661 acaagcggat cgaggctgca cgtggacgcc ccggcggcgc cgacctgtcg cgggtggtgg
      721 gtcacatcgt gaccaacgtc caggcacgcg gtaagcacct gctcatcgct ttcgacaatg
      781 atctgacgct ccacacccat cttgggctgc atggctcgtg gcatcgctat cggcgcggtc
      841 agatctggcg gcgcgcagtc acgcggaccg gcgccgtgct cgaaaccgac gagtgggttg
      901 ccgcctgttt cgacgcgccg accgttgagc tgatcgagac gcgtgctgtg gcgatccatc
      961 ctggcatccg cgatctcggt actgacctgg catcagatga gtttgacccg gatgcggccc
     1021 ttgccgggtt gcgcgacgcg tcgcggcgaa cactggccgt cggggacgcg ctgctggacc
     1081 agggcgcgtt ggccggcctg ggcaacgtct accgcagtga gctgtgcttc atcgagcgcg
     1141 tcaacccact gacgcctgtc cctgaggtca ctgacgacaa gctccgagta atggcgattc
     1201 gtggcgccat cctcgtcaag cagaattcgc gcggtggttc gcgcgtgacc actgcggcgg
     1261 gcacgccctc gaacacctac gtttacggtc gtaccggtcg gccgtgcttt cgctgccgga
     1321 cgcgcattca gtcgaccgtc acccgtgccc gcgagacctc caatccgcgg cgcgtttact
     1381 ggtgtccagg ctgtcagcca ccgacgtcac attcatgaca tacgtcatat tgggtatgcg
     1441 ctgagcgtcc acggaaccta acatccgcac cggaccggcc cacgtcacgg ccggcggttc
     1501 gaaggggagg gcaaatgccc ggtcagctac gcgccagagt cggcgtcgcc atgatgctcg
     1561 gtgcgctctg ggtgagcgct ctcgcagcac ccgtcagcgc tgccggcgct gtccgatggg
     1621 tcgacaacga cagcaaccca ggagacggcc cgaccaaatg tgacacggcg ccgtatcaca
     1681 ccatccagtc ggcaatcaac aactcgaacc cgtacgacac catctacgtc tgtcccggca
     1741 cctaccgcca ggcgctgcac gtggacgtca agggtctgca tatcgagtcg atcaactatc
     1801 gcaaagcaaa gatcatgccg ccgacgagct acggggcgaa cttcccggcg ctcgtctcaa
     1861 tcagcgcgcg cgacgtcgag ttctggaact tcctgatgcc gatcgctggt ggcccgctgc
     1921 agatcgtggg gccgttcgtg ggtggtggcg gcggtgtcgg cccgagctgc gagacgacct
     1981 tcgtcgcgat tgaagttctg ggtccaaacg cagacatccg cggcaactac ctcaagggca
     2041 ccggtgacta cacgttcagc ggcgcctgcg gctacctgta cggcatcgtg gttgctgcgc
     2101 cctcactccc ggggatgatt cctgacgttt caggtccgaa tgtctccagc atctcgcaca
     2161 accgcattct tgatttcaag tacgcgggcg tcctcgccgt gggtgatgtc caggcgcgta
     2221 tcgtccgaaa tgcagtgcga ttcgtccacg aggacgaccc cgccacatgc gtcctgactc
     2281 ccgtcctggg cgtgacggcg gaactcacct tcccatgcca ggttgaaact gcgcggatag
     2341 aaaccggggt taacggagcc ttcagtcagg ccattgggat cggggccgag ggcggtccgc
     2401 tcgtcgagat ccggaataac accgtctatt caaccttcga ccagtcgctc ttgatcggct
     2461 tgcccgtgca gggcggcatc cccggtccgg gacctgacac ctcgattttc ctcagcggcg
     2521 gcatcgccat gcttgatgtc gcggacggct cgacggcacg caacaacacc gtgtcgaatg
     2581 tcggatttgg ccttctcgtc gccaatggcg ggatcagcgg cgttaccctg gctgaaccag
     2641 ccgacaaccc caacggctcc aagttcaccg gcaaccgtct gtatgacagc ttcttcggcc
     2701 tcgccgtggc tggcgacgac aacgtgttct acggcaaccg cgctcggacg aacttcgccg
     2761 gcaccttcgt gatcgctggc cagaacaacc tgttccacgc caacgatttc cgcttcaaca
     2821 tggagattga ctgcttcgac gggacaaccg gtacgggcga tgcaggaact gcgaacctgt
     2881 ggaccaacgg atccgccggt cgctacaact tcggccagta caaccagccc gaggagatct
     2941 gtatcccaac gctgaacccg atctaacggc taaggctcag cgctctgtga gagggcggtc
     3001 gctcccggtt cgccggggcg gccgccctat ctgtttgccc gacacgctca gcgggccact
     3061 catacgccgc agaacccggg tgatcggacc gccgggcggc tcgctagggt gtcagccggt
     3121 atggctcgaa gccgtgcgta ccgtcgtcag ccgagagata gatcttgccg ttgattgcgg
     3181 tcagcccgtt gggccaagat ccaccgccgc ccgggttcag atccgtgatc cgtttcgtcc
     3241 caagctgggt accgttcgta cgccacagtt cgcggccgta cgtcgcgttc tcggcggaaa
     3301 aatagagcat cccgttcagg gcaacttcag cgtatgactc agagatcgga agacctggag
     3361 cgatgtcctt gagcaggaag gttccaggcg ccgtgccatc ggtgatccat ggctcatacc
     3421 cgtggacgcc atcgtccgct tcaaatacga acctgttccc gagtgccatc cgcctgttgc
     3481 cggtctgtcc gatgcccgag ccgcctgacg gattgatgtc ttcgaccagg gacagggtcg
     3541 acccgttata gacgaatagc tctcgcccgt gcgttccatc gtccgctgcg acgaagtact
     3601 tgccattgac gggctggtcg ttgatcaggt tcgggtaaga gtcgccggtc gggttcaggt
     3661 ccgtgaccat ggcggtgccc cccggcgttc catcgctgcg ccagagctcg acgctcgaac
     3721 cgacggagcg cgctgtcacg agaacgtcgt tgccgtaggg gtggggattg acgcagtagt
     3781 cgaagtcctt taccagtgcc gttccgccgg gcgtgccatc cgtcacccac aggtcgctgg
     3841 tcggccacgc gcagaagaag taccgactcc cagccacgac accgtcatcc agcggatacg
     3901 acgcgttggg cccggagttg acatcggcaa cgaaggttgt cgttcccggt gtgccacctg
     3961 tcctccacag ttcatatccg tgcgccgggt caaatgcctg gaacaggacg atgttgccga
     4021 gctggcgggg gcaacacctg tcggaatcgc cagatccgac gttgacatct ttgagcatct
     4081 tcgttcctgc ggccgtgccg tctgaacgcc agagctcgat gccgtgcacg ccatcgtcgg
     4141 cgtagaagta gagatagcta ccgtaccgag ccgagattcc gtcgagggac ccggtattcg
     4201 gatcaccgtt gatgtccttg accttcttcg taccggctgc agtgccatca gttcgccaca
     4261 gctcatcacc gccgccgtta tcggcagaga agagtgcaac gccgttgacg atgaagaagc
     4321 cattcggcca cgagtcgccg ccaggattga tgtccttgat gatcctcgtg cccgccgtcg
     4381 tgccgtccgt ccgccagatc tctcgaccct tcgtctcgtc agtagcgacg aacaggattt
     4441 ttgtgccgaa cttcgtgaag ttagaagggt ctccgtcgcc cgacgcgaca ttcaggtcgc
     4501 cgagactctg gattacggga gcggccagaa cgctcgagct cgccaacgcg agcgcgccca
     4561 gaagcgccat ggcagaggcc atgatggacc gccgtggtcg gctgatgccc agccctcgac
     4621 tcaacatgat cattcctcca ccgcggtgcg gtcgactgcc cctaccaagc tttacgcctg
     4681 cccacgatac cgaggccgcg tggtcaaggc taggtgtcaa attgcgaact ggcgggctcc
     4741 gttacgactg tgtcccccac ggcctcgcgt ttggctcggt tgacgaccgc aagccagtcg
     4801 cggtccaggc gcggccgcaa cggacggctg tacagccacg gaacgacggc ccaaagcagc
     4861 atcatgtagt cgatccgcag cgggcgcggg tagttgatga cgatgtcgag ttgcctgacc
     4921 gtctgcggcc aggtagccag cgtgagcaga atcatcacgc cgagcgtcat gaatccgtag
     4981 ccccatgccc gggtgggcac aaacggcaac aaggcggcgg gcacgaattt gaggcccgca
     5041 ccgagtgccc agaggaagcc cccgatctgt gggctcgacc accacgccgc ccagaccatg
     5101 agggcaagaa cgatgttgat gttgcccgtg tccaggttgg ccgcgagtgc cgggcctaga
     5161 acggccacga gcgcggcagt ggccagcggt cgtcgctgat acgcccagcc gactgacaac
     5221 gcgaacagga cgaggttggc cgcgcgccac agcggccaga cgatgtccca ggggatgagg
     5281 ccgaacggca ggaacacgta cagggaccac ggcgcgtacg catacggcag tgccccgtcc
     5341 cttggcgcga cgtacagccc ggggatcacc tggtagatgt cctcgccgcg caaccagcgt
     5401 tggacaccgg tccagtacgc cagcgcatca gctccggcga gctcgccgcg ggcgtagatg
     5461 aacgcgaaca cgaggccgcc ggtgagagcc agaagcgcga tcgctagaag gcggcgcggt
     5521 tggcgccaga atgcgattgg gccaggcgca ccgccggtgt aggaaagagg ttgggcgggg
     5581 ctgcgttcgt cctcctcggc cgaacgtgtt gggcgccccc gcccggcgcg catgttagcg
     5641 acttgccaca agtcggcagc atcggtggtg cgcgcagcaa gcatgtctct gtgagtatcc
     5701 atgggtggca agttgtatcg gggcagaaat gccatactcc ggcggatgac cgaggggcac
     5761 gtcatcatca tcggtggcgc agaagaaaag atcggagagc gggtgatcct cactcgcttt
     5821 attgagcttg ccgggggaac ggacgcgcgg atcgcagtaa tttcaagcgc atcgtctctt
     5881 ggcccgctgg cgggcgagat gtaccgacgt gtcttcaccg agctcggagc cgccgaggtt
     5941 caccccatcc acgccgtcac gcgcgcgcag gcaaacaacg ccaataacgc gcagaccatc
     6001 aaaaacgcca ccggtgtctt cctgacgggc ggcaatcagc tgcgcctctc gtcgactatc
     6061 ggcggcaccg ccattgccca gcagatccag cggcgtcacc gcgaaggcgc cgtaatagct
     6121 ggcacgagcg ccggcgcctc cgcgatgagc acgcacatgg tcgcatttgg cgcaagcggc
     6181 gcgacaccga aacaacgcat ggtccagatg gcggccgggc tgggcatcct gcccggcgtg
     6241 atcgtcgatc agcatttcga ggcccggaac aggttggggc gcctgctggc gatcatcgcc
     6301 gccaatccgt cgctcctcgg aatcggggtc gatgaggaca cggctggcgt agtcggacct
     6361 gatcgcgtcc tggaggttgt cggccgtcgc tccgtgacca tcattgatgg ggccgagtcc
     6421 gacacggacg cctgggaagt caaggcgcac cggccggtga tgatcagcaa cgtcgtcctc
     6481 cattcgctgc cacagggcta tcgcttcgat ctcgagcgcc gcgagcgcgt cgtcccccct
     6541 atgctgcgcg cactggagtc agaccagctc gcttccagct aggagcacga gtggcaagca
     6601 ccaccaagcc cgcgacccga ggagggaggc ggcctaagcc agtcaagggc gccgtcacga
     6661 acgccgagca tccgcatccg acgctgcgca tcctcgacac gcgggtcctg cgcggaccga
     6721 acgtctggtc acgctctccg gcgatcgtga tgaaggtgga cctcggcgag ctcgaggagt
     6781 ggcccagcaa ccgcatcccg ggcttcaaca aggcgctcct ggaagtgctg ccgacgctgg
     6841 actcgcactc gtgttcgctc gggcggcgcg ggggcctcgt cacacgactt cacgacggaa
     6901 cgtggcttgg ccatgtgtca gagcacatcg cgatcgagct gcagagtcta gccgggaccg
     6961 aggttcatct gggcaagacg cgcggcgccg gcgccaccgg tcgctacaac gtcgtctacg
     7021 agtttcgcga ggagcaggtc gggctcgagg ccggccggat cgcggtcgcc ttggtcaacc
     7081 atctcgcgtc gcccgaggag gcgccgctcg atttcgccgc ggcgctcgag gacctgattc
     7141 ggctggcgga gcgccaggca ttcggaccgt caacccaggc gctcgtcgac gaggcggtgt
     7201 cacgcgacat cccgttcatg cgcctcgacc gtcactcgct cgtccagctc ggtcagggcg
     7261 tccaccagca gcgcatcagg gcaacgatga cctcgcgcac tggcgcgatc ggggtcgata
     7321 tcgcctccga caagagcctc accaactcgc tcctgtcttc cgccggattg ccggtgccca
     7381 aggccgacac ggtccggcga gtcgaggaag gcgtcgccgc tgccaacaag atcggctacc
     7441 ccgtcgtggt caagccgctc gacggcaatc acggccgtgg cgtggccctc gatctgcgca
     7501 cggacgacga cgtccgccgg gcgttcgacc ttgcatacaa cgagtcgcgc aacggtgatg
     7561 tcgtcgtcga gtcgtatgtg accggcaatg actaccggat cctgatcatc ggcggcaagc
     7621 tcgccgcggt cgcagagcgc gtcccagcga gtgtcgtggg cgacgggcag cacaccgtgc
     7681 gccaactggt cgagagcgag aacgcagatc cgcggcgcgg catcggccac gagaaggtgc
     7741 tgacgcggat tcgcgttgga ccggcggctg aggagctgct cgccaagcag ggcctgagcc
     7801 ttgatgaggt gccacagaag ggccagcgcg tccttctggc tgcgacgggc aacatgtcga
     7861 cgggtggcac gtccatcgac aagacacgcg aggcgcaccc cgacaacgtt gagatcgcgg
     7921 agatggctgc gcaggttgtg ggcctggatg tcgccggcat cgacttcatc gcgcccgaca
     7981 tcagcgtgcc ggtacgcgag cagggcggcg cgatcgtcga ggtcaacgcc gcacccggct
     8041 tccggatgca cacccatccg accgtcggtg agccgcagtg ggtggctcgg ccggtaattg
     8101 atcttctctt cccgaacggc tccaacgcgc gcataccgat cgtggctgtg accgggacga
     8161 acggtaagac gacgacggtt cgaatgatca gccacatcca gaaactgatg ggccgacgag
     8221 tcggtatgac ctctaccgac ggaatcgtgg tcgacggtcg cgtgatccgg aagggcgaca
     8281 tgtccggacc gaggtcggcg cagatgattt tgcagaatcc gtcggtcgac tctgctgtct
     8341 tcgaggtcgc tcgcggcggc atcctgcgcg aagggctcgg ttacgaccgc aacgacgttg
     8401 cagtagtgac caacgtgacc ggtgaccacc tcggactgaa gggcgtcgac acgatcgaca
     8461 agctggccca ggtcaagtcg gtcctcgtgg aggctgtgcc ccgggcaggc tttgcggtgt
     8521 tgaacgcaga cgatccgctc gtcgccaaga tggctcgcca atgccgtggc acggtcgtct
     8581 acttctcaat ggcggcgcca ggtaccgctg aggccgaccg agtctctggc cactgtgggc
     8641 gcggcggggc ggcgatggtc gtccagcagg ccgacgacgg cgagctgatg gtgctccgcc
     8701 atgccgaccg aacgatggca atcctctaca cgcatctcat tcctgcaacg ttcggtggcc
     8761 gtgcgcgcat gaacgtggcc aatgccctgg cagcggcagc cgccgcctgg tcagtcgggg
     8821 cgcacctgca tgacatccgc cagggtctac gcacgttctc gacgtccttc ttccaggctc
     8881 caggtcgcct caacgagatg aacatcgacg gctacaaggt catcctcgac tactgccaca
     8941 acgtggatgg gatgcgccgt ctgacagagt tcgtcgactt gatggtcggc gcgaccaatg
     9001 acggagttaa gcgccccggc ggggtcagcg gccgcgcgat tggcgtgatc ggtatgcccg
     9061 gtgaccgtcg cgacgacgat caccgcgagt acgggcgctt ggcggcgcat tcgttcgacg
     9121 cggtcatcgt gcgcgaggat aagaacacgc gaggccgcga acgcggtcag tcggccgacc
     9181 tggtagctga gggggtccgt gccggcatgg ccgagggtgg ccgctgcagc gacgtgagga
     9241 tcgtcgtcaa cgagcaggag tcggcgatcg caggcctcca gctggcggag cgcgacgacc
     9301 tcgtggtcat ctgtgccgac gacatcacgg gtgtctaccg aaaagtgatg gagacggcca
     9361 agtcgaacgg cggcgccgcg ttcgccgacc ccggcgagtt ctcggctgag gagggctagg
     9421 ccctcactcc gggatcggga cttgcagcga cgacagcgtc ccgtcgccgg tattggtgac
     9481 ccataatcga tcctcggcga aaacgagacg acgcggctcg gcacccgcgg tatagcggtc
     9541 gagcacgtcg tctggcgaag caccagcaat gacggccact tcgccctggc cggcgacagc
     9601 gacccacagg ttgccggcct cgtcgaccat caaaccctca gagcccgccc cagtctggac
     9661 ggtcccgatc acctggttgg tctcagggtc gatctgagag acggtcccat cgcgtacgtt
     9721 cgcgacccag gcaatgtcag gccacaccat gtcctcggta aaggcgatgc cctccggccc
     9781 aaagccgccc tcgatcgtgg ctacgacctc attcgttgcg gggtcgaccc gcgtgatcgt
     9841 ggccgagccg aagttcgtga cccataccga tccgaaaccc aactcgacgt ggcgtggctc
     9901 gcggccgacc tcgaccgtgt cgaccacctc gttggtcagg gggtcgatgc ggtagaccgc
     9961 gccgtcctcc tcgcttgtga cccagatccc agcctcgtcg ccggcgatcc cctcaggttg
    10021 cgggccaacg tcgatcacgg cgatgacttc gtgtgtctcc ggattcaccc gcgcgacctc
    10081 ggccgattcg tgaacagata cccacaacga tcccgccgcc aaggtgatcc cgattgcccc
    10141 gataccgaca tgcacgcggt cgacgacctc gccgttcacc ggatcgaccg ctacgacgtc
    10201 accgtcgaag tagcacgcca cccagagcag gtcagccgcg gcggttatgc cgatcggact
    10261 tctgcagacg tcgccgacgc ccgggtcgga cgacggtgag gcagctggcg ccgccgtcgg
    10321 agcaggggtt ggagtcggcg tggccgagct tgttgttgct ggggtcgcgg aaaccgaggg
    10381 ttgaggtgtc ggattcgggc tcgcgcagcc cacgatcagg gccgccaggg cgaggaggcc
    10441 aagcgagcca cttcttcgag gcggcataat atgtggactc tagtcaatcg atggagaaat
    10501 gtccaccact gaacgcgacg ccacatctga tgagctgaag gcgctcggcc acccgctgcg
    10561 ctggcgaatc ctgcgagtaa cgctggatca cccgctgacc aacaaggaga ttgccgcgcg
    10621 gctcggccgg gacccgggta cgacgttgca ccacgtccgc gtcctcgctg acgccggctt
    10681 cctggcacct gagcgcgtcc gggcgggtgc caggggggct ctggaacggc cgtatcgcaa
    10741 cacaggcaag tcgtggcgga ttcggctcgc gccgcgtgcg gacaacactg ttgcgatcct
    10801 ggatgcggcg cgcgaggaga tcctggaggc cgggcccgac gcggcgatct cgaccctgcg
    10861 actggcggtg aatttgaagg cagccgacct cgaggagctc agtcggcgga tttcggacct
    10921 tggcgacgag ttcgcggggc gcggggatcc ggacggccag ccgctcggga tcgtggcgct
    10981 cgtccaccgc cggaggccct agggggcgat cgaaacgcgt cgagctctgc gatgtcagcc
    11041 acgggatgaa agtcagcccc ggacaacgcg tgcgccactt cctcccggtg ggcgtggcac
    11101 atcagcacgg tgactcgcgg ggcgccgctg gcgtcggcag ccatgcggcc tgcggtcgtg
    11161 agctcaccca ttagggccgt gacctcgtcg atgtagtcgg gcacctctgt ggcgttcagg
    11221 ccgccagcgg tcatgccagc gcgaagctcg cccagcgcac ttgctcgcga gcggccgcgc
    11281 aggaaaggca ggtcctcctt gatcgcc
//
LOCUS       DATLCO010000175         2540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5457057, whole genome shotgun sequence.
ACCESSION   DATLCO010000175 DATLCO010000000
VERSION     DATLCO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5457057"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1569)
                     /locus_tag="VMZ33_04425"
     CDS             complement(13..1569)
                     /locus_tag="VMZ33_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16505.1"
                     /translation="MTLLGLMLPAAVSAHPLGNFTINRYNGLTVAPTWTHLDRVVDMA
                     ELPTVQARQEMDINRDGAVADEEAAAWATSACAAAPSSLTFSIGSKAAELLPAGVGLT
                     FAPGQAGLQTLRLACTYVSEYSAIASAETLTFDDASYPNRVGWREIVIAGSATETGGA
                     EAFAAGSSDRLHTYPADASGATRAQTSARFSVKPIDGATAAQPIVDEAVPLGSPADAI
                     VHTSTQTAAVPGGVSDLPAELGAILQAGDLSLPAMVLAFLVAGLVGAFHAVTPGHGKT
                     LMAAYLVGTRGTSRHALALGLTVTVSHTIGVLALGAIVLVADAALPSERLLPILGLAS
                     GILVTLLGSVFLFQRVRTALHEREHVREQEREHARQHAQGHDHAHDHAHAHDRGQGQE
                     DAGWHSHGLVRHTHLPSTDGPLRTRNLIALGLVGGLVPSASAILILVGSIAAGRPALG
                     MALTVAFGAGMALVLVGVGVMLVRARSLVERIPSARLGRALAYAPIVTAITFLVVGVA
                     ITVQAGGQLR"
     gene            complement(1755..>2540)
                     /locus_tag="VMZ33_04430"
     CDS             complement(1755..>2540)
                     /locus_tag="VMZ33_04430"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HUR16506.1"
                     /translation="SSFNVNTIAIQLPITDLTNDGEAVTAADATNAVVGIWAGAARKS
                     TTVLNAAGVPDISGDYVQVSRLGNPLINEVIIPLGEKDEWNASQPADDANYMDRYLNS
                     ELAAIVNTVYPSLPDARTTDRSDLVLILGQGVPGLNATNTGDTLYDMLRLNMGIAPSA
                     NPSRLGVLDGDLAGFPNGRRVWDDVVDIEIRAVADGYGTFLEENFGLPNETPNNMVGD
                     GCDANDKAFLGSFPYVAGPWQGYSDGVHHLSPCEALDETSTAQ"
BASE COUNT          428 a          778 c          912 g          422 t
ORIGIN      
        1 tggccaagct catcatcgga gctggcctcc cgcctggacg gtgatggcga cgccgaccac
       61 caggaacgtg atcgccgtga cgatcggcgc gtaggccaga gctcggccca agcgggcgct
      121 ggggattcgc tcgacgagag agcgggcacg gaccagcatc acgccgaccc ccaccagcac
      181 cagcgccatg ccggcgccga aggcgacagt caaggccatc ccgagtgccg ggcggcctgc
      241 ggcgatggag ccgaccagga tgaggatggc cgaggccgac ggcacgagac ctccgaccag
      301 tccgagggca atcaggttgc gagtgcgcag cgggccgtcc gttgacggca ggtgggtatg
      361 gcggaccaga ccgtgggagt gccagcccgc gtcctcctgg ccctgccctc gatcgtgagc
      421 gtgcgcgtgg tcatgcgcgt gatcgtgtcc ctgcgcgtgt tgtcgggcgt gctcacgctc
      481 ctgctcgcgg acgtgctcac gctcgtggag cgcggtgcga acccgctgaa agaggaagac
      541 ggagccgagc agcgtgacga gaatgccgga cgcgagcccg aggatgggca gcagccgctc
      601 cgacgggagt gctgcatcgg ctacgaggac tatcgcgccc agcgcgagga cgccgatggt
      661 atgggagacg gtcaccgtca agccgagagc gagggcatgg cgcgatgtgc cgcgcgtgcc
      721 aacgagatag gcggccatca aggttttacc gtggccgggc gtgacggcat gaaatgcgcc
      781 gaccaggcct gcgaccagga acgccagcac catcgcgggc aggctcaggt cgccggcttg
      841 aaggattgcg ccgagctcgg caggcagatc ggatacaccg cccggaacgg ccgcggtctg
      901 cgtgctcgtg tgcacgattg catcggcggg gcttcccagc ggcaccgcct catcgacgat
      961 cggctgagcg gccgtggcac cgtcgatcgg cttgacgcta aagcgcgcag aagtctgtgc
     1021 gcgggtggct cccgacgcgt cagctgggta ggtgtgcaag cgatcagagg atccggccgc
     1081 gaacgcctcg gccccgccag tctccgtcgc gctcccggca atgacgatct cgcgccaacc
     1141 gacgcgattg gggtatgaag cgtcatcaaa ggtaagggtc tcggccgacg cgatggcgga
     1201 gtactcgctg acataagtgc acgccaggcg aagggtctgg aggcctgctt gtcccggggc
     1261 gaatgtcagg ccaacacccg ccgggagcag ttcggcggcc ttgctgccaa tgctgaacgt
     1321 cagcgagctc ggggcggcgg cgcaggctga tgttgcccag gccgctgcct cttcatcggc
     1381 cactgcgcca tcacggttga tgtccatctc ctggcgtgcc tgcaccgtcg gcagctcggc
     1441 catatccacg acgcgatcaa gatgtgtcca ggtcggcgcg acagtcagtc cgttgtagcg
     1501 attgatcgtg aagttgccca gggggtgggc gctcacggcc gcggggagca tcaggccgag
     1561 cagcgtcatc gccagcgtgg cgagagcgct caggcgaacg cggcgtgcgg tgggggatgc
     1621 agactttcga gttccagtcg agagtgccat tcggttgctg tgccttctgt gtgctgatgg
     1681 gccgggcgcc gcgtgggata cgcggcgccc ggttgccatc gaggggtcag tccctggaag
     1741 tcgctagaag cggcttactg ggctgtgctt gtctcgtcga gcgcttcgca aggcgagagg
     1801 tgatgtacgc cgtccgagta gccctgccaa gggccagcca cgtacgggaa cgaaccaagg
     1861 aacgccttgt cgttcgcgtc gcagccgtca ccgaccatgt tgttcggtgt ctcgttcggg
     1921 agtccgaagt tctcctcgag gaacgtgccg tagccgtcgg ctactgcgcg gatctcgata
     1981 tcgacaacgt cgtcccagac gcggcgcccg ttggggaagc cggccaggtc gccgtcgagt
     2041 actccgaggc ggctgggatt ggcgctgggc gcgatgccca tgttcagccg cagcatgtcg
     2101 tacagcgtgt caccggtgtt ggtggcattc agtccgggta caccctggcc gaggatcagc
     2161 accaggtcgc tccggtcggt tgtccgagca tcgggcagcg acggatagac ggtgttgacg
     2221 atggctgcca actcagagtt gaggtagcgg tccatgtagt tggcgtcgtc agcaggctgc
     2281 gacgcgttcc actcatcctt ctcgccgagc gggatgatga cctcgttgat aagcgggttg
     2341 cccaggcgcg aaacctgaac gtagtcgccg gagatgtccg ggacgcccgc ggcattgaga
     2401 acggtggtgg acttgcgcgc ggcgcctgcc caaatgccaa cgaccgcgtt ggtcgcgtca
     2461 gcggccgtaa cggcctcgcc atcgttggtg agatccgtga tcggcagctg gatggcgatg
     2521 gtgttcacgt tgaacgagct
//
LOCUS       DATLCO010000176         3921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3964523, whole genome shotgun sequence.
ACCESSION   DATLCO010000176 DATLCO010000000
VERSION     DATLCO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3964523"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VMZ33_04435"
     CDS             complement(<1..240)
                     /locus_tag="VMZ33_04435"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HUR16507.1"
                     /translation="MPMYLTRFSYTPEAWARLTKNPEDRRNAASTVIESVGGKLHGFW
                     YAFGEYDGYTLWEAPDNGSMAAVAIALGSGGSLSKV"
     gene            complement(292..2076)
                     /locus_tag="VMZ33_04440"
     CDS             complement(292..2076)
                     /locus_tag="VMZ33_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16508.1"
                     /translation="MNGTRLGALASALLLVAACGATVTTPTPATTSGPGASPTTAPAA
                     STDGPIVEPTPTGEATPAPPIATPEPQPLPKPELHTIDDITAVLYERAQAELAIVSIL
                     DLLDIGLYAADGSVVRSGSESSDADYFVYEDEARGLATLLRSYDVEDRWVSFADFHAA
                     LVDLGLQGTPEQLAAAYNSAYEEDFESPIVQLVLALGPIDPESDLHPLAQWLLFLDGF
                     VPPHGDGHGAAGGVASNHTPRWGVARDGVQALVPVPLEADPLVIAHLMSVVGSASINV
                     QANPPKAHEGHGGPGSPTAITGTVTAIASTFISPFSERPLIPLAPTAGAGLPVTWHPD
                     ATLNEHGTGPIPPHGVTDPLGRASFDFTPRQEDANGEGYLTSEVSLIQASVSGAQLVT
                     QLYGVPSLGALVGREVRGLGTLEIEWHAAEAMRISLVNTYDVTLELIIGRATGDGTDS
                     FNGILALQEDGSWRGVVDGTATGTYRGTALGEACTTSWSARQLVEVIGQASPHALNGD
                     FYFRFEPATPPTGSLGSGPCKSSLASYEGIDYAPYNDASITNAAQDQGLVIILPQKPG
                     GTHDYPVKLEGVVDANWHVEIEFLEPQP"
     gene            complement(2073..>3921)
                     /locus_tag="VMZ33_04445"
     CDS             complement(2073..>3921)
                     /locus_tag="VMZ33_04445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUR16509.1"
                     /translation="TATDKATWLVAHLLDWHRREDKSTYWRFFDLMSKSDEELIEEGE
                     PIGGLELTDEWELPKPSKSKIYRYTFPAQDFKIDVGHSLNDPRLYAAGEKRTGTGSVH
                     AVDDTALTLDIKRGRDWAGPHPTSVVGLDVIDAKAQRTAIMRLAEWVVTNGMESEAAE
                     WHAARDLLLRQAPRLSGRSGGKLVSDDRSGSEAAVEIALQLNGTTLAIQGPPGSGKTY
                     TGARMIWQLIDKERVVGVTSNSHKVIGNLVKAAIDTVPEGKSIRIVQKAKAHEAVEHP
                     WVKRVDANEDVDEAIAEGSVDLVAGTPWLWAREPMHGRVDTLFVDEAGQVSLANVVSI
                     SGAARNVVLLGDPQQLDQPTQGVHPDGSGKSALGHFLGEAKVVPEDRGLFLEKTWRMH
                     PQITRYTSELFYEDKLHSIDGLERQRIEGAGWLAGSGLRWAGVDHDGNTNESLEEALA
                     VVDIVRELLGRNWTNKDGVQQAIVKDEIRVLTPYNAHRLLIDDQLAKAGISGVQVGTV
                     DKFQGQEAAVAIYTMATSRPEDAPRGMDFLYSLHRLNVATSRAKALAIVVASPLLLRA
                     VPQTPEQLRMANGLAAFVEHALPRTEEVPVPPESSDLAETTPAQLALPL"
BASE COUNT          600 a         1294 c         1330 g          697 t
ORIGIN      
        1 caccttggag agcgaaccgc cgctgccgag ggcgattgca accgccgcca ttgagccgtt
       61 gtcgggcgcc tcccagaggg tgtagccgtc gtactcgccg aaggcatacc agaagccgtg
      121 gagcttgccg ccgactgatt caatgacggt gctcgcggcg ttccgacggt cctcggggtt
      181 ctttgtcagg cgtgcccagg cctcgggcgt gtagctgaac cgggttaggt acatcggcat
      241 ggttctcctc cttgtggctc ggctgcgggc cgccgacgcc agaagcgcat gctacggctg
      301 cggctccaga aattcgatct cgacgtgcca gttggcatcg accacgccct ccagcttgac
      361 cgggtaatcg tgggtgccgc ccggcttctg cggaaggatg atcaccaggc cctggtcctg
      421 cgcggcattg gtgatgctgg cgtcgttgta cggcgcgtag tcgatgccct cgtagctcgc
      481 caggctcgac ttgcaggggc cactgccgag cgagcctgtc ggcggggtgg ctggttcgaa
      541 gcggaagtag aagtcaccgt tgagcgcgtg cggcgacgcc tggccgatga cctccaccag
      601 ctgcctcgct gaccagctcg ttgtgcacgc ctcgccgagg gccgtgccgc gataggtgcc
      661 ggtcgcggtg ccgtcgacga cgccacgcca cgagccgtcc tcctggaggg cgaggatgcc
      721 gttgaacgag tcggtgccat cgccggtggc tcgaccgatg atcagctcca gggtcacgtc
      781 gtacgtgttg accagcgaga tgcgcatcgc ctcggctgcg tgccactcga tctccagcgt
      841 gcccagcccg cggacctcgc gtccgacgag cgctcccagg gagggcactc cgtacagctg
      901 cgtcaccagc tgtgcgcccg acactgaagc ctgaatgagc gacacctcgc tggtcaggta
      961 gccctcgccg ttcgcgtcct cctgcctcgg cgtgaagtcg aagctcgcgc ggcccagcgg
     1021 atccgtgact ccgtgtggcg gaatgggccc cgtgccgtgc tcgttgagcg tggcgtcagg
     1081 gtgccacgtg accggcagtc cggcgccagc cgtcggggcg agcggaatga gcggccgctc
     1141 gctgaatggt gagatgaatg tgctggcgat cgcagtgacc gtgccggtga ttgcagttgg
     1201 cgagcccggt ccgccgtgcc cctcgtgtgc cttcggcgga ttggcctgga cgttgatgga
     1261 cgccgatccc acgaccgaca tcaggtgggc gatgaccagc gggtcggcct cgagtggaac
     1321 cgggacgagc gcctgcacac cgtcgcgcgc cacgccccag cgcggcgtgt ggttcgacgc
     1381 gacgccaccg gcggcgccgt ggccgtcgcc gtggggaggc acgaagccgt cgagaaacag
     1441 gagccactgc gcgagtgggt ggaggtccga ctcgggatcg atcgggccga gcgcaaggac
     1501 gagctggacg atcggggact cgaagtcctc ctcgtaggcc gagttgtagg cagccgccag
     1561 ttgctcaggt gtcccctgca acccgaggtc gaccagggcg gcatggaagt cggcaaagct
     1621 gacccatcga tcctcgacgt cgtacgacct cagcagggtg gccagcccgc gggcttcgtc
     1681 ctcgtagacg aagtagtcgg catccgacga ctccgatcca cttcgaacga ccgatccgtc
     1741 ggcggcgtac aggccgatat cgagcaggtc gaggatgctg acgatcgcca gctcggcctg
     1801 ggctcgctcg tagagaaccg ccgtgatgtc gtcgatggtg tgcagctcag gcttcggcag
     1861 cggttgcggt tcgggcgtcg ctatcggcgg cgccggtgtc gcctcgcccg ttggcgtcgg
     1921 ctctacgatc ggcccgtcgg tcgaggcagc tggcgctgtc gtcggcgagg cgcctggacc
     1981 ggaagtggtg gcgggcgtcg gcgtcgttac cgttgcgccg caagcggcca cgagcagcag
     2041 cgcggaggcg agggcgccga gccgcgtccc gttcacagcg gcaaggccag ctgggccggt
     2101 gtggtctcgg caagatcgct cgactccggc gggacaggca cctcctcagt gcgcggcaga
     2161 gcatgctcca caaaagcggc cagtccatta gccatccgca gctgctccgg tgtctggggt
     2221 acggcccgca gcaggagggg tgacgcaacg acgatcgcga gtgccttggc gcgcgaggtg
     2281 gcgacgttga gccgatgcag cgaatagagg aagtccatgc cgcgtggcgc atcctcgggc
     2341 cgcgacgtgg ccatggtgta gatggcgacc gccgcctcct gcccctggaa cttgtcgacg
     2401 gtgcccacct gaacaccgga aataccagcc ttggccagct gatcgtcgat caacagccga
     2461 tgggcgttgt aaggcgtcag cacgcggatc tcgtccttga cgattgcctg ctgcactccg
     2521 tccttgttgg tccagttgcg gccgagcagc tccctgacga tgtcgacgac ggcgagcgcc
     2581 tcctcgagcg actcattggt gttgccgtcg tggtccaccc ctgcccaacg cagtccagag
     2641 cccgccagcc agccggcacc ctcgattcgc tgtcgctcga ggccatcgat cgagtgcagc
     2701 ttgtcctcgt agaacagctc ggacgtgtag cgggtgatct gtgggtgcat gcgccacgtc
     2761 ttctcaagaa agaggccccg atcttctggc acgaccttgg cttcgcccag gaagtggccc
     2821 aacgcggact tgcctgagcc atcgggatgg acaccctgcg tgggctggtc cagctgctgc
     2881 ggatcgccga gaagcacgac gttgcgcgcg gcaccactga tggacaccac gttcgccagc
     2941 gagacctgcc cggcttcgtc cacaaagagc gtgtcgaccc gtccgtgcat gggttcgcgc
     3001 gcccacagcc acggcgttcc ggccacgagg tcgaccgaac cctcggcaat cgcctcgtct
     3061 acgtcctcgt tcgcgtccac ccgcttgacc catggatgtt cgaccgcctc gtgcgccttg
     3121 gccttctgca cgattcggat tgacttgccc tcaggaaccg tgtcgatggc agccttgacc
     3181 aggttgccga tgaccttgtg gctgttcgac gtgaccccga cgacgcgctc cttgtcaatg
     3241 agctgccaga tcatccgcgc gccggtgtag gtcttgcccg agccgggcgg gccctggatc
     3301 gcgagcgtcg ttccattcag ctggagagcg atctcgacgg cggcttccga ccccgacctg
     3361 tcatcggaca ccagctttcc gcccgatcga ccagacaggc gcggagcttg ccgaagaagc
     3421 aggtcgcgcg ccgcatgcca ctcggccgcc tcagactcca tgccgttggt gacgacccat
     3481 tcggccaggc gcatgatcgc cgtgcgctgg gccttggcgt ctatcacgtc gaggcccacg
     3541 acggacgtcg gatgcggacc ggcccagtcg cggccgcgct tgatgtcgag cgtcagggcc
     3601 gtgtcatcga cggcgtgcac cgagccggtc ccggtcctct tctcgccggc ggcatacagc
     3661 cgcgggtcgt tcaggctgtg gccgacgtcg atcttgaagt cctgcgccgg aaaggtgtat
     3721 cggtagatct tcgatttgga tggtttgggc agctcccact cgtcagtgag ctccagcccg
     3781 ccgatcggct cgccctcctc gatcagctct tcgtccgact tgctcatgag gtcgaagaag
     3841 cgccagtagg tcgacttgtc ctcgcgccgg tgccagtcca gcaggtgagc aaccagccag
     3901 gtggctttgt cggtggcggt c
//
LOCUS       DATLCO010000177         3385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7573763, whole genome shotgun sequence.
ACCESSION   DATLCO010000177 DATLCO010000000
VERSION     DATLCO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7573763"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VMZ33_04450"
     CDS             complement(<1..232)
                     /locus_tag="VMZ33_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HUR16510.1"
                     /translation="MEYRDYYATLGVPRTASQADIKKQFRKLARQHHPDVNRGDSEAE
                     RKFKEVSEANEVLSDPDKRKLYDQLGSNWQAYQ"
     gene            complement(326..2854)
                     /gene="lon"
                     /locus_tag="VMZ33_04455"
     CDS             complement(326..2854)
                     /gene="lon"
                     /locus_tag="VMZ33_04455"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HUR16511.1"
                     /translation="MTDTNPTDEQVEEPQDSGNQDGGATRELPLVALRETVIFPEMIV
                     PLQVGRDKSVQALNKAVADGTPIALVTQRQADREDINTPAELYEVGTLAKIAQVVQLQ
                     DGTVRAIVQGQQRLRLLEFVDSEPFILARIELVEEEPASGLEVQALVRSVQGQIEQYV
                     QSGAPVPPEAAVAARNITEPGLLADMVAYSPDMTTEQRQELLETIDVHERLKLVSSFL
                     GRQIEILDLKGKIQSEVKSEMDKTQREYILREQLKAIQRELGEDDPQQAEINELREKV
                     EAAGMPEEIKARAVKEVDRMSRIPSASPEVGVIRTYVDWLIGLPWDVSTDDNLDIKEA
                     ARILDEDHAGLEKIKERILEYLAVRTLAEKIRSPILAFVGPPGVGKTSLGKSVAKAMG
                     RKFVRMSLGGIHDEAEIRGHRRTYIGALPGRVIQNIKTAGSNNPVFMLDEVDKIGMDF
                     RGDPSSALLEVLDPEQNNTFQDNYLEVPFDLSKVLFIATGNLLDPIPPALRDRMEIIQ
                     LPGYTQQEKSEIGKRFLIPKQLENHGLTTKNVDFTDEAMTELVQAYTKEAGVRNLERE
                     IANVMRKIARQVAEGRKRKVTVNAKKLSEYLGPPRFEYGELEKENQIGSATGLVVTEV
                     GGDVVAVEVTRMPGKEDFILTGQLGEVMRESARAGLSWIRAHADELGIERDAFEKYTL
                     HIHVPAGGIPKDGPSAGITMALAMVSAFTGIAVKKDVAMTGEITLRGRVLPIGGLKSK
                     ILAAHLSGSGAVILPLKNQKDLIDIPQEIRKQVKLVLVESMDQVLEHALIRVPKRLEA
                     KPAEVVEKGEKIDEESLAAPDKILRGFPGDQPPAIAQSTTHVLS"
     gene            complement(2882..3214)
                     /locus_tag="VMZ33_04460"
     CDS             complement(2882..3214)
                     /locus_tag="VMZ33_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUR16512.1"
                     /translation="MYVISVAAELVRVHPRTLRIYELEGLVCPARTRSNIRLYSENDI
                     RRVLWIRHLTRNLGVNLAGVRVLFELEERLGMRILETLYEDRTTGARAHLEQEELAAS
                     QAESAANG"
BASE COUNT          494 a         1092 c         1090 g          709 t
ORIGIN      
        1 gctggtaggc ctgccagttc gaaccgagct ggtcgtagag cttccgcttg tcggggtcgg
       61 agaggacctc gttggcctcg ctgacctcct tgaacttgcg ctcagcctcg gagtcgcccc
      121 tgttgacgtc cggatggtgc tggcgggcca gcttgcggaa ctgtttcttg atgtcggcct
      181 gactcgccgt ccgcggtacg ccaagcgtgg cgtagtaatc gcggtactcc aactacttca
      241 cctccgcagt cggtctgcgg accgctgctg cgcgcccacg gtgaacttcg ctcgcgcgga
      301 gttccacctg cggccggcgg tcgacctagc tgagaacgtg ggttgtggat tgggcgatgg
      361 ccggcggctg gtcgccgggg aagccgcgca ggatcttgtc gggcgcagcg agcgactcct
      421 cgtcgatctt ctcgcccttc tcgaccactt ctgccggctt ggcttccagg cgcttgggca
      481 cgcggatcag cgcgtgctcg agcacctggt ccatcgactc gaccaggacc agcttgacct
      541 gcttgcggat ctcctgcggg atgtcgatca gatccttctg attcttcagc ggcaggatca
      601 ctgcgccgga accggaaaga tgcgcggcga ggatcttgct cttgaggcct ccgatcggca
      661 gcacgcgacc acgcaacgtg atctcgcccg tcatcgccac gtccttcttg acggcgattc
      721 cggtgaaggc gctgaccatg gccagggcca tcgtgatacc cgcggacggt ccgtccttgg
      781 ggataccgcc ggccggtaca tggatgtgca gcgtgtactt ctcgaaggcg tcacgttcga
      841 tgcccagctc gtcggcatgt gcgcggatcc acgagagccc ggcacgtgcc gattcgcgca
      901 tgacctcgcc cagctgcccg gtgaggatga agtcctcctt gcccggcatg cgggtgacct
      961 cgaccgcaac cacatcgccg ccgacttcag tcacgacgag acccgtcgca gaaccgatct
     1021 ggttctcctt ctcaagctcg ccgtactcga agcgcggcgg gccgaggtat tcactcaact
     1081 tcttggcgtt gacggtcacc ttgcgcttgc gcccctcagc cacctgacga gcgatcttgc
     1141 gcatcacgtt ggcaatttcg cgctccagat tgcggacgcc cgcttccttc gtataggcct
     1201 gaacgagctc tgtcatggcc tcatcggtga agtcgacgtt cttcgtcgtc aaaccgtggt
     1261 tctcgagctg cttcgggatc aggaaacgct tcccgatctc gcttttctcc tgctgggtat
     1321 agcccggcag ctggatgatc tccatgcggt cgcggagcgc cggcgggatg ggatcgagca
     1381 ggttgccggt cgcgatgaag agcaccttgg agaggtcgaa cggcacctcg aggtagttgt
     1441 cctggaaagt gttgttctgc tccgggtcga gcacctcgag cagggccgag gatggatcgc
     1501 cgcggaagtc catgccgatc ttgtcgacct cgtcgagcat gaagaccggg ttgttgctgc
     1561 cggcggtctt gatgttctgg atgacgcggc ctggcagggc gccgatgtac gtgcgtctgt
     1621 gaccgcggat ctcagcctcg tcgtggatac cacccaggct catgcgcacg aacttgcggc
     1681 ccatggcctt ggcgacactc ttgccgagcg aagtcttgcc cacgcctggc ggtcccacga
     1741 acgccaggat ggggctgcga atcttctccg cgagggtgcg caccgcgagg tactcgagga
     1801 tgcgctcctt gatcttctcg agaccggcgt gatcctcgtc gaggatcctg gcggcttcct
     1861 tgatgtccag gttgtcgtcg gtcgagacgt cccacggcag gccgatcaac cagtcgacat
     1921 acgtccggat gacgcccact tcgggcgacg ccgacgggat gcgactcatc cggtcgactt
     1981 ccttgacggc gcgggccttg atctcctccg gcatgccggc ggcctcaacc ttctcgcgca
     2041 gctcgttgat ctcagcctgc tgcggatcgt cctccccgag ctcgcgctgg atcgccttca
     2101 gctgctcacg caggatgtac tcgcgctggg tcttgtccat ctcggacttg acctctgact
     2161 ggatcttgcc cttcaggtcg agtatctcga tctggcggcc gaggaacgat gagacgagct
     2221 tcagccgctc gtggacgtcg atcgtctcga gcagctcctg gcgctgctcg gtggtcatgt
     2281 ccgggctgta ggcgaccatg tcggccagca ggcccggctc ggtgatgttg cgcgcggcga
     2341 cggccgcctc cggcgggacc ggcgccccgg actggacgta ttgctcgatc tggccctgca
     2401 cgctgcgcac gagagcctgg acctccagtc cgctggcggg ctcttcctcg acgagctcga
     2461 tgcgggccag gatgaagggc tccgagtcaa cgaattccag gagtcgcaga cgctgctggc
     2521 cctgcacgat ggcccgcacc gtgccgtcct ggagttggac gacctgggca atctttgcca
     2581 gcgtcccgac ctcgtacagc tcagccgggg tattgatgtc ctcccggtcg gcctggcgct
     2641 gggttaccag cgcgatcggc gtgccgtcgg caaccgcctt gttgagtgcc tgaaccgact
     2701 tgtcgcggcc gacctgaagc ggcacgatca tctccgggaa gatgaccgtc tcgcgcaggg
     2761 ccacgagggg cagctcgcgc gttgcgcccc catcctggtt gcctgaatcc tgcggctcct
     2821 cgacctgctc gtcagttggg ttggtgtctg tcatttcgct cctaagggtt tcgcagggtc
     2881 gctagccgtt ggccgcggat tcggcctggc tggctgccag ttcttcctgc tccaggtggg
     2941 ccctggcgcc ggtcgttctg tcttcgtaga gggtttcgag gatgcgcatt ccgaggcgct
     3001 cctcaagttc gaacaggacg cgaacgccgg cgaggtttac gccgaggttg cgcgtgaggt
     3061 gccgaatcca gaggactcgc cgaatgtcgt tctctgagta cagacggatg ttgctgcgcg
     3121 ttcgtgccgg gcagacgagg ccttccagct catagatgcg caaagttcgg ggatgcacgc
     3181 gtaccagctc tgcggcgacg ctgatgacgt acaccgggcg agtagggtcc cggcgatcgg
     3241 caccaactgc cgtatccgtc tcacggctgc gcgtgtctgt catgtgtcag ctggcctgtt
     3301 cgacgcgcgg ctggccgttg ccctgaccgt cggcctgcca ttggtcgccg gcctgccact
     3361 cactggtgga ctgccactcc gtggc
//
LOCUS       DATLCO010000178         3642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3966506, whole genome shotgun sequence.
ACCESSION   DATLCO010000178 DATLCO010000000
VERSION     DATLCO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3966506"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VMZ33_04465"
     CDS             <1..414
                     /locus_tag="VMZ33_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16513.1"
                     /translation="EDELRVYLPLIAAGDPQTLEQIDAIWYLRGKATFLFEVEWTAML
                     TDALLRRGPRIPSDETIVRFLVVPPERIELMRLKLARSPLLRKALEEQNWHILKSNHL
                     RRLHGRDTADLDDLAPLLGLDPDIEREAEQLPLFG"
     gene            436..2112
                     /locus_tag="VMZ33_04470"
     CDS             436..2112
                     /locus_tag="VMZ33_04470"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUR16514.1"
                     /translation="MTDVALGSAVTDLADRFWQWFLVRAPMYATVLGDERYDDRLPDP
                     TAEGRATEVAGLKGFLGDAHGIERSSLDQEDSITLDMLEVVARIWLRQHEHDVHHFDA
                     MDQMGGPQNLPGDMSRFQRLDTPERLERAIVRLESFPHYLAAHRANLLDGIAAGRTAA
                     RPVVARVLDQVRRAVNTPVDENPLLIAHPELDETSRDRLRHVIEHSVQPALADHLAAI
                     EGYAEFAREREGVWALPDGEALYATQIVASTTLEESPQALHDYGLEQIEKIRGEVAEI
                     AKELGFADAKSMREALAKDPNNYATDGADIVARAERQIQRANDVAPRYFGRLPIAGCE
                     VRPVEPYQEAEAPPAFYFPPAPDGSRGGIYYVNTYQPQNRPLYRLATTTYHEATPGHH
                     FQIAMEGELTDMPDFRRFGARLTGAAYAEGWGLYSERMADEMGLFESAWERLGMLDAQ
                     AWRAARLVVDTGLHAFQWPRQKSIDLLLDIGLSQLEAETETDRYITWPGQALAYMTGQ
                     REIMALRHQLEARDGDRFDLVKFHDELLGHGTLPLATLRKHLPGWVTPRF"
     gene            2188..3138
                     /locus_tag="VMZ33_04475"
     CDS             2188..3138
                     /locus_tag="VMZ33_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HUR16515.1"
                     /translation="MDIQRLINDLAASGALRVIIQIALIVLATLLAMRFAHVLVSGTL
                     NRLFQREVAEGTAQELSAVELERRRQTLDHLAYRSLRVIILVIAFLMVLHVLDLNIGP
                     AIAGLGVVGLSLSLGAQHLVRDYVAGAFILIENQYSKGDVVTIAAVSGTVEDVSLRRT
                     TLRDHDGTVHYVPHGLIQTASNHTRNWAAIEIDLPITYEQDLAALTEAVAAAGKRLAT
                     DPKWRAAIFEAPRVASVERLAETGLLVRVSASVAALHRYALPGVARGLVLDECTRRGI
                     VIGWREAPTALADSPARPAKADLSSEAAAPLAMPPTEADG"
     gene            3148..>3642
                     /locus_tag="VMZ33_04480"
     CDS             3148..>3642
                     /locus_tag="VMZ33_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HUR16516.1"
                     /translation="MTDTSLPGSLSEPELENLLAENRRFPPAADFMAQANATADLYEE
                     AERDPEAFWARLARERLAWDTPFETTLEWEPPFAKWFLGGKINASYNCVDRHVENGLG
                     DKVAYHWIGEPGDTRTITYADLQREVSKAANALKELGVKSGDVVAIYMPMIPELPIAM
                     LACAR"
BASE COUNT          617 a         1251 c         1184 g          590 t
ORIGIN      
        1 gaggatgagc tccgtgtcta cctgccgctc atcgcagccg gcgacccgca gacgctggag
       61 cagatcgacg cgatctggta cctgcgtggc aaggcgacgt tcctgttcga ggtcgagtgg
      121 acggcgatgc tcaccgacgc gctcctgcgc cgcggaccca ggatcccgag cgacgagacg
      181 atcgtccgct tcctcgtcgt gccgcctgag cggatcgagc tcatgcgcct gaagctggct
      241 cggtcgccgc tactccgaaa agccctcgag gagcagaact ggcatatcct caaatccaat
      301 cacctgcgcc gattgcacgg ccgggacacg gccgatcttg acgatctggc gcctctcctc
      361 ggactggacc cggacatcga acgagaagcg gaacagctgc cgctcttcgg ctgactcacc
      421 tctggaggat cacccgtgac cgacgtagcg ttgggaagcg ccgtcaccga ccttgcagat
      481 cgcttctggc aatggttcct tgttcgggcg cccatgtacg caacggtgct cggcgacgag
      541 cgctacgacg accgcctgcc cgatccgaca gctgagggtc gcgccacgga ggtcgccggc
      601 ctcaagggct tccttgggga cgcccacggg atcgagcgct cgagtctcga tcaggaagac
      661 tccatcaccc tcgacatgct tgaggtggtg gctcggatct ggttgcgcca gcacgagcac
      721 gatgtccatc acttcgacgc catggaccag atgggcggcc cccagaacct gcccggcgac
      781 atgtcgcgct tccagcgcct cgatacgccg gagcggctcg agcgggccat cgtccggctc
      841 gaatcgttcc cgcactacct ggcggctcat cgggcgaacc tgcttgacgg cattgcggcc
      901 ggtcgcaccg cagccaggcc ggtcgtggcc cgcgtccttg accaggttcg ccgcgccgtg
      961 aacacgccgg tcgacgaaaa ccccttgctc atcgcccatc cggagttgga cgagacctcg
     1021 cgcgaccggc tacgccacgt gatcgagcac tcggttcagc cggcgctggc cgatcacctc
     1081 gcggcgatcg agggctatgc cgagttcgcg cgtgagcgcg agggcgtctg ggcactgccc
     1141 gacggcgagg cgctgtatgc gacacagatc gtcgcttcga cgacgctcga ggagtccccg
     1201 caggcactcc acgactacgg tctggagcag atcgagaaga tccgtggcga ggtcgccgag
     1261 atcgcgaagg agctcggctt tgccgatgcg aagtccatgc gcgaggctct ggccaaggac
     1321 ccgaacaact acgcgacgga cggcgcggac atcgtggcgc gcgccgagcg acagatacag
     1381 cgtgccaatg acgtggcgcc gcgctacttc ggtcgactgc cgatcgccgg ctgcgaggtc
     1441 cgcccggtcg agccctacca ggaggcggag gcaccgcccg ccttctactt cccgcccgcg
     1501 cccgacgggt cgcggggcgg catctactac gtcaacacgt accagcccca gaaccgccct
     1561 ctctaccgac tggccacgac gacgtatcac gaagcgaccc cgggtcatca cttccagatc
     1621 gccatggagg gcgagctgac cgatatgccc gatttccggc gcttcggcgc tcgcctcacc
     1681 ggcgccgcgt acgccgaggg ctgggggctc tacagcgagc gcatggcgga cgagatgggc
     1741 ctattcgaga gcgcctggga gcgactgggc atgctcgacg cgcaggcgtg gcgcgccgcc
     1801 cgactcgtcg tcgacactgg gctgcacgct ttccaatggc cccgccagaa gtcgatcgac
     1861 ctgctcctcg acatcggcct gtcgcagctg gaggcggaga cagagaccga ccgctacatc
     1921 acgtggcccg gtcaggccct cgcctacatg accggccagc gcgagatcat ggcgcttcgt
     1981 caccagctcg aggcgcgcga cggcgatcgc ttcgacctgg tgaagttcca cgatgagctc
     2041 ctcggccacg ggacgttgcc gctggctacg ctgcgcaagc acctgcctgg ctgggtcacg
     2101 ccccgcttct gaccgcagct cggctgccgc ggccgaagtt acggatcgac gcccgtcagc
     2161 gcccggtcgc tcgataacct agaggcggtg gacattcaga ggctgatcaa cgacctggct
     2221 gcctccggcg cactgcgcgt gatcatccag attgcgctga tcgtcctggc gacgttgctg
     2281 gcgatgcgct ttgcgcacgt gctcgtgtca ggcacgttga accggctctt ccaacgcgaa
     2341 gtggctgagg ggacggctca ggagctgagc gccgtcgagc tagagcggcg tcggcagacg
     2401 ctcgaccacc tcgcctaccg gtcgctgcgc gtgatcatcc tggtgattgc cttcctgatg
     2461 gtgctgcatg tgctcgacct caatatcgga ccggcgatcg ccggacttgg cgtggtcggg
     2521 ctgtcgttga gcctgggcgc gcagcacctc gtgcgcgatt acgtggccgg agcgttcatc
     2581 ctcatcgaga accagtattc gaaaggtgat gtcgtgacga ttgccgcggt aagcggcacg
     2641 gttgaggacg tgagtctgcg gcgcactacc ctgcgcgatc acgacgggac ggtccactac
     2701 gttcctcacg gcctcatcca gacggcgagc aaccacacgc gcaactgggc agcgatcgag
     2761 atcgatctgc cgatcaccta cgaacaggat cttgcggcgc tgaccgaggc ggtcgccgca
     2821 gctggcaagc gcctggcgac agaccccaag tggcgcgccg cgatcttcga ggcgccgcgc
     2881 gtcgcgagcg tcgagcggct ggccgagacg ggccttctgg ttcgggtatc ggcatcggtc
     2941 gcggctctgc accggtacgc gctccccgga gtagcccgcg gcctcgtcct cgacgagtgc
     3001 acccgccgcg gcatcgtcat cggttggcgg gaggcgccca cagcgttggc cgactccccg
     3061 gccagaccag ccaaggcgga tttgtcgtcc gaggcagctg cgcccctggc tatgccgcct
     3121 accgaggccg acggctagca tcggccaatg accgacacgt cactgccggg ttccttgagc
     3181 gagcctgagc ttgaaaacct tctggcggag aaccgtcgtt tcccgccggc tgcggacttc
     3241 atggcccagg ccaacgcaac ggccgacctg tacgaggaag ccgagcgcga ccccgaagcc
     3301 ttctgggcga ggcttgcccg cgagcggctc gcctgggaca cccccttcga gaccactctc
     3361 gagtgggagc caccgttcgc gaagtggttc ctcggcggca agatcaacgc ctcgtacaac
     3421 tgcgtcgatc ggcacgtcga gaacgggctc ggcgacaagg tcgcgtacca ctggatcggc
     3481 gagccgggcg acacgcggac tatcacctac gccgatctcc agcgcgaggt gtcgaaagcc
     3541 gccaacgcgc tgaaggagct cggcgtgaag tccggcgacg ttgtcgcgat ctacatgccg
     3601 atgatccccg agctgcccat cgcgatgctc gcctgcgcaa ga
//
LOCUS       DATLCO010000179         2623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3966794, whole genome shotgun sequence.
ACCESSION   DATLCO010000179 DATLCO010000000
VERSION     DATLCO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3966794"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /locus_tag="VMZ33_04485"
     CDS             complement(<1..1939)
                     /locus_tag="VMZ33_04485"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HUR16517.1"
                     /translation="MRVLFGAAVALCAVITLPPAQAQGALVSGFVESSSEQISAGVVY
                     QSGSVLAGLTERQVDVVTIDADAGASIRLNQAGDYAFGGGDVIEQALADSRDGNRILA
                     TVNGSLFNAVVREGEYVAATGLGFNVSDGELVNAGNPRARPEGVPGFGLDAFGAPFIG
                     VPHPTLTLTLPGDQIADVNRVNEFRFADQVALFTPRFGATTETDTPGDEFVIEGFSLP
                     FRQAGTHTGTITEVRRGMSSTPIGAGQVVLSVGSTAPSAASYATLEQGNQISFTMAVE
                     EPWATATQAVGGKDMLVVNGQNIVVDPVENRARSAIGINAGGDVMVLTVDTGTTARGL
                     NLIELADLMIERGAVQAMNLDGGTSSQMAVRRPGDVDASFVNALAEPTRRRVANALQV
                     VSTAPDGPLASLLVNPVTSRATIGSTVAFVAKGQDADLNGITVDPALVDWNVATMPGT
                     NPGDAPVLTHTETGITLAPTAAGEFDVNARLAELTATADLSVSPDVTPPTLAVTGLSL
                     EDASSVALIGAGLTLSWSAADDGQVARIQVQRKVGTSSWRNLAVADPSATSLTSRVAL
                     TKPVRFRARAFDVAGHASPWAVTGRVRVVRVDDTDSRVKLTDEWTRTTDPSALGGAFL
                     RSTTAGATATLTLKALQMQAVG"
     gene            complement(1996..>2623)
                     /gene="deoC"
                     /locus_tag="VMZ33_04490"
     CDS             complement(1996..>2623)
                     /gene="deoC"
                     /locus_tag="VMZ33_04490"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HUR16518.1"
                     /translation="AKKALAGSTVHVASVSTGFPSGQTYLDVKLLETRQAVEAGADEI
                     DMVIDRGAFLQGDYRHVFDEIVAVKEACGEAHLKVILETGELETYDNVRRASILAMAA
                     GGDFIKTSTGKVVPAATLPVTLVMMEAVRDFYRRTGKIVGIKPAGGIRTAKEAIQYLV
                     VLYETLGPRWMTPDLFRFGASSLLNDVLMQIRKERSGAYQGPNYFTVD"
BASE COUNT          406 a          880 c          878 g          459 t
ORIGIN      
        1 tgcccacagc ctgcatctgc aacgccttga gtgtcagcgt cgccgtggcg ccggcggtcg
       61 tgctccgcag gaacgccccg cccagggccg acgggtcagt cgtgcgcgtc cactcgtcgg
      121 tcagcttgac gcggctgtcc gtgtcgtcga cacggaccac cctgacccgg cccgtgacgg
      181 cccacggact cgcgtgacca gccacgtcga aggcacgggc tcggaacctg acgggcttgg
      241 tgagtgcgac gcgcgaggtc agcgacgtcg ctgacggatc agccacagca aggttgcgcc
      301 aggagcttgt gccgactttg cgttggacct ggatgcgcgc cacctgtccg tcgtcggccg
      361 cgctccagct caacgtcaac cctgcgccga taagcgccac gctgctcgca tcctcgaggc
      421 tgagtccggt caccgcgagc gttggcgggg tcacatctgg cgacactgag aggtcggctg
      481 tcgccgtcag ctcggccagc ctcgcattca cgtcgaactc gccggcggcc gtgggtgcca
      541 gggtaatgcc cgtctccgtg tgggtcagga ccggtgcgtc gcccgggttg gtgccgggca
      601 tcgtggccac gttccagtcg accagggccg gatcgacagt gatgccattc aggtccgcat
      661 cctggccttt ggccacgaac gcaacggtgc tgccgattgt ggcgcggctg gttacggggt
      721 ttacgagcaa gctggccagg gggccgtcgg gcgcggtgct cacgacctga agagcgttgg
      781 ccacccgtcg gcgtgtcggt tcggcgagcg cgttgacgaa gctggcgtca acgtccccgg
      841 gccgccgcac ggccatctgg ctcgaggtgc cgccgtccag gttcatggcc tggaccgcac
      901 cgcgctcgat catcaggtcg gccagctcga tgagattgag gccacgcgcg gtcgtcccgg
      961 tatcgacggt cagcaccatg acgtcgccgc cggcgttgat gcctatggcg gaccgggctc
     1021 ggttctccac cgggtcgacg acgatgttct ggccgttgac gacgagcatg tccttgccgc
     1081 cgaccgcctg cgtggctgtc gcccagggct cctcgacggc catcgtgaac gagatctggt
     1141 tgccctgctc cagagtcgcg taggacgcag cggagggcgc ggtgctaccg acagacagca
     1201 cgacctgacc ggcgccgata ggcgtgctgc tcatgccccg tcgaacctct gtaatcgtgc
     1261 ccgtatgggt gcccgcctgt ctgaatggca ggctgaagcc ctcgatgacg aactcatcgc
     1321 ccggcgtgtc ggtctctgtg gtcgcgccga agcgcggcgt gaacagggcg acctggtccg
     1381 cgaaccgaaa ctcgttgacg cggttgacgt ccgcgatctg gtcgcctggg agtgtcagcg
     1441 tcagcgtcgg atgcggcacg ccgatgaatg gtgctccgaa cgcatctagc ccgaagccgg
     1501 gaacgccctc cggtcgcgcc cgcgggttcc cggcgttgac aagctcgccg tcgctgacgt
     1561 tgaagccgag acccgtcgcg gcgacgtact cgccttccct cacgactgcg ttgaagaggc
     1621 tgccgttgac ggtggccagg atgcggtttc catcgcgcga gtcggcgagc gcttgctcaa
     1681 tgacgtctcc gccaccgaac gcatagtccc cggcctggtt caggcggatc gacgcgccag
     1741 catcggcgtc gatcgtgacg acgtcgactt ggcgctcagt cagcccggct agcaccgacc
     1801 cggactgata gacgactcca gccgagatct gctcactcga agactctacg aagccgctaa
     1861 cgagcgcgcc ctgggcctgg gcgggtggca gcgtaatcac cgcgcacaac gccacggcgg
     1921 cgccgaaaag aaccctcaat gcgattttcc cctcgacatt tcgcggctgg gggcatggtc
     1981 gcacgcccgc gcgtcttagt cgacggtgaa gtagttcggc ccctggtaag cgcccgagcg
     2041 ctccttgcgg atctgcatca ggacgtcgtt gagtagcgag ctggcgccga atcggaacag
     2101 gtcgggcgtc atccagcgcg gaccgagcgt ctcgtagagc acgaccaggt actggatcgc
     2161 ctccttggcc gtgcggatgc cgccggcagg cttgatgccg acgatttttc cggtgcgtcg
     2221 atagaagtcg cggacggcct ccatcatcac gagggtgacc ggcagcgtag ccgccggaac
     2281 gaccttgccg gttgacgtct tgatgaagtc acctccggcg gccatcgcca ggatgctcgc
     2341 gcggcgcacg ttgtcgtacg tctcgagctc gccggtctcc aggatcacct tgaggtgcgc
     2401 ctcgccacac gcctccttga cggccacgat ctcatcgaag acgtgccgat agtcgccctg
     2461 caggaatgcg ccgcggtcga tgaccatgtc gatctcgtca gcaccggcct cgacggcctg
     2521 acgggtttcg aggagcttca cgtcgaggta cgtctggccg gacggaaagc cggtcgacac
     2581 gctcgcgacg tggaccgtgc tgccggcaag cgccttcttc gcc
//
LOCUS       DATLCO010000180         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3973967, whole genome shotgun sequence.
ACCESSION   DATLCO010000180 DATLCO010000000
VERSION     DATLCO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3973967"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VMZ33_04495"
     CDS             complement(<1..1149)
                     /locus_tag="VMZ33_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16519.1"
                     /translation="MSLGPKLADPARLVTRGFVALTVATLAFFVAAGIVLPVAPTFAK
                     DALGADESGVGVAIASFSIASLILRPLVGWSSDRFGRRPLLIGGPLLTVAGLGLHLVA
                     TDLATFIAARALLGAGEGFFFVAAIAAGADLAPENRRGEAISFLSLSLYLGIAIGPPI
                     GEAMLAIAAGSYDTVWLAAIAVAVSAVALSLLVPESAPAVLARASATIKTANVRGPLI
                     HPAGLFPGFISLLGLWGMAGFFTFLPLHARELGMGGAGVPFVMYALIVVLLRLIGAKW
                     PDRYGGARVSGAALVVSALGLAVIGLLPTPEGLMAGTFIFAVGVAFIMPALLTLAVAR
                     VPPTERGTVSGTVTLFLDVSFGVAPVVLGLVANRAGYAPTFIVSAVLAA"
     gene            1259..>2215
                     /locus_tag="VMZ33_04500"
     CDS             1259..>2215
                     /locus_tag="VMZ33_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUR16520.1"
                     /translation="MNPSRETLDSFAKTLQGTLAYPGDEAYLQATQLWNGMIRKQPAA
                     VVQARSVEDVVGTINLARENRVELSIKGGGHNIAGLALCDGGITLDLSGMRDVQVDPE
                     AKIARVAPGCTLGDVDQATQPHALATTLGFVSATGTAGLTLGGGFGYLTRRFGWTVDD
                     LESVEIVTADGQLRQASRSTNEDLFWALRGGGGNFGVVTEFTFRLHEVGPKVTAGIIA
                     WPAAQADGVLRVFREVAESSPRELTSVVLRRNAPPAPWLPAEAHGTPIIAMVVCHSGD
                     LEQAQADLAPIKGHGQPLADLIQVKDYVAQQTMLDATQPKGMH"
BASE COUNT          412 a          761 c          756 g          286 t
ORIGIN      
        1 cgccgccagt acggctgaga cgatgaacgt gggcgcgtag ccagcccggt tggcgacaag
       61 cccgagcacg accggcgcaa ccccgaacga gacgtcgagg aagagggtga ccgtgccgga
      121 tacggtgccg cgctccgtcg gcgggactcg ggcgaccgcc agggtcagca gcgccggcat
      181 gatgaatgcg actcctacgg cgaagatgaa cgtgcccgcc atgagcccct cgggcgtggg
      241 gagcagcccg atcacggcca gccccaacgc cgacaccacg agcgcggcgc cggacacgcg
      301 ggccccgccg tagcggtctg gccacttggc gccgatgagg cgcaacagca ccacgatcag
      361 cgcgtacatc acgaacggca cgcccgctcc acccattccc agctcgcgcg catgcagcgg
      421 taggaaggtg aagaagccag ccatgcccca caaaccgagc aggctgatga accccgggaa
      481 caggcctgcg gggtggatca gcggcccgcg cacattggct gtcttgatgg tcgctgatgc
      541 ccgcgcgagc accgccggcg ccgactccgg cacgagcagc gacagtgcca cggcactcac
      601 cgcgaccgcg atcgcggcca gccaaaccgt gtcgtacgag ccagcggcaa tggccagcat
      661 ggcctcgccg atggggggcc cgatggcgat gcccaggtac agcgagagcg acaggaagct
      721 gatcgcctca ccgcgacggt tctcaggcgc caggtccgca ccagcggcga tcgcggcgac
      781 aaagaagaag ccctcgccgg cgccaagcag cgcgcgcgcc gcgatgaagg ttgccaggtc
      841 agtggctacg aggtggaggc cgagcccggc gacggtcagc aggggcccgc cgatgagcag
      901 cggccggcgc ccgaaccgat ctgacgacca gcccacgaga gggcgcagga tcaacgacgc
      961 gatgctgaac gacgcgatcg cgaccccgac gccgctctcg tcggcgccca aagcgtcctt
     1021 ggcgaaggtg ggcgcgacag gcagcacgat gcccgccgcc acgaagaagg cgagcgtcgc
     1081 taccgtcagg gcgacgaagc cgcgcgtcac caggcgcgcg ggatcagcga gcttcgggcc
     1141 caggctcatc gccgccgatt atcgtccggg ggtcggtcga actccagctc ccgataccct
     1201 tcgagcgacg tatgctcgcc gaagccgtcc cctgggcgac accttacgga ggaatgccgt
     1261 gaacccttca cgagagacac tcgattcgtt cgccaagacg ctgcagggga cgcttgcgta
     1321 tccgggcgac gaggcatacc tccaggcaac ccagctctgg aacggcatga tcaggaagca
     1381 gccggcggcc gtcgtgcagg ctcggtccgt cgaagacgtc gtgggcacga tcaacctcgc
     1441 gcgcgagaac cgtgtggagc tctcgatcaa gggcggtgga cacaacatcg ccggcctcgc
     1501 attgtgtgat ggaggcatca cgctcgacct ctccggcatg cgtgacgtcc aggtagatcc
     1561 tgaggcgaag atcgctcgtg tggcgccggg gtgcacgctc ggcgacgtcg accaggcaac
     1621 gcaaccgcac gccctggcca cgacgctggg tttcgtttcg gcgacgggaa cggccggtct
     1681 gacgctgggt ggtggcttcg gctacctgac gcgccgtttc ggctggacgg ttgacgatct
     1741 cgagtcagtc gaaatcgtga ctgctgacgg ccagctccgg caggcctctc ggtcaacgaa
     1801 cgaagacctg ttctgggcgc tgcgcggcgg cggaggcaac ttcggcgtcg tcaccgagtt
     1861 caccttccgg ctgcacgagg tcggcccgaa agtcaccgca gggatcatcg cgtggccggc
     1921 cgcccaggcg gacggcgtcc tcagagtctt tagggaggtg gccgaatcgt ccccgcgcga
     1981 gctcacgtcc gtcgtccttc gccgtaacgc tccgccggcg ccgtggctgc ccgcggaagc
     2041 tcacggcacg ccgatcatcg cgatggtggt gtgccactcg ggcgatctcg agcaggccca
     2101 ggctgacctg gcaccgatca agggccacgg ccagccgcta gcggacctca tccaggtcaa
     2161 ggactacgtc gcccagcaga cgatgctcga cgccacgcaa ccgaagggca tgcac
//
LOCUS       DATLCO010000181         2829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1744499, whole genome shotgun sequence.
ACCESSION   DATLCO010000181 DATLCO010000000
VERSION     DATLCO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744499"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1256
                     /gene="ftsA"
                     /locus_tag="VMZ33_04505"
     CDS             21..1256
                     /gene="ftsA"
                     /locus_tag="VMZ33_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HUR16521.1"
                     /translation="MDREAVLVALDVGTSKVVALIGEVTREEGVTVIGKGQMDSSGLK
                     KGVVVNIEQTVGSIRGAREAAERLSGYRIESAIVAVGGNHLESQNSRGAVAVSGARRE
                     VSREDVDRATEVARAVSIPSNREVLHVLPRGFVVDGQEGVKDPLGMSAIRLEVETHIV
                     HASATAVQNLTKCVQRADIRIDELVCASLAAGDAVLTDTERELGVAVADFGAGTIDLA
                     LYMDGSPFHTAVLPLGGNNVTNDIAIGLKTSLIAAEDLKIRFGTADLGAVEKDEEVEV
                     AGIGEGDATTASRYELAEIIEARMREVFEKIGAEIERAGFSGMLPAGVVMTGGASQLT
                     GAARLGREVLQAPVRVAGPTGVAGLTDHLLTPAFSTSLGLLMWGGRGVQGYASAQYET
                     EASAGMMGRMRDWARGLFP"
     gene            1265..1669
                     /locus_tag="VMZ33_04510"
     CDS             1265..1669
                     /locus_tag="VMZ33_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HUR16522.1"
                     /translation="MKHRELTVRTGSVRGLFDITGECASFVSGEGDGLLNVFVPHATA
                     GVVIMELGAGSDIDLVHQLDELLPRDDRWRHAHGSRGHGADHVLPLLVSASVSVAVIG
                     GELQLGTWQSVAILDPNSDNATRRVALTFLKG"
     gene            1888..>2829
                     /gene="ftsZ"
                     /locus_tag="VMZ33_04515"
     CDS             1888..>2829
                     /gene="ftsZ"
                     /locus_tag="VMZ33_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HUR16523.1"
                     /translation="MPLRSDSENFALIRVIGVGGGGSNAVNRMIRAELMGVEFIAVNT
                     DAQALLQSDAPHKIRIGDKITRGLGAGGDPGIGQRAAEEDAEKIYEALKDSDMVFITA
                     GMGGGTGSGAAPVIAEIARDLGALTIGVVTKPFSFEGARRRLNAEKSSEALKDKVDTL
                     ITIPNDRLKDVVNKETSILDAFRVVDDVLRQGVQGISDLITVPGLINLDFADVRTIMK
                     DAGSALMGIGRASGENRATEAAREAIASPLLEVNISGAQGVLFNVTGGPNLGLFEVDE
                     AAEIIRSTADPEANIIFGTVVDERMGDEISITVIATGF"
BASE COUNT          487 a          880 c          981 g          481 t
ORIGIN      
        1 ctttaggcga caatcggccc gtggatagag aggccgtact cgtcgcgctc gacgttggca
       61 ccagcaaggt cgttgcgctc atcggcgagg tgacgcgcga ggaaggcgtc actgtcatcg
      121 gcaagggcca gatggattcg tccggtctga agaagggtgt cgtcgtcaac atcgagcaga
      181 cggtcggatc catccgcggt gcgcgcgagg cagcggagcg gttgtcggga tatcggatcg
      241 aatcggccat cgtggctgtc ggcggcaacc acctcgagtc gcagaactcg cgtggagcgg
      301 ttgcggtcag cggtgcgcgg cgcgaggtct cgcgcgagga cgtggatcgc gccaccgaag
      361 tcgcgcgagc cgtcagcatc ccgtccaatc gtgaggtgct gcatgtcctg ccgcgtggct
      421 tcgtggtcga tggccaggag ggcgtcaagg acccgctcgg aatgagcgcg atccggctcg
      481 aggtcgagac gcacatcgtc catgcctcgg cgactgcggt gcagaacctg accaaatgcg
      541 tgcagcgcgc cgacatccgg atcgacgagc tggtctgcgc ctcgctcgca gcgggtgacg
      601 ccgtgctgac cgacaccgag cgcgagctgg gcgtcgccgt tgccgacttc ggcgccggga
      661 ccatcgatct ggcgctgtac atggacggct cgccgttcca cacggcggtg ctgccgctgg
      721 gtggcaacaa cgtgacgaac gacatcgcga tagggctgaa gacgagcttg atcgctgccg
      781 aggacctcaa gatccgtttc ggcacagccg atctgggcgc agtcgagaag gacgaagagg
      841 tcgaggttgc ggggattggc gagggggacg ccaccaccgc ctcccgctac gagctggccg
      901 agatcatcga ggcgcgcatg cgcgaggtct tcgagaaaat tggagcggag atcgaacgcg
      961 caggcttttc cgggatgctg cccgcgggtg tcgtcatgac cggcggtgcg tcgcagctca
     1021 cgggcgcggc tcggctcgga cgcgaagtgc tccaggcacc ggtacgggtt gccggcccga
     1081 cgggcgtggc cggtttgacc gatcacctgc tgacccccgc gttttccacg tccctcggcc
     1141 ttctgatgtg gggtggccga ggcgtccagg gctacgcaag tgcccagtac gagactgagg
     1201 cgtcggccgg gatgatgggc cggatgcgcg attgggcgag gggcctgttc ccgtagggct
     1261 ccgcatgaag caccgtgaac tcaccgttcg taccgggtcg gtgcgagggc tcttcgacat
     1321 cacgggcgag tgcgcctcgt tcgtgtctgg tgagggggac ggcctgctca acgtgttcgt
     1381 gccccacgcc acggccggcg tcgtgatcat ggagctcggc gcgggatcgg atatcgacct
     1441 ggtccaccag ctcgacgagc tgctgccccg tgacgatcgc tggcgccatg cccacggctc
     1501 ccgcggtcac ggcgccgacc atgtgctacc gctgctggtt tcagcctccg tcagcgtggc
     1561 ggtcatcggt ggtgagcttc agctcgggac gtggcagtcg gtcgcgatcc tggatcccaa
     1621 cagtgacaac gccacgcgac gcgtcgccct gaccttcctg aagggctaaa gccagcgcgc
     1681 agcgagtcac tgttgacggt tcgccgaggc tttccacagg tcgtccacac ccggcccgaa
     1741 atcggtaccg aaacaatgcc tacctcccga attcgcgtcg gccggtcaac ggcggcggga
     1801 ctatactcgg ccggtccgag cagatcctgg cgtcccgtgc ccgcgccatc ggtggctcgc
     1861 taaaaacgac aatttggagg gtgggtaatg ccgctgcggt ctgactcgga gaacttcgcg
     1921 ctcatccggg tcatcggtgt tgggggcggc ggaagcaacg ccgtcaatcg gatgattcgc
     1981 gccgagttga tgggcgttga gttcatagcg gtcaacaccg acgcacaggc acttctccag
     2041 tcggatgcgc cgcacaagat ccgcatcggc gacaagatca cgcgcggtct cggcgcgggc
     2101 ggcgaccccg gcatcggaca gcgagcggca gaggaagacg ccgagaagat ctatgaggcg
     2161 ctcaaggatt ccgacatggt gttcatcacc gccggaatgg gcggcggcac gggctccggc
     2221 gctgctcccg tcatagccga gattgcccgg gacctggggg cgctgaccat cggtgtcgtg
     2281 acgaagccct tctcgtttga aggtgctcgg cgccggctga acgccgagaa gtcgagtgag
     2341 gccctcaagg acaaggtcga cacgctcatc accattccca acgatcgttt gaaggatgtc
     2401 gtcaacaagg agacgtcaat ccttgacgcg ttccgagtcg tggacgacgt gctgcggcag
     2461 ggcgtgcagg gaatcagcga cctgatcacg gtgcccggcc tgatcaatct cgacttcgcc
     2521 gatgtccgca cgatcatgaa ggacgccggt tcggcgctga tgggcatcgg gcgtgcctcc
     2581 ggtgagaatc gcgctacgga agccgcccgc gaggcgattg ccagtccgct cctcgaagtc
     2641 aacatctcgg gcgcgcaggg ggtcctgttc aacgtcaccg gcggccccaa cctcggcctg
     2701 ttcgaggtcg acgaggcggc ggagatcatc cgctccacgg ccgacccgga ggccaacatc
     2761 atcttcggca cggtcgtgga cgagcgcatg ggcgacgaga tctcgatcac cgtcatcgcc
     2821 accgggttt
//
LOCUS       DATLCO010000182         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1000494, whole genome shotgun sequence.
ACCESSION   DATLCO010000182 DATLCO010000000
VERSION     DATLCO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000494"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..939)
                     /locus_tag="VMZ33_04520"
     CDS             complement(22..939)
                     /locus_tag="VMZ33_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HUR16524.1"
                     /translation="MAEGDDGDLIIDGDLVPRPLTPAAAPASEVWAVDGGQALVRDAR
                     CLQVYVVRAGRCCFVDRVATVEDEGDLRAHLVGTGQRGIELARVDGGLHLPANTAVDI
                     NLLRERVEWDGIERTLSEASPGALVLVDGDLRPDPRLPLGWVTELLADATARGITVVG
                     VTKHTSLSRGGAPLMGQLEREADAALGPRSRWWAAIGHSAAERGVPFTVVAARLDPDA
                     AYSFRLDIAEGIDPEVAIAQVACVSDDAAFPGYPYPLTVADRLAACPPWLREEAWMTL
                     EEHLRMAGVPGDVLHRAFDDRHRSMERAS"
     gene            1162..1965
                     /locus_tag="VMZ33_04525"
     CDS             1162..1965
                     /locus_tag="VMZ33_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HUR16525.1"
                     /translation="MPPRSLWSGAISFGLVNVPVKMVTAVSQKDVRFNQLHAKDHSRI
                     NQKRVCALDGEEVPFEEIVKGYEVRPGTYVIIDPDELDGLDPVATHTIDILEFVDLDQ
                     IDPVYFDKPYYLVPDRRSEKPYALLAQSLERSGKVGIAKFVMRTKEYLAAIRSTQGAL
                     VISTLLYGDEVVPIETLETPGLETELSERELKMAEALIESLTEKFDPDRYEDSYREKV
                     MDLIAKKDQGQEIVAQPEAEAPARVEDLMAALEASVQAARDKRGKSKKD"
     gene            1943..>2133
                     /locus_tag="VMZ33_04530"
     CDS             1943..>2133
                     /locus_tag="VMZ33_04530"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16526.1"
                     /translation="MASPKKIEVDVAGRRLTLSNLDKVLYPGAHFTKSEVIEYYARIA
                     PVMLPHLEGRPITFVRYPDG"
BASE COUNT          393 a          715 c          703 g          322 t
ORIGIN      
        1 ggccacgtgt gccgacggtg gtcatgacgc gcgctccatc gaacggtggc gatcgtcaaa
       61 ggcgcggtgt aacacgtcgc cgggaacgcc ggccatgcgc agatgctctt cgagcgtcat
      121 ccacgcttct tcgcgcagcc agggcggaca ggccgcgagc cggtcggcca ctgtgagcgg
      181 ataggggtag ccggggaacg ccgcgtcgtc ggagacgcag gcgacctgcg cgatcgcgac
      241 ctcagggtcg atgccttcgg cgatgtccag gcggaacgaa taggcggcgt ctggatcgag
      301 ccgcgcggcg acgacggtga acggcacgcc ccgttccgcc gcgctgtggc cgatcgcggc
      361 ccaccaccgg ctgcgcggcc cgagtgctgc gtcggcttcg cgctcgagtt gccccatcag
      421 cggagcgcca ccacgcgaca acgacgtgtg cttggtgacc ccgaccacgg tgatgccgcg
      481 tgcggtggcg tcggccaaca gttcggtgac ccagccaagg gggaggcgcg ggtcggggcg
      541 caagtcgccg tcgaccaaca ccagcgcgcc gggagacgcc tcgctcaacg tgcgctcgat
      601 gccgtcccac tcgacgcgtt cgcgcaacag gttgatgtcg accgctgtgt tcgccggcag
      661 gtgcaggcca ccgtcgaccc gggccaactc gatgccgcgc tggcccgtgc ccaccaggtg
      721 cgcccgtaga tcgccctcgt cctcgaccgt ggcaaccctg tccacgaaac agcagcgccc
      781 ggcccgcacc acgtagacct gcaggcagcg ggcgtcgcgg accagcgcct ggccgccgtc
      841 aaccgcccag acctcggagg caggcgccgc agcgggcgtg agggggcgag ggacgaggtc
      901 cccgtcgatg atcaggtcgc cgtcgtcacc ctcagccacg acaccggacg gcgcgccgag
      961 gatcgcagcg aggcgatcag cgacgtcatc aagcggtgcg gctcccagag gcatagaccg
     1021 atgttcccac aggggtgtga cacgctctcg gaccaccttg cgggcttagt acgggttttg
     1081 tgtgatgttt ggttcgctat agcgaaccaa acatcacaag aaccggacgg gcccagtcac
     1141 tcattcggcc acaatagggt catgcctccc cgttcattgt ggagcggcgc catcagtttt
     1201 ggcctcgtca acgtccccgt gaagatggtg acggccgtct cgcagaagga cgtgcgcttc
     1261 aaccaactcc acgccaaaga ccactcccgc atcaatcaga aacgcgtctg cgcgcttgac
     1321 ggcgaggagg tgcccttcga ggaaatcgtc aagggttacg aggtccggcc gggcacgtac
     1381 gtgatcatcg accccgacga gttggacggg ctcgaccctg tcgccactca cacgatcgac
     1441 atcctcgagt tcgtcgacct cgaccagatc gaccccgtct atttcgacaa gccgtactac
     1501 ctcgtgccgg atcgccggtc cgagaagccc tacgcgctgc tcgcgcagtc cttggagcgc
     1561 agcggcaagg tcggcatcgc caagttcgtg atgcgcacaa aggagtacct cgccgcgatc
     1621 cggtccaccc agggcgcgct cgtcatctcg acgctgcttt acggcgacga ggtcgtgccg
     1681 atcgaaacgc tcgagacgcc cggcctggag accgagctca gcgagcgcga attgaagatg
     1741 gccgaggcgc tgatcgaaag cctcaccgag aagttcgacc ctgaccgcta cgaggattcg
     1801 tatcgggaga aggtcatgga tctgatcgcg aagaaggacc agggccagga gatcgtggca
     1861 cagcccgaag cggaggcgcc ggcccgcgtc gaggacctca tggccgcgct cgaagccagc
     1921 gtgcaggccg cccgagacaa gcgtggcaag tccaaaaaag attgaggtcg acgtcgccgg
     1981 ccggcgattg acgctgtcca acctcgacaa agtgctgtat cccggtgcgc acttcacgaa
     2041 gtcggaggtc atcgagtact acgcgcgcat cgcgccggtg atgctgccgc acctcgaggg
     2101 acggccgatc acgttcgtgc gctatcccga tgg
//
LOCUS       DATLCO010000183         3782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2496000, whole genome shotgun sequence.
ACCESSION   DATLCO010000183 DATLCO010000000
VERSION     DATLCO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496000"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VMZ33_04535"
     CDS             <1..897
                     /locus_tag="VMZ33_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HUR16527.1"
                     /translation="EAERKFKEVSEANEVLSDPDKRKLYDQLGSNWQAYQHSGDDGPG
                     SAPGANPFAGFGGSSPGGGVRYEFRGNSEDLEGFSDFFRTFFGGGVGSQTGGGATQTR
                     VRTGASIEDLLAGLGADGPGKASNGRRQVAPQRLEAQAEMTLEEVDAGTKRLLEVNGR
                     RLEVNIPAGVRDGQKIRFSGVADGADAYITVRVKPHAAFTRAGSDLRSELPISLREAL
                     LGGEVPVRTLHGRVLLRIPPETQNGRSFRLAGKGLPHFAKDGRGDLYVKVTVVLPTGL
                     SDEAQAIAQQFLSAADQEDPRA"
     gene            994..>3782
                     /gene="clpB"
                     /locus_tag="VMZ33_04540"
     CDS             994..>3782
                     /gene="clpB"
                     /locus_tag="VMZ33_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HUR16528.1"
                     /translation="MKLDRYTEKAQEAILAAQRLATDADSPVLDAEHVLAALLQDRAG
                     IPFATLQKLEADPARLNVELAAVLSRRAKIQGGSLSLDPRTQQLIAHAEDEAKRLQDE
                     YVSTEHLLLAAARAGGDAQRLLESNGASHEQLLHALQAVRGGQRVTSQHPETTYQALE
                     KYGRDLTAEARAGRLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLA
                     QRIVRGDVPEGLKGKRVVGLDLGSLIAGAKYRGEFEERLKAVLKEVKDSDGQVVLFID
                     ELHTVVGAGAAEGAMDASNLLKPMLARGELHTIGATTLDEYRKHIEKDAALERRFQPV
                     FVDQPTVEESISILRGLRERYENHHGVRITDSALVAAAVLSNRYISERFLPDKAIDLV
                     DEAASRLRMEMDSMPVELDELERRRVQLEIEREALRKEKDEASKARLGALEKELADIS
                     ERANALKSRWDQEKGQIAELRASKQEIERVQHEIEEAERAADYARAAELKYGRLNELQ
                     AQLGQQEAGLSQRDGGQRLLKEEVDSDDIARIVATWTGIPVTRLLEGEQAKLIRMEDE
                     LHKRVVGQDEAVAAVSDAVRRARAGLKDPRRPIGSFIFLGPTGVGKTELARALAGFLF
                     DDDNAMTRIDMSEYMEKYAVSRLIGAPPGYVGYEEGGQLTEAVRRRPYQVILLDEIEK
                     AHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNVGSQFISSYAGSRDEQAYEG
                     MKRQVTDTLRQVFRPEFLNRVEEIIVFHALGEDALSEIVDLLLDDLSRRLVEHDLRLD
                     VTPGARTLIAREGHDPAYGARPLRRAIQRLVENPLAKALLEGRFAAGTTIRVDADSTS
                     GTLVFSNGEQTVVATATDERRDIRGTRSETEPELEPANAGSDSKSLLNYPAADVRVEP
                     QRTQFSPPPADDAGVP"
BASE COUNT          723 a         1246 c         1254 g          559 t
ORIGIN      
        1 gaggctgagc gcaagttcaa ggaggtcagc gaggccaacg aggtcctctc cgaccccgac
       61 aagcggaagc tctacgacca gctcggttcg aactggcagg cctaccagca ctccggcgac
      121 gacggcccgg gtagcgcgcc cggagccaac ccgtttgcgg gcttcggagg ctccagtccc
      181 ggcggcggcg tccgctacga gttccgaggc aattcggagg atctcgaggg cttcagtgac
      241 ttcttccgaa ccttcttcgg cggcggcgtc ggcagccaaa ccggcggtgg cgccacgcag
      301 acacgtgttc ggacgggcgc atcgatcgag gacctgctgg cgggcctggg cgccgatggg
      361 ccaggcaagg ccagcaacgg tcgtcgacag gtcgctcccc agcgacttga ggcgcaagcg
      421 gagatgacgc tcgaggaggt cgatgcgggc acgaagcgac tgctcgaagt gaatggccgt
      481 cggctggagg tgaatatccc ggctggcgtc cgcgacggtc agaagattcg cttctcaggc
      541 gtggctgatg gcgcagatgc atacatcacc gtccgcgtga agccgcacgc cgccttcacc
      601 cgcgcaggtt ctgatctccg cagtgagctg cccatctccc tgcgcgaggc actgctgggc
      661 ggggaggtcc cggtgcgaac gctccacggc cgagtgctac tgcggatccc gcctgagaca
      721 cagaacggtc gcagcttccg gttggccggc aagggtttgc cgcactttgc caaagacggg
      781 cgcggcgatc tctacgtcaa ggtcactgtc gtgcttccaa ccggtttatc ggacgaagca
      841 caggcgatcg cgcaacagtt cctgagcgcg gccgatcaag aggatccacg cgcctagcca
      901 ccgcgcgccc gtcacccctg gatggttgac agtcgacacc ataccgctgt caactacctt
      961 gacactcatg ttgtcaactt ctatgctgta gaagtgaagc tcgaccgata cacagagaag
     1021 gcgcaggaag caatcctcgc cgcacaacgc ctcgccacgg acgcagattc gcccgttctc
     1081 gacgcggagc acgtcctggc cgcgctcctg caggaccgcg caggcattcc attcgcaaca
     1141 ctccagaagc ttgaggccga tccggcacgg ttgaacgtgg agctggccgc ggtcctgtcg
     1201 cggcgcgcga aaatccaggg cggctcgctc agcctcgatc cgcggacgca acagctcatc
     1261 gcgcatgccg aagacgaggc caagcgcctt caggacgaat acgtttccac cgaacacctg
     1321 cttctcgctg cggcacgcgc gggcggcgac gcccagcggc tcctggagtc gaacggcgct
     1381 tcgcacgaac agctcctcca cgcgctccag gccgtccgcg gtgggcagcg cgtcaccagt
     1441 cagcacccgg agaccacgta ccaggcgttg gaaaagtacg gccgcgacct gaccgccgaa
     1501 gcgcgagcgg gacgcctgga ccccgtcatt ggacgtgacg aggagatccg ccgaaccatc
     1561 caggtcctct cgcgccgcac gaagaacaat ccggtgctca tcggtgagcc gggcgtcggc
     1621 aaaacggcga tcgcagaggg actggcccag cgcatcgttc gtggcgacgt acccgaaggc
     1681 ctcaaaggaa agcgcgtcgt cgggctagac ctcggctcgc tcatcgccgg cgccaagtac
     1741 cgcggcgagt tcgaggagcg cctcaaggcg gttctgaagg aggtcaagga ctccgacggg
     1801 caggtcgtgc tgttcatcga cgagctgcac accgttgtcg gcgccggcgc cgccgagggc
     1861 gcgatggacg ccagcaacct gctgaagccg atgctcgctc gcggcgagct gcacacaatc
     1921 ggcgccacga cgctcgatga atatcgcaag cacatcgaga aggacgccgc gctggagcga
     1981 cgctttcagc ctgtcttcgt cgaccagccg acagtcgagg agagcatctc gatccttcgt
     2041 ggcctgcgcg agcgctacga gaaccatcac ggcgtgcgca tcacggactc ggcgcttgtc
     2101 gccgcggccg tcctgtcgaa ccgatacatc agcgagcgct tcctccccga caaggcgatc
     2161 gacctcgtgg acgaggccgc gagccgactg cgcatggaga tggactccat gccggtggag
     2221 ttggacgagc tcgaacgtcg acgcgtgcag ctggagatcg agcgcgaggc gctacgcaag
     2281 gagaaggacg aggcctccaa ggcccgcctg ggcgcgctgg agaaagagct ggccgacatc
     2341 agcgagcgcg ccaacgccct caagagtcga tgggatcagg agaagggtca gatcgccgag
     2401 ctccgagcca gcaagcagga gatcgagcgc gtccagcacg agatcgagga agccgaacgg
     2461 gcagccgact acgcgcgcgc ggccgagctc aagtacggcc gcctgaacga gctgcaggcc
     2521 cagctcggac agcaggaggc cggcctgtcg cagcgtgacg gcggccagcg gctgctcaag
     2581 gaagaggtcg actccgatga catcgcgcgg atcgtggcga cttggacagg cattccggtc
     2641 acgcgcctcc tggagggcga gcaagcgaag ctgatccgaa tggaagacga gctccacaag
     2701 cgcgtcgtcg gccaggacga ggcggtggct gcggtgtcag acgcggtccg tcgtgcgcgc
     2761 gcgggcctga aggatccccg ccgacccatc ggctctttca tcttcctggg cccgaccggt
     2821 gtcggaaaga cggagctggc gcgtgccctc gcggggttcc tgtttgacga cgacaacgcc
     2881 atgacccgca tcgacatgag cgagtacatg gagaagtacg cggtgtcgcg gctcatcggc
     2941 gcaccgccgg gctacgtcgg gtacgaggag ggcggccagc tgactgaggc tgtccggcgc
     3001 cggccgtacc aggtgatcct gctcgacgag atcgagaagg cgcaccccga cgtgttcaac
     3061 gtccttctcc aggtattgga cgacggccgg ctgaccgacg gacagggccg gacggttgac
     3121 ttcaagaaca ccgtgatcgt catgaccagc aacgtcggct cgcagttcat ctcgagctac
     3181 gccggctcgc gtgatgagca ggcctacgag gggatgaagc ggcaggtcac ggacacgctg
     3241 cgccaggtct tccgcccgga gttcctgaat cgcgtcgagg agatcatcgt tttccacgcc
     3301 ctcggcgagg acgcgctcag tgagatcgtg gatctgctcc tggacgatct gtcacgccgg
     3361 ctggtggagc acgacctccg acttgacgtt acgccgggtg cccggacact gatcgcccgc
     3421 gagggtcacg acccggccta cggcgctcgg ccgctccgcc gcgcaattca gcgcctcgtt
     3481 gagaacccac tcgcaaaggc actcctcgag ggccggttcg cggccgggac cactattcgc
     3541 gtggacgccg attcgacaag cggcacgctc gttttcagca atggcgagca aacggttgtg
     3601 gccactgcca ccgacgagcg gcgcgacatc cgcggaacgc gatctgaaac agagccagag
     3661 ctggagcccg cgaatgcggg aagcgactcc aagagccttc tgaactaccc tgcggccgac
     3721 gtgcgcgtcg agcctcagcg aacccaattc agtccgcccc ccgccgatga cgcgggcgtc
     3781 cc
//
LOCUS       DATLCO010000184         2394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6839363, whole genome shotgun sequence.
ACCESSION   DATLCO010000184 DATLCO010000000
VERSION     DATLCO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6839363"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VMZ33_04545"
     CDS             <1..1109
                     /locus_tag="VMZ33_04545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HUR16529.1"
                     /translation="LARQLNSQRELLRVTESILSTLDTRALLQEIAERLNALLQVDNM
                     CVDVHDARAGLLRPIFAHGIHHDQYLAATIRDDQGVGGYVLRTGEAQLVHDQLTDERV
                     AHFAGVGPEAGAMIVAPLRGAEGVRGVFTIERLGAHARFDEEEFELVKLFAAHVSIAL
                     QNAEAHRAVELRAETDALTGLWNHGAMIDQIDQLAKGGGRFAMLMVDLDFFKAYNDSL
                     GHQAGNVMLQEISQLLRASCRESDNVYRFGGDEFALILSNTSLAGARLVAQKVQAAVR
                     SVNEGRRLPTTFDCSIGIAVFPKDGTDRQAIILAADRACYAAKRAGRGRIATAAEGLA
                     LATEFEPTEPTQFIPSEAPVRRETSERRPSYSAA"
     gene            complement(1094..1999)
                     /locus_tag="VMZ33_04550"
     CDS             complement(1094..1999)
                     /locus_tag="VMZ33_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HUR16530.1"
                     /translation="MARRFMRPITTPAASRIPQDGRRPFVSLTSDWGVRDPSPAICRA
                     VALGIAPDALIVDITHEVQKYNIRHGALMLWCALPFMPVGAHVCVVDPGVGTARRPLA
                     IETARGDYLIGPDNGVLLPGAERLGGVVRVHRIENAQYRLPVVTSTFHGRDLFTPAAA
                     HLAVGVPLDSIGPAIDSASLTPLDSWPEAVIEDGELTATLLYIDTFGNIKLGALAGQL
                     AEALPGLQHGETVSLRVEHSKGREMQMPWAQTFGQVSKGDQLLYEDSYGRLCIAENQG
                     DAAQTLKLEEGVTLRITRASAQAAE"
     gene            2038..>2394
                     /locus_tag="VMZ33_04555"
     CDS             2038..>2394
                     /locus_tag="VMZ33_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HUR16531.1"
                     /translation="MSDMTATGRERGSVKWDRAARYLKIARVLHEHPDGISAQGLADL
                     IGVSKRTIYRDLDHMDSDAGIPIWIDSGKYGLESSVFLPPLALTLHEATTLFLAARML
                     AKTSDEHDTELIGAFVK"
BASE COUNT          413 a          772 c          802 g          407 t
ORIGIN      
        1 ccctggcccg ccagctcaac agccagcgcg agctcctgcg cgtgaccgag tcgatcctct
       61 ccacgctcga cacccgggct ctgctccagg aaattgcgga gcgtctcaac gcgctgctcc
      121 aggtcgacaa catgtgcgtc gatgtccacg acgcgcgggc gggccttctt cggccgatct
      181 ttgcccatgg catccatcac gaccagtacc tggcggcgac cattcgcgac gaccagggcg
      241 tcggcggcta tgtcctccga accggtgagg cgcagctcgt ccatgaccag ctgaccgacg
      301 aacgcgtcgc gcattttgcc ggagttgggc cggaggccgg agcgatgatc gtcgctccgt
      361 tgcgtggcgc ggaaggcgtg cgtggcgtgt tcaccattga acggctcggc gcccatgcgc
      421 gctttgatga ggaggagttt gagctcgtca agctctttgc tgcacacgtt tccatcgcgc
      481 tccagaacgc tgaggcgcac cgcgccgtcg agctacgcgc cgaaacggat gcgttaactg
      541 ggctgtggaa ccatggagcg atgatcgacc agatcgacca gctggcaaag ggaggcggtc
      601 gcttcgcgat gctcatggtc gatctcgact tcttcaaggc gtataacgac agccttggcc
      661 atcaggcggg caacgtaatg ctccaggaga tctcccagct cctgcgggcc tcgtgccgcg
      721 agagcgacaa cgtctaccgg ttcggcggcg acgagttcgc gctgatcctg tcgaatacct
      781 cgctggcggg agcccgactg gtcgcccaga aggtccaggc tgcggtccga tcggtcaacg
      841 agggccgtcg tctgccaacg accttcgact gctccatcgg catcgcggtt ttcccgaagg
      901 acggcaccga tcggcaggcc atcatcctcg ccgccgaccg cgcctgctac gcggccaagc
      961 gcgccggtcg tggccggatc gcgactgccg cagagggcct cgcccttgcg accgagttcg
     1021 agccaacgga gccgacgcag ttcatcccgt ccgaagcgcc ggtgcggcgt gaaacgtcgg
     1081 agagaaggcc gtcctactcc gcagcctgag ccgatgcgcg ggtaattcga agcgtcacgc
     1141 cttcttcgag cttgagcgtc tgcgcggcat caccctggtt ctcggcgatg cacagccgcc
     1201 cgtatgaatc ctcgtagagc agctggtccc ccttgctgac ctgcccgaac gtctgcgccc
     1261 agggcatctg catctctcga ccttttgagt gctcgacgcg caagctcacg gtctccccgt
     1321 gctgcaggcc gggtagtgcc tcggccagct gccccgccag ggcgccgagc ttgatgttgc
     1381 cgaacgtgtc gatgtagaga agtgttgccg tcagctcgcc gtcctcgatc acggcctcgg
     1441 gccaggagtc gagtggggtc aggctggcgg agtcgatcgc gggaccgatc gaatcgagcg
     1501 gcacgcccac ggcaaggtgc gcggccgcgg gcgtaaacag gtcgcggccg tgaaaagtgg
     1561 acgtgaccac cgggagccgg tattgggcgt tctcgatgcg atggactcgg accacgccgc
     1621 cgagccgctc agcgccgggg agcagcacac cgttgtccgg gccgatcagg tagtcgccgc
     1681 gtgccgtctc gatggcgagt ggtcggcggg ccgtgccgac acctggatcg acgacgcaga
     1741 cgtgtgcccc aaccggcatg aacggcagag cgcaccacag catcagcgca ccgtgccgaa
     1801 tgttgtattt ctgaacttcg tgcgtgatgt ccacgatgag cgcgtcgggc gcgatgccga
     1861 gtgccaccgc gcggcagatc gccggcgatg ggtcgcggac gccccagtcg gacgtcaggc
     1921 tgacgaacgg ccggcgccca tcctggggga ttcgggaggc tgctggcgtg gtgatcgggc
     1981 gcatgaatcg gcgtgccatc tcggcccgat ggtaggggcg ttaccgttca ggcacatatg
     2041 agcgatatga cggcaaccgg gcgagaacga ggaagcgtca agtgggaccg tgcggcgcgc
     2101 tatctcaaga ttgcgcgggt gctgcacgag cacccagacg gcatatcggc gcaggggctg
     2161 gccgacttga tcggggtatc gaagcgcacg atctaccgcg acctcgatca catggacagc
     2221 gacgccggaa tcccgatctg gatcgacagc gggaagtacg gactggaaag ctcagtcttc
     2281 ctgccgccgt tggcactgac tttgcacgag gccaccactc tctttctggc ggcgcggatg
     2341 ctggccaaga cgagcgacga gcacgacacg gagctgatcg gcgcgttcgt gaag
//
LOCUS       DATLCO010000185         5126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9201440, whole genome shotgun sequence.
ACCESSION   DATLCO010000185 DATLCO010000000
VERSION     DATLCO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9201440"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..2379)
                     /locus_tag="VMZ33_04560"
     CDS             complement(130..2379)
                     /locus_tag="VMZ33_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUR16532.1"
                     /translation="MSGSGKSSLVFETIAAESQRLINETYTAFIQSFMGTPSRPDVDS
                     LENLSAAIVVDQERMGANVRSTVGTVTDAYAMLRILFSRLGEPRIGPSTAFSFNVPAA
                     AIRGEIKLEKSDSDWEQREFATSGGMCPECEGLGKVSSVDIDRLVDRSKSLAEGPINF
                     PNFQLGGWYLRIFLESGFFDNTKPLRDFTTEEWDRFLNGAEAKTKVKTELNINLTYLG
                     LLERIKKSYLVKDRESLQAHIRAAVERISTQAPCPACGGTRLNELARSSLVAGKNIAD
                     VSAMQISDLASWLRELGAVSQGVRPIVDNLVSLLDDFATIGLGYLSLDRESSTLSGGE
                     SQRTKMVRHLGSPLSDMTYVFDEPTIGLHAHDVEQMNSLLLRLRDKGNTVLVVEHDPE
                     VMRVADHVVDMGPGAGTHGGEVVYEGPFAGLAAAGTLTGRHLGERQPLKESVRPSRGS
                     IAIRKASLHNLRDVSVDVPLGVMVAVTGVAGSGKSSLIHGCLPRLDPKVTVVDQSAIK
                     GSRRSNPATYTGMLDGIRKAFASANGVKPALFSANSEGACPDCRGLGLIYTDLVMMAG
                     VVTVCETCGGKRFTEEVLGYKLRARNIAEVLDMSVEEAQAFFTEKPIRVMLDRLADVG
                     LGYISLGQMLNTLSGGERQRLKLAIEMSGDADVFVLDEPTSGLHMHDVDNLIGLMDRL
                     VESGRTVVVIEHNLDVVARADWVIDLGPGAGHDGGAIVFEGPPAALAKSKTSLTGQHL
                     RRRTESMAT"
     gene            complement(2490..2948)
                     /locus_tag="VMZ33_04565"
     CDS             complement(2490..2948)
                     /locus_tag="VMZ33_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16533.1"
                     /translation="MPAKRTTKSKSGEVWTDEERAAMQESARERKKAAKAGSPGEREQ
                     GESDVQAKIAEMPQDDRKMAARIHELVTSAAPDLVPRTYYGMPAYAKDGKVVCFFQAK
                     SKFKVRYSTFGFQPDAKLDEGEIWPTSFAVVELTAPVEKRLTELVRKAAS"
     gene            complement(2970..3398)
                     /locus_tag="VMZ33_04570"
     CDS             complement(2970..3398)
                     /locus_tag="VMZ33_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR16534.1"
                     /translation="MMKLNVASIFVLDKDEALDFYVNKLGLEKGSDVRQGDYRWLTVR
                     VPGETGTEIQLEEPGAPLHDEQTAAQLRELISKGAMTGLVFVTDDVRGLYDTLKERGV
                     TDFTQEPTDHFYGTDMGLRDPFGNAIRVLQQGKVREPATA"
     gene            complement(3439..3891)
                     /locus_tag="VMZ33_04575"
     CDS             complement(3439..3891)
                     /locus_tag="VMZ33_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR16535.1"
                     /translation="MSREAEDLNRRLLRARDAMDRAYAEPLNVRAVAAVAHISEAHFS
                     RSFRAAFGETPHRYLQRRRVERAMFLLRETDRSVTEICFDVGFTSLGTFSRTFSEITG
                     ASPTTYRLKNSPIDAPHCFQMAMSRPRLAADVNPRSSSFGEAQSAEAA"
     gene            complement(3896..5023)
                     /locus_tag="VMZ33_04580"
     CDS             complement(3896..5023)
                     /locus_tag="VMZ33_04580"
                     /inference="COORDINATES: protein motif:HMM:NF042779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOPRIM nucleotidyl transferase/hydrolase
                     domain-containing protein"
                     /protein_id="HUR16536.1"
                     /translation="MTSRLASVSVRGLGGLPDVELGLKPMTALIGPRGAGKSRLLAAL
                     AWLLTGDPALDGGARSPVDSKLSVTAELETDDGGSGKRTITGRPGEARRADLPPTRFL
                     SARERISPLPEDAPLGWSDAAQAERMVERIAEERLAGMKGEILLIEEPELMLTPHQQR
                     HLYNLLRRFVEQGNQVIYSTRSPALLDATRYDEIVRLDRTTEGMRIRRAPDQLLTELQ
                     RVHLAAEFDHERNEMFFATSVVLVEGQTERQSFPTIFNRLGYDPDALGISIVECGGKG
                     NLILIARVLAELRIPHLIVHDSDRGRPGQSANDYIKRNAGRAPVFQLHPDFEAVAGIR
                     SHDHKVFNAVRRFAKMPEEKIPAIFHEIADTAADLASGERQ"
BASE COUNT          802 a         1678 c         1706 g          940 t
ORIGIN      
        1 cagtcctcaa cgccgtcgga gttgtggaag gctcgagcag ttgttcgtct ttcgctcatg
       61 gcccgatgct agggccgcca gcggcaggtt cgaatgacgt ccgggaccgc gccgaggcgg
      121 gtgcgttcat taggtggcca ttgactcggt tcggcggcgg agatgctggc cggtcagcga
      181 cgtcttggac ttagcgagcg cggcgggtgg gccttcgaac acgatcgccc cgccgtcgtg
      241 gccggcgcct ggcccgaggt caatgaccca gtccgcgcgc gcgacaacgt cgaggttgtg
      301 ctcgatgacg acgaccgttc ggccggactc gaccagccga tccatcaggc cgatcaggtt
      361 gtcgacgtcg tgcatgtgca ggccggacgt cggctcgtcg agcacgaaga cgtcggcgtc
      421 gccgctcatc tcgatggcca gcttcagccg ctgacgctcg ccgccggata acgtgttgag
      481 catctgcccg agcgagatgt agcccagccc tacgtcagcg agtcggtcga gcatcacgcg
      541 gatcggcttt tccgtgaaga acgcctgagc ctcttcgacg gacatgtcga gcacctcggc
      601 gatgttcctg gcgcgcagct tgtacccgag cacctcctcg gtgaaccgct tgccgccgca
      661 tgtctcgcag accgtcacga cgccggccat catcaccagg tcggtgtaga tgaggcccag
      721 gccgcggcag tcggggcagg caccctccga gttggcgctg aacagtgccg gcttgacccc
      781 gttggccgat gcgaaggcct tccgaattcc gtcaagcatg ccggtgtagg tcgccgggtt
      841 ggagcggcgc gagcccttga tggccgattg atcgaccacg gtcaccttgg gatccagtcg
      901 gggcaggcag ccgtggatga gcgagctctt gcccgatcca gccacgcccg tgacggccac
      961 catcaccccc agcggcacgt ctaccgacac gtctcgcagg ttgtgcagcg acgccttccg
     1021 aatggcgatc gacccgcgcg aaggccgcac tgactccttg agcggctgcc gctccccgag
     1081 gtggcggccg gtcaacgtcc cggccgccgc caggcccgcg aaaggcccct cgtagaccac
     1141 ctcgccgccg tgcgtgcctg cgccgggtcc catgtcgacc acgtggtcgg cgacgcgcat
     1201 cacctcggga tcgtgctcaa cgactagcac ggtgttgccc ttgtcccgca ggcggaggag
     1261 cagcgagttc atttgctcga cgtcgtgggc gtgcaggccg atcgtcggct catcgaacac
     1321 gtacgtcatg tccgacagcg gtgatcccag gtggcgaacc atcttcgtcc gctgcgactc
     1381 gccgccggac agcgtcgagg attcgcggtc gagagacagg tagccgagcc cgatcgtcgc
     1441 gaagtcgtcc agtagcgaga ccaggttgtc gacgataggt cgcacgccct gcgacaccgc
     1501 gcccagctcg cgcagccacg atgcgaggtc gctgatctgc atcgcgctga cgtcggcgat
     1561 gttcttgccg gccacgagcg acgaccgcgc gagctcgttg agacgcgtgc cgccacacgc
     1621 cgggcacggt gcctgcgtcg agatgcgctc aaccgccgcc cgaatgtgcg cctgcagcga
     1681 ctcgcggtcc ttcaccaggt acgacttctt gatccgctcg agcaggccga ggtaggtgag
     1741 gttgatgttc aactcggtct tgaccttcgt cttcgcctca gcgccgttga ggaatcggtc
     1801 ccactcctcg gtcgtgaagt cgcgcagcgg cttggtgttg tcgaagaagc ccgactccag
     1861 aaagatccgg agataccacc caccgagttg gaagttcggg aagttgatgg ggccttcggc
     1921 cagcgatttc gaccgatcga ccaaccggtc gatgtccacg ctggacactt tgcccaggcc
     1981 ctcgcactcg ggacacatgc cgcccgaggt cgcgaactca cgctgctccc agtcgctgtc
     2041 cgatttctcg agcttgatct cgccgcggat cgccgcggcg ggcacgttga aggagaaggc
     2101 ggtcgaaggc ccgatacggg gctcgcccag cctgctgaag aggatccgca gcattgcgta
     2161 ggcgtcagtg acggtgccga cggtcgagcg gacgttcgcg cccatccgtt cctggtcgac
     2221 gacgatcgcc gctgacaggt tctcgagcga atcgacgtcc ggccgcgacg gcgtgcccat
     2281 gaacgactgg atgaacgccg tgtacgtctc gttgatcagc cgctgtgact cggcggcgat
     2341 cgtctcgaag acgagcgagc ttttgcccga gcccgacacg ccggtgaata cggacagccg
     2401 gcgcttcggg atctcaaggc tgacgttctt gaggttgttc tcgcgcgccc cccgaacgcg
     2461 aatcgcgccc cagtccggcc ccgcgccgct cagctcgccg ccttccggac gagttcggtg
     2521 agccgcttct cgacgggtgc cgtcagctca accactgcga aagatgtggg ccaaatttcg
     2581 ccctcgtcga gctttgcgtc cggctgaaat ccgaaagtcg agtagcgcac cttgaatttc
     2641 gacttggcct ggaagaagca gacgactttg ccgtccttgg cgtacgccgg catgccgtag
     2701 tacgtcctgg gcacgaggtc cggcgccgcg ctcgtcacaa gctcgtgaat gcgcgcagcc
     2761 atcttgcggt cgtcctgggg catctcggcg atcttcgcct ggacgtcgct ctcgccctgt
     2821 tcgcgctccc caggcgaccc ggccttcgcg gctttcttgc gctcccgagc gctctcctgc
     2881 atggcggcgc gctcttcgtc ggtccagacc tcgccggact ttgactttgt tgtccgtttg
     2941 gcgggcatgc tcgttgctcc ttgtgtcggt caggcggttg ctggctcgcg gaccttgccc
     3001 tgttgcagga cgcggatcgc gttgccgaac gggtcgcgga ggcccatgtc ggtcccgtag
     3061 aagtggtcgg tcggctcctg cgtgaagtcg gtcacgccgc gctccttgag cgtgtcgtac
     3121 aggccgcgca cgtcgtcggt cacgaacacg aggccagtca tcgcgccctt ggagatgagc
     3181 tcacgcagct gggcagccgt ctgctcgtcg tggagcggtg cgcccggctc ctcaagctgg
     3241 atctctgtgc ccgtttcgcc ggggacgcga acggtcagcc agcggtagtc gccctgacgc
     3301 acgtcgctgc ccttctcgag tccgagcttg ttcacgtaga agtcgagcgc ctcgtccttg
     3361 tcgaggacga agatcgaagc gacgttcagc ttcatcattt cagttctccc ggccactgct
     3421 tgttagttcg acctgaggct aggcagcctc ggccgattgc gcttctccaa aactgctcga
     3481 tcgcggattc acgtcggcgg ctagacgtgg gcgtgacatc gccatctgga agcaatgggg
     3541 cgcgtcgatc ggcgaattct tgagacggta ggtcgtcggt gaggcgcccg tgatctcgct
     3601 gaacgtccgg ctgaatgtgc caaggctcgt gaagccgaca tcgaagcaga tttcggtgac
     3661 gctgcggtcg gtctcgcgca agaggaacat ggcgcgctcg actcgtcggc gctgaaggta
     3721 gcggtgcggg gtctcgccga aggcggcgcg gaagctgcgg ctgaagtgcg cttcggagat
     3781 gtgcgcgacg gccgcgacgg cacgaacgtt gagtggctcg gcgtaggcgc ggtccatggc
     3841 gtcgcgtgcg cggaggaggc gcctgttcag gtcctctgct tcacggctca cagggctact
     3901 ggcgctcccc ggaggcgagg tcggcggccg tgtcggcgat ctcgtggaag atcgccggga
     3961 tcttctcctc gggcatcttc gcgaagcggc gaacagcgtt gaacaccttg tggtcgtggc
     4021 tgcggatgcc agccacggct tcgaagtcgg gatgcagctg gaacaccggg gcgcggccgg
     4081 cgttgcgttt gatgtagtcg tttgcgctct ggccggggcg cccgcggtcg gagtcgtgga
     4141 cgatgaggtg cgggatgcgg agctcggcga gaacgcgcgc gatcaggatc aagttgccct
     4201 tcccgccgca ctcgacgatg gagatgccga gcgcgtccgg gtcgtagcca agcctgttga
     4261 agatggtcgg gaacgactgt cgctcggtct ggccctcgac gagaaccacc gaggtggcga
     4321 agaacatctc gttgcgctca tggtcgaact cggcggcgag atggacgcgc tgcagctcag
     4381 tcagcaactg atcaggcgcg cggcgaatgc gcatcccctc cgtcgtgcga tcgaggcgca
     4441 cgatctcgtc gtagcgcgtc gcgtccaata acgccggtga cctcgtggag tagatgacct
     4501 ggttgccctg ctcgacgaat cggcggagca ggttgtagag gtggcgctgc tgatgaggcg
     4561 tgagcatcag ctctggctct tcgatgagca ggatctcgcc cttcatgccc gccaggcgct
     4621 cctcagcgat gcgctcaacc atgcgctcgg cctgggcagc gtcgctccac ccgagcggcg
     4681 catcctcggg gagcggcgaa atgcgctcgc gcgcgctcag gaagcgcgtc ggcggcaggt
     4741 cagcccgacg cgcctcgccg gggcgaccag tgattgttcg cttgcccgag ccgccgtcgt
     4801 cggtctccag ctcagcggta acggacagtt tgctgtcaac cgggctccgc gcgcctccgt
     4861 ccaacgccgg gtccccggtg agcagccatg ccagcgcggc caggagccgc gacttcccgg
     4921 cgccgcgcgg cccgatgagc gccgtcatcg gcttcagccc cagttccacg tcgggcaagc
     4981 ccccaaggcc gcggaccgag acggaggcga ggcgcgaggt cacgcggggc accttatgcg
     5041 agatatcagc cgactacaca tttccgaccg ttgccgacct gcaacaccct ccgtacctgt
     5101 acttcagtcc ccaaagtacg gcttcc
//
LOCUS       DATLCO010000186         2953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1011156, whole genome shotgun sequence.
ACCESSION   DATLCO010000186 DATLCO010000000
VERSION     DATLCO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011156"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /gene="pgmB"
                     /locus_tag="VMZ33_04585"
     CDS             <1..1416
                     /gene="pgmB"
                     /locus_tag="VMZ33_04585"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HUR16537.1"
                     /translation="LLGDETQTQVTLDEFLTVADGIYIQEPREDDVVEQFDGYFGRRE
                     VDLSEYAERSESMQALLGMVGLAETQVIKQPDVLMLAALLPETFTDAQLVANYDYYNA
                     RTDHSHGSSLGPGIQALLAARLGRLDEAYEHFQRAARVDLADVRGNTADGTHAASAGA
                     LWQAIVFGFAGVNLEGEKVRTKPQLPPHWRRLKFRIAHRGRTVDIDLRPTPQSTPGAE
                     AIRGLIFDLDGVVTDTAESHYLAWQRLADEEGLPFDRAANEALRGISRRQSLALLLGT
                     RSVSDEQATEMADRKNRYYRDLIAKLSPDDVLPGAIELIDEARQRGLKVSLASASKNA
                     REVLDRLGITDRFDAIADGNSVTAPKPAPDLFLFSARQLGLKPAECVVFEDASDGVDA
                     ALAAEMLTVGIGPDERVGQADVVLADGFASTNLDDIFNRIVRSREASPTGQRSAGRPS
                     RQGRRGSSALGPTRQPEGRAS"
     gene            complement(1319..2398)
                     /locus_tag="VMZ33_04590"
     CDS             complement(1319..2398)
                     /locus_tag="VMZ33_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16538.1"
                     /translation="MNRLQTTFRATTAGVLAALVVGAIVPGVVAAATRPVKFDVVMGY
                     SCISGRASDGATVELVWRQTDGTRKARASVPASASGGYWSYCSNDGDVVAIGDSFFAH
                     DGASSHAFVVPELTLVINRVDDVFKGVAPAGDYVRLICGYTNGFEPCQRTWSLRVNPE
                     GRWKFRPQWPVAGWQGMSVRWRSDDGDAVTVNARGPYVDVTIGSATVRGAARANGPVT
                     VVLRRAGSFDVAGTAVTTAGAYVGEFRTKFRDSNGNPVKVRVGDWITSDISPDVDFTV
                     PNVVASADASTDLVTGQCPDYSMFVEAVVMRSGYEDGDWDWPEEGGSFELDLSWPDLQ
                     AGESVRAHCYRADPGDWVGRLIVAQ"
     gene            complement(2626..>2953)
                     /locus_tag="VMZ33_04595"
     CDS             complement(2626..>2953)
                     /locus_tag="VMZ33_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16539.1"
                     /translation="AWKCGAYMPKKIEEALAAWRDAERRLQNAVDGDRAALVGEVERS
                     RSVFQQLSADHMMERMDALKKAEHRRKTAEPSTPSFHQAAKDEMVIATDIWDSARQSD
                     EDTPQR"
BASE COUNT          525 a         1022 c          907 g          499 t
ORIGIN      
        1 ctcctcggcg acgaaaccca gacccaggtc accctcgacg aattcctcac ggtcgcagat
       61 ggcatttaca tccaggagcc gcgcgaggat gacgtcgttg agcagttcga cggctacttc
      121 gggcggcgcg aagtggacct ctcggaatac gccgagcgct ccgagtcgat gcaggcgctg
      181 ctgggcatgg tcggcctcgc agagacgcag gtcatcaagc agcccgacgt cctgatgctt
      241 gccgctcttc tgccggagac gttcaccgac gcgcagctcg tcgccaacta cgactactac
      301 aacgctcgaa cagaccacag ccacggttcg tcgctcggac cgggcatcca ggcattgctt
      361 gcggcgcgcc ttggacgctt ggacgaggca tacgagcact tccagcgcgc cgcgcgcgtt
      421 gacctggccg acgttcgcgg caacaccgcg gacggaacac acgccgccag cgccggggcg
      481 ttatggcagg ccatcgtctt cggcttcgcc ggcgtcaatc ttgagggcga gaaagtcaga
      541 accaagccgc agctgccccc gcactggcgc cgactcaaat ttcggatcgc ccatcgcggc
      601 cggacggtcg atatcgacct tcgcccgaca ccgcagtcca cgcccggggc cgaggcaatt
      661 cgcggcctga tattcgacct cgacggcgtc gtcaccgaca ctgcggagtc gcactacctg
      721 gcctggcagc ggcttgccga cgaggagggc ctaccgttcg accgcgccgc caacgaggct
      781 ctgcgcggta tcagtcgccg ccagtcactg gcgctgctcc tcggcacgcg ctccgtctct
      841 gatgagcagg ccacggagat ggcggaccgc aagaaccgct actaccgcga cctgatcgcc
      901 aagctttcgc cagacgacgt gctgcccggc gcaatcgagc tgatcgacga ggcccgacaa
      961 cgcgggctga aggtctcgct ggcatcagcg agcaagaacg cccgcgaagt cctggaccgg
     1021 ctgggcatca ccgaccgctt cgacgccatc gctgatggca actccgtcac ggcacccaaa
     1081 ccggcaccgg acctattcct gttcagcgcc cggcagctcg gcctgaaacc tgccgagtgc
     1141 gtcgtcttcg aggacgcctc ggatggcgtg gacgcggcgt tggctgcgga gatgctgacc
     1201 gtcggcatcg gacccgatga gcgcgttggg caggccgacg tcgtgctcgc tgacggtttc
     1261 gcatccacaa acctggacga catcttcaat cggattgtgc ggtcacgcga ggccagtcct
     1321 actgggcaac gatcagccgg ccgacccagt cgccagggtc ggcgcggtag cagtgcgctc
     1381 ggaccgactc gccagcctga aggtcgggcc agctgagatc cagctcgaag gacccgccct
     1441 cctcgggcca atcccagtcg ccgtcctcat atccgctgcg cattacgacc gcctcgacaa
     1501 acatggagta gtcggggcac tgaccggtga cgaggtcggt cgacgcatcg gcactggcca
     1561 cgacgtttgg gacggtgaaa tccacgtcgg gcgaaatgtc agaggtgatc cagtcgccga
     1621 ccctgacctt gaccgggttc ccgttagagt cgcgaaactt ggtccggaac tcgccgacat
     1681 acgcgccggc ggtggtaacc gcggtgcccg ctacatcgaa cgaaccagct cgccgcagaa
     1741 cgacggtcac cggtccattg gcgcgcgccg cgccgcgaac ggtcgcgctg ccgatcgtca
     1801 cgtcaacgta tgggccgcgt gcattcactg tcaccgcgtc gccgtcatcg cttctccagc
     1861 gcaccgacat cccctgccag ccggcaacgg gccactgcgg ccgaaacttc cacctgcctt
     1921 ctgggttgac ccggaggctc caggtccgct ggcagggctc gaaaccgttc gtgtaaccgc
     1981 atatgagtcg cacgtagtcg cctgccggtg ccacgccctt gaacacgtcg tcaacgcggt
     2041 tgatcaccag ggtcagctcg ggaaccacaa acgcgtgact cgacgcgcca tcgtgggcga
     2101 agaaggagtc accgatggcg accacatctc cgtcattcga gcagtagctc cagtagccac
     2161 cggaggccga tgccggcacg gatgcccggg ccttgcgcgt gccgtccgtc tggcgccaca
     2221 ccagctcgac cgtagctccg tcgctggcgc gcccagaaat acaggaatag cccatcacga
     2281 cgtcaaactt gaccgggcgc gttgccgcgg ccacgacgcc cggaactatt gcgcccacca
     2341 caagtgcggc gagcacgcct gccgtagttg cccggaacgt ggtttgaagt cggttcattc
     2401 cagagccctc cgctcgacgg cgactgtgtt cggccccctg tcgctatcca gccagtagac
     2461 acgccagacc gaagaagcga caccgtggct gcacccatag tgctactgac gcgacactag
     2521 gtatacccac tcgttggaat aggtcgcctg gcatgtctgg atcggaagtc gagggtcgta
     2581 tgtccctctc cggcctcacg gatcgtgggc cgtgccattg gacggctatc tctggggggt
     2641 gtcctcgtca ctctggcgag cggaatccca gatatccgta gcgatgacca tctcgtcctt
     2701 ggccgcctgg tgaaacgagg gcgtggacgg ctcggcggtt ttgcgacggt gctctgcctt
     2761 cttgagggcg tccatgcgct ccatcatgtg gtccgccgag agttgctgga acacgctccg
     2821 ggaccgctcg acctccccca cgagcgcggc ccggtcgcca tcgactgcgt tctggagtcg
     2881 ccgttcggcg tcacgccagg cggcgagcgc ttcctcaatc ttcttcggca tgtacgcccc
     2941 gcatttccac gct
//
LOCUS       DATLCO010000187         2450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2503344, whole genome shotgun sequence.
ACCESSION   DATLCO010000187 DATLCO010000000
VERSION     DATLCO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503344"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VMZ33_04600"
     CDS             <1..652
                     /locus_tag="VMZ33_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HUR16540.1"
                     /translation="MANGARALADAYPNRFVLGMGVSHAPSVQMRGGDYANPLQQMTA
                     YLDAMETAQYGAPLPAEPAPLLLAALGPKMLELAAQRTDGAHPYFVPVEHTAVAREHL
                     GPGPTLATEVTAVLSTDRSAGLGIARSFAKHYLELPNYANNLKRLGWTDTDIATDGSD
                     RLIDAVIAIGDVDAIVSRVNAHLDAGADHVCLQVRTERSNDPAIAAFKELAGAFGI"
     gene            complement(649..1410)
                     /locus_tag="VMZ33_04605"
     CDS             complement(649..1410)
                     /locus_tag="VMZ33_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUR16541.1"
                     /translation="MCFDLDSQPPIPPIAGAAVDGQRVSLTAADGTRFAAFDAQPESP
                     TGAGVLIFPDVRGLHHFFEELALRFAERGINAVAVDYFARTAPNDDRGEGFDYQPHVA
                     QTQYEHLMADAKAARDRLVDHGVGQVFTMGFCMGGRLAFVAGTRPELDVTGAIGFYGW
                     PVGPGRGGTPAPADVAGEMTSSVLAIFGGADQGIPPEVVQQFADALDTAGVDNEVVTY
                     PDAPHSFFDRKADEFAGESADAWEKVLEFIQSRGR"
     gene            1713..>2450
                     /locus_tag="VMZ33_04610"
     CDS             1713..>2450
                     /locus_tag="VMZ33_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUR16542.1"
                     /translation="MTTESRPTTDMEPVYKPQEVEPRVYQRWLDADVFAPDGKGSRAD
                     WSRRPFVVTQPPPNITGSLHTGHALTTTVEDLMIRRARMQGLPTLWVPGVDHASIGAQ
                     VVLDKIIAKDGETRNSLGRDRYLERMWTFINETRALIGEQHQRLGASVDWGRLRFTMD
                     DGSAKAVRHAFKKLYDDGLAYRGEKLVNWCPGDLTSVSDLEVIGTPTKGTLWSVRYHL
                     VDDAGQPLPDATITVATTRPETILGDTA"
BASE COUNT          454 a          836 c          803 g          357 t
ORIGIN      
        1 catggccaac ggcgcgcggg ccctcgccga cgcgtatcca aatcgcttcg tactgggaat
       61 gggggtcagc cacgccccga gcgtgcagat gcgtggcggc gactacgcca accctctaca
      121 gcagatgacg gcctacctcg acgcgatgga gactgcccaa tacggtgcgc cactgcccgc
      181 cgagccggcg ccgctgctgc tcgcggcgct cggtccaaag atgctcgaac tggccgccca
      241 gaggaccgac ggcgcccatc cgtatttcgt ccccgtcgag cacacggcag tcgcgcgcga
      301 acacctggga ccagggccaa cgctggccac tgaggttacg gccgtcttga gcaccgatcg
      361 gtcggccggc ctcggcatcg cgcgatcgtt cgccaagcac tacctggagc tgccgaatta
      421 cgccaataac ctcaagcggc tgggctggac cgacactgac atcgccacag acggcagcga
      481 tcggctgatc gacgctgtga tcgcgatcgg agacgtcgac gccatcgtga gtcgggtcaa
      541 cgcccacctc gacgctgggg ccgaccacgt gtgcctgcag gtgcggacgg agcgctccaa
      601 cgacccagca attgccgcct tcaaggaatt ggcgggcgcc ttcggcatct agcggccgcg
      661 cgactgtatg aactcgagca ccttctccca ggcgtcagcc gactcccccg caaactcgtc
      721 ggccttgcgg tcgaagaagg aatgcggcgc gtcgggatag gtcacgacct cgttatcgac
      781 tccggccgtg tccagcgcgt ccgcaaactg ctgcacgacc tcgggcggaa tgccctgatc
      841 ggctccgccg aagatcgcca ggacagacga cgtcatttcg ccggcgacat cagccggtgc
      901 cggggtcccg cctcgaccag gcccgaccgg ccaaccgtag aagccgattg ccccggtgac
      961 gtccagctcg ggccgggtcc cggccacgaa cgccagccga ccgcccatgc agaagcccat
     1021 cgtgaaaact tgcccgaccc cgtggtcaac gagacggtca cgtgccgcct tggcgtcggc
     1081 catgaggtgc tcgtactgtg tctgggcgac gtgcggctgg tagtcgaagc cctcgcctcg
     1141 gtcgtcgttc ggcgcggtcc gggcgaagta atccaccgca acggcgttga tgccgcgttc
     1201 agcgaagcgc agcgcgagct cctcgaagaa gtggtgcagg ccgcgcacgt ctgggaagat
     1261 gagcacccct gcgcccgtgg gcgactcagg ctgagcgtcg aatgctgcga aacgcgtgcc
     1321 atcggcggcc gtcaaggaga cccgctggcc atcaacggca gcgcctgcaa tgggcggaat
     1381 cggcggttgg gaatcaaggt cgaagcacat gatcgggatg ctaaccttgg ggccgcgaca
     1441 gcgaccggta ggagtagcac cgctccagag ctgttcagag agatccggcc caaggctgaa
     1501 agccggacca gcatcggcgg tgcgaacgtc aaccgcgagt cgcgcggcac gcgcccgtta
     1561 catgcgcgga gagagtgctc ggaagcacgg ccgcgggatg accgcggtac gccggacact
     1621 gaaccagcag gtggtaccac gaccccgttc gtccgctgga cgatcggggc gattttgttt
     1681 tcaggcacga aacaacggga gctgacacac gcgtgacgac cgagagccgg ccgacaaccg
     1741 acatggagcc ggtctacaag ccgcaggaag tggagccgcg cgtgtaccag cgctggctcg
     1801 atgcggacgt gttcgccccc gacgggaagg gcagccgcgc ggactggtcc cgtcggccat
     1861 tcgtggtcac gcaaccaccg cccaacatca ctgggtcact ccacacgggc cacgctctca
     1921 cgaccacggt cgaggacctg atgatccgcc gcgcgcggat gcagggcctt ccgacgctgt
     1981 gggtgccggg tgtggatcac gcgtcgatcg gcgcccaagt cgtgctcgac aagatcatcg
     2041 cgaaggacgg ggagacgagg aactcgctcg gtcgcgaccg ctacctcgag cgtatgtgga
     2101 ccttcatcaa cgagacgcgc gcgctcatcg gtgagcagca ccagcgcctg ggcgcgtccg
     2161 tcgactgggg ccgcctgcgg ttcaccatgg acgacggctc cgcgaaggcc gtccgtcacg
     2221 cgttcaagaa gctgtacgac gacgggctcg cctatcgcgg cgagaagctc gtcaactggt
     2281 gtcctggaga cctcacgagc gtgtcggatc tggaggtcat aggcacgccg acgaagggca
     2341 cgctgtggtc ggtccgctac cacctcgtcg acgacgcagg tcagccgctc cccgacgcca
     2401 ccatcaccgt ggccaccact cgccctgaga cgatcctcgg cgacacggcc
//
LOCUS       DATLCO010000188         5209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10569743, whole genome shotgun sequence.
ACCESSION   DATLCO010000188 DATLCO010000000
VERSION     DATLCO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10569743"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /gene="pheT"
                     /locus_tag="VMZ33_04615"
     CDS             <1..1787
                     /gene="pheT"
                     /locus_tag="VMZ33_04615"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HUR16543.1"
                     /translation="IGKSPVRVQLRLSAAGMRPVSNAVDASNYVMLEMGKPIHVFDAG
                     SVSGGTIVVRPAQAGEKLETLDHVVRSLTPDDIVIADASGAIGIAGVMGGANSEVSSN
                     TTAAFVESAVFDPVSIRRTAFRYGLRSEASLRFEKGQESRLARLGADRTVQLLAQWAG
                     GRPAVGAIDTNPEDEMPRRVAFRPARISRLLGQNVTPAEMIEALAAVEIKATPAGDGE
                     ELPDGATVSESLVAIVPTHRRDIAIEADVAEEVCRVRGYDTLPPRLPETRLPAYRRDP
                     RQFKDALRDLLSGRGLSEVVTNGLIAPVDHERVGIPPHDAATIRVANPVTADHSELRR
                     TLLPGMLGVVARNERQRGPHLAIYEMGNVQEWRDGEPREVEVLGVMLAGNWRAASWAE
                     PARSASLEDLKGIVETLLARAGVGDISYGAADVRAGVEHPGRTALIASRAASSDEGAV
                     LGRVFEVDPRLGAAYDVRAEHVVFAILDVAVMARIAADRGVPKVRDVARLPVIERDIA
                     VVVGRDVSADRVAEVVRATAGSNLVTLELFDRYTGAPLEAGQVSLAYRLRFQPGDEAM
                     SEAAIDGAISAVTAALEHEVGGRIRSGS"
     gene            1777..2316
                     /locus_tag="VMZ33_04620"
     CDS             1777..2316
                     /locus_tag="VMZ33_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HUR16544.1"
                     /translation="MVVDIALVLLFLGALLIGILRGALRQLMTLGAWLVALVVAAYMR
                     EPVGDWIAAQTDYSREYVEMLGFGLAFIVLFALAVIVIQIGGRTVELIERPGFDEMLG
                     GVLGLAWAVMAITSLLIVLDTYYANPVRGSSELALVQDLNVELRDSSIGAALQRSAVP
                     GLGSVLGPLLPAAVRASLT"
     gene            2325..2918
                     /locus_tag="VMZ33_04625"
     CDS             2325..2918
                     /locus_tag="VMZ33_04625"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HUR16545.1"
                     /translation="MSRGFFARPTVDVARDLLGTLLHRDSSDGATVGRIVEVEAYDGP
                     TDRASHARAGLTRRTTPMFGEVGHAYVYLIYGMHNCLNVVAYDSSLAPAGAVLLRALE
                     PVAGSVLMGARRGRDGEPDSRLCSGPARLCQAMAVDRSLNAHDLTAGHGLSLTAGEAI
                     PDSQVATGPRIGVDYAGKWAEKPWRFWIVGHPSLSRR"
     gene            2915..>5209
                     /locus_tag="VMZ33_04630"
     CDS             2915..>5209
                     /locus_tag="VMZ33_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HUR16546.1"
                     /translation="MTDQRSQDLLEFPSIRARLAEHTAFGPSRRLAEALTPSSDPIVV
                     KRLLDETDEARDVAARRPDIGVGGARDIGQLILRARRRGRLSGSELLEILDTLVAAGR
                     VADALRNEKLPLIHELSRSIKTLPQLRGRLELSLDPAGELLDSASPALGGLRRAVRVA
                     YERLRTKLESLVHGSELSSALQEPLVTLRNGRYVIPVRADQRGRVKGIVHDQSGSGQT
                     VFIEPLVVVELGNAWREAQLAVESEEERILDELSALVDAQADPLEDTLAALATFDFWI
                     ARSRLSSELNGVRPDSSASGRVELLSARHPGLTGRVVPIDIRLGDTFRALIITGPNTG
                     GKTVALRTLGLLALMHQSGLHVPAAPGSRLPVFRDVFADIGDEQSIAQSLSTFSGHMR
                     SIVRIVDAAGPDCLVLLDELGAGTDPTEGSALAQALLDHFIRAGALIVATTHYAELKT
                     YAHNTPEASNASVEFDLETLSPTYRLQIGLPGTSHAFAIATRLGLPDGLVADARERLG
                     RAQQEFEETLVSIKEAQITAEEALARASDSEERARNARREAEEERRSARREREAALAN
                     ARTEAEKALAEVQSEIAAARDLLTRATLTESRLEESAERLRGTVGSLGGDEPEVDEPL
                     SPGSASSVHVGARVRTAAGWEGTVAEVDAEKGTATIAAGSMRINAALVELVAVAPAPQ
                     GGRRGDLGTSPGTVAQRPVMRAVPSSLDVRGARVEEAREMLDSYLDGAAVAGAPRVTI
                     IHGHGSGALRDALRAQLSGHPLIKS"
BASE COUNT          802 a         1757 c         1794 g          856 t
ORIGIN      
        1 agatcggcaa atcgccggtg cgcgttcagt tgcggctgtc cgctgcgggg atgcggcccg
       61 tctcgaacgc agtagatgcc tcgaactacg tcatgctcga gatgggcaag ccgatccacg
      121 tattcgacgc tgggtcggtg agcggcggca cgatcgtggt acgaccggcg caggcgggtg
      181 aaaagctcga gacgctcgac catgtcgtgc gttcactgac tccggacgac atcgtcatcg
      241 cggacgcgtc gggggccatc ggaatcgccg gcgtcatggg cggcgccaac agtgaagtca
      301 gctcaaacac gaccgcggcg ttcgtcgagt cggccgtgtt cgaccccgtc tcgatacggc
      361 gcactgcatt ccgctacggc ctgcggagcg aggcaagcct gcgtttcgag aagggccagg
      421 agtcccgact ggcccgtctg ggtgccgatc ggacagtcca gcttctggcg cagtgggccg
      481 gcggacggcc ggcggtagga gccatcgaca ccaaccctga ggacgagatg ccacggcgcg
      541 tggcgttccg accagcgcgg ataagccggc tgcttggcca gaacgtcact ccggctgaga
      601 tgatcgaggc cctggcggcg gtcgagatca aagccacgcc cgctggggac ggagaagagc
      661 tgccggacgg cgcgaccgtg agcgagtcgc tggtggcgat cgtgccgacc caccgccgcg
      721 acattgcgat cgaggccgac gtcgccgagg aagtttgtcg cgttcgcggg tacgacacgc
      781 tgccgccgcg gttgcctgag acccgcttgc cggcgtaccg gcgcgacccg cgacagttca
      841 aggacgcgtt gcgtgacctg ttgtccggcc gtggtctgag cgaagtggtc actaatggac
      901 tcatcgctcc cgtcgaccac gagcgcgtcg gcatcccgcc gcatgacgcc gccacgatca
      961 gggtcgccaa cccggtcacc gccgatcact ccgagctgag acggactctg ctgccgggaa
     1021 tgctcggggt cgtcgcccga aacgaacggc agcgcggccc tcacctcgcc atctacgaga
     1081 tggggaacgt ccaggagtgg cgcgacggtg agccacggga agtcgaggtg ctgggcgtga
     1141 tgctggccgg gaattggcgc gcggcatcct gggctgagcc cgctcggtcg gcctcgctcg
     1201 aagacctgaa gggcatcgtc gagacactgc tggcgcgagc gggcgtcggg gacatctctt
     1261 atggggctgc cgacgttcgc gcgggtgtcg agcatcccgg gcgaacagcg ctcatcgcct
     1321 cccgtgctgc gtcgagcgac gaaggagccg tactgggtcg cgtgttcgaa gtcgatcctc
     1381 ggctgggcgc cgcttacgac gtacgcgccg agcacgtcgt ctttgccatc cttgacgtcg
     1441 cggtgatggc gcgcatcgcc gctgatcgcg gcgtaccaaa ggtccgcgac gtcgcacgtc
     1501 tgccggtaat cgaacgcgac atcgccgtcg tcgttggccg cgacgtgtcc gcggaccgag
     1561 tcgccgaggt cgtccgcgca actgccggat caaacctcgt gacgctcgag ctctttgatc
     1621 gttacacggg cgcgccgctc gaggctggac aggtcagcct cgcttaccgc ctccggttcc
     1681 agcccggcga cgaggcgatg agcgaagcag ccatcgatgg cgcgataagc gctgtcacag
     1741 ccgctctcga gcacgaagtg ggaggacgca tccgcagtgg tagttgacat cgccctggtg
     1801 ctgctcttcc tcggcgcgtt gctgatcggc atccttcgcg gcgcgctgcg ccagctgatg
     1861 accctcggcg cgtggcttgt cgcgctcgtt gttgccgcgt acatgcgcga gccagtcggc
     1921 gactggatcg cggcgcagac cgactactcg cgcgagtacg tcgaaatgct tggcttcggt
     1981 ctcgcgttca tagtgctctt cgcgcttgcg gtgatcgtca tccagatcgg cggccgcacc
     2041 gttgagctga tcgagcgccc ggggttcgac gagatgctcg gcggcgtcct cggcctggcg
     2101 tgggcggtca tggctattac ttctttgctc atcgtcctcg acacgtacta cgcgaaccct
     2161 gtacggggct ccagcgaact tgccctggtg caagatctca acgttgagct gcgcgattcg
     2221 agcatcggcg ccgcgctgca gcgatcagcc gtgcctggcc ttggaagcgt tcttggaccg
     2281 ctgctgccgg cagcggtccg cgcgagcctg acctgaatga gtcgttgtcg cgcgggtttt
     2341 tcgcgcggcc gacggtcgac gtggcgcgcg atctcctggg cacgctgctt caccgcgact
     2401 cgtccgacgg agccacggtc ggcaggatcg tcgaggtcga ggcctacgac gggccgacag
     2461 accgggcgag tcacgcgcgc gccggcctca cgcggcggac gacgccgatg ttcggtgaag
     2521 tcggccacgc ctacgtttac ctgatctacg ggatgcacaa ctgtctcaac gtcgtcgcct
     2581 acgactccag cctcgcaccc gccggagcgg tcctgctccg ggcccttgag ccggtagcgg
     2641 ggagcgtgct catgggtgcc cggcgcggcc gggacgggga gcccgattcg cgcctgtgct
     2701 ccggaccggc acgcctgtgc caggccatgg ctgtcgacag gtcactcaac gctcacgacc
     2761 tgacggccgg tcacgggcta tcactgacgg cgggggaggc cataccggac tcgcaggtcg
     2821 ctaccggccc acggatcggc gtcgactacg cggggaagtg ggcagagaag ccgtggcgct
     2881 tctggatcgt cggccacccg tcgctgtctc gtcgatgacc gaccagcgca gccaggacct
     2941 gctggagttc ccgtcgatcc gtgcgcgcct cgctgagcac accgcgttcg gcccatcgcg
     3001 ccgcctcgcc gaggcactca cgccgtcgtc cgaccccatc gtcgtcaagc ggctgctcga
     3061 tgagaccgac gaggcgcggg atgttgctgc tcgccgccct gacatcggag tgggcggggc
     3121 gcgtgatatc ggccagctca tccttcgcgc tcgccgccgc ggccggttgt ccgggtcgga
     3181 gctgctggag atcctcgaca cgctggttgc cgccggacgc gtggccgacg cgttgcgcaa
     3241 cgagaaattg ccgctcatcc acgagctgag tcgttcgatc aaaacgctgc ctcagctccg
     3301 ggggcgcctg gagctgagtc tcgatcccgc cggggagctg cttgatagtg cctcgccggc
     3361 gctgggtggc ctccgccgcg cagtgcgcgt ggcctatgag cggctacgca cgaagctgga
     3421 gtcgctggtc cacggctctg agctttccag cgcccttcag gagccgctcg tcaccctgcg
     3481 caacggtcgc tacgtgatcc ctgtccgtgc cgaccagcgc ggtcgggtca agggcatcgt
     3541 ccacgaccag tcggggagtg gccagaccgt attcatcgag ccactcgtcg tcgttgagct
     3601 cggcaatgcc tggcgggagg ctcagctggc ggtcgagtcg gaggaggaac ggatcctcga
     3661 cgagctctcg gctctggtcg acgcccaggc cgacccgctc gaagacacgc tggcagcgct
     3721 ggcgacgttc gacttctgga tcgcgcgctc acggctgtcg agcgagctca acggcgttcg
     3781 gcccgacagc tccgcctccg gccgtgttga gctgctatcg gcccggcatc cgggcttgac
     3841 tggccgagtc gtaccgatcg acatccggct cggagatacc ttccgggcgc tgatcatcac
     3901 tggtccgaac acgggcggca agacggtcgc gctgcggacg ctcggactcc ttgcgctgat
     3961 gcaccagtcg gggctgcatg ttcctgccgc gcccggctca cgcctgccgg tgttccggga
     4021 tgtcttcgcg gacatcggcg acgaacagtc gattgcccag tcgctgtcga cgttctccgg
     4081 ccacatgcgc tcgatcgtgc gcatcgtgga cgcggccggc ccagattgcc tggtcctgct
     4141 cgacgagctt ggcgcgggca ccgaccccac ggagggttcc gccctggccc aggccctcct
     4201 cgaccacttc atccgcgccg gtgccctgat cgtcgcaacg actcattacg ccgagctcaa
     4261 gacctacgcc cacaacacgc ccgaggcctc caacgcgtcg gtcgagttcg acctggagac
     4321 cttaagcccg acataccggc ttcagatcgg cctgccgggc acctcgcacg ccttcgcgat
     4381 cgcgactcgc ctgggtctgc ccgacgggct ggttgccgat gcgcgcgagc ggctcgggcg
     4441 agcgcagcag gagttcgagg aaacgctcgt ctcgatcaaa gaggcgcaga tcacggccga
     4501 ggaagcgctc gcccgtgcat ccgactccga ggagcgcgcc cgcaacgcgc gccgcgaggc
     4561 agaggaagaa cggcgcagtg cgcggcgcga gcgtgaggcg gcgctggcga acgcacggac
     4621 ggaggctgag aaggccctgg cggaagtcca gtcggagatc gctgcggcgc gcgacctctt
     4681 gacccgcgca acactcactg agtcacggct cgaggaatcg gccgaacgcc tgcgcggcac
     4741 cgttggctcg ctgggtgggg acgagccgga agtggacgaa cccctctcgc ccggctcggc
     4801 gtcaagcgtc catgtcggag cccgcgtacg gacagctgcg gggtgggaag ggacagtcgc
     4861 cgaggtcgac gccgagaagg gcactgcgac gatcgctgcg ggctcaatgc gcatcaacgc
     4921 cgcgttagtc gagctggtag cagtcgcacc cgcgccacag ggcggacgcc gcggcgacct
     4981 tggcacaagc cctggcacag tcgcgcagag gccagtgatg cgcgccgtcc cctcgagcct
     5041 ggacgtccgt ggcgcccgtg tcgaggaagc acgcgagatg ctggactcgt acctcgacgg
     5101 agcggcggtc gccggtgcgc cgcgggtcac gatcatccat ggccacggtt ctggcgccct
     5161 ccgcgacgcc ctgcgcgcgc agctgagcgg ccatccactg atcaagtcc
//
LOCUS       DATLCO010000189         3038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3122410, whole genome shotgun sequence.
ACCESSION   DATLCO010000189 DATLCO010000000
VERSION     DATLCO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3122410"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VMZ33_04635"
     CDS             complement(<1..331)
                     /locus_tag="VMZ33_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16547.1"
                     /translation="MAGLKYRGLDPRTRPLRWALFLLVIGAVLYATAPGAAQTVAGWF
                     GTSAESLPWYASRALGFLGLWGLTASVVYGLLLSTGILDSVSHRVVSLTLHQDLSAIS
                     IALVALHG"
     gene            complement(319..1248)
                     /locus_tag="VMZ33_04640"
     CDS             complement(319..1248)
                     /locus_tag="VMZ33_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HUR16548.1"
                     /translation="MTDTSFDFVSWTAPQMGGRTEVTIAAEQHRRAVAGVAARRAGQR
                     IAAWADRLTRFDERSDLSLLNASAESSVAVRPMLGATLRWAATASERTAGIVDPTLLH
                     ARLAAETGSRFSLSPDASGWAIRAAGRTTLVDRTRPTRFDLDGIAKGWLADRAVDLLS
                     EWSAVAVDADGDICVRLDAGMEWLIDVADPRASGAHPLATLRLTGGQSWSQTYGIATS
                     GTTVHRWQIGHGHATHHLIDPRTGVPADTDVVQATVLAPTAREAEALAKAAIIHGSHD
                     ALLFLNRSAALTAILLLETGDVAAMPGIDRWLA"
     gene            complement(1250..1741)
                     /locus_tag="VMZ33_04645"
     CDS             complement(1250..1741)
                     /locus_tag="VMZ33_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16549.1"
                     /translation="MNAPAPAARFAKPDPRPMRWAFGASAVAAVSILTVGFVKPDFAT
                     TDDQAVVNDSVAEQAAGATNRSSRARVNRVTRYVYLERGERAPRGATVIDADQAPGRR
                     QQPRGDQPEDPRVATNTRPTNARPANARPANNQPQNNDRPAADPQPRPEQPPRTTTHQ
                     SGG"
     gene            complement(1888..2367)
                     /locus_tag="VMZ33_04650"
     CDS             complement(1888..2367)
                     /locus_tag="VMZ33_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16550.1"
                     /translation="MVHKLALILGGAGVAGVFALALVLGGSPFALPASAESGVAALAS
                     GSAPTNAPEPTATDGRKTRTVVDTVFVRPAPTAEATADPTAAPANPTAQPKADSNSGR
                     NERRADNNDDSVRAGSNRGTDHDDSFDDDSHHDHDDEHGDDHTGHGRGDDDDREDHD"
     gene            2544..>3038
                     /locus_tag="VMZ33_04655"
     CDS             2544..>3038
                     /locus_tag="VMZ33_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16551.1"
                     /translation="MTRLLLVEDEPRIASAIKRVLLAEHYTVDATEDGETALAFLGEH
                     AYDVVILDRLLPGISGDDVLRTMRGRANHTPVLMLTALSALEHRVEGLDAGADDYLPK
                     PFAFSELLARLRALTRRPREVATERLSIGGIMLEPDRHQVSVDGKSELLSAREYALLG
                     YLIRN"
BASE COUNT          461 a          973 c         1094 g          510 t
ORIGIN      
        1 cgccgtgtag cgccacgagc gcgatcgaga ttgcgctcag gtcctggtgc agtgtcaggc
       61 tcacgacgcg gtgcgagacc gagtcgagaa ttcctgtcga gagcagcagc ccgtaaacga
      121 cggaggcggt aagcccccag agcccgagaa acccgagggc gcgactggcg taccagggca
      181 ggctctctgc gctggtgccg aaccagccgg cgacggtctg cgctgcgccc ggcgcggttg
      241 cataaagcac ggcgccgata accagcagga agagcgccca acgcagcggc cgcgttcgcg
      301 ggtcgagccc gcggtacttc aggccagcca tcggtcgatc ccgggcatgg ccgccacgtc
      361 acccgtttcg agcaagagga tggccgtcag cgccgccgag cggttcagga aaagaagcgc
      421 gtcgtgtgac ccgtggatga tggcggcctt ggccagtgct tcggcttcgc gcgcggtggg
      481 cgccagcacg gtcgcctgca cgacgtccgt gtcggcgggc acgccagtcc gagggtcgat
      541 gagatggtgt gtggcatgtc catgaccgat ctgccagcga tggaccgtcg tgcccgaagt
      601 cgcgatcccg taggtttgcg accagctctg gccgccggtg agtcgcaggg tcgccagcgg
      661 gtgcgcgcct gaggcgcgcg ggtcggcaac gtcgataagc cactccatgc cggcgtcgag
      721 tcgcacacag atgtcgccat cggcgtcgac cgcgacggcc gaccattccg ataggagatc
      781 aaccgctcga tcagccagcc agcccttggc gatgccgtcg aggtcgaacc gcgttggccg
      841 cgtccgatcg acaagagttg tccggccggc tgctcggatg gcccagccgc tggcgtcagg
      901 cgaaaggctg aacctggagc ctgtctcggc cgctaggcgt gcatggagca gggtcggatc
      961 aacgatgccg gcggtgcgct cggaggcagt cgcggcccaa cgaagggtgg cgccgagcat
     1021 gggacgcaca gccacgctgc tttcggccga cgcattgagc aggctcaggt ccgatcgctc
     1081 gtcgaagcga gtcaagcggt cagcccacgc tgctatgcgt tggccggcgc gccgggcggc
     1141 cacacccgcc acagcgcgcc ggtgttgttc cgcagcgatc gtgacctcgg ttcgcccccc
     1201 catctgcggc gcggtccagg acacgaagtc gaaagatgtg tctgtcacgt cagccgcctg
     1261 actggtgtgt ggtcgtccgt ggcggctgct cgggacgagg ctgcgggtcg gctgcaggtc
     1321 gatcgttgtt ctgtggctgg ttgttggccg gccgggcgtt ggccggccgg gcgttggtcg
     1381 gccgcgtgtt ggtcgcgacc cgagggtcct ccggctggtc gccgcgaggc tgctggcgtc
     1441 ggccgggcgc ctgatcggcg tcgatgaccg ttgctccgcg aggcgcacgc tcgccgcgct
     1501 ccaggtacac gtaacgcgtc acgcggttga cgcgagcccg ggagcttcgg ttggtggcgc
     1561 cggccgcctg ctcagcgacg gagtcgttga cgactgcctg gtcgtccgtg gtggcgaagt
     1621 cgggcttcac gaaaccaacc gtcaggatgc tgaccgccgc gacggcgctg gcaccgaacg
     1681 cccaacgcat cgggcgcgga tcaggcttgg cgaaacgtgc cgcgggtgcg ggggcgttca
     1741 atggcgccgc tggtgcggcg atgctcctgg gcgaggcggc gattggtgcc ggggcggcga
     1801 tcggagcggc gcccgcggcg tccttggtcg gtagtggacg acctgcagtc acagaccggc
     1861 gtggcgggag gggcggcagg tcggccatca gtcgtggtcc tcccggtcgt catcatcacc
     1921 gcggccgtgg ccggtgtgat catcgccgtg ctcgtcgtcg tggtcgtgat gcgagtcgtc
     1981 gtcgaacgag tcgtcgtgat cagtccctcg gtttgagccg gcgcggaccg agtcgtcgtt
     2041 gttgtcagca cggcgttcgt tgcgaccact gtttgaatcg gccttgggtt gcgcggttgg
     2101 gttcgcggga gcggcggttg ggtcagccgt cgcttcggca gttggggccg gtctgacgaa
     2161 tacggtgtcg accactgttc gggtcttccg cccgtcggta gcggtcggct cgggtgcatt
     2221 ggtcggcgca gaaccgctgg cgagtgccgc caccccggat tcggccgatg cgggcagggc
     2281 aaacggcgac ccgccaagta cgagagccag ggcgaagaca ccggcaacgc cggcgccacc
     2341 gagtatgaga gcgagcttgt ggaccattcg tgtccctccc gatatgtgga ctgacaacac
     2401 gggaaagact cgcaacggtg tttgaggcca tcctttgacc aggattggag ttcgcacacc
     2461 ctggcgccac cctcgatgag cgttctcaaa gaaacgcgcc tcaaccgttg cgcgcacgcg
     2521 tgaaccgggg gttaattgca gccatgacac gcctgctact ggtcgaggac gagccacgca
     2581 tcgcatctgc gatcaagcgc gtgctcctcg ccgagcacta cacagtcgac gcgaccgagg
     2641 acggcgagac ggcactcgcg ttcctgggcg agcacgcgta cgacgtcgtg atcctcgacc
     2701 gtctgctgcc cggtatcagt ggcgatgacg tgctgcggac gatgcgtggc cgcgcgaacc
     2761 acactccggt cctgatgctg accgcactct ctgccctgga gcatcgcgtc gaggggctcg
     2821 acgccggtgc cgacgactac ctgcccaagc cattcgcctt ttcggagctg ctggcccgac
     2881 tccgggcact cacgcggcgt ccccgtgagg tggccacgga gcggctgagc atcggcggga
     2941 tcatgcttga gcctgatcgc caccaggttt ccgtcgacgg gaagtccgaa ctgctgtcgg
     3001 cgcgcgagta cgcgctgctc ggctacctga tccgcaac
//
LOCUS       DATLCO010000190         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3128014, whole genome shotgun sequence.
ACCESSION   DATLCO010000190 DATLCO010000000
VERSION     DATLCO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3128014"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..>2040)
                     /gene="glgX"
                     /locus_tag="VMZ33_04660"
     CDS             complement(207..>2040)
                     /gene="glgX"
                     /locus_tag="VMZ33_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HUR16552.1"
                     /translation="DGGRNDTDSAPFMPRSIVINPFFEWGTDRLLRRPLHETVIYETH
                     VKGFTRLHPEIPEDTRGTFLGLAHPAAIRYLTGLGVTAVELLPVHQFVGSGTADDSGV
                     GNYWGYNSIGFFAPHNGFSRSVGRGEQVAEFKQMVAALHEAGIEVILDVVYNHTAEGN
                     HMGPTLSFKGIDNAAYYHLVGDDKRHYMDYTGTGNTLNAGHPYSLQLIMDSLRYWVQE
                     MHVDGFRFDLAVALGRQERDVERSAAFFNLIQQDPVVSQVKLIAEPWDTGEGGYQVGN
                     FPPLWSEWNGKYRDTVRDYWRGQPRTLSELGYRLTGSSDLYETSARRPHASINFISAH
                     DGFPLADLVSYNDKHNEANGEDNRDGEKDNRSWNHGVEGPTDDAEINALRARQRRNFM
                     TTLLLSQGVPMILGGDEIGRTQTGNNNAYAQDNEISWYDWPNADAQMLEFTRGLITLR
                     RVHPVFRRRRWFLGRQIHGEQLTDIAWLGPDGNLMGEGDWNDASSQAIGLYLDGDNLG
                     VDQRGERITDTTFYLLLNSSPDDVVFFLPNSTWGGAWRVVLDTLTGEPPDLVADPPAS
                     ALLPAPEGGEDVSDASSVDPDKLFEAASDITVTSRSVVLLERVS"
BASE COUNT          370 a          629 c          655 g          386 t
ORIGIN      
        1 ccgcgggtcc acgacgtcgt agccgtgggt ggacccgggc gccgcttgca ggtacggcga
       61 cgagtagaag tgactgacgc ctaagcggtg gaggtaatcg gcgattccgg ctgcagcctc
      121 gaagccgaaa gccggctgaa gttgcacccg gtaggtggca cgtggcaccc gggtggaaac
      181 agctgacccg ttcttcgtct cgcctatcag gagacgcgct ccaggagcac gaccgacctc
      241 gacgtcacag tgatgtccga cgcggcctcg aaaagcttgt ccgggtctac ggagctcgca
      301 tcggaaacat cctcgccacc ctcaggtgcg ggcaacaagg ctgaggctgg cgggtcggct
      361 acgagatcgg gcggctcgcc cgtcagggtg tctagaacga cccgccaggc gcctccccac
      421 gtggagttcg gcaggaaaaa gacgacgtcg tcaggactgg agttcagcaa gagatagaac
      481 gtcgtgtcag tgatgcgctc accgcgctgg tcgacgccca ggttgtcacc gtcgaggtaa
      541 aggccgatcg cctggctcga cgcgtcattc cagtccccct cacccatcag gttgccgtcc
      601 ggacccagcc aggcaatatc ggtcaactgc tcgccgtgta tctggcggcc caggaaccac
      661 cgtcgccgac ggaacacagg gtggacgcgc cgcaacgtga tcagtccgcg cgtgaactcg
      721 agcatctggg cgtcggcgtt cggccagtcg taccaggaga tctcgttgtc ctgagcgtag
      781 gcgttgttgt tgcctgtctg agttctgccg atctcgtccc cgccgaggat catgggcacg
      841 ccctggctga gcagcagcgt ggtcatgaag ttccgccgct ggcgcgcccg gagcgcattt
      901 atctcggcgt cgtcggtcgg gccttccacg ccatggttcc acgaccggtt gtccttctcg
      961 ccgtcgcgat tgtcctcacc gttggcctcg ttgtgcttgt cgttgtacga aacgagatcg
     1021 gccaacggaa aaccgtcatg ggcgctgatg aagttgatgc tcgcgtgtgg acggcgcgct
     1081 gatgtctcgt agaggtccga cgatccggtc agtcggtagc caagctcgct cagcgttcgc
     1141 ggttgacccc gccagtaatc gcggacggtg tcgcggtatt tgccgttcca ctccgaccac
     1201 aagggcggaa agttgccgac ctgatagcca ccctcacccg tgtcccacgg ctcggcgatg
     1261 agcttcacct ggctgacgac ggggtcctgc tgaatcaggt tgaagaaagc cgcgctgcgc
     1321 tcgacatccc tctcctgtcg acccagggcg acggccagat caaagcggaa gccatcaacg
     1381 tgcatttcct ggacccagta gcgcaggctg tccatgatca gctgcaggga ataagggtga
     1441 ccggcgttca acgtgttgcc ggttccggtg tagtccatgt agtgacgctt gtcgtcccca
     1501 accaggtggt agtacgcggc gttgtctatg cccttgaagc tgagtgtcgg gcccatgtga
     1561 ttgccctcag ccgtgtggtt gtagacgacg tcgagaatga cctcgatgcc tgcctcatgc
     1621 agcgcggcga ccatctgctt gaactccgcg acctgctcgc cgcggccgac ggaccgcgag
     1681 aaaccgttgt gcggcgcgaa gaagccgatg gagttgtagc cccagtaatt gccgacgcca
     1741 ctgtcgtcgg cggtcccgct gcccacgaac tggtgcaccg gcagaagctc gacagcggta
     1801 acaccgagtc cggtcaggta gcggatcgct gccggatggg cgaggcccaa aaacgttccc
     1861 cgggtgtctt ccggaatttc cggatgaagc ctcgtaaagc ccttgacgtg agtctcgtag
     1921 atgacggttt cgtggagtgg ccggcgaagc agccggtcag taccccactc gaagaacgga
     1981 ttgatgacga tggagcgcgg catgaacggc gcactgtcgg tgtcgttgcg acccccatca
//
LOCUS       DATLCO010000191         3656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10577175, whole genome shotgun sequence.
ACCESSION   DATLCO010000191 DATLCO010000000
VERSION     DATLCO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10577175"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VMZ33_04665"
     CDS             complement(<1..503)
                     /locus_tag="VMZ33_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16553.1"
                     /translation="MSVLDAPASKPRSVERQASALVRAWGGRGDGLDAMVERVGGVDQ
                     VGIEERVASLTRRSIKKQSKLWALDLAIRMMDLTTLEGKDTEGKVRALCAKGMRPDPT
                     DPTIPSVAAICIYPALIPVAKKALAGSTVHVASVSTGFPSGQTYLDVKLLETRQAVEA
                     GADEIDMV"
     gene            567..848
                     /locus_tag="VMZ33_04670"
     CDS             567..848
                     /locus_tag="VMZ33_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR16554.1"
                     /translation="MTVADDRLLDQVLHAIADETRRRLLSHVSDNPGLTTAQLAELIP
                     TMTRWGVMKHLEVLRAAGLIQAMSEGRNTRHFVERSALAPVKRYLESAG"
     gene            complement(827..1897)
                     /locus_tag="VMZ33_04675"
     CDS             complement(827..1897)
                     /locus_tag="VMZ33_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16555.1"
                     /translation="MGRVPRAEEEVHLLLGQELASLERQLDHGSSIEPESDALQEGPI
                     EVGSAIDIGSNSVHLLVAVVGEGWLEPLRDTSELLGLGDVVDRDGSIPKDSRKAVVDV
                     LADYVGTANRSQSEHMTLLGTEPLRRASNADTLAAEIFERTGESLHVLSEHGEALLTF
                     IGVTRGVQPRAPLIVVDIGGGSTEVATWLPGQPLETRSIPLGSARLSKATIEHDPPTD
                     SEMNALAGAALDATADLAPLLHRADLLAGARAIFVGGTATNIARLGTLSRDDLARDRA
                     TLTNMPTTTVTTRYNVKPRRARQLAAGVAIVDAILERFELDTAEASDASLRDGAIIAR
                     CRLGEEWPERLDELVGYPALSR"
     gene            complement(1801..2574)
                     /locus_tag="VMZ33_04680"
     CDS             complement(1801..2574)
                     /locus_tag="VMZ33_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HUR16556.1"
                     /translation="MGMRPTLLLIALLVAALALPGTVAGVNLQEASGGSLGAPATNSA
                     AEGMDSAQPGSAALSSWSGRFSVFYPRTHSVQKTDYYCVPASIQMMLNIIDHESDKSR
                     AGQDRYFRYAQDHSRYPILDNGADPQGWAEALQHWGAGNYSVGVHSTMQASLKAAAKR
                     MRMTGKPVGLIVWGNRGGHAWVMTGFAADADPAATDSYRVSSIQAMGPLYPYGTIGGR
                     AFDPGPREWVEYPELKKKFTYYWVKNSPAWNGSWITVLP"
     gene            2731..3171
                     /locus_tag="VMZ33_04685"
     CDS             2731..3171
                     /locus_tag="VMZ33_04685"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HUR16557.1"
                     /translation="MPLSTGMDPLNRSSFTSGREYLVPSRVHADRAASTLELEWPDGH
                     ATIYDFETLRWLCPCAYCRGEAGMPGWLDSNPTLSPAQTQLTDIALVGNYAIQPTWAD
                     GHHTGFYTFLRLREDCPCAEDAERRARERGDTGDSAQSAARRLG"
BASE COUNT          627 a         1205 c         1174 g          650 t
ORIGIN      
        1 accatgtcga tctcgtcagc accggcctcg acggcctgac gggtttcgag gagcttcacg
       61 tcgaggtacg tctggccgga cggaaagccg gtcgacacgc tcgcgacgtg gaccgtgctg
      121 ccggcaagcg ccttcttcgc caccgggatg agggctgggt agatgcagat ggcggcaacg
      181 ctcggaatgg tcgggtcagt cggatccggg cgcatgccct tggcacacaa cgcccgaacc
      241 ttgccctcgg tgtccttccc ctcgagggtg gtcagatcca tcatccgaat cgcgagatcc
      301 agggcccaca gcttcgactg cttcttgatt gaccgacggg tcaggctggc gacacgttcc
      361 tcgatgccca cctggtcgac accgccgacg cgttcgacca ttgcatccag tccatcgccg
      421 cggccacccc atgcgcgcac gagcgcactt gcctggcgct caacagagcg tggcttgctg
      481 gcgggggcgt cgaggacact catcgcactg atcgtacacc ggtgtgtctt gactttgtgg
      541 ctcagtcaat gtgactatgc ggtcacatga ccgttgcaga cgaccggttg ttggaccagg
      601 tactccacgc catagccgat gaaacccgcc ggcgccttct ttcgcacgtc agcgacaacc
      661 ctggactgac gaccgctcag ctcgccgaac tcatccccac gatgacgcgc tggggagtga
      721 tgaagcacct tgaggtgctg cgcgccgccg gattgatcca ggcgatgtcc gagggacgca
      781 acacacgtca cttcgtggag cgctcggcgc tcgcgccggt gaagcgctac cttgagagcg
      841 ccgggtagcc gaccagctcg tcgagacgct ccggccactc ttcgcccaat cggcaccggg
      901 cgatgatcgc gccgtcgcgc agactggcgt ctgatgcttc ggccgtgtcc agctcgaagc
      961 gctcgaggat cgcgtccacg atcgccactc cggcggcgag ctgacgcgcg cgacgcggct
     1021 tgacgttgta gcgagtggtc accgtggtgg tcggcatgtt tgtcaacgtc gcccggtcgc
     1081 gcgcgagatc gtcccgactc aacgtaccca gccgcgcaat gttcgtggcc gtgccgccaa
     1141 caaagatggc ccgcgcaccc gccaacaggt ctgccctatg taacaggggc gccaggtcgg
     1201 ccgtcgcgtc cagtgccgcg ccggcgaggg cgttcatctc ggaatcggtc ggcgggtcgt
     1261 gctcgatggt ggctttcgac agccgggcgg acccaagggg gatgctgcgc gtttcgagcg
     1321 gctgacccgg tagccaggtg gccacttccg tggacccgcc accgatatcg accacgatga
     1381 gcggcgctct cggctgcacc ccgcgcgtca cgccaatgaa cgtcagcagc gcctcgccat
     1441 gctcggacag gacgtggagt gactctccgg tgcgctcgaa tatctcggcc gcgagagtgt
     1501 cggcattgct ggctcgccgc agcggctccg tgcccaggag tgtcatgtgc tcggattggg
     1561 atcgattggc cgtgccgacg tagtccgcca acacgtcgac caccgctttg cgggaatcct
     1621 tcgggataga gccgtcccgg tcgaccacat cacccaagcc gaggagctcg ctcgtgtcgc
     1681 ggagcggctc aagccagccc tcgccgacca ccgctaccag gagatggacg ctgttgctgc
     1741 caatgtcgat ggccgacccg acctcgatcg ggccttcctg gagcgcgtcg gactccggtt
     1801 ctatggaaga accgtgatcc agctgccgtt ccaggctggc gagttcttga cccagtagta
     1861 ggtgaacttc ttcttcagct cggggtactc gacccattcc ctggggcccg gatcgaacgc
     1921 cctgccaccg atcgtgccgt acgggtagag cgggcccatg gcctgaatag agctgactcg
     1981 gtagctgtcg gtcgcggccg ggtcggcatc ggcggcgaaa ccggtcatga cccacgcgtg
     2041 cccgcccctg ttgccccaca cgatcaagcc gacgggcttg cccgtcatgc gcatccgctt
     2101 ggctgcagcc ttcagcgagg cctgcatggt cgagtgcacg ccgacgctgt aatttccggc
     2161 gccccagtgc tgcagcgcct ccgcccagcc ctgcgggtca gccccgttgt cgaggatcgg
     2221 gtagcggctg tggtcctggg catagcggaa gtaacggtcc tggccggcac ggctcttatc
     2281 ggactcgtga tcgatgatgt tcagcatcat ctggatgctg gccggcacgc agtagtagtc
     2341 cgtcttctgg actgagtgcg ttcgcgggta gaacacgctg aacctgcccg accagctcga
     2401 cagggccgca gaacctggct gtgcgctgtc catgccttcg gcggcagaat tggtcgcagg
     2461 cgcgccgaga gaaccaccac tcgcctcttg caaattgact ccagcgacgg tcccgggcag
     2521 cgcgagtgca gccaccagga gcgcgatcag tagcagcgtg ggcctcatcc ccatttcggc
     2581 ctgtgtacct ctcgttgtct cggtataggt gtctagtcgg cgttcacggc agcccgactc
     2641 gtataaccct accgatctcg gcacaggatt gcgccgggca cgaacgtgcc gggtcgaaca
     2701 aaccaatatc ggtttgcaaa tcccggtttc gtgccactat cgacgggaat ggacccgttg
     2761 aacagaagct ccttcacgag cggccgcgag tacctcgtgc cgagtcgcgt ccacgctgac
     2821 cgtgctgcgt cgactctcga gctcgagtgg cccgacgggc atgccacgat ctacgacttc
     2881 gagacgctcc gctggctgtg cccctgcgct tactgccggg gagaggcggg aatgcccggg
     2941 tggctggact cgaacccgac actcagccca gcgcagacac agctgaccga tatcgcgctg
     3001 gtgggcaact acgccattca gccgacgtgg gccgacggcc accacacagg tttctacacg
     3061 ttcctgcggc tgcgcgagga ctgcccctgc gctgaggacg cggaacggcg cgcacgggag
     3121 cgtggcgata ccggcgactc tgctcaatcg gcggcgcgcc gcctcggctg accgcgggca
     3181 cagacagctc tcgacaaaac catgatccca tcgatatcgc tcacatcatt agccgggagc
     3241 aaaggtcggt aattgcgagc tctgaggggc gtccaccgct tgattgtgcc gcgcggagca
     3301 cagatgactg tgcggaagcg ctccggaatc gggcgtggcg cagggagccc cgccaattcg
     3361 ggcatccaaa agatccgcca cacgctgacg taatgatgca ggcgatatcg gaacctgcat
     3421 gcttcagaac ccgccggttc gacggggtgc gttcgctgag cgagcaacgc gtcgggcgag
     3481 tgactgacgg accccgactg acggagattt gacccgctga gggagtcgaa tccgcgtctg
     3541 agggcccgtg tagccgcccg tcactacggc tcggcggaca gaatgctcgc tgagcgagtc
     3601 gatatgccag gcggcgcgga taagtacgtt cggccgattg cggcgcggtg cgctca
//
LOCUS       DATLCO010000192         5303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2525518, whole genome shotgun sequence.
ACCESSION   DATLCO010000192 DATLCO010000000
VERSION     DATLCO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2525518"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VMZ33_04690"
     CDS             <1..266
                     /locus_tag="VMZ33_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HUR16558.1"
                     /translation="PRVLYTPLYMPATRTQIYLTEEQRRLLDARGRRTGASLAQMIRE
                     AVNEYVSDDAADLAAALDETFGSMPDLEIPDRLEWDRIADPGR"
     gene            complement(595..1137)
                     /locus_tag="VMZ33_04695"
     CDS             complement(595..1137)
                     /locus_tag="VMZ33_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HUR16559.1"
                     /translation="MVKDRENVPEGLPPFDAWQDAAREGQDAREVVSESETAAGQTSG
                     EVSIAAELLQAVIDAAREGVPNEACGLLVAPAYASEGGTPTRYVGVRNAAESPYRYMI
                     DPQDQLKVWMELEDAGEVPWAIVHSHVASLAVPSATDIGLAFFPDSLYVICSLAGDVP
                     TVRAWSIREQVVSEVTLAVI"
     gene            complement(1131..2063)
                     /locus_tag="VMZ33_04700"
     CDS             complement(1131..2063)
                     /locus_tag="VMZ33_04700"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HUR16560.1"
                     /translation="MTAYPSLIETIGHTPLVEISRFNTNPRVRLLVKLEGANPTGSVK
                     DRVARYMVEALERDGRLSAHSVILEPSSGNTGIALAMICRLRGYSLTIVMPDNVTRER
                     RQMLSIFGASVVDSPGELGSNGAVDLARRMAAEDSRYVLADQYANPANPKAHYETTGA
                     EIVADCPEISVFVAGLGTGGTLMGAGRRLREHNPSIKIYAAEPMPGEKVQGLRSLEDG
                     FVPEILDPSALDGRFLVTTEESIETLRRLMQTEGIFSGVSSGGVLAVAARIADGMESG
                     VVVALLADGGWKYLSEDLWRRDPDEEELETLNLW"
     gene            complement(2060..2362)
                     /locus_tag="VMZ33_04705"
     CDS             complement(2060..2362)
                     /locus_tag="VMZ33_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HUR16561.1"
                     /translation="MTRVRIPPVLRIHLDGQKEAEATGGTVGELLRDLVSRHPGLGEQ
                     LFTENGEMHRFVNVYVNGQDVRYLGVLETPVSDRDQVIILPAMAGGAGAGDVSGEA"
     gene            complement(2359..3513)
                     /gene="moeB"
                     /locus_tag="VMZ33_04710"
     CDS             complement(2359..3513)
                     /gene="moeB"
                     /locus_tag="VMZ33_04710"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HUR16562.1"
                     /translation="MRPELPMARGPVREVMPAEAEEMLAQGAALIDTRETEEWQAGHL
                     TGATLIAPREVVGRIEQVVPDKSKPVVIYCAAGARSMRAAMQLGAMGYQNVVSVAGGI
                     IQWKTEGRPWQMPDDSPSIARNPRYSRHLIMPEVGPKGQAQLSQSKVLFIGAGGLGSP
                     GLLYLAAAGIGTIGIVDFDVVDISNLQRQVVHADDRIGMKKTKSAEMTIRGLNPDVKV
                     VAHEEMLADDNVDRLIAGYDIVIDGTDTFETRYTLNDAAVRARIPVVHASVFRFEGQL
                     SVFVPFEGPCYRCLYPTPPPPELAPGCSVAGVLGVVPGVMGLLQATEAIKVLLSIGDP
                     LVGRLMIWDALDGTFSEVALKRDPNCPACGDGARSAAANTIGERQLAPAG"
     gene            complement(3503..3901)
                     /locus_tag="VMZ33_04715"
     CDS             complement(3503..3901)
                     /locus_tag="VMZ33_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HUR16563.1"
                     /translation="MDDLYRDEILEHYRNPHNFGTLDTPTAVKEGANPLCGDRITLML
                     GIDDDGNVQDVAFTGRGCAISQASASMLTDEIKGKPLSEIARMGKDDVLENLGIEISP
                     ARLKCAMLSLETLQSAVSDRVVWQGETDAA"
     gene            complement(3908..5155)
                     /locus_tag="VMZ33_04720"
     CDS             complement(3908..5155)
                     /locus_tag="VMZ33_04720"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HUR16564.1"
                     /translation="MAIVQALDPVAIRADFPIFEREFRGRRMAYLDSAATALKPRVVA
                     EAVDGYLRNYSANVHRGIYEIGEEATAAYEGARTKIANFLGASDAREIVMVRNATEAI
                     NIVAYAWGRKNVGKADTVVVTEMEHHSNIIPWQMLTQERDADLEFVPFDDDGRLIQES
                     YEALLRTKPKLVAFTAVSNGIGTINPVREMVDKAHEAGALVLIDGAQAAPHEPVSVKD
                     LDADFFVFSGHKTLGPPGSGALWARREILEEMPPFMGGGEMIREVHLRHTTFNDVPYK
                     FEAGTPDIAAAIGLGAALDYLSGLGMENVRAHERDLVAYGLEVLPREVKGIRIHGPKT
                     AEDRAGILTFNLADAHPHDVATLLDREAIAIRAGHHCTMPLHQRLGEESSARASMYVY
                     SDREDIDRLAEALVKVERLFARS"
BASE COUNT          898 a         1757 c         1718 g          930 t
ORIGIN      
        1 aacctcgtgt gctgtatact ccgctgtata tgccagcgac ccgcacccag atctatctga
       61 ccgaggagca gcgccgcctg cttgatgcgc gagggcgtcg gacaggtgcg tccctcgcgc
      121 agatgatccg agaggctgtg aacgagtacg tgtcagacga cgcagcagat ctggcggccg
      181 cgctggacga aaccttcgga tcgatgccgg atctggagat acccgaccgt ctcgaatggg
      241 atcgaattgc ggaccctggt agatagcgac gttctggtcg atcacctgcg gggatacgca
      301 cggtttgaga cccgaggcga catcgttcat gtgtcgtcga tcaccagagc agaactgttc
      361 gcgggacgca acgcggacga gaaaggcgtg cgcgctcttc tctcgtcgat gacagagttg
      421 ggtgtcgacc aaaccctcgc ggagagagcc ggtcggattc gtcggaccac gggcatagga
      481 cttgcggatg ccttgatagc tgcaaccgcg ctcgaacatc gtcttgccct agttaccaga
      541 aacgtgcgcc acttcgaagt cgtccgcggc cttcgcctgc gctagaacgc gtcgtcagat
      601 gacggcgagg gtcacctcgg agacgacctg ctcgcggatt gaccacgcgc gaaccgttgg
      661 gacgtcgccg gccaaggagc agatgacata gagcgagtcg gggaagaacg ccaagccgat
      721 gtccgtcgca gacggcacgg ccagtgacgc gacgtgggag tggacgatgg cccacggcac
      781 ctcgccggcg tcctcgagct ccatccagac cttgagctga tcctgcgggt cgatcatgta
      841 gcggtacggc gactcggccg cgttgcgcac gcccacgtag cgggtcggcg tgccaccctc
      901 gcttgcgtag gccggcgcga ccagcagacc gcacgcttcg ttgggcacgc cctcccgcgc
      961 ggcgtcgatc acggcttgaa gcagctctgc cgcgatgctc acctcgcccg atgtctgacc
     1021 ggcggcagtc tcggactccg agacgacttc tcgcgcgtcc tgaccctcgc gcgctgcatc
     1081 ctgccaggcg tcgaaggggg gcaatccctc gggcacgttc tcacgatctt tcaccacagg
     1141 ttcagcgtct ccagctcctc ttcgtcggga tcgcgccgcc acagatcctc cgacagatac
     1201 ttccatccac cgtcggccag aagggcaacg accacgcccg attccatccc atccgcgatc
     1261 cgtgccgcga ccgccagcac accacccgac gacacgccgg agaaaatccc ctcggtctgc
     1321 atcaaccgac ggagagtttc gatcgactcc tccgtcgtga ccaggaaccg cccatccagc
     1381 gccgacggat cgaggatctc gggcacgaac ccatcctcaa gtgaccgcag accctgcacc
     1441 ttttcgccgg gcatcggctc ggccgcatag atcttgatcg atgggttgtg ctcccggagc
     1501 cgccgaccgg cacccatcaa ggtcccgccc gtgcccaggc cggcgacaaa gacagagatc
     1561 tcaggacagt cggccacgat ctcggcaccg gtcgtctcgt aatgcgcctt tgggttggcg
     1621 gggttggcgt actggtcggc caggacgtag cgggaatctt cggccgccat ccgccgcgcc
     1681 aggtcgaccg ccccgtttga gcccagctca cccggcgagt cgactactga ggcgccaaag
     1741 atcgaaagca tctggcgccg ttcacgggtg acgttgtcgg gcatcacgat cgtcaacgaa
     1801 tagccgcgca gccggcagat catggcgagg gcaatgcccg tgttgcccga ggacggttcg
     1861 aggatcaccg agtgcgcgga cagccgaccg tcgcgctcca gcgcctcgac catgtagcgc
     1921 gcgacgcggt ctttgaccga gcccgtgggg ttggcaccct ccaacttgac gaggagccgg
     1981 acacggggat tcgtgttgaa ccgcgagatc tcgaccagcg gcgtgtggcc gatcgtctcg
     2041 atcagtgacg ggtaggcggt cacgcctctc cggagacgtc ccctgcgccg gctccgccag
     2101 ccatagccgg caggatgatc acctggtcgc gatcggagac aggcgtctca agcacgccca
     2161 ggtagcgcac gtcttggcca ttcacgtaga cgttcacgaa gcgatgcatc tcgccgttct
     2221 cggtgaagag ctgctcaccc aggcccggat gtcggctgac gaggtcccgc aacagctcgc
     2281 ccaccgtccc gccggtcgcc tcagcctctt tctggccatc gaggtgtatc cggagaactg
     2341 gtgggatccg gacccgggtc accctgccgg cgcgagctga cgctcgccga tcgtgtttgc
     2401 ggcagcggac cgggcgccat cgccgcatgc cgggcagttc ggatcgcgct tgagggcgac
     2461 ttcgctgaac gtgccgtcga gcgcgtccca gatcatcaac cggccgacca gcgggtcgcc
     2521 gatgctgagc aggaccttga tcgcttcggt cgcctggagt aagcccatga cgcccggcac
     2581 gaccccgagt acaccagcca cggagcagcc gggcgcgagc tcgggcggcg gcggcgttgg
     2641 ataaaggcag cgatagcacg gcccctcgaa ggggacgaag acgctcagct ggccctcgaa
     2701 ccggaaaacg gaggcatgca cgacggggat gcgcgcgcgg actgccgcgt cgttcaacgt
     2761 gtagcgcgtc tcgaacgtgt cggtgccgtc gatgacgatg tcgtaaccgg caatcagccg
     2821 gtcgacgttg tcgtcggcga gcatctcctc gtgggcgacg accttcacgt cggggttaag
     2881 cccgcgaatc gtcatctcag ccgacttggt tttcttcatg ccgattcggt cgtcagcgtg
     2941 gaccacctgg cgctgaaggt tggagatgtc gacgacgtcg aagtcgacaa ttccgatagt
     3001 cccgattccg gcagccgcca gatagagcag gcccggtgag ccaagaccac ccgccccgat
     3061 aaacagcacc ttcgattgcg aaagctgcgc ctgtcccttg ggcccgacct cgggcatgat
     3121 caggtggcgc gagtagcgcg ggtttcgggc aatcgacggc gagtcgtccg gcatctgcca
     3181 cggccggccc tctgtcttcc actggatgat cccaccggca accgagacga cgttctggta
     3241 gcccatggcg ccgagctgca tggcggcgcg cattgagcgc gcgcccgcgg cgcagtagat
     3301 gacgaccggc ttggacttgt caggcacgac ctgctcgatg cgaccgacta cctcgcgcgg
     3361 cgcgatgagc gttgcgccgg tcaggtgacc ggcctgccac tcctccgtct cgcgcgtgtc
     3421 gatgagcgcc gcgccctgag cgagcatctc ctcagcttcg gcgggcataa cctcgcgaac
     3481 agggccacgt gccatgggga gctcaggccg catcagtttc tccctgccac accacgcggt
     3541 cgctgacggc cgactggagg gtctcaagcg acagcatcgc gcacttcaag cgtgccggac
     3601 tgatctcgat cccgaggttc tcgaggacgt catccttgcc catccgggca atctcggaga
     3661 gcggcttgcc cttgatctcg tcagtcagca tgctcgccga ggcttgactg atcgcgcagc
     3721 cgcggccggt gaaggccacg tcctggacgt tgccgtcgtc atctatgccg agcatcagcg
     3781 tgatgcggtc accgcagagc gggttcgcgc cttccttgac tgccgtcggc gtgtcgaggg
     3841 tgccgaagtt gtgggggttc cggtagtgct cgaggatctc gtcgcgatat aggtcgtcca
     3901 tgtgtcccta gctgcgcgcg aagaggcgct cgaccttgac gagtgcctcc gcgagacggt
     3961 cgatgtcctc gcggtcggag tagacataca tgctggcccg ggcgctcgat tcctcgccca
     4021 gtcgctggtg gagcggcatc gtgcagtggt gcccggcacg gatcgcgatg gcctcacggt
     4081 cgagcagcgt agcgacatcg tgtggatgcg cgtcggcaag gttgaaggtc aggatgccgg
     4141 cgcgatcctc tgcggtcttg gggccgtgga tgcggatgcc cttgacctcg cgcgggagca
     4201 cttcgaggcc gtaggccacg aggtcgcgtt cgtgggcacg aacgttctcc atgcccagcc
     4261 cgctgaggta gtcgagcgcc gccccgaggc ctatcgccgc ggcaatgtcc ggcgtgccag
     4321 cttcgaactt gtaggggacg tcgttgaagg tcgtatgacg caggtggacc tcgcggatca
     4381 tctcgccgcc acccatgaag ggcggcatct cttcgaggat ctcgcgacgt gcccaaagcg
     4441 cgcctgaacc tggcggtccg agcgtcttgt ggcccgaaaa gacgaagaag tccgcgtcaa
     4501 ggtccttgac actgaccggc tcgtggggcg ctgcctgggc accgtcgatg aggaccagcg
     4561 cgccggcctc gtgcgccttg tcgaccatct cgcgcaccgg gttgatagtc ccgatgccgt
     4621 tgctcaccgc cgtaaaggcc acgagcttgg gcttggtcct gagcagagcc tcgtatgact
     4681 cctggatgag ccgcccatcg tcgtcgaagg gcacgaattc caggtccgcg tcacgctcct
     4741 gggtcagcat ctgccacggg atgatgttgg agtggtgctc catctcggtc accacgaccg
     4801 tgtcggcctt gccgacgttc ttgcggcccc acgcgtaggc gacgatgttg atcgcctccg
     4861 tcgcgttgcg aaccatcacg atctcgcgcg catcgctggc gcccaggaag tttgcgatct
     4921 tcgtccgggc gccctcgtag gcggccgtgg cctcctcgcc gatctcgtag ataccgcgat
     4981 gcacgttggc gctgtagttg cgcaggtagc catcaactgc ctcggccacg acgcgcggct
     5041 tcagggcggt cgcggcggag tctagatacg ccatccggcg gccgcggaac tcgcgctcga
     5101 aaatcgggaa gtcggcccgg atggctacgg ggtcgagcgc ctggacgatg gccatcagtg
     5161 cggggctcct tcgtcgtttt cggccgaaac gatgccctcg cgctcctcgt ccgcgtcggg
     5221 caggccggcg tcgcgaagga tcggggcata gccctcttcc tccagacgca gcgccaggtc
     5281 ccgtccgccc gacgcaacga tcc
//
LOCUS       DATLCO010000193         3324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1036461, whole genome shotgun sequence.
ACCESSION   DATLCO010000193 DATLCO010000000
VERSION     DATLCO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036461"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VMZ33_04725"
     CDS             <1..219
                     /locus_tag="VMZ33_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16565.1"
                     /translation="GPMGVQVAVPANSYNSHIYRVRVTMFWYAADGSVQGKARHEVDY
                     YGKIVDGEEWYGETDGCIGHPAVHGDGP"
     gene            complement(234..452)
                     /locus_tag="VMZ33_04730"
     CDS             complement(234..452)
                     /locus_tag="VMZ33_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16566.1"
                     /translation="MSEELHPEPVFGLRGQYENIWHLAAAERWSDGTVIGHRTYCGRN
                     YPNTEERPDPAELNEVCSDCTAQVANRH"
     gene            580..1119
                     /locus_tag="VMZ33_04735"
     CDS             580..1119
                     /locus_tag="VMZ33_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16567.1"
                     /translation="MAATIALAAAALPMAAGSVAAGGAQPPDHGAGVRCKYTTDADTN
                     WAFRTPLRRIAVLPPEIYARTARQTVGWRFLVNRLIDEGENPQWQVTYPSSLQRGSAT
                     STQPANFDTLRVGVTVPRNVEDRRNVTYTVTLKMYWFRADGSVASKASILLDDYTQYL
                     NGENWGGDDHCPGIVWAAV"
     gene            complement(1142..1852)
                     /locus_tag="VMZ33_04740"
     CDS             complement(1142..1852)
                     /locus_tag="VMZ33_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16568.1"
                     /translation="MKHAAAIASTGAEQLALQIDSRPDAALPYRVKPMTPVIGDAPFD
                     DRDWFFEPWWPGSSALAYIDGSHVRLQTSHMADPTIAFPELAGISSQFASDRLILEGT
                     LLVLDADGRPDGDLLRQRLARPASRPGVGAFVVSDLLYQGGQSLMSQRFEERRARLMR
                     DLTDGETCVISRGLRGDGSTLAEAVASMGVAEISARLLSAPYRSGTQDAAWLRLPVVE
                     APVTPTRPLLALLQRLPL"
     gene            complement(1849..2784)
                     /locus_tag="VMZ33_04745"
     CDS             complement(1849..2784)
                     /locus_tag="VMZ33_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HUR16569.1"
                     /translation="MPRAMWRGAIQFGLVTVPVKLYLATEPRAGLSFNMLHKDCGLRI
                     QMKTHCPEHGEILRSDTMRGYEYSKGQYVVIEEGDLESVPLKTMRSIEIELFVDASRE
                     AHGVTFVKQAYYLEPEAIGRKAFYLLKSVLAEEGLSAIAKIVLKDREALASINPYSNT
                     MLLSTLYWPDEVRTTGELELPQEDFEFKPAEIQMAQQLVNAMKGEFTADNYKDEYRQA
                     LLAVIEAKIAGQPAERVAAVETPTKIADLMSVLEASVAAAREARATRESGSTGTAAEP
                     TAAKKSRRKAEPTPVAAAAESAAEDAKPARRRKVA"
     gene            2888..>3324
                     /locus_tag="VMZ33_04750"
     CDS             2888..>3324
                     /locus_tag="VMZ33_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR16570.1"
                     /translation="MDPQAKTLTAGRLWGALSDSVGRAMVGAGEPLRLLASALLVGGH
                     VLVEDVPGVGKTLLAKSFAKSLGLSFSRVQGTPDLLPSDVTGSSVLDGGDFRFIQGPI
                     FSNVVLFDEINRATPRTQSALLEAMQEKQVSVDGQTRALPRPFV"
BASE COUNT          559 a         1095 c         1098 g          572 t
ORIGIN      
        1 ggcccgatgg gcgtgcaagt ggcggttccg gccaactcgt acaacagcca catctaccgg
       61 gtccgcgtga cgatgttctg gtacgccgct gacggcagcg tccagggcaa agccaggcac
      121 gaggtcgatt actacggaaa aatcgtcgac ggcgaggagt ggtatggcga gacggacggc
      181 tgcatcggac atccggccgt ccacggcgat gggccgtagc gccgaggagc aaatcagtgg
      241 cggttagcca cctgggcggt gcaatccgag cacacctcgt tcaactcggc aggatcgggc
      301 cgctcctcag tgttggggta gttgcggccg cagtacgtgc ggtgcccgat gactgtgccg
      361 tcgctccagc gctcagccgc ggcgaggtgc cagatgttct cgtattgccc gcgcaggccg
      421 aagacaggct cgggatgaag ctcctcactc acgccgtgag tctacggcca gtcggatggt
      481 gaaaccaacg gagacgaacg cagcggagga gagaacgcca tggccaggtt gaccccagag
      541 gcatcgcgac gaagtcgggc ttcccggaat ccgtggcgga tggccgcaac cattgccctc
      601 gccgcggccg cccttccgat ggccgccggc agcgtcgcgg caggtggtgc tcagcccccg
      661 gatcacggcg ctggcgttcg ctgcaaatac acgactgacg ccgacaccaa ctgggctttc
      721 aggacgccct tgcgacgaat cgcggttttg ccgccggaga tctacgccag gacggcccgc
      781 cagaccgttg gctggcgttt cctggtcaat cggctgattg atgagggcga gaacccccaa
      841 tggcaagtca cgtaccccag ctccctgcag aggggatcgg cgacctcgac gcagccggcc
      901 aacttcgaca cgctgcgcgt cggcgtgacg gtgcccagaa acgtcgagga cagacgcaat
      961 gtcacctaca cggtgacgct caagatgtac tggtttcgcg ccgatggcag tgtcgcttca
     1021 aaggcctcga tcctgctcga tgactacacc cagtacctca acggcgagaa ttggggaggg
     1081 gacgaccact gcccgggcat cgtttgggcg gccgtctagg ccgaccgcat ccttagcacg
     1141 cctagagggg cagcctctga agcagggcca gcagcgggcg cgtgggcgtc accggcgcct
     1201 cgacgacggg cagccggagc cacgcggcgt cctgcgtgcc cgatcgatag ggcgccgaca
     1261 gcagacgggc tgagatttcg gccacgccca tcgacgccac ggcctcagcc agcgtggacc
     1321 catccccgcg caggcctcgg ctgatgacgc acgtctcgcc gtcggtcagg tctctcatca
     1381 ggcgtgcgcg ccgttcctcg aacctttgtg acataagtga ctggccaccc tggtagagca
     1441 ggtccgaaac gacgaacgcg ccgacgcccg gccgcgatgc cggtcgcgcc aggcgctggc
     1501 gaagcaggtc accgtcgggg cgcccatcgg cgtcgagaac cagaagcgtg ccctcgagga
     1561 tgagccgatc gctcgcgaac tgcgaagaga tccctgccag ctcaggaaac gcgatcgtcg
     1621 gatcagccat gtgcgacgtc tgaagtcgga catggcttcc atctatgtaa gccagcgccg
     1681 aactgcccgg ccaccacggc tcgaagaacc agtcccggtc gtcgaacggc gcgtcgccga
     1741 tcacgggcgt catcggcttg acgcgatacg ggagcgcggc atcgggccgc gagtcgatct
     1801 gaagggccag ctgctcagcc cccgttgagg cgatagccgc cgcgtgcttc aagcgacctt
     1861 gcgccgacga gccggcttcg catcctcggc agcggactca gccgcggcgg cgacgggtgt
     1921 cggctcggcc ttgcggcgcg acttcttggc tgcggtcggc tcagcggcag ttcccgtcga
     1981 accggactcg cgcgtcgcac gcgcctcacg tgcggccgcg acgctcgctt cgagcacgct
     2041 catgaggtcg gcgatcttgg tcggcgtctc gacggcggcg actcgctccg ccggctgtcc
     2101 ggcgatcttg gcctctatta ccgcaagcag cgcctggcgg tactcgtcct tgtagttgtc
     2161 cgccgtgaac tcgcccttca tcgcgttgac gagctgctgc gccatctgga tctcagccgg
     2221 cttgaactcg aagtcctcct gtggcaactc gagctcgccc gttgttcgga cctcatcagg
     2281 ccaatagagg gtcgacagca gcatggtgtt ggagtacggg ttgatcgatg ccagcgcctc
     2341 gcggtccttg agcacgatct tggcgattgc ggacaggcct tcctccgcca gcacagactt
     2401 gagcaggtag aaggccttgc gcccaatggc ctccggctcc aggtagtaag cctgcttgac
     2461 gaaggtgacg ccgtgcgcct cacggctggc gtcgacgaat agctcgatct ctatcgagcg
     2521 catggtcttg agcgggaccg actcgaggtc gccctcctcg atgaccacgt actggccctt
     2581 gctgtactcg tatccgcgca tggtgtcgct gcgcaggatc tcgccgtgct cagggcagtg
     2641 cgtcttcatc tggatgcgca ggccgcagtc cttgtgcagc atgttgaagc tcaagccggc
     2701 ccgaggctcg gtggccaggt acagcttgac cggcacggtc accagtccga actggatcgc
     2761 gccccgccac atggcgcgtg gcatcgtcgt tctccttatt gcgcggtgct cgtccccgca
     2821 cgaggagttt accccgaccg aacgggcgtt cgaaggtcga ttggcgtgca cgacttacga
     2881 tgcgcgcgtg gatccccaag cgaagaccct gaccgcggga cggctgtggg gggcgctgtc
     2941 cgactcggtc ggtcgcgcca tggtcggtgc cggcgagcct ctgcggttgc tggcgtcggc
     3001 actgctcgtg ggcggccacg tcctggtcga ggacgtgcca ggcgtcggca agacgctgct
     3061 tgccaagtcc ttcgccaagt cgctcggctt gagcttctcg cgcgttcaag gcacgccgga
     3121 cctgctgccc agcgacgtga cgggatcgtc cgtgctcgac ggcggcgatt tccggttcat
     3181 ccagggaccc atcttcagca acgtggtgct cttcgacgag atcaaccggg ccacgccacg
     3241 gacccagtcg gcacttcttg aggcgatgca ggagaagcag gtctccgtcg acggccagac
     3301 ccgtgcgttg ccgcggccat tcgt
//
LOCUS       DATLCO010000194         4120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_291904, whole genome shotgun sequence.
ACCESSION   DATLCO010000194 DATLCO010000000
VERSION     DATLCO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291904"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VMZ33_04755"
     CDS             <1..198
                     /locus_tag="VMZ33_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HUR16571.1"
                     /translation="AREMLDSYLDGAAVAGAPRVTIIHGHGSGALRDALRAQLSGHPL
                     IKSWRAGERGEGGDGATIVEF"
     gene            complement(254..3178)
                     /locus_tag="VMZ33_04760"
     CDS             complement(254..3178)
                     /locus_tag="VMZ33_04760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUR16572.1"
                     /translation="MQTNRDGWDPNEPSRSDSRRGRRKPGVRRQLAVAVPMFVFALMS
                     MIGIGAFFAVVAVFASYSQGLPETNELENLQFISESIVYDRTGEVELARFNGGEQREP
                     VTFEQIPPILIDATTSTEDRSFWTNSGVDPLAIMSAGLDALRGRERGASTITQQLVRQ
                     RLLDPDLVRDPSRTAERKLKEIIQSVRVTDAYPGDEGKRRIITAYLNQNYYGNGSYGI
                     KAAARSYFGVEDLNDLTLGQVALLAGLPQSPSSYDLVRNSVVGEDGSLYVPLNERVPI
                     VFRRNEILRQMAEEPSRLVLTQGEYTRSELLAAIDEPIILTPQEPELRQWLAPHFIWA
                     LRDELAMRLCAGEETCPELEQGGLRVYSSLDYDLQQTAEKWVEAATILPQQSDPEAYA
                     AQIGVPYERWMRRLATLEVNNGAMIAMDYQTGEIVAYVGSASYYRDDLASPQFQPQFD
                     VLGDGWRQPGSAFKPFNYVTGINDGTMTASSMFMDVTTTFDNSNGYTPKNYDLLERGP
                     LRMRSALQWSLNVPAVKAQVINGVDHVFDQARRFGMSFQTERPQAGLSLTLGTEVTHP
                     RDVAVAFGTLGNGGVKVGYTHILRITNGAGEDLVPVYTPTAQDTVVSPQAAYVMTDIL
                     QSNTDPNQNPIWGEFALRGPNGDRRPATIKTGTSQDANDLVAFGYVPSTDDAGRQAGE
                     YSLVVGAWVGNSDGSPVLTEENPVLSTDVAAPMWHGFLQEVTSSWPVRGFARPPGIVE
                     ADVDAWSGMAPTEFTRQTVREVFIEGTVPPADNTKVALQVVGTGEQEVPPGEETEAQP
                     RFLLWAEGCAPPETHGFLALENVEAGHPDWQAANLDWINRARTGGPGTAGGPDPEERT
                     RTSYTFNRGYTPYGRSWGAPFAPTESCVPGATPAPSLSLEPSPSLSPSLEITPEPTFE
                     VTLPPPPTPTATPTNPPTEVPPPPTNPPPPTDPPPPPPTDPPPPPVEVTLPPAESIAP
                     TP"
     gene            3261..>4120
                     /gene="tyrS"
                     /locus_tag="VMZ33_04765"
     CDS             3261..>4120
                     /gene="tyrS"
                     /locus_tag="VMZ33_04765"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HUR16573.1"
                     /translation="MTVTNDGGPALLNELTWRGLIAEKSQGLDERLTRGPISGYIGFD
                     ASARSLHVGHLLQVFMLTHLQQAGGTPFVIIGGGTGMIGDPSGKSSERQLLNDEQIRS
                     NAAAIQAQLERFVDFSPGPNQAQMVDNREWLASWGLLEFLRDVGKHFSVPYMLAKDSV
                     QARLADGMSFTEFSYMTLQSADFLQLYRTRGVEMQMGGADQWGNITAGLELIRRVVGR
                     EEGEESAAFGLCSPLLLTRDGKKMGKSEKGAVMLDPSMTSPYDFYQYWLNDDDSLAVQ
                     HLRWLTLMTA"
BASE COUNT          662 a         1299 c         1418 g          741 t
ORIGIN      
        1 gcacgcgaga tgctggactc gtacctcgac ggagcggcgg tcgccggtgc gccgcgggtc
       61 acgatcatcc atggccacgg ttctggcgcc ctccgcgacg ccctgcgcgc gcagctgagc
      121 ggccatccac tgatcaagtc ctggcgcgcc ggggaacgtg gcgaaggtgg cgacggagcg
      181 acgatcgtcg agttctagcc cggtcgcgtg gccgacttcg atccggtgaa gcccgcagct
      241 ggtcggggag tagctagggc gttggtgcga tcgactcggc cggtggcagg gttacctcga
      301 ccggcggtgg cggagggtcc gtcggcggcg gaggcggagg gtccgtcggc ggcggagggt
      361 tcgttggcgg tggtggcacc tccgtgggcg gattggtcgg cgtcgcggtc ggcgtgggcg
      421 gcggcggtag cgtcacttcg aacgtcggct cgggcgtgat ctccaggctc ggtgagagac
      481 tgggtgaagg ctcgaggctg agcgacggcg cgggcgtcgc acccggtaca caggactcgg
      541 tcggagcgaa aggagcgccc cacgatcgcc cgtagggcgt gtagccgcgg ttgaaggtgt
      601 aactcgtccg tgtgcgctcc tccgggtcgg gcccaccggc cgtgcccgga cccccggttc
      661 gcgcgcgatt gatccagtcg aggtttgcgg cctgccagtc gggatgaccg gcttcaacat
      721 tttcaagcgc caggaagccg tgggtctcag gcggggcgca accctcagcc cacaacagga
      781 agcgtggttg ggcctcagtc tcctcacccg gcggaacctc ctgctcgccg gtcccgacga
      841 cctgaagcgc aaccttggtg ttgtccgcgg gcgggacggt gccctcgatg aagacttcgc
      901 gcacggtttg gcgcgtgaac tcggtcggcg ccatgccgct ccaagcgtcc acgtcagcct
      961 cgacgatgcc cggtggccgc gcgaagccgc gcaccggcca agagctcgtc acctcctgga
     1021 ggaagccgtg ccacatgggc gcggccacgt ccgtggacaa aacggggttc tcttccgtta
     1081 gaaccggcga gccatcgctg ttgccaaccc acgcgcccac cacgagcgag tattcgccgg
     1141 cctgcctgcc ggcatcatcg gttgatggca cgtagccgaa ggcgaccagg tcgttggcgt
     1201 cctggctcgt tcccgtcttg atcgtcgcgg gccgacggtc gccgttcggc ccgcgcaggg
     1261 cgaattcacc ccaaattggg ttctggttcg gatcggtatt gctctggagg atgtccgtca
     1321 tcacgtaggc cgcctgcggc gagaccacgg tgtcctgcgc ggtgggcgtg tacacgggca
     1381 cgaggtcctc gccggcaccg tttgtgatcc gcaggatgtg cgtgtagccg accttcactc
     1441 cgccattgcc aagcgttcca aacgccacgg ccacgtcgcg tgggtgggtc acttccgtgc
     1501 ccagcgtcag cgacaggccc gcctgcggcc gctccgtctg gaaggacatg ccgaagcgcc
     1561 gcgcctggtc gaagacgtgg tcgacgccgt tgatgacctg cgccttgact gcaggaacgt
     1621 tgagggacca ctgcagggcg ctgcgcatcc gtagcggacc ccgctcgagc aagtcgtagt
     1681 tctttggcgt gtagccgttc gagttgtcga acgtcgtagt gacgtccatg aacatggacg
     1741 aggccgtcat cgtgccgtcg ttgatgccgg tcacgtagtt gaatggcttg aacgctgatc
     1801 cgggctgccg ccaaccgtcg ccgagaacgt cgaactgagg ctggaactgg ggcgatgcga
     1861 ggtcgtcgcg gtagtagctg gcgctgccga cgtacgcgac tatctctccc gtctggtagt
     1921 ccatcgcgat catcgcgccg ttgttgacct cgagcgttgc caggcggcgc atccagcgct
     1981 cgtaggggac gccgatctgc gcggcatacg cttcgggatc cgactgttgg ggcaggatcg
     2041 tggctgcctc gacccatttc tcggcggttt gctgaaggtc gtagtcgagc gatgagtaga
     2101 cgcgcagccc gccctgctcc agttcagggc acgtctcctc gccggcgcac agtcgcatgg
     2161 cgagctcgtc gcgcagtgcc cagatgaagt gcggcgcgag ccactgacgc agctcgggct
     2221 cctggggtgt gaggatgatc ggctcgtcga tggccgccag cagctccgaa cgcgtgtact
     2281 cgccctgggt gagcaccagc cgtgacggct cctcggccat ctggcggaga atttcgttgc
     2341 gtcggaagac gatgggcaca cgttcgttca gcggaacgta gagcgagccg tcctcaccga
     2401 cgactgagtt gcgaacgagg tcataagacg acggtgactg cggcaggccg gcgagcaagg
     2461 ccacctggcc gagtgtcaga tcgttgagat cctcgacgcc gaagtagctg cgcgctgccg
     2521 ccttgatccc gtacgagcca ttgccgtaat agttctggtt gagataggcg gtgatgatcc
     2581 gacgcttgcc ctcatcacct gggtaagcgt cggtcacgcg aaccgactgg atgatctcct
     2641 tgagctttcg ttccgctgtc cggctggggt cgcgaaccag gtcagggtcg agaagccgct
     2701 ggcggaccaa ctgctgggta atcgttgagg cgccacgctc gcggccacgg agggcgtcga
     2761 gcccggccga catgatcgcc agggggtcga cgccggagtt ggtccagaag gaccgatctt
     2821 cggtcgaggt ggtcgcgtcg atcaggatcg gcgggatttg ctcgaacgtg acgggctcgc
     2881 gctgctcacc gccgttgaag cgcgccagct ccacctcacc cgttcggtcg tagacgatcg
     2941 attcagagat gaactgaagg ttctcgagct cattggtttc gggcaggccc tggctataag
     3001 acgcgaacac tgcgacgacc gcgaagaagg cgccgatgcc gatcatcgac atcagcgcga
     3061 acacgaacat ggggacggct accgcgagtt ggcgcctgac acccggcttg cggcgcccgc
     3121 ggcggctgtc gctgcgcgaa ggctcgtttg ggtcccaccc atcgcggttg gtttgcatgc
     3181 gggaaatggt ttctcctccg cagtccgcct accggccgct gctgcgcgcg accaagtcgc
     3241 gattctctac gattaccgac gtgactgtga cgaacgacgg cggccccgcg ctgctcaatg
     3301 aactgacgtg gcgtggcctg atcgccgaaa agtcccaagg cctggacgaa cgcctgacca
     3361 gggggccgat cagcggctac atcgggttcg acgcttcggc gcgctcactc cacgtgggcc
     3421 acctgttgca ggttttcatg ctgacccacc tccaacaggc cggcggcacg cccttcgtca
     3481 tcatcggtgg cgggacgggc atgatcggcg acccatcggg caagtcgagc gagcggcagc
     3541 tgctcaacga cgagcagatt cgctccaacg cggcggccat ccaagcgcag ctcgagcgtt
     3601 tcgtcgactt ttcgcccggc cccaaccagg cccagatggt ggacaaccgc gagtggctcg
     3661 ccagttgggg gttgctcgag ttcctgcgcg acgtcgggaa gcacttctcg gtgccgtaca
     3721 tgctcgccaa ggactcggtt caagcgcgct tggctgacgg catgtcattc accgagtttt
     3781 cctacatgac gctccagtcg gccgacttcc tgcagctcta ccggacgcgc ggcgtggaga
     3841 tgcagatggg cggcgccgac cagtggggca acatcaccgc cgggcttgaa ctcattcgac
     3901 gcgtcgttgg tcgcgaggaa ggggaggagt cggccgcgtt cgggctctgc tcgcccctgc
     3961 tgctgacgcg cgacggcaag aagatgggca agtcagagaa gggcgcggtc atgctcgacc
     4021 cctcgatgac gtcgccgtac gacttctacc agtactggct caacgacgat gactccctgg
     4081 cggtccagca cctgcgctgg ttgacgttga tgacggccga
//
LOCUS       DATLCO010000195         4033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1040235, whole genome shotgun sequence.
ACCESSION   DATLCO010000195 DATLCO010000000
VERSION     DATLCO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040235"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VMZ33_04770"
     CDS             <1..1179
                     /locus_tag="VMZ33_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HUR16574.1"
                     /translation="RQDLLAGVDGATANLAADSLAVPTIGDDVMLTIDASLQLRLEKE
                     LYATYVADRAPRVTGLVMDPYTGAILAWASVPGYDANDYPLVAEQSPELFHDPIMQEV
                     YEPGSVMKMLTAAAALERGTVTLDTPVADGKSLHIGENIVRNGDHSSIGVVPFEDIIA
                     RSRNVGTGRVALDLADSTDEGAAILYEMWQRLGIGDATGIELGSEATGIVADPATNPW
                     LVIDLVNRAFGQGVAITPLQLARSFSAMVNGGHLPTPHVISTPGTEIDPGPQVIESAL
                     SETLQQLMVHVVDAGPHYAEETLIQNYVVGGKTGTAQIWDPIAGDWMPDTYNHTFCGF
                     VGAERPEAVILVRIHDTEPRVRKSWGLTLEITSNELFRRVAQDAIDVLEIPPLASSET
                     "
     gene            1207..2592
                     /gene="murF"
                     /locus_tag="VMZ33_04775"
     CDS             1207..2592
                     /gene="murF"
                     /locus_tag="VMZ33_04775"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HUR16575.1"
                     /translation="MADLAGNAGAPVEFDAASLTRALAGRLVRESALPIRGGAVDSRK
                     VQPGNAFFALSGERTDGHLFLADAVQRGATALVVSHEPDDLHGLGDVSVIEVADTLVA
                     LQQAAAEWRTRFSPVVIGITGSLAKTSTKEQIAEVLSERWNVLRNTANENNEIGLPLT
                     MLRLGSEHDVAVLEMGMYQPGDIAVLAALARPSIGVVTAVRSTHLARAGSIDEIERGK
                     RELVEALSPGGTAILNADDPRVARMASHVADGVDVVTYGFDQSADVTAAHVRSLGFEG
                     MEFELRVAGDAVDVTMRALGRHSVHNALAAAAAGVVAGIDLPTIARGLAREWHAPHRS
                     TVIDLGPIKVLDDSYNASPDSMIAALDLLASLPGNRRVAVLGEMLELGDTSESEHRRV
                     EEHARQTADLVLGVGPYSNAVEKAALTSAIDRYVQPGDVVLIKGSRGAAMDDLIPVLE
                     QAALRMAEPVA"
     gene            2589..3581
                     /gene="mraY"
                     /locus_tag="VMZ33_04780"
     CDS             2589..3581
                     /gene="mraY"
                     /locus_tag="VMZ33_04780"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HUR16576.1"
                     /translation="MTIAAPMVQAILLAFALVVILMGPFILLLRRLGFGKRIRIDGPG
                     THYVKEGTPSMGGILMIAVVAGCAVLFHFGTTADFLGPQTIAPLLTLLVIGALGAADD
                     YLNATSGEGIRIRQKLLWQVAAAGFIAYQIQDTYDIRAVSVPFIGDIGVEPWAYILFA
                     AFAIVATSNGVNFTDGLDGLAGGTLIFAFVAYLIIALVAGQINLAILCALMIGALLGF
                     LWFNVHPAQVFMGDAGSLSMGATLAVVGLITGQILLLPLIGIIFVLEVGSDILQIASF
                     KSTGKRIFRMAPLHHHFELAGWDEEKITMRFWIVGVLAALLGVTFFVTTWAPRP"
     gene            3582..>4033
                     /locus_tag="VMZ33_04785"
     CDS             3582..>4033
                     /locus_tag="VMZ33_04785"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16577.1"
                     /translation="MAAPQTQDMIDLRGLTLDSFGGRRVAVLGLARSGVALTRFLHDR
                     GAIVTVYDARSSAELAPQIALLDGRGVRLLVGPDVEPRTALEGQALITTSPSINWRYP
                     TTEQRMRDALRTAEEQGSPVVSEIDLFLRLCPATTVGVTGTKGKTTTSS"
BASE COUNT          661 a         1334 c         1321 g          717 t
ORIGIN      
        1 cgccaggatc tgctcgcggg cgtcgacggc gcgaccgcaa acctggcggc cgacagcctc
       61 gccgtaccga ctatcggcga tgacgtgatg ctgacgatcg acgccagcct gcagctgcga
      121 ctcgaaaagg agctgtacgc aacctatgtc gcggatcgtg cgccacgcgt cacaggattg
      181 gtcatggacc cgtacacggg cgcgattctg gcctgggcat ccgtgccggg atacgacgcc
      241 aacgactatc cgctggtagc ggagcaatca ccagagctgt tccacgaccc gatcatgcag
      301 gaggtctacg agcccggctc ggtgatgaag atgctcacgg ccgcggcagc cctcgagcgc
      361 ggcacggtga cgctcgacac cccggtggct gacggcaagt ccctgcacat cggcgagaac
      421 atcgttcgca acggcgatca cagcagcatc ggcgtcgtgc ccttcgagga catcattgcc
      481 cgatcgcgca acgtgggaac gggtcgcgtt gctctggacc tggctgacag caccgacgag
      541 ggcgcggcca tcctctacga gatgtggcag cggctgggca tcggggacgc gacaggcatc
      601 gagctgggca gcgaggcgac gggcatcgtt gccgaccccg cgaccaaccc gtggctggtg
      661 atagatctcg tgaaccgcgc atttggccag ggagtggcca tcacaccgct gcagcttgca
      721 cggtctttta gtgcaatggt caacggcggt catcttccga caccccatgt gatcagcacg
      781 cccggcaccg agatcgatcc cggcccgcag gtcatcgagt ccgccctgtc cgagacgctc
      841 cagcagctga tggtccacgt ggtcgacgcc ggaccccact atgccgagga aacgctcatc
      901 cagaactacg tggtcggcgg caagactggc accgcgcaga tttgggaccc gatcgccggc
      961 gactggatgc cggacacgta caaccacacg ttctgcgggt ttgtcggcgc cgagcggccc
     1021 gaggcggtca tcctggttcg catccacgac acggagccgc gtgtccggaa gtcgtgggga
     1081 ttgacccttg agatcacctc caacgagctt ttccgacgag tggcgcagga cgccatcgac
     1141 gtgctcgaga tcccgccact ggcgtcttcg gaaacatgaa gcggcccggt gtgaaagtct
     1201 gtggacgtgg ctgatctggc gggtaatgcg ggtgcgcctg tggagttcga tgccgcctcc
     1261 ctcacgcgcg ccttggccgg ccgcctcgtt cgcgaatctg cgctgccaat tcggggcggg
     1321 gccgtcgatt cgcgcaaggt gcagcccggg aacgcgttct ttgcgctgtc gggtgagcgg
     1381 acggatggtc acctattcct ggctgatgct gtccaacggg gagcaacagc tctggtcgta
     1441 tcgcacgagc ccgatgacct gcacgggctg ggtgacgtat ctgtcatcga ggttgctgac
     1501 acgctcgtcg ccctgcagca ggccgccgcc gaatggcgca cccggttctc gcccgtcgtc
     1561 atcggcatca ccggctcgct ggccaagact tccaccaagg agcagatcgc cgaggtcctg
     1621 tctgagcgct ggaatgtgct ccgcaacacg gccaatgaga acaatgagat cgggttgccg
     1681 ctgacgatgt tgcggctggg ttcggagcac gacgtcgcgg tcctcgagat gggtatgtac
     1741 caacctgggg acatcgcggt attggccgcc ctggcgaggc cctcgatcgg cgtcgtgacc
     1801 gccgttcgct caacgcacct ggcacgtgcc ggttccatcg acgaaatcga gcgcggcaag
     1861 cgcgagctgg tcgaagccct gagcccaggc ggaacggcga tcctgaacgc cgacgacccg
     1921 cgcgtcgcgc ggatggcgtc ccacgtcgcc gacggtgtgg acgtggtgac ctacgggttc
     1981 gaccagtccg ctgacgtgac cgccgcgcac gtccgatcgc tgggcttcga ggggatggaa
     2041 ttcgagttac gggttgcggg tgatgccgtc gacgtcacga tgcgggcact tggccggcac
     2101 agcgttcaca acgcgctcgc cgccgcggcc gccggcgtcg tcgccggcat tgaccttccg
     2161 acgatcgccc gcggcctcgc gcgcgaatgg cacgcacccc accgttcaac cgtcattgat
     2221 ctcgggccga tcaaggtgct cgacgactcg tacaacgcgt cgcccgactc catgatcgcg
     2281 gcacttgacc tgctggcgag cctgcccggc aatcgccgcg tggccgtcct cggtgagatg
     2341 ctcgagctgg gcgacacgtc agagtcagaa catcgacgcg tagaagagca tgcccggcag
     2401 accgcggacc tcgtgctcgg ggtcgggcct tactccaatg ccgttgaaaa ggctgctctg
     2461 acctcggcca tcgaccgcta cgtgcaaccg ggagacgtcg tgttgatcaa gggctcgcgc
     2521 ggggcggcaa tggacgacct gatcccggtc cttgagcagg cggcgctgcg catggcggag
     2581 ccggtcgcgt gacgatcgcc gcgccgatgg ttcaagcaat cctcctcgcc ttcgcgctcg
     2641 tcgtcatcct gatgggcccg ttcatcttgc ttctccgacg ccttggcttc gggaagcgca
     2701 tccgtatcga cggacccggc acgcactatg tcaaggaagg caccccatcg atgggcggca
     2761 tcctgatgat tgccgtcgtc gccggatgcg cggtgctctt ccatttcggc acgaccgcgg
     2821 atttcctcgg cccgcagacg atcgcgcccc tgctgaccct gctcgtcatc ggcgcactcg
     2881 gggcggccga cgattacctc aatgccacga gcggggaggg gatccggatc cggcagaagc
     2941 tgttgtggca ggtggccgcg gccggcttta tcgcctacca gattcaggac acgtatgaca
     3001 tccgtgccgt gagcgtgccg ttcatcggcg acatcggcgt tgagccgtgg gcgtacatcc
     3061 tgtttgccgc gttcgcgatc gtggccacga gcaacggcgt taatttcacc gacggcctgg
     3121 acgggctcgc cggcggcacg ctcatcttcg cgttcgttgc ctacctgatc atcgccctcg
     3181 tcgccggcca gatcaacctg gcgatcctgt gcgcgctgat gatcggagcc ctcctgggct
     3241 tcctctggtt caacgtgcac ccggcgcagg tattcatggg cgacgcggga tccttgtcga
     3301 tgggcgcaac cctggccgtc gttggcctca tcacgggcca gatcctgttg ctgcccttga
     3361 tcggcatcat cttcgtgctc gaggtcgggt ccgacatcct gcagatcgcg agcttcaaat
     3421 cgaccggcaa gcgcatcttc cggatggcgc cgctgcacca ccacttcgag cttgccggct
     3481 gggatgaaga gaagatcacg atgcgtttct ggatcgtcgg cgtgctggcg gcactgctgg
     3541 gcgtgacgtt cttcgtcacc acctgggcgc cgaggccctg aatggctgcg ccacagacgc
     3601 aggacatgat cgacctgcgc ggcctcaccc ttgattcgtt cggaggtcgt cgcgtggccg
     3661 tcctcggcct cgcccgcagc ggggtggcgc tgacacggtt cctgcacgac cgcggcgcga
     3721 tcgtgaccgt ttacgacgcg cgctcgtcgg ccgagctggc gccgcagatc gcgctgctcg
     3781 acggtcgtgg cgtgcggctc ctcgtgggcc ccgatgtcga gccgcggacg gcactcgagg
     3841 gtcaggcgct gatcacgact tcgccatcga tcaactggcg atatcccacc acggagcagc
     3901 ggatgcgcga tgcgcttcgc actgccgagg agcaggggag cccggtcgtc agcgagatcg
     3961 acctcttcct gcggttgtgc ccggccacga ccgtcggcgt gaccggcacg aagggcaaga
     4021 ccaccaccag ctc
//
LOCUS       DATLCO010000196         8518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1785409, whole genome shotgun sequence.
ACCESSION   DATLCO010000196 DATLCO010000000
VERSION     DATLCO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785409"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1171)
                     /locus_tag="VMZ33_04790"
     CDS             complement(59..1171)
                     /locus_tag="VMZ33_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16578.1"
                     /translation="MTAGYESYGSASGPTGSRWLWIIGGLIGFVIAAAIGVVALGTFN
                     SGIDPDLGYDLKGANSFALVLALALLVGFVVYAGLEYLQTRRLGSITGQFGTRTLVLM
                     PVAIAFNIILGQAVGAALKIPIYLDSIGTILVGALAGPIAGALTGLLSNLIWSYVLPP
                     PFHSDISGAFAIVAAVIGILAGVFARIGWLRPRPNRNVGQLAVGAIIAVGLIVVLSWL
                     AYLGYTAILDSPDLAPGGDNFVFQLLGWLALALVVGTVIGLILLLFVKRDLSAAYVVV
                     AGVITGLVAASISAPISANVFGGVTGAGTDFIVLAFRQAGADISAAALGQGLISDPID
                     KVVTFFVVYLILGALATRTKARFPQGEKLVAPEPGF"
     gene            complement(1207..2226)
                     /locus_tag="VMZ33_04795"
     CDS             complement(1207..2226)
                     /locus_tag="VMZ33_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HUR16579.1"
                     /translation="MGLTNGHDTRGMPVIIDCDTGIDDALALLYACASPEMDLVGATC
                     VSGNVGAQQVARNTLNVLALGGKSDVEVALGREVPLVRPLVIAPETHGPAGVGYAVLG
                     EPSQPLSERHAADLLIEEARKRPGKIMLITLGPLTNLAVALVREPNLPHLLRGLVMMA
                     GSYRSAGNTAPTTEWNVAVDPEAMQIVLAGWATHSHVPRPVALGLDVTEKAKMTPEHL
                     ADLRRRAGDPPASQLLDFIDDALRFYMEFHSQYDGFYGAFIHDALALAAALDPSLIRT
                     QPVSVEVELGGTHTTGETVTDWRHRWGREPNLDVAVEADIGKFFDRFVERVGMLAARQ
                     SDVAL"
     gene            2306..3028
                     /locus_tag="VMZ33_04800"
     CDS             2306..3028
                     /locus_tag="VMZ33_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylmercury lyase family protein"
                     /protein_id="HUR16580.1"
                     /translation="MGDSVGQSSAEQITEASLAQRVRLHVYEHFVEKARPPVVEELMS
                     HFDLSRAEVVEVLKDLEQSRHLALVRGTARILMAFPLSAIATPFRVAADGRDYFANCA
                     WDAVAFHTMLGTDTDIRIDSYCHHCGSPIVVEMSSGHAVTVDPANTIVYLALRPTQWW
                     EDIITTCSNTMVFFCSQEHRDASNLAGSPADGASLTPQQVHELGVPLYSKKLAIDYVR
                     PGRDELMAHFAALGLTSPYWQI"
     gene            3031..3519
                     /gene="purE"
                     /locus_tag="VMZ33_04805"
     CDS             3031..3519
                     /gene="purE"
                     /locus_tag="VMZ33_04805"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HUR16581.1"
                     /translation="MADSRFVAIIVGSRSDLEIVQKAADTLAGLGIESELRVISAHRA
                     PDLLESYVDDAKARGAAVFICAAGLAAHLPGVVASKTTGPVIGLPMPGNVAGGLDALL
                     SIVQMPKGVPVATVGIGQAENAALLAAQMLGITDVTVAQAVADHRAAARRQIEEDPTN
                     VA"
     gene            3595..3996
                     /locus_tag="VMZ33_04810"
     CDS             3595..3996
                     /locus_tag="VMZ33_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16582.1"
                     /translation="MDSDPMPVMDRQPVPSRRDGRSEPPQRVPETSPPLLGDWFIYLS
                     VIILVCGVIVITALNFGAGPANGIVRFPALIGAGILVIVSADAAVRTYRSIGAWWNVD
                     RPRAVFRVVWSVVLAGILVASVALIYVLLTT"
     gene            4004..5401
                     /locus_tag="VMZ33_04815"
     CDS             4004..5401
                     /locus_tag="VMZ33_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HUR16583.1"
                     /translation="MIVRGVWVHLLLGAAMAVVVIGINAPEGGLDPFPEYAERIVTGG
                     IPYRDYPFEYPPLALFPLAMPRLLAGANDYIPAFAVLSFVFAVATTIAVGLVARRGWS
                     GAGNGTTAETGLVFGGLALAALPLILFRFDIFAAMFAALALLAVAFRRPGWVGAALGF
                     GALVKIFPAFLLPVVFLYYVFNRERTAAVHLVAGFVVSTAAVLAEVAIYAGPRQTLAW
                     LTYQDRRGVEIESIHGSLAMLGDVLGGPASTITFGFGSYEVTSALLDPLGVPLLLLGV
                     TLVVGVIAGALASFRHDVRRLGRVQVSTLVTYLTATVLLIIVTNKVLSPQYMVWLFPF
                     APLLRLRHSLTLLVAQLLTTIEYPLAFADLRALDPKLVWLVNLRNAILIGLLIWLIVP
                     GLALLRPRRGQTQGQSVAMLEMPPRSPAETPNMSNPMASRLRCGAIRVRSSAAKVTVA
                     TLARVRPGRPSSLAR"
     gene            complement(5701..6669)
                     /locus_tag="VMZ33_04820"
     CDS             complement(5701..6669)
                     /locus_tag="VMZ33_04820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR16584.1"
                     /translation="MSIARRYLTGYPMRVLVWRDGAGALAGVAALSVIGDRPPIRPIR
                     QLALWGTIGPRMRGLTDVLARDDVRTEVLDGFAAWLRGNKEWDALRVVRPQHGSPTPG
                     RLRQEAQATGWTYRPYTNVRSTTFQLDLPADEEGWAKHLGSKARKVMRWEMRKFADFR
                     TGVVKPAVEGAELTEALESIERLLHERWGDNEVYFVNDSEFRGLLHETVPALVEHRGA
                     WVSTARDQSGVRAVLITFAQNGYAMAFMIAMTTDPDYSKFSLGKHIFDAGLAEAVRRG
                     CHNYDFLWVGGYKESFWHATPRELESAIVGRGLLGRLATRLLAGRF"
     gene            complement(6792..8282)
                     /locus_tag="VMZ33_04825"
     CDS             complement(6792..8282)
                     /locus_tag="VMZ33_04825"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUR16585.1"
                     /translation="MTVDTAATNENATASPPRDAKMFINGEQVDALDGQTFEVHNPAT
                     GAVIARVPLAGKADVDRAVESAQGAFEGPFAKWSAAKRGRTLAKFSDLVKRNVEELAQ
                     IDRENVGKPISASRNESFAVSLVLEYYAGAANKHFGETIPTSMPGFDFTMREPIGVVA
                     AIVPWNFPQNMAAWKLGPALATGNTVIIKPASWTPLSAIRLAQLALEAGFPPGVVNVV
                     TGPGGTAGAALAGHPGVGKVAFTGETTTGQEVMRLAANNVKKISLELGGKSPSVVFAD
                     ADLERFAAAAPMAVFDNAGQNCTARSRILVERSVHERVVELFAEHTKKLVVGDPADDK
                     TQVGSLVSPRQLERVQEFVDSARDEGATLVAGGVAPDDPGLANGAFLMPTIFDGASAD
                     MRFIREEIFGPVVGIIPFDTEEEAVRIANATPYGLAGSIWTRDIGRALRVAKGIRAGT
                     LSINSNDSIHTEAPFGGYKMSGIGRELGMHALDLYTEVKNVYVDLS"
     gene            complement(8301..>8518)
                     /locus_tag="VMZ33_04830"
     CDS             complement(8301..>8518)
                     /locus_tag="VMZ33_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16586.1"
                     /translation="DHPRTSHVLTDRPDHWLEVGVDFQTGVMLLLAEHIGDRLTRHAQ
                     ATAIALDEPITDDVFRLHISSDARTIY"
BASE COUNT         1385 a         2822 c         2813 g         1498 t
ORIGIN      
        1 agtgggttga gccggtggta gggactcggg ccggggcgac ccaggaactc cgggacggtc
       61 agaagccggg ctctggcgcg acgagcttct cgccctgcgg gaagcgcgcc ttggtgcgcg
      121 tggccagcgc gccgaggatc aggtagacca caaagaaggt cacgaccttg tcgatcgggt
      181 cggagatcaa cccctggccg agcgcggcgg ccgagatgtc ggcaccggct tggcggaagg
      241 cgaggacgat gaaatctgtc cctgcgccgg taaccccgcc gaagacgttg gcggaaatgg
      301 gtgccgagat tgacgcggcg accagtccgg tgatcacgcc cgcgaccacg acataagccg
      361 cgctgagatc gcgcttcacg aagagcagaa ggatcagccc gataacggtc ccgacaacga
      421 gcgcgagcgc cagccagccg agcagctgga atacgaagtt atctccgccc ggcgccagat
      481 cagggctgtc gaggatcgcc gtgtagccga ggtaggcgag ccaggagaga acgacgatca
      541 ggccgacagc gatgatggcg ccgaccgcca gctggcccac gttgcgattg gggcggggac
      601 gtagccaacc gatgcgcgcg aagacgccgg ccaggatgcc gatcacggcc gcgacgatcg
      661 cgaaggcgcc gctgatgtcg ctgtggaacg gcggcggaag gacgtagctc cagatcaggt
      721 tggaaagcag ccccgtcaag gccccggcga tagggccggc caacgcaccg acgaggatcg
      781 tgccgatcga gtcgagatag atggggatct tcagcgccgc gccaacagcc tggccgagga
      841 tgatgttgaa cgcgatcgcc accggcatga gcaccagggt gcgggtgccg aactggcccg
      901 taatcgaccc caggcgccgc gtctgcaggt attccagccc cgcgtagacg acgaagccga
      961 ccaacagcgc cagggccagg accagcgcga acgagttggc gcctttgagg tcgtacccga
     1021 ggtcggggtc gatccccgag ttgaacgtac cgagtgcgac aacgccgatt gcggcggcga
     1081 taacgaaccc gatgagtccg ccgatgatcc acagccaacg tgaaccggtc ggtccggatg
     1141 cgctgccgta agactcgtaa cccgctgtca ctcccagccc tcctcggctc tccctgattc
     1201 cctgtgtcag agtgccacgt cggactgcct cgcggccagc attccgacac gttcgacgaa
     1261 ccgatcgaag aatttcccaa tatcggcctc gaccgcgacg tcgagatttg gctcgcgccc
     1321 ccagcggtgg cgccagtcgg tcacagtctc gccggtcgtg tgcgtgccgc cgagctcgac
     1381 ctcaacgctc accggctgag tcctgattag tgacgggtcc agggcggcgg ccagtgcgag
     1441 cgcatcgtgg atgaaggcgc cgtagaaacc gtcgtactgg ctgtggaatt ccatgtagaa
     1501 acgcagcgcg tcgtcgatga agtcgagcag ctgcgacgca ggcgggtcgc cggctcgtcg
     1561 tcgcaggtcg gccagatgtt cgggcgtcat cttggccttt tcggtcacgt ccaatcccag
     1621 cgccacgggc cggggaacgt gtgaatgcgt ggcccagccg gcgaggacga tctgcatggc
     1681 ctcggggtcg acggcgacgt tccactcggt tgtcggcgcc gtattgccgg ccgaccggta
     1741 ggaaccggcc atcatcacca ggccgcgcaa caagtgcggc aggttcggct cgcgaacgag
     1801 agccacggcc agattggtga gtggcccgag ggtgatcagc atgatcttac ccggccgctt
     1861 gcgggcctcc tcgatgagca gatcggcggc gtggcgctcg ctcagcggct ggcttggctc
     1921 gccgagcacc gcgtacccga cgccggcggg accatgcgtc tccggcgcga tcaccaacgg
     1981 gcgcaccagc ggcacctccc gtccaagcgc gacctctaca tcgctcttac cgccaagtgc
     2041 caggacgttg agcgtgttgc gagcgacttg ctgcgccccg acgttgccgg acacgcacgt
     2101 ggccccgacc aggtccatct ccggtgaggc gcaggcgtag agcagtgcca gtgcgtcatc
     2161 gatacccgta tcgcaatcga tgatgaccgg catcccgcgc gtgtcatgac cgttagtcag
     2221 gcccatggtg cgaaatgatc ggcgacctgg gccacgggcg caccttccac ggacagtttg
     2281 tgcgggagcg ccgaaactat ggccaatggg tgattcagtg ggtcagtcat cggccgagca
     2341 gattacagag gcgagcctcg cgcagcgcgt gcggctgcat gtctacgagc acttcgtcga
     2401 gaaggcgcgg ccgcccgttg tggaagagct gatgtcgcac ttcgacctat cgcgagccga
     2461 ggtggtcgag gtgctcaagg acctcgagca gtcgcgccac ctcgcgctcg tgaggggcac
     2521 cgcgcggata ctcatggcat tcccgctctc ggcgatcgcc acgcccttcc gggtcgctgc
     2581 cgatggccgc gactacttcg cgaactgcgc ctgggacgcc gtggcgttcc atacgatgct
     2641 cggcacggat acggatatcc gaatcgactc gtactgtcac cactgcggtt caccgatcgt
     2701 cgtcgagatg agcagcggtc acgccgtgac cgtggacccc gcgaacacca tcgtctacct
     2761 tgcgctacgg ccgacacagt ggtgggagga catcatcacg acctgctcaa acaccatggt
     2821 cttcttctgc tcccaagagc atcgtgatgc ctccaacctt gcgggctcgc ccgcggacgg
     2881 cgcctcgctc acgccccagc aggttcatga gctgggcgta ccgctctact cgaagaagct
     2941 ggccatcgac tacgttcgac ccggccgcga tgagctgatg gcgcacttcg ccgctctcgg
     3001 cctcaccagc ccgtattggc agatctgacg atggccgact cccgcttcgt tgcgatcatc
     3061 gttggcagcc gatccgatct tgagatcgtc cagaaggcgg ccgacacgct ggccggcctg
     3121 ggtatcgaaa gcgagctgcg ggtcatctcg gcccaccggg ccccggatct gctggagtcg
     3181 tacgtggacg acgccaaggc acgcggcgcc gcggttttca tctgtgccgc cggattggcg
     3241 gcgcacctgc cgggagtcgt ggcgtccaag acgaccggtc cggtgatcgg cttgcccatg
     3301 ccgggcaacg tcgctggtgg cctggacgca ctgctgtcca ttgtccagat gcccaaaggc
     3361 gtgccggtcg ccaccgtcgg catcgggcag gccgagaatg ccgcgctgct tgccgcgcag
     3421 atgctgggga tcacagacgt aacggtggcg caggccgtgg cggaccaccg cgccgccgcg
     3481 cgacggcaga tcgaggaaga cccgacgaac gtcgcttgac tcagcgggca cttgggatgc
     3541 cgcgcagccg acgctaaacg gggccacaaa cgcgtcgctc cctagactcc ggacgtggac
     3601 agcgacccca tgccggttat ggaccgtcag ccggttccat cgcgccgcga cggccgttcc
     3661 gagccgccgc agcgcgtccc agagactagc ccaccgctgc ttggcgactg gttcatctat
     3721 ctctcggtaa tcatcctcgt ctgtggcgtg atcgtgatca ctgctctcaa cttcggcgcc
     3781 ggtccggcta atgggatcgt ccggtttccg gcgctgatcg gagccgggat cctcgtaatc
     3841 gtctccgctg acgccgctgt gcggacctat cgctcgatcg gcgcttggtg gaacgtcgac
     3901 cgcccgcgcg ccgtctttcg tgtcgtgtgg tccgtagtgc tcgccggcat ccttgtcgcc
     3961 agcgtcgcgc tcatctacgt cctgctgacc acgtgacgtt cagttgatcg tccgcggcgt
     4021 atgggttcac ctcctgctgg gcgccgccat ggcggtcgtc gtgatcggga tcaatgcgcc
     4081 tgagggcgga ttggacccct tcccggagta cgccgaacga atagtcacag gcggcattcc
     4141 gtaccgcgac tacccgttcg aatatccgcc gctggccctt ttcccgctgg ccatgccgag
     4201 actgctcgcc ggtgcgaacg actacatccc cgcgttcgct gtcctgtcat ttgtcttcgc
     4261 cgtcgcaaca accattgctg tcggcctcgt agcgcgacgc ggctggtcgg gcgccggaaa
     4321 cggaacgacc gcggaaacgg ggctcgtctt cggcggactt gcgctggctg cactgccgct
     4381 catcctgttc aggttcgaca tcttcgcggc gatgttcgcg gcgctcgcgc tgctggcggt
     4441 ggcattccgc cgccctggct gggtcggcgc cgcactcggg ttcggggcgc tggtcaagat
     4501 cttcccggca ttcctgctac ccgtcgtatt cctgtactac gtgttcaacc gcgaacggac
     4561 cgctgccgtt cacctggtcg ctggcttcgt cgtgtcgacc gccgccgtcc tcgcggaggt
     4621 ggcgatttac gccggtccgc ggcagacgct ggcatggctg acgtaccagg atcgccgcgg
     4681 cgtcgagatc gagagcatcc acggcagtct ggcgatgctg ggcgacgtgc tcggggggcc
     4741 cgcgtccacg atcacgtttg gcttcggctc gtacgaggtc acatctgctc tgctggaccc
     4801 tttgggcgtc ccgctgctcc tgctcggagt aacgctggtc gtcggggtaa ttgctggggc
     4861 cctcgccagc ttccgccacg atgtccgacg actcggtcgt gtgcaggtct caacgctggt
     4921 cacgtacctc accgccacag tgctcctgat catcgtcacg aacaaggtcc tgtcgccgca
     4981 atacatggtg tggcttttcc cattcgcgcc acttctgcgg ctgcgccact cgctgacgct
     5041 gctagttgcc cagctcttga cgaccatcga atacccgctc gcattcgctg acctgcgcgc
     5101 gctcgatccc aagctcgttt ggctcgtcaa tttgcgcaac gcgatcctga tcgggctgtt
     5161 gatctggctg atcgtgcccg gcctggcgct tctgcgacca cggcgcggtc agacccaagg
     5221 tcagagcgtg gcgatgttgg agatgcctcc gaggagcccc gccgagacgc cgaatatgag
     5281 caatcccatg gccagccgtt tgcgctgcgg cgcgatcagg gtcaggagca gcgctgcgaa
     5341 ggttacggtc gcaaccttgg ccagggtcag gccgggcagg ccgagctcgt tggctagatg
     5401 aaccacgacg gggtttgctt cggcgcccag tccgtgacgg tcgatcatca ccaggaatga
     5461 cgcgtagtcg aataggtgcg ccattgccag cgcggcgagt gagaacgcaa gtggcacggc
     5521 ccgagtctgc tccacacaga acgggatcgg gctcgccact gctccgcgcg gcctatcggc
     5581 gaccgttcag ttcgccgcgc atcaaagctt cccgtgattg gctcaattct ttccgattgg
     5641 cccgccgctg tcgatacccg tgacccggcc tcggttgagg cgcggcaccg ccgccgtcgg
     5701 ctagaagcgg cccgccagca gcctcgtcgc cagccggcca aggaggcccc gcccgacgat
     5761 cgcactctcc agttcacgtg gcgtcgcgtg ccagaagctc tccttgtagc cacccaccca
     5821 caggaaatcg tagttgtggc acccgcggcg gacagcctcg gccagaccgg cgtcgaagat
     5881 gtgcttgccg agtgagaatt tggagtagtc gggatcggtc gtcatcgcga tcatgaacgc
     5941 catcgcgtag ccgttctggg cgaaggtgat gaggacggcg cggacgcccg attggtcgcg
     6001 cgcggtggag acccacgcgc cgcggtgctc cacgagcgcg ggcacggttt cgtggaggag
     6061 tccgcggaac tctgagtcat tgacgaagta cacctcgttg tcgccccagc gctcgtgcag
     6121 cagccgctcg atggactcca gcgcctcggt cagttccgca ccctcgactg ccggcttcac
     6181 gaccccagtg cggaagtccg cgaacttgcg catctcccag cgcatgacct tgcgcgcctt
     6241 ggagccgagg tgctttgccc acccctcctc gtcagccggc aggtcgagct ggaacgtcgt
     6301 cgaacggacg ttggtgtagg gccgataagt ccaaccggtg gcctgcgcct cctgacgcaa
     6361 ccggccgggc gttggcgagc cgtgctgagg gcggacgacg cgcagcgcgt cccactcctt
     6421 gttgccgcgg agccatgcgg cgaacccgtc gaggacctcg gtgcggacgt cgtcgcgtgc
     6481 caggacatcg gtcaggccac gcattcgggg gccgatcgtg ccccacaggg caagctggcg
     6541 gatcgggcgg atcggtggtc gatcgccgat gacgctgagc gcggcgacgc ccgccagcgc
     6601 cccggcgccg tcacgccaaa cgagtacgcg catcgggtag ccggtgagat aacggcgtgc
     6661 gatcgacacg agccaatccg gcgcatccag cgcagcgcgt gcagacgtcg cagccagcgc
     6721 ccgccactcg ggcagtagct gttcgaccgc cgctagctcg gtcagaacct ccggcggact
     6781 gagcgtggcg ctcagctcag gtcgacgtag acattcttga cctctgtgta gagatcaagc
     6841 gcatgcatgc ccagctcgcg tccgatgccc gacatcttgt agccaccgaa cggtgcctcg
     6901 gtgtggatcg agtcgttgct gttgatcgaa agcgtgcccg ctcggatgcc cttcgccacg
     6961 cgcagggcgc ggccgatgtc gcgcgtccag atcgagcccg ccaggccgta cggcgtcgcg
     7021 ttggcgatgc ggaccgcctc ctcctccgtg tcgaagggga tgatgccgac gaccgggccg
     7081 aagatctcct cgcggatgaa gcgcatgtcg gcgctcgcgc cgtcgaagat cgtgggcatc
     7141 aagaatgcgc cgttggcgag gccggggtcg tcaggcgcca cgccaccggc gacgagcgtc
     7201 gcgccctcgt cgcgtgccga gtcgacgaac tcctggacgc gttcgagctg gcgtgggctt
     7261 acgaggctgc cgacctgcgt cttgtcgtcg gctgggtcgc cgacgaccag cttcttcgtg
     7321 tgctcggcga acagctcgac cacccgttcg tggacggagc gctcgacgag gatccggctg
     7381 cgggcagtgc agttctggcc ggcattgtcg aagacggcca tcggtgccgc ggcggcgaac
     7441 ctctccaggt cggcgtcggc gaacaccacg ctgggcgact tcccgccgag ctccagggag
     7501 atcttcttaa cgttgttggc ggccagccgc atgacctcct ggcctgtcgt cgtctcaccc
     7561 gtgaacgcga ccttgcctac tcccggatgc ccggcgagcg ctgctccggc cgttccgccc
     7621 gggcccgtga cgacgttgac gacgccgggt gggaagccag cctccaacgc gagctgtgcg
     7681 aggcggatcg ccgacagcgg cgtccaggat gcgggcttga tgatcacggt gttgccggtt
     7741 gccagcgccg ggccgagctt ccacgccgcc atgttctggg ggaagttcca gggcacgatc
     7801 gccgccacca cgccgatcgg ctcgcgcatg gtgaaatcga agccgggcat ggacgtgggg
     7861 atcgtctcgc cgaagtgctt gttcgccgcg ccggcgtagt actcgagcac gaggctgacc
     7921 gcgaatgatt catttcgcga tgcgctgatc ggcttgccga cgttctcgcg gtcgatctgc
     7981 gccagctcct cgacgttgcg cttcacgagg tcggagaact tcgccagggt tcggccgcgc
     8041 ttggccgccg accacttggc aaacggccct tcaaaggctc cctgggccga ctctacggcg
     8101 cggtcaacgt ctgccttgcc cgccagcgga acgcgcgcga tgaccgcgcc ggtcgcgggg
     8161 ttgtgcacct caaacgtctg tccgtcgagt gcgtcgacct gttcgccgtt gatgaacatc
     8221 tttgcatcgc gcggcgggct cgccgtcgca ttctcgttcg tcgcggccgt gtcaacggtc
     8281 attgtcaaat tcctccccgc ctagtagatc gtgcgggcat cgcttgaaat gtggagtcgg
     8341 aagacgtcgt cggtaatcgg ttcgtcgagc gcgatggcgg tggcctgcgc atggcgtgtg
     8401 aggcgatcgc cgatgtgctc ggccagcagc agcatcactc ccgtctggaa gtcgacgccg
     8461 acctcaagcc agtgatccgg acgatcagtc aggacatggc tcgtgcgcgg atggtcga
//
LOCUS       DATLCO010000197         3360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7598863, whole genome shotgun sequence.
ACCESSION   DATLCO010000197 DATLCO010000000
VERSION     DATLCO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7598863"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VMZ33_04835"
     CDS             <1..687
                     /locus_tag="VMZ33_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16587.1"
                     /translation="LVRAGVRTLLESGGGVEVVAEADTVDEALQTARDTPLDVVLIDI
                     DEATTDAVDDMRRLRSELSDDAAVVVMARNDDDEGVYRAVVGGAVGHVGENAAPAELV
                     DTIREAASGAEPISKTLAERPAVARRVLETYADMVARGPVATQPQVTDRELMILGHAA
                     NGMTNQQIGRALGVSEHTVKSSISQILTRLGLRHRTGAVVHALRHGWITVAQTNKTAA
                     GKADGRSSEF"
     gene            complement(716..994)
                     /locus_tag="VMZ33_04840"
     CDS             complement(716..994)
                     /locus_tag="VMZ33_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HUR16588.1"
                     /translation="MTNIIAWIVLGAIAGLIAGMLVKGDERWGIIGHIVLGIVGALVG
                     GFIANALGIGSGREGGDVLNIMSVIVAVVGAVIVVLVVNMLTRNRRTV"
     gene            complement(1061..1558)
                     /locus_tag="VMZ33_04845"
     CDS             complement(1061..1558)
                     /locus_tag="VMZ33_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HUR16589.1"
                     /translation="MSDEYRRLLAESERYAEQVDRTKLAAPPLAGIAIVTCMDSRIVP
                     DAIFGLRPGDANVIRNAGAVATEDVLRSLVLSRNVLGTTEIVVMGHTGCGLYHLDEAA
                     LRARLASQTGRESKTTFGSFADVDEHVRRQVATIRAHPWISPIEIHGMIYEVETGRVR
                     DVARV"
     gene            complement(1551..>3360)
                     /locus_tag="VMZ33_04850"
     CDS             complement(1551..>3360)
                     /locus_tag="VMZ33_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HUR16590.1"
                     /translation="VAAATAQAADKGIRVVVGGGTFVLPLIHKVGKLKLTARQISVAL
                     PDAVTSQGIKVAVQGVATFKIGRDVESIRNAAERFLDSRDEQVDSIVKNVLEGSLRSI
                     VGTLTIEELIMDRQKLLQQVQDSAKGDLATSGLQIDAFTIQSINDESGYVDLLGQQKL
                     AIVERDARMAKATADQEAAVREAEANQVKISAERDVELRRADVLTQTAAAQARADQAG
                     PLASAEAQQEVTKRQTELADLEAARKQKELLTSTVRPAEAEAEAVVRRAEGDKQARIA
                     NAQAAAEQEKLAGQAQAAVIQFKGEANAHVIEVNAKAEAQRVTLEGNAEAGITFTKGE
                     AEAKALALRAEAFQKFNEAAIIQTVLGMLPEIVRAAAEPMSSIDNLTVLSNDGASELV
                     KNTTRTVAEASATVKGLTGLDVPALISNAMGGATRGGGRGSEGSAGSGGPGSGGGGSQ
                     RSTKTPAPTVPMPPAAASGATGTSGTTPKPADTMDQASAAISRASEAITRATRQVTAP
                     AATLGAAPTRAPIMSEMSVSDAAAVLADQLRQIPGIERHVTIPLAELDQRGPRPVRAA
                     WRLARDRVAGKYGSMTVGQLLTEFEKHGQEPPSLSV"
BASE COUNT          538 a         1120 c         1094 g          608 t
ORIGIN      
        1 ctggtacgcg ccggcgttcg caccttgcta gagagcgggg gcggcgtcga agtcgtcgcc
       61 gaagcggaca ccgtcgatga ggctcttcag actgcgcgcg ataccccgct ggacgttgtg
      121 ctgatcgaca tcgacgaggc aacgacggac gccgtcgacg atatgcgacg gctccgaagt
      181 gagctatcgg acgacgccgc agtggtcgtc atggcccgaa acgacgatga cgagggggtt
      241 tatcgggcgg tagtcggcgg tgccgtgggc catgtcgggg aaaacgctgc cccagcggag
      301 ttggtcgaca ccatcaggga agcggcatcg ggggccgagc ccatctccaa gacactggcc
      361 gagcggccgg cagtggcgcg acgcgtcctc gagacctacg ccgacatggt cgctcgcggg
      421 ccggtagcga ctcagcccca agtgaccgat cgcgagctga tgatcctggg ccacgccgcc
      481 aacggaatga ccaaccagca aatcgggcga gccctcggtg tcagcgagca cacggtcaag
      541 agctcgatct cgcagatcct cacacggctc ggacttcggc accgaactgg agcagtcgtc
      601 catgcgttgc gccacggctg gatcactgtc gcccagacaa acaaaacggc cgccggcaaa
      661 gccgacggcc gttcctcgga gttctgatct gacctgagtc gtcagatcgc ggggttcata
      721 ccgtgcggcg gttccgcgtc agcatgttca ctaccagcac gacgatcacg gcgccgacga
      781 cggcgacgat gaccgacatg atgttgagga cgtcgccgcc ctcacggccg ctaccgatgc
      841 cgagcgcgtt ggcgatgaac ccaccgacga gggcaccgac gataccgagc acgatgtgcc
      901 caatgatgcc ccagcgctca tcacccttga cgagcatgcc tgcaatcagg ccggcgatcg
      961 cgccgagcac gatccacgcg atgatgttgg tcattcagct acctctcctc tattcctcgt
     1021 ttaccgggcc ccctaagaga atagtccacg agcagggggt tcaaacgcgc gctacgtcgc
     1081 gcacccttcc tgtttcgacc tcgtaaatca ttccgtgaat ttctatcgga cttatccatg
     1141 gatgggcccg gatcgttgca acttggcgac gaacgtgttc gtcgacgtcg gcgaaagacc
     1201 cgaatgtggt tttggactcg cgacctgttt gagacgcgag tcgggcacgt aaagcggcct
     1261 cgtcgaggtg gtacaagcca catccggtgt ggcccataac cacgatctcg gttgtcccga
     1321 ggacgttgcg gctgagcacc agagagcgca gcacgtcctc cgtcgcgacg gcgccagcgt
     1381 tgcggatcac gttggcgtca ccgggtcgta gcccgaatat ggcgtcgggc acgatccgcg
     1441 agtccatgca cgtgactatg gcgatgcccg ccagcggcgg agccgcaagc ttggtccggt
     1501 caacctgttc ggcgtagcgc tcgctctcgg cgagcagccg gcgatactcg tcagacagag
     1561 agcgacggcg gctcctggcc gtgcttctcg aattccgtga gcagctggcc gaccgtcatc
     1621 gagccgtatt tgccagcaac gcggtcgcga gcgaggcgcc atgcggcgcg aaccggtcgc
     1681 gggccgcgct ggtcgagctc tgccaacggg atcgttacgt gccgctcgat acccgggatc
     1741 tgccggagct gatcggccag gacggccgcc gcgtcggaca cggacatctc tgacatgatc
     1801 ggcgcgcggg tcggcgcggc ccccagcgtg gcggccggtg ccgtcacctg ccgcgtggcg
     1861 cgagtgatcg cctcgctggc acgtgagatg gccgcagatg cctgatccat ggtgtccgca
     1921 ggcttcggtg tcgtgccgga cgtgccggtt gcacctgacg ccgcggccgg gggcatgggc
     1981 acggtcggtg ccggggtctt ggtcgagcgc tggctccctc ccccgcctga gcctggtccg
     2041 ccggagccag ccgagccttc ggagcctcgc ccgccgcccc tggtggcgcc gcccatagcg
     2101 ttcgagatca gcgccggcac gtccaggccg gttagccctt tcaccgtggc gcttgcctcg
     2161 gccactgtcc gcgtcgtgtt cttgacgagc tccgatgcgc cgtcattcga gaggaccgtc
     2221 aggttgtcga tcgaagacat cggctcggcc gcggcgcgga cgatctcggg caacatcccc
     2281 agaacggtct ggatgatggc tgcctcattg aacttctgga aagcctcggc gcggagggcc
     2341 agcgcctttg cttcggcctc gcctttggtg aatgtgatgc cagcctcggc gttgccttcg
     2401 agcgtcacgc gctgggcctc agccttggca ttgacctcga tgacgtgggc gttcgcctcg
     2461 cccttgaact ggatgacggc cgcctgcgcc tgaccggcga gcttctcctg ctccgccgcc
     2521 gcctgggcgt tagcgattcg cgcctgcttg tcgccctcgg ctcgccgcac cacagcttca
     2581 gcctcggctt cggccggccg gacggtcgag gtcagcagtt ccttctgctt gcgcgccgcc
     2641 tcgaggtcgg ccagctcggt ctgccgcttg gtgacctctt gctgtgcttc ggcagaggca
     2701 agcggacccg cctgatcggc ccgtgcctgg gcggcggccg tttgggtaag cacgtcagcg
     2761 cggcgcagct cgacgtcacg ctcggcactg atcttgacct ggttcgcctc ggcctcgcga
     2821 accgcggcct cctggtcggc agtcgccttc gccatgcgcg cgtcgcgctc gacaatcgca
     2881 agcttctgtt ggccgagcag atcgacgtag cccgactcgt cgttgatcga ctggatcgtg
     2941 aacgcgtcga tttgcagccc cgacgtggcc aggtcgccct tggccgaatc ctggacctgc
     3001 tgtaggagct tctggcgatc catgatcaac tcctcgatcg tgagcgtgcc cacgatcgac
     3061 ctgagcgacc cctcgaggac gttcttgacg atggagtcga cctgctcatc gcgcgagtcg
     3121 aggaagcgtt ccgcggcatt acggatggac tcgacgtcac ggccgatctt gaacgtggcc
     3181 acgccctgca cggcgacctt gatgccctgg cttgtcactg cgtccggcag tgcgacgctg
     3241 atctgccgcg ctgtcagctt gagcttgccg accttgtgga tcagtggcag aacaaaagtg
     3301 ccgcctccga ccacaacacg gatccccttg tcagcggcct gcgcggtggc ggctgcgacc
//
LOCUS       DATLCO010000198         5415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6857624, whole genome shotgun sequence.
ACCESSION   DATLCO010000198 DATLCO010000000
VERSION     DATLCO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6857624"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..786)
                     /locus_tag="VMZ33_04855"
     CDS             complement(94..786)
                     /locus_tag="VMZ33_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16591.1"
                     /translation="MTNISGMAVRHVASVVLCALAAACSSAVPGAVAPSATTQPPAAA
                     TPTTAVAPPLRPASPTPTLTPVTIEAPSLSAPPEPTTPADYLISLAHAGLRSTCFIRD
                     EIYDGEIASVTCGPDDLTFDYSLFVTPDEMNAAFDEDVAGAETPPDPKSTDCASGRHL
                     TDYSFDEGESLGRLNCREHLSSSGALYHVLEWTSHEFLVIGYLSNRAEAHTWDELVEF
                     WHERAGPIGLRQ"
     gene            complement(848..4666)
                     /locus_tag="VMZ33_04860"
     CDS             complement(848..4666)
                     /locus_tag="VMZ33_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HUR16592.1"
                     /translation="MLLLLALAAPASTLAVTPGLGGLSDKDFRAGSVQPTSAQISRVT
                     QLGASVRWNRWGTPQSLIKRSGWLATGLSGTPETAARNWIRTNRTLFRLTDTDVTNLE
                     LVSSNPIGQGRAILFRQRYGTLKAAQDGLITVAIIGGKVAYASSSATGSKSAPQSAIL
                     SAQQAWLKAAAHVGKPTVGGALGAVRTVGDWSVFSATALRTPTYNDKSSGAVDQRARL
                     VAVPTPNSDRAVVAFETIVLDTSGAEPLAYTVFVDARSGSILMRYNRVDHLDQQDDFQ
                     VSTGTNDEPTFGQFSGTTDANGQCGPDHAIVAPTGTTSITVAASAVIPANDIVLYLYN
                     SALVEVANMDSGTSPEAIHYEIAPPLPAPATYYARVCQFAADDPLEPFNYEGAFATND
                     ADTSAAQFNYPPKWKHFRAHPSLANDPSPEDYDLANTDTRVIGCWVLVHNGGNVPGCT
                     TSVGKLQNLASRVPWDHIVQTNAPSFTTIGNNAISGEAWTTPLTPGPLQQRPVSLDRD
                     YSFPWTNAWNQSRCATTNLVPGGNDIDAAVTNLFVAHNRMHDWSYFLGFTEDAYNLQT
                     NNFGNNPGPLAENDPEFGQAQSGALLPVEAGVSRDNANQTTLNDGVPGITNMYLWQPI
                     AGGFYSPCVDGDYDMAVIGHEYGHAISNRMAGGPDSNLSGTQARAMGESWSDLMAMEY
                     LNEFGLVPTNGESRYAVGAYATGNNQRGIRNFNMSTSPLNYSDVGYDFVCNQTILGPD
                     IEGTCPDGRTQVHADGEIWSATNFDLRQAFIAKYNGSYPASNDSRQRACAQGTYPASS
                     CPGNRRWAQIMFDAWLLMPAAVSMLDARDAYLAADMIRFNGANQKLLWNVFAHRGMGM
                     NASSAGASDVDPKPDFQSPLEQFKSVTFKVVAADEGNALITNAKVYVGQFEAAANPIA
                     DTIPAGALTNTAKFVNGEYEFFVAAPGYGHVRFPRNIVGTGNVTVEIRMSTNRASLTK
                     GAVATGTGTNFNNLFDDTELTNWTGPSPVVAGAALATVDLQGGVQNIKRVNVSAMLNP
                     ENGGRFRALRQFEIWTCNGSAATCAVNTNFTKIYTSPANAFPGVRPRPTVPDLILRTF
                     DVPDTNATHVQLRVVSNQCTATGTGFRGDQDADPLNDSDCVTGSDADLDVKAAELQVF
                     SSTPSLPARDPAVAVTLAAPATAASGSQITYTATYTNVGPNESSSAVLTDVLPSGLTF
                     VSASNSGTYNASTRTVRWSLGTVNVGYTGTRTVKVAVNGAVGDAILNQVTYTAPETVA
                     VSIPVVTAIE"
     gene            4991..>5415
                     /locus_tag="VMZ33_04865"
     CDS             4991..>5415
                     /locus_tag="VMZ33_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HUR16593.1"
                     /translation="MTKRTPDGRSTDELELAPPPDAGPGADLEPSEESADPRARAARR
                     TAPDEAVLNDAPHAAEWFSFRGAAGPVARGIDEETQADRVALAERIMTRLNPEQARAV
                     TTTHGPLLILVGAGSGKTRVLAHRVAYLIGVKGVKPWQIL"
BASE COUNT          929 a         1595 c         1884 g         1007 t
ORIGIN      
        1 ggagccggtc acggccacgg cggagacatg gactactagt ccaatccctc agcactgaca
       61 ggacccgggt cgagcaatcg gcccgggtct tttctattgg cggaggccga tagggcctgc
      121 gcgctcatgc cagaactcga cgagttcgtc ccacgtgtgc gcttcggcgc ggttggagag
      181 atagccgatg acgaggaact catggctcgt ccactcgagc acgtggtaga gagcacccga
      241 cgaggacagg tgctcgcggc aattcagacg gcccaaactt tcaccttcat cgaacgagta
      301 atcggtcaag tgcctgccgc tcgcgcagtc cgttgacttg ggatcaggtg gagtctcggc
      361 accggcgacg tcctcgtcga acgcggcgtt catctcgtca ggggttacga acagcgaata
      421 gtcgaaagtc aggtcgtcgg gcccacatgt gaccgaggca atctcgccgt cgtatatctc
      481 gtcccggatg aagcatgtgc tgcgcagtcc ggcatgagcc aggctgatca ggtagtcggc
      541 tggtgtggtc ggctcgggtg gcgccgacag gctcggcgcc tcgattgtga ccggagtgag
      601 cgttggcgtc gggctggcag gccgcagggg tggggcaact gcggtggtgg gtgtggccgc
      661 ggcgggcggc tgtgtggtcg ctgaaggggc gaccgctccc ggcaccgccg acgaacaggc
      721 cgcggccagg gcgcacagca caacagaggc gacgtgacga accgccatgc cggagatgtt
      781 agtcatggaa gaaggccccg accagacggt cggggccccc tttcaccaga cgaacggact
      841 cgggaggcta ctcgattgcg gtcaccaccg ggatcgagac ggcgaccgtt tccggcgccg
      901 tgtacgtgac ctggttgagg atcgcgtcgc caacggcacc attgacggcc accttgacgg
      961 tccgcgtgcc tgtgtagcca acgttgaccg ttccgaggct ccaacgcacg gttcgcgttg
     1021 aggcgttgta ggtgccgctg ttgctggccg agacaaacgt caagccactc ggcaggacgt
     1081 ccgtcaacac ggcggacgaa gactcgttcg ggccgacgtt cgtgtacgtg gcggtgtacg
     1141 tgatctgcga cccgctcgcc gcggtggccg gtgcggccag ggtcaccgcc acagccgggt
     1201 cgcgtgcagg aaggctaggt gtcgagctga acacctggag ctccgccgcc ttgacgtcaa
     1261 ggtcggcgtc cgagccggtc acgcagtcac tgtcattgag cggatccgcg tcctggtcgc
     1321 cgcggaagcc ggtgccagtt gccgtgcact ggttcgagac gacgcgcagc tggacgtgcg
     1381 tggcgttcgt gtcaggcacg tcgaacgtgc gcaggatgag gtccggaacc gtgggccgcg
     1441 gtcgaacgcc agggaaggca ttggccgggc tcgtgtagat cttggtgaag ttcgtattga
     1501 ctgcgcacgt cgccgcggac ccgttgcaag tccagatctc gaattggcgg agcgctcgga
     1561 agcggccacc gttttcgggg ttgagcatcg cgctcacgtt gacgcgcttg atgttctgga
     1621 caccgccctg caggtccacc gtcgcgaggg ctgcgccggc tactaccggt gatgggcccg
     1681 tccagttcgt cagctcggtg tcatcgaaca ggttgttgaa gttcgtgcca gtgccagtcg
     1741 cgacggcgcc cttggtgagc gatgcgcggt tggtcgacat cctgatctcg acggttacgt
     1801 tgccggttcc gacgatgttg cgcgggaagc gtacgtggcc gtagcccggc gccgcgacga
     1861 agaactcgta ctcaccgttg acgaacttgg ccgtgttggt gagcgctcca gcaggaatgg
     1921 tgtccgcgat cggatttgcg gccgcttcga actgaccgac atagaccttg gcattcgtga
     1981 tgagtgcgtt gccctcatcg gcggccacga ccttgaaggt cacgctcttg aactgctcca
     2041 gcggtgactg gaagtctggc ttgggatcga catcggacgc accggcggac gaggcgttca
     2101 tgcccatgcc gcgatgcgcg aacacgttcc acagcagctt ctgatttgcg ccgttgaagc
     2161 ggatcatgtc ggccgcgaga tacgcgtcgc gggcgtccag catgctcaca gccgcaggca
     2221 tcagcagcca tgcgtcgaac atgatctgcg cccagcgccg attgcccggg cacgaactgg
     2281 ccgggtaggt gccctgtgcg caggcgcgct gccggctgtc gttggacgcc gggtagctgc
     2341 cgttgtactt ggcaatgaag gcctgacgca gatcgaagtt ggtggcggac cagatctcac
     2401 cgtcggcgtg cacctgggtg cgcccatcgg ggcacgtgcc ttcgatatcc gggcccagaa
     2461 ttgtctggtt gcacacgaag tcgtagccga cgtcgctgta gttcagcgga ctcgtgctca
     2521 tgttgaagtt gcggatgccg cgttggttgt tgccggtcgc atacgcgccg acggcatagc
     2581 ggctttcgcc attcgttgga acgaggccaa actcgttcag gtactccatg gccatcaggt
     2641 cgctccacga ctcacccatg gctcgagcct gggtgccgct gaggttcgaa tcggggccgc
     2701 cggccatgcg gttcgagatg gcgtggccgt attcgtgtcc gatgacagcc atgtcgtagt
     2761 caccatcgac gcacggtgag tagaagccgc cggcgatggg ctgccagaga tacatgttgg
     2821 taatgcccgg cacaccatcg ttgagagtcg tctggttggc gttgtcgcga gagacgccgg
     2881 cctcgactgg aagcagagcg ccggactgcg cctgtccaaa ctccgggtcg ttctcggcca
     2941 acgggccggg gttattgccg aagttgttgg tttggaggtt gtaggcgtct tccgtgaacc
     3001 cgaggaagta cgaccagtcg tgcatccggt tgtgggcgac gaagaggttg gttaccgcag
     3061 cgtcgatgtc gttgccaccc ggaacgaggt tcgttgtggc gcagcgtgat tggttccagg
     3121 cgttggtcca ggggaaggaa tagtcgcggt cgagcgatac cgggcgctgc tgcaatggtc
     3181 ctggggtgag cggggtcgtc cacgcctcac cggagatggc attgttgcca atcgtcgtga
     3241 acgacggcgc gttggtctgg acgatgtggt cccacggaac acgggaagcg aggttctgaa
     3301 gctttccaac cgaggttgtg cacccgggca cgttcccacc gttatgaacc aggacccagc
     3361 agccgattac acgagtgtcg gtgttggcaa ggtcgtagtc ctcgggcgag gggtcgttgg
     3421 ccagcgaggg atgcgcgcgg aagtgcttcc acttcggcgg atagttgaac tgggctgcgg
     3481 acgtgtcggc gtcgttggtc gcaaaggcgc cctcgtagtt gaacggctcg agcggatcgt
     3541 cggccgcgaa ctggcacact cgtgcgtagt acgtcgccgg cgctgggagc ggcggcgcga
     3601 tctcgtagtg gatcgcctcg gggctcgtgc cgctgtccat gttcgcgacc tcgaccagag
     3661 ccgagttgta gagatagaga acgatgtcgt tggcgggaat gacggcgctg gcagcgaccg
     3721 tgatgctggt cgtgcccgtc ggtgcgacga tcgcatggtc aggcccgcat tggccgtttg
     3781 cgtctgttgt gccgctgaat tggccgaacg tcggctcgtc gttggtgccc gtcgaaacct
     3841 ggaagtcatc ctgttgatcg aggtgatcaa ctcggttgta gcgcatcagg atcgaaccgg
     3901 accgggcgtc cacgaagacg gtgtatgcga gcggctcggc gcctgacgtg tcgagcacga
     3961 tcgtctcgaa ggcgacgacc gcgcgatcag agttgggcgt cggcactgcc acgagtcgcg
     4021 ctcgctggtc gaccgcgcct gagctcttgt cgttgtaagt gggtgtccgc agggcagtag
     4081 cggagaagac ggaccagtcg ccgactgtgc ggaccgcgcc gagtgccccg ccgaccgtgg
     4141 gcttgcccac gtgcgcggcg gccttcagcc aggcttgttg agcggacagg atcgcgctct
     4201 gtggcgcgga cttggaacca gtcgctgacg aagacgcgta agcgaccttg ccgccgatga
     4261 tggccacggt gatgaggcca tcctgcgcgg ccttcaaggt gccgtagcgc tgtcgaaaca
     4321 gaattgcccg tccctgcccg atggggttgg acgacaccag ctcaaggttc gtcacatcgg
     4381 tgtccgtcaa gcggaacaag gtccggttcg tccggatcca gttccgggct gcagtctcag
     4441 gcgtgccgct gaggccagtg gccagccagc cactgcgctt tatcagcgat tggggagttc
     4501 cccatctgtt ccagcgcacg ctcgcgccca actgcgtgac tcggctgatt tgggccgagg
     4561 tcggctggac cgaaccggct cggaagtcct tatcagacag ccctccgaga cccggcgtga
     4621 ccgcgagggt cgaggctggg gcagccaagg ctaggagcag gagcacctgg attccgacta
     4681 tgactcgacg gaatgcgagg ggacgggttt gggcgcgcat cgtgtcttct ccggatttaa
     4741 acgccacgac tcgggctggg acaccacagc aaggggtggc agtcggaagc atgcgccgaa
     4801 agtacgtggg cgcatagtct gacgaagccg tgaacctttc aaatggattt caactcgcta
     4861 ccccttggaa gcgagctgaa ccgactatcg tgcgggagcg cgtccccgaa caagcagctt
     4921 tggacgacag aagttccgcg tcggtagctt cgcaggggct ccggccatcc gctcggtaac
     4981 atcggaccga gtgacgaaga ggactccaga cggcaggtcg accgacgagc ttgagctcgc
     5041 accgccgccc gacgccgggc cgggcgcgga ccttgagcca tctgaggaat ctgcggatcc
     5101 ccgtgcacgg gcggcgcggc gtacggcacc agacgaggcg gtgctgaatg atgccccgca
     5161 cgccgcagag tggttctcat tccgtggcgc ggcgggaccg gttgctcggg gcatagacga
     5221 ggaaacgcag gccgaccgcg tcgctctggc cgagcggatc atgacccggc tcaaccccga
     5281 acaggcacgc gccgttacca cgacgcatgg gccgctgctg atccttgtcg gggccggctc
     5341 gggcaagaca cgcgtattgg cgcatcgagt cgcgtacctg atcggcgtca agggcgtcaa
     5401 gccgtggcag atcct
//
LOCUS       DATLCO010000199         2145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4014968, whole genome shotgun sequence.
ACCESSION   DATLCO010000199 DATLCO010000000
VERSION     DATLCO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4014968"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VMZ33_04870"
     CDS             complement(<1..429)
                     /locus_tag="VMZ33_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16594.1"
                     /translation="MTVAGPFHFESPGTRRRVLRTIAVVAPMLWILAPAGSRASVEAP
                     LPTSLVAEPFSTGGVRVIVDASRDADGVARICIEALALTEDGPEFQTAVYETACPSEM
                     RAKSSYRFALVRRVASISGRVHTVRIIERRQRQADGEWVGL"
     gene            584..1204
                     /locus_tag="VMZ33_04875"
     CDS             584..1204
                     /locus_tag="VMZ33_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16595.1"
                     /translation="MSNRRIALAGAGIVSAFATFSAFSVVRWFLTDQGHLPFASGSHL
                     IDSFRGEIAVALVAVGVAVGITALGIRRRLDVPTKIAAAVAVLLSSGAALAVYPSTTN
                     AELWVLEGNSGQPRWHLDLGVQLVYGVRRETRDALVLQVGTRPEHTCSFELLALTVDL
                     RRHRIASRRSLPTFYANEADLPPSPQPLNPSRYRVTQSKPATVCSE"
     gene            1306..>2145
                     /locus_tag="VMZ33_04880"
     CDS             1306..>2145
                     /locus_tag="VMZ33_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HUR16596.1"
                     /translation="MSISKKVRSVVSGPRDDNHDDMGDGARAARPRSVLAARVVGRDH
                     ELAALRDAVGAATHGGGRVVFLVGEAGIGKSRLAQVTARDAEQRGLRVLRGRAVPTAT
                     PVAYRPLAEVLSSAVRAGTAPDAAELAPFRAALGRLVPAWRDDQVADVDDSVFVLAEG
                     VLRFLRGVARSDGCLVVLEDLHWADPETLTILEYLADNLADENVVCVATVRDESPSPG
                     LDLARTLHSRRASDLIELRRLADVDVAEMLESCLGSDEALPELMALAARADGVPFLVE
                     ELLA"
BASE COUNT          318 a          741 c          710 g          376 t
ORIGIN      
        1 caaacccacc cactcgccgt cagcctgccg ctgccggcgc tcgatgatgc gcaccgtgtg
       61 gacccggccg gaaatcgacg ccacccgtcg gacgagggcg aagcggtacg acgatttggc
      121 gcgcatctcg gaggggcagg cggtctcgta cacggcggtc tggaactctg gcccgtcttc
      181 ggtgagggcg agcgcctcga tgcagatcct ggccaccccg tctgcgtcgc ggctcgcgtc
      241 gacgatcacc cggacgcctc cggtggagaa gggctcggca acgagggacg tgggcagtgg
      301 agcctcgacg ctggcgcgcg acccagccgg ggcgagtatc cacagcatcg gggcgacgac
      361 ggcgattgtc cgaagcactc gtcgccgcgt ccccgggctc tcgaaatgga aaggaccggc
      421 gaccgtcact tggacgaatt agacacgccg cgcgctcatt gcgtcgtgac caacgtcacg
      481 cggccgatgc ggcaacttct gcttcgcgct gcaaccgaca tccacggcca cctctgtcag
      541 actgcgcgtc gtggcgcggt ggcgtcgaga ccgcggaggt gttgtgagca atcggcgtat
      601 cgccctcgcg ggcgctggca tcgttagcgc gttcgcgacg ttctcggcat tcagcgtcgt
      661 tcgctggttc ctcactgacc aaggtcatct gccgttcgcc tccggttcgc acctgatcga
      721 ttcgtttcga ggtgagatcg cggttgcact ggtcgccgtc ggtgtcgcgg tcggcatcac
      781 ggcgctaggc atccggcgcc gcctcgatgt cccgacgaag attgccgcgg cagtggcggt
      841 tctccttagt agcggcgcgg cgcttgccgt ctaccccagc acaaccaacg ccgaactgtg
      901 ggttctcgag ggcaacagtg gccagccgcg ctggcacctg gacctcggtg tccagctcgt
      961 ctacggcgtg cgccgcgaga cgcgagacgc gttggtgctc caggtcggga ctcgaccgga
     1021 acacacttgt tccttcgagt tgttggcctt gaccgttgat cttcgccgcc accgcatcgc
     1081 cagtcggcgt tctttgccaa cgttctatgc gaacgaagcc gatctcccac cgtcgccgca
     1141 gccgctcaat cccagccgtt atcgcgtgac gcagagcaag cccgccaccg tctgcagcga
     1201 atgaacgtcc acctgcgtag gcggtcactt tggctcctcc acgaggcagg gcaccacttg
     1261 cacgacattc agcagatcgc tttgaccatc ttgccgatct gaggtgtgag tatctcgaag
     1321 aaggtccgct cagttgtcag cggcccgcgc gacgacaacc atgacgacat gggcgacggc
     1381 gcgcgagcgg cacggccgcg atccgtgctc gccgcccgcg tcgtcggacg ggatcacgag
     1441 cttgccgccc tccgtgacgc ggtgggggcc gccacacacg gaggcggaag ggtcgtgttc
     1501 ctggtaggcg aggcgggaat cggtaagtca cggctcgccc aggtaactgc gcgcgacgcg
     1561 gagcagcggg gcctacgagt tctgcgcggg cgcgccgttc ctacggccac gccggtggcg
     1621 taccgcccgc tggccgaggt gctcagctcc gccgtccgcg ccggcacagc gcctgacgcg
     1681 gccgagctcg cgcccttccg tgcggcctta ggccggcttg tgcccgcctg gcgcgacgac
     1741 caggtagccg acgtcgacga ctccgtgttc gtgctggccg aaggtgtcct ccgatttttg
     1801 cgcggcgtcg cccgctccga cggctgtctg gtggttctcg aggatctgca ctgggccgat
     1861 cccgagacgc tcacgatcct cgagtacctg gccgacaacc tcgccgacga aaacgtcgtc
     1921 tgcgtcgcca ctgtccggga tgagagtccc tccccaggcc tcgacctcgc ccggacgctg
     1981 cattcccggc gcgcgtcgga cctgatcgaa ctgcgccgtc tcgccgacgt cgacgtggcg
     2041 gagatgctcg agtcctgtct cgggtccgac gaggcgttac ccgagctgat ggctctcgcg
     2101 gcgcgcgccg acggcgtgcc cttcctcgtc gaagagttgc tggcg
//
LOCUS       DATLCO010000200         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_298213, whole genome shotgun sequence.
ACCESSION   DATLCO010000200 DATLCO010000000
VERSION     DATLCO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298213"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..446)
                     /locus_tag="VMZ33_04885"
     CDS             complement(15..446)
                     /locus_tag="VMZ33_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16597.1"
                     /translation="MGFEQELRSTSDEMMATLDQLARLESEKRVEQPGTPRFVKLAAE
                     VERVAALVFAQTSGQRVLAERSAVEVSAGGNLQPIDEVPALRDVSVILADWREAERVL
                     ATSTIETAEHAKAVADVRRFRDEYHRAHQSQRLPGSGTTQG"
     gene            545..2125
                     /locus_tag="VMZ33_04890"
     CDS             545..2125
                     /locus_tag="VMZ33_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HUR16598.1"
                     /translation="MSNDLRVLRAALLMAALVATLVGCVPTATPGQGGEPDVRIGAAE
                     PLRWDPAAAGDAGSGNTLAQIFEGLTAFDVNSNVQPALADSWLASDDGRQITFHMRPG
                     IRYSDGSAIVAQDVVDSWLRLIDPAAPSPLASLLDDVVGATDYRSGAADASGVGLRAD
                     GDNVVVDLRRAATYFISATASPSLAVVPPSMHGRLTSGPPANIVVSGAYVPSVTDAEY
                     IRLTGNPNYWAGLPAMDVVDLVIDYGNKTSIAAFDDNTVDYTGISSFDASWIAYDRDL
                     GSQLRLTQDFNVSYYGFDTTVPPFDRAEVRLAFAKAVDWARIVRLSDQTPATSMVPLG
                     VPGRDASEHRPTHDPDAARELLSQAGYPNGENFPRVLLATYGVGFEGTVAAELEANLG
                     VQVDVEVLDFSDYINREHGPGSPGIWTLSWSADYPHPHDFLGLLLETGSSSNDGQWSN
                     AEYDILLAEAAATANLDEQAQIYAQAQEILELEAPVVPLAYGESWALSREGLLGASEN
                     GVGILRIAGLAWAEGSGR"
     gene            2122..>3015
                     /locus_tag="VMZ33_04895"
     CDS             2122..>3015
                     /locus_tag="VMZ33_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HUR16599.1"
                     /translation="MRTARVLLLLTVVLATLAAPPAATAEPAFGQPSASAPLGQPVVV
                     TSTITAVEEGSTVEVLLGMDGHEQKIVVPAAPSGNAGKWTATAELDVPSAVECTCYAD
                     GHSAPNSPIQFQFRVRAADGRLTFGPVGNVLVADDRFTWRTLEQGLVRVHWYEGDQAF
                     AQTAADVANGAIDRAAELLGSTLPEPVDLFIYATQDALLEAVSPARENIAGEAHAQIQ
                     TMFVWIGPNDDRTESDVTIAHELTHLVFNEATFNQYHEPSRWLNEGVAVYLSEGYSNS
                     FRGVVEVSALTGQLIPLQGLAG"
BASE COUNT          501 a         1015 c          967 g          532 t
ORIGIN      
        1 tacgcacgca cggatcagcc ctgggtggtt cctgaccccg gcaggcgctg cgactggtgt
       61 gcccggtgat actcgtctcg gaaccgccgc acgtccgcaa ctgccttggc gtgctccgcc
      121 gtctcgatgg ttgatgtagc gagcacgcgc tctgcctcgc gccagtcagc gaggatgacg
      181 gagacgtcgc gcagcgccgg cacctcgtcg atgggctgca agttcccgcc cgcgcttacc
      241 tcgacggccg acctctccgc caagacgcgt tggccgctgg tctgcgcgaa gacgagcgcc
      301 gccaccctct ccacttcggc cgcgagcttt acgaatcgcg gcgtgccggg ttgctccacg
      361 cgcttctcac tctccagacg agccaactgg tcaagtgtgg ccatcatctc gtccgaagtg
      421 gaccgcagtt cttgttcaaa tcccattgcc ggccccctca ccgctggcac ccccgccagt
      481 acatacctga gtaggctaca ccgcggccaa gggcgggccg ggaactcgac tagactcgcg
      541 acccgtgtcg aacgacttgc gtgtgcttcg ggccgcactc ctcatggccg cgctggttgc
      601 aaccctcgtt ggatgtgttc ccacggccac gcccggacag ggcggcgaac ctgatgttcg
      661 cataggcgct gctgagccac tccgttggga tcctgccgcg gcgggtgatg ccggatcggg
      721 caacacgctc gcccagatat tcgagggact gaccgcattc gacgtcaact cgaacgtcca
      781 gcccgcgctt gcggactcgt ggctcgcctc cgacgatggt cgacagatca cctttcacat
      841 gcgacctggc atccgctaca gcgatggctc ggcaatcgtc gctcaggacg tggtcgactc
      901 ctggctccga ctgatcgacc ccgcggcgcc gtccccgtta gccagcctcc tcgatgacgt
      961 ggtgggagcg accgattacc gcagtggtgc ggccgacgcg agcggggttg gactgcgcgc
     1021 cgacggtgac aacgtggtgg tcgacctgcg ccgcgcggcc acctatttca tatcggccac
     1081 agcctcgcct tcgttagccg tggtgccgcc gtcaatgcac ggccgactga ctagcgggcc
     1141 gcccgcaaac attgtcgtgt cgggggcata tgtgccttcg gtgacggacg ctgagtacat
     1201 ccgcctgaca ggcaacccaa actactgggc tgggctgccg gcaatggacg tggtcgacct
     1261 tgtgatcgac tacgggaaca agacgtcgat cgcagctttt gacgacaaca cggtggacta
     1321 caccggcatc tcgagcttcg acgcgtcctg gatcgcctac gaccgcgacc ttgggtcaca
     1381 gctgcgcctc acgcaggact tcaacgtgag ctactacggc ttcgacacga cggtcccgcc
     1441 cttcgatcgt gcggaagtca ggcttgcctt cgcgaaggcc gtcgactggg cccgaatcgt
     1501 tcgcctgtca gaccagacgc cggcgacgtc gatggtgccg ctcggcgtgc ccggtcgcga
     1561 tgcgagcgag caccgcccga cccatgatcc ggacgccgcc cgtgagctcc tgagccaggc
     1621 cggttatccg aacggcgaga acttcccgcg agtgctgctt gcgacttacg gggttgggtt
     1681 cgagggaacc gtcgcggccg agctcgaggc gaaccttggc gtgcaggtcg acgtcgaggt
     1741 gcttgacttc tccgattaca tcaaccgcga acatggcccc ggctcgcccg gtatctggac
     1801 gttgtcctgg agcgccgatt atccccaccc acacgacttc ctcggcctgt tgctagagac
     1861 cggcagctcc agcaatgatg gtcagtggag taacgccgag tacgacattc tcctcgccga
     1921 ggccgcggca accgccaacc tggacgaaca ggctcagata tatgcgcaag cgcaggagat
     1981 tctcgagcta gaggcgcccg tcgtgcccct cgcttacggg gagtcgtggg cgttgtcgcg
     2041 ggagggcctg ctgggcgcat cggaaaacgg tgtcggcatc ctccgaatcg ccggcctcgc
     2101 ctgggcagag gggagcggcc ggtgaggacc gcccgcgttt tgctgctcct cacggttgtg
     2161 ctggcaacgc tggctgcccc accggcagcg accgcggagc ccgcattcgg ccaaccatcg
     2221 gcgagcgccc cgctcggtca accggtggtg gtgacgagca cgatcacggc ggtcgaggag
     2281 gggagcaccg tggaagttct cctgggcatg gacggccacg aacagaaaat cgtcgtcccc
     2341 gcggcgccga gtggcaacgc cggcaagtgg accgcgacgg cggagctcga cgttccctca
     2401 gccgtggagt gcacgtgcta cgccgatggc cacagtgcgc caaattcgcc catccagttc
     2461 cagttccggg ttcgtgcggc cgacggcagg ctgacgtttg gaccggtcgg caacgtcctc
     2521 gtcgcggacg acaggttcac ctggcgaacg ctcgaacagg gtctggtccg cgtccactgg
     2581 tacgagggcg accaggcctt tgcgcagaca gccgcggacg tggccaacgg cgcgatcgat
     2641 cgcgcggcgg agctccttgg ttcaactctc cccgaaccgg ttgatctctt catctacgcg
     2701 actcaggacg cgttgctgga ggctgtgtca ccggcgcgtg agaacatcgc cggcgaggcg
     2761 cacgctcaga tccagacgat gttcgtgtgg atcggcccca atgacgatcg gaccgaatcg
     2821 gacgtcacca tcgctcacga gctgacgcac ctggtgttca atgaggcgac gttcaaccag
     2881 taccacgagc cctcgcgatg gctgaatgag ggtgtcgccg tctacctctc ggagggttac
     2941 agcaactcgt tccggggcgt ggtcgaggtt tcggcgctca ccgggcagct catcccgctg
     3001 cagggcctgg ccggc
//
LOCUS       DATLCO010000201         8103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7603142, whole genome shotgun sequence.
ACCESSION   DATLCO010000201 DATLCO010000000
VERSION     DATLCO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7603142"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VMZ33_04900"
     CDS             <1..412
                     /locus_tag="VMZ33_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HUR16600.1"
                     /translation="VDGAHIRTLLMTFFYRHMPEVVGSGHLYIAQPPLYRISTGKVTR
                     YAKDDADRERIIKEFKSKNLSIQRFKGLGEMNADQLWETTMSPATRTLLRADVDEEGA
                     GETDRIFSALMGEKVEPRRDFIKARARQVQNLDI"
     gene            427..1413
                     /locus_tag="VMZ33_04905"
     CDS             427..1413
                     /locus_tag="VMZ33_04905"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HUR16601.1"
                     /translation="MPSAVTERAQAFVSERLPQARGLGVALADVLDDPDAFTATLREG
                     LQRLSDPEYEHEQERVAPGFGDVLGVRNPLMAAIARQIREPLRRISSTWALALAERLI
                     AEDEREIVLFSHVCLRRALPNDPEQSWQLMRRLARRAHDWIGVDELAELYAYGILLEQ
                     FRWAELEQLVFSLDKWERRLVGSTIARLPFELPKHQRSALGATSALTLIKSLLGDADP
                     DVQKALSWALRSWNEVDSPAVEAFLRVEAVNARSTDDGHSAWVLRDALTWPGTPLALQ
                     SAVRATLQGVRRRPNAPSTSAAASIAGNFIGLDRMAEAAVAMQGDRQRMGRG"
     gene            1417..3969
                     /gene="gyrA"
                     /locus_tag="VMZ33_04910"
     CDS             1417..3969
                     /gene="gyrA"
                     /locus_tag="VMZ33_04910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HUR16602.1"
                     /translation="MAEETGHIIPIRIEDELKVSYLDYAMSVIVSRALPDVRDGLKPV
                     HRRILYTMHEMGLTSGASYRKCAAIVGEVMGKYHPHGDVALYDALVRLAQTFSLRYPL
                     IDGQGNFGSVDGDPPAAMRYTEARMTAIAAEVLADIDKQTVDFVENYDGTRMQPSVLP
                     AKLPNLLVNGSAGIAVGMATNIPPHHLGEVVDATIALIDDPNLTNDELCNIVTGPDFP
                     TGGTIFRFDRRRNSITGQWEEVDAIRDMYAHGRGRIVMQAVVSFEETRQQRTAIVVTE
                     LPFQVNKASLAEKIADLVQGKKIEGIADIRDESDRDGMRLVIECKRDAAPRKVLNNLF
                     KHTALKMAFNMNMLALVDGQPQTLPLKQVLAHHIDWRREIVRRRTEFDLAKARDRAHI
                     LEGLKIALDNLDAVIKTIRESADVETARANLISRFELSEIQANAILEMQLRRLAALER
                     KKIEDEYLATIKLIAELEDILANPARVLAIIKDELRELSAKYAGERKTRVEGDADREL
                     TDEDLIADEDVVVTVSTRGYIKRQPLATYRRQVRGGKGIIGARTVEEDAVAHLLVAGT
                     HDWVLFFTNRGRVFSSKVHAMPTPAERTSKGIPVINLEGVQLDPGELVLAIITIPNFE
                     KGDNLVMATRHGVIKKTPIEQFERVRSTGIRAITVAEKDELAWVGVSSGNDDIILASA
                     MGKIGRFSEKEVRPMGRDAAGVIGIRLARQGDRVVSMRVVKPDDDILVLTETGYGKRV
                     ALSDFRRKHRGGQGVTLIPIEGGKTGPVAAVELVDETAEELLLISKGGQVVRTDVKSV
                     NRYGSQARGVTVMRLNEGDEVAGISVFRADEREAVEANPIDGEGSPEPPAAG"
     gene            4068..5126
                     /locus_tag="VMZ33_04915"
     CDS             4068..5126
                     /locus_tag="VMZ33_04915"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HUR16603.1"
                     /translation="MTAFGSRAATNYSSEQFDLYTANANPALAQKIARYLGRDLGRAE
                     VFEFANENIFVRIEDNARDRDVFIVQPTNSPVQKSIMELLIMIDAFKRASAGRITAVM
                     PYYAYGRSDKKDQPRVPITARLIADMISVAGADRVLTMDLHQGQIQGFFNIPVDELTA
                     VHMLSNYFKRLNLTNLVVVTDLGFAKRARTFAEILEAPLAVIEKRRPGTGAAPADLGK
                     AELLNVIGEVKGRTAIIVDDEIDTASTLMEIERALMREGVTEIFACATHGVLSDPATD
                     RLRTSEITEVVITDTVPLPPHKHLPKIKVLSVAPLIGEAIRRIHRGESVGALFSSDLE
                     LVQEMLLWDDADSENHVA"
     gene            5243..5482
                     /locus_tag="VMZ33_04920"
     CDS             5243..5482
                     /locus_tag="VMZ33_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16604.1"
                     /translation="MSLKLYRPTPDGLEPHPVEHKTWRSQLRSRRWQPAELANPEFKP
                     TSNRVAVLFWLSLAALTFVIILVGYGLNIWTLPPA"
     gene            5536..>8103
                     /gene="secA"
                     /locus_tag="VMZ33_04925"
     CDS             5536..>8103
                     /gene="secA"
                     /locus_tag="VMZ33_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HUR16605.1"
                     /translation="MRNFISRFVDSNDRNVKKLHPIVDEINSLEQEFQALTDDQIRDR
                     MVALRAEIMSESAETPPSEEELSAESSERRRELRKERRKGDLDHLQDVLDDALPEVFA
                     AAREVGQRKLGMRHFDVQMLGGIVLHQGKIAEMKTGEGKTLVAPLAAALNAMSGLGVH
                     VVTVNDYLAKRDPQWMGPIFHGLGLTVGIIQHDSAFIFDPEHRGTDERLLNLRPVERR
                     QAYAADVTYGTNNEFGFDYLRDNMVTELSERVQRERYFSIVDEVDNILIDEARTPLII
                     SGQAEESEDLYYQFARLVPRLQPRPEGAEEGGDYFIDLKEHAVSPTEDGITKIERLLK
                     VDNLFDADPRLVRHFEQALKAHALYKRDKEYIVKEGEIVIVDEFTGRQMPGRRWSEGL
                     HQAIEAKEGLRVQRESVTLATITFQNYFRLYHKLSGMTGTAMTEHEEFYKIYGLDVIS
                     IPTHRAMVRADQADLVYRTENDKFTALIDEIVEMTEGGRPVLVGTTSVEKSEVLAEML
                     KRRGIKHEVLNAKFHEKEAPIVAQAGRLGSVTIATNMAGRGTDILLGGNPAGLASADL
                     HKRGINPAEAPPEVYEEALAKAKAQVTEEHDRVVEAGGLHIIGTERHEARRIDNQLRG
                     RAGRQGDPGSSRFYLSLEDSLMKRFASDRVAGLMTRMGLEGDVALESRLVSRTIESAQ
                     TRVEGYNFDIRKRVVEYDDVINKQRETIYAERDKVLRNEDLTETVRQFIDDELETLVD
                     GKLADPSPEEWDFDGLAHEIEQMGLGGDGLDAQGLADIAVRDDILERLREGVDTALEE
                     RETRYGAEVWAQIEKFVLLRAIDSLWVDHLTELDDMRRGIGLRGYAGIDPLNEFRREA
                     "
BASE COUNT         1557 a         2683 c         2562 g         1301 t
ORIGIN      
        1 cgtcgacgga gcccacattc gcaccctgtt gatgacgttc ttctaccggc acatgcctga
       61 ggtcgtcggc agcggccacc tctacatcgc gcagccgccg ctctaccgga tcagcaccgg
      121 caaggtgact cgctacgcga aagacgacgc cgaccgcgag cggattatca aagagttcaa
      181 gtccaagaat ctctccatcc agcgcttcaa gggcctcggt gagatgaacg ccgaccagct
      241 ctgggagacc accatgagcc cggcgactcg aacgcttctg cgggcggatg tcgacgagga
      301 gggcgccggc gaaaccgacc gcatcttcag cgctctgatg ggggagaaag tcgaacctcg
      361 acgcgatttc atcaaggccc gggcccgcca ggtccagaac ctcgacatct aggggatcag
      421 tccaacttgc cttccgcagt caccgagcgt gcccaggcgt tcgtgtccga gcgcctaccc
      481 caggcacgcg ggctcggtgt ggcgctggcc gacgtgctcg atgatccgga cgcgttcacg
      541 gccactctgc gcgagggact tcagcgactc tcagacccgg agtacgagca cgagcaggag
      601 cgagtcgcgc ccggcttcgg cgacgtttta ggcgtccgca atccgctcat ggcggcgatc
      661 gctcgccaga ttcgggagcc gctgcggcgc atctcctcta cctgggcgct tgccctggct
      721 gaacggctca tcgccgagga tgagcgcgaa atcgtccttt tcagccacgt atgtctacga
      781 cgggcgcttc ccaacgatcc cgagcagtcg tggcagctca tgcgccgcct ggcacgccgt
      841 gcccacgact ggatcggcgt ggacgagctc gccgagctct acgcatacgg catcctcctc
      901 gagcagtttc ggtgggcgga gctcgagcag cttgtgttct ccctcgacaa gtgggagcgg
      961 cggctggtcg gctcgactat cgcgcgactt ccgttcgagc taccaaagca ccaacgctcg
     1021 gcattgggcg cgacgtccgc tttgacgctc atcaaatcgt tgctcggcga tgctgacccg
     1081 gacgtccaga aggcgctctc atgggcgctg cgcagctgga acgaggtgga ttctcccgcc
     1141 gtcgaggcat tcctacgggt tgaggccgtc aacgcacgtt caacggatga tggccacagc
     1201 gcatgggtcc tccgagatgc cctgacgtgg ccagggacac cattagcgct tcagagcgcc
     1261 gtacgggcca cgctacaggg cgtacggcgc cggccaaacg ccccatcgac gagcgcggcg
     1321 gcgtcgatcg caggcaactt catcgggctc gatcgcatgg ccgaggcagc cgtcgcgatg
     1381 cagggcgatc gccagaggat ggggagaggc tgatcagtgg ctgaagagac cgggcacata
     1441 atccccattc gtatcgagga cgagctgaag gtcagctacc tcgattacgc gatgagcgtg
     1501 atcgtcagtc gtgctctccc cgacgttcgc gacggcctca agcccgtgca ccgccggatc
     1561 ctctacacga tgcacgagat ggggctcacg tccggggcga gctaccgcaa gtgcgccgcg
     1621 atcgtcggcg aggtgatggg caagtaccac ccgcacggtg acgttgccct gtacgacgcc
     1681 ctcgtccgcc tcgcccagac gttctcgctg cgttacccgc tcatcgacgg acagggcaac
     1741 ttcggctcgg tcgacggcga tccaccggcc gccatgcgct acacggaagc acgcatgacg
     1801 gcaatcgcgg ctgaagtcct tgccgacatc gacaagcaga ccgtcgattt cgttgagaac
     1861 tacgacggca cgcgcatgca gccctcggtg ctgccggcaa agctccccaa cctgctcgtc
     1921 aacggttcgg cggggattgc cgtgggcatg gcgaccaaca tcccgcccca ccaccttggc
     1981 gaggtggtcg acgcgaccat cgcccttatc gacgacccca acctcacgaa cgacgagctg
     2041 tgcaacatcg tcaccggtcc ggacttcccg accggcggca cgattttccg cttcgaccgt
     2101 cgccgaaact cgatcacggg ccagtgggaa gaggtcgacg cgatacgcga catgtacgcc
     2161 cacgggcgcg gccgcatcgt gatgcaggcc gtggtcagtt tcgaggagac gcggcagcag
     2221 cgcactgcga tcgtcgtcac cgagctgccg tttcaggtga acaaggcgag tctcgccgag
     2281 aagatcgcag acctcgtcca gggcaagaag atcgagggca tcgccgacat ccgcgacgag
     2341 tccgaccgcg atggcatgcg gctcgtcatc gaatgcaagc gcgacgccgc cccgcgcaag
     2401 gtcctgaaca acctgttcaa gcacacagcg ctgaagatgg ctttcaacat gaacatgctg
     2461 gctctcgtcg acggccagcc gcagactctg ccgctgaagc aggtcctggc gcaccacatc
     2521 gactggcgac gcgagatcgt tcgacgtcgg acagagttcg acctggccaa ggcgcgcgac
     2581 cgggcccaca tcctcgaagg gctgaagatc gcgctcgaca acctcgacgc ggtgatcaag
     2641 acgattcgcg agtcggccga tgtcgagacg gcgcgggcca acctcatcag ccgcttcgag
     2701 ctctcagaaa tccaggcaaa cgcgatcttg gagatgcagc tgcggcgctt ggcggcgctg
     2761 gagcgcaaga agatcgagga cgagtacctg gccacgatca agctcatcgc cgagctcgaa
     2821 gacatccttg ccaacccggc acgggtgctg gcgatcatca aggatgagct gcgcgagctc
     2881 agcgccaagt acgccggcga gcgcaagacg cgtgtcgagg gcgacgccga tcgcgagctc
     2941 accgacgagg acctcatcgc cgacgaggac gtggtggtca cggtctcgac gcgcggctat
     3001 atcaagcgcc agcccctggc cacttatcgc cgccaggttc gtggcgggaa gggaatcatc
     3061 ggcgcgcgga ccgtcgaaga ggacgccgtt gcccacctcc tcgtggccgg gacgcacgac
     3121 tgggttctgt tcttcaccaa ccgtggacga gtgttcagct ccaaggtcca cgcaatgccg
     3181 acccccgccg agcggacctc caagggcatc ccggtgatca acctggaggg tgttcaactt
     3241 gatccgggcg agctcgtcct ggccatcatc acgatcccga acttcgagaa gggcgacaac
     3301 ctggttatgg ctacccgcca tggcgtgatc aagaagacac ccatcgagca gttcgagcgc
     3361 gtgcgctcaa cggggatccg ggccatcacg gtcgcggaga aggatgagct ggcatgggtc
     3421 ggcgtgtcgt ccgggaacga cgacatcatc ctggcttccg cgatgggcaa gatcggccgc
     3481 ttcagcgaga aggaagtgcg gccgatgggt cgcgacgcgg cgggcgtgat cggcatccgc
     3541 ctggcacggc aaggcgaccg cgtcgtgagc atgcgcgtcg tcaagcccga cgacgacatc
     3601 ctggtgctca ccgagaccgg gtacggcaag cgcgtggcgc tgtccgactt ccgtcgcaag
     3661 catcgcggcg gccagggcgt gacgcttatc cccatcgaag gcggcaagac cggacccgtg
     3721 gccgctgtgg agcttgtcga tgaaaccgcc gaggaacttc tgctcatcag caagggcggt
     3781 caggtcgtgc gaacggacgt caagagcgtc aatcgctatg ggtcacaggc gcgcggcgtg
     3841 acggtcatgc gcctcaacga aggtgacgag gtggccggca tctccgtgtt ccgcgctgac
     3901 gagcgtgaag ctgtcgaagc caatcctatc gacggcgagg gctcgcccga accgcccgcg
     3961 gccggttagg ggctggtagg cgcgggtctg atgccgcaca atcgggttgg ctccgcagtg
     4021 gcactccgtg tctgctgcgc ttccttgcgg ccgcgggagg gccccagttg accgctttcg
     4081 gcagccgtgc cgcgaccaac tacagcagcg agcaattcga cctctacacg gccaacgcca
     4141 acccggcgtt ggctcagaag attgcccgct acctcggtcg tgatctgggg cgtgccgagg
     4201 tgttcgagtt tgccaacgag aacatcttcg tgcgcatcga ggacaacgcc cgcgatcgcg
     4261 acgtgttcat cgtccagccc acgaacagtc ccgtccagaa gtcgatcatg gaactcttga
     4321 tcatgatcga cgccttcaag cgcgccagcg ccgggcgaat caccgcggta atgccctact
     4381 acgcctacgg ccgatccgac aagaaggacc agccgcgcgt cccgattacc gcccgcctga
     4441 tcgccgacat gatcagcgtc gccggcgcgg accgcgtact gacgatggac ctccaccagg
     4501 gacagatcca gggcttcttc aacatcccgg ttgatgagct gaccgccgtg cacatgcttt
     4561 cgaactactt caagcgcctc aacctgacca acctggtcgt cgtgaccgac ctcggcttcg
     4621 cgaagcgcgc tcgcaccttc gccgagatcc tggaggcgcc gctggcggtc attgagaagc
     4681 gccgaccggg cacgggtgcg gcgcccgccg acctggggaa ggccgagctc ctgaacgtca
     4741 tcggcgaggt caagggccgt accgcgatca tcgtggacga cgagatcgac accgccagca
     4801 cgctcatgga gatcgagcgc gcgctgatgc gcgagggtgt caccgagatc ttcgcctgcg
     4861 ccacgcacgg cgtcctgtct gatccagcga cagaccggct gcgcacgtca gagatcaccg
     4921 aagtcgtgat taccgacacc gttccgcttc cgccacataa gcacctgcca aagatcaagg
     4981 tcctgtccgt ggcgccgttg atcggcgaag ccatccggcg catccaccgc ggtgagtcgg
     5041 tcggcgctct cttttcgagc gatctcgagc ttgtccagga gatgctgctc tgggacgacg
     5101 ccgactcgga gaaccacgtc gcgtgaatcg ccgatgtccg cgatgatccc cacatgatcg
     5161 accttcgccg gggggcctcc ggccccggct gcgcactccg gttgacaccc tgcatccgca
     5221 ggctgccgac cttcgtttgc caatgagcct caagctttac cgaccgacac ctgacgggct
     5281 cgagccgcat ccggtcgagc acaagacctg gcgcagtcag ctccgctcgc gccgatggca
     5341 gcccgccgag ctggcaaacc ccgagttcaa gccgacgagc aaccgcgtgg cggtgctctt
     5401 ctggctgagc cttgccgcgc tgacgttcgt gatcatcctg gtcggctacg gcctcaacat
     5461 ctggaccctg ccgccggcct gacacttctc cgctcaggcg gtcgaagcgg cacccagacc
     5521 tatacttcga aggctgtgcg caatttcatc tcgcggttcg tcgactcgaa cgaccgcaac
     5581 gtcaagaagc ttcatcccat cgtcgacgag atcaactccc tcgagcagga gtttcaagca
     5641 ctcacggacg atcagattcg tgaccgcatg gtcgctctgc gggccgagat catgtccgag
     5701 tcggctgaga cgccgccctc ggaagaggaa ctgagcgccg aaagctcaga gcgtcggcgc
     5761 gagctgcgca aggagcgccg caagggagat ctcgatcacc tccaggacgt cctcgacgac
     5821 gcgctgccgg aggtgtttgc tgcggcacgc gaggtcggcc agcgcaagct gggcatgcgc
     5881 cacttcgacg ttcagatgct gggcggcatc gtccttcacc agggcaagat cgccgagatg
     5941 aagaccggcg agggcaagac cctcgtcgca ccgctcgccg ctgcgctgaa cgcgatgagc
     6001 ggccttggcg tccacgtggt caccgttaac gattacctcg ccaagcgcga cccgcaatgg
     6061 atgggcccga tctttcacgg cctcggcctg accgtcggca tcattcagca cgacagcgct
     6121 ttcatcttcg atccggagca ccgcggaacc gacgagaggc tcctcaacct gcgccccgta
     6181 gagcggcgcc aggcttacgc cgccgacgtc acctacggca cgaacaacga gttcgggttc
     6241 gattacctac gcgacaacat ggtcaccgag ctctctgagc gtgtgcagcg cgagcgctac
     6301 ttctccatcg ttgatgaggt cgacaacatc cttatcgacg aggcccgcac gccgctcatc
     6361 atcagcggcc aggccgagga gtcggaagac ctctactacc agttcgcccg actggttccg
     6421 cggctgcagc cgcgccctga gggtgccgag gagggcggcg attacttcat cgacctgaag
     6481 gagcacgcgg tctcgccgac cgaggacggg atcaccaaga tcgagcggct gctcaaggtc
     6541 gacaacctct tcgacgccga tccgcgcctg gtgcgccact tcgagcaggc actcaaggct
     6601 cacgccctct acaagcgcga caaggaatac atcgtcaagg aaggcgagat cgtcatcgtc
     6661 gacgagttca ccggccgcca gatgccaggt cgccgctggt cggagggtct gcaccaggcg
     6721 atcgaggcca aggagggact gcgtgtccag cgcgagtcgg taaccctcgc caccatcacc
     6781 ttccagaact acttccggct ctatcacaag ctgtcgggaa tgaccggcac ggccatgacg
     6841 gagcacgagg agttctacaa gatctacggc ctcgacgtga tctcgatccc gacccaccgg
     6901 gcgatggttc gcgccgacca ggccgacctc gtctacagga cggagaacga caagttcacg
     6961 gcgctcatag acgagatcgt cgagatgact gagggtggac ggccggtcct cgttggaacg
     7021 acgagcgtcg agaagagtga agtgctggcg gagatgctca agcgtcgcgg catcaagcat
     7081 gaggtgctga acgccaagtt ccacgagaag gaagcgccca tcgtcgccca ggcgggacgc
     7141 ctcgggtccg tgaccattgc cacgaacatg gccggccgcg gcacggacat cctgctcggc
     7201 ggcaacccgg ccggccttgc gtcggcggat ctccacaagc gcggcatcaa cccggccgag
     7261 gccccgcccg aggtatacga agaggcgctg gccaaggcca aggcacaggt caccgaggag
     7321 cacgatcgcg tggtcgaggc gggcggcctg cacatcatcg gcactgagcg ccacgaggcg
     7381 cgccggatcg acaaccagct gcgcgggcgc gccggccggc agggtgaccc cggatcgtca
     7441 cgcttctacc tgtcactcga agactcgctg atgaagcgct tcgcctccga tcgcgtcgcc
     7501 ggactcatga cgcggatggg ccttgagggc gacgtggcgc tcgagtcacg tctggtatcg
     7561 cgcaccatcg agagtgccca gacccgtgtc gagggctaca acttcgacat tcgcaagcgc
     7621 gttgtcgagt acgacgacgt catcaacaag caacgcgaga cgatctacgc tgagcgcgac
     7681 aaggtcctgc gcaacgagga cctgaccgag accgtccgcc agttcatcga cgacgagctt
     7741 gagacgctcg tggacgggaa gctggccgat ccgagccccg aggagtggga cttcgacggt
     7801 ctcgcccacg agatcgagca gatgggcctc ggcggggatg ggcttgacgc gcaggggctg
     7861 gccgacatcg ccgttcggga cgacatcctc gagcgcctcc gtgagggcgt ggacactgct
     7921 ctcgaggagc gcgagacgcg gtacggcgcc gaggtctggg cacagatcga gaagttcgtc
     7981 ctgctgcgcg ccatcgacag cctgtgggtc gaccacctga ccgagctgga cgacatgcga
     8041 cgcggcatcg gactgcgtgg ctacgccggt atcgatcccc tgaacgagtt ccgacgtgag
     8101 gcc
//
LOCUS       DATLCO010000202         3865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3142738, whole genome shotgun sequence.
ACCESSION   DATLCO010000202 DATLCO010000000
VERSION     DATLCO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3142738"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VMZ33_04930"
     CDS             complement(<1..230)
                     /locus_tag="VMZ33_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR16606.1"
                     /translation="MATDAPAATRAIWGPDRRLAVQITDASKSFGAVMGVAGISLSVP
                     QSAIVGIIGPSGAGKTTTIRLITGSLAPDRGEV"
     gene            537..1703
                     /locus_tag="VMZ33_04935"
     CDS             537..1703
                     /locus_tag="VMZ33_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16607.1"
                     /translation="MSAMSSNEMYLTARAAGLRYVDRTDPGIRRVKRGDAFGFVDVAG
                     KAVRDAATLSRIKALVIPPAWTEVWICPTANGHIQATGRDARGRLQYRYHADWARVRD
                     ETKYERTLGFARALPRLRRRVTRDLALRGLPRDKVLATIVRLLEMTMVRVGNEEYARE
                     NKSFGLTTLRDRHVKVRGAELKLSFRGKSGKDHAVGIEDRRVARIVKACQDLPGQRLF
                     QYLDEDGNRQSVDSDDVNQYMRAAMGDEFSAKDFRTWAGTVLAARAFQALEAATGDEQ
                     LAEAAAAGVTNRGRSSLTRAVEEVAQQLGNTPAVCRKCYIHPQIIDAYMDGSLAEALT
                     RRAGEVLETDKGLRAEERFVLSILQRRLAADNRKAGRAARAAGAAGAKQSRKAA"
     gene            2274..>3865
                     /locus_tag="VMZ33_04940"
     CDS             2274..>3865
                     /locus_tag="VMZ33_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUR16608.1"
                     /translation="MIVATLALLAVGALAVVGGTATGLAFLDRMESELPDVSGFESLD
                     FAEPSVVYDRTGTIELARFQVERRQVVTYEEIPKILLDATIAVEDRTFWENEGFDPNA
                     IAVAVAESLSGGRERGASTITQQLVRARLLPQDVVEGDQVVRKIKEILQARNLTKAFP
                     GEDGKQRILTAYLNQIYYGHNAYGIAAAAEVYFGVPDLKLLTPAQAALLAGMPQSPDT
                     YDLFKWAERDATGQLVVPTQSENGEALPPPVERRNFILRTLEAGEGHFVRLTTAQLEQ
                     ALNEPITLHEEERIVFKAPHFVWYMKSQVDQLLADRAPIERGGYRIITTLDMGAQDLA
                     ERYINAGTVLPNLSEASMEQQISIQGLTQDAEWIRTLHNMDIHNGALVALDARTGDIM
                     AYVGSAGYYRDDLASPQLDPKFDVAGRGYRQSGSAWKPIVYAAGFDRGVVTPGTLLLD
                     VTTEFARDYFPGDADRKERGPVLMRDALTYSLNIPTIRALDRIGVETVAALASVMNIS
                     FPRGDRHLLQAGLAGAIGTVETT"
BASE COUNT          701 a         1260 c         1298 g          606 t
ORIGIN      
        1 acctctcccc ggtccggcgc cagcgaaccg gtgatgaggc gaatcgtcgt tgtcttgccg
       61 gcgccgctgg ggccgatgat gccgacgatc gcgctctgcg gcacggacag gctgataccg
      121 gcaacgccca tgaccgcacc gaagctcttc gaggcatcgg tgatctgcac ggcgagccgg
      181 cggtcgggcc cccagatcgc gcgagtcgcg gccggagcat ccgtcgccat ctggggcgtg
      241 gtgtgggtca gttccggcgc cacatcgggc ggtccaccaa gggactccgt ggccggcggc
      301 agcgtgttcg ggacaggctc aatcggtggg ttggggacag gtggagatag ctggtgagac
      361 gggtatgacg acacagcctg cgcatctttt gcgcttcgcg cttgcaccga cagacggcga
      421 gccgccccat ggcgatgcaa aggcatccca accgcgccgc tgttagcggg ctgcaatggc
      481 ggtaactcgg tctggtcgtg acgggtaccg accgcgctgg ctagggtcga aacgacatga
      541 gcgccatgag ttccaacgag atgtacttga cagcgcgtgc cgccggattg cgttacgtcg
      601 accgcacaga tccagggatc cgtcgcgtca aacggggcga cgcttttggc tttgtggacg
      661 tagccggcaa ggcggtgcgc gacgcggcca cgctgtcacg catcaaagcc ctggtgatcc
      721 ccccggcgtg gaccgaggta tggatctgcc cgacggctaa cggccacatc caggcgactg
      781 gccgcgatgc ccgggggcgg ctgcagtacc gctatcacgc cgactgggcc agggtccgcg
      841 acgagacgaa gtacgagagg accctcgggt ttgcacgggc cttgccccgg ctgcgacgcc
      901 gcgtgacgcg cgacctggcg ctgcgtggct tgccgcgcga caaggtcctg gcgacgatcg
      961 tgcggctgct cgagatgacc atggtccgcg ttggcaatga ggaatatgcg cgcgaaaaca
     1021 agtcgttcgg cctgacgact ctgcgtgatc ggcacgtcaa ggtgcgtggc gcggagctga
     1081 agctctcgtt ccgcgggaaa tccggcaagg accatgccgt gggaatcgag gaccggcgcg
     1141 tggcgcgcat cgtcaaggca tgtcaggacc tgcccggcca gcggctcttc cagtacctgg
     1201 atgaggacgg caaccgccag agcgtggact ccgacgacgt gaatcagtac atgcgcgccg
     1261 caatgggcga cgagttcagc gccaaggatt tccggacatg ggctggcacg gtgctggcag
     1321 ctcgcgcgtt tcaggcgctc gaggcggcga ccggtgacga gcagctcgcc gaggcggccg
     1381 cggccggggt cacgaaccgc gggcgctctt cgctcacacg agctgtcgaa gaggtggcac
     1441 aacagctggg caacacgccg gcggtgtgtc gcaagtgcta tatccaccca cagatcatcg
     1501 atgcgtacat ggatggctca ttggccgagg cgctcacccg tcgcgccggc gaagtgctcg
     1561 agaccgacaa ggggctccgg gcggaggaac gcttcgtgct gtcgatcctc cagcgacgcc
     1621 tcgcggccga caaccgcaag gcaggccgcg ccgcccgtgc cgctggcgcc gctggggcga
     1681 aacagtcccg caaggccgcg taacccacaa aaggtctccc gtgccgcccg gggcgcccga
     1741 cccgcaccaa gccgtgaggt ggggcgacgc gtgtcgtgtg gatctacgac gaagtcgcgg
     1801 tgcgtgtctc cgatcaacta ccgattccgc agttgggaat tgacttacga cttacggatc
     1861 cggggctctt cccgtggtgc ggttctgctt cgatattccc acgggacggg caatcaacag
     1921 cacactgagg tgccgagggc cttgaatccg cgccattccg tctcgttgcc ccatggggtg
     1981 ggaatgcagt gggagtgctt gtgcgtggag cgcccgacca agacggccaa cctgaggcgc
     2041 gcaacatgac gcaccgcgca ggcgtcaacc aagacacggc caggacatcg accggcaagc
     2101 gccgatcctg tggctgacgg acaatgcagg ttcccgtgta tcgaggtcaa caagcgcgcc
     2161 cacgccatcg caatggcgcc aggcagcgta aatccaacct aggccgactg gctctcgcgc
     2221 gcgtccgtgc gcgaccgaag ccaaagagcc gccttggcac gtggatcgca accttgatcg
     2281 tcgccacgtt ggcgctgctg gcggtgggcg ctcttgccgt ggtcggcggc acagcgacgg
     2341 gtctggcctt cctggaccgg atggaatcgg agctgcctga tgtatcgggc ttcgagtccc
     2401 tcgacttcgc cgaaccgtcc gtcgtctacg accgtaccgg gaccattgag ctcgcccgct
     2461 tccaggttga gcgacgccag gtcgtcacct acgaggagat ccccaagatc ctgctcgacg
     2521 ccacgatcgc ggtcgaggac cgcacattct gggagaacga gggtttcgac cccaacgcaa
     2581 tcgcggttgc cgtggccgag agcctgagcg gcggacgcga acgtggcgct tcgacaatca
     2641 cccagcaact ggtgcgcgcg cgtttgctgc cacaggacgt cgtggagggc gaccaggtgg
     2701 tacgcaagat caaggagatc ctgcaggcac gaaatctgac caaggcgttc cccggagaag
     2761 acggcaagca gcgcatcctc actgcgtacc tgaatcagat ctattacggg cacaacgcct
     2821 acggcatcgc cgccgcggcg gaggtgtact tcggcgttcc tgacctgaag ctcctgactc
     2881 cggcccaggc cgcgctcctg gcgggcatgc cgcagtcacc ggacacttac gacctgttca
     2941 agtgggccga gcgtgacgcg accggccagc tcgtcgtgcc gacccaatcg gagaacggcg
     3001 aggccctgcc accgccggtc gagcgacgca atttcatcct gcgcaccctc gaggccggcg
     3061 agggccattt cgtccggctg accacggctc agctcgagca ggcgctcaac gagccgatca
     3121 cgctgcacga ggaagaacgg atcgtgttca aggcgcccca cttcgtgtgg tacatgaaga
     3181 gccaggtcga tcagctcctg gccgatcgag cgccgatcga gcgcggcggc taccgcatca
     3241 tcacgacgct cgacatgggc gcgcaggacc tcgccgagag gtacatcaac gcgggcaccg
     3301 tcctgcccaa cctgtccgag gcaagcatgg agcagcagat ctccatccag ggcctgacac
     3361 aggacgccga gtggatacgg acgctccaca acatggacat ccacaatggc gcgctggttg
     3421 cattggatgc tcggaccggc gacatcatgg cgtacgtcgg gagcgcaggt tattaccgtg
     3481 acgacctcgc atcgccgcag ctcgacccca agttcgacgt cgcgggacgc ggctaccgcc
     3541 agtccggatc ggcgtggaag ccgatcgtct acgcagccgg cttcgaccgg ggcgttgtaa
     3601 cgccggggac gctcctcctg gacgtcacca ccgagttcgc gcgcgactac ttcccgggcg
     3661 atgccgaccg aaaggagcgc ggcccggtcc tgatgcgcga cgcgcttacg tactcactca
     3721 acatcccgac gattcgggcg ctggaccgca tcggcgtgga gacggtggcg gctctcgcca
     3781 gcgtgatgaa catcagcttc ccgcgcggcg atcgacacct gctgcaggcg gggctggcgg
     3841 gcgccatcgg caccgtcgag acaac
//
LOCUS       DATLCO010000203         4762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1053328, whole genome shotgun sequence.
ACCESSION   DATLCO010000203 DATLCO010000000
VERSION     DATLCO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053328"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VMZ33_04945"
     CDS             <1..1405
                     /locus_tag="VMZ33_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16609.1"
                     /translation="VSAEGRSVARIDDEAATVGRLAAVVEPLIEIHGQHDQQRLLQGT
                     WQRDVLDAFGGHADLRTTVMEAVRAMRSNEAALRELVVDPAELERRLELAAFAADEID
                     AASPREGEVDELRSGLAVAGNAQRIAALLSEAHGLLADEGRGARDALARAGQTTVELA
                     RLDPSAEDLATRLDGLAAELDEVVLDLRRREDGIAEAAADTESAEARLGVLYGLMRKY
                     GETEVALIEHGEQARAEVERLRGMDAERSRRQAEGEQLAATAKSAALALRDSRTGAAK
                     TIGRLVTERLVEIGFPSAAFDVHLQEAPLDESGADTVTFLLAPNPGEPPRPLARIASG
                     GEMSRVALAMKSVLAGADETPTLIFDEVDAGIGGRSADPIGRLLWNIARDHQVIVVTH
                     LAQIAVYADHHLRIEKSERDGRTLTGIEPLVGDARRSEVAQMLGGSTGTDATLAAADA
                     LLAHAAEARQTSAPVRP"
     gene            1426..2313
                     /locus_tag="VMZ33_04950"
                     /pseudo
     CDS             1426..2313
                     /locus_tag="VMZ33_04950"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
     gene            2327..3112
                     /locus_tag="VMZ33_04955"
     CDS             2327..3112
                     /locus_tag="VMZ33_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HUR16610.1"
                     /translation="MRYADTLLTEGEQVIMRTRQHWLALLSRARSGVGLWLLGILILV
                     AIVVLNVQDRTIRDVLTLVAIVFLLLGLIIFLYRLWHWWAQDYMITNRRLLKVTGILN
                     KRSGDSSLEKINDAILIQSVWGRMFNFGDLEILTAADMANDRYFMLNSPKEFKKTMLT
                     AKHQLETEFQSGRPPTPPLRAADERYEQAPPPIQAAPPPPPPAAMTAAPVMPETPSAA
                     VAPDVDESLEVTQTLARLADLRDQGAITPEEYEQKKDELLGRL"
     gene            3115..3762
                     /locus_tag="VMZ33_04960"
     CDS             3115..3762
                     /locus_tag="VMZ33_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HUR16611.1"
                     /translation="MDEALLQRLIAAAIIILISFPVHEFSHAFAAFKLGDSTARYQGR
                     LTLNPAKHFDPLGGILLLLGAVVGGFLIGWAKPTPVNPINLRGGRRSEALVALAGPLS
                     NLILAALVAIPLRIILMTPEMRGAVGSNELAVFLFGVAETFVFINLILMVFNLLPIPP
                     LDGWHVLLSLVNPRTAYTLRQYEQYGFIIIVILIVSGVTIVGDVVSTLQRWLIGS"
     gene            3759..4256
                     /locus_tag="VMZ33_04965"
     CDS             3759..4256
                     /locus_tag="VMZ33_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16612.1"
                     /translation="MNASRWWATKIRQTMRYVFGRTRPAERDALRSWLTVAQLELFDS
                     MHRADQRHGLDVVESLRRSGHAEPDLLIAGLLHDAGKGRDLHLWHRIGWSISYRHRAL
                     EPIIGRLPTFGKAFKVQRAHADVSAELAVAAGCSERTADLIRHQSEPTDLELGTALML
                     ADEAS"
     gene            4262..>4762
                     /locus_tag="VMZ33_04970"
     CDS             4262..>4762
                     /locus_tag="VMZ33_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HUR16613.1"
                     /translation="MSVMPVLISGVQIRIADARRPEDATHVRLDDFDGPIALLLSLIE
                     QRQMDVLTVRLGDLAGAYLEALARVESGRLPLLSSFITVCSQLILIKSRALLPRAPAP
                     DAAGATADAEADPEEELRRRVIEYRMYRDAGRILAERLAIPVALFHREAGVAVAAGRA
                     NARPSEE"
BASE COUNT          769 a         1607 c         1591 g          795 t
ORIGIN      
        1 ggtatcggcg gagggccgca gcgtggcgcg catcgatgat gaggccgcga ccgtgggccg
       61 gcttgcggcc gtagttgagc cactcattga gatccacggt cagcacgatc agcagcgact
      121 cctccaggga acgtggcaac gtgacgtcct tgacgcgttc ggcggtcacg cggatttgcg
      181 caccacggtg atggaggcgg tgcgagcgat gcgctccaac gaggcggccc ttcgcgagct
      241 ggtcgtagat ccggcggagc ttgagcgccg gctggagctg gctgcgttcg cggcagacga
      301 gatcgatgcc gcgtcgccgc gtgagggtga ggtcgacgag ctccgctcgg ggctcgcggt
      361 cgcgggcaac gcgcaacgca tcgccgcgct actttcggag gcccacggct tgctggccga
      421 cgaaggcagg ggagcccgtg acgctcttgc gcgggccggc cagaccacag tcgaactggc
      481 tcgactcgac ccttccgctg aagatctggc cacacgattg gacggcctgg cagccgagct
      541 cgacgaagtg gtgctcgacc tgcggcgccg cgaggacggc atcgccgagg ctgccgctga
      601 cacggagtcg gccgaggccc gactgggagt cctctacggc ctcatgcgga aatacggtga
      661 gacggaggtc gccctgatcg agcacggcga acaggcacgg gcggaggttg agcggctgcg
      721 cgggatggat gcggagcgtt ctcggcgcca ggcggaaggc gagcaactcg cggccacggc
      781 aaaatccgcc gcgctggcgc tgcgcgactc ccggacgggg gccgcgaaaa cgattggccg
      841 gttggtcaca gagcgactag tggagatcgg ctttccctcg gccgcattcg acgtgcacct
      901 ccaagaagcc ccgctcgacg agtccggcgc cgacaccgtc acgttcctgc tcgcgccgaa
      961 tcccggtgag ccgccgcggc cgctcgcgcg cattgcgtcg ggcggcgaga tgtcgcgcgt
     1021 cgcgcttgcc atgaagtcgg ttctcgccgg cgccgacgag acgccaaccc tgatcttcga
     1081 cgaggtcgac gccggcatcg ggggccgttc cgccgacccc atcgggcgac tgctgtggaa
     1141 catagcccgc gaccaccagg tcatcgtcgt cacgcacctg gcgcagattg cggtctatgc
     1201 cgatcaccac ctgcgaatcg agaagagcga gcgcgacggg cggaccctga caggcatcga
     1261 gcccctggtg ggcgacgcgc gtcggtccga ggttgcgcag atgctcggtg gctcaaccgg
     1321 caccgacgcc acgctggccg ccgctgatgc cctgcttgcc cacgcggcgg aggcgcgcca
     1381 gacctccgcg ccggtacggc cgtgacgctc ccggatgcaa tcgactcata cctgaccttc
     1441 ctgcgcgtcg agcgtggcct gtcgcccgcc accatcagcg cgtactcgac cgacctgcac
     1501 aagttcgccg ccgacgcagc caagcacggc gagcgttgga cagcggactc ggccgtcgcc
     1561 gtgaaccacc tggcgtcgct ctcgcgtccg ccgcgcgtgc tcaagccgac gacgcaccgt
     1621 cggaaggccg cagcaatccg tgcgttctat cgcttcctgt ttgctgaggg gctcatcccg
     1681 aaggacgtcg cgggcctgct ggacctgccc cgcaccagcc ggcagctgcc cgacacgctt
     1741 ggggtcgatg aggtcgaggc gctgctcgag gcgccggctt ccgacacgcc cgccgggatc
     1801 cgtgaccgcg cccttctgga gctgctctac agctgcggct tgcgtgtctc cgaggcgctc
     1861 aacctggact cggaggacgt gtccctgacc gaggccacgt tgcgcgtcat cggcaagggc
     1921 gaccgcgagc ggcgcctgcc gatcggcgag gttgccgtga tcgcggtcgc gagctacgtc
     1981 gaccacgtac ggccggagct gctgaggaag gcgcccgcga gctccatgcg ccgtggcgga
     2041 ccgttgttca tcagcgaccg cggccagcgc cttggcagaa tggccgcatg gcgcatgatc
     2101 agacgtgcgg cggtgacagg cggcgttaag ggccacgtga ccccgcacac gttgcgtcat
     2161 agtttcgcca cgcaccttct ggagggcggt gccgatctac gtgtcgtgca ggaattgctg
     2221 ggacacgcca gcatcacaac gactcagctc tacacccatc tgacgggcga acggatcagg
     2281 caggtttacg cgcgggcgca cccccgcgcc taacgcagga gggaagatgc gctacgcgga
     2341 cacgttgctc accgagggcg agcaggtaat catgcgcacg cgccagcact ggctggcact
     2401 gttgagtcga gcgcgatcag gcgtcggcct gtggctgttg ggcatcctga tcctcgttgc
     2461 catcgtcgtg ctcaacgtcc aggacaggac cattcgcgac gttcttacct tggtcgccat
     2521 cgttttcctg ctgctcgggt tgatcatctt tctctatcgg ctgtggcact ggtgggcgca
     2581 ggattacatg atcaccaacc gtcgcctgct gaaggtcacg ggcatcctga acaagcgatc
     2641 cggcgacagc tccctcgaga agatcaacga cgcgatcctg atccagagtg tctggggccg
     2701 catgttcaac ttcggcgacc tcgagatcct gacggccgcg gacatggcca atgaccggta
     2761 cttcatgctg aactccccta aggaattcaa gaagacgatg ctgaccgcga agcatcagct
     2821 cgagacagag ttccagtccg ggcggccgcc gacgccgcca ctgcgcgcag ccgatgagcg
     2881 ctacgagcaa gcaccaccgc cgattcaggc cgccccgcca ccgcctccgc cagctgcgat
     2941 gaccgccgca cctgtcatgc ccgagacccc atcggcagct gtggccccgg acgtggacga
     3001 gtcactcgag gttactcaga cgctcgcccg actggccgac ctgcgcgatc agggcgccat
     3061 cacaccggag gagtacgagc agaagaagga cgagctgctc ggccggctat agacgtggat
     3121 gaggcgctac ttcagaggct gatcgccgcc gcgatcatca tcctgatctc gttcccggtt
     3181 cacgagttca gccacgcgtt tgccgcgttc aagctcggtg actcgaccgc gcgctatcag
     3241 gggcggctga cactcaaccc ggccaagcac ttcgacccgc tcggcggcat cctgctgctg
     3301 cttggcgcag tcgtgggcgg cttcctgatc ggctgggcca agccgacccc ggtaaatccc
     3361 atcaaccttc gtggcggacg tcgcagcgag gcgctcgtgg cccttgctgg gccgctctcg
     3421 aacctgatcc ttgccgcgct cgtagcgatc ccgctgcgca tcatcctgat gacccccgaa
     3481 atgcggggcg cagttggttc aaacgagctc gcggtcttcc tgttcggcgt ggccgagact
     3541 ttcgtgttca tcaacctgat cctgatggtc ttcaacctgt tgccgatccc gccgctggac
     3601 ggctggcacg tcctcctgtc gctggtcaac ccgcgcacgg cgtacacgct gcgccagtac
     3661 gagcagtacg gcttcatcat catcgtgatc ttgatcgtgt caggcgtgac catcgtcggc
     3721 gacgttgtct cgaccctgca gcggtggctg atcgggtcgt gaacgcgtcg cgctggtggg
     3781 cgaccaagat ccggcagacg atgcgctacg tgttcgggcg aacgcgcccg gccgagcgtg
     3841 acgcactgcg cagctggctg acagtggcac agctcgagct cttcgattcg atgcaccgcg
     3901 ccgatcagcg acacgggctg gacgtcgtcg agtcattgcg gcgcagtggg cacgctgagc
     3961 cggacttgct gatcgccggg ctactccatg acgccggcaa ggggcgcgat cttcacctct
     4021 ggcatcgcat cgggtggtcg atctcttacc gccaccgagc gctggagccc atcattggcc
     4081 ggttgccgac gttcggcaag gcgttcaagg ttcagcgggc acacgccgac gtctcggccg
     4141 agctggcagt ggctgccggg tgcagcgaac gcacggctga tctgattcgc caccagtccg
     4201 agccgacgga cctggagctt ggcaccgccc tgatgctcgc cgacgaggcc agctagcggc
     4261 catgagcgtg atgccggtgc tcatctcggg agttcagatc cggattgccg acgcgcgtcg
     4321 cccggaggat gcgactcatg tgcggctcga cgacttcgac ggcccgatcg cgctgctgct
     4381 atcgcttatt gagcaacggc agatggatgt cctgaccgtg cgtctgggcg acctggccgg
     4441 cgcgtacctg gaggcgctgg cgcgtgtcga gagcggccgc ctgccactcc tgagctcgtt
     4501 catcacggtc tgctcgcagc tcatcctcat caagtcgcgt gcgttgctgc cgcgtgcgcc
     4561 ggcgccggac gcggcgggtg ccacggcgga cgccgaggcc gatcccgagg aggagctgcg
     4621 ccggcgcgtc atcgaatacc gcatgtaccg cgatgcgggt cggatcctcg ccgagcggct
     4681 cgcaattccc gtcgcattgt tccaccgcga agcgggcgtc gccgtcgctg ccggacgtgc
     4741 caacgcccgg cccagcgagg ag
//
LOCUS       DATLCO010000204         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5531751, whole genome shotgun sequence.
ACCESSION   DATLCO010000204 DATLCO010000000
VERSION     DATLCO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5531751"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..781
                     /locus_tag="VMZ33_04975"
     CDS             26..781
                     /locus_tag="VMZ33_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16614.1"
                     /translation="MNSQPLVLVADDEPRITKLVAMTLHDEGFRVVTAGGGEEALKKA
                     EEVRPDIVLLDIVMPDLDGIEVMRQLREWRPVPVILLTAKGSTSDKAKGLDLGADDYI
                     AKPFHPDELAARVRAVLRRASGVSPGAGIVAFDDIEIDLERRLLTKGGDLVSLSRTEW
                     LLLQHLAAHAGKVVLHTELLTKVWGPEYRDDLQYLRVWISRVRRKLGALPGEAGPIKT
                     FQGIGYVLDVEGNLGDSSTGGSSAAKAAAEATA"
     gene            complement(869..1288)
                     /locus_tag="VMZ33_04980"
     CDS             complement(869..1288)
                     /locus_tag="VMZ33_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16615.1"
                     /translation="MAESRSSSSKSGTSSTTQNGSSSSTSNGSKSSSGSGVGLLTNAQ
                     EAATVVKNTAEKAAEKAAERWPEVATSAQTAARDTQKALERMPNQGLLAGTSFSLGLG
                     VGLFMTGANRLMVLVAMAPAVAMAATLLARDEETAQS"
     gene            complement(1409..>2549)
                     /locus_tag="VMZ33_04985"
     CDS             complement(1409..>2549)
                     /locus_tag="VMZ33_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HUR16616.1"
                     /translation="PTIRALDRIGVETVAALASVMNISFPRGDRHLLQAGLAGAIGTV
                     ETTMVELTAGYGAMANNGVMVEPRTILEITDSDGNVISSTGQNAPRQVISQQSAWLLT
                     DILKDSTDPAVNRIFGPRLQIVNGVADPLIPGSDRRPAAAKTGTTNLLKDLSIYGYLP
                     IQSDPNLPILVASVWIGNSDNSPPNGGDVDIIAADGPGRIWSAFLREYTRDWPIAQFP
                     TPPAGVVSAEIDAWSGGSPGPWTWETRTEYFIEGTQPGGPNEVDPAGLLYRSMCGGWF
                     IDLAKVEAGKPERWLAADLDWMERARRGVGRRGPHGTTTAHLFGKQDWGGFIAPLDCS
                     LAPTPTPRPVTPPPITPPIVEPQPTDNPQTQPTPTPAPAASPAPP"
BASE COUNT          409 a          827 c          825 g          488 t
ORIGIN      
        1 ctctcgactt ccttacggag gtgcagtgaa cagtcaacca ctcgtgctcg tcgccgatga
       61 cgagcccaga atcaccaaac tcgttgccat gacccttcac gacgaagggt ttcgtgtcgt
      121 aaccgcgggg ggcggcgagg aagccctcaa gaaggccgag gaagtccggc cggacatcgt
      181 gctcctcgac atcgtcatgc ccgacctcga cggcattgag gtcatgcgcc agctccgcga
      241 gtggcgtccg gtcccggtca ttctcctgac agccaagggc tcgaccagcg acaaggcgaa
      301 gggtctcgac ctgggagccg acgactacat cgccaagcca ttccaccccg acgagcttgc
      361 ggcgcgcgtg cgtgccgtcc ttcgacgggc gagcggagtg tcgccgggcg ctggcatcgt
      421 tgccttcgac gacatcgaga tcgacctgga gcgacgtctc ctgaccaagg gtggcgacct
      481 cgtctccctc agccgcactg agtggctgct tcttcagcac ctggcggcac atgccggcaa
      541 ggtggtcctg cacacggagc tcctcaccaa ggtctggggc cccgagtacc gcgacgacct
      601 ccagtacctg cgcgtatgga tcagtcgcgt tcgtcggaag ctcggcgctc tgccgggtga
      661 ggcgggcccc atcaagacct tccagggcat cggttatgtg ctggacgtcg agggcaacct
      721 cggcgactcg agcaccggcg gaagctccgc agcaaaggca gcggccgaag ccaccgcctg
      781 acgccagacc cttcgcacac gaaaagacgg cgcgtttcac gcgccgtctt cgtgtatctg
      841 cgaccggatg ttgaccggac tttccgatct agctttgcgc cgtttcctcg tcacgcgcga
      901 gaagtgtggc agccattgcc acggctggag ccatggccac gagcaccatc aatcgattgg
      961 cgccggtcat gaacagtcca acgccgaggc cgagcgagaa gctcgtgccc gccagcaggc
     1021 cctgattggg catgcgctcg agggccttct gcgtgtcgcg tgcggccgtc tgcgcggagg
     1081 tcgctacttc aggccagcgc tcagcggcct tctcagcggc cttctcagcg gtgttcttga
     1141 cgacagttgc agcttcctgc gcgttggtca gcagccctac gccgcttccg ctgctgctct
     1201 ttgagccatt gctcgtagag ctgctgctgc cattctgagt ggttgagctt gtaccggatt
     1261 tggagcttga gctccgactt tcggccatgt ttgcctccag atcattcttc aagtgaccgg
     1321 aacatagtcg gccatggggc gtcgcagccg ctcgggggca gaggcgtgcg ctctttcagc
     1381 cttcttgcat cacgcggcta gaggctagtt acggcggtgc tgggctagcc gctggtgcgg
     1441 gagttggcgt cggctgcgtc tgtgggttat cggtcggttg gggctcgact atgggtggcg
     1501 tgatcggcgg cggagtcacc ggccgcggcg tcggggtcgg agccagggag cagtcgagcg
     1561 gcgcgataaa gccgccccag tcctgctttc cgaacaggtg agccgtcgtt gtcccgtgcg
     1621 gtccgcgccg ccctactcca cgtcgcgcgc gctccatcca gtcgaggtca gccgccagcc
     1681 aacgttccgg tttgcctgcc tcaaccttgg ccaggtcgat gaaccaaccg ccgcacatgc
     1741 ttcgatagag aaggccggct ggatcaacct catttgggcc gccgggctga gtcccttcga
     1801 tgaagtactc agtgcgtgtc tcccaagtcc aggggccagg gctaccgccc gaccacgcat
     1861 cgatctcggc tgacaccacg cccgctggcg gtgttggaaa ctgggcaatc ggccaatcgc
     1921 gcgtgtactc gcgcaggaac gccgaccaga tacggcccgg accgtcagcg gcgatgatgt
     1981 cgacgtcgcc gccgttgggc gggctgttgt cgctatttcc gatccagacc gacgcgacca
     2041 ggatgggcag gttcggatcc gattggatcg gcaggtagcc gtagatggac aggtccttga
     2101 gtaggttggt agtgcccgtc ttcgccgcgg ccggtctccg gtcgctgccc gggatcagcg
     2161 gatcggccac accattcacg atctgaagcc ggggcccgaa gattctgttc acagccgggt
     2221 ccgtgctgtc cttgaggatg tcggtcagga gccatgccga ctgctggctg atgacctggc
     2281 gcggggcgtt ctgaccggtg ctcgaaatga cgttgccgtc gctgtcggtg atctcgagaa
     2341 tcgtccgcgg ctcaaccatg acgccgttgt tcgccatcgc tccatagccg gcggtcagct
     2401 cgaccattgt tgtctcgacg gtgccgatgg cgcccgccag ccccgcctgc agcaggtgtc
     2461 gatcgccgcg cgggaagctg atgttcatca cgctggcgag agccgccacc gtctccacgc
     2521 cgatgcggtc cagcgcccga atcgtcggg
//
LOCUS       DATLCO010000205         5765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3146956, whole genome shotgun sequence.
ACCESSION   DATLCO010000205 DATLCO010000000
VERSION     DATLCO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3146956"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VMZ33_04990"
     CDS             complement(<1..473)
                     /locus_tag="VMZ33_04990"
                     /inference="COORDINATES: protein motif:HMM:NF015789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16617.1"
                     /translation="MTREATELMHPYRLGFAVKVLGAGGLKTADTRRWQSGPHLSRSL
                     EYLDAVFDYLDGIDVRVYRMSSQLIPYGTHPDLPQLDYRRQLDDARDAVERLAVKAAS
                     LGLRLSTHPGQYTVLNALDESIVAKALADIEANAVLLDVLRLPSDAAIVIHVGGG"
     gene            complement(498..1718)
                     /locus_tag="VMZ33_04995"
     CDS             complement(498..1718)
                     /locus_tag="VMZ33_04995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16618.1"
                     /translation="MDRNGRIVVFARALRSFGFGLNSVALGLYLAQLGVPGTTVGLII
                     SAAFAGSLALTVVIAGWGDRIGRRRLLMAGSALMATAALIPFVSREPLLLAVIALSGM
                     VAVNANESTGLQTVDQALLPQSVPDGQRTSAFALYNLLASAGAALGSLAVGLLPFLGA
                     ALGLTGAHIFVPAFAVYAAIGLITLALHARLDARAESGERLEGRLAIDKSRGVVARLS
                     ALFGLDSFASSLSVQSYIAYFLVARFAFEPQTAGVLFFVAGVLTTLSFPVAAWLSTRI
                     GLIHTMVFTHIPSSVFLLGLALATDVWVAAAFLLGRAALASMDVPARQSYTMAVVEPR
                     ERTATAGVTSLARAIAQVPGPAIAGALLVPIGLGVPLMATAALKITYDLLLFRLFKSR
                     PAPEEPARQRDASA"
     gene            1872..3293
                     /locus_tag="VMZ33_05000"
     CDS             1872..3293
                     /locus_tag="VMZ33_05000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUR16619.1"
                     /translation="MRIDQLALLFALVIMANIVVLVALEVPSFRRLRRTTTREADHMI
                     EMGTPSATAQFGETGPTPMEAGLYQRVVRVVSFLFIGSVLVIETLAGGQRIGVYVVCA
                     VGVFAIVLLQDVLPAGRLGRWRLPLEAGSAVAFLTMLLILTGGQESAYFFGYILLLGA
                     GSLWASGLGPVVLALVTSVSYLFAVVIASGRALENPEAIGRVAFNLVALALVTYIAAV
                     IGREQRRAREEALRLSRFDPMTTLHSRSYYESAVEQEILRAARTGRGFALVMVDLDGL
                     KAANDRFGHDSGDRLLKAVAEVMRGDIRATDVAARYGGDEFVMMLPETDLQGALRVAD
                     KVRVDISRLALPHNGSLIRTSASIGVVTFPDDGRTSVELMRRADLAMYEAKRRGRDQV
                     VRYAREHGVPVESEVHGGPRGQAEWTHRTDLPPPRRDGTPPQPTGSPGYPPRTTAPAG
                     VRAAPSSIDVPPESPFPAPWERR"
     gene            3338..4558
                     /locus_tag="VMZ33_05005"
     CDS             3338..4558
                     /locus_tag="VMZ33_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUR16620.1"
                     /translation="MTEPRSRRDAGFGTRAIEAATRAPEIHQEPGMVPIYQTVTFNAH
                     DSTELGDILADRQPGYAYSRIDNPTSQAVGDAVAEMHGAPAGFAFASGMAAAHAMFVS
                     LLQAGDHVIAANAIYGSVHKLLDKVLTRLGIEVSFVDITDVDALRAAFRPNTRVVHVE
                     SIANPNLIVTDTQAVAEIAHRHGAVLTVDNTFASPYLCRPLELGADLVMDSCTKWISG
                     HSDVLGGTVVGSPELIRSVRSVQVDTGGSLAPFSAFLVLRGITTLHVRMERHSSNALA
                     LARALEGRDIVRQVLYPGLPSHPQFNVAQRVLRAGGGMLAFDLGDRLAAGAFIDALTV
                     PVRTASLGSVMTIAVHPPSTTHRQLDEAALAAAGIREGLVRVSVGLEDIDDLLTDFEH
                     GLNAARTTATTRSA"
     gene            4570..>5765
                     /locus_tag="VMZ33_05010"
     CDS             4570..>5765
                     /locus_tag="VMZ33_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HUR16621.1"
                     /translation="MPGAGEQHRTLHGNTDIATLALPGRRTASLNPLARLGRAAWRTL
                     TSVKFAVLQISVLSVAGVVGTILDQIPAFALHDPAAYAGQLATLHAKYDGAAILGVHL
                     GPGMVDLFDKLGFFRVFSAPWFILLLTLLVVAIVVCTLDRTPDLWRKARLVKIKQAAP
                     FFDLRLSERARFTNADDAAADELRKVLRAKHYNVREEIEQVAAADTHAGAGATATPTR
                     YVYGDRNQYMKLTTLFTHLGLILFLAGGAVTTAMGYETVVFLGQGETAPVQAVGTQGN
                     LLVKNIFFEAPQDGAGNFTDFRTDLAVYRDGQEVARKIIRVNDPLEVGGFVFHQNTFG
                     PAEDLIIRDPDGRLVWDGPVLLTDALGGRPQGFMTVPGSDIGLLLVLDKTADGMGLLV
                     MTGVKRT"
BASE COUNT          990 a         1907 c         1900 g          968 t
ORIGIN      
        1 tagccacccc cgacgtgaat gacgatggcc gcgtccgacg gcagtcgaag gacgtcgagc
       61 aggacggcgt tggcctcgat gtcggcaagt gccttggcca cgatcgactc gtcgagggca
      121 ttcaggacgg tgtactggcc cggatgcgtc gagaggcgga gacccagcga cgccgccttg
      181 acggcgagtc gctccacagc gtcgcgagca tcgtcgagct ggcgccggta atcgagctgc
      241 ggcagatcgg ggtgagtgcc gtacggaata agttggctcg acattcggta gacgcgcacg
      301 tcgatgccgt cgaggtagtc gaagacggcg tccagatatt caagcgagcg tgacaggtgc
      361 ggacctgact gccagcgccg cgtgtcggcg gtcttcaacc caccggcgcc gaggaccttg
      421 acagcgaagc cgagtcgata tggatgcatc aactcagtag cctcgcgggt cacctggaat
      481 cccgctctgg cggagagcta ggccgacgcg tcccgttggc gcgccggttc ttcaggggct
      541 ggccgcgact tgaagagtct gaacagcagg aggtcgtagg tgatcttcaa cgcagcggtc
      601 gccatgagcg gcacgcccag gccgatcggt acgaggagcg cgccagcaat ggcgggtccg
      661 ggcacctggg caatggccct ggccaagctg gtcacgccgg cagtcgcggt tcgctcgcgc
      721 ggctcaacca cggccatggt gtacgactgg cgcgctggga cgtccattga tgccagggcc
      781 gcccggccga gcagaaaggc agcggcgacc cagacgtccg tggccagcgc cagcccgagc
      841 aggaagacgc ttgacggaat gtgcgtgaag accatcgtat ggatgagccc gattcgagtt
      901 gacaaccatg ctgcgacagg gaacgacagc gtggtcagca caccggcgac gaagaacagc
      961 acacccgcgg tctgcggctc gaaagcgaat cgggcaacca ggaagtaggc gatgtagctc
     1021 tgcacggaca ggctgctcgc gaacgagtcg agcccgaaca gcgccgatag gcgcgccacg
     1081 acgccgcgcg acttgtcgat tgccagccgc ccctcgaggc gctcgccgga ctcggcgcgg
     1141 gcatcaagcc gtgcatggag agcgagcgtg atcaggccga tcgcggcgta gacggcgaac
     1201 gccggcacga agatgtgcgc gcctgtcagg cccagcgcag cgccgaggaa aggcaaaagg
     1261 ccaactgcca atgagcccag cgctgccccg gccgaagcaa gcaggttgta gagcgcgaac
     1321 gcgctggttc gctggccgtc ggggaccgac tgcggcagca gcgcctgatc gaccgtctgc
     1381 agtccagtcg actcattcgc attgacggca accatgcctg acagcgcgat caccgccagc
     1441 agcaacggct cgcggctgac aaacgggatg agcgcggccg tggccatgag cgccgagccc
     1501 gccatgagca gccgtcgccg tccgatgcgg tccccccagc cagcgataac gacggtcaac
     1561 gcgagcgatc cggcgaaggc cgcggagatg atcagcccga cggttgtacc gggaaccccg
     1621 agctgcgcca ggtacagacc gagggcgacc gagttgagcc cgaacccaaa gctgcgcagc
     1681 gcccgggcga agaccacgat gcggccgttt cggtccaaat gcggcaatcg gccgtcgtgc
     1741 ctggaatcag gttgtggatg ctgttgcatg gtgtccacgc taattaagca gtgcgtactt
     1801 ctggatgcac gaccgcatta ccatcgggtc aggtcctcgc agttgtgcaa ccaggtagcc
     1861 acgaatccaa ggtgcggatc gatcagctcg ccctcctgtt cgccctcgtc atcatggcca
     1921 atatcgtcgt tttggtggcg ctcgaagttc ccagctttcg gcgcctccgt cgaacgacga
     1981 cgagggaagc cgaccacatg atcgagatgg gcacaccgag tgcaaccgct cagttcggcg
     2041 aaacaggtcc gactccaatg gaggccgggc tctaccagcg tgtcgtgcgc gtggtcagct
     2101 tcctgttcat cgggtccgtg ctggttatcg agaccctcgc cggcgggcag cggataggcg
     2161 tttacgtcgt ttgcgccgtt ggcgtctttg cgatcgtcct cctgcaggac gtgctcccgg
     2221 cgggacggct cggacgctgg cggctaccgc tcgaagctgg tagcgcggtg gccttcctga
     2281 ccatgctgct gatccttacg gggggccagg agagcgccta tttcttcggc tacatcctgt
     2341 tgctgggcgc ggggtccctt tgggcatccg gccttggccc agtcgtactg gcgctcgtca
     2401 ccagcgtgtc gtacctgttc gccgtcgtga ttgcgtcagg cagggcgctc gagaacccgg
     2461 aggccatcgg ccgggtggcg ttcaacctcg tcgcgctggc attggtcacc tatatcgcag
     2521 cggtgattgg ccgcgaacag cgccgcgcgc gggaggaggc attgcgactg tcgcgtttcg
     2581 acccgatgac gacactccac agccgcagct actacgaatc ggcggtcgag caggagattc
     2641 tgcgcgccgc acgcacgggt cgcggattcg cgctggtgat ggtcgacctt gacggcctca
     2701 aggcagccaa cgaccggttc ggccacgact ccggtgaccg tttgttgaag gcggtagcgg
     2761 aggttatgcg cggcgacatc cgggccacgg acgtcgccgc gcgctacggc ggggatgagt
     2821 tcgtgatgat gctcccggag acggacctgc aaggggcgct gcgcgtcgcc gacaaggttc
     2881 gcgtcgacat ctctcgtctc gcgctgccgc acaacgggtc gctgatacgc acgtcggcgt
     2941 caatcggtgt ggtgactttc cccgacgacg ggcgcacctc cgtcgagctg atgcggcgcg
     3001 ccgacctcgc catgtacgag gccaagcgcc gcggacgcga ccaggttgtc cgttacgccc
     3061 gtgaacatgg agtgccggtc gagtcggagg tccacggcgg gccccgcggg caggccgagt
     3121 ggacgcatcg gaccgacctc ccgccgccgc gtcgcgacgg cacgccgcca cagccaaccg
     3181 gttcgccggg ttacccgcca cgaacgacgg cgccggctgg cgttcgcgcg gccccaagct
     3241 ctatagacgt cccacccgag tcgccgtttc ccgcgccatg ggagcggcgc tagcccctgt
     3301 gcggaggtaa accgctccgg tcacctaaca tttcggcatg accgaaccac ggtcccggag
     3361 agatgccggg tttggcacgc gcgccatcga ggcggcgacc cgcgcccccg aaatccatca
     3421 ggagccgggc atggtcccga tctaccagac ggtgaccttc aacgcccacg actcgaccga
     3481 gctgggcgac atcctcgctg accggcagcc cggctacgcg tactcgcgca tcgataaccc
     3541 gacctcgcag gctgtcggcg atgccgtcgc cgagatgcac ggcgctccgg ccggcttcgc
     3601 ctttgcgtcg ggcatggccg cggcgcacgc gatgttcgtg tcgctgctcc aggcgggtga
     3661 ccacgtcatc gccgcaaacg ccatctacgg cagcgtgcac aaactcctcg acaaagtgtt
     3721 gacacgcctc ggcatcgagg tgtcgtttgt cgacatcacg gacgtcgacg cgctcagggc
     3781 tgcattccga ccaaacaccc gggtggtcca cgtcgagtcg atcgccaatc cgaacctcat
     3841 cgtcaccgac acccaggcgg ttgccgaaat cgctcaccgc cacggagcag tcctgaccgt
     3901 cgacaacacg ttcgcctcgc cgtacctgtg tcggccgctg gagctgggcg cggacttggt
     3961 gatggactcg tgcaccaagt ggatcagcgg ccactctgat gtgctcggag ggaccgtcgt
     4021 gggctcgccc gagttgatca ggtctgtgcg ctcggtccag gtcgataccg gcggctcgct
     4081 ggcgcccttc agcgccttcc tggtgctccg gggcataacg acgctgcacg ttcgtatgga
     4141 gcgccactct tctaacgcgc tggcgctagc gcgcgccctg gagggccgcg acatcgtccg
     4201 ccaggtgctc tacccaggcc tgcccagcca tccgcagttc aacgtcgcgc agcgcgtcct
     4261 gcgcgctggc ggtgggatgc tcgccttcga tctcggggat cgcttggccg ccggggcctt
     4321 catcgatgcc ttgaccgtgc ctgtacgcac ggcgtcgctg ggcagcgtca tgacgatcgc
     4381 cgtccacccg ccgtcgacga ctcatcgtca gctcgacgag gcagcactcg cagctgcagg
     4441 gatccgcgag ggcctcgtgc gcgtctctgt agggctcgaa gacatcgacg acctgttgac
     4501 cgacttcgag cacggcctga acgccgcacg caccacggcc acaacccggt cggcctgaca
     4561 gccgcgcctg tgccaggcgc aggcgagcag catcgaacac tccacgggaa cactgacatc
     4621 gccacactcg cccttcccgg ccgtcgcacc gcgagcctga atccgctggc ccggcttggc
     4681 cgtgccgcgt ggcggacgct taccagcgtc aagttcgccg tacttcagat ctccgtcctg
     4741 tccgtggcgg gcgtcgtcgg cacgatcctc gatcagatcc cagcctttgc cctacacgac
     4801 ccggcggctt acgccgggca gctcgcgacg ctccatgcaa agtacgacgg ggccgcgatt
     4861 ctcggcgtcc acctcggtcc gggcatggtt gatctcttcg acaagctcgg cttcttccga
     4921 gtcttctcag cgccgtggtt catccttctg ctgaccctgc tggtcgtcgc catcgtggtc
     4981 tgcacgctcg atcggacgcc cgatctctgg cgcaaggccc gcctcgtcaa gatcaagcag
     5041 gcggcgccat ttttcgacct ccgcctgagc gagcgcgccc gatttaccaa cgccgacgac
     5101 gcggcggctg acgagctgcg gaaggtgctg cgcgccaagc actacaacgt tcgcgaggag
     5161 atcgaacagg tcgcggcggc cgatacacac gcgggtgccg gcgccacagc gaccccaact
     5221 cggtacgtct acggtgaccg caaccagtac atgaagctga ccactctctt cacgcatctc
     5281 ggcctgatcc tgttccttgc cggcggtgcg gtgacgactg cgatgggtta cgagacggtc
     5341 gtcttcctcg ggcagggcga gactgcgcct gtgcaggccg tggggacgca gggaaacctg
     5401 ctcgtgaaga acatcttctt cgaggcgccg caagatgggg cggggaactt cacggatttt
     5461 cgtaccgacc tggccgttta tcgcgacggc caggaagtgg cgcgcaagat catccgggtc
     5521 aatgacccgc ttgaggtcgg cggcttcgtc ttccatcaga acacatttgg ccccgcagag
     5581 gacctgatca ttcgggatcc ggatggtcgg ctcgtctggg atggcccggt gttgctgacg
     5641 gatgcgctgg gcggacggcc gcagggcttc atgacagtgc ctggttcgga catcggcctt
     5701 cttcttgtac tcgacaagac ggcggatggc atgggcctgt tggtaatgac gggcgtcaag
     5761 cggac
//
LOCUS       DATLCO010000206         4461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_313996, whole genome shotgun sequence.
ACCESSION   DATLCO010000206 DATLCO010000000
VERSION     DATLCO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313996"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..512)
                     /locus_tag="VMZ33_05015"
     CDS             complement(96..512)
                     /locus_tag="VMZ33_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16622.1"
                     /translation="MSGSVGVGVGVGVGVGSTGASVGLGVGVGVGVGVGVGVGVGVGV
                     GVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGV
                     GVGGGVAPGVSSKIGELSNVRCAALVVASVPLFSNS"
     gene            851..2230
                     /locus_tag="VMZ33_05020"
     CDS             851..2230
                     /locus_tag="VMZ33_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16623.1"
                     /translation="MDQTVLRGRISALAVLAFSLVLAVGASPASAAPGATSDALRYAG
                     MPVPDALSEPTASEPTFSLGAATIRLRGYQTLFRIDREGRIGTRTHVQFGTGWVYLAR
                     QEPKTFGTGRFVQLLSGPYTSWWVSAPDASLEAPAPFPAGSKVRLAAGTYLGVKFYAS
                     GSVRIRRPLTLQAAAEFETTRHATFAGREYYQLSSGPLAGRWVAGYRASLVGQAGSPP
                     PPEPAVPEPEATWKALALVYRETDVTFTRADGTDYHLQARMSAEMHGLVLDTLRRTKS
                     SVTGWSSGLAAMQLTVVDVPHPLRALDKLSNSYWVGPRSVEADINEYAPPGAYDSIFV
                     VWESKDAAGEKVPVGGWGLSLPPGSWANGAGYSSVTTPTQMWWWTSSEAPEEVFIHEW
                     LHQVLYFHRDAGRMDFDLHAGAQYGYEPDNGSWKRWLADVMRGLVRDGNRYVGITPEL
                     WRAGSPTQP"
     gene            complement(2227..3180)
                     /locus_tag="VMZ33_05025"
     CDS             complement(2227..3180)
                     /locus_tag="VMZ33_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HUR16624.1"
                     /translation="MRYALMTEPQQGLSYDDIAAVATTAERVGFEAFFRSDHYGSFPG
                     DRGMPTTDAWATLGGLARETSTINLGVLVSPVTFRSPGNMAKVVATVAEMAGGRVELG
                     LGAGWNEPEHREHGLPFPEIKQRFDMLEEQLAIVHGLWTEPDGWSYAGAHWTVDEALF
                     HPKPPASGDRRHPNLILGGAGGPRLTRLAATYADELNISSADPAMVGAAYGRLADACR
                     AIGREPGDVTRSAMTGCLIANTEERLRARVRELLVVIGGRDDPNEWLAERQHRWVIGT
                     PDQARRRVDEFAAAGVERLMLQDFLPRDLSMVEEAAEIFLG"
     gene            complement(3185..4114)
                     /locus_tag="VMZ33_05030"
     CDS             complement(3185..4114)
                     /locus_tag="VMZ33_05030"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HUR16625.1"
                     /translation="MNRQSIPAWIPALSLIALLTLGCSGAASLPYDDQGNQRAPVAAQ
                     PASADRQDGAAVGEEAPSGSDTNSDGEQQVADGALIIHTGTLDLEVTELRPAVDQATS
                     LIRGLGGNVAESHESNSDGRHTATVSYRIPAARWDEAVAALRAIGQRVVAEDIEAQDV
                     TSQVIDLDARIANLRTTEAALQAIMVEAKTITDVLKVQSELTSVRSDIESMTAQRDHL
                     ADQAALGTLEVRFGGPVMATTAATQGWDLGHEVDAALAALVKLGQGVASLGVWALIVF
                     LPVAIPIVGIFYLALWLRRRYAARHRVTTALSE"
     gene            4222..>4461
                     /locus_tag="VMZ33_05035"
     CDS             4222..>4461
                     /locus_tag="VMZ33_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR16626.1"
                     /translation="MDILPIPDGLVADGTLGRPFEPVCALDELALGDMTRFSRGDRDF
                     LLAHTPLGLCLTDDRCPHMAAPLSIGTLTNCVVACP"
BASE COUNT          751 a         1544 c         1430 g          736 t
ORIGIN      
        1 atagactggc tgcggatgct tgacggggcc gtcgcgacga acgcctcgac gcctccggcc
       61 gacgccccgc ccgcgggcgc gggccagctc tggcgctacg agttcgagaa caacggcacc
      121 gacgcgacga ccagcgccgc gcacctgacg ttcgacaact cgccgatctt cgaggacacc
      181 ccgggcgcga cgccgccgcc gaccccgaca cccaccccga cgcccacgcc gacgcccacg
      241 ccgacaccca cgccgacgcc cacgccgaca cccacgccga cacccacgcc gacacccacg
      301 cccacgccca cgccgacacc gacaccgacg ccgacgccga cgccgacacc gacgccgacg
      361 ccgacgccga cgccgacgcc aacgcctaca ccgacgccga cgcctacacc tacaccgacg
      421 ccgacgccaa cgccaacgcc cagccccacg gacgcacccg tggatccgac gccgacaccc
      481 acgccgacgc cgacgccaac acttccggac acgggaattg cgtcgccgac cgacgcgaca
      541 ccgcccgatc acagcaacgt gatgctggtc gtgctcttga gcacgatggt cggtttctct
      601 gtccttgtgc ggccgcttcg gcgacgcgtg gaggtgcggg actaacccga tagcgagcta
      661 gatcgcacca aaacaggggg tcaggggcgc agttagctgc ggtctggtgg ggtgagtgcc
      721 agacctttgg ccacaaatct gcctctttgg ggtgataccc caaccatgac cggcctccga
      781 tgctcgggcc gcggccagtt gccgtggaac ccgacgggca attgccccgt cgcccgccct
      841 cggaggcaag atggatcaga cggtcctgcg tggacgaatc tcggcgcttg ccgttctcgc
      901 tttctcgctc gtcctggctg ttggtgccag tccggcgagc gccgcgcctg gggctacgtc
      961 agacgccctg cgctacgccg gcatgccggt gccggatgca ttgagcgagc caaccgcatc
     1021 ggagccgacg ttctcgcttg gcgccgcaac aatccgcctc cgcggatacc agaccctctt
     1081 ccgcatcgac cgcgaagggc ggatcggtac ccggacgcac gttcagtttg gtaccggatg
     1141 ggtctatctc gctcggcaag agccgaaaac gttcgggact ggccgattcg tgcagctact
     1201 gagcgggccg tacacgtcgt ggtgggtatc tgcccctgac gcgtccctcg aggcgccggc
     1261 cccctttcct gcgggttcca aagttcgtct agcagccggg acgtatctgg gcgtcaagtt
     1321 ctacgcctct ggctcggtca gaatccgacg gccgctgacg cttcaggcgg cggccgaatt
     1381 cgagacaact cggcacgcga ccttcgctgg tcgcgagtat taccagctct cgagcgggcc
     1441 actcgccggg cgctgggtcg ctggttaccg ggcctctttg gtcggccaag cgggttcgcc
     1501 gccgccacca gagcccgctg tcccggagcc ggaggcgacg tggaaggcgc tcgcccttgt
     1561 atatcgggaa accgacgtga cgtttacccg tgccgatggc accgactacc acctccaggc
     1621 gaggatgagc gccgagatgc acgggctcgt cttggacacg ctgcgtcgaa cgaaatcatc
     1681 ggtgacaggc tggtcaagtg gtctcgccgc tatgcaactg accgtcgtgg acgtaccgca
     1741 ccccctgcgc gcgctcgaca agctgtcgaa ttcctattgg gttggcccgc gtagcgtgga
     1801 agccgacatc aatgaatacg caccgccggg cgcttacgac agcatcttcg tggtttggga
     1861 gtccaaggat gcggcgggcg agaaggtgcc tgttggcggc tggggcttgt cgctgcctcc
     1921 gggatcgtgg gcgaatggtg cgggttactc gtcagtaact acacctacgc agatgtggtg
     1981 gtggaccagc tcagaggcac ccgaagaagt tttcattcat gaatggcttc accaggtgct
     2041 ctactttcac cgtgacgcgg gcaggatgga cttcgatctg catgcgggcg cccaatatgg
     2101 ctatgagccc gacaacggct catggaagcg ctggctggcg gacgtaatgc gcggactggt
     2161 ccgcgacggt aatcgctatg tcggcataac gcccgagctt tggcgagcgg ggagtccgac
     2221 ccagccctag cccaggaaga tctccgcggc ttcctcgacc atcgagaggt cgcgcggcag
     2281 aaagtcctga agcatcaggc gctcgactcc agccgcggca aactcgtcga cgcgccggcg
     2341 ggcctggtcg ggagtgccga tcacccaccg gtgttgccgc tcggcaagcc actcgttggg
     2401 atcgtcccga ccaccgatta caaccagcag ctcccgcacc cgcgcgcgca gccgctcctc
     2461 cgtgttcgcg atcaggcagc cggtcatcgc cgagcgcgtg acgtcacctg gctcccgacc
     2521 aatggcacgg cacgcgtcgg ccagccggcc gtaggcggcg ccgaccatgg cggggtcggc
     2581 cgagctgatg ttcagctcat cggcgtaggt cgccgccagt cgagtgaggc gcgggccgcc
     2641 agcgccaccc aagatgaggt tcgggtgccg gcgatcccct gatgccggcg gcttggggtg
     2701 gaacagcgcc tcgtctacgg tccagtgtgc gcccgcatac gaccaaccgt ctggctctgt
     2761 ccaaaggccg tgcacgatgg cgagttgctc ctcgagcatg tcgaagcgct gcttgatctc
     2821 cggaaagggc aggccgtgct cccgatgctc gggctcgttc cagccggcgc cgaggcccag
     2881 ctcaacgcgc ccaccggcca tctcggcgac cgtcgcaacg acctttgcca tgttgccggg
     2941 ggagcggaag gtaaccggcg agacgagaac accgagattg atcgtgctcg tctcccgcgc
     3001 caggccaccg agcgtggccc acgcatcggt ggtcggcatc ccgcggtcgc ccgggaagct
     3061 gccgtaatga tcggagcgaa agaaggcctc gaagccaact cgttcagcgg tcgtggcaac
     3121 ggccgctatg tcgtcgtagg acaagccctg ctggggctcc gtcatcaggg cgtagcgcat
     3181 cgcctcattc tgagagtgct gtggtaactc ggtggcgcgc cgcgtaacga cggcgcagcc
     3241 acagcgccaa atagaagatg ccgacgatcg gaattgccac cggcaggaag acgatcaacg
     3301 cccagacgcc gaggctggct acgccctggc cgagctttac gagcgctgcc agcgcagcat
     3361 cgacctcatg tcccaggtcc cagccctgcg tcgcggctgt ggtggccatg accggtccac
     3421 cgaacctgac ctctagcgtg ccaagggccg cttgatcggc aagatggtcc cgctgcgcgg
     3481 tcatgctttc gatgtcagag cggacgctcg tgagctcact ctgaaccttg agcacgtcgg
     3541 tgatggtctt ggcctcaacc atgattgcct ggagcgcggc ctcagtagta cgcaggttgg
     3601 cgatgcgcgc gtccaaatcg atgacctggc tcgtgacgtc ctgcgcctcg atgtcctcag
     3661 cgaccacacg ctggccgatg gcacgcagcg ctgcgacggc ctcatcccag cgggcggcgg
     3721 ggattcgata agagacggtg gccgtgtggc gaccgtcgga gttggactcg tgcgactcag
     3781 cgacgttgcc acccaagccg cggatgagtg acgtcgcctg gtccacggcc ggccgaagtt
     3841 cggtgacctc gagatcgagc gtgccggtgt ggatgatgag cgcgccgtca gcgacctgct
     3901 gctctccatc gctgttcgtg tcagagcctg agggggcttc ctcgcccacg gctgcgccat
     3961 cttgtcggtc cgcactggcg ggctgggccg cgaccggggc gcgctgattg ccctggtcgt
     4021 cgtatggcag gctggccgct ccggagcagc ccagggtgag cagcgcaatc agtgacagag
     4081 ctggtatcca cgctgggatc gactgtcggt tcatggttgt cctccgtgcc cccaacgggc
     4141 gaagcatttc acctacatgt tgggacgcgg ccaccacacg attaagttcc gcgaaccgtg
     4201 caaccatccc gtgcgatgga cgtggacatc cttccgattc cagatggcct cgtggccgac
     4261 ggcacccttg gccgcccctt cgagccggtc tgcgcgctag acgagctggc ccttggcgac
     4321 atgactcgtt tctcgcgcgg cgaccgcgac ttcttgctgg cccacacgcc gctcggcctg
     4381 tgcctgaccg acgatcgctg cccgcacatg gccgcgccgc tgtcgatcgg caccctgacc
     4441 aactgcgtcg tggcgtgccc g
//
LOCUS       DATLCO010000207         4274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2550237, whole genome shotgun sequence.
ACCESSION   DATLCO010000207 DATLCO010000000
VERSION     DATLCO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550237"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VMZ33_05040"
     CDS             complement(<1..1069)
                     /locus_tag="VMZ33_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16627.1"
                     /translation="MLTGLLAVAAGACSSAPRTTPAGSVGQPTAAPTTAALTEEVVSV
                     GAALALMRGHHIAARELFASGDASGALAHATHPVAEILDSVRGDIDAKNGDAAALGVA
                     LQLVISAAQGDSEAALGTAIDGAAVVMDAAEQAVAGSLVSDTAYAGSVIAATLDTAGH
                     EYAEAVTESGIGELIEYQDAYAFTRQAAADYAEIAAPIGAGHPAEAAEIDEAFEVLGV
                     ALPGVQPPATIAAVEDVEAAAELIAHELEEVVGALPVSESDPAAEQAAIEGLLDEISE
                     LVAGGDRVQAAEVAAEAYLDHYEVIEAAVIAAAPDINDELEPLLGAGLRQQINAGATV
                     EEIDGTIARVKALLGQAIEALE"
     gene            1248..1991
                     /locus_tag="VMZ33_05045"
     CDS             1248..1991
                     /locus_tag="VMZ33_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HUR16628.1"
                     /translation="MPPPDHGEHELSAASQAYLLTARSMTASGQAVTVSAIAARMQVS
                     KQAASEMVARLSREGLVEPGSDRDLRMTGEGRDAADAIFRRHALLEWLLTRVIGLGWA
                     ESDEEAMRLQGAVSPRVESAIRDLVGDPKTCPHGNPIDLATALERPAGVALSDAEPGS
                     EITIYRITEEAEEDADLLSYLEQQRLVPGIVARVVEVSHSRDSIVLDGPTGRASMGLR
                     PASLIRVLPGRADSSLFHQMPDRSAPRPE"
     gene            2235..2648
                     /gene="rpsL"
                     /locus_tag="VMZ33_05050"
     CDS             2235..2648
                     /gene="rpsL"
                     /locus_tag="VMZ33_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HUR16629.1"
                     /translation="MPTINQLVRKGRVAKVKKVKAPAMRKNWNSLRQRQESIPGAPQK
                     RGVCLQVRTMTPKKPNSALRKIARVRLSNQQEVTAYIPGIGHNLQEHSVVLVRGGRVR
                     DLPSVKYHIIRGTLDAAGVRDRKQGRSLYGAKRPK"
     gene            2648..3112
                     /gene="rpsG"
                     /locus_tag="VMZ33_05055"
     CDS             2648..3112
                     /gene="rpsG"
                     /locus_tag="VMZ33_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HUR16630.1"
                     /translation="MPRRAQSPRKDKYAPTLTRTTDRFVVKLMMDGKKGLAERILRQA
                     LARAEAATRRPGLEVLDAALRNATPIIEVKPRRVGGATYQVPVEIRGDRRVSLGVRWL
                     VQAARKRSGKSMSEKLAAEFVDAMNGVGAAVKRREDTHKMAEANKAFSHFKW"
     gene            3121..>4274
                     /locus_tag="VMZ33_05060"
     CDS             3121..>4274
                     /locus_tag="VMZ33_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUR16631.1"
                     /translation="MTSPRDTANSASRQVPLARTRNIGIIAHIDAGKTTVTERILFYT
                     KKIYKIGEVHEGAATMDWMPQEQERGITITAAATTAFWGDHRINIIDTPGHVDFTVEV
                     ERSLRVLDGAVVVFDGVAGVEPQSETVWRQADRYGVPRFCFINKLDRTGADFWRCVQS
                     IEDRLGANAVPIQIPIGREDKFQGVIDLVEMKAVYYRDDLGNQIDVVDIPAELMDEAQ
                     KHHHKMIEAVAEMDDDLTHKYLEGQDLTTDEIRRGLRLGTLSTKIIPVLTGSALKNKG
                     VQKMLDAVVDYLPSPLDVPPMVATDPDTGAESVRPPSDDAPFSALAFKIAADPFVGKL
                     AFFRVYSGTLKSGSYVLNPVKGKRERIGRILQMHANHREEIEEVFAGDIAA"
BASE COUNT          750 a         1437 c         1409 g          678 t
ORIGIN      
        1 gctcgagagc ttcgatggcc tggccgagca gagccttgac gcgagcgatg gtcccatcga
       61 tctcctcgac cgtcgcgccc gcgttgatct gctgacggag tccggccccc agaagcggct
      121 cgagctcgtc gttgatgtca ggtgcagcgg cgatcactgc ggcctcgatg acctcgtagt
      181 ggtcgaggta tgcctctgcg gccacctcgg ctgcctggac gcgatcgccg ccagcgacga
      241 gctcgctgat ctcgtcgagc aggccctcga tggccgcctg ctccgcggct gggtcggatt
      301 cgctgaccgg cagcgcccca acgacctcct cgagctcgtg tgcgatgagc tcggcggcgg
      361 cctcgacgtc ttcgaccgcg gcgatcgttg ccggcggctg gacaccgggc agggcgacgc
      421 cgaggacctc gaacgcttcg tcgatctcgg ccgcttccgc tgggtgaccc gccccgatcg
      481 gcgcggcaat ctccgcgtag tcggccgcgg cctggcgcgt gaatgcgtac gcatcctggt
      541 actcgatcag ctcgccgatg ccggactccg tcacggcttc tgcatattca tgcccggcgg
      601 tgtcaagcgt cgccgcgatg accgagcctg catacgcggt atctgagacg aggctgccgg
      661 cgaccgcctg ctccgcggcg tccatgacca cagctgcacc gtcgatcgcc gtgcccagcg
      721 ccgcctcgga gtcgccctgg gcagcgctga tgaccagctg gagcgccact cccagggcag
      781 ccgcatcgcc gttcttcgcg tcgatgtcgc cacggacgga gtccaggatc tctgccactg
      841 gatgtgtcgc gtgcgccagc gcgccgctgg cgtcgcctga cgcgaagagc tcacgcgcgg
      901 cgatatggtg gccgcgcatc agcgccagcg cggcgccgac cgagacgact tcctcagtga
      961 gcgccgccgt ggtcggagcg gccgtcggtt ggccgactga gccggcgggt gtcgtgcggg
     1021 gcgcactgct acatgcgcca gcggccacgg ccaggaggcc ggtcagcacg aggaggcgag
     1081 cggttgacga ggtgagtacc aaagatgtct ccggcagatt gttcggcgct attccgactg
     1141 gtggtgtcgg atatagggaa tggtaggaac gaccattcga cccgtcaact gcccatgcat
     1201 gtcgccgtca ggtgaccttg acggcaggaa cggactacga tcgaccggtg ccaccacccg
     1261 accacggcga acacgagttg agcgctgcca gccaggcgta cctgctgacg gcccgctcga
     1321 tgactgccag cggccaggct gtgacggtat cggcaatagc ggcgcggatg caggtttcga
     1381 agcaggccgc gtcggagatg gtggcccgcc tgtcgcgcga gggactggtc gaaccggggt
     1441 ccgatcgcga ccttcgtatg acgggggagg gacgggacgc cgccgatgcc atcttccgcc
     1501 ggcatgccct gcttgaatgg ctgctgaccc gggtaatcgg tctcggctgg gctgagtcgg
     1561 atgaggaggc gatgcgcctt cagggcgccg tatcaccgcg tgtggagtcg gcaatacgcg
     1621 accttgtcgg cgaccccaag acatgcccgc atggcaaccc gatcgacctc gccacggccc
     1681 tggagcgccc ggcaggtgtg gcgttatccg acgccgagcc cggctcggag atcaccatct
     1741 accgcatcac cgaggaggcg gaggaggacg ctgacctgct ctcgtacctg gagcagcagc
     1801 ggttggtgcc cgggatcgtg gcgcgcgtcg tggaggtgtc gcacagccgc gactcgatcg
     1861 tcctcgacgg tccgacaggt cgcgccagca tgggccttcg gccggcgtca ctgatacggg
     1921 ttctgcccgg ccgcgcagac tcgtcgctgt tccaccagat gccggatcgg tccgcgccgc
     1981 ggcccgagta gcgccgctgc cgcggtcgtt gcctgatctg gccgatcttg gtacactgcg
     2041 cgatcgtggc ctgaggtcac acataccgat cccgacgcgg gagagggcac cgcgggtaat
     2101 ttccaagtcg ccaaggcagg tcttggcggt gccgcccgag aggctctgcg tcgggtcaat
     2161 ccaccaaagc caatagtccg tcggtaactg cgcgccgcca ggcccaggct gacagccaac
     2221 aggagttctc gttcgtgccg acgatcaacc agctcgtgcg caagggccgc gtggccaagg
     2281 tgaagaaggt caaggcgcca gctatgcgca agaactggaa cagcctccgc cagcggcagg
     2341 agagcattcc gggggccccg caaaagcgtg gtgtttgcct ccaggtgcgc acgatgaccc
     2401 ccaagaagcc gaactcggca ctgcgcaaga tcgcacgcgt acgactcagc aatcagcagg
     2461 aagtcactgc ctacattccg ggcattggcc acaacctgca ggagcacagc gtggtcctcg
     2521 ttcgcggtgg ccgcgtccgt gacctcccgt cggtcaagta ccacatcatc cgcgggacgc
     2581 tcgacgccgc cggcgtccgc gaccgcaagc agggccgctc gctctacggc gccaagaggc
     2641 ccaagtaatg ccacgtcgag cacagagccc gcgcaaggac aagtacgcac cgaccctcac
     2701 ccgcaccaca gatcgcttcg ttgtgaagct gatgatggac ggcaagaagg gtctggcgga
     2761 gcgcatcctg cgccaggcac tcgctcgcgc ggaggcggcc acgcgccgcc cgggcctcga
     2821 ggtgctcgat gccgctctcc ggaacgcgac cccgatcatc gaggtcaagc cgcggcgcgt
     2881 cggtggtgcc acgtatcagg tcccggttga gatccgcggc gaccgccgcg tctcacttgg
     2941 tgtccgctgg ctcgtgcagg ccgcccgcaa gcgcagcggc aagtccatgt cagagaagct
     3001 tgccgctgag ttcgtcgacg cgatgaacgg cgtcggcgcc gcggtcaagc gccgcgaaga
     3061 cacgcacaag atggctgaag cgaacaaggc gttcagtcac ttcaagtggt aaccgcctcc
     3121 gtgaccagtc cccgggacac cgccaacagc gcctcgcgac aggtgccgct ggcgcgtacc
     3181 cgcaacatcg ggatcatcgc ccacattgac gcgggcaaga ccacggtcac tgaacgcatc
     3241 ctcttctaca caaagaagat ctacaagatc ggcgaagtcc atgagggcgc cgcaaccatg
     3301 gactggatgc cccaggagca ggagcggggc atcaccatca ctgccgccgc aacgaccgcg
     3361 ttctggggcg atcatcggat caacatcatc gatacgcccg ggcacgtgga cttcaccgtc
     3421 gaagtcgaac ggagcctccg agtgctcgac ggcgcggtcg tcgttttcga cggtgtggcg
     3481 ggcgtcgagc cccagtcgga gacggtctgg cgccaggccg atcgctacgg cgtgccgcgc
     3541 ttctgcttca tcaacaagct ggatcgcacc ggtgccgact tctggcgttg cgtccagtcg
     3601 atcgaggacc gccttggcgc caacgctgtg ccgatccaga tccccatcgg gcgcgaggat
     3661 aagtttcagg gcgtcatcga cctcgtcgag atgaaggccg tctactaccg cgacgacctg
     3721 ggtaaccaga tcgacgttgt cgacatcccg gccgagctca tggacgaggc ccagaagcat
     3781 caccacaaga tgatcgaggc cgtcgccgag atggacgacg atctgacgca caaatatctc
     3841 gaaggccagg acctcaccac ggacgagatc aggcgcggcc tccgcctggg caccctcagc
     3901 accaagatca tcccggtcct gacggggtcg gcgctcaaga acaagggcgt ccagaagatg
     3961 ctcgacgccg tcgtcgacta tctcccgtcg ccgcttgacg tgccgccgat ggtggccacg
     4021 gacccagaca ccggtgcgga gtctgtgcgg ccgccgagcg acgatgcgcc gttcagcgcg
     4081 ctcgccttca agattgcggc agatcccttc gtcggtaagc tggcgttctt ccgcgtgtac
     4141 tccggcaccc tcaagtcggg cagctacgtt ctcaatcccg tgaagggcaa gagggagcgc
     4201 atcggccgca tcctgcagat gcacgcgaac caccgcgagg agatcgagga ggtctttgcc
     4261 ggcgacatcg ctgc
//
LOCUS       DATLCO010000208         5421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6119412, whole genome shotgun sequence.
ACCESSION   DATLCO010000208 DATLCO010000000
VERSION     DATLCO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6119412"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VMZ33_05065"
     CDS             complement(<1..633)
                     /locus_tag="VMZ33_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HUR16632.1"
                     /translation="MSLRKDSERPRRANPPFGRLLIANRGEIALRIIRACRELGVESV
                     AVYSEADTQAVHVRAADSAVAIGPAPAGESYLRVDRIVDAAVTTGAQAIHPGYGFLSE
                     QPALAEACAAAGVVFVGPAVETLASLGDKLAARRRAAAAGVPVVPGTFEPIDVSGREG
                     VERARAEAARIGWPLLVKAAAGGGGRGMRRVDDPAELDDAITSAQRESEAA"
     gene            complement(645..2198)
                     /locus_tag="VMZ33_05070"
     CDS             complement(645..2198)
                     /locus_tag="VMZ33_05070"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HUR16633.1"
                     /translation="MNADRPEFARLEQLRRDSLLGGGERRIEQQHERGKLTARERLDL
                     LLDAGSFVELDAFVTHRASDFGLADEVYLGDGVVTGHGTIDGRLVFVFSQDFTVFGGS
                     LSEAHAEKICKVMDLAVEVGAPIIGLNDSGGARIQEGVVSLGGYADIFLRNTLTSGVV
                     PQISLVLGPCAGGAVYSPAITDFVVMVEGTSYMFVTGPDVVKTVTHEEVDREHLGGAS
                     IHTTISGVAHIAARDEAEAMSFGRRILEHLPQNNLSKPPRRETSDPRDRADVALNSVI
                     PAEANQPYDMHDVITRVVDDGDFLEIQPAWAANILVGFAHLAGRSIGIVAHQPAVLAG
                     ALDIDASDKAARFVRTCDAFNVPLLTFVDVPGFLPGVSQEHGGIIRHGAKLLYSYAEA
                     TVPKITVITRKAYGGAYDVMSSKHIRGDINLAWPTARIAVMGVEGAVNIIFRDELAAA
                     TDVEAERVRLAHEYEERFANPYIAASRGYIDDVIMPSETRPRVIAALEMLDNKRQTNL
                     PRKHGNIPL"
     gene            2347..3105
                     /locus_tag="VMZ33_05075"
     CDS             2347..3105
                     /locus_tag="VMZ33_05075"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR16634.1"
                     /translation="MAIANQKGGVGKTTTAINLAGSLAEQGFRVLCVDMDPQANLTVG
                     LGINLRNVELSMAEALVDPAVKLDEIILPTQTAGVDVAPSTIDLSATENVLFSAIGRE
                     QTLREALEGSALNNYDYVLIDCPPTLGLLTLNALVAADGVIIPVQTQYYALKGFSALL
                     NVITQIQTKGLNPRLRVMGLLPTFYDSRTILGRDMLQELRDMGDHHIFENMIKQTVRL
                     GEAPLVGRPVTSYAGNSEAARAYRGLAREVIELG"
     gene            3098..4111
                     /locus_tag="VMZ33_05080"
     CDS             3098..4111
                     /locus_tag="VMZ33_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUR16635.1"
                     /translation="MARPDFRGTAARRLAEERDLSPAILSLISPEASERPAGVRNVAL
                     DRITPNPEQPRMTFDEAALAELAASITEHGVLQPILVRPTDELYHYQLVAGERRWRAA
                     RAAGLKEIPALIEELDDETAMEIGIIENLQREDLSPLEEAMIYDRMTHEHGYSVRKLA
                     QKLGKDKGYIENRLRLAGAPTEIKQLVSLRKDTLSHAYELLKVEDSKKRRKLAEQVAS
                     GELSLVKLREKIEGRPRVVREPDEHAESVSENADDIDEMFAAPIARARSDEPVPDNAL
                     VLAKHNLAEALDELSDVLAMPELVGGIPDVDRQNLAKYLTIAKLRLENAITVVRSGRA
                     LDH"
     gene            complement(4108..4956)
                     /locus_tag="VMZ33_05085"
     CDS             complement(4108..4956)
                     /locus_tag="VMZ33_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HUR16636.1"
                     /translation="MPPPGGLNMNGIVRLVHPFPSLLNSALVLAVALVAGAEPRRAAL
                     LALGMLGLQFCIGAVNDLFDEHLDARSKPFKPIPAGLVSRQLATVVAVATGAGGLVLA
                     ELGVNLDPLVAVMYAVMLGAGLVYDARLKPTRFAWACFSVAFPILPVYAWYGATGALP
                     PRWEVLLPVAALAGPALQLANGLIDLESDSAAGLTTLPVRLGRHISIAVMTGIMAIIH
                     ALAWLTLPQTADPVVGVIAGIAGALALGGVMLSRMMPRIFREIGWSAQAASIAVLAFG
                     WLLSAA"
     gene            complement(4965..5330)
                     /locus_tag="VMZ33_05090"
     CDS             complement(4965..5330)
                     /locus_tag="VMZ33_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16637.1"
                     /translation="MTATGLSANPQEYRERLLEQSDEQVDAWVAELMRDISIRTGVRE
                     VLAKLRRALGTDDTGLQRLFATGGGPVATAGRTANGELMVPAISLFYFVIGIRALMPD
                     ARDRLISYLVDSFHEIVYI"
BASE COUNT         1039 a         1728 c         1723 g          931 t
ORIGIN      
        1 ggcggcctct gactctcgct gggcggacgt gatggcgtca tcgagctctg ccgggtcatc
       61 gacgcggcgc atgccacgac caccgccgcc ggccgcggcc ttgacgagca acggccagcc
      121 gatgcgtgcc gcttcggcgc gggctcgctc aacgccctcc cgtccggaaa cgtcgatcgg
      181 ctcgaaggtg cccggcacga cgggaacacc ggctgcggcg gcgcggcgtc gcgcggccag
      241 cttgtcgccg agggacgcca aggtctcaac ggctggacca acgaagacga cgccggcggc
      301 tgcacacgct tcagcgagcg ctggttgttc ggacaggaaa ccgtagccag ggtggatcgc
      361 ctgcgcgccg gttgtcacgg ccgcgtcgac gatccggtcc acacgcagat aggactcgcc
      421 cgccggggcc ggtccaatag cgaccgcgct gtcggcagct cgcacgtgaa ctgcctgggt
      481 gtccgcttcg ctgtagacgg cgaccgattc gacgcccaac tcgcgacatg cacggatgat
      541 ccgtagggct atctcccctc tattggcgat gaggagcctt ccaaagggcg ggttcgcccg
      601 gcgcggccgc tcactgtctt tgcgcaaaga cactcgccca gtcgctagag cggaatgttg
      661 ccgtgcttgc gcggcaggtt cgtttggcgt ttgttgtcga gcatctccag cgcggcgatg
      721 acgcgcggcc tggtctcaga cggcatgatc acatcgtcga tgtagccccg gctggcggcg
      781 atgtacgggt tagcaaaacg ctcctcgtac tcgtgggcga gccggacgcg ctcggcctcg
      841 acgtcagttg ccgcggcgag ctcgtcccga aagatgatgt tgacggcacc ctcgacgccc
      901 attaccgcga tgcgtgccgt cggccatgcc aggttgatgt cgccccggat gtgcttgctg
      961 ctcatgacgt cgtatgcgcc gccgtatgcc ttgcgcgtga tgaccgtgat cttgggcacg
     1021 gtcgcctcgg cgtacgagta cagcagcttc gcgccgtggc gaatgatccc gccgtgttcc
     1081 tgcgacacac cgggcagaaa gccgggcaca tcgacgaaag tgagcagcgg cacgttgaag
     1141 gcgtcgcacg tcctgacgaa tcgagcagcc ttgtcagagg cgtcgatatc cagtgcgccc
     1201 gccaacaccg ccggctggtg cgcgacgatg ccgatgcttc gaccagccag atgcgcgaag
     1261 ccgacaagga tgttcgccgc ccatgccggc tggatctcga ggaagtcgcc gtcatcaacg
     1321 acgcgcgtga tgacatcgtg catgtcatag ggctgattgg cctccgccgg gatgacagag
     1381 ttcaaagcga cgtccgctcg gtcgcgtgga tcgctcgtct cccgccgtgg cggcttggac
     1441 aggttgttct gcgggagatg ttccaggatc cgacggccga agctcatcgc ctcggcttcg
     1501 tcgcgcgccg cgatgtgcgc gacgccactt atcgtcgtat ggatcgacgc gccaccgaga
     1561 tgctcgcggt ccacttcctc gtgcgtgacg gtcttcacca cgtcgggtcc ggtcacgaac
     1621 atgtagctcg tgccctcgac catgacgacg aagtccgtga tcgccggcga atacaccgct
     1681 cccccagcgc acggcccgag taccaatgag atctgcggca cgaccccaga ggtcaatgtg
     1741 ttgcgcagga agatgtccgc gtacccgccg agggatacaa caccttcctg gatgcgcgcg
     1801 ccgcctgagt cgttgaggcc gatgatcggc gcacccactt cgaccgcgag atccatcact
     1861 ttgcagatct tctcggcgtg tgcctccgac agcgaaccgc cgaagacggt gaaatcctgg
     1921 ctgaagacga acacgagtcg accgtcgatc gtgccgtgcc ccgttacgac tccgtctccg
     1981 aggtagacct cgtccgctaa cccgaagtcg ctggcgcgat gagtgacgaa cgcatcgagc
     2041 tcgacgaatg agcctgcatc caacaacaga tcgagacgct cgcgagcggt gagctttcca
     2101 cgttcatgct gttgctcgat acgccgttcg ccgccgccaa gcagcgaatc gcgacgaagt
     2161 tgctctagac gcgcgaactc gggacggtca gcattcatgg ggcggcctat cgtagcaacg
     2221 ccgctgtctc cagttctgtc cacagaatcg gtgcacaagt tcgacatcct gtggataacc
     2281 ggactttgtc cggaaggcca ttcttgctag ccttcgacct gaggaggacg tcagctgggc
     2341 aaagtaatcg cgatcgcgaa ccaaaagggc ggagtcggca agacgacaac cgccataaat
     2401 cttgccgggt ccttggccga gcagggcttc cgcgtccttt gcgtcgacat ggatccgcag
     2461 gccaatctga ccgtcggcct gggcatcaat ctccgcaatg tcgagctctc aatggctgag
     2521 gccttggtcg atcctgcggt gaagctcgac gagatcatcc tgccaacgca gacggctggc
     2581 gtggatgtcg caccctcgac aatcgatctc tccgcgaccg aaaacgttct gttcagcgcc
     2641 attgggcgtg agcagacgtt gcgcgaggcg ctcgaaggct cggccctgaa caactacgac
     2701 tacgttctga tcgactgccc gccgactctc ggccttctga ccttgaatgc tctcgtggcg
     2761 gctgacggcg tgatcatccc ggtgcagact cagtactacg cgctaaaggg cttctctgcg
     2821 ctgctcaacg tcatcaccca gattcagacc aagggattga atccgcggct gcgcgtcatg
     2881 ggtctgttgc caaccttcta tgacagccgc acgatcctcg gtcgcgacat gcttcaggag
     2941 ctgcgcgaca tgggcgacca ccacatcttc gagaacatga tcaaacaaac agttcggctg
     3001 ggcgaggcgc ctctcgtcgg acgcccggtg acgtcgtatg ccggaaactc ggaagcggcg
     3061 cgggcttatc gcgggctcgc gcgggaggta atcgagcttg gctagaccag attttcgtgg
     3121 caccgccgcg cggcggctgg ccgaggaacg tgacctctcg ccggccatcc tgtcgctgat
     3181 ctcgccagag gcatctgagc gccccgcagg cgtgcgcaat gtggcgctgg atcggatcac
     3241 gcctaacccc gaacagccgc gcatgacgtt cgatgaagct gcgctcgctg agctggctgc
     3301 gtcaatcacg gagcacggcg tgctccagcc gattctcgtg cggccaaccg atgagctgta
     3361 tcactaccag ttggtggctg gtgagcgacg ttggcgggcc gcccgagccg cgggtctcaa
     3421 ggagatcccg gccctcatcg aagagctcga cgacgagacc gcgatggaga tcggaatcat
     3481 cgagaacctg cagcgcgagg atctgtcgcc gctcgaggag gcgatgattt acgaccgcat
     3541 gactcatgag catgggtaca gcgtccgcaa acttgcccag aagctgggca aggacaaggg
     3601 ctatatcgaa aaccggttgc gcctggctgg tgccccgact gagatcaagc agttggtgtc
     3661 tttgcgcaaa gacacgctgt cgcacgccta cgagctgctg aaggttgagg actccaagaa
     3721 gcgtcggaag ttggccgagc aagttgccag cggcgaactg agcctggtca agctgcgcga
     3781 gaagatcgaa ggtcggccgc gcgtcgttcg cgagcctgac gaacacgccg agtcagtttc
     3841 tgaaaatgca gacgacatcg atgagatgtt cgctgcgcca atagctcgcg cgagatctga
     3901 cgagcccgtg cctgacaacg ctttggttct agccaagcac aacctcgctg aggctctcga
     3961 tgaattgtct gatgtgctcg ccatgccgga gctcgtcggc gggattccgg acgtggaccg
     4021 tcagaacctg gccaagtacc tgacgatcgc caagctcaga ctcgagaacg cgatcaccgt
     4081 tgtgaggagc ggccgagcac tcgatcacta ggcggcgctg agcaaccaac cgaacgccag
     4141 gacagcgatc gacgccgcct gggcgctcca accgatctcg cggaagatcc gcggcatcat
     4201 tcgggacagc atcaccccac ctagtgccag cgcgcccgcg attccggcta tgacgccgac
     4261 gaccggatcg gcggtctgcg gcagagtcag ccacgccaac gcatgaatga tcgccatgat
     4321 gccggtcatg acggcaatgc tgatgtggcg acccaggcgg acgggcaaag ttgtgagccc
     4381 tgcggccgag tctgattcga gatcgatcaa tccgttcgcg agctgcaggg cggggccggc
     4441 cagggcggcc actggcagga ggacctccca ccgtggagga agcgcacccg tcgctccgta
     4501 ccacgcgtag acgggcagaa ttgggaaggc gacggagaaa caggcccacg cgaaacgcgt
     4561 tggcttgagc cgggcgtcat agacgagacc agcaccgagc atcaccgcat acatgactgc
     4621 gaccagcgga tccagattca cgcccagctc agccagtacc aggcctccag cgccggtcgc
     4681 cacggcgacg acagtcgcca actgtcggct cacgagaccc gccggaatgg gtttgaacgg
     4741 ctttgaccgc gcgtccaggt gctcgtcgaa gaggtcgttg acagccccga tacagaactg
     4801 cagaccgagc attccgagcg ccagtaacgc cgcccgccta ggttcggcgc cggctaccag
     4861 cgcgacggca agcaccaggg cgctgttgag cagcgacgga aacgggtgta cgagccggac
     4921 gatgccgttc atgttgaggc ccccgggcgg gggcactgcc ccagctagat gtagacgatc
     4981 tcgtgaaagc tatcgacgag gtacgagatc agccggtccc gagcgtcggg catcagcgcc
     5041 cggatgccga tgacgaaata gaagagcgag atcgcgggga ccatgagctc tccattcgcg
     5101 gtacgaccgg cagtggccac cgggccaccg cccgttgcga acaaccgctg cagccccgtg
     5161 tcatcggttc cgagggcgcg gcgcagcttc gccagcacct cccgcacccc ggtgcggatg
     5221 gagatgtcgc gcatgagctc ggcgacccag gcgtcgacct gctcgtccga ctgttcgagc
     5281 aggcgttcac ggtattcctg gggatttgcg gaaaggccgg ttgcggtcaa gaaaaggcgc
     5341 cctcctgatg agggcgccaa tctaactcat ctacgcctga gatcaggccg cggtcgctcg
     5401 gggtcgaaag atccggacca g
//
LOCUS       DATLCO010000209         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_317650, whole genome shotgun sequence.
ACCESSION   DATLCO010000209 DATLCO010000000
VERSION     DATLCO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317650"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VMZ33_05095"
     CDS             <1..247
                     /locus_tag="VMZ33_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16638.1"
                     /translation="KVEMRVHVLGSVRTLRGLENAGGWFLRADKGLKMIPGGVRDTGV
                     NYECDLLVLRPGAASQHLDATFRVEAGKITAINLTPR"
     gene            complement(357..1628)
                     /locus_tag="VMZ33_05100"
     CDS             complement(357..1628)
                     /locus_tag="VMZ33_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HUR16639.1"
                     /translation="MPAPRTVAHQERYCLSPNFSGCPVFQAWAVRAAARPVPIPGGVS
                     ASTRSLEPAHEVETVRSAGTGGEGSAAPAAQPDEPDVVPAADPLAPIPPVLPRPPVVA
                     VGSDGSGLDEPPVPEGPLPAHDEMWPDSMGAPIVPETGALGVPTPSRASGEADMPIQT
                     DTTWSAAVSGRPPAAQEQLSVFDAPMAPIRSPSVDPEAPAPTFASTLPDESFAPLAVA
                     SSAEPPHASPDYDNVEDEAPTVPAFLTGRSARPRPPVSGDHLDRGDLVPSWDLDGRFG
                     GEPGPGQTTRADGGGMGTVLTTVAVIAIIGLGILAVLLIPGVLGGNGSQPTRRPTNLP
                     TISTTAQATTVAVLPTAAGEPTPADVQPTPEPTPEATPIFYTIKRNDTLARVARRNGT
                     TVDDILAANPQIPGANAIQVGQVIVLPPPTE"
     gene            complement(1763..>2412)
                     /locus_tag="VMZ33_05105"
     CDS             complement(1763..>2412)
                     /locus_tag="VMZ33_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16640.1"
                     /translation="ARFPVQWVIRPQTDEHHDYRGYAGQVAAGVFTKGDEVMILPSGK
                     RTRIKRIETMDGELESAAPPMSVSILLEDDIDISRGDMICRPHNQPTVAQELDAMICW
                     MADEPLREGRKYLLKHTTRTVKAVVGDLRYRMDVNSLHRDQGSTELGLNEIGRVQLRT
                     SGPLLLDPYDLSRTTGGFVLIDETSFDTVGAGMVIHAGPEQQQMSGDDISESLAA"
BASE COUNT          392 a          820 c          816 g          384 t
ORIGIN      
        1 gaaggtcgag atgcgggtcc atgtgctcgg cagcgtgcgc acgctacgcg gcctcgagaa
       61 cgccggtggc tggttcctcc gcgccgacaa gggcctgaag atgatccccg gcggcgtccg
      121 cgacacaggc gtgaactacg aatgcgacct gctcgtgctt cggcccggcg ccgcctcgca
      181 acacctcgat gccactttcc gggtcgaggc aggcaagatc acggcgatca acctaacccc
      241 gcggtagccc cggcccgcgg catcacagtt tgcatgtggt gcaaaccaga accgtggccc
      301 cggaacccca tcgttgattg cacccggtgc agacgccaca ggtgggtgcc cgatcgctac
      361 tccgtcggcg gtggcaggac gatcacctgc ccgacctgga tcgcattcgc gcccgggatc
      421 tgggggttcg cagccaggat gtcatccacg gtcgtcccgt tccgacgagc aacgcgcgcc
      481 aacgtgtcgt tgcgcttgat cgtgtagaag atcggggtgg cctccggtgt cggctcgggt
      541 gtcggctgaa cgtccgctgg cgtcggctcg cccgccgcgg tcggcagcac ggcaaccgtg
      601 gtcgcttgcg ccgttgtcga gatagtcggg agattcgttg gccgacgggt gggctgggac
      661 ccattgccac cgaggacgcc agggatcagg agcacggcca ggatgcccag gccgatgatg
      721 gcaatgactg ccaccgtcgt cagcacggtg cccatcccgc cgccgtcggc gcgggtggtt
      781 tgaccgggtc ccggttcgcc cccgaaacga ccatcgagat cccacgaggg cacgagatcg
      841 ccacgatcga ggtggtcgcc gctaaccggt ggccgtggcc gggccgagcg acccgtcagg
      901 aaagcgggca cagtcggggc ttcgtcctcg acattgtcat agtcgggcga ggcatgcggc
      961 ggctcagcgc tgctggccac ggccagcgga gcgaacgact cgtcaggcag agtggacgca
     1021 aaggtcggtg ccggcgcttc aggatccacc gacggtgacc ggatcggagc catcggcgcg
     1081 tcgaagacgc tcagctgctc ctgcgccgca ggtggtcgcc ctgaaacggc cgccgaccac
     1141 gtggtgtcgg tttggatcgg catgtctgct tcgcccgacg ccctcgacgg cgtcggcaca
     1201 cccagcgcac ccgtctcagg aacgatcggc gcgcccattg agtcgggcca catttcgtcg
     1261 tgggcgggca gcggcccctc gggcacaggc ggctcatcaa gacctgatcc gtccgatccg
     1321 acggcaacca ccggcgggcg aggcaggacc ggcgggatcg gcgcaagcgg gtcagcggcc
     1381 ggcacgacat cgggctcgtc gggctgggcg gcaggcgcgg cactaccttc gccacccgtc
     1441 ccggccgaac gcaccgtttc gacctcgtgt gccggctcca ggctgcgcgt cgacgcggac
     1501 actcccccag gaatgggaac aggccgtgcc gccgcccgca cggcccacgc ctggaagacc
     1561 gggcagcccg agaagttcgg ggagaggcaa tacctctcct gatgagcgac ggtacgcggc
     1621 gcgggcacct gttcggcgta acagcggtga cggtaatcag gccgctcaga tcgccgatcg
     1681 cgatccagct ccagggctac gaatgggcag gcgctggccc ctgcctcgaa cgatgatccc
     1741 ctcgccgcca tcgcggcaga tgttatgccg cgagcgactc cgaaatgtcg tcccctgaca
     1801 tctgctgctg ctccggcccg gcgtgtatga ccatcccggc accgaccgtg tcgaacgatg
     1861 tctcatcgat gagcacgaag ccgcccgtcg tccgcgacag atcgtacgga tccaggagga
     1921 gcgggccgct cgtgcgcagt tgcacgcgcc cgatctcgtt cagcccaagc tcggtcgaac
     1981 cctggtctcg atggagcgag ttgacatcca tccggtagcg caggtcgccg acgacggcct
     2041 tgaccgtccg cgtggtgtgc ttgaggagat acttccggcc ttcgcggagt ggctcatcgg
     2101 ccatccagca gatcatcgcg tccagctcct gggcgacggt cggctggtta tgcgggcggc
     2161 agatcatgtc gccgcgactg atgtcgatgt cgtcctccag cagaatggaa accgacatcg
     2221 gcggtgccgc gctctcgagc tcgccgtcca tcgtctcaat ccgcttgatg cgcgtccgct
     2281 tgcccgacgg caggatcatc acctcgtcgc ccttggtgaa gacgccggca gcgacctgac
     2341 ccgcgtagcc gcggtaatcg tggtgctcgt cggtctgcgg acggatgacc cactggaccg
     2401 ggaagcgcgc at
//
LOCUS       DATLCO010000210         4609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6123325, whole genome shotgun sequence.
ACCESSION   DATLCO010000210 DATLCO010000000
VERSION     DATLCO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6123325"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VMZ33_05110"
     CDS             <1..1164
                     /locus_tag="VMZ33_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HUR16641.1"
                     /translation="EIPNHTGEIVGFRPLLEDVAVGDSASRLMFALGRDVSGKGYAVD
                     LARMPHLLIAGATGSGKSVCVNALITSLLMRAAPDEVRLVLIDLKRVELAAYRDVAHL
                     EVPVIVEAHEARAVLTNLVHEMEQRYELLAAAMERNITAYNVSPRRVGKPMPFMVVVI
                     DELADLMLREGRKVEEPVVKIAQKARAVGIHLVLATQRPSVNVVTGLIKANVPSRIAF
                     AMASNVDSRTVLDAPGAEDLIGRGDMLYQPADLPRPIRLQGVFVSDAEVHAVTEFWRQ
                     QSEPIYNRDLLQGGEVDDDGEGGQFSWLAKSAGDEMAARAAELITTTGRASTSMLQTK
                     LKVGFNRATRLMDELEKHGIIGPQDPRNPAVPRQVYGSDNWLRGAADADGDLS"
     gene            1310..2839
                     /locus_tag="VMZ33_05115"
     CDS             1310..2839
                     /locus_tag="VMZ33_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HUR16642.1"
                     /translation="MTARHQLRIGNRRLGRDRTPIDATPAPPVGETLQIARERKGVDL
                     FRAERDTKIRLRYLAALEDGAYDDLPAPVYTKGFLRNYAIYLGLEPEEILERWRDEME
                     SLRESEQIAVAPPPQPIAAPARSFHVSSAIFFRGLVAVVIVAFVGYLAIQLLRFAETT
                     PVALTNPPNVVSTVNDDHVALEGTAGRGSLITIRGPGGDLWNPRADEDGHWAQEVPLS
                     LGRNDFTVIATDAVTNRESKPLLLTINVPLPSRSPNASPSVPPPVALVLALDDPEDGF
                     LSSDGIVVVRGTTTGSRITITSTYLGEPLVTAPQTLAPGATPTLAPTPAGEPTPSPTP
                     SPMPGPSEVPPSSAGPSSTPSIAPSLAPGATGPSRDITVSDAGTFDETMNFGVGRWEL
                     TVTTNGIGVDPLVETRTITVGPPPTTGIVLAVSVEGRRSWVRLQADGARIGGFGTLEI
                     GFQRTVTATSEICLYTGNAGGLHLTLNGLDLGLLGRAGQVGSWLIRAGEAPTPTESSC
                     G"
     gene            2839..3360
                     /locus_tag="VMZ33_05120"
     CDS             2839..3360
                     /locus_tag="VMZ33_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HUR16643.1"
                     /translation="MDEAADELAALVMMGARIQSVLIERGLTLATAESCTGGLIGYIL
                     TETTGSSAYYVGGLISYTNDLKEHELGVDSRTLQKHGAVSAQACVAMADGARKRYGAT
                     IGLSVTGIAGPEGGSEQKPVGLTYVGVADDAGHDVRRNVWGDNNRHVNRVRSAEAALG
                     LLLERLGTEPQTA"
     gene            3357..>4609
                     /locus_tag="VMZ33_05125"
     CDS             3357..>4609
                     /locus_tag="VMZ33_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HUR16644.1"
                     /translation="MTRTSDDIGQGLAAARQARPIAARERIHVVGAAGAGASAAALLA
                     HHAGATVTGCDPGGPSPYTAALQAAGIPVAWEHDAAHVAAGVDRLAVTKALTSVDPGH
                     PELHAARAAGVPLEPWQQVVADAAATSGQRLVAIAGTHGKSTTTGWLVDVLMRDGRDP
                     SAFVGALLPASVSGTAPATARWGNGDLFVVEADEYAGNFDPYRPSVALLLNAEWDHPD
                     VFADDEAVLSAFEAWIRHSLPDATLVANMEDPGVARIVQRLSDWPGEIVAAPPTVPLG
                     WDVGLPGPANADNARMVIGAARVLGVTDTALREGVADYVGVGRRMEVKGDVGGVVVID
                     DYGHHPTAIAKTLEAVRSRYPGRRVWAVYEPLTYHRTAAMLGEFARVLAAADEVAIAD
                     IWAGRDPDTTLTSASALAEYVRAVGG"
BASE COUNT          754 a         1577 c         1552 g          726 t
ORIGIN      
        1 gagatcccca accacacggg cgagatcgtg ggcttccggc cactcctcga ggacgtggcc
       61 gtgggcgatt cggcctcgcg cctgatgttc gcccttggtc gtgatgtgag cggcaagggc
      121 tacgcggtgg acctcgcgcg catgccgcac ctgctcatcg ccggcgcgac gggctcgggc
      181 aaaagcgtgt gcgtcaacgc gttgatcacg agcctgctga tgcgtgccgc gcccgacgag
      241 gtccggctgg tgctcatcga cctcaagcgc gtcgagctgg ccgcgtatcg cgatgtcgcg
      301 cacctcgagg tgccggtcat tgtggaggcg catgaggcgc gggcagtgtt gacgaacctt
      361 gtgcacgaaa tggagcagcg ctacgagctg ctggcggctg ccatggagcg caacatcacc
      421 gcctacaacg tgtcgccacg acgcgtcggc aagccgatgc ccttcatggt cgtcgtcatc
      481 gacgaactgg ccgacttgat gctccgcgaa ggacgcaagg tcgaggagcc tgtcgtcaag
      541 attgcccaga aggcgcgcgc cgtcggtatc cacctggtcc ttgcaacgca acggccgagc
      601 gtcaacgtcg tgaccggact gatcaaggcc aacgtgccat cgcgcatcgc gttcgccatg
      661 gcgagcaacg tcgattcgcg gacggtcctc gacgcgccgg gcgccgagga tctcatcggt
      721 cgcggcgaca tgctctacca gccggctgac ctgccgcgtc cgatccgcct tcagggtgta
      781 ttcgtcagcg atgccgaggt ccacgccgtg accgagttct ggcgccagca atccgagccg
      841 atctataacc gcgacctgct tcagggcggc gaggtagacg acgatggcga gggcgggcag
      901 ttcagctggt tggcgaagtc cgccggagac gagatggcgg ctcgcgcagc ggagctcatc
      961 accaccaccg gccgggcgag cacctcgatg ctgcagacga agttgaaggt cgggttcaac
     1021 cgagcgacgc gcctcatgga cgagctggaa aagcacggga tcattggccc gcaggatcca
     1081 cgcaaccctg cggtaccgcg ccaggtttac gggtcggaca actggctccg tggcgccgcg
     1141 gatgccgatg gggacctctc ctaggacctc tctcccgatc ggcggaagcg gtccacgggg
     1201 tagactggac gtccgcgtca ggatggcgcc gcaagcggcg tcgcgcgcag ccgggcggca
     1261 gcccgagcgg agcaagccaa tcgagcgcta gaggcctccc ttacccgcga tgaccgcccg
     1321 ccatcaactg cgcatcggta atcggcgtct tggccgtgac aggacgccta tcgacgccac
     1381 tcccgcgccg ccggtcggcg agaccctgca gatcgcgcgc gagcgcaagg gcgtcgacct
     1441 gttccgagcc gaacgtgaca ccaagatcag gctgcgttac ctcgcggcgc tcgaggacgg
     1501 cgcatatgac gatctccccg cgccggtcta caccaagggc ttcctgcgca actacgcgat
     1561 ctatctgggc ctagagcccg aagagatcct ggagcgctgg cgcgacgaga tggagtcgct
     1621 gcgtgagagc gagcagatcg cggtggcgcc gccaccgcag ccgatcgccg cccctgcccg
     1681 cagcttccat gtctcgtcgg cgattttctt ccgagggctc gtagcggtcg tcatcgtggc
     1741 tttcgttggc tacctcgcga ttcagcttct ccgcttcgcc gagacaacgc ccgtagcgct
     1801 gaccaatcca cccaacgtcg tgagcacggt caacgacgac cacgtcgcac tggagggcac
     1861 ggccggccgt gggtcgctca tcaccattcg cgggcccggc ggcgacctct ggaacccgcg
     1921 tgccgacgag gacgggcact gggcgcagga agtgccgctc tcgttgggtc gaaacgactt
     1981 cacggtcatc gcaacggacg cggtcacgaa ccgtgagtca aaacccctgc tgctcaccat
     2041 caatgtgccg ctgccgtccc ggtcgcctaa cgcgtcgcca agcgtcccgc caccggtggc
     2101 tcttgtcctt gccctcgacg atccagagga cggcttcctg agcagcgacg gcatcgttgt
     2161 cgttcgtggc acgacgactg gatcgcgcat cacgatcacg agcacctacc tgggggagcc
     2221 cctcgtcaca gcgccacaga cactggcgcc gggcgccacc ccgaccctgg ctcccacgcc
     2281 ggccggcgag cccacaccca gtcccacgcc atcgcccatg cccggcccga gcgaggtacc
     2341 accatcgtcg gccgggccgt cctccacgcc ttccatcgcg ccgtcgctgg cacccggcgc
     2401 aactgggccc tcacgggaca tcaccgtctc tgacgcgggc acgttcgacg agacgatgaa
     2461 tttcggcgtc ggccgttggg agctcacggt cacgaccaat ggcatcggcg ttgacccgct
     2521 cgtcgaaacg cgcacgatca ccgtcggtcc gccacccacc accgggatcg tcctggccgt
     2581 gagcgtcgag gggcgccgat cctgggtacg cctgcaggca gacggagctc gtatcggcgg
     2641 ctttggcaca ctcgaaatcg gattccaaag gacggtcacc gccacgagcg agatatgcct
     2701 ttacaccggc aacgccggtg gcctgcacct caccttgaac gggctggatc tcggcctgct
     2761 gggtcgcgcc ggtcaggtcg gaagctggct catccgcgct ggcgaggccc cgaccccgac
     2821 cgagtcgtcc tgcgggtaat ggacgaggcg gccgatgagc tggcggccct ggtgatgatg
     2881 ggcgcccgca tccagtcggt cctgattgag cgtggcctga ccctggcgac cgcagagtcg
     2941 tgcacaggcg ggctgatcgg ctacatcctg accgagacga ccggctcgtc cgcgtactac
     3001 gttggcggcc tgatcagcta caccaacgac ttgaaagagc atgagctcgg tgttgacagc
     3061 aggacgctcc aaaagcacgg cgccgtgtca gcacaggcgt gcgtggcaat ggccgacggc
     3121 gcacgtaagc gctacggcgc cacgatcggg ctgtccgtca cgggcattgc tgggccggag
     3181 ggtggcagcg agcagaagcc ggtgggcctg acctacgtgg gtgttgccga tgacgccggc
     3241 cacgacgtcc gacgcaacgt ttggggtgac aacaaccggc acgtcaaccg cgtgcgcagc
     3301 gctgaggcag ccctgggcct cttgctcgag cgcctcggta cggagccgca gacggcttga
     3361 cgcgtacgtc cgacgacatc gggcaggggc tggccgcagc acgtcaggcg cggccgatcg
     3421 ccgcccgtga acggatccac gtcgtcggcg cagccggtgc cggagcctcc gctgccgcat
     3481 tgctggccca tcacgcgggt gcaaccgtca ccggctgcga tccaggcgga ccgtcgccat
     3541 acacggcagc gctccaagcg gccgggattc ccgtcgcgtg ggagcacgac gctgcccatg
     3601 tcgcggccgg cgtcgatcgg cttgccgtca ccaaggcgtt gacctcggtc gaccccggcc
     3661 atcccgagct gcacgcggcg cgcgcggccg gggtaccgct ggagccgtgg caacaggtcg
     3721 tcgccgacgc cgccgccacc tccggccagc gactcgtcgc catcgccggc acccatggaa
     3781 aatccaccac gacgggctgg ctggtggacg tgctgatgcg cgacggtcgg gatccgtcgg
     3841 cgttcgttgg tgcgcttctg cccgcgtccg tgtccgggac agcgcccgca acagcacgtt
     3901 ggggaaacgg cgaccttttt gtggttgagg cggacgagta cgccggcaac tttgatccgt
     3961 accggccgtc cgtggccttg ctactgaacg ccgagtggga ccaccccgat gtgttcgccg
     4021 acgacgaggc ggttctcagc gcgtttgagg cgtggatccg ccattcactc cccgacgcga
     4081 cgttggtcgc caacatggag gatccaggcg tcgcccggat cgtccagcgt ctgtccgact
     4141 ggccgggcga gatcgtggct gcgcccccaa cagttccgct gggctgggac gtcgggctcc
     4201 ccgggccggc caacgccgac aacgcgcgca tggtcatcgg cgccgctcgg gtcctgggtg
     4261 tcacggacac ggcgctgcgc gagggtgttg cggactacgt gggggttggc cggcggatgg
     4321 aggtcaaggg cgatgtcggc ggcgtagtcg tgatcgacga ctatgggcac cacccgactg
     4381 cgatcgccaa aacgctcgag gcggtccgct cgcggtatcc cgggcggcgt gtgtgggctg
     4441 tctacgagcc actcacatat caccggaccg ccgcgatgct cggcgagttt gcccgcgtgc
     4501 tggcagcggc cgatgaggtg gccattgcgg acatctgggc cggtcgcgat cccgatacga
     4561 ccctgacgag cgcctccgcg ttagccgagt acgtcagggc agtcggcgg
//
LOCUS       DATLCO010000211         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6871057, whole genome shotgun sequence.
ACCESSION   DATLCO010000211 DATLCO010000000
VERSION     DATLCO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6871057"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VMZ33_05130"
     CDS             complement(<1..276)
                     /locus_tag="VMZ33_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16645.1"
                     /translation="MRGVVMAVLVTAALVASPSIASAQIGIGARMAWVTPDTDVEVDR
                     VRFIGGQIRLLSSKLGLEVAMDHHSEEFPLVNQKVTETPIQASVLLAL"
     gene            complement(491..883)
                     /locus_tag="VMZ33_05135"
     CDS             complement(491..883)
                     /locus_tag="VMZ33_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR16646.1"
                     /translation="MKIKLTSVHVDDQDKALRFYTDVLGFVKKNDFSQGGYRWLTVAS
                     PEDPDGTELQLARNDNPAARAYQQAMFQQRQPAAMFFTDDLQADYERMKASGAVFGMP
                     PTDVTASTIAMLDDTCGNLIQLTQLKRW"
     gene            complement(970..1371)
                     /locus_tag="VMZ33_05140"
     CDS             complement(970..1371)
                     /locus_tag="VMZ33_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HUR16647.1"
                     /translation="MKMKRTSVTAFWIATALLCLQLAFTAYAQLRLPQVAQAFAHLGF
                     PDYFRIELALAKLLGVILLLAPVPARLKEWAYAGFAITLGSALIAHFSVGDGPEAWGW
                     AAGTAVLWALSYFFWRRLDSIPVTMRHARAA"
     gene            1644..>2078
                     /locus_tag="VMZ33_05145"
     CDS             1644..>2078
                     /locus_tag="VMZ33_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HUR16648.1"
                     /translation="MGERDDLAGRFEEHRSHLRGVAYRMLGSFNEVEDAVQEAWLRLS
                     RTDAAAIDNLGGWLTTVVSRVCLDMLRSRQSRREEPFDADRLEGPAIGARGSGSIVSD
                     SRASSGPVASERRESRDPEQEALLADSVGLALMVVLDRLAPAE"
BASE COUNT          338 a          646 c          750 g          344 t
ORIGIN      
        1 cagcgccagg agcaccgacg cctggatcgg cgtctcggtg accttctggt tgaccagagg
       61 gaactcctcg gagtggtggt ccatggcgac ttcgaggccc agcttgctcg agaggaggcg
      121 gatctggccg ccgatgaacc gcacccgatc gacctcgacg tccgtatcgg gggtgaccca
      181 cgccatgcgc gcgccgatgc cgatctgggc cgaggcaatg gacggactgg caaccagggc
      241 cgccgtcacg aggaccgcca tgacgacgcc gcgcatgggg gcggtgcaga ttgttcgcat
      301 tgttcgagac tccacgcgct tgcacgcggc ggcgggtgcc gccggagcga tgcaggggct
      361 tcggaatctt atcggcacga gacgggcggg aattcagcgc gaggtcgcga gcggcgtgac
      421 ggatcgcaag tgttcagcga cgcacgcggt ttccgcgcgt ccgccggctg tgcaagtacg
      481 cccggttctg ctaccagcgt ttcagctgtg tcagctgaat caggttgccg cacgtgtcgt
      541 ccagcatggc gatcgtggac gcggtcacgt ccgttggcgg catgccgaac acggcgccgg
      601 acgccttcat gcgctcgtag tccgcctgga ggtcgtcggt aaaaaacatg gccgcgggtt
      661 ggcgctgctg gaacatcgcc tgctggtacg ccctggcggc cgggttgtcg ttgcgcgcga
      721 gctggagctc cgtgccgtcc gggtcctccg gggacgcgac ggtgagccag cgatatccgc
      781 cctggctgaa gtcgttcttc ttcacgaagc cgaggacgtc ggtatagaag cgcagggcct
      841 tgtcctggtc gtcgacgtgc acgctggtca gtttgatctt caccggttct ctccttgggt
      901 cgctttgtcg cggtgtccgt cactgctatg acggatcgcg gccggaaaat gtgacgatga
      961 gcgagccgct tacgcagccc ttgcgtgacg catggtcacc ggaatggagt ccaggcggcg
     1021 ccagaaaaag taggacaggg cccacagaac ggcggtgcca gcggcccagc cccatgcctc
     1081 cgggccatct cccactgaga agtgcgcgat gagcgcggag ccgagggtga tggcgaagcc
     1141 ggcataggcc cattccttga gccgtgccgg caccggggcc agcagcagga tcacgccgag
     1201 cagcttggcc agcgcgagct cgatgcggaa gtagtcgggg aagccgaggt gcgcgaacgc
     1261 ctgcgccacc tgcggcagcc gcagctgcgc gtaggcggtg aaggcgagtt gcagacaaag
     1321 caacgcggtc gcgatccaga acgccgtaac actggtccgt ttcatcttca ttggttctcc
     1381 ttatgcctgc ctcgccgaag ccgcccgcga agtccgcggc gaaggcgggt cgcgccgtag
     1441 cctcgtcgaa gccgtggcga aggctccctc gtcgccgagc cgcttcgaag gcgggggcct
     1501 accccccgaa gccgttcgcg aagtcgtcgg cgaaggcggg cctgcccccc gaagccgtct
     1561 gcgaagtcat cggcgaaggg gggtcacact cttcaacgtt catccgtcag agctatgacg
     1621 gaacgggatg ggagaatgtg acgatgggcg aacgcgatga cctggccggg cggttcgagg
     1681 agcaccggag ccacctgcgc ggcgtggcct accggatgct cgggtcgttc aacgaggtgg
     1741 aggacgccgt ccaggaagcg tggcttcggc tcagccgcac cgatgcggcg gccatcgaca
     1801 accttggcgg ctggctgacc accgtggtgt cgcgggtgtg cctcgacatg ctgcgatcgc
     1861 gccagtcgcg tcgcgaggag ccgttcgacg cggacaggtt ggagggtccc gcgatcggcg
     1921 cgcgcggtag cggttctatc gtgagcgata gtcgcgcgtc gagcggccct gtcgcgagcg
     1981 agcgaaggga gtcgagggat cccgagcagg aagcgctcct cgccgattcg gtcggcctcg
     2041 cgctgatggt cgtgctcgac cggctggcgc cggcggag
//
LOCUS       DATLCO010000212         7218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4799707, whole genome shotgun sequence.
ACCESSION   DATLCO010000212 DATLCO010000000
VERSION     DATLCO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4799707"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1726
                     /locus_tag="VMZ33_05150"
     CDS             395..1726
                     /locus_tag="VMZ33_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HUR16649.1"
                     /translation="MNDPIDPAYSGGPQPPVARPAVAMAGLLVVLLVFVVGMAVGSSG
                     ILGPRSTPRPSPVAVQPTPAPGSTPAPLPSNVPGNFALFWQALQTVRDSFVGRGELTD
                     EQLTNGAIRGMIEALGDTGHSVFLTPEAVAAENESLGGTVVGIGVLLGERNAQVVVVS
                     VISGGPADDAGIRSGDRILTIDGASVEGLAPEEVAPRVRGEAGSTVVLNIERPATGED
                     LEFSIVREELRFPAAYWTMVPGTNIGFLRLVQFSTGSADELRAARDEAIDAGAESLIL
                     DLRSNPGGYVDEAVDIASLFLSDRVVFLSEDAAGTRTEVRTNSSVEATDLPLVLLIDE
                     GSASSSEIVTGAIRSAGRAELIGTTTFGTGTVLQTFPLTNGSAIRLATERWLTPDGEL
                     IFGQGIDPTTAVELPADQTPLDPIDVRDMPADEIDSLPDDQLRRAIELLTQ"
     gene            1816..2796
                     /locus_tag="VMZ33_05155"
     CDS             1816..2796
                     /locus_tag="VMZ33_05155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HUR16650.1"
                     /translation="MVEIGYALSSEEHTPLDMVRWAERAEQAGFGFALISDHYHPWID
                     RQGHSGFVWGLIGAISQRTEKLVLGTGVTAPIIRTHPAIIAQAAATAACLMPGRFFLG
                     VGSGENLNEHILGDKWPPADIRLEMLEESIDVMRTLWQGGVQDYDGSYYVVENARVYD
                     LPDDEIQVMVAASGEKASQLAGRVGDGLIGTSASTDTVTAFEKAGGSGKPRYGQMSVC
                     WAETEEEAVRVAHEVWPIAGMGGQLGQELALPALYEAVAELVTPDQIKESIVCGPDAE
                     PVRKKIQEYADAGYTHVYLHQIGPDQEGFFRFAERELLREPVAAGLAGAR"
     gene            complement(2793..2999)
                     /locus_tag="VMZ33_05160"
     CDS             complement(2793..2999)
                     /locus_tag="VMZ33_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16651.1"
                     /translation="MRAARAHNLFVIGAGFWSLVWATIALTSLFSADGRLQFMNIFLA
                     GVAWAAPLGVAVALYSVLRDLSTR"
     gene            3058..3969
                     /locus_tag="VMZ33_05165"
     CDS             3058..3969
                     /locus_tag="VMZ33_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HUR16652.1"
                     /translation="MTERSKRSRVALIVNPVAGGGRGARVVGEAVYELRKRGVEPRII
                     VSVSPDEPPGDARRAANDGADLVIAVGGDGHAAAVASGLIGSHTPMAILPAGSANDLA
                     RTLKLPMEDIPRAVDAALNGATQRIDTIRVTTATGERHFLNVVGTGFDAEVAAAAERI
                     TVMRGAGRYVLAILRVLPRFKAATLNLRIDGEPRTERAMMIALANGPAYGGGMRVAPA
                     ARLDSGELEICIVGEVSKPEFLKAFPRVFRGTHVDHPAVTMLRGKRIEIEADHPLDLL
                     GDGERAGQLPARVEVHPQSLSIVTMDL"
     gene            4065..6644
                     /gene="pepN"
                     /locus_tag="VMZ33_05170"
     CDS             4065..6644
                     /gene="pepN"
                     /locus_tag="VMZ33_05170"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HUR16653.1"
                     /translation="MSYAPTLTAHARDILNETDATDRAARVPEAHYALSLDLAAGRPT
                     YRGDLTLRFAARGAGSMFLDFRGKTVELLDINGTSITPDWNGFRLTLPGDALGSDNTV
                     RIVYENDYDTTGDGFHRFVDPEDGEEYVYTNFEPYEAHRLFPCFDQPDIKGRYLVDVT
                     APSAWQVVANAPLEAETPASDDRTRHQFAESELFSTYLMALVGGPYVSRRITHNGLEL
                     GLYARRSMERYLNDQAPEIFEVTTQGLDFYAEMFEQPYPFAKYDQVFVPEYNSGAMEN
                     VGCVTYNEAYLFRDPATESQKLDRAETFLHELAHMWFGNLVTMRWWNDLWLNESFATY
                     ISYVAMTEATRFTNAWKVFNADIKRWAYQQDQLPTTHPIAGTAADTEIAFLNFDGITY
                     GKGASVLKQLAKYIGRDAFRDGLRLYFRRHGWSNATLADFLSSLQEKTSNDLEAWARL
                     WLSTASLNTLSTEWQSDGSQVSALAIQQTAPDEYPTLRPHALEVALARNGTGKLKIES
                     LAAWIDGPDTPIAEARGREIPDFVFPNYGDYAYAKVSLDEKSVDFIRHHLDKVDDALL
                     RQLLWMSLWEMVRDRELSSIEYLAIGRSELPDEPDHDILNSILDRTGLLMSRYVPESM
                     REAEAHQWYQVALAQVARSTGDKQLLWARSAIGVAATTDDVQQLARLATGAESLAGFE
                     LDQEMRWAVATKALAFDLPEADDMLETESARDRSDRGKRALLRAEAARPTAEAKEEAW
                     NRIHGDGYGSFHLTRAAMLGFWWPHQHELLEPYADRFFDRIEEVFDTRDHPFARAYLV
                     ALYPAYRAAPEVLARSRRMLDELDGSMPTLSRQLAESADDLDRQIKVREFAETGSNTP
                     VGG"
     gene            6646..>7218
                     /locus_tag="VMZ33_05175"
     CDS             6646..>7218
                     /locus_tag="VMZ33_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR16654.1"
                     /translation="MPGRLWVEPVTLEGSVVRLEPLSFDHADGLTAIGLDSEIWRWMP
                     SSFQTPGEMRGYIEKALAARAAGTDMPFTTIEAASGTIVGGTRYLNIEPQHRRVEIGY
                     TWIAPPWQRTAINTEAKLLMLSHAFNNLGALRVEFKTDSLNEQSRKALTGIGATEEGT
                     LRNHMLYESGRRRHSVYFSVIEEEWPRVRVH"
BASE COUNT         1272 a         2439 c         2333 g         1174 t
ORIGIN      
        1 cgtttacctg ggcgggaaca aattcacaag cctcggccgt gccggccgca cggaggagct
       61 cagccccggt gcccggatgc gcgccgatgc gatgttcgcc tcggccgtcc cagcgtggtg
      121 ccccgaggtc ttctgactga ggctggcacg gcgccgcatg acaacaaaac gcccgaagga
      181 gccgtccggc ccggtgaccg ctccactggc gaaccagtgg tgctgttccg gtttcgtggc
      241 cccttcggga cgaccacagg ttatgtctcg acccgccgcc atcgcaaggg cagttatcca
      301 cgagatgccg ccgcgacggc gggtctgtcc acgacctgtc cccaagcacc ccgtccacag
      361 cgccccaatc ggcgacgcca cttaaggttc gagggtgaac gacccgatcg acccggctta
      421 ctccggcggg ccgcagccgc ccgtcgcccg acccgccgtc gccatggccg ggcttctagt
      481 ggtgctgctc gtctttgtcg tcgggatggc tgtcggttcg agcgggatcc tcggccccag
      541 gtcgacccct agaccttcgc cggtcgctgt ccagcccacg ccggcgccgg gttccacgcc
      601 ggcaccgttg cccagcaacg tgcccggcaa cttcgcactg ttctggcagg cactgcagac
      661 ggtgcgcgac agcttcgtcg gccgtggtga gctcaccgac gagcagctca ccaacggtgc
      721 gatccgcgga atgatcgagg cgctcggaga caccggccac tccgtcttcc tgacgcccga
      781 agcagttgcg gctgagaacg aatccctcgg cggcaccgtc gtcggcatcg gcgtccttct
      841 cggcgagcgc aatgcgcagg tcgtcgtcgt gtccgtgatc tccggcggtc cggcggacga
      901 tgccggtatc cgctccggtg acaggatcct gactatcgac ggcgcttcgg tcgaaggcct
      961 cgcgccggag gaagtcgcac cgcgcgttcg cggcgaggcc gggtcgaccg tcgttctcaa
     1021 cattgagcga cccgccaccg gggaggatct ggagttcagc atcgtgcgcg aggagctgcg
     1081 gttcccggcc gcgtactgga cgatggtgcc gggcacgaat atcggcttct tgcggctcgt
     1141 ccagttctcg accggttctg ccgatgagct tcgcgctgcg cgcgatgagg caatagacgc
     1201 cggcgccgag tcgctcatcc tcgatttgcg cagcaacccc ggcggctacg tcgatgaggc
     1261 agtcgacatc gccagcctct tcctgagcga cagggttgtg ttcctcagcg aggacgcggc
     1321 gggcacgcgg acggaggtgc gcaccaattc gtcggtcgag gcgacagatc tgccactcgt
     1381 tctcctgatc gacgagggca gtgccagttc gtctgagatc gtcaccggcg cgatccgctc
     1441 cgccggcagg gcagagctga tcggcacgac aacgttcggt acggggaccg tgctgcagac
     1501 gttccccctc accaacggct ccgccattcg actcgccacc gagcgctggc tgacgcccga
     1561 cggcgagctg atcttcggcc aggggatcga cccgaccacc gcggttgagc taccggcgga
     1621 ccagacgccg ctggacccaa tcgatgtacg ggacatgccc gcggacgaga tcgacagcct
     1681 tcccgacgat cagctcagga gggcaatcga gctcctcacc cagtaggcgc gcggccgcgt
     1741 gatcgctagc catccgaaac cccgcggcgg cttgacgcag cgctggtgcg ctgtcggcgg
     1801 gtcgaacatc gggtcatggt tgaaatcgga tatgcgctct cgagcgaaga acacacacca
     1861 ctcgacatgg tccgctgggc tgagagggct gaacaggcgg gcttcggatt cgccctgatc
     1921 tctgaccact atcacccgtg gatcgaccgg cagggccaca gcgggttcgt gtgggggctt
     1981 atcggcgcga tcagtcagcg aactgagaag ctggtcctcg gaacgggcgt gactgcgccc
     2041 atcatccgga cgcaccccgc gatcatcgcg caagccgcag ccacggccgc gtgcctgatg
     2101 cccgggcgct tcttcctcgg cgtcggatca ggcgagaacc tgaacgagca catcctgggc
     2161 gacaaatggc cgcccgcgga tatccgactg gagatgctcg aggaatcgat cgacgtcatg
     2221 cgcacgctct ggcagggcgg cgtgcaggac tacgacggta gctactacgt ggtcgagaac
     2281 gcgcgcgtgt acgacctccc cgatgacgag atccaggtca tggtcgccgc atcaggtgag
     2341 aaagcctcac agctcgccgg gcgggtcggc gatggtctta tcggaacgtc ggctagcact
     2401 gacaccgtta ctgcgtttga aaaggcgggc ggttcaggca agccgcgcta cgggcagatg
     2461 agcgtctgct gggctgagac ggaggaagag gccgtccgcg tcgctcacga ggtctggccc
     2521 atcgccggta tgggcggcca gctgggccag gagctcgcgt tgcctgcgtt atacgaggcc
     2581 gttgccgagc tggtgacgcc tgaccagatc aaggagtcga ttgtctgcgg ccccgacgct
     2641 gagcccgtgc gcaagaagat ccaggagtac gccgacgccg gatatacgca tgtctatctg
     2701 catcagatcg ggcccgacca ggaaggtttc ttccgcttcg cggagcgcga gctactgcgt
     2761 gagccggtgg ccgccggcct tgcgggcgcg cgctagcgcg tgctgaggtc gcgcaacacc
     2821 gagtagagcg cgacggcaac cccgagcggc gcagcccagg caactccggc cagaaagata
     2881 ttcatgaact ggagccggcc gtcggcgctg aagagagacg tcaacgcgat cgtggcccag
     2941 accagcgacc agaagcccgc gccgatcaca aacaggttgt gagcccgcgc tgcccgcatg
     3001 cattgaatcg tacgactgcc agaagctcgc ggcacgcgcg aataacatcg gcgcgagatg
     3061 acagaacggt cgaagcgatc cagggtcgcg ctgatcgtca atccggttgc aggtggcggg
     3121 cggggggcgc gcgtcgtcgg tgaggccgta tacgagctgc gcaagcgtgg ggtcgagccg
     3181 cgcatcatcg tgtccgtctc gcccgacgag ccgccgggtg acgcccgacg agcggcgaac
     3241 gacggcgccg atctcgtcat cgccgtgggt ggcgacggtc atgccgccgc cgtcgcgtcg
     3301 ggactcatcg gcagccatac gccgatggcg atcctgcccg ccgggagcgc caatgacctg
     3361 gcccgcacgc tcaagctgcc aatggaggac ataccgcggg ccgtcgacgc cgccctcaac
     3421 ggggccactc aacgcatcga cacgatccgc gtcacgaccg ccactggtga gcgccatttc
     3481 ctgaacgtcg tcggcaccgg tttcgacgcc gaggtggcgg ccgcagccga gcgcatcacc
     3541 gtcatgcgcg gcgccggtcg ctacgtcctg gccatcctgc gtgtcctgcc gcgcttcaaa
     3601 gcggccacgc tcaacctgcg aatcgacgga gagccccgca ccgagcgcgc aatgatgatc
     3661 gcgcttgcca acggtccggc gtatggcggt gggatgcgcg ttgccccagc tgccaggctc
     3721 gatagcggcg aactcgagat ctgcatcgtc ggcgaggtga gcaagcctga gttcctgaag
     3781 gcctttccgc gggtctttcg cggcactcac gtcgaccacc ctgccgtgac tatgctccga
     3841 ggcaagcgca tcgaaatcga ggccgatcac ccgctggatc tacttggcga cggcgagcgc
     3901 gctggccagc tcccggcacg ggtggaagtg catccgcagt cgctttcgat cgtcaccatg
     3961 gatttgtaat ggaaggtgac tagcgcagac accccgagtc ggggttacac tcccgcgtgc
     4021 ccgccgtttg gccgtggccc agagcgggag ctgcgaggaa aaagttgtct tacgccccga
     4081 ccctgaccgc ccacgcgcgc gacatcctga acgagacaga cgccacggat cgcgccgccc
     4141 gtgtgcccga agcacactac gcgctcagcc ttgacctggc cgccggccgc ccgacgtacc
     4201 gcggcgacct gacgctccgc ttcgcggctc ggggcgcggg cagcatgttc ctcgactttc
     4261 gtggcaagac cgtcgagctt ctcgacatca acggcacgtc catcacgccc gattggaacg
     4321 gcttccggct gacgttaccc ggtgacgcgc tcgggtcgga caacacggtg cgcatcgtct
     4381 acgagaacga ctacgacacg accggggacg gcttccaccg attcgtcgat ccggaggatg
     4441 gcgaggagta cgtctacacg aacttcgagc catacgaggc tcaccgcctt ttcccgtgtt
     4501 tcgaccagcc tgacatcaag ggtcgctacc tcgtcgatgt gaccgccccg tcagcgtggc
     4561 aggtcgtggc caacgccccg ctggaggcgg aaacaccggc gtcggacgac cggacgcgtc
     4621 atcagttcgc tgagagcgag ctcttcagca cttacctgat ggcgctcgtc ggcggcccat
     4681 acgtgtctcg ccgcatcacg cacaacggcc tcgagctggg cctctatgcg cgccgctcga
     4741 tggagcgcta tctcaacgac caggcgcccg agatcttcga ggtcacaacc cagggcctcg
     4801 acttctacgc cgagatgttc gaacagccct accccttcgc caagtacgac caggtcttcg
     4861 tgcctgagta caactcgggc gcgatggaga acgtcggctg cgtcacatac aacgaggcgt
     4921 acctcttccg cgacccggcg acagagagcc agaagctcga tcgcgccgag acgttcctgc
     4981 acgagcttgc ccacatgtgg ttcggcaatc tcgtcacgat gcgctggtgg aacgacctgt
     5041 ggctcaacga gagcttcgcc acgtacatca gctacgtggc catgacggag gccacgcgct
     5101 tcaccaacgc ctggaaggtc ttcaacgctg acatcaagcg ctgggcctat cagcaggacc
     5161 agctgccgac cacgcacccg atcgccggca ccgcggcgga caccgagatc gcgttcctga
     5221 acttcgacgg gatcacatac ggcaagggcg catcggttct caagcagctg gccaagtaca
     5281 tcggccgcga cgcattccgt gatggcctgc ggctgtactt ccgccgccat ggctggtcga
     5341 acgccactct ggccgacttc ctgtcgagtc tccaggagaa gaccagcaac gacctcgagg
     5401 cgtgggcgcg cctgtggttg agcaccgctt cgctcaacac gctctccacc gagtggcagt
     5461 ccgacggctc gcaggtgtcc gcgcttgcca tccagcagac ggctccggac gagtacccga
     5521 ccctgcgccc gcacgccctc gaggtcgctc tggcgcgcaa cggaacgggc aagctcaaga
     5581 tcgagtcgct cgcggcgtgg atcgacgggc ccgacacgcc gattgccgag gcgcgcggac
     5641 gagagatccc ggacttcgtt ttcccgaact acggcgatta cgcatacgcc aaggtttcgc
     5701 tcgacgaaaa gtcggtcgat ttcatccgcc atcacctcga caaggtcgac gacgcgctgc
     5761 tccggcaact gttgtggatg tctctgtggg agatggtccg cgaccgcgag ctgagctcga
     5821 tcgagtacct ggccatcggc cgcagcgagc tgcccgacga gcctgatcac gacatcctga
     5881 actcgatcct cgatcggacc gggctgctca tgagccgcta cgtgccggag tccatgcgcg
     5941 aggcggaggc tcatcagtgg tatcaggtcg cgctggcgca ggttgccagg tcgaccggcg
     6001 acaagcagct cctctgggcg cgttcggcaa tcggagtcgc cgcgacgacg gacgatgtcc
     6061 agcagctggc gcggctggcg acgggcgctg agtcgctcgc cggcttcgag ctcgaccagg
     6121 agatgcgctg ggctgttgcc accaaggcgc tggccttcga cctccccgag gccgacgaca
     6181 tgctcgagac cgaatcggct cgcgaccggt ccgaccgcgg caagcgtgcc ctactgcgcg
     6241 ccgaagccgc acgaccgacc gccgaggcca aggaagaggc ctggaatcgc attcacggcg
     6301 acggatatgg ctcgttccac ctgacccgcg cggcgatgct cggcttctgg tggccgcatc
     6361 agcacgaatt gctggagccg tacgccgatc gtttcttcga tcgtatcgag gaagtgttcg
     6421 acacccgcga ccaccccttt gcgcgggcct acctggtcgc cctctatccg gcctaccgcg
     6481 cggcgcccga agtcctggcg cgatcccgcc ggatgctcga cgagctggat ggctcaatgc
     6541 cgaccctgtc acgccagctg gccgaatcgg ccgacgacct cgaccgccag atcaaggttc
     6601 gcgagttcgc cgagaccggc agcaacactc ccgtcggcgg ctgacgtgcc cggccggcta
     6661 tgggttgagc cggtaactct cgaaggatcg gtagtccgtc tcgaaccgct ttcgttcgac
     6721 cacgccgacg gtctcaccgc tatcgggctc gactcggaaa tctggcgctg gatgccatcg
     6781 tcgttccaga cgccgggcga gatgcgtggc tacatcgaaa aggcgctcgc cgctcgcgca
     6841 gcaggaacgg acatgccgtt cacgacgatc gaggccgcgt cgggcacgat cgtcggcggt
     6901 acccgctatc tgaacatcga accgcagcat cgtcgggtgg agatcggcta cacgtggatc
     6961 gcaccgccgt ggcagcgcac cgcgatcaac actgaggcca agctcctcat gttgtcccac
     7021 gcgttcaaca atttgggcgc gttgcgagtc gagttcaaga ccgactccct gaacgagcag
     7081 tcacggaagg cgctgaccgg gatcggcgcg acggaggagg ggacgctccg caaccacatg
     7141 ctgtacgaga gcggccggcg ccggcactcg gtgtacttca gcgtgatcga ggaggagtgg
     7201 ccacgcgtcc gggtgcac
//
LOCUS       DATLCO010000213         3172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6875834, whole genome shotgun sequence.
ACCESSION   DATLCO010000213 DATLCO010000000
VERSION     DATLCO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6875834"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1159)
                     /locus_tag="VMZ33_05180"
     CDS             complement(17..1159)
                     /locus_tag="VMZ33_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16655.1"
                     /translation="MSDEGRGATTAGLIRCNQCSHEVPRLEYCVRCGDPLSDEYNVES
                     LKERKGRFAAAPDESARTVALISTLFPQLPRAETRAFRLSLIIGVAIVVGLALLGYFP
                     VALVAAAVLVPLLMLIYLWVIDIYEDQPLWVLGATITWGAATGIVTGLVLRNLPGSGF
                     GVGGPSLETIAVSGIVVPLFEGALMLTGALLMLPLRRFNDVLDGATFGAAAAISFSGA
                     HVIVQSLPILRAGLRPQTDPVQSAIQLVSLGVLQPVVAAGAIGAVGAAVWLRYRAPVN
                     DRAALGVVGIPVMAVLGAAVLLVAAGLAKATLPLVPQTVVLIVLAAVALVWLRRVIHL
                     GLLQESREIEIGSEIVCPNCGRPTPSHTFCGKCGVSLSALPRSRGR"
     gene            complement(1160..1453)
                     /locus_tag="VMZ33_05185"
     CDS             complement(1160..1453)
                     /locus_tag="VMZ33_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16656.1"
                     /translation="MSSNIAQSYPYSSETEAERVATLERLTGQFEGLRDKIVAEAEPL
                     PEPEYPDEPGRPHRQWIWVCPKHVEGRLHVAGHARERHALFAVCDVEGETYLR"
     gene            complement(1555..>3172)
                     /locus_tag="VMZ33_05190"
     CDS             complement(1555..>3172)
                     /locus_tag="VMZ33_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HUR16657.1"
                     /translation="LPQVVKSARVMKAAVGYLIPYIEAEKAALLAAGGGNGHDSGAAE
                     PRGAGTVVMATVKGDVHDIGKNIVGVVLGCNGYRIVDLGVMVPWPRILETARLENADA
                     IGLSGLITPSLEEMRTVAQEMERAGLTLPLLIGGATTSRAHTAVRLEPAYTGPVIHVQ
                     DASRAVGVVRSLLDEGARGGFVAETRRQYEELRRQFADRDDRTKRLAIDEARANRFTI
                     DWTASGAPPRPGFLGGRSFRNVDLAELVDYIDWTPFFAAWEMPGHYPEILSDARMGEA
                     ARSLYDDARKLLARVVDERLLSASASVGFWPAASTPDDDIVLYADESRTAELERLHAL
                     RQQMAKPDGRPNIALSDYTAPANSNVEDYVGAFAVTAGRGLEDAKKAFEQAGDDYSAI
                     LLTSLADRLAEASAEWLHAKVRRELWGYAPDEALDGDALIREEYSGIRPAPGYPAQPD
                     HTEKRTIFRLLDAEREAGIRLTESMAMIPGSSVSGLYFWHPEARYFGLGRIGRDQLVD
                     YARRKGWSIAEAERWLALNVAEEKATPAAA"
BASE COUNT          546 a         1084 c         1046 g          496 t
ORIGIN      
        1 ggtggctgct ggccgctcat cgaccgcgac ttctgggcag cgcgctgagc gacacgccgc
       61 acttcccgca gaaggtgtgc gacggggtcg ggcggccaca gttcgggcag acgatctcgg
      121 agccgatctc gatctcgcgc gactcctgca gcagacccag atggattact cgtctaagcc
      181 agacgagcgc gactgcagcc agcacgatca ggaccacggt ttgaggcacg agcggcaagg
      241 ttgccttggc caggccggcc gcgaccagca ggaccgcggc accgagcacc gccatcacgg
      301 ggatgccgac gactcccagc gcggcgcggt cgttgaccgg ggcgcgataa cgcagccaga
      361 ccgcggcacc aaccgcgccg atcgccccgg ccgcgacaac cggctgcaga accccgagcg
      421 aaacgagctg gatggccgat tgcacagggt ccgtctgtgg ccgaagacct gcacggagga
      481 tgggcagcga ctgcacgatg acatgggccc cactgaagct gatcgccgcg gccgcgccga
      541 acgtggcgcc gtcgaggacg tcgttgaagc ggcgcaacgg cagcatcagg agcgcacccg
      601 tgagcatcaa tgctccttcg aacagcggca cgacgatgcc tgagactgca atcgtttcca
      661 gcgatggacc accgacgccg aacccgctac cgggcaggtt gcgcagtacc aggccggtga
      721 cgatgcctgt cgcagcaccc caggtgatcg tggcccccag aacccacagg ggctggtcct
      781 cgtagatgtc aatgacccac aggtagatga gcatcaacag cgggacgagc acagccgcgg
      841 caacgagtgc gacggggaag tagccgagca acgccaggcc gacaacgatg gcaacgccga
      901 tgatcaagga cagccgaaac gcgcgcgtct cggcacgtgg cagttgcggg aagagagtgc
      961 tgatgagcgc gacggtccgt gccgattcat cgggcgccgc cgcgaatcga cccttccttt
     1021 ccttgagtga ctcgacgttg tactcgtcgc tcaagggatc gccgcaacgg acgcagtatt
     1081 cgaggcgcgg cacctcgtgg ctgcactggt tgcagcgaat caggccggca gtcgttgccc
     1141 cccgcccctc gtcactcatc tagcgcaggt aagtctctcc ttcgacgtcg cacacggcaa
     1201 acagggcgtg tcgctcgcgg gcgtggccag cgacgtgtag ccggccttcg acatgcttgg
     1261 ggcagaccca gatccactgt cgatgcggcc ggcccggctc gtctgggtac tccggctccg
     1321 gcaggggctc tgcctcagcc acgatcttgt cgcgaagacc ctcgaactga cccgtcaggc
     1381 gttccagcgt cgcgacacgc tcggcctcgg tctcgctcga gtaggggtag ctctgggcga
     1441 tgttcgacga cacagtgtgc tccgacaagg gaatggccag ccgcggaggt cgttgagcgc
     1501 ggcgcgaccg gccggtccgg agtatatcgt gccgatttgg ccctcaaagg gccatcaggc
     1561 ggcggctggc gtggccttct cctcggcgac gttcagggcc agccaacgtt ccgcttcggc
     1621 gattgaccag cccttccggc gcgcatagtc gaccagctgg tcgcgcccga ttcgtccaag
     1681 tccgaagtag cgtgcctccg ggtgccagaa gtagaggccg ctcactgacg aaccggggat
     1741 catcgccatc gactcggtca gcctgatccc ggcctcgcgc tcggcatcga gcaggcggaa
     1801 gatcgtccgc ttctccgtgt gatcgggctg cgcgggatac ccgggggccg gccggattcc
     1861 gctgtactcc tcgcggatca gcgcgtcgcc gtcgagcgcc tcgtcgggcg catagcccca
     1921 cagctcccgg cgaaccttcg cgtgtagcca ttcggccgag gcctccgcca ggcgatcggc
     1981 cagcgatgtg agcaggatcg ccgaatagtc gtcgccggcc tgctcaaacg ccttcttggc
     2041 gtcctcgagc ccgcgccccg cggtgaccgc gaaggcaccc acgtagtcct cgacgttcga
     2101 gttcgcaggg gcggtgtaat ccgacaacgc gatgttcggt cgaccatccg gtttcgccat
     2161 ctgctgccga agtgcgtgga ggcgctcgag ctcggccgtc cgcgactcgt cggcgtacag
     2221 gacgatgtcg tcgtcgggtg tcgacgccgc cggccagaag ccaaccgagg ctgaggctga
     2281 cagcaaccgc tcgtccacca cgcgtgccag cagcttccgt gcgtcgtcgt acagggaacg
     2341 cgccgcctcg cccatccgcg catcggacag gatctcgggg tagtgcccgg gcatctccca
     2401 ggcggcaaag aaaggcgtcc agtcgatgta gtcgacgagc tcggcgaggt ccacgttcct
     2461 gaacgagcgg cccccaagga aaccgggtcg cggaggtgcg ccgctggcgg tccaatcgat
     2521 ggtgaagcgg ttcgcgcgtg cttcatctat cgccagccgc ttcgtccggt cgtcacgatc
     2581 ggcgaactgg cgccgcagct cctcgtactg gcgtcgggtc tcggcgacga agccaccgcg
     2641 cgcgccttcg tcgagcaatg accgcacgac gcccaccgcg cgcgacgcat cctgcacgtg
     2701 gataacgggg cccgtgtagg caggttcgag gcgcaccgcc gtatgggcac gcgacgtcgt
     2761 ggcgccgccg atcaacaacg gcagcgtcag gccggcccgc tccatttcct gggcaacggt
     2821 tcgcatctcc tcgagggatg gagtgatcag gcccgacagc ccgatcgcgt cggcattctc
     2881 cagtcgcgcc gtttcgagga tgcgcggcca cggcaccatc acgcccaggt cgacgatgcg
     2941 gtagccattg cagccgagca cgactcccac gatgttcttg ccgatgtcgt ggacgtcgcc
     3001 cttgaccgtg gccatgacca cggttccggc accacgaggc tcggctgcac cgctgtcgtg
     3061 accattgccg ccaccggcgg caagcagcgc ggccttctct gcctctatgt acgggatgag
     3121 gtagccgacg gccgccttca tcacgcgggc actcttcacg acctgcggca gg
//
LOCUS       DATLCO010000214         3910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9276983, whole genome shotgun sequence.
ACCESSION   DATLCO010000214 DATLCO010000000
VERSION     DATLCO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9276983"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VMZ33_05195"
     CDS             <1..654
                     /locus_tag="VMZ33_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16658.1"
                     /translation="LIRIVVADDHPMFRAGVVASLSAQPDVEVVGEGASADEAVRLAH
                     EHNPDVCLLDITMPGGGLNAAREITAGVPGSKVVMLTVSEDEDDLLAAMKAGASGYVL
                     KGAAAGELVSVLRSVNAGEVYVAPGLAWGLLREMSRPRSTPILDELSTREREVLELVA
                     AGLSNQEIGDRLHLAEKTIKHYMTSILGKLRVRSRVEAALLAYREGITPPMPAEVDD"
     gene            complement(709..1059)
                     /locus_tag="VMZ33_05200"
     CDS             complement(709..1059)
                     /locus_tag="VMZ33_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HUR16659.1"
                     /translation="MTNGECLFCRIAAGEIPAEIVLQDDLVLAFADINPKAPTHLLLI
                     PRKHIESAKDLTEEDGPMLGRLFAVAARLAGEAGISEHGFRLVTNSGAGAGQSVFHIH
                     FHLLGGRQLGWPPG"
     gene            complement(1087..1542)
                     /locus_tag="VMZ33_05205"
     CDS             complement(1087..1542)
                     /locus_tag="VMZ33_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16660.1"
                     /translation="MPVLIAIHVTLAISLFLPSILLPFALRLRGGQWREQQGSVSRAL
                     FWLQRNGTLVIGAGLAVTGVAMLTILGPQLLAQPWLFIALLIYATNIVLAFFVQRPAL
                     RGLIGMRPLTSDAERDRWKLWARRQRYVSYVMATLVGLIAFLMSTKPQF"
     gene            complement(1558..2577)
                     /gene="prmA"
                     /locus_tag="VMZ33_05210"
     CDS             complement(1558..2577)
                     /gene="prmA"
                     /locus_tag="VMZ33_05210"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HUR16661.1"
                     /translation="MEASASSGSQERDSGPDPGCDQLTVADGTSAAGSWIELAVSADQ
                     EAVEQVSEILSRSCPGGVSVDAPFEVLEDGLAARVDSTRPAILRAYVSALDPQVSRAA
                     IEQVRRDLGHLQAFALRPIGELETRVVHDEDWADAWREHFPVMRVGRRVVIQPTWREF
                     EAADEDVVIRLDPGMAFGTGLHPTTRLCLAGIERWGDAGLVEGRTVLDVGCGSGILAI
                     LCGLLGASEIMAVDTDPLAIETSIANAALNDVGLVARQGSVPLAEKREFDLVIGNLIA
                     GVVIDLAPALAASVAPAGGRLLASGIFHEREAEVRTAFASAGLMIVGSRTDGDWIALE
                     AQRVG"
     gene            complement(2508..3752)
                     /gene="dnaJ"
                     /locus_tag="VMZ33_05215"
     CDS             complement(2508..3752)
                     /gene="dnaJ"
                     /locus_tag="VMZ33_05215"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HUR16662.1"
                     /translation="MATQRDYYDILGVPRGAGDDEIKKSFRKLAQQWHPDVNTSTEAD
                     VRFKEINEAYQVLSDPQRRQAYDMFGHAATGAAAGGGFDPFGTGGSASGGGFQGFGDL
                     FDTLFGAASGGARRPRVPTGADLRYDLRLTFAESINGAEKEIDFGSLARCDTCSGSGA
                     EPGTSPTTCLKCTGTGELRNVRQTMLGQMVSVTPCDRCHGTGKIVESPCHTCHGEGRV
                     ERRRKLRVTVPAGIDDGHQIRLSGEGEAAPRGGVPGNLYVVAHVAAHPELRRQETELF
                     YDLSLSITQAALGARIRVPTADAEEEVEIKPGTQAGTEIRLRGKGVPHLRRQGSRGDV
                     HVLVDVHVPNRLTARQRALLEELAGEFGEVDDEPEATGASEAGAGSGGQTDAPTGAAP
                     SKRARRRAHKNGIRDRIRDVIS"
BASE COUNT          679 a         1332 c         1267 g          632 t
ORIGIN      
        1 ctcatccgta tcgtcgtcgc ggacgatcac ccgatgttcc gcgcgggcgt cgtggcgtcg
       61 ctgtcggcac agccggacgt cgaggtcgtg ggtgagggtg catcagccga cgaagccgtc
      121 cgtctcgcgc acgagcacaa ccctgatgtg tgcctactcg acatcacgat gcccggtggc
      181 gggctcaacg ccgcacgcga gatcaccgcc ggcgtacccg gatcgaaggt cgtgatgctc
      241 accgtttctg aggacgagga cgacctgctc gcggcgatga aggctggcgc atccgggtac
      301 gtgttgaagg gcgcggccgc cggggagctc gtttcagtcc tgcgctcggt gaacgccggc
      361 gaggtctacg tcgcgccggg tctcgcgtgg ggtcttctgc gcgagatgag tcggccccgc
      421 tccacgccca tcctcgacga gctgtccacg cgcgagcgcg aggtgctgga gcttgttgcg
      481 gcgggtttga gcaaccagga gatcggggat cggttgcacc tcgccgagaa gacgatcaag
      541 cactacatga cgagcatttt gggcaagctc cgcgtgaggt cgcgcgtcga agctgcgctc
      601 cttgcctacc gcgaaggcat cacaccaccg atgcccgccg aggtcgacga ctagcccgac
      661 tcgattccag atccgatccg atgtgcgcct gacgcacatc cgcggcgatc agccgggtgg
      721 ccagcccagc tgtcgaccac cgagcaggtg gaagtgaatg tggaacacgc tttggccggc
      781 gcccgcacct gagttcgtca cgagccggaa gccatgctca ctgatgcccg cttccccggc
      841 aaggcgcgcg gccactgcga acagccggcc gagcattggt ccgtcctcct cagtgaggtc
      901 cttcgcggac tcgatgtgct tccgggggat caggagcagg tgcgttggcg ccttggggtt
      961 gatgtccgca aaggccagga ccagatcgtc ctggaggacg atctcggcgg gtatctcacc
     1021 agccgcgata cggcagaaaa ggcattcccc gttggtcatc ggcggatggt agcgacgacg
     1081 cttggctcag aactgcggct tggtcgacat caggaacgcg atcagcccca cgagcgtggc
     1141 catcacgtag ctcacgtagc gctgccggcg tgcccacaac ttccagcgat cccgctcggc
     1201 atcgctcgtg agcggcctca tgccgatcaa cccccgcagc gcaggccgct gcacgaagaa
     1261 cgccaggact atgttcgtgg catagatcaa cagggcgatg aagagccacg gctgcgcgag
     1321 gagttgcgga ccgaggatcg tgagcatcgc gacacccgtg actgccaggc cagccccgat
     1381 caccaatgtg ccatttcgtt gcagccagaa aagcgcccga gagacgcttc cctgctgctc
     1441 ccgccactgc ccgccacgca accgaagcgc gaatggcagc aggatgctcg gaaggaacaa
     1501 cgagatcgcc agagtgacgt ggatggcgat gaggaccggc aagaaggacc ccacgagtca
     1561 gccaacccgc tgcgcttcga gggcgatcca gtcgccgtct gttcggctgc cgacgatcat
     1621 caatcccgcc gaagcgaacg ccgtccggac ctcagcctca cgctcgtgga atatgccgct
     1681 cgccagtaga cgaccgccag ctggcgcgac tgaggctgcc agggcgggag ccaggtcgat
     1741 cacgacgccg gcgatcaggt tgccgatgac gaggtcgaac tcgcgcttct cagccaacgg
     1801 caccgatccc tgccgcgcaa ccagcccaac gtcgttcagt gccgcattgg cgatcgaggt
     1861 ctcaatggcc agcgggtccg tatcgacagc catgatctcc gaggctccga ggagaccgca
     1921 aaggatcgcc aggatcccgg acccacaccc gacgtcgagc acggtccggc cttcaaccaa
     1981 accggcgtcg ccccagcgct cgatgccggc caggcacagc cgcgtggtcg gatgtagccc
     2041 ggtgccgaac gccatcccgg ggtcgagccg gatgaccacg tcttcgtcgg ccgcctcgaa
     2101 ttcgcgccac gttggctgga tgacgacacg ccgtccgaca cgcatgacgg gaaagtgctc
     2161 acgccacgcg tctgcccagt cttcgtcgtg aacgacgcgc gtctcaagct ccccgatagg
     2221 ccgcaatgcg aatgcctgga ggtggccgag atcgcgccgc acctgttcga tcgccgcccg
     2281 ggacacctgc gggtccaaag ccgacacgta cgcgcgcagg atcgcgggcc gagtcgaatc
     2341 gacccgcgcc gccaggccat cctcgagtac ctcaaacggg gcgtcgaccg acacaccgcc
     2401 cggacacgag cgcgacagga tctcgcttac ctgctcaacc gcctcctgat cggcgctgac
     2461 tgcgagttcg atccaacttc ccgcggccga tgttccgtcc gcgacggtca gctgatcaca
     2521 tcccggatcc ggtcccgaat cccgttcttg tgagcccgac gacgcgctcg cttcgatgga
     2581 gcggcgccgg tcggggcatc agtctggccg ccagaacccg cccccgcttc ggacgcgccg
     2641 gtcgcctcag gctcgtcatc gacctcaccg aactctccgg cgagctcttc gagcagggcc
     2701 cgctgccggg cagtcagccg attcgggacg tggacgtcga cgaggacatg gacgtcgcca
     2761 cgcgaaccct gtcgacgcag atgcggcacg cccttgccgc gcagtcggat ctccgtccct
     2821 gcctgggtcc ccggtttgat ctcgacttcc tcttcggcgt cggcggtcgg cacgcgtatg
     2881 cgggcgccca gggcggcctg cgtgatcgag agggaaaggt cgtagaagag ctccgtctcc
     2941 tggcggcgca gctcgggatg tgcggcgacg tgcgccacca catagaggtt gccgggcacg
     3001 ccgccacgtg gcgccgcctc accctcgccg gagaggcgga tctgatgacc gtcgtcgatg
     3061 cctgcaggaa cggttacgcg gagcttccgt cgtcgctcaa cgcgtccctc cccatggcag
     3121 gtgtggcacg gcgattcgac gatcttgccc gtcccgtggc agcgatcgca cggcgtgacc
     3181 gagaccattt gtccgagcat cgtctggcgc acgttgcgca gctcgcccgt gcccgtgcac
     3241 ttgagacatg tggttggtga cgtccccggc tcagctccgg acccagagca ggtatcgcag
     3301 cgcgccaggc tgccgaaatc gatctccttc tccgccccgt tgatggactc ggcgaaggtc
     3361 agccgcaggt cgtagcgcag atcggcaccg gttgggacac gcggtcgccg tgcccctccg
     3421 ctggccgcgc caaacaacgt gtcaaagagg tccccgaagc cctggaaacc gccgcccgag
     3481 gcggagccac cggtgccgaa cggatcaaac ccgccgccag ccgctgcccc tgtcgcagcg
     3541 tggccgaaca tgtcgtaagc ctgccgacgc tgtgggtcgg acagaacctg gtaggcctcg
     3601 ttgatctcct tgaagcggac gtcggcttcg gtcgaggtat taacgtcggg gtgccactgc
     3661 tgggctagct ttcgaaatga cttcttgatt tcgtcgtcgc cggcgccgcg gggcacgccc
     3721 aaaatgtcgt agtaatcacg ttgggtggcc atgcgtgatc gagtatacgg agggtcgagg
     3781 ggatgaccga tcgaggcatg gtcccgttgc gccgcacgtg accgcggcgc tcagtctcac
     3841 ggccgaggaa gccaggcgct tcctcatcgc gcgtcatggg ctggcgccgc cgcgcgcgct
     3901 gccagcggaa
//
LOCUS       DATLCO010000215         4564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5560872, whole genome shotgun sequence.
ACCESSION   DATLCO010000215 DATLCO010000000
VERSION     DATLCO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5560872"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VMZ33_05220"
     CDS             complement(<1..533)
                     /locus_tag="VMZ33_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HUR16663.1"
                     /translation="MTTAEMGNMLEDPAAIRRRDPGGMLSHIANFGAQMRTGWEISRS
                     LQLGDLHRGASSVVVLGMGGSAVCADLARAIFADRLTVPIVSVRDYELPAWVGRTTFV
                     VAISHSGATEETIAALGSALERRCPVAVITTGGPIGDVAARVGLPRLVFPNETPPRAS
                     LGYTLACLRGCSNVRGCS"
     gene            complement(535..2022)
                     /locus_tag="VMZ33_05225"
     CDS             complement(535..2022)
                     /locus_tag="VMZ33_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HUR16664.1"
                     /translation="MTDGSASAPLPPIVFGTDGWRARIGEDYTYENVRRCAQGVAEWA
                     RSAGTSQKGVVIAYDRRFASEFFAQAAAEVLLAYDIPVAMSQNAVPTQMTSYEVVERG
                     AACGIMITASHNPYPDNGFKVKSPSGAAASPDMLAVIEKTIRDYAGSQPPRQAYAEAV
                     ASGKVELYDPYPGYVEYTRRSLDLDRLKAADMRVLVEPMWGAGAGWISRLLAGGRIQI
                     TEIHQERNPFFGGVNPEPIRPNIDESLSLLAAGGYDLGLLLDGDADRAGAADEKGTFI
                     HQLQVMGLLMYYMVEHRGLRQPVVSTINETSMVGRLGEKYGVDVFETPVGFKYVGPKM
                     IETGAMMGGEESGGYGFGMHLPERDGIYADLLLLDLFIREREAGRWPVSAAVEHLHSI
                     AGQSFYLRSDVHVDRQAYPALKDRLLVELVAQAPTSVAGHPVIRTDELSTKDGFKFWL
                     DDGSWLLVRFSGTEPLVRVYAEATSADLRDGILAEGSALVRGEAG"
     gene            complement(2019..3083)
                     /locus_tag="VMZ33_05230"
     CDS             complement(2019..3083)
                     /locus_tag="VMZ33_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HUR16665.1"
                     /translation="MYAVVLAGGGGTRLWPLSRRARPKPFLPLLGAESLFQLTLRRIE
                     PLIDGAATIVVSEQRHLPLVEQQAPQIPSRQRLGEPFGRNTAAAVALAAILSGRRDED
                     VMVVLPADHFIADEEGFRRTLGVAAQAAQDGSLVTLGIEPTGPETGYGYIVGASADEA
                     EVGARRVERFVEKPPRERALELLASPNGAWWNAGIFVWRHDALLAGLAKYAPQIGNGI
                     RRGLDTGKSLTSIYEELPDISIDRALLEPASVEGKVKVVPADVGWSDLGSWDALHRAL
                     AARTRERHGVVAVGRSESVASSNVLVHSTGGRLVVTIGLADTIVVDTTDVVLVCAADK
                     AQEVRAIVERLTQAKETEHL"
     gene            complement(3128..3718)
                     /locus_tag="VMZ33_05235"
     CDS             complement(3128..3718)
                     /locus_tag="VMZ33_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HUR16666.1"
                     /translation="MTTRARHQSAPVEDTDLDLAAQLRLQRRARGMTLRDVAGRAGVT
                     ESFLSQVERGVASPSVASLQRIARGLGLSIAELFAGPATAGRVVRRDERRHVTYAGLG
                     AVDEFLTPPTATRLQVILSTIEPGGGTGDEPYTHESDEEVVLVLEGELDLWVADEHYH
                     LQEGDTVTHSSRLPHRNRNGGSKPARVLFSITPPSF"
     gene            3864..>4564
                     /locus_tag="VMZ33_05240"
     CDS             3864..>4564
                     /locus_tag="VMZ33_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR16667.1"
                     /translation="MHAAPAVLRSAALFSATALLVVACAGSGSSPAPSAAPSTAPSIG
                     PSAGPSASPSSAPSAAESKAPTAGPTEGAVTGSLSVLEWSGYEDPLYWADFAEKYPDV
                     EVNFDFGISDADIYGKMKAGSQSDLFHAYTGWLQFYVDEGLVQEIDTTKLANWDKVPD
                     HFKQVGQIDGKQYFLPFDWGFTSILYNTEHVDEVTSWSALFDETYDNHVSMWDDGPGA
                     VTVSSYIHDYDETAVT"
BASE COUNT          768 a         1540 c         1488 g          768 t
ORIGIN      
        1 gagcatcccc gcacgttcga gcagccccgc aaacaggcta gcgtgtaacc gagggacgcg
       61 cgtggcggcg tctcgttcgg gaataccagc cgtggaagtc cgacgcgcgc ggcgacgtcg
      121 ccgatcggcc ctccggtcgt gatcactgcg acggggcagc gcctctccaa cgccgacccg
      181 agcgcagcaa tcgtctcctc ggtcgccccg ctgtgcgaga tcgcgactac gaacgtggtc
      241 cggccgaccc acgccggaag ctcgtaatca cggaccgaca cgatgggaac ggtcaggcgg
      301 tcggcgaaga ttgctcgggc gagatccgcg cacacggccg agccgcccat ccccaggaca
      361 acgacggacg aagcgccgcg gtgcaggtca ccgagctgca gggatcggct gatctcccag
      421 ccagtgcgca tctgggcgcc gaagttcgcg atgtggctga gcatcccgcc gggatcgcgt
      481 cgtcgtatcg ctgccggatc ctcgagcatg ttgcccatct cagcggtggt catttcagcc
      541 ggcctcgccg cgcacgaggg cggagccctc cgccaggatt ccgtcacgga ggtccgccga
      601 agtcgcctcg gcgtagacac gaacgagtgg ctctgtgcca ctgaagcgca cgagcagcca
      661 cgacccgtcg tcgagccaga acttgaagcc gtccttcgtc gatagctcgt ccgtccgaat
      721 gaccggatgg cccgccaccg acgtgggcgc ctgtgcgacg agctcgacga gcagccgatc
      781 cttcagtgct gggtaggcct ggcggtcgac atggacgtcc gagcgcaggt agaacgactg
      841 gccggcaatc gaatgcaggt gttccacggc cgccgacacc ggccagcggc cagcctcgcg
      901 ctcgcggatg aacaggtcga gcagcagcag gtcggcgtaa atgccgtcgc gctctggcag
      961 gtgcatgccg aagccgtagc ccccggactc ctcgccgccc atcatcgcgc ccgtctcgat
     1021 catcttcggc ccgacgtact tgaagccgac cggcgtctcg aatacgtcga cgccgtactt
     1081 ctcgcccagc cggccgacca tcgaggtctc gttgatcgtc gacaccaccg gctggcgcag
     1141 tccgcggtgt tcgaccatgt aatacatcag caggcccatg acctggagct ggtggatgaa
     1201 ggtgcccttt tcatcggccg caccggcccg gtcggcgtcg ccgtccagga gcaaaccgag
     1261 gtcgtagccg cccgcggcga gcagcgatag tgactcgtcg atgttgggcc ggatgggctc
     1321 tgggttcacc ccaccgaaaa acgggttgcg ctcctggtgg atctcggtga tctggatccg
     1381 accacccgca agcagccgac ttatccagcc ggcgccggcg ccccacatcg gctcgaccag
     1441 cacgcgcatg tcggccgcct tgagccgatc gaggtccagc gatcgcctgg tgtactcgac
     1501 atacccggga tacgggtcgt agagctcaac cttgcctgag gcaactgcct ctgcgtatgc
     1561 ctgccgcggc ggctgcgacc cggcatagtc gcggatagtc ttctcgatga ccgccagcat
     1621 gtccgggctc gctgccgcgc cgctgggaga cttcaccttg aagccgttgt ccgggtacgg
     1681 gttgtgcgag gcggtgatca tgattccgca tgccgcaccg cgctcgacca cctcgtacga
     1741 ggtcatttgt gtcggtacgg cgttctgcga catggcgacc gggatgtcgt aggcgagcag
     1801 cacctcggcg gccgcctggg cgaagaactc cgacgcgaac cgccggtcgt aggcgatgac
     1861 tacgcccttc tggcttgtgc cggctgacct cgcccactcg gctacgcctt gggcgcagcg
     1921 gcggacgttt tcgtacgtgt agtcctcgcc gatgcgcgct cgccagccgt ccgtgccgaa
     1981 gacgatcggt ggaagaggcg cgctcgcgct gccgtcggtc acaggtgctc ggtctccttt
     2041 gcctgggtaa ggcgttcgac gatcgcgcgc acttcctgcg ccttatcggc ggcacagacg
     2101 aggacgacgt ccgtggtgtc gacgacgatg gtatcggcca gaccgatggt cacgaccagc
     2161 ctgccaccgg tcgagtgaac gaggacgttg ctggatgcaa ccgattcaga gcgtccgaca
     2221 gccaccacgc cgtgacgctc gcgcgtgcgc gccgccaacg cgcgatggag ggcgtcccag
     2281 ctgccgagat cgctccaccc aacatcagcc gggacgacct tgactttgcc ctcgaccgaa
     2341 gccggctcca gcagcgcgcg gtcgattgag atatcgggca gctcctcgta gatcgaggtg
     2401 agcgatttgc cggtgtcgag gccgcggcgg atgccgttgc cgatctgcgg cgcatatttg
     2461 gccaagccag cgagaagtgc gtcgtggcgc cagacgaaga tgcccgcgtt ccaccaagcg
     2521 ccgtttggac tggcgaggag ctccagggcg cgctcgcgtg gtggtttctc gacgaaccgt
     2581 tcgactcggc gcgcgcccac ctctgcttcg tccgcggacg ccccgacgat gtagccgtaa
     2641 cccgtttcag ggcccgtcgg ctcgatgccg agcgtgacca gcgagccgtc ctgggccgcc
     2701 tgtgcggcca ccccgagggt ccgtcggaaa ccctcctcgt cggcgatgaa gtgatcggcg
     2761 gggaggacga ccatcacgtc ttcgtcgcgg cggcccgaca ggatcgcagc gagtgccacg
     2821 gccgcggcag tgttgcgacc gaacggctcg cccagtcgct ggcgcgacgg gatctgcggc
     2881 gcctgctgct ccaccaacgg caggtgccgc tgctccgaga cgacgatcgt tgccgcgccg
     2941 tcgatgagcg gctcgatgcg ccgaagcgtc agctggaaga ggctctcagc gccgagcaac
     3001 gggaggaatg gcttcgggcg cgcccgccgg ctcagtggcc agaggcgggt gccgcctcca
     3061 ccggcgagga ctacggcgta catgcccggc cattctatgt gtcggcccgg ctggcccctg
     3121 ttcgtcgcta gaagctgggc ggcgttatcg agaacaggac gcgagccggc ttcgagccgc
     3181 cgttcctgtt gcgatgcggc aggcgtgagg aatgagtaac ggtgtctccc tcctggaggt
     3241 ggtaatgctc gtcggcgacc cacaggtcca gctcgccctc gagaacgagg accacctcct
     3301 catctgattc atgggtgtat ggctcgtcgc ctgtgccgcc cccgggctcg atagtcgaca
     3361 ggatcacctg gagtcgcgtc gcggtcggtg gcgtgaggaa ctcgtcgaca gcgccgagcc
     3421 cggcgtaggt gacgtgccgc cgctcgtcgc gccgcactac gcggccggcg gttgctgggc
     3481 cggcgaagag ctccgcgatg gagagcccga gcccgcgggc gatgcgctgc aacgaagcca
     3541 ccgatgggct ggccacgcca cgctccacct gcgaaaggaa cgactcggtg accccagcac
     3601 gaccagcgac gtcgcgcagg gtcatgccac gggcccgcct ttgaaggcgc agctgagccg
     3661 caaggtcgag atcggtgtct tcaaccggcg ccgattggtg ccgtgctcgc gtggtcatcc
     3721 aaattgaagt gtaacttcag atcacctgcg ttcggacccc agtcgaaagt tgacttggac
     3781 gcccactctt cctacgatcg cctaacgcgc taaagcggtg ctgcagtcgt cacctgagcc
     3841 ggagcagcgc gcgaggaggt cacatgcacg ccgcccccgc cgttctgcgc tcggccgcac
     3901 ttttcagtgc cacggcactg ctcgttgtcg catgcgctgg ttccgggtct tccccggcac
     3961 cgtcggccgc accttccaca gcgccctcga tcgggccgtc ggcaggtccg tccgcaagtc
     4021 cttccagcgc gccgtcggca gctgaatcga aggcgcctac ggcaggccca acagagggtg
     4081 ctgttaccgg cagcctgtcg gtgctcgaat ggtcgggcta cgaggacccg ctgtactggg
     4141 ccgacttcgc ggagaagtac cccgacgtcg aggttaactt cgacttcgga atctcggacg
     4201 ctgacatcta cggcaagatg aaagccggct cccagtcgga tctcttccac gcctacaccg
     4261 gatggctgca gttctatgtt gacgagggtc tagtccagga gatcgacacg accaagctcg
     4321 ccaactggga caaggtgccc gaccacttca agcaggtcgg ccagatcgac ggcaagcagt
     4381 acttcctgcc cttcgactgg ggattcacat caatcctcta caacaccgag cacgtcgacg
     4441 aggtgaccag ttggtcggca ctctttgacg agacctacga caaccacgtc tccatgtggg
     4501 atgacggtcc cggtgcagtg actgtgtctt cgtacatcca cgattacgac gagaccgcgg
     4561 tcac
//
LOCUS       DATLCO010000216         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4054959, whole genome shotgun sequence.
ACCESSION   DATLCO010000216 DATLCO010000000
VERSION     DATLCO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4054959"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VMZ33_05245"
     CDS             <1..1269
                     /locus_tag="VMZ33_05245"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HUR16668.1"
                     /translation="SGGLDTSVAVAWLREQFDVEVVTLTVDLGGGSLRGGVGERAMSS
                     GATRAYVVDARKQFVQEFVWPSLQAGALYQDAYPLATALARPLIARLLVEVAAREGAD
                     AVAHGCTGKGNDQVRFDVATKALDPRLRIIAPLREGMDMSRDEEIEYAQARGIEVPVT
                     KSSPYSVDVNMWGRSIEAGILEDPWTTPPADVYAWTAAPEDAPPEAAEITIGFEAGVP
                     VSLDGAALDAVAIVERLNELAGSHGVGRIDHVEDRLVGIKSREIYEAPAAVTLHAAHR
                     ALESLTLSKEQLRFGRLVRGEIAQAIYDGLWYSALQVDLRSYVATAQESVTGEVRVRL
                     QRGSTSVVGRRSLSSLYDRSLATYDTGDQFDHASAVGFISIWGLPLRTEAARAAARGA
                     KSALAEPLLDRLSVEVTDGSAEAARAGSSA"
     gene            1229..>2269
                     /gene="argH"
                     /locus_tag="VMZ33_05250"
     CDS             1229..>2269
                     /gene="argH"
                     /locus_tag="VMZ33_05250"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HUR16669.1"
                     /translation="MAAQRRPGRARPPETGVKTWGGRFSQSPDPRAAEFGRSIEIDAE
                     LAVDDLTASIAHARGLAAAGIVTPAEAEQLVAGLSSLLEEAHAGSLEWDPDLEDVHMN
                     LEALLEQRVGAVALKLHTGRSRNDQVSTDLRLWLRRTLDDIDMRVVELERALAGQALR
                     HADTVMPGHTHVQPAQPVLLAHHLLAHVEMLERDRGRLADARRRANVSPLGSGAVAGA
                     GFVLDRQAVATELGFDGVTANSIDASGDRDFVAEALFAFALAMIHLSRMAEEIAWWSN
                     PRFGFIRVADAFSTGSSMLPNKRNPDPVELVRGRASGVVGALTGVLALLKGLPLGYQR
                     DLQEDKAPLFAAA"
BASE COUNT          331 a          757 c          803 g          378 t
ORIGIN      
        1 tcgggcgggc ttgacacatc ggtcgccgtc gcgtggctgc gtgagcagtt cgacgtcgag
       61 gtggtgacgc tcaccgttga cctgggtggc ggatccctgc gtggaggcgt tggcgaacgg
      121 gcgatgagct ccggcgccac ccgcgcgtac gtcgttgacg cgcgaaagca gttcgtccag
      181 gagttcgtct ggccgagcct gcaggctggg gcgctctacc aggacgccta tccgttggcc
      241 acggcgctcg cccggccgtt gatcgcgcga ctcctcgttg aggtcgccgc gcgagagggt
      301 gccgatgcgg tcgcgcacgg ctgcacgggc aagggcaatg accaggtgcg ctttgatgtc
      361 gcgacgaagg ccctcgaccc acggctgcgg atcatcgcgc ccctccgtga gggcatggat
      421 atgagccgcg acgaggagat cgagtacgcc caggcgcgtg gcatcgaggt gccggtgacc
      481 aagtcttcgc cgtactcggt cgacgtcaac atgtggggcc gatcaatcga ggccggcatc
      541 cttgaggacc cctggacgac gccgccggcc gacgtgtacg catggaccgc cgcgcccgag
      601 gatgcgccgc ctgaggccgc ggagatcacg atcggcttcg aggccggcgt gcccgtgtcg
      661 ctggacggcg ccgcgctgga tgccgtggcg atcgttgagc ggctgaatga gctggctgga
      721 tcgcatggcg tggggcgcat cgaccacgtc gaggatcgcc tcgtaggtat caagagccgt
      781 gagatatacg aggctccggc cgcggtgaca ctccacgccg cgcaccgcgc tcttgagagc
      841 ctgacgctct ccaaggagca gctgcgcttc ggccgcctcg ttcgcgggga gatcgcccag
      901 gccatctacg acggcctctg gtacagcgcc ctccaggtcg acctgcgctc gtacgtggcg
      961 acggctcagg agagcgtgac cggcgaggtc cgtgtgcgcc tccaacgagg atcaacctct
     1021 gtcgtcggcc gacgcagcct cagttcgctt tacgaccgct cgctggccac gtacgacact
     1081 ggcgaccaat tcgaccacgc cagcgcagtg ggcttcatct ccatctgggg cctgccgctc
     1141 cgcaccgagg cagcgcgggc cgcggctcgc ggcgcgaagt cagccctggc cgagccgctg
     1201 cttgatcggt tgtcggtcga agtcaccgat ggcagcgcag aggcggcccg ggcgggctcg
     1261 tccgcctgag actggcgtga agacgtgggg cggacggttc tcgcagtcgc ctgacccgcg
     1321 ggcagctgag ttcgggcggt cgatcgagat cgacgcggag ctcgcggtcg acgacttgac
     1381 cgcgtcgatt gcccacgcac gaggacttgc cgcggccggg atcgtgacgc cggccgaggc
     1441 tgagcagctc gtcgccggcc tttcatcgtt gctcgaggag gcgcacgcgg gcagcctgga
     1501 gtgggatccg gacctcgagg acgttcacat gaatctggag gccctgttgg agcagcgcgt
     1561 cggtgcggtc gccctgaagc tccataccgg gcgatcccgc aacgaccagg tctcgaccga
     1621 cctgcgcctc tggttgcggc ggactttgga cgacatcgac atgcgcgtgg tcgagctcga
     1681 gcgggcgctc gctggccagg cgctgcgtca cgccgacacg gtcatgcccg gccacaccca
     1741 cgtacagccg gcgcagccgg tgctgctcgc gcaccacctc ctggcacacg tcgagatgct
     1801 cgagcgcgac cgcggccgac tcgccgatgc gcggcggcga gccaatgtgt cgccgctcgg
     1861 ctcgggggcg gttgcgggcg caggcttcgt gctcgatcgg caagccgtcg ccaccgagct
     1921 tgggtttgac ggcgtaacgg ccaactccat tgatgcctcg ggcgaccgtg acttcgtcgc
     1981 cgaagcgctg ttcgccttcg ccctggcgat gatccatttg tcgcggatgg ccgaagaaat
     2041 tgcctggtgg tcgaacccgc gctttggctt catccgggtc gccgacgcct tctcaactgg
     2101 ctcctcgatg ctccccaaca agcggaatcc ggacccggtc gagctcgttc gcggacgcgc
     2161 ttcgggcgtc gtcggtgcgc tgacgggtgt cctggcgctg ctcaaagggt tgccgctcgg
     2221 gtaccagcgc gatctccagg aggacaaggc gccactcttc gccgcggcg
//
LOCUS       DATLCO010000217         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9288910, whole genome shotgun sequence.
ACCESSION   DATLCO010000217 DATLCO010000000
VERSION     DATLCO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9288910"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..2829
                     /gene="aceE"
                     /locus_tag="VMZ33_05255"
     CDS             112..2829
                     /gene="aceE"
                     /locus_tag="VMZ33_05255"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HUR16670.1"
                     /translation="MFDQFGDFKRQLPDRDPQETTDWVQSLDSVVESEGRARAQFLLY
                     KLLKRARQHNLGLPPLTATRYINTISPEQEPEFPGDEAMELRIRRMVRWNAVAMVIRA
                     NSRAPGIGGHLATYASAATLYEVGFNHFFRGKDADGMGDQIYYQGHAAPGIYARAFLE
                     GRLSEDQLDHFRQEVQPDRGLSSYPHPRLMPNFWEFPTVSMGLGPLSAIYQARFNRYL
                     QHRGIKDTSNNHVWAFLGDGETDEPEAMGSLSIAAREGLDNLTFVVNCNLQRLDGPVR
                     GNGKIIQELESVFRGAGWNVIKVIWAREFDELLARDVDGVLVDKMNDTLDGEFQKYSV
                     ETGAYIREHFFGPDPRLQELVAHFSDDEVAKLRRGGHDYRKVYAAYDAATKAKGRPTA
                     ILAKTVKGWTLGTGVEGRNITHQAKKLTEQELKIFRDRLQIEIPDAKLKDAPYYHPGP
                     NAPEIQYLNERRASLGGPIPRRVVVTKDFELPTDSVYGEFESGSGTQEASTTMAFARL
                     LRNLLRDKVLGDRIVPIIPDEARTFGMDPLFKEVGIYSALGQLYEPVDSNLVLSYREA
                     KDGQVLEEGITEAGSSASFQAAGTAYATHAEPMIPFYIFYSMFGFQRTGDEFWAFADS
                     RGRGFLLGATAGRTTLNGEGLQHEDGHSLVVASTIPTIRVYDPAFAYETAAIVKDGIR
                     RMYGEAPEDVLYYLALYNENHVMPARPEWVSDEDIVRGLYRFKTAPEGVLGENGAPPL
                     RATLLAGGPIMQQALRAQAMLVERGVAAEIWSALSYQLLRNEALEADRWNRLHPAQAA
                     REPLVTQLLREPAEQGPIIAVSDFITAWPDMIARWVPGGWWRSLGTDGFGRSDTREAL
                     RRFFEIDAESIAAATLAELARCGRLEASEAERHIKDLGLDPEASFALRS"
     gene            2967..3110
                     /locus_tag="VMZ33_05260"
     CDS             2967..3110
                     /locus_tag="VMZ33_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16671.1"
                     /translation="MNEYSVGTLDLSGGGLPAAHLREHLVSGIRVRYTTRDNILIHYV
                     DAE"
BASE COUNT          561 a         1031 c         1016 g          527 t
ORIGIN      
        1 gtcccgccga tgtcgatgcc catgccacgt gtcgccacgg ccgaagcata cggcgctcat
       61 ggaaggaggc ccgcgggacg cttccgggaa gggccctaaa ctgcgtagga catgttcgat
      121 cagttcggcg acttcaaacg ccagctgccc gaccgcgatc cccaggagac aaccgactgg
      181 gttcagtcgc tcgactccgt ggtcgaaagc gaagggcgcg cgcgggcaca attcctgctc
      241 tacaagcttc tgaagcgtgc gcggcagcac aacctcggcc tgcctccgct cacggcaacg
      301 cgctacatca acacgatctc gcccgagcag gagcctgagt tcccggggga cgaggcaatg
      361 gagctgcgca tccggcgcat ggtccgctgg aacgccgtgg cgatggtcat ccgcgccaac
      421 agtcgggcac ccggaatcgg cggtcacctg gcgacgtatg catccgcggc gacgttgtac
      481 gaggtcggct tcaaccactt cttccgcggc aaggacgccg acggcatggg cgaccagatc
      541 tactaccagg gccatgctgc accgggcatc tacgcccggg cgttcctcga gggccgcctc
      601 tcggaggatc agctcgacca cttccgccag gaggttcagc ctgatagggg cctgagttcg
      661 tatccgcacc cgcggctgat gcccaacttt tgggagtttc ccaccgtttc gatgggtctc
      721 ggcccgctga gcgcgatcta tcaggcgagg ttcaaccgct acctccagca ccgcggcatc
      781 aaggacacat cgaacaacca cgtctgggcg tttctcggag atggcgagac ggatgagccg
      841 gaggcgatgg gctcgctgtc gatcgcggcg cgcgaaggcc tcgacaacct gaccttcgtc
      901 gtcaactgca acctgcagcg cctcgatggc cccgtacgtg gcaacggcaa gatcatccag
      961 gagctcgagt cagtcttccg cggcgccggt tggaatgtca tcaaggtcat ctgggcgcgc
     1021 gagttcgatg aactgctggc ccgggacgtg gacggcgtgc tcgtcgacaa gatgaacgac
     1081 accctcgatg gcgagttcca gaagtactcc gtcgaaactg gggcgtacat ccgggagcac
     1141 ttcttcgggc ctgatccgcg gctacaggag ctcgtcgcgc acttctcgga cgacgaagtg
     1201 gccaagctcc gtcgcggtgg ccacgactac cgcaaggtct acgccgcgta cgacgccgcg
     1261 accaaggcaa agggccggcc gacggcgatc ctcgccaaga cggtgaaggg ctggacactg
     1321 ggcaccggcg tcgaaggccg caacatcacc caccaggcga agaagctgac cgagcaggag
     1381 ctcaagatct tccgcgatcg gctgcagatc gagatccccg acgcgaagct caaggacgcg
     1441 ccctactacc accctgggcc gaacgcgccg gagatccagt acctcaacga gcgacgcgct
     1501 tccctgggcg ggcccatccc gcgtcgtgtg gtcgtgacga aggatttcga gctgcctacc
     1561 gatagcgtct acggtgagtt cgagtcgggt tccggcactc aggaggcgtc gaccacgatg
     1621 gccttcgccc ggctgctgcg caacctgctg cgcgacaagg tgctgggcga ccggatcgtg
     1681 ccgatcattc ccgacgaagc acgcaccttc gggatggacc cgctcttcaa ggaggtcggg
     1741 atctactcgg ccctgggaca gctgtacgag ccagtcgact cgaacctggt cctgtcgtat
     1801 cgcgaggcca aggacggcca ggtgctggag gaggggatca ccgaggccgg ctcatcggct
     1861 tcgttccagg ccgccggcac ggcgtacgcc acgcacgccg aaccaatgat cccgttctac
     1921 atcttctact cgatgttcgg cttccagcgc accggcgatg agttctgggc cttcgccgac
     1981 tcgcgtggcc gcggcttcct gctcggcgcg acagccggcc ggaccacgct caacggcgag
     2041 gggcttcagc acgaggatgg gcactcgctc gtggttgcgt ccacgattcc gacgatccgc
     2101 gtgtacgacc ccgcattcgc gtacgagaca gccgcgatcg tcaaggacgg cattcgccgc
     2161 atgtacggcg aggcacccga agacgtcctg tactacctgg cgctctacaa cgaaaatcat
     2221 gtgatgccgg cgcggcccga gtgggtcagc gacgaggaca tcgttcgtgg cctgtaccgg
     2281 ttcaagaccg cgcctgaggg cgtccttggc gagaacggcg cgccaccgtt gcgtgccaca
     2341 ctcctggccg gcggtccgat catgcagcag gccctccgcg cgcaggcgat gctcgttgag
     2401 cgtggcgtcg cagcggagat ctggagcgcg ctctcgtatc agctgctgcg caacgaggcg
     2461 cttgaggcgg atcgctggaa ccgcctgcat ccggcacagg ccgcccgtga gccgctcgtc
     2521 acccaactct tgcgtgagcc agccgagcag gggccgatta tcgccgtgag cgacttcatc
     2581 acggcctggc cggacatgat cgcccgttgg gtccccggtg gttggtggcg ctcgctgggc
     2641 acggatgggt ttggccggag tgacacccgt gaagcgctgc gccgcttctt cgagattgac
     2701 gccgagtcca tcgcagccgc caccctcgcc gagctggcgc gctgcggtcg cctcgaggcg
     2761 agcgaggctg agcgacatat aaaggatctg ggactggacc cggaggcatc attcgcgctg
     2821 cgctcctgat cctgtgtctg ttcgttggtg gtgcgtgcga gtcgccgacg aacgtcacga
     2881 agacggcagt cggcatcgtc gtcgctgctg aagggccaaa tgcagcggag gtcgttagcg
     2941 tgactgtccg agacgatggt ggtcaggtga acgaatactc ggtcggcacc ctggacctgt
     3001 ccggcggcgg tcttccggcg gcgcacctgc gtgagcatct cgtgtcgggc atccgtgtcc
     3061 ggtacaccac tcgcgacaac atcctgatcc actacgtcga cgccgaatga cgaccgacca
     3121 gccgaccgat cttcg
//
LOCUS       DATLCO010000218         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1842678, whole genome shotgun sequence.
ACCESSION   DATLCO010000218 DATLCO010000000
VERSION     DATLCO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842678"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VMZ33_05265"
     CDS             <1..536
                     /locus_tag="VMZ33_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUR16672.1"
                     /translation="LAHELGSTTDLPDAPLAVTAPGDESGTYDSFIELALGDLIEERA
                     AEARLRTPGDIYVASANDNAIIQGVGGFPTSLGFVGLYYAEQNAESVKTLAVDGGDGC
                     VAPTAETVSDGTYPISRPLFIYPNLSHAAENAAISAYVDFYLSDAGIANVTQVGYVAL
                     PADELSATRDAWAAAKP"
     gene            628..1566
                     /gene="pstC"
                     /locus_tag="VMZ33_05270"
     CDS             628..1566
                     /gene="pstC"
                     /locus_tag="VMZ33_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HUR16673.1"
                     /translation="MTAAEPAASLTITDLRGSPARRRRELIIRSAFFLAAATAVLISA
                     LIVFTLLGKALQFISQVEFASLFSGSWRPRANDFDLLSLIAGTMVIAGIAMAVAAPLG
                     LGSAVFLAEYASPRVRRMVKPVLELLAAIPSVVLGFFALTVLSPAVIDPFCPGPTPIF
                     NMAAAGVAVGILVTPLVASIAEDAMYAVPASLREASYGLGARKRTTSLRIVFPAAVSG
                     IVAALIVGFSRAIGETMVVALVAGGVGGAVFNLNPCLPGQTMTAAMTAQAVGSDQAAG
                     GLAGASLFFLGLLLFLMTLVLNVLSERFVRRIRSAY"
     gene            1566..>2036
                     /locus_tag="VMZ33_05275"
     CDS             1566..>2036
                     /locus_tag="VMZ33_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16674.1"
                     /translation="MADIARLRTPATTLAVERALIGTKRPIGEMIFHGVLFSALVVAL
                     AVLVWLLSDVFTRSMPVWTERGLASFATGNLSSDPAKAGVAQGLFGSVMLMLIVVVTA
                     IPIGIAAAIYLEEYAADNRLTRILNTTVRNLAGVPAVVYGLLGLGVFVALLDRDD"
BASE COUNT          294 a          721 c          630 g          391 t
ORIGIN      
        1 cactcgcaca cgagctcggc tcgaccacgg acctgcctga cgcgccactc gcggtcacgg
       61 ccccgggcga cgagtcgggc acgtacgaca gcttcatcga gctggcgctc ggcgacctga
      121 tcgaggagcg cgccgctgag gcgcggctcc ggacaccggg cgacatctac gtcgcgtcgg
      181 ccaacgacaa cgcgatcatc cagggcgtcg gcgggttccc aacgtctctc ggcttcgtgg
      241 gcctctacta cgccgagcag aacgctgagt ccgtcaagac cctcgctgtt gacggtggtg
      301 acggttgtgt cgccccaacg gcagagacgg tcagcgacgg cacttacccg atctcacgcc
      361 cgctgttcat ctacccgaac ctcagccacg ctgccgagaa cgcggcgatc agcgcctacg
      421 tcgacttcta cctttcggat gcgggtatcg cgaacgtgac ccaggttggt tacgtcgccc
      481 tgccggccga cgagctcagc gccacccgcg acgcgtgggc cgcggccaag ccctagctcc
      541 tactacgcca cgaatccgaa catcctgctc ccagcgcgca ggccggcgac aacgccggcc
      601 tgcgtgtcta tctgatagaa gggccccgtg actgcagccg agccagcagc atcactgacg
      661 atcaccgatc tgcgtggcag tcccgcgcgc cggcggcgcg agctgatcat ccgctcggcc
      721 tttttcctgg cagccgcgac tgccgtgctg atctcagcgc tgatcgtctt tacgctgctg
      781 ggcaaggcgc tccagttcat cagccaggtc gagttcgcga gcctcttctc cggttcgtgg
      841 cggccgcgcg caaacgactt tgatctgctg tcactcatcg ccggcaccat ggttatcgcg
      901 ggcatcgcca tggccgtggc tgcgccgctg ggtctcggct cggcggtctt cctggctgaa
      961 tatgcatcgc cccgggttcg tcggatggtc aagccagtcc tcgaactcct cgccgcaatc
     1021 ccgagcgtcg tcctgggctt cttcgcgctc acggtccttt ctccggcagt gatcgacccc
     1081 ttctgccccg gaccgacacc gatcttcaac atggccgccg ccggtgtcgc cgtgggcatc
     1141 ctggtcacgc cgctcgtggc gtcgatcgcc gaggacgcca tgtacgccgt acccgcctcg
     1201 ctgcgcgaag cctcctacgg gctcggcgct cgcaaacgga cgaccagcct gcgcatcgtc
     1261 ttcccggcag cggtttcggg catcgttgcc gcgttgatcg tcgggttctc acgcgccatc
     1321 ggcgagacga tggtcgtcgc gctcgtcgcc ggcggcgtcg gcggagcggt ctttaatctc
     1381 aacccgtgcc tgcccggtca gacgatgacc gcagcaatga ccgcccaggc ggtcggttcc
     1441 gatcaggcgg ccggcggttt ggcaggggcc agcctcttct tcctgggcct cctgctgttc
     1501 ctgatgacgc tcgtcctcaa tgtcctgagc gagcgcttcg tccgccgcat tcggagcgcc
     1561 tactaatggc cgacatcgcc cgccttcgga ccccggccac gacgttggcc gtggagcggg
     1621 cgctgatcgg cacgaagcga cccatcggcg aaatgatctt tcacggcgtt ttgttcagcg
     1681 cactcgtggt ggcgcttgcc gtccttgtgt ggcttctatc ggacgtcttc acccggtcga
     1741 tgcccgtctg gaccgaacgt ggcctcgcca gcttcgctac cggcaacctg tcgtcggatc
     1801 cggcgaaggc cggcgtcgca cagggtctgt tcgggtcggt gatgctcatg ctcatcgtgg
     1861 tcgtgacggc gattccaatc ggcatcgccg ccgcaatcta cctcgaggag tacgcagcgg
     1921 acaaccgcct gacgcgcatc ctgaacacga cggtacgcaa cctcgctggt gtgccggcgg
     1981 tcgtctatgg gctgctcggt ttgggcgtgt tcgtggcgct cctcgaccgt gacgat
//
LOCUS       DATLCO010000219         5129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6137609, whole genome shotgun sequence.
ACCESSION   DATLCO010000219 DATLCO010000000
VERSION     DATLCO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6137609"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /gene="tsaB"
                     /locus_tag="VMZ33_05280"
     CDS             <1..510
                     /gene="tsaB"
                     /locus_tag="VMZ33_05280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HUR16675.1"
                     /translation="PGSFTGLRIGLATAKVIAYTLNVPVVGVSTTEALGIAAVGRDGL
                     SDAVAVTLPAGAADRYVHLLRVDGESVHSEVPPRLVVPGPSFDEAIGDAFLVAIDMAG
                     ASDLADGAIERGESALEGLARAMAQMGLARFNEGKHDDVALLTPAYVALPRGIAQAAV
                     DMAWSPDLR"
     gene            489..1016
                     /gene="rimI"
                     /locus_tag="VMZ33_05285"
     CDS             489..1016
                     /gene="rimI"
                     /locus_tag="VMZ33_05285"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HUR16676.1"
                     /translation="MVARPPLRLRVEPMTLADVDDVHRIERASFPVPWPDYAFRQEIE
                     TNRLAHYLVVRASKEAIAYGGLWMMVDEAHITTFAVLPLYRRQGVGARLMVDLMRLAR
                     DLDARVVTLEVRLSNQPARALYHRFGFRPVGVRPRYYSDNGEDALIMTTEELSTRSMR
                     DRMAALEKQYQEAGG"
     gene            1013..2095
                     /gene="tsaD"
                     /locus_tag="VMZ33_05290"
     CDS             1013..2095
                     /gene="tsaD"
                     /locus_tag="VMZ33_05290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HUR16677.1"
                     /translation="MSADGRILAIETSCDETAVAVVENGRQIVASVIASQVALHGVTG
                     GIVPEVAARAHLRWMIPVLEQAKDRAGITDFATEVEAVAVTEGPGLAGSLLVGITMAK
                     TLAWMHGLPLVPVNHLEGHIYAAWLIDPNEEPKPEPEFPVVALVVSGGHTFLVEMTDH
                     LQYRLLGQTVDDAAGEAFDKVGRLLGLPYPGGPAIMRAAEGAQSRDRVFPRAWLGETY
                     DFSFSGLKTAARREVARELGVDAREIDGNGTNLPERSVSELAYGFQESVVDVLTTKTL
                     RAAEEIGAQTVIIGGGVAANSVLRTRVEEGAAARGIRTVVPRPGLCTDNAAMIGAAGF
                     YRARAGARAGVDLSAKPSLKLALPTR"
     gene            2227..2955
                     /gene="rsmA"
                     /locus_tag="VMZ33_05295"
     CDS             2227..2955
                     /gene="rsmA"
                     /locus_tag="VMZ33_05295"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HUR16678.1"
                     /translation="MELAEIDPATPILEIGPGLGILTGALLQAGGRVTAVEVDQRLVH
                     HLRMRFESIPDDQLRLVEANVLDVPIAELVAAPYDVVANLPYHITSPTLHHLLGEEPR
                     PERLVLMVQREVAERIAAAPGDMSYLSVFVQYHADVRVARIVPASAFEPAPNVESAVL
                     VGRSKPRLLDAEAEERLWRLVQAGFRERRKMLHNVLPRQLPQIGRERITAALADVGIA
                     PDRRPQTVSVAEWIAFSASIGTFR"
     gene            2916..3800
                     /locus_tag="VMZ33_05300"
     CDS             2916..3800
                     /locus_tag="VMZ33_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16679.1"
                     /translation="MDRVQREHRHVPMNFTARPPAKVNLDLTVVGRRDDGFHELLSTF
                     LRVGLTDVLKMRATEGEADRLTVTGLPGVPIRGNLVLAAVEAVRARAGVSLPALEIEL
                     DKRIPAAAGLGGGSSDCASAIELAQACWGLALSPADVVDLAASLGSDVPFFATGHGAA
                     TVAGRGERVARADVSVAALILLTPPIELSTAAVFARYDDLGAAGGPDVDGANDLWPAA
                     TSLEPRLPELRSALAAATGVEWRMSGSGPTLFALYPSIEAAVADAQSLVNEHPATVGD
                     CLIHAVEPDGEDPAWRFP"
     gene            3797..4174
                     /locus_tag="VMZ33_05305"
     CDS             3797..4174
                     /locus_tag="VMZ33_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HUR16680.1"
                     /translation="MSLRAVATDDAPSAIGPYSQGIDAGNLVFVAGQVGRDPVSGDLA
                     DDIEGQTERAMKNIQAILEAAGLTWANVAKTTCLLANIEDFQAFNAVYVNFVSEPRPA
                     RATYAVKALPGGALIEIEAFAVR"
     gene            complement(4171..4710)
                     /locus_tag="VMZ33_05310"
     CDS             complement(4171..4710)
                     /locus_tag="VMZ33_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16681.1"
                     /translation="MGHTGPVGYHALRDTFYSNAGATCRYVGVYPSPGGYSYEGELKR
                     IFVRPPKVKGIAGYGTQKVGWLFTVERTTNTSGSWTTTYTSPWQYDMTTPSTLADFSQ
                     RSINVNVPADSNDEPPYHVYRVLVKMRWYRADGAVRGTATHRVDYYKEIYTNWPLGDG
                     TEESSTSDYCTGWVGIEIN"
BASE COUNT          812 a         1725 c         1727 g          865 t
ORIGIN      
        1 ccgggaagct ttaccgggct gcgcataggc ctcgcgacgg ccaaggtcat cgcctacaca
       61 ctgaacgtgc cagtcgtcgg cgtgtcgacg acggaggcac tcggcattgc cgcggttgga
      121 cgcgacgggc tgagcgatgc cgtcgccgtg accctgccgg ccggcgccgc ggaccgctac
      181 gtgcacctgc tcagagtcga cggtgaatcg gtccactccg aggtaccgcc gcgtctcgtc
      241 gtcccgggtc caagcttcga cgaggccatc ggcgacgcgt tcctcgtggc gatcgacatg
      301 gccggggcga gcgacctggc ggacggcgca atcgaacgcg gcgagtccgc gcttgagggt
      361 ctggcccgcg cgatggcaca gatggggctc gccaggttca acgagggcaa gcacgacgac
      421 gtggcgctgc tcacgcccgc gtacgtggcc ttgccgcgcg gcatcgccca ggcagcagtg
      481 gacatggcat ggtcgcccga cctccgctga ggctgcgcgt cgagccgatg acgctcgccg
      541 acgtggatga cgtccatcgc atcgagcgcg cctcattccc cgtgccctgg cccgactacg
      601 cgttccgtca ggagattgag accaaccgcc tggcgcatta cctggttgtg cgtgccagta
      661 aggaggccat cgcctacggc ggcctgtgga tgatggtcga cgaggcgcac atcacaacgt
      721 tcgccgtcct gccgctctac cgccgccagg gcgtgggcgc gcgactgatg gtcgacctga
      781 tgcggctggc tcgcgacctc gacgcccgcg tggtgacgct cgaggtgcgg ctctccaacc
      841 agccggcacg cgccctgtac caccgcttcg gcttccggcc ggtgggtgtg cggccacgtt
      901 attactcgga caacggcgaa gacgcgctca tcatgaccac cgaagagctc tccacgcgtt
      961 ccatgcgcga ccgaatggcg gccctggaaa agcagtacca ggaggccggc gggtgagcgc
     1021 ggacggtcgg atcctcgcca tcgagacatc gtgcgacgaa accgcagtcg cggtcgtgga
     1081 gaatggccgg cagatcgtgg ccagcgtcat cgccagtcag gtggcgttgc acggcgtgac
     1141 cggcggcatc gtgcccgagg tcgcggcacg ggcgcaccta cgctggatga ttcctgtcct
     1201 cgagcaggcg aaggaccgcg ccgggatcac cgatttcgcg acggaagtcg aggccgtggc
     1261 ggtgacggaa gggccgggtc tcgccggctc actgctcgtc ggcatcacca tggccaagac
     1321 gctggcgtgg atgcacggcc tgccgctggt gcccgtaaac cacctggagg ggcacatcta
     1381 cgccgcgtgg ctcattgatc cgaacgagga gcccaagcct gagcccgagt tcccggtcgt
     1441 ggctctcgtc gtgagcggtg ggcacacatt ccttgtggaa atgactgatc acctgcaata
     1501 ccgattgctc ggccagacgg tcgatgacgc ggcgggtgag gcctttgaca aggtcggtcg
     1561 actcctcggc ctgccctatc ccggcgggcc ggcaatcatg cgcgcggccg aaggcgcgca
     1621 gtcgcgcgac cgcgtgttcc ctcgcgcgtg gctgggcgag acctacgact tctcgttcag
     1681 tggcctcaag accgctgcgc gccgtgaggt cgcgcgcgag cttggagtgg acgcccggga
     1741 gatcgatggc aacgggacga acctgccgga acgatccgtc tcggagctcg cgtacgggtt
     1801 tcaggagtcg gttgtcgacg ttctaacgac caagacgctc cgcgcagccg aggagattgg
     1861 cgcgcagacg gtgatcattg gtgggggagt ggccgcgaac agcgtgctgc ggacgcgagt
     1921 cgaggagggc gcggctgcgc gcggaatccg aaccgtcgtg ccgcgccctg gtctctgcac
     1981 cgacaacgcc gcgatgatcg gcgccgcggg cttctatcga gcgcgcgcag gtgcccgcgc
     2041 cggcgtcgat ctgtcggcca agccatcgct caagctggcg ctaccgacac gctgacctca
     2101 gacgagctcg acccaaagcg actttcggct gaacgtgtcg agcgctacct gcgcaggcat
     2161 gggctgaccg cgaacaagaa gcgcagccag aaccacctga tcgactccga ggtcctggag
     2221 gcgatcctgg agctggccga gatcgatccc gccacgccaa tactggagat cggaccgggg
     2281 ctggggattc tgaccggtgc gttgctccag gcaggtggtc gagtgacggc cgtcgaggtt
     2341 gaccagcgtc tcgtgcatca ccttcggatg cgcttcgagt ccattcccga cgaccagctg
     2401 cgactcgtgg aagcgaacgt cctcgacgtg cccatcgcgg agttggtcgc tgccccatac
     2461 gacgtggtgg cgaatctgcc gtaccacatc acgtcgccga cgctgcacca cctcctgggc
     2521 gaggaaccgc ggccggagag gctcgtcttg atggtccagc gcgaggtcgc ggagcgcatc
     2581 gcagccgcgc ccggggacat gagctatctg tcggtattcg tgcagtacca cgccgacgtc
     2641 cgcgtggcgc ggatcgtgcc cgcgtccgcc ttcgagccgg cacccaacgt ggagtcggca
     2701 gttctcgtcg ggcgcagcaa gccccggttg ctggacgccg aagccgaaga acgactgtgg
     2761 cggctcgtcc aagcgggctt ccgcgagcgg cgcaagatgc tccacaacgt gctgccgcgc
     2821 cagctcccgc agatcggtcg cgagcggatc accgccgcgc tcgccgacgt agggatcgcg
     2881 cccgatcggc ggccgcagac ggtgtcggtc gcggagtgga tcgcgttcag cgcgagcatc
     2941 ggcacgttcc gatgaacttc accgcgcgtc cgccggccaa ggtgaacctc gatctgacgg
     3001 tcgtcggccg ccgcgatgac gggtttcatg agctgctctc gacgttcctg cgcgtggggc
     3061 tgaccgacgt cctgaagatg cgcgccacgg aaggcgaagc ggaccgtctc accgttaccg
     3121 gcctgccggg agtgccgatc cgcggcaacc tcgtcctcgc cgccgttgag gccgtgcgcg
     3181 ctcgcgccgg cgtgagtttg ccggcgctcg aaatcgagct cgacaagcgc atcccggcgg
     3241 ccgcagggct cggcggtggc agcagcgact gcgccagcgc gattgagctc gcccaggcct
     3301 gctggggcct tgcgctctca ccagcggacg tggtcgatct ggccgcgtct cttggttcgg
     3361 acgttccttt cttcgcgacc ggccatggcg ccgcgaccgt tgcggggcga ggggaacgcg
     3421 tggcgcgggc ggatgtgtcc gtcgcggcgc tcattctgct gacgccgccg atcgagcttt
     3481 cgaccgcggc cgtgttcgcc cgctacgacg acctcggcgc ggccggcggc ccggatgtcg
     3541 acggcgccaa cgacctatgg ccagccgcga catcgctcga gccacgcctg ccggagctcc
     3601 gttctgcgct cgccgccgcc acgggcgtcg agtggcgcat gtccggatcg gggccgaccc
     3661 tgtttgcgct ctatccttcg atcgaggccg ccgtcgccga cgctcagtcg ctcgtcaacg
     3721 agcatccggc aacggtcgga gattgtctta tccacgccgt cgaacccgac ggagaagatc
     3781 cagcctggag gtttccatga gcctgcgtgc cgtcgccact gatgacgccc cgagcgcgat
     3841 cggtccctac agccagggca tcgacgccgg caatctcgtc ttcgtcgcgg gtcaggttgg
     3901 ccgcgacccg gtgtccggcg accttgccga cgacatcgag ggtcagaccg agcgcgcgat
     3961 gaagaacatt caggcgatcc tcgaggctgc cggcctgacc tgggcgaacg tggccaagac
     4021 gacgtgtttg ctcgcgaaca tcgaggactt tcaggcattc aacgccgtct acgtgaactt
     4081 tgtgagcgag ccgcgcccag cgcgtgccac ctacgcagtc aaggcgctcc ccggcggcgc
     4141 actcatcgag atcgaggcgt tcgccgtccg ttagttgatc tcgataccaa cccagccggt
     4201 gcaatagtcg ctggtcgagc tctcttctgt gccgtcgccg agcggccagt tggtgtagat
     4261 ctccttgtag tagtcgacgc ggtgtgtggc ggttccacgt acggcgccgt cagcgcggta
     4321 ccagcgcatc ttcaccagta ctcggtagac gtggtacggc ggctcgtcgt tgctgtccgc
     4381 agggacattc acgttgatgc tccgctggct gaagtcagcc agcgtcgacg gggtcgtcat
     4441 gtcgtactgc cacggactcg tgtacgtcgt ggtccagctg ccagaggtgt tggttgtccg
     4501 ctcgaccgtg aacagccagc cgaccttctg ggtgccgtac ccggctatac ccttgacttt
     4561 cggcgggcgg acaaaaattc gcttgagctc gccttcgtac gagtagccgc ccggcgacgg
     4621 gtagacgccg acgtaccggc acgtggcgcc ggcattcgag tagaaggtgt cacgcagcgc
     4681 gtggtagccc accgggccgg tgtgtcccaa gtcggtatca gccaacacgg gtgctgccga
     4741 tagtgcaagc agcgccaatc cagccagcgc tgatgccgcg acgccatgcc ggcgacgagt
     4801 ggaccctgac atgcctcgac cctcccaatc ccttccgagc gcgtcgcata cggcctccca
     4861 gcgcgggctc gtcgaactga gtatctgcgg tccgtcaacg atcggtcgct ggccccgttt
     4921 gaccgaatca gttccgcgac cgtagagtca cggcccctga tgtcctctgc ccaacctcca
     4981 tccgccaccg gcggtcgccg gccgacgtga ccgtcgtgag ctcgacaacc aaggcggtca
     5041 ttctggccgc tggcctcggc acgcgaatga aatcgcgaat cccgaaggtt cttcacccga
     5101 tctgcggccg gcccatgctt gcctatgtc
//
LOCUS       DATLCO010000220         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4064368, whole genome shotgun sequence.
ACCESSION   DATLCO010000220 DATLCO010000000
VERSION     DATLCO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4064368"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VMZ33_05315"
     CDS             <1..799
                     /locus_tag="VMZ33_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16682.1"
                     /translation="WLEDGLALVRRASDREAEAWMAWQLSDYWEFRGDFVLAIEYCRL
                     SVELAEQHGDVATGAARRVGLAWLEAHLGRWPATNGDGADDATVAPGEDQYLAWGTAW
                     RAVLAWRDRPAAGLELIRGGLGGMSTEARAPALWGARMAYRLGDEDVIRMGRSNTGGG
                     RTVAALVDAWMAAFLDDVASAPHGLARVVAEADRLGYRNFAQMARDDLALAVARSGRD
                     ASVEVRNAVEFARGLGIQPLLGPLPETRWLTAGPDDGGKVAVDAAAD"
     gene            783..1589
                     /locus_tag="VMZ33_05320"
     CDS             783..1589
                     /locus_tag="VMZ33_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HUR16683.1"
                     /translation="MPRLIDSHAHLQATAFDSDAEQVLAVARATGVERMLVPGWDLES
                     SRASVELARSAGVGASAGIHPHVASEQGASAWQAVAAMVSLPDVVAVGETGLDYDRGF
                     SPRDAQLANLRWHLGLARDSGKPAILHCRSKPGERDAQDDLIRELAEAGGPPALLHSF
                     SGPVDYAERALEMGLYVSFSGLAFRAGEEVTADVACLVPADRLLVETDSPYLSPPGAP
                     RRRNTPEWVRVTVAWLAQQRGTTLDELGPQLVSNYDRLFTKSESAPDPRR"
     gene            complement(1566..2327)
                     /locus_tag="VMZ33_05325"
     CDS             complement(1566..2327)
                     /locus_tag="VMZ33_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HUR16684.1"
                     /translation="MTVAARLERTYRVRFDEAGPDGFLRASGFVRYAQDMAWIHSESA
                     GFGRAWYRDRGLFWLVRGVELDLLEDVDYGTELVVSTEVIGFRRVIARRRSEFHLPES
                     ERPVATVLTDWVLLKDNGRPVQPPAEILEMFPPASNEFAPLRARVGEPPAHADEAAFS
                     VRRSEADPMGHVNNAVYVDYLDEGFLRRAARDGGLPVPRRYRVEFAGSALPGSRVTAR
                     GWDEQLAWSCTLTDENGHETLRATLETDSAAWVGG"
BASE COUNT          384 a          804 c          919 g          416 t
ORIGIN      
        1 gtggctggag gatggcttgg cgctcgtccg gcgtgcctcc gaccgcgagg cggaggcgtg
       61 gatggcctgg cagctctcgg attactggga gtttcgcggt gactttgtgc tggcgatcga
      121 gtattgccgc ctgtccgtcg agctggctga acaacacggg gacgtggcga ccggcgcggc
      181 cagacgcgtt gggctggcgt ggctcgaggc acatctcggt cggtggccag ccacgaatgg
      241 ggatggagcg gatgacgcaa cggttgctcc gggagaggat cagtacttgg cttggggaac
      301 agcgtggcgt gctgtgctgg cgtggcggga ccgtcccgcg gcggggctcg aactgatcag
      361 gggaggactg ggcgggatgt ccaccgaggc acgagcgccc gccctatggg gcgcccgaat
      421 ggcctatcgg ctgggcgatg aggacgtgat ccggatgggt cgatcgaaca cgggtggcgg
      481 tcgcaccgtg gccgcactgg tggacgcgtg gatggcggct ttcctcgatg acgtggccag
      541 tgcgccgcat ggactcgccc gcgtggtcgc tgaggccgac cgtctcggat atcgcaactt
      601 tgctcaaatg gcgcgcgacg atcttgcgct ggcggtcgcg cgaagcggac gtgacgcatc
      661 cgtggaggtt cgcaacgcgg tcgagttcgc acgtgggctc ggcattcagc cgctgctcgg
      721 accgctccca gaaacgcgat ggctgaccgc gggccccgac gacggaggaa aggtcgctgt
      781 cgatgccgcg gctgattgat tcgcacgcgc acctccaggc cacggccttt gattccgacg
      841 ccgagcaggt gttggccgtt gcacgggcca cgggcgtcga acgcatgctc gtgccagggt
      901 gggacctgga gtcgtcgcgc gcgtcggtgg aactggcgcg gagcgccggt gttggcgctt
      961 cggcgggcat ccacccgcac gtggccagcg agcaaggcgc gtcggcttgg caggcggtcg
     1021 cagcgatggt gtccctgccc gatgttgtgg cggtcggcga gacagggctc gattacgacc
     1081 gtggcttctc gccgcgcgat gcacagctcg ccaacctccg ctggcacctg gggctcgcgc
     1141 gcgacagcgg caagccggcg attctccact gccgttccaa gcccggggaa agggacgccc
     1201 aggacgacct gatccgcgag ctggcggagg ccggcgggcc accagcgctg cttcactcgt
     1261 tctcaggtcc ggtcgactac gccgagcgtg cgctcgagat ggggctgtac gtcagtttca
     1321 gcggtctggc gttccgggct ggggaggagg tgaccgctga cgtggcttgc ctggtgccgg
     1381 cggaccgact gctggtcgag accgattcgc cctacctgtc gccgcccgga gcgccgcgcc
     1441 gccgtaacac gcccgagtgg gtccgtgtca cggtcgcctg gctggcgcag cagcgcggca
     1501 cgactcttga cgagctcgga ccccagctag tctcgaacta cgaccgcctc ttcacgaagt
     1561 ccgagtcagc ccccgaccca cgccgctgaa tccgtctcca gggtcgcgcg gagggtttcg
     1621 tggccgttct cgtcggtgag cgtgcatgac cacgccagtt gctcgtccca accgcgcgcc
     1681 gtgacccgcg agccgggcag cgccgaaccg gcgaattcga cgcggtagcg gcgcggtacc
     1741 ggcagcccgc cgtcgcgcgc tgcccggcgc aggaagccct cgtcgaggta gtcgacgtac
     1801 acggcgttgt tcacgtggcc catggggtca gcctcgctcc gacggacaga aaatgcggcc
     1861 tcgtccgcat gagccggcgg ttcgccgacg cgcgcccgga gtggcgcgaa ctcgttcgaa
     1921 gcgggcggaa acatctcgag gatttcggcc gggggctgca cgggacggcc gttgtccttg
     1981 agcagcaccc agtcggtcag gacggtcgcc accggacgtt cggattcggg caggtggaac
     2041 tcactcctgc gccgcgcgat gacacgccga aagccgatga cctcggtcga gacgacgagc
     2101 tccgtgccgt agtccacgtc ttccagaagg tcgagctcca caccacgaac tagccagaac
     2161 aggcctcggt cgcggtacca ggcgcgtccg aagccggcag actcagagtg gatccaggcc
     2221 atgtcctggg cgtaacggac aaagccagag gcgcgtagaa aaccgtcagg gcccgcctcg
     2281 tcaaagcgga cccgataggt tcgttcgagt ctcgcggcga cggtcatcgc cgcgagtcta
     2341 tgactgtcgg ttgatcagcg agtatcgacg tccgatggtt ctgcttgcac cgcaggcgcg
     2401 gccagcatgt cgtcgaccgc ggcgtccttc cgattttcga aatcgggtcg cgcgtgcggc
     2461 atctggagcg gctgcgacac gttgacgtcg ggccgaactg gagcctgggc gccggcatcg
     2521 gtc
//
LOCUS       DATLCO010000221         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4822307, whole genome shotgun sequence.
ACCESSION   DATLCO010000221 DATLCO010000000
VERSION     DATLCO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4822307"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VMZ33_05330"
     CDS             complement(<1..707)
                     /locus_tag="VMZ33_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HUR16685.1"
                     /translation="MELLTDPQAWVALLALTALQIVLGIDNIVFISIMAAKLPPAERE
                     RARVLGLAGALITRIILLFGIFVIVSLTEPIFEVFGRHFSGRDLILLGGGLFLIAKAT
                     FEIHERLEGEESSGKAAAGTLTSVVIQIVILDIIFSLDSTITAVGMSDVLAIQVASII
                     LAVIVMLVASKPIGNVIERHPSLKILALSFLLLIGTSLIAEGFEISIPREYIYFAMGF
                     SVLVEMLNIRAHTRGKPL"
     gene            complement(1014..1874)
                     /locus_tag="VMZ33_05335"
     CDS             complement(1014..1874)
                     /locus_tag="VMZ33_05335"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HUR16686.1"
                     /translation="MTLAITDRRAQTLVDVDWAREHLDDPQVRFLEVDVDTASYDNGH
                     LPGAVGLNWTTQLNDTIRRDLASRDELQSLLRAAGINDHTLIVIYGDNDNWFAAWAYW
                     QLKYHGIDNVRLLNGGRKKVEAAGLPLTTDVASHAAGNITVPEGTNENLRAYRDAVLN
                     NASNGNRALVDVRSPEEFRGELAAPAHLPQERAQRKGHVPGAANIPWAKAVAEDGTFR
                     SDEELAELYGARGITPDKEIVVYCRIGERSSHTWFVLRELLGYPTVRNYDGSWTEYGS
                     LVGAPIEVGG"
     gene            complement(2291..2494)
                     /locus_tag="VMZ33_05340"
     CDS             complement(2291..2494)
                     /locus_tag="VMZ33_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16687.1"
                     /translation="MALPVPWDTAFGPRRGLVQWMAVGSRKDWPFAVLLVGVMPEPVL
                     VGLEALDDRMVVRDGVLGRVLRR"
     gene            complement(2500..>2892)
                     /locus_tag="VMZ33_05345"
     CDS             complement(2500..>2892)
                     /locus_tag="VMZ33_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16688.1"
                     /translation="DRWPLWATIGHLCCQRVSGLSGMVGEPGADTTPFPNALYNCPGD
                     EYLEPAMTAHELVAAIDSTFRIVEHCLDTWTMESLDEVMQRDFGDEIVEIKRGFYLQR
                     SLAHDVYHIAELNEALSRAGLPLIDLWD"
BASE COUNT          502 a          905 c          971 g          514 t
ORIGIN      
        1 agcggcttgc cacgcgtgtg ggcgcgaata ttgagcatct cgacgaggac cgagaagccc
       61 atcgcgaagt agatgtattc gcgcggaatg ctgatctcga agccctcggc gatcaacgaa
      121 gtcccgatga gcagcaggaa ggacagcgcc aggatcttca ggctcggatg gcgctcgatg
      181 acgttgccga tcggttttga cgccacgagc atcacgatga ccgccaggat gatcgatgcg
      241 acctggattg ccagcacgtc cgacatcccg acggccgtga tggtggagtc gagcgaaaag
      301 atgatgtcga ggatgacgat ctgtatgacc acgctcgtca gggtgcccgc ggcggccttg
      361 ccggagcttt cctcgccctc gagccgctcg tggatctcga acgtcgcctt ggcaatcagg
      421 aataggccgc caccgagaag aatcagatcg cgcccgctga agtgacgacc gaacacctca
      481 aagatgggct ctgtcagtga aacgatcacg aagatcccga agagcaggat gatgcgggtg
      541 atcagcgccc cggccaggcc caggacccgc gctcgctcgc gttcggcggg cggcagcttg
      601 gcggccatga tggagatgaa gacgatgttg tcgatgccca ggacgatctg aagggcagtg
      661 agcgcgagca gcgctaccca ggcttggggg tcggtcagga gctccattgg ctgacgagtc
      721 taccggcggc cggcatagcg gataaacaag aagaggaggg cacgtggccc tcctctggct
      781 ttcaaattga gcgggcgact acttggtcgc ggcgcccttc tgctttttgg tggacgtttt
      841 gggcttcttc gcgccgccct tgctgcccgg gcccttgtca cccatcgtcg agtccccttc
      901 agtgcgtgac tggtcggtat caatcctagc gggcgcagat gcaccccgga caacttccgg
      961 cacctgcatg gccatcgcct gccgccgtgg caaactcggc cggcgtcgga cgttcagccg
     1021 ccgacctcga tcggagcccc gacgagggag ccgtactcgg tccagctgcc gtcgtagttg
     1081 cgaacggtgg gatagccgag cagctcgcgc agcacgaacc aggtgtggct cgagcgctcg
     1141 ccgatgcggc aatagaccac gatctccttg tcgggcgtga tgcccctggc gccgtacagc
     1201 tcggccagct cctcgtcgga cctgaaggtg ccgtcttcgg ccacggcttt ggcccacggg
     1261 atattcgccg ccccggggac gtgacccttg cgctgggcgc gctcctgcgg caggtgcgcg
     1321 ggtgccgcga gctcgccgcg gaactcctcc ggtgagcgca cgtcgaccaa cgcgcggttc
     1381 ccgttcgagg cgttgttcag caccgcgtcg cggtatgcgc gcaggttctc attcgtgcct
     1441 tcgggaaccg tgatgttgcc cgctgcgtgt gacgcgacgt ccgtagtcag cggaaggccg
     1501 gcggcttcga ccttcttgcg gccaccgttg agcagccgga cgttgtcaat gccgtggtac
     1561 ttgagctgcc agtacgccca ggcggcgaac cagttgtcgt tgtcgccgta gatgacgatc
     1621 aacgtgtggt cgttgatgcc cgcggcgcgc agcaggctct gaagctcgtc gcgcgacgcg
     1681 agatcgcgcc ggatcgtgtc gttgagctgc gtggtccagt tgaggccgac ggcgccggga
     1741 aggtgcccgt tgtcgtacga agccgtgtcg acgtccacct caaggaaacg gacctgcggg
     1801 tcgtcgaggt gctcgcgcgc ccagtcgacg tcgaccaggg tctgagctcg gcggtcagtg
     1861 attgccaggg tcatcgggaa agtgctcctc ttgcgtgtga cgggacgtga ggtgggcgcc
     1921 acgcgcacac ggctgctgcg tgacgttcgg ggaagtcggg cggttggggt ggagttgcgc
     1981 agcgacgcct ccgtgcgagg cgttacgaga tcaggcggaa ggaaccccgt ccgcccaggg
     2041 acagaggcat agcctccggt agcgacagat ctcggcgcgc atggaggcgg catcgtaaac
     2101 gacgagcggc atatcggcat gcagggtctt gcacgccccg tcaccatgtc tgtacatggc
     2161 cgatgccccg atagatccga ctattccgcc gagtggcccc gggtgcgtgg agtgtctcgc
     2221 cacgcccgac ggctggtggc tccaccttcg acgttgcgcc gcgtgtggcc acgtcggttg
     2281 ctgcgattcg tcaccgtcgc agcacgcgtc caagcacgcc gtcacgcacg accatccgat
     2341 cgtccagagc ttcgagcccg acgaggactg gttctggcat taccccaacg agcagtacgg
     2401 cgaagggcca gtccttgcgg ctcccgacag ccatccactg gaccagccca cgcctgggcc
     2461 cgaaggccgt gtcccacggg actgggagcg ccaccttcac taatcccaga ggtcgataag
     2521 gggcaagcct gcccgcgaga gggcctcgtt gagctcagcg atgtggtaga cgtcgtgggc
     2581 gagcgatctt tggaggtaga agccgcgctt gatctccaca atctcgtcgc cgaagtcgcg
     2641 ctgcatcacc tcatcgaggc tttccatggt ccacgtatcg agacagtgct cgacgatgcg
     2701 gaaggtcgaa tcgatcgcgg cgaccaactc atgcgcggtc atggccggct ccaggtactc
     2761 gtcgccggga cagttgtaga gcgcgttggg gaatggcgtc gtatcggctc ccggctcacc
     2821 caccatcccg gagagtccag acacgcgctg gcagcacagg tggccgatgg tcgcccagag
     2881 cggccaccga tc
//
LOCUS       DATLCO010000222         3224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7627871, whole genome shotgun sequence.
ACCESSION   DATLCO010000222 DATLCO010000000
VERSION     DATLCO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7627871"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VMZ33_05350"
     CDS             complement(<1..297)
                     /locus_tag="VMZ33_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16689.1"
                     /translation="MAEPGVRVRGRRTRDRRIAAEIDRAHDTATGGSTRSRLPDLSAL
                     TNLLAQSGELRSLVDRYRTVREGRVGQNLRHVTYAQMPHGAKSFLAAALVVASGE"
     gene            complement(290..1021)
                     /gene="pth"
                     /locus_tag="VMZ33_05355"
     CDS             complement(290..1021)
                     /gene="pth"
                     /locus_tag="VMZ33_05355"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HUR16690.1"
                     /translation="MAVLRVLYGSDKSSMKIVVGLGNPGSQYEKTRHNVGWMVLDKIA
                     ERAGWIGHARARDAAATVYGRHNGLDLMLVKPTTFMNLSGVAVRKVLARQRAPLSDLL
                     VVVDDFDLPLGKLRVRERGSAGTHNGLRSIIGELGAQDFARLRVGIGEPKREGAARDH
                     VLSRFTAAERRVLDEAIDAAADAVEEWARQGPTYAANRWNSWQAEIDAAPGAATDPGP
                     DDEPKPDKHGIVRSSTGWRKLLGRG"
     gene            1225..2412
                     /locus_tag="VMZ33_05360"
     CDS             1225..2412
                     /locus_tag="VMZ33_05360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16691.1"
                     /translation="MVADGVPTLLLPYLLVGGDAGDATFLGLATLLAIATAALLQPLA
                     GKLSDRVGRRPVACIGVAAAGAGLAIIVISAGSTVVFVGLLVTLCGVSVAQAGQQALL
                     PDYLDTSRRGVASGLKGAFDVGGALLAFVVLAALLGSGEPTLAAVALFGVLGLSTVAG
                     ILLLARTRKVDRTTKTAAATSVSERAQFARIVMARFLFLLGIYAVGRFMFLFVADAWN
                     LSADDAAAQAGLALTVLAALTVAASIPAGWLADRLGRRPVMVAGAGLAAVGIALVPFT
                     RDPVGLIGAGVLLALGSAAFGSASWAALSEAVAPERSGWQLGLANLGTAGAAAAAGLF
                     GVVIDLGNGFRPEAGYVVAFGLAAVAALAGGALAWRWGSLSRARVPPAQFTTQTSATV
                     EAR"
     gene            2413..>3224
                     /locus_tag="VMZ33_05365"
     CDS             2413..>3224
                     /locus_tag="VMZ33_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16692.1"
                     /translation="MLQRIPIDVPTLLSQLYKPLQPIVQRIHYVRRGDRRYEPADILL
                     PRGYVAEVVCPGLTAPVHASFGPEGEMYVTESGHKSYSPPRVYRVDTGTGEKELVAEF
                     GGDRWIESGAATGAVWHDGSLYVANTDTIVKIDPTTGAISDVVTGLPGKGDHQANHPL
                     VGPEGKLYWGQGCVTNMGVVGADNFGYEWLPMFPETCDVPAEDIVLAGRNYEVPNVLG
                     NPLEKATTGAYVPFGTDTDFGQIIPGNPKASGAILRVDPDTKEVEAVAWGLR"
BASE COUNT          479 a         1079 c         1101 g          565 t
ORIGIN      
        1 ctcgccgctc gccacgacca gtgctgcggc caggaacgac ttcgcgccgt gcggcatctg
       61 cgcgtacgtg acgtggcgaa ggttctggcc gactcgacct tcgcggacgg tgcgatagcg
      121 gtccacgagc gaccgcagct cgcccgattg cgcgagcagg ttcgtcagcg ccgagaggtc
      181 gggtagccgc gagcgggtcg atccgcccgt tgccgtgtcg tgggcgcggt cgatctcggc
      241 ggcgatccgc ctgtcgcggg tgcgacgccc gcgtacgcgg acgcctggct cagccacgac
      301 cgaggagctt tcgccaaccg gtcgagctac ggacgattcc gtgcttgtcg ggcttgggct
      361 cgtcgtcggg cccggggtcc gtggcggcgc ccggtgcggc gtcgatctcc gcctgccagg
      421 agttccaacg gttggcggca tacgtcggtc cctgtcgagc ccactcctcg acagcgtcgg
      481 cggcggcatc gatggcttcg tcgagtaccc ggcgctcagc ggccgtaaag cgactgagca
      541 cgtgatcgcg cgccgctcct tcgcgcttcg gctcgccgat gcccacccgc agtcgagcaa
      601 agtcctgtgc gcccagctcg ccgatgatcg aacgcaagcc gttgtgcgtg ccggcgctgc
      661 cgcgctccct cacgcgcagc ttgccaaggg gtagatcgaa atcatcaacg acgacgagga
      721 ggtccgacag cggggcgcgc tggcgcgcga gcaccttccg gacagccacg cccgacaggt
      781 tcatgaaggt cgtcggcttg acgagcatca ggtcgaggcc gttgtggcgg ccgtagacgg
      841 ttgccgcggc gtcacgggcg cgggcatggc caatccagcc ggcgcgctcg gcgatcttgt
      901 cgagcaccat ccagcccacg ttgtgtcgcg tcttttcgta ctggctgccg gggtttccca
      961 accccaccac gatcttcatc gaactcttgt cggacccata caggacacgc aaaacggcca
     1021 cgcaaggagc ctcacgggtt cctcgggtgg cggctgggca tggtaccgcg aacgacgtaa
     1081 cgcgatacgt cgtttacgaa tcggatggcg tgagggctcg cgatccgtgg tccgggcgag
     1141 cactgtgccc ttgaccatta cgtagcggag ggcgcggagc tgcgcgtact gctgggggca
     1201 agcattggct ggctcggcgt cagcatggtc gccgacggcg tgccgacgct tctattgcct
     1261 tatttgctcg tcggcggcga tgccggcgac gccacgttcc tgggcctggc gacgctgctg
     1321 gcgattgcga cggcggcgct gctccagccg cttgccggca aactcagcga cagggtcgga
     1381 cgtcgcccgg tcgcgtgcat cggggtggcc gccgcaggtg ccgggctggc aatcatcgtg
     1441 atttcggctg gctcgaccgt cgtcttcgtc ggacttctcg tgacgctttg cggcgtaagc
     1501 gttgcccagg cgggacagca ggcgttgctg cccgattacc tcgatacgtc acgacgtggc
     1561 gttgccagcg ggctgaaagg cgcgttcgac gtgggcggcg cgttgttggc cttcgtcgtt
     1621 ctggccgcgc tcctggggag cggcgagcca acgctcgcgg ccgtcgcgct gttcggcgtt
     1681 ctcgggctca gcacagtggc gggcatcttg ctcctcgcgc gcacgaggaa ggtcgatcgg
     1741 acgacgaaga cagcggctgc cacctcggtg agcgaacgcg cacagttcgc gcgcatcgtc
     1801 atggcgcgat tcctgttcct gctcggcata tacgccgtcg gccgtttcat gttcctgttc
     1861 gtggccgacg catggaatct gtccgcagat gacgccgcgg ctcaagcagg actcgcgctc
     1921 acggtcctcg ccgcgctcac ggtcgcggcc tcgattcccg ctggttggct ggccgacagg
     1981 cttggtcgcc gcccggtgat ggtcgccggc gccggcctgg cagcggttgg catcgcgctc
     2041 gtgccgttca cacgtgaccc cgtcgggctg atcggtgctg gcgtgctgct tgcgctcggc
     2101 tcggccgcgt tcgggtcagc cagctgggcc gccctgagcg aagccgtcgc tcccgaacga
     2161 agcggctggc agctcggcct cgcgaacctg ggcacggctg gcgcggccgc ggcagcagga
     2221 ctattcggcg tggtaatcga cttggggaac gggttccggc ccgaagccgg ctacgtggtc
     2281 gctttcggcc tggctgcggt cgccgcactc gctggcggag cgctcgcctg gcgctggggc
     2341 agcctcagcc gcgccagggt cccgccagct caattcacga cccaaacctc agccaccgtg
     2401 gaggcacgct agatgctgca gcgcattcca attgacgtcc cgaccctgct gtcgcagctc
     2461 tacaagccac ttcaaccgat cgtccagcga attcattacg ttcgccgcgg cgaccggcgc
     2521 tacgagccgg ccgacatcct tcttcccagg ggctacgtcg cggaagttgt ctgccccggg
     2581 ttgacggcgc cggtgcatgc gtctttcggc ccggaaggcg agatgtacgt caccgaatct
     2641 ggccacaaga gctactcgcc gcctcgtgtg taccgagttg acactggaac tggcgaaaag
     2701 gagctcgtcg ctgagttcgg cggcgatcgc tggatcgaat ccggcgccgc cacgggcgcg
     2761 gtgtggcacg acggctcgct gtacgtcgcc aacaccgaca cgatcgtcaa gatcgacccg
     2821 accacgggtg cgatatccga cgtcgtgacc ggcctgcccg ggaagggcga ccaccaggcg
     2881 aaccacccgc tggtcggtcc tgagggcaag ctctactggg gccagggctg cgtcacgaac
     2941 atgggtgtgg tcggcgccga caacttcggc tacgaatggc tcccgatgtt cccggaaacc
     3001 tgcgatgtgc ccgctgagga catcgtcctc gcgggacgga actacgaagt cccgaacgtc
     3061 cttggcaacc ccctcgagaa ggccacgaca ggcgcgtacg tccccttcgg tactgataca
     3121 gacttcggcc agatcatccc cggcaacccc aaggcaagcg gcgcaatcct gcgtgtcgac
     3181 cctgatacga aggaagtgga ggccgtggca tgggggctgc gcaa
//
LOCUS       DATLCO010000223         5359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8377840, whole genome shotgun sequence.
ACCESSION   DATLCO010000223 DATLCO010000000
VERSION     DATLCO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8377840"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VMZ33_05370"
     CDS             <1..509
                     /locus_tag="VMZ33_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16693.1"
                     /translation="WGLTASVVYGLLLSTGILDSVSHRVVSLTLHQDLSAISIALVAL
                     HGAVLVLDRFVQTTVFQIAVPFATEYRPVWVGVGQLAFYVMAVVYASFYFRKRLGQRG
                     WRMLHYTTFLAFIGGAVHGLMAGTDTSAPWALWSYAASIAVVVFLLVYRIAMSVARGS
                     TRPAAERS"
     gene            516..830
                     /locus_tag="VMZ33_05375"
     CDS             516..830
                     /locus_tag="VMZ33_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUR16694.1"
                     /translation="MAYVIAEPCVDHMDQSCVQVCPVDAISSEAGVDRKMFIDPAGCI
                     ECGSCMSVCPNGAIYPENQVPVDWIDYVWLDAAWYRDRDMTRSVLAEVLEEGVRAAAG
                     AT"
     gene            827..1564
                     /locus_tag="VMZ33_05380"
     CDS             827..1564
                     /locus_tag="VMZ33_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUR16695.1"
                     /translation="MMSDNATIAVRHDFTESMVHLGIDVDEPLTSFRPGQYVSLGIPA
                     GEKLVQRPYSIVSWDAPTNRLELFIRRAPDGALTSRLWELGVGARVKVGPPKGLFVLD
                     PEDRRQRLLIGTGTGLAPLLAMLDLAYNRDDDVPTVLIHGVAYRDEAVYRNRVTAWQD
                     AGLPVDYLPTVSRPDDPRNGGWPGLSGRADVVLRRVLDEWPETRAGVAYMCGNPEMIS
                     SCSSVLADAGFAAADIRAEQFKPVARV"
     gene            1605..5339
                     /locus_tag="VMZ33_05385"
     CDS             1605..5339
                     /locus_tag="VMZ33_05385"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HUR16696.1"
                     /translation="MAIAELERDIESGRPRLTDDQRAAVEWGDGPLMVLAGAGTGKTT
                     VVVERVRHLLRMNPTLLPENVLVLTYNVRAAGELVDRLEEAVGLDIASRLWIYNFHSF
                     GNRVLSDHRTELGLAQNADVLDQVGQRLLIRELRPHFAHFVYHPVARDPNAGRGFADV
                     ISRAKDELVTPGEYAEFAQRRRGEFEKKYGDGSFAATVEELRKRELDGSMRSVKWVRS
                     KLVREGEESAEKEAGYHARKEVLGPAELVWWNKLTPEQERQAKGLLPTYIRDAQALDV
                     LRIAEEAEAYAIYQDELRNRGLLDYGEQQSRSIQLLTERPNILRRYQDQFRHVLVDEF
                     QDANMAQILLLELIGRGPDKPDNVVVVGDDDQSIYRFRGASYAAFEQFKQRFGQAPTW
                     APDRPTLEVATLPLLENRRSTPEILASANRLIQRNQKRLKKLPLTPIKAPGAKVEIVH
                     ATDEQDEADVVVSWIKKAYEELPQPRRWSDVAVLYRRHRHRDLIVDRLRKQDIPYVII
                     GGTGLFAVPEVRDAEAAMRVCANPDDSTAFVRLLSAGPWRLDAPEILRLTNAAAWDGR
                     PVYQAALHVLREGQIAVSEPVDAGLAARVSAKTKGGPTLWTEADFDEDEVESVRERRG
                     REERAKWRKTELDARLRVKLERLTTLLTELMPRAQRDGPFSVLEDFLVRTNTLHDLIA
                     VQTPESQRAVLALARFMRFVSDWQLAHQRESLRDFVEYLDLYQDVGGDLDMDQQGRID
                     VEGVQLMTIYQAKGLEYEAVVVPRLIEKEFPDSRDEQALIPIELLKQTPPDDFAIDEE
                     RRLLFVAMTRAKSRLLLTPLQPIGKKTMPSRFVGEIQTGDRPVVAPISSDEYVKLPPP
                     HDVHIEYRAMGPDAEIEASEPSASQTAAQSTVQLLKLMPLPLAHERRFYLRRRAVEIL
                     GNLEALGANDIPGKQVLIEDLMQVAREAAEEAEDARRNGLDPLTLNVLSRHAPAGKTI
                     LEMTALPPSFSHSQFYSYTECPLKYAFQRLYQIPVIDQPGYFEFGSLMHRAFEVYARA
                     RQEAIAAGHEPPGYEVLREAFQQEWRPRNFADEHAAQHYADRAEPALKRFYERELANQ
                     AQAVAFEAPFTMVIEPGNDEAPVRVYGRIDRIDRHADGTIEITDYKTGRSKEQKDVDI
                     DEQLSAYALATHMGAVFDPITRERLPAASKLTLYFTESDKSLSTTRTPEQLDQFRLSL
                     IEKARRIRSGDFTATPDMYRCGRCEFRLICPSRYGSDVAN"
BASE COUNT          953 a         1710 c         1770 g          926 t
ORIGIN      
        1 tctgggggct taccgcctcc gtcgtttacg ggctgctgct ctcgacagga attctcgact
       61 cggtctcgca ccgcgtcgtg agcctgacac tgcaccagga cctgagcgca atctcgatcg
      121 cgctcgtggc gctacacggc gccgtgctcg tgctcgacag gttcgtacag acgaccgtct
      181 tccagatcgc ggtccccttc gcgaccgagt accggccggt gtgggtcggc gtcggccagc
      241 tcgcgtttta cgtgatggcc gtcgtctacg cctcgttcta cttccgtaag cgcctcggcc
      301 agcgcggttg gcggatgctg cactacacga cgttcctggc gttcatcggt ggcgcggttc
      361 acggcctcat ggccggcacg gacacgagcg cgccgtgggc gctgtggagc tacgccgcct
      421 cgatcgcggt cgtggtgttc cttctggtct atcgcatcgc aatgagcgtg gcgcgcggct
      481 ctacccgccc cgccgcggag aggagctaac cagccatggc ctacgtcatc gccgaaccgt
      541 gtgtcgacca catggaccag tcgtgcgttc aggtctgtcc ggtcgacgcc atcagctccg
      601 aggctggtgt cgaccgcaag atgttcatag accctgcggg ctgcatcgaa tgtggatcgt
      661 gtatgagcgt gtgtcccaac ggtgcgatct atcccgagaa ccaggtgccg gtcgattgga
      721 tcgactacgt ctggctcgat gcggcgtggt atcgcgaccg cgacatgacg cgcagcgtcc
      781 tcgccgaggt tctcgaggag ggtgtccggg ctgccgctgg ggcgacatga tgtcggacaa
      841 cgcgacgatc gcggtccgcc atgacttcac ggagtccatg gtccaccttg gcatcgatgt
      901 cgatgaacca ttgaccagct tccggccggg gcaatacgtc tcgctcggga tcccggcggg
      961 cgagaagctc gtccagaggc cgtactcgat cgtgtcgtgg gacgcgccga ccaatcgact
     1021 ggagctgttc atccgtcgcg caccggatgg ggcgctcaca tcgcgcctat gggagctggg
     1081 ggtcggggcg cgcgtgaagg tcgggccgcc gaagggtctg ttcgttctgg atcctgagga
     1141 tcgtcggcag cggctgttga ttggcacagg caccgggctg gccccgctcc tggcgatgct
     1201 cgacctcgca tacaaccgcg atgacgatgt gcccaccgtc ttgattcacg gcgtggctta
     1261 ccgggatgag gccgtgtacc ggaatcgcgt cacggcctgg caggacgccg gcctgccggt
     1321 ggattacctg cccaccgttt cgcggccaga tgacccgcga aatggcggtt ggccggggct
     1381 gtcgggtcgt gccgatgtcg tcctgcggcg agtcctcgac gagtggccgg agacgcgcgc
     1441 aggagtggca tacatgtgcg gaaatccgga gatgatttcg tcgtgctcgt cggttctcgc
     1501 agacgcgggc tttgcggcgg ccgacattcg agcggagcaa ttcaagccgg ttgcgcgggt
     1561 gtgacaagca agctggcacg gaggggctct aggcttcggg ggagatggca attgcggaac
     1621 tggagcggga tatcgagagt ggtcggccgc ggctgacgga cgatcagcga gcggcagtcg
     1681 agtgggggga tgggccgctg atggtgctgg ccggcgccgg caccggcaag acgacagtcg
     1741 tggtggagcg tgtccgccac ctgctccgaa tgaacccgac cctgctgccg gagaacgtcc
     1801 ttgtcctcac gtacaacgtt cgcgcggcag gggagctggt tgatcggctg gaagaggccg
     1861 tcggcctgga tatcgcaagc cggctgtgga tctacaactt ccactcattc ggcaacaggg
     1921 tgctgtctga ccaccggacg gagctcggcc ttgcccagaa cgcggacgtg ctggaccagg
     1981 tcggtcagcg gctgctcatc cgcgagctcc ggccgcactt cgcgcacttc gtgtaccacc
     2041 ccgtcgcgcg cgaccccaac gctggccgcg gctttgccga cgtcatcagc cgcgccaagg
     2101 atgagcttgt aacgcccggg gagtacgccg agtttgccca gcggcggcgg ggcgagtttg
     2161 agaaaaaata cggcgacggc tcgttcgccg cgaccgtcga agagcttcgc aagagggagc
     2221 tcgatgggtc gatgcgcagc gtcaaatggg tccgcagcaa gctcgtgcgt gagggcgagg
     2281 agtctgccga gaaggaagcc gggtaccacg cacgcaaaga ggtgctcggc cccgctgagc
     2341 tcgtgtggtg gaacaagctg acgccagagc aggagcgcca ggcgaaaggg ttgctgccga
     2401 cgtacatccg cgacgctcag gcgctggacg tgctgcgcat cgccgaggag gcggaggcct
     2461 acgcgatcta ccaggacgag ctgcgcaacc gcggtctact cgattatggc gagcagcagt
     2521 cgcgcagcat ccagctgctc accgagcggc cgaacatcct gcgccgctac caggaccagt
     2581 tccgccacgt gctcgttgat gagttccagg atgcgaacat ggcccagatc cttctcctgg
     2641 agctgatcgg ccgcggaccc gacaagccgg acaacgtggt tgtcgtgggc gacgacgacc
     2701 agtccatcta ccgcttccgc ggcgccagct acgccgcgtt cgagcagttc aagcagcgct
     2761 tcgggcaagc tccgacgtgg gcacccgacc gaccaacgct ggaggttgcg accctgccgc
     2821 tcctcgaaaa ccgccgctca acgccggaga tcctcgcctc agcgaatcgg ctcatccagc
     2881 gcaaccagaa gcggctcaag aagctcccct tgacgccgat caaagcgccg ggcgccaagg
     2941 ttgagatcgt ccatgccacg gacgagcagg acgaagccga cgtggtcgtg tcctggatca
     3001 agaaggcgta tgaggagctt ccacagcctc ggcgctggag cgacgtcgcc gtcctctacc
     3061 gccggcatag gcatcgtgat ctcatcgttg atcggctgcg gaaacaggac atcccgtacg
     3121 tgatcatcgg cggcacgggc ttgttcgccg tccctgaggt tcgcgacgct gaggccgcca
     3181 tgcgcgtctg cgcgaaccca gacgacagca cggctttcgt gagactgctt tccgccggtc
     3241 cgtggcggct tgatgcgccg gagattctcc gacttacgaa cgcggcagcg tgggacggcc
     3301 ggcctgtgta ccaggccgcg ctccatgtcc tgcgtgaggg ccagatcgcc gtgtctgagc
     3361 cggtcgacgc agggctcgcc gcacgggttt ccgcgaagac caagggcggc ccgacgctct
     3421 ggacggaggc tgacttcgac gaagacgagg ttgagtcagt tcgcgagcga cgcgggcgtg
     3481 aggagcgcgc gaagtggcgg aagactgagc ttgatgcgcg tctgcgagtg aagcttgagc
     3541 ggctcaccac gctgctcact gagctcatgc cgcgggcaca gcgcgatggt cccttcagtg
     3601 tcctagagga cttcctggtc cggacaaaca ctttgcacga cctgatcgca gtgcagacgc
     3661 cggaatcgca gcgtgccgtg ctggcgttgg cacgcttcat gcgtttcgtg tccgactggc
     3721 agctcgctca tcagcgcgag agcctccgcg actttgtgga gtacctggac ctgtaccagg
     3781 acgtcggtgg cgacctggac atggaccagc agggccgcat cgacgtcgaa ggcgtccagc
     3841 tgatgaccat ttaccaggcg aagggccttg agtacgaggc ggtagtcgtg ccgcggctca
     3901 tcgagaagga attccctgac agtcgtgacg agcaggcgct gatacccatt gagttgctta
     3961 agcagacgcc gccggacgac tttgcgatcg acgaggagcg gcgtctcttg ttcgtggcca
     4021 tgacgcgtgc caagtcgagg ctcctgctca cgccgctcca gccgatcggc aagaagacca
     4081 tgcccagtcg gttcgtcgga gaaatacaga ccggtgatcg accggtagtg gcgccaatct
     4141 cctcggacga gtacgtgaag ctgccgccgc cccacgatgt ccacatcgag taccgcgcca
     4201 tgggtcctga cgctgagatt gaggccagcg agcccagcgc gtcacagacc gcggcacagt
     4261 cgacggtcca gctgctgaag ctcatgccct tgccattggc ccacgaacgg cgcttctatc
     4321 tgcgacgccg agccgtcgag atcctgggca acctcgaggc gcttggtgcg aacgacattc
     4381 ccggcaagca ggtgcttatc gaggatttga tgcaggtcgc ccgagaggcc gcggaagagg
     4441 ctgaggacgc gcgacgtaat ggactggacc ccctgacgct gaacgtcctg tcgcggcacg
     4501 ccccagcggg caagacgatc ctggagatga ccgccctgcc gccatccttc tcccactcgc
     4561 agttctactc gtacaccgag tgcccgctga agtacgcctt ccagcgcctg tatcagatac
     4621 ccgttatcga ccagccgggc tacttcgagt tcggttcgct catgcaccgc gcatttgagg
     4681 tctacgcccg agcgcgtcag gaagcaatcg cagccggtca cgaaccgccc ggctacgagg
     4741 tgctccgcga ggcgttccag caggagtggc gaccacgcaa ctttgcggac gagcacgctg
     4801 cgcagcacta cgcggaccga gccgagccag cgttgaagcg cttctacgag cgcgagctcg
     4861 ccaaccaggc gcaggcagtc gcgttcgagg cgccgttcac gatggtcatc gagcccggca
     4921 atgacgaggc gccggtccgc gtctacggcc gcatcgaccg catcgaccgt catgccgacg
     4981 ggaccatcga gatcactgac tacaagacag gccgatcaaa ggagcagaag gacgtcgaca
     5041 tcgacgagca gctgtcggcg tacgcgttgg ccacccacat gggcgcggtg ttcgacccga
     5101 taacaaggga acgtctcccc gcagcctcga agctgacgct ctacttcacc gagagtgaca
     5161 agtcgctcag cacgacgcgg accccggagc agctcgacca gttccgcctc tcgctcatcg
     5221 aaaaggcacg ccgcatccgc tcaggcgact tcaccgcaac cccggacatg tatcgctgtg
     5281 gccgctgcga gttccgattg atctgcccca gccgctacgg atcagacgtc gccaactagc
     5341 cgatgcggcc gcccgctgg
//
LOCUS       DATLCO010000224         3339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9872464, whole genome shotgun sequence.
ACCESSION   DATLCO010000224 DATLCO010000000
VERSION     DATLCO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9872464"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2260
                     /locus_tag="VMZ33_05390"
     CDS             <1..2260
                     /locus_tag="VMZ33_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16697.1"
                     /translation="SGLLDFIDHMLEWSRGFPIYIVTLARPELLERRPDWGAAKRSFS
                     SVYLEPLAEPDMRELLAGLAPGLPEQAVTAIVERADGMPLYAVETVRMLVAEGRLRED
                     DGIYLPVGDLTTLAVPESLTALISSRLDSLEAVDRSLIHDGAVLGQSFSIAALAAVSG
                     LSTADLEERLKGLVRRELLTRDMDPRSPEQGQYLFVQGLTREVAYNTLSRRDRKSRHL
                     AAARYFEQLGSDELASALAGHYLAAQHNAAEGPEADALAAQARIALRAAADRAVALGA
                     HDQAITFLEQALTVTTGAGEQAEMLEKAGHSAGVAVHPHKGEELLQRAIQLHRSTGNR
                     IGAARATAALGWVRSNDRQDQAALALLDEAATEFVDLWPDPVMIDLKLNLARALLQGD
                     DPRRALDVADQALEAAEHADLLPALARGLVAKGGAIGSLGRLREAIALIRAGEELARA
                     HELTDVLLIALVVGGYYLGEIDNAMALDNYREGLELARRTGQRGHMLQFVNNIGYTSF
                     LVGEWESGLAELDGALAQATDASSRVWLLSNALIIRASRGESIDDGLAEMDKLVAAHG
                     DPGLVSPTLDTRANLAQATGQTSEARTYWTRMGEKWSSEAAAGYYQGARHGIWSRDLK
                     SLRQDIAALDATGVHGRVVEVRRATLRAAEAALDGQTRDAIARYRDVLASWRDLRVVW
                     EEALAGIDMVTVLDPAEPEVSAAAASTRAILERLRARPYVERLDALLARQPAHVASRV
                     AEAPPRVAAESA"
     gene            complement(2268..>3339)
                     /locus_tag="VMZ33_05395"
     CDS             complement(2268..>3339)
                     /locus_tag="VMZ33_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUR16698.1"
                     /translation="SWLLAFILSPLVGLILRAFPRLPRVVVVGVVYGGLFIGLSAIIL
                     VVAGSLAASIGSFITDLPELQAKLPEILAGIQGGLNNLGFQVDLVAAAQNLFAGLGDL
                     GNDLVRPLTDLALFSLGIVGNLLIVIFLSLFIVLDKDKILAYTNRLIPPRYGETATLF
                     QTSVSSSFGGFIRGQAIQGLIYGLIAAGTHLAFGLEFLPASAALVGVLQTIPFFGPFL
                     SWAPPVVVAVLTKPEIAIPTLIVMAVGWFIVMNIVQPRVMATAVGIHPVVVLVSVLVG
                     LKIAGIAGAIFALPFAAVIAAFFHHFLSRNANLPRDVTTRAARRVGEREGRHVRVPKP
                     PALKPDAAAKPEVEPSAVEPSA"
BASE COUNT          574 a         1073 c         1175 g          517 t
ORIGIN      
        1 ctcagggctg ctggacttca tcgaccacat gctcgaatgg agccgcggct tcccgatcta
       61 catcgtcacc ctggcgcgcc cagaactgct cgagaggcgc cccgactggg gtgccgcgaa
      121 gcgcagtttc agttccgtct acctcgaacc gctggccgag cccgacatgc gcgagttgct
      181 ggccggcctt gctccagggc tgcccgagca ggcagtcacg gcgatcgtgg agcgcgccga
      241 tgggatgccc ctgtacgcgg tcgagacggt gcgcatgctc gtcgctgagg ggcgcctgcg
      301 cgaggacgac ggcatctacc tgccggtcgg tgacctcacg acattggccg tgccggaaag
      361 cctcaccgcg ctcatctcgt cgcggctcga cagtcttgag gcggtggatc ggtcgctcat
      421 ccacgacgga gcggtgctgg gccagtcgtt ttcgatcgcg gcgctggcgg ccgtatcggg
      481 cctgagcacc gccgatctcg aggaacggct caagggcctg gttcgccgtg agctcctcac
      541 ccgggatatg gacccgcgtt caccggagca gggccagtat ctcttcgtcc agggccttac
      601 ccgcgaggtt gcctacaaca cgttgtctcg gcgggatcgg aaatcgcgcc atctcgccgc
      661 ggccaggtac ttcgagcagt tggggtcgga tgagctcgcc agcgcgctcg ccggccatta
      721 ccttgccgcc cagcacaacg cggccgaggg gccggaggcc gatgccctcg ccgcgcaggc
      781 gcgcatagca ttgcgcgcgg ccgccgatcg agcggtcgcc cttggagcgc atgaccaggc
      841 gataacgttt ttggagcagg cactgaccgt cacgaccgga gcgggcgagc aggctgagat
      901 gctcgagaag gccggtcact cagctggcgt cgcggtccat ccccacaagg gcgaggagct
      961 gcttcaacga gcgatccagc tgcatcgctc gactggcaac aggataggtg cggcgcgggc
     1021 cacggctgcc ctcggctggg ttcgctcgaa tgaccgccag gaccaggcgg ccctggcgct
     1081 gctcgacgag gcggccaccg agttcgtcga cctgtggccc gatccggtga tgattgacct
     1141 caagctcaat ctcgctcggg cgcttttgca aggcgatgat ccgcgccgcg ccctcgacgt
     1201 cgcggaccag gccctcgaag ccgctgagca cgccgacctg ttgcccgcac tggcgcgcgg
     1261 gctcgtcgcg aagggtgggg caatcggcag cctcggccgg ctgcgcgagg cgatcgcact
     1321 gattcgagcg ggcgaggagc tggcccgcgc ccatgagctg accgacgtgc tgttaatcgc
     1381 gctggtcgtg ggcggctatt acctgggcga gatcgacaac gcgatggcac tcgacaatta
     1441 ccgcgagggc ctcgagctcg ccaggcggac cggccaacga ggccacatgc ttcagttcgt
     1501 caacaacatc ggctacacga gtttcctcgt tggcgagtgg gagtcgggcc tcgctgagct
     1561 ggatggggcg ctggcgcagg ccacggacgc ctcatcgcgg gtgtggttgc tcagcaacgc
     1621 gctgatcatc cgcgcgtcgc gcggtgaatc gatcgacgat ggtctggcgg agatggacaa
     1681 gctggtggcg gcgcacggcg accccggcct tgtctcgcca acactcgaca cgcgtgccaa
     1741 cctggcgcag gccaccggcc aaacgtcgga agccagaacg tactggacgc gcatggggga
     1801 aaagtggtcc agcgaagcgg cggcgggcta ctaccagggt gcgcgtcacg gaatctggtc
     1861 cagagacctg aaatctttgc gccaggacat cgccgcactc gacgccacag gcgtgcacgg
     1921 ccgggtggtg gaggtccggc gcgccacgct cagggcggca gaggcggccc tggatggtca
     1981 gacacgtgac gcgatcgcgc gctacagaga cgttctggcc agctggcgag atcttcgcgt
     2041 ggtgtgggag gaggctctgg ccggcatcga catggtcacc gttcttgatc ccgccgagcc
     2101 ggaggtgagc gcagctgccg catcgacgcg cgccatcctc gagcgtctgc gcgccaggcc
     2161 ctacgtcgag cgcctcgatg cgctgttggc ccggcagcca gcccatgtcg cgtcgcgcgt
     2221 ggcggaagcg ccgccgagag tcgcggccga gtctgcctag cggcgcgtca ggcgctcggt
     2281 tcgaccgcgc tcggttccac ttctggcttg gccgccgcgt cgggcttgag cgctgggggt
     2341 tttggcaccc tgacgtgtcg gccctcgcgt tcaccgacgc gtctggcggc gcgcgtcgtc
     2401 acgtctctcg gcagattggc gttcctgctc aggaagtggt ggaagaacgc cgcgatgacc
     2461 gcggcaaagg gcagggcaaa gatcgcgcct gcgatcccgg cgatcttgag accgacgagg
     2521 acggacacga gcaccacgac cggatggatg ccaacggccg tggccatgac gcgcggctgg
     2581 acgatgttca tgacgatgaa ccagccgacg gccatgacga tgagggtcgg gatcgcgatc
     2641 tctggcttgg tgaggacggc cacgacgacc ggcggcgccc acgacaggaa cggcccaaag
     2701 aatgggatgg tctgcaggac gcccacaagc gcggccgagg ctgggaggaa ctcgaggccg
     2761 aacgcgaggt gcgtccccgc ggcgatgagc ccgtaaatca agccctggat tgcctggccc
     2821 cggatgaacc cgccgaagct cgacgacacg ctcgtctgga agagggtcgc cgtctcgccg
     2881 tagcgcggcg ggatgaggcg gttcgtatag gcaaggatct tgtccttgtc gaggacgatg
     2941 aacagcgaca ggaagatcac gatcagtagg ttgccgacga tgcccaggct gaagagggcg
     3001 aggtcggtca gtggtctgac caggtcgttg cccaggtctc cgagacccgc gaaaaggttt
     3061 tgggcggcgg ccactagatc gacctggaag ccgagattgt tcaatccgcc ctggatgccg
     3121 gcgaggatct cggggagctt cgcctgcaat tcgggcaggt ccgtgatgaa gctgccgatc
     3181 gaggcggcga ggctgccggc gacgacgagg atgatcgccg acagtccgat gaacagaccg
     3241 ccgtagacaa cgccgacgac cacgacacgc ggcagccgcg gaaacgccct caggatcagc
     3301 ccgacgagcg gcgacaggat gaacgccagc agccacgac
//
LOCUS       DATLCO010000225         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_367183, whole genome shotgun sequence.
ACCESSION   DATLCO010000225 DATLCO010000000
VERSION     DATLCO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367183"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VMZ33_05400"
     CDS             <1..744
                     /locus_tag="VMZ33_05400"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HUR16699.1"
                     /translation="DDSAPTVATFESDVMYDYRSRRPTLEIMDGRPASLTWPDLSFRR
                     TRIADRDILVLSGAEPDFRWRELADDLASFARQLEVEQWISLGAIPAAVPHTRPVPVL
                     GTESSPGLLRGGVAPGPQGVLRVPSAAISVMDIALAEAGIPAVGYFAQIPHYISGDYA
                     PAAIALIEALARHLDIEIAVDALRAEAVVLRTRLDTATALDEKTRAYVERLEEMVDEA
                     RLPEGDELISEIERFLRERGSEPGHGQIH"
     gene            831..1571
                     /locus_tag="VMZ33_05405"
     CDS             831..1571
                     /locus_tag="VMZ33_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16700.1"
                     /translation="MNSISASPRPDSGAESRGRVLVVDDEAPITELLSTALRYMGYDV
                     ATARTGMAALESASRLPPDLVVLDVMLPDIDGFEVCRRLRSDGDFVPVIFLTARDAEE
                     DRVTGFIRGGDDYVTKPFSLEELTLRISALLRRTKMTGAGASDSNRLRYRDLEMDEDR
                     HQVWRDGSEVRLSPTEFRLLRYLLLNAERVLSKQQILDHVWQYDFNGDDNVVETYISY
                     LRRKLDSSHDKLIRTVRGFGYTLRAETS"
     gene            1582..>2870
                     /locus_tag="VMZ33_05410"
     CDS             1582..>2870
                     /locus_tag="VMZ33_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HUR16701.1"
                     /translation="MTLRARLLLSLGVLLAVALVVTGGLVVGLTRQSLVQQLDEDLRN
                     ASPADRSLGNGPGPGPDEPTGRRFALLVYGLDGEQLRTIPSGLANNPDPLPALPDGGI
                     SRLEMGQIIERPSLDGAVAYRLLAFRGRVEGGLDLNVIGVLAAPMTGVDQSVAVVIRT
                     LVVVGLVVLGAMLLIGTWIIRRGLRPLEQITATATRISGGDLSSRVGVRDDGSEVGRL
                     GNAFDGMLDQIQGAFASQAAALESKDRSESKLRQFVADASHELRTPLTTVRGYAELYG
                     AGGLSEEAALAQAMARIGTESRRMAALVEDLLLLARLDQGRPLRADPVLLSELVNDAV
                     ADARAVEPDRPVAAHVEDGVVVRGDEDRLRQVIGNLLGNVRMHTPAEVAVDVALSSRD
                     GVAALSVSDHGPGIDAEHAAQIFDRFYRVDSGRSRERG"
BASE COUNT          445 a          933 c          983 g          509 t
ORIGIN      
        1 gacgactcgg caccaaccgt ggccacattt gagtcggacg tgatgtacga ctaccgctcg
       61 cgccgcccga cgttggagat catggacggg cggccggcgt cattgacctg gcccgacctg
      121 tccttccgtc gaacccgcat cgccgaccgc gacatcctcg tgttgagtgg cgccgaaccc
      181 gactttcgct ggcgcgagct ggccgacgac ctggcctcgt tcgctcgaca gctggaggtt
      241 gagcagtgga tctcgctcgg cgcgataccc gcagcagtgc ctcatacgcg cccggtgccg
      301 gtcctgggga ccgagtcgtc accgggattg ctgcgcggcg gcgttgctcc gggcccgcag
      361 ggcgtgttgc gcgtgccctc ggccgcgatc tcagtgatgg acattgccct tgctgaggcc
      421 ggcatcccgg cagttggata cttcgcccag atcccgcact acatcagcgg cgactacgcg
      481 ccggcggcca ttgcgctgat cgaggcgctg gcgcgccacc tggatattga gatagcggtc
      541 gacgcgctcc gggcagaggc agtcgttttg cgcacgcgcc tcgacaccgc aacggctctt
      601 gatgagaaaa cccgagccta cgtggagcgt ctcgaggaga tggtcgacga ggcgcggctg
      661 cccgaaggtg acgagctgat cagcgagatc gagcgtttcc tgcgcgaacg cgggagcgaa
      721 ccgggccacg gccagatcca ctgacaccgc cacgggactg cgccagaagc caagcgtcct
      781 ctcggctaat tcacagcaac ctctaaggtt ccgggcgtgg aatacagcgc atgaattcga
      841 tctctgcgtc cccgcggccc gactcggggg ccgaatctcg cggacgcgtc ctcgtcgtcg
      901 acgacgaggc gcccatcacc gagcttctct ccaccgcact gcgctacatg ggttacgacg
      961 tcgcgacggc gcgcacgggc atggctgccc tcgagtccgc ctccaggctg ccacccgacc
     1021 tcgtcgtcct cgacgtgatg ctcccggaca tcgacggttt cgaggtatgt cggcgactgc
     1081 gctcggacgg cgacttcgtg ccagtcatat tcctcaccgc gcgcgacgct gaggaggatc
     1141 gtgtcaccgg cttcatccgc ggcggcgacg attacgtgac caagcccttt tctttggagg
     1201 agctgaccct ccggatcagt gcgctgctgc gccggacgaa gatgaccggc gcgggcgcgt
     1261 ccgacagcaa ccggcttcga taccgcgacc tggagatgga cgaagaccga caccaggtct
     1321 ggcgcgacgg ctcagaggta cgcctctccc cgaccgagtt ccgactgctc cgctacctgc
     1381 tcctgaacgc cgagcgggtg ctgtcaaagc agcagattct tgaccatgtg tggcagtacg
     1441 acttcaacgg cgacgacaac gtggttgaga cgtacatcag ctacctgcgg cggaagctcg
     1501 actcgagcca cgacaagttg atccggacag tgcgcgggtt cgggtacacg ctgcgagcgg
     1561 aaacctcctg acggacgcgc gatgacgctc cgcgctcggc tccttctatc tctcggggtc
     1621 ctcctggccg tcgcgcttgt agtcaccggc ggtctcgtcg tcggacttac gcgccagagt
     1681 ctcgtccagc agctcgacga ggatcttcgg aacgcaagcc ccgccgacag gtcgctcggg
     1741 aatgggccgg ggcccgggcc cgatgagccg actggtcggc gctttgcgct actcgtctac
     1801 ggactcgacg gcgaacagct gcgcaccatc ccgtcggggc tcgccaacaa tccggatccc
     1861 ctgcccgcgt tgcctgatgg cggaatttcg cgcctggaga tgggccagat catcgagcgg
     1921 ccgtcactcg acggtgccgt ggcttaccgg ctcctggcct tccgcggccg agtcgagggc
     1981 ggactcgacc tgaacgtgat tggcgtactc gccgcgccga tgacgggcgt cgaccagtcg
     2041 gtcgctgtcg tcattcgcac tctggtggtg gtggggctgg tggtgctcgg ggcgatgctg
     2101 ctcatcggga cctggatcat tcggcgcggc cttcgaccgc tggagcagat aaccgcgacg
     2161 gcaacacgga tttcgggggg cgacctttcg tcgcgcgtcg gtgttcgcga tgatggctcg
     2221 gaggtcgggc ggctgggcaa cgcgttcgac gggatgctcg accagatcca gggtgccttc
     2281 gcgtcacagg ccgcggcgct cgagtccaag gaccggtccg agtcgaagct gcgccagttc
     2341 gttgccgatg cctcgcacga gctgcggacg ccgttgacga cggtgcgtgg ctatgctgag
     2401 ctgtacggcg ccggcggctt gtccgaagaa gcggcgctcg cgcaggcgat ggcgcgcatc
     2461 gggacggaga gccggcggat ggctgctctt gtcgaggacc tgttgttgtt ggcgcggctc
     2521 gaccagggac ggccgcttcg ggcggatccc gtcttgctgt ctgagctcgt gaacgacgcg
     2581 gtggcagatg cacgggcagt agagccggat cggccggtcg ccgctcatgt tgaggatggt
     2641 gtcgtcgtgc ggggcgacga ggatcggctg cgccaggtga tcgggaacct gctgggcaac
     2701 gtgcggatgc acacccctgc agaggtggcc gtggacgtgg ctttgtcgtc gcgtgatggc
     2761 gttgcggcgt tgagcgtctc cgaccacggg ccgggtatcg acgccgagca cgccgcccag
     2821 atcttcgacc ggttctaccg cgtggactcg ggccgttcgc gcgagcgagg
//
LOCUS       DATLCO010000226         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4835711, whole genome shotgun sequence.
ACCESSION   DATLCO010000226 DATLCO010000000
VERSION     DATLCO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4835711"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1399)
                     /gene="coaBC"
                     /locus_tag="VMZ33_05415"
     CDS             complement(119..1399)
                     /gene="coaBC"
                     /locus_tag="VMZ33_05415"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HUR16702.1"
                     /translation="MSSNLEGRLILLGVTGSIAAYKSAELARLLTADGADVQALMTHT
                     AQAFLGPLTLQTLTRRRPLTDPLELLPDQRIAHIVAADTADALVVAPATARWIAAMAS
                     GLADDVITATALAAFPAAAVIVAPAMDGEMYSHPATRANVVKLQEFGYTIVEPEEGAL
                     ASGSVGRGRLADPALIVDALNRALEERPIRQPDLSLRPPVVMPQPAHDMVGLRVTVTA
                     GGTAEPIDPVRFIGNRSTGKMGVAIAEAAAARGADVTLIHGMTSVALPAGVELVAAPT
                     AAEMRNAVISAVAETDLLLMAAAVADFRPRNRADKKIARADGLTLQLEPTSDILAEAV
                     QVATGRGQGRPFVVGFAAETGSLERTREKAERKGVDLLVANDVAEEGSGFGTDTNRVT
                     IVVPGKTDEPWPLMTKRQVADRLLDRVLAELAAR"
     gene            complement(1396..>2191)
                     /locus_tag="VMZ33_05420"
     CDS             complement(1396..>2191)
                     /locus_tag="VMZ33_05420"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HUR16703.1"
                     /translation="MCLRIITDVLLALNVGNTNVNYAVIDDGNVGESGSMPTPSVADA
                     TSVLSELVDRVAPRQIVVASVVPAVSGALHDVAETRGIPLLIADETTIPIEIRVDSPG
                     GVGHDRLVNAYAALRIHGAPAIVVDMGTATTFDVVAADGAFIGGAIAAGAGLGLHALA
                     NGTAQLPHVPLVMPESAIGRDTVTAMQSGAVIGHSGLVLILIRAITEQLLAAGSPPPK
                     VILTGGLSSAEWARAIPSVDVIDPYLTLEGLARLHDEVGHPAGVQP"
BASE COUNT          338 a          736 c          751 g          366 t
ORIGIN      
        1 gccgtcagca tggagatgcg cttgccgtcg gcgcgcatct tggcgatgtc cgagacggtc
       61 acgcggcgga cgggcttctc cccgggcttg accggttcgg cgggttgatt ctgtgcgctc
      121 atctcgctgc caattctgcc agtacccggt ccaagagccg atctgcgacc tggcgcttgg
      181 tcatgagcgg ccacggctcg tctgttttgc cgggcacgac gatcgtgact cgattcgtgt
      241 ccgtgccaaa gcccgaaccc tcctcggcga cgtcattggc cacgagcagg tcgacgccct
      301 tgcgttccgc cttctcgcgc gtgcgctcca gcgaacctgt ttctgctgca aagccgacga
      361 caaagggccg accctgtccg cgccccgtcg cgacctggac ggcctcggcg aggatgtccg
      421 acgttggctc gagttgcagc gtcaggccgt cggcgcgggc gatcttcttg tccgcccggt
      481 tgcgcggccg gaagtcggca acggctgctg ccatgaggag caggtcagtt tcggccacgg
      541 ccgagatcac cgcgttgcgc atctcggcgg cggtgggcgc tgcgaccagc tccacccccg
      601 cgggcagtgc cacggacgtc atgccgtgga tcaaagtcac gtccgcgccg cgggcggcgg
      661 cggcctcagc gatcgcgaca cccatcttgc cggttgagcg gttgccgatg aagcgcacgg
      721 gatcgatcgg ctcggcggtt ccgcccgcgg taactgtcac acgtaggccg accatgtcat
      781 gggccggctg tggcatcacg accggaggcc gcagggacag atcgggctgc cttatcggcc
      841 gttcctccaa cgctcgattg agcgcgtcaa cgatcagcgc gggatcggcc agccgccccc
      901 tgcccacgga acctgacgcc agggcgcctt cctccggctc gacgatcgtg tagccaaact
      961 cctggagctt cacaacgttc gcgcgcgttg cggggtgcga gtacatctcg ccgtccatcg
     1021 ccggtgcgac gatcaccgcg gctgccggga acgcggcaag ggccgtggcc gtgatgacgt
     1081 cgtcggccaa gccggacgcc attgcggcaa tccaacgtgc cgtggcgggg gccacgacga
     1141 gcgcgtcggc cgtgtcggcg gcgacgatgt gcgcgatgcg ctggtcggga agaagctcga
     1201 gcgggtcggt cagcggccga cgtcgtgtca gcgtctgcag cgtcaacgga ccaaggaagg
     1261 cctgcgccgt gtgagtcatg agcgcctgga cgtcggcgcc gtccgcggtc agcaggcggg
     1321 ccagctcggc cgatttatag gccgcgatcg acccggtaac gccaagaagg atcagccggc
     1381 cctcgaggtt tgagctcacg gttgcacgcc ggccggatgg ccgacctcgt cgtgaagtcg
     1441 ggctagaccc tcgagcgtca agtaagggtc gatgacgtcc acgctgggga tcgcccgggc
     1501 ccattcggcg cttgacaggc caccggtcag gatcaccttc ggcggcgggc tgcccgcggc
     1561 aagcagctgc tcggtaatgg cccggatcag aatcaggacg agtccgctgt ggccaatcac
     1621 cgcgccgctc tgcatggccg tgacggtatc gcgtccgatg gcgctctcag gcatcaccaa
     1681 cggcacatgc ggcagctgcg ccgtcccgtt ggccagcgcg tgcagcccca ggccggcacc
     1741 ggccgcgatc gcaccgccga tgaaggctcc atccgcggcg accacgtcga acgtggtagc
     1801 agtgcccatg tcgacgacta tcgccggcgc cccgtggatg cgcagcgcgg cgtaggcgtt
     1861 gaccagccgg tcgtggccga ctccgcccgg actgtcgacg cgaatctcga tggggatcgt
     1921 ggtctcgtcg gcgatcaaca atgggatgcc gcgcgtctca gccacgtcgt gcaacgcgcc
     1981 gctcactgcc ggcactacgc tggcgacgac tatctgccgt ggagcgacgc gatcgacgag
     2041 ctcgctcagg actgaggtcg catccgcgac actgggcgtg ggcatgcttc ccgactcgcc
     2101 gacgtttccg tcgtcgatga cggcgtagtt gacgttcgtg ttgccgacgt tgagcgcaag
     2161 gagcacgtca gtgatgatac ggagacacat g
//
LOCUS       DATLCO010000227         3450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1858150, whole genome shotgun sequence.
ACCESSION   DATLCO010000227 DATLCO010000000
VERSION     DATLCO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858150"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VMZ33_05425"
     CDS             <1..910
                     /locus_tag="VMZ33_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUR16704.1"
                     /translation="TGAYVPFGTDTDFGQIIPGNPKASGAILRVDPDTKEVEAVAWGL
                     RNPFGLAFHPDGRLFATEHGSDERGARFIVGDPDDFYEIREGAWYGWPDFASGIRLDD
                     PRWGQGGMAREPALAEFPDPNPPMPTASFVTHAAANGFDFSRSSDFGFSGQAFVALFG
                     DLAPITTARQVVPAGFKVVRVDPDTGTITEFATNRIQGPASKLFHGGFERPSHCAFGP
                     DGNLYVVDFGEIKIAPEKGGIRMKQGTGTVWRIRRLPDTLAGTTPPRSMEVPFYPLQA
                     AAVAALAGIAVAGLVAVVRRLFGRSR"
     gene            complement(907..1533)
                     /locus_tag="VMZ33_05430"
     CDS             complement(907..1533)
                     /locus_tag="VMZ33_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HUR16705.1"
                     /translation="MTDHGPEHEHDHEHDHEHEGHEHEHQLDWASELEMLRESARHFY
                     EHQFDWRGHAPPEGFEGPKFYAADEQWRIEARLDSAVAGAGDPVQLATSTGQVRDMTI
                     AGDLVFDHGGGEHRLQAFRTHSHEGYESLFVPFRDATSGTETYGAGRYIDIPLEEDTD
                     GPIDLDFNFAYNPSCVFSPAYDCPYPPPRNKLSLAVRAGERMPFEKSG"
     gene            complement(1530..2018)
                     /gene="ybeY"
                     /locus_tag="VMZ33_05435"
     CDS             complement(1530..2018)
                     /gene="ybeY"
                     /locus_tag="VMZ33_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HUR16706.1"
                     /translation="MAAIYLPPWRIDVSTRPGVASPVPIRALARLAARALSVVGAPPP
                     ASLALILSDDTELATLNEQHMGHEGPTDVLSFPMLTPGFALPPGRRPHLGDIVISIER
                     AAEQASEGRGGQTSDMEWSAADELRLLVVHGVLHVCGHDHAEPGENEKMRALEKRILA
                     GQ"
     gene            complement(2026..>3450)
                     /locus_tag="VMZ33_05440"
     CDS             complement(2026..>3450)
                     /locus_tag="VMZ33_05440"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HUR16707.1"
                     /translation="RRGDRIDDVAREKLARYNLLEPRPDAVRAAGWFMLAGIAVVLLL
                     GWIWRFRPQLWHRNSALLLIALMLIAATVALKVTGDRSVLPYVVPTAAVGLLLAVLLD
                     AGAALMVTGLLALIGGAVIGSVEFAAFILFGGVAGIIVVRRGEKVTHFIQSAVAIAVV
                     NIGVITLFTMLGDRDFTGFFELAGASMASAAGAAVFALGSFVILGNAFGITTSFRLLE
                     LANPSQPLLRRLMFEAPGTYHHSLMVGNLAERAAEAIGADPLLARVAAYYHDVGKLQN
                     PQAFIENQTATENVHDDLEPLESVTLLKSHVAQGIDLAYEYRVPKPIIAFIPQHHGTA
                     LMSYFYARAQDQAQADIHALPNTPAAEEARATVESARFRHAGPKPQSREAAILMLADS
                     VEASVRSLKTPDEPQIRGMVSRIIQERQTDGQFDECDLTLRDLDRIREAFVAQLLGIY
                     HRRIEYPQNKIVELESRRSAGGSA"
BASE COUNT          549 a         1142 c         1182 g          577 t
ORIGIN      
        1 gacaggcgcg tacgtcccct tcggtactga tacagacttc ggccagatca tccccggcaa
       61 ccccaaggca agcggcgcaa tcctgcgtgt cgaccctgat acgaaggaag tggaggccgt
      121 ggcatggggg ctgcgcaacc cgttcggcct cgcgttccat cccgacggac gcctgtttgc
      181 gacggagcac ggctccgacg agcgcggggc tcggttcatc gtcggcgacc ccgacgactt
      241 ctacgagatc cgggagggtg cctggtacgg ctggcccgac ttcgcgagcg gaattcgtct
      301 ggacgatccc cggtggggcc agggtggcat ggcgcgcgag ccggccctcg ccgagttccc
      361 cgacccgaac ccgcccatgc ccaccgcctc attcgtcacg cacgcagccg ccaacggctt
      421 cgatttctca cggagttccg acttcggttt ctcgggacag gcgttcgtcg ccctgttcgg
      481 cgacctcgcg ccgatcacga ccgcacggca ggtcgttccg gcgggcttca aggtggtgcg
      541 ggtcgaccct gacaccggaa ccatcaccga gttcgccacc aaccgcatcc aggggcccgc
      601 ttcgaagcta ttccacggcg gcttcgaacg gccgagccac tgcgcgttcg gccccgacgg
      661 aaacctctat gtcgtggact tcggggagat caagatcgcg ccggagaagg gtggcatcag
      721 gatgaagcag ggcacgggca cggtatggcg catccgccgt ctgccggaca cgctcgccgg
      781 gacgactcca ccgcggtcca tggaagtgcc cttctatccg ctccaggccg cggccgtcgc
      841 tgcactggca ggcatcgccg ttgccggcct tgttgccgtg gtgcgccgac tgttcggcag
      901 gtcgcgctag cccgacttct cgaagggcat ccgctcgccc gcgcgcaccg cgagcgagag
      961 cttgttccgc ggcggcgggt aggggcagtc gtacgccgga ctgaagacgc acgaagggtt
     1021 gtaggcgaag ttgaagtcga ggtcgattgg tccgtccgta tcttcctcga gcggtatgtc
     1081 gatgtagcgc ccggcgccgt aggtctccgt ccctgaggtc gcgtcccgga acggcacgaa
     1141 cagcgactcg tagccctcgt ggctgtgcgt gcggaacgcc tgcagccgat gctcgccgcc
     1201 cccgtggtcg aaaacgaggt cgccggcgat cgtcatgtct cggacctgtc cggtcgatgt
     1261 cgcgagctgc accgggtcgc ccgcgccagc gaccgcagag tcgagccgtg cctcgattcg
     1321 ccactgctcg tcggccgcgt agaacttggg accttcgaac ccctcgggcg gggcgtggcc
     1381 gcgccagtcg aactggtgct cgtagaaatg ccgggcgctc tcgcgcagca tctcgagctc
     1441 cgacgcccag tcgagctggt gttcgtgctc gtggccctcg tgctcgtggt cgtgttcgtg
     1501 gtcgtgctcg tgctcgggcc cgtgatcggt cattgccctg caagtatccg cttctcgagg
     1561 gcgcgcatct tttcgttttc ccctggttcg gcgtgatcgt ggccgcaaac gtggagcacg
     1621 ccgtggacga cgagcaggcg cagctcgtca gcggcgctcc actccatgtc gctcgtctgg
     1681 ccgccgcgac cctccgaagc ctgctccgcg gcgcgctcga tggagatgac gatgtcgccg
     1741 agatgcggcc tacggccagg cggcagcgcg aaacccggcg tcagcatcgg gaatgacagg
     1801 acgtcggtcg ggccttcgtg gcccatgtgc tgctcgttga gcgtcgccag ctcagtgtcg
     1861 tcggaaagga tcagcgccag gctcgcgggc ggcggggcgc cgacaacgct cagggcacgc
     1921 gccgcgagtc gggccaaagc tcgaatgggc accggcgagg cgactcccgg tcgcgtcgag
     1981 acatctatgc gccacggcgg caggtagatc gccgccaccc gtgatctagg cagagccacc
     2041 ggcggaccgg cgcgactcca gctcgacgat cttgttctgc gggtactcga tgcgccggtg
     2101 gtagatgccc aggagctggg ccacgaacgc ctctcggatg cgatcgagat cgcgcagcgt
     2161 gaggtcgcac tcgtcgaact ggccgtcggt ctgtcgttcc tggatgatgc ggctgaccat
     2221 gccgcggatc tgcggctcat cgggcgtctt cagcgatcgg accgatgcct cgacgctgtc
     2281 ggcgagcatg aggatcgccg cctctctcga ctgcggcttg gggccggcgt ggcggaaccg
     2341 cgcgctttcg actgtcgctc gggcctcctc ggccgccggg gtgtttggca aggcgtggat
     2401 atcggcctgt gcctggtcct gagcgcgcgc gtagaagtag ctcatcagcg ccgtcccgtg
     2461 gtgctgcgga atgaaggcga tgatcggctt gggcacccgg tattcgtagg cgaggtcgat
     2521 gccctgggcg acatgcgatt tgagcagggt gaccgactca agcggctcaa ggtcgtcatg
     2581 cacgttctct gtcgccgtct ggttttcgat gaaagcctgg gggttctgga gcttgccgac
     2641 gtcgtggtag tacgccgcca cgcgcgccag gagcgggtcg gcgccaattg cctccgctgc
     2701 ccgctcggcg aggttgccga ccatcaggga gtggtggtac gtgcccggtg cctcgaacat
     2761 caatcggcgc agcaacggct gcgagggatt cgccaactcg aggagacgga aggacgtggt
     2821 gatgccgaag gcgttgccga ggatcacgaa cgaccccagg gcgaacactg cggccccggc
     2881 ggccgacgcc atgctcgcgc cggcgagctc gaagaagccc gtgaagtcgc ggtcgccaag
     2941 cattgtgaag agtgtgatca cgccgatatt cacgacggcg atggccacgg cggactggat
     3001 gaaatgcgtc accttctcgc cgcgccggac aacgatgatc ccggccactc ccccgaagag
     3061 gatgaacgcg gcgaactcga ctgagccgat cacggccccg ccgatgagcg ccagcaagcc
     3121 ggtgaccatc agcgctgccc ctgcatccag gaggacggcg agaaggaggc cgactgccgc
     3181 cgtgggcacg acgtacggga gcaccgagcg atcgccggtg accttcagcg ccacggtcgc
     3241 cgctatcagc atcagcgcga taagcagcag tgcgctgttg cgatgccaaa gctgcggacg
     3301 aaagcgccag atccagccga gcaacaggac cacggcgatc cccgccagca tgaaccagcc
     3361 agcggcgcgt acagcgtcgg gccgcggctc gagcaggttg tagcgcgcca gcttctcgcg
     3421 tgccacgtcg tcgatgcgat cgccgcggcg
//
LOCUS       DATLCO010000228         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5587151, whole genome shotgun sequence.
ACCESSION   DATLCO010000228 DATLCO010000000
VERSION     DATLCO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5587151"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="VMZ33_05445"
     CDS             complement(<1..1426)
                     /locus_tag="VMZ33_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR16708.1"
                     /translation="MAFIGRERELSALGAALMRAAEGEPSRIAVSGPLGIGISRLIDE
                     LGERLGGFAGVTLLRSRHHAPTSGVPYGALARALDDVLSTVPDDRLPQLIGSSAHDAI
                     SLLPDAAARVTALGLAVEAPPLDALEQRGARVRESLFGVIGRLATSGPLVLVVEDLEH
                     SDPGTRELLSVLVRIRRRLPLALIVGYHTDQLPRHHPALRFVREIEESAAVEKLEMQP
                     FVAEEMAAFVEAIQGERPTLGFVAAVMEGSRGNPLIAEQLVAAEAELAALRLSDPFEE
                     ILQARVEQLEPVGMRALRVLAAARRPLSAAELGAIQLRDGHLPRNVVSQVVESTLVRE
                     LESGIAIVHELCAEAIEASMLPTERHEVHAALATLVADRPAEAAWHWTQAGRAADAFA
                     SHLRAAREATNTESGSTAFFHYAAALELADGERPDDVEILTAAAEAAAAAGAFRRAAT
                     YIDQAIRRVAGGRVERLLDGRSARA"
     gene            1449..>2155
                     /locus_tag="VMZ33_05450"
     CDS             1449..>2155
                     /locus_tag="VMZ33_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HUR16709.1"
                     /translation="MGTGEVKKGFEASAGRPPLHPDNKLNELTGDEWLYFTKSLWTTA
                     YPSELGHAARKAHGANKPPRLMARLIEFFTKPGELVVDPFAGVGGTLLGAAVCRSPRR
                     ALGFEIEPRWAEVYESVAAKASAELDGNQFLLSDLGTSDPGGPRPFDRSRCQMVVGDA
                     VKLLAALEPASVDFVATDPPYNPQLKLTMAGGKLAEQFRNRRTDYAMVTADPDDIANS
                     ATYDEFLERMEAVFAELR"
BASE COUNT          364 a          727 c          727 g          337 t
ORIGIN      
        1 cagctcgagc gctgcgacca tcgagaaggc gctccacgcg gccaccagct acgcgacgga
       61 tggcctgatc gatgtacgtt gccgcgcgcc gaaacgcccc ggcggccgcc gctgcctcgg
      121 cggcggcggt gagtatctcg acgtcgtcgg ggcgttcacc atccgccagc tcaagggcag
      181 ccgcgtaatg aaagaacgct gtcgagcccg actccgtgtt ggtcgcctcc cgtgccgccc
      241 gcaggtggct tgcaaacgcg tcggcagccc ggcctgcctg cgtccagtgc catgctgcct
      301 ctgcaggtcg gtcggcaacc agggttgcca acgccgcgtg cacttcgtgc ctttccgtcg
      361 gcagcatcga cgcctcgatc gcctcagcgc acagctcatg gacgatggcg atgccagatt
      421 ccaactcgcg gacgagcgtc gattcgacga cctgactcac aacgttgcgc ggcaagtgcc
      481 catcgcgcag ctggattgcg ccgagttcgg ccgcgcttag cggccggcga gcggcggcga
      541 gcacacgcag agccctcatc cccaccggct cgagctgttc aacgcgcgcc tggaggattt
      601 cctcgaacgg gtcggaaagt cgcagggccg cgagctctgc ctcagcggcg acgagctgct
      661 ccgcgatcaa cgggttgccg cgcgagccct ccatcaccgc cgctacgaag cccagggtcg
      721 gccgctcgcc ctggattgcc tccacgaacg cggccatctc ctcggcgaca aagggctgca
      781 tctccagctt ctcaacggca gcgctctcct cgatctcgcg cacgaaacgc agcgccggat
      841 ggtggcgggg cagctggtcg gtgtggtagc caacgatcaa ggccagcggc aagcggcggc
      901 gaatgcgtac caggacgctg agcagctcac gcgtccccgg gtcgctgtgc tccaggtcct
      961 caacgacgag aaccagcgga ccggacgttg ccagccggcc gatgacgccg aaaaggctct
     1021 cgcggacacg ggcgccacgc tgctccagtg cgtcgagcgg cggagcttcg accgcaaggc
     1081 ccaatgcggt gacccgcgcg gctgcgtctg gcagcaggga aatcgcgtcg tgggcactgc
     1141 tcccgatgag ctgcggcagg cgatcatcag ggactgtgga cagcacatcg tcgagagcgc
     1201 gcgccagagc cccgtacgga acaccggagg tgggcgcgtg gtggcgcgac cgcagcaagg
     1261 taacgcccgc gaatccgccc agccgctcgc cgagctcgtc gatcaggcgc gatattccga
     1321 taccaagcgg gccggaaacg gcgatccgag agggctcccc ctcggcggcc cgcataagtg
     1381 cggcgccgag cgcgctcagc tcgcgttcac gaccgatgaa cgccattggc gaagcatagg
     1441 ctggtgaagt gggcactggt gaggtaaaga agggttttga ggcgtccgcg gggcggcctc
     1501 cgttgcaccc tgacaacaag ctcaatgagt tgactggcga cgagtggctc tacttcacga
     1561 agtcactgtg gacgacggcc tatccctcgg agctcggcca cgctgcgcgc aaggcgcacg
     1621 gcgccaacaa gccgccccgg ttgatggcac ggctgatcga gttcttcacg aagcccggtg
     1681 agctcgtcgt tgaccctttt gccggcgtcg gcggcacgct cctgggcgcc gcggtctgtc
     1741 gctcgccgcg tcgggctctg ggcttcgaga tcgagccgcg ttgggcggag gtctacgagt
     1801 cggtcgctgc gaaggcatcg gcagagctgg acgggaatca gttcctgctg tccgatctcg
     1861 gcacgtcgga tccgggcggc ccacgtccct tcgatcggtc gcgttgccaa atggtcgtcg
     1921 gtgacgccgt gaagctgctg gctgccctag agccggcgtc agtcgacttc gtcgctaccg
     1981 acccgcccta caacccgcag ttgaagctga cgatggccgg cgggaagctc gcggagcagt
     2041 tccgcaaccg acggacggac tacgcgatgg tcaccgcgga tcctgacgac atcgccaaca
     2101 gcgcgactta cgatgagttt ttggagcgga tggaggcggt gttcgccgag ctgcg
//
LOCUS       DATLCO010000229         2659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1117682, whole genome shotgun sequence.
ACCESSION   DATLCO010000229 DATLCO010000000
VERSION     DATLCO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117682"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VMZ33_05455"
     CDS             complement(<1..470)
                     /locus_tag="VMZ33_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16710.1"
                     /translation="MAQAYEYSISIPLSDGRVVIAAPRPEDKDRFDLLGERTLSIRSS
                     SSELDTQAHSFSSDITVDVEGHAMTLRLPTPADAQALRRALAAGAITATIVAAGAIAA
                     MQGNTGGSTDAGAQAPVRPDVNVSQPLQMPHARPDFENRKDAAVDDMLAAPAVQA"
     gene            complement(583..1512)
                     /locus_tag="VMZ33_05460"
     CDS             complement(583..1512)
                     /locus_tag="VMZ33_05460"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HUR16711.1"
                     /translation="MTFSIVARDPETGDFGVAVASKFLAAGSVVSHASAGVGAIATQA
                     LANYSYGRDGLDFLADGRSAQEAIDALTAADDGREQRQLGIVDAHGRAATYTGRECID
                     WAGGLTADGVAVQGNILAGSRVVDAMLAAYLTPGRPFPERLVDALIAADAEGGDRRGR
                     QSAAVLVKREKGGYGGNNDVYIDLRVDDDASPMQRLAGMLDLVHLYRDRPKPADLLPI
                     DEALVQELQSRLTAAGHAPTTLPKDGGLAKVLADTVTERTGEPREMPVAWDESWEAVM
                     AEWISTENLEERMTARGWIDPKVLEYLRKVSAG"
     gene            complement(1481..1930)
                     /locus_tag="VMZ33_05465"
     CDS             complement(1481..1930)
                     /locus_tag="VMZ33_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16712.1"
                     /translation="MPRENVNRATLAADREGDLHLHHPAELNEDANDFADNERVDLVE
                     ETIKPCAVIGGRQNEVGAQSSERILKLADPQGADASTLEIDDRRAGNTCRDRDIFLAH
                     PGAAPEDGVNSCQLCIAHGDRIERGPSLRLTRGLSWTRDLLHRCPRP"
BASE COUNT          405 a          876 c          880 g          498 t
ORIGIN      
        1 gcttgcaccg caggcgcggc cagcatgtcg tcgaccgcgg cgtccttccg attttcgaaa
       61 tcgggtcgcg cgtgcggcat ctggagcggc tgcgacacgt tgacgtcggg ccgaactgga
      121 gcctgggcgc cggcatcggt cgagccgccg gtgttgccct gcatagccgc gatcgcgccg
      181 gcggcgacga tcgtcgccgt gattgcgccc gctgccagtg cacgccggag cgcctgagcg
      241 tcggccggcg ttggcaggcg cagcgtcatc gcgtggcctt cgacgtcgac ggtgatgtcg
      301 ctgctgaagc tgtgagcctg ggtgtcgagc tcgctcgacg atgatctgat gctcagcgtt
      361 cgctcaccga gcagatcaaa acggtccttg tcctcaggac gcggcgccgc gatgacgact
      421 cggccgtcgc tcagcggtat ggagatcgag tactcgtagg cctgtgccat cggtctgttc
      481 tcccttcgtt tcctctgcgg ttctgtctgc cccctagcgg cttgagcgcg atgttccgcc
      541 cgcctggcgc atcagtcgac cgtccggccg acagacggag agtcagcctg ccgacacctt
      601 gcgcaggtac tcgaggacct tggggtcgat ccaaccgcgc gcagtcatgc gctcctccag
      661 gttctcggtt gagatccatt cggccatcac cgcctcccat gattcgtccc acgccaccgg
      721 catctcgcgc ggctcgccgg tgcgttcagt gacggtatct gcgagcacct ttgccaggcc
      781 gccgtccttc ggcagcgtcg taggtgcgtg accggccgcc gtgaggcggc tctggagctc
      841 ttgaaccagc gcctcgtcga tcggcagcag gtccgctggt ttggggcgat cgcgatagag
      901 gtggaccaga tcgagcatgc cggcgaggcg ctgcatcggg ctcgcgtcgt cgtccacgcg
      961 gagatcgatg tagacgtcgt tgtttccgcc gtagccgccc ttctctcgct tcacgagcac
     1021 ggctgccgac tggcggccgc gacggtcgcc gccctccgcg tccgcggcga tcagtgcgtc
     1081 gacgagtcgt tccgggaagg gccgccccgg cgttaggtaa gcggccagca tcgcgtcgac
     1141 cactctggac ccggcgagga tattgccttg gaccgcaacg ccgtccgccg ttaggccgcc
     1201 cgcccagtca atgcactccc tgcccgtata cgtagccgcc ctgccgtggg cgtcgacgat
     1261 ccccagttgg cgttgttcgc ggccgtcgtc cgctgccgtg agcgcgtcaa tggcttcctg
     1321 cgcggaccga ccgtctgcca gaaaatcgag cccatcacgg ccgtagctgt agttcgcgag
     1381 tgcttgtgtg gcgattgctc cgacgccggc actggcgtgg ctgaccaccg accccgcggc
     1441 caggaacttg gatgccacgg cgacgccgaa atcgccggtc tcagggtcgc gggcaacgat
     1501 ggagaaggtc acgcgtccac gataagccgc gcgtaagccg cagggaaggg ccccgctcga
     1561 tcctgtcgcc gtgcgctatg cacagctggc aagaattcac gccgtcctca ggcgccgcgc
     1621 cgggatgcgc caagaagatg tcgcgatcgc ggcaggtatt gcccgcacga cggtcgtcga
     1681 tctcgagcgt gctcgcatcg gcaccctgcg ggtccgccag cttcaggatt cgttcgctgc
     1741 tttgggcgcc aacctcgttc tgacggccac ctatgacggc gcacggcttg atcgtctcct
     1801 cgacgaggtc cacgcgctcg ttgtcggcga agtcgttcgc atcctcgttg agctcggctg
     1861 gatggtgaag gtggaggtca ccttcgcgat ccgcggcgag cgtggctcga ttgacgttct
     1921 cgcgtggcac ccaagagagt cggccttgct ggtggtcgaa gtaaagtcgg agctgccggg
     1981 tatcgatccg ttgctacgac ctctcgacgt caaggtgcgt ttggccccga cgatcgcgcg
     2041 agaccaattt ggctggcgcc caatcacggt gtcgcgcatc gccgtgctgc cagaggatcg
     2101 aacctcgcgg cgcctggtgg accgccattc tgtcgtccta gactcgagcc ttcctgcgcg
     2161 gtccagggaa gttcgcggct ggctcaagtg cccgacgcat gcgatagcag ggctctggtt
     2221 cctgacactt gttcgctcgg ccgagactag gcgaaacccc agcgccgtca ggcgagtaca
     2281 gcgtccacgg gtggctcgtg tccacgctca gagagtggct ggtgcgagcg tgaggacgcg
     2341 cacggtggat gctcggacac tgatcgaccc gcacagtgtc ggcagggacg acaattgacg
     2401 aaagacgctt caccaggtgg ggtccaagcc cgttggcaaa gccccctctg cgcttgacac
     2461 tccgtggcga cgtgactaac ctgttggcac tctccggcag agagtgctaa acccagatat
     2521 gaaggctccg cggtcgcgtg cgtgccgtaa aacccgagcg ccggcgcggt gggcctgaca
     2581 aggattcaac aggaggtaac gcgttggcga ctgccattgc gacgaagctt cggccgctcg
     2641 gcgaccgggt cgtcattca
//
LOCUS       DATLCO010000230         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6150023, whole genome shotgun sequence.
ACCESSION   DATLCO010000230 DATLCO010000000
VERSION     DATLCO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6150023"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..462)
                     /locus_tag="VMZ33_05470"
                     /pseudo
     CDS             complement(25..462)
                     /locus_tag="VMZ33_05470"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
     gene            complement(502..1083)
                     /locus_tag="VMZ33_05475"
     CDS             complement(502..1083)
                     /locus_tag="VMZ33_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HUR16713.1"
                     /translation="MKTRSGVSAVSAVVLMGLALVFAVAPVAAGTTLSESERVVKTAT
                     AQVGDRYLFASNGPNTFDCSGLIYFSFKQNGLADRIGGKRRTVKGYYDYFRARGKADK
                     VVGKPGDLIIWGRNAHMGIYLGNGMAVSTLVNPYGVKIHPVTGYIGMGVKAYLHIGLQ
                     RPGDPAPTPSTSPSPSPSASPTAPPTPTPTPAP"
     gene            complement(1083..1646)
                     /locus_tag="VMZ33_05480"
     CDS             complement(1083..1646)
                     /locus_tag="VMZ33_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HUR16714.1"
                     /translation="MSFVTRASRFVLLVSALICLVVSTTAPVAAGGTELDRVLSTARN
                     QIGDRYKHFGRGPDLFDCVGFVWYAFKQNNLQTQIGFYRGVAGYWDWFKARGLIAASN
                     PRVGDLIVWGQNTHIGIYLGPDQAISALVNPRGVSVHPIRKSWIGMGVKGYLRVNIQR
                     TPEEPLSVPPVTPSPTPTATPTTTPVV"
     gene            complement(1825..>2787)
                     /locus_tag="VMZ33_05485"
     CDS             complement(1825..>2787)
                     /locus_tag="VMZ33_05485"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HUR16715.1"
                     /translation="PPITAVGSDAPAIGQSLLLALVFLLLAAFPGQLLNKTIEENYTE
                     ISGWFARGGRLTQRARDVLSKFWQKRIGLGSFVVVSALLYGFLSPRFGPTLESVASLL
                     GIMAGLGIVVAAFELPLRFAYRSRLKDPGRLAVQPLTIFVGIACVVISRITDFQPGYL
                     YGLVVGYVFLKEMPIRDEGRANAATAVWMLAIALLAWLALPFVEDSLAAQPLLQTAFA
                     AGLATIFVGGLEGLLFELVPLRFLRGETVFAWNRALWALLFLAAAFTFVHILLTPTTG
                     YLGSTRTSPLLAAVILFVGFGIVSVAIWAYFKYRQAKHSQAVPG"
BASE COUNT          533 a          856 c          960 g          438 t
ORIGIN      
        1 gccgtgacac ctagcgtggc gcaatcaccc gctctgcaga ttccctcgga tcaggtcggc
       61 atggcggtca agatcggctc gtggtcgtgc tcggtcgaag acctcggcgg agatgatgaa
      121 gccgtcgccc ggaaaacgcg ggatcacgtg caggtggctg tgaaacacgt cctggccggc
      181 ggcctcgccg tcagcgagga aaagattgaa cccctctgaa tgcagatctg acccgtcgat
      241 tgccattgcg acgcgccggg cgacggacat caggtgccgc gccgtctcgt cgtcgaggtc
      301 gaggagcccg accgagtgct cgatcggcac caccagcgtg tgcccgggcg cgatgggatt
      361 gatgtccatg aacgcccaaa cgctgtcgtc ggagtacacg acgctggcgg gcgaccgccc
      421 agcaatgatc tcgcagaata tgcaggggtc cactgcccga gattgtcgct ccccgcgtcc
      481 cgcccggcag cgcgccgaac gctagggcgc gggtgtcggt gtcggtgtcg gcggcgctgt
      541 cgggctggcg ctcgggctcg gggatggcga cgtactcggc gtcggagcag gatccccagg
      601 gcgctggagg ccgatatgga ggtatgcctt cacgcccatg ccgatgtagc cggtcacggg
      661 gtggatcttc acgccgtagg ggttcaccag cgttgatacg gccatgccgt tgccgaggta
      721 gatgcccatg tgcgcattgc ggccccagat gatgagatcg cctggcttac cgacaacctt
      781 gtcagccttg ccgcgcgccc tgaagtagtc gtagtagccc ttgaccgtgc ggcgcttgcc
      841 cccgatccgg tcggccagcc cgttctgctt gaacgagaag tagatgagtc ctgagcagtc
      901 gaaggtgttt gggccgttcg aggcaaacaa gtagcggtcg ccaacctgcg ccgtcgccgt
      961 cttgaccacg cgctcggatt cgctgagcgt cgtaccggca gccacagggg cgaccgcgaa
     1021 gacgagggcg aggcccatga ggaccacggc ggagacggca ctgacgcccg aacgtgtctt
     1081 catcagacga cgggtgtggt cgttggcgtg gcggtcgggg tcggcgatgg tgtcacgggc
     1141 gggaccgaca gcggctcttc gggcgtccgc tggatgttca cgcggaggta gcccttgacg
     1201 cccatgccaa tccagctctt ccggatcggg tggacggaca cgccgcgcgg gttcacaagc
     1261 gcgctgatcg cctggtccgg cccgaggtag atgccgatgt gcgtgttctg gccccacacg
     1321 atgaggtcgc cgacgcgcgg gttggacgcc gcgatcaggc cacgcgcttt gaaccaatcc
     1381 cagtagccgg cgacgccgcg gtagaagccg atctgcgtct gcaggttgtt ctgcttgaag
     1441 gcgtaccaga cgaagccgac gcagtcgaag aggtcaggac cgcgaccgaa gtgcttgtag
     1501 cggtcgccga tctggtttcg tgctgtgctc agaacgcggt cgagctccgt cccgccagca
     1561 gccaccggcg ctgtggtcga aacgaccagg cagatgagtg cgctgacgag tagcacgaag
     1621 cgactggcac gcgtgacgaa ggacacgaac ttcctcccag ccgtgcctgg gggcacggat
     1681 ccggctgaac actcgtgacg ctggggcgcg gtcgagtgcc gttgcagaac cttaaccggg
     1741 cattacagaa gcgtgcaacc ttacgatcgg gtagagcact atggcccggg cgacgcagtg
     1801 ctcggaagca acgtccgcgg ggacctagcc cgggacggct tgactatgtt tcgcctggcg
     1861 gtacttgaag tacgcccaga tcgcgacgga cacgatgccg aagccgacga agaggatcac
     1921 agcggcgagc agcggcgagg tgcgggtgct gcccaggtag ccggtcgtcg gcgtgagcag
     1981 gatgtggacg aaggtgaacg ccgcggcgag gaagagcagc gcccacagtg cgcggttcca
     2041 ggcaaaaacg gtctcgccgc gaaggaatcg aagcggaacg agctcaaaaa gtagcccctc
     2101 gagtccgccg acgaaaatgg tcgcgaggcc cgccgcgaat gccgtttgca gaagcggctg
     2161 cgcggcgagt gagtcctcga cgaacggcaa cgcgagccac gccaggaggg cgatagccag
     2221 catccacacg gcggtcgcgg cattggcacg gccctcatcg cggataggca tctccttgag
     2281 gaatacgtat ccgacgacga ggccgtacag atagccaggc tggaaatccg tgatgcggga
     2341 gatcacaacg cacgcgatgc caacgaagat cgtaagcggc tgtaccgcca gccggcccgg
     2401 atccttcaac cggctccggt aggcgaaacg caacggcagc tcaaaggcgg cgacgacgat
     2461 tccgagcccg gccatgatcc cgaggaggct ggcgaccgat tcgagagttg gtccgaatcg
     2521 cggactcagg aagccgtaca gcagcgcgct gaccacaacg aaggacccga gcccgatccg
     2581 tttttgccag aatttcgaca gcacgtcacg cgccctctgc gtcagccggc cgccgcgcgc
     2641 aaaccaaccc gagatctctg tgtagttctc ctcgattgtc ttgttgagga gctgacccgg
     2701 gaaggcggcg agcaacagga acaccagcgc caggagcagg ctctggccaa tggcaggcgc
     2761 gtccgacccg acggctgtga tgggcgg
//
LOCUS       DATLCO010000231         7221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8384619, whole genome shotgun sequence.
ACCESSION   DATLCO010000231 DATLCO010000000
VERSION     DATLCO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8384619"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1063)
                     /locus_tag="VMZ33_05490"
     CDS             complement(59..1063)
                     /locus_tag="VMZ33_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HUR16716.1"
                     /translation="MGRRAALLTVVLALYGCSGAAVPTPSAVATSPPPLVDASPAASD
                     SPAPSLTDEPQTPSPLPPSIAPPTAAPSHDPTTTGEPAPTGVPESEFLTADTLVICLP
                     FDRSRFAEFDASGKAVGVDVEIAEQIAAQLGREPALVATLFDDLIGEIEARRCDITVG
                     GQFITHERLERIDMIPYRQGTPHVIVPAGNPLGIDELSDLCGRSFAVVGGTIYVDMVH
                     GQGDYAGAGLDASCVDGGAQPIDLREYDSQAAAEQALADAEVDAYAGNDFVTIDRPDV
                     YERTVALPLVRNGIGHFLGAQTVDADVRSALRAIIDDGTYADILSRYGVPDIALTVWP
                     "
     gene            complement(1092..3233)
                     /gene="purH"
                     /locus_tag="VMZ33_05495"
     CDS             complement(1092..3233)
                     /gene="purH"
                     /locus_tag="VMZ33_05495"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HUR16717.1"
                     /translation="MSGRIVVCVSGEGTNLRALHMYAGRGLLGGHIVKVVADRPCNAL
                     GFAADEGIATALIPVDQFRDRDAWDVAFAAALAEEEPDLVVLAGFLRWIGPATLEKFR
                     GRILNVHPSLLPAFPGRESIRDALSAGVKVTGVTVHVVDQTRDGGPILAQEAVGIRAE
                     DSEEGLLARVHGVEHRLLPRVVASMLSDITEAALPKPRRALISVSDKSGLPAFARALV
                     GLGFELVSTGGTAKALRAAGLEVTDVAEVTAFPEMLDGRVKTLHPRIAGGVLADWRNP
                     DHRQQLAAAGIEPFELIVVNLYPFAEAAARRDSTEDELIEQIDIGGPTLVRAAAKNHA
                     NVGIVTDPAQYDPVLLELGHGGGLTTDMRRELAMVAFRLTSGYDALIAEELSRRWWHR
                     VEMPGRLTVELNLVEQLRYGENPHQSAGLYRATGVDLARGAFAEGPTLLQGKQLSYNN
                     ILDTAAATGLARDLRGPACAIVKHANPCGVAEADDIDAAWDLALAGDPVSAFGGVVAV
                     TRAIDASLAERLTSIFLEVVATPDVLPDALPILGRKPNLRVLIDPALGSPPGKGLELR
                     SAGGAMLVTDADVAADDAATWTVATQRSPTDAEKEALDLAWRVCRHVKSNAIVLVKDN
                     AVVGIGAGQMSRVDSARLAVAKAGVERANGAVCASDAFYPFPDALEVCAAAGVTAFVQ
                     PGGSQRDAEVIGAADAAGATMLVTGVRHFRH"
     gene            complement(3230..4246)
                     /gene="purM"
                     /locus_tag="VMZ33_05500"
     CDS             complement(3230..4246)
                     /gene="purM"
                     /locus_tag="VMZ33_05500"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HUR16718.1"
                     /translation="MRASRTAYADAGVDVEAGDRAVDLMRERIPRGAGDLLGLGSFAA
                     ALELPEGFRHPLLVTSTDGVGTKTEIARRLGRLDTVGQDLVAMCADDVVCHGASPAYF
                     LDYLAVGRVDPERVATLVGGVSDACAEISCSLVGGETAEHPDLMGDDEFDIAGFCIGF
                     VERDQLIDGSDARTGDVVIGLASSGLHANGYSLVRRLVDDGRLPLDETLLTPTKLYAN
                     AVLSLLATLRDSAVRVGGLAHVTGGGLPANLPRAVDSSLGIRLQVGTWSLPDVFGRVQ
                     SAAGIGDSEMRATFNCGIGFAVVVQPDAGDATIDILAREGVEAWTIGEVRPADDLGAR
                     YVEA"
     gene            complement(4243..5646)
                     /gene="purF"
                     /locus_tag="VMZ33_05505"
     CDS             complement(4243..5646)
                     /gene="purF"
                     /locus_tag="VMZ33_05505"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HUR16719.1"
                     /translation="MVGVASPTQEAASLAALGIFALQHRGQESAGIAVSDGKDVMVYK
                     DLGLVDQVIDERRLESLRGHLAIAHCRYSTTGSTIWENSQPTFRLGPRRAVAVGHNGN
                     LVNTQALLDELPGGRSRLVGTTDTELLTALLATEPGADVVEALVGLLPRLSGAYSLVV
                     MDEGRVVGARDPYGFRPLVMGVIPSADGETWCLASETAALDVLGAEYVRDVEPGELVV
                     LGEPGGPRSVRFAEGKEQLCVFELIYFARPDSYMLGRNLYEVRRRMGEELAREAPAEA
                     DLVMPVPETGAPAAAGYAEASGIPYREGMTRNRYAGRTFIQPSNGLRQRGVTVKLSVL
                     REQVRDRRLIVVDDSIVRGTTTRQIVGLLRRAGAREVHVRISAPPIFHPCFYGIDTQI
                     ETELIAGSKSVEEIREFIGADSLAYLSIGGVLDAVDLPRDRFCFACFDGKYPVPVPYD
                     AGNRKFLLEREPLTLVR"
     gene            complement(5656..>7221)
                     /locus_tag="VMZ33_05510"
     CDS             complement(5656..>7221)
                     /locus_tag="VMZ33_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HUR16720.1"
                     /translation="PFAGKLLIEASLELVERGLVDGLQDLGAAGLTCATSEMADRGGT
                     GIRIDLDSVPLREPNMKPFEVLISESQERMLAEVAPENLDAVLQVCRRWGLPSAVVGV
                     VTDDGDITVVEGGREIARVPASALASQSIEMSRLAAPPQRRRAAPAPGDTAMPHDGLP
                     ERGMDPGMVLEALMGHANLASRSWVTTQYDQTVGADTVEGCEHAAAVIRVKGTRKALV
                     VATDSQPAAALHDPSLAAALAVAEATRNVAVTGARLLGITNCLNFGDPTVPEAFWQLS
                     ESVRGMGDACRALGVPITGGNVSLYNESPLGRIAPTAQIGAVGLLDDLDLLIRPQFCN
                     EGDLIVLLGEIASGMAGSAYERLAGAAPDDRPPQLDLAREANLQRLLQQAAQDGLLHS
                     AHDVSGGGLAVAVAEAAIWSGLGGDLQLAVAMAPAVALFGESPTRAIVTVAPERWNEL
                     GRLASGLRIACRRLGTVGGDRLRIRLVGVGATGAAEERGAGVADAVDVSLAELRRAWE
                     RGLPRALGDEVRV"
BASE COUNT         1200 a         2500 c         2423 g         1098 t
ORIGIN      
        1 tgcccccaat ggctctgact ggtctcgttc tcgttcatcg ggagattcta gtccccagtc
       61 aaggccagac cgtcagcgcg atatcgggca cgccgtaccg gctcaggatg tccgcgtatg
      121 tgccgtcgtc gatgatcgcc cgcaaagcgc ttctaacgtc cgcgtcgact gtctgcgcgc
      181 ccaggaagtg gccgatgccg tttcgaacga gcggcagcgc cacggtgcgc tcataaacgt
      241 ccggccggtc gatcgtcacg aagtcgttgc cggcatacgc atcaacctcc gcatctgcga
      301 gggcctgctc ggccgcagcc tggctgtcgt actctcgaag atcgatcggt tgcgccccac
      361 cgtcgacaca gcttgcgtcg agaccggcac cggcgtagtc cccctggcca tggaccatgt
      421 cgacatagat cgtgccgccg accactgcga agctccgtcc gcaaagatcg ctcagctcgt
      481 cgatgcccag tggattgccc gccggcacga tgacgtgcgg tgtgccctgt cggtacggga
      541 tcatgtcgat gcgctcaagg cgctcatggg tgatgaactg gccaccgacg gtaatgtcgc
      601 aacgtcgcgc ctcgatctcc ccgatcaggt catcgaacag ggttgcgacc agagctggct
      661 cgcgccccag ctgcgccgcg atttgctccg cgatctcaac gtcgactccg accgctttcc
      721 cactcgcgtc gaattccgca aaccgactgc ggtcgaaagg caggcagatg acgagcgtat
      781 ccgcggtgag gaactcgctt tcggggacgc ctgtcggcgc aggctcgccg gtcgtcgttg
      841 ggtcgtgact cggcgctgcc gtaggtgggg cgatcgaagg tggaaggggt gacggcgtct
      901 gcggctcgtc ggtgagggat ggagcgggag agtcactggc tgccgggctg gcgtcgacca
      961 gcggtggcgg gctggtcgcg acagcggagg gggtgggaac tgccgcgcca ctacacccgt
     1021 acagcgccag aacgactgtc aggagtgcgg cacgacggcc catgccgcga tggtacgagc
     1081 ttgtgcaatg cctaatggcg gaagtgtcga actccagtta cgagcatggt cgcccctgcc
     1141 gcgtcagccg cgccgatgac ctccgcatct cgctgcgatc cgcctggctg cacgaaggcc
     1201 gtcacgccgg ccgcggcgca gacctcgagt gcgtcgggaa acgggtagaa ggcgtccgat
     1261 gcgcagacgg ccccattggc gcgctcaacg ccggctttgg cgacggccag gcgggcgctg
     1321 tcgacccggc tcatctgtcc agcgccaatc ccgaccacgg cgttgtcctt caccagcacg
     1381 atcgcattcg atttgacgtg tcggcagaca cgccaggcca ggtcgagcgc ctctttctcg
     1441 gcgtcagtcg gggaccgctg cgtggcgacg gtccaggtcg ccgcatcgtc ggcggcgaca
     1501 tctgcgtcgg ttaccagcat cgctccgccc gcgctgcgca actccagccc cttgcctggc
     1561 ggtgacccga gcgccgggtc gatgagcacc ctcaggttcg gctttcggcc gaggatggga
     1621 agggcgtcag gcaggacatc cggcgtggcc acgacctcga gaaagatgct ggtcaggcgc
     1681 tcggcgagtg aggcatcgat ggctcgagtc acggcaacga cgccgccgaa cgcgctgaca
     1741 gggtcgcctg caagtgccag gtcccacgct gcgtcgatgt cgtccgcctc ggcgacaccg
     1801 cacggattgg cgtgcttgac gatggcgcag gccggtccgc gcagatcgcg cgccaggccc
     1861 gtcgcagcgg ccgtgtcgag aatgttgttg tacgagagtt gcttgccctg gagcagcgtg
     1921 gggccctccg cgaaggcacc gcgggccaga tcaacgcccg tcgcgcgata aaggcccgcg
     1981 gattggtgcg ggttctcccc gtagcgaagc tgctccacga ggttgagctc gactgtcagg
     2041 cggcccggca tctcaacgcg gtgccaccat cggcgcgaca gctcctcagc gatcagcgcg
     2101 tcatagccgc tcgtcaggcg gaacgcgacc atggccagct cgcgccgcat gtcagtggtc
     2161 agaccgcccc cgtgaccgag ctccagcaga accgggtcgt actgagcggg atccgtgacg
     2221 atgcccacgt tggcatggtt cttggcggcg gcgcgcacga gagtgggccc gccgatgtcg
     2281 atctgctcga tgagctcgtc ctcagtcgag tccctgcgcg ccgcggcttc ggcgaacggg
     2341 tagaggttga ccacgatcaa ttcgaaaggc tcgatgccag ccgccgccag ctgctggcgg
     2401 tggtcggggt tgcgccaatc ggccagcaca ccgccggcga ttcgcggatg cagggtcttg
     2461 acccggccgt cgagcatctc tgggaatgcc gtgacctcgg caacgtcagt gacctccagg
     2521 cccgcggctc gcagcgcctt ggccgtgccg cccgttgaga caagctcgaa gccaaggcca
     2581 accaaagcgc gggcgaacgc cggtagcccg gatttgtccg agaccgatat cagcgcccgt
     2641 cgcggcttcg ggagcgcagc ctccgtgata tccgagagca tggaagcgac gacgcgcggc
     2701 agcagccggt gctcaacgcc gtgcactcgc gcgaggaggc cttcctccga gtcttcggcg
     2761 cgtatcccaa cagcctcctg ggcaagtatc ggcccgccgt cgcgcgtctg atcgacgacg
     2821 tgaaccgtca cgccggtcac cttcacgccg gcggagaggg catcgcggat cgactcgcgg
     2881 ccggggaaag ccggcaagag gctgggatgg acgttcagga tgcggccgcg gaacttctcg
     2941 agcgtggccg ggccgatcca gcggaggaag ccggccagaa ccacgaggtc gggctcttcc
     3001 tcagcgaggg cggcggcaaa ggcgacatcc catgcgtccc gatcccggaa ctggtcgacg
     3061 gggatcagcg ccgtggcgat cccctcgtcg gccgcgaagc ccagcgcgtt gcagggtcga
     3121 tcagcgacga ccttgacgat gtggcctccc agcagtccgc gaccggcgta catgtgcagc
     3181 gcccgaaggt tggtcccctc ccccgagacg cacacgacta tccgcccgct cacgcctcca
     3241 cgtatctcgc gcccagatcg tccgccggcc gcacctcgcc gatcgtccac gcctcgacgc
     3301 cttcccgagc caggatgtcg atcgtggcat cgcccgcgtc gggctgtacg acgacggcaa
     3361 aaccgatgcc gcagttgaac gtcgctcgca tctcgctatc gccgatgcca gctgcgctct
     3421 ggacgcgccc gaacacgtcg ggcaaggacc aggtgccgac ctggagccgt atgccgaggc
     3481 tgctgtcaac ggcgcgcggc aggttcgctg gcaatccccc gccggtgacg tgcgctagac
     3541 cgccgactcg aacggcggag tccctcagcg tcgcgagcag gctgagcacg gcattcgcat
     3601 acagcttggt cggcgtcagc agcgtctcgt cgagcggcag gcgcccgtcg tcgaccagcc
     3661 gccgcaccag tgagtaaccg tttgcgtgga ggccgctgct cgccaggccg atgacgacat
     3721 cgccggtgcg agcgtcagac ccgtcgatga gctgatcccg ctcgacgaac ccgatgcaga
     3781 aaccggcgat gtcgaattcg tcgtcgccca tcagatcggg gtgctccgcc gtctcgccgc
     3841 cgacgagcga gcacgagatc tcggcacatg catcggacac ccccccgaca agcgtggcca
     3901 cccgctccgg atcgacgcgg cccacggcca ggtaatcgag gaagtaggcg ggcgacgccc
     3961 cgtggcagac gacatcgtcc gcacacatag cgaccaggtc ctggccgacg gtgtcgagcc
     4021 tgcccagccg tcgggcgatc tccgtcttcg tgccgacgcc atcagtcgag gtgacgagta
     4081 gtgggtggcg aaatccctct ggcaactcga gcgcggcagc aaacgagccg agacctagca
     4141 ggtcaccggc gcccctaggg atccgctctc gcatcaggtc gaccgcccga tcacctgcct
     4201 cgacgtctac cccggcgtcg gcataggcag tccgcgaggc tctcatcgga cgagcgtgag
     4261 tggctcgcgc tcgagcagga acttgcgatt gccggcgtcg tacgggaccg gcaccgggta
     4321 cttgccgtcg aagcaggcga agcagaagcg gtccctgggc aggtcaactg cgtccagcac
     4381 gccgccgatt gacagatacg caagcgaatc ggccccgatg aactcgcgga tctcctcaac
     4441 ggactttgac ccggcgatca gctccgtctc gatctgggtg tcgatgccgt agaagcacgg
     4501 gtggaagatc ggcggcgcgc tgattcggac atggacttcc cgggcgccgg cgcgacggag
     4561 aagcccgacg atctgacggg tggtggtccc gcgcacgatc gagtcgtcga ccacgatcag
     4621 gcgtcggtca cgaacctgct cgcgtagcac cgacagcttg acggtcacac cccgctggcg
     4681 cagcccgttg gacggctgga tgaacgtccg tccggcgtag cggttgcgcg tcatgccttc
     4741 gcggtacgga atgcccgacg cctctgcata tccggcagcg gcgggcgccc ctgtctccgg
     4801 gactggcatc accaggtcag cctcggccgg tgcctcgcgc gccagctcct cgcccatccg
     4861 ccggcgcact tcgtaaaggt tccgaccgag catgtacgag tcgggccggg cgaagtagat
     4921 cagctcgaaa acgcagagct gctccttccc ctcggcaaag cgcacggacc gcggcccgcc
     4981 gggctcaccc aggacaacga gctcgcccgg ttccacgtcg cgcacgtatt cggcgccaag
     5041 gacgtcgaga gcagccgtct cagaagccag gcaccaggtc tcgccgtcag cggacgggat
     5101 cacgcccatg accagcggcc ggaaaccgta cggatcgcgc gcgccaacga cccgcccctc
     5161 gtccatgacg accagcgaat aggcgccgct cagccgcggc aatagcccga ccagcgcctc
     5221 aacaacgtcc gcgcccggct ctgtggcgag gagcgccgtc agcagctcgg tgtcggtcgt
     5281 gcccaccagt cgtgatcggc cgcccggaag ctcgtcgagc agggcctgcg tgttgacgag
     5341 gttgccgttg tgtccaacgg cgactgcgcg tcgcgggcca agccgaaacg tcggctggct
     5401 gttttcccag atggtggagc ccgtcgtgga atagcggcaa tgcgcgatgg ccaggtggcc
     5461 gcgcagcgac tcaaggcgcc gctcgtcgat cacctggtcg acgaggccga ggtccttgta
     5521 gaccatcacg tccttgccgt cgctgacggc gatacccgcg gactcctggc cgcgatgttg
     5581 gagggcgaag atcccgagcg cggccagcga ggcggcctcc tgagtcggcg aagcgacccc
     5641 gaccacgccg cacatctaga cgcgcacctc gtcgccgaga gcgcgcggca ggccacgctc
     5701 ccaggcacgg cgaagctcgg cgagcgagac gtcaaccgcg tccgcgacgc cagccccacg
     5761 ttcctcagcc gcacctgtcg cgccgacgcc gaccagccgg atccgcagcc gatcgccacc
     5821 aacggtgccg aggcggcggc acgcgatacg caggccggat gccagccggc cgagctcgtt
     5881 ccatcgctcg ggcgccacgg tcacgatggc gcgcgtcggc gattcgccga acaacgcgac
     5941 ggccggcgcc atggccacgg cgagctgcag gtcgccgccc agtccgctcc agatcgcagc
     6001 ctcagccacg gcgacggcaa ggccgccacc cgatacgtca tgcgccgagt gaaggagccc
     6061 atcctgcgcg gcctgctgga gcagtcgctg gaggttggcc tcgcgcgcca ggtcgagctg
     6121 cggcgggcgg tcgtccgggg cggcaccggc gaggcgctca taggcagagc cggccatgcc
     6181 cgacgcgatc tcaccgagca aaacgatcag atcgccctcg ttgcagaact gcggccggat
     6241 aagcaggtcc aggtcgtcga gcagcccgac ggcgccgatc tgcgcggttg gtgcgatacg
     6301 accgagtggc gattcgttgt agaggctgac gttgccgccc gtgatcggga cgccgagtgc
     6361 ccggcaagcg tcccccatgc cacgcaccga ctcggacagc tgccagaacg cctcgggcac
     6421 tgtggggtcc ccgaagttga ggcagttcgt gatgcccagc agccgcgcac cggtaaccgc
     6481 gacgttgcgc gtggcctcgg ctactgccag tgcggcggcg agcgacggat cgtggagggc
     6541 ggcggcgggt tgcgagtctg tcgcgacgac gagcgccttg cgggtgccct tgacccggat
     6601 cacggcagcc gcgtgttcgc agccctccac cgtgtcggcg ccaactgtct gatcgtactg
     6661 agtcgtgacc cacgagcggc tcgcaaggtt ggcgtggccc atgagcgctt ccagaaccat
     6721 tccggggtcc atgccccgct ccgggaggcc atcgtggggc attgccgtgt ctccgggcgc
     6781 cggcgctgcg cgacgacgct gcggcggggc ggcgagccga ctcatctcga tggactggct
     6841 ggccaatgcg ctcgcgggga cgcgcgcaat ctcgcgcccg ccctcgacga cggttatgtc
     6901 accgtcgtcc gttaccacgc cgacgaccgc cgaaggcagg ccccagcgcc ggcacacctg
     6961 gagcacggcg tcgaggttct caggcgctac ctcggcgagc atccgctcct gtgactcgct
     7021 gatcagaacc tcaaatggct tcatattcgg ctcgcgcagc ggaacggaat cgaggtcgat
     7081 gcggatgccg gtgccgccgc gatcggccat ctcgctggtg gcgcacgtca gcccagcggc
     7141 gccgagatcc tggaggccgt caaccagccc acgctcaacg agctcgagcg aggcctcgat
     7201 gagcagcttg cctgcgaacg g
//
LOCUS       DATLCO010000232         8561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6150712, whole genome shotgun sequence.
ACCESSION   DATLCO010000232 DATLCO010000000
VERSION     DATLCO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6150712"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VMZ33_05515"
     CDS             complement(<1..516)
                     /locus_tag="VMZ33_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR16721.1"
                     /translation="MNNAYRIAGQLVLYDSIVGGVVTVVDGLIVAIEEREVPDTANFI
                     APGFVDVHVHGWGGHDAMGGRAALDGMARALARRGVTSFLPTAVTAPIEALGQFAENV
                     RSWMAAGGATDGAQPLGFNLEGPFLAAERRGAHDSTQLRIPADVDDATLEPLLQGLRL
                     TTVAPELPGALE"
     gene            complement(513..1430)
                     /locus_tag="VMZ33_05520"
     CDS             complement(513..1430)
                     /locus_tag="VMZ33_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HUR16722.1"
                     /translation="MSRHLGLDLGGTNIKWVVVERLADADGATWTKLADDQVPTLADG
                     GPDNVIQRLAAVGRTAMVEWPDVESVGIGVPGLYDPRTGNTRMLPNLPGDWDGRPMAA
                     VVGKALQRPTFLINDARAFGLAELRLGAGRGVKTMVGITLGTGIGGVIAIDGRVHQGH
                     DGTAGEVGHITIDPNGPDCTCGSNGCLEAFARADRMEELAGAPTAEEAVDRARAGDER
                     ALAALATVGRYLGIGIATMINLVTPDRVVIGGGVAAALDLLRPAIHAELERRVTTTDL
                     AQVDLVAAQLGTWAGGIGAAVHGAEMDGA"
     gene            complement(1441..2448)
                     /locus_tag="VMZ33_05525"
     CDS             complement(1441..2448)
                     /locus_tag="VMZ33_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16723.1"
                     /translation="MSGAVATGPAQAPIHATNSRRSLGSVMRDQPMVPLVGLLVLLVI
                     ITEITRSGTVDPNWAAIIVRTAIPLAILAGCQTLAMLTGGIDLSVAAIASMSGFVVAT
                     LVGGQGLVIAMAVALIAAALAGLITGIGVGVFRVHPLIMTLAMGLVVFGLSNVWQLQN
                     VQTGSGVPPEFRGLGVGLFGGVIPHSLLVFVPVTLLIIVGLRRTGYGRMLFAIGDNPV
                     AARLSGAKSWQILVVLYVISAVLAALAGFIHSGATNTASVTLSNSLLLPSVAAAVIGG
                     TSILGGRGGYGGTILGALILTVVTALLNSLGFKEADRLILFGLIIVLVAAAYTRVTSD
                     A"
     gene            complement(2445..3464)
                     /locus_tag="VMZ33_05530"
     CDS             complement(2445..3464)
                     /locus_tag="VMZ33_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16724.1"
                     /translation="MTTATTTYEEPRVQPMNAVSNFLRRNGWAFGLLALLGAMLVLTR
                     SIQPTYGQAGVQNLAIGALPFAFAAAAQAIIVIGGGIDLSIGSIMALTSVVAAANMKG
                     QSEEYAVVIVIAVLILGFLIGAVNGALIVVTRIPDIIVTLAMSFVWAGSALLVLNVQG
                     GGGAQWLKDIVNGAIGSDPLLEWVPKALIVMAVVVGVIWFIVRRTKLGLWFYSIGSSQ
                     LAAFRSGVPVKRAKLLSYAVGGLFAALAGLSLTAATGIGSPIPGPEHTLLSVAAVVLG
                     GVSLAGGRGGLLGPIVAVYILSLLKTDLTFLRLNSNLATVAQGAILVIVVMVGTLVQI
                     RRARK"
     gene            complement(3461..5050)
                     /locus_tag="VMZ33_05535"
     CDS             complement(3461..5050)
                     /locus_tag="VMZ33_05535"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HUR16725.1"
                     /translation="MALSTETQLLLDATGIAKRYGAVVALKNASLAVLPGEVHALMGA
                     NGAGKSTLVKILTGAVRPDAGRIAIRGKERKVHSPGEARRGGLISVYQEPALIPDLDI
                     SDNLRLTETPVNPFRHWMAELGIPDQSLSAVARKTPLATLRIIDLARALAIEPDVLML
                     DEMTAALPANLTEKVLEVVGRMRGGERSVIFISHRMIEIAAVCDRATVLREGETVGVV
                     DVGPGSEEQIVGLMLGQAVSELQARPSDAAQASDEWRSATPRLSVRGLSAESRLVDAT
                     FDLHQGEVLGVVALEGQGQDELFDILSGSDRPRSGEMLVDGAAVKFGHPADAIRAGVV
                     YVAADRAEALLPQRSVRENIALPFTAPLRKWGPINLSREHRSVAGAVDKLQIDTRAQR
                     EVRRLSGGNQQKVTIARWVAGNVRTMLCFDPTRGIDIRTKSQIYQLLRELAEAGSAVL
                     LYTSELKEIQLACDRAIVIFGGRVVAELDAAEADEAALLRAAYNLTKDAVMPEMIAAE
                     LVAAETGSDAVAAVEKAMTNP"
     gene            complement(5137..6354)
                     /locus_tag="VMZ33_05540"
     CDS             complement(5137..6354)
                     /locus_tag="VMZ33_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUR16726.1"
                     /translation="MTEIKSAKLLGVLGATLLIVAACGGGGATTRPSTAASAAASTAA
                     SAAPSTAASTEPSTAASAEPTQGGTGEYHIGYSNAGGVGNGFREEQVCTAKAEALASG
                     KVSEMTLFHENITGAAGQLTHIRDLIAADVDAIVFNPNDPEGLNEGLQEAEDAGIVTV
                     SVDAYVTHEGTYNLYNNQVMYAELGARWLFEKLGGEGTVWYMRGLAGHPADSDRDIGF
                     KNALADYPNITLVPGDEGVHTGWDPANTTTIATDFVNSGAYDDVDGIWTSGMDSQVVD
                     VIKDAGADFVPIVGADLGAFVAQLLDEENYPGLEGAAVTNTAAVGGAGVNLALKILDG
                     EDIDVAEDAPQPNTVLLTPVVADNVSDEGKATLASWQVDGLDPLWPLGLEIEGYTTYT
                     PEQAVACEGPGGI"
     gene            complement(6410..7201)
                     /locus_tag="VMZ33_05545"
     CDS             complement(6410..7201)
                     /locus_tag="VMZ33_05545"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HUR16727.1"
                     /translation="MAAPPQMRWLAAAQTLLERVATTQAQNIETASEWCADAIASDGL
                     VHLFGTGHSRIPVEEMFPRYGSYPGFNPLVELSMTFHTQIVGANGQRQAMFIERVPGL
                     AEVILGNFEFGPRDVMMVFSASGLTAVPVEMARGARQRGLRVIAVTSVAQSMSTEPDP
                     AAGSRLLDEADLVIDLCTPEADALVDVDDLDTPVGPGSTIAAVAIVNSIKVRVAELLV
                     ERGAMPPVLTRASVVGEERSQALFKKAYREHARRMARALAQQGGD"
     gene            complement(7211..8008)
                     /gene="deoC"
                     /locus_tag="VMZ33_05550"
     CDS             complement(7211..8008)
                     /gene="deoC"
                     /locus_tag="VMZ33_05550"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HUR16728.1"
                     /translation="MTDSADMTPDFAALFDRLTYRDVAKTIDHSLLRPELDDQFIEDN
                     IRLALEYDVASVTMRPTDVEKAAAMVYGSDVKVGTVVGFPHGNHLTSIKVFETDRALS
                     QGALEIDMVIKIGALKSGRDQDVEDDIRAVVEAAHAGGAIVKVIFENAYLTDDEKIRA
                     CRLTEAAGAEFVKTSTGFAPTGATHEDLRLMRANTSAHVQVKAAGGVRTLDALLAVMD
                     IGVTRVGATATKTILDDFKARKSGNPDVMDDSGATAPAAATAGHGAY"
     gene            complement(8016..>8561)
                     /locus_tag="VMZ33_05555"
     CDS             complement(8016..>8561)
                     /locus_tag="VMZ33_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HUR16729.1"
                     /translation="DQNWDEALRVWPDIVRHAQDNGVKITIENCPMIFSHDEWPGGNN
                     IAWSPHIWRRILEQWGGTVGLNYDPSHLVWLMIDQGRFIREFGPHILHFQAKDVLIDR
                     DALYERGTLSGGMGWQIPRLPGLGDANWREIFSNLYHVGYDGDCIIEHEDRRFEATDQ
                     LIKRGFLLAASTLRPYIPKGY"
BASE COUNT         1434 a         2827 c         2839 g         1461 t
ORIGIN      
        1 ctccagcgcg ccgggcagct cgggtgcgac cgttgtcagt cttaggccct ggagcagagg
       61 ctcgagcgtc gcgtcgtcga catcagccgg gatccgaagc tgcgtggagt cgtgggcacc
      121 gcgccgttcc gcggcaagaa acgggccttc caggttgaag ccgagaggct gcgcaccatc
      181 agtcgcgcca ccggccgcca tccagctgcg cacgttctcg gcgaattgac ccagcgcctc
      241 gatcggcgcg gtcactgccg tcggcaggaa cgacgtgacg ccgcggcgtg cgagggcacg
      301 tgccatgccg tccagcgccg cgcgaccgcc catcgcgtcg tggccgcccc agccgtgcac
      361 gtgcacgtcg acgaaacccg gtgcgatgaa gttcgcggtg tcgggcacct cgcgttcctc
      421 aatagcgacg atcaggccgt cgacgacggt cactacgcca ccgacgatgc tgtcgtagag
      481 gacgagctga cccgcgatcc ggtaggcgtt gttcacgcgc cgtccatctc ggcgccatgc
      541 acggccgcgc cgataccgcc cgcccacgtt cccagttgag cggcaacgag gtcgacctgg
      601 gccaggtccg tcgtagtgac acgccgctcg agctccgcgt ggatggcggg ccgcagtagg
      661 tccagtgccg ctgccacgcc accaccgatt acaacccgat cgggcgtgac caggttgatc
      721 attgtggcga tcccgatgcc gaggtaccgc ccgaccgtcg caagtgctgc cagcgctcgt
      781 tcgtcgcccg cgcgcgctcg atcgaccgcc tcttcggcag tcggcgcgcc ggccagctcc
      841 tccatccgat cggcgcgagc gaaggcctcc aggcagccgt tgctgccgca ggtgcagtcg
      901 ggtccgttgg gatcgatcgt gatgtggccg acctcgccgg ccgtgccgtc atggccctgg
      961 tgcacgcgac cgtcaatcgc gatcacgccg ccaatgccgg tgcccaaagt tattccgacc
     1021 atcgtcttga ctccgcgtcc ggcgccgagg cgcagctcgg ccaggccaaa ggcgcgcgcg
     1081 tcgttgatca ggaatgtcgg gcgctgcagg gctttgccga caaccgctgc catcggccgc
     1141 ccatcccaat cgccggggag attgggcagc atgcgtgtgt tgccggtgcg tgggtcgtac
     1201 aggccgggca ccccgatgcc gaccgattcg acgtccggcc actcaaccat ggctgtgcgg
     1261 cctaccgccg caaggcgctg gattacgttg tcagggcccc cgtcggcaag agtcggcacc
     1321 tgatcatcgg cgagcttggt ccaggtcgcg ccgtcggcat ctgccaggcg ctcgaccaca
     1381 acccatttga tgttcgtgcc gcccagatcg agcccaagat gccgactcac ggcgctggcg
     1441 tcaggcgtca gatgtcacgc gtgtgtaggc ggcagccacg aggacgatga tcaggccgaa
     1501 gaggatcagg cggtcggctt ccttgaagcc gagcgaattg agcaatgccg taaccacggt
     1561 caggatcagg gcgcccagga tcgtcccgcc gtaaccaccg cgtccgccca ggatcgacgt
     1621 accgccgatg accgctgcgg cgacggacgg gagcagaagc gagttggaga gcgtgacgct
     1681 ggcggtgttc gtcgcaccac tgtggatgaa tcctgcgagc gcagcaagca cggctgagat
     1741 cacatacaga acgacgagga tctgccacga cttggcgcca gagaggcgcg ccgcgacggg
     1801 gttatcaccg atggcaaaca gcatgcggcc gtagcccgtg cgcctgagcc cgacgatgat
     1861 caacagggtc accggcacga acaccagcag gctgtgcggg attaccccgc cgaacaggcc
     1921 cacgccgagc ccgcgaaatt cgggcgggac acccgagccg gtttgcacgt tctggagctg
     1981 ccagacgttt gataagccaa agacgaccag gcccattgcc agggtcatga tcagtgggtg
     2041 cacgcggaac acgccgacgc cgatcccggt gatgagtccg gcgagcgcag ccgcgatcaa
     2101 cgcaacagcc atggcgatga cgagtccctg cccgccgacg agagtcgcca cgacaaagcc
     2161 cgacatcgag gcgatggccg ccaccgacag gtcgatgccg ccggtgagca tggccagcgt
     2221 ctggcatccg gcgaggatcg cgagcgggat agcggtgcga acgatgatgg ccgcccagtt
     2281 gggatcgacc gtgcccgagc gcgtgatctc cgtgatgatg accagcagca cgagcagtcc
     2341 gacgagcggc accatcggct ggtcgcgcat gacggagccg aggcttcggc gcgagttggt
     2401 cgcgtggatg ggcgcctgcg cgggacctgt ggccactgcg ccgctcactt ccttgccctc
     2461 cggatttgca ccaacgtacc gaccatgacc acgatcacca ggatcgcgcc ctgcgcgaca
     2521 gtggccaggt tcgagttgag cctcaggaaa gtcaggtccg tcttcagcag tgagaggatg
     2581 tagaccgcga cgatggggcc caggaggccg cctctaccgc cggccaggct gacgcctccg
     2641 agcaccacgg cggcgacgct gagcagcgtg tgctcagggc cggggatcgg cgaaccgatg
     2701 cccgttgccg cggtcaggct gagaccggcg agcgccgcga agaggccgcc gactgcgtag
     2761 gacaggagct tggcgcgctt gacggggacg ccgctgcgga aagctgccag ctggctgcta
     2821 ccgatggagt agaaccacag tccgagcttg gttcggcgga cgatgaacca gatgacgccg
     2881 acgaccacgg ccatgacgat cagggctttg ggcacccact cgagcagggg gtcgctgccg
     2941 atcgctccgt tgacgatgtc cttgagccat tgcgcgccgc cgccgccctg gacgttgagc
     3001 accagcaggg cggaaccagc ccagacgaac gacatggcca gagtcacgat gatgtcgggg
     3061 atgcgcgtca caacgatcag tgcgccgttc acggcgccga tgaggaagcc caggatcagc
     3121 accgcgatga cgatgacgac cgcatattcc tcggactgac ccttcatgtt ggccgcggca
     3181 acgacgctcg tcagggccat gatcgagccg atcgacaggt cgattccgcc gccgatgacg
     3241 atgatcgcct gcgcggcggc cgcaaaggcg aacggcaggg ccccgatggc gaggttctgc
     3301 acgccggcct ggccatacgt cggctggatg ctgcgggtca gcacgagcat cgcgccgagg
     3361 agcgccagaa ggccgaacgc ccagccgttg cgccggagga agttcgatac cgcgttcatg
     3421 ggttgcacgc gcggctcttc gtacgtggtt gtggcggttg tcatgggttg gtcatcgcct
     3481 tttcgacggc ggcgaccgcg tcgctgccgg tctccgcggc gacgagctcg gctgcgatca
     3541 tctcgggcat gacggcatcc ttggtgaggt tgtaggccgc gcgcaggagg gctgcctcat
     3601 cggcctcggc cgcgtccagc tcggcgacga ctcggccacc gaagatcacg atcgcgcggt
     3661 cgcacgcgag ctggatctcc ttgagctcag acgtgtagag gagtacggcc gacccggcct
     3721 ctgcaagctc gcgcagcaac tggtagatct ggctcttggt gcggatgtcg atgccgcgcg
     3781 tggggtcaaa gcagagcatg gtgcgcacgt tgcccgctac ccaacgcgcg atggtgacct
     3841 tctgctggtt gccaccggat aggcgacgga cctcacgctg agcgcgggtg tcgatctgca
     3901 gcttgtcgac ggcgccggcg accgacctgt gttcgcggga gaggttgatc ggcccccact
     3961 tccggagcgg cgccgtgaat ggcagggcga tgttctcgcg aaccgatcgc tgcggcaaca
     4021 gggcttcggc gcggtcggcg gcgacataaa cgacgccggc cctgatggcg tccgccggat
     4081 ggccgaattt caccgcggcg ccatcgacca gcatctcgcc gctcctgggt cggtccgagc
     4141 cggaaagaat gtcgaacagc tcgtcctgtc cctggccttc gagcgccacc acgccgagta
     4201 cctcgccctg atggaggtcg aaggtggcat ccacaaggcg cgactcagca ctgaggccgc
     4261 gaaccgagag ccttggagta gccgaacgcc actcgtcgct ggcctgcgcc gcgtcgctcg
     4321 ggcgggcctg cagctcagac accgcctggc cgagcatcag gccgacgatt tgctcctccg
     4381 agcctggtcc aacgtccaca acgccgactg tctcaccctc gcgcaggacc gtagcgcgat
     4441 cgcagactgc cgcaatctcg atcatgcggt gtgagatgaa gatcaccgac cgttctccgc
     4501 cgcgcatgcg acccacgacc tcgagcacct tctcggtgag gttcgcgggg agcgcggcag
     4561 tcatctcatc gagcatcagg acgtcgggct cgatggccag agcgcgcgcg aggtcgatga
     4621 tgcgcagcgt ggccagcggc gtcttgcggg ctacggcgga tagcgactga tcggggatgc
     4681 ccagctcagc catccagtgc cggaacgggt tgaccggcgt ctccgtaagg cgcaggttgt
     4741 cgctgatatc gaggtccgga atcagcgcgg gttcctggta gaccgatatg aggccgccgc
     4801 ggcgagcctc gcccggggag tgcaccttgc gctccttgcc gcggatcgcg atgcggcccg
     4861 cgtccgggcg cacggcgccc gtgaggatct tgacaagcgt gctcttgccg gcgccattag
     4921 cgcccatcag agcgtggact tcgcccggca gcactgccag tgaagcgttc ttgagtgcga
     4981 cgacagcccc ataccgcttc gcgatgcccg tcgcatccag gagcagctgt gtctcagtgc
     5041 tgagcgccat gcgttccctt gaaacgtggg atgcaacgag cgggaggccg gcgaaccgac
     5101 ctcccgctca tggtgactcg actacgcgag cggaacctag atcccgcccg ggccttcgca
     5161 ggcgacagcc tgctccggcg tgtacgtcgt gtagccctcg atctcgaggc ccaggggcca
     5221 cagaggatcg agcccgtcga cctgccacga cgcaagcgtc gcctttcctt cgtcgctgac
     5281 gttgtcagcc acgaccgggg tcaggagcac ggtattgggc tgcggagcat cctcagccac
     5341 gtcaatatcc tcaccgtcga ggatcttcag cgcgaggttg acacctgcgc caccgacagc
     5401 cgcggtgttg gtcaccgctg cgccctcgag gcccggatag ttttcctcgt cgaggagctg
     5461 cgcaacgaac gcgccgaggt cggcgccgac gatcggcacg aagtccgcgc cggcgtcctt
     5521 gatcacgtcg acaacctgtg agtccatgcc tgacgtccag atgccatcta cgtcgtcata
     5581 agcaccgctg ttgacgaagt cggtcgcaat cgtggtcgtg ttggccggat cccagcctgt
     5641 gtggacaccc tcgtcacccg ggacgagcgt gatgttcggg tagtcggcca gagcgttctt
     5701 gaacccgatg tcgcggtcgc tgtctgccgg atgacccgcc agaccgcgca tgtaccaaac
     5761 ggttccctcg ccaccgagct tctcgaacag ccagcgtgcg ccgagctccg cgtacatgac
     5821 ctggttgttg tacaggttgt acgtgccctc atgcgtcacg taggcgtcga ccgacaccgt
     5881 gacgatgccg gcatcctcgg cctcctggag accttcgttc aggccctcag gatcgttcgg
     5941 gttgaagacg atcgcgtcga cgtcagccgc gatcaggtcg cggatgtgcg tcagctgacc
     6001 ggccgcgccc gtgatgttct cgtggaagag cgtcatctcc gagaccttgc ctgaagcgag
     6061 tgcctcagcc ttggccgtgc agacctgctc ctcacggaag ccgttaccga cgccaccggc
     6121 gttcgagtaa ccgatgtggt actcgccggt gccgccctgt gtgggctcag ccgacgctgc
     6181 cgtgctgggt tcggttgatg ccgcagtgct cggtgcagct gatgctgccg tgcttgctgc
     6241 ggcggatgcc gctgtcgacg gcctggtcgt cgcgccaccg ccgccgcatg ctgcgacgat
     6301 caggagcgtg gcgcctagga cgcccaagag cttcgcgctc ttaatttcag tcatctgtgt
     6361 gagttcctcc tcaggatttg cgtaaacatg tacctcgtcg tgcctcgcgt cagtcacctc
     6421 cttgctgtgc gagagcgcgc gccatacgtc tggcgtgttc tcggtatgcc tttttgaaca
     6481 gggcctgtga ccgctcttcg cccaccacgg acgcccgggt gagaactggc ggcatcgccc
     6541 cgcgttcaac aagcagctcc gcgacgcgaa cctttatcga gttcacaatc gcaacggccg
     6601 cgatggtaga ccctggcccc accggagtgt cgagatcgtc gacgtcgacg agcgcgtctg
     6661 cctcaggcgt gcacaggtcg atgacgaggt cggcctcgtc cagcagtcgg ctgcctgccg
     6721 ctggatcggg ctcggttgac atcgattggg cgaccgaggt gaccgcgatc accctcaggc
     6781 cacgctgtcg ggcgccccgg gccatctcca ccggcacggc cgtcaggccg ctcgccgaga
     6841 acaccatcat cacgtcccgg gggccgaatt cgaagttgcc aaggatcacc tcggccaggc
     6901 cgggcacgcg ctcgatgaac atcgcctggc gctggccgtt ggcgccgacg atctgcgtgt
     6961 ggaacgtcat cgacaactcg accagcgggt tgaacccggg atacgagccg tagcgcggaa
     7021 acatctcctc gaccgggatg cgtgagtggc ccgttccgaa caggtgcacc aggccatcgc
     7081 tggcgatcgc gtcggcgcac cactcgctgg cggtctcgat gttctgcgcc tgcgtcgtgg
     7141 cgacgcgctc gagcagggtc tgggcggccg cgagccaacg catctgcgga ggtgccgcca
     7201 cggctgtcat ctagtaggcg ccgtgtccgg cagtcgctgc ggccggtgcg gtcgcgccgc
     7261 tgtcgtccat cacgtccggg ttcccggact tgcgcgcctt gaagtcgtcg aggatcgtct
     7321 tggtcgccgt tgcgccgacg cgcgtgacgc cgatgtccat cacggcgagc agcgcgtcga
     7381 gcgtccgcac gccgccggcg gcctttacct gcacgtgcgc tgaggtgttg gcgcgcatca
     7441 agcgcaggtc ctcgtgcgtt gcgccggtcg gcgcgaagcc ggtcgacgtc ttgacgaact
     7501 cggcgcccgc agcttccgtg agccggcacg cgcgaatctt ctcgtcgtcg gtcaggtacg
     7561 cgttctcgaa gatcaccttg acgatggctc cacccgcgtg cgcagcctca accacggccc
     7621 ggatgtcatc ttcgacgtcc tgatcgcgtc ccgacttcag cgcgccgatc ttgatgacca
     7681 tgtctatctc gagggcgccc tgcgaaaggg ctcggtcggt ctcgaacacc ttgatcgagg
     7741 tcaggtggtt gccgtgcgga aagccgacga ccgtgccgac cttcacgtcc gagccgtaga
     7801 ccatggccgc agccttctca acgtcggtcg gacgcatggt caccgaagcg acgtcgtatt
     7861 ccagcgcgag tcggatgttg tcctcgatga actggtcatc gagctccggg cgcagcagcg
     7921 agtggtcaat cgtcttggcg acatcgcggt acgtcaggcg gtcgaaaagt gccgcgaaat
     7981 cgggggtcat atcggcggag tcggtcatgc gttccttagt agcccttggg gatgtatggc
     8041 cgcagcgtgc tggccgccag caggaagccg cgcttgatca gctggtcggt cgcttcgaag
     8101 cgacgatctt catgttcgat gatgcagtct ccgtcgtacc ccacgtgata aaggttggag
     8161 aagatttctc gccaattcgc atcacccaga ccaggcaggc gtggaatctg ccagcccatg
     8221 ccgcccgaga gggtcccgcg ttcgtagagg gcgtcacggt cgatgagcac gtccttggcc
     8281 tggaagtgga ggatgtgcgg gccgaactcc cggatgaatc gaccctgatc gatcatcagc
     8341 cagacgaggt gcgacgggtc gtagttgagc ccgacggtgc caccccactg ctcgaggatg
     8401 cgacgccaga tgtgcggcga ccaggcgatg ttgttgccgc ccggccactc gtcgtggctg
     8461 aagatcatcg ggcagttttc gatggtgatc ttgacgccgt tgtcctgggc gtggcggacg
     8521 atatccggcc atacgcgcag cgcctcatcc cagttctggt c
//
LOCUS       DATLCO010000233         2082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_382156, whole genome shotgun sequence.
ACCESSION   DATLCO010000233 DATLCO010000000
VERSION     DATLCO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382156"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VMZ33_05560"
     CDS             <1..237
                     /locus_tag="VMZ33_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HUR16730.1"
                     /translation="FWDAGAYEPGRGQASFDKQFVRDYLETLAWDKTYPGPELPPDVV
                     AGTRARYAEAFERITGGSFDSYLLDDRVHGEATS"
     gene            237..518
                     /gene="purS"
                     /locus_tag="VMZ33_05565"
     CDS             237..518
                     /gene="purS"
                     /locus_tag="VMZ33_05565"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HUR16731.1"
                     /translation="MAAFRFAVDVMPRDGILDPQGRAVEAALPRLGATSVHEVRVGRR
                     IELTVEAESEGEASEVVHRLAGEFLANPLIESWVIESLASTPAGVGVPA"
     gene            515..1201
                     /gene="purQ"
                     /locus_tag="VMZ33_05570"
     CDS             515..1201
                     /gene="purQ"
                     /locus_tag="VMZ33_05570"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HUR16732.1"
                     /translation="MKVGVVVFPGSNCDRDALHAARLAGADAELLWHEDSELHGAGAV
                     ILPGGFAHGDYLRAGAIARVSPIMGAIRDHAARGGLVLGICNGFQVLTEAGLLPGALR
                     RNDTLRFEHRWVRLSVARHDTPFTWAVPHGMTLRLPIAHGEGSYYLATDELDALEANG
                     QVLFRYALPEGNPNGSLRDIAGVTDANGRVAGLMPHPERASEALLGSDDGLLLMRSLV
                     ESAARAEAAA"
     gene            1207..>2082
                     /locus_tag="VMZ33_05575"
     CDS             1207..>2082
                     /locus_tag="VMZ33_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HUR16733.1"
                     /translation="MLDRPAIEPANELHRQIGLTDEELDAIRVRLGRDPNDVELAMFS
                     VMWSEHCSYKSSRSLLRTLPTDGPDVLAGPGENAGVVRIGDGLAVAFKLESHNHPSAV
                     EPYQGAATGVGGIIRDVTAMGARPIALLDALKFGWPDDERTRRLVRGVVRGVGGYGNA
                     VGVPTVGGELTFDSTYTDNPLVNVMCLGLVAENGLARAIARTPGYLAVLYGSPTGRDG
                     IGGASVLASANFDEKSAHMRPSVQIGDPFAGKLLIEASLELVERGLVDGLQDLGAAGL
                     TCATSEMADRGGTGIR"
BASE COUNT          310 a          690 c          741 g          341 t
ORIGIN      
        1 ttctgggacg cgggcgcgta cgagccgggt cgtgggcagg cgagcttcga taagcaattc
       61 gtccgcgact acctcgagac gctcgcctgg gacaagacct atcccggccc agagctgcct
      121 cccgacgtgg tcgccggcac gcgcgcccgc tatgccgagg cgttcgagcg gatcaccggc
      181 gggtcgttcg acagctacct gctcgacgac cgcgtccacg gtgaggcgac gtcctgatgg
      241 cggcgttccg cttcgcagtc gacgtgatgc cgcgtgacgg gatcctggat ccgcagggcc
      301 gagccgtcga ggcggcgctg ccgcgactcg gggcgacctc agtccacgaa gtgcgtgtgg
      361 ggcgtcgcat cgagctgacg gttgaggcgg agtccgaggg cgaggcgagc gaggttgtcc
      421 atcggctggc gggggagttc ctggccaatc cgttgatcga gagctgggtc attgaatcgc
      481 tggcatccac tccagctggg gtgggggtcc ctgcttgaaa gtcggagtcg tcgtctttcc
      541 cggctccaac tgcgatcggg acgcgctcca tgcggcgcgt ctggccggcg ccgatgccga
      601 gctcctgtgg cacgaagact ccgagctcca cggggctggt gcggtgatcc ttccgggtgg
      661 cttcgcccat ggcgactacc tgcgcgcggg tgcgatcgcg cgggtcagcc cgatcatggg
      721 cgcgattcgc gaccacgccg cgcggggcgg cctggtcctg ggcatctgca atggcttcca
      781 ggtgctcacg gaagcggggc ttctccccgg ggcgctgcga cggaacgaca cgctccgctt
      841 cgagcatcgt tgggtgcgcc tctccgtagc gcgccacgac acgccgttca catgggctgt
      901 gccgcacggc atgacactgc ggctgccaat cgcccatggc gagggcagct actacctggc
      961 aaccgatgag ctggacgcat tggaggcaaa cggccaggtg ctgttccgct acgccctgcc
     1021 cgaaggcaat cccaacggat cgctgcgcga cattgccggc gtcacggatg ccaacggccg
     1081 cgttgcgggg ctgatgccgc accccgagcg agcctcggaa gcgttgctcg gctccgacga
     1141 cgggctgctg ctgatgcgct ccctcgtcga gagcgcagcg cgggcggagg ctgcggcttg
     1201 accgacgtgt tggaccgtcc ggcgatcgaa ccggccaacg aactgcaccg ccagatcgga
     1261 ctgaccgatg aggagctcga cgccatccgg gtacgacttg ggcgcgaccc caacgacgtc
     1321 gagttggcca tgttcagcgt gatgtggtcg gagcactgct cgtacaagag ctcgcgatcc
     1381 ctgctgcgaa cactgccgac cgacgggccg gatgtgctgg cgggcccggg cgagaatgcc
     1441 ggcgtcgtcc ggatcggcga tgggcttgcc gtcgcgttca agctcgaatc gcacaatcat
     1501 cccagcgccg ttgagcccta tcagggggcc gctaccggag tgggcgggat catccgtgac
     1561 gtcaccgcca tgggcgcgcg gccgattgcg ctgctcgacg ccctgaagtt cggatggccc
     1621 gacgacgaac gaacacgccg gctcgtacgc ggcgtcgtcc gtggcgtggg cggctacggc
     1681 aacgcggtgg gtgtgccgac cgttggcggc gagctgacgt tcgactcgac ctataccgac
     1741 aacccgctgg tcaacgtgat gtgcctcggc ttggtcgcgg agaacggact cgcccgcgcc
     1801 atcgctcgca cgcccggata cctggcggtc ctgtacggct cgccgacggg gcgcgatggg
     1861 atcggcggcg cgtccgttct cgccagcgcc aacttcgacg agaagtcggc ccacatgcgc
     1921 cccagcgtcc agatcgggga tccgttcgca ggcaagctgc tcatcgaggc ctcgctcgag
     1981 ctcgttgagc gtgggctggt tgacggcctc caggatctcg gcgccgctgg gctgacgtgc
     2041 gccaccagcg agatggccga tcgcggcggc accggcatcc gc
//
LOCUS       DATLCO010000234         4043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8388374, whole genome shotgun sequence.
ACCESSION   DATLCO010000234 DATLCO010000000
VERSION     DATLCO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8388374"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1074)
                     /locus_tag="VMZ33_05580"
     CDS             complement(154..1074)
                     /locus_tag="VMZ33_05580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16734.1"
                     /translation="MTLPTAPVTTPEVLAPRRSARRRFGTWMHSRPRLRLGFLLAGPV
                     GWLLIAYLGSLFILLLNALWAKDSFTGTVQPFNWSLDAFQTILDGEAYRTVAVRTVGM
                     AILVTITDALLALPIAYYMARIASPRTRSLMVVAILIPLWAAYLVKVYAWRIILQGNG
                     LVDWVLAPFGLSGPGLSEDLNAWLVLSYLWLPYMILPIYAGFERIPGSLLEASADLGA
                     RSLTTFRRVVLPLVFPALVAGSIFSFSLTLGDYITPDLVADAKFIGNVIYDNAANVNL
                     PLAAAYSLIPIAIMIGYLFVARRLGAFESL"
     gene            complement(1154..2446)
                     /locus_tag="VMZ33_05585"
     CDS             complement(1154..2446)
                     /locus_tag="VMZ33_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR16735.1"
                     /translation="MVIAACGGSGATTRPSTGASNAASAGPSAASSAGQSEAPATDSP
                     TAEPTDVAATDEPSSEPTIGPTTEPSTSAGIGPGEGELNLVIWTGYAERGASDPVYDW
                     VTPFEEETGCMVNTTDMADSNNGVSLMQSGSYDGISASGDATTRLIQGGLAAPVDTSV
                     FTNYDAVFDSLKDLPHNTVDGVNYGVPHGRGANLLAYNTETFSEAPTSWDPIWEGGSD
                     YAGKISIYDSSIFIADAALHLQSTQPELGITDIYQLNEEQFNAAVALLEELKANNPLY
                     WGLAADQIASYGAGEVVVGTTWQYQVNVLQADAQPIEGVLPDEGSTGWSDTWMMSAEA
                     AHPNCMLLWMDWMMSPEASALATIWFGEAPTSQAACDFAETVSPGHCGAFHATDEEYF
                     SKVHYWATPQEDCADEDPATTCKTQDDWVEAWTDLRGS"
     gene            complement(2503..3558)
                     /locus_tag="VMZ33_05590"
     CDS             complement(2503..3558)
                     /locus_tag="VMZ33_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16736.1"
                     /translation="MTDAPRSAVTLENIEKRFGDVLAVNGVNLEVRDGEFFSMLGPSG
                     SGKTTTLRMIAGFEIPTAGRVLLHGRDVTNDPPFERDVNTVFQDYALFPHMTVGDNVA
                     YGLVIKKVPREDRQRRVKEALAMVRLDGYDKRRPGQLSGGQRQRVALARALVNRPRVL
                     LLDEPLGALDLRLREEMQIELKQIQQEVGITFIYVTHDQGEALTMSDRMAVFNQGRIE
                     QVGSPADVYEKPTTTFVAGFVGTSNLLTGDAARSVIGEQGTFTVRPEKIRVADADADA
                     DATADDSDCSALGHIKKVVYLGPDTRFYVELDAGPTLVVEQQNLSTSSSDALAQEGKR
                     VRLMWKRVHNLELAGAN"
     gene            complement(3746..>4043)
                     /locus_tag="VMZ33_05595"
     CDS             complement(3746..>4043)
                     /locus_tag="VMZ33_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16737.1"
                     /translation="VITYPSLYEGFGNALIESVFFRKLTVVNRYSVFDADIRPLGFRF
                     IELNGAVTDEAVDQLRTALEDRSAARLDADLNFRLAAENFGYDRLRRELRALLA"
BASE COUNT          718 a         1268 c         1359 g          698 t
ORIGIN      
        1 caaaccagtg cagcgtgaaa ccgaccggtg gccaggattg gatgcggctc tcgttgaacg
       61 cgtagatcgc cagcacgacg atcggcagat agatgaacgc cagcaccagc agcgacacga
      121 cgcgcagcgc gatgcgtacc aaacgtggct ctgtcacagc gattcgaatg cgccgaggcg
      181 cctggcgacg aacaggtagc cgatcatgat cgcgatgggg atcaaggagt aggccgccgc
      241 gagtggcagg ttcacgttgg cggcgttgtc gtagatcacg ttgccgatga acttcgcgtc
      301 ggccaccagg tccggcgtga tgtagtcacc gagcgtgagg ctgaacgaga aaatcgagcc
      361 cgcgacaagc gccgggaaca ccagtggcag gacgaccctg cggaaggtcg tgaggcttcg
      421 ggcgccgagg tcggcggagg cctccagcag cgagcccggg atgcgctcga agccggcgta
      481 tatcggcagg atcatgtagg gcagccacag gtagctgagg acgagccacg cgttgaggtc
      541 ttcggataga ccaggacccg acaggccgaa cggcgccagg acccagtcga cgagcccgtt
      601 gccctggagg atgatccgcc acgcgtaaac cttgacgagg tacgcggccc aaagcggaat
      661 gaggatggcg acgaccatca gactgcgcgt gcgcggcgac gctatgcgcg ccatgtaata
      721 ggcgatcggg agcgccagta gcgcgtcggt gatggtgacc aggattgcca tccccaccgt
      781 gcgcactgcg accgttcggt aggcctcgcc atcgaggatg gtctggaagg catcgagcga
      841 ccagttgaac ggttggaccg tgcctgtgaa cgagtccttc gcccagagag cgttcagcag
      901 caggatgaag agcgacccca ggtacgcgat cagtagccag ccaaccggtc cggcgagcag
      961 gaaccccagc cgcaaacgcg gacgcgagtg catccacgtg ccgaaacggc gacgagcgct
     1021 gcgccgtggc gcaagcacct cgggcgtagt gacgggagcc gtaggcaggg tcatcgaaag
     1081 aaggcgctat gaaacaaggg ctccccggct tgcgccgggg agccccattg atcgactggc
     1141 tgggttgctc ggctcagctg ccgcgcagat cggtccaggc ttcgacccag tcgtcctggg
     1201 tcttgcaggt cgtggccgga tcttcgtccg cgcagtcctc ctgcggcgtc gcccagtaat
     1261 gaaccttcga gaaatactcc tcgtcagtcg catggaaggc gccgcaatgc cccggcgaga
     1321 cggtctcggc aaagtcgcag gcggcctggc tggtcggcgc ctcgccaaac cagatggtcg
     1381 cgagagcgct cgcctcaggc gacatcatcc agtccatcca gagcaacatg cagttcgggt
     1441 gtgcagcctc ggcggacatc atccaggtgt cagaccagcc cgtggagccc tcgtcgggaa
     1501 ggaccccttc gattggctgc gcgtctgcct gtaggacatt gacctggtac tgccatgtgg
     1561 ttccgacgac gacttcgccg gcgccgtaag acgcgatctg gtcagccgcg aggccccagt
     1621 agagcgggtt gttcgccttg agctcctcaa ggagcgcgac ggcggcgttg aactgctcct
     1681 cgttcagctg gtaaatgtcc gtgatgccca gctctggctg ggtgctctgg aggtgcaggg
     1741 cagcgtcggc gatgaaaatc gaagagtcgt agatgctgat cttgccggcg tagtcggagc
     1801 caccttccca gatcggatcc cagctcgtgg gtgcctcact gaatgtctcc gtgttgtagg
     1861 ccagaaggtt cgcgccgcgc ccgtgcggca cgccgtagtt gacaccgtcc acggtgttgt
     1921 gcgggaggtc cttgaggcta tcgaaaacgg cgtcgtagtt ggtgaagacg ctcgtgtcga
     1981 ccggcgccgc caggccgccc tgaatcagac gtgtcgttgc gtcacctgat gccgagatgc
     2041 cgtcatagct gcccgactgc atcagcgaga cgccgttgtt cgagtcggcc atgtcagtcg
     2101 tgttgaccat gcagcccgtc tcttcctcga acggtgtgac ccagtcataa acagggtcgc
     2161 ttgcgccgcg ctcggcgtag cccgtccaga tcacgaggtt cagctcaccc tcgccgggcc
     2221 cgatgcccgc cgacgttgaa ggctcggtgg tcggcccgat ggtcggctcg ctgctcggct
     2281 catcggtggc agcgacatca gtcggctccg cggtcggcga atcggtggcc ggcgcctcgg
     2341 actgcccggc gctcgacgcc gcgcttggtc cggcgcttgc agcgttactc gctccagtcg
     2401 acggtcgagt cgtcgctcca ctgccgccgc aggcagcgat gaccatggcg acgatcgcaa
     2461 gaacccccca ggtgcgtgac gtacgcaaaa cgaaccctcc tgttagttgg cccccgctaa
     2521 ctccagatta tgaacacgct tccacatcaa gcgcaccctc ttgccttcct gcgcgagagc
     2581 gtcgctcgac gacgtggaca aattctgttg ctcgacgacg agcgttggac ccgcatcgag
     2641 ctccacgtag aagcgcgtgt cgggccccag gtagacgacc ttcttgatat gaccgagagc
     2701 gctgcagtcg ctgtcgtccg cagtcgcgtc ggcgtcggcg tcggcgtcgg cgacacggat
     2761 cttctccggg cggacggtga acgtgccctg ctcgccgatc acgctgcgtg ccgcatcgcc
     2821 cgtgagcagg ttgctcgttc cgacgaaccc agcgacgaag gtcgtcgtcg gtttctcgta
     2881 gacgtcggcg gggctgccca cctgctcgat gcggccctgg ttgaagacag ccatccggtc
     2941 cgacatcgtc agcgcctcgc cctggtcgtg cgtcacgtag atgaacgtga tgccgacctc
     3001 ctgctggatc tgcttcagct cgatctgcat ctcttcgcgg agccgtaggt cgagggcgcc
     3061 gagtggctcg tccaggagca ggacccgggg tcgatttacc agcgcgcggg cgagggcgac
     3121 gcgctgccgt tggccaccac tgagctggcc tgggcggcgc ttgtcgtagc cgtcgaggcg
     3181 gaccatcgcg agcgcttcct tgacgcgcct ctggcggtcc tcacgtggaa ccttcttgat
     3241 gacaagccca tacgcgacgt tgtcgccgac cgtcatgtgc ggaaacagcg cgtagtcctg
     3301 gaaaacggtg ttgacgtcac gctcgaacgg cgggtcgttg gtcacgtcgc ggccatggag
     3361 cagcacgcgg ccagcggtcg ggatctcaaa cccggcgatc atgcgcagcg tcgtggtctt
     3421 gcccgagccg gacgggccga gcatcgagaa gaactcgccg tcgcggacct ccagattcac
     3481 gccgttgacg gcaaggacgt cgccgaagcg cttttcgatg ttctcgagcg tgacagcgct
     3541 tcgtggcgcg tcggtcaagg acccctccgc tttgcggcgc accccccgca tgacgttccg
     3601 cggagtgtag cgatccccgc gcggctggca ggggccacgg cgcggattcc tgagtcgctc
     3661 agttggataa tttttgccgc cgaggcgagg gcggccgggg tcgatatcgg ccggcgggtt
     3721 cagcgtcgag cgtcagcgag gacggtcagg caagcagggc gcgcaactcg cggcgcaggc
     3781 ggtcgtagcc gaagttctca gccgcgagcc tgaaattgag gtcggcatcg agtcgggcgg
     3841 ctgatcggtc ctccagggca gtacgcagct gatcgaccgc ctcgtcggtc accgccccgt
     3901 tcagctcgat gaagcggaag ccgagcggcc ggatgtccgc atcgaagacg gagtagcggt
     3961 tgacgacggt cagcttccgg aagaagacgg actcgatcag cgcgttgccg aagccctcgt
     4021 acaggctcgg gtacgtgatg aca
//
LOCUS       DATLCO010000235         4581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6900959, whole genome shotgun sequence.
ACCESSION   DATLCO010000235 DATLCO010000000
VERSION     DATLCO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6900959"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1150
                     /locus_tag="VMZ33_05600"
     CDS             146..1150
                     /locus_tag="VMZ33_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HUR16738.1"
                     /translation="MLEHLSVMLIAVIALAIAFDYINGFHDTANAIATSVATRALDPR
                     AAIAMATAFNFIGAFAGTAVAKTIGSGLVDESTSTQGVIAAALIGAITWNLLTWYYGL
                     PSSSSHALIGGLFGATLVSAGPGAFKLDGIVQKVIFPMIASPVVGFFVALALVVLLFW
                     LFFRAQRRPMTTVFRRLQIVSAGFMAFSHGSNDAQKTMGIITLGLFSAGALSTNDVPW
                     WVIVVAATAISLGTAVGGWRIMHTMGHRVVKLEPVHGFAAETTAATVIFTAAHFGMPV
                     STTHVISSAIIGVGSARGLRRVRWGVARSILLAWIITIPAAALVAAGSWFVLHLVGLG
                     "
     gene            1162..1779
                     /locus_tag="VMZ33_05605"
     CDS             1162..1779
                     /locus_tag="VMZ33_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HUR16739.1"
                     /translation="MPLSLIPKDTKFLDMFATSGENLAAATRELVEFVDKFDNLDERI
                     ADIQRLEKRGDQIDREINRRLEAAFVAPFDREDIHELTVRLDDVVDYVQAVGESLIIY
                     DVKAPTDECRRLASILADQGQELSAALTKLDKMKDLSVHLERIHDLEHEADGVSRKAI
                     GRLFRENHDPLDVIKWREIYLELENAIDAAEDAAEAIERMIHKRV"
     gene            1843..2940
                     /gene="ruvB"
                     /locus_tag="VMZ33_05610"
     CDS             1843..2940
                     /gene="ruvB"
                     /locus_tag="VMZ33_05610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HUR16740.1"
                     /translation="MQEPDRSVDGDALTLEEVAVETSLRPRLLAEYIGQSAVKQNLEI
                     LLSAARQRNESADHVLLYGPPGLGKTTLANIIARELGVNLHATSGPAVERPGDLAAIL
                     TNLEERDVLFIDEIHRLNHSVEEILYPAMEDFAIDVMIGRGPSARSLRLGLKPFTVIG
                     ATTRAGRISGPLRDRFGAVYRLDYYSGEDLATIVRRSAGILAVDIDDAAVNVIARRGR
                     GTPRVVNRLLRRVRDYAQVRSDGRIGPTEAADAMNAMDIDDEGLDTTDRRLLAAIVQK
                     FASGPVGGAAVAAVIGEEVDTIEDVYEPYLLQLGFLDRTPQGRIATERARDHLRRLGY
                     EVPTPRRAERSDSGLWDGINVEGRTESETVA"
     gene            2937..3980
                     /locus_tag="VMZ33_05615"
     CDS             2937..3980
                     /locus_tag="VMZ33_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR16741.1"
                     /translation="MTDAVMESSAPTEHAVVTRGLMKSYGRRVALTGLDMSVPRGQIY
                     GFLGPNGSGKTTALRLLTGLLRPNGGTIELFGQPYSWTDRKRLFRVGSLIETPSFYPY
                     MSGRENLLVLGASGPPTPAARIDQVLEYVGMSGRAKDAVQTYSLGMKQRLGIAAAMLS
                     DPDLLLLDEPANGLDPGGMVAMRETLRWFTRELGKTVVISSHLLHEVEQLADVVGIID
                     SGRLIREGNLQKLMEDAGRVKVRVAPSEMAQAGSILRSFAPDRPLYGVDSGDGAGWFT
                     VNVSPARAAEINKVLAEAGIYASGLEPGTDLETVFLEITGSISGHVNAPPTMPGQPPA
                     PPAPMPPPVPPGA"
     gene            3977..>4581
                     /locus_tag="VMZ33_05620"
     CDS             3977..>4581
                     /locus_tag="VMZ33_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16742.1"
                     /translation="MRLVKAELLKARRRSATWIVLVVTLVLMGVLYLLSGPVFKGLGV
                     IEFPQAYVVIGQFAYGLGGLLALIYAAAFVGADWNWGVIRNVVARGESREAYLLAKAA
                     ALAILLGIALLILFLFGIVMSYVTGFAWDVPVASPLRAEGLSDLLTWIGLGFPVLLQR
                     AAIGFAVAVILRSQLAGSIVGVMFFFGESIVRYMLIFLSVGA"
BASE COUNT          759 a         1502 c         1481 g          839 t
ORIGIN      
        1 ggccctcggc tacaccgcgt ccgaagcgcg ttccgcagcc acggcggccc tgtccgcgtt
       61 gcccgtcggc gcgtcgctcg aggagcgcgt caaggcgtcg ctgcgtatcc tgcgtcggga
      121 ctgaccacaa ctaggaacga ctcccttgct tgaacacctc tcggtgatgc tgatcgccgt
      181 gatcgcgctc gcgatcgcgt tcgactacat caacggcttc cacgacacgg ccaacgcgat
      241 tgcgacgtcc gttgccacgc gcgccctcga tccgcgcgcg gcgattgcca tggcgacggc
      301 attcaacttc atcggcgcgt tcgcgggcac ggccgtggcc aagacgattg gctcggggct
      361 ggtcgatgaa tccaccagca cgcagggcgt tatcgcggca gccctgatcg gggccatcac
      421 ctggaacctg ttgacctggt attacggcct gcccagctca agcagtcatg cgctgatcgg
      481 cggactgttc ggtgccactc tcgtctcggc tggaccgggc gccttcaagc tcgacggcat
      541 agtgcaaaag gtcatattcc cgatgatcgc ctcgcccgtg gtcgggttct ttgtcgccct
      601 ggccctggtc gtgttgctgt tctggctttt cttccgggcg cagcgacggc cgatgacgac
      661 ggtcttccgg cgtctccaga tcgtctccgc cggattcatg gccttttcgc atggctcgaa
      721 cgacgcgcag aagacgatgg gcatcatcac cctgggcctg ttctcggcag gcgccttgtc
      781 caccaacgac gtgccgtggt gggtgatcgt cgttgccgcg accgcgatct cgctcgggac
      841 ggcggtcgga ggctggcgga tcatgcacac gatgggacac cgtgtggtga agctggagcc
      901 cgttcacggc ttcgcggccg agacaacggc ggcgacggtc atctttactg cagcccattt
      961 cgggatgccg gtgtcgacca ctcacgtcat ctcgagcgcg atcatcggcg tcggctcggc
     1021 gcggggacta cgccgcgtca gatggggagt ggcgcggagc atcctgctgg catggatcat
     1081 cacgatcccc gccgcagccc tggtcgccgc cggatcctgg ttcgttctac atctcgttgg
     1141 actcggctaa aggaggctcg cgtgccactc agtttgatcc cgaaggacac caaatttctg
     1201 gacatgttcg cgaccagtgg cgagaacctc gcggctgcga cacgggagct ggtcgagttc
     1261 gtcgacaagt tcgacaacct ggacgagcgc atcgccgaca tccaacggtt ggagaagcgc
     1321 ggcgaccaga tcgaccgcga gatcaaccgg cgtctggagg cggcgttcgt ggcgccgttc
     1381 gaccgcgagg acatccacga gttgaccgtg cggctcgacg acgtggtcga ctacgtccag
     1441 gcggtcggcg agagcctgat catctacgac gtcaaagcac cgacggacga gtgtcgccgc
     1501 ctggcgagca tcctcgccga ccagggacaa gagctatccg cagcgctcac gaagctcgac
     1561 aagatgaaag acctgagcgt ccacctcgag cgcatccacg acctggagca cgaggccgac
     1621 ggcgtctcac gcaaggcaat cggccggctc ttccgcgaga accacgaccc cctcgacgtc
     1681 atcaagtggc gcgagatcta cctcgagctg gagaacgcca tcgacgcggc cgaggacgcg
     1741 gctgaggcca tcgagaggat gatccacaag cgagtctgat tgctcgcttg catgtcgaac
     1801 gggcgttcta aactgccctc agactggtaa actcgacggc agatgcagga accggatcgc
     1861 agcgtcgatg gggacgcact gacgctcgaa gaagtcgctg tagagacatc tcttcgaccc
     1921 cggctcctgg cggagtacat cggccagtcc gccgtcaagc agaacctcga gattcttctc
     1981 tccgcagcgc gccaacgtaa cgaatcggcc gaccacgtcc tgctctacgg tcccccgggc
     2041 ctgggcaaga cgacgctggc aaacatcatt gcgcgtgagc tgggcgtgaa ccttcatgcc
     2101 acgtccggac cggcggttga gcgtcccggg gacctggccg cgatcctcac caacctcgag
     2161 gaacgcgacg tcctcttcat cgacgagatt caccgcctca accactccgt ggaagagatc
     2221 ctctacccgg ccatggagga cttcgccatc gacgtgatga tcggccgcgg gccctcggcg
     2281 cgcagtctgc gccttggact caagccgttc accgtcatcg gtgccacgac acgcgccggg
     2341 cggatcagcg ggccgctgcg cgataggttc ggcgcggtct atcggctcga ctactactcc
     2401 ggggaggacc tggccacgat cgtccgccgc tcggcgggga tcctcgcggt tgatatcgac
     2461 gacgccgccg tcaacgtgat cgcgcggcgg ggcaggggaa cgccacgtgt agtcaaccgc
     2521 ttgttgcgcc gagtgcgcga ttacgcccag gttcgctcgg acggacgcat cggcccgacg
     2581 gaagccgcgg acgcaatgaa cgccatggac atcgacgacg agggcctcga caccaccgat
     2641 cgccgtctgc tggccgccat cgtgcagaag ttcgcatcag gacccgtggg tggcgccgcc
     2701 gtggctgcgg tgatcggcga ggaggtcgac accatcgagg acgtctacga gccctaccta
     2761 ctccagctgg gcttcctgga ccgcactcca cagggccgaa tagcgaccga gcgagcacgc
     2821 gaccacctgc gcaggctcgg ctacgaagtt ccgactccgc gacgggcaga gcgttccgac
     2881 tccgggttgt gggatggcat caacgtcgag ggccgcaccg agtctgaaac cgttgcatga
     2941 ctgacgcggt catggagtcc tcggccccca ccgagcacgc agtggtgacc cgcgggctga
     3001 tgaagtccta cggccggcga gtcgcactga ccgggctcga catgtcggtt ccacgtggcc
     3061 agatctacgg tttcctcggc cccaacggta gtggcaagac cactgcgcta cggctgctga
     3121 cgggcctgtt gcgaccgaat ggcggcacca tcgagctctt cggtcagcct tactcgtgga
     3181 ccgatcggaa gcggctcttc cgcgtgggct cattgatcga gacgccgtcg ttctatccgt
     3241 acatgtcggg gcgcgagaac ctcctcgtgc tcggagcaag tggcccaccc acaccggcgg
     3301 cccgcatcga ccaggtgctg gaatacgtcg gcatgtcggg acgtgccaag gacgccgtgc
     3361 agacgtattc gctgggtatg aagcagcgcc tcggcatcgc cgcggccatg ctgtccgacc
     3421 ccgacctgtt gctccttgat gagcccgcca atggactcga tccgggcggc atggtggcga
     3481 tgcgcgaaac gcttcgttgg ttcacgcgcg agctgggcaa gacggtggtg atctcgagtc
     3541 accttctgca cgaggttgag cagttggcgg atgtcgtcgg gatcattgat agcggccgcc
     3601 tcatccgtga gggcaatctg cagaagctca tggaggacgc gggtcgcgtc aaggtgcgcg
     3661 tcgcgccgtc ggagatggca caggccgggt ccatcctccg ctcgttcgcg ccggatcggc
     3721 cgctgtacgg ggtcgactcc ggcgatgggg cgggttggtt cacggtgaac gtttcgccgg
     3781 cccgcgcagc agagatcaac aaggtgctgg ctgaggcggg tatctacgcc tccggccttg
     3841 agccgggcac ggatctcgag acggtcttcc tggagatcac aggctcgatc tcaggccacg
     3901 tgaacgcccc gccgacgatg cccggccaac cgccggcccc gccggccccg atgcccccgc
     3961 cagttccgcc aggcgcatga ggctcgtcaa ggcggagctg ctcaaggcgc gccgccgtag
     4021 cgccacctgg atcgtgctgg tcgtcacgct tgtgctgatg ggcgtcctct acctcttgtc
     4081 ggggcccgtg ttcaagggac ttggcgtgat cgagttccca caggcatacg tcgtcatcgg
     4141 ccagttcgca tatggcctcg gcggcctcct ggcactgatc tatgccgcag cgttcgtcgg
     4201 cgccgactgg aactggggcg tcatccgtaa cgtggtcgct cgcggggaga gtcgggaggc
     4261 ctaccttctg gccaaggccg cggcgcttgc catcctgctt ggaatcgcgc tcctgatcct
     4321 cttcctgttc ggcatcgtga tgtcctatgt cacgggcttc gcgtgggacg tgccggtcgc
     4381 cagtccgctt cgagcagagg gtctttcgga tctgctgacg tggatcggac tcggttttcc
     4441 cgttctcctg cagcgcgccg cgatcggttt cgcggtcgcg gtgatcctgc gcagccagct
     4501 ggccggttcg atcgtcggcg tcatgttctt cttcggcgaa tcgatcgtgc gctacatgct
     4561 gatcttcctg tccgttggcg c
//
LOCUS       DATLCO010000236         4605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8389713, whole genome shotgun sequence.
ACCESSION   DATLCO010000236 DATLCO010000000
VERSION     DATLCO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8389713"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VMZ33_05625"
     CDS             complement(<1..261)
                     /locus_tag="VMZ33_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16743.1"
                     /translation="MKLQTQAVLRRPDTAAPLQLAGVLVAGSFYPNRTQDAWGPLHLE
                     IGLVALAPLSSVVADDGAAAAGQALVEAREQWSHPLLSRADVL"
     gene            568..951
                     /locus_tag="VMZ33_05630"
     CDS             568..951
                     /locus_tag="VMZ33_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUR16744.1"
                     /translation="MADAYQRLIAVWNGGSTDALPSIVSDDYRGHMLHLPAGERDAET
                     YAAWVHDFRKANPTTRFEVVEQTFAGDQVVSRLVATRHNPGLGPEFANGINICRVDPK
                     GLVAEEWAIWSTWMPSGVSEVVSGE"
     gene            1112..3100
                     /gene="ftsH"
                     /locus_tag="VMZ33_05635"
     CDS             1112..3100
                     /gene="ftsH"
                     /locus_tag="VMZ33_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HUR16745.1"
                     /translation="MNSRFLRNGIVTLVLVVGTAALLYMFVFPSQNPSLVPYSGGESS
                     FERLVMDGKVAKVTQRGQQLEIELKQLDANSKPLLSTSNVPSEFRTDVATDVTTWCAS
                     APTTCAAAPAVTGDRPSDSGAWLGILLSALLPILLIGALLFFMFRQAQGTNNQAMSFG
                     KSRARMFLGNKTVVTFQDVAGVDEAKTELQEVVEFLKYPEKFNSLGARIPRGVLLVGP
                     PGTGKTLLARAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNSPCIVFVD
                     EIDAVGRQRGAGLGGSHDEREQTLNQILVEMDGFDTNTNVIVVAATNRPDVLDPALLR
                     PGRFDRQVILDRPDMKGRLEILKVHIKGKPLDKQVELNNVARSSPGFSGADLANLVNE
                     AAILAARRNKKTIGMAEFQEALERVVAGPERKSRVISDAEKAIIAYHEGGHAIVQRIL
                     PKTDPVSKVSIISRGMALGYTMGLPQEDRYLQSKSEFEDKIAGMLGGNASEKLIFGDT
                     TTGSSNDIEKATTLARRMVTEFGMSEKLGPLAFGKRDELVFLGREIGEQRNYSDDVAK
                     MIDEEVRAIIDRGYSKAMDVLVRYKDRLIQLAERLVAEETIEQEAFEQMFADIPDPRN
                     DTHMTPTPLDGSRPRRAPAEEEPSPPPTAAPNPSPQPA"
     gene            3161..>4605
                     /locus_tag="VMZ33_05640"
     CDS             3161..>4605
                     /locus_tag="VMZ33_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16746.1"
                     /translation="MSDLTTRSLVGAWLLDRTGDWLALITILVAAAELSVDNASAIGW
                     ALGLWVAPRMVIGILYASVGDKLGERAWPALALAKAVLIVLVFGVAVATPLEPESAEW
                     LLGTAAVLGFAAALTTESRALVLRGSFGIERVGQLSAAASFVDRLAVLLAGALAAVVG
                     YVAPVGIALGLAIVAFAAAAVLAYSALQRARGQSRANLVPTPVEEPDPRETARQTVLW
                     LITAFTSGAVGAAFLVTAFTFFIDVSVGGDGFRPESGGALLVAATGLGMALGPLPMPR
                     LLLRLPAALILVGLALSVAVAAVVLAVAPSLPVALAAFAAFGFTAVSIDRLRSIALKR
                     VVPPTLSPRLVRMSILAVSAGQLFGALGIVSTPGREPIEIVVFLAVAQLVLVGLGAVR
                     GGRGALRVGSLSPLAIKSVVHKLSWDTDPPAQALNSEDLEPRVRKLARWINRTVDLQR
                     LTAVLPISKREYTVFRPDDVSRESLFEAGRA"
BASE COUNT          778 a         1529 c         1495 g          803 t
ORIGIN      
        1 gagcacgtcc gcgcggctta gaagcgggtg actccattgt tcgcgcgcct cgaccaacgc
       61 ctggccagcg gccgccgctc catcatccgc gacgacggag ctgaggggag caagtgcgac
      121 caggccgatt tcgagatgca acggacccca ggcgtcctgt gtgcgattgg ggtagaagga
      181 tccggcgacc agcacacccg cgagttggag tggcgccgca gtgtcagggc gtcgaagtac
      241 cgcctgagtc tggagcttca tgggcgggtg cgggcgcagg agttgcccgg cgcgttatcg
      301 catcgggcgt gccggcgacc gcggtgcatg ctgttcatcg cgttctccga taccgacgca
      361 cggacgtcgg tatccactgg aagcgcttgg agcatctcgc gtcacacgaa gcgagggaca
      421 caccagttgt cttcgagcca gatcacaccg tacggacagg ctgacgcgct gtgggtgaca
      481 gggttctggt gttaggttgc gaaggcgttg cgcccgtcgc agcgtagaaa atgtcgcagc
      541 gtcgactgac gaggagttaa ccacgcaatg gcggacgctt atcaaaggct tattgccgtc
      601 tggaacggag gctcgacaga cgctctgcct tcgatagtct ctgacgacta ccgcggtcac
      661 atgctccacc tgccggctgg tgaacgggac gccgagacgt acgcggcgtg ggtccatgac
      721 ttccggaaag ccaatccaac caccaggttc gaggttgttg agcagacctt tgccggcgat
      781 caggttgtca gccgactcgt tgctacgcga cacaacccgg gtcttgggcc tgaatttgcg
      841 aacggcatca acatctgtcg ggtcgacccg aaaggcctag tcgccgagga gtgggcaatt
      901 tggagcacct ggatgccaag cggcgtcagc gaggtcgtgt cgggcgaata gaccctgtcc
      961 agacccttgt gacgctggcc gtccgccgcc gaaccctctg cacgccgagt tgcctcggtg
     1021 agcacgaaat ggggaagcag acccccgcgc ctataatcag gtgaccgggg cgcgtcacgc
     1081 gcgctcggtc aaccccggag gcatttcccc tttgaacagt cggtttctac gcaacggcat
     1141 tgtcacgctc gtgctcgttg tcggcactgc ggcgctcctc tacatgttcg ttttccccag
     1201 ccaaaacccg tcactcgtcc cgtacagcgg cggcgagagc tccttcgaga ggctcgtaat
     1261 ggacggcaag gtggccaagg tcacgcagcg cgggcagcag ctagagatcg agctcaagca
     1321 gctcgacgcg aatagcaagc cgctcctcag caccagcaac gtacccagcg agttccgcac
     1381 ggacgtggcc acggacgtca ctacctggtg tgcttcggca cccacgacct gtgccgcggc
     1441 tcccgccgtt acgggagata ggccgtcgga cagcggcgcg tggctgggca tcctgctgag
     1501 cgccctcctg cccatcctgc tgatcggcgc cctgctgttc ttcatgttcc ggcaggccca
     1561 gggcaccaac aaccaggcca tgtccttcgg caagagccgc gcccggatgt tcctcggcaa
     1621 caagacggtc gtgacgttcc aggatgtcgc cggcgtcgac gaggccaaga ccgagctgca
     1681 ggaagtcgtc gagttcctca agtaccccga gaagttcaac tcccttggcg cgcgcattcc
     1741 gcgtggcgtg ctcctcgttg gcccaccagg tacgggcaag acgctcctcg cgcgagcagt
     1801 tgcaggcgag gcgggcgtgc cgttcttcag catctccggt tccgagttcg tcgagatgtt
     1861 cgtcggcgtc ggtgcgagcc gcgtccgcga cctcttcgac caggccaagc gcaattcacc
     1921 gtgcatcgtc ttcgtggacg agatcgacgc tgtcggccgc cagcgcggcg ctggcctcgg
     1981 tggctcgcac gacgaacgtg agcagacgct caaccagatc ctcgtcgaga tggacggctt
     2041 cgacaccaac acgaacgtga tcgttgttgc cgcaacgaac cgcccggacg tcctcgaccc
     2101 ggcgctcctg cgccccggcc gtttcgaccg ccaggtcatc ctggaccgac ccgacatgaa
     2161 gggccgcctc gagatcctca aggttcacat caagggcaag ccgctcgaca agcaggtcga
     2221 gctcaacaac gtcgcccgct cgtccccggg cttctctggt gcagacctgg ccaacctcgt
     2281 taacgaggca gccatcctgg ccgcgcggcg caacaagaag acgatcggca tggccgagtt
     2341 ccaggaggcc cttgagcgcg tcgtggccgg ccctgagcgc aagagtcgcg tcatcagcga
     2401 cgccgagaag gcgatcatcg cttaccacga aggcggtcac gccatcgtgc agcgcatcct
     2461 ccccaagacc gacccggtca gcaaggtcag catcatcagc cgcggcatgg ccctcggcta
     2521 cacgatgggg ctgccgcagg aggaccgcta tctccagagc aagagcgaat ttgaggacaa
     2581 gatcgcgggc atgctcggtg gtaacgcttc ggagaagctg atcttcggcg acacgacgac
     2641 cggctccagc aatgacatcg aaaaggccac cacgctggcc cgacgcatgg tcacggagtt
     2701 cggcatgagc gagaagctcg gaccgctggc ctttggcaag cgcgatgagc tcgtgtttct
     2761 gggccgcgag atcggcgagc agcgcaacta ctccgacgac gtcgcgaaga tgatcgacga
     2821 ggaagtgcgc gcgatcatcg atcgtggcta ctccaaggcg atggacgtgc tggtgcggta
     2881 caaggaccgc cttattcagc tcgccgagcg gctcgtcgcc gaggagacca tcgagcagga
     2941 agcgttcgag cagatgttcg ccgacattcc ggacccccgg aacgacacgc acatgacgcc
     3001 gacgccgctc gacggctccc gtccgcgccg ggcaccggca gaggaagaac cgagtcctcc
     3061 gccgaccgcg gcgccgaacc cttcgcccca gcccgcgtag gccacacaac cgaccgatag
     3121 ccagacagtg ctggctgaac ttcggcgcgc catacgcgcc atgagcgatc tcacgacccg
     3181 gtcgctcgtt ggcgcctggc tcctggaccg caccggcgac tggctggcgc tgataacgat
     3241 ccttgtcgcc gcggccgagc tgtccgtcga caacgcgtcg gcgatcggct gggcgcttgg
     3301 cctgtgggtg gcgccgcgca tggtcatcgg catcctgtat gccagcgtcg gtgacaagct
     3361 cggcgagcga gcgtggccgg ctctggcatt ggccaaggcg gtgctcatcg tgctggtctt
     3421 cggcgtcgct gtcgccaccc cgctcgaacc cgagtcagcg gagtggctgc tcgggacagc
     3481 tgccgtactg gggttcgctg ccgcactgac taccgaatct cgcgccctgg tgctgcgcgg
     3541 cagcttcggc atcgagcgcg ttgggcagct gtcggccgcc gcttccttcg tggatcggct
     3601 ggccgtcctg cttgcaggcg cgcttgcggc ggtggtcgga tacgtggccc cggtcggcat
     3661 cgcgctcgga cttgcgatcg tggctttcgc ggcggccgcc gtgctcgcct acagcgcgct
     3721 ccagcgggca cggggccaga gccgcgcgaa cctcgtaccg acgccagtcg aggagccaga
     3781 cccgcgcgaa accgcgcgcc agaccgtctt gtggctgatc acggcattca cgtcgggcgc
     3841 ggtcggcgcc gcattcctcg taacggcgtt cacgtttttc attgacgtct cggtgggtgg
     3901 ggacggcttc cggcctgagt caggcggcgc gttgctggtg gccgcgaccg gcctcggaat
     3961 ggcactcggc ccgctgccca tgccgcggct gcttctgcgg ctccccgccg cgctgatcct
     4021 ggtcggcctg gcgctgtcgg tggcagtcgc ggccgtggtc ctcgccgtgg ccccgtcgct
     4081 gcccgtggct ctcgctgcgt ttgcagcctt cggcttcacg gcggtgagca tcgaccgtct
     4141 cagatccatc gccttgaagc gagtcgtgcc gccgacacta tccccgcgac tcgtccggat
     4201 gtcgattctc gcagtcagcg ccgggcagct gtttggcgcc ctcggcattg tcagcacgcc
     4261 gggtcgcgag cccatcgaaa ttgtcgtctt cctggcagtg gcgcagttgg tgttggtcgg
     4321 cctcggtgcc gtccgtggcg gccgcggcgc actcagggtc ggaagcctca gcccgctcgc
     4381 gatcaaatcg gtcgtccata agctgtcgtg ggataccgat ccaccggcac aggcgttgaa
     4441 ctccgaagac ctggaacctc gcgtccgaaa gctcgcccgg tggatcaatc ggacggtcga
     4501 tctccagcgc ctgacggcag tccttccgat atcgaagcgc gagtacacgg tattccgccc
     4561 cgatgacgtt tctcgcgaga gcctgtttga agcgggtcgc gccga
//
LOCUS       DATLCO010000237         3050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4094348, whole genome shotgun sequence.
ACCESSION   DATLCO010000237 DATLCO010000000
VERSION     DATLCO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4094348"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VMZ33_05645"
     CDS             complement(<1..755)
                     /locus_tag="VMZ33_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HUR16747.1"
                     /translation="MTLNRSIVLLVVALLIAACGGGGATTRPSSAPSTAASSAPTNAV
                     SNAPTEAPATATPVAATPTAVADACADVDALLKNSGRLTLSTDIPAFGPWWGGDAATQ
                     YDNEPEGGSPWTETDFSAEPYSGEGFEGALAYAVAEAMGFEADQVDWLANPDFALAFA
                     PGEKEFDFHMAQISASADRAEAVTFSDPYFDSNQAVLALTTNDISAATSIEELKAFRL
                     GAAANTTSFTFLEDSIAPDVEPQVFTDNAGTLQA"
     gene            complement(810..1169)
                     /locus_tag="VMZ33_05650"
     CDS             complement(810..1169)
                     /locus_tag="VMZ33_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16748.1"
                     /translation="MIRDRFALGITGLSVGALTTLAALAMNDDSPRNIDVVVLWLAGA
                     MHLGLLVTWALVSRLPKWIVAGWGLILIATGIGVIITAPVAAGASSMPWALLGLVVVG
                     IVTIVTAIVHSGEPTSH"
     gene            complement(1189..1710)
                     /gene="paaD"
                     /locus_tag="VMZ33_05655"
     CDS             complement(1189..1710)
                     /gene="paaD"
                     /locus_tag="VMZ33_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HUR16749.1"
                     /translation="MVAPTLATRPLESAVWAALDDVHDPEIPTISVVDLGVVRSVQTS
                     DNSIRVELMPTFVGCPAVEVMRQSVADRLHEFADDVRVDITFAQPWTTERITPQGRAA
                     LRQAGFAPPHLALGAADSDRFLSVLPSLPVGECPYCGSRNTSLENAFGPTLCRAIYHC
                     GDCSQPFEQFKEV"
     gene            complement(1704..2540)
                     /gene="paaC"
                     /locus_tag="VMZ33_05660"
     CDS             complement(1704..2540)
                     /gene="paaC"
                     /locus_tag="VMZ33_05660"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HUR16750.1"
                     /translation="MRTDARLPTRQLIQPDLPAFDVGSLAPDLRGAVADLLLTMADDE
                     FVIGFRDSEWTGIAPILEEDVAFSSLAQDEIGHARVWYEMRGQIVGEDPDRTAFQREP
                     NEYRHAKLIDHERQGWGFTITRRWLYDTADAVRLAALAESSFAPLREVVAKVRREERY
                     HLMHLEAWMGRLSARGGLPRGKVLSALKHLAPVSLSVFTPLASEAALINAGVLGSPMS
                     ELAARWRSDVNSRLQSWGLRLIPDGSPPASGRDHAQPSEAFQSLWNEFTAVARLEEGA
                     AW"
     gene            complement(2537..2920)
                     /locus_tag="VMZ33_05665"
     CDS             complement(2537..2920)
                     /locus_tag="VMZ33_05665"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16751.1"
                     /translation="MTARQLPTPSEGRVAEDGRLGEGADPEVWEVFRQEKDGDPMRHA
                     GNVRAPDRDLALHYAREFYSRRQESIRLWVVRRTEITDLSDPDLLTPPLDRSFKKPGG
                     YVMRDKLEVAKKRAAEAKPTDTQAR"
BASE COUNT          518 a          992 c         1025 g          515 t
ORIGIN      
        1 gcctggagcg tcccggcgtt gtccgtgaag acctgcggct caacgtccgg cgcgatcgag
       61 tcctcgagga atgtgaagct ggtcgtgttc gctgctgcgc ccaaccggaa agccttcagc
      121 tcttcgatcg aagtggcggc actgatgtcg ttggtcgtga gcgcaagcac ggcctgattg
      181 gagtcgaagt aggggtcgct gaaggtaacg gcctcggctc ggtcagctga tgccgagatc
      241 tgggccatgt ggaagtcgaa ttccttctca ccgggcgcga aggcaagggc gaagtccggg
      301 ttggccagcc agtcgacctg gtccgcttcg aagcccatcg cctcggccac ggcgtaggca
      361 agcgcgccct cgaagccctc gcctgaatag ggctcggcgc tgaagtcggt ctcagtccac
      421 ggcgagccac cctcgggctc gttgtcgtac tgcgtggcgg catcgccgcc ccaccatggc
      481 ccgaaggccg ggatgtcggt gctgagtgtc agccggccgg agttcttcag caacgcatcc
      541 acgtccgcac aggcgtctgc gacggccgtc ggtgtcgccg cgacgggtgt ggccgtggcc
      601 ggcgcctcag tcggggcatt gctgactgcg tttgtcggtg cactcgatgc ggcggtgctc
      661 ggcgcgctcg atggacgggt ggttgctccg cctccgccgc acgctgcaat gagcaacgcg
      721 acgacgagca aaacgattga gcgattaagg gtcacgacgg gtcctcctcg atgttgggcc
      781 cgaggatagc gaaaaccgga gcgccaccct cagtggctag ttggttcccc gctgtggacg
      841 atcgctgtga cgatggtcac gatgccgacg acgaccaacc cgaggagcgc ccagggcatg
      901 ctcgaagcgc ccgctgccac cggcgccgtg atgatcacgc cgatcccggt agcgatgagg
      961 atcaagcccc agcccgcgac gatccacttg ggcagccgcg aaacgagtgc ccaggtgacg
     1021 agcaagccga ggtgcatggc gcccgccagc cacaacacga cgacgtcgat gttgcgcggc
     1081 gaatcgtcgt tcatcgccag tgcggctagg gtcgtcaacg cgccgaccga aagtccagtg
     1141 ataccgagcg cgaatcggtc gcggatcaag cttctctaga cctccgactc agacttcctt
     1201 gaattgctca aaaggctggc tgcagtcgcc gcagtggtag atcgcgcggc acagtgtcgg
     1261 gccgaacgcg ttctccagcg acgtgttgcg tgagccgcag tatggacact ccccgacggg
     1321 cagactcggg agaactgaca ggaatcgatc gctgtccgcc gcgcccaggg ccagatgcgg
     1381 cggcgcgaaa ccggcctggc gcagcgccgc ccgaccctgc ggcgtaatcc gctccgtcgt
     1441 ccatggctgg gcgaaggtga tgtcgacccg cacgtcatcg gcgaattcgt gcagccgatc
     1501 agcgacagat tggcgcatga cctcgactgc cggacaaccc acgaacgttg gcatgagctc
     1561 gacgcggatc gagttgtcag aggtctgaac gctgcgaacg actccgaggt ccacgaccga
     1621 gattgtcggg atctccggat cgtgcacgtc atccagagct gcccaaacag ctgactccag
     1681 tggcctcgtt gccaaagttg gtgctaccac gcggcgccct cctccagccg ggctacggcg
     1741 gtgaactcgt tccacagcga ctggaaggct tcgctcggct gagcgtgatc gcgaccgctg
     1801 gctggtggcg agccatctgg tatgaggcgc aaaccccagg actggagacg actgttgacg
     1861 tccgatcgcc agcgggcggc cagctcggac atcggcgacc ccaagactcc tgcgttgatc
     1921 aacgctgctt ccgacgcgag cggtgtgaag accgacagcg ataccggcgc caggtgcttc
     1981 agggccgaga ggaccttgcc gcgcggcagg ccgccccgcg cagagagccg ccccatccac
     2041 gcctcgaggt gcatgaggtg gtagcgctcc tcccgccgta ccttggcgac gacttcgcgc
     2101 aagggcgcaa agctcgactc ggcgagggcg gcgaggcgga cggcatccgc ggtgtcgtac
     2161 agccagcgcc gcgtaatcgt gaagccccag ccctggcgct cgtggtcgat cagcttggcg
     2221 tgccggtatt cgttcggctc gcgctggaac gccgtgcgat cgggatcctc gccgacaatc
     2281 tggccacgca tctcgtacca gacgcgtgca tgaccgatct cgtcctgcgc caaagacgag
     2341 aacgcgacgt cctcctcgag gatgggtgcg atgccggtcc actccgagtc gcgaaaaccg
     2401 atcacgaact cgtcgtcggc catggtcagc aacaggtcgg ctaccgcacc gcgcaggtcg
     2461 ggtgcgagcg agcccacgtc gaacgccggc aggtcgggct ggatcagctg ccgcgtgggc
     2521 agccgcgcgt ctgtcctcat cgggcttgcg tgtcggtcgg tttggcttcc gcggcgcgct
     2581 tcttggcgac ctcgagcttg tcgcgcatca cgtacccgcc gggcttcttg aaggagcggt
     2641 cgagtggcgg cgtcaggagg tcggggtccg agagatcggt tatctccgtc cggcgaacga
     2701 cccataggcg gatcgactcc tggcggcggc tgtagaactc gcgcgcatag tgcagcgcca
     2761 aatcgcggtc gggcgcccgg acgttgcccg cgtggcgcat cgggtcgcca tccttctcct
     2821 ggcggaaaac ctcccaaacc tccggatcgg ctccctcgcc gagtcgaccg tcttcggcga
     2881 cacggccttc gctcggagtg ggaagctgtc gtgcggtcat gagcgtggct caggcagctg
     2941 ccgctgcgcg cggcgacagg accatgtccc gtacccagga tgtctgctcg tgattgagac
     3001 ggcggaaatc cagtcgctcc tgtgacttcg gcccgtggcc ggtgactacg
//
LOCUS       DATLCO010000238         5647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7644953, whole genome shotgun sequence.
ACCESSION   DATLCO010000238 DATLCO010000000
VERSION     DATLCO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7644953"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /gene="zwf"
                     /locus_tag="VMZ33_05670"
     CDS             complement(<1..1091)
                     /gene="zwf"
                     /locus_tag="VMZ33_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HUR16752.1"
                     /translation="MATTTSRRQVRTASGTSKSEPSTEAVHAQRAKGEEPENPLRAGL
                     ALERVPDPCVIVVFGATGDLTARKILPAVYNLRRSGLLPPETTVLGYSRRPLSDDDFR
                     KLARESLDAHSRVKVEPGLWDDFMEGVFYQPGDFADRGAYKDLAERLEQIDAARGTRG
                     NRLFYLATPPSAYTDIISNLGRVGLDKQGEREGWARLVIEKPFGHDLESAVKLNDEIN
                     KVLDESQIYRIDHYLGKETVRNLLVFRFGNGIFEPLWNRRYVDHVQITVAEDLGVEGR
                     GSFYEEAGASRDILQNHLLQLLTLVAMEPPIAFEADALRDEKVRVLRAIETNWTEARA
                     RAQVVRGQYTSGWVGDKHVAGYREEEEVDA"
     gene            complement(1102..2004)
                     /gene="gnd"
                     /locus_tag="VMZ33_05675"
     CDS             complement(1102..2004)
                     /gene="gnd"
                     /locus_tag="VMZ33_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HUR16753.1"
                     /translation="MELVICGLGRMGGNMARRLHNAGHRVIAYNRSFDKTEEIMGEGL
                     EGAREPADVVAMLTAPRIVWLMVPAGDATEQSLLEFADLLEPGDTIVDGGNSNFKDSK
                     RRHAMLAERGLKFVDAGISGGVWGLTNGYGTMVGGDPDAVTSLEPIFTALAPPDGGYV
                     YCGPSGAGHYTKMVHNGIEYGLMQAYAEGFEIMHASEYPLDLEKIAKAWMHGTVIRSW
                     LLELAGLAFEQGGTELEGIKGWVADSGEGRWTVQDAIDHDVPAPIITLSLLARFASRQ
                     DESYSAKVTAALRQQFGGHAVKSE"
     gene            complement(2018..3775)
                     /locus_tag="VMZ33_05680"
     CDS             complement(2018..3775)
                     /locus_tag="VMZ33_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16754.1"
                     /translation="MARGSRLPADLQERVDGMLARALAEDWVTRLWARDASLWTDDDH
                     VSSLIANRLGWLDLPRTFADRAGTLEAFAQGVRSEGFTAAVVCGMGGSSLAPEVLALS
                     LPLGDTGIPVRVLDSTDPTAVRAASSASDPASTLYLIASKSGTTTESLAFLAHFWKLE
                     DDIHSDVPQGLAGAHFAAITDPGKSVDAIPNTDVFREVFLNPEDVGGRYSALTYVGLV
                     PAALMGLDTRSLLDDAARMAALCRTTEQTNPGLWLGVAMGTLAAAGRDKLTIIIEPRV
                     ASLGGWIEQLVAESTGKHGVGVVPVATEFPTDPSVYDDDRVFVRLSSGTDASWQAATD
                     AAMSALADAGHPVIELELGSGPGALGAEFFRWEFATAVAGAVLGINPFDEPNVTESKE
                     NTKRVLDTFQESGSVPAQEVLAADGTLALAGDAPLRLTAASAGSGDLAQELRRHLTRC
                     RPGGYYGIHAYVAQTPERDQALAGIAQLLRDRTKRAVTVGYGPRFLHSTGQLHKGGTP
                     SGCFLQLVTDYSPDDDLEIPGKNETFATLVAAQAAGDFISLESHDLPVATVNLSADRD
                     AGLARLREALDQALAQPLE"
     gene            complement(3790..>5647)
                     /locus_tag="VMZ33_05685"
     CDS             complement(3790..>5647)
                     /locus_tag="VMZ33_05685"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HUR16755.1"
                     /translation="MTDALPDRQTWIDLARQLRIDSIRVSSAAKSGHPTSSMSAAELM
                     AVLVAKYWRYDVANPRAPHNDHLIFSKGHASPLFYAILKGIGAVDEQELMTYRMRGSR
                     LEGHPTPILPYVDVATGSLGQGLPIGVGIALAGKKLDKLPYRTWVLCGDSEMAEGSIW
                     EALAQASFDNLDNLTAIIDVNRLGQTGPTRYGWDLDVYSDRLRSFGWHAIEVDGHDVD
                     AVDRAYAEAVATTGRPTAIVARTVKGKGVSAVADQLGAHGKAVDDESAALGELGDSPN
                     MIVTLHKPDTGTAPNKFASAGELDLPRYELGQKIATRKAFGDALAALGRADGDVVVLD
                     GEVGNSTYTETFGKEMPDRFFQMYIAEQQMIGTAVGMQVRGWKPFAATFAAFLTRAYD
                     FIRMGAVSRANLAICGSHAGVSIGEDGPSQMGLEDLAAMRAVHGSTVLYPSDANATAK
                     LVALMADQPGISYIRTTRQATAVIYGPDEEFRIGGSRVVRVGHDDQLTIIGAGITLSE
                     ALTAADTLAGDGINARVVDLYSIKPLDTETLITAARETGRILTVEDHWPEGGIAEAVF
                     SALAESGVPFLAASLAVRNMPASATPEEELADAGIDAKAIAQAARLLAQRTA"
BASE COUNT          893 a         1852 c         1927 g          975 t
ORIGIN      
        1 gcgtcgacct cctcctcctc gcgatagccg gcgacgtgct tgtcaccaac ccagcccgac
       61 gtgtactggc cacgcacgac ctgggcgcgc gcccgggctt cagtccagtt cgtttcgatt
      121 gcgcgcagga cacgcacctt ctcgtcgcgg agggcgtcgg cctcgaaggc gatgggcggc
      181 tccatcgcca cgagagtcaa tagctgcagc aggtggttct ggaggatgtc gcggctcgct
      241 ccggcttcct cgtagaagga gccgcggccc tcgacgccga gatcctctgc caccgtgatc
      301 tgcacgtggt cgacatagcg gcggttccac agcggctcga agatgccgtt gccgaagcgg
      361 aagacgagga ggttgcggac tgtctccttg cccaggtagt gatcgatgcg gtagatctgc
      421 gactcgtcga ggaccttgtt gatctcgtcg ttcagcttta ccgccgactc gaggtcgtgg
      481 ccgaacggct tctcgatgac caggcgcgcc cagccctcac gctcgccctg cttgtcgagc
      541 ccgacacggc ccaggttgga aatgatgtcc gtgtatgccg acgggggcgt ggcgaggtag
      601 aagagccggt tgccgcgtgt gccacgcgcg gcatcgatct gctccagccg ctcagccagg
      661 tccttgtacg cgccgcggtc cgcgaagtcc ccgggctggt agaagacgcc ttccatgaag
      721 tcatcccaca ggccaggctc gaccttgacg cgggagtggg cgtcgagcga ttcgcgggcc
      781 agcttgcgga agtcgtcgtc gctcagcggg cgacgcgagt agcccaggac cgtcgtctcg
      841 ggcggcagca agcctgatcg acgcaggttg tagacagctg gcaggatctt ccgcgccgtc
      901 agatcgcccg tcgcaccgaa gacgacgatc acgcacggat caggcacccg ttccagcgcc
      961 aggccggcgc gaagtggatt ttcgggctct tcgcctttgg ctcgctgggc gtggacggcc
     1021 tcggtcgaag gctccgactt gctcgtcccc gaggcagttc gtacctgccg ccggctcgtg
     1081 gtcgtggcca ccttgtgcgt gctactccga cttgaccgcg tgaccgccga actgctggcg
     1141 gagagcggcg gtgaccttcg ccgagtagct ctcgtcctgg cggcttgcga agcgggcgag
     1201 aaggctgagc gtgatgatcg gggccggcac gtcgtggtcg attgcgtcct ggacggtcca
     1261 acgcccctcg cctgagtcgg ccacccagcc cttgatgccc tccaactcag tgccaccctg
     1321 ttcgaaggcg aggccggcaa gctcgagcag ccacgaacgg atgaccgtgc cgtgcatcca
     1381 ggccttggcg atcttctcga ggtcgagcgg gtattccgag gcatgcatga tctcgaagcc
     1441 ctcggcatag gcctgcatca gcccgtactc gatcccgttg tggaccatct tcgtgtagtg
     1501 gccggcaccc gacggtccgc aatagacgta ccccccgtcc ggtggcgcga gggccgtgaa
     1561 gatcggctca agggacgtca ccgcatccgg gtcgcctccg accatcgtgc cgtagccgtt
     1621 ggtgaggccc cagaccccgc cgctgatgcc ggcgtcgacg aacttcaggc cgcgctccgc
     1681 cagcatggcg tgacggcgct tggagtcctt gaagttggag ttgccgccgt cgacgatggt
     1741 gtcgccgggc tccaggagat cggcgaactc gaggagcgac tgttctgtcg cgtcaccggc
     1801 ggggaccatc agccagacga tgcgtggcgc ggtgagcatc gccaccacgt cggcgggctc
     1861 gcgggcacct tccaggccct cgcccatgat ttcctcggtc ttgtcgaacg accggttgta
     1921 ggcgatgaca cggtgtccgg cgttatgcag ccggcgcgcc atgttgccgc ccatccgacc
     1981 gaggccacag atgacgagtt ccactcttct tgctacctca ttccagtggt tgtgcgagtg
     2041 cctggtcgag cgcctcacgt agccgggcca gcccggcgtc gcggtcggcg ctgagattga
     2101 cggtcgccac gggcagatcg tgcgactcga gcgaaatgaa gtcgccggcc gcctgtgcgg
     2161 caacgagggt cgcgaaggtc tcgttcttgc ccgggatctc gagatcgtcg tcgggcgagt
     2221 aatcggtcac cagctgaagg aaacagccac tcggggtgcc gcccttgtgc agctggcccg
     2281 tcgagtgcag aaaccgcggc ccgtagccga cggtcacggc acgcttcgtc cgatcgcgca
     2341 gcaactgcgc gatgccggca agcgcctggt cgcgctccgg tgtctgcgcc acgtatgcgt
     2401 ggatgccgta gtagccaccc ggacgacagc gggtcagatg gcggcgcagc tcctgcgcca
     2461 aatcgccact gcccgcgctt gcagccgtca gccgcagagg cgcgtcaccg gcgagtgcca
     2521 gtgtgccgtc cgcagcaagc acctcctggg cagggacgga gccactttcc tggaacgtgt
     2581 cgaggacgcg cttggtgttc tccttggact ccgtgacgtt gggctcgtca aacgggttga
     2641 tgccaagcac cgcgccggcc accgccgtgg cgaattccca tctaaagaac tcagcgccga
     2701 gggcgccagg gccgcttccc agctccagct cgatgaccgg atggcccgca tccgcgagcg
     2761 cggacatggc agcgtcggtc gccgcctgcc acgaggcatc cgtacctgag ctgaggcgga
     2821 cgaagacccg gtcgtcgtcg tagacggatg ggtcggtcgg gaactccgtc gccaccggca
     2881 cgactccgac accgtgcttg ccggtgctct ctgcgacaag ctgctcgatc cagccgccca
     2941 gtgaggcaac gcgcggctca atgatgatgg tcagcttgtc gcgaccggca gcggcgagcg
     3001 tgcccatcgc gacgccgagc cacaagcccg ggttggtttg ttctgtggtg cggcagagtg
     3061 ctgccattcg tgcggcgtcg tcgaggagcg aacgagtatc aaggcccatg agcgccgccg
     3121 gcacgaggcc gacgtatgtc agggcgctgt agcggccgcc gacgtcttca gggttgagga
     3181 acacctctcg gaaaacgtcc gtgttcggga tcgcatcgac gcttttgccg gggtccgtga
     3241 tcgccgcaaa gtgcgctccc gccaggcctt gcggcacgtc cgagtggatg tcgtcctcaa
     3301 gcttccaaaa gtgagccagg aacgccagcg attcggttgt cgtccccgac ttggacgcga
     3361 tcagatagag cgtgctggcg ggatcggacg cgcttgatgc ggcgcggact gccgtgggat
     3421 cggtcgaatc cagcacgcgg acggggatgc cggtgtctcc gagcgggagc gacagcgcca
     3481 gaacctccgg cgcgagcgat gaaccaccca tgccgcagac gacggccgcg gtaaaaccct
     3541 cgctgcggac gccctgcgcg aaagcttcga gcgtccctgc gcggtcggcg aaggttcgcg
     3601 gcagatcgag ccaccccaat cggttggcga tcaatgatga gacgtggtca tcgtcagtcc
     3661 acaggctggc gtcgcgtgcc cacaggcggg tgacccagtc ctcagccaga gcccgggcga
     3721 gcatgccgtc gactcgctcc tggaggtccg cggggaggcg cgacccgcgg gccatggtcg
     3781 cgttctgcat cacgcggtcc gctgcgcgag caggcgcgcg gcttgggcga tggccttcgc
     3841 gtcgatcccg gcatcggcca gctcctcctc cggagtcgcg gacgcgggca tgtttcgaac
     3901 tgcgagagac gccgcgagga aaggcacgcc ggactcggcg agcgcgctga acacggcctc
     3961 ggcgatgccg ccctcaggcc agtgatcctc cactgtcagg atgcgccccg tttcccgtgc
     4021 ggccgtgatg agcgtctcgg tatcgagcgg cttgatcgag tagagatcga ccacccgggc
     4081 gttgatgccg tcgccagcga gggtgtcggc ggcggtgagc gcctcagaca gggtgatgcc
     4141 ggcgccgatg atggtcagct ggtcgtcgtg ccccacgcgc accacgcgac tgccgccgat
     4201 gcggaactcc tcgtcgggcc cgtagatgac cgccgtcgcc tgccgggtcg tgcggatgta
     4261 gctgattccg ggttgatcgg ccatgagggc cacgagcttg gcggtggcat tcgcgtccga
     4321 cgggtagagg actgtggagc cgtggacagc tcgcatcgcc gccaggtcct caaggcccat
     4381 ctgcgatggc ccgtcctcgc cgatgctcac acccgcgtgc gagccacaga ttgcgaggtt
     4441 ggcgcgcgac accgcgccca ttcgaatgaa gtcgtaggcg cgcgtgagga atgccgcgaa
     4501 cgtcgccgcg aatggcttcc agccgcggac ctgcatgccc acagccgtgc cgatcatctg
     4561 ctgctcggcg atgtacatct ggaagaagcg gtcgggcatc tcctttccga acgtctccgt
     4621 gtaggtcgag ttgccgactt cgccgtccag cacgacgacg tcaccgtcag ccctgcccaa
     4681 ggcggcgagc gcgtcgccga aagccttgcg cgtggcgatc ttctggccga gctcgtagcg
     4741 cggcaagtcc agctcaccgg cagacgcgaa cttgttgggc gccgtgccgg tgtcgggctt
     4801 gtggagggtc acgatcatgt tcggcgagtc gcccaactcg cccagagcgg ccgattcgtc
     4861 gtctacggcc ttgccgtgcg cgcccaattg atcggcgacc gcggacacgc ctttgccctt
     4921 gacagtgcgc gcgacgatcg ccgtcggtcg tccggtcgtg gcgactgcct cggcgtaggc
     4981 ccggtcgacg gcgtcgacgt cgtggccgtc gacctcgatc gcatgccagc cgaacgagcg
     5041 caggcggtcg gagtagacat cgaggtccca gccgtagcgt gtcggccccg tctggccgag
     5101 gcggttgaca tcgatgatcg ccgtgaggtt gtcgaggttg tcgaacgacg cctgcgccag
     5161 cgcctcccaa atcgaaccct cggccatctc cgagtcgcca cacaggaccc aggtccgata
     5221 tggcagcttg tcgagcttct ttccggcgag agcgattccg accccgattg gcagcccttg
     5281 gccgagcgag ccggtggcca cgtcgacgta cgggaggatc ggcgtcgggt ggccttcgag
     5341 gcggctgccg cgcatgcggt acgtcatgag ttcctgctca tcgacggcgc cgatcccctt
     5401 gaggatggcg tagaacaagg gcgaggcgtg gcccttggag aagatcaggt ggtcgttgtg
     5461 cggcgctcgg ggattcgcaa cgtcatagcg ccagtacttc gcgacgagca cggccatcag
     5521 ctcggccgcg gacatcgacg aggtgggatg cccggacttg gcggcgcttg aaacgcggat
     5581 ggaatcgatc cgtagctggc gcgccagatc aatccaggtc tgccgatcgg ggagggcgtc
     5641 ggtcatg
//
LOCUS       DATLCO010000239         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4100025, whole genome shotgun sequence.
ACCESSION   DATLCO010000239 DATLCO010000000
VERSION     DATLCO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4100025"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1370)
                     /locus_tag="VMZ33_05690"
     CDS             complement(156..1370)
                     /locus_tag="VMZ33_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16756.1"
                     /translation="MDPIARHYVVLSLALGEHIEGFVDAYYGPAELKQQAIASGAALV
                     SLAEQASALRERVANEEPDEQRRRWLDRQLVAIETLARRANGEETTYLDEVERCFDAR
                     PERTPAGEYANMRNDLERLLPGTGSLAERLKQRDQRLTIPRERVESVITWVVDQARAA
                     ASEIWPVPAGESLAIELVTDQPWSAYNWYDGDLRSRIEVNTDLPTRAMALPYLATHET
                     FPGHHLEHAWKEQRLVREHGRLEASVQLINTPEAYISEGLAEVGGNLLFDPAVWQDLL
                     IGICERSGIRMDAEDAEREWQTIEAMRALKRSSGDAALQLHVEGRSQEEVVQFLIDEA
                     LRTREQAEKSMEFLTHPLWRTYVFCYAGGEELLTTWCRAAGDAQAQRDRFFRLLTEQI
                     TPSGVAEELAAT"
     gene            complement(1376..2197)
                     /locus_tag="VMZ33_05695"
     CDS             complement(1376..2197)
                     /locus_tag="VMZ33_05695"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HUR16757.1"
                     /translation="MRQRQEVQALSRGLTLTDLPPERILDWEGCFNVRDLGGLPAADG
                     SRLKWGALVRSDIPTRLTQAGREALVAHGITSIVDVRFAEELAEDDDRYPFLDATDGA
                     PRRHHVPFHFSADDGPDAARHAAYAAAETRADLIRLDLDLNQSGITAAAAAIVDAQPG
                     GVMVHCHAGKDRTGIIVGVILAFLGVPDDEIADDYALTQRTFAPLVEEWLDSETDDAA
                     ERERLVALAWPSREAMLSALEHLRARYRSAADYLRDGGMTDEQLDRLRSRLLEET"
     gene            complement(2155..>2719)
                     /locus_tag="VMZ33_05700"
     CDS             complement(2155..>2719)
                     /locus_tag="VMZ33_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HUR16758.1"
                     /translation="MRRGIGLRGYAGIDPLNEFRREAFKLYEELRGFISKQVANTIFR
                     VQAQAARQSFPMPIPAQFSGQTSADGDGSRMDTITPVEGGHVHTDGTFHADNPAQPTP
                     AAPAAPAPVAAGAAKGASSSAAAAAVLPGLAAQPRRGIQYHHGDEPASGNGAMGGGQA
                     SPSGVKVGRNDPCWCGSGKKFKRCHGA"
BASE COUNT          415 a          892 c          933 g          479 t
ORIGIN      
        1 ccaaactcct attcctcccg ggctcaccct cgaccccgga aacgtgcgta aacacattca
       61 ccggaggagg cacggccccc ggaggcgggt gctaccgagt atggcaggag aagcgctcac
      121 gctcaagagg gcaaatttgc aagtgtgggt cgcgtttagg ttgcggcgag ttcctcggcg
      181 acgccggacg gcgtgatctg ttcggtcagc aagcgaaaga agcggtcgcg ctgcgcctgc
      241 gcatccccgg cagctctgca ccaggtcgtc aggagctctt cgccgccggc gtagcagaag
      301 acgtaggtac gccacagcgg gtgcgttagg aactccatcg acttctcggc ctgctcgcgc
      361 gtgcgcagcg cctcgtcgat gaggaactgg acaacttcct cctgcgacct gccctcgacg
      421 tgcagctgga gcgcggcgtc gccgctgctc cgtttgagcg cgcgcatcgc ttcgatggtc
      481 tgccactcac gctccgcgtc ctcggcgtcc atacggatac cgctgcgctc gcagatgccg
      541 atcaggaggt cctgccatac ggcggggtca aagagcaggt tcccgccaac ctcggcgagg
      601 ccctcgctga tgtacgcctc gggcgtgttg atgagctgga ctgacgcctc cagccggccg
      661 tgctcgcgaa caagccgctg ctctttccag gcatgctcga ggtgatgacc ggggaacgtc
      721 tcatgagtcg ccaggtatgg cagcgccatt gcacgcgtgg ggaggtcggt gttgacctcg
      781 atgcgtgatc ggagatcgcc gtcgtaccag ttgtaggccg accacggctg gtccgtgacg
      841 agctctatgg cgagcgactc gcccgctggg accggccaga tctctgacgc tgccgcccgg
      901 gcctggtcga cgacccatgt gatcaccgat tcgacgcgtt ccctggggat ggtcagccgt
      961 tggtcgcgct gcttgagtcg ctccgcgagc gaaccggtgc cgggcagcaa gcgctccaga
     1021 tcattccgca tgttcgcgta ctcgccagcc ggagtccgtt ccgggcgcgc gtcgaagcac
     1081 ctctcgacct cgtcgaggta ggtcgtctcc tcgccgttcg cccgtcgtgc cagggtctcg
     1141 atggctacga gctgtcgatc gagccagcgc cgacgctgtt cgtcgggctc ctcgtttgcc
     1201 acccgttcgc gcagtgcgct cgcctgttcg gccagcgaaa cgagtgccgc gccggaggcg
     1261 attgcctgct gcttgagctc ggccgggcca tagtaagcgt cgacaaagcc ctcgatgtgc
     1321 tcacccagcg ccagcgagag gactacgtag tgccgcgcaa tcgggtccat gggcgtcatg
     1381 tctcctccag taggcggcta cgtaagcggt cgagctgctc gtcggtcatg ccgccgtcgc
     1441 gtaggtagtc agcggctgac cgatagcgcg cgcgcagatg ctccagtgcg gaaagcatcg
     1501 cctcgcgcga cggccaggca agcgccacga gtcgctcacg ctccgcggca tcgtccgttt
     1561 cgctgtccag ccactcctca acgagcggcg cgaaggtgcg ctgggtcagc gcgtagtcgt
     1621 cggcgatctc gtcatcgggc actccaagga acgccaagat cacgccaacg atgatgcccg
     1681 tccgatcctt gccagcgtgg cagtgaacca ttacgccgcc tggctgcgca tcgacgatcg
     1741 ccgcggcggc ggccgtgatg ccggattggt tgaggtccag atcgaggcgg atcaggtcgg
     1801 cacgcgtctc ggcggccgcg taggcggcgt ggcgcgcggc atcgggtccg tcgtcggccg
     1861 agaagtggaa tggcacgtgg tgtcggcgtg gggcgccgtc ggtcgcatcg aggaaggggt
     1921 aacggtcgtc gtcctcggcg agctcctcgg cgaagcgcac gtcgacgatc gacgtaatcc
     1981 catgggcgac aagcgcctcg cgcccagcct gggtcagacg agtcgggatg tccgagcgga
     2041 cgagggcgcc ccatttgagg cggctgccgt cagctgcggg caaaccgccc agatcgcgga
     2101 cgttgaagca accttcccag tcaagaatgc gctccggggg gaggtcggtg agcgtcaagc
     2161 cccgtgacag cgcttgaact tcttgccgct gccgcaccag cagggatcgt tacggccgac
     2221 cttcacgccc gacggactgg cctgaccgcc acccatggcg ccgttgcctg atgcgggctc
     2281 gtcgccgtgg tggtactgga tgccgcgccg tggctgcgcc gccagaccgg gcagcacggc
     2341 cgctgccgcg gcgctgctac ttgcaccctt ggccgctccc gcggcgactg gggctggggc
     2401 ggctggggcg gctggggtcg gctgggcggg attgtcggcg tggaatgtcc cgtcggtgtg
     2461 gacgtgtccg ccctccaccg gcgtgatcgt gtccatccgt gagccgtcgc cgtcggcact
     2521 ggtctggcct gagaattgcg ccggaatcgg catcggaaac gactggcggg ccgcctgtgc
     2581 ctggacgcgg aagatcgtgt tggcgacctg cttgctgatg aagccgcgca gctcctcgta
     2641 gagcttgaag gcctcacgtc ggaactcgtt caggggatcg ataccggcgt agccacgcag
     2701 tccgatgccg cgtcgcatg
//
LOCUS       DATLCO010000240         2296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1885776, whole genome shotgun sequence.
ACCESSION   DATLCO010000240 DATLCO010000000
VERSION     DATLCO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885776"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VMZ33_05705"
     CDS             <1..316
                     /locus_tag="VMZ33_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013177.4"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HUR16759.1"
                     /translation="PLYRISTGKVTRYAKDDADRERIIKEFKSKNLSIQRFKGLGEMN
                     ADQLWETTMSPATRTLLRADVDEEGAGETDRIFSALMGEKVEPRRDFIKARARQVQNL
                     DV"
     gene            341..1315
                     /locus_tag="VMZ33_05710"
     CDS             341..1315
                     /locus_tag="VMZ33_05710"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HUR16760.1"
                     /translation="MTERAQTFVTERLPEARGLGIALVELLDDPDAFTDALREGLGRL
                     SDPEYRHEQERVAPGSGEVFGVRNPLMAAIARQIREPLRRISSTWALSLAERLIAEDE
                     REIVLFSHVALRRALPNDPEQSWQLMRRLARRAHDWISVDELAELYAYGILLEQFRWA
                     ELEQLVFSLDKWERRLVGSTIARLPFELPKHQRSALAATSGLTLIKSLLGDADPDVQK
                     ALSWALRSWTEVDSPAVEAFLRVEAVNARSTDDGHSAWVLRDALTWPGTPLALQNAVR
                     ATLQGVRRRPNAPSTSEASSVAGNFIGLDRMAEAAVAMQGDRQRMGRG"
     gene            1319..>2296
                     /locus_tag="VMZ33_05715"
     CDS             1319..>2296
                     /locus_tag="VMZ33_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HUR16761.1"
                     /translation="MAEETGHIIPIRIEDELKVSYLDYAMSVIVSRALPDVRDGLKPV
                     HRRILYTMHEMGLTSGASYRKCAAIVGEVMGKYHPHGDVALYDALVRLAQTFSLRYPL
                     IDGQGNFGSVDGDPPAAMRYTEARMTAIAAEVLADIDKQTVDFVENYDGTRMQPSVLP
                     AKLPNLLVNGSAGIAVGMATNIPPHHLGEVVDATIALIDDPNLTNDELCNIVTGPDFP
                     TGGTIFRFDRRRNSITGQWEEVDAIRDMYAHGRGRIVMQAVVSFEETRQQRTAIVVTE
                     LPFQVNKASLAEKIADLVQGKKIEGIADIRDESDRDGMRLVIECKRDAAP"
BASE COUNT          435 a          767 c          727 g          367 t
ORIGIN      
        1 gccgctctac cggatcagca ccggcaaggt gactcgctac gcgaaggacg acgccgaccg
       61 cgagcggatc atcaaagagt tcaagtccaa aaacctctcc atccaacgct tcaagggcct
      121 cggtgagatg aacgccgacc aactctggga gaccaccatg agcccggcga ctcgaacgct
      181 tctgcgggca gatgtcgacg aggagggcgc cggcgagacc gaccgcatat tcagcgcact
      241 catgggtgag aaggtcgaac cccggcgcga cttcatcaag gcccgggccc gccaggtcca
      301 aaacctcgac gtctaggcgc ggttgaattt ggcttcacca gtgaccgagc gtgcccagac
      361 gttcgtgacg gagcgcctgc ccgaagcacg cggcctcggc atcgccctgg tcgagctgct
      421 cgacgatccc gacgcattca ccgacgcgct ccgcgaggga ctcgggcgac tctcagatcc
      481 cgaatatagg catgagcagg agcgagtggc tccggggtcg ggcgaggtgt tcggagtccg
      541 gaacccgctc atggccgcca tcgcgcgcca gatccgggag cctctccgcc gcatctcgtc
      601 cacctgggcg ctctcgctcg cggagcggct gatcgccgag gacgagcgcg agatcgtgct
      661 cttcagccat gttgctctcc ggcgcgcact tcccaacgat cccgagcagt cgtggcagct
      721 catgcgccgc ctggcacgcc gcgcccacga ctggatcagc gtggacgagc tcgccgagct
      781 ctacgcatac ggcatcctcc tggagcagtt tcgctgggcg gagctcgagc agctcgtgtt
      841 ctccctcgac aagtgggagc ggcggctggt cggctcaacc atcgcgcgac ttccgttcga
      901 gctgccaaag caccaacgct cggcattggc cgcgacgtcc ggtttgacgc tcatcaaatc
      961 gttgctcggc gatgcggacc cggatgtaca gaaggcgctc tcatgggcgc tacgcagctg
     1021 gaccgaggtg gattctcccg ccgtcgaggc attcctaagg gtcgaggccg tcaacgcacg
     1081 ttcaaccgac gatggccaca gcgcatgggt cctcagagat gccctgacgt ggcctgggac
     1141 accattagcg cttcagaacg ccgtacgggc cacgctacag ggcgtgcggc gccggccaaa
     1201 cgccccatcg acgagcgagg cgtcgtcggt cgcaggcaac ttcatcgggc tcgatcgcat
     1261 ggccgaggcg gccgtcgcga tgcagggcga tcgccagagg atggggagag gctgattagt
     1321 ggctgaagaa accgggcaca taatccccat tcgtatcgag gacgagctga aggtcagcta
     1381 cctcgactac gcgatgagcg tgatcgtcag tcgtgctctg cccgacgttc gcgatggcct
     1441 caagcccgtt caccgccgga ttctgtacac gatgcacgag atgggactca cctccggggc
     1501 gagctaccgg aagtgcgccg cgatcgtcgg cgaggtgatg ggcaagtacc acccgcacgg
     1561 tgacgtcgcc ctgtacgacg cacttgtccg cctcgcccag accttctcac tgcgttatcc
     1621 gctcatcgat ggtcagggca acttcgggtc cgtcgacggc gatccaccgg ccgccatgcg
     1681 gtacacggaa gcacgcatga ccgcgatcgc ggctgaggtc cttgccgaca tcgacaagca
     1741 gaccgtggat ttcgtcgaga actacgacgg cacgcgcatg cagccctcgg tgctgccggc
     1801 caagctcccc aacctgctcg tcaacggttc ggcggggatt gccgtgggca tggcgaccaa
     1861 catcccgccg caccacctgg gggaggtggt cgacgcgacc atcgccctta tcgacgaccc
     1921 caacctaacc aacgacgagc tgtgcaacat cgtcaccggt ccggacttcc cgaccggcgg
     1981 caccatcttc cgcttcgacc gtcgtcgaaa ctcgatcacc ggtcagtggg aagaggtcga
     2041 cgcgatccgc gacatgtatg cccacgggcg cggccgcatc gtgatgcagg ccgtggtcag
     2101 tttcgaggag acgcggcagc agcggactgc gatcgtcgtc accgagctgc cgtttcaggt
     2161 gaacaaggcg agtctcgccg agaagatcgc agacctcgtc cagggcaaga agatcgaggg
     2221 catcgccgac atccgcgacg agtccgaccg cgatggcatg cggctggtca tcgagtgcaa
     2281 gcgcgacgcc gccccg
//
LOCUS       DATLCO010000241         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4110849, whole genome shotgun sequence.
ACCESSION   DATLCO010000241 DATLCO010000000
VERSION     DATLCO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4110849"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VMZ33_05720"
     CDS             <1..808
                     /locus_tag="VMZ33_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HUR16762.1"
                     /translation="AEPKPEIVEAVGAYLDVASLLGTRTAQLHNALATARDDVSFVPE
                     PFSALSQRSVFQTMRNNAKATIALLNEVRDELPEPAQQLAERAAELGPTILQRMSDIT
                     RDKIAASRIRVHGDFHAGQILWTGRDFVFIDFEGEPGRPLSERRHKRSAMVDVAGMLR
                     SFHYAAFGTLLSPRVGGTVRAEDLPQLEPWANLWYLSVASAFLRAYLNEADGQPFVPT
                     DRKELVHLLEASALQKVLYELSYELNNRPDWVTIPLRGLVDLIGEARTSG"
     gene            1018..2208
                     /locus_tag="VMZ33_05725"
     CDS             1018..2208
                     /locus_tag="VMZ33_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR16763.1"
                     /translation="MSTALSGPLGTLSLAVALVAATAAVKLSLYLIAVGNTGYVIYIP
                     AVAFAAWYRGLGAGAFATLLAVAMDMLVFEFLTPGVPGLPDQEIRLLAFVAASLLLTY
                     FSYRLRTDRDAARNDADKRSQALTEAAQARQEVDRVVSHERRANELRDAFNSIVSHEL
                     RTPITSIYGGAKLLASRERVLDDHTRRELIDDLEVEADRLYRLVEDLLVLARSERGTV
                     ERVAEPILLSRMVARVVRSEHERWPASKFVVTAKGSESTARGDETYVEQVIRNLVSNA
                     AKYSPAGSVVRVIVDETSEGIRLRVLDEGAGIDANEAPRLFDLYYRSPHTAHTVSGAG
                     IGLYVCRVLIAAMGGRVWALPRPDGGAEFGFVLQRAEEDPVSGEIVASPTKLRTVATA
                     ARAG"
     gene            complement(2174..>2582)
                     /locus_tag="VMZ33_05730"
     CDS             complement(2174..>2582)
                     /locus_tag="VMZ33_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HUR16764.1"
                     /translation="PESDVVTMVVERDPRLRTMAVFDALANNADRKGGHILPMTDGHV
                     YGVDHGICFAVEPKLRTVLWGWRGDALSDEEVGVVRGVCEALDDGPLEAELRALLTAE
                     EVRATRRRADALLSSGRFPEPDPSRPALPWPPF"
BASE COUNT          433 a          872 c          830 g          447 t
ORIGIN      
        1 cgcggagccg aaaccggaga tagtggaggc cgttggcgcg tatctggacg tcgcctcact
       61 gcttggcacg cggacggcgc agctccacaa cgccctcgcc actgcccgcg acgacgtctc
      121 tttcgtgccc gagccgttca gcgcgctctc tcaacgctcc gtcttccaga cgatgcgcaa
      181 caacgccaag gcgacgatcg cgctcctcaa cgaggtacga gacgagctgc cggaaccggc
      241 tcaacagctg gccgaacgag ccgcagagct gggcccgacg atcctccagc ggatgtccga
      301 tatcacccgc gacaagatcg ctgccagccg gatacgggtt catggcgact ttcatgccgg
      361 ccagatcctg tggacgggtc gcgacttcgt cttcatcgac ttcgaaggcg agcccggccg
      421 gccactctcc gagcgccggc acaagaggtc ggcgatggtc gacgtggccg gcatgttgcg
      481 gtcgttccac tacgcggcat tcggaacgct gctgagcccg cgtgtcggcg gcaccgttcg
      541 tgccgaggac ctgccgcagc tcgagccgtg ggcaaacctc tggtacctga gcgtcgccag
      601 cgcattcctg cgcgcctacc tgaacgaggc tgacgggcag ccgttcgtgc ccaccgatcg
      661 caaggagctt gtccatctgc tggaggcgtc ggcgctccag aaggtgctct atgagctcag
      721 ctacgagctc aacaatcggc cggattgggt caccatcccg ctgcgcgggc ttgtcgatct
      781 gatcggtgag gcgcgcacct cagggtaggt cggacgttca cgcattcgtc acgacttcgg
      841 cggtgttggc gctcgccttc gccatcagcg atgggccaat ctcttggcag cccgccgtca
      901 accacttgaa cactcaaccg gtcggtttgg agatgaatgg agcacccgac ggcatcaccg
      961 gccgatcaga tgacggctgg gtcacgcccg acgccgggac gtgttggcat ggctcaattg
     1021 tcgaccgcgt tgtcagggcc tctgggcacg ctcagcttgg ccgtcgcgct tgtggccgcg
     1081 acagccgccg tgaagctgtc cctctacctg atcgccgtcg gtaacaccgg atatgtgatc
     1141 tacataccgg cggtcgcgtt cgctgcgtgg taccgcgggt tgggcgccgg cgcgttcgcg
     1201 accctgctcg cggttgccat ggacatgctg gtttttgagt tcctgacacc tggagtgccg
     1261 gggcttccgg accaggaaat tcgcctgctt gcgttcgtcg ccgccagcct gctcctaacg
     1321 tacttctcgt accggttgcg cacggatagg gacgccgctc gcaacgatgc cgacaagcgc
     1381 agccaggccc tgacggaggc cgctcaagcc aggcaagagg ttgaccgcgt cgtctcccat
     1441 gaacggcgcg cgaacgagct gcgtgatgcc ttcaacagca tcgtttcgca cgagctgcgc
     1501 acaccgatca cgtcgatcta cggcggagcg aagctgctcg ccagccgcga gcgtgttctc
     1561 gatgaccaca cccgtcggga gttgatcgac gacctggagg ttgaagccga ccgtctctat
     1621 cggctggttg aggacctcct ggtgctggcg cgatcagagc gcggcaccgt cgagcgcgtg
     1681 gccgagccca tcctcctgtc gcggatggtc gcgcgcgtcg tgcgcagcga gcacgagcgc
     1741 tggcccgcct ccaagttcgt ggtaactgca aaaggatccg aatcgacggc ccgtggcgac
     1801 gaaacgtacg tcgaacaggt catccgcaac ctcgtcagca acgcggccaa gtacagtccc
     1861 gcgggctcgg tggtccgggt gatagtcgac gaaacctcag aggggatccg cctgcgggtt
     1921 ctcgacgagg gcgcaggtat cgatgccaac gaggcgcccc gtctgttcga tctgtactac
     1981 cgctcacccc atacggcaca cacggtcagc ggcgcgggca tcgggctcta cgtgtgccgc
     2041 gtcctcatcg ccgccatggg cggccgggtc tgggccctgc cacgtcccga cgggggtgcc
     2101 gagttcgggt tcgtcctgca gcgtgccgag gaagaccccg taagcggcga gatagtcgcg
     2161 tcgccgacaa agctcagaac ggtggccacg gcagcgcggg ccggctaggg tccggttcgg
     2221 gaaagcgtcc cgaagacagg agagcgtcgg cccgacgcct ggttgcgcga acctcctcgg
     2281 cagtcagcag cgccctcagc tcggcttcaa gcggaccgtc atccagtgcc tcgcacacgc
     2341 cacgcacgac ccccacttcc tcgtcgctca gcgcgtcgcc gcgccagccc cacaacaccg
     2401 ttctgagctt gggctcgaca gcaaagcaga tcccgtggtc gacgccatac acgtggccgt
     2461 cggtcatcgg caggatgtgg ccacccttgc ggtcagcgtt gttggccagc gcgtcgaaga
     2521 ccgccatggt tcgcagtcgt gggtcgcgct cgacgaccat cgtcaccacg tccgactcgg
     2581 gg
//
LOCUS       DATLCO010000242         2809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1894762, whole genome shotgun sequence.
ACCESSION   DATLCO010000242 DATLCO010000000
VERSION     DATLCO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894762"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VMZ33_05735"
     CDS             complement(<1..195)
                     /locus_tag="VMZ33_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16765.1"
                     /translation="MYFYELSEADDEIFANVLLAHDSEYDETEYFDLVMEAREAVLRN
                     FEEESLIEAIATELERKHGFV"
     gene            237..1142
                     /locus_tag="VMZ33_05740"
     CDS             237..1142
                     /locus_tag="VMZ33_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HUR16766.1"
                     /translation="MPDTPTVVVVGAASRDIDAADPRGWRLGGTVTYSALAAAEVGVR
                     VRALIGVDQEAAAAHELDVLRDAGVEIHLVPLSRGPVFHNQPLPDGGRAQVAVSISES
                     IPMSALPDAWRHPSAALLGPIAAELGDDWASAFSVPTRTALAWQGMLRSLDPGQPVRP
                     LPPTRTSLIASADALFISAEDVSGDMTPIHDLLGAGQQLVVTHGGYGAVHLSLGEHSK
                     LDGRYLPPTPRRKPIDTIGAGDTFLGAWLAATALLAPATDPWRALLVASVTASLSVVR
                     VNLRDHPTRADICEELVRLRDSRLG"
     gene            complement(1114..2415)
                     /locus_tag="VMZ33_05745"
     CDS             complement(1114..2415)
                     /locus_tag="VMZ33_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HUR16767.1"
                     /translation="MDISIASGRPAASGLLSRRLDRLRSSTVPAPSQARASTPDFSAA
                     LAQELGGQVERSAGGSIAVFESTFAGLGLDRARLAGLPYPVPDGHPLVCIDLETTGLA
                     TAAGTLAFLVGVGTWVGESMVVRQLVLPDHAGETALLDVLCSVVPADAWLVTYNGRCF
                     DWPLLVARYRLHRRAAPVHAGHLDLLPVARQLWKHRLEGARLAIVEREICGVMRDDDL
                     PGHLIPERYFTYLRSRRAELLRPILEHNRQDIVSLGQMLGRLVAGFGSPEAWPATHPG
                     DVFGLARAFARQGRHPEALNAVEAALGADAWSLGVTGGAGLHRRLSAERARLLARVGR
                     RDEAYRAWSEIALRGGPGAGLAWLAVARYREHTLRDIAGALDACQQAASVAERARAWG
                     NPLFVVERDLARRLPRLRRVAFRRRPFRPTNAANRDVSRAA"
     gene            complement(2455..>2809)
                     /locus_tag="VMZ33_05750"
     CDS             complement(2455..>2809)
                     /locus_tag="VMZ33_05750"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HUR16768.1"
                     /translation="AGAGRALQTVASVLLMADPRDLGVVAQVRSPHQERPVVYLYDGV
                     PGGVGLAERLFDRHEELIAGALGLVESCACSEGCPACVGPRLEAGGMGKTLAQRLLQG
                     LAGPTDASAGLTEAA"
BASE COUNT          473 a         1032 c          915 g          389 t
ORIGIN      
        1 gacgaacccg tgtttgcgct cgagctcggt ggcgatcgcc tcgatgagcg actcctcctc
       61 gaagttgcgc aagacggcct cgcgcgcttc catgacgagg tcgaagtatt cggtctcgtc
      121 atactcggaa tcgtgcgcaa ggagcacgtt cgcaaagatc tcgtcgtccg cctcggacag
      181 ttcgtagaag tacacgactc ctccccggat tgagcgtggt cgagccgagt ataggcgtgc
      241 ccgatacacc gaccgtcgtg gtcgtcggtg ccgccagccg agatatcgac gccgccgatc
      301 cgcgtggctg gcgactgggc ggaacggtta cttattccgc gctcgcggcg gccgaagtcg
      361 gcgtccgagt ccgtgccctg atcggagtcg atcaggaagc ggccgcggcc cacgagctcg
      421 acgtcctgcg cgacgccggg gtcgagatcc acctagtgcc gctatcacgt gggccggtat
      481 tccacaacca gccactcccc gatggcgggc gcgcgcaggt ggcagtcagc attagcgaaa
      541 gcatcccgat gtcggcgctg cccgacgcat ggcgtcaccc gtccgcagcg ctcctgggtc
      601 cgatcgcggc cgagcttggc gacgactggg cgagcgcctt tagcgtgccg acacgcaccg
      661 cgctggcgtg gcaggggatg ttgcgctcac tcgaccccgg ccagccggtt cgtcctctgc
      721 cgccgacgcg cacatcactg atcgcgtccg ccgacgcgct gttcatcagc gccgaggacg
      781 tcagcggcga catgaccccg attcacgacc tgctgggggc gggccagcaa ctcgtcgtca
      841 cgcacggcgg gtacggcgcc gtgcacctct cgttgggcga acacagcaaa ctcgacggtc
      901 gctacctccc gcccacgcca cggcgcaagc cgatcgacac catcggcgcc ggcgatacct
      961 tcctcggcgc ctggctggcc gcgacggccc tcttggctcc cgcgaccgat ccctggcggg
     1021 ctctcctcgt ggcttccgtc accgcgagcc tttccgtcgt gcgcgtcaac ctccgtgacc
     1081 acccgacccg agccgatatc tgcgaggagc tggtcaggct gcgcgactca cgtctcggtt
     1141 agccgcgttc gtcggtcgga agggccggcg acggaaggcc actcgacgca gccggggcaa
     1201 gcggcgcgct agatcgcgct ccaccacgaa aagcggattg ccccaggcac gggcgcgctc
     1261 ggccacggag gcagcctgct ggcaagcatc cagcgcgccg gcgatgtcgc gcagcgtgtg
     1321 ctcgcggtag cgcgccacag ccagccacgc caaacccgcg ccgggtccgc cccgcaacgc
     1381 tatctccgac catgccctgt acgcctcgtc acgtcgacca acccgtgcca gaaggcgcgc
     1441 ccgctcagca gacaggcggc gatgcagacc ggcgccaccg gtaacgccga gcgaccacgc
     1501 gtcggcgccg agtgccgcct ccacggcatt caacgcctcc ggatgccgtc cctgccgagc
     1561 gaaggcgcgg gccaacccga acacatcgcc cggatgtgtg gctggccacg cttcgggcga
     1621 tccgaacccc gcgaccagac ggcccagcat ctgacccagc gaaacgatgt cctgccggtt
     1681 gtgctcaagg atgggccgga gaagctcggc gcggcgggaa cgcaggtacg tgaagtagcg
     1741 ctccgggatc aggtgtccgg gcaggtcgtc gtcacgcatg acgccgcaga tctcccgctc
     1801 cacgatggcc agccgcgcgc cttcgaggcg gtgcttccac agctgccggg cgacgggcag
     1861 caagtcgagg tgaccggcat gcaccggcgc cgcgcggcga tggagccgat accgggccac
     1921 cagcagcggc cagtcgaagc agcgaccgtt gtaggtgacg agccaggcat ctgccggcac
     1981 aacggaacac aggacgtcga gcaaggctgt ctcaccggcg tgatccggga gcacgagctg
     2041 gcgcaccacc atgctctcgc ccacccatgt ccctacacca acgaggaacg ccagcgtccc
     2101 ggccgccgtg gccagcccgg tcgtctccag gtcgatgcac accagtgggt gaccgtcggg
     2161 aacggggtag gggaggccgg ccagccgcgc ccgatcgaga cccagaccag caaacgtgct
     2221 ctcgaagact gcgatcgagc cacccgccga ccgctcaacc tgtccaccga gctcctgcgc
     2281 cagcgccgcc gagaaatcag gcgtactcgc ccgtgcctgc gacggcgcag gcacggtgga
     2341 ggatctgagg cggtcgaggc gtcgcgatag aaggccgctc gccgcgggcc gtccgctcgc
     2401 gatagatatg tccatggcgg caaaccttgg gccgcgtccg tgccagctag gttgtcacgc
     2461 cgcctcagtc agccccgccg acgcgtcggt tggtccggcc agaccctgca gcagccgctg
     2521 cgccaaggtc ttgcccattc cgccagcctc cagccgcggg ccgacgcacg cgggacaacc
     2581 ctccgagcac gcgcatgact cgacgaggcc cagggcgcca gcgatcagct cctcgtgccg
     2641 atcgaacaac cgctcggcca gcccgacacc ccccggcacg ccgtcgtaga ggtatacgac
     2701 gggtcgttcc tggtgtggcg agcgcacctg cgccacgacg ccgagatcgc gcggatcagc
     2761 catcagcagc accgaagcga ccgtctgaag cgcccgaccg gcaccggcc
//
LOCUS       DATLCO010000243         4944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8404255, whole genome shotgun sequence.
ACCESSION   DATLCO010000243 DATLCO010000000
VERSION     DATLCO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8404255"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1306
                     /locus_tag="VMZ33_05755"
     CDS             122..1306
                     /locus_tag="VMZ33_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HUR16769.1"
                     /translation="MHAYPSCVTKLQVVVVAAALAVVGCGSPASTGPASTDQTGAPSS
                     ATPFAIATASQLALSSPTATPTPTATTVVVATDGLQSPLPSPQPVGWREFSDGGPSGR
                     GDHTWTVDGNGATAYLFGGQTADGASNELWAFDLATDTWTVVEASGAPPAPRFGHTAT
                     WVPDVGLVVWSGQGRGAEFFDDIWAYDPGVNAWLQLPSFGSVPDARYGSCAALGPDGE
                     LWISHGFTFSGRFADTRSYNFDDGSWTDRTPLGNAPVERCLHDCYWSADDRLVLYAGQ
                     TTGVAALRDIWAYEYDLGEWTRGPDPEAAARQLYGLATDSTGTFAFVYGGGSLDGGYL
                     DDAWSIDSGALELGPVEAPQGPQARSGAALILHSIRDRLLLFGGTSADGPLSDTWELG
                     LI"
     gene            complement(1520..2407)
                     /locus_tag="VMZ33_05760"
     CDS             complement(1520..2407)
                     /locus_tag="VMZ33_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16770.1"
                     /translation="MSDIALVTCSRLPELDPDDRPLIEHLGRRGHSAVPATWDDLEVD
                     WKRFAVVVLRNPWDYTDRRDEFVAWAKSVASLLNPADVVEWNTDKRYLGDLARAGVPI
                     VPTTWIEPGDVVRLPDEGRHVLKPSVGAGSLDAAAFSLHDIREQQLARDHAQRLLATS
                     QTVMLQPYLDLIEERGETGLIYMGGEFSHAIGKGPMLVRQTKVEGDALYKEEVITARE
                     ALADELEVGRQALEAVPGGSARLLYARVDMVPGPDGSPLLMELELTEPSLFMSTTPGT
                     VERFADAIAARALVGSRQT"
     gene            complement(2442..>4944)
                     /locus_tag="VMZ33_05765"
     CDS             complement(2442..>4944)
                     /locus_tag="VMZ33_05765"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HUR16771.1"
                     /translation="RLHSRRTQQAANEGVRGIGMYTDSGTVNAISTSSSRRIRLSAAA
                     FLLALTTALPMTVAHAAQPTASPSPSPTPTVQPSPEGTTKPKPKPSPTATPAPDPTPT
                     PRATAQPELSEYAERVEAVIAAARQYIGHPYRVGSEGPVFIDCSGLVYRAFTDANEGN
                     QIGVWRMRAAGYQRWFAGRELLTSDLEDVERGDLVVYNGGTHIGIYLGDGRVISALTS
                     GVTVHSLNGITLPVTAYLAVDWNGKRGPFVPGVIPAPIDAPEEPASLVPPLAWSPEIT
                     DETLVAGPAIDGEERVDLRTANSRTWETDEGKFTTEFFGRPIHYLPADSTDWQPIDLR
                     FSVPEDEDAGVAVASAAPVSLALREADSEDGLLTLSGSNAAGEELNVSLGLPHVGRNA
                     EEPVLALDGSYADYRNALGRDRALRILPRADGFKTYLILPDRPASNEVAFAVEAGTLT
                     LTPEPDGSVTLRDADGSIYGRVTRPMLLDSSDVEGDGGGVRASAVTLTVVPAADEESP
                     DVLTLTIDRDLLAEAVYPAFVDLTLVDFPSNAAAAQHTFASSAHPDSNFSVFQRPEAP
                     GYAELWHGRRPGRDDNNEIYLRFGGLADALRGATVESAGLAAFPYWQNAGDDPRSTWL
                     APITAEWDLRTLSWNTRPLPATEASDYETTQGQWTAMDLTAYTQSIVDGSALDYGLVL
                     HASQQGRGYWKRFVGESTLGGGALEPRLTVAWSAPRPSSGQATFDETGTQVSLAWSHG
                     TLVPDADRVQVQVSADGFATILTDTKLKGRHAGDLTLAIPTGDLEAATYSFRVRARYG
                     EGAAWSSWSDAGTFTIAAPVPPAHLMDDSRFRQAV"
BASE COUNT          794 a         1591 c         1703 g          856 t
ORIGIN      
        1 gccgatgatg acgccgatcg ccagcgcgag cgcgttcgtc ttcagcagaa acgccctgaa
       61 ttcgctcatc agcggtgagc ctcctcattc atccgacacc ccccgaccgg gcgaacgata
      121 gatgcatgcg tacccttcct gcgtgaccaa gttgcaggtg gttgttgtgg ctgcggctct
      181 cgccgttgtc gggtgtggca gcccggcttc aacggggccg gcttcgacgg atcagactgg
      241 cgcgcccagc tcggcaacgc ccttcgcgat agcgaccgcc agtcaattgg cattgtccag
      301 cccaacagca acaccgacac cgacggcgac gaccgtcgtt gttgccaccg acggtctgca
      361 gtcgcccctt ccgtctccgc aaccggtcgg ttggcgggag ttttccgatg gtggaccgag
      421 tggtcgcgga gaccacacct ggacagtaga cggcaacgga gcgacggcat acctcttcgg
      481 cggacaaacc gcggacgggg cctcgaatga gctatgggct ttcgacctag ccaccgacac
      541 ttggaccgtc gttgaggcga gcggtgcacc ccctgcgccg aggttcggcc acaccgcgac
      601 atgggtcccg gacgtggggc tcgtcgtatg gagcggacag ggccgagggg ccgaattctt
      661 cgacgacatc tgggcatacg atcctggggt caacgcctgg ctgcagctac cgtcattcgg
      721 cagcgtgccg gacgcgcggt atggatcgtg cgcagcgttg gggccagatg gtgagctctg
      781 gataagccac ggcttcacct tcagcggtcg ttttgcggac acgcgttcct acaactttga
      841 cgatgggtca tggacggatc ggacgccgct cggtaacgcg ccggtggaac gctgtctcca
      901 cgactgctac tggtcggccg acgaccggct cgtgctctac gcgggtcaga ccactggggt
      961 ggccgcgttg agggacatct gggcatatga atacgacctc ggggagtgga cgcgtggccc
     1021 ggatccggag gccgcggccc gccagctgta cggtctcgct accgattcga ctggaacctt
     1081 cgcattcgtc tatggcggcg gctcattgga cggtggctat ctggacgacg cttggtcgat
     1141 agactccggg gcgctcgagc tcgggccggt ggaggcgccc cagggtccgc aggcgagatc
     1201 cggcgcggcg ctcatcctcc attcgatacg ggaccgtctg ctgttgttcg gcggcacgag
     1261 cgccgatggg cccttgagcg atacctggga gctcggactc atctagcgct gggtccacgt
     1321 ccgtacatcg gtacgtgcct gtacttagaa cactgcccgt ggccgattcc gtggcaaacc
     1381 ggatttaccc gggaaccgct cgggcataac ttgcgtccag acgttgacgc ggagaaccct
     1441 ctcctccgcg gaacggtcag gagacctctc tcctgctgag gccgggcttg ctgcgctggg
     1501 gcgcgcgccc ggcctccttc tatgtctggc ggctgccgac gagggcgcgc gcggcgatgg
     1561 catcggcgaa acgctcgacc gtaccggggg tggtcgacat gaacaacgac ggctcggtga
     1621 gctcgagctc catcaataac ggtgagccat cagggccagg caccatgtcg acgcgcgcat
     1681 agagcagccg agcgcttccg cccgggacgg cttcgagagc ctgccggccg acctcgagct
     1741 cgtccgccaa cgcctcacgc gcggtgatga cctcttcctt gtagagggca tcgccttcga
     1801 ccttcgtctg acggacgagc atcggcccct tgccgatcgc gtggctgaac tccccaccca
     1861 tgtagatgag cccggtctcg ccccgctcct cgatgaggtc gaggtatggc tggagcatca
     1921 cggtctgact cgtggccagg agccgctggg cgtgatcgcg ggccagctgc tgctcgcgga
     1981 tgtcgtgcag gctgaaggct gctgcgtcca gcgagccggc gcccacggac ggcttgagca
     2041 catgccggcc ctcgtcgggg agcctcacga cgtcaccggg ctcgatccat gttgtcggga
     2101 cgatgggcac acccgcccgc gcgaggtcgc ccagatagcg cttatcagtg ttccactcga
     2161 cgacgtcagc cgggttcagc agactcgcca cggatttggc ccaggccacg aactcgtcgc
     2221 gccggtcggt gtaatcccac gggttgcgga gcacgacgac ggcaaaccgt ttccagtcga
     2281 cctcgaggtc gtcccaagtg gcaggtactg cgctgtgccc gcgacgtccg agatgttcga
     2341 tcaggggtcg gtcgtcggga tcgagctccg gcagccgaga acacgtaacc agggcgatgt
     2401 ccgacatgcc tgcgattctc gcggccattg gggatcgagg tctagacggc ctggcggaac
     2461 ctcgagtcgt ccatgaggtg tgccggtggg acgggtgcgg cgatcgtgaa tgttcccgcg
     2521 tccgaccagc tgctccaggc agcgccctca ccgtaacggg cacgaacgcg gaacgagtac
     2581 gtcgcggctt cgaggtcccc ggtcgggatg gccagtgtca agtcacccgc gtgccggccc
     2641 ttcagctttg tatctgtgag gatcgtggcg aagccatcgg ccgagacctg gacctgtact
     2701 cggtctgcgt caggaacgag cgtcccgtgc gaccacgcca ggctcacctg tgtgcccgtc
     2761 tcgtcgaaag ttgcctgtcc cgatgagggc cgtggcgctg accaagcgac cgtcagtcgc
     2821 ggctcgagtg cgccaccgcc gagagtgctc tcgcccacaa aacgcttcca gtagccgcgg
     2881 ccctgctggc tcgcgtggag taccagcccg taatcaaggg ctgagccatc gacgatcgac
     2941 tgcgtgtagg cagtgagatc cattgccgtc cattggccct gagtcgtctc gtagtccgat
     3001 gcctcggtcg cgggcaatgg gcgcgtgttc cagctcagtg tgcgcaggtc ccactcagcg
     3061 gtgatcggcg cgagccacgt cgaccgcggg tcatcgccgg cgttctgcca gtaggggaac
     3121 gctgccaggc cggccgactc gaccgtcgcg ccgcgcagcg cgtcagccag accaccgaag
     3181 cgcaggtaga tctcgttgtt gtcgtcccga ccgggccggc ggccgtgcca cagctccgcg
     3241 taacccggcg cctctggacg ctggaagacg ctgaaattgg agtcgggatg tgcgcttgac
     3301 gcaaacgtgt gctgcgccgc agctgcgttc gacgggaagt ccacgagcgt caggtcgacg
     3361 aatgccgggt agacggcctc ggccagcagg tcgcgatcga tggtcagagt caggacgtcg
     3421 ggcgactcct cgtccgccgc cggaacgacg gtcagcgtga cggcgctggc gcgtacgccg
     3481 ccgccgtcgc cttcgacgtc agacgagtcg agcagcatcg ggcgggtgac gcggccgtag
     3541 atggagccgt ccgcgtcgcg cagcgtcacg gagccatcgg gctctggcgt aagggtcaag
     3601 gtgccagctt cgaccgcgaa cgcgacttcg ttcgaagccg ggcggtcggg gaggatcaag
     3661 tacgtcttga agccgtcggc gcggggcagg atacgtaggg cccggtcgcg accaagggcg
     3721 ttccggtagt cggcgtagct gccgtcgaga gccagcaccg gctcctcggc gttgcggccg
     3781 acgtgcggca gccccagcga cacgttcagc tcctcaccgg ccgcgtttga gccagagagc
     3841 gtcaacaacc cgtcttcgga gtcagcttcg cgaagcgcca ggctcactgg cgccgcagaa
     3901 gcgaccgcca cgcccgcatc ctcgtcctcg ggaacgctga agcgaagatc gattggctgc
     3961 cagtcggtcg agtcggcagg caggtagtgg atcgggcggc caaagaactc ggtggtgaac
     4021 ttgccctcgt cggtctccca cgtgcgcgag ttcgcggtgc gaaggtcgac acgctcctca
     4081 ccgtcgatgg cgggccccgc caccagtgtc tcgtcggtga tctccggcga ccaggcaagg
     4141 ggcggcacga gcgaagccgg ttcctcgggc gcgtcgatgg gcgcgggaat gacgcccggc
     4201 acgaatggtc cgcgtttccc gttccagtcc acggcgaggt atgccgtcac cgggagggtg
     4261 atgccgttga gcgagtgcac ggtgacgccg ctcgtcaacg cgctgatgac gcggccgtcg
     4321 ccgaggtaga tcccgatgtg cgtcccgccg ttgtagacga cgagatcgcc gcgctcgacg
     4381 tcctcgaggt cgctcgtgag cagctcgcgg ccggcgaacc agcgctggta gccggcggcg
     4441 cgcattcgcc aaacaccgat ctgattgcct tcgttggcgt cggtgaatgc tcgataaacg
     4501 aggccagagc agtcaatgaa aaccggacct tcgctgccca ctcgataggg gtggccgatg
     4561 tactggcgtg cagcggcaat cacggcttcg acgcgctcgg catactcgga gagctcgggc
     4621 tgggcagtgg cccgcggggt gggtgtcgga tcgggcgccg gggttgcggt cgggcttggc
     4681 ttcggcttcg gcttggtcgt cccctcaggc gatggctgta cggtcggcgt cgggctcggg
     4741 gacgggctgg cagtgggctg cgccgcgtgc gccacggtca tagggagggc ggtcgtcagc
     4801 gcaaggagga acgctgctgc ggacaagcgg atccggcgcg acgaagacgt cgatatggcg
     4861 ttcacggtac cgctgtccgt gtacatgccg atccccctga ccccttcatt cgctgcttgc
     4921 tgggtgcgcc gcgaatggag ccga
//
LOCUS       DATLCO010000244         8334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8407622, whole genome shotgun sequence.
ACCESSION   DATLCO010000244 DATLCO010000000
VERSION     DATLCO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8407622"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VMZ33_05770"
     CDS             <1..252
                     /locus_tag="VMZ33_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16772.1"
                     /translation="VSEDDTSKAMNEAYVEAAGGEGPASDGNSFNAEPSGPSATAATG
                     PASTKDLLIYAGLMVALISAALLSVAWFARRYFADPLLR"
     gene            complement(196..1077)
                     /locus_tag="VMZ33_05775"
     CDS             complement(196..1077)
                     /locus_tag="VMZ33_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16773.1"
                     /translation="MEQVVAANRRTVRDRLNAPFWLAFAIVVVGYAVAFYVLRFLLPQ
                     AEIAGTLAALVLGGGAYVQRSIEQRWRTSRGPVVPLSGYQRPWYLLGAVGLVAVWLAA
                     SLVPGAQLCLGPVTAVASNLDTLIRLVPPAVAFAFGVVAGQRADRYGLLVVVFAVVGG
                     YLLAQLTQSLVITLATSPGVCGNPMVDPGVGPPIGVPPGFELPPDPRPIYLADNIVAL
                     FLDNPLVLLMPAAMLGLWRGTSGRLGAYVGSLLREVTPGDRQAIVDLAYETAASGQRS
                     AKERVGEVAAGEPGDRQ"
     gene            1126..4077
                     /gene="polA"
                     /locus_tag="VMZ33_05780"
     CDS             1126..4077
                     /gene="polA"
                     /locus_tag="VMZ33_05780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HUR16774.1"
                     /translation="MQRLMLFDSNSLIYRAYFALMNTPLTTSKGKLVNAVFGFWSIVL
                     RGFQDVKPDYVIAAFDLKGPTFRHEQFADYKATRREMPDDLKDQFPIVRELIEAFGIP
                     IYQLQGFEADDLIAALVKQAEAQDVATTIVSGDLDLLQLVSERTTLMTTRGGVQQTTF
                     YDPAKVMERYGLEPSQMIDFKALKGDTTDNIPGIAGVGEKTAAKLVQDYGSVDAIYER
                     IDTVLPEKLRAKLVDNREDVFRWRDLVTVRADLPIELDLETARLGDYDRDEVLRLFRE
                     YEFRSLVERLPGITGEEARAPGDMLREADRLAPIPAVMVAGRQSAAERAQSAPSAGGG
                     GMQLSLDFGASAAAGASTANGHAPLGEATDAVTTAVAQVEPPAAQLQTASQQGAREHL
                     IALLRQPEMAERFDAAGDVKAWLAAQPDLTLGVALTDHRPRRGDLLGLAVVDHTGRIV
                     AAGPNDAVAFADLVIASGKPLVGHELKQLLVWELSRRDPTGTGNVLREARNLPEVAFD
                     SQIAAYILNAALRSQTLGSIASERLEIELPQDGLLGGADHAALQATAVAAAREPLERD
                     LGSDAGLDKLLREIELPLIPVLATLEAAGVAIDRQALGQLSTAFSTEIARLEEEIYAD
                     VGHQFTIGSPKQLEQVLFYELNLPRGRRTKTGFSTEAAVLEDLRAAHPAIDKILEWRT
                     YTKLRSTYVESLPLLLDAKTGRLHTTFHQAVASTGRLSSTDPNLQNIPIRSELGRKIR
                     HTFVAGSPDVVLLAADYSQIELRILAHVSGDVHLRQAFEERADIHRETAARVLKKEPS
                     DVTSDERSMAKMVNFGLAYGMSDFGLSSRANIPRAEAQEFISSYFAAYSGISYYMMHI
                     KEVAREQGYVSTLFGRRRWIPELQARNGALRGAGERMAINMPIQGTAADIVKIAMIRL
                     YRRLLAEDTRARMLLQVHDELLLEVPRDELDQLVPVVREEMENAVRLDVPLTVDLKSG
                     DDWESLTPIPA"
     gene            4083..4973
                     /gene="mutM"
                     /locus_tag="VMZ33_05785"
     CDS             4083..4973
                     /gene="mutM"
                     /locus_tag="VMZ33_05785"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HUR16775.1"
                     /translation="MPELPEVETIARELRPLVVGRTIVDASFDWPNQVKHPAPDQFVP
                     AVRGREILAVERRAKWLVAPLGPARDGGEPAVLAIQVKMTGQLDVVPPDAPRDKHVHV
                     VFSLDNGKELRLRDTRKFGRVAVYAPEAAETILGELGPEPLDDEFTLVDFRRLLRRRK
                     GRLKTLLMNQEFLAGVGNIYADEALWRARLHPLRSPQNIRPDQERLLYASLRDVLNEA
                     IDRRGSSVDSYRAPEGAGNMQRFLNVYGRTGKPCPRCGRPTKRIVIGARSTHFCSWCQ
                     RLPMAERTTQVDKILTATST"
     gene            4974..6941
                     /locus_tag="VMZ33_05790"
     CDS             4974..6941
                     /locus_tag="VMZ33_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HUR16776.1"
                     /translation="MAIVRLEEVRREIGAQVILDSITAAIARGEKVGLVGANGAGKTT
                     LLRLVAGIDDPDTGRVHRASGLRLELLAQETNLDPTFMAAPTLRDAVRSGAGELLGLE
                     QRLRQLEAMGAVGVETAEYARLHDQFEVRGGYTLDVRVDSALSGLGFDREDWGRPPTQ
                     MSGGQQTRAALARALVSELDLLMLDEPTNHLDVTAIEWLEKTLSDRPGALLVAAHDRA
                     FLDAVVGRIWEIREHRLEAFRGNYSAYAVQREERDARARLNAENLTGGIARERELVQT
                     YRSHRKFAKMHEHERRLAALQDQASVSAAPQGRRTTRIALDGNGRAPVVRSGDVAVDL
                     EDLTVGFPSVPIAVARRLEARRGDKIGVVGPNGAGKTTLLRTLAGDLAPLGGLMRMGQ
                     GVHIGYLAQVRARPLPGATVLDAFAGATGLDSGPARTYLARFLFRGDDVQKPVEKLSG
                     GERSRLELAIVGVAGSNLLLLDEPTNHLDIPAREALETFLRETAATAIVVSHDRRLLE
                     GVCNQLWVVEQTAASGPAQIGRFPGGYREWRAAVGSGWSVESALANAPLIPRTAPAAS
                     LPKPKLRTKPTSARQTLSKDAYRRRRQVVEDDLTRLGLRKSQLELALNDSGIQQNFVE
                     LRRVTSELADVDAALALAEDAWLVLAEQAPR"
     gene            6938..7588
                     /gene="coaE"
                     /locus_tag="VMZ33_05795"
     CDS             6938..7588
                     /gene="coaE"
                     /locus_tag="VMZ33_05795"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HUR16777.1"
                     /translation="MTTRGQPFLIGLTGPIGCGKSTVARMLAEVGGTVVDADELARRA
                     TEPGRATLAQIRERFGDEVFAADGSMDRAAVAQVVFGDDAALRDLERIIHPEVRRLVE
                     QELEAAAEEDAPFVVIEAIKLIEGGLAERCDQVWIVDCSPQTQRERLVGRGAEASDID
                     RRLATQGQGLTGRLANLLEQASRPPSAVRIVVTEGPLDETRERVEDALADALEGHP"
     gene            7780..>8334
                     /locus_tag="VMZ33_05800"
     CDS             7780..>8334
                     /locus_tag="VMZ33_05800"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HUR16778.1"
                     /translation="MAAAGSRRAKGSRGQSLVEFAILVPILATILMIAVDVGRIYLGW
                     VSLTNVARIGANFASMNPDAWQGGGDTTVQARYRSLMQKDATNIDCVMPSVLPAPTFG
                     DTPQYALGSRVQVNLTCSFALVTPFLSTLVGDGAGHISVSSNAVFTIRTGSVDGVTIG
                     STAATATPTAFPTVFQTTLPTPTPT"
BASE COUNT         1477 a         2802 c         2762 g         1293 t
ORIGIN      
        1 gtcagcgaag acgacacgag caaggcaatg aacgaggctt acgtggaagc agctggaggc
       61 gaggggccag caagcgacgg caactccttc aatgcggagc cgagcggccc gtctgccacc
      121 gccgcaaccg gtcctgcgtc gaccaaggac ctgctgatct acgccggctt gatggtcgcg
      181 ctgatcagcg ccgccctact gtcggtcgcc tggttcgccc gccgctactt cgccgacccg
      241 ctccttcgct gacctctggc cgctggccgc ggtctcgtac gcaaggtcca cgatggcctg
      301 ccgatccccc ggagtgacct cgcgcagcag ggagccgacg taggcgccca aacgccccga
      361 cgtaccgcgc cacaaaccaa gcatcgccgc cggcatcaac agcaccagcg ggttgtcgag
      421 gaagagcgcc acgatgttgt ccgcgaggta gatggggcgg ggatcgggcg gtaactcgaa
      481 tccgggtgga acaccgatgg gcggcccaac ccccggatca accattggat tgccacaaac
      541 ccccgggctc gtggccagag tgatcacgag gctctgagtc agctgggcca gcaggtagcc
      601 gcccacgact gcgaatacga ccacgagcag gccatagcga tccgctcgtt gcccggccac
      661 gactccgaac gcgaacgcca cggccggcgg caccaggcga atcaacgtgt cgaggtttga
      721 ggcgactgcg gttacgggtc ccaggcagag ctgcgccccc ggcacgagcg acgcggcgag
      781 ccatacggcc accaggccga cggcccccag caggtaccac ggtcgctggt agcccgatag
      841 cggcacaaca gggccgcgtg acgtgcgcca gcgctgctcg atcgaccgtt gcacgtatgc
      901 gccgccgccc aagaccaggg cggccagagt gcccgcgatc tcggcttgcg gaaggagaaa
      961 gcgaagcaca tagaaggcca cggcatagcc gacgacgacg atcgcgaagg cgagccagaa
     1021 aggtgcgttg agccggtcgc ggactgttct tcgattggcc gctacgacct gttccactgc
     1081 gccacgataa ggggtccgca tagccccggc cgatagactt cgacagtgca gcggctgatg
     1141 ctcttcgact cgaacagcct gatctatcgg gcctatttcg cgctcatgaa cacgccgctc
     1201 acgacgagca aaggcaagct cgtgaatgcc gtgtttggct tttggagcat cgtcctgcgg
     1261 gggttccagg atgtgaagcc ggactacgtc atcgctgcgt tcgacctaaa gggaccaacg
     1321 ttccggcacg agcaattcgc cgactacaag gccacccgtc gtgagatgcc cgatgacctg
     1381 aaggaccagt ttcccatcgt ccgcgagctc atcgaggcgt tcggcatccc gatctaccag
     1441 ctgcagggct tcgaggccga cgacctcatt gccgcgctgg tgaagcaggc tgaggcacag
     1501 gatgtcgcga cgacgatcgt ctcgggcgac ctcgaccttc tccagctggt cagcgaacgc
     1561 acgacgctca tgacaactcg cggcggcgtc cagcaaacga cgttctatga cccagcgaag
     1621 gtgatggagc gttacgggct cgagccgagc cagatgatcg atttcaaggc gctcaagggc
     1681 gacaccacgg acaacattcc gggcatcgcc ggcgtcggcg agaagaccgc cgccaagctt
     1741 gtccaggact acggctccgt cgacgccatc tacgagcgca tcgacacggt cctgccggag
     1801 aagctgcgcg cgaagctcgt cgataaccgc gaagacgtct tccgctggcg cgatctggtt
     1861 accgtgcggg ccgatctccc gatcgaactc gaccttgaaa cggcgcgcct gggcgactac
     1921 gaccgcgatg aagtcctgcg gctgttccgg gagtacgagt tccgttcact ggttgagcga
     1981 ctgccgggca ttacgggcga agaggcccgt gccccgggcg acatgctgcg cgaagccgat
     2041 cgcctcgcgc ccatccccgc ggtcatggtt gccgggcgtc aatcagcggc ggaacgggca
     2101 cagtccgcgc cctcggccgg tggcggcggc atgcagctca gcctcgactt cggcgcatcc
     2161 gcggccgccg gcgcctccac ggctaacggg cacgctccgc tgggcgaggc gacagatgcc
     2221 gtaacgacgg ctgtcgcaca agtcgagcca cccgccgcgc agttgcagac ggcgagtcag
     2281 cagggcgccc gcgagcacct catcgcattg ctgcgtcagc ctgagatggc ggagcggttc
     2341 gatgcggccg gcgacgtcaa ggcatggctc gctgcccagc cggacctgac cctgggagtt
     2401 gcgctgaccg accaccgacc gcgccgcggc gacctcctcg gtctggctgt cgtcgaccac
     2461 acaggccgca tcgtggctgc cggacccaac gatgcggtcg cgttcgccga cctggtgatc
     2521 gcgtccggaa agccgctcgt cgggcacgag ctgaagcagc tcctcgtgtg ggagctctct
     2581 cgtcgtgatc caacggggac aggcaacgtt ctgcgtgagg ctcgcaactt gcccgaggtc
     2641 gcgttcgact cacagatcgc cgcgtacatc ctcaatgccg cgttgcgcag ccagacattg
     2701 ggcagcatcg cgtcggagcg tctcgagatc gagctgccac aggacgggct gctcggaggc
     2761 gccgaccatg ccgccctcca ggccacggcc gtagccgccg cgcgcgagcc gctcgagcgt
     2821 gacctgggca gcgacgccgg cctcgacaag ctactgcgcg agatcgagct gccgttgatc
     2881 cccgtcctgg cgacgctgga ggcggccggc gtcgcgatcg accgacaggc cctcggccag
     2941 ctgtcgaccg ccttcagcac ggagatcgcc cggttggagg aggagatcta cgcggatgtt
     3001 gggcaccagt tcaccatcgg gagtcccaag cagctcgagc aggtcctctt ctacgagctg
     3061 aacctaccgc gcgggcggcg gacgaagacg ggcttctcca cggaggccgc ggttctggag
     3121 gatctccgcg ccgcgcaccc ggctatcgac aagatcctcg agtggcgcac gtacaccaag
     3181 ctgcgcagca cctacgtcga gtcgctgcca ctcctgctcg acgcaaagac cggtcgactg
     3241 cacacgacgt tccaccaggc ggtggcctcg accggccgat tgagctcgac cgacccgaat
     3301 ctccagaaca tcccgatccg ctccgagctc gggcgcaaga tccgccacac gtttgtggcc
     3361 gggtcgcccg acgtcgtgct gctggccgcg gactacagcc agatcgagct gcggatcctg
     3421 gctcacgtga gcggcgacgt gcacctgcgt caagcattcg aggagcgcgc cgacatccac
     3481 cgcgagacgg cggcgcgggt gctcaagaag gaaccgtccg atgtcacgtc cgacgagcga
     3541 tccatggcca agatggtcaa cttcgggctg gcctacggca tgagcgactt cggcctgtcc
     3601 tcgcgcgcga acatcccgcg ggctgaggca caggagttca tcagctcgta cttcgccgcg
     3661 tattccggga tcagctacta catgatgcac atcaaggagg tcgcccgaga gcagggctac
     3721 gtgtccactc tcttcggccg gcgccgctgg attccagagc tgcaggcacg gaatggcgcg
     3781 cttcgtggag ccggggagcg gatggcgatc aacatgccga tccagggcac ggccgcggac
     3841 atcgtgaaga tcgcaatgat ccgtctttac cgccggctgc tggctgagga cactcgggcc
     3901 cggatgctcc tccaggtcca cgatgagctt ctgctcgagg tcccgcgcga cgagctggac
     3961 cagctggtgc ccgtggttcg tgaggagatg gagaacgccg tccggttgga cgtgccactc
     4021 accgtcgact tgaagagcgg cgacgactgg gaatcgctca cgccgatccc tgcgtaaccc
     4081 gaatgcctga gctgcccgag gtagagacga ttgcgcgcga gctgcgaccg ctggtcgttg
     4141 gccgaaccat cgtcgacgcc tcattcgact ggccgaacca ggtcaagcac cccgcgcccg
     4201 accagttcgt gccggccgtc cgtgggcggg agatcctcgc ggtcgagcgt cgcgcgaagt
     4261 ggctggtcgc gccccttggc ccggcgcgcg acggcggtga gccggcggtc ctcgcgatcc
     4321 aggtcaagat gaccggccag ctcgacgtgg ttcccccgga cgcgccgcgc gacaagcacg
     4381 ttcacgtcgt gttcagcctc gacaacggca aggagctgcg tctccgggac acgcgcaaat
     4441 ttgggcgcgt ggccgtctat gcgcccgagg ccgccgagac catcctgggt gagctcggtc
     4501 cggaacccct cgacgacgaa ttcacgctgg tcgatttccg gcgtctactt cgccgccgca
     4561 agggccgcct caagacactg ctgatgaacc aggagttcct ggcgggggtc ggcaacatct
     4621 atgccgacga ggcattgtgg cgagcgcggc tccacccact gcgcagccca cagaacatcc
     4681 ggcccgacca ggagcggctg ctctacgcgt cgttgcgcga cgtactcaac gaggcgattg
     4741 accggcgcgg ctcgtccgtc gatagctacc gcgcaccgga gggcgccggc aacatgcagc
     4801 gcttcctcaa tgtgtacggc cgcactggga aaccctgccc gcgctgcggt cgtcccacga
     4861 aacggatcgt gatcggcgcg cgcagcacac acttttgttc gtggtgccag cggctgccga
     4921 tggctgagcg cactacccag gtcgacaaga tcctgacggc aacgagcact tagatggcaa
     4981 tcgtccgcct cgaggaggta cgccgcgaga tcggcgcaca ggtgatcctg gattcgatca
     5041 cggccgccat tgcacgcggc gaaaaggtcg ggctggtcgg cgccaacggt gctggcaaga
     5101 caacgctgct gcgcctggtg gcggggatcg acgatcccga cacgggccgg gtgcatcgcg
     5161 cgtcgggcct gcggctggag ctgctcgcgc aggaaaccaa cctcgatccc acgttcatgg
     5221 ccgcgccgac gctccgcgac gccgtccgtt caggggccgg cgagctgctc ggactggagc
     5281 agcgactacg ccagttggag gcgatgggcg cggtaggagt ggaaacagcg gagtacgcgc
     5341 ggttgcacga ccagttcgag gttcggggtg gctacaccct cgatgtgcgc gtcgattccg
     5401 cgctttccgg gctggggttc gatcgcgagg attggggccg accgccgacc cagatgtcgg
     5461 gcggacagca gacgcgggca gcacttgctc gggcattggt atccgaactc gaccttctga
     5521 tgctcgacga gcccaccaac cacctcgacg tcaccgcgat cgaatggctt gagaagacgt
     5581 tgtcggaccg acccggagcc ctgctcgttg ccgcgcacga tcgggcattc ctcgacgcgg
     5641 tcgtcggtcg catctgggag atccgcgagc atcggctcga agccttccgc ggcaactact
     5701 cagcctatgc cgtgcaacgt gaggagcgcg acgccagagc gcgcttgaac gccgagaatc
     5761 tcaccggcgg gattgcgcgc gaacgcgagc tggtgcagac gtatcgcagc caccgcaagt
     5821 tcgccaagat gcacgagcac gaacgacgac tcgccgcgtt gcaggaccag gcgagtgtga
     5881 gcgccgcgcc acaggggcgg cggaccacga ggatcgccct ggatggcaac ggccgcgcgc
     5941 ctgttgtccg gtccggggac gtcgccgtgg acctcgagga tctgacggtt ggcttcccga
     6001 gcgtgccgat cgccgtcgcc cggcgactgg aggctcgtcg tggcgacaag atcggtgtcg
     6061 ttgggccaaa cggcgctggc aagacaacgt tgctgcggac cctcgccggc gacctcgcgc
     6121 cgctgggcgg cttgatgcgc atggggcagg gcgttcacat cggctacctc gcgcaggtcc
     6181 gggcgcggcc actcccgggc gcgaccgtgc tcgacgcttt cgccggcgcc accggacttg
     6241 attccgggcc agcgcgcacg tatctggccc gtttcctgtt ccgcggcgac gacgtgcaga
     6301 agcccgtcga aaagctctcc ggcggtgagc gctcgcgtct ggaactggcc atcgttggtg
     6361 tcgccggatc aaacctgctg ttgctcgacg agcccacgaa ccacctcgac atcccggcgc
     6421 gggaagcgct cgaaaccttc ctacgagaga cggccgccac cgcgatcgtc gtatcgcacg
     6481 atcggcggct gctggagggc gtctgcaacc agctctgggt cgttgagcag acggcagcgt
     6541 cgggcccggc ccagatcggg cgttttcccg gcggctaccg cgagtggcgc gcagccgtcg
     6601 gttctggttg gtccgtcgag tcggccctcg cgaatgcgcc gctaattccg cgaaccgcgc
     6661 cggccgcgtc gctgccgaag ccgaagctac gcacgaagcc gacgtccgca cgacaaacat
     6721 tgtccaagga cgcctaccgg cgccgacggc aggtcgtcga ggacgatctg acccgactcg
     6781 gactgcgcaa gagccagctc gagctggccc tgaatgactc gggcatccag cagaacttcg
     6841 tggagctgcg gcgcgtcacc agcgagctcg cggacgttga cgccgcactc gccctggcag
     6901 aagacgcgtg gctggtcctt gccgagcagg cgcctcgatg acgaccaggg ggcaaccgtt
     6961 tctgatcggc ctgaccgggc cgatcggctg tggcaagagc accgtcgcgc ggatgctcgc
     7021 cgaagtaggt ggcacggtcg ttgacgcgga cgagctcgct cgccgcgcga cggaaccggg
     7081 ccgcgccacg ctggcgcaga tacgtgagcg cttcggtgac gaagtcttcg ccgccgacgg
     7141 ctcgatggac cgggcggctg tcgcgcaggt cgtctttggg gacgatgcgg ccctgcgtga
     7201 cctcgagcgg atcattcacc cggaagttcg acgactggtt gagcaggagc tggaagccgc
     7261 ggccgaggaa gatgcgccct tcgtcgtcat cgaggccatc aagctgattg agggtggtct
     7321 tgccgagcgc tgcgatcagg tctggatcgt cgactgctcc ccccagactc aacgcgagcg
     7381 gctcgtcggc cgcggcgccg aggcgtcgga catcgaccgc cgactcgcaa cccaggggca
     7441 aggcctcacg ggccgactcg cgaacctctt ggagcaagca agccgacccc catcggccgt
     7501 tcgcatcgtg gtcaccgagg gcccgctgga cgagacgcgg gagcgagtcg aagacgccct
     7561 cgctgacgcg ctcgaggggc atccctgagg taacccgtcc ggagtgcaca agtccgcact
     7621 tgcaagggga ctttcgacgg gcgcgggcaa accatcgtgg gcgggtcgaa agaacgtcgc
     7681 tgcgcttgac tttgggccga gatagagggg gctcgtcaca gcgcagccca gcgatagcga
     7741 acaggaactc gattgagccg aggaattacc tcgacaccca tggcggccgc tggctcgaga
     7801 cgcgcgaaag gatcgcgcgg tcagagcctg gttgagttcg ccatcctcgt tccgattctc
     7861 gcgaccatcc tgatgatcgc ggtcgacgtg gggcgcatct acctcggctg ggtcagcctc
     7921 accaacgttg cccgcatcgg tgccaacttc gccagcatga accccgacgc gtggcagggc
     7981 ggcggcgaca ccacagttca ggctcgctac cgcagcctga tgcagaagga cgcaacgaac
     8041 atcgactgcg tgatgccgtc cgtgctaccg gcgccaacat ttggcgacac gccgcaatac
     8101 gcccttggct cacgcgttca ggtgaatctg acgtgctcct tcgccctggt gacgccgttc
     8161 ctttcgaccc tcgtcggcga cggtgcgggt catatcagcg tcagttccaa cgccgtattc
     8221 acgatccgca cgggatcggt cgacggcgtg acaattggct cgacggcagc cacggcgacc
     8281 ccaacggcgt tcccgactgt cttccagacg acattgccga cgccgacgcc gacg
//
LOCUS       DATLCO010000245         4953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8408343, whole genome shotgun sequence.
ACCESSION   DATLCO010000245 DATLCO010000000
VERSION     DATLCO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8408343"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1197
                     /gene="tuf"
                     /locus_tag="VMZ33_05805"
     CDS             1..1197
                     /gene="tuf"
                     /locus_tag="VMZ33_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HUR16779.1"
                     /translation="MAKKKFERNKPHVNIGTIGHIDHGKTSLTAAISKYLSLKGSAEY
                     RAFDSIDNAPEERERGITIAIAHVEYETDARHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVEVPYIVVFLNKVDMVDDEELLELVELEVRELLSK
                     YQFPGDDIPVIRGSALKALEAPNDPSDPAYAPIQELMDAVDSYIPTPERAVDKPFLMP
                     VEDVFGIKGRGTVATGRVERGVVKVGEEVELIGVKDTRKLVVTGVEMFKKLLDEGRAG
                     DNVGLLLRGIERDEIERGQVLAKGGSVKPHTKFVARVYVLTKEEGGRHTPFYNGYRPQ
                     FYLRTTDVTGSIGLTGGAEMVMPGDNVDMNVELITPIALETGQRFAIREGGHTVGAGA
                     ITEIVA"
     gene            1226..1534
                     /gene="rpsJ"
                     /locus_tag="VMZ33_05810"
     CDS             1226..1534
                     /gene="rpsJ"
                     /locus_tag="VMZ33_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_387996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HUR16780.1"
                     /translation="MARQRIRIRLKAYDHRLLDQSALQIVETAQRTGADVVGPVPLPT
                     RIEKFTVIRSPFIDKDSREQFEIRTHKRLIDILDPAAKTVDALMRLSLPAGVDIEIKM
                     "
     gene            1535..2170
                     /gene="rplC"
                     /locus_tag="VMZ33_05815"
     CDS             1535..2170
                     /gene="rplC"
                     /locus_tag="VMZ33_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HUR16781.1"
                     /translation="MAIGVIGRKVGMTSIFTAEGQSVPVSVVEIEPNTVTALRTSERD
                     GYTAVQLGAGKARRLTKPRLGQLKDLPQVATTREFRLDDISEYEVGQTLDVSLFEDGE
                     TVHVSGVSKGKGFSGTIKRHHFRRGPETHGSDSHRQPGSIGAGTYPGKVFKGTPMAGR
                     MGHDNITVKKLTIVRTDAERNLLLIKGSLPGARNAMVIVRKAQATQSKGGR"
     gene            2170..2796
                     /gene="rplD"
                     /locus_tag="VMZ33_05820"
     CDS             2170..2796
                     /gene="rplD"
                     /locus_tag="VMZ33_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HUR16782.1"
                     /translation="MPVTTKLFSKQGKELGTVDLPEVLFNADINEALIHQAVTAQLAG
                     RRIGTHDTKTRGEVRGGGAKPYRQKGTGRARQGSKRAPHYAGGGVVFGPHPRSYEQKM
                     PKRMKRAALHGALTAKYGDGAVRVVDELGLETPKTQTLVGFLAALNAGGRVLVVATGA
                     DANLELSARNLPRVVIIRADSLNVVDVLNADTLLITQPSLDTMAEVYA"
     gene            2793..3095
                     /gene="rplW"
                     /locus_tag="VMZ33_05825"
     CDS             2793..3095
                     /gene="rplW"
                     /locus_tag="VMZ33_05825"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HUR16783.1"
                     /translation="MTLMASEIVLMPVISEKSMDETQRGKYTFRVHSDANKLQIKDAI
                     EDLFKVAVIDVNVSTTKAKEKSRSRRRGRQIGWTSPWKKAVVTLAAGQKIEFFEGV"
     gene            3102..3932
                     /gene="rplB"
                     /locus_tag="VMZ33_05830"
     CDS             3102..3932
                     /gene="rplB"
                     /locus_tag="VMZ33_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HUR16784.1"
                     /translation="MPLRSYKPTSPARRFVTRSTFEEITTDKPHKPLLEPSTRINGRN
                     SQGRLTVRHRGGGEKRHYRRIDFKRDKWDVPARIATVEYDPNRSARIGLLHYRDGEKR
                     YMLLPNGLGVGDVVVAGPSAEARPGNALPLRNIPLGTTIHNIELVPGRGGQIVRSAGT
                     SAQLLAKEGDHAQVRLPSGEVRRVDMRCMATVGQVGNLDHENQSIGSAGRARHMGRRP
                     QVRGVVMNPRDHPHGGGEGKSPTGMPPKTPWGQPAMGHRTRRNKLTGKNIVRSRHRKS
                     "
     gene            3957..4241
                     /gene="rpsS"
                     /locus_tag="VMZ33_05835"
     CDS             3957..4241
                     /gene="rpsS"
                     /locus_tag="VMZ33_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HUR16785.1"
                     /translation="MSRSLKKGPFVEERLLGRVQTMNRKNEKKVLKTWSRASVIFPEF
                     VGHTIAVYNGKKHIPVYVTENMVGHRLGEFSPTRTFRGHGKHTERSSALK"
     gene            4249..4587
                     /gene="rplV"
                     /locus_tag="VMZ33_05840"
     CDS             4249..4587
                     /gene="rplV"
                     /locus_tag="VMZ33_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HUR16786.1"
                     /translation="MRVSATAKYLRHSTRKARLVTQSIVGLPATKAVSNLTHMPQGAA
                     RDVLKVVQSAAANAENNHDLEIDDLVVVQAIANEGPTLKRFRPRAQGRAFAIHKPMTH
                     ITVVLESKEA"
     gene            4592..>4953
                     /gene="rpsC"
                     /locus_tag="VMZ33_05845"
     CDS             4592..>4953
                     /gene="rpsC"
                     /locus_tag="VMZ33_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HUR16787.1"
                     /translation="MGRKVHPYGFRLGTSRTWNAKWYADKSYTDLLKEDITIRQLVAK
                     RLSNASVSLVDIERGINHVTVTVHTAKPGIVIGKGGANVEALRQQIGRMTTSKVKLEI
                     KEIRQPELDAVLVASNIA"
BASE COUNT          989 a         1685 c         1550 g          729 t
ORIGIN      
        1 atggccaaga agaagttcga gcgcaacaag ccgcacgtca atatcggcac catcggccac
       61 atcgaccacg gcaagacgag cctgaccgcg gcgatcagca agtacctgtc gctcaagggc
      121 tcggccgaat accgcgcctt cgactcgatc gacaacgcgc ccgaggagcg tgagcgcggc
      181 atcaccatcg ccatcgccca cgtcgagtac gagaccgacg cccggcacta cgcccacgtc
      241 gactgccccg gccacgccga ctacatcaag aacatgatca ccggcgccgc ccagatggac
      301 ggcgcgatcc tggtcgtggc cgcgaccgac gggcccatgc cccagacgcg cgagcacatc
      361 ctgctggcgc gccaggtcga agtgccctac atcgtggtct tcctgaacaa ggtcgacatg
      421 gtcgacgacg aggagctgct cgagctggtc gagctcgagg tgcgcgagct cctttccaag
      481 taccagttcc cgggcgacga catccccgtc atccgcggct cggcgctgaa ggcgctcgag
      541 gcacccaacg atccgtccga ccccgcctac gcgcccatcc aggagctgat ggatgccgtc
      601 gactcctaca tcccgacgcc tgagcgcgcc gtcgacaagc ccttcctgat gcccgtcgag
      661 gacgtcttcg ggatcaaggg ccgcggcacc gtggccacgg gccgtgtcga gcgcggcgtg
      721 gtcaaggtcg gcgaggaggt cgagctcatc ggcgtcaagg acacgcgcaa gctcgtggtc
      781 accggcgtcg agatgttcaa gaagctgctc gacgagggcc gcgccggcga caacgtgggc
      841 ctgctcctca gaggcatcga gcgcgacgag atcgagcgcg gccaggtcct ggccaagggt
      901 ggctcggtca agccgcacac caagttcgtg gcgcgcgtgt atgtcctgac caaggaagag
      961 ggtggccggc acacgccctt ctacaacggc taccggccgc agttctacct gcgcacgacc
     1021 gacgtcacgg gctccatcgg cctgaccggc ggggccgaga tggtcatgcc cggcgacaac
     1081 gtcgacatga acgtcgagct gatcacgccc atcgccctcg agaccggcca gcgcttcgcc
     1141 atccgcgagg gtggccacac cgtcggcgcc ggcgcgatca ccgagatcgt ggcttaggta
     1201 gccgaaagga agctaggtaa gacccgtggc caggcagcga atcaggatcc gcctcaaggc
     1261 atacgaccac cggctgttgg atcagtccgc gctccagatc gtggagacgg cccagcgcac
     1321 cggtgccgac gtagtgggcc ccgtgccgct gcctacccgg atcgagaagt tcacagtcat
     1381 ccgttcgccg ttcatcgaca aggacagccg cgagcagttc gagattcgaa ctcacaagcg
     1441 gctgatcgac atccttgatc cggcggccaa gacggttgac gcattgatgc gactcagcct
     1501 cccggctggc gtcgacatcg agatcaagat gtagatggct attggtgtaa tcggccgaaa
     1561 ggtcggaatg acgtcgatct tcacggcaga gggccagtcc gtgcccgtgt ccgtggttga
     1621 gatcgaaccg aatacggtca ccgcactgcg cacgtccgag cgcgacggct acacggccgt
     1681 ccagctcggt gcgggcaagg cgcgccgcct gaccaagccc cgtctgggcc agctcaagga
     1741 tttgccgcag gtcgcgacga cccgcgagtt ccgcctggac gatatctccg agtacgaggt
     1801 cggccagacg ctcgacgtca gcctcttcga ggatggcgag accgtccatg tgagcggcgt
     1861 gtcaaagggc aagggcttct cgggcacgat caagcgccat cacttccggc gcgggccgga
     1921 gacccacggt tccgactcgc accgccagcc cggctcgatc ggagccggca cgtacccggg
     1981 caaggtcttc aagggcacgc ccatggccgg tcggatgggc cacgacaaca tcaccgtcaa
     2041 gaagctgacc atcgtccgca cggacgccga gcgcaacctg ctcctcatca agggctcgct
     2101 tcccggcgcg cggaacgcca tggtcatcgt gcgcaaggcc caggccacgc aatcgaaggg
     2161 tggccgctga tgcccgtgac aacgaagctg ttcagcaagc agggcaagga gctcggcacg
     2221 gtcgatctgc ccgaggtgct cttcaacgcc gacataaacg aggcgctgat ccaccaggca
     2281 gtgaccgccc agctcgccgg ccgacgtatc ggcacgcacg acaccaagac gcgtggcgag
     2341 gttcgtggcg gtggcgccaa gccctaccgc cagaaaggga ccggccgcgc acgccagggc
     2401 tccaagcgtg ctccccacta cgccggtggt ggcgtggtct tcggtccgca cccacgtagc
     2461 tacgagcaga agatgcccaa gcgcatgaag cgtgccgctc tccacggcgc actcaccgcc
     2521 aagtacggag atggcgcggt tcgcgtcgtc gacgagctcg gcctcgagac gccgaagaca
     2581 cagacgctgg tcggttttct ggccgcgctc aacgccggcg gtcgtgtgtt ggtcgtcgcg
     2641 accggcgccg acgccaacct cgagctttcg gcacggaacc tgccgcgggt cgtgatcatc
     2701 cgggccgact cgctcaacgt tgtcgacgtc ctgaacgccg acacgctcct catcacgcag
     2761 ccgagcctcg acacgatggc tgaggtgtac gcatgacgct catggcttcg gagatcgtgc
     2821 tcatgcccgt catcagcgag aagtcgatgg acgagacgca gcgcggcaag tacacgttcc
     2881 gcgtccatag cgacgccaac aagctccaga tcaaggatgc gatcgaggac ctgttcaagg
     2941 tcgccgtgat cgacgtcaac gtctcgacca ccaaggccaa ggagaagtcg cgcagccgcc
     3001 gccggggacg ccagatcggc tggacgtcgc cgtggaagaa agctgtcgtg acgctggccg
     3061 cgggccagaa gatcgaattc ttcgaggggg tctaggaaca gatgccactg cgaagctaca
     3121 agcccacctc gcccgcgcgc cggttcgtta cccggtcgac gttcgaggag atcaccaccg
     3181 acaagccgca caagccgctg ctcgagcctt ctacgcgcat aaacggccgc aacagccagg
     3241 gtcgcctcac cgttcgccat cgcggcggcg gggagaagcg ccactaccgg cggatcgact
     3301 tcaagcgcga caagtgggac gttcccgcac gcattgcaac ggtcgaatac gacccgaatc
     3361 ggtcggcgcg gatcggcctg ctccactacc gcgacggcga gaagcgctac atgctcctgc
     3421 ccaacggctt gggcgttggt gacgtggtcg tcgccggacc aagcgccgag gctcgccccg
     3481 gcaacgcgtt gccgttgcgg aacattccgc tcggcaccac gatccacaac atcgagctcg
     3541 ttcccgggcg cggcggccag atcgtccgct ccgccggaac gtcggcccag ctcctcgcca
     3601 aggaaggcga tcacgcgcag gtgcgcctgc cgtcaggcga ggtacgccgc gtcgacatgc
     3661 gctgcatggc cacggtcggc caggttggca acctcgatca tgagaaccag agcatcggca
     3721 gcgcggggcg tgcccgacac atgggcaggc ggccgcaggt tcgtggcgtc gtcatgaacc
     3781 cgcgcgatca cccgcacggc ggcggcgaag gcaagtcgcc gaccggcatg ccacccaaga
     3841 cgccgtgggg ccagcccgcc atgggccacc gcacgcgccg caacaagctc accgggaaga
     3901 acatcgtgcg ctcccggcat cggaagagct aagagcgcct gaggtaatag agagatatgt
     3961 cacgttcact caagaaaggg cctttcgtcg aggagcgact cctcgggcgc gttcagacca
     4021 tgaaccgcaa gaacgagaag aaggttctca agacgtggtc tcgggcaagc gtgatcttcc
     4081 ccgagttcgt tggccacacg atcgccgtct acaacggcaa gaagcacatt ccggtctacg
     4141 tgaccgagaa catggtcgga catcgcctcg gcgagttctc gcctacccgc accttccgcg
     4201 gccacggcaa gcacaccgag cgctcttcgg cgctgaagta gacctttgat gcgtgtatcc
     4261 gctacagcca agtacctccg ccactcgacg cgcaaggcgc gccttgtcac gcagtccatc
     4321 gtcggcctgc ccgccaccaa ggcggtgtcc aacctgacgc acatgccgca gggcgcggca
     4381 cgtgatgtgc tgaaggtcgt ccagagcgct gccgccaacg cggagaacaa ccacgatctc
     4441 gagatcgacg atctcgtcgt cgtccaggcg atcgctaacg agggtccgac gctgaagcgc
     4501 ttccggcctc gtgcccaggg ccgcgcgttc gcgattcaca agccaatgac gcacataacc
     4561 gtcgtgctcg aaagcaagga ggcctaggaa catgggtcga aaggttcacc cgtacggctt
     4621 ccggctcggc acgtcccgca cctggaacgc caagtggtat gccgacaagt catataccga
     4681 cctgctgaag gaagacatca cgatccgcca gcttgtcgcc aagcgcctgt ccaacgccag
     4741 cgtcagcctc gttgacatcg agcgtggcat caaccacgtg accgtcaccg tgcacaccgc
     4801 caagccgggc atcgtcatcg gcaagggtgg cgccaacgtc gaggcgctgc gccagcagat
     4861 cggccgcatg acgacatcga aggtcaagct tgagatcaag gagatccgtc agcctgagct
     4921 ggacgctgtc ctcgtggcct cgaacatcgc cca
//
LOCUS       DATLCO010000246         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4898894, whole genome shotgun sequence.
ACCESSION   DATLCO010000246 DATLCO010000000
VERSION     DATLCO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4898894"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VMZ33_05850"
     CDS             <1..708
                     /locus_tag="VMZ33_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HUR16788.1"
                     /translation="AEFFRDEGRDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPN
                     LATEMAGLQERITSTKKGSITSLQAIFVPADDYTDPAPATTFAHLDSSIRLERYLTEL
                     GIYPSVDPLTSTSRVLDPNVVGQEHYDVARETQRVLQSYRDLQDIIAILGVDELSEEQ
                     KITVARARRLQRFMSQPMFVAEVFTGREGRYVPIKDTVASFKEILDGKADDLPEQAFF
                     LAGTIDDVRENAKKLAA"
     gene            709..1137
                     /locus_tag="VMZ33_05855"
     CDS             709..1137
                     /locus_tag="VMZ33_05855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HUR16789.1"
                     /translation="MPLLLEIVTPEKQAFSDQVDSVVCPGIEGELGVLPHHAPLLTTL
                     GFGELRIRRGGEEEFFAIAGGFLQVRPDKVVVMAETADMASEIDLEKALDARREAEQA
                     IAEGFEEPADLARARAALQRALLRIRVSERRRREGPRSRG"
     gene            1144..2487
                     /gene="murA"
                     /locus_tag="VMZ33_05860"
     CDS             1144..2487
                     /gene="murA"
                     /locus_tag="VMZ33_05860"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HUR16790.1"
                     /translation="MASDRRFHWEYEEPVEHQAYRVTGGQTLSGRVRVSGSKNAALKM
                     LAAATLTGERCRFTNVPEIEDVRVMVEVLRDLGAAVDHPEPNTYEVAAGDVEWLFVPL
                     EAAAKMRASFILLGPLLARFGRVIMSNPGGDRIGRRPVNLHVDAMRALGAEIDYRNGY
                     YFARSPGRLRGGEITFPIVTVMGTENAILGAVLADGRTTIRPAAREPEVDDLIAFLQK
                     MGAEVYRSDADTIIVDGRRRLRGAEHRVMSDRIEAGTFVVAAAVTGGEVTIESAPCDH
                     LGAFLDVLNDIGVPLACGKDSIEVKRGDRGPGEYSPRDIETAPYPGLATDLQSPTSVL
                     LSQARGTSHLYETIFEDRLDWLDQFKRLGANITKHDNHHASISGPSQLHAAEVEMGDL
                     RAGASLMLGALAAEGTSVIHGAHHVRRGYENIEGKFLGLGAKIERVSEGASATNS"
     gene            2484..>3045
                     /gene="mreB"
                     /locus_tag="VMZ33_05865"
     CDS             2484..>3045
                     /gene="mreB"
                     /locus_tag="VMZ33_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HUR16791.1"
                     /translation="MKIGIDLGTANILVYVKGKGIVAREPSVVAVSDDNHIVAVGEEA
                     REMIGRTPGNITAIRPMKDGVIADYVITEAMLRYFIKKAGRSVTKPDVMISVPSGVTS
                     VEKRAVRDAALKAGAKEAYLIEEPLAAAIGANVPISGPSGNMIIDIGGGTSEIAVIAL
                     GGIVVSHSLRVGGNKFDESIASYIRKK"
BASE COUNT          547 a          991 c         1000 g          507 t
ORIGIN      
        1 gcggaattct tccgcgatga gggtcgcgac gtgcttctct tcatcgacaa catcttccgc
       61 ttcacgcagg caggttctga ggtgtcggcc ctgctcggcc ggatgccgtc ggcggtcggt
      121 taccagccca acctggccac ggagatggcc gggctgcagg agcggatcac ctcgaccaag
      181 aagggttcga tcacatcgct tcaggcgatt ttcgtccccg ccgacgatta cacggacccg
      241 gcgccggcga ccactttcgc ccacctcgac tcgtcgatcc gcctcgagcg ctatctgacg
      301 gagctgggca tttacccgtc ggtcgacccc ctcacctcga cgtcgcgcgt acttgacccc
      361 aacgtggtcg gtcaggagca ctacgacgtc gcccgcgaaa cgcagcgcgt gcttcagagc
      421 taccgcgacc ttcaggacat catcgccatc cttggtgtcg acgagctgtc ggaggagcag
      481 aagatcaccg ttgcccgagc gcggcggctg cagcggttca tgagccagcc gatgttcgtg
      541 gccgaggtat tcaccggtcg cgagggtcgc tacgttccga tcaaggacac cgtggcgtcc
      601 ttcaaggaga ttctcgacgg caaggccgac gatttgcccg agcaggcatt cttcctggcc
      661 ggcacgatcg acgacgtccg tgaaaacgcg aagaagctgg ccgcctagat gccgctgcta
      721 ctggagatcg tcacgccgga gaagcaggcc ttctccgatc aggtcgactc cgtcgtgtgt
      781 cccgggatcg agggcgagct cggcgtattg ccgcaccacg cgccgctgct gacgacactc
      841 ggctttggcg agctccgcat ccgacgagga ggcgaagagg agttcttcgc catcgccgga
      901 ggcttcctgc aggttcggcc ggacaaggtc gtcgtcatgg ctgagacggc cgacatggca
      961 tccgagatcg acctcgagaa ggcactggat gcacgccgcg aggctgagca ggcaatcgcc
     1021 gagggctttg aggagccggc cgatttggct cgcgcccgag ccgcgctaca gcgggcgctg
     1081 ttgcgcatcc gggtctccga acgtcgccgc cgggagggcc cgcgctcgcg cggctaacga
     1141 tccttggcaa gcgatcggcg ctttcactgg gaatacgagg agccggttga gcaccaggcc
     1201 taccgggtaa ccggcggtca gacgctcagc ggtcgtgtgc gcgtatccgg cagcaagaac
     1261 gcggcactca agatgctcgc ggccgcgact ctgaccggcg aacgatgccg gttcacgaac
     1321 gtgcctgaga tcgaggatgt ccgagtcatg gttgaggtcc tgcgtgacct cggcgcggcc
     1381 gttgaccacc ccgagccgaa cacctacgag gtcgcagcgg gcgatgtcga gtggctattc
     1441 gtgccgctcg aggcggcggc caagatgcgc gcatcgttca tcctgctggg gccgctcctg
     1501 gcgcggttcg gccgggtgat catgagcaac cccggcggtg accgcattgg ccggcgcccg
     1561 gtcaacctcc acgtcgacgc aatgcgcgcc cttggcgctg agatcgacta tcgcaatggc
     1621 tattacttcg ctcggtcgcc cggccggctg cgcggcggcg agatcacgtt cccgatcgtg
     1681 actgtgatgg gcacggagaa cgcgattctg ggggccgtgc tcgccgatgg gcgcacgacc
     1741 atccgtcccg cggcgcgcga accggaggtc gacgacctga tcgcgttcct gcagaagatg
     1801 ggtgccgagg tttaccgctc cgacgccgac acgatcatcg tcgacggccg gcggcgccta
     1861 cgtggcgccg aacaccgcgt catgtcggac cgcattgaag cgggcacgtt cgtagtcgct
     1921 gcggcggtga cgggcggcga ggtaaccatc gagtcggcgc cgtgtgacca ccttggcgcg
     1981 ttcctcgacg tgctcaacga tatcggcgtg ccgctggcct gtggcaagga ctcgatcgag
     2041 gtcaagcgtg gcgatcgtgg cccaggcgag tactccccgc gcgacatcga gacggcgcca
     2101 tacccgggcc tcgcgacaga cctccagtcg ccgacgtcgg tgctcctcag ccaggcccgc
     2161 gggacgagcc acctgtacga aacgatcttc gaggaccgac tcgactggct cgaccagttc
     2221 aagcgcctcg gcgcaaatat caccaagcac gacaaccacc acgcctcgat cagcgggcct
     2281 tcgcagcttc atgccgctga agtcgagatg ggcgatctgc gggcgggcgc gtccttgatg
     2341 ttgggcgctc ttgcggcaga aggcacgagc gtcattcacg gcgcgcacca tgtccggcgg
     2401 gggtatgaga acatcgaagg caagttcctc gggctcggcg cgaagatcga gcgtgtctca
     2461 gagggggcat ccgccaccaa ctcatgaaaa tcgggattga tctcggcacg gccaacatcc
     2521 ttgtctacgt gaaaggcaag ggcatagttg cgcgcgagcc atcggtcgtc gcggtcagcg
     2581 acgacaacca cattgttgcc gtcggcgaag aggcacgcga gatgatcggc cgcaccccgg
     2641 gcaacatcac cgcgatccgg cccatgaagg acggggtcat cgccgactat gtgatcaccg
     2701 aggcgatgct gcgctacttc atcaagaagg ccggcaggtc cgtcacgaag cctgacgtga
     2761 tgatcagcgt cccgtcaggg gtcaccagcg tcgagaagcg tgccgtccgc gacgcagctc
     2821 tcaaggccgg cgccaaggag gcgtacctca tcgaggagcc gctcgctgcg gcgatcggcg
     2881 cgaacgtccc gatcagcggt ccgtccggga acatgatcat cgacatcggc ggcggcacga
     2941 gcgagatcgc cgtcatcgcc cttggcggca tcgtcgtctc gcacagcctg cgcgtcggcg
     3001 gcaacaaatt cgacgaatcg atcgcgagct acatccgcaa gaagt
//
LOCUS       DATLCO010000247         4797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2666315, whole genome shotgun sequence.
ACCESSION   DATLCO010000247 DATLCO010000000
VERSION     DATLCO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666315"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VMZ33_05870"
     CDS             complement(<1..467)
                     /locus_tag="VMZ33_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HUR16792.1"
                     /translation="MDTLIQAIVIGIVQGLTEFLPVSSSAHLILLPRVLGWDDEFLLS
                     PEFTVMLHVGTLAALIIYFWRDLIRYAAAFLRVLRDRRVGDDPERRVSVLLAASVIPG
                     ALVGVALESFIDSFFREQLLVVCALLVVGAVILFAAERLAAHTRDMADLKLTDA"
     gene            complement(601..2829)
                     /locus_tag="VMZ33_05875"
     CDS             complement(601..2829)
                     /locus_tag="VMZ33_05875"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HUR16793.1"
                     /translation="MDLAATIQWIIPIAGIAAVLFAGYLARDVLSRDTGTDAMRDVAS
                     TIHEGAVAFIRRQYTTIGLLAIFGAIVIGAVIAIVEGPEVADTDIFGVELGIRTGVAF
                     LVGAAASMASGIIGMYISVKSNVRTAAAARRSLVEAVQVAMRGGAVSGFLVVALSLLG
                     VWGIFVAYGGFTNVAQAPFLIVGFGFGASFVALFAQLGGGIYTKAADVGSDLVGKVEA
                     GIPEDDPRNAGVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVAAFLIAQTAGW
                     PHPETWIFFPLVVRGFGLLATIVAMYFVRGNEEEDPMNILNRGYWATTILSVVGLGIT
                     TYVMMQTGNDSVGAMGIPAWVWMFFAGIVGLATSVVFVYITQYYTAGGWRPVKEIAEA
                     SKTGPATNIISGTAVGFETTFVTAITIGVALLVSAFLGSQAGLINANGDNVGAIFGTA
                     VATMGMLMTTAYILAMDTFGPITDNAGGIAEFSKAEAGAREITDRLDAVGNTTKALTK
                     GYAIASASLAAFLLFSAYIDKVNLILQRQIDAGIREAGTLLESVNLANVDVFVAALIG
                     AMLVYFFSSLAIRAVGTTAQAIIVEVRRQFREMPGIMDYTQRPDYARVVDITTRAALR
                     QMVLPGLVAVATPIVVGLLLGHEAIAGMLMVGTISGVLLATVLNNGGGAWDNAKKYIE
                     SGHLKDDDGNVIGKGTPAHAAAVVGDTVGDPFKDTAGPSLHVLVKLLATITLVLAPLF
                     IR"
     gene            3019..3663
                     /gene="lepB"
                     /locus_tag="VMZ33_05880"
     CDS             3019..3663
                     /gene="lepB"
                     /locus_tag="VMZ33_05880"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HUR16794.1"
                     /translation="MTDEPDGTSSVSVEQASPRRTPTGCLIEVAETLILTIIIFFLIQ
                     TFVAQPYQVHQESMETTLLSGQNVLVDKLTPRFDSYSRGDIVVFTPVLRTSSCEAQPD
                     DPDVGQVTPYIKRVIGEPGDTVDLRAGGVFINGVQLDEPYIRGVDTRQLSDETSWVVQ
                     PDRLFVLGDNRMNSVDSRSESIGEVCVRDVIGRAWLRYWPLNALGILQTPVYPE"
     gene            3759..4376
                     /gene="lepB"
                     /locus_tag="VMZ33_05885"
     CDS             3759..4376
                     /gene="lepB"
                     /locus_tag="VMZ33_05885"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HUR16795.1"
                     /translation="MSRKPLGCLVEIAETLILTIVIFFLIQTFVAQPYRVEQESMRTT
                     LEPDQYVLVDKLTPRFDDYSRGDIVVFTPLLRTSSCEAEPDDPDVGEVTPYIKRVIGE
                     PGDTVELRDGDVFVNGARLNEAYIRGVDTRPLSGETSWLVQPDRLFVMGDNRQNSTDS
                     RSDSIGEICTRDVVGRAWMRYWPINTVGILPTPTYPDVPPGTAAR"
     gene            complement(4407..>4797)
                     /gene="ndk"
                     /locus_tag="VMZ33_05890"
     CDS             complement(4407..>4797)
                     /gene="ndk"
                     /locus_tag="VMZ33_05890"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HUR16796.1"
                     /translation="LVDRYERRGLRIAGLKLMWAERGIAEDHYSEHREKPFFGGLVEF
                     ITSGPLVAIAVEGPRAIETCRAINGATRPWDAAPGSIRGDFALEVGQNIVHASDGPEA
                     AEREVTLWFNPDELLDYSRDVDRSILG"
BASE COUNT          899 a         1512 c         1601 g          785 t
ORIGIN      
        1 gcatcggtga gtttgagatc ggccatgtcc cgggtgtggg cggcgagccg ctccgccgcg
       61 aacaggatga cggctccgac gacgagcagc gcacacacaa cgaggagctg ctcgcggaag
      121 aaggagtcga tgaagctttc aagggcgact ccgacgaggg cgccgggaat gacgctggct
      181 gccaggagga cgctgacgcg ccgctcagga tcgtcgccca cccgacgatc gcgcaggacg
      241 cggaggaacg cggcggcata acggatcagg tcgcgccaga agtagatgat gagcgcggcg
      301 agagtcccga cgtggagcat gacggtgaat tcggggctca ggaggaactc gtcatcccag
      361 cccaggaccc ggggcagcag gatgagatgc gccgacgacg agacgggcag gaactcagtc
      421 agaccctgaa cgatcccgat gacgattgcc tggatcagcg tgtccatcta ggtgtccgca
      481 ccgagcggcc gcggggtgcc tggaatcaga ccgcggaacc ggcttacgcc gactccgcgg
      541 aattgagttg cgtccatttg agagaaccca gcagctgaac gaaggcgctg ccggtccgag
      601 ctagcggatg aagagcggcg cgagcaccag ggtgatcgta gccagcagct tcacgaggac
      661 gtggagcgac gggccggcgg tgtccttgaa tgggtcgccg accgtgtcgc cgacaacggc
      721 ggcggcatgg gcaggtgtgc ccttgccgat tacgttgccg tcgtcatcct tcagatgacc
      781 cgactcgatg tacttcttgg cgttgtccca ggcgccaccg ccgttgttca acacggtcgc
      841 gagcagaacg cccgagatcg tgccgaccat gagcatgccg gcgatagcct cgtggccgag
      901 gagcaggccg accacgatcg gagtcgctac cgcgacgagg cctggcagca ccatctggcg
      961 cagagccgcg cgggtggtga tgtcgaccac gcgcgcgtag tcgggccgct gggtgtaatc
     1021 catgatgccc ggcatctcgc ggaactggcg gcgaacctcg acgatgatcg cctgagccgt
     1081 tgtgccaacg gcgcggatgg ccagcgagct gaagaagtag acgagcatcg cgccgatcaa
     1141 tgcggccacg aagacgtcaa cgttggcgag gttgaccgac tcgagcaagg tgcccgcctc
     1201 gcgaatgccg gcgtcaatct ggcgctgcag gatcaggttg accttgtcga tgtaggcgct
     1261 gaagagcagg aaagcggcca gtgacgcaga agcgatcgcg tagcccttgg tcagggcctt
     1321 ggtcgtgttg cccactgcgt cgagccggtc ggtgatctcc cgcgcaccag cctcggcctt
     1381 ggaaaactcg gcgatgccgc cggcgttgtc ggtgatcggg ccgaaggtgt ccatggccag
     1441 gatgtaggcc gtagtcatga gcatgcccat ggtcgcgacg gcggtcccga agatggcgcc
     1501 gacgttgtcc ccgttggcgt tgatgaggcc cgcctgggag cccaggaagg cgctgacgag
     1561 gagtgccacg ccgatcgtga tggccgtgac gaaggttgtc tcgaacccga cagccgtgcc
     1621 cgagatgatg ttcgttgccg ggcccgtctt cgatgcttcg gcgatctcct tgactggacg
     1681 ccagccgccc gcggtgtagt actgcgtgat gtagacgaac acaacgctcg tggcgagacc
     1741 gacgatgccg gcaaagaaca tccagaccca ggccggaatg cccatggcgc ccacggagtc
     1801 gttaccggtc tgcatcatca cgtacgtcgt gatgccgagg ccgacgactg acaggatggt
     1861 cgtcgcccag tagccgcggt tgaggatgtt catcggatcc tcttcttcgt tgccgcggac
     1921 gaagtacatc gccacgatcg tggccagcag gccgaagccg cgtacgacga gcgggaagaa
     1981 gatccacgtc tccgggtgcg gccagccggc ggtctgtgcg atcaggaacg cggcgacgcc
     2041 gaggatcatc gcgccgatgt tctcggctgc ggtggactcg aacaggtcgg cgccgcgacc
     2101 ggcgcagtcg ccgacgttgt cgcccacgag gtccgcgatc acgcccgcgt tgcgcgggtc
     2161 gtcttccggg atgcccgcct caaccttgcc tacgaggtcc gagccgacgt cggcggcctt
     2221 ggtgtagata ccgccgccga gctgtgcgaa gagagcaacg aacgaggcac cgaagccgaa
     2281 gccgacgatc aggaagggcg cctgcgcgac attggtgaag ccgccgtagg cgacgaagat
     2341 gccccacacg ccgaggagtg acagggccac gacgaggaag cccgacacgg cgcctccgcg
     2401 catggccacc tggaccgcct caacgaggct gcgtcgtgcg gccgccgcgg tgcgcacgtt
     2461 ggacttgacg ctgatgtaca tgccgatgat gccggatgcc atcgaagccg cagcgcctac
     2521 caggaaggcg acgcctgtcc tgattcccag ttcgactccg aagatgtcgg tgtcggcaac
     2581 ttccgggcct tcgactatgg cgatgaccgc gccgatgacg atggcgccaa aaatggccag
     2641 cagcccaatc gtggtgtact ggcggcgaat gaatgcgact gcaccctcat ggatcgtgct
     2701 cgccacgtca cgcattgcgt ccgtgccggt gtcgcgcgac aggacgtcac gtgccaggta
     2761 accggcaaac aacacggcgg caatgccggc gatggggatg atccactgga tcgtggcggc
     2821 gagatccacg cttgaagagc ctcctctagg tatctggtcc gggtcgaccg tatcggtcgg
     2881 gcgcgggcac gttgtcggcc tgcgacctca attttcgtcc gtggtcgtac ctggaaacgg
     2941 cgccggcata ggggaccgga gcgccgaggg cgagcggagt ttagcagagc gcagttggcg
     3001 ttgacgggtc gtccaagcgt gactgacgaa ccggacggta cgtcgtcagt atccgtcgaa
     3061 caagcctcac cacggcgaac acccacgggg tgtctgatcg aggtcgccga gaccctgatc
     3121 cttacgatca tcatcttctt cctgatccaa acctttgtgg cgcagcccta ccaggttcac
     3181 caagagagca tggagacgac cctgctatcc ggtcagaacg tcctcgtaga caagctcacg
     3241 ccgcgcttcg actcctattc gcggggtgac atcgtggtgt tcacgccggt gttgcgaact
     3301 tcgagctgcg aggcgcagcc cgatgatccc gatgttgggc aggtcacgcc ctacatcaag
     3361 cgcgtcatcg gcgaaccggg cgacacggtg gacctgcgcg cgggcggcgt gttcataaac
     3421 ggcgtccagc ttgacgagcc gtacatccgc ggcgtcgaca cgcggcaatt gagcgatgaa
     3481 acgagttggg ttgttcagcc cgatcgcctg ttcgtgctgg gcgacaaccg catgaactcc
     3541 gtcgactcgc gatccgagtc aatcggtgaa gtctgcgtca gggacgtcat cggccgggcg
     3601 tggctgcgct actggccgct gaacgcactc ggcattctgc agacgccggt ctacccggaa
     3661 tgagcttcac ggatcaatct cgggatccag acgagccagg tgcgccgggc cccgacaccc
     3721 agaaagatca cagcgaccgc cggtacactc ccccgcctgt gagccggaaa ccccttgggt
     3781 gtctcgtcga gatcgccgag acgctcatcc tgacaatcgt catcttcttc ctgatccaga
     3841 cgttcgtggc gcagccctac cgcgtcgagc aggagagcat gcgcacgacc ctcgagcccg
     3901 atcagtacgt gctcgtcgac aagctgacgc cgcgcttcga cgactattcg cggggcgaca
     3961 tcgtggtctt cacgccgctg ttgcggacgt cgagctgcga ggcagagccc gatgatcccg
     4021 acgtcggaga ggtcacgccc tacatcaagc gcgtcatcgg cgagcccgga gacacggtcg
     4081 agctgcgcga tggcgacgtg ttcgtcaatg gcgcccggct caatgaggcc tacatccgtg
     4141 gcgtggacac ccgacccctg agtggcgaga ccagttggct cgtgcagccc gaccggctgt
     4201 tcgtgatggg cgacaaccgc cagaattcga ccgattcgcg ctcggattcg atcggggaga
     4261 tctgcacacg cgacgtcgtc ggccgcgcgt ggatgcgcta ctggccgatc aataccgtcg
     4321 gcatcctgcc cacgccgacg taccccgacg tcccgccagg cactgccgca cgctagatgt
     4381 cgcgtgtcgg cgaaggacgc ctcgacctaa cccaggattg agcggtctac gtcgcgggag
     4441 tagtcgagca gctcgtctgg gttgaaccac aacgtcacct cgcgctcagc cgcctcaggc
     4501 ccgtccgatg cgtggacgat gttctgcccg acctcgagcg cgaagtcgcc gcggatggag
     4561 cccggcgccg cgtcccacgg gcgggtcgcg ccgttgattg cgcggcaggt ctcgatggcg
     4621 cggggcccct caacggcaat cgccacaagg ggtccagatg tgatgaactc aacgagcccg
     4681 ccgaagaacg gcttctcgcg atgctcggag tagtggtcct cagcaatgcc gcgctccgcc
     4741 cacatgagct tcaggccggc gattcgcaga ccacgccgtt catagcggtc gaccagc
//
LOCUS       DATLCO010000248         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6177926, whole genome shotgun sequence.
ACCESSION   DATLCO010000248 DATLCO010000000
VERSION     DATLCO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6177926"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VMZ33_05895"
     CDS             complement(<1..731)
                     /locus_tag="VMZ33_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HUR16797.1"
                     /translation="MAVAALLISVVPASAASDDYQNPLDMRVPGDGAVESCADPTVIR
                     SKTAGDRAWYMYCTTDPLNSADKTGNDFNFHLIPTFRSMDLVRWTYVGDAFTSRPSWA
                     EPTAGLWAPEIKFFNGLYYLYFGVTDVKPAISGEPDTCHDDNAIGVATSTSPTGPWTV
                     SPAPVVEPRRGGGGCNFFWTFDPDVITAQDSANLGQKFIYYGSYYGGVQVRQLSPDGL
                     TSNPATAVQVAIPNRYEGSEVIYRGG"
     gene            complement(1028..1103)
                     /locus_tag="VMZ33_05900"
     tRNA            complement(1028..1103)
                     /locus_tag="VMZ33_05900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1068..1070),aa:Arg,seq:gcg)
     gene            complement(1109..1200)
                     /locus_tag="VMZ33_05905"
     tRNA            complement(1109..1200)
                     /locus_tag="VMZ33_05905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1164..1166),aa:Ser,seq:gct)
     gene            1275..>2038
                     /locus_tag="VMZ33_05910"
     CDS             1275..>2038
                     /locus_tag="VMZ33_05910"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HUR16798.1"
                     /translation="MPKRVVLIGHPVSHSLSGELQQAAFDELGVDARYEPADVPLMTL
                     PETIQELRGDDYLGANITVPHKERVVSMMDRLTEEAQQTGAVDTITREGARLIGHNTD
                     VIGFRASLDSLIGKQKMPKAAVVIGAGGGARAAVYSLITAGFQRVIVFNRHLHRAESL
                     IRHFNRQASHMELRAMPWHESVIEAELAKTKVLINASALSNAGEQSPIPAELLAPDLF
                     VLDLMYQPAETRLLRDAKSAGASETLNGDTMLIHQTA"
BASE COUNT          391 a          645 c          643 g          359 t
ORIGIN      
        1 ccgccgcggt agatgacttc tgagccttcg taccgattgg gaatggccac ttggacggcc
       61 gtggctggat ttgaggtcaa cccgtccggg ctgagctggc gtacctgcac gccaccgtag
      121 tagctgccgt agtagatgaa cttctgcccc aggtttgcgc tgtcctgagc ggtaatgacg
      181 tccggatcga acgtccagaa gaagttgcag ccaccgccac cgcgacgcgg ctcgacgacg
      241 ggcgcaggcg acacggtcca gggaccggta ggcgaagtgg acgtcgcgac gccaatggcg
      301 ttgtcgtcgt ggcaggtgtc gggctcgcca ctgatcgccg gcttaacgtc ggtgacaccg
      361 aaataaaggt agtagagacc gttgaagaac ttgatctcgg gcgcccacag tcccgctgtc
      421 ggctctgccc acgatgggcg gctggtgaac gcgtcgccga cgtacgtcca acgaacgagg
      481 tccatagagc ggaacgtcgg gataaggtgg aagttgaagt cgttgcccgt cttgtcagcg
      541 ctgttgagcg gatcggtagt gcagtacatg taccaggctc tgtcgcccgc ggtcttgctc
      601 cggatgacgg ttggatctgc gcaggactcg accgcgccat ctccggggac ccgcatgtcg
      661 agcgggtttt gatagtcatc cgaggccgca ctcgccggca ccacggagat gaggagtgct
      721 gcaactgcca aggcggtgcc gagcacatgt ctcgaaaacg tcgcgcgccc gccagatccg
      781 tgagagacag gacggtgacc catgttcgct cctccttcgt cgccgcggct ggcaacgctg
      841 tgaccgaaat cagcggtttc gacgacgtga caatggtcgt catcaacgcg ctaacgccga
      901 gtgcgttatg gcgcttgacc atttcaggcg cataacagcc gaggcagttc cagcgccgac
      961 gccgcgaccg ctggacatgc gaaaaccctc ggggacgacc ccgagggctg atctgacgtc
     1021 atggatttgg cgcgcccggc gagactcgaa ctcacggcct ttaggtccgc aacctaacgc
     1081 tctatccact gagctacggg cgcaacgatg gcggagaggg agggattcga accctcgaag
     1141 caggttaccc cacttagcgg tttagcaaac cgccgcacta ggcctctatg cgacctctcc
     1201 caaaccggag gggcgatgat agcatcggcc tcctttccac ccgccctgac gggtcgttat
     1261 cactggtgtt ttgcgtgccc aaacgcgtcg ttctaatagg tcatccggta tcacattcgc
     1321 tctctggcga gctgcaacaa gcggctttcg acgagctcgg cgtcgatgcg cgctacgaac
     1381 ccgccgacgt accgctgatg accctgcccg aaaccatcca ggagctccgc ggcgacgact
     1441 acctcggcgc caacatcacg gtgccgcaca aggaacgcgt cgtgtcgatg atggatcgcc
     1501 taactgagga ggcgcagcag accggggccg tggacacgat cacgcgcgag ggcgcacgcc
     1561 tgatcggcca caacaccgac gtcatcggct tccgcgcgtc gctcgactcg ctcatcggca
     1621 agcagaaaat gcccaaggca gcggtcgtaa tcggggccgg cggtggcgct cgagcggctg
     1681 tctattcgtt gatcacggcc ggcttccagc gggtgatcgt cttcaaccgt cacctgcacc
     1741 gcgccgaaag cctgattcgt catttcaacc gccaggcctc gcacatggag ctgcgcgcga
     1801 tgccgtggca cgagtccgtg attgaggcgg agctggccaa gaccaaggtc ctgatcaacg
     1861 catcggcgtt gagtaacgcc ggcgagcagt cgccgatccc cgctgagctg ctggcgccgg
     1921 acctcttcgt cctcgacctg atgtaccagc cggcggagac gcggctgctc cgcgatgcca
     1981 agtcggcggg cgcgagcgag acgctcaacg gcgacacgat gctcatccac cagacggc
//
LOCUS       DATLCO010000249         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1928980, whole genome shotgun sequence.
ACCESSION   DATLCO010000249 DATLCO010000000
VERSION     DATLCO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928980"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VMZ33_05915"
     CDS             <1..991
                     /locus_tag="VMZ33_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16799.1"
                     /translation="LRPGRFSRKVHVGLPDVGGRKAILDVHSRGKPLGHEVDIELLAR
                     KTYGFSGAQLADLLNEAAIMAARRSGDTITNEDLHNGWLKVAVGTGRRRSMTERDRAI
                     IAAHEVGHAICGKVHGDTRRVEEISLFAHGEALGVTVSSQEDNDLPAESDLRARLVAL
                     MGGRAAEELLFHQVTPGAANDFEKATQIATSMVHRWGMGRDPRAAEFGTSGRGTLSFI
                     AAKRDGTLPTELQAAATRAIAAILDEAYDTARNTLIDKLDVLRRIGVYLVQQERIDGD
                     TFDALYDGKMDVPDAQIEWRPAAARPREWAAITAMAISNEKLNEGSVGASSSK"
     gene            complement(1218..2000)
                     /locus_tag="VMZ33_05920"
     CDS             complement(1218..2000)
                     /locus_tag="VMZ33_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HUR16800.1"
                     /translation="MGLLDGKRALIFGVANDHSIAWGIAKAFHEHGAQIGFSSLEMMI
                     DKRVRPLADSIGATFVEPSDVQSDADIERVFSRWSDEYGSLDILVHALAFAKKEDLEG
                     RFMDTSRDGFALALDISAYSMVALAQRAAPLMTNGGSMLTLSYYGAEKVVANYNVMGV
                     AKAALEASVRYLANDLGPDGIRVNAISAGPIRTLAASGVANFRKMYGIFPDLAPLRRN
                     VTLDDCGNTAVYLASDLSSGVTGETIYVDAGFNILGVPNPEP"
     gene            complement(2026..>2287)
                     /locus_tag="VMZ33_05925"
     CDS             complement(2026..>2287)
                     /locus_tag="VMZ33_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16801.1"
                     /translation="DWTLQLRTVPAAPVYDGSGTHGALIGSVAVPDGLAAGQSVELTV
                     NATAPAEAGSWLVKSDVRLVDDTLASDLGVVALQVGLTTTSP"
BASE COUNT          390 a          776 c          754 g          367 t
ORIGIN      
        1 cctccgcccc ggcaggttct cgcgaaaggt ccacgtcgga ctgccggacg tcggcggccg
       61 gaaggcgatt ctcgatgttc actcgcgggg caagccgctt gggcacgagg tcgatatcga
      121 gctgctggcc cgcaaaacct acggcttctc aggcgcgcag ctggccgacc tgctcaacga
      181 ggccgcgatc atggccgccc gtcgcagcgg cgacacgatc acgaacgaag atctccacaa
      241 tggctggctg aaggtcgccg tcggcacagg ccgccgccgc tcgatgaccg aacgcgaccg
      301 ggcgatcatt gccgcccacg aggtcggcca cgcgatctgc ggcaaggtac acggcgacac
      361 gcggcgtgtc gaagagatct cgctgttcgc ccacggtgag gcgctcggcg tcacggtctc
      421 gtcccaggag gacaacgacc tgccggcgga gtcagacctt cgtgcgcgcc tcgttgcgct
      481 gatgggcggc cgggccgccg aggagcttct tttccaccag gtgacgcccg gtgccgctaa
      541 cgacttcgag aaggccacgc agatcgccac gtcgatggtt caccgctggg gcatgggccg
      601 cgatccccgc gccgccgagt tcgggacctc cggccggggc accctgtcgt tcatcgccgc
      661 caagcgcgac gggacgttgc cgaccgagct gcaggccgct gccacgcgtg cgatcgccgc
      721 aatcctggac gaggcatacg acacagcgcg caacacgctc atcgacaagc tcgatgtcct
      781 gcgccggatc ggcgtctacc tggtccagca ggagcggatc gacggcgaca ccttcgacgc
      841 gctgtatgac ggcaagatgg acgttcccga cgcccagatc gagtggcgac ccgccgcggc
      901 tcgtccccgt gaatgggcag cgatcaccgc aatggcgatc tcgaacgaga agctcaatga
      961 gggctcggtc ggagcctcct cgagtaagtg acgttgggcg actgctcgct cacctagcgt
     1021 cccgtcgaac gaggccatcc gctccgcttt ggggacgttt ggcggagcat cgccttgctg
     1081 atcgggtcag tggtccgcgg aatgcggtca ccatccttgg cccaaggcgc ccggcggacg
     1141 cgttactcgc ccagcgagca tcaactgtcg actcacaggc cggcggtcac ggcgtgccgc
     1201 gaccagtcac gccgctctca gggctcgggg ttgggcactc ccaggatgtt gaagccggcg
     1261 tcgacgtaga tcgtctcgcc ggtcacaccg gatgagaggt cgctcgccag gtagacggcg
     1321 gtgttgccgc agtcgtcgag cgtgacgttc cgtctcagcg gcgccaggtc ggggaagatt
     1381 ccgtacatct tccggaagtt ggccacgccg ctggcggcca gcgtgcggat gggcccggct
     1441 gagatcgcgt tgacgcggat gccgtccgga ccgaggtcgt tggcgaggta gcgaactgac
     1501 gcctccaacg cggccttggc aacgcccatc acgttgtagt tcgcgacgac cttttcggcg
     1561 ccgtagtagg acagcgtcag catcgagccg ccattggtca tgagcggtgc cgcccgctgc
     1621 gccaacgcga ccatcgaata ggcgctgatg tcgagggcga gggcgaaacc gtcgcgcgac
     1681 gtgtccatga agcgcccctc gaggtcttcc ttcttggcga acgccaatgc gtggacgaga
     1741 atgtccagcg agccgtactc gtcgctccag cgtgagaaga cgcgctcgat gtccgcgtcg
     1801 ctctggacgt cggatggctc aacgaacgtc gcgccaatcg aatctgccag cggccgcacg
     1861 cgcttgtcga tcatcatctc gagcgacgaa aagccgatct gcgcgccgtg ctcgtggaac
     1921 gccttggcga tgccccaggc gatcgagtgg tcatttgcta cgccgaagat cagcgccctc
     1981 ttgccgtcaa gcagtcccat gagcggcgac gatagcagcc tcgcgctacg gcgaggtcgt
     2041 ggtcagcccg acctgcagcg ccacgacacc caggtccgac gccagagtgt cgtctactag
     2101 tcggacgtcc gacttcacca gccagctgcc tgcctccgcc ggcgcggtgg cgttgaccgt
     2161 aagctcgacc gactgcccgg ctgccaggcc atccggcacg gccacggacc caatcaacgc
     2221 tccgtgggtg cccgagccgt cgtacaccgg cgccgcggga acggtgcgca attgcagagt
     2281 ccaatcg
//
LOCUS       DATLCO010000250         2935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5658543, whole genome shotgun sequence.
ACCESSION   DATLCO010000250 DATLCO010000000
VERSION     DATLCO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5658543"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="VMZ33_05930"
     CDS             <1..1866
                     /locus_tag="VMZ33_05930"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HUR16802.1"
                     /translation="SRIDGFPRHLSIHVGGMLITAAPLIDIAPLERATMPGRVVVQYD
                     KRDIETIKLIKLDLLGLRMLSSIDDALRDIQADCAVVVDLDRLPEDIPEVFRMICAAD
                     TVGVFQIESRAQMQTLPRSRPENLDDLVVEVAIIRPGPIQGNAVHPYLRRRQGKEKVT
                     YLHPSLQPALEETLGVILYQEQVMKIAIDVAGFTAAESDGFRRAMGTWRSSREMEKLH
                     AQFVDGCIDRNGLTPEQAEELFRQVSAFASFGFNKSHAAAFARTAYESAFLKLFYPAQ
                     FVTGLINAQPMGFYPVEVLINDAKRHGVCVLPVDVNRSRYLTTTEWVGPPGTNLPPGV
                     GIAERPPIVQSSGCVVPDDASRGYWSATTAIEYGIRLGLHLVKGIGEAEGEALDVEIS
                     RAGPFASLADLVARTELSEEVVERLIRAGALDAFGEPRRKLLWQVREVAGATRASRTK
                     GVRPLDLRLPPTLAPELPAPSELERVGDAYAILSLDARRQVIELFRPALDSLGVTRAG
                     ELTDAKPGKVKIGGLVVTRQHPMTARGTVFLALEDETGMVNVTLWPDVWARFRRTVRR
                     HALLYVEGRLQRESDVVNVICDHVESLSEVARPAGGPAQPKGVRHMGQAGMRRLG"
     gene            complement(1927..2487)
                     /locus_tag="VMZ33_05935"
     CDS             complement(1927..2487)
                     /locus_tag="VMZ33_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16803.1"
                     /translation="MFKMPNFLGDIRALPGMFRAKPLLFLPLLILVIGFGIFLALPGL
                     SPDIASFGGLYIQFFWAPPALFTFFIAGFFAPRAAYLVGFVYGLLAGIMWASAVLLVG
                     SVGSTTGTPTTAAAEPVPIVLNMLVIGMLYGTLAAALASWYREFLRGIQERGRAKRVD
                     REVAERDKRRQERQEARRVAKQRPTT"
BASE COUNT          484 a          954 c         1003 g          494 t
ORIGIN      
        1 tcgcgcatcg atggattccc gcgacatctg tcgatccacg ttggtgggat gctcattact
       61 gcggcgcccc tgattgacat tgcgccgctt gaaagagcca cgatgccggg tcgtgtcgtg
      121 gtgcaatacg acaaacgcga catcgagacg ataaaactca tcaagctgga cctgctgggc
      181 ctccggatgc tgtcgtccat cgacgacgcc ctgagagata tccaggccga ctgcgccgtt
      241 gtcgtcgacc tcgaccggct gcccgaggac atccccgagg tgttccgcat gatctgcgcg
      301 gccgacacgg tgggcgtctt ccagatcgag agtcgggcgc agatgcagac gctgcctcgc
      361 tcccgcccgg agaacctgga cgatctcgtg gtcgaggtag cgatcatccg gcccggcccg
      421 atccagggca acgccgttca tccgtatctg agacgacgcc agggcaaaga gaaggtcacc
      481 tacctccatc cgagcctgca gccggcgctc gaggagacac tcggcgtgat tctctaccag
      541 gagcaggtga tgaagatcgc gattgatgtc gcgggcttca cggccgccga gtcggacggc
      601 ttccgacgcg ccatggggac gtggcgctcg agccgcgaga tggagaagct ccacgcccag
      661 ttcgtcgatg gctgtatcga ccgcaacggc ctcacgccag agcaggcgga agagctcttc
      721 cgccaggtat cggccttcgc gagcttcggc ttcaacaaga gccacgccgc tgcctttgct
      781 cggaccgctt acgagagcgc cttcttgaag ctgttctacc ccgcccagtt cgtgaccggg
      841 ttgatcaacg cccagccaat gggcttctac cccgtcgagg tgctgatcaa cgatgccaag
      901 cgacacggcg tgtgcgtgct gccggtcgac gtcaaccgca gccgctacct cacgacgaca
      961 gagtgggttg gaccgccagg caccaacctg ccacccgggg tcgggatcgc agagcgcccg
     1021 ccgatcgtcc agtcatcggg ctgtgtcgtg cccgatgacg ccagccgtgg ctactggtcc
     1081 gcaacgacgg cgatcgagta cggcatccgc ctcgggctgc atctcgtcaa ggggatcggc
     1141 gaggctgagg gcgaggcgct cgacgtggag atcagccgcg ccggcccgtt cgcctcgctg
     1201 gccgatcttg tggccaggac cgagctgtca gaggaggtcg ttgagcggct gattcgcgcc
     1261 ggcgcgctcg acgctttcgg cgagccgcgc cgaaagctgc tgtggcaggt tcgggaggtg
     1321 gcgggtgcca cgcgggccag ccggacgaag ggggttcggc cgctcgacct gcgactgcca
     1381 ccgaccttgg cgccggaact gcccgcaccg tcggagctgg agcgagtcgg cgatgcctac
     1441 gcgatcctgt cgctcgacgc tcggcgccag gtcatcgagc tgttccggcc ggcgcttgac
     1501 tcactcggcg tgacgcgcgc cggcgagctg accgacgcga agccgggcaa ggtgaagatc
     1561 ggcgggctcg tcgtcacgcg gcaacacccc atgaccgccc gcggcaccgt gtttctcgca
     1621 ctcgaggatg agaccggcat ggtcaacgtc acgctctggc cggatgtttg ggcgcgtttc
     1681 cggcggacgg ttcgtcgcca cgcgctgctc tacgttgagg ggcggctcca gcgcgagtcg
     1741 gacgtggtca acgtcatctg tgaccacgtc gagtctctgt ccgaggttgc gcggccggcc
     1801 ggtggaccgg cccagcctaa gggcgtgcgc cacatgggcc aggctggcat gcggcgcctg
     1861 ggttagtcgt gcgtcgcctc tgccggtccg gggcgccgcc ctgccagccg ggaggcgaat
     1921 ggccttctac gtagtcgggc gctgcttggc gacgcgccgg gcttcctgtc gctcctgccg
     1981 ccgcttgtcg cgctcagcga cttcgcgatc cacacgcttc gcgcggccac gctcctggat
     2041 gccgcgcagg aactcgcgat accaggacgc gagtgccgcc gcgagggtgc cgtagagcat
     2101 gccgatcacg agcatgttga gaacgatggg cacgggctcg gcagcggccg tagtcggcgt
     2161 gccggtcgta gagccaaccg aaccgaccag gaggacggcc gacgcccaca tgattcctgc
     2221 aagcagccca tagacgaagc cgacgaggta agccgctcgt ggcgcgaaga agcccgcgat
     2281 gaagaacgtg aacaaggctg gtggcgccca gaagaactgg atgtacaggc cgccgaacga
     2341 cgcgatgtcg ggcgagagcc cgggcagggc aaggaaaatt ccgaagccga tcaccaggat
     2401 caacagcggc aggaacagga gcggcttcgc tcggaacatg cccggcagcg cgcggatgtc
     2461 tcccaggaag ttgggcatct tgaacatcgg cttgcgctcg gtcgcaacgg gcgcggagcc
     2521 cgccgcctct ccatcggcgt cgtctatctc gtcgggcgaa gtcgcagcga ggtactcagc
     2581 gcgctgctcg gttcgggtcc gccggcgggc atcggcgcgg caggtgttct tctcgcgagc
     2641 cagggtgatt tctccttaga aagggtgtgc ggcggtcgca gcatcgtagc gcaacgacgc
     2701 tctgactccg ctggtgggtc cttgttcgcg acgcggtaag tacccggtaa ttggccggta
     2761 atcggcgcgt aaccgggtcg gccgagactg cgcacacctg tcgagccatc ccgccgtcgg
     2821 gagactcgga atgcttgtgc gccgcgcgct gctcatcgtt ttgttggtcg ttgtcgcgcc
     2881 gcaggttcgc ggcgcgcctg ccgttcaacc tgggataccg gaccttctgg ggcgc
//
LOCUS       DATLCO010000251         6890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9382238, whole genome shotgun sequence.
ACCESSION   DATLCO010000251 DATLCO010000000
VERSION     DATLCO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9382238"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VMZ33_05940"
     CDS             <1..691
                     /locus_tag="VMZ33_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16804.1"
                     /translation="EATFIARGAERAKSAAKGTDFEVVLEQLLGEVARATGDALENTA
                     SGAGDLMRSKKGDFVLTVDPQSCEGCDVRVVIEAKDRNMSWRELREELAAAKLNRSAQ
                     MAMVVFTPQHAPAGVAPFDVRSGHVLCVIDPEAPDVSTLAAALRLARLYAIASLERRA
                     DTIDVRRVSQAVAAVRGELDAVRALKTQLTAIGRTAAEVSTGLDRMRENVLSKVADAE
                     AYLRVGAAGRD"
     gene            complement(790..1365)
                     /locus_tag="VMZ33_05945"
     CDS             complement(790..1365)
                     /locus_tag="VMZ33_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUR16805.1"
                     /translation="MTAHQDVKTVIDSLSSRGYLRTSAVGAATITTLDGSTATPVPAA
                     VGGVVARGRIKTIVFATDLTPVSESAKIEAIDLAASLGARLIVVNVLDPGDAKRDLPS
                     RSTRLDQYRREREAPLLSIVMEAVARRVRATHMLWTGEPGQSIVAAAESEGADLIVVG
                     TRALDREGRFLLGSVSDHVVYHSRCPVLVAR"
     gene            1476..2963
                     /locus_tag="VMZ33_05950"
     CDS             1476..2963
                     /locus_tag="VMZ33_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HUR16806.1"
                     /translation="MVETQSTRGTLLPMHRLIADLEAWRAEPTPGRRDMVEQAVGELV
                     RAYSVRSIHLTVQSPAMPTLEVAIGTQPVPSAPEGSFALDGAPDAVVRLRIDADPLNR
                     EALKGALLVAFDGVVSRQEARFQRQQLEALDAAVRGIADVLSVDLVLQLIVDRVRELV
                     GAEYAALGIVGQFGNIEQFVTSGLSDEVRARIGPLPRGHGLLGLIIREDSSFLIDDIA
                     ADHRRHGFPPDHPEMHSFLGVPVRSKKLSIGNLYLTNKISAATFNESDLRLVEMFALH
                     AGIAMENARLHEEVQRLAIVDERQRISQDLHDSIIQSLYAISLSLEDLPEIFAEDPAE
                     GAARADRAIDSIHATTRDIRNFIFGLQPELLEDSDLRTGLATLATEFRTNALIDLEVV
                     IPTHLPALPDEHAAHVLAISREALSNVVKHSSASRAALEVAVRDGQMRLTVSDNGRGF
                     DAVAPRSLHQRGLANLRARAEAVGGTLAVTSSAISGTRIEAEIPL"
     gene            2972..3655
                     /locus_tag="VMZ33_05955"
     CDS             2972..3655
                     /locus_tag="VMZ33_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16807.1"
                     /translation="MTGEHRIDQAAEKPHRLLVVDDHEIVRQGLVSMLERRPGFQVVA
                     QAGTVAEAMEMARRHKPDLIVMDVRLPDGSGIEACREIRAEMPETRVLILTSYPDEEA
                     VFSAIVAGASGYLLKQVRGRDLVAALEALGRGESLLDPQVTEKVLERVRRIATGAADD
                     ELAQLTSQEKKILLLVAEGKTNKEIAADVFLSDKTVKNYVSSILSKLNLQRRAQAAAY
                     VAKRHNPGG"
     gene            3870..5210
                     /locus_tag="VMZ33_05960"
     CDS             3870..5210
                     /locus_tag="VMZ33_05960"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HUR16808.1"
                     /translation="MQSPVATSPGAAATASPSAHGSHTASPSGGGNGAGAAGTIEFHA
                     FDLGFEPAQLTVPAAGSYDVALVNDGAIAHDITFPDGTMVHAEAGATATGQVAIPAEG
                     LSFICSITGHADAGMRGVIAVEGDAAATPTATMNPDDHGGPAPATDVQPDPNAPVPVV
                     HDATAPALLEGETHDIELVMTEEEMTVATGFVQKVWTFGGTVPGPVIRVKVGDLIRVH
                     LVNPDTNEMAHSIDFHSSMVAWNDEMRSIAPGEELTYEWRANYAGVWMYHCGTAPALH
                     HIANGMFGMVIVEPEGGLGPVDAEFALVQNEWYLGPQGEPVDLSKAGAAAPAPDFVVF
                     NGVANQYKDAPIQVETGQRVRVFVLDAGPSVDSSFHIVGTIFDRVIKEGVELVSDNPG
                     GWGSQAVDLSPAQGAIVEFELAEDGLYPIVTHAFNFVGRGALGLFQAGDGDPTN"
     gene            5140..5547
                     /locus_tag="VMZ33_05965"
     CDS             5140..5547
                     /locus_tag="VMZ33_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HUR16809.1"
                     /translation="MPLTSSGAAHSACSRRGTATPRTERRGVWRAAGGLGDQRLGGAV
                     LDVIGLPIAGVILSVALLGLAVAWWRTDIYGLLLGHYGRSLVGVLFDVSFLYLQKFVI
                     KAVCIWCVTYEGSLLLRFLIAFAVWYRQPRPQS"
     gene            complement(5575..5970)
                     /locus_tag="VMZ33_05970"
     CDS             complement(5575..5970)
                     /locus_tag="VMZ33_05970"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HUR16810.1"
                     /translation="MDLGLILGIGLGVVLLWVVLLALFWLLRPRGVSARELAGTIPAV
                     LRLMRSLIGDRTVPPDVRGVLVGLLLWIISPIDLIPEFIPVLGPLDDVVVAIVAMRYV
                     RRRLGSEELRRRWTGTPEAFAIVARVIGT"
     gene            complement(5990..>6890)
                     /locus_tag="VMZ33_05975"
     CDS             complement(5990..>6890)
                     /locus_tag="VMZ33_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR16811.1"
                     /translation="ARHVETLPQSTPTGASDRVITLADGRHLGYHEIGDPHGRPVFFF
                     HGFGTSRVICPPDEGTALGMGVRLIAVDRPGIGLSDAKPGRSLLDWPQDVATLADRLG
                     IVTFSVIGWSGGGPYALACASALPDRVAAIGLVSSPAPLAGIAERGYLRRLDRNAVRA
                     AGRAPVLIRLALWHWGRPQRRDAERFFEESVAEMCAADQAILSDSALRERMIANSTEL
                     YRQGGRGMYDEALVLARPWGFELSDITVPVYLWHGERDETVPLGMATYLARAIPTATV
                     TFDSDEGHHLLYRRWPEILAALV"
BASE COUNT         1214 a         2286 c         2277 g         1113 t
ORIGIN      
        1 ggaggcaacc ttcatcgccc gtggcgcgga acgtgccaag tccgccgcga agggcacgga
       61 cttcgaggtt gtgctggagc agctgctcgg cgaggtggcc cgggcgactg gcgacgcgct
      121 ggagaacacc gcgtcaggtg caggcgacct catgcgctcc aaaaagggcg acttcgtgct
      181 caccgtcgac ccgcagagct gcgaaggctg tgacgtacgc gtcgtaatcg aggcaaaaga
      241 ccgaaacatg tcgtggcgcg agctgcgtga agagctcgcc gcggcaaagc tgaaccgcag
      301 cgcgcagatg gccatggtcg tgttcacgcc gcagcacgct ccagcgggcg ttgcgccgtt
      361 tgacgttcgc tccggccacg tcctgtgcgt cattgaccca gaggcgcccg acgtcagcac
      421 cctggcggcg gcgctccgcc tcgcgcggct ttacgccatc gcctcgctgg agcgcagggc
      481 cgacaccatc gacgtgcggc gcgtgtcgca ggccgtcgca gccgttcgcg gggagctcga
      541 cgcagtgcgg gcgctcaaga cgcagctgac ggccatcggc aggactgcgg ccgaggtctc
      601 caccgggctt gaccgaatgc gcgagaacgt gctgtcgaag gtcgctgacg cagaggccta
      661 tctgcgtgtc ggagccgccg ggcgcgacta gattgccgcg tcggatcggc tggccgaacg
      721 tcggcactgt cggcgtcggc gccgctatgc attcgccgat cggcggctcg cgcaggcgca
      781 acgcggccct cagcgcgcga ccagcaccgg acagcggctg tggtagacga cgtggtcgga
      841 cacgctgcca aggaggaagc ggccctcgcg gtcaagcgca cgcgtgccaa cgacgatcag
      901 gtcggcgcct tccgattccg cggcagccac gatgctctgt ccgggttcgc cggtccacaa
      961 catgtgggtc gcgcgcacac ggcgcgcgac agcctccatg acgatcgaaa gcagcggcgc
     1021 ctcacgctcc cttcggtatt gatcgagccg tgtcgatcgg gacggaaggt cgcgcttggc
     1081 gtcgccaggg tccagcacgt tgaccacgat gagacgcgcg cccagtgatg ccgccaggtc
     1141 gatcgcctcg atcttcgctg actcggatac gggcgtcagg tccgtcgcga agacgatcgt
     1201 cttgatgcgc cctcgcgcca ccaccccgcc gacggcggcg ggcacgggcg tagcggtgga
     1261 accatctaac gtcgtgatcg tcgccgcgcc cactgccgaa gtacgaaggt aaccacggct
     1321 cgagagactg tcgatgacgg tcttgacgtc ttggtgggca gtcactcgaa gaatgctgcg
     1381 gcatcgccgg catcttcaac agtgaccttc ggcccgatcc ggacgtgcct tgagacccgg
     1441 cggaaggcgt gccgcgcact ggtagattgg gcgaaatggt tgaaacgcag tcgacgcgcg
     1501 gcacgctctt gcccatgcat cgattgatcg ctgaccttga ggcatggcgt gccgaaccca
     1561 caccgggtcg gcgcgacatg gtcgagcagg cggtcggcga gctcgtccgc gcgtattccg
     1621 tgcgctcgat ccacctcacc gtccaatcgc cggccatgcc cacgcttgag gtcgccattg
     1681 ggacacagcc tgtgccatcg gccccggaag gctcattcgc ccttgacggc gcgcctgatg
     1741 ccgtggtccg gctccgcatc gacgcggacc ctctgaatcg tgaggcgctc aagggcgctt
     1801 tgctcgtagc ttttgacggc gtcgtctccc gacaagaagc tcgcttccaa agacagcaac
     1861 tggaggcgtt ggatgccgcc gtccgtggca tcgccgatgt cctctcggtc gaccttgttc
     1921 ttcaactgat cgtcgaccga gtgcgcgaac tcgtaggcgc ggagtacgcg gcgctgggca
     1981 tcgttggcca attcggcaac atcgagcagt tcgtcaccag cggcctgtcg gacgaagtca
     2041 gggccaggat cggccccctg ccgcgcggcc atggcctgct ggggctgatc atccgcgagg
     2101 acagctcgtt tctcattgac gacatcgcag cggaccatcg gcgccacggc ttcccgcccg
     2161 accaccccga gatgcactca tttctgggcg tccctgtccg cagcaagaaa ctctcgatcg
     2221 gcaatctgta cctgaccaac aagatcagcg cggcaacctt caacgagtcg gatttgcgcc
     2281 tggtagagat gttcgcgctc cacgccggca tcgccatgga gaacgcgcgg ctgcacgaag
     2341 aggtgcagcg gctcgcgatc gtcgatgagc gtcagcgcat cagccaggac ctgcacgact
     2401 cgatcatcca gagtctctac gccatctcgc tatcgttgga ggacctgcct gagatcttcg
     2461 ccgaggatcc ggcggaaggc gctgcgcgtg cggatcgtgc gatcgactca atccatgcca
     2521 ccacacgcga catccgcaac ttcatcttcg gcctccaacc ggaactcctg gaggactccg
     2581 atctccgaac cggcctggcg accctcgcca ccgagttccg gacaaatgcg ctgatcgacc
     2641 tcgaggtcgt gatccccacc cacctgccgg cgctccccga cgagcacgcg gcgcacgtcc
     2701 tggcgatcag ccgcgaagcg ctcagcaacg tcgtcaaaca ctcgagtgcg tcgcgcgccg
     2761 cgctcgaggt agcagtcagg gatggccaga tgcggctgac cgtgagcgac aacggccgcg
     2821 gcttcgacgc cgttgctccg agaagccttc accagcgtgg tcttgcgaac ctgagggccc
     2881 gcgcggaggc ggtcgggggc acgctggcgg ttaccagcag cgccatcagc ggcacccgaa
     2941 tcgaagccga gataccgctg taggagaaga gatgacaggc gaacaccgga tcgaccaggc
     3001 ggcggaaaaa ccgcaccgtc tgctggttgt cgacgatcat gagattgtcc gccagggtct
     3061 cgtgtccatg ctcgagcgcc ggcccggctt ccaggtcgtc gctcaggccg gcacggttgc
     3121 cgaagcgatg gagatggcgc gccgccacaa gcccgacctg atcgtcatgg acgtgcgcct
     3181 cccggacggg tctggtatcg aggcttgtcg ggagatccgc gccgagatgc cggaaacgcg
     3241 cgtactcata ctcacgagct atcccgacga ggaggccgtc ttctcggcga tcgttgccgg
     3301 agccagcggc tacctcctga agcaggtccg cggccgcgac ctggtcgccg cactcgaagc
     3361 cctcggccgc ggcgagtcgc tgctcgatcc ccaggtaacc gagaaggtgc ttgaacgcgt
     3421 acgccgcatc gccacgggcg ctgccgacga cgagctggcc caactcacgt cacaggagaa
     3481 gaagatcctg ctgctcgtag ccgagggcaa gaccaacaag gagattgccg ctgacgtttt
     3541 cctgtccgat aagaccgtga agaactacgt cagctcgatc ctctcaaagc tcaaccttca
     3601 gcgtcgcgcg caggcggcgg cgtacgtggc gaagaggcac aacccgggcg gctgatacga
     3661 ccagcgatgt gcaatcaagg ggccggatgg cacctgcgcc gcagtcgcgg agccgtcgac
     3721 catcgacggg accattccat tctccttggg gtacagccac gatgcgcgct tcgacaaagg
     3781 cactgtcggc tctctttctg atttttttag cgactggttg tgtgaatggc ggccgaccgg
     3841 cctggacatt cgccactcaa agcgccgcgt tgcaaagtcc tgttgccacg tcgcccggcg
     3901 cagcggcgac cgcttcccca agcgcacacg gcagccacac tgccagccct tcgggtggcg
     3961 ggaacggtgc cggagccgcc ggcacaatcg agtttcacgc cttcgacctg gggttcgaac
     4021 ctgcccagct gaccgtgccg gcagcgggca gctatgacgt cgcactggtc aacgacgggg
     4081 ctattgcgca cgacatcact ttccccgacg gcacgatggt ccacgctgag gcaggcgcga
     4141 ccgcaaccgg gcaagtggcg atcccggcgg agggcctttc gttcatctgc tcgattacgg
     4201 gccacgccga cgccggaatg cggggcgtaa ttgccgtcga aggcgatgcg gccgcgacgc
     4261 ccacggcgac gatgaacccg gacgatcacg gtggccccgc gcctgcgacg gacgtccagc
     4321 ccgatcccaa cgctccggtc cccgtcgtcc acgacgcgac cgcgccggcg ctgctcgagg
     4381 gcgagacgca cgacattgag ctggtcatga ccgaggagga gatgaccgtg gcgacgggct
     4441 ttgtccaaaa ggtctggacc ttcggtggga ccgtgccagg gccggtcatc cgcgtcaagg
     4501 tcggagacct cattcgagtt cacctggtca atccggacac aaacgagatg gcgcattcga
     4561 tcgacttcca ttcgagcatg gttgcgtgga acgacgagat gcgttccatc gctccgggtg
     4621 aggagctgac gtacgagtgg cgcgcgaatt acgccggcgt ctggatgtac cactgcggca
     4681 ccgcgccggc actgcatcac atcgcaaacg ggatgttcgg catggtcatc gtggagccgg
     4741 agggcggcct gggacccgtc gatgctgagt ttgccctggt ccaaaacgag tggtatctcg
     4801 gtccgcaagg tgagcccgtc gacctgtcga aggccggcgc cgcggcccct gcacccgact
     4861 tcgtcgtctt caatggcgtc gccaaccagt acaaggatgc tcccatccag gtcgaaaccg
     4921 gtcagcgcgt gcgggtgttc gtcctcgacg ccgggccgag cgtcgacagc tccttccata
     4981 tcgtcgggac gatcttcgac agggttatca aggaaggtgt cgagctcgtg tccgacaatc
     5041 ccggcggctg gggcagccag gccgtggacc tgtcacctgc acagggcgca atagtcgagt
     5101 tcgagctggc tgaagacggc ctgtatccga tcgtcacgca tgcctttaac ttcgtcgggc
     5161 gcggcgcact cggcctgttc caggcggggg acggcgaccc cacgaactga acgacgaggt
     5221 gtttggcgcg ccgctggcgg gttaggcgac cagcgactcg gcggcgccgt cctcgacgtc
     5281 atcggtctgc caatcgccgg cgtgatcctg tcggttgccc tgcttggcct ggcggttgca
     5341 tggtggcgta cggatattta tggccttctg ctcggtcact acgggcggtc gctggtcgga
     5401 gtgctcttcg acgtctcctt cctctacctg caaaagttcg tgatcaaggc cgtgtgcatc
     5461 tggtgtgtga cctacgaggg ctcgctgctg ctgcggttcc tgatcgcttt cgccgtgtgg
     5521 taccgccagc cacgaccgca gtcctgacca acgggctcac gcaaacacga cggctcatgt
     5581 gccgatgaca cgagcgacga tcgcaaacgc ctccggcgta cccgtccagc ggcgccgcag
     5641 ctcctcgctt ccgagccgac gacgcacgta cctcatggcc acgatggcca cgaccacatc
     5701 gtcgagtggt ccgagcaccg ggatgaattc cgggatcagg tcgatcgggc tgatgatcca
     5761 cagcagcagg ccgacaagca ctccgcgtac gtcgggaggc acggtgcggt cgccgatgag
     5821 ggaccgcatc aagcggagaa cggctgggat cgtccccgcc agctccctgg ccgaaacgcc
     5881 gcgcggccgc agaagccaga agagggcgag cagcaccacc cagagcagga cgacgccgag
     5941 gccgatgccg aggatgaggc ccagatccat ctggcgaccc gcgctagccc taaacgagcg
     6001 cggcgagtat ctccggccag cggcggtaga gcaggtggtg gccctcgtcg ctatcgaacg
     6061 tgacggtggc cgtcgggatg gcgcgcgcca ggtaagtggc catgcccagc ggaactgtct
     6121 cgtcacgctc gccgtgccac aggtaaaccg gcacggtgat gtccgacagc tcgaagcccc
     6181 acgggcgggc gagcacgagc gcttcgtcgt acattccgcg gccgccctgg cggtacagct
     6241 cagtcgagtt ggcgatcatc cgctcgcgca gagcgctgtc ggagagaatg gcctggtcgg
     6301 cggcgcacat ctcagcaacg gactcctcga agaagcgctc ggcatcgcgc cgttggggac
     6361 ggccccagtg ccagagcgcc agtcgaatca gcaccggggc gcggcccgcg gcgcggactg
     6421 cgttccgatc cagtcgtcgg agatagccgc gctcggcaat ccctgccagc ggcgcgggcg
     6481 agctcacgag gccgatggcc gcgacgcgat cgggtagcgc cgacgcgcag gcgagcgcgt
     6541 aggggccgcc gcccgaccac ccgatgacgg agaaggtgac gataccgaga cggtctgcca
     6601 gcgtcgcgac atcctgtggc cagtcgagga ggctgcggcc cggcttggca tcggacaggc
     6661 cgataccggg gcggtcgacc gcgatcagac ggacgcccat gccgagtgcg gtcccctcgt
     6721 cgggcgggca gatcacgcgc gacgtgccaa agccgtggaa gaagaacacc ggccggccgt
     6781 gcgggtcgcc gatttcgtga tagcccaggt gacgcccgtc cgcgagcgtg atcacccgat
     6841 ccgaagctcc cgtcggcgtg ctctgtggca atgtttcaac gtggcgcgcg
//
LOCUS       DATLCO010000252         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9382275, whole genome shotgun sequence.
ACCESSION   DATLCO010000252 DATLCO010000000
VERSION     DATLCO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9382275"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VMZ33_05980"
     CDS             <1..691
                     /locus_tag="VMZ33_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16812.1"
                     /translation="EATFIARGAERAKSAAKGTDFEVVLEQLLGEVARATGDALENTA
                     SGAGDLMRSKKGDFVLTVDPQSCEGCDVRVVIEAKDRNMSWRELREELAAAKLNRSAQ
                     MAMVVFTPQHAPAGVAPFDVRSGHVLCVIDPEAPDVSTLAAALRLARLYAIASLERRA
                     DTVDVRRVSQAVAAVRGELDAVRALKTQLTAIGRTAGEVSTGLDRMRENVLSKVADAE
                     AYLRVGAAARD"
     gene            complement(731..1126)
                     /locus_tag="VMZ33_05985"
     CDS             complement(731..1126)
                     /locus_tag="VMZ33_05985"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HUR16813.1"
                     /translation="MDLGLILGIGLGVVLLWVVLLALFWLLRPRGVSARELVGTIPAV
                     LRLVRSLIGDRTVPLDVRGVLIGLLLWIISPIDLIPEFIPVLGPLDDVVVAIVAMRYV
                     RRRLGSEELRRRWTGTPEAFAIVARVIGA"
     gene            complement(1146..>2046)
                     /locus_tag="VMZ33_05990"
     CDS             complement(1146..>2046)
                     /locus_tag="VMZ33_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR16814.1"
                     /translation="ARHVETLPQSTPTGASDRVITLADGRHLGYHEIGDPHGRPVFFF
                     HGFGTSRVICPPDEQTALGMGVRLIAVDRPGIGLSDAKPGRSLLDWPQDVATLADRLG
                     IVTFSVIGWSGGGPYALACASALPDRVAAIGLVSSPAPLAGIAERGYLRRLDRNAVRA
                     AGRAPVLIRLALWHWGRPQRRDAERFFEESVAEMCAADQAILSDTALRERMIANSTEL
                     YRQGGRGMYDEALVLARPWGFELSDITVPVYLWHGERDETVPLGMATYLARAIPTATV
                     TFDSDEGHHLLYRRWPEILAALL"
BASE COUNT          354 a          681 c          727 g          284 t
ORIGIN      
        1 ggaggcaacc ttcatcgccc gtggcgcgga acgtgccaag tccgccgcga agggcacgga
       61 cttcgaggtt gtgctggagc agctgctcgg cgaggtggcc cgggcgactg gcgacgcgct
      121 ggagaacacc gcgtcaggtg cgggcgacct catgcgctcc aagaagggcg acttcgtgct
      181 caccgtcgac ccgcagagct gcgaaggctg tgacgtacgc gtcgtgatcg aggcaaaaga
      241 ccgaaacatg tcgtggcgcg agctgcgtga agagctcgcc gcggcaaagc tgaaccgcag
      301 cgcgcagatg gccatggtcg tgttcacgcc acagcacgct cccgcgggcg ttgcgccgtt
      361 tgacgttcgc tccggccacg tcctgtgcgt cattgaccca gaggcgccag acgtcagcac
      421 cctggcggcg gcgctccgcc tcgcgcggct ttacgccatc gcctcgctcg agcgcagggc
      481 cgacaccgtt gacgtgcggc gcgtgtcgca ggccgtcgca gccgttcgcg gggagctcga
      541 cgcggtacgg gccctcaaga cgcagctgac ggccatcggc aggactgcgg gcgaggtctc
      601 caccgggctc gaccggatgc gcgagaacgt gctgtcgaag gtcgctgacg cagaggccta
      661 tctgcgtgtc ggagccgccg cgcgcgacta gtttgccgcg tcagatcgac tggctggcgc
      721 cggtcctcga ctaggcgccg atgacgcgag cgacgatcgc aaacgcctcc ggcgtgcccg
      781 tccagcggcg ccgtagctcc tcgctcccga gccgacgacg gacgtacctc atggccacga
      841 tggccacgac gacatcgtcg agtggtccga gcaccgggat gaattccgga atgaggtcga
      901 tcgggctgat gatccacagc agcaggccga taagcactcc gcgtacgtcg agaggcacgg
      961 tgcggtcgcc gatgagggac cgcaccaagc ggagaacggc tgggatcgtc ccgaccagct
     1021 ccctggccga aaccccgcgc ggccgcagaa gccagaagag ggcgagcagc accacccaga
     1081 gcaggacgac gcctaggccg atgccgagga tgaggcccag atccatctgg cgacccacgt
     1141 tacccctaaa ggagcgcggc gagtatctcc ggccagcgtc ggtagagcag gtggtggccc
     1201 tcgtcactat cgaaggtgac ggtggccgtc gggatggcgc gcgccaggta agtggccatt
     1261 cccagcggga ctgtctcgtc gcgctcgccg tgccacaggt aaaccggcac ggtgatgtcc
     1321 gacaactcga agccccacgg gcgggcgagc acgagtgctt cgtcgtacat tccgcggccg
     1381 ccctgacggt acagctcagt cgagttggcg atcatccgct cgcgcagagc ggtgtcggag
     1441 agaattgcct ggtcggcggc gcacatttcg gccacggact cctcgaagaa gcgctcggca
     1501 tcgcgccgtt ggggacggcc ccagtgccag agcgccagtc gaatcagcac cggggcgcgg
     1561 cccgcggcgc ggactgcgtt gcgatccagt cgtcggagat agccgcgctc ggcaatccct
     1621 gccagcggcg ccggcgagct cacgaggccg atggccgcga cgcgatcggg tagcgccgac
     1681 gcgcaggcga gcgcgtaggg gccgccgccc gaccacccga tgacggagaa ggtgacgata
     1741 ccgagacggt cggccagcgt cgcgacatcc tgtggccagt cgaggaggct gcggcccggc
     1801 ttggcatcgg acaggccgat accggggcgg tcgaccgcga tcagacggac gcccatcccg
     1861 agcgcggtct gctcgtcggg cgggcagatc acgcgcgacg tgccgaagcc gtggaagaag
     1921 aacaccggcc ggccgtgcgg gtcgccgatt tcgtgatagc ccaggtgacg cccgtccgcg
     1981 agcgtgatca cccgatccga agctcccgtc ggcgtgctct gtggcaatgt ttcaacgtgg
     2041 cgcgcg
//
LOCUS       DATLCO010000253         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9383019, whole genome shotgun sequence.
ACCESSION   DATLCO010000253 DATLCO010000000
VERSION     DATLCO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9383019"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VMZ33_05995"
     CDS             <1..1041
                     /locus_tag="VMZ33_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR16815.1"
                     /translation="MPMAIRDLVEEWFRSDSLRALVAARGVLLTGLGPRMPGTTGVLL
                     TDAAGADGGLAGQTVYARGGPGAVAEALAAAARASGVEIRGGVGVQRVKRLGEAATGV
                     ILDGGEEIDAAVVVSSLDPRTTLLTLLEPEVLGPRLSWRASNIRQRGVTAKVNYALAG
                     LPVFPGSDKDTRQLRGRIVLAPSMVALERATDPAKYGAQAEEPLIEATIPTLVDPSLI
                     DAAHAPAVRHVLSAVVQAAVDESIGDVATRTLEAYAPGFADLIVERQVFTPGDIEGEW
                     GAAGGHPMHAEVGLDQWLEWRPVHGFGRYRMPLRGMYLAGAGAHPGGGITGMPGYLAA
                     QAVLEDRGNRPR"
     gene            complement(1340..1558)
                     /locus_tag="VMZ33_06000"
     CDS             complement(1340..1558)
                     /locus_tag="VMZ33_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16816.1"
                     /translation="MAVDEAFGGRRAAAAPVPALNVVPQSLQNFAPGLFVAPQFGHPV
                     ANGVPHSLQNFAPSSFGDAQFGQITALL"
     gene            1584..>2767
                     /locus_tag="VMZ33_06005"
     CDS             1584..>2767
                     /locus_tag="VMZ33_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUR16817.1"
                     /translation="MTVLFADIVGSTPFAEAHDAEDVRDTMSRYFEMCSQVIGRYGGT
                     VEKFIGDAVMAVWGAPIAHEDDAERAVRAGLELVDAIGTIAPEFQARVGILTGEAAVT
                     LGATNQGMVAGDMVNTAGRLQSVAPAGSVLVGEATFRAASSSIVFEQAEDQLLKGKTS
                     PVPAWRAVRVVAELHGRNRRTGGLEAPFVGRTDELRLLKDLLHATGREQKTRLVSVMG
                     PGGIGKSRLAWEFSKYVDGLVENTYWHSGRSPAYGEGITFWALGEMVRGRCELVETDD
                     EETTRTKVRDTVAKWIEDTEKREWVEKSLVALLGLEADVASNQLFGAWRTFFEAISRD
                     GTVAMVFEDVHHADSGLLDFIDHMLEWSRGFPIYIVTLARPELLERRPDWGAAKRSFS
                     SV"
BASE COUNT          438 a          880 c          979 g          470 t
ORIGIN      
        1 atgccgatgg ccatccgcga tctcgtcgag gagtggttcc gcagcgattc gttgcgcgct
       61 ctcgtggcgg cacgcggcgt gctgctcacc gggcttgggc cgcgcatgcc ggggacgacc
      121 ggtgtgctgc tgaccgacgc agccggcgct gacggcgggc tggccgggca gacggtttac
      181 gctcgtggcg ggcccggcgc cgtggctgag gcactggcgg ctgcggcgcg ggcgtcgggt
      241 gtcgagatac ggggcggtgt gggtgtccag cgggtcaaac gactgggtga ggccgccacc
      301 ggggtgattc tcgacggcgg agaggaaatt gacgccgcgg tcgtggtgtc gtcgctcgat
      361 ccacgcacca cgctgttgac gctgctggaa ccggaggtgc ttggaccgcg gctgtcgtgg
      421 cgtgcctcga acatccgtca gcgcggagtg acggcgaagg tcaactacgc gctggcgggg
      481 ctgcccgtct tcccggggtc cgacaaggac acgcgacagc tccgcggccg catcgtgctc
      541 gcgccgtcga tggtggcgtt ggagcgtgcc accgatcccg cgaagtacgg cgcccaggct
      601 gaggaaccat tgatcgaggc gaccatcccc actctggtcg atccgtctct gatcgatgct
      661 gcgcacgccc cagcggttag gcatgttctg agcgcggtcg ttcaggccgc ggtcgacgag
      721 tccatcgggg acgtcgcgac acggaccctt gaggcgtacg cgccggggtt tgccgatctc
      781 atcgttgagc ggcaggtctt cacgcccggc gacatcgaag gggaatgggg cgcggccggc
      841 ggccatccga tgcacgccga ggtcggcctt gatcagtggc tcgagtggcg gcccgtgcat
      901 ggcttcggca gatatcggat gccgcttcga ggcatgtacc tggcgggcgc cggagcgcac
      961 cccggtggtg gcatcacagg aatgcctggc tacctggcgg ctcaggctgt gctcgaggac
     1021 cgcggcaatc gacctcgctg agcttgccca cccaagtgcc cttggtatcg ctcacatcgt
     1081 tagccaagcg gctctctgag cgtttggcct cggtgcagcg cggttcgtgc ggcggcgctg
     1141 ccgataaccg atctgaaacc gccgcttttc ccctgcatac cactccggca gggaccgaca
     1201 atgggcctcg cggtgtgtag gccaccgaag cttgctcagc taacgatgcc agcgatacgg
     1261 tgtcgagtgg cactcttcca cctgctcgcc tggcgactga ctgccgcact gctcgccctt
     1321 gctcggcctg atcgccgctt tacagtagag cggtgatctg tccgaactgc gcgtcgccaa
     1381 acgacgaggg agcgaagttc tgcaacgagt gcggcacgcc gttggcgact gggtgcccga
     1441 actgtggcgc cacgaacaag cctggcgcga agttctgcag cgactgcggc accacgttga
     1501 gcgccggaac cggcgcggcc gccgctcttc gcccgccaaa cgcctcgtca acggccaccg
     1561 accgtaccgc ggaacggcgc ttggtgacgg tcctgttcgc tgacatcgtc gggtccacgc
     1621 cattcgccga ggcgcacgac gccgaggacg tgcgcgacac gatgtcccgc tatttcgaga
     1681 tgtgctccca ggtcatcggt cgttacggcg gcacggtcga gaagttcatc ggcgacgcgg
     1741 tcatggctgt atggggcgct ccgatcgctc acgaggacga tgccgaacgc gcggtgcggg
     1801 caggtctcga gctggtcgac gccatcggga cgattgcgcc cgaattccag gcgcgcgtcg
     1861 ggatactgac gggcgaggcg gcggtcacgc tcggagccac gaaccaggga atggttgcag
     1921 gcgacatggt caacaccgcc gggcggctgc agtccgtcgc gccagccggg agcgtgctgg
     1981 tcggcgaggc cacctttcgc gcggcgagca gctcgatcgt cttcgaacag gctgaggacc
     2041 agctcctcaa aggcaagacc agtccggtgc ccgcctggcg tgcagtgcgg gtcgtagccg
     2101 agctccatgg ccgaaaccga cgaaccgggg gcctcgaggc tccgttcgtc gggcgcaccg
     2161 atgagctgcg gctgctcaag gacctcctgc acgcgaccgg gcgggagcag aagacgcgcc
     2221 tcgtctctgt catgggcccg ggcgggattg gcaagtcgcg cctggcctgg gaattctcga
     2281 agtacgtcga cgggctggtc gagaacacgt actggcacag tggtcgatcg cccgcctatg
     2341 gcgagggcat cacgttctgg gcgctgggcg agatggtccg cggccggtgc gagctcgtgg
     2401 agacggacga cgaagagaca acccgcacga aggttcgcga cacggtcgcc aagtggatcg
     2461 aggacaccga gaaacgcgag tgggtcgaaa aatcccttgt tgcgctgctt ggcctcgagg
     2521 cggacgtcgc ctcaaaccag ttgtttggag cctggcggac ctttttcgag gcgatctcgc
     2581 gggacggcac cgtggcgatg gttttcgagg acgtccacca tgccgactca gggctgctgg
     2641 acttcatcga ccacatgctc gaatggagcc gcggcttccc gatctacatc gtcaccctgg
     2701 cgcgcccaga actgctcgag aggcgccccg actggggtgc cgcgaagcgc agtttcagtt
     2761 ccgtcta
//
LOCUS       DATLCO010000254         4242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1936371, whole genome shotgun sequence.
ACCESSION   DATLCO010000254 DATLCO010000000
VERSION     DATLCO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936371"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..677)
                     /locus_tag="VMZ33_06010"
     CDS             complement(156..677)
                     /locus_tag="VMZ33_06010"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HUR16818.1"
                     /translation="MEPAYPPPAPPKKGINWIAIIAAIVIIGAIAGGFWLFRDRLSGS
                     ATDLTVGDCFDEPDLTTNITDVQHQPCADPHDGEVFAVLEYTPDGGSTTYPLIDTFRD
                     YVSDQCVPALESYTGRTGDDIFAAGFSFAYLYPSSESWSDGDREITCYITKEDAAKFS
                     GTLRGAGSSPSAT"
     gene            complement(930..1583)
                     /locus_tag="VMZ33_06015"
     CDS             complement(930..1583)
                     /locus_tag="VMZ33_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUR16819.1"
                     /translation="MGLFVWFAALLSRTALHLLASVKVEIDRDSLPKSGPLIVVANHI
                     SNADPPFVAGWLTPALGRQMHILAKRSLFIPVVGFILRRLGASPVKSGGSDIEAYRVA
                     LGILERGEILCIFPEGTRSANGEMGEPKPGVAMLATRSGVPILPVGVSGTDRFLGRGQ
                     RFPRLRAPITLRVGKPFTVDLDSTRSRREGMQAVSGEIMRRIADLTDERHRGPYGTS"
     gene            complement(1586..3157)
                     /gene="cmk"
                     /locus_tag="VMZ33_06020"
     CDS             complement(1586..3157)
                     /gene="cmk"
                     /locus_tag="VMZ33_06020"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HUR16820.1"
                     /translation="MNDADKRSISPRVRIQKALADAGVASRRKAEDLVATGRVAVNGE
                     PATIGQSVDPSADVIVVDGRPIRGDKPERIYLVLAKPAGVTSTVSDRHAVRTVIDLVP
                     VALRKRAARLYPVGRLDRDSEGLLLLTNDGDWAQRMLHPSHGVDREYAVAVDEPLDDD
                     QIDALETGVRFEEGDARIARMVQATSADVRVLSGLIGPHARRYVWYRVTLRQGMKRQI
                     RRMFVAVEAPVRRLVRLRFGTVRLARMQLGDVRALSAEERRQLEALVGGDTARDDKAR
                     SEKARAQKDQADRARDTNPPLRGLVVSVDGPGGSGKSTVGAGAAAKLGYRFCDTGVLY
                     RGLTWLALQRGVSTTDPDALAPLTGEIELAPDDQQRFRRLIIDGRDVTEELHTAEVDR
                     EVSAVSQHPQVRSNLMPIQHALAREGRIVMAGRDIGTIVLPDADPKIYLDVSIAERAR
                     RRAAARGLGDDPDAVAQIAEELRQRDDIDSHRETAPLRIPDGASVINTEENTLAETID
                     EVVNIVRRRERDLAK"
     gene            complement(3150..3773)
                     /gene="scpB"
                     /locus_tag="VMZ33_06025"
     CDS             complement(3150..3773)
                     /gene="scpB"
                     /locus_tag="VMZ33_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HUR16821.1"
                     /translation="MTTDELELVAVGPGAVEADAGADVEATTSDRPQAPAELTTEQLE
                     ALLFIAEKPLTRREIASLAGVDRGAVDARLGDLEVALANRGLRLLAVGEQVQLVTSPD
                     AGALIARYVGTEGVRLSPAALETLAIVAYRQPVTRAVIERIRGVDSDYVLRSLLHRRL
                     VVEQGRADTPGRPILYGTGFDFLERFGLTSLDELPTLDPEAAMRLSE"
     gene            complement(3763..>4242)
                     /locus_tag="VMZ33_06030"
     CDS             complement(3763..>4242)
                     /locus_tag="VMZ33_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HUR16822.1"
                     /translation="RRVIEYRMYRDAGRILAERLAIPVALFHREAGVAVAAGRANARP
                     SEERYDVRLLTTALAVSVRLIPPAPPPAEIVERKVTLEERASVIRAALRRVPTFVLQD
                     LLRDVSDRIVIAVTFLAMLELAKGREVTIEQEEPWGPISVRQLRDGSQPAPVTANDD"
BASE COUNT          693 a         1392 c         1433 g          724 t
ORIGIN      
        1 cttgccttct gcggcactga tgcgcgcata gtcgatggcc tcgcgcacgc cgtagcaaaa
       61 gccagtccgc cgcgcgattc ggacgtcttc gacggttccc atattcagtt caagtatggc
      121 cctacacggg ccatccggcc caagcagcca aacggttacg tggcggaggg cgaactcccg
      181 gcgccacgaa gcgtgccact gaacttggct gcgtcctcct tcgttatgta gcaggtgatc
      241 tcacgatcgc cgtctgacca gctttcggac gacggataga gataagcgaa gctgaagccg
      301 gccgcgaaga tgtcgtcgcc cgtgcggccc gtgtagctct caagcgcggg aacacattgg
      361 tcgctcacgt agtcgcggaa cgtgtcgatg agcgggtagg tggtcgagcc gccatccggc
      421 gtgtactcga gaacggcgaa gacctcgcca tcgtgcgggt cagcgcatgg ctgatgctgc
      481 acgtcggtga tgttggtcgt caggtccggc tcgtcgaagc agtcgccgac ggtcaaatcg
      541 gtagccgagc cgctgagtcg atctcgaaag agccagaatc caccggcgat ggcgccgatg
      601 atcacgatcg cggcgatgat cgcaatccag ttgatgccct tcttcggcgg cgcgggcggc
      661 ggataggctg gctccatagg gctgggataa gtgggctgct cggattgatc ccaacctggt
      721 tgagcggagg ctggcggctg cggtgcggcc caacccggct gggtcggatc cggttgctgc
      781 ggtgcagccc aaccgggctg ggtcggatct ggcggtgact gattcggcca tccctggccc
      841 ggcgtcggcg gtcccccggg cgtgccgggc gtggtcgggt cagacatcga tcctccattg
      901 acggtcttgc tgtgacggct cttcgcggat tatgaggttc cgtagggacc gcgatgacgc
      961 tcatcggtca aatcggctat gcggcgcatg atttcgccag aaacagcctg catcccctca
     1021 cgccgggacc tggtcgagtc aaggtcgacc gtgaatggct tgccgacgcg aagcgtgatc
     1081 ggcgcccgca ggcgagggaa tcgctggccg cgaccaagga atcggtcggt gccagagacc
     1141 ccaacgggaa ggatcggcac accggatcgc gtggcgagca tggccacgcc cggcttgggc
     1201 tcgcccatct ccccgttggc gctgcgcgtg ccctccggga agatgcacag gatctcgccg
     1261 cgctccagga tgcctagtgc gacgcggtaa gcctcgatat cgctgccgcc cgacttaacc
     1321 ggactggcac ccagccgtct gaggatgaat ccgacaactg ggataaagag cgaacgcttg
     1381 gcgaggatgt gcatctgccg gccgagcgcc ggcgtcagcc acccggcaac gaagggcgga
     1441 tcggcgtttg agatgtggtt ggcaaccacg atcagcgggc ccgattttgg aagcgagtcg
     1501 cggtctatct cgactttcac gctggcaagc aggtggagag ccgtccgcga gagcagcgcg
     1561 gcgaaccaga cgaacagtcc catccctact ttgccaaatc gcgctcgcgc cggcggacga
     1621 tgttgaccac ctcatcgatg gtctccgcga gcgtgttctc ctccgtgttg atcacggacg
     1681 cgccatcggg aatgcgcagc ggcgcagtct ctcgatggct atcgatgtcg tcgcgctggc
     1741 gcagctcttc ggcgatctgg gcaacggcgt cggggtcgtc gccgagccct ctggcggccg
     1801 cgcgacgtcg ggcacgttcc gcgatcgaga cgtccaggta gatcttcggg tcggcgtcgg
     1861 ggaggacgat ggtgccgatg tctcgcccgg ccatcacgat ccggccctcg cgggcaagcg
     1921 catgctggat cggcatgagg ttggaacgga cctgcgggtg ctgcgacacc gcgctgacct
     1981 cgcggtcgac ctcagcggtg tgcagctcct cggtcacatc gcggccgtca atgatgagcc
     2041 tccggaagcg ctgttggtcg tcgggagcga gctcgatttc ccccgtgagc ggcgccagcg
     2101 cgtcggggtc cgtggtcgac actccgcgct gtagggcgag ccacgtcagg ccgcggtaga
     2161 gaacgcccgt gtcgcagaac cgataaccga gcttggcggc ggcgccggct ccgaccgtgc
     2221 tcttcccgct gccaccgggg ccatcgacac tcacgacgag gccgcgcagc ggcggattcg
     2281 tgtccctggc cctgtcggcc tgatctttct gtgcccgggc cttctcggag cgggccttgt
     2341 cgtcgcgcgc cgtgtctccg ccgaccaacg cctcaagctg ccgtcgctcc tcggcgctga
     2401 gtgctcgaac gtcgccgagc tgcatcctgg ccaggcgcac cgtaccgaag cgcagcctca
     2461 cgagtcgtcg tacaggcgct tcgactgcga cgaacatgcg cctgatctgc cgtttcatcc
     2521 cctggcgcag cgtcacgcgg taccagacgt aacgacgcgc atggggtccg atgagaccgc
     2581 tgagcacgcg gacatcggcg ctggtcgcct gcaccatgcg cgcaatgcgc gcgtccccct
     2641 cctcaaaccg cacgcctgtc tcaagggcgt caatctgatc atcgtcgagc ggctcgtcga
     2701 cggccaccgc gtactcccgg tccacgccgt gactggggtg gagcatccgt tgagcccaat
     2761 caccatcgtt ggtgagcagc aacagtccct cggaatcgcg atccaggcgg ccgaccgggt
     2821 aaagccgggc ggcgcgctta cgcagagcga cagggacgag gtcgatcact gtgcgcactg
     2881 cgtgtcggtc gctcaccgtg ctggtgacgc cggccggttt agccagaacg aggtagatgc
     2941 gctccggttt gtcgcccctg atgggccggc cgtcgacgac gatcacatcg gcagagggat
     3001 cgacggactg accgatcgtg gccggttcac cgttgaccgc gacacggcct gtagcgacca
     3061 ggtcttccgc cttgcggcgc gaggcgaccc cagcatcggc cagcgccttc tggatgcgga
     3121 ccctcgggga aatgctccgc ttgtccgcgt cattcactga ggcgcatcgc cgcttcgggg
     3181 tcgagcgttg gcagctcatc gagcgacgtc agcccgaacc gctcaaggaa gtcgaagccg
     3241 gtcccgtaaa ggatcggacg gcccggcgtg tcggcccgcc cctgctcgac gacgagccga
     3301 cgatgcagca ggctccggag aacgtagtcg gagtcgacgc cccggatgcg ctcgatgacc
     3361 gcgcgggtga ccggctgccg ataggccacg atcgccaggg tctccagtgc cgcgggcgaa
     3421 agccggacgc cctccgtgcc gacatatcgg gcgatcagag ccccggcgtc aggcgacgtc
     3481 accagctgta cctgctcacc gacggcaagg agtcgaaggc cccgattggc cagggcgacc
     3541 tcgaggtctc ccaaccgtgc gtcaaccgca ccccgatcga ccccggcgag tgatgcgatc
     3601 tcgcggcgag tcagtggctt ctcggcaatg aagagcagtg cttcgagctg ttccgtcgtc
     3661 agttcggcgg gcgcttgcgg ccggtcggat gtggtcgctt cgacgtcggc cccggcatca
     3721 gcctcgacag cgcccggtcc gaccgcaacc agctcgagct cgtcagtcgt cattggcggt
     3781 caccggggca ggctggcttc cgtcgcgcag ctgcctgacg ctgatcggac cccacggctc
     3841 ctcctgctcg atcgtgacct cgcgtccctt ggccagctcc agcatggcca ggaaggtaac
     3901 ggcgatgacg atgcggtcgc tgacgtcccg tagcaggtcc tgcagcacaa acgtcggcac
     3961 gcgcctgagc gcagctcgga ttactgaggc gcgctcctcg agcgtgacct tccgttccac
     4021 gatctcagcg ggtggcgggg cgggcggaat gagacgtacg gagacggcta gtgccgtggt
     4081 cagaagccgg acgtcgtagc gctcctcgct gggccgggcg ttggcacgtc cggcagcgac
     4141 ggcgacgccc gcttcgcggt ggaacaatgc gacgggaatt gcgagccgct cggcgaggat
     4201 ccgacccgca tcgcggtaca tgcggtattc gatgacgcgc cg
//
LOCUS       DATLCO010000255         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4154553, whole genome shotgun sequence.
ACCESSION   DATLCO010000255 DATLCO010000000
VERSION     DATLCO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4154553"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..2204
                     /locus_tag="VMZ33_06035"
     CDS             342..2204
                     /locus_tag="VMZ33_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HUR16823.1"
                     /translation="MTTETAPAKEVETQELERVTIRFAGDSGDGMQLTGTQFTRTAAV
                     FGNDVSTLPDYPAEIRAPAGSLPGVSGFQISFSSADIYTPGDAPDVLVAMNPAALKTN
                     VGDLLPGGALIVNSDAFTPANLKKAGYDANPLTDGSLSSYNVFEIPISALNTRSAEGL
                     DMTSKQIDLTKNFFALGVMFWLYERSMEPTLRWIEQRFGTKPMVAEANTRALKAGYAF
                     GETTEMFHTTYRVAPAKLAPGTYRNITGNEATALGFVAAARLAKRELVYGSYPITPAS
                     DILHQLATYKNFGVKTFQAEDEIAAIGSAIGASYGGALGMTASSGPGIALKSEAMNLA
                     VMVELPLIVIDVQRAGPSTGMPTKTEQGDLLMAMFGRNSDSPVPIVAPATPAECFDYA
                     VEAARLALKYMTPVVYLSDAFLATGAEPWKIPSMADLPDISVSNPTDRATFQPYSRDP
                     ETLARPWAVPGTPGLEHRIGGLEKADVTGNVSYDPDNHHRMQVLRHEKIAGIARDIPN
                     LSVYGPENGDLLILGWGSTYGAIRSATERLQSEGWSVAHAHLRHLNPLPLNTEAVLRS
                     YRHILVPELNFGQLSLLLRGKFLIDVRGYNMVRGKPFRIVEIEQAAQQLLGENN"
BASE COUNT          420 a          749 c          664 g          384 t
ORIGIN      
        1 gacatcaccc agaccctcgc gaacgttgcc ctttgcctga tcgagcttgc cctcgttctc
       61 catctgctcg ttgcccatgg cgtctccgag gttctctttg acgtcgccgc gagcctcctc
      121 gaacttgcct tcaatctgat cgtcgttacc cagtaaagga cctccaatgc acggtcagga
      181 cccgtcactc gtgaaacggc gacggtttcc ttatgtaacg acgtccatca tgtgccgagg
      241 cgcattagga gcggaacaca cgcacccgtc ccagcctttc aacatcgtcc aaatccctcg
      301 tggggtgccc cgtgctcgcc cggctagaat caaaggacca catgacgact gaaaccgcac
      361 ccgctaagga agtcgagacc caagagctcg agcgggtaac tattcgattt gccggcgact
      421 cgggcgacgg catgcagctg accggtacgc agttcacgcg tacggccgcc gtgttcggca
      481 acgatgtcag cacgctgccg gactacccgg ctgagattcg ggcacccgcg ggctcgttgc
      541 ccggcgtgtc gggattccag atcagctttt catcggccga catctatacc ccgggcgacg
      601 cccctgacgt cctcgtggcc atgaacccgg ccgcgctgaa gacgaacgtc ggcgacctgc
      661 tgccgggcgg cgcgctcatc gtcaactccg atgcgttcac gccggccaac ctgaagaagg
      721 ccggctatga cgcgaacccg ctgaccgacg gctcgctcag ctcctacaac gtcttcgaga
      781 tcccgatcag cgccctgaac acgcgctcgg ccgagggcct ggacatgacg tccaagcaga
      841 tcgacctgac caagaacttc tttgcactgg gcgtgatgtt ctggctgtac gagcgctcga
      901 tggagcccac tctgcgctgg atcgagcagc gcttcggcac aaagccgatg gtcgccgagg
      961 ccaacacgcg cgccctgaag gcgggctacg cgttcggcga gacaacggaa atgttccaca
     1021 ccacgtaccg cgtggcgccg gcgaagctcg ctcctggcac ataccggaac atcaccggca
     1081 acgaggccac ggcactcggc tttgtagccg cagcgagact ggccaagcgt gagctggtct
     1141 acggctcgta cccgatcacg cccgcgtcgg acatcctgca ccagctcgcg acgtacaaga
     1201 acttcggcgt caagacgttc caggcagaag acgagattgc cgccatcggc tcggcgattg
     1261 gcgcttcgta cggtggcgcg ctcgggatga ctgcctcatc ggggccgggg atcgcgctca
     1321 aaagcgaggc aatgaatctg gcggttatgg tcgagctgcc gctgatcgtg atcgacgtcc
     1381 agcgcgccgg gccttcaacc gggatgccaa ccaagacaga acagggcgac ctgctgatgg
     1441 ccatgttcgg tcgcaactcc gactcaccgg tgccgatcgt ggctccggcc acacctgccg
     1501 agtgcttcga ttacgccgtc gaggccgcgc gactggcgct caagtacatg acgccggtgg
     1561 tctacctctc cgacgccttc ctcgcaacag gtgccgagcc ctggaagatc ccgtcgatgg
     1621 cagacctgcc cgatatctcg gtctcgaacc cgaccgatcg cgcgaccttc cagccctaca
     1681 gccgcgaccc cgaaacgctg gcgcggccct gggcggtgcc cggtacaccc ggactcgagc
     1741 accgcatcgg cggcctcgag aaggccgacg tcacgggcaa cgtgtcgtac gaccccgaca
     1801 accaccaccg gatgcaggtt ctgcgccacg agaagatcgc cgggatcgcg cgcgacatcc
     1861 cgaacctgtc cgtgtatgga ccggagaacg gcgacttgct gatcctcggc tggggatcta
     1921 cgtacggggc aatccgctcc gcgaccgagc ggctgcagtc cgaagggtgg agcgtcgcgc
     1981 acgcgcacct gagacatctc aatccgctgc ctttgaatac cgaggcggtg ctgcggtcgt
     2041 accgtcacat cctcgtgccg gagctcaact tcgggcagct gtcgttgctg ctccgtggca
     2101 agttcctgat cgatgtccgt ggctacaaca tggtccgcgg caagccgttc cgaatcgttg
     2161 agattgagca ggcagcccag cagctgctgg gggagaacaa ctgatggcgg tgaacgg
//
LOCUS       DATLCO010000256         3951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7676275, whole genome shotgun sequence.
ACCESSION   DATLCO010000256 DATLCO010000000
VERSION     DATLCO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7676275"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VMZ33_06040"
     CDS             <1..1085
                     /locus_tag="VMZ33_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HUR16824.1"
                     /translation="RSVEIILANQTSSGAFIASPTFSQYGFCWVRDGAFIAHALDLAD
                     QHEAAARFHDWMADVVIARRAGLARAADAGRRGEAPRPEDYLHCRYTASGAESVVEWP
                     AFQLDGPGIWLWSLGEHIVHGGQLTPRRAEAASLVADYLAALWPCPSYDAWEESPEHV
                     HTATVGAIAAGLRAVRGYLGPGLSAQRSAELAETADRAVRHLRTAAQPGYLPKWPGSG
                     AVDASLLWLGYPYALFRLDDPIYATTVARIEADLVSSGGGVYRYLEDTYFGGGEWLLL
                     TAALGSVYAARGAPGDSLKALRCLEWIEAQADSEGMLPEQVADHALHPTLTDDWVRSW
                     GPSARPLLWSHAAYLVLHRQLEAARL"
     gene            1082..2779
                     /locus_tag="VMZ33_06045"
     CDS             1082..2779
                     /locus_tag="VMZ33_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HUR16825.1"
                     /translation="MRGVGSAALRVVDFGPTRAWFKPGSEAGVKLAVDSPGGAVDVRL
                     ALFDGSRVVTANEARLRLPAGRSIRQFRLRVPEVQRHGYGLHLQLEGRSGRAEAHSAL
                     EALQGWWESPRHAALTDFQRGSAVVPTLKRLRRWHVTVAQFYDWMYRHYRYMPPPREE
                     DFADTLGRLVSHGVVKELVRQCRRNGIASLAYGSVYGAEREYVEGHPDELLRDAAGQP
                     LSLGGTFFITDLRSQSPWRRRLIGEYARACRRFGFDGIHMDQYGEPHEGFAADGSVVK
                     LQELFAGLIDEAADHLAKLSPPRRVLFNCVGGWPLEHVGRARSAATYIEIWPPDTTYS
                     AITRQIDIARRVAPTKQVIVAAYPSVLAADGDGGRPAGNALEAALLLTTVVLATGAYH
                     HVLAEHDRVLVGGYYPDAVSLPSRAAESMRLAWQFSARYVHLLSDTRNVPVPIDQLVI
                     EMDGRVLPVSSEPQAGAVWVRGTRTGDNEIAIQLVDLLGQVSDEWDAPRKAAVRRENW
                     RVSWRDGSQRPSLFASPWSYGGAMQQLSPGRVIPIPAFERWAMLITDDWPRSVGPTA"
     gene            complement(2776..3723)
                     /locus_tag="VMZ33_06050"
     CDS             complement(2776..3723)
                     /locus_tag="VMZ33_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HUR16826.1"
                     /translation="MPGKTFTNPVYDGYFADPFVLRLADRYFAYGTNDAQKSGKAFEI
                     LESVDLVHWQSIGRALDPVDGLDVRDHWAPEVAERDGIFYMYFSAGVEDREHRIRLAV
                     ADRPEGPFKYEGVVLTPDEPFAIDANPFRDDDGQWYLYYAHDVLEGERVGTSLAVDRL
                     VDMNTLAGEGHTIVRATADWQLFAHDRQMYGHTYDWHTVEGAHVVKRLGRYWCFYSGG
                     AWNGPTYGASWAVADSPLGPFTSAATDGPALLRTRPGEVHGPGHNSIVTTPAGVDYIV
                     YHAWDPALTARRMHIDRLEWTPDGPRTAGPTITPQPIPR"
     gene            complement(3724..>3951)
                     /locus_tag="VMZ33_06055"
     CDS             complement(3724..>3951)
                     /locus_tag="VMZ33_06055"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HUR16827.1"
                     /translation="ESVGGKLHGFWYAFGEYDGYTLWEAPDNGSMAAVAIALGSGGSL
                     SKVETTVLMTVDETMAAMARAGTVGYTRPGE"
BASE COUNT          608 a         1324 c         1309 g          710 t
ORIGIN      
        1 tgcgcagcgt cgagataatc ctggccaacc agacatcgtc gggcgcgttc attgcaagcc
       61 ccacgttttc gcaatatggc ttctgctggg tgcgggacgg tgctttcatt gcccacgctc
      121 tcgatttggc cgatcagcac gaggccgccg cgcgtttcca cgattggatg gccgatgtcg
      181 tgatcgcccg tcgggccgga ctcgcccggg cggcagacgc cgggcgacgc ggagaggctc
      241 cccggccgga ggactatctg cactgccgct ataccgccag tggggcggag agcgtggtcg
      301 agtggccggc gttccagctc gatgggcctg gcatctggct atggtcgctg ggcgagcaca
      361 tcgttcacgg aggccaactg accccgcgcc gtgccgaggc cgcgagcctg gtcgccgact
      421 atctggcagc gctgtggccg tgtccgtcgt acgacgcctg ggaagagagt cccgagcacg
      481 tccacaccgc caccgtgggc gcgatcgcag cgggacttcg agcggtccgc ggctacctgg
      541 ggcccggtct ctcagcgcag cgatcagccg aattggccga gacagcggat cgggccgtcc
      601 gccatctgcg cacagctgcg cagcccggct acctgccgaa gtggcccggt agcggcgccg
      661 tcgatgccag cctgctgtgg ctgggctacc cctacgcatt atttcggctg gacgacccga
      721 tttacgcgac taccgtggca cgcatcgagg ctgacctcgt ctcctccggc ggaggtgtct
      781 accgatacct cgaggacacc tacttcggcg gtggcgagtg gcttctgctc accgccgcac
      841 ttggcagcgt ctacgccgca cgcggcgcgc cgggggacag cctcaaggcg ctgcgctgcc
      901 tggagtggat cgaggcccag gcggattcgg agggcatgct gcccgagcag gtagcggacc
      961 acgctctgca ccccacgttg accgacgatt gggttcgctc gtggggcccc tccgcacggc
     1021 cactgttgtg gtctcacgcc gcgtacctgg tgctccacag gcagctcgaa gctgcccgat
     1081 tgtgaggggc gtcggctcgg cggctttgcg cgttgtcgac ttcggaccga cgagggcctg
     1141 gttcaagccg ggcagcgaag ccggcgtgaa gctggcggtc gacagccccg gcggggcggt
     1201 ggatgtgcgc cttgcgcttt tcgacggcag cagagtcgtg actgcgaacg aagcccggct
     1261 gcggctcccg gccggccggt cgattcgcca gttccgactc cgtgtccctg aggtccagcg
     1321 tcacggatat ggcctgcacc tgcagttgga gggtcggagc ggccgtgctg aagcgcactc
     1381 ggcactcgag gcactccagg gctggtggga atcgccgcgc catgcggcgc tgaccgattt
     1441 ccagcgcggg tcggctgtgg tgccgacgct caagcgcttg cgccgctggc atgtcaccgt
     1501 cgcccagttc tacgactgga tgtaccgcca ctaccggtac atgccgcccc cacgtgagga
     1561 ggacttcgcc gacacgctgg ggcggctcgt ttcccacggc gtggtcaaag agctcgtacg
     1621 tcaatgccgt cgcaacggca tagccagcct ggcctatggg tccgtgtatg gagcggaacg
     1681 cgagtacgtg gagggccacc ccgatgagct gctgcgagac gcagccggcc agccactcag
     1741 cctgggcggt acgttcttca tcaccgacct ccggtcgcag agcccatggc gacggcggct
     1801 gatcggcgaa tacgcacgtg cctgccgccg ctttggcttt gatggcattc acatggacca
     1861 gtacggcgag ccgcacgaag gtttcgcggc cgatggctct gtggtcaagc tccaggagct
     1921 gtttgcgggc ctgatcgacg aggccgccga ccaccttgcc aaactttctc ccccgcgccg
     1981 agtgctattt aactgcgtcg gaggctggcc gctcgagcat gttggccggg cacgcagcgc
     2041 agccacctac atcgagatct ggccccccga tacgacctac agtgcgatca cgaggcagat
     2101 cgacattgca cggcgcgtcg cgccgacgaa gcaggtcatc gtcgcggcct atccgtcggt
     2161 cctggctgct gacggcgatg gcggtcggcc ggcggggaat gcgcttgaag ccgcactcct
     2221 gctgacgacc gtcgttctgg caacgggtgc ctaccatcat gtcctggcgg aacacgatcg
     2281 cgtgctggtt ggcggttact accccgacgc cgtgtcactt ccctcccgtg ccgcggaatc
     2341 gatgaggctg gcgtggcagt tttccgcccg ctacgttcac ctgctgtccg acacccgcaa
     2401 cgtgccggtt cccatcgacc agcttgtgat cgagatggac gggcgcgttc ttccagtctc
     2461 ctctgagcct caagcaggtg cggtgtgggt ccgtggaact cggacgggcg acaacgagat
     2521 cgccattcag ttggtggacc ttctgggcca ggtttcggat gaatgggacg cgcctcgaaa
     2581 ggcagcggtc agacgcgaaa attggcgcgt ttcttggcgt gacggctccc aacgtccctc
     2641 gctttttgcc agcccgtggt cctacggtgg agctatgcag cagctttcgc ccggtcgagt
     2701 gattccgatt ccagccttcg agcgctgggc gatgctcatc accgatgatt ggccgcgtag
     2761 cgtggggccg accgcctagc gcggtatcgg ctgaggcgtg atcgtcgggc ccgccgtacg
     2821 cggcccgtcg ggcgtccatt cgagtcggtc gatgtgcatc cgccgcgccg taagcgccgg
     2881 atcccacgcg tggtacacga tgtagtcgac tccagcaggc gtggtcacga tcgagttgtg
     2941 gcccgggccg tgaacctcgc ccggccgcgt gcgtagcagc gccggcccat ctgttgccgc
     3001 gctcgtgaac ggaccgagcg ggctatccgc gactgcccac gacgccccgt acgtcggacc
     3061 gttccacgcg ccaccggagt agaagcacca gtagcggccc aagcgcttga ccacgtgcgc
     3121 cccctcgacc gtgtgccagt cgtacgtgtg cccgtacatc tgccggtcgt gcgcgaatag
     3181 ctgccaatca gcggtcgccc gcacgatggt gtgaccctct ccagcgagcg tgttcatgtc
     3241 gaccagccga tcgaccgcca gcgacgtccc gacccgctcg ccctccagca cgtcgtgcgc
     3301 gtagtagagg taccactgcc cgtcgtcgtc gcggaacggg ttggcgtcga tggcgaacgg
     3361 ctcatccggc gtcagcacta cgccttcgta cttgaaaggc ccctccggcc gatcagcaac
     3421 ggccagccga atccgatgct cccggtcctc cacgcctgcc gagaagtaca tgtagaaaat
     3481 tccgtcccgc tcggcgacct cgggcgccca gtggtcgcgg acatccagcc cgtcgacggg
     3541 gtcgagcgcc cggccgatcg actgccagtg caccaggtcg accgattcca ggatctcgaa
     3601 cgccttgccg ctcttctgag catcgttcgt gccgtaggcg aagtagcgat cggctaggcg
     3661 caggacgaat gggtcggcga aatagccgtc gtagaccggg ttggtaaatg tcttgcccgg
     3721 cacctattcg ccggggcgtg tgtatccgac tgtgcccgcg cgggccatcg ccgccatcgt
     3781 ctcgtcgacc gtcatcagga cggtggtctc caccttggag agcgaaccgc cgctgccgag
     3841 ggcgattgca accgccgcca ttgagccgtt gtcgggcgcc tcccagaggg tgtagccgtc
     3901 gtactcgccg aaggcatacc agaagccgtg gagcttgccg ccgactgatt c
//
LOCUS       DATLCO010000257         4505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3221078, whole genome shotgun sequence.
ACCESSION   DATLCO010000257 DATLCO010000000
VERSION     DATLCO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3221078"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VMZ33_06060"
     CDS             <1..306
                     /locus_tag="VMZ33_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16828.1"
                     /translation="LAHDSEYDETEYFDLVMEAREAVLRNFEEESLIEAIATELERKH
                     GFVHVENSLRAAVRVSGEEGETEVTPVVERAHGIDREADDFRTMLVEVEPEDRPYRN"
     gene            complement(319..1419)
                     /gene="ychF"
                     /locus_tag="VMZ33_06065"
     CDS             complement(319..1419)
                     /gene="ychF"
                     /locus_tag="VMZ33_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HUR16829.1"
                     /translation="MQVAIVGLARSGKTTVFNTLTRGQAETGGFGGMTVNVGVVKVPD
                     ERLTRLTEIFKPKREVPADVTYVDLPAPPTSADGREPAEIPADQLARLRTADELLHVV
                     RAFEDPSVPHPDGSVDPWRDVEQLDLEFVLADLSVIEKRIEKLSTSGRHGTPAERESN
                     ERELVVLEKLAPSLREGKPIRDVTLDDDEQKRIRGFRFLTEKPVLVLLNIGEADIARS
                     AEIAAEFAKRYQHRFSQVEALSAQVEMEIGQLDAEDAQVFRDEMGLTESSLERVIRLS
                     YQLLGLISFFTAGPDETRAWTIAAGSTAVDAAAAIHTDLARGFIRAEIVAYEDLIELK
                     SFAEARKNAKLRSEGKDYPMKDGEVVEIRFNV"
     gene            complement(1438..2502)
                     /locus_tag="VMZ33_06070"
     CDS             complement(1438..2502)
                     /locus_tag="VMZ33_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HUR16830.1"
                     /translation="MSPSSPQTQRLSGPGSGPGGVPAAIGLSPILSARYRQQDVERIA
                     AAAPGSRLVNVSMEGHADSDLDDVEVLLRGPLPMAVFDRLLLRSPRLMWVHSATAGVE
                     RVLTPLALERGLAITNARGVFSDPIAEYVLMWILAVSRRLPLLLELQKERTWQPLEGR
                     EMADVTVGIVGLGSIGRRVAEIVLTMGGQVVATKRRADFSDATPDEGDEDGIMPLIDL
                     LPADGLPELMARSDFVVLALPLTPETENTVDAELLAHAKPGAWLINIARGALVDDREL
                     IRAIREGRLGGAVLDAFREEPLPADSELYSVPNIIVTPHTSWSSGRVLDRSIDLFCQN
                     LELFRRGEPLLNRVDPNAGY"
     gene            complement(2499..3683)
                     /locus_tag="VMZ33_06075"
     CDS             complement(2499..3683)
                     /locus_tag="VMZ33_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HUR16831.1"
                     /translation="MARRNDRLVGVGLAIGAAGVAAIGSRYLSRKAQQVGDTVVDWRK
                     AEQIAIARLAAAPGALTQQQLRTAEHAYARAMRGVVPLLEQRLGFALPGVVERHAVVD
                     RAGWARANIATFQQLIGHLEPHIRSVTAGHGFAADVARLINRFATTQQVGFLLGYLGT
                     RVLGQYDIALLSAEAQPGKLLFVDENIRATAATLDVSLDDFRVWIALHETTHAYEFEA
                     NTWLRPYLRDRLERQLVGVLDQAKSMQSEGLGTLLRRLRESSDNPLVGLLGPEQRRLF
                     EETQRVMSLLEGFSDWVMDEVGSEVLPNVTVIREKFDARRYQRRGTLDRVIARLTGLD
                     LKLEQYRRGEEFVAGVAAAGGRDAVKLIWAGPESLPTEAEFLDPRAWLSRVAPHRLAA
                     TA"
     gene            complement(3689..>4505)
                     /gene="murI"
                     /locus_tag="VMZ33_06080"
     CDS             complement(3689..>4505)
                     /gene="murI"
                     /locus_tag="VMZ33_06080"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HUR16832.1"
                     /translation="RAIGFFDSGVGGLTVLREVVRRLPAESTLYLGDNARAPYGPRSD
                     DEVRRFSGECLDELAARDVKAIVVACNTSSAVALPSLRLRYDVPLLGVVRPGATAAAL
                     ATRNRRVGVIGTLATIRSRAYFQAIKEEDPFIEVFEHATPDLVPLVESGRLDGPLVED
                     VIRRALGPFVNGGQDIDTLLLGCTHYPLLGAVIRAVIGPDVTVIDTATATASALAGLL
                     EIHSLGAQSPEAEPHALHVQLTTGDVAQFESIARPLFDDQFANVEPVAVASAV"
BASE COUNT          773 a         1498 c         1489 g          745 t
ORIGIN      
        1 cttgcgcacg attccgagta tgacgagacc gaatacttcg acctcgtcat ggaagcgcgc
       61 gaggccgtct tgcgcaactt cgaggaggag tcgctcatcg aggcgatcgc caccgagctc
      121 gagcgcaaac acgggttcgt ccatgtcgag aacagcctgc gcgccgccgt tcgggtgtcg
      181 ggcgaggaag gcgagactga ggttacgccc gttgttgagc gggcgcacgg catcgaccgg
      241 gaggcggacg acttccggac gatgttggtc gaggtcgaac cagaggaccg cccgtaccgc
      301 aattgagttg ccgttcgatc agacgttgaa tcgaatctcg acgacttccc cgtccttcat
      361 cgggtagtcc ttgccctctg aacgcagttt ggcgttcttg cgggcctcgg cgaacgactt
      421 gagctcgatg aggtcttcgt aggccacgat ctcggctcga atgaagccgc gcgccaagtc
      481 ggtgtggatt gccgccgcgg cgtccacggc ggttgaaccg gccgcgatcg tccaggcgcg
      541 tgtctcgtcc ggtccggccg tgaagaagga gatcaagccc agcagctgat agctcaagcg
      601 gataacacgc tcgagcgacg actcggtgag gcccatctcg tcgcggaaaa cttgcgcgtc
      661 ctcggcatcg agctggccga tctccatctc gacctgcgct gacagcgcct ccacctggga
      721 aaaccggtgt tgatagcgct tggcaaactc ggccgcgatc tcggctgacc gcgcgatatc
      781 ggcctcaccg atgttgagca ggacaagcac cggcttctca gtcaggaacc ggaagccgcg
      841 gatgcgcttc tgttcgtcgt cgtcgagcgt tacgtcgcgg atgggcttcc cctcacggag
      901 ggacggcgca agcttctcca acacgaccag ctcccgctcg ttggactctc gctcggccgg
      961 cgtcccgtgg cgccctgacg tcgacagctt ctcgatgcgc ttctcgatta ctgagagatc
     1021 ggccagcacg aactccaggt cgagctgctc aacgtcgcgc cagggatcga cggagccatc
     1081 ggggtgaggc acggatgggt cctcgaacgc cctgaccacg tgcagcaact cgtcggcggt
     1141 gcgcagccgc gcgagctgat cggccgggat ctccgccggc tcgcgaccgt ccgcggaggt
     1201 cggcggcgcg ggcaggtcca cgtacgtcac gtcggccggg acctcgcgct tgggcttgaa
     1261 gatctcggtc agcctggtta gccgttcgtc ggggaccttg actacgccga cgttgacagt
     1321 catcccaccg aaaccgcccg tttcggcctg acctctggta agcgtgttga agacggtcgt
     1381 cttgcccgat ctggccagcc cgacgatcgc tacctgcact gcatcctggt cgagccctta
     1441 gtagccggcg tttggatcga cgcggttgag caacggctca ccccgacgga agagttcgag
     1501 gttctggcag aacaggtcga tgctgcggtc gagcacgcga ccgctcgacc acgaggtatg
     1561 cggcgtcacg atgatgttgg gcaccgaata gagctcagag tcagccggaa gtggctcctc
     1621 gcgaaatgcg tccaggacag caccaccgag gcgtccctca cggatggcgc ggatcagctc
     1681 gcgatcgtcg acgagcgcgc cgcgcgcgat gttgatcagc cacgcgccag gcttggcgtg
     1741 ggccagaagc tcagcgtcga ctgtgttctc cgtctccggc gtcagcggca gcgcgaggac
     1801 aacgaaatcg ctgcgagcca ttagctcggg cagcccgtcg gcgggcagca gatcgatcaa
     1861 cggcatgatg ccgtcttcgt cgccctcgtc cggcgtcgcg tcgctgaagt ccgcccgacg
     1921 ctttgtcgcc accacctggc cacccatcgt caggacgatc tctgccaccc gtcgcccgat
     1981 cgatcccagt ccaactatcc cgaccgtgac gtccgccatc tccctaccct caagcggctg
     2041 ccacgtgcgt tccttctgaa gctcgaggag cagcggcaac ctgcggctca cggcgaggat
     2101 ccacatcagg acgtactcgg cgatcgggtc ggagaagacg ccccgcgcgt tggtgatggc
     2161 caggccgcgc tccagcgcca gcggggtgag gactcgctcg acaccggcag tcgccgagtg
     2221 gacccacatg agtcgaggtg aacgcaggag cagccgatca aacacggcca tcggcagcgg
     2281 tccacggagc aggacctcca catcgtcaag gtcgctgtcg gcgtgaccct ccatcgacac
     2341 gttgaccagc ctgctgccgg gcgcggctgc agcgatgcgc tccacgtcct gctggcggta
     2401 gcgggctgag aggatgggcg agagtccgat cgcggcgggc actcccccgg ggccggagcc
     2461 gggcccggac agtcgttgtg tctgtggaga cgacgggctc acgcggtggc agccagccga
     2521 tggggagcga cgcggctcag ccacgcacgt gggtccagga actcggcctc tgtcggaagc
     2581 gactccggcc cggcccagat gagcttcacg gcgtcccgac ccccggccgc ggccacgccg
     2641 gcgacgaact cctcgcccct gcggtactgc tcgagcttga gatcgaggcc ggtcagtcgg
     2701 gcgatcaccc ggtccagcgt gcctcgacgt tggtagcgcc gcgcgtcgaa cttctcgcgg
     2761 atcaccgtga cgttgggcag cacttcgctg ccgacctcat ccatgaccca gtcgctgaag
     2821 ccctcgagca ggctcatcac ccgctgcgtt tcctcgaaga gccggcgctg ctccgggccg
     2881 agcagtccaa ccagcgggtt gtcgctggac tcccgcaggc gtcgcagcaa cgtgcccagg
     2941 ccctcggatt gcatgctctt cgcctgatcg aggaccccga cgagctggcg ctccagccgg
     3001 tcgcggaggt acggccgcaa ccaggtattc gcctcgaact cgtaggcatg ggtggtctcg
     3061 tgcaacgcga tccacacgcg gaagtcgtcg agcgacacat cgagggtcgc cgcggtcgcg
     3121 cggatgttct cgtcgacgaa gagcagcttt ccgggttgtg cctcggcaga gagcagggct
     3181 atgtcgtact ggccgagcac gcgcgtgccc agatagccga gcaggaagcc gacttgctgc
     3241 gtcgtcgcga agcggttgat caggcgagcg acgtccgccg caaagccgtg gcctgccgtg
     3301 acactgcgga tgtgcggctc gaggtggcca atcaactgct ggaatgtggc gatgtttgcg
     3361 cgcgcccagc cggcgcgatc cacgacagcg tggcgctcga cgacgcctgg aagggcgaag
     3421 ccgaggcgct gctccaggag tggcaccacg ccgcgcatcg cgcgtgcgta cgcgtgttcg
     3481 gccgtccgca gttgttgctg tgtcagtgca cccggcgctg ccgccagtcg cgcaatcgcg
     3541 atctgctcag ccttgcgcca atcaacgacc gtgtcgccca cctgctgcgc cttgcgcgat
     3601 aggtagcgcg aaccgattgc cgccactccc gctgcaccga tcgccagccc caccccgacc
     3661 aggcggtcgt tccgccgcgc cactgccatt aaacggcgct cgccacagcg acaggctcga
     3721 cattggcgaa ctggtcatcg aagagtggcc gagcgattga ttcgaactga gccacgtcgc
     3781 ccgttgtgag ctggacgtgc agggcgtgag gctcagcctc aggggactgc gcgccaagtg
     3841 agtggatctc gagcagcccc gcaagcgcgc tggcagttgc agtggcggta tcgataacgg
     3901 tcacgtcggg gccgatcacc gctcggatga ccgcaccgag cagcgggtag tgcgtacacc
     3961 cgagcaacag tgtgtcgatg tcctgtccgc cgttcacgaa tggtcccagt gcgcgacgga
     4021 tgacgtcctc aacgaggggg ccgtcgaggc gaccggactc aaccagcggc accagatccg
     4081 gcgtggcgtg ctcaaagacc tcgatgaacg gatcctcctc cttgatcgcc tgaaagtagg
     4141 cgcgcgatcg aatggtcgca agggtgccga tcacgcccac ccggcgattg cgggtcgcga
     4201 gtgcggcagc cgtggcgccg ggacgaacga cgccgagcaa cggcacgtcg tagcgcaagc
     4261 gcagtgacgg caacgcgacg gcgctcgacg tgttgcacgc cacgacgatg gccttgacgt
     4321 cgcgcgcggc gagctcgtcg agacattccc cggagaaccg gcggacctca tcgtcgctgc
     4381 gcggtccgta tggggctcgg gcgttgtcgc ccaggtagag cgtcgattct gcgggcagtc
     4441 gtcggacaac ctcgcgcagg accgtcaggc cacctactcc ggaatcgaag aagccgattg
     4501 cccgc
//
LOCUS       DATLCO010000258         3027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4162499, whole genome shotgun sequence.
ACCESSION   DATLCO010000258 DATLCO010000000
VERSION     DATLCO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4162499"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..2620)
                     /gene="pnp"
                     /locus_tag="VMZ33_06085"
     CDS             complement(29..2620)
                     /gene="pnp"
                     /locus_tag="VMZ33_06085"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HUR16833.1"
                     /translation="MPVTTLSTEIAGRTLTIETGRLAMLAGGSVTVRYGDTMLLGTAN
                     RSEPRPGLDFFPLTIEFEERMYAAGKIPGGFIKREGRSSEGATLAARLTDRPLRPLFP
                     EGYKDDVQVVLTVLSTDQENLPDVVGTIAASAALTISEIPFNGPVAAIRIGRINGEFV
                     INPTVTQLEESELDLIVSGTADAIMMVEAGAKIVTEDVMAEAIMFGHRSLRPIIDLQN
                     QLREQVGKTKRNGYIEPGVDSILEFVKATEQDQSFVVFDVETTSRDVKQGNIVEIGAV
                     RVQGGSIVDRWSTLVNPGMPIIGRQLHGLTDQDVKDSPSPRDAAQQFMTWAGDSLLVG
                     HNIGFDIGFIESALGHEQHIEPGRYLDTLVLAREAYPDTDLKLGDLARFFELEVEPNH
                     RALPDAEATAQLLIRLAGDLPERLNSYRETVSNAIRSLRNGGSKEDVTKALESARKTS
                     RFSKSLSGLLHKKVVRELVLNENIRMDGRDTDTIRPISVEVGLLPRAHGSALFTRGET
                     QALSVATLGPSSDVQRIDTISPEESKRYLHHYNMPPYSTGENKPMRGPGRREIGHGAL
                     AERALIPVLPSHEEFPYVIRVVSECVTSNGSTSMASTCGSTLALMDAGVPIKAPVAGA
                     AMGLVSEPDGRFAVLTDILGKEDAFGDMDFKVTGTADGITAVQMDIKVQGINEQIIRT
                     GLQKAHAARMSILDKMLEVMPSTRTEMSAFAPRIITIKINPEKIRDIIGKGGATIRKI
                     QEDTGTEINVEDDGTVEIAAIDGDKAKAAINMIEGITREIEIGGLYLGKVTRIMGFGA
                     FVEILPGKEGLVRIGELADYHVPTVEDVVSVGDEVMVVVMEVDRQGRVNLSRKAAMQR
                     HLAKEPV"
     gene            complement(2653..2922)
                     /gene="rpsO"
                     /locus_tag="VMZ33_06090"
     CDS             complement(2653..2922)
                     /gene="rpsO"
                     /locus_tag="VMZ33_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HUR16834.1"
                     /translation="MPLAREAKEGIVAEYARSESDTGSAEVQIALLTERIKDLTGHLR
                     SFPKDNHSRRGLLKLVGQRRRMLAYLMRKDNARYRAVIGSLGLRR"
BASE COUNT          506 a          938 c          985 g          598 t
ORIGIN      
        1 gaactgtgtg tgcgggagcg acgggtggct agaccggttc cttcgccagg tgacgctgca
       61 tcgcggcctt gcgggacagg ttgacccggc cctggcgatc gacttccatc acgacgacca
      121 tcacctcgtc gccaacggac acgacatcct cgacggtcgg cacgtggtag tcggcgagct
      181 cgccaatgcg gacgaggcct tccttgcccg gcaggatctc gacgaaggcg ccgaagccca
      241 tgatgcgggt gaccttcccc aggtacaggc cgccgatctc gatctcgcgc gtgatgccct
      301 cgatcatgtt gatcgcggcc ttcgccttgt cgccgtcaat ggcggcaatc tcgaccgtgc
      361 cgtcgtcctc gacgttgatc tcggtgccgg tgtcctcctg gatcttgcgg atcgtggcgc
      421 cgcccttgcc gatgatgtcg cggatcttct ccggattgat cttgatggtg atgatgcgcg
      481 gggcaaaagc gctcatctcg gtacgtgtcg acggcatcac ttcgagcatc ttgtcgagga
      541 tggacatgcg cgccgcgtga gccttctgaa ggccggtgcg aatgatctgc tcgttgatgc
      601 cctggacctt gatgtccatc tggacggcag tgatcccgtc tgctgtcccg gtgaccttga
      661 agtccatgtc accgaaggca tcctccttgc cgaggatgtc agtcaggacg gcaaagcggc
      721 cgtctggctc tgacacgaga cccatcgcgg cgccggcaac cggcgccttg atcggaacgc
      781 cggcgtccat cagggcaagt gttgaaccgc aggtcgaggc catcgacgtg gaaccatttg
      841 aggtcacgca ctcggacacg acgcggatca cgtacgggaa ctcttcgtgg gacgggagaa
      901 ccgggatcag ggcacgctcg gcgagcgcac cgtggccgat ctcacgccgg ccggggccac
      961 gcatcggttt gttctcgccc gtcgaatagg gcggcatgtt gtagtggtgg aggtagcgct
     1021 tcgactcctc cgggctgatc gtgtcgatcc gctggacgtc tgacgacggc ccaagcgttg
     1081 caacgctcag agcctgtgtc tcgccacgcg tgaacagtgc cgagccatgg gcgcgcggca
     1141 ggagcccgac ctcgaccgag atcggccgga tggtgtccgt gtcgcgaccg tccatgcgaa
     1201 tgttctcgtt cagcacgagc tcgcggacga ccttcttgtg gagcagaccc gagaggctct
     1261 tggagaaccg cgacgtcttg cgcgcggact cgagcgcctt ggtgacgtct tccttgctgc
     1321 cgccgttgcg gagcgagcga atcgcgttcg aaacggtctc gcggtagctg ttcaggcgct
     1381 cgggcagatc gcccgccagg cgaatgagga gctgcgcggt cgcttcggcg tccgggagtg
     1441 cacgatggtt gggctcgacc tcgagctcga agaagcgggc cagatcgccc agcttcaggt
     1501 cggtgtcagg gtatgcctca cgggccagta cgagcgtgtc gaggtaacgc cccggctcga
     1561 tgtgctgctc gtggcccagg gccgattcga tgaagccgat gtcaaagcca atgttgtgcc
     1621 cgacaaggag cgagtccccg gcccaggtca tgaactgctg tgcagcgtcc cttggtgacg
     1681 gcgagtcctt gacgtcctgg tcggtgagac cgtggagctg gcggccgatg atcggcatgc
     1741 ccgggttaac gagagtcgac cagcgatcga cgatcgaacc accctgcacg cgcacggccc
     1801 cgatctcgac gatgttgccc tgcttgacgt cgcgactcgt ggtctcaaca tcgaagacga
     1861 cgaagctctg gtcctgctcg gtggccttta cgaactcgag aatcgagtcc acgcccggct
     1921 cgatatagcc attccgcttg gtcttgccaa cctgctcgcg cagctggttc tggaggtcga
     1981 tgatcggacg cagcgagcgg tggccgaaca tgatcgcctc agccatcacg tcctcagtga
     2041 cgatcttggc gccagcctcg accatcatga tcgcgtcggc agtgccggag acgatcaggt
     2101 caagctccga ctcctcaagc tgggtgaccg tcggattgat cacgaactcg ccgttgatgc
     2161 ggccgatgcg gatcgcagcg acggggccat tgaacggaat ctcgctgatg gtcagcgcgg
     2221 cggacgcggc aattgtgccg acgacgtccg gcaggttctc ctggtccgtc gacaggacag
     2281 tcagcacgac ctggacgtcg tccttgtagc cctcgggaaa cagcgggcgc agagggcggt
     2341 ccgtcaggcg ggcggccaga gtggcgccct cagagctgcg gccctcacgc ttgatgaagc
     2401 cgccggggat cttgcccgcg gcgtacatgc gctcctcgaa ctcgatcgtg agcgggaaga
     2461 aatcgagtcc cgggcgtggc tctgagcggt tggccgttcc gagcagcatc gtatcgccgt
     2521 agcgaacggt gacggaacca ccggcgagca tggcgagcct gcccgtttcg atggtgagag
     2581 ttcgaccggc gatctcggtc gacagggttg tgactggcat cagtcctcca ttggcggagg
     2641 acgggttctt gcctagcggc gcagtccgag gctgccgatt actgcgcgat atcgcgcatt
     2701 gtccttgcgc atcagatacg caagcattcg gcgtcgctgc ccgactagct tcagcaggcc
     2761 acgacgcgag tgattgtctt tggggaagga gcgcagatgc ccggtcaagt ccttgatccg
     2821 ctcagtcaag agcgcgatct gaacctcagc cgaacctgtg tcactctccg atcgggcgta
     2881 ctcagccacg atcccttcct tggcttctcg cgccaggggc acccgttcct ccggtatttt
     2941 ccttgcttct attcgcgccg agtctagcag gcgttcggtt tcgcagtaga tttcgtgctc
     3001 gctccgcgtc cgcatccatc tgtttga
//
LOCUS       DATLCO010000259         4679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7681371, whole genome shotgun sequence.
ACCESSION   DATLCO010000259 DATLCO010000000
VERSION     DATLCO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7681371"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VMZ33_06095"
     CDS             complement(<1..385)
                     /locus_tag="VMZ33_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16835.1"
                     /translation="MSRSLLALLLAVFATIGGASAAMAHFGSEAAIIVPADYLLPGQQ
                     FDAVAVDLAPGSVVSFEMRRDALVVALGSAPSPGDGHFTLTMAVPADFPTGYAELVGT
                     SSDGTSAMTWVLVGERNPATPAAPPG"
     gene            complement(455..2278)
                     /locus_tag="VMZ33_06100"
     CDS             complement(455..2278)
                     /locus_tag="VMZ33_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HUR16836.1"
                     /translation="MPKRPVTLAVGASCHISGGVLDVINSRSASPRARRPILGLLALI
                     AALVVLLPQVVLAAKTAPAPVTIQILNVSDWHGNLDPVAGLGGAWNLSARWQQDRTAF
                     PSLTLTAGDDFGATPALSSFFNEVPAVLAERMMGIQVGALGNHNFDRGVTHLQSMVNL
                     AGAPTSASTPGSPYSYVAANLDLNGQLAGVDPYRIFEVGGVRVGVIGVTNEEAPSVVL
                     PGAFGDIVITDSVAAANKVAKQIRMFTDVTLVITHKGVRGFTAGQPFGELIDFANALD
                     PENVDVVFGDHTDVQYSGTHNGILVHENRSYGIGYAKTLLSVQLGNGNGSKRSEVISK
                     SVSFVSPGPSGALSSGNTSCGVLTFCDQAIVNMLVPYRQQLAALLDGTVGTATGTFVR
                     GGNIERRQEVPIGDLIADGMRWKYGTQLALMNGGGIRSALPSSYAPANTSLRRPVAGY
                     ACGVACPAPWDLVLGDVFTVLPFSNIITTRTVTGAQLWAALENGVSAINSVTGLGTDG
                     RFPQISGFKFTFDYGNAVGSRVLSVALSDGTPIPNNASSTYTMALPNFVNLGGDGYTV
                     FNDGTGTTQALDALVMKDYMAVAGPTFDPTSYPLDRIIKQP"
     gene            2386..4035
                     /locus_tag="VMZ33_06105"
     CDS             2386..4035
                     /locus_tag="VMZ33_06105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HUR16837.1"
                     /translation="MSLHPAVEDRLARLADDQRAAATAPPGPVLCVAPAGSGKTTTLV
                     ARICWLVAGGTDPEQITAVTFNKRAADELEARLKEALAPLGDTGPTPRVRTFHALGRE
                     ILVDAGVTVSNLMDRAEVLRRLNGKPLRSSTLRRFDDAISRLKLDDMVDADEVRQIVA
                     AQGELPNDPAICSAFVAYEDWLKNNGALDFDDLVRLCLRRLRADPVLLARWRERCRVM
                     LVDEVQDVDRTQLELALLLAGEQPNVFLVGDDDQTIYAWRLADVRRVLELAGALSGLR
                     RVDLVTNYRCPRPVVQRAVRLVERNQERFAKMIQAGPSAAGDLVLCPDPGDDVARARR
                     LLTTWFKSGTGGHAVLSRTNAELAPFAAVAIELGIPYRAADDGLALDDARLNDVLDAL
                     PVDQPPAVALAGVQWLAAWALPYRTADDFRAAVGHARDQRRKLRREDAELVLATAHGT
                     KGLEFDHVVCIGNDEGVFPSRRTLEEAENPTRALEEERRLAYVAWTRARKSLTLVYDP
                     SAPSVFMRDAFSADELQFQRVSNGSTARRMLPRSSAAGSMG"
     gene            complement(3963..>4679)
                     /locus_tag="VMZ33_06110"
     CDS             complement(3963..>4679)
                     /locus_tag="VMZ33_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16838.1"
                     /translation="PAASSTSTRPSTAALASDATAGPTAAGSVAASPQASTPPPVTPD
                     TTPEQLPTQAATSSPASSGADLAALLQSLAPALATFGASSPVLATGEEVLAQLQASLD
                     EMSAMFPGHSNLQIMQDEIDAYTENGLVERASFSVDTTPPAGMLIYRYESEAGAAADF
                     AMRNRDCDEINVPAPAIVDIAAKSCNTVDSHNDVYLNAHRGDLVVILQVRDLPEDQPI
                     EPAAELLGNILRAVDPLLTR"
BASE COUNT          873 a         1438 c         1570 g          798 t
ORIGIN      
        1 tgcccggtgg cgcggcgggc gtagccggat tgcgctcacc cacgagcacc cacgtcatcg
       61 cgctcgtccc gtcggaggat gtgccaacga gctccgcata accggtaggg aagtcggcgg
      121 gcaccgccat cgtcaaggtg aaatgcccat ctccgggcga cggcgccgag cccagagcga
      181 cgactagagc gtcacgacgc atctcgaagc tgaccacgga gcccggcgcc agatcgactg
      241 ctacggcgtc gaattgctgg cccgggagga ggtagtcggc cggaacgatg atcgcagcct
      301 ctgacccgaa atgagccata gccgcagatg cgccgccgat ggttgcaaag accgccagga
      361 gcagggcgag cagtgaccgc gacatgaacg gcaggataaa agaagaggcc gggcatgacg
      421 cccggcctct ctctaagaac tttctcagac tgtgctacgg ctgcttgatg atccggtcga
      481 gcggataact ggtcggatcg aacgtgggcc cggccacagc catgtagtcc ttcatgacga
      541 gggcgtcgag agcctgggtg gtcccggtgc cgtcgttgaa cacggtgtac ccgtcgccgc
      601 cgaggttgac gaagttcggg agcgccatgg tgtacgtgct cgatgcgttg ttcgggatcg
      661 gggtcccatc ggacaatgca acggagagca cacgcgatcc aacagcattg ccgtagtcaa
      721 aggtgaactt aaacccggag atctgcggga accggccgtc ggtcccgaga ccggtcacgc
      781 tgttgatcgc gcttacgccg ttctccagcg ctgcccagag ctgggcccca gtgaccgtgc
      841 gggtagtgat gatgttgctg aaaggcagca cggtgaacac gtcgcccaag acaaggtccc
      901 atggagccgg gcaggccacg ccgcatgcgt atccggcaac cggtcgacgg agcgaggtgt
      961 tggccggcgc gtacgaggag ggcagtgcgg acctgatgcc gcccccgttc atgagcgcaa
     1021 gctgcgtccc gtacttccag cgcatgccgt cagcgatgag atcgccgatc ggcacttcct
     1081 gacgtcgctc gatgtttccg ccgcgcacga atgtgcccgt cgcagttccc acggtcccgt
     1141 caagcaaggc ggcgagctgc tgtcggtacg gaacgagcat gttgacgatc gcctgatcgc
     1201 agaatgtcag cacgccacac gacgtgttgc ccgagctgag cgcgccagac ggtcccggcg
     1261 agacgaacga aacggacttg ctgattacct cactgcgctt ggagccgttg ccgttcccaa
     1321 gctgaacgct gagaagggtc ttggcataac cgatgccata gctgcggttc tcgtgcacga
     1381 ggatcccgtt gtgcgtgccg gagtactgga cgtctgtgtg gtcgccaaag acgacgtcga
     1441 cgttctccgg gtccaacgcg tttgcaaagt caatgagctc gccgaacggc tggcccgcag
     1501 taaaaccgcg gacgccctta tgcgttatga caagcgtgac atccgtgaac atgcggattt
     1561 gcttggcgac tttgttcgcg gccgcaaccg agtccgtgat gacgatgtcg ccgaaggctc
     1621 cgggcagaac tacgctcggc gcctcttcat tggtgacgcc gatcacgccg acccggacac
     1681 caccaacttc gaagatccga tacggatcga cgccggctag ttgaccattg agatcgaggt
     1741 tggcggcgac gtatgaatag gggcttcctg gcgtgctcgc gcttgtgggt gcgccggcca
     1801 agttgaccat cgactggaga tgagtgacgc cgcggtcgaa gttgtggttg ccaagcgcgc
     1861 cgacctggat gcccatcatt cgctcggcga ggaccgccgg gacctcgttg aagaagctcg
     1921 agagcgccgg cgtcgcgccg aagtcatcgc ccgcggtcaa cgtgagcgaa gggaatgcag
     1981 tgcgatcctg ctgccacctg gctgataggt tccaagcccc gccgagcccg gcgaccgggt
     2041 cgaggttgcc gtgccagtcg gacacattaa ggatctggat agtcaccggc gcgggggcag
     2101 ttttcgccgc gaggaccacc tgtggcagaa ggacgacgag cgccgcgatg agggctaaca
     2161 gcccgagaat aggacggcgt gcgcgtggcg acgccgaccg tgagttgatc acgtcgagaa
     2221 cccctccaga gatgtgacac gacgcgccca cggctaaggt gacggggcgc ttcggcatgc
     2281 caatgctatc gcccggtagg gacctgcgaa ctacgaactt cgcagtattc gtccacataa
     2341 gcgaggagta agcagccgat acgcgaaccg ctcgaccatg agccggtgtc acttcacccg
     2401 gctgtggaag accgactggc gcggctcgcc gacgaccagc gagcggctgc gaccgcaccg
     2461 ccgggaccgg tgttgtgcgt tgcgcctgct ggctccggga agacaacgac cctggtggcg
     2521 cggatctgct ggctcgtcgc cggaggtacg gaccccgagc agatcacggc ggtcacgttc
     2581 aacaaacggg cggccgatga gctggaggcc cgcctgaagg aggcgctggc gccgctaggc
     2641 gacacgggac caacgccacg ggtgcgcacg ttccacgcgc tgggacgcga gatcctcgtc
     2701 gacgccggcg tcacagtctc aaatctgatg gatcgcgcgg aggtcctgcg gcgactcaac
     2761 ggcaagccgc tcagaagcag cacgttgagg cgcttcgacg acgccatctc aaggttgaag
     2821 ctcgacgaca tggtcgatgc cgacgaagtg cgacagatcg tcgccgcgca gggcgagctg
     2881 cccaacgatc ctgcgatctg ttcagcgttc gtcgcctacg aggactggct gaagaacaac
     2941 ggagctctgg attttgacga ccttgttcgc ctttgcctca gacgtctacg agcggaccct
     3001 gtcctcctcg cacgttggcg cgaacggtgc cgcgtgatgc tcgtggacga ggtccaggac
     3061 gtggaccgga cgcagctcga actggcgttg ttgctcgctg gagagcaacc aaatgtcttt
     3121 ctcgtcggcg atgacgacca gacgatctac gcctggcgtc tcgccgatgt ccgccgagtg
     3181 ctggaactgg cgggcgcgtt gtcgggcttg cggcgcgtcg acctcgtaac caactaccgc
     3241 tgcccgcgac ctgtcgtcca gcgagccgtg aggctcgtcg aacgaaacca ggaacggttt
     3301 gcaaagatga tccaggcggg accgagcgca gccggtgacc tggtcttgtg ccctgacccg
     3361 ggggacgacg tcgctcgggc gcggcgattg ttgacaacct ggttcaagtc cggcaccggt
     3421 ggccacgcgg tcctttcccg aacgaacgcc gagctggccc cattcgcggc tgtcgccatt
     3481 gagctcggga tcccctaccg cgcagcggac gatgggctgg cgctcgacga cgccaggctg
     3541 aacgatgttc tcgacgcgtt gccagttgac cagccgccgg cggtcgcgct cgccggcgtc
     3601 caatggctcg cggcgtgggc gctcccctac cgaactgccg acgactttcg cgcggccgtg
     3661 ggtcacgctc gcgaccaacg ccggaagcta cgtcgcgagg acgcggaact cgtcctggca
     3721 acggcacacg ggacgaaggg tctcgagttc gatcacgtgg tctgcatcgg caacgatgag
     3781 ggcgtgtttc cgagccgccg aacgctcgag gaggcagaga acccgacgcg cgcgctggag
     3841 gaagagcggc gcttggccta tgtagcgtgg acgcgcgcca ggaagtcgct caccctggtc
     3901 tatgacccga gcgcgccttc agtgttcatg agggacgcgt tcagcgcgga cgaactccaa
     3961 tttcagcgcg tcagcaacgg atcgacagcg cggaggatgt tgcccagaag ctcggcagcg
     4021 ggctcgatgg gctgatcctc cggcagatcg cggacctgca gaatgacgac cagatcgcct
     4081 ctatgcgcgt tcagatacac atcgttgtga gagtccaccg tgttgcagct cttcgctgcg
     4141 atgtcgacga tcgcaggtgc cggtacgttg atctcatcgc aatccctgtt gcgcatcgcg
     4201 aaatcggctg cagcgccggc ctcggactcg tatcgataga tgagcatccc cgccggaggc
     4261 gttgtgtcga ctgagaatga agcacgctcg accagcccgt tttcggtgta agcgtcgatc
     4321 tcatcctgca tgatctgaag gttcgaatgg ccggggaaca tcgcgctcat ttcgtcgaga
     4381 cttgcctgca gctgggccag gacttcttcg ccggtcgcga gcacgggcga gctggcaccg
     4441 aaggtggcca gcgcaggtgc cagggattga agaagagcgg cgaggtcagc gcccgatgac
     4501 gccggactac tcgtcgcggc ctgagtgggt agttgctcgg gggtcgtgtc tggcgttacc
     4561 ggtgggggcg tagacgcctg tggagaggcc gcgaccgacc cagccgcagt gggcccagcg
     4621 gtcgcatctg acgcgagcgc agctgtcgat ggccgagtgc tcgtgcttga tgcagcggg
//
LOCUS       DATLCO010000260         4479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9924280, whole genome shotgun sequence.
ACCESSION   DATLCO010000260 DATLCO010000000
VERSION     DATLCO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9924280"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /gene="folP"
                     /locus_tag="VMZ33_06115"
     CDS             <1..555
                     /gene="folP"
                     /locus_tag="VMZ33_06115"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HUR16839.1"
                     /translation="STKADVAAAALEAGADVINDIWGTGASDAMAGLAAERNAPMIVM
                     HNRMEPRYRNVVAEVLGDLQRALDRALARGVDWDNLIVDPGIGFGKTAEQNLAILRDL
                     SLITALGRPVLLGTSRKSTIGKVLDLAADQRLEGTLATTALGVAAGADIVRVHDVRQN
                     VRAARMADAIVRGGWVDPTREPQR"
     gene            552..914
                     /gene="folB"
                     /locus_tag="VMZ33_06120"
     CDS             552..914
                     /gene="folB"
                     /locus_tag="VMZ33_06120"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HUR16840.1"
                     /translation="MTDRIVLHHMEFEGFHGVDDEERSLPQVIEVDVELEVDLRTAGA
                     TDDLEQTVNYSQVFKRCRTIVEEQSFHLLEAMAHTIASDLIATFAPIKSLVVRVRKPG
                     VPIEGVLDFAGVEIERSR"
     gene            complement(988..2325)
                     /locus_tag="VMZ33_06125"
     CDS             complement(988..2325)
                     /locus_tag="VMZ33_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUR16841.1"
                     /translation="MDEETTDSYNRYEGYDPTAGSYSPPAQTPQPWTGSSYQQPTPQH
                     WLEPLPGQERRRSPRRRGRAGLTAVMVVLVALIAGGAGSLATYGALLYGGALNNGQQV
                     SAVPTPSPQTQPQPSPQTVILDEQSAITAAAESVSPAVVTITVSGEATDPFSLPSTGV
                     GSGIIYDANGWILTNRHVVVDATSVSVELHDGSEVTGTVYGVDTLTDLAIVKIDAPNL
                     VAAPVGDSSQLKPGQTAVVIGSALGTFTNSVTSGVVSALGRQLVVTDQVTGERRRLRN
                     LIQTDAAINPGNSGGPLIDAAGNVVGVSTAYAEQAQGIFFAIPINIAKPIMRQAVAGE
                     SLSRPYIGIIYVPVDRNVEAENDLPIDYGAWIAPDTSGGGEPIVPGSPADSAGLRAGD
                     IVTAIDGRRIDASLDLDDILTNYVPGDRVTLDILREGTTLQLGVTLAERPANI"
     gene            complement(2355..3905)
                     /locus_tag="VMZ33_06130"
     CDS             complement(2355..3905)
                     /locus_tag="VMZ33_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUR16842.1"
                     /translation="MRRLIPRFATGLRARLVLAFLAVVAIALALVFATLPRLLDGYFA
                     QQSQTDLDRRAGVAKLELAREITRYQATGGDAPRPILVPTEPLSASDGLRALLGTDSR
                     FASDLAQIVQADVTITIAPDPEHTSEVAYQSQQQLPDSFGEVGQQRESISSDPGAGQI
                     EIADLFWTQSGAAPQRVVVVTLSNPFTFRAQTLQQIIEVMTVAAALAMLAAVIASVLI
                     ADRLSEPVRRLKSAATELADGRLETRVMPRSASPEMQELGEAFNTMAERLQHSIEFIR
                     SDRDRSRDFLADVSHELRTPIAALRTFNELFSDEPTMDEVTRKEFVEQSRRQIERLDW
                     LATNLLELSKLESGLVLLDLRPDDLRAAVENAVAQQLPNAERKNISLRMELPDEPVRQ
                     RHDPQRVGQVLTNLIGNAIKFTPIDGRVDVDLEQTKDGAELRVSDSGIGIDPRELPMV
                     FDRFYRGANSHESRASGSGLGLSIVRQIVEMHNGRVAITSTPGKGTQVSVALPNDVAV
                     SSQATVRT"
     gene            complement(3930..>4479)
                     /locus_tag="VMZ33_06135"
     CDS             complement(3930..>4479)
                     /locus_tag="VMZ33_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16843.1"
                     /translation="ILDVRLPGMDGVEMLRRVRGAGNKAPVLFLTARDEEMDKVIGLE
                     LGADDYLTKPFGLRELMSRIKALLRRAYGDLADAAGGRVIRHHDLLIDLERRRVQRGT
                     RRISLTATEFEILRHLASKPGRVFTRRELLELVRDYEALDQDEKTINVHVSHLREKLE
                     DDPADPAFILTLRGAGYAFAER"
BASE COUNT          723 a         1416 c         1567 g          773 t
ORIGIN      
        1 tccaccaaag ccgatgttgc ggcggcggcc ctcgaagccg gcgctgacgt catcaacgac
       61 atctggggca cgggtgcttc agacgccatg gccggcctcg ccgcggagcg caacgcgccc
      121 atgatcgtga tgcacaaccg catggagccg cgctaccgca acgtggttgc ggaggtactg
      181 ggcgatctcc agcgcgccct cgatcgcgcc ttggcacggg gagtggactg ggacaacctg
      241 atcgtcgacc ccggcatcgg cttcggcaag accgctgagc agaacctcgc catcctgcgc
      301 gatctatcgc tcatcaccgc gttgggccga ccggttttgc tgggcacgag ccgcaagtcg
      361 acgatcggca aggtcctcga cctggcggcg gatcaacgcc tggaaggaac tctggccacg
      421 accgctctcg gagtggccgc cggcgctgac atcgtgcgtg tccacgacgt ccggcagaac
      481 gtccgtgccg cgcgcatggc cgacgcaatc gtgcgcggcg gctgggttga cccaacgcgc
      541 gagccgcagc gatgaccgac cgcatcgttc tccaccacat ggaatttgag ggcttccacg
      601 gcgtcgacga cgaggagcgg agcctgccac aggtgatcga ggtcgatgtc gagctggagg
      661 tcgatctccg cacggccggc gcgacggacg acctcgagca gaccgtcaac tacagccagg
      721 ttttcaagcg ctgccggacg atcgtcgagg agcagagctt ccatctgctg gaagccatgg
      781 cccacacgat cgcaagcgac ctgatagcga ctttcgcgcc gatcaaatcg ctggtcgtcc
      841 gagtccggaa gcccggagtc cctatcgagg gcgtgctcga cttcgccgga gtggagatcg
      901 agcgctcgcg ctaagggctc acgcccggcg tggaggattg gggctcacgc ccggcgtgga
      961 gggttggggc tcacgcccga caaaggatca gatattcgcc gggcgttcgg ccagggtgac
     1021 gccgagctgt agtgtcgtgc cctcgcgcag gatgtcgagc gtcactcggt ctccggggac
     1081 gtagttcgtc aggatgtcgt cgagatcgag gctggcgtcg attcgtcggc cgtcgatcgc
     1141 cgtgacgatg tcgccggcac gcaagccggc gctgtcggcc ggactaccgg ggacgatggg
     1201 ctcgccgccg ccgctcgtgt cgggcgcgat ccaggcgccg taatcgatcg gcaggtcgtt
     1261 ctctgcctcg acgttgcgat cgaccgggac gtagatgatg ccgatatacg gccggctgag
     1321 cgactcgccc gcgaccgcct ggcgcatgat cggcttggcg atgttgatcg ggatggcgaa
     1381 gaagatcccc tgcgcttgct ccgcgtaggc ggtgctcaca ccgacgacgt tgcccgcggc
     1441 gtcgatcagc ggaccgccgg aattgccggg gttgatggcg gcgtcggtct ggatgaggtt
     1501 gcgcagtcga cggcgctcgc cggtgacctg atcggtcacg acgagctggc ggccaagcgc
     1561 cgatacaacg ccggacgtga cgctgttcgt gaacgtcccg agggcgctgc cgatgaccac
     1621 agccgtctgg ccgggcttga gctggctcga atcgccgacc ggcgcggcaa cgagattcgg
     1681 ggcgtcgatc ttgacgatcg cgaggtccgt cagcgtatcg acgccgtaga ccgtgccggt
     1741 cacttcgcta ccgtcgtgaa gctcgaccga aacgctggtc gcgtccacga ccacgtggcg
     1801 gttcgtcagg atccagccgt tggcgtcgta aatgattccc gacccgacgc ccgtcgacgg
     1861 gaggctgaac ggatctgtcg cctcgcccga aacggtgatc gtgaccacgg ctggactcac
     1921 ggattcagcg gcagcggtga tggccgactg ctcgtcgagg atgactgtct gcggggaggg
     1981 ctgcggctgg gtctggggac tcggtgtcgg gacggctgag acctgttggc cgttgttcag
     2041 cgcgccgccg tacagcagcg cgccatatgt cgcgagtgat ccggctccac cggcgatgag
     2101 cgccaccaag acgaccatta ccgccgtcag gccggcgcga ccgcgcctac gcggcgatcg
     2161 gcggcgctcc tgaccgggga gtggctcgag ccagtgctgc ggcgtcggtt gctggtatga
     2221 cgaacccgtc cagggctggg gcgtctgcgc tggcgggctg tagctgcctg cggtggggtc
     2281 gtagccctcg tatcggttgt atgagtcggt cgtctcttcg tccacggcta gtcgaagggt
     2341 aggccgaacc gcgttcaggt gcggacagtg gcctgtgaag aaacggctac gtcgttgggg
     2401 agtgccaccg agacctgcgt tcccttgccc ggtgtgctgg tgatcgcgac ccggccgtta
     2461 tgcatctcga cgatctggcg aacgatcgac aggcccaggc ctgagccaga ggcgcgcgac
     2521 tcgtgcgagt tcgcgccgcg gtagaaacga tcgaagacca tcgggagctc gcgcgggtcg
     2581 atgccgatgc ccgagtccga cacgcgcagc tccgcgccgt ccttcgtctg ctcgagatcc
     2641 acgtcgacgc ggccgtcgat cggggtgaac ttgatggcgt tgccgatgag gttggtcaac
     2701 acctggccca cgcgctgtgg atcgtgccgc tgacgtacgg gctcgtcggg cagctccatt
     2761 cgcaggctga tgttcttgcg ctcggcgttg gggagctgct gcgcgacggc attctcgacc
     2821 gctgcccgga gatcgtcggg ccgaagatcg agcagcacca ggcccgactc aagcttggac
     2881 agctccagga ggttggtcgc cagccaatcc agccgctcga tctgccggcg gctctgctca
     2941 acaaactcct tgcgcgtgac ctcgtccatg gtcggctcgt cggagaagag ctcgttgaac
     3001 gttcgaagcg ccgcgatggg cgtgcgcagc tcgtgcgaga cgtcggccag gaagtcgcgg
     3061 ctgcggtcgc ggtcgctccg gatgaactcg atggaatgct ggagccgctc agccatggtg
     3121 ttgaaggcct cgccgagctc ctgcatctcc ggcgacgcgc tgcgcggcat cacgcgcgtt
     3181 tcaagccggc catcggccag ctcagtggcg gccgacttca accgtcgtac cggttccgac
     3241 agccgatccg ctatgagcac agacgcgata accgcggcca gcatcgccag ggcggcggcc
     3301 accgtcataa cctcgatgat ctgctgaagg gtctgggctc tgaacgtgaa tgggttggac
     3361 aacgtcacga cgacgaccct ttgcggggcc gcaccggact gggtccagaa cagatccgcg
     3421 atctctatct ggcccgcgcc tgggtcagac gagatcgatt cacgttgctg gccgacctcc
     3481 ccgaagctgt ccggcagctg ctgttgtgat tgatatgcga cctcgctcgt gtgctcgggg
     3541 tcgggcgcaa tcgtgatcgt cacgtcggcc tgcacgattt gcgcgaggtc ggaggcaaac
     3601 ctgctgtcgg tgccaaggag cgcccggagg ccgtcggatg cgctaagtgg ctcagtcggg
     3661 acgagaattg ggcgcggcgc atctccgccc gtcgcttgat accgggtgat ctcccgggcg
     3721 agctcaagct ttgctacgcc cgctcggcgg tcgagatccg tctgggactg ctgcgcgaag
     3781 tagccgtcca ggagtcgtgg cagggtcgcg aacacgaggg cgagggcgat tgccacgacc
     3841 gctagaaagg caaggacgag tcgcgctcga aggccagtgg cgaagcgggg tatcaagcgg
     3901 cgcatcgagg cgctctgggg tgtctccggc tagcgctcag cgaaggcgta gccagcgccg
     3961 cgcaaggtca ggatgaatgc cggatcggcc gggtcgtctt cgagcttctc gcgcaggtgg
     4021 ctgacgtgga cattgatcgt cttctcgtcc tggtcgagcg cctcgtagtc gcgcaccagc
     4081 tcgagcagtt cacgccgcgt gaagacgcgg cccggcttcg acgcgaggtg acgcaggatc
     4141 tcgaactcgg ttgcggtgag gctgatccgt cgggtgccgc gctgtacccg gcgccgctcg
     4201 aggtcgatga gcaggtcgtg gtggcggatc acgcgcccgc cggcggcgtc ggcgaggtcg
     4261 ccgtaggcac gccgcaacaa ggccttgata cggctcatca gctcgcgcag gccgaacggt
     4321 ttggtcaggt aatcgtcggc gcccagctcc aggccgatga ccttgtccat ctcctcgtcg
     4381 cgggcggtga gaaacaggac cggcgccttg ttgccggcgc cacgaacgcg gcgcagcatc
     4441 tcgaccccgt ccatgcctgg caagcggacg tcgaggatg
//
LOCUS       DATLCO010000261         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9925002, whole genome shotgun sequence.
ACCESSION   DATLCO010000261 DATLCO010000000
VERSION     DATLCO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9925002"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="VMZ33_06140"
     CDS             <1..1339
                     /locus_tag="VMZ33_06140"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HUR16844.1"
                     /translation="ILAPVMVVSAAHDIGIDGVLDAMVRYFPSPDEENPAKALDGAGK
                     EVEVAPDANGPLLARVFKTTADPFVGRLTYFRVYSGKIGSHDHIWNANRGEEERIGQV
                     LRVKGKDTEPVGSIGAGEIGAVAKLVHTHTGETLSSREKPFRLPDIEFPRASLGVAIE
                     PETKADLDKMGAALGRLVEEDPGVRLEREVETGEQLLWAQGESHVAVIVERLKRKFGA
                     AVLTRQPRVPYRETIRSRTQVEGRHKKQTGGRGQFGHVWLEIEPNPESGVQFATRIVG
                     GAVPRQFWAGVEKGVHDIAQKGPIAGYPVIDFKATLYDGSFHTVDSDELSFRMAGQLA
                     TRKGIQDASPALLEPIMDVKVRVPEEYMGDVNRDLNTRRGHVLGMDSSDGMQVVHAQV
                     PQSELFTYATELRSLTGGRGMFSATLDHYQEMPAHVAQKVIEEHKKDEAASGH"
     gene            complement(1568..2092)
                     /locus_tag="VMZ33_06145"
     CDS             complement(1568..2092)
                     /locus_tag="VMZ33_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16845.1"
                     /translation="MTRYIPRFAAIIVALAIMVAATGVVLAATSAVTGNAQKQIKVVR
                     ENTTTASATSNSTTYSDIPGATVSMTVPSSTNALLVARFQSHGSVIQTSGCIVRILVG
                     STTMEPNGNYPFMGQANVSESVAGSGAIERSLAVGPGTYTVKAQLRIATANSVNSQCQ
                     LSGWHFAVERHARS"
     gene            complement(2157..2459)
                     /locus_tag="VMZ33_06150"
     CDS             complement(2157..2459)
                     /locus_tag="VMZ33_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16846.1"
                     /translation="MAPSTDQERARHFLVELTRPAAGWALLADVTARARSAASAVRGL
                     GAQVSLVRSVYAPESDSLFLVYMAESEDAVWVALRGAELSPLGVSTAIVADDRGRR"
     gene            complement(2544..>2835)
                     /locus_tag="VMZ33_06155"
     CDS             complement(2544..>2835)
                     /locus_tag="VMZ33_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR16847.1"
                     /translation="AEALEVFDNLGAALWAEAAAAELARISGRTPASGSLTDAETRVA
                     SLVVEGLSNAEIAGRLFVSVRTVEAHLSRIYEKLGARSRVDVVRWASSRNDS"
BASE COUNT          510 a          900 c          942 g          483 t
ORIGIN      
        1 aatcctcgcg ccggtcatgg tggtgtcggc cgcgcacgac ataggcatcg acggcgtcct
       61 cgacgcgatg gtgcggtact tcccctcgcc cgacgaggag aacccggcca aggctctcga
      121 cggcgccggc aaggaagtcg aggtggcgcc cgacgccaac ggtccgctac ttgcccgcgt
      181 cttcaagacg actgccgacc cgtttgtcgg ccggctgacc tacttccgtg tctacagcgg
      241 caagatcggc tcgcacgacc acatctggaa cgcgaaccga ggcgaggaag agcgcatcgg
      301 ccaggtactg cgtgtcaagg gcaaggacac tgagcctgtg gggtcgatag gcgccggcga
      361 gatcggcgcc gtggcaaagc tcgtgcacac gcacacgggc gagaccctgt cgtcgcgcga
      421 aaagccgttc agactgcccg acatcgagtt cccgcgcgcg tccctgggcg tggcgatcga
      481 gcccgaaacg aaggccgacc tcgacaagat gggcgccgct ctcggcaggc tcgtcgagga
      541 ggatcccggc gtacgccttg agcgcgaggt cgaaacgggc gagcaactgc tatgggcgca
      601 gggcgagtcg cacgttgccg tcatcgtcga acgcctgaag cgcaagttcg gcgcagccgt
      661 gctgacccgc cagccgcgcg tgccgtaccg cgagacgatc cgctccagga cgcaggttga
      721 aggccggcac aaaaagcaga ccggcgggcg cggccagttc ggccacgtct ggctggagat
      781 cgagccgaac cctgaatcgg gagtccaatt cgccacgcga atcgtcggcg gcgccgtgcc
      841 gcgtcagttc tgggcgggcg ttgagaaggg cgtccacgac atcgctcaaa agggtccgat
      901 cgccggctac ccggtaatcg acttcaaggc aactctgtac gacgggtcgt tccacacggt
      961 ggactcggat gagctctcgt tccgcatggc cggccaactg gcgacgcgca agggcatcca
     1021 ggacgcctca ccggcgctgc tcgagccgat catggacgtc aaggtccgcg tgcctgagga
     1081 gtacatgggc gacgtgaacc gtgacctcaa cacgcgccgc ggccacgttc tcgggatgga
     1141 ctcttccgac ggcatgcaag tcgtacatgc ccaggtgccg cagtcggagc tgttcacgta
     1201 cgccacggag ctgcgctcgc tgactggcgg gcggggcatg ttcagcgcca ctctcgatca
     1261 ctaccaggag atgcccgccc acgtggcaca gaaggtgatc gaggagcaca agaaggacga
     1321 ggccgcctcc gggcattgac cgttgcccgg ccggtggacg cgtttcgtgt cgacgagaca
     1381 ccacttgacg ccaattcggt tcgatgatgc ctgttggtgc cccttgggcg gcgagagggc
     1441 cgcgcagctc cctctctacc gaacctgact agccggcttc gctcgcgaaa ctcctgtcag
     1501 gttcaaggcc agcgcaaaag gcccgtggga tgtgcccacg ggcccagtcg acgatctgga
     1561 gcgctcgcta cgagcgtgcg tgccgctcca ctgcgaaatg ccatccgctc aactggcatt
     1621 gggagttcac gctgttggcc gtggcgatgc gcagctgcgc cttgaccgtg tacgtgccgg
     1681 ggccgacggc gagtgagcgc tcgatggcac cggatccggc aactgactcg gaaacgttgg
     1741 cctggcccat gaacgggtaa ttgccgttcg gttccatggt cgtggaacct accaggatcc
     1801 ggacgatgca gccgctcgtc tggatcacgg atccgtgact ttggaagcgg gccacgagca
     1861 gcgcattggt gcttgagggc acggtcatcg agaccgtggc ccccgggatg tccgagtatg
     1921 tggttgagtt cgacgtagcg ctggccgtgg tcgtgttttc gcgcacgacc ttgatctgct
     1981 tctgcgcgtt tccggtaaca gccgatgtcg ctgcgagaac gacgccagtc gctgccacca
     2041 tgattgcgag agcaacgatg atcgctgcga agcgcgggat gtatctggtc atatgagtat
     2101 ttctcctggt ctggataggg tttctgtggg caattgctgg ccttggagaa gtcacttcat
     2161 cggcgacctc tgtcgtcagc gacgatcgcc gtagagacac ccagcgggct cagctcggcg
     2221 ccacgaagag ccacccatac ggcatcttct gactccgcca tatagacaag gaagaggctg
     2281 tctgactcgg gcgcatacac ggagcgcacc agggagacct gcgcgcccag gccgcgcacc
     2341 gcgctggcag ctgagcgggc gcgggcagtt acgtcggcga gcagtgccca gcccgcggcg
     2401 ggccgcgtaa gctcgactaa gaagtgtcgg gctcgttcct gatcggttga aggggccatg
     2461 aacggacgct agtcacggcg cgacgtcgtt catatcgtgg aaaaccgttc aacttgaccc
     2521 gcccttggcc ctttttagac ccgttaggag tcgttgcgtg agctcgccca gcgcacgacg
     2581 tccacgcgcg agcgagcgcc gagcttctcg tagattcggg acaaatgcgc ttcgactgtc
     2641 cggacgctga cgaacaggcg gcctgcgatc tcagcgttac tgaggccctc gaccacgagg
     2701 ctcgccactc gtgtttcggc gtcggtcagc gacccagagg ccggtgttcg gccgctgatg
     2761 cgcgccagtt cggcggcggc cgcctccgcc cagagcgccg cgccgaggtt gtcgaagacc
     2821 tcgagcgctt cagca
//
LOCUS       DATLCO010000262         3780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9406422, whole genome shotgun sequence.
ACCESSION   DATLCO010000262 DATLCO010000000
VERSION     DATLCO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9406422"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VMZ33_06160"
     CDS             <1..1170
                     /locus_tag="VMZ33_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR16848.1"
                     /translation="GGDAGVDAFDLRPMVLMPGLIDLHAHLPQYPNAGLGGGLDLLTW
                     LERYIFPLERGFDRAAAERLAPVVFRAFAAAGTTTAVLYGAVYEHSLHACFEAAEAHG
                     IRAVIGKVMMDRLRYDGKVGDREVLDVSLRQSQNLIDRWHGAADGRLQYVVTPRFAVS
                     CSREMLRESADLAKSSGAYWQTHLSEDNHELDEVRELFAEAADYTDVYDRAGGLGPRS
                     IFGHAIHLSEREINRLAETSSKIAHCPSSNLFLASGAMPLSRYIEAGLVVGLGSDVAG
                     GPDISLFSVMRAGAYTQSGLRTMLGETRPQLTPVEWLRMASLDGARALGLEDTIGSLE
                     VGKEADFICVDPSVTAPVAGDGDRDDPAELMSQLIYRSRPDMVKGAWVRGRLLPA"
     gene            1193..2782
                     /locus_tag="VMZ33_06165"
     CDS             1193..2782
                     /locus_tag="VMZ33_06165"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HUR16849.1"
                     /translation="MTGQCDVLISGGMIVDGTGAPGRPGSVAITGDTLRVLDGDAAEP
                     SAGRMIDATGKVVSPGFIDLHSHAGLMVLADPRHEPKVRQGVTTEIIGIDGLSYVPLP
                     SQSDLDSLVEMNSGLDGAPDIAYDWDSVSAYLDRFDGKVAVNIGLLIGNTALRISRLG
                     WSDVPADGSAVADMRAMLRESMAAGALGLSSGLDYPPGAYATTDELAALTNEAAKHGG
                     FYHTHVRYSLGDRFLDPFREAIEIGRRGGGPSHITHFYHRETHPGRPELLLGLVDDAR
                     AEGLDITFDCYPYEWASTRMLIQMPLWIQDGGPGPLKERLADRSARERLRTELRQRGA
                     VYTSPAGWADVRLGYFGQPENVHWEGRSLADVMSELGVDAVDAMCDLLLSESLHINQV
                     TSGPWAKTLAPFVVHPHGMFGTDSTFIGEKPSPRSYGSFPRVLGEFVRDRALLGLEEA
                     VRKMTTAPAARLGLRDRGRLTDGYKADVVVFDPANVRSNATYEDPRRFPSGIDYVLVN
                     GQVVVHAGEHTGALPGRVVSR"
     gene            complement(2793..3293)
                     /locus_tag="VMZ33_06170"
     CDS             complement(2793..3293)
                     /locus_tag="VMZ33_06170"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HUR16850.1"
                     /translation="MLTADPVLALLLFLHVMGAIIAFGPTYAFMILGPMSGREPQHAN
                     FAVRFQHLISTRVILPLALLQGITGLLLVWRTGINLFGTMWLLVAIALYVVALLIGFL
                     VLLPTAGRLVEATSGPPPPRDPDAPPPSGPPPHIAALVRRARMAGMTNGVLIVVIVFL
                     MVTKPF"
     gene            3347..>3780
                     /locus_tag="VMZ33_06175"
     CDS             3347..>3780
                     /locus_tag="VMZ33_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16851.1"
                     /translation="MTITTRPPRVAPRPEAPLAPVHAPESGDLGVGRARAFPLRREGP
                     SKLNGTALYTDDMVFPGAWYGHTIRSTEAHARLLGIDFDPSFDWSRVVVVTAADIPGP
                     NVVSLIQDDQPILVSDEIRHQAEPIALLAAPDRETLREARKR"
BASE COUNT          621 a         1255 c         1287 g          617 t
ORIGIN      
        1 ggcggagacg ccggcgtgga cgcgttcgac ctccgtccga tggtgcttat gccgggcctc
       61 atcgacctgc atgcgcacct gccccaatac ccgaacgctg ggctcggcgg cggcctcgac
      121 ctgctgactt ggctcgagcg ctacatcttc ccgctggagc gtggcttcga ccgcgcggcg
      181 gccgagcgac tcgcgcccgt cgtctttcgc gcatttgcag cggccggcac aacgacggcg
      241 gtgctgtatg gcgcggtgta tgagcacagc ctgcacgctt gtttcgaggc ggcagaggca
      301 cacgggatcc gcgcggtaat cggcaaagtg atgatggatc gactgcgcta cgacgggaag
      361 gtcggcgacc gcgaggtgct ggacgtttcg ctgcgacagt cgcagaacct catcgaccgc
      421 tggcatggcg cggccgacgg ccggctccag tacgtcgtca caccgcgatt cgccgtgagc
      481 tgctcccggg agatgttgcg cgagtccgcg gacctggcga agtcgagcgg cgcctattgg
      541 caaacgcacc tgtccgagga taaccacgag ctcgacgagg ttcgcgagct attcgcggaa
      601 gcggccgatt acaccgatgt gtacgaccgc gccggcggcc tcggaccgag gtcgatcttc
      661 gggcacgcca tccacctttc cgagcgcgag atcaaccggc tggctgagac gagctcgaag
      721 atcgcccatt gcccgagctc gaacctgttc ctggcaagtg gcgcgatgcc gctgtcgcgc
      781 tacatcgagg ccggactggt cgtgggtctg gggagcgacg tcgccggcgg gccggacatc
      841 agcctctttt cggtgatgcg cgccggcgcg tacacgcaga gcggcctgcg cacgatgctc
      901 ggcgagacgc ggccgcagct gacccccgtt gagtggctcc gtatggccag cctggacggc
      961 gcgcgcgcac tcggcctgga ggacacgatc ggctcgctcg aggtcggcaa ggaggccgat
     1021 ttcatctgcg tcgacccctc ggtgacggcg cccgtcgccg gcgacggcga tcgcgacgac
     1081 ccggccgagc tcatgagcca gctgatctac cggtcgcgtc cggacatggt caagggcgcg
     1141 tgggttcgag ggcgattgct acccgcatag tggcaatcgg tacgcttcgc cagtgactgg
     1201 acagtgcgac gtgctgatca gcggcggaat gatcgtcgat ggaacgggcg cgcccggccg
     1261 accggggagt gtcgcgatca ccggtgacac cctgcgcgtc ctcgacggcg atgcggccga
     1321 gccgagcgcg ggacgcatga ttgacgccac gggcaaggtc gtcagcccgg gcttcattga
     1381 cctccattcg cacgccggcc tgatggtcct ggccgacccg cgtcacgagc ccaaggtgcg
     1441 ccagggcgtg acgacagaga tcatcggcat cgacgggctg tcgtacgtgc ctcttcccag
     1501 ccagtccgat ctggactctt tggtggagat gaactcgggg ctcgacggcg ctccggatat
     1561 tgcctacgac tgggactcgg tcagtgccta cctggatcgc ttcgacggca aggtggccgt
     1621 gaacatcgga ctgctcatcg gcaacaccgc gttgcggatc agtcggctgg gttggtcgga
     1681 cgtgcctgcc gatggatcgg ctgtcgccga catgcgcgcc atgctccgcg agtcgatggc
     1741 agcaggcgcc ctgggcctgt catcgggcct cgactacccg cccggcgcct acgctacgac
     1801 cgacgagctt gccgcgctca cgaacgaggc cgccaagcac ggcggcttct accacacgca
     1861 cgtccgctat tccctgggag accgctttct cgatccattc cgcgaggcga tcgagatcgg
     1921 ccgacgcggc ggtggaccgt cccacatcac gcacttctac caccgcgaga cgcacccggg
     1981 ccgacccgag ctgctgctcg ggctggttga cgacgcgcgg gccgaagggc tcgacatcac
     2041 attcgactgc tacccgtacg agtgggcctc gactcgaatg ctcatccaga tgccgctctg
     2101 gatccaggac ggcggcccag ggcccctgaa ggagcgtctg gccgaccgat ctgcgaggga
     2161 gaggctgcgc accgagctcc gccagcgtgg cgccgtctac acgtcacctg ccggctgggc
     2221 cgacgtccgc ctgggctatt tcggtcagcc ggagaacgtg cattgggagg gccgctcatt
     2281 ggccgacgtg atgtcggagc tgggcgtcga cgcggtcgat gcgatgtgcg acctgctgtt
     2341 gtcggagagc ttgcacatca accaggtgac ctctggcccg tgggcgaaga cgttggcccc
     2401 gttcgtcgtt catccccacg ggatgttcgg gaccgattcg acgttcatcg gcgagaagcc
     2461 atcaccgcgc tcatacggct cgttcccgcg ggtgctcggc gagttcgtcc gcgaccgtgc
     2521 gttgctgggt ttggaggagg ccgttcgcaa aatgacgacc gcccccgcag ctcggctcgg
     2581 gcttcgggat cgtggccggt tgactgacgg ctataaggcc gacgtcgtgg tcttcgaccc
     2641 cgcgaacgtg cgcagcaatg ccacgtacga ggacccgcgc cgcttcccgt cggggatcga
     2701 ctacgtcctc gtcaacggcc aggttgtcgt tcatgctggt gagcacacgg gcgcgctgcc
     2761 agggcgagtc gtcagccgat agacgggcga ggctagaaag gcttcgtcac catcaggaac
     2821 acgatgacca cgatgagcac gccgttggtc attccggcca ttcgcgcccg gcgcacgagc
     2881 gccgcgatgt gtggcggcgg gccggagggc ggcggagcat cggggtcacg tggtggtggc
     2941 ggccccgacg tcgcctcgac gaggcggccg gcggtcggaa ggagcaccag gaagccgatg
     3001 agaagcgcca cgacgtagag cgcgatcgct accagcagcc acattgtgcc gaacaggttg
     3061 atcccggtcc gccagacgag caacagcccg gtgatgccct ggagcagggc cagtggaagg
     3121 atgactcgtg tagagatgag gtgctggaac cgcaccgcga agttggcgtg ttgtggctcg
     3181 cggccgctca tgggccctag gatcatgaac gcataggtag gaccgaacgc gatgatcgcc
     3241 cccatcacgt gcaggaacaa gagcaaggcc agaaccggat ccgctgtgag caaccctctt
     3301 ccctcctgcc ccctctgcag ccgcggcaaa cgtatcatcg gtgcgaatga cgattacgac
     3361 gcgtccgccg cgtgtcgctc cacgccctga ggcgccactg gcgcccgtcc acgcgccgga
     3421 atcgggcgac ctgggagtcg gccgcgcccg ggcctttccg ctgcgccgcg agggcccgtc
     3481 caagctcaac ggaacggctc tctacaccga cgacatggtc tttccgggcg cctggtacgg
     3541 gcacacgatc cggtcaacgg aggcgcacgc gcgcctgctg ggcatcgact tcgatcccag
     3601 cttcgattgg tcgcgtgttg tcgtggtcac cgcggccgac atcccggggc cgaacgtcgt
     3661 cagcctgatc caggacgacc agccgatcct cgtttccgac gagatccgac accaggccga
     3721 gccaatcgcg ctgctcgccg ccccggaccg cgagacgctg cgggaggcac gcaagcgcat
//
LOCUS       DATLCO010000263         3006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5690109, whole genome shotgun sequence.
ACCESSION   DATLCO010000263 DATLCO010000000
VERSION     DATLCO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5690109"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..605)
                     /locus_tag="VMZ33_06180"
     CDS             complement(426..605)
                     /locus_tag="VMZ33_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16852.1"
                     /translation="MTARFDGLWLGIVVIAGASFLAIVLGLLLVDFPSRVVIGLLWLG
                     IASWFAWGMWRSRIR"
     gene            complement(602..2698)
                     /locus_tag="VMZ33_06185"
     CDS             complement(602..2698)
                     /locus_tag="VMZ33_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUR16853.1"
                     /translation="MIERVPLYPLAPVLFVSAVVLFVLLMSRHLRVFAYARGSAQVGD
                     EPGRIYSLMKYAIAQVRMFRDIDAGLMHAAIFWGFVVLTVGTFDRVAFGIVESVVRPL
                     FDGWLWRLLLLGQNAFVLTVLAAVGYALFRRLVWRHPRRVTLSRDAIVILLLIGGVVV
                     TEWFAEAFRISAYDDPDKGWAIAAYPLSLVLDALVPDNFMQAGYGLFFWANVAIVSLF
                     LVYLPRSKHLHIVTAFFNAAMRKLRPRGELPKMDLETTDPNARFGIKTVDDMSWKDLL
                     DGFTCTECGRCQEACPAWATGKPLNPKTLIMGVREMAVESEAGVPLIPWIKASTPPGG
                     QATPVVDTAIPFDAVWDCLTCGACVEACPVMIEHVDKIVGLRRNLVLEESRFPAELTQ
                     SFTNMERYQNIWGQPQASRLDWAKGLPFEVPTAASVVSAGGQDAVSELECLYWVGCAA
                     SFDDRNRRVARAVVTCLEAAGVRYAVLGQEESCTGDPARRMGNEYVFQMLAGGNVETL
                     NRYKPKTIITACPHCFNTIGNEYSQLGGTYNVVHHSQYLSRLVGEGRLRVDAVSAGSV
                     TVHDSCYLARYNGVISETREVLGAVPGLELREMEKNGKQTFCCGAGGGRMWMEEKRGT
                     RINAERTRQALETEAETVATACPFCLVMMRDGVADSGERGEGVRVQDISEILAAGLAA
                     SGSGVPKNGRSLPVVQ"
     gene            complement(2695..>3006)
                     /locus_tag="VMZ33_06190"
     CDS             complement(2695..>3006)
                     /locus_tag="VMZ33_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HUR16854.1"
                     /translation="VICTGGTGLGPRDRTPQALNDLLDYEIPGFGEQMRAFGRTQTPM
                     ADLSRSLAGVLGRTLVVAAPGSPRGALDSLTAIEPILEHALETLGGRTQHVETPEGHT
                     P"
BASE COUNT          551 a         1019 c          966 g          470 t
ORIGIN      
        1 cgaccatgtc ccaggggaac tcggcctgtt ggtcgatctc ggcggcgcgg gcggcgacgg
       61 gcccgtctgc aagctccgcg acggcgtcgc gcagcgcgag atgctcttcg gtgaactggg
      121 tcatcgcctc gatgctagcg caggcgcgcc gccgggccgc tcgggctcac cgtatgcacc
      181 acatgattgc ccatgcgtga gacgaagcgt tacagcgccc gcgtcgcccc caggcgacgt
      241 ccgccgtagc gttacccccc ggatggcctg gtttgagtca tgcgcatgct acggaggggc
      301 cgaccaagca catggaacgc gacgaagggc gtctcgagga ctcacccgcc tgcttatcga
      361 ttggtgacct tctccggcag ttatgtgacg catgggttgc aggcgaggcc gatcaggccg
      421 aaccgtcagc ggatgcggga gcgccacatc ccccaggcga accacgacgc gatccccagc
      481 cacagcaggc cgatgaccac gcgggatggg aagtcgacca acaacagccc gagcacgatc
      541 gccaggaaac tggcgccggc gatgaccacg atccccagcc acaggccgtc gaaccgggcg
      601 gtcactgaac gacgggcaga ctccgcccgt tcttcggaac gcccgacccc gaagctgcca
      661 atccggctgc caggatctcc gagatgtcct gcacccggac tccctccccg cgctcgcccg
      721 agtcagccac gccgtcacgc atcatcacca ggcagaaggg gcaggccgtg gcgacggtct
      781 cggcctccgt ctcgagtgcc tgtcgagtcc gttcggcgtt gatccgtgtc ccgcgcttct
      841 cctccatcca catccggccg ccgcccgcgc cgcagcagaa cgtctgcttg ccgtttttct
      901 ccatctcacg cagctcaaga ccgggcacgg cgccgagcac ctcgcgcgtc tcggagatca
      961 cgccgttgta tcgggccagg tagcacgagt cgtggacggt gaccgagcca gcgctgacgg
     1021 catccacccg gagccgcccc tcccccacga gccgcgacag gtattgcgag tgatggacga
     1081 cgttgtatgt gccgcctagc tgtgagtact cgttgccgat cgtgttgaag cagtgcgggc
     1141 aggccgtgat gatggtcttt ggcttgtatc ggttgagcgt ctcgacgttc ccgcccgcca
     1201 gcatctgaaa gacgtactcg ttacccatgc gccgcgcggg atcacctgtg cacgactcct
     1261 cctggccgag cacggcgtat cgaacacctg ccgcttccag gcacgtgacg actgcccggg
     1321 caacgcgtct gttgcgatcg tcgaacgagg cagcacagcc gacccagtac aggcattcca
     1381 gctctgagac ggcatcctgg ccaccggctg acacgaccga cgcggcggtc gggacctcga
     1441 aaggcaggcc cttcgcccag tcgaggcgag aggcctgcgg ctggccccag atgttctggt
     1501 aacgctccat gttggtgaag ctctgtgtga gctccgccgg aaagcgcgac tcctcgagca
     1561 ccaggttgcg gcgcaggccg acgatcttgt cgacgtgttc gatcatgacc gggcacgctt
     1621 cgacacaagc gccacacgtc aggcaatccc acacggcgtc gaacgggatg gccgtgtcaa
     1681 ccaccggggt ggcctgaccg ccgggcggcg tgcttgcctt tatccatggg atgagcggga
     1741 cgcctgcctc cgactcgacg gccatctcgc gcacacccat gatcagcgtc ttggggttga
     1801 gcggcttgcc cgtggcccaa gccgggcatg cctcctggca acggccgcat tcggtgcagg
     1861 taaacccgtc gaggaggtct ttccatgaca tgtcgtcgac cgtcttgatg ccgaaacgcg
     1921 cattgggatc cgtggtctcg aggtccatct tgggcagctc gccgcgtggc ctgagcttgc
     1981 gcatcgcggc gttgaagaac gcggtgacga tatggagatg cttcgagcgc ggcagataca
     2041 ccaggaagag gctcacgatg gcgacgttcg cccagaagaa caggccatag cccgcctgca
     2101 tgaagttgtc gggcacgagg gcgtcgagca ccagtgacag cgggtacgcc gcgatcgccc
     2161 agcccttgtc ggggtcgtca tatgcagaga tacggaatgc ctcggcgaac cattcggtca
     2221 cgacgacgcc gccgatcagc agcaggatga cgatcgcgtc gcgcgacagc gtcacgcgcc
     2281 gggggtgccg ccagaccaga cgtctgaaga gcgcgtagcc gaccgccgcc aggaccgtca
     2341 gcacgaacgc gttctggccc agcagcagca ggcgccacaa ccaaccatcg aagagcggtc
     2401 ggacgacgct ttcgacgata ccgaaggcga ctcggtcgaa tgtcccgact gtcagcacca
     2461 cgaagcccca gaagatggcc gcgtgcatca gaccggcgtc gatgtcgcgg aacattcgga
     2521 cctgcgcgat cgcgtacttc atgagcgagt agatgcgccc aggctcgtca ccgacctgcg
     2581 cgctgccgcg cgcgtaggcg aacacgcgca ggtgacgcga catcaggagc acgaacagga
     2641 caacggcact cacaaaaagc accggcgcca gcggatagag cggcactcgc tcgatcacgg
     2701 cgtgtgaccc tccggcgtct ccacatgctg cgttcggcca ccgagcgtct cgagggcgtg
     2761 ttccaggatc ggttctatgg cggtaagcga atccagggcg ccgcgcgggc tgcccggcgc
     2821 cgcgacgacc agggtgcggc cgagaacgcc agcaaggctg cgtgacagat cggccatcgg
     2881 cgtctgcgtc cgaccaaagg cgcgcatctg ctcaccaaag cccggaattt cgtagtcgag
     2941 aaggtcattc agcgcctgcg gcgtgcggtc gcgcggcccc aagccggtgc cgccggtgca
     3001 gatcac
//
LOCUS       DATLCO010000264         2798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2713997, whole genome shotgun sequence.
ACCESSION   DATLCO010000264 DATLCO010000000
VERSION     DATLCO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713997"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VMZ33_06195"
     CDS             complement(<1..632)
                     /locus_tag="VMZ33_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUR16855.1"
                     /translation="MRIAVLSDIHGNLPALDAVLAKLTPYDAIWQLGDIVGYGPLPNE
                     VVARLAADKATGVRGNHDSAAIGQLETDSFNDDARAAVEWTAETISAETKQWLEALPL
                     QMVDSSFTLVHGSPRDPTWEYIFTAETARANMKKFQTSYCLVGHTHVPFVFVQGKGGR
                     VDAAVATDDSVLDLDGTRIIVNPGSVGQPRDGDPRASAMMIDTEASTIQW"
     gene            complement(666..1547)
                     /gene="rsmG"
                     /locus_tag="VMZ33_06200"
     CDS             complement(666..1547)
                     /gene="rsmG"
                     /locus_tag="VMZ33_06200"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HUR16856.1"
                     /translation="MGRGQRNGSESFGHGRHSDRSSLLRRNTLPDSPAELPELPAEFW
                     RTLEDGLLLLDLTLSPPARAAIDAHVRLLVAWNEQINLTGLRTPQQIARHNVFDALLA
                     LPLMSGWKGAKSLIDIGSGGGFPGLPLALTLPLERVALVDSIGKKQRFLSVASVAATD
                     ALRTAGAEPPQIAALAARAESLAADPEQRETWDVMTSRAVGSVAEVAELGLPLIRRGG
                     RVVAWKRDAGDGSLQREVADARRIVQACGGVPPRIVALPRAAAVGLDGHCLVVIAKAR
                     PTPERYPRDPAERRRSS"
     gene            complement(1871..2230)
                     /locus_tag="VMZ33_06205"
     CDS             complement(1871..2230)
                     /locus_tag="VMZ33_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HUR16857.1"
                     /translation="MTTPKTSAPTTTPGHGEGPTRHSYTKDRAQLLRRLSRMEGQVRG
                     ISRMIEREEYCIDILQQTAALRAAVDAVSLLLMEDHISGCLVTAVKTGEAQTYTDEVI
                     EVVRRSMGRPVRTRAAD"
     gene            2351..2710
                     /locus_tag="VMZ33_06210"
     CDS             2351..2710
                     /locus_tag="VMZ33_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16858.1"
                     /translation="MDGPDWLQTQLTSFTCPACGRRYRGSKIRLLAERDGLFFVDLDC
                     ARCGSHTVAIVTVEIDDTSASITDVSDVSLAVDALADFIDEGMPLGGEPVSADDVLAM
                     HEFLESFDGDMKTLLAA"
BASE COUNT          444 a          901 c          949 g          504 t
ORIGIN      
        1 tgccattgga tggtgctggc ttcggtatcg atcatcatcg cgctcgcccg cgggtcgccg
       61 tcacggggct ggcccacgct gccagggttg acgatgatgc gcgtgccgtc gaggtcgagc
      121 acggagtcat ccgtggcaac cgccgcgtcg acgcggccgc ccttgccctg cacgaacacg
      181 aacgggacgt gcgtgtggcc gaccaggcag tagctggttt ggaacttctt catgttcgcg
      241 cgcgctgtct cggcggtgaa gatgtactcc cacgtcgggt cacgcgggct gccgtgaacg
      301 agcgtgaacg agctgtccac catctgaagg gggagagcct cgagccactg tttggtctcg
      361 gcgctgattg tctctgcggt ccactcgact gcggcgcggg cgtcgtcgtt gaacgagtcc
      421 gtctccaact gtccgattgc ggctgaatcg tgatttccgc ggacgccggt ggccttatcg
      481 gcagcgagcc gagctacgac ctcattgggg agtggtccgt agccgacgat gtcgcccagc
      541 tgccagatgg cgtcgtaggg ggtcagcttt gcgagcacgg cgtcaagcgc cggcagattg
      601 ccgtgaatgt ccgagagcac ggcgatccgc attgaggagt gacgatagca aaggcagcgg
      661 agaagtcacg atgaccggcg tcgctccgcg ggatcccgtg gatatcgctc cggcgtcgga
      721 cgtgccttag cgatcacgac gaggcagtgg ccgtccagcc cgacggcagc cgctcggggg
      781 agtgccacga tgcgtggcgg cacaccgcca cacgcctgga cgatgcgccg cgcgtcggca
      841 acctcgcgct ggaggctgcc atcgccggcg tcgcgcttcc acgccacaac gcgaccgccg
      901 cgccgaatca gtggcagccc cagctcagcc acctcggcta ccgacccgac tgcgcgggag
      961 gtcatgacat cccacgtctc ccgctgctca gggtcggcgg cgaggctctc ggcacgggcc
     1021 gccagcgcgg ctatctgcgg cggttcggcg ccagccgttc gcaacgcatc ggtggccgcc
     1081 accgaggcga cggacagaaa gcgctgcttc ttgccgatcg agtcgaccag cgcaactcgc
     1141 tccagcggca gggtcagggc caggggcagc ccggggaagc cgccgccgct gccgatgtcg
     1201 atgaggctct tcgcaccctt ccagccgctc atcagcggga gggcgagcag ggcgtcgaac
     1261 acgttgtggc gcgcgatctg ttggggcgtc cgaagcccgg tcaggttgat ctgctcattc
     1321 caggcgacga gcagccgcac atgggcgtcg atcgcagcgc gtgccggcgg ggaaagcgtc
     1381 agatcgagca gcagaaggcc atcctcgagc gtgcgccaga actcggcagg cagttctgga
     1441 agctcggcgg gggagtcggg gagagtgtta cgccgcagga gagaggaccg atcagagtga
     1501 cgtccatgcc cgaacgactc tgagccgttg cgctgtcctc gccccatgcg gcgtgacgct
     1561 tcttgcacca ccggcctttc agttttccct cgtcgcccgg tttgtccgat cggatttctc
     1621 cgatcgccgg aagtcggcga ggttaccgta cttggactgg ggcgccgtcc ccgctagggc
     1681 gcgctccagt ggtgcaggcc gcgcatgctc gcgatcgggc ggccagtggg tcaaccggtt
     1741 tttccacgac ttccccagag ctctggcgtt ctgtggagaa gtgctcgaaa tctgccgcct
     1801 gaatgtaaat cctgtggatg gccgggttga cagacgagtg aggcagcggg gttttgcgct
     1861 gccaaattct ttagtcggct gccctcgtcc gtaccggtcg gcccatgctg cgtcggacga
     1921 cttcgatcac ctcgtcggta taggtctgtg cctcgcccgt cttcaccgct gtcaccaggc
     1981 agcccgagat gtggtcctcc atcaacagca gtgaaaccgc gtcgaccgcg gctcgaagcg
     2041 cggccgtctg ctggaggatg tcgatgcagt actcctcgcg ctcgatcatg cgtgaaatgc
     2101 cacgcacctg gccctccatg cgcgacaggc gacgcagcag ctgcgcgcgg tccttggtgt
     2161 agctgtgccg ggtcggccct tcgccgtggc cgggcgtcgt cgtcggggcg ctcgtcttgg
     2221 gtgtcgtcat actggtgggg agtatacggg agccagtcgt ccgaacggcg cgcacattcg
     2281 aaagggacgc gcaacctctt gcacggcgct gggagcaggg caccgacgat acgggctaga
     2341 ctcgctctcg atggacggtc cggactggct gcaaacccag ctgacatcgt tcacctgtcc
     2401 tgcctgcggt cgacgctacc gcggcagcaa gatccgcctc ttggccgagc gtgacggact
     2461 cttcttcgtg gaccttgact gcgcccgctg cggcagtcac acggttgcca tcgtcaccgt
     2521 cgagattgac gacacaagcg cctccatcac ggatgtctcc gacgtgagtc tcgccgtcga
     2581 tgcgctggcg gacttcatcg acgaaggcat gccgctcggc ggcgagcccg tctccgctga
     2641 cgacgtgttg gccatgcacg aattcctgga gtcgttcgac ggcgacatga agacattgct
     2701 cgccgcgtga gggaggcggc ggctcggttc gcgcacgcca gtgaagctga gctggctcgg
     2761 atcctcgact actacggagt ccggtggcag tacgagcc
//
LOCUS       DATLCO010000265         2011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2717815, whole genome shotgun sequence.
ACCESSION   DATLCO010000265 DATLCO010000000
VERSION     DATLCO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2717815"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1072
                     /locus_tag="VMZ33_06215"
     CDS             320..1072
                     /locus_tag="VMZ33_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR16859.1"
                     /translation="MNDQRIGAAFRAVRVRRGWRQSDVATRAGVSAGVVSLIERGHLP
                     HVSTLAFRAVASELEIRADIVLSLPHGELDRLLNAGHAELHEGIARYLNDLPGWLHAP
                     EVSFAFYSERGVIDILAFHPPSGSLLIIELKTELVSLENLLTTMDVRVRHAAAIAQAR
                     GWVTRSVSAWIVFADTATNRRRVAAHRSTLRSAFPADGRAMRSWLREPDRPLRALSFW
                     SNSNVTTPSRLARGCRRVRVRKTAASPALTAA"
     gene            complement(1160..>2011)
                     /locus_tag="VMZ33_06220"
     CDS             complement(1160..>2011)
                     /locus_tag="VMZ33_06220"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HUR16860.1"
                     /translation="MEITHVIRGEDHLSNTPKHILLFRAMGAAIPEFAHLPLILNPDR
                     TKMSKRKSQTAVADYRAQGYIPEAFVNFLALLGWSSGSEEEIFSLSELGERFDLERVN
                     SGGAVFDRERLEWMNGQWIRKLSDEELVERLLPFLAGGPGRAPSAEEVRPLIPMIRER
                     LPTLSAVGPLIDFLFIEELVVEPDAIVPKRWDPGLTLEALTAARHVIADVGTVSFEAD
                     ELEPPLRALCEERGWKAGDLFMAIRVAVTGRTATPPLFDTLVALGYERTLARLDAARE
                     SLESLGS"
BASE COUNT          345 a          662 c          640 g          364 t
ORIGIN      
        1 cttggcctcg agccagttct cgacgatcgc gtcgcggccc ttcaggggtt cgtcctgggg
       61 gtggtaccga tactcggcat cctcgctcca caggccggca atcgcgtcag gctcgtacgt
      121 cttccacgcc tcgacgtagc ggtcgagcca tttctggaaa cccagtcgat ccacgtcctt
      181 gctcctcgtc ccgcttgttc gccagtaacc gtccaaggca ccttataaag ttactcaata
      241 ctgcgtcaag cgacagcagc gtcgattgtg gacggattgg tttgtctgca gtgaagtcct
      301 cctagccatc attgccaacg tgaatgacca acgcatcggc gcagccttca gggcagtgcg
      361 cgtccgtcgc ggctggcgac agagtgacgt cgcgacccgc gccggcgtat cggccggggt
      421 cgtctcgctt atcgagcgcg ggcatctccc acatgtttca acacttgcct tcagggcggt
      481 cgcgtcggag ttggaaattc gggccgatat cgtgctcagc cttccgcacg gtgaacttga
      541 ccggctgctc aatgcgggtc acgcggagct gcacgaagga attgctcgct acctgaacga
      601 cttgcccggc tggctccacg ccccagaggt gtcattcgcg ttctactccg aacgaggcgt
      661 gatcgacatc ctggcctttc atccgcccag tgggtcactg ctcatcatcg agctgaagac
      721 ggagcttgta tctctggaga acctacttac gacgatggac gtgagagtcc ggcatgctgc
      781 ggcgatcgca caggcgcgcg gctgggtaac gcgttcggtc agcgcctgga tcgtgttcgc
      841 tgacacggcg actaaccgac gtcgcgtcgc cgcacaccgc tcgacgctgc ggtcggcctt
      901 cccggcggat gggcgcgcga tgcgcagctg gctccgtgaa cccgatcgac ctctcagggc
      961 gctgtctttc tggtctaact cgaatgtgac aacacctagc cgactcgccc gagggtgtcg
     1021 gcgcgtccgc gtgcggaaga cggcggcgtc gcctgcgctt acggcagcgt gacgtaaaaa
     1081 ggcgaagagg gacgccgttc gggccggcta gtgatcgtca ccttcaaact ggcgcaagaa
     1141 tgggacctca ggggtcgggc tagctgccta gggactcgag cgactcgcgg gcggcgtcca
     1201 ggcgggcgag cgtccgttcg tagccgagcg ccacgagcgt gtcgaacaat ggtggcgtgg
     1261 cggtccggcc ggtgacggcc acgcggatgg ccatgaacag gtcgccggcc ttccagccgc
     1321 gttcctcgca cagggcacgc agcggtggct cgagctcgtc cgcctcgaac gacaccgtgc
     1381 ccacgtccgc aatgacgtga cgcgccgccg tcagcgcctc cagcgtcaac cctgggtccc
     1441 agcgcttggg cacgatcgcg tccggctcga ccaccagctc ctcaatgaac aggaagtcga
     1501 tcagcggacc aaccgccgac agtgttggca ggcgctcgcg gatcatgggg atgaggggcc
     1561 gcacctcttc agcgctcggc gcacgccccg gcccgccagc caagaacggc agcagtctct
     1621 caacgagctc ctcgtcagac agcttgcgga tccactggcc gttcatccac tccagccgct
     1681 cacggtcgaa cacggcccca cccgagttga cccgctcgag gtcaaaccgc tcgcccagct
     1741 ccgacagcga gaagatctcc tcctcggacc cgctcgacca gccgagcaag gccaggaagt
     1801 tgacgaacgc ctccgggatg tagccctggg cgcggtagtc ggcgacggcg gtctgcgact
     1861 tgcgcttgct catcttggtg cggtcgggat tgaggatcag cggcaggtgc gcgaactcag
     1921 ggatcgcagc gcccatggcc cggaaaagca ggatgtgctt gggcgtgttc gagaggtggt
     1981 cctcgccgcg gatcacgtgg gtgatctcca t
//
LOCUS       DATLCO010000266         3690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1234006, whole genome shotgun sequence.
ACCESSION   DATLCO010000266 DATLCO010000000
VERSION     DATLCO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234006"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VMZ33_06225"
     CDS             <1..570
                     /locus_tag="VMZ33_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16861.1"
                     /translation="AELEQLVSDITPAWHATALAQTLLKLTAPGVPDTYQGTEIWDLS
                     LVDPDNRRAIDWELRRELLDRAVRSTAAEAMADSNSGLPKIWLIKRVLDLRSQQPELF
                     GTVARYQPIAASGVESDRVIAFVRGGEAVTVAPRFNLRGGEWRDTAIELAAGEWRNVL
                     TDETVAGGRVEIGRLLSGFPVAVLVPQVG"
     gene            570..2309
                     /gene="treZ"
                     /locus_tag="VMZ33_06230"
     CDS             570..2309
                     /gene="treZ"
                     /locus_tag="VMZ33_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HUR16862.1"
                     /translation="MGRRLSVWAPYATRVEAVLDAGRRVAMSGAERHGWWSTADEFPA
                     GTDYQFSVDGSDPRPDPRSMHQPEGVNGPSRTFDPSVLPWTHRPWEPPALADALVYEA
                     HIGTFSDAGTFDGAIGHLDHLVDLGVTHLEVLPINEFPGKRGWGYDGVDLYAAHSAYG
                     GPQGFARLVDACHGRGLAVILDVVYNHLGPSGNYLGEFGPYFTDKYRTPWGMAVNFDD
                     ADSDEVRRFFIDNALTWLRDFHVDALRLDAVHAIVDTSALHVLEQLQREVRELSAETG
                     RRYAVIAESDLNDPRLITDTDQGGYGLDAQWSDDFHHALHSVLTGETSGYYADFGTLG
                     DLATTLTRGYRYNGDYSPFRKRRHGRPLGEMSGGGLLGYLQNHDQIGNRATGERSSQL
                     MSEGRLMIGATLVVTAPFVPMLFQGEEWAASTPFQYFTDHEDRDLGRAVTEGRTHEFA
                     AFGWKPMDVPDPQDPQTFERSKLRWEELLEPGHARVLDWHRRLIALRRERPELRDGRL
                     GAVAVQHDAAVGWLWFRRGRLVVAFNIGGDVARLELDGAHAVVLASSEGVNVDGSSLS
                     LPPDSVAILESAV"
     gene            complement(2306..3427)
                     /locus_tag="VMZ33_06235"
     CDS             complement(2306..3427)
                     /locus_tag="VMZ33_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUR16863.1"
                     /translation="MRGEAWLWFVRGGGFLMGAAIAFGLIYTMIVGARVVVLVFIALL
                     LASGLEPLIDRIRARTPLGRGATLLLVYFGFFVTVAALLLFVLPSAIDQFGQLGTRLT
                     PLLDDAREWARTLDRGVSLAVVGLIDTIQQAFAPRGVSVPDPEELIALGLTFAEIVIT
                     IASLLTLVYFWLTERARLQRFALALLPYERRAGAREAWNEIEVRLGAWVRGQLILMGS
                     VGVATSIAYFLIGLEGALLLGLIAALCEVIPIIGPALGAVPALIVAALTGRIEIVLIV
                     AVVYFIIQVVEGNILVPLVMHNTIGVPPFLVFVSILGGAAVAGIPGALVSVPLVAALL
                     VLVERLQARDSPVPLSGQAPPEQIPEIAEAVEAMAAKGS"
BASE COUNT          653 a         1208 c         1225 g          604 t
ORIGIN      
        1 gcggaactgg agcagctcgt ctccgacatc acgccggcct ggcatgcgac cgcactcgcc
       61 cagaccctgc tgaagctgac tgcgccgggc gtccccgaca cctaccaggg gaccgagatc
      121 tgggatctca gcctggtcga cccggacaat cgccgagcca ttgattggga gctgcgccgg
      181 gagttgctcg atcgggcagt gcgctccacg gccgcggaag cgatggccga ttccaactcc
      241 ggtctgccaa agatctggct catcaagcgc gtcctggacc ttcggtcgca gcagcccgag
      301 ctcttcggca cggtagcccg ttaccagccg atcgctgcgt caggcgttga gtccgaccgt
      361 gtgatcgcat tcgttcgtgg cggagaggct gtcaccgtgg cgccgcgctt caacctccgc
      421 ggtggcgagt ggcgtgacac cgccatcgag ctggccgccg gggagtggcg gaacgtgctc
      481 accgacgaaa ccgttgctgg agggcgcgtg gagatcgggc gcttgctatc gggctttccg
      541 gtcgcagtcc tggtcccgca ggtcggctga tgggtcgacg gctttcggtc tgggcgccgt
      601 atgcgacgcg ggttgaggcc gtgctcgatg ccggccgccg cgtggccatg tcaggggcgg
      661 agaggcatgg ttggtggtcg accgccgacg agtttccggc ggggacggat tatcagttca
      721 gcgtcgatgg cagcgacccg cggccggatc cgcgctcgat gcatcagccc gagggcgtga
      781 acgggccgtc gcgcacattt gacccgagtg tgctgccctg gacacaccgg ccatgggagc
      841 caccggcact ggcggatgcg ctcgtttacg aggcacatat cggcacgttc agcgacgctg
      901 gcacgttcga cggtgccatc gggcacctcg accaccttgt tgacctcggc gtgacgcacc
      961 ttgaggtcct gccgatcaac gagtttccgg gcaagcgcgg ctggggctac gacggcgtcg
     1021 acctctacgc ggcgcactcg gcatacggcg gcccgcaggg ttttgcccgc ctggtcgacg
     1081 cgtgccacgg ccgtggcctg gcggtcatcc tcgacgtggt ctacaaccac ctcgggccgt
     1141 cgggcaacta cctcggcgag ttcgggccgt acttcactga caagtacagg acgccgtggg
     1201 gaatggctgt caacttcgac gacgccgact cggacgaggt tcgccgcttc ttcattgaca
     1261 acgcgctgac gtggctgcgc gatttccacg tcgatgccct gcgcctcgac gcggtccacg
     1321 cgatcgtcga cacgtcagcc cttcatgtgc tcgagcagct ccagcgcgag gtgcgggaac
     1381 tatcggcgga gaccggacgg aggtacgccg tcatcgccga gtcggacctc aacgacccgc
     1441 ggctgatcac tgacacggat cagggcggat acgggctcga cgcccaatgg agcgacgact
     1501 tccatcacgc tctgcattcg gttctcaccg gtgagacgag cggctattac gccgacttcg
     1561 gcacgctcgg cgacctcgcc acgaccctca cacgtggtta tcggtacaac ggtgactact
     1621 caccctttcg caagcggcgc catggccggc cgctgggcga gatgagcgga ggcgggctcc
     1681 tcggctacct ccagaaccac gaccagatcg gcaaccgcgc gaccggggag cgcagctcgc
     1741 agctgatgag cgagggccgg ctcatgatcg gtgcgacgct cgttgtcacc gcgcccttcg
     1801 tgccgatgct cttccagggt gaggagtggg ccgcgtccac gccgttccag tacttcacgg
     1861 accatgagga ccgcgacctt ggccgtgctg tgactgaggg gcgcacacac gagttcgccg
     1921 cgttcggatg gaaaccgatg gatgtgcccg acccgcagga tccgcagacg ttcgagcgct
     1981 cgaagctgcg ctgggaggag ctcctcgagc ctggccacgc ccgggtcctg gactggcacc
     2041 ggcggctgat cgccctgcgc cgtgaacgcc ctgagctccg cgacggccga ttgggtgcag
     2101 tcgcggtcca gcacgacgct gcagtcggat ggctctggtt ccgccgcggt cggctggtcg
     2161 tcgccttcaa cattggcggc gacgtggcgc ggctggaact ggacggcgcg catgcggtcg
     2221 tgctcgcctc gtcggagggc gtcaatgtcg acggctcgtc gctgtcgctg ccgcccgact
     2281 cggtggccat cctggagtcg gctgtctagc ttcccttcgc cgccatcgcc tcaaccgctt
     2341 cggcgatctc tggtatctgc tcgggtggcg cctgtccgct gagtggcaca ggggagtcgc
     2401 gtgcctggag tcgctcaacg agtaccagca gcgcagccac gagtgggaca gaaacaagtg
     2461 cccccggaat accagcgacc gctgcgccgc cgaggatgct tacgaacacc aggaacggcg
     2521 gcacgccgat ggtgttgtgc ataaccagcg gtacgaggat gttgccctca accacctgga
     2581 tgatgaagta gacaacggcc acgatgagga cgatctcgat cctgccagta agggccgcga
     2641 ctatgagcgc cggaaccgct cccagcgccg gcccgatgat ggggatcacc tcacacagcg
     2701 cagcgatcag gcccaggagc agggcgccct ccaggccgat caggaagtag gcgatcgacg
     2761 tcgcaacgcc gacgctgccc atgaggatca gctggccgcg cacccaagca ccgaggcgaa
     2821 cctcgatctc gttccaggcc tcacgggcgc cggcccgacg ttcgtacggc aagagcgcca
     2881 gagcaaacct ttggagtcgc gcgcgttcgg tgagccagaa gtagaccagc gtcagcaggg
     2941 acgcgatcgt gatgacgatc tcggcgaagg tgaggccgag cgcgatcagc tcctcagggt
     3001 ccggaacgga aactccgcgc ggcgcgaagg cctgctggat cgtatcgatc aagccgacga
     3061 cggccagtga aacgccgcga tcgagcgtgc gcgcccattc ccgcgcgtcg tcgagaagtg
     3121 gcgtcagcct ggtgccgagc tgtccgaact gatcaatggc gctcggcagg acgaagagca
     3181 ggagtgctgc caccgtgacg aagaagccga agtagaccag aagcagggtt gcgcctcggc
     3241 cgagtggagt ccgagctcga atccggtcga tcagcggctc cagccccgac gccagcagga
     3301 gcgcgatgaa gaccaggacg acgacgcggg cgcccacgat catcgtgtag atcaggccga
     3361 aggcgatggc tgcgcccatg aggaagccgc ctccccgaac gaaccagagc caagcctcac
     3421 cgcgcatgtg tagccctcac cgtaaccgag ccggaaccac ccgttccaga gccaaactct
     3481 gcaatatcta ttgcattttg taatgtcctg ggccgacctt gcaggatcag gtggacatcc
     3541 tcccgaatac tctcgacttc cttacggagg tgcagtgaac agtcaaccac tcgtgctcgt
     3601 cgccgatgac gagcccagaa tcaccaaact cgttgccatg acccttcacg acgaagggtt
     3661 tcgtgtcgta accgcggggg gcggcgagga
//
LOCUS       DATLCO010000267         4150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5710364, whole genome shotgun sequence.
ACCESSION   DATLCO010000267 DATLCO010000000
VERSION     DATLCO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5710364"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VMZ33_06240"
     CDS             complement(<1..1112)
                     /locus_tag="VMZ33_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR16864.1"
                     /translation="MVRLGSVAAMTERVRGTAAPTHVAAPADVVASAPVLAAAAPQRL
                     QPVDERAPVIRSKIQPPPVRSSTLSRQRLLDRLTDAVSGRVTLVTAEAGYGKTTLLAD
                     FSARASARCLWYKLDRTDADPITWTNYLIAAAREVDPAFGAATLSLLAQVGAGGPPES
                     AFVASLLGELPRLGEAPTVLVLDDFHLVDGSSEARDYVSRLIKDAPSWLHFVIASRQK
                     PPLELSRIAAGGELAEITTDDLRFTTEETGRLFADGYGLALEPDVLHDLDARTQGWAA
                     SLQLFHGSVRGRPLTAVRALAKSLSGAVNPIYDFLAQEVLNNISDELEEFLVRISLLE
                     RVVPRRVVPLFVDRGSDAPSTAQAEAWIEEADHLGLL"
     gene            complement(1191..1412)
                     /locus_tag="VMZ33_06245"
     CDS             complement(1191..1412)
                     /locus_tag="VMZ33_06245"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HUR16865.1"
                     /translation="MGVVIIRELVGTSPRSWSDAARQAVTTAARTVRNIREIEVVKSS
                     ARVENGEITEYRVELKLHFEYEGSQSGQG"
     gene            1515..2474
                     /locus_tag="VMZ33_06250"
     CDS             1515..2474
                     /locus_tag="VMZ33_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16866.1"
                     /translation="MTARSARHRLLVIPLVALATTIAACNAGAPPSSPPTNPPSSIPT
                     PGPTASPTPTATPEVTPTPNPDLIPHPTGAHDIVLRMEEGGGFVPMGFFFTQSPTFTL
                     YGDGTVIFKQVDNRLNSFNLPALPWLVGHLSEESVQALLQYALADGHLLDAKAQYDNQ
                     MVADAGNTIFTLNAAGQEKVVNIYALFESDMPGVPDAADRAAFWQLRQVLNAFETQEG
                     LGEVEVYDAEWYRLLLSDGFGEPTGEVKDWPWDDLTLADFPAGDEPGGIANLDREHVA
                     LLMDVPNGGHSGVWAIAPDGEEVVNFGVRPLLPEEIEGAGLDD"
     gene            complement(2491..3855)
                     /gene="dnaB"
                     /locus_tag="VMZ33_06255"
     CDS             complement(2491..3855)
                     /gene="dnaB"
                     /locus_tag="VMZ33_06255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HUR16867.1"
                     /translation="MSIDRLPPQSIDAEQSVIGALLIDRDAVIQVADFLKPQDFYRQA
                     HGTVYGAILDLYERREPVDIVTVSETLERGEALEQIGGSAYLTSLINMTPTAVNAIHY
                     GRIVERKAVLRNLIGAAGKIASIGYEDSPEVSEAIDRAEQELFAVSERRVGSGFSPLK
                     TLLHAAYDRLDYLHQHRGEISGTRTGFSDLDTLTSGLQKSDLVIVAARPSVGKTSLAL
                     NFAEHASVTEGKSVGVFSLEMSKEQLVLRLLSSVANIDSQRLRTGFLEEMDFTRLAPA
                     MNALAEAPIYIDDTPNISTMELRTKARRLQAESGLDMIIVDYLQLMQSSTTTRDANRV
                     QEVSEITRGLKGLARELQVPVVALSQLSRQPEMRENKEPRLSDLRESGSIEQDSDLVM
                     FLWREKERGGEEEDPEGEVINLRLAKHRNGPTGDIKLYFRKRQTRFVSIAENSRYANA
                     TYAN"
BASE COUNT          701 a         1370 c         1338 g          741 t
ORIGIN      
        1 aggaggccga ggtggtcggc ctcttcgatc caggcctcgg cttgcgcggt gctcggcgcg
       61 tccgagccgc ggtcgacgaa cagcggcacg acccgccgag gcaccacgcg ttccagcagt
      121 gaaatgcgaa ccaggaactc ctcgagctca tcgctgatgt tgttgaggac ttcctgcgcg
      181 aggaagtcgt agatgggatt cacggcgccc gacaaggact tggccaacgc cctgaccgcc
      241 gtcagtggcc ggccacgcac ggatccatgg aatagctgca ggctcgccgc ccagccctgg
      301 gtgcgggcgt ccaggtcatg caggacgtct ggctccaggg ccagcccgta gccatcggcg
      361 aaaaggcggc ccgtctcctc ggtcgtgaac cgcaggtcgt ccgtggtgat ctctgcgagc
      421 tcgccacccg ccgcgattcg actcagctcg aggggtggct tttgccgcga cgcaatcacg
      481 aagtggagcc atgacggcgc gtccttgatc agccgagaca cgtagtcgcg ggcctcgctg
      541 ctgccgtcga caaggtggaa atcgtccagg acgagaacgg tgggtgcctc acccaaccgc
      601 ggcagctccc cgagcaagct ggcgacgaac gcgctctcag gcggtccgcc ggcacctacc
      661 tgcgccaaca aggacaacgt cgccgctccg aaagccgggt caacctcccg cgccgccgca
      721 atcaggtagt tcgtccaggt gatcgggtct gcgtccgtgc gatcgagctt gtaccaaaga
      781 cagcgagctg aggcccgagc cgagaagtcg gccaagaggg tcgtcttgcc gtagccggct
      841 tccgcagtga ccaacgtgac gcggcctgac accgcatcag tcagtcgatc gagcaagcgc
      901 tggcgcgaga gggtggacga ccgcaccggc ggcggttgga tcttcgaccg gatgaccggc
      961 gcacgctcgt caaccggctg aagccgctgc ggcgcggccg cggccaggac gggcgccgac
     1021 gcaacgacgt cagcaggagc ggcgacatgt gttggcgcgg ccgtaccgcg gaccctctcg
     1081 gtcatcgcgg cgaccgaccc gagtcggacc atggaagcat catcgcgacg ctcccttacc
     1141 gcgctcttac cgcgggatgt ggataactaa ccgcgccgta ccaccctcga ctaaccttga
     1201 ccgctctggc tgccctcgta ctcgaaatgc agcttgagct cgacccggta ctcggtgatc
     1261 tcgccgttct cgacccgcgc gctcgacttc acgacctcga tctcgcggat gttgcgcact
     1321 gttcgcgcag cggtcgtaac cgcctggcga gcagcgtcgc tccaggagcg cggactcgtc
     1381 ccgaccagct cacggatgat gaccacgccc atctgactcc ctctatgacg ctcggttttc
     1441 gagcggcgag tgtacgcggc gcctttgttg ggaaccatct gtgagtcggc cgcgtcaaac
     1501 gccccatgcc gaccatgact gcgcgctccg cgcgccaccg ccttctagta attcccctgg
     1561 tggcgctggc caccacgatc gcggcgtgca acgccggggc gccaccttcg tcgccaccga
     1621 cgaacccgcc ctcttcgatc ccgacgcctg gcccgactgc cagccccacc ccgactgcga
     1681 cccctgaggt cacgcctacc ccgaaccccg atttgattcc gcatccgacc ggcgcgcatg
     1741 acatcgtgct gcgcatggag gagggcggcg gcttcgtgcc gatgggcttc ttcttcaccc
     1801 aatcgccaac attcacgctc tacggcgacg gcacagtcat cttcaagcag gtcgacaacc
     1861 ggcttaactc gttcaacctg ccggcgctac cgtggctggt cggacacctt tcagaggaga
     1921 gcgttcaggc cctgctccag tacgcgcttg cagacggcca ccttctcgac gccaaggcgc
     1981 agtacgacaa ccagatggtc gccgatgccg gcaacacgat cttcacgctc aacgctgccg
     2041 gacaggagaa ggtcgtaaac atctacgcgc tcttcgagag cgacatgccg ggcgttcccg
     2101 atgcagccga ccgcgcggcc ttctggcaac tgcgccaggt cctcaacgcg ttcgagaccc
     2161 aggagggttt gggggaggtc gaggtctacg acgcagagtg gtatcgactg ctcctctcgg
     2221 acgggttcgg tgagcccacg ggcgaggtca aggactggcc gtgggatgac ctgacgctgg
     2281 ccgatttccc cgctggtgac gagccgggcg gcatcgccaa tctcgaccgc gagcacgtcg
     2341 cattgctgat ggacgttccg aacggcggcc actccggcgt ctgggcaatc gcgcccgatg
     2401 gcgaggaggt agtcaacttc ggtgtccggc cgctcctccc cgaggagatc gagggcgccg
     2461 gcctggacga ctgacccctc ggcgtcctgt ctagttggcg tacgtggcgt tggcgtagcg
     2521 gctgttctcg gcaatcgata cgaagcgcgt ctggcgtttg cggaagtaga gcttgatgtc
     2581 gccggtgggg ccgttgcggt gcttggccag ccggaggttg atcacctcgc cctccggatc
     2641 ctcttcctcg ccgccacgct ccttttcgcg ccacagaaac atcacgaggt cagagtcctg
     2701 ctcgatggac cccgactcgc ggaggtctga cagccgaggc tccttgttct cacgcatctc
     2761 cggctggcga gagagctgtg acagcgcgac aacaggcacc tgcagctcac gcgccagacc
     2821 cttgagcccg cgcgtgatct cgctcacctc ttgaacgcgg ttcgcgtcgc gcgtggtggt
     2881 gctcgactgc atcagctgga ggtagtcaac gatgatcatg tcgaggcccg actccgcctg
     2941 gagccgacgg gccttggtgc ggagctccat ggtgctgatg ttgggcgtgt cgtcgatgta
     3001 gatcggcgct tccgccagcg cgttcatcgc cggggccagg cgggtgaagt ccatctcttc
     3061 gaggaagccg gtccgaaggc gctgcgaatc aatgttcgcg acgctcgaca gcagacggag
     3121 tacgagctgc tccttgctca tctcgagaga gaagacgccg acgctcttgc cttccgtgac
     3181 cgacgcgtgc tcggcgaagt tgagcgcgag gctcgtctta ccaacggatg gccgcgccgc
     3241 gacgatgacc aggtcgctct tctggagacc gctggtgagc gtgtccagat cggagaagcc
     3301 ggtgcgcgtg ccactgatct cgccgcggtg ttgatgcagg tagtcgaggc ggtcgtacgc
     3361 ggcgtggagc agcgtcttca ggggtgagaa accacttccg acccgccgct cgctgacggc
     3421 gaaaagctcc tgctcggcac ggtcgatggc ctcgctgacc tccggcgagt cctcatagcc
     3481 gatgcttgcg atcttgccgg ccgccccaat caggttccgc agcaccgcct tgcgctcaac
     3541 gatgcgcccg tagtggatgg cgttgacggc cgtgggcgtc atgttgatga ggctggtcag
     3601 gtacgccgag ccgccgatct gctcgagtgc ctcgccacgc tccagcgtct ccgagacagt
     3661 gacgatgtcg accggctctc ggcgctcgta gaggtcaagg atggcgccgt agacggtgcc
     3721 gtgtgcctga cggtagaagt cctgtggctt cagaaagtcc gcgacctgga tgaccgcgtc
     3781 gcggtcgatc agaagggcac cgataaccga ctgttcggcg tcgatcgatt gcggtggtag
     3841 gcggtcgatg gacaagtcca ggtctccgac ggcaacgggt tctaaagtgc ccgatctgct
     3901 cgtccgaccg gcgtcatcac atggtcggac gaccgacccc ggcggacaac cgagttatgc
     3961 agagaatccg tcccccgttt tccaccgggg tgtggacggc tgtggaaagc ccaggtcagt
     4021 cgacccggcg gattaccccg atcagctttc cctggatgtt cacgttgtcg tagaactggg
     4081 gcgggtatga ctcgttcgcc ggctgcaggc ggatgcggtt cttttccttg tagaagcgct
     4141 tgagggtgac
//
LOCUS       DATLCO010000268         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1242882, whole genome shotgun sequence.
ACCESSION   DATLCO010000268 DATLCO010000000
VERSION     DATLCO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242882"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1604)
                     /locus_tag="VMZ33_06260"
     CDS             complement(144..1604)
                     /locus_tag="VMZ33_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HUR16868.1"
                     /translation="MSRAAPVPAVSAAAHERAQPPISTDRTIAQRLAHALGLAQANSL
                     ARRFLLASLLVLGVGGVVIGWWVGNQLERGIIDRSASITGLYVDSIIEPHLATLASGE
                     WLNDRDIAALDELLTNTAFAEDIVALKIWRPDGVIEYSPDRSLVGQAFPVEGDLAAAL
                     KGTVVSEMSSLDDAENVNESALGYDRLLEMYLPIRERGGERIIAVAEFYQVPTELERQ
                     VADARLQSWLVVALAVALAYLLLYGIVRQGSETIDHQRVALETQVSELSALLNQNEQL
                     RQRVRVAAERTTTLSERNLRRISSDLHDGPAQMLALAMLRLDQLRARGASDKEVEELQ
                     STLSDALRDMRAVAAGLRLPELESLSTADVVRRGVDDHERRTSVPVTVTLGELPREAS
                     LPTKIALFRALQELLSNSTRHGGGKDVEVSLVDGDGALRMIVADGGPGFAQTLVGVPG
                     HLGLAGVREQAELLGGTFTIDARAGGGARVTVAWPL"
     gene            1806..2204
                     /locus_tag="VMZ33_06265"
     CDS             1806..2204
                     /locus_tag="VMZ33_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16869.1"
                     /translation="MNSLRKRIVGGTAVGMTALTLLAVPAGVAANDGDIIRTGNCSAG
                     ADWKLKLSPENGRIEVEFEVDQNINGRVWNVSLKRNGNTFFTGQRTTQAPSGSFEVRR
                     VTTNGAGPDTFVARAVDQRTGQVCRGSATF"
BASE COUNT          413 a          835 c          782 g          371 t
ORIGIN      
        1 gttgtgctcg tgcgcgagac ggacggcttc gtcggctgat gcaccctcac ccacgacctc
       61 gacgtccggc tgtgccgaca gcgacgccac gacgcccgcg cggaacatcg ggtgatcgtc
      121 cgcgacgacg atacggatga gctctacagc ggccatgcca ccgtcactcg cgccccgccg
      181 ccggctcgcg cgtcgatagt gaacgtgccg cccagcagct cggcctgctc ccttacaccg
      241 gccaagccca ggtgaccggg aaccccgacc agagtctggg cgaagcccgg cccgccatcc
      301 gcgacgatca ttcgcaaggc gccgtcgcca tcgaccagcg aaacctcaac atccttcccg
      361 ccgccgtggc gggtcgagtt tgaaagcagc tcctgtaggg cccggaagag cgcgatcttc
      421 gtcggcaggc tggcctcacg cggcagctcg ccgagagtca ccgtcacagg caccgacgtc
      481 cgccgctcat gatcgtcgac cccgcgccgt acaacgtcgg ccgtcgacaa cgattccaac
      541 tcgggcaaac gcaggccggc agcaacggcg cgcatgtcac gcagcgcgtc gctcaacgtc
      601 gactggagct cctcgacctc cttatcggac gcaccacgcg cccgcagctg gtcaagccgc
      661 aacatcgcca gtgcaagcat ctgggccggc ccatcgtgca ggtcgctgga gattcggcgc
      721 aggttccgtt cggagagcgt cgttgtccgc tccgcggcca cgcgcacccg ctgccgcagt
      781 tgctcgttct ggttcaagag cgccgatagc tcggacacct gcgtctccag cgcaaccctt
      841 tgatgatcga tggtctcgct gccctggcga acgattccgt acagcagcag ataggcgagg
      901 gccacggcca acgctacgac gagccacgac tgcaggcgcg cgtcggcgac ctggcgctcg
      961 agctcggtcg gcacctggta gaactcggca acggcaatga tccgctcgcc gccgcgctct
     1021 cggatgggca ggtacatctc cagcaaccgg tcgtagccga gggcgctctc gttcacgttc
     1081 tcggcgtcgt cgagcgagct catctcgctg acgaccgtgc ccttgagcgc cgcggcgagg
     1141 tcgccctcca cggggaatgc ctggcccacc agggagcgat cggggctgta ctcgatcaca
     1201 ccgtcgggac gccagatctt gagcgcgacg atgtcctcgg cgaaggccgt gttcgtcagc
     1261 agctcgtcga gagcggcgat gtcccggtcg ttgagccact ccccggaggc gagggtcgcc
     1321 agatgcggct cgatgatgct gtcgacatag agaccggtga tcgatgccga gcggtcaatg
     1381 atcccgcgct cgagctggtt gccgacccac cagccgataa cgacgccacc gacacccagg
     1441 acaagcaggc tcgccagcag gaacctgcgc gcgagcgagt tcgcctgtgc gaggccgagg
     1501 gcgtgagcga ggcgctgagc gatcgtgcgg tcggtgctga tcggcggctg ggcgcgctca
     1561 tgggcagcag cagatacagc gggtacgggc gcggcgcggg acatcgcgga aatcgtacgc
     1621 gcgtgtcgtc gcggctcata tcgaccaacg gaccgaaagg tctcggacca aggtccgttc
     1681 gctttcccga ccggcggccg tggcggcgcc gacccacgcg gcacgactat ctggtcaagg
     1741 ccgtatgccg tgagggccgg ccggggaaga cccacaaccg cagcgttcca aatggaggaa
     1801 cctcaatgaa ttctctgcgc aagcgcatcg tcggcgggac ggccgtcggc atgaccgctc
     1861 tcactctgct ggccgtaccg gccggcgtcg ccgccaacga tggcgacatc atccgcacgg
     1921 gcaactgctc ggctggcgcg gactggaagc tcaagctgag tcccgaaaat ggccggatcg
     1981 aggtcgagtt tgaagtcgac cagaacatca acggccgcgt ctggaacgtg tccctgaagc
     2041 gcaacggcaa cacgttcttc accggccagc gcacgaccca ggcgccgagt ggctccttcg
     2101 aagtccgccg cgtgaccacc aatggcgccg gcccggacac cttcgtggct cgcgcagtcg
     2161 atcagcgcac gggccaggtc tgccgaggca gcgcgacttt ctgatcgcgc cggggaggtc
     2221 agcctccctc gatcttcctc gacgcgacga ggatgcggct ccgctgggcg gggccgcatt
     2281 ttcgtttgcc tggcctagtc gtcgaccgtc ttgagctcga cgctcgagcc ggcccaccgt
     2341 tgcacctcac cgcgcctgat cttcagggtg tgggtgcggg gaaagtcggc gtcgggccag
     2401 a
//
LOCUS       DATLCO010000269         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4205193, whole genome shotgun sequence.
ACCESSION   DATLCO010000269 DATLCO010000000
VERSION     DATLCO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4205193"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            561..2120
                     /locus_tag="VMZ33_06270"
     CDS             561..2120
                     /locus_tag="VMZ33_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUR16870.1"
                     /translation="MTTPLRLADLLAGLSMVADLGYGLPMGEATRSCLIGVALARRLG
                     LADHEVADTFYTSLLLHVGCVGFSHEMSTHFGDENTANRAGARTNFADPRDVMATLVP
                     QATRGLPPPARIKAAVFIATRGRALGRQYDTTVCEVGRETARRIGLPDGVQRALYEAK
                     EWWNGGGTPRGLKGEKIVVPARIARVAAEAALFSDLGGVQLAVHALSRRGGGMLDPAI
                     VAAFTADPSILAEADAGDPRQRILDEEPAPVVEKSLAGLPQLAAAFGDLADLKTPFTH
                     GHSKEVARLAKAAAETLGMESHAVQRLHVAAFLHDLGRVGVSNSVWEKSGPLTSADWE
                     QVRLHPYHSERILAMSEVLEPMARIVGLHHERLDGSGYHRGCRGRDLPAAARVLAAAD
                     AFQAMIETRPHRQAFTPKDAGQELARDAKAGRLDPEAVGAVIEAADQKRAVRRRDLRP
                     GGLSAREIEVLRLVSRACSNREIAEQLYISRRTAEHHVQHVYTKIGVSSRSAAALFAM
                     EHDLLPPSSEV"
     gene            2322..3077
                     /locus_tag="VMZ33_06275"
     CDS             2322..3077
                     /locus_tag="VMZ33_06275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR16871.1"
                     /translation="MDDKVALITGGTTGIGLATAHRLLAAGHVVMMASRNRERGQQVV
                     ADLCSERVGFHQVDVSDEMAVASLVDATLDRFGRLDCLFNNAGVEGTVGPMGAWTADD
                     VDAVLSVNVKGAFLCMKHAAAHMKEGSVIVNCSSLLATIPMLMAAPYAASKAALLSLT
                     RSAAAELSGSGIVVLAICPGIVDTPMMDRVSLSTGAPKSVLAEMVCPSRQVTSPDRIA
                     EVVTALLANPTSFASGSALRIGPDALEQICPGP"
BASE COUNT          507 a         1030 c         1062 g          495 t
ORIGIN      
        1 tccgaccgcg acgcttcgca ggtacccttc agtcatggag cgcgctcgag acaacgacgt
       61 tctcgcggag gacgagctgt ctgcacttct cgaacgggcg ctcgacgatg agcgcgaggg
      121 caggatcgtt cactgcgcca atgagcaaga gcttcgggac tatatgcagg gactgcaccg
      181 cgaagcttcg tgagctgggg cgtcgacttc accacgaacg cccgcgccga tctcgtcggc
      241 cttgatccag cggtcagcga ggcagtcacc gacgtgctcg tcgcttgggc acgcgatggc
      301 cctcctttag cgagccgtcg cgagctcggg ggcattgtgt ttacgagtcc acagtcgccg
      361 agcggtatct actcgggtat gccgtgaggg agtaccccca agccttcgtc gttctttggt
      421 tgcggcggaa acctggaccc atccgctaag cgcgagtcac atcgagcggc gctcgaaatg
      481 cgccgttctc cgcgccgacg agcaccggag tcgccccgtg gcgctcgtgt tcctgccgct
      541 gcggggtcaa gatgtcgacc atgacaacgc cgctccgtct cgcggacctg ctggcgggac
      601 tttccatggt ggccgacctg ggttacgggc ttcccatggg agaggcgacg cggtcgtgcc
      661 tgatcggggt tgcgctggcc cgaaggttgg gcctggccga ccatgaggtg gccgacacct
      721 tctacacgtc gcttctgctt cacgtgggat gcgtcgggtt ctcacacgag atgtccacgc
      781 acttcgggga cgagaacacc gccaacaggg cgggggcgcg gaccaacttc gccgaccccc
      841 gcgacgtcat ggcgacgctg gtcccgcagg ccacacgcgg tttgccgccg ccggcgcgga
      901 tcaaggcagc ggtattcatc gccaccagag ggcgcgccct tggccgccag tacgacacca
      961 ccgtctgcga ggtcggaaga gaaacagcgc gccgcatcgg tcttccggat ggtgtgcagc
     1021 gggccctgta cgaggcgaag gagtggtgga acggcggtgg gacacctcgc ggactgaagg
     1081 gtgagaagat cgtggtgccg gcgcgcatcg cccgtgtggc cgccgaggcc gccctcttca
     1141 gcgatctggg cggcgtccag ctggccgtcc acgccctgtc gcgtcgaggc ggcggcatgc
     1201 tcgaccccgc catcgtcgct gcgttcaccg ccgacccgtc gatcctcgcc gaggcggacg
     1261 ccggcgaccc acggcaacgg atcctcgacg aggagccggc gccggtcgtg gagaagagcc
     1321 tggccgggct gccccagctc gctgccgcct tcggcgacct ggcagacctc aagacgccgt
     1381 tcacgcacgg ccactccaag gaggtggcaa ggctggcgaa ggcggcggcc gagacgctcg
     1441 ggatggagag ccacgccgtc cagcgactcc acgtcgctgc cttcctccac gacctcgggc
     1501 gcgtcggcgt ctcgaacagc gtctgggaga agtcgggacc tttgacgtcg gccgactggg
     1561 agcaggtccg cctccatccc taccactccg agcgcatcct cgcgatgtcc gaggttctcg
     1621 agccgatggc tcgcatcgtg ggcctgcacc acgagcgact cgacggctcc gggtaccacc
     1681 gggggtgtcg gggacgtgac ctgccggctg ccgcccgtgt cctggcggcc gcggacgcgt
     1741 tccaggcgat gattgagact cggccccacc gccaggcttt cactccgaag gacgccggcc
     1801 aggagctggc tcgcgacgcg aaagcgggcc ggctcgaccc cgaggccgta ggggcggtga
     1861 ttgaagccgc cgaccagaag cgggcggtgc gccggcggga ccttcggccc ggcgggctca
     1921 gcgcccgcga gatcgaggtc ctgcgcctgg tcagcagggc gtgctcgaac cgggagatcg
     1981 ccgagcagct gtacatctcg cgccgcacgg cggagcacca cgtgcagcac gtctacacaa
     2041 aaatcggtgt gtcgagccgc tcggcggcgg ccctcttcgc catggagcac gatctcctcc
     2101 cgccgtcgag cgaggtctga ggcatggcgc ggggcagcca cctccttccg gaaattaggt
     2161 aggtcctacc gatgccgtga tcctcgtgga ggcgcgaatc tcagaccgta gtgaggaccg
     2221 agaggagcaa tccatgagcg gaagcgacac gatgacgcaa ccgaaggatc aaccggcgca
     2281 tgaggcctac tggctggctc cgggcgaggg gggccgaagt catggatgac aaggtcgcgc
     2341 tgataaccgg tggaaccact gggatcggcc tggcaaccgc tcatcggctg ctcgccgccg
     2401 gccacgtggt gatgatggca tcgagaaacc gagagcgggg ccagcaagtc gtcgccgacc
     2461 tctgcagtga gcgggtcggc tttcatcagg tcgacgtgag cgacgagatg gccgttgctt
     2521 cactcgtcga cgccaccctc gaccgcttcg gccgcctcga ctgcctcttc aacaacgcgg
     2581 gggtcgaggg caccgtcggt ccaatgggtg cctggactgc cgacgacgtg gacgccgtgc
     2641 tgagcgtgaa cgtgaaaggc gctttcctgt gtatgaagca cgcggcggcg cacatgaagg
     2701 agggctcggt gatcgtgaac tgcagctcgc tgctcgccac gatcccgatg ctcatggccg
     2761 ctccgtatgc ggcgagcaag gccgcgctct tgagcctcac ccgctcggcc gcagccgagc
     2821 tgtccggaag cgggatcgtc gtcctggcca tctgtccggg catcgtcgac acaccgatga
     2881 tggaccgcgt ctccctgtcg acgggcgccc cgaagtcggt ccttgcggag atggtctgtc
     2941 ccagccggca ggtgacgtcg ccggacagga tcgccgaggt cgtcactgca cttctcgcaa
     3001 accccacttc gttcgcgagc ggatcggcgc tgcggatcgg tcccgacgcg ctcgagcaga
     3061 tctgcccagg accgtgatcg tcgaggtcga caat
//
LOCUS       DATLCO010000270         4091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9942189, whole genome shotgun sequence.
ACCESSION   DATLCO010000270 DATLCO010000000
VERSION     DATLCO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9942189"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VMZ33_06280"
     CDS             <1..766
                     /locus_tag="VMZ33_06280"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HUR16872.1"
                     /translation="ADNLLRALTSGPVHDTVRYPVLVPNLRGMARAEEAGAGALAVFT
                     AATDAFTQRNIGMTIDESLLAAAEVFGRATELGWWKRAYVSTAFGCPFTGNVPVAAAV
                     DVATRLIDIGADEICFGDTIGVGVPSQVHEISAATLDAGIALDQIAYHFHDTRGTALA
                     NVMAGLEDGVSCFDSSTGGTGGCPYAPGAAGNLATEDLVYLLDGMAIEHGVRLGGVIE
                     AARFIAEALGKPLMSKVGQAGGWNPATGERTFAATH"
     gene            complement(732..1316)
                     /locus_tag="VMZ33_06285"
     CDS             complement(732..1316)
                     /locus_tag="VMZ33_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16873.1"
                     /translation="MPALGVVAVLAALVVLTRFDSGPPPTSREPSPPAMADVATAFPT
                     HAPEPSSFIRVPAVEPTPGVDPSASEYRDGIPTNLDGERVLRIPEALSTTPGTTILVG
                     GWHKAKPCRDRRARSCPVQTLADTDLEVVGDLSTAWVVLDRPRGIGVGARVLRATVEP
                     DPSCSITRQGECQPRLRVLRVLWLNEWQRKSALQ"
     gene            1450..1962
                     /locus_tag="VMZ33_06290"
     CDS             1450..1962
                     /locus_tag="VMZ33_06290"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HUR16874.1"
                     /translation="MDSVVLLLNSDYEPLNVCNVRRAFRLVFGEKAEVIQYNHHDVRT
                     ATEVFRAPSVIRLQHRVKRPRPRVRLSRREIFIRDKHTCQYCGAQTHDLTLDHVIPRH
                     KGGAHTWDNLVAACKHCNHRKGGRLANEVRMNLMRQPFEPRCDVYTLFTPYLNDPRNE
                     AWRDYLFIGH"
     gene            1986..3350
                     /locus_tag="VMZ33_06295"
     CDS             1986..3350
                     /locus_tag="VMZ33_06295"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HUR16875.1"
                     /translation="MQPPAPPALPALPANVERVLSTLWRSGHAAYVVGGGLRDTLLGR
                     SAQDWDVATSALPETVAGLFAASRYENRFGTVTVPGDSAGGIEITTFRRDHQYADHRR
                     PDSVTFTDSLDADLARRDFTVNAIAWGKRSAGDSAGWADPAGGRADLDARVIRAVGDP
                     TRRFDEDALRLLRAARFAAQLGFTIEPTTLDAMSATADTISHVSRERVGMELRKMLRA
                     DPPSAAFRILAETGVLAGAVPELDAQRGVPQEKATGDDLWGHCLRTVDAAAGLSDDET
                     LRLAALLHDIGKPPTFADGHFIGHDSEGAKLADALLTRLAYQRRVIDEVVVLIDNHMF
                     SYESRWSGAAIRRFIRRVGREHIAALLDLRRADNVGSGLPANAGHLDELESRIRAELE
                     AKAPLTLRELAIDGNDLVTELGIAPSPQVGELLERLLNEVLADPSRNTREALLGQVRD
                     WSAI"
     gene            3347..3862
                     /locus_tag="VMZ33_06300"
     CDS             3347..3862
                     /locus_tag="VMZ33_06300"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR16876.1"
                     /translation="MIEALLQAERLLLHGLLDQAERIYANALEQDPLNSIAVVGLARV
                     ALERGDDQLAYTQARRALEIDPENAAAQRLEARLAEILVPGVPLAPPDPPPMPRLPTS
                     APTELPGLAEDAHPSEQVVFTRNPSMAEHQAMVKQREEVPLASPHEARQPDGESQPAK
                     RAGLFRRLFGG"
     gene            3859..>4091
                     /locus_tag="VMZ33_06305"
     CDS             3859..>4091
                     /locus_tag="VMZ33_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUR16877.1"
                     /translation="MSKEGRAPMKVLVTGGAGYVGSVTVEALISAGHDVTVLDSMFRG
                     HRGAIVEGVSLLEADVADRQRVQTAVEAAGIEAV"
BASE COUNT          697 a         1401 c         1352 g          641 t
ORIGIN      
        1 tgccgacaac ctgctgcgag ctctcacgtc cggcccggtt cacgacacgg tgcgctaccc
       61 cgtgctcgtc ccgaacctac gcggcatggc ccgtgcggaa gaagccggag ccggcgcgct
      121 cgccgtcttc accgcggcaa cagacgcgtt cacgcagcgc aacatcggga tgacgatcga
      181 cgaatcactg ctcgcggccg ccgaggtttt cggccgagcc acggagctcg gctggtggaa
      241 aagagcgtac gtttcgactg cgttcggctg cccgttcacg gggaacgtgc cggttgccgc
      301 cgcagtcgac gtagccacgc ggctgatcga catcggcgct gacgagatct gcttcggcga
      361 cacgatcggc gtcggcgtcc cgagccaggt gcacgagatc agcgctgcaa cgctcgacgc
      421 cggcatcgcc ctggaccaga tcgcctatca cttccacgac acccgcggca cggcgctcgc
      481 caacgtcatg gccgggttag aggacggcgt gagctgcttc gactcgtcga ccggtgggac
      541 gggcggctgc ccctatgcac cgggcgcggc gggcaacctg gccacggagg atcttgtcta
      601 cctgctcgac gggatggcca tcgagcatgg cgtccgcctc ggaggagtca tcgaggccgc
      661 gcgattcatc gccgaggccc tgggcaagcc actcatgagc aaggtcggtc aggccggcgg
      721 ctggaaccca gctactggag agcggacttt cgctgccact cattgagcca cagcacccgg
      781 agcactcgca gccggggctg gcactcacct tgtcgcgtga tggagcagct cggatcgggc
      841 tcgaccgtgg cgcgcaggac cctggcgccg acaccgatgc cgcgcgggcg atcgagcacg
      901 acccacgcag tcgaaaggtc gcccacgacc tccagatccg tatcggcgag cgtctgcacg
      961 ggacagctcc gcgcccgtcg gtcgcgacaa ggcttggcct tgtgccaccc accgaccagg
     1021 attgtggtcc cgggcgtggt cgacagtgcc tcggggatac ggagcacccg ctcaccgtcc
     1081 aggttcgtcg gtatgccatc gcggtactcg gacgccgagg ggtcgacccc gggagtcggc
     1141 tcaacggccg gaacgcgaat gaaactgctc ggctcgggag cgtgagttgg gaaggccgtc
     1201 gccacatcgg ccatcgccgg aggtgacggc tcgcgggatg tcggcggtgg gccagagtcg
     1261 aatcgcgtca ggacgaccaa cgccgcgaga accgcgacga cgccgagagc cggcattctc
     1321 caacccacgc tggttcgggt cggcgtcaat tcaggcgttt ggctcatggg cgacatcggg
     1381 ctcattgtcg gccccctttg ctacgatgca ttcgtcgccc ggtcgagtcc cggccggctt
     1441 caggggtaaa tggatagcgt cgtactcctt ctgaactctg actacgagcc cctcaacgtc
     1501 tgcaacgtca gacgggcgtt tcgactcgtc ttcggcgaga aggcagaggt catccagtac
     1561 aaccatcacg acgtccggac cgcgacagag gttttccgcg ccccctccgt catccggcta
     1621 cagcaccggg tgaagcggcc gcggccccgt gttcgccttt cgcgtcgcga aatcttcatc
     1681 cgcgacaagc acacctgcca gtactgcggt gcgcagaccc acgacctgac gctcgaccac
     1741 gtcataccgc gccacaaggg tggggctcat acctgggaca acctcgtagc cgcgtgcaag
     1801 cactgcaacc atcgcaaggg cggtcgcctc gccaacgagg tccgcatgaa cctgatgcgc
     1861 cagccattcg agccgcgctg tgatgtctac acactattca cgccgtacct caacgaccct
     1921 cgcaacgagg cttggcgcga ctacctgttc atcgggcact gagtaggccg gacccggctc
     1981 tgaacatgca gccgccggcg ccgccggccc tgccggccct gccggccaac gtggagcgcg
     2041 tcctctcaac tctgtggagg agcggtcatg cggcctacgt cgtgggtgga ggacttcgcg
     2101 acacgctgct tggccggtcg gcgcaagact gggacgtggc cacgtccgcc ttgccggaaa
     2161 cagtggctgg actcttcgcg gccagtcgct acgagaatcg attcggcacg gtcaccgtgc
     2221 ccggcgacag cgccgggggg atcgagatca cgacattccg gcgtgaccat cagtacgccg
     2281 atcaccgacg gccggactcg gtcacgttca ccgattcgct cgacgcggac ctggcccgac
     2341 gcgacttcac ggtcaacgcc atcgcgtggg gtaagcggtc ggccggcgat tcggcggggt
     2401 gggctgatcc agctggtggg cgcgctgacc ttgacgcccg agtgatccgc gccgtcggcg
     2461 accccacgcg gagattcgac gaggacgccc tccgcctgct gcgcgccgcg cgattcgcgg
     2521 cgcagcttgg gttcaccatt gagccgacga cactcgacgc gatgtccgcc acggccgaca
     2581 cgatcagcca cgtatcacgg gagcgggtcg ggatggagct gcgcaagatg ctccgggcag
     2641 acccgccctc ggcggcgttc cggatcctcg ccgagaccgg tgtcctggcc ggtgccgtgc
     2701 ccgagctgga cgcccaacga ggcgtgcccc aggaaaaggc gacgggcgac gacctgtggg
     2761 gacattgcct gcgtacggtc gacgccgcgg ccggactgag tgacgacgag acgctgcggc
     2821 tcgctgcgct gctgcacgac attggcaagc cgccgacctt cgcggacggt cacttcatcg
     2881 gtcacgattc ggaaggtgcc aagctcgcag atgctctgct cacgcgcctc gcgtaccagc
     2941 gccgcgtgat cgacgaggtg gtcgtgctca tcgacaacca catgttcagc tacgaaagtc
     3001 ggtggagcgg cgctgcaatt cgccggttca tacggcgcgt tggccgcgag cacatcgccg
     3061 ctcttctgga tctgcggcgt gcggacaacg taggtagcgg cctgcctgcc aatgccggac
     3121 atctcgatga gctcgagtct cggatcaggg cagagcttga ggcaaaggca ccgctgaccc
     3181 tgcgcgaact tgccattgat ggcaacgacc tcgtcaccga actcggcatc gcgcccagtc
     3241 cgcaggttgg cgagctgctc gaacggctcc tgaatgaggt cctcgccgat ccgtcgcgga
     3301 acacgcgcga ggcgctgctg gggcaggtgc gcgactggtc ggcgatatga tcgaggccct
     3361 tctccaggcg gagcgactgc tgctccacgg cctgctcgac caggccgagc gcatctatgc
     3421 caacgcactg gagcaggacc cgttgaattc catcgccgtg gttggactcg cgcgcgtcgc
     3481 cctcgagcgt ggcgacgatc agctcgccta tacgcaggca cggcgcgcgt tggagatcga
     3541 cccggagaac gccgcggccc agcgcctcga ggctcgactg gcggaaatcc tcgtgcccgg
     3601 agtgcccctc gccccgcccg acccgccgcc gatgccgcgt ctgccgacct cagcgccgac
     3661 tgaactccca ggcctggccg aagacgcgca cccctctgaa caggtcgtct ttacgcgcaa
     3721 cccgtcgatg gccgaacatc aggccatggt gaagcagcgc gaagaggtgc cgctcgccag
     3781 cccacacgag gcgcgccagc cggacgggga atcccagccc gcgaaacgtg ccgggctatt
     3841 tcgacgtctc ttcggcggat gagcaaggag ggccgcgcgc ccatgaaagt gttggtcacc
     3901 ggcggcgccg gctacgtcgg cagcgtcacc gttgaggcac tgatcagcgc aggccacgac
     3961 gtgacggtgc tcgactcgat gttccgcggc catcgtgggg cgatcgtcga gggcgtcagc
     4021 ctcctggagg cggacgtcgc agaccgacag cgcgttcaaa ccgcagtcga ggctgccgga
     4081 atcgaggctg t
//
LOCUS       DATLCO010000271         2823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2740379, whole genome shotgun sequence.
ACCESSION   DATLCO010000271 DATLCO010000000
VERSION     DATLCO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740379"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /locus_tag="VMZ33_06310"
     CDS             complement(<1..2027)
                     /locus_tag="VMZ33_06310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HUR16878.1"
                     /translation="MTEGTESQRESAPVSGWAGAPTERGHFRADIEGLRAVAVVAVLL
                     FHAGVPGFAGGFIGVDVFYVISGFLITGLLRRELLRSGSVDLITFYARRARRLLPAAL
                     TVIAVTMVASVVVLPQMRIADMATDATTAALYVSNYRFALEGLDYLGDARTSPLLHYW
                     SLAVEEQFYLVWPVLMLVSARVLGVTRMGWAIATIGVASLVASVVLTEIAAPIAFFSL
                     PTRAWELAAGGLLAVAPEPLLGRVDRRWYWVIGGIGLGLIIGSAVLIDPALPFPGVIA
                     VLPVAGAALVIVSGALRGGVAALLSTAIPRWLGRISYSLYLWHWPLLILVPIAIGQDN
                     LPVRLALAGVAVLIADASTRWIEQPFRHGPHSRRRPAFVIATALVASAIVAGLSFAGG
                     RLLAPPTTPNVPQAQIEADVRDPSAVLAAPWTSGPVPEEIRRGLAWAAEDIPESRRSG
                     CAVTKLGTDPSPCVYGDETSGVTYALLGDSHMAQWLPSFTELASNSDWRVASFTKPAC
                     APVAAPTWDERLARTYDECLTWFNLVIDRIAAEEPALIVMSSARNHELALDGGHFTFE
                     ERPGEWTSAIQATVARLRTIAPVVWIADTPRHETEPLECLALRRSFDECQPLRTEALS
                     DTWSALELAAVTDAGGTVVSLNDLLCEQDACPLAFGDYLVYRDTHHLTATFA"
     gene            complement(2028..>2823)
                     /locus_tag="VMZ33_06315"
     CDS             complement(2028..>2823)
                     /locus_tag="VMZ33_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUR16879.1"
                     /translation="PAPRHRGDTRCADIPPWMPANYDPANMSDKPAYIRSRPRLPARF
                     TAGLPLTIKCESLLAVDEWLGRVVLQLGAQGRLANTLFVLTGDNGMDWGALRHYGKST
                     PHSTPLPLFMAWPGVSDSGLTQTDRLLANVDLAPTLCEIAGCVLGPYPNGHAVDGQSF
                     AGLVAPLLFSSVPARDSILLEHLGTGEAPVWRAILTDTRHQLGRQFLYITYDTGEREL
                     YRVGRTPCHQWTPGQVSDPCMLKNLARLARYGTIRRMLARELDREW"
BASE COUNT          546 a          911 c          952 g          414 t
ORIGIN      
        1 gcgaacgtgg ccgtgagatg gtgcgtgtcg cggtagacga ggtagtcacc gaatgcgagc
       61 ggacacgcgt cctgctcgca cagcaggtcg ttcaggctga ccaccgtgcc gccggcatcg
      121 gtcacggcgg cgagctccag ggccgaccag gtgtcactca gagcctcagt ccgcaacggc
      181 tggcattcgt cgaaacttcg tcgcagcgcg aggcactcga gcggctccgt ctcatggcgc
      241 ggcgtatcgg caatccaaac gaccggggcg attgtgcgca gccgcgccac agtcgcctga
      301 atggcgctcg tccactcgcc cgggcgctcc tcgaaggtga agtggccgcc gtcgagcgcg
      361 agctcatggt tgcgcgccga tgacatcacg atgagcgccg gctcctccgc agcgatccgg
      421 tcgattacga ggttgaacca cgtcaggcac tcgtcgtagg tgcgcgccag gcgctcatcc
      481 cacgtcgggg cagctacggg cgcacatgcc ggcttggtga atgaagcgac acgccagtca
      541 ctgtttgacg ccagctccgt gaagctcggc agccattgcg ccatgtgcga gtcgccgagc
      601 agggcgtaag taaccccgga tgtctcatcg ccgtagacgc atggcgacgg gtcagtaccc
      661 agcttcgtca ctgcacagcc gctgcgtcga ctctcaggga tgtcctcggc ggcccatgcc
      721 aggccgcgac ggatctcctc gggaaccggc cccgacgtcc acggcgcggc gagcactgcc
      781 gatggatcgc gaacgtcggc ctcgatctgt gcctggggca cattgggggt cgtcggcggt
      841 gccaggagcc ggccaccggc aaaggagagg ccggcgacaa tggcggaggc gacgagcgcg
      901 gtggcaatta cgaacgccgg gcgtcggcgg ctgtgcgggc cgtgccggaa cggctgttcg
      961 atccagcgcg tgctggcgtc ggcgattaag acggccacgc cggcaagcgc cagacgaacg
     1021 ggaagattgt cctggccgat cgcgatcgga acgaggatca gcagcggcca gtgccagagg
     1081 tagagcgagt atgagatgcg gccgagccag cgtggaatcg ccgtcgacag cagcgcggca
     1141 acgccgcctc gcaacgctcc gctgactatc accagcgcag cgccggcaac cggcagtacg
     1201 gcgatcaccc ccgggaaggg cagcgctgga tcgatgagaa cggcactgcc gatgatcaag
     1261 ccaaggccga tcccaccaat gacccaatac cagcggcgat cgacacggcc gaggagcggt
     1321 tccggcgcca ccgccagcag gccacccgca gccagctccc aggctcgagt cggtagcgag
     1381 aagaaggcga tgggtgccgc gatttcggtc agtacgacgc tggccacgag cgacgcgacg
     1441 ccgatcgtgg cgatcgccca tcccattcgg gtcactccaa gaacgcgagc acttacgagc
     1501 atcaagacgg gccacacgag gtagaactgc tcctccaccg caagcgacca gtagtggagc
     1561 aggggcgacg tacgcgcatc cccgaggtaa tcgaggcctt cgagggcgaa gcggtagttc
     1621 gatacgtaca gggcagccgt cgtggcgtct gtggccatgt ccgcgatgcg catctgtggc
     1681 agtacgacga cagaggccac catcgtgacg gcaatcaccg tcaacgcggc tgggaggagc
     1741 ctcctggccc gacgggcgta gaaggtgatc agatccacag agccgctgcg cagcagctca
     1801 cgacgtagaa gaccggtgat caggaagccg ctgatgacgt agaacacgtc gacgccgatg
     1861 aacccaccgg caaaaccggg cacgccggcg tgaaagagga ggacggccac gaccgcgacg
     1921 gcgcgcaggc cctcgatgtc ggcccggaaa tgaccgcgtt cagtaggcgc ccccgcccag
     1981 cccgagaccg gcgccgattc gcgctgggac tcggtgcctt cagtcactta ccactcgcgg
     2041 tcaagctcgc gagccagcat cctcctgatt gtgccgtagc gagccagcct cgccagattc
     2101 ttgagcatgc acggatcaga tacctgacca ggagtccact ggtggcacgg cgtccttccg
     2161 acgcggtaga gctcacgctc cccggtgtca tacgtgatgt agaggaactg ccgaccgagc
     2221 tggtgtctgg tgtctgtcag gatcgcccgc catacaggtg cctcgccagt gccgagatgc
     2281 tcgagcaaga tcgagtcgcg cgccgggaca gatgaaaaga gcaacggggc gacgagcccg
     2341 gcaaaacttt gaccgtccac ggcgtgaccg ttcggatagg gccccaggac gcagccagcg
     2401 atctcgcaca gcgttggcgc caggtccacg ttcgcgagga gtctgtccgt ctgcgtgagg
     2461 ccgctgtcgc tgactcccgg ccaagccatg aacagcggca aaggagtcga gtgcggcgtc
     2521 gacttgccgt agtgacgcag cgctccccag tccatcccgt tgtcgcccgt gaggacaaac
     2581 agcgtgtttg ccagtcgacc ttgagcgcca agctgcagga cgactcgacc tagccattcg
     2641 tcgaccgcaa gaagcgactc gcacttgatc gtaagcggca ggcccgcggt aaagcgcgca
     2701 ggcaatcgcg ggcgggagcg gatgtaggcc ggcttgtcgc tcatgtttgc ggggtcatag
     2761 ttcgccggca tccagggtgg aatgtccgcg cagcgcgtgt caccacgatg tcgcggcgcc
     2821 ggg
//
LOCUS       DATLCO010000272         4087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6216101, whole genome shotgun sequence.
ACCESSION   DATLCO010000272 DATLCO010000000
VERSION     DATLCO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6216101"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VMZ33_06320"
     CDS             <1..500
                     /locus_tag="VMZ33_06320"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HUR16880.1"
                     /translation="QVWGPDHHTHVKSHLGHGANWGLFAEDLPDEANRVTLSSSVTDG
                     DGIAAPEIHNSMADNARRMLDFHIERAKESMDAAGAYKIEVDRLMRYSGWHLLGTARM
                     GDDPSTSVLDRWNRAHDVANLYVVDGSCFVTSSGVNPTSTIVSIALRAADHIVETRAN
                     QAIPA"
     gene            586..1200
                     /locus_tag="VMZ33_06325"
     CDS             586..1200
                     /locus_tag="VMZ33_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16881.1"
                     /translation="MTEVGTSDSDSRTVETGAPTDLEGGMPATDGNQTAAGLTTGERT
                     VLRQIADTLIPAAHGMPSAAEIVTDDRLAFVLRARPDLLEPLKASLRPELSADVPVRL
                     EGLKEEPTNVSALQLTIVAGYYTDKRVKELIGYPGQMAIDVKSWLVPPYIEEGLIDAV
                     LARGATWRDPSTGQRVVVADAPHTYAERFTSSAPQGENDGRDGA"
     gene            1181..2671
                     /locus_tag="VMZ33_06330"
     CDS             1181..2671
                     /locus_tag="VMZ33_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUR16882.1"
                     /translation="MVATAPETKTANDTEGWLDQFRSDWDTGTGLEREVKEPATGRHL
                     LTIRESTPEDVRRACAAAAAAQPAWAEANYQDRAAVLRRAAEIYEKHRTEFGTWTQRE
                     TGAHRNKMHHEANFAAGELLAAATIPWQPYGQLVPTVQSERLSMIRRVPVGVVAAITP
                     WNSPSVLGMRVVAPALALGNAVVLKPDPQTPVCGGAVFAAIFKEAGLPDGLLQIVIGD
                     AVTGQALITDPDVDLVSFTGSTEVGRIVGAECGRLLKKVSLELGGNNPLIVLDDADLP
                     AAASAGAYSSCQVQGQVCFATGRHIVHRAVADEYVDLLAQKAKRLSVGDPYRTDVDLG
                     PIVNEQQVKHVDDIVRRSVDSGGARLVEGGTFEGLFYRPTVLADVKLDSPAWTDEIFG
                     PVAPVVTFDDDDEAVALANSTEYGLAAGVYSRSISRGLAMANRIKAGMVHVNDQSIND
                     EATIPFGGMGISGNGGRFGAEANLDAFTQWQWVTVRDEARSFGPTY"
     gene            2688..3728
                     /locus_tag="VMZ33_06335"
     CDS             2688..3728
                     /locus_tag="VMZ33_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HUR16883.1"
                     /translation="MSSITEVARRAGVSIATASRVVSAADYPVSASTRERVLDAARAL
                     DYVPNALARGLQKSYVPVVGVIVHDITDPYFAEIVRGVEDAANKTGYLVVTCSSERDA
                     QRENSYVRLLRQMRAAAVIFAGSGLDDPQLNEELPKHLAAIRSTGAAVVHLSPHALGE
                     PEIRVDNVGGIASMIAALVALGHRQIAFFDAPSMLFVARDRLAGYRRGLDDANIAYDE
                     RLVISIGFTAEDGARAVDALLERGVPVTAIACANDLLALGALQRLHALDIDVPRDVSV
                     AGFDDIPTAAMTAPSLSTVRLPLREMGRLSFAYADRVLNGETPEATLMPTEVVMRDST
                     GAPPATPLGGTQ"
     gene            3725..>4087
                     /locus_tag="VMZ33_06340"
     CDS             3725..>4087
                     /locus_tag="VMZ33_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HUR16884.1"
                     /translation="MTLNLRGIIPACVTTFDENGKFDEAQYRRYLQWLLPQGPVALAI
                     NADTGEGPHMWPEEREQVLRVAVDEAGDVPVIAGLSAMFTEQAVVEGKRARDAGAQGL
                     LMFPIPAYQGMPLDPQVPV"
BASE COUNT          709 a         1419 c         1310 g          649 t
ORIGIN      
        1 aacaggtgtg gggccccgat caccacaccc atgtcaagtc gcacctcggc cacggcgcca
       61 attggggcct cttcgctgag gacctgcccg acgaggcgaa ccgcgtgacg ctctcgtcga
      121 gcgttaccga cggcgacgga atcgcggcgc ccgagatcca caactcgatg gccgacaacg
      181 cacgacgaat gctcgacttc cacatcgagc gcgcgaagga gtcgatggac gcagccggcg
      241 cctacaagat cgaggtcgac cggctgatgc ggtacagcgg ctggcaccta cttggcacgg
      301 cgcgcatggg agacgacccc agcacgtcag tgctcgaccg ctggaaccgc gcccacgacg
      361 tcgccaacct gtacgtcgtc gacggctcgt gcttcgtgac gtcaagcggc gtcaacccga
      421 catcgaccat cgtttccatc gcactgcggg cggccgacca catcgtcgag acccgggcta
      481 accaggccat cccagcatga aattccacgc tctcccgaac atatttaggc cggtcggcgc
      541 atagttaggc cggtctgcgc atcgccccac gtatcggaga ccgtcgtgac cgaagtcggc
      601 acttcggatt ccgacagccg taccgtcgag actggtgcgc cgaccgacct cgagggcggc
      661 atgcctgcta ccgacggcaa tcaaacggcc gcaggactga cgaccggcga gcgcacggtc
      721 ctgcgccaga tcgcagacac cctgatcccg gccgcgcacg gcatgccgtc ggcggccgag
      781 atcgttaccg acgatcggct ggccttcgtc ctccgcgctc ggccggacct gcttgagccc
      841 ctgaaggctt ccttgcgtcc ggagttatca gcggacgtcc cagtcaggct cgaggggcta
      901 aaagaagagc cgaccaacgt gtcagccctc cagttgacga tcgttgccgg ttactacacg
      961 gacaagcgcg tcaaggagct gatcggctat cccggccaga tggcgattga cgtcaagtcg
     1021 tggctggttc cgccctatat cgaggagggg ctgatcgacg ctgtgctcgc tcgtggagcc
     1081 acgtggcgcg acccctcgac cggccagcgc gtcgttgtcg ctgacgcacc ccacacctac
     1141 gccgagcgct tcacatcatc ggcaccacaa ggagaaaacg atggtcgcga cggcgcctga
     1201 gacgaagacc gcgaacgaca cagaaggctg gctcgaccag ttccggtccg actgggacac
     1261 cggcaccggg ctcgagcgtg aggtcaagga gccggcgaca ggtcgccacc tgctgaccat
     1321 tcgcgagtcg acgcccgaag acgttcggcg cgcctgcgcg gccgcggctg ccgcgcaacc
     1381 tgcctgggct gaggcgaact accaggaccg tgccgcagtc ctgcggcggg ccgccgagat
     1441 ctacgagaag catcggaccg agttcgggac gtggacgcaa cgcgagaccg gcgcccatcg
     1501 caacaagatg caccacgagg cgaacttcgc cgcgggagag ttgcttgccg cagcgacgat
     1561 cccgtggcag ccctatggcc agctcgtgcc gaccgtccag tccgagcgac tttcgatgat
     1621 tcgtcgcgtg ccggtcgggg ttgtcgcggc gatcacgcca tggaattcgc cgagcgtcct
     1681 cggtatgcgc gtcgtcgccc cggcactcgc cctcggcaat gccgtcgtcc tgaagccaga
     1741 cccccagaca cccgtttgcg gcggtgcggt gttcgcggcg atcttcaagg aggcgggact
     1801 gcccgacggc ctgctccaga tcgtcatcgg tgatgccgtg accggccagg cgctcattac
     1861 cgacccagat gttgacctcg tgagcttcac cggctccacc gaggttggcc gcatcgtcgg
     1921 cgcggagtgt ggccggctcc tcaagaaggt cagcctcgag cttggtggta acaacccgct
     1981 gatcgtcctc gacgacgccg acctgccggc cgccgcgagc gcaggcgcct actcgagctg
     2041 tcaggtccag ggccaggtct gtttcgcgac cggccgccac atcgtccatc gcgcggtcgc
     2101 cgacgagtac gtcgatctcc ttgcccagaa ggccaagcgg ctcagcgtgg gcgatccgta
     2161 ccgcacggac gttgacctcg gcccgatcgt caacgagcag caggtcaagc acgtcgacga
     2221 catcgtgcgc cggtcggtcg actcgggcgg cgcacggctc gtcgaaggcg gtacgttcga
     2281 agggcttttc taccgcccca cagtcctggc agacgtcaag ctcgattcgc ccgcgtggac
     2341 ggacgagatc ttcggccccg tggcaccggt agtgacgttc gacgacgacg acgaggcggt
     2401 cgccctggcc aactcgaccg agtacggcct cgccgccggc gtctactcgc gctcgatctc
     2461 gcgtggcctc gccatggcca accgcatcaa ggccggcatg gtgcacgtca acgaccaatc
     2521 gatcaacgac gaggcgacca tccccttcgg cgggatgggg atcagcggca acggtggccg
     2581 atttggcgcg gaagccaatc tcgacgcgtt cacgcagtgg cagtgggtca cggtgcgcga
     2641 cgaggcacgg tcattcgggc caacctacta gcacgggcct cgaaaccttg agctccatca
     2701 cggaggttgc ccgccgggcg ggcgtctcca tcgccactgc ctcgcgcgtc gtctccgctg
     2761 cggactaccc ggtgagcgct tcgacgcgcg agcgggttct tgatgcggcg cgcgccctcg
     2821 actacgtgcc caacgcgctg gcgcgtggcc tgcagaagag ttacgtaccg gtggtcggag
     2881 tgatcgttca cgacatcacc gacccgtact tcgccgagat cgtcagggga gtcgaggacg
     2941 cggcaaacaa gacgggctat ctggtcgtca cctgctcatc cgaacgtgac gcccagcgcg
     3001 agaactcgta cgtgcgcctg ctccgccaga tgcgcgccgc ggcggtgatc tttgccggtt
     3061 ctggcctgga cgaccctcag ctcaacgagg agctgcccaa gcacctcgcc gcgatccgtt
     3121 ctacgggtgc agccgtcgtc cacctgagcc cgcacgccct gggcgagccg gagatccgcg
     3181 tcgacaacgt cggcggcatc gcgtcgatga tcgcggcact ggtcgcactc ggccacaggc
     3241 agattgcgtt cttcgatgcg ccctcaatgt tgttcgtggc ccgcgatcgc ctcgcgggct
     3301 accgccgtgg gctcgacgat gccaacatcg cctacgacga gcgactggtg atctctatcg
     3361 gtttcacggc ggaggacggc gcgcgcgccg tagacgcgtt gcttgagcga ggcgtacccg
     3421 tgaccgcgat cgcgtgtgcc aacgacctgc tcgcgctcgg tgcgttgcag cggctacacg
     3481 cgctggacat cgacgtgcca cgtgacgtgt cagtggctgg cttcgacgat atccccacgg
     3541 cggcgatgac agcgccgagc ctctccaccg tgcgccttcc cttgagagag atgggccgcc
     3601 tcagctttgc ctacgccgac cgagtgctca acggcgaaac ccctgaagcg acgctcatgc
     3661 cgacggaggt cgtgatgcgc gactccacgg gcgcaccccc cgccactccc cttggaggaa
     3721 cccagtgacc ctcaatctgc gcgggatcat ccccgcctgc gtgaccacgt tcgatgagaa
     3781 cggcaagttc gacgaggcgc agtaccgccg ctacctccag tggctactgc cacaggggcc
     3841 ggtcgcgttg gcgatcaacg ccgacaccgg cgagggacca cacatgtggc ccgaggagcg
     3901 cgagcaggtt ctgcgcgtgg ccgtcgacga ggctggagac gttccggtca tcgccggcct
     3961 gtcagcgatg ttcaccgagc aggccgtcgt ggaaggcaag cgtgcccgcg acgccggcgc
     4021 acagggactg ctcatgttcc cgatcccggc ctaccagggc atgccgctcg acccgcaggt
     4081 ccccgtc
//
LOCUS       DATLCO010000273         2143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8456631, whole genome shotgun sequence.
ACCESSION   DATLCO010000273 DATLCO010000000
VERSION     DATLCO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8456631"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VMZ33_06345"
     CDS             complement(<1..374)
                     /locus_tag="VMZ33_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUR16885.1"
                     /translation="MELQSEVDAPRELVFPLLATGEGLGKWLDWAEVDARVGGRVRAQ
                     LRNAVGGGEILSLQPPQHISFTWDWEGAPLGRASVVAFDAIDHGARTHVTIRHVGLPS
                     PRQVELHEQMWRYWRDRFEAAC"
     gene            complement(386..>2143)
                     /locus_tag="VMZ33_06350"
     CDS             complement(386..>2143)
                     /locus_tag="VMZ33_06350"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HUR16886.1"
                     /translation="CQVAHRSDEEFEFFPSSSETGSCGTFLTVGGFTYGPAGGSPTQR
                     PWTAVSQTVDSSRILTVVDIAEIGIRVTETDSYAPGSQAYRTDVVLQNNSQQPVEGVI
                     YRAGDCYLQGADTGYARVDGGAPACIVEPGEGQRIEQWLPITVGSHYYAGFFGTTWQI
                     VGSGEHFPDTCECTTDFKFDNGAGISWSVNLQPGQSATFAHETFFSPVGRVGLDGGQS
                     FVDSVPDPTRISLDPVVVAQSVAVAAGVVLLVPFPSALFNSTLEDNYAEVMGGVARMR
                     AWLRRRSQQARDWIRAQIAQRRTSAAAAPSQQLVEAEQPPMIAPAGVAQAEPRDVWRT
                     LPGMAGFVLLSALMYAFLDPTFGFSAQSLATFAGLAIGLFIVVLAYGIPLMLVARTRT
                     LGFSARALPASLLVAIGCVLISRFTDFQPGYLYGLIIGFFFAVGVDRKQEGRAEAIAT
                     ASSLAAALVAWVLLAFLRGGAGPSDALTSTLIQAATVTIVVAGLENAVFAMLPLRFMP
                     GSAVFDWDRRVWAALIGLGIFGFAHVLLNPSAGYMADTTRTSFITMVALLAFFALASA
                     LFWAYFRFRPARAGAGHAA"
BASE COUNT          410 a          739 c          690 g          304 t
ORIGIN      
        1 ttgcaagcgg cttcgaaacg gtcacgccag tagcgccaca tctgctcgtg cagttccacc
       61 tgacgtggcg agggcaggcc cacatggcgg atcgtcacgt gtgtccgtgc gccgtggtcg
      121 atcgcatcga acgccaccac gctcgcccgc ccgagcggcg cgccctccca gtcccaggtg
      181 aagctgatgt gctgcggcgg ctgaagcgac agaatctcgc caccgccgac tgcgttgcgc
      241 agctgcgccc gcacgcgacc gccgacgcga gcgtcgacct cagcccagtc gagccacttt
      301 ccgagcccct ccccggtggc cagcagcgga aacacgagct cgcgcggagc atcgacctcg
      361 gattggagct ccactcgatc cgcactcatg ccgcgtgccc cgccccggcg cgggccggtc
      421 ggaagcggaa atacgcccag aacagtgccg acgccagggc gaagaacgca agcagcgcca
      481 ccatcgtgat gaaggacgtg cgcgtcgtgt cggccatgta cccggccgaa gggttgagca
      541 gcacgtgcgc gaacccgaag attccgaggc cgatcaacgc cgcccacacc cgccgatccc
      601 agtcaaacac cgctgacccg ggcatgaacc tcaagggcag catcgcgaac acggcatttt
      661 ccaggccggc aaccacgatg gtcacggtag cggcctggat gagggtcgac gtcagcgcgt
      721 cgctcggccc cgcgcctccc ctcaggaacg cgagtagcac ccaggccacg agcgcggccg
      781 cgagcgacga cgccgtggcg atcgcctcag cacggccttc ctgcttgcga tcgacaccga
      841 cagcgaagaa gaacccgatg atcaggccgt acagatagcc gggctggaag tcggtgaacc
      901 gactgatgag gacacatccg atcgccacca gcaggctggc cggtaacgcc cgagccgaga
      961 acccaagggt gcgcgttcgg gcaaccaaca tcagagggat gccgtacgcc agcaccacga
     1021 tgaagaggcc gatcgccagg ccggcgaacg tcgccaagga ctgggcgcta aagccgaacg
     1081 tcgggtcgag gaacgcatac atgagcgcgc tgagtagcac gaagccggcc atgccgggga
     1141 gagtccgcca cacgtcgcgc ggctcagcct gcgcaacacc agcgggcgcg atcatcggcg
     1201 gctgctccgc ctcgacgagc tgctgcgacg gggccgcggc cgctgacgtg cgtcgctgcg
     1261 caatctgcgc gcgtatccag tcccgagcct gttgagaacg tcggcgtagc cacgcccgca
     1321 tccgcgccac gccgcccatc acctcggcgt agttgtcttc cagcgtggag ttgaacagcg
     1381 ccgacgggaa cggtacgagc aagaccacgc cggcagccac ggccacgctc tgcgcgacga
     1441 cgaccggatc gagcgagatg cgcgttggat cgggaaccga atcgacgaag ctctgtccgc
     1501 catcgagccc gacacgcccc acgggcgaga agaacgtctc gtgcgcgaag gtcgccgatt
     1561 ggccaggctg gaggttgacc gaccacgaaa tgcccgcgcc gttgtcgaac ttgaagtcgg
     1621 tggtgcactc gcaggtgtcg gggaagtgct cgcccgaccc cacgatctgc cacgtcgtcc
     1681 caaaaaaccc ggcgtagtaa tggctgccca cggttatggg tagccattgt tcgattcgct
     1741 gcccttcacc gggctcgacg atgcacgccg gcgctccgcc atccacccgc gcgtagccgg
     1801 tgtcggctcc ctggaggtag cagtcgcccg ccctataaat gacgccttcc accggctgct
     1861 gactgttgtt ctggaggacg acgtcggttc ggtaggcctg actgccgggt gcgtagctgt
     1921 ccgtctcggt cactcgaatg ccgatctcag cgatgtcgac gaccgtcaga atcctcgagg
     1981 agtcgacggt ctgtgacaca gccgtccagg gacgctgtgt gggagatccg ccggccggcc
     2041 cgtaggtgaa gcccccgacg gttaggaatg tcccgcaact gccggtttca gaggaggacg
     2101 ggaagaactc gaactcctcg tccgagcggt gcgcgacctg aca
//
LOCUS       DATLCO010000274         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4989864, whole genome shotgun sequence.
ACCESSION   DATLCO010000274 DATLCO010000000
VERSION     DATLCO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4989864"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VMZ33_06355"
     CDS             complement(<1..1227)
                     /locus_tag="VMZ33_06355"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUR16887.1"
                     /translation="MAHSSSPDGVALRLVEVAVDAVGPAGGQTLTYHLPDDLIDVTVG
                     EAVLVEYGRRKAIGVVLRVGEAPEPGRDTKPVVARVRSDGPLLARLQVELARHVATHY
                     LAPPALVVRAMLAPGMLERVERTVRPGADGGVDTQWSVRPPEAREKVERLVALTVAGR
                     DALANPPVRLGERQRALLEELGADAPELVSSARVAERHGSGTVRNLLNKGWLEATLVR
                     VERRPRQGRPDPIRGTMPTDGALSVEQAAAVRRVEELVAARQHVGILLEGQPASGKSA
                     VYAAAVAAALRAGRGALVLVPEIALAMPLLERIQHDTGHDLALLHSSLSQGERADEWR
                     RIRAGEARVVVGTRIAALAPIADLGVIVVDEEHDASYKSERTPRYQARDVALVLGRLA
                     AAPVILGSATPDVVTVG"
     gene            complement(1250..1948)
                     /locus_tag="VMZ33_06360"
     CDS             complement(1250..1948)
                     /locus_tag="VMZ33_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR16888.1"
                     /translation="MTETAVAEPAETPGRMSELVGHRPTVSILISGPSRTGKTSAANL
                     LADRYGIRNVKIGEEFRKRTGIDTGMFVKRDPAIDREMDALQADMIRTATSADPFILE
                     ARLSAFLASKERGDRDLPVVTVLFWAPEAVRMERQLRKIRRDQPERTDTLDDLVRGER
                     ERVARDLELWKSVHPELDDRNVFDPDLTDPNGKPVYDLVIDTRIGMPDVCAHYVHNWL
                     VDRGDIDSIQPEAS"
BASE COUNT          321 a          730 c          671 g          328 t
ORIGIN      
        1 cccaacggtc acgacgtcgg gcgtggcact ccccaggatc accggcgccg ccgccagccg
       61 gcccagcacc agcgcaacgt cacgtgcctg gtagcgcggg gtccgctcgg acttgtacga
      121 ggcgtcatgc tcctcgtcga caacgatgac gcccaggtca gcaatcggcg ccagcgctgc
      181 gatgcgcgtc ccgacgacga cccgtgcctc accggcgcgg atacgccgcc actcgtcggc
      241 gcgctcgcct tgggagagcg agctgtgcag cagggccagg tcgtggccag tgtcgtgttg
      301 gatgcgctcg agaagcggca tcgcaagggc gatctcgggc acgagcacga gcgcgccacg
      361 gcccgcccgc agcgcggccg ccacggccgc ggcgtacacc gcgctcttgc cgctcgccgg
      421 ctgaccctca agcaggatgc ccacgtgttg gcgagcggcg acgagctcct caactctgcg
      481 aacggccgct gcctgctcaa cgctcaaggc cccgtccgtt ggcatcgttc cgcgaattgg
      541 atccggccgg ccctgccgcg gccgccgctc cacccgaacc agcgtggcct ccagccagcc
      601 cttgttgagc aggttgcgca ccgtgcccga tccgtgacgc tcagccacgc gagcggagga
      661 caccaactcc ggcgcatctg cgcccagctc ttcgagcagc gcgcgctgcc gctcgccgag
      721 acgcacgggc gggttcgcga gcgcgtcacg gcctgccacg gtaagcgcca cgaggcgctc
      781 gaccttctcg cgcgcttcgg gtggccggac agaccactgt gtatccactc ccccgtcggc
      841 accgggtcgc acggttcgtt cgacgcgctc gagcatcccc ggcgcgagca tcgcccggac
      901 aacgagcgcc ggtggcgcca ggtagtgcgt cgcgacgtgc cgcgccagct caacctggag
      961 ccgcgccagc agcgggccgt cgctccgaac acgtgcgacc acgggcttgg tatcgcggcc
     1021 gggttccggc gcctctccga cacggagcac gacgccgatc gccttgcgcc gcccatattc
     1081 gaccagcacc gcctcgccga ccgtgacgtc gatcaggtcg tccggcaggt ggtaggtgag
     1141 tgtctgtccg ccggcagggc caaccgcgtc aaccgcaacc tcgaccaatc gaagtgcgac
     1201 gccatcaggg gaagagctgt gcgccaccgc gcgcctacgc ggcgctgcgc tacgaggctt
     1261 cgggttgaat gctgtcgatg tcgcctcgat cgaccagcca gttgtggacg tagtgggcgc
     1321 agacgtccgg cattccgatg cgtgtgtcga tgaccaggtc gtagaccggc ttgccgttgg
     1381 gatcggtcag gtcaggatcg aacacgttcc tatcgtcgag ctccggatga accgacttcc
     1441 acagctcaag gtcacgcgcc accctttcac gctcacctcg gacgaggtcg tcaagcgtgt
     1501 cggtgcgctc gggctgatcg cgtctgatct ttcgcagctg ccgctccatg cgcactgctt
     1561 ccggggccca gaagagaacg gtgacgacgg gcagatcgcg gtcccctcgc tccttgctgg
     1621 ccagaaatgc ggaaagtcgg gcctcaagga tgaatgggtc cgcagaggtc gcggtgcgga
     1681 tcatgtcggc ctgtagggca tccatctcgc ggtcgatcgc ggggtcgcgc ttgacaaaca
     1741 ttcccgtgtc gatgccggtc cgcttgcgaa actcctcgcc gatcttgacg ttgcggatgc
     1801 cgtagcggtc agccagaagg ttggccgctg aggtctttcc cgtgcgggac ggccccgaga
     1861 tcaggattga gacggtgggc ctgtggccga cgagctcgct cattcggcca ggggtctcgg
     1921 ccggctcagc gaccgccgtt tcggtcatgg gcgcggactc ctcaacgtac tcaacacctg
     1981 ctccaactcc gccggcagcg gcgcctctgc ccgaacaata gcctcttgac cgggggacac
     2041 gaactcaatc
//
LOCUS       DATLCO010000275         2296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4223788, whole genome shotgun sequence.
ACCESSION   DATLCO010000275 DATLCO010000000
VERSION     DATLCO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4223788"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VMZ33_06365"
     CDS             <1..488
                     /locus_tag="VMZ33_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16889.1"
                     /translation="LIEDKRLNPSRYAMLIASEGAIWKGGSIGEVGEADAYGHKRKTD
                     IGWALAQELHARTGEEAVNSELTYDLRSGEADSLDQMVAITFANICVDLINQGGVTGR
                     MMAVVDGKYAHVPIPEPGNPRKVDVDTMYDLDRFRPQYHNRLGRPLLLGSSVGDAVLV
                     G"
     gene            913..1830
                     /locus_tag="VMZ33_06370"
     CDS             913..1830
                     /locus_tag="VMZ33_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HUR16890.1"
                     /translation="MRGQGGGNRGFGLPGGFGLPGGSGSGSGGGGSAIPAGGGIGVVV
                     LLVIIVVVMFVMNGGLSGGNQASTDSGLQPGQQVGQDTLQQECVTGEDANERLDCRIV
                     GYVNSVQEYWGNDGGAAAAGTTYREAVTTIFDGTIDTGCGQATSEVGPFYCPNDQKVY
                     LDLAFYDVLEQLGADNAPLAQAYVIAHEYGHHIQNLTGVLERARDSETGPQSNGVRVE
                     LQADCYAGVWVGNSVATGYLEPITDAQITDALEAARAVGDDRIQQQTQGQVNQETWTH
                     GSSAQRQSWFTTGYRAADPTACDTFSGNV"
     gene            1764..>2296
                     /locus_tag="VMZ33_06375"
     CDS             1764..>2296
                     /locus_tag="VMZ33_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16891.1"
                     /translation="MVYDRLSSGGPNGLRHVQRERLATDKQRALEILALAERTEADPE
                     RLADVAREIPMALAVLINEDRSAALRMAGLLATFWEDTGRVEMGRKLTDQALEGQHRA
                     ATLDRDVSAAMPRALLAAAELAFRDGDKEEALKQAHATVRAAVLIEDYDTAARAKVML
                     SRSALREQADQRQTQAGR"
BASE COUNT          406 a          746 c          789 g          355 t
ORIGIN      
        1 tcctcatcga ggacaagcgc ctgaacccgt cgcgctacgc gatgctcatt gcgtcagagg
       61 gcgccatctg gaagggcggc tccattggcg aggtcggcga ggctgacgcg tacgggcaca
      121 aacgcaagac cgacatcggc tgggcgctcg cccaggagct gcacgcgcgg accggcgagg
      181 aggcggtgaa ctccgagctc acctacgacc ttcgcagcgg cgaggccgac tctctggacc
      241 agatggtggc aatcaccttc gccaacatct gcgtcgacct gatcaaccag ggtggcgtca
      301 ccggtcggat gatggccgtg gttgatggca agtacgcgca cgtgcccatc cccgagcccg
      361 gcaatccacg caaggtcgac gtcgacacga tgtacgacct cgaccgcttc cggccgcagt
      421 accacaaccg cctcggccgg ccgttgctgc tgggctcgtc agtgggagac gccgtcctcg
      481 tcggctgatt cgccgggctg ccccagccta gccacccccg tatgcaccac atgactgcgc
      541 atgcgggtga cgtggcggct acgggggccg gtgcgggttg aggtcggttc tggggcgcga
      601 gtgggacgtc cttatcgacc cggccgccac tcggcagtca tgtgggtgct gaggcaccag
      661 atgaggcggc gcagacagct gtcgggcagc gactatcggc cggccacacg ctgcggtagt
      721 catgtgacgc atatgagcaa gctgaacggg caggtcgtgc gcacgtcgca tgccgatgtc
      781 gctcacccgg tttatcggac ctgaaaggtt cccgttgggg gtcggctatg gccgcgccgg
      841 ggtcagatcg ggcaccatat cggtatgaca tttcgacggg gggctcgact taatcctgga
      901 caagtgcgcg acatgcgcgg tcagggtggc ggcaaccggg gcttcggcct gccgggcggc
      961 ttcggccttc cggggggatc cggcagcggc tccggtggcg gaggttcagc gatcccggcc
     1021 ggtggtggca tcggcgtggt cgttctcctc gtgatcatcg tggtcgtcat gttcgtcatg
     1081 aacggcggcc tgagcggtgg taaccaggcc tcgacggact ccggcctgca gcccggccaa
     1141 caggtcgggc aggacacgct ccagcaggag tgtgtcacgg gcgaagatgc gaacgagcgg
     1201 cttgactgcc ggatcgttgg ctacgtcaac agcgtgcagg agtattgggg taacgacggc
     1261 ggcgccgctg cggccggcac gacttaccgc gaggcggtca cgacgatctt cgacggcacc
     1321 attgacaccg gctgcggcca ggcaacgagc gaagtcgggc cgttctactg ccccaatgac
     1381 cagaaggtct acctggacct cgcgttctac gacgtacttg agcagctggg cgccgacaac
     1441 gcaccactcg cccaggcgta cgtcatcgcc catgagtacg gacaccacat ccagaacttg
     1501 accggcgtcc tcgagcgggc gcgcgacagc gagaccggcc cgcagagcaa cggcgtccgc
     1561 gtcgagctcc aggccgactg ctacgccggc gtttgggtcg gcaactctgt ggccacgggc
     1621 tatctggagc cgatcacgga cgcacagatc acggacgccc tcgaggcggc acgcgccgtt
     1681 ggcgacgacc gaatccagca gcagacgcag ggtcaggtca accaggagac ctggacgcac
     1741 gggtcgtcgg ctcagcggca gtcgtggttt acgaccggct atcgagcggc ggacccaacg
     1801 gcctgcgaca cgttcagcgg gaacgtctag ccacggacaa gcaacgcgcg ctggaaattc
     1861 tggcgctggc cgagcgcact gaggcagacc ctgagcgtct ggccgacgtc gcgcgcgaga
     1921 tccccatggc cctcgccgta ctcatcaatg aggatcgctc ggccgccctg cgcatggccg
     1981 gattgctagc gacattctgg gaggacaccg gccgagtaga gatgggccgc aagctgaccg
     2041 accaggcgct cgagggccaa catcgagccg cgaccctgga tcgcgatgtt tcggcggcca
     2101 tgccgcgtgc gctcctggcg gcggccgagc tggcttttcg cgatggtgac aaggaagagg
     2161 cactcaagca ggcacacgcc acggttcgtg ccgcggtact gatcgaggat tacgacacgg
     2221 ccgcccgagc caaggtcatg ctctcccgct ccgcgctacg cgagcaggcc gaccagcgtc
     2281 aaacccaggc cggccg
//
LOCUS       DATLCO010000276         4121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2010497, whole genome shotgun sequence.
ACCESSION   DATLCO010000276 DATLCO010000000
VERSION     DATLCO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010497"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /gene="hutU"
                     /locus_tag="VMZ33_06380"
     CDS             <1..1201
                     /gene="hutU"
                     /locus_tag="VMZ33_06380"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HUR16892.1"
                     /translation="GGTLRGRIVLTAGLGGMGGAQPLAVTMNEGVALVIEVNESTARR
                     RMDQGFVDRITYDLDEGLAWARAAAAEGRAESIALVGNAAELEPELVRRGEGFDVLTD
                     QTSAHDALNGYIPAGLDVRDASALRDRDTQRYIAAAMESMATQVRAMLALQGAGAVTF
                     DYGNNIRAQAMTAGVENAFDFPGFVPAFIRPLFAEGKGPFRWAALSGDPADILRTDEA
                     VLELFPEDTGLRRWIRMAQQKVPFQGLPARICWLGYGERALAGARFNELVASGEVSAP
                     LVIGRDHLDSGSVASPNRETEGMRDGSDAIADWPLLNALVNTAAGASWVSIHHGGGVG
                     IGYSQHAGMVIVADGTPLAAQKLERVLTTDPGMGVIRHADAGYERAIEVAHERGVRIP
                     MLDDTPN"
     gene            complement(1288..2043)
                     /locus_tag="VMZ33_06385"
     CDS             complement(1288..2043)
                     /locus_tag="VMZ33_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16893.1"
                     /translation="MADDIRDLVRGINREQARQFEERLRQELSGRDPAWLIDQLVALA
                     LADRERRLGTADEADDSPEEPAERALRADRVRKLRLDTDKVRAFAARHESVDRQRLMA
                     SGLLAADAPEKGTDLLTSAHRGAAGDALLDEAKDVLFGLLFGDETTETRLARVQQELL
                     TVAVPRAKADALDFMRASTELAAAGTWQDPESVSNDERAENVLIEVEFGETADEVVGA
                     GIVAALSLINNLEINEQVLYARMINVEESTLIS"
     gene            complement(2064..3389)
                     /locus_tag="VMZ33_06390"
     CDS             complement(2064..3389)
                     /locus_tag="VMZ33_06390"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16894.1"
                     /translation="MSSPSRSPLAVRDFRLLWFGESVSVLGDQFALVALPWLALLLTG
                     SPLALGTVLALMAVPRAVLMIVGGAYVDRLSPRTVMLVSNGVRLVAVALLGGIVLAGA
                     AQLWMLYIFALVFGIADAFFYPAHTAIVPDLVSSDQLQRANGIAQGTAQLSVLIGPAL
                     AGIAIAGLSSAQAGPGLTGVGVALLIDALTFLVSLGALALIKPRPAHPGANDTSVVTQ
                     IREGIAFAWQAKALRVVMLLSMLLNFLITGPFEVGIPVLAFSRLPEGAAAFGLMNAGF
                     GAGSVLGLIAATMLPALPKAHFGTVMLLLIASTGVAIVAMAFATSTLHAIAVAVVIGS
                     ILGYTNVSFLTWLQRRIPRALMGRVMSLLMFSSIALVPISMAIAGALVEISLEGLFVV
                     AGVGMALITLLALFSRNVRQMGLEPILDEKADPDAADAQVASPRVPAAV"
     gene            complement(3399..3590)
                     /locus_tag="VMZ33_06395"
     CDS             complement(3399..3590)
                     /locus_tag="VMZ33_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16895.1"
                     /translation="MFVVTTDLDEPYVPFVTGRHSNGRMHEMRRRESARVGAPRRAVG
                     KALIRAGLAVAGLDSAGSR"
     gene            complement(3623..3991)
                     /locus_tag="VMZ33_06400"
     CDS             complement(3623..3991)
                     /locus_tag="VMZ33_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16896.1"
                     /translation="MPDVEKILRLVAEGTLTAEEADEILSALRPAEQPASPPAAPQGT
                     STDQKQSRHLRIEVTERGRQVVNLRVPVNVAAWASHLLPGLSDENAQRIRSAIDTGSR
                     GPILEVNDDDGATRVLIVSE"
BASE COUNT          688 a         1342 c         1449 g          642 t
ORIGIN      
        1 cgggggcacc ctgcgcggac ggatcgtcct gacagcgggc ctcggcggca tgggtggcgc
       61 ccagccactg gcagtcacga tgaacgaggg cgttgcgctc gtaattgagg tcaacgagtc
      121 gacggcacgg cggcgaatgg accagggctt tgttgatcgg atcacgtacg acctcgacga
      181 agggctggcc tgggcgcgcg ctgcagcggc cgagggtcgc gcggagtcga tcgccctggt
      241 cggcaacgcc gccgagctgg aacctgagct tgtccgccgc ggggaggggt tcgacgtgct
      301 gaccgaccag acgtccgcgc acgatgcgct caacggctac atcccggccg ggttggacgt
      361 tcgcgatgcc tctgcgctgc gcgatcgcga tacacagcga tacatcgccg cggccatgga
      421 atcgatggca acgcaggtgc gggcgatgct cgcgctccag ggagcgggcg cggtgacgtt
      481 cgactacggc aacaacatcc gcgcgcaggc catgacggca ggtgtcgaga atgcgttcga
      541 cttcccgggg tttgtgccgg cattcatccg gccactcttc gccgagggca aggggccttt
      601 ccgctgggca gcgctatcag gcgatccggc agacatcctt cggaccgacg aggccgtgct
      661 cgagctgttc cccgaggaca ctgggctgcg ccggtggatc cgcatggccc agcagaaggt
      721 tccgttccag ggtttaccgg cccggatctg ctggctcggc tacggcgagc gggcacttgc
      781 cggggcgcgc ttcaacgagc tcgtcgcatc aggggaagtg tccgcgcccc tcgttatcgg
      841 ccgcgaccac ctcgattccg gctcggtggc ctcgcccaac cgcgagacgg agggcatgcg
      901 cgacggctcg gacgccattg ccgactggcc tctcctcaat gcgctggtca acaccgccgc
      961 cggtgccagc tgggtctcga tccaccatgg tggcggcgtc ggcatcggct actcgcagca
     1021 cgcggggatg gtcatcgtcg cagacggcac gccgctcgca gcccaaaagc tcgagcgggt
     1081 tctaacgact gacccgggca tgggcgtcat ccgtcacgcc gatgccggct acgaacgggc
     1141 catcgaggtg gcacacgagc gcggggtacg aatcccgatg ctcgatgaca ccccgaacta
     1201 gggtcgcctc agccgccaac gccttggcag tcgtcgccct gctgccacgg gctgcgcggc
     1261 gatcgatcca gtgatggtcc gcccgcgcta ggagatgagc gtcgactcct cgacgttgat
     1321 catccgcgca tagagcacct gctcgttgat ctccaggttg ttgatcaacg acagtgccgc
     1381 cacgatcccc gcgcccacga cctcgtcggc ggtttcgccg aactcgacct caatcagcac
     1441 gttctcggcg cgctcgtcgt tggaaacgct ctcgggatcc tgccacgtgc cggctgcggc
     1501 cagctcggtt gaggcgcgca tgaagtcgag cgcatcggcc ttggcacgcg gcaccgcaac
     1561 ggtgagcagc tcctgctgga cacgggcgag acgagtctcg gtcgtctcgt cgccgaagag
     1621 caggccgaac aggacatcct tggcttcgtc gagcagcgca tccccggccg cccctcggtg
     1681 ggcagaggtc aagaggtccg tgcccttctc cggagcatct gcagcgagga ggccactggc
     1741 catcagccgc tgacggtcga cgctttcgtg tcttgctgcg aacgctcgca ccttgtcggt
     1801 gtcgagtcgg agcttgcgca ccctgtccgc gcggagagcc cgctcggccg gctcctcagg
     1861 cgaatcgtcg gcttcatcgg ccgtgccgag ccgtcgttcg cggtcggcca gcgccagcgc
     1921 aacgagctga tcgatgagcc aggcgggatc cctgccgctc agctcctggc gcagccgctc
     1981 ctcgaactgc cgtgcctgtt cgcgattgat gccgcggacc aggtcgcgga tgtcatcggc
     2041 cacgcccaga gcgtagcgct gcgtcaaacc gcggcgggaa ctcgtggact tgcgacctgg
     2101 gcatccgctg cgtcgggatc ggccttctcg tcaaggatcg gctcgagtcc catctgtcga
     2161 acgtttcggg agaacagcgc gagaagggtg atcagcgcca tgccaacgcc ggcgacgacg
     2221 aacaggcctt cgagactgat ctccaccaaa gccccggcga tggccatcga tatcggcacg
     2281 agcgcgatcg aagagaacat cagcaggctc atcacgcggc ccatcaacgc tcgcgggatc
     2341 cggcgctgga gccaggtcag gaacgaaacg ttcgtgtagc cgaggatgga cccgatgacc
     2401 actgcgacag cgatcgcgtg gagggtgctg gtcgcgaacg ccatggccac gatggcgacg
     2461 ccggtcgagg cgatgagcag cagcatgacg gtgccgaagt gtgccttagg caatgccggg
     2521 agcatggttg cggcgatgag gcccagaacc gagccggcgc cgaaccccgc gttcatgagc
     2581 ccgaacgccg ccgcgccctc aggcaagcgc gagaaggcga gcaccgggat gccgacctcg
     2641 aacgggcccg tgatcaggaa gttgagcagc atgctcaaga gcatcacgac gcgaagggcc
     2701 ttggcctgcc aggcaaaggc gatcccctcc cggatctgag tgacgacgga tgtgtcattg
     2761 gcgcccgggt gcgccggtcg gggcttgatc agcgccaagg cgccgagtga gacgaggaag
     2821 gtcagcgcgt cgatcagcag cgcgacgcca acgccggtca ggcctgggcc ggcctgagcg
     2881 ctgctcagtc cggcgatcgc gatgccggcc agcgccggtc cgatcagaac tgacagttgc
     2941 gccgtgccct gggcgatgcc gtttgcccgc tgcagctgat cgctgctcac gaggtccggg
     3001 acgatcgcgg tgtgggcggg gtagaagaat gcgtcggcga tgccgaacac cagcgcgaag
     3061 atgtagagca tccacagctg ggcggcgccc gccaggacga tgccgccgag caacgcaacg
     3121 gcgacgagcc gcacgccgtt ggagacgagc atcaccgtcc gcggcgacag ccggtcgacg
     3181 tacgcgccgc cgacgatcat caacacagca cgcggcacgg ccatcagcgc caagaccgtg
     3241 ccgagcgcca gcgggctgcc ggtcagcaac agcgccagcc agggcagggc cacgagcgcg
     3301 aactggtcgc ccaggactga gacggactcg ccgaaccaca ggaggcggaa gtcgcgtacc
     3361 gcgagtggcg agcgtgacgg ggagctcatc ggggttgcct agcggcttcc cgcgctatcg
     3421 agaccggcga cggccaggcc ggcgcggatg agggctttgc cgacggcacg tcgcggagct
     3481 ccgactcgcg ctgactcgcg gcggcgcatc tcgtgcatcc ggccgtttga atggcgaccg
     3541 gtgacgaatg gcacgtacgg ctcgtccagg tcggtggtga ctacgaacat tgaatggttc
     3601 ctttgtgtga ccgaattggc ggttattcgc tgacgatcag gacgcgagtc gcgccgtcgt
     3661 cgtcgttgac ttcgaggatc ggcccgcgcg agccggtgtc gatcgcggag cggatgcgct
     3721 gcgcgttctc atcggacagg ccggggagaa ggtggctggc ccacgcggcg acgttgaccg
     3781 gaactcgcag gttcacgacc tgacgaccgc gctcggtcac ctcgatccgg aggtgacgcg
     3841 actgcttctg gtcggttgag gtcccctgcg gagcagccgg gggagaggcc ggctgctcgg
     3901 cggggcgtag tgcggacagg atctcgtcag cctcctcggc cgtgagcgtg ccctcggcga
     3961 cgaggcgcag gatcttttcg acgtcaggca tgtggcgctc cttcgaggcg tcgcgaggcc
     4021 tcttcgatgt cgatctcgcc gcgctcgagg gcgcggagga tgtccagcga gtcctcgcgg
     4081 ctgggtgttg cgggtgacgg cggaaccggt ggcggtggtg g
//
LOCUS       DATLCO010000277         2700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7712231, whole genome shotgun sequence.
ACCESSION   DATLCO010000277 DATLCO010000000
VERSION     DATLCO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7712231"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1077)
                     /locus_tag="VMZ33_06405"
     CDS             complement(85..1077)
                     /locus_tag="VMZ33_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16897.1"
                     /translation="MQEQVPAAAKPPTAANRLSDVAARYQGLIVYLGFVLIVVFFSIV
                     LRDKGFLTPDNLLNILRQTTFVSIMAIGMTFALSAGEIDLSIGSTVALSALVTAVFLR
                     DFGIVLAIPVGLVVGLVIGLINGVITTKLRIPSFLVTLGMLGIISGLARQLTNLQSVP
                     VTDDLYTYVFGSGSMGPVPTLIIWTALILFVGHIVYRRTTFGRQVLATGGNRTAARYS
                     GIATDRIRIAVLVISSVTAAFAGMLFAGRLHGARYTLGEEELLTVIAAVVIGGTSLFG
                     GRGSVIGAVIGSLIMGVLTNGLILMGLTVSEQMIARGAIIILAVALSLREAKDS"
     gene            complement(1153..2388)
                     /locus_tag="VMZ33_06410"
     CDS             complement(1153..2388)
                     /locus_tag="VMZ33_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUR16898.1"
                     /translation="MDMKRAFGLVCATALIAVACGGPAVTQRASTGASQAPASTGASS
                     APSTAASTEPSVAASAQATPGVDLGHCSDAAADLSATSTGPNGEESTSASDIPDLTDE
                     QVAAANAAGYKWAYLPSGASTWFNAVGEGAKAEAERLGLTLTVTADSNFDPAKQAADV
                     ESAMATDPDIIVTLPVDPTQGAEAFRPAVDAGVKLVFNDNGVDGFTAGEEYVAIVTGD
                     HFGMGDAAADLMSQALPDGGPIGVIFHDADFYVTNNRDCRFVAAVEQNYPELEIVATS
                     GMTEEGATESITSAMLTQHPEIKGIYVTWSAAATGALAALRGADRSDVKVVVHDLDAA
                     NDLDMAQNGNHFGAAADRPYEIGQMQIRTAVLSLLGQDVPGFITVPVVTETRDTIVEA
                     WHDSLNAEPPQEILDALAE"
BASE COUNT          470 a          851 c          921 g          458 t
ORIGIN      
        1 cggacgccgc gcgcctcgag aaccggcacg cgaccgtctg ccgcgacctg agcgccgacc
       61 tgtggctggc tggcgagcga caagctagct gtccttcgcc tcgcgcaggc tgagcgcgac
      121 ggcaaggatg atgatggcgc cacgcgcgat catctgttcg gacacggtca ggcccatcag
      181 gatgagcccg ttcgtgagga cgcccatgat cagcgagccg atcacggccc cgatcactga
      241 cccgcggccg ccgaagaggc tcgtcccgcc gataacgaca gcggcaatga cggtcagcag
      301 ctcttcctcg ccgagggtgt accgcgcgcc gtggagacgg cctgcgaaaa gcatgcccgc
      361 gaacgctgcg gtaactgatg agatgaccag cacagcaatc cggattcggt ccgttgcgat
      421 gcccgagtag cgtgccgcgg tgcgattgcc cccggtcgcg aggacttgcc ggccaaaggt
      481 cgtgcggcgg tacacgatgt gaccgacgaa gaggatgaga gccgtccaga taatgagcgt
      541 gggaaccggg cccatgctgc cggacccgaa cacatacgtg tacaggtcat cggtcacagg
      601 gacggattgc aggttggtca gctggcgcgc gaggccgctg atgatgccca gcatcccgag
      661 cgtgaccagg aatgacggga tgcgcagctt cgttgttatg accccgttga tgaggccgat
      721 cacgagaccg accaccagtc caaccgggat ggccagcacg atcccgaaat cacgcaggaa
      781 gacagccgtt acgagggcgc tgagagccac ggtagaaccg atcgacaggt cgatctcacc
      841 cgccgacagg gcgaacgtca tcccgatcgc catgatcgag acgaaggtcg tttggcggag
      901 gatgttgagc aggttgtccg gcgtaaggaa gcccttgtca cgcaggacga tgctgaagaa
      961 gacgacgatc aggacaaagc ccaggtagac gatgagtccc tggtaccgag cggcgacgtc
     1021 ggacaggcga ttagcggcgg tcgggggctt ggctgccgcc ggtacttgct cttgcatcta
     1081 gctgagcatt acctcaaatg agtgagccgg gcaacacggc gttccgcgtt gcccggctcg
     1141 ttggatggtt ccttactcgg cgagcgcgtc gaggatctcc tgcggcggct cggcattgag
     1201 gctgtcgtgc cacgcctcga cgatcgtgtc gcgcgtttcg gtcaccaccg gaacggtgat
     1261 gaagccgggc acgtcctgtc cgagcagcga caacaccgcc gtgcggatct gcatctggcc
     1321 aatctcgtac ggacggtctg cggcagcacc gaagtggttg ccgttctggg ccatgtccag
     1381 atcgttggcc gcatccaggt cgtggacgac gaccttgaca tcggaccgat cggcaccgcg
     1441 cagtgcggcc agcgcgcctg tggcggctgc gctccatgtg acgtagatgc ccttgatctc
     1501 gggatgctgc gtcagcatcg ccgacgtgat gctttcggtc gcgccttcct cggtcatccc
     1561 ggaggtggcc acgatctcga gctctgggta gttctgctct accgctgcaa caaaccggca
     1621 gtcgcggttg ttggtcacgt agaagtccgc atcgtggaag atgacgccga tcgggccgcc
     1681 gtcgggcagc gcctggctca tcagatcggc ggcagcgtcg cccatcccga agtggtcgcc
     1741 ggtgacgatc gcgacgtact cctcgccggc agtgaaaccg tcgacgccgt tgtcgttgaa
     1801 tacgagcttg acgccggcat caaccgccgg acggaaggcc tccgcgccct gggtcggatc
     1861 gaccgggagc gtcacgatga tgtcggggtc ggtcgccatg gcgctttcga cgtccgcggc
     1921 ctgcttggcc gggtcgaagt tcgaatcggc cgtgacggtc aacgtcaggc caaggcgctc
     1981 tgcctctgcc ttggcgccct cgcccacggc gttgaaccag gtcgatgcgc cggacggcag
     2041 gtatgcccac ttgtagccag ccgcattggc tgctgccacc tgctcgtcgg tcaggtccgg
     2101 gatatcggag gccgacgtcg attcctcgcc attcgggccg gtggaggttg ccgagaggtc
     2161 ggccgccgcg tccgagcagt ggccgaggtc aacacccggc gtggcttggg cgctcgctgc
     2221 cacggaaggc tcggtgctcg ccgctgttga tggtgcgctc gaggcgccag tgctcgcggg
     2281 cgcctggctc gcgccggtcg atgcccgctg cgtgactgcc gggccgccgc acgcaacagc
     2341 gataagcgcc gtcgcacaca cgagcccgaa tgctcgcttc atgtccattg attgatcctc
     2401 ctcagggttg gtggcggaag actattgccg tggggccgac acgagccggc cggacggccg
     2461 ctcggtgata acaggggtgg tattgcgaaa ctcgccggcc tcgaaacccg agagctcagc
     2521 gaggcgcgcg cagagaagct gaacggggac gatgtctgtc agcagtgccg cgatccccgg
     2581 acgcggcgtg gtggcaatgt gagtgacggt gcccgctgac gcgtcgcctt cgaggcgccc
     2641 gccgataacg actacgcggc cgtttgcccg agtgatctcg gcgaccgcca gtcacgggtc
//
LOCUS       DATLCO010000278         4151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2019007, whole genome shotgun sequence.
ACCESSION   DATLCO010000278 DATLCO010000000
VERSION     DATLCO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019007"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1065)
                     /locus_tag="VMZ33_06415"
     CDS             complement(136..1065)
                     /locus_tag="VMZ33_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HUR16899.1"
                     /translation="MTDTSFDLVSWTAPQMGGRTEVAIATEQHRRLVAGVAARRAGQR
                     IAAWADRLTRFDERSDLSLLNASAETTVAVRPTLGATLCWAATASERTDGIVDPTLLH
                     ARVAAETGSTSSPLPDASGWAIRAVGRTTLVDRTQPTRFDLDGIAKGWLADRAVDLLS
                     EWSAVSVDADGDICVRLDAGMEWLIDIADPRASGAHPLATLRLTGGQSWSQTYGVATS
                     GTTVHRWQIGDGHATHHLIDPRTGVPADTDVVQATVLAPTAREAEALAKAAIIHGSND
                     ALLFLTRSAALTAILLLETGDVAAMSGIDRWLA"
     gene            complement(1067..1558)
                     /locus_tag="VMZ33_06420"
     CDS             complement(1067..1558)
                     /locus_tag="VMZ33_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16900.1"
                     /translation="MNASAPAARFAKPDPRPMRWAFGASAVAAVSILTVGFVKPDFAS
                     TDDQAVVNDSVAEQAAGATKQSSRARVNRVTRYVYLERGERAPRGATVIDADQAPGRR
                     QQPRGDQPDDPRAATNTRATNTRPTNTRPTNNQRQNNDRPAADPQPRPEQPPRTTTHQ
                     SGG"
     gene            complement(1705..2214)
                     /locus_tag="VMZ33_06425"
     CDS             complement(1705..2214)
                     /locus_tag="VMZ33_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16901.1"
                     /translation="MVHKLALILGGAGVAGVFALALVLGGSPFALPASAESGVAALAS
                     GSAPTNAPEPTATDGRKTRTVVDTVFVRPAPTAEATADPTAAPSADPTRRPAANPTAQ
                     PKPDSNSGRNEGRADNNDDSVRSGSNRGSDHDDSFDDDDSDHDHDDEHDEHHSGHGRG
                     DDDEREDHD"
     gene            2390..3061
                     /locus_tag="VMZ33_06430"
     CDS             2390..3061
                     /locus_tag="VMZ33_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR16902.1"
                     /translation="MTRLLLVEDEPRIASAIKRVLLAEHYTVDAAEDGETALAFLGEH
                     AYDVVILDRLLPGISGDDVLRTMRGRANHTPVLMLTALSALEHRVEGLDAGADDYLPK
                     PFAFSELLARLRALTRRPREVATERLSIGGIMLEPDRHQVSVDGKSELLSAREYALLG
                     YLIRNAGQVVTRQQILDAVWGAEPDVYSNVVDLYVHYLRRKLAALGREEVLHTVRGVG
                     YALRT"
     gene            3045..>4151
                     /locus_tag="VMZ33_06435"
     CDS             3045..>4151
                     /locus_tag="VMZ33_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HUR16903.1"
                     /translation="MRFGRKRGDGFDAPITAVDRVVRRTSVGLAVVTLVLIGALLAGV
                     GVVTAAVALRASSDQVDRTLKQAADSRIQQLQLQAEQVELPTPTPSASPTSTSSDDDE
                     PTARPTPSPTRTRTPRPTQTARQTVRPTRTRTPEATRTNDNSGPGNGDGTPRPARTDN
                     SGSLSRDAAIGAFAQLRLFPTPAPASTTTARPTPSASFALPGLGDRPPESADTFFLLL
                     NTAGDVISNPQGVSLDGLPDEAAVVTAIITGEDWRTTTVRGVRVRLLTQPVRDPDGNP
                     VGVLQSGFVLTLQDQQTNQLLTTIALACLVGLTGAALITLFITRRAMRPIRSAFDAER
                     RFVASASHELRTPAAVVRANAEILEREDLVKPEGK"
BASE COUNT          634 a         1399 c         1438 g          680 t
ORIGIN      
        1 tctctgcgct ggcgccgaac cagccggcga cggtctgcgc tgcgcccggc gcggttgcat
       61 aaagcacggc gccgataacc agcaggaaga gcgcccaacg cagcagccgc gttcgcgggt
      121 cgagcccgcg gtacttcagg caagccatcg gtcgatcccg gacatggccg ccacgtcacc
      181 cgtttcgagc aagaggatgg ccgtcagcgc cgccgagcga gtcaggaaaa gaagcgcgtc
      241 gtttgacccg tggatgatcg cggccttggc cagtgcctcg gcctcgcgcg cggtgggtgc
      301 cagcacggtc gcctgcacga cgtccgtgtc ggcaggcacg ccagtccgag ggtcgatgag
      361 atggtgtgtg gcatgtccat caccgatctg ccagcgatgg accgtcgtgc ccgaagtcgc
      421 gaccccgtag gtttgcgacc agctctggcc gccggtgagt cgcagggtcg ccagcgggtg
      481 cgcgcctgag gcgcgcgggt cggcaatgtc gataagccac tccatgccgg cgtcgagccg
      541 cacgcagatg tcgccgtcgg cgtcgaccga tacggccgac cattccgaca ggagatcaac
      601 cgcccgatca gccagccagc ccttggcgat gccgtcgagg tcgaaccgcg ttggctgcgt
      661 ccgatcgacc agagttgtcc ggccgactgc tcggatggcc cagccgctgg cgtcaggaag
      721 agggctggac gtggagcctg tctcggccgc gacgcgtgca tggagcaggg tcggatcaac
      781 gatgccgtcg gtgcgctcgg aggcagtcgc ggcccaacaa agggtggcgc cgagcgtggg
      841 acgcacagcc acggtggttt cggccgaggc attgagcagg ctcaggtccg atcgctcgtc
      901 gaagcgagtc aaccggtcag cccacgctgc tatgcgttgg ccggcgcgcc gcgcggccac
      961 acccgccacg aggcgccgat gttgttccgt agcgatcgcg acctcggttc gcccacccat
     1021 ctgcggcgcg gtccaggaca cgaggtcgaa agatgtgtct gtcacgtcag ccgcctgact
     1081 ggtgtgtggt cgtccgtggc ggctgctcgg gacgaggctg cgggtcggct gcaggtcgat
     1141 cgttgttctg tcgctggttg ttggtcggcc gcgtgttggt cggccgcgtg ttggtcgccc
     1201 gcgtgttggt cgcggcccgg gggtcatccg gctggtcgcc gcgaggctgc tggcgtcggc
     1261 cgggcgcctg atcggcgtcg atgaccgttg ctccgcgagg cgcacgctcg ccgcgctcca
     1321 ggtacacgta gcgcgtcacg cggttgacgc gagcccggga gctttgcttg gtggcgccgg
     1381 ccgcctgctc agcgacggag tcgttgacga ctgcctggtc gtccgtgctg gcgaagtcgg
     1441 gtttcacgaa accaacggtc aggatgctga ccgccgcgac tgcgctggca ccgaacgccc
     1501 aacgcatcgg gcgcggatca ggcttggcga atcgtgccgc gggtgcggag gcgttcaatg
     1561 gtgcccctgg agcggcgatg ctcatgggcg aggcggcgat tggtgccggg gctgcgaccg
     1621 gagcggcgcc cgcgacgtcc ttggtcggta gtggacgacc tgcagtcaca gaccggcgtg
     1681 gcgggagggg cggcaggtcg gccatcagtc gtggtcctcc cgttcgtcat catcaccgcg
     1741 gccgtggccg gagtgatgct cgtcgtgctc gtcgtcgtgg tcgtgatccg agtcgtcgtc
     1801 gtcgaacgag tcgtcgtgat cggaccctcg gtttgagccg gagcgaaccg agtcgtcgtt
     1861 gttgtcagca cggccttcgt tgcgaccact gtttgaatcg ggcttcggtt gcgcggtcgg
     1921 gttcgcggcc ggccggcgag tcgggtctgc gctgggagcg gcggttgggt cagccgtcgc
     1981 ttcggcagtt ggggccggtc tgacgaacac ggtgtcgacc actgttcggg tcttccgccc
     2041 gtcggtagcg gtcggctcgg gggcattggt cggcgcagaa ccgctggcga gtgccgcgac
     2101 tccggattcg gccgatgcgg gcagggcaaa cggcgacccg ccaagtacga gcgccagggc
     2161 gaagacaccg gcaacgccgg cgccaccgag tattagtgcg agcttgtgga ccattcgtgt
     2221 ccctcccgat atgtggactg agaacacggg aaagactcgc aacggcgttt gaggccatcc
     2281 tttgaccagg attggagttc gcacacgctg gcgccaccct cgatgagcgt tctcaaagaa
     2341 acgcgcctca accgttgcgc gcgcgcgtga accgggggtt aatgcagcca tgacacgcct
     2401 gctactggtc gaggacgagc cacgcatcgc atctgcgatc aagcgcgtgc tcctcgccga
     2461 gcactacaca gtcgacgcag ccgaggacgg cgagacggca ctcgcgttcc tgggcgagca
     2521 tgcctacgac gtcgtgatcc tcgaccgtct gctgcccggg atcagtggcg acgacgtgct
     2581 gcggacgatg cgtggccgcg cgaaccacac tccggtgctg atgctgaccg cactctccgc
     2641 cctggagcat cgcgtcgagg ggctcgacgc cggcgccgac gactacctgc ccaagccatt
     2701 cgccttttcg gagcttctgg cccggctccg ggcactgacg cggcgtcctc gtgaggtggc
     2761 cacggagcgg ctaagcatcg gcgggatcat gcttgagcct gatcgccacc aggtttctgt
     2821 cgacgggaag tccgaactgc tgtcggcgcg cgagtacgcg ctgctcggct acctgatccg
     2881 caacgccggc caggtcgtga cgcgccagca gatcctcgac gcggtctggg gcgccgagcc
     2941 tgacgtgtac tccaacgtcg tcgatctcta cgtccactac ctgcgccgca agctggccgc
     3001 gctcggacgt gaggaagtgc tgcacaccgt gcgtggagtc ggctatgcgc ttcggacgta
     3061 agcgcggaga cggattcgac gcgcccataa ctgccgtcga tcgcgtcgtg cgccgaacgt
     3121 cggtcggcct cgccgtcgtc accctcgtgc tgatcggggc acttctcgcg ggcgtgggcg
     3181 tggtcacggc cgcggtggcc ttgcgagcgt cgagcgatca ggtcgatcgc acgctgaagc
     3241 aggccgctga cagccggata cagcaactcc agcttcaggc cgagcaggtc gagctgccca
     3301 cacccacccc cagcgcctcg cccacctcaa cctcgtcgga cgacgatgaa ccgactgcac
     3361 gcccgactcc cagtccgacc cggacgcgga caccacgccc gacgcaaaca gcgcgccaga
     3421 ccgtacggcc gacgcggacc cgtacccctg aagcgacccg gacgaacgac aacagcgggc
     3481 ctggcaacgg tgatggaacc ccgcgaccgg ctcgcacgga caactccggg tcgcttagtc
     3541 gcgacgcggc catcggcgca ttcgcacagc ttcggctctt ccccacgccc gcaccggcgt
     3601 cgaccacgac ggcgcgtccg acgccatcgg cctcgttcgc gctgccaggc ctgggtgacc
     3661 ggccacccga gtcggccgac actttcttcc tgctgctcaa tacggccggc gacgtcatct
     3721 caaatcccca gggtgtttca ctggatggac tgcccgacga agccgcggtc gtcacggcga
     3781 tcatcaccgg ggaggattgg cgaaccacca ctgtgcgcgg cgtccgcgtc cggctgctga
     3841 ctcagccggt gcgcgacccg gacggcaacc ccgtcggcgt gctgcagtcg ggcttcgtgc
     3901 tcacgctgca ggaccagcag acgaaccagc tgctgaccac gatcgctctc gcgtgcctcg
     3961 tgggcttgac gggcgcggcc ctcatcacgt tgttcatcac ccgccgcgcc atgcggccca
     4021 tccgcagcgc attcgacgcc gagcggcgat tcgtcgcgtc cgcctcccac gagctccgga
     4081 caccggccgc ggtggtgcgc gccaatgccg agatcctgga acgcgaggac ctggtcaagc
     4141 cggagggcaa g
//
LOCUS       DATLCO010000279         4762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5009719, whole genome shotgun sequence.
ACCESSION   DATLCO010000279 DATLCO010000000
VERSION     DATLCO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5009719"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1351)
                     /locus_tag="VMZ33_06440"
     CDS             complement(176..1351)
                     /locus_tag="VMZ33_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HUR16904.1"
                     /translation="MPPITTVEILAVGTELTTGSTRDTNSGDLAKELTDHGVRVLRVA
                     ALPDELEVVATAFRDGIMRADMVISTGGLGPTPDDLTREAIAAACDQEPFVDADLEKW
                     LRGLFERRGIAMPELNRKQAWLIEGAESLPNAHGSAPGWYLARRDGKVIVALPGPPRE
                     MRPMWRDHALPRLEERGLGVSRVVQILRLSGIGESQIAEIIGEDVLRATRPQVATYAR
                     MDAVDVVVSVEDADHALAQRQVDDMVARLRAELGHWVFTQGDGSWPDAIAERLAGRKV
                     AVVELGTAGQLTQMLGDAEFLTFAELLRDPHDLTRAATDLGHYAERVREVGQADIGLA
                     VLARETHGDTHLRVAISTNDESHEEKRVAFLGGSEGRRRAALSACAAFWAWLGERPT"
     gene            1525..3162
                     /gene="groL"
                     /locus_tag="VMZ33_06445"
     CDS             1525..3162
                     /gene="groL"
                     /locus_tag="VMZ33_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HUR16905.1"
                     /translation="MAKLISYDEDARASMKRGMDALAEAVKTTLGPKGRNVALDKKYG
                     APTVTHDGVTVAKDIELDDPFENMGAQLLKEAATKTEDVAGDGTTTATVLAQAIVNEG
                     LKNVASGANPMQMKVGIDKASEAVAEAIRAMAQPVKGRDEIAQVATVSAGDRYIGDLI
                     ADVMDKVGKDGVITVEESKSMEFETEFVEGMQFDRGYVSAYLVTDTQRMEAILEDPFI
                     LITDRKISAIADILPTLEKLVQTGRKDLLIIAEDVDGEALSTLVVNKLRGVISVLAVK
                     APGFGDRRKEMLRDIAILAGGTVISEELGRKLDSVQLEDLGHSRRVVTTKDNTTIVDG
                     AGSADQIAARIKQIKNQIEETTSDYDKEKLQERMAKLSGGVAIIKVGAGTETELKEKK
                     HRVEDALAATRSAVEEGIVPGGGVALLNAQPALDKLSLEGDAAIGVDIVRRALEEPLR
                     QLATNAGLNGQVVLENVRRRGADAQSNTLGYDVIADEYTDLIARGIVDPAKVTRSAVE
                     NAASIGGMVLTTEVMITDKPEPKTAMPQMPPGGMGDF"
     gene            3348..3983
                     /locus_tag="VMZ33_06450"
     CDS             3348..3983
                     /locus_tag="VMZ33_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16906.1"
                     /translation="MQTFDGSGFAEFDRLATLFRRTRELLHEFAATGELPPDAADVIA
                     HEALPHVEDIEQGFRVSLRASAVEIAELRTLIARAGLNTSEPTTSAEARAALIETMHD
                     LALTSTAHAEIVAAPIRYLAALEHARLVFALLPTTAAHEVNFPLGRRTYADIWPPRGA
                     AELADRIEELERNLWTVATQRRPRISDGSYRRTYGFFDTAARMSGHGFQAA"
     gene            3989..>4762
                     /locus_tag="VMZ33_06455"
     CDS             3989..>4762
                     /locus_tag="VMZ33_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16907.1"
                     /translation="MNTPSEQPQAEPRIDSARRRPASLLGQFGETRRSFMGLINAHVG
                     LFKAEINEILGLVKIIGALAAVVLTVALMTSTLLYVGGFLFMGEWLFGSMGWGLAHGF
                     LFGVGLIVALVLAILGARGGRIALAFVLGTVVAVAIALLCGSNVAYDAASSVAANLAS
                     PLNSAGVVALLAGAVIGAIAFALMLWRLAGAGGAIGGLVIGALLGALVGWLIAGAPWT
                     WPPAVGFAITIGLLAWIIFQVVLAVPTLDIEKRFSRLYPR"
BASE COUNT          812 a         1584 c         1567 g          799 t
ORIGIN      
        1 gacccacacg cacggcagcc aattcccggt ggctcattgg cacgcgcgtc gcccatgccg
       61 atcagctcgt cgtacgggcg tgcacccgtc acgtcgatcg ctgggaagtt gaccagctcc
      121 agctcatgcg cgccgcacac cggacacgct tccttcgagc gctgctcttc gttcgtcatg
      181 tcggccgctc cccgagccac gcccagaagg cggcgcacgc actgagcgca gcgcgtcgcc
      241 ggccctcgct gccacccagg aacgcgacgc gcttctcctc gtgtgattcg tcattggtcg
      301 agatcgcgac gcgcaggtgc gtgtcgccgt gtgtctcccg cgccaggacg gcaaggccga
      361 tgtcggcttg tcctacctcg cgcacgcgtt cggcgtagtg gcccaggtcg gtggctgcgc
      421 gggtcaggtc gtgcgggtcg cggagcagct cggcgaaggt caggaactcg gcgtcgccga
      481 gcatctgtgt gagctgaccg gccgtgccca gctcgaccac ggcgaccttt cggcccgcaa
      541 ggcgttcggc gatcgcatcc ggccacgatc cgtcgccctg cgtgaagacc cagtggccga
      601 gctcggcccg cagccgggcg accatgtcgt cgacttggcg ctgcgcgagc gcgtggtcgg
      661 cgtcctccac cgagacgaca acgtccacgg catccatccg ggcataggtg gcgacctgtg
      721 gccgcgtagc gcgcagcacg tcctcgccga tgatctcggc gatctggctc tcaccaatgc
      781 ctgagagccg caggatctgg accacccggc tgacgcccag cccgcgctcc tccaggcgtg
      841 ggagggcgtg gtcgcgccac atcggccgca tctcgcgcgg gggaccgggt aatgcgacga
      901 tcactttgcc gtcgcgccgc gccaggtacc agcccggcgc cgatccatgc gcattcggga
      961 gcgactcggc tccttcgata agccaggcct gcttgcggtt cagctccggc atggcgatgc
     1021 cgcgccgttc gaagaggcca cgcagccact tctccaaatc cgcgtcgacg aagggttcct
     1081 gatcgcacgc ggcggcgatc gcctcgcggg tcaggtcgtc gggcgtcgga cctaggccac
     1141 cagtggagat caccatgtcg gcgcgcatga tgccgtccct gaacgcggtg gcgaccactt
     1201 ccagctcgtc ggggagtgcc gcaacacgca gcactcgaac cccgtgatcg gtcagctcct
     1261 tcgccaggtc tccggagttc gtgtcgcggg tggatcctgt cgtgagctcg gtgcccacgg
     1321 cgaggatctc gacggtggta atcgggggca tcgaattcac cgtagcagcg gatgcgctgc
     1381 aggcgtgcga acgttgacgg agcgcgccaa atgtgttagc aatctcctac cgagagtgct
     1441 aacgaacggt ggcgtcgcaa agccccgctg acgcagccgt caggcgccgt aaagacatca
     1501 cacccgagag gtagccggac agacatggcc aaactcattt cgtacgacga ggacgcacgc
     1561 gcgtcgatga agcgcggtat ggacgcgctt gccgaagccg taaagacaac gctcggcccc
     1621 aagggccgga acgttgccct ggacaagaag tacggcgccc ccacggtcac gcacgacggc
     1681 gtcaccgtgg ccaaggacat cgagctggac gacccgttcg agaacatggg cgcgcagctt
     1741 ctcaaggaag ccgcgaccaa gaccgaggat gtcgctggtg atggcaccac cacagccacg
     1801 gttctcgccc aggcgatcgt caacgagggc cttaagaacg tcgccagtgg cgccaacccg
     1861 atgcagatga aggtcggcat cgacaaggcg tctgaggctg tggccgaggc catccgggcg
     1921 atggctcagc cggtcaaggg ccgcgacgag atcgcccagg tggcgaccgt gagcgccggc
     1981 gatcgataca tcggtgacct catcgccgac gtcatggaca aggtcggcaa ggacggcgtc
     2041 atcaccgtcg aagagtcgaa gtccatggaa ttcgagacgg agttcgtcga gggcatgcag
     2101 ttcgaccgcg ggtacgtgag cgcgtatctc gtgaccgaca cgcagcgcat ggaggcgatc
     2161 ctcgaggatc ccttcatcct catcaccgat cgcaagatca gcgccatcgc ggacatcctg
     2221 ccgaccctcg agaagctggt ccagacaggc cgcaaggatc tgttgatcat cgccgaggat
     2281 gtagatggcg aggctctgtc gacgctcgtc gtcaacaagc ttcgtggcgt gatcagcgtt
     2341 cttgccgtga aggccccggg cttcggcgac cgtcgcaagg aaatgctgcg cgacatcgcc
     2401 atcctggccg gtggcacggt catcagcgag gagctgggcc gcaagcttga ctccgttcag
     2461 ctggaggatc ttggccactc ccgccgcgtc gtgacgacca aggacaacac cacgatcgtg
     2521 gacggcgcgg gttcggccga tcagattgct gcgcgcatca agcagatcaa gaaccagatc
     2581 gaggagacca cctccgacta cgacaaggag aagctccagg agcggatggc caagctgtcc
     2641 ggcggtgtcg cgatcatcaa ggtcggcgcc ggcacggaga ctgagctcaa ggagaagaag
     2701 caccgcgttg aggacgcact cgccgccacc cgctcagcgg tggaggaggg gatcgtcccg
     2761 ggcggtggcg tcgcgcttct gaatgcccag ccggcactcg acaagctgtc gcttgaaggt
     2821 gacgcggcta tcggcgtcga catcgttcgt cgcgcgttgg aggagccgct tcggcagctc
     2881 gccacgaacg ccggtctcaa cggccaggtt gtcctcgaga acgtccgacg ccgtggcgcg
     2941 gatgcacaga gcaacaccct cggctacgac gtgattgcgg acgagtacac cgacctcatc
     3001 gcgcgtggca tcgtcgatcc ggcgaaggtc acccgttcgg cggtggagaa tgccgcgtcg
     3061 atcggtggca tggtgctcac gacggaggtc atgatcaccg acaagcctga gcccaagacg
     3121 gcgatgccgc agatgccgcc gggcggcatg ggcgacttct aggcactccc cgaggcccaa
     3181 cacgagaagc ccggcccgtt ggccgggctt cttcgattcc gtcgtactta tcggccccgg
     3241 ttgtcgccga cccgttcgac ttcgcgttgt cgcagcgaag ctgaaaggga cgcccgtgcg
     3301 cggcgctgtt acgcggcaag ggtcatcccg cttagcattc atcacagatg caaacgttcg
     3361 acgggtccgg gttcgccgag ttcgataggt tggcgacgct gttcaggcgc acgcgcgagc
     3421 ttcttcacga gtttgccgca accggcgagc tgccccctga cgcggcggac gtcatcgctc
     3481 acgaggctct gcctcatgtc gaggacatcg agcagggctt ccgggtgtca ctccgggcga
     3541 gcgccgtcga gatcgccgag ctgcgaaccc tgattgcacg cgccggactc aacacgtccg
     3601 agccaacaac gagcgctgag gcgcgcgccg cgctgataga gacgatgcat gacctggcgc
     3661 tgacgagcac ggcacacgcc gagatcgtcg ccgccccaat tcggtacctg gccgcacttg
     3721 agcatgcgcg gctggtcttc gccttgctcc cgacgacggc cgcgcacgag gtcaatttcc
     3781 cgctcggtcg ccggacctat gccgatatct ggccgccacg tggcgccgcg gagctggcag
     3841 accgcatcga ggagctcgaa cgcaacctat ggaccgtggc cacgcagcgc cgcccgcgca
     3901 tcagtgatgg cagctatcgg cgcacctacg ggttctttga cacagccgcc cgcatgagcg
     3961 gccacggctt tcaggcggcc tgatcacgat gaatacccca agcgagcagc cccaggcgga
     4021 accgcgcatc gatagcgccc ggcggcggcc cgcatcgctg ctcgggcagt tcggcgagac
     4081 gcgcaggtcg ttcatggggc tcatcaacgc tcacgttggg ctcttcaagg ctgagatcaa
     4141 cgagatcctc ggcctcgtga aaatcatcgg tgccctggcc gcggtcgtcc tcacggtggc
     4201 cctgatgacc agcacattgc tgtatgtcgg tggcttcctg ttcatgggcg aatggctctt
     4261 cgggtccatg ggttggggtc tcgcccacgg cttcctgttc ggcgtcggcc tcatcgtcgc
     4321 cctggtgctc gcaattctcg gtgcaagggg tggccgcatc gcccttgcgt tcgtgctcgg
     4381 caccgtggtc gcagtcgcga tcgccctctt gtgcggatcc aacgtcgcct acgacgccgc
     4441 ctcgagcgtt gccgcaaacc tcgcctcgcc gctgaacagc gccggggtcg tcgccttgct
     4501 ggccggggcc gtaatcggag cgatcgcgtt cgcactgatg ctgtggcgcc ttgcgggcgc
     4561 cggtggcgcc atcggtgggc tggtgatcgg agccctcctc ggcgcccttg tgggctggct
     4621 gatcgccgga gcgccgtgga cgtggccgcc cgccgtcggg ttcgcgatca cgatcggcct
     4681 gctcgcgtgg atcatcttcc aggtggtgct ggcagtgccc actcttgaca ttgagaagcg
     4741 tttctcgcgg ctctatccgc ga
//
LOCUS       DATLCO010000280         2525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6985496, whole genome shotgun sequence.
ACCESSION   DATLCO010000280 DATLCO010000000
VERSION     DATLCO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6985496"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VMZ33_06460"
     CDS             <1..1021
                     /locus_tag="VMZ33_06460"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HUR16908.1"
                     /translation="AELGDDVFGDDPTVNALEERAAELTGKQAALFVASGTMGNLVCL
                     MTHVPRGGEIIAGDGAHSFAHEAGNWAVVVGASMKLLPWDESGRMDLDHIRRAFRNPN
                     DVHDPITSVIALENTHADSMAQPLPEDYTADVAKIAKAGGVPVHVDGARIFNASIALQ
                     VPVRKLLAHADSASFCLSKGLACPVGSVVVGDRDFIWKARRARKLLGGGMRQAGVLAA
                     AGLIALQDGPEGMIERLAEDHDNARRLADGLADLPGIVKLEPARVRTNFVFFELAQPD
                     LRLKFLDALKDEGALMIDYPGGDRIRAVTHYGIEPADIDATIAATHRALIKVGIAGKS
                     QPVAA"
     gene            1018..>2525
                     /locus_tag="VMZ33_06465"
     CDS             1018..>2525
                     /locus_tag="VMZ33_06465"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUR16909.1"
                     /translation="MTVTDSESITDQNAPAPQTESDRRFSALVRDRFDAIMRRYPVFA
                     TYLGLHQLDGEVGDGSRDAILADLEQARAFEAALETISPAELSPYYAFERELALFATR
                     RQIFDDDVHRVWERRVNAADEIGDGVFLIFARGTRPLAERLVDICSRLEQAPRHIEEQ
                     KTRLGGPRPPVRLWNEMELEAASSMPSLFDEVIAAARTELGADHADVMRLEQAATVAR
                     EALLSYAAWIRERLTDATDDWALGRADYDRLIDLRAFDGLDSDEILAIGEEQLALNRE
                     ARRKLAVEIDPSATEHEVLDRVKSDHPADFVTALKTYRSAMAEARHFVVEHDIVTMPE
                     GESLSVIETPEYLRNVMPFAAYFSPPKFAAQDRHGIYIVTPSVDGDPRAMREHNFASI
                     YNTSIHEAYPGHHQQLAAAIGHPSLVRLLIDAPEFVEGWAMYCEQMMREEGFDTQPEH
                     RLMMYTDAIWRACRIILDIRLHRGELTVEEATDFLVEQTGFERPQAAAEVNR"
BASE COUNT          435 a          865 c          828 g          397 t
ORIGIN      
        1 ggccgagctc ggcgacgacg tgttcggcga cgacccgacc gtcaacgcgc tggaggagcg
       61 cgccgccgag ctgaccggca agcaggccgc gctcttcgtc gccagcggaa cgatgggcaa
      121 cctcgtttgc ctcatgactc acgtgccacg cggcggcgag atcatcgcgg gcgacggggc
      181 gcattcgttc gcccacgagg ccggcaactg ggcagtcgta gtcggcgcgt ccatgaagct
      241 gctcccgtgg gacgaatcgg gccggatgga cctcgaccac atccgccgcg cgttcaggaa
      301 tccgaacgac gttcatgacc cgattacgtc ggtcatcgcc ctcgagaaca cccatgccga
      361 ctccatggcg cagccactcc ccgaggacta caccgcggac gtggccaaga tcgcgaaggc
      421 aggcggcgtc cccgtccacg tcgacggagc gcgcatcttc aatgcctcga tcgccttaca
      481 agtgccggta cgcaagctgc ttgcccacgc cgattcggcg agcttctgcc tgtcgaaggg
      541 cctcgcgtgt ccagtcggct cggtggtcgt cggcgatcgc gacttcatct ggaaggcgcg
      601 ccgagcgcga aagctcctcg gtggcggcat gcgtcaggcc ggcgtcctcg ccgcggccgg
      661 cctcatcgcg ctgcaggacg gtcccgaagg aatgatcgaa cgcctcgctg aagaccatga
      721 caacgctcgc cggctggccg atggcctggc cgacctcccc gggatcgtga agcttgaacc
      781 ggcccgtgtt cggaccaact tcgtcttctt cgagctcgct cagcccgacc tgcgcctgaa
      841 gttcctcgac gcgctcaagg acgagggcgc gctgatgatc gactaccccg gcggtgaccg
      901 gatcagggca gtcactcact acggcatcga gccggcggac attgacgcga cgatcgcggc
      961 cacgcaccga gcgctcatca aggtcggcat cgcgggcaag agccagccag tcgcggcgtg
     1021 acggtcaccg actcggagtc gattaccgac cagaacgctc cagctcccca gacagagtcg
     1081 gaccgtcgtt tcagcgcgct cgtccgggac cgcttcgacg cgattatgcg ccgctacccc
     1141 gtctttgcaa cgtacctagg cctgcaccag ctcgacggtg aggtcggcga cggcagccgc
     1201 gacgcgatcc tggcggacct cgagcaggcg cgcgcgtttg aggcggcgct cgagacaatc
     1261 agccccgccg agctgagccc gtactacgcc ttcgagcgcg agctggcgct cttcgccaca
     1321 cggcgccaga tctttgacga cgacgtgcat cgcgtctggg agcgccgggt caacgccgcg
     1381 gacgagatcg gcgacggcgt gtttctcatc ttcgcccgcg ggacgcggcc gttggccgag
     1441 cgcctcgtgg acatctgttc gcgcctcgag caggcgccgc ggcacatcga ggagcagaag
     1501 acccggcttg gcggtcccag gccgcccgta cgtctctgga acgagatgga gcttgaagct
     1561 gcgtcttcga tgccctcgtt gttcgacgag gtcatcgccg cggcgcgcac ggagctgggc
     1621 gcggaccacg ccgacgtcat gaggctcgag caggcggcaa ccgttgctcg cgaggcgctc
     1681 ctgagctacg cggcctggat tcgcgagcgt ctgaccgatg ccaccgacga ctgggcgctc
     1741 ggccgcgcgg actacgaccg gttgattgac ctgcgtgctt tcgacgggct cgacagcgac
     1801 gagatcctcg ccatcggaga ggagcagctg gcgctcaacc gcgaggcacg tcggaagctc
     1861 gcggtggaga tcgaccccag cgcgacggag cacgaggtgt tggatcgggt caagtccgac
     1921 catccggccg acttcgtcac ggccctcaag acctatcgca gcgccatggc tgaggcccgc
     1981 cacttcgtag tggagcacga catcgtcacg atgccggaag gcgaatcgct gtctgttatc
     2041 gagacgcccg agtacctgcg caacgtgatg ccgttcgcgg cgtacttcag cccgcccaag
     2101 ttcgcggcgc aggaccggca cggcatctat atcgtcacgc cctcggtcga tggcgacccg
     2161 cgggccatgc gcgagcacaa cttcgcctcg atctacaaca cgagcattca cgaggcctat
     2221 cccggccatc accagcagct ggcggccgca atcggacacc cgagcctggt gcggctgctc
     2281 atcgacgccc cggagttcgt cgagggctgg gccatgtact gcgagcagat gatgcgcgaa
     2341 gaagggttcg acacccagcc tgagcaccgg ttgatgatgt acacggacgc catctggcgg
     2401 gcgtgccgga tcatcctcga cattcgcctg catcgcggcg agctcaccgt ggaggaggcg
     2461 accgacttcc tggttgagca gacgggcttc gagcgaccgc aggctgcggc cgaggtcaat
     2521 cgcta
//
LOCUS       DATLCO010000281         5763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4254936, whole genome shotgun sequence.
ACCESSION   DATLCO010000281 DATLCO010000000
VERSION     DATLCO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4254936"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VMZ33_06470"
     CDS             complement(<1..495)
                     /locus_tag="VMZ33_06470"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HUR16910.1"
                     /translation="MTSLESGARSGPDGELEPLDDPLLRDSALDLATLAAHWAAYARR
                     QPMTAENMTGADRRAQRMGRTSRGLMEQAGFAVAVTARALLNSVDRVSSGRILVLCGP
                     GNNGGDGFVAARHLAEFGLSSYAVLVSGAEQPGAPDARANWDALAEMPLVERIHVGSA
                     QDALL"
     gene            559..1401
                     /locus_tag="VMZ33_06475"
     CDS             559..1401
                     /locus_tag="VMZ33_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HUR16911.1"
                     /translation="MRSIWRGAINFGMVTIPCKLYTATEQKDVRFRLVHKKDGAPIVE
                     KRFCSEEDKEVPWDEVGKGYEVSKGDFVVMDQEEIDEAAPERTHTIDIGDFVELDEID
                     PIYFEKHYFLEPVDVGAKPFNLLRRALEETGRVAVAKVAIRTKERLATLRGYQDTMIL
                     ETMYWPDEIRSIAELDLPSGKAAAPATKEIDMARTLIENLAGHFDPKQYTNEFRSTLM
                     ELIERKMKGEQRSAKRRKADPKVVDLMEALQASLAATKARSTGGKARGSTPRPKSGAR
                     KKAA"
     gene            complement(1398..2060)
                     /locus_tag="VMZ33_06480"
     CDS             complement(1398..2060)
                     /locus_tag="VMZ33_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16912.1"
                     /translation="MALIAYGLIGIAIFVFVAQAINRPLERVRELSESVEDQRAALVE
                     SMDQAEQTIRQMAAAVGNMDTSLSDAKIATERSSTISGGLATSMFLLRDQMFVTIPII
                     EAQPFVGLAPGFDQTGQQLQQLSIDLSTIGTSLDTNRGDVVATSENLVDLARSVGDLT
                     EALQAGPPVAITEDAVDSFRLAIFAIAGWVVLFAVACVLVGLYLIFSGRRAVREMEDD
                     LA"
     gene            complement(2131..3336)
                     /locus_tag="VMZ33_06485"
     CDS             complement(2131..3336)
                     /locus_tag="VMZ33_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16913.1"
                     /translation="MRDLVVGPFKSLIRITSQISKELIQVRRRPGAFFSLVLGPFAIM
                     AIFGLGYTGVHRPLDTVIVVPSDLAIQRDAAFYQNIVGGALTIKEVTTDSAAAMEELA
                     AQRLDLVVVAPTDAAGQFRAGKQANLDVYLNSVDPVAVGYSDFLASVVNQRVNEEIIR
                     QAVTQGQNYAVAGTAGQFEEIPPEVVARPTTIATHNLAQSTPSVVSYAAPAAFALMLQ
                     HMAVTLTALSFVRERLGGAMELFRISPINSLELVLGKYLGLGLVSAIIAVITTVLLVT
                     VLGVPLLGNVWLVAAIIALLVFASLGLGLLISVIADSERQAVQLALLLLLASVFFSGF
                     VLPINEFREGVQYGAYALPVTHGIRLLQDIMLRGDTTAWWQVSVLAAIGVVLLILTTL
                     ALRRSMRNA"
     gene            complement(3333..4379)
                     /locus_tag="VMZ33_06490"
     CDS             complement(3333..4379)
                     /locus_tag="VMZ33_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR16914.1"
                     /translation="MPELTPTTPQMGTDAPAATRAIWGPDRRLAVQITDASKSFGAVM
                     GVAGISLSVPQSAIVGIIGPSGAGKTTTIRLITGSLAPDRGEVRVLGEDPRHFRRRTR
                     EQIGYMPQGFVLYPDLTARENVSFMASLFGMAPWRRGRRVNDTLKLVELWDARDRRAS
                     ALSGGMQRRLELACALVHAPSLLILDEPTAGIDPLLRTRIWQELERLRQAGVTVLVTT
                     QYVSEAEYCDAVALISEGDLVAFSTPQDMRKQALGGDVIEVTTSEPIDARSLPPEEGV
                     ISVRQTAPRQLLFIVADAGTATPRVNDMIERAGGTVEYSREYRPTFDEVFSALVTAHA
                     GRNSDGATLQGDLA"
     gene            4656..>5763
                     /locus_tag="VMZ33_06495"
     CDS             4656..>5763
                     /locus_tag="VMZ33_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16915.1"
                     /translation="MSAMSSNEMYLAARAAGLRYVDRTDPGIRRVKRGDAFGFLDLAG
                     KAVRDAATLSRIKALVIPPAWTEVWICSSANGHIQATGRDARGRLQYRYHADWARVRD
                     ETKYERTLGFARALPRLRRRVTRDLALRGLPRDKVLATIVRLLEMTMVRVGNEEYARE
                     NKSFGLTTLRDRHVKVRGAELKLSFRGKSGKDHAVGIEDRRVARIVKACQDLPGQRLF
                     QYLDEDGNRQSVDSDDVNQYMRAAMGDEFSAKDFRTWAGTVLAARAFQALEAATGDEQ
                     LAEAAAAGVTNRGRSSLTRAVEEVAQQLGNTPAVCRKCYIHPQIIDAYMDGSLAEALT
                     RRAGEVLETDKGLRAEERFVLSILQRRLTADNRKA"
BASE COUNT         1074 a         1820 c         1960 g          909 t
ORIGIN      
        1 cagcagcgcg tcctgcgccg atcccacgtg gatccgctcc accagcggca tttccgcgag
       61 tgcgtcccag ttggccctgg catcgggcgc tcccggctgc tccgcgccgc tcaccagcac
      121 cgcatacgac gacaggccaa attcggccag gtgccgcgct gcgacgaacc cgtcgccgcc
      181 gttgttgccc ggcccgcaga gcacgaggat gcgccccgag gacacacgat cgacggagtt
      241 gaggagtgcc cgggccgtca cagctacagc gaaacctgct tgctccatga ggccacggct
      301 ggtccggccc atccgttgcg cacggcggtc cgccccggtc atgttttcgg cggtcattgg
      361 ctgacgtcgc gcgtaggcgg cccagtgagc cgcgagcgtg gccaggtcca gcgccgagtc
      421 gcgcaggagc ggatcgtcga gcggctcaag ctcgccgtca ggcccggacc ttgcacccga
      481 ttcaagggaa gtcatctgag agatcgtcgc accccgcggg tatggaggtc aggagtggcg
      541 cgtacctagg ctcgttacat gagatcgatc tggcgtggcg ccatcaattt cgggatggtc
      601 accatcccct gcaagctcta caccgcaact gagcagaagg acgttcgctt ccggctcgtc
      661 cacaagaagg acggcgcgcc gatcgtggag aagcgcttct gctccgagga ggacaaggag
      721 gtcccctggg acgaggtcgg gaagggctac gaggtcagca agggcgactt cgtcgtgatg
      781 gaccaggagg agatcgacga ggccgcgcct gagcggacgc acaccattga tatcggcgac
      841 ttcgtcgagc tcgacgagat cgacccgatc tacttcgaga agcactactt cctcgagccc
      901 gtcgacgtcg gcgccaagcc gttcaacctg ctgcggcgcg ccctcgagga gacgggccgc
      961 gttgccgtgg ccaaggttgc gattcgaacc aaggaacgcc tcgcgacgct acggggctac
     1021 caggacacca tgatcctcga gacgatgtac tggccggacg agatccgctc cattgcagag
     1081 cttgaccttc cgtcaggcaa ggccgcggca cccgcgacaa aggaaatcga catggcccgc
     1141 acgttgatcg agaacctggc cgggcatttc gacccgaagc agtacaccaa cgagtttcga
     1201 tcgacgctga tggagctgat cgagcggaaa atgaagggcg agcagcggtc ggccaagcgg
     1261 cgcaaggcgg accccaaggt cgttgacctc atggaggcgc tgcaggccag tctggctgcg
     1321 acgaaagctc ggtcgactgg gggcaaggca agggggtcca cgccccgacc gaaatcgggc
     1381 gctcgcaaga aggcggccta ggccaggtcg tcctccatct cgcgcaccgc gcgtcggccg
     1441 gagaagatca ggtacaggcc gacgagcacg caggcgaccg cgaataggac cacccaccct
     1501 gcgatggcga atattgccaa ccggaacgag tcgacggcgt cctcggtaat cgccactggc
     1561 ggcccggcct ggagtgcctc cgtgaggtcg ccgactgacc tggcgaggtc gaccagattt
     1621 tcggaggtgg caacgacgtc cccgcggttg gtatcgagcg aggtgccgat cgtggacagg
     1681 tcaatggaaa gctgctggag ctgttggccg gtctggtcga agcccggagc gagcccgacg
     1741 aacggctgtg cctcgatgat cggaatggtg acgaacatct ggtcgcggag tagaaacatg
     1801 ctcgtggcca acccgcccga gatggtcgac gaccgctcgg tcgcaatctt tgcgtccgac
     1861 aggctcgtgt ccatgttgcc cacggccgcc gccatctggc ggatggtctg ctcagcctga
     1921 tccatcgact ccacgagcgc tgcgcgctgg tcctcgaccg attccgatag ctcacgcacg
     1981 cgctcaagcg gacggtttat ggcctgcgcc acgaacacga agatcgcgat gccgatgagt
     2041 ccgtatgcga tcaatgccac gcccgttagc tgcgaacggc gaccgcgcct gcgtgcgcga
     2101 cgaatcaggt agtggcggcg atccgctgcg tcaggcattc cgcattgatc ggcggagggc
     2161 gagcgtcgtc aggatcagca gaacgacgcc gatcgcggca agcacgctga cctgccacca
     2221 ggcagtcgtg tcgccgcgca acatgatgtc ctgcaagagc cgaatgccgt gcgtcaccgg
     2281 cagagcgtac gccccgtatt ggactccctc gcggaactcg ttgatgggca gaacgaaacc
     2341 tgagaagaac accgacgcga gcagcagtaa gagggcaagc tgaacagcct ggcgctccga
     2401 gtcagcgatg accgaaatga gcaggccaag gccaaggctg gcgaacacga gcagggcgat
     2461 gatggcggcc acgagccaca cgttgcccag cagtggaacg cccagcaccg tcacgagaag
     2521 cacggtcgtg atgacggcga tgatcgcgct gacaagcccc aagccgaggt atttgccgag
     2581 cacgagctcg agcgagttga tcggtgagat cctgaagagc tccatggcgc cgccgagccg
     2641 ctcgcggacg aacgacaacg cggtgagcgt cactgccatg tgctggagca tcagtgcgaa
     2701 cgctgcgggg gccgcgtacg acacgacgct cggcgtcgac tgcgcgaggt tgtgcgttgc
     2761 gatcgtggtc ggtcgagcta cgacctccgg aggaatctcc tcaaactgcc cggcggtgcc
     2821 ggcgacggca tagttctgac cctgggtgac cgcctgccga atgatctcct cgttgacgcg
     2881 ctggttaacg accgaggcca ggaagtcgga gtagccgacc gcgacggggt cgactgagtt
     2941 caggtagacg tcgaggttgg cctgcttccc agcacggaat tggcccgcgg cgtcggtcgg
     3001 ggcgaccact accaaatcga gacgctgggc agcgagctcc tccatcgcgg cagcagagtc
     3061 ggtcgtgact tccttgatgg tgagcgcccc accgacgatg ttctggtaga aggccgcatc
     3121 ccgctgaatg gccaggtcgg atggcacgac gatgaccgtg tcgagcggcc tgtgaacgcc
     3181 ggtgtagccg agaccgaaga tggccatgat cgcgaaaggg ccgaggacga ggctgaagaa
     3241 ggcgcccggc cggcgccgaa cctggatcag ctccttgctg atctgggaag tgatgcggat
     3301 taatgatttg aaggggccga caaccaggtc tctcacgcga gatcgccctg gagcgttgcg
     3361 ccatcagaat tgcgtcccgc gtgcgccgtg actagcgccg agaacacctc gtcgaaagtc
     3421 ggccggtact cgcggctgta ttcaacggtg ccgcccgccc gttcgatcat gtcgttcacc
     3481 cggggagtgg cggtccctgc atcagccacg atgaagagca gctggcgagg tgcggtttgc
     3541 ctgacggaga tgacgccctc ctccggtggc agggagcgcg cgtcaatcgg ttcactggtc
     3601 gtgacttcaa tgacgtcacc gccgagcgcc tgcttgcgca tgtcctgcgg cgtggagaac
     3661 gcgacgaggt cgccctcaga tatgagcgcg acggcatcgc agtactcggc ctcgctgacg
     3721 tactgggtcg ttacgagcac agtcactccc gcctggcgca gccgttcgag ctcctgccag
     3781 atgcgagtgc gcaggagagg gtcgatgccc gctgtcggtt cgtcgaggat gagcaggctg
     3841 ggtgcatgca cgagggcgca ggcgagctcg aggcgccgct gcattccccc cgaaagggct
     3901 gatgcacggc gatcccgcgc gtcccacagc tccacaagct tcaaggtgtc gttgacgcgg
     3961 cggccgcgcc gccacggcgc catgccgaag agcgaggcca tgaagctgac gttctcgcgc
     4021 gccgtcaggt ccgggtagag cacgaagccc tggggcatgt agccgatctg ctcgcgcgtc
     4081 cgtcgacgga aatggcgcgg gtcctcaccc agcacacgaa cctctccccg gtccggcgcc
     4141 agcgaaccgg tgatgaggcg aatcgtcgtt gtcttgccgg cgccgctggg gccgatgatg
     4201 ccgacgatcg cgctctgcgg cacggacagg ctgataccgg caacgcccat gaccgcgccg
     4261 aagctcttcg aggcgtcggt gatctgcacg gcgagccggc ggtcgggccc ccagatcgcg
     4321 cgtgtcgcgg ccggagcatc cgtccccatc tggggcgtgg tcggggtcag ttcgggcacc
     4381 acatcgggcg gcccacccag ggactcagtg ccgagcggca gcgtgttcgg gacaggcgca
     4441 gtcggtgtgt tcgggacagg tgcagatggc tggtgagacg ggtatgacga cacagcctgc
     4501 gcatcttttg cgcttcgcgc ttgcaccgac agacggcgag ccgccccacg gcgatgcaaa
     4561 ggcatcccaa ccgtgccgct gttagcgggc tgcaacggcg ttaactcggt ctggtcgtga
     4621 cggataccgg ccgcgctggc tagggtcgaa acgacatgag cgccatgagt tccaacgaga
     4681 tgtacctggc ggcgcgtgcc gccggattgc gttacgtcga ccgcacagat ccagggatcc
     4741 gtcgcgtcaa acggggcgac gcttttggct ttctggacct tgccggcaag gcggtgcgcg
     4801 atgcggccac gctgtcacgc atcaaagccc tggtgatccc cccggcgtgg accgaggtat
     4861 ggatctgctc gtcggccaac ggccacatcc aggcaactgg ccgcgatgcc cgtgggcggc
     4921 tgcagtaccg ctatcacgcc gactgggcca gggtccgcga cgagacgaag tacgagcgga
     4981 cgctcgggtt tgcacgggcc ttgccccggc tgcgacgccg cgtgacgcgc gacctggcgc
     5041 tgcgtggatt gccgcgcgac aaggtcctgg cgacgattgt gcgtctgctc gagatgacca
     5101 tggtccgcgt cggcaatgag gaatatgcgc gcgaaaacaa gtcgttcggc ctgaccactc
     5161 tgcgtgatcg gcacgtcaag gttcgtggcg cggagctgaa gctctcgttc cgcgggaaat
     5221 ccggcaagga ccatgcagtg ggaatcgagg accggcgcgt ggctcgcatc gtcaaggcat
     5281 gtcaagacct gccgggccag cggctcttcc agtacctgga tgaggacggc aaccgccaga
     5341 gcgtggactc cgacgacgtg aatcagtaca tgcgcgccgc gatgggcgac gagttcagcg
     5401 ccaaggattt ccggacctgg gctggcacgg tgctggcagc tcgcgcgttt caggcgctcg
     5461 aggcggcaac cggtgacgag cagctcgccg aggcggccgc ggccggggtc acgaaccgcg
     5521 ggcgctcttc gctcacacga gctgtcgaag aggtggcaca acagctgggc aacacgccgg
     5581 cggtgtgtcg caagtgctat atccacccgc agatcatcga tgcgtacatg gatggctcgt
     5641 tggccgaggc gctcacccgt cgcgccggcg aagtgctcga gaccgacaag gggctccggg
     5701 cggaggaacg cttcgtgctg tcgatcctcc agcgacgcct caccgccgac aaccgaaagg
     5761 cag
//
LOCUS       DATLCO010000282         3127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10716893, whole genome shotgun sequence.
ACCESSION   DATLCO010000282 DATLCO010000000
VERSION     DATLCO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10716893"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VMZ33_06500"
     CDS             complement(<1..421)
                     /locus_tag="VMZ33_06500"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HUR16916.1"
                     /translation="MTGEILKVAAANAAVIFVLALAMWSIAVRINDPSFIDTCWGAGF
                     VVVAWVSFLMTDASSSRRPVLLAIATVWGLRLAGYLFWRWRRNGPDARYMAMLKHAPN
                     PKMYMLTKVFLLQSVLLLVVSTPLQLGQAYSGGFNAGT"
     gene            complement(468..1385)
                     /locus_tag="VMZ33_06505"
     CDS             complement(468..1385)
                     /locus_tag="VMZ33_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUR16917.1"
                     /translation="MAPSLMLFILMATAGSVLLAAGFYERLFAGNSASQYLASLEDHD
                     DIEDDFVRKLHEPLIARTLGSLRGVTSPLLRRVTPAGLLDKARRDLMLAGMKVAPEEL
                     LTLQVALGALGLVAAIAFASMGGKPGLTLLVLILMPGAGLLIPSAIVKRKGAERAESI
                     FKDLPDCLDLLAISVEAGVGFEAALDVVCNHFDSALAEEFRLTLREMELGLSRREAMQ
                     NLRARTDVPELSNFILALTQADALGMPIGRVLHTQATELRNRRRQWAREKAAKLPVKI
                     MFPLVLFIFPSILVVVLFPAAGGIAKALA"
     gene            complement(1388..2374)
                     /locus_tag="VMZ33_06510"
     CDS             complement(1388..2374)
                     /locus_tag="VMZ33_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUR16918.1"
                     /translation="MMRVLIPIIVGLGLSTIVVGFLLRARERAAALNSLMDLARFGED
                     AFGEEEEAQIEAAKSVVDVSHITRGAVSLAGRLIAQVDEQGTLAASLERARIPLRAGE
                     YVVVAACVSTVGALLIATITSSPILGGMVFALAVFGTAKFPKLRQARRRKALSRQLPD
                     ALSLIASSLSAGHTFLRSIQMMCEEADPPLADEFGRVVFEVRLGAPLVDALSNMSDRV
                     GVADLAWVVQAIRIQQTVGGKLADLLHTLADFIRARDEVRREVDVLTAEGRISAWVLG
                     GMPIFLLLALQTLNPGYAKPLFHGAGLFALAFTAVSICVGVGIIFKMVKIEV"
     gene            complement(2371..>3127)
                     /locus_tag="VMZ33_06515"
     CDS             complement(2371..>3127)
                     /locus_tag="VMZ33_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HUR16919.1"
                     /translation="VSGGTGTGKTTTLNVLSSFIPEEERIITIEDSAELQLQKPHVVS
                     LEARPSNAEGVGEVRIRDLVKNALRMRPDRIIVGECRGAEALDMLQAMNTGHEGSMTT
                     VHANNPRDALSRLETMVLMAGFDLPVRAIREQVNAAIDVIIQLERFPDGRRVVTAVTE
                     VQGMEGDTILLQDVFKLRHGPNGTTGDVVPTGLRPRFVERIAERGVELPADVFRVTRA
                     MSGAPRVARATRLNGPSLPGHDGYIEPKVEANQ"
BASE COUNT          597 a          975 c         1062 g          493 t
ORIGIN      
        1 cggtgcctgc gttgaacccg ccgctgtatg cctgccccag ctgcaacggc gtcgagacaa
       61 ccagcagcag caccgactgc aagaggaaca ccttggtcag catgtacatc ttcgggttcg
      121 gcgcgtgctt cagcatggcc atgtaacggg cgtcagggcc gttacgacgc caccgccaga
      181 acaaatagcc cgccagccgc agcccccaca ccgtggcgat ggcgagcagg acaggccggc
      241 gtgaggatga ggcgtccgtc atgaggaacg agacccatgc gacgacgacg aaccccgcgc
      301 cccaacaggt gtcgatgaac gacgggtcgt tgatgcgcac cgctatcgac cacatcgcga
      361 gcgccaaaac gaaaatgacc gcggcgttcg ccgcggccac tttcaggatt tcgccggtca
      421 tcactgctca ccttacgagc ggcgatgagg aagcggatgt ctgtgcgcta ggcgagtgcc
      481 ttggcgatgc caccggcggc cgggaagagc accaccacaa gaatcgacgg gaagatgaag
      541 agcaccaagg ggaacatgat cttcacgggc agcttggccg ccttctcccg ggcccattga
      601 cggcgacggt tgcgcagttc ggtggcctgc gtgtgcagga cacgtccgat cggcatgccc
      661 agtgcgtcgg cctgcgtgag cgccaggatg aagttggaca gttcggggac gtcggtgcga
      721 gcccggaggt tctgcatggc ctcacggcgg ctgagtccga gttccatctc gcgcaaggtg
      781 agccggaact cttcagccaa cgccgaatcg aagtggttgc acacgacgtc gagtgcggcc
      841 tcgaaaccga cgccggcctc gaccgagatg gccaacaggt cgaggcagtc gggtaggtcc
      901 ttgaaaatcg actcggctcg ctcggcgccc ttgcgcttca cgatggcgct cgggatgagc
      961 agaccggcgc cgggcatgag gatcaacacc agaagcgtca aacccggttt gccccccatg
     1021 gatgcgaatg cgatcgccgc gacaagtccg agcgcaccga gggcgacctg cagcgtgagc
     1081 agttcttccg gtgccacctt catgccggcg agcatgaggt cacggcgggc cttgtcgagc
     1141 aggccggctg gcgtcactcg gcgcaggagc gggctggtga cgcctcggag cgagcccagg
     1201 gtgcgagcga tgagcggctc gtgcagcttg cggacgaagt cgtcttcgat gtcgtcgtgg
     1261 tcctcgagtg aggcgaggta ctgcgacgcc gagttgccgg cgaagagtcg ctcgtagaag
     1321 ccggcggcga gcagaaccga gcccgccgtc gccatcagga tgaaaagcat gagtgaagga
     1381 gccatgttca gacctcgatc ttgaccattt tgaagatgat cccgacgccg acgcagatgg
     1441 agaccgcggt gaatgcgagg gcaaaaaggc ccgcaccgtg gaacagcggc ttggcgtagc
     1501 cagggttgag cgtttgcagc gcgaggagca ggaagatcgg catgccgccg agcacccagg
     1561 ccgaaatacg accttcagca gtaagcacgt cgacttcgcg gcgcacttcg tcgcgggccc
     1621 ggatgaagtc ggccagcgtg tgcagcagat cagcgagctt gccgcccacg gtctgctgga
     1681 tgcggatcgc ttgcacgacc cacgcgaggt cagccacgcc gacgcggtcc gacatgttcg
     1741 agagcgcatc gacgagcggc gcaccgaggc gaacctcgaa gaccacgcgt ccaaattcgt
     1801 ctgcgagcgg gggatcggct tcttcgcaca tcatctgaat ggagcgaagg aacgtgtgtc
     1861 cggcggacag cgacgacgcg atgagggaca acgcgtcggg cagctgacgg ctcagcgcct
     1921 tgcggcggcg ggcctggcgc agcttcggga acttcgcagt gccgaatacg gcgagtgcga
     1981 agaccatgcc gccgaggatg gggctgctgg tgatcgtggc aatcagcaac gcgccaaccg
     2041 tcgagacgca cgctgcgacc acgacgtact cgccggctcg cagcgggatg cgcgcccgct
     2101 cgagtgacgc ggcgagcgtg ccctgttcgt ccacttgagc gatcaggcgg cccgctagcg
     2161 acaccgctcc acgcgtgatg tggctcacgt cgacgacgga cttcgccgcc tcgatctggg
     2221 cttcctcttc ctcgccgaag gcgtcctcgc cgaaacgggc gaggtccatc agtgagttga
     2281 gcgccgccgc gcgctcacgc gcccgcagca gaaacccgac cacgatcgtg ctgaggccga
     2341 ggccgacgat gatgggaatc agaacccgca tcattggttg gcctcgactt tgggctcgat
     2401 atagccgtca tggccaggca gcgacgggcc gttgagccgg gtcgcccgcg cgacacgagg
     2461 ggcaccactc atcgcgcggg tgacacgaaa tacgtcggcg ggcagttcga cgccgcgctc
     2521 ggcgatgcgc tccacgaaac gtgggcgcag accggtcggc acgacgtcgc cggtggtccc
     2581 gttcggaccg tggcgcagct tgaacacgtc ctgcagcagg atggtgtcgc cttccatgcc
     2641 ctgtacttcg gtgacggcgg tcacgacacg acggccgtcg gggaaccgct cgagttggat
     2701 gatcacgtcg atcgcggcgt tgacctgctc acgaatcgcc cgcaccggca agtcgaagcc
     2761 cgccatgaga accatcgtct cgagacgaga cagtgcgtcg cgcggattgt tggcgtggac
     2821 ggtggtcatc gaaccttcat ggccggtgtt catggcctgc aacatgtcaa gcgcttcggc
     2881 gccgcggcac tcaccgacga tgatgcggtc ggggcgcata cgcagggcgt tcttgaccag
     2941 gtcacggatg cggacttcgc cgacgccctc tgcattcgaa ggacgagctt cgagcgagac
     3001 cacgtggggt ttctgcaact ggagctcggc cgagtcctcg atggtgatga tgcgctcttc
     3061 ctcggggatg aacgacgaca gcacgttgag cgtggtggtc ttaccagtgc cggtaccgcc
     3121 ggagacg
//
LOCUS       DATLCO010000283         4238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5026276, whole genome shotgun sequence.
ACCESSION   DATLCO010000283 DATLCO010000000
VERSION     DATLCO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5026276"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1809
                     /gene="purH"
                     /locus_tag="VMZ33_06520"
     CDS             262..1809
                     /gene="purH"
                     /locus_tag="VMZ33_06520"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HUR16920.1"
                     /translation="MRALLSVANRDGIAELARDLTTLGVELFATDGTLGALRDAGVEA
                     QPVTALTEKESLAGGQVRTFHPQIYAGILARRQVPEEITQLSEAGIGLIDLVIVNVKP
                     FAPQLGTRTVPLDEAIEMIDVGGTALITAAARNYASVAAVSHTRHYSQLLEELRAMGA
                     ISPEFRQRLAADALADVATYFAEVAGYLNHIAGTRFPEHLAIVVQKVRDFPYGENPHQ
                     QAAFYRETTHRYGSLADATQVQGDQPTLNDLLDLNAAYRIATDFTAPTVCIVKQTNPT
                     GIGSSDRLVEAYEKALAGDPVSAFGGVVALNREVDRETAAAIAENAYEAVVAPAFSAP
                     AREVLAEKEHLGVLAVPNQPFEGLTEYGISRLDFQRIDGGLLVETRDRSELDRSQLKV
                     VTRRRPTLEELTDLLFAWRTVRHVTSNAVVLARELAIVGVGAGQASRLTAVEIALHKA
                     ADRAKLSVLASDAYFPFADAIQLAAERGVTAIIQPGGSIRDEMAIEVADRHHMAMVFT
                     GRRHYRR"
     gene            1854..2831
                     /gene="glpX"
                     /locus_tag="VMZ33_06525"
     CDS             1854..2831
                     /gene="glpX"
                     /locus_tag="VMZ33_06525"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HUR16921.1"
                     /translation="METLLALEMVRVTEAAAIESARFMGRGDRHAADKAATEAMRKTF
                     DDVEMQGTIVIGEGERDKAPMLFIGEQVGIALPGQPEIDIAVDPLEGTNLVAMGVGNA
                     ITVLAASERGGLLNAPDTYFEKLCVGPIAAGQVDIRQSPEDNLNAIASALGRKVGDLT
                     IVILDRPRHADLIERVRKTGARIKLISDGDLSAAISCAVMGTGVHAVMGIGGAPEGVI
                     TAAALRCLGGEIQARFRFRNDEERARVERMGTAADEERVFTTNDLARGDYLVFAATGV
                     TEGDLLQGVRFFGGGARTHSLVMGFQSKQVRFVDTVHMFDRDKPPSVRL"
     gene            complement(2903..3499)
                     /gene="folK"
                     /locus_tag="VMZ33_06530"
     CDS             complement(2903..3499)
                     /gene="folK"
                     /locus_tag="VMZ33_06530"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HUR16922.1"
                     /translation="MTELMGSGESTSAGQGGRNGRVRAYVGLGSNVGDARATVRNAVR
                     AIAALPGVAVRAVSPAYLTRPVGITDQPQFLNAVVALDVPGGRTPGTGALALLTALKQ
                     IEHALGRQERQRWGPREIDLDLLVFGRHLIHVGPAEGLWLKVPHASAHERLFVLAPLA
                     DLAPRLRPPGWHETVETARRRRLETEGEEAIQPAGTIF"
     gene            3486..>4238
                     /locus_tag="VMZ33_06535"
     CDS             3486..>4238
                     /locus_tag="VMZ33_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16923.1"
                     /translation="MSSVTVLIFHRPPSSDDGPFTRALAEVRERLLAAHEAMFVAGGA
                     AEIRVIAEWRAGKTFGEVLANEAPARGGLVVLSSGAVPLLRLADAQRLVTVAGGRGRQ
                     ALTNNRYSSDICAVSDAATLHELPPLPSDNALPRWLEERAGYTVDELAARDRLALDLD
                     TPLDVAIAALARSCPTWLREAAQHNGFAVPRRDEIRALAANPHGELLVFGRAGSRTLR
                     WLERNVRCRVRFLAEERGMRAASPLAIGDPAAT"
BASE COUNT          697 a         1460 c         1399 g          682 t
ORIGIN      
        1 cgcgtccgca cgttgtacag aacgattgtg attaccaggc cgatcgcggc agcaatccag
       61 atcggattga agatgtcgcc gaagtggtcc ggtgtgaact gctgtccgag gggctcaaaa
      121 agcccgctgc ttcccatgcc cccgagaata acaaggcagc cggttctttc gccggtctat
      181 actccgccag ccgtgccgcg ctccgaaagt gagaggccgc gccggcgatc cacacggacg
      241 tgccacacgg aagagaggac cgtgcgagcc ctgctctcgg tcgccaatcg cgatggaatc
      301 gcggagttgg cgcgcgacct gacaacgctt ggggtcgagc tgtttgccac cgatggcacg
      361 ctcggcgcat tacgcgatgc cggcgttgag gcgcaacctg tcaccgccct gaccgagaag
      421 gaatcgctag ccggcggcca ggtgcgcaca ttccatcccc agatctacgc gggaatcctt
      481 gcccggcgcc aggtgccgga ggagatcacg cagctgagcg aggccggtat cggcttgatc
      541 gatctggtga tcgtcaacgt caagccgttc gcgccgcaac tcgggactag gacggtgccg
      601 ctcgacgagg cgatcgagat gatcgacgtg ggcggaaccg cgctgatcac ggcggcggcc
      661 cgtaactacg cgtccgttgc cgccgtttca cacacccggc actactcgca gctcctcgag
      721 gagctgcgcg ccatgggcgc catctcccct gagtttcgcc agcgcctcgc ggccgacgcc
      781 ctcgccgatg ttgccactta cttcgccgag gttgccggat acctcaacca catcgcgggg
      841 acgcgcttcc ctgagcatct cgccatcgtt gtccagaagg tgcgcgattt cccctacggc
      901 gagaaccccc accagcaggc tgccttttac cgcgagacga ctcatcgcta cggctcgctg
      961 gccgacgcca cgcaggttca aggcgatcag ccgaccctca acgacctgct cgacctcaat
     1021 gccgcctacc gcatcgcgac tgacttcacc gcgcccacgg tgtgcatcgt caagcagacc
     1081 aatccgacgg gcatcggctc gagtgatcgg ctggtggagg cctacgagaa ggcgctcgcc
     1141 ggcgacccgg tcagtgcctt cggcggcgtg gttgcgctca accgcgaagt ggaccgtgag
     1201 acggcggcgg caatagctga gaacgcctac gaagccgtcg tcgcgcccgc attctcggca
     1261 cctgcccggg aagtcctcgc ggagaaggag catctcggcg tactggcggt tccaaaccag
     1321 cccttcgagg gcctgacgga gtacggcatc tccaggctgg acttccagcg catcgatggc
     1381 ggcctcctcg ttgagacacg cgaccgttcg gagctcgacc gatcgcagct caaagtcgtc
     1441 acccgccgtc gaccgacgct ggaggagctg accgatctcc tgttcgcctg gcgaactgtc
     1501 cgtcacgtca cgagcaacgc ggtcgtgctg gcgcgtgagc tggcgattgt cggcgtgggc
     1561 gccggccagg ccagccgcct gaccgccgtc gaaattgcgc tccacaaggc ggccgaccgg
     1621 gcgaagctct ccgtcctcgc gtcagacgcc tatttcccgt tcgccgacgc catccagctc
     1681 gccgcagagc gcggcgttac ggcgatcatc cagcccggcg gctccatccg ggacgagatg
     1741 gccatagagg tggcagatcg ccatcacatg gcgatggttt tcaccgggcg gcggcactac
     1801 cggcgctgat ggacggccac tccccgtcac cagagagcat tcctgctcac tccatggaaa
     1861 ctctgctcgc cctcgagatg gtgcgagtca ctgaggcggc ggcaattgag tcggcgcgtt
     1921 tcatgggacg cggcgaccgg cacgcggcgg acaaggcggc cacggaggcg atgcgcaaaa
     1981 cgttcgacga cgtcgagatg cagggcacga tcgtcatcgg cgaaggggag cgcgacaagg
     2041 ctccgatgct cttcatcggc gagcaggtcg gaattgcgct gcccggccag cccgagatcg
     2101 acatcgcggt cgatcccctc gaaggcacaa acctcgtggc catgggcgtc ggcaacgcga
     2161 tcaccgtgct ggctgcttcg gagcgcggcg ggttgctcaa cgcgcccgac acctacttcg
     2221 agaagctgtg cgtgggaccg attgcggccg gccaggtcga catccgccag tcgcccgagg
     2281 acaacctgaa tgccatcgct tccgccctcg gacggaaggt gggggacctg acgatcgtca
     2341 ttctcgaccg gccgcggcac gccgacctga tcgagcgcgt tcgaaagacc ggcgcgcgca
     2401 tcaagctcat ctccgacggc gacctctcag cggcgatcag ctgcgcggtc atgggcacgg
     2461 gcgttcacgc cgtgatgggc atcggcggcg cgcccgaagg cgtgatcacg gctgccgccc
     2521 tgcgttgcct cggcggggaa atccaggcgc gcttcaggtt ccgcaacgac gaggaacggg
     2581 ccagggtcga gcgcatgggc actgccgcag acgaggagcg ggtcttcacg acgaacgacc
     2641 tggcgcgtgg cgactacctg gtgttcgcgg cgaccggcgt cacggagggc gacctcttgc
     2701 agggcgtgcg cttcttcggt ggcggtgccc gcacacactc gctggtcatg ggcttccaga
     2761 gcaagcaggt gcggttcgtc gacaccgtcc acatgttcga ccgcgacaag ccgccgagcg
     2821 tccgcctcta gtcgtgtttt cggccacaga cgcgtggccc ggtacgttca ggcatcgcgt
     2881 cgccagtggc gccattcggc ggtcagaaga tggttccagc gggctggatc gcctcttcac
     2941 cttcggtttc gaggcgtcgt cggcgagcag tctcaaccgt ctcatgccag cccggcgggc
     3001 ggagacgcgg ggccaggtcg gcgagcggcg ccaggacgaa aagccgttcg tgtgccgacg
     3061 cgtgcggcac cttcagccac agcccttcgg cgggcccgac atggatgagg tggcgaccaa
     3121 ataccagcag gtcgaggtcg atttcgcgcg gaccccagcg ctgccgctcc tgccgtccca
     3181 gcgcatgttc gatctgcttg agcgccgtaa ggagcgccag cgcccccgtc cccggtgtcc
     3241 ggccgccagg gacgtcgagg gcaacgaccg cgttgaggaa ctggggttgg tccgttatgc
     3301 cgaccgggcg cgtcaggtac gccggcgaga ccgcgcgcac ggccaccccg gggagcgcgg
     3361 caatcgcccg aacggcattc cgcacagtcg cgcgtgcgtc gccgacgttt gagccgagcc
     3421 ccacgtaggc tcggacgcgg ccgtttcggc cgccctgccc cgcgctcgta gactcgccgc
     3481 tgcccatgag ttcagtcacg gtcctcatct tccaccgtcc gccgtcatca gatgatgggc
     3541 cctttacgcg cgcactcgcc gaggtgcgtg aacggcttct cgcggcgcac gaggccatgt
     3601 ttgtcgcggg cggcgccgcc gagatccgcg tgatcgccga atggcgcgcc ggcaagacgt
     3661 tcggtgaggt gctcgcgaac gaagcgccgg cgcgtggcgg cctcgtggtg ctgagcagcg
     3721 gcgctgtacc actccttcga ctggccgatg cgcagcggct ggtcaccgtc gccggcggcc
     3781 gcggccggca ggcgctcacc aacaaccgct attcatccga catctgcgcg gtcagcgacg
     3841 cggccacttt gcacgagctg ccgccactcc ccagcgacaa cgctttgccg cgctggctgg
     3901 aagagcgcgc cggctacacc gttgacgagc tggcggcgcg cgaccgtctc gcccttgacc
     3961 tcgatacgcc actcgacgtg gccatcgcgg cacttgcgcg gtcatgccca acatggctgc
     4021 gcgaagcagc gcagcacaac gggttcgcgg ttccgcgccg cgacgagatc cgcgccttgg
     4081 ccgccaaccc gcatggtgag ctgctcgtct ttggtcgtgc cggctcgcgc actctgcggt
     4141 ggctggaacg caacgtccgc tgccgtgtca ggtttctggc cgaggagcgc ggcatgcgcg
     4201 cggcgagccc tctggcaatc ggcgaccctg cagccact
//
LOCUS       DATLCO010000284         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_572423, whole genome shotgun sequence.
ACCESSION   DATLCO010000284 DATLCO010000000
VERSION     DATLCO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572423"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VMZ33_06540"
     CDS             <1..642
                     /locus_tag="VMZ33_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUR16924.1"
                     /translation="RELCDRYGILLIADEVQSGAGRTGKMWAIEHWGVEPDILCAAKG
                     LGSGMPIGAMTARADLMSKWSKGHHGSTFGGNALSCAAALATIELLQDELIDNAAQRG
                     TQAMDGLRPLVSRHPELVKDVRGKGLMVGTQFDSGETAEAVQWAAFQRGLLVLEAGDD
                     AVRMSPPLIIDTSDVETAVRIFSESVDYVASDKQRAYREAMRWLDIHDTEVDG"
     gene            677..1588
                     /locus_tag="VMZ33_06545"
     CDS             677..1588
                     /locus_tag="VMZ33_06545"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HUR16925.1"
                     /translation="MSKVASMRDAVAELVRDGDTVAIEGFTHLIGFAAGHEIIRQRRR
                     DLTLCRLTPDLIYDQMIAAGVARKLVFSWLGNPGVGSLHAIRRVTEGDAASLELEEYS
                     HFGMVARYTAGAMNLPFFPIRSYDGTDLPVATPLIRPVESPYGDGPIYAVPPLKPDVT
                     IVHAQRADASGNTQVWGLLGCQKEAAFAAERVIVVVEELVAERVIRADPNRTILPGLV
                     VDAVVVEPFGAHPSYAQGYYDRDNAFYLEWEAISRDAASVAGWLDEWVHGVTGRSEYV
                     DKLGSARIDALRPEPALSEPVDYGSYR"
     gene            1620..2345
                     /locus_tag="VMZ33_06550"
     CDS             1620..2345
                     /locus_tag="VMZ33_06550"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HUR16926.1"
                     /translation="MMTAQPAFSATEMMIVAAARELAGQRVCFVGVGLPNIAVNLAQR
                     TVSPDLELVYEAGVFGARPQRLPLSIGDPTIVTGATSVTSMLELFGFYLQGGLIDVGF
                     LGAAQIDRHANINTTVIGDYSRPKTRLPGSGGACEIAQNAKSIFVIVRQSPRSFVERL
                     DFVTSVGSRVRVVVTDLAIFHLVDGELQLDSLHPGVSLEQVRSSMAWEPRLAASPAQT
                     PAPSNEELRMIREELDPTSVYTK"
BASE COUNT          430 a          844 c          847 g          456 t
ORIGIN      
        1 cgtgagctgt gcgatcgcta cggcatcctg ctcattgctg acgaagtcca gtcgggtgcc
       61 ggccggacgg gcaagatgtg ggcaatcgag cattggggtg tcgagcccga catcctgtgc
      121 gccgccaagg gcctcggctc agggatgccg attggcgcca tgaccgctcg cgccgacctg
      181 atgagcaagt ggtccaaggg ccaccacggc agcacgttcg gcggtaacgc cttgagctgt
      241 gctgcggcac tggcgacgat cgagctgttg caggacgagc tcatcgacaa tgccgcgcag
      301 cgtggcaccc aggcgatgga cggtctgcgc ccgctggtgt cgcgtcaccc cgagctggtc
      361 aaggacgtcc gcggcaaggg cctgatggtg ggcacgcagt tcgactcggg cgagaccgct
      421 gaggctgtcc agtgggcagc gttccagcgg ggcctgctgg tcctcgaggc aggcgacgac
      481 gcggtgcgca tgtcaccacc gctgatcatc gacacatctg acgtcgagac tgcggtgcgg
      541 atcttcagcg agtcagtcga ttacgtcgcc agcgacaagc agcgggccta ccgcgaggcc
      601 atgcgctggc tcgatatcca cgatacggag gtcgacggct aggggccact ccagccttcg
      661 cctccttccc gtcgagatga gcaaagtcgc gtccatgcgc gacgccgttg ccgagctggt
      721 ccgtgacggg gacaccgtcg cgatcgaggg ctttacgcac ctcatcgggt tcgccgcggg
      781 ccacgagatc atccgccagc gccgtcgtga cctaacgctg tgccggctca ccccggacct
      841 gatctacgac cagatgatcg ccgcgggcgt tgcgaggaaa ctcgtctttt catggctcgg
      901 taacccgggc gtcgggtcgc tccacgccat tcggcgagtg acggagggtg acgccgccag
      961 cctggagctg gaggagtaca gccacttcgg catggtcgcc cgctacacgg ccggagcaat
     1021 gaacctgccc ttcttcccga tccggtctta cgacggcacc gatctgcctg tcgcgacccc
     1081 cttgatccgg cccgtcgagt cgccgtacgg cgacgggccg atctacgccg taccgccgct
     1141 caagccggac gtgacgatcg tccacgccca gcgggcggat gccagtggca acacccaggt
     1201 ctgggggctg ctcggctgcc agaaggaagc cgcttttgcg gcggagcgcg tcatcgtcgt
     1261 cgtcgaggag ctcgtcgctg agcgcgtgat ccgcgccgac cccaaccgga cgatcttgcc
     1321 cggtctcgtc gtcgacgccg tggtggtcga gcccttcggc gcccacccct cgtacgccca
     1381 gggttattac gaccgtgaca acgcctttta cctcgagtgg gaggcaattt cgcgcgacgc
     1441 ggcgtcagtg gcaggctggt tggacgagtg ggtccacggc gtcaccgggc gttctgagta
     1501 cgtcgacaag ctgggtagcg ctcggatcga cgctcttcgc cctgagccag cgttgtccga
     1561 gccggttgat tacggctctt atcgctaact tcggcgagca agcgcgccac aatgacgtga
     1621 tgatgaccgc ccagcctgcg ttcagcgcga cagagatgat gatcgtcgcc gccgctcgcg
     1681 agctagcggg tcaacgcgtg tgcttcgtcg gcgtcgggct gccgaacatc gcagtcaacc
     1741 tcgcccagcg aaccgtctcc ccagatctgg agctggttta cgaggccggc gtcttcggcg
     1801 cccgcccaca gcggctcccg ttgtctatcg gcgaccccac gatcgtcacc ggcgcaacgt
     1861 cggtaacgag catgctcgag ctcttcggct tctacctcca gggcggtctc attgacgttg
     1921 ggttcctcgg agcagcgcag atcgatcgcc acgccaacat caatacgacg gtgatcgggg
     1981 actactcgcg gccgaagacg aggctgcccg gttcaggcgg cgcgtgcgag attgcccaga
     2041 acgcgaagtc gatcttcgtg atcgtccgac agagcccgcg ctcgttcgtc gagaggctgg
     2101 acttcgtgac gtctgttggc tcgcgcgtcc gcgtggtcgt caccgatctg gcaatcttcc
     2161 atcttgtcga cggtgagctg cagctggact cgctgcaccc cggcgtgtcg ctggagcaag
     2221 ttcggtcgtc gatggcttgg gagccacgcc tggccgcgtc cccggcgcaa acgccggccc
     2281 cgagtaacga ggaattgcgc atgatccgcg aggagctcga tccaaccagc gtctatacga
     2341 agtaggcaac gaccctcgag tcgccaatat cgctcacatg ctcagcccgg gcgtgccggg
     2401 cacagctccc agcttccagg agttcgtctg ggggtcggta gcgcctctgg atatcgccac
     2461 tgccgacgcc gttggagggc cggctattgg cggcgcgagg aaaccagaag tagcaggccg
     2521 acgccgatga tcacgatcgg ccagataaca gacagctcgg ggatgttcgc cagctga
//
LOCUS       DATLCO010000285         5585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6997519, whole genome shotgun sequence.
ACCESSION   DATLCO010000285 DATLCO010000000
VERSION     DATLCO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6997519"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="VMZ33_06555"
     CDS             complement(<1..2067)
                     /locus_tag="VMZ33_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUR16927.1"
                     /translation="MNLPFTIARPELLLLGIALLVITLGLSLAARHHLAKSRRRLSLV
                     LRAVILSCLALALAGLAIVWPVDRLTTVFVVDMSDSVGQQGRESSLAYLRDSLALRPE
                     GDKAAIVAFGTEALVERLPAELTDLDRIASVPATSATDIGGALRLAAALFPDDTQKRI
                     VLVTDGNDTTGRGQSEAALAAARGVQIETFVVGLGASDETIVQRVHSPATARVGEDIE
                     IEVTVTSTVAQPATVRLFGDGRQIGVQDVNLTAGVNSVVFSTEATVAGFHTFRARVEA
                     EHDTFTQNNYGDSHTIVKGDPRILLISGSAEAGGNVRDALEAENQDVTEVTPESSPTD
                     LTGYASFDAVVLADVAATRLGTARMTALQVYTRDLGRGLVMIGGPSSYGAGGYKRTPL
                     EEALPVDMDVRDRDRQPDIALVVVIDQSGSMDACHCNTPNRDNGVAITGIPKVDIGKE
                     AILRAVSALTERDEFGVVSFNENAHWVIRTAPLGNVGNVEEQIAGIRPDGQTNIFAGL
                     SEAVASLEQTSATRRHIVLFTDGWSSSGQYDDLIRRMSAAGITLSTVGAGGGGANDFL
                     SGLAERGGGRFYPAPDPAAIPDIFLKETQQVSGQQIVEETFFPILTSSSPILNGLEDG
                     LPQLRGYNGTTPKSAAQIVLVSTRDDPVLAQWQYGLGRSVAWTSDAQGRWATNWVAWT
                     GFNQFFS"
     gene            complement(2075..4147)
                     /locus_tag="VMZ33_06560"
     CDS             complement(2075..4147)
                     /locus_tag="VMZ33_06560"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HUR16928.1"
                     /translation="MGFLAPLALIGLISVPIIVAFYMLRLRRPERAVSSTFLWQQLVR
                     DVEANAPWQKLRRSLLLLLQILLAIIFAVLVARPFAEHPAGLARDLVLVIDASASMSA
                     TDDFPTRLEAAKRLAIEQMGEVPSDGRVSVVSATETARVVANEATDRGRISRAIESIE
                     ASTGASNLTDALKLAASLAQRARGAEILVITDDAGSVLPDVSLEVPVRVATVGRERNN
                     QAIAALAVRSDSSGLRRTLFVSVANYSAEAVGRRLEILADNTSVTARDLALQPLSRAD
                     VVIDELPPGATVVQARITAPAGSGSPVSSATDYLPLDDSAWAIVPPDRLRKVLLVGPG
                     NVYLQNAFSLLPNVELYGATAAEYPNTTGMDLYDLIVFDGFLPPELPNKPILAFAPPA
                     TSDLGVVSGTLEEFGMGQPSADDPLLRGVDLTRLHIAKTQNMELPDWGRAVVPGSPGP
                     LVYAGLRQGLATVVFAFDIRQSDLPLQIAWPILVSNVAGELLGVGPQQNFDPLPPASP
                     VEIPITQDATGVRITLPDGTVEEIAPGATGASTVSFVNTRQLGVYRVEVIKAPAPSAS
                     PAATPGATPTPSTTPGASGSPQPAPAADDPNMFAVDLFAPDESNIRPGDGARISSLGT
                     DLPPAEQQTGTARDEFWPLVAALALAFLLVEWIVYERDGARRILNAIRGANPFANRGR
                     ATGRKGAG"
     gene            complement(4165..5085)
                     /locus_tag="VMZ33_06565"
     CDS             complement(4165..5085)
                     /locus_tag="VMZ33_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUR16929.1"
                     /translation="MTAPTRDLLPSAVDPTVFDEAFLRQLERLMLVTKKAVHGGMKGV
                     RRSVKRGQSVEFTDYRNYTLGDDLRALDWNLYARLEKLFVKLFIEEEDVNVHILLDAS
                     ASMETGSPDKLLFGKRAAAALAYVGLASYDRISMAVLQGRAARRFPPVRGTGRIFQVL
                     ADLSAVKPSSGPTDLAAATRHYAAQITQRGPLILISDLFDANVERAISELAGTRCDVA
                     VLHTLSTDELDPPLEGDLRLIDKETGESVNVTADLYTLDAYKARLAGWQQRLDEVSSK
                     RRVAYTPIPTTLPLADLVFAELRRRNVVAA"
BASE COUNT          924 a         1784 c         1992 g          885 t
ORIGIN      
        1 cgagaagaac tggttgaagc ccgtccaggc cacccagttg gtcgcccatc gaccctgggc
       61 atccgacgtc cacgccacgg accggccgag gccgtactgc cattgcgcca ggacggggtc
      121 atcgcgggtg gacacgagaa caatttgcgc cgcggacttg ggtgtcgtcc cgttgtagcc
      181 gcgcagctgc ggcagcccgt cctccaggcc gttgaggatc ggcgaggacg aggtcaggat
      241 ggggaagaag gtctcctcga cgatctgctg gccggaaacc tgctgtgtct ccttgaggaa
      301 tatgtcgggg atggcggcag gatccggtgc cgggtagaag cggccgccgc cgcgctcggc
      361 gaggccggac aggaagtcgt tcgccccacc accgccggca ccaaccgtgg agagggtgat
      421 ccccgccgcc gacatgcgcc ggatgaggtc gtcgtactgg ccgctgctcg accagccgtc
      481 cgtgaacagc acgatgtgtc gccgcgttgc cgacgtctgc tcgagcgagg ccaccgcctc
      541 tgacaggccg gcaaaaatgt tggtctggcc atcggggcgg atgccggcga tctgctcctc
      601 gacattgcca acgttgccca ggggcgccgt gcggatgacc cagtgcgcgt tctcgttgaa
      661 cgacacgacg ccaaactcat cgcgctccgt caaggcagaa acggcgcgga ggatcgcctc
      721 cttgcctata tcgaccttgg gaatgccggt gatcgccacg ccgttgtcgc ggttgggcgt
      781 gttgcagtgg caggcgtcca tcgagcccga ctggtcaatg accactacga gcgcgatatc
      841 gggctgccga tcacggtcgc ggacgtccat gtcgaccgga agggcttcct caagaggagt
      901 gcgcttgtag ccgccggcgc cgtacgagct ggggccgccg atcatgacca ggccgcggcc
      961 gaggtcacgc gtgtatacct gcagcgccgt catgcgcgcg gtgcccaggc gggtcgctgc
     1021 cacgtcggcg agaaccaccg cgtcgaacga ggcatagcca gtgaggtccg tcggggagct
     1081 ctcgggcgtg acctcggtga cgtcctgatt ctcggcctcg agcgcgtcgc ggacgttgcc
     1141 gccggcttcc gcgctgccgc tgatcaggag gatccgcgga tcgcccttca cgatggtgtg
     1201 tgagtcgccg tagttgttct gggtaaacgt gtcgtgctcg gcctcaaccc gtgctcggaa
     1261 ggtgtggaac ccggccacgg tggcctcagt cgagaacaca actgagttca cgcccgccgt
     1321 caggttgacg tcctggacgc cgatttgccg gccatcccca aacaggcgca ccgtcgcggg
     1381 ctgggcgacg gtggaggtga cagtgacctc gatctcgatg tcctcgccca cgcgcgcagt
     1441 ggcgggggag tggacgcgtt ggacgatggt ctcatcggac gcgccgaggc cgacgacgaa
     1501 cgtttctatc tggacgccac gcgcagccgc gagtgcggcc tctgattggc cgcggccggt
     1561 ggtgtcgttg ccgtccgtga cgagcacgat gcgcttctgc gtgtcgtcgg gaaacagcgc
     1621 ggccgccagg cgcagtgcgc ctccgatgtc cgtggccgac gtcgccggca cggacgcgat
     1681 gcgatcgagg tcggtcagct ccgccggcag gcgctctacg agggcctcag tgccaaatgc
     1741 cacgatcgcc gccttgtcgc cctcgggccg cagcgcgagc gagtcgcgca ggtaggcgag
     1801 cgacgactcg cgcccctgtt ggccgacgct gtcggacatg tcgaccacga agacggtggt
     1861 caaccgatcg accggccaga cgatggcgag gccggcgagc gcaagcgcaa ggcagctgag
     1921 gatgaccgcc cgaagaacaa gcgacagtcg ccgccggctc ttcgccaggt gatggcgcgc
     1981 tgccaggctg aggccgagtg tgatgacgag cagcgcgatg ccgagcaaca gcagctcggg
     2041 gcgcgcgatg gtgaagggca ggttcatcgg gcgctcatcc agcgcccttg cggccggtgg
     2101 cgcgtccgcg gttcgcgaac gggttcgcgc cgcgaatcgc gttcaggatg cgtcgtgcgc
     2161 cgtcgcgctc gtacacgatc cactccacga ggaggaaggc aagtgcgagc gcggcgacaa
     2221 gcggccagaa ctcgtcgcgg gcggttccgg tctgctgctc cgcgggcggc aggtccgtgc
     2281 cgagcgacga gatgcgcgca ccgtcgcccg gccggatgtt ggactcgtca ggtgcgaaga
     2341 ggtccaccgc gaacatgttc ggatcgtcag ccgcgggcgc gggctgaggc gaaccgctgg
     2401 cgcctggtgt ggtcgagggc gtgggggtgg caccgggagt cgccgcgggc gaggcactcg
     2461 gggcgggtgc cttaattact tcgaccctat atacgccgag ctgacgcgtg ttcacgaacg
     2521 aaacagtcga ggcgccggtc gcgcctggcg caatctcctc gaccgtgccg tcgggcaggg
     2581 tgatgcggac gccggtggcg tcctgagtga tcgggatctc aacgggcgac gcgggcggca
     2641 gcgggtcgaa gttctgctgc ggtccgacgc cgagcagctc tccggcgacg ttcgagacga
     2701 ggatgggcca cgcgatctgg agcgggaggt cgctctggcg gatgtcgaac gcgaagacga
     2761 cggtcgccaa cccctggcgc aacccggcgt acaccagcgg accgggcgac ccgggtacga
     2821 cggcgcgacc ccagtcgggc agctccatgt tctgtgtctt ggcgatgtgc agccgcgtca
     2881 ggtccacacc tcggagaagc gggtcgtcgg cactgggttg gcccatgccg aactcctcga
     2941 gcgtgccact gaccaccccc agatcgctgg ttgcgggcgg ggcaaaggca aggatcggtt
     3001 tgttggggag ctcgggcggt aggaacccgt cgaacacaat gaggtcgtag aggtccatgc
     3061 cggtcgtgtt cgggtactcg gccgccgtgg cgccgtaaag ctccacgttg ggcagcagcg
     3121 agaaggcgtt ctgcagatac acgttgccgg ggccgactag caggaccttc cgcagacgat
     3181 cgggcggtac gatcgcccac gccgagtcgt cgagcggcag gtagtccgtc gcgctgctga
     3241 ccggtgaccc gcttcccgcc ggcgccgtga tgcgtgcttg aaccactgtc gcgccggggg
     3301 gcaattcgtc gatcacgacg tccgcccggg acagcggctg cagcgcgaga tcgcgggcgg
     3361 tgaccgacgt gttgtcggcg agaatttcca gtcgacggcc gacagcctca gccgagtagt
     3421 tggccacgct cacgaaaagt gttcgccgca agccgctgga gtccgaacgg acggccagcg
     3481 cggcgatggc ctggttgttg cgctcacggc caacggtcgc aacacgcacc ggcacctcga
     3541 gggagacgtc tggaagcacc gaacccgcgt cgtcggtgat gacgagaatc tccgccccgc
     3601 gggcgcgctg cgccagtgag gctgcgagct tgagcgcgtc ggtgaggttg gaagctccgg
     3661 tggacgcctc gatcgattcg atagcgcgcg agattcgtcc gcgatcggtc gcctcgttgg
     3721 caacgacgcg cgccgtctcc gttgccgaga ccacgctcac ccggccatcg gacggcactt
     3781 cgcccatctg ctcgatggcc agtcgcttgg cagcctcaag ccgggtcggg aagtcgtcgg
     3841 tcgccgacat cgacgccgaa gcgtcaatga cgagcaccag gtcgcgcgcc agccccgcgg
     3901 gatgctcggc gaagggccgc gcaacgagga cggcgaagat gatcgcgagc aggatctgaa
     3961 gaaggagaag cagggaccgg cgcagcttct gccacggcgc gtttgcctcg acgtcgcgga
     4021 cgagctgctg ccacaggaac gtgctgctga ccgctcgttc gggtcggcgc agccggagca
     4081 tgtagaacgc gacgatgatc gggacggaga tcagcccgat cagcgccaag ggcgcaagga
     4141 atcccacggt ttgcccgcgc ccgcctaggc cgccacgacg ttgcggcgtc gcagctcggc
     4201 gaagacgagg tctgccagcg gcagagtcgt cggaatcggc gtgtacgcaa cccggcgctt
     4261 gctggaaacc tcgtcgaggc gctgctgcca gccggcaagt cgggccttgt aggcgtcgag
     4321 cgtgtagagg tcagcggtga cgttgaccga ctcgccggtc tccttgtcga tcaagcgcag
     4381 gtcgccttcc agcggcgggt ccagctcgtc agtcgagagc gtatgcagga cggcgacgtc
     4441 gcagcgagtc ccggcaagct cgctgattgc gcgctcgacg ttggcatcga acaggtcgct
     4501 gatcaggatg agtgggccgc gttgcgtgat ctgcgccgcg tagtggcgcg tcgctgccgc
     4561 gaggtccgtc ggacccgacg acggcttgac cgcagaaagg tcggcgagca cctggaagat
     4621 gcgtccggtg ccgcgaaccg gcgggaagcg acgcgcggca cggccctgca gcacggccat
     4681 cgagattctg tcgtacgagg ccaggcccac gtaggccagg gcagcggccg cgcgcttgcc
     4741 gaagagcagc ttgtccggtg agccggtctc catggaagcc gacgcgtcga gcaggatgtg
     4801 gacgttgacg tcctcttcct cgatgaaaag cttgacgaac agcttttcca gccgggcata
     4861 gaggttccag tcgagcgcgc gcaggtcgtc gccgagggtg tagttgcggt agtcggtgaa
     4921 ttcgaccgac tggccgcgct tgacgctgcg acggacgccc ttcatgccgc cgtggacggc
     4981 cttcttcgtg accagcatga gtcgctcgag ctgccggaga aaggcctcgt cgaagacagt
     5041 cgggtcgacg gcagacggga gaagatcgcg tgtcggtgcg gtcatcgccg cgccactgcc
     5101 gtggccaggc tctgccgacg ccgagctttc gcgtccgggt cctgggttgc ccagcacttg
     5161 tccaatctga ttggagacaa aggcccggaa tcgcggccgc gcaggtaaaa acgtgacgta
     5221 agtcggcgag atccgagggc agacacgctg ggcagcacgc gagtcgcagc ctttcgcacc
     5281 cgcaacctgg cggaaatcgt cacttcaggg tcgctccgac aaatctgatt ggaaaatggc
     5341 taggcggcgc gctcttgggc gtccccagtc ccgcggcgaa acgagatttg tcgtcgcagc
     5401 ggccttcgcg ccgctgctac gcgcccccgg cgggccactg cgctgcagtt gcccgtcgcg
     5461 tgtatccatc agtcgaccga aggcgccggc accgcatcga tcaccgcgcg cacgatcgcg
     5521 tcgctggtga ttccctcggc ctctccctcg aagttgcgga tcacgcggtg ccggagcgcg
     5581 ggcaa
//
LOCUS       DATLCO010000286         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_579766, whole genome shotgun sequence.
ACCESSION   DATLCO010000286 DATLCO010000000
VERSION     DATLCO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579766"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VMZ33_06570"
     CDS             <1..472
                     /locus_tag="VMZ33_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16930.1"
                     /translation="VGARPFLIAYNINLDTDDVDLAKSIAKEIRESSGGLPRVQGLGL
                     YLDDLRCAQVSMNLLDFNVTPIWKVWEAVESRAASAGVTLRESELIGLAPVAALVDVA
                     DHIGVDSAAVVEARITDAARWLKIRDFEPSMALELRLAAAQAASDDAARDALTL"
     gene            469..1782
                     /gene="hutI"
                     /locus_tag="VMZ33_06575"
     CDS             469..1782
                     /gene="hutI"
                     /locus_tag="VMZ33_06575"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HUR16931.1"
                     /translation="MNDERPGLLVHGASAVATLAGGMRRGAGQDDVALLSADGVAIGS
                     YEGRIVAVGQLNDVEARLSQHGIATSALTRLDARGGLVTPGLIDPHTHLLFGGTRHAE
                     VGLRQRGLGYLEILVAGGGILQTVRMTRAASDDELLDHGRRWLREMMTHGVTTVEAKS
                     GYGLELDAELRLLSLLGKLGDEGPVEILPTFLGAHAVPPEYRDGPAPAADYLREVIEV
                     QLPEVARQGIARFVDVFCEPGVFTVEQARSLLRAARDRGMDLRLHADQINAGGGAELA
                     VEVGARSADHLGAITDAGIAALAVAADAGRPVVATLLPIASFYLGEEHDAPARQLIDA
                     RVPVALGTDFNPGTSPAPNVQLALAFAVHRLKMSASEALAALTINAAAALGIADTHGS
                     LEEGKHADLVVWKINSHELLPYWLGANLASAVVKRGRVVYVAPAD"
     gene            complement(1779..>2205)
                     /locus_tag="VMZ33_06580"
     CDS             complement(1779..>2205)
                     /locus_tag="VMZ33_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR16932.1"
                     /translation="DVHEILVTGIRTFRDEQHGAERGYNMCFVYELDNVHIVHLGDVG
                     HLLSEGDLGEIGTVDVVCVPVGGSLSAAKAAELVAQLDANLVVPMPLDPDGAGGEELS
                     KFFHEMSVTHVEPVPKLTVSVSSLPTETTVVVLEPRGKS"
BASE COUNT          344 a          744 c          754 g          363 t
ORIGIN      
        1 agtgggcgcc cggccgttcc tcatcgccta caacatcaat ctcgacaccg atgacgtgga
       61 tctggcgaag tcaatcgcca aggagattcg cgagagcagt ggtggcctgc cgcgcgtcca
      121 gggtctgggc ctgtacctgg acgacctgcg ctgcgcccag gtatcgatga acctgctcga
      181 cttcaacgtc acgccaatct ggaaggtgtg ggaggccgtc gagagccggg ccgcctcggc
      241 aggtgtgacg ctgcgggaat ccgagttgat cgggctggcg cccgtggccg cgctcgtcga
      301 cgtggccgat cacatcggcg tggattctgc cgctgtggtc gaggcccgga tcaccgacgc
      361 ggctcggtgg ctcaagattc gcgacttcga gccctcgatg gcgctggagc tgcgcctcgc
      421 cgctgcccag gccgcgtccg acgacgcggc gcgtgatgcg ctgacgttgt gaacgacgag
      481 cggccggggc tgcttgtcca cggcgcctcg gccgtggcca cgctcgctgg cgggatgcgc
      541 cgcggggccg gccaggatga tgtcgcactt ctgtcagcag acggtgttgc gatcggctcg
      601 tacgaaggca ggatcgtggc cgttgggcag ctgaatgatg tcgaagcgag gctgtcccag
      661 cacggcatcg cgacttcggc gctgacccgg ctcgacgctc gcggcggact ggtcaccccc
      721 ggcctgatcg acccccacac ccatctgctc ttcggcggga cgcgccacgc cgaggtcggg
      781 cttcgccagc gcggactcgg ctatctcgag atcctcgtcg cgggcggcgg catcctccag
      841 acagtgcgga tgacccgcgc cgccagcgat gacgagctcc tcgaccacgg tcgccgctgg
      901 ctgcgcgaga tgatgactca cggcgtcacg acagtcgagg cgaaatctgg ttacggcctg
      961 gagctcgatg ccgagctgcg gctgctgagc ctcctcggca agctgggcga cgaggggccg
     1021 gtcgaaatcc tgcccacgtt cctcggcgcc cacgccgtgc cacccgaata tcgggacggg
     1081 cctgcgccgg cggccgacta cctgcgcgag gtgattgaag tccagctgcc tgaggtcgca
     1141 cgacagggaa tcgcgcggtt cgtcgacgtc ttctgcgagc cgggcgtgtt cactgtggag
     1201 caggcacgct cgttgttgcg cgccgcacgc gaccgcggaa tggacctgcg gctccacgcc
     1261 gaccagatca acgctggggg tggcgctgag ctggcggttg aggtcggcgc acgctctgcc
     1321 gatcacctgg gcgccatcac ggacgccggg atcgccgcgc ttgcggtcgc cgcggatgcc
     1381 ggccgacccg tcgtggcgac gctgctgccg atcgcgagct tctatctcgg cgaggaacat
     1441 gatgcgccgg cgcgacagct cattgacgcg cgcgtgccgg tcgccctggg gaccgacttc
     1501 aacccgggca cctcgcccgc cccaaacgtg cagctggcgc ttgccttcgc cgtccatcgc
     1561 ttgaaaatgt cggcgagcga ggcgctggcg gcgctgacca tcaacgcagc ggctgcgctc
     1621 gggatcgcgg acacgcacgg ctccctggag gaaggcaagc acgccgatct ggtggtgtgg
     1681 aaaatcaatt cccacgagct gctgccctat tggctcggcg caaacctcgc atcggccgta
     1741 gtgaagcggg gccgcgtcgt ctacgtggcc ccggcggact agctcttgcc gcgcggctcc
     1801 aggacgacga ccgtcgtctc agtcgggaga gagctcacgc tcaccgtcag cttcggcaca
     1861 ggctcaacgt gcgtgacgct catctcatgg aagaacttgc tgagctcctc gcctccggcg
     1921 ccatcggggt cgagcggcat cggcaccacc agattcgcgt ccagctgcgc aaccagctcc
     1981 gcggccttgg cggctgagag cgacccaccc acgggaacgc acacaacgtc gaccgtgccg
     2041 atctcgccca ggtcgccctc ggagagaagg tgcccgacgt cgcccaggtg gacgatgtgc
     2101 acgttgtcga gctcgtagac gaagcacatg ttgtagccac gttcggcgcc gtgttgctca
     2161 tctcggaacg tacggatccc ggtgacaagg atctcgtgaa cgtcg
//
LOCUS       DATLCO010000287         2815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4280476, whole genome shotgun sequence.
ACCESSION   DATLCO010000287 DATLCO010000000
VERSION     DATLCO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4280476"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /gene="mraZ"
                     /locus_tag="VMZ33_06585"
     CDS             <1..511
                     /gene="mraZ"
                     /locus_tag="VMZ33_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HUR16933.1"
                     /translation="RLGVGHDPAVERPTVFPATRHRPSRGSRVFTGEYRHAIDAKGRV
                     AVPARFRAELAGGGFVTRWIDNCVGIFPKQEWEKVTSRVADLPFSDAGSRVFTRFMFA
                     GAFEFQPDTQGRLLVPPVLREFAALSEETVVVGARDHIELWAPDRWAAYSADMTGDSL
                     AQRIQDLGI"
     gene            520..1509
                     /gene="rsmH"
                     /locus_tag="VMZ33_06590"
     CDS             520..1509
                     /gene="rsmH"
                     /locus_tag="VMZ33_06590"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HUR16934.1"
                     /translation="MSLQGPKGPQSTAVESEEGHVPVMVEEVLKSLAPAPGSFQIDAT
                     VGGGGHSLRILEANSPDGRLLGLDADGAAIARTSNRLERFGDRVTLTQTNFEHLADAA
                     AAANFVPADGVLLDLGLSSYQLADDERAFSFRAQGPLDMRFDTSRGVPASDLVNTYDE
                     QQLTELFRKYGEEPHARRIARAIVHRRDQGPITTGTQLAEVVAAAAPAPPRGRRRVHP
                     ATRVFQALRIAVNRELETLPSALEAAVNVLGEGGRLAVLSYHSLEDRIVKRFIATERR
                     GCICPPEAPVCICGRQPRLAPVGPQPRRPTDAEIATNPRARSALLRAARKLAA"
     gene            1703..2104
                     /locus_tag="VMZ33_06595"
     CDS             1703..2104
                     /locus_tag="VMZ33_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16935.1"
                     /translation="MTVLSGARPFPVGARVLPRAESGSRQAARPHTTARRRVSSPARA
                     GRRAQPFGGLIAIVLVALVIGLIYLAQMVRLAQISYEIDTLGSTRDDLSRQVQTLETS
                     VLRYGTESMVLERAQQIGLGQLETRVRLPAR"
     gene            2108..>2815
                     /locus_tag="VMZ33_06600"
     CDS             2108..>2815
                     /locus_tag="VMZ33_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16936.1"
                     /translation="MLGRTDSPRRLLALLLVLGIFACALGGRLAYWQIGQVDELRTRA
                     GNQSVAGQTEPIRRGEILDVNGAVLATTAYRDRLVAYPDMLAAADRDAVVAQVAEILG
                     LDSAGRDDLTAKFAAGAPYAVLSRQLTPEQSQKVRDGLDTDLYALGLEPQAIRFYPND
                     GGSPDTTLASQLLGFVTADGQGRYGIEQYRQDLLAGVDGATANLAADSLAVPTIGDDV
                     MLTIDASLQLRLEKELYA"
BASE COUNT          464 a          971 c          905 g          475 t
ORIGIN      
        1 tcgcctaggg gtagggcacg accccgcggt ggaacgccca accgtcttcc ccgcgacccg
       61 gcacaggccg agcagaggta gtcgtgtgtt caccggcgag taccgccacg cgatcgacgc
      121 caaggggcgc gttgccgtcc ctgcgcgatt tcgggctgag ctcgcgggag gtggattcgt
      181 gacccgctgg atcgacaact gtgtcggcat cttcccgaag caggagtggg agaaggtcac
      241 gagccgtgtc gctgatcttc ccttctccga cgcaggctcc cgagtcttca cccgtttcat
      301 gttcgccgga gcgttcgagt tccagcccga cacccagggt cggttgctcg tcccaccggt
      361 gctgcgcgag ttcgcagccc tgagcgaaga gacggttgtt gtcggagcgc gcgatcacat
      421 cgagctctgg gcgcccgacc ggtgggcggc ttacagcgcc gatatgacgg gtgattcact
      481 cgcccagcgc atccaggacc tcgggatcta gcgcaccaaa tgagcctcca gggtccgaag
      541 ggaccgcaat cgacggccgt ggaatcggag gaagggcacg tccccgtgat ggtggaagag
      601 gtattgaagt ctcttgcgcc cgctccgggg agcttccaaa ttgatgcaac tgtcggtggc
      661 ggagggcact cccttcgaat cctggaggcc aactcacctg atggccgcct cctcggcctg
      721 gatgctgatg gggcggccat cgccagaacc agcaatcggc tggagcgttt cggcgatcgg
      781 gtgacgttga cccaaacgaa tttcgagcac cttgccgacg cggcggcagc ggcgaacttt
      841 gttccggctg atggcgtgct actcgacttg ggcttgagct cctaccagct cgccgacgac
      901 gagcgcgcct tcagcttccg cgcccagggc ccgctcgaca tgcgtttcga cacaagccgg
      961 ggagtgccgg cgagtgacct cgtcaacacc tacgacgagc agcagctgac tgagctgttt
     1021 cgcaagtacg gtgaggagcc ccacgcacgt cgcatcgcgc gggcgatcgt tcaccgccgc
     1081 gaccagggac cgataaccac gggcacgcag ctcgctgaag tggtggccgc cgcggcaccc
     1141 gcgccccctc gcggcaggcg gcgggttcac ccggcgacgc gcgtgttcca ggcgcttcgc
     1201 atcgcggtca accgcgaact agagactctc ccttccgctc ttgaggcggc cgtcaacgtg
     1261 ctgggcgaag gcggccgcct cgcggttctc tcgtatcact cgctcgaaga tcgcatcgtc
     1321 aagcgcttca tcgccaccga gcggcgcggc tgtatctgcc cgcccgaagc gccggtctgc
     1381 atctgtggcc gccagccgcg cctggcgcca gttggcccac agccgcgccg accgaccgat
     1441 gcggagatcg cgacgaaccc ccgggcgcgc agtgccttgc ttcgcgccgc acgaaagctg
     1501 gccgcatgag ccgccgccac aacgcctcgc gccggcgaac ctacacgcgc cgtcagcacg
     1561 aaatacgaga gcgacggccg gagaccgccg gcaccggcga ctggcgcgaa accggtgacc
     1621 tcgatgaatt tgacgtgtct caagacttct cactcgacct cttgtcgcct cccgcaagaa
     1681 cccggttctc cggtgcgcac tcgtgaccgt tctctcgggc gcgcggcctt ttcccgtcgg
     1741 tgcgcgtgtc ctcccgcgtg ccgagtcggg gtcacgacag gccgcgcgcc cacacaccac
     1801 cgcgcgtcgc cgcgtttcga gcccagctcg tgccggtcgc cgcgctcagc ccttcggcgg
     1861 attgattgca atcgtgctgg tggcactcgt catcgggctc atctacctcg cgcagatggt
     1921 tcgccttgcc cagatctcgt acgagatcga caccctgggc agcacgcgcg atgacctgtc
     1981 ccgccaggtt cagacactcg agacgagcgt gctgcgctac gggaccgagt cgatggtcct
     2041 cgagcgcgcg cagcagatcg gactcggcca actcgagacc agggttagat tgccggcccg
     2101 ctgaccgatg ctcggtcgga ccgacagccc acggcggctg ctagcgcttc tcctcgtcct
     2161 cggtatcttt gcctgcgcgc tcggaggccg gcttgcctat tggcagatcg gccaggtcga
     2221 tgagctacgg acgcgcgccg gaaaccagtc ggtggcgggc cagactgaac ccattcgacg
     2281 cggcgagatc ctcgacgtga acggcgcggt cctggccacg accgcctatc gggatcgcct
     2341 cgttgcatac ccggacatgc tcgctgccgc ggatcgggat gccgtcgtgg cccaggttgc
     2401 ggagatcctc ggcctggatt cggccggcag ggacgacctg accgcgaaat ttgctgccgg
     2461 agcgccatac gccgtcctat cccgccagct gacgcctgag cagagccaga aggtgcgcga
     2521 tgggcttgac accgatctgt acgcgctcgg tctcgagcca caggcaattc gcttctaccc
     2581 gaatgacggt ggatcgcccg ataccacgct ggcttcgcaa ctgctcggtt tcgtgaccgc
     2641 cgacggccag ggccgctacg gcatcgagca gtatcgccag gatctgctcg cgggcgtcga
     2701 cggcgcgacc gcaaacctgg cggccgacag cctcgccgta ccgactatcg gcgatgacgt
     2761 gatgctgacg atcgacgcca gcctgcagct gcgactcgaa aaggagctgt acgca
//
LOCUS       DATLCO010000288         6793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4283781, whole genome shotgun sequence.
ACCESSION   DATLCO010000288 DATLCO010000000
VERSION     DATLCO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4283781"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="mnmA"
                     /locus_tag="VMZ33_06605"
     CDS             complement(<1..832)
                     /gene="mnmA"
                     /locus_tag="VMZ33_06605"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HUR16937.1"
                     /translation="MRERVLVAMSGGVDSSVAAALLAEEGVETVGVWMRLHDVADAYS
                     EFKKSCCSLDAADDARRVAGQLGIPFYVLNLEREFGAGVLQPFLDAYLDGRTPSPCVD
                     CNSYVKFGALLGKALRHYDCTAVATGHYARVDAHEDPDAPGGRRYRLLKGLDTDKDQS
                     YFLYGLRQEQLGHVRFPLGAMTKAEVRDVARRHGLATAEKPESQEICFVPGGDYRTAL
                     KERAGWHEEAGPLIDVDGTRVGDHRGAAAYTIGQRQGLGVALGEPRFVNTIDAATNIV
                     T"
     gene            complement(846..2030)
                     /locus_tag="VMZ33_06610"
     CDS             complement(846..2030)
                     /locus_tag="VMZ33_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HUR16938.1"
                     /translation="MPVYLDHAATTPLRREALDAMMPWLTDGFGNPSSIHAIGRRARA
                     ALDEARERTAAALNAEPREIIFTGGGTESINLALKGIAWAGKARGNRLVTSAVEHHAT
                     MYALRHLEKFGFETVFVPVDRYGRVDPEQLEAAIKDRTTLVSLILANNEVGTVQALAE
                     LVRVVRAHAGVAIHVDAVQAPAHMSLDVREMDVDLLCLASHKFEGPKGVGALYLRHGT
                     VLLPQVQGGAQERYRRAGTENVAGVVGMAHALELAVAERLEAGPRVARLRDRLLDALT
                     ALPNVELTGHPRKRLPGMLSLIVGGADGATIVSNLDLEGIECSTGAACTTGSTEPSHV
                     LTAMGYPEEEARGGLRLSLGRTTTEADIDRACAIIPGVIERIRATTSPIVPSATSLAA
                     RA"
     gene            complement(2053..3990)
                     /gene="typA"
                     /locus_tag="VMZ33_06615"
     CDS             complement(2053..3990)
                     /gene="typA"
                     /locus_tag="VMZ33_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HUR16939.1"
                     /translation="MTTSAQALTPASQKLATRDDLRNVAIVAHVDHGKTTLVDAMLRQ
                     TGAFRANQVVADRVMDSGDLEREKGITILAKQTGVQHGDVRLNIVDTPGHADFGGEVE
                     RSLLMVDSVLLLVDAAEGPLPQTRYVLQKAMARHLPVVVAINKIDRTDAQPAAVVDAV
                     YELFMDLGATDRQIEFPIVYTNAKLGTATLDLGKQGTDLRPLLDLLVDHTPAPTYEPG
                     HPLQLLVTNLSANDYVGRMAVGRLWNGTICMGQRITVVREEADTTDGNVEPGRTVTMT
                     GTVTSLTTARGIEREDISEAGPGEIVAVAGLPEVTIGDTITDPQDPRPLPRLDVDEPT
                     LSMTIGVNTSPLSGRDGKLLTSRQIKARLEREVLGNVSIEVRPTESPETFEVRGRGEL
                     QLAVLIEQMRREGFEMQVSRPEVLLREIGGETHEPYERVTIDIPPDYIGPVSQSLAAR
                     KGRMEQMTTDADGRVRMEFIMPVRGLVGYRGQLLTETRGTGLMHQIGEGYGPWAGEVT
                     HRTTGVLVSDRTGTSNAYGLFNLQERAAMLIGAGEEVYEGMIVGENNRPNDMDVNPTK
                     EKKLTNIRTHSHDEGLRLTPPPPMTLESAIEFIAEDELVEVTPTNLRLRKRALSQHDR
                     RREAGREYDERRRAEKAADQP"
     gene            4046..4756
                     /locus_tag="VMZ33_06620"
     CDS             4046..4756
                     /locus_tag="VMZ33_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HUR16940.1"
                     /translation="MTVAAVILHARVEGALADAAGRSAARRVVESAWAGGATPIVVVS
                     FDSAAGELAASLAGSPAVLAEPAPVEVGAVGQMLRGITVAAATVTETEAVLIWPGRLT
                     WVDAETVTSLIEAFGSRRDDLLRPTYEGQAGWPVLVPIAAVPAMQALDRSLMPDAVIE
                     ALARVAPAVWTVDTGDPGVIHDIAEHLDSLPAYQGPPEPVAGRAPEWGSPAADIADDS
                     PLSGPALAPYAQAAQEGD"
     gene            5290..5994
                     /locus_tag="VMZ33_06625"
     CDS             5290..5994
                     /locus_tag="VMZ33_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR16941.1"
                     /translation="MTAEDERLGHLLKLLRQRSGRTQVQLALAAAVPLRDLKRVEDGL
                     AAVIKLGRLRDLLAACDGRGRFVPWWNGASADRLLDERHARLVERVVAVLAARGWEVD
                     VEMSFAEYGERGSIDVFASRRDRSAVAVCEVKSALGSLEETNRVLDVKERLAPKIAYK
                     RLGWRPSVIGRLLILPRNRTVQRVVAEHASTMLAIYPARGREVRAWLREPTMSVRGIW
                     FVSDGPNATAISPPSV"
     gene            complement(6103..>6793)
                     /locus_tag="VMZ33_06630"
     CDS             complement(6103..>6793)
                     /locus_tag="VMZ33_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HUR16942.1"
                     /translation="VEAAGASPGDLVLIVADQDIVAQEVLGELRAELGGRLGLVADPN
                     ELSYLWVYDFPMYKWDSDNQRWDATHNPFSGVRPEDEPLLTTASGDIYAAPSEADPAG
                     TARALQHDLVLNGWELGGGSIRIHRHDLLERSFLLQGHSVEAQRAKFGAVLDAFEYGP
                     PPHGGIALGIDRWIALLTSQENIREVQAFPKTGSGSDLMLGAPSPVDPAQLAELALML
                     APPKTKEPAKA"
BASE COUNT         1024 a         2229 c         2301 g         1239 t
ORIGIN      
        1 gggtgacgat gttggtggca gcgtcgatgg tgttgacgaa acgcggctcg cccagcgcga
       61 cgccgagccc ctgccgttgc ccgatcgtgt acgcggccgc tccgcgatgg tcaccgacgc
      121 gcgtcccatc gacgtcgatc aggggccccg cctcctcgtg ccatccagct cgctccttca
      181 gcgcagtccg gtaatcgcca ccgggaacga agcagatctc ctgactctcc ggcttctcgg
      241 cggtggccag cccatgtcgt cgagcgacgt cgcgcacctc agccttggtc atcgctccga
      301 gtgggaagcg gacgtggccg agctgctcct gccgcaggcc gtacaggaag tagctctggt
      361 ccttgtctgt gtccagacct ttgagcaggc ggtagcggcg gccgccgggc gcgtccggat
      421 cctcgtgcgc atcgacccgc gcgtagtggc cggtggcaac ggcggtgcag tcgtagtggc
      481 gcagcgcctt gcccaggagc gcgccgaact tcacgtaact gttgcagtca acgcacgggc
      541 tgggcgtacg cccatcgagg tacgcgtcaa gaaaaggctg gaggacgccg gcgccgaact
      601 cgcgctccaa gttgaggacg taaaacggga tgcccagctg gccggccacg cgccgcgcgt
      661 cgtcggccgc atccagggag cagcagctct tcttgaactc gctgtaagcg tcggcgacgt
      721 cgtggaggcg catccagacg cccaccgtct cgaccccctc ctctgccagc aaagccgcgg
      781 cgaccgatga atcgacaccg cccgacatgg ccacgagcac tcgctcacgc atggggagcc
      841 ctcgatcagg cacgggccgc aaggctcgtg gcactgggca cgatggggct ggttgtggcg
      901 cggatgcgct cgatgacgcc ggggatgatg gcgcacgctc ggtcgatgtc cgcttcagtc
      961 gtcgtgcggc cgagggacag ccgcagaccg ccccgtgcct cctcttccgg gtagcccatg
     1021 gcggtcagga catgtgacgg ctcagtcgag ccggtcgtgc acgccgcgcc ggtcgagcac
     1081 tcaatgccct ccagatcgag gttggagacg atcgtggcgc catccgcgcc accgacgatg
     1141 agcgagagca tgcccggcag gcgcttgcgc gggtggcccg tgagctcgac gttcggcagc
     1201 gccgtgagcg cgtcaaggag ccggtcgcgc aggcgtgcca cgcgtggccc cgcctccagc
     1261 cgttcggcca cggcaagctc caaggcgtgt gccatgccaa cgaccccggc cacgttctcc
     1321 gtaccggccc gacggtagcg ctcctgcgcg ccaccctgca cctgcggcag cagtaccgtc
     1381 ccgtgcctca ggtagagcgc tccgacaccc ttcggcccct cgaacttgtg gctggcgagg
     1441 cacaacaggt ccacgtccat ctcacgcacg tccagcgaca tgtgtgccgg tgcctgcacc
     1501 gcgtcgacgt ggatagccac acctgcgtgg gcgcggacga cgcgcaccag ctcggccagc
     1561 gcctgaaccg tcccaacctc gttattggca aggatcagcg atacgagagt cgtgcggtcc
     1621 ttgatcgccg cctcgagctg ctccggatcc acgcgcccgt agcggtcgac cggcacgaac
     1681 accgtctcga aaccgaactt ctcgaggtgg cgcagtgcgt acatcgttgc gtggtgctcc
     1741 accgcggagg tcacaagtcg gttgccgcgc gccttgccgg cccacgcaat gcccttcagc
     1801 gcaaggttga tcgactccgt gccgccaccg gtgaagatga tctcgcgcgg ctcggcgttg
     1861 agtgccgccg cggtccgctc gcgagcctcg tccagggccg cccgcgcgcg gcggccgatc
     1921 gcgtggatcg acgagggatt gccgaaccca tcggtcagcc acggcatcat ggcgtcgagg
     1981 gcttctcgac gcagtggcgt cgtcgcggcg tggtcgaggt agaccggcat cggccgattc
     2041 tacgtccgcc ctctaaggct ggtcggctgc cttctcggca cgacgccgct cgtcgtattc
     2101 tcggcccgcc tcgcgccgtc ggtcgtgctg gctcaaggcc cgctttcgga ggcggagatt
     2161 ggtcggggtg acctcgacca gctcatcctc ggcgatgaac tcgatcgccg actcgagggt
     2221 catcggtggc ggcggggtca ggcgcaaacc ctcgtcgtgg ctgtgcgttc ggatgttggt
     2281 cagcttcttt tccttggtcg ggttgacgtc catgtcgttc ggccggttgt tctcgccgac
     2341 gatcatgccc tcgtagacct cctcgccggc gccgatgagc atcgccgcac gttcctggag
     2401 gttgaagagt ccgtaagcat ttgacgtgcc agtgcggtcc gagacaagca ctccggtcgt
     2461 gcggtgcgtc acctcccccg cccacggtcc gtagccctcg ccaatctggt gcatcagccc
     2521 ggtgccgcgc gtctctgtca gcagctgtcc ccggtagccc acgagcccgc ggaccggcat
     2581 gatgaactcc atgcgcactc ggccatcggc atcggtggtc atctgctcca tccgaccctt
     2641 gcgcgctgcg agcgactggc tgaccggccc gatgtagtca ggcgggatgt cgatcgtcac
     2701 ccgctcgtac ggctcgtggg tctcgccgcc gatctcacgt aggagcacct ccggtcgcga
     2761 aacctgcatc tcgaagccct cgcggcgcat ctgctcgatc agcaccgcca gttgaagctc
     2821 gccgcgtccg cgtacctcga aagtttccgg cgactcggtc ggccgcacct cgatcgagac
     2881 gttgcccagg acctcgcgtt cgaggcgtgc cttgatctgg cgcgatgtga gcaatttgcc
     2941 gtcgcgaccc gaaagtggcg aagtgttcac gccgatcgtc atcgacagcg tcggctcatc
     3001 aacgtccagt cgcggcagcg gtcgcggatc ctgcgggtcc gtgatggtgt ccccgatcgt
     3061 gacctcaggc agcccggcga ccgccacgat ctcaccggga ccagcctcgc tgatgtcttc
     3121 gcgctcgatg cctcgcgccg tcgtcaggct ggtcacagtc cctgtcatcg tgacggtgcg
     3181 gccaggctcc acgttgccgt cggtcgtgtc ggcctcttcg cggacgacgg tgatgcgctg
     3241 acccatgcag atcgtgccgt tccagaggcg gccaacggcc atgcggccga cgtaatcgtt
     3301 ggcagacagg ttggtgacca gaagttggag ggggtggcca ggctcgtagg tcggcgccgg
     3361 cgtgtggtcg accagcaagt cgagcagcgg gcgcaagtcg gtgccctgct taccgagatc
     3421 cagcgttgcg gtacccagct tggcattcgt gtagacgatc gggaactcga tctggcggtc
     3481 ggtcgcgccg aggtccatga acagctcgta gacggcatcg acaaccgcgg ccggctgtgc
     3541 atccgtgcgg tcgattttgt tgatcgccac gaccaccggg aggtggcgcg ccatcgcctt
     3601 ctgcagcacg tagcgcgtct gcggcagcgg tccctcggcg gcgtcgacga gcaggaggac
     3661 cgagtcgacc atgagcaggc tgcgttcgac ctcgccgccg aagtcggcgt ggcccggggt
     3721 gtcgacgatg ttcaggcgga cgtcgccgtg ctgcacgcca gtttgcttgg caagaatcgt
     3781 gatgcccttc tcgcgctcga ggtcgcccga gtccatgact cggtcggcca ccacctgatt
     3841 ggcgcggaag gcgccggtct gacggagcat cgcgtcgacg agcgtggtct tgccgtggtc
     3901 aacgtgagcg acgatcgcga cgttgcgcaa gtcgtcgcgc gtggcgagtt tctgagaggc
     3961 aggcgtcaac gcctgtgccg aggttgtcat gggagcttcg attgtcccac gaagcccgct
     4021 tcggtgggct cgttagcatc tgcaggtgac tgttgctgcc gtgatcctgc atgcccgcgt
     4081 tgagggcgcc ctggccgacg ccgctggccg ctcagccgcg cgacgggttg tggagtctgc
     4141 ctgggcgggg ggcgcgacgc cgatcgtggt ggtgtccttc gactcagccg caggtgagct
     4201 ggctgcgtcg ctggcaggtt cgcctgcggt tctggccgag ccagcgccgg tggaggtcgg
     4261 agctgtcggc cagatgttgc gtggcattac cgtggctgcc gccactgtca cggagacgga
     4321 ggccgtcctg atctggcccg gccggctgac gtgggtcgat gccgaaaccg tcacttcttt
     4381 gatcgaggcg ttcggctcac ggcgcgatga tctcctgcgg ccgacgtacg aaggtcaggc
     4441 gggctggccc gtcctggtgc caattgctgc ggtccccgcg atgcaggcac tcgaccgctc
     4501 gttgatgccc gacgcggtca ttgaagctct ggcgcgggta gcgccggccg tatggacggt
     4561 cgacaccggg gatcccggcg tgatccacga catcgcggag catctcgatt cactgcccgc
     4621 ctaccagggc ccgcccgagc cagtcgccgg tcgtgcaccg gagtggggct ctccggccgc
     4681 cgatatagcc gacgattcgc ccctcagcgg cccggctctg gcgccgtacg cacaggccgc
     4741 ccaagaaggc gactagacgt gcgcgtagag aggccgcagc gtcctcagat tcgtcatctg
     4801 gcggctttgg ctgcgttcgc gtggatcgcc ctaggagttc tcttcagcta tggattgatc
     4861 tacattggct ggggcgcgcg tctccacgaa atcactttcc tagtggtggt gatagcggct
     4921 agttttggcc tcgggattct tctcatgggt cgcctttcgc gtcggcgatt ggcagcggct
     4981 gcgtccatag gcgcatctct cgctgtcgct gtcggcctct ggtggatcct tgtctcgaac
     5041 cgcggtgtcc gcgtcgagga caccatggca attggggtct tcgggattgc cgcgttgctg
     5101 ccagctgtgg cttggtttcg gtggcgggta gcagaggatt agtaactagg acgctgacgg
     5161 agtcgacgta actgggctag cgaacttgcg gtgcggccca atggcgcaag gcacgctcgc
     5221 gtactgtttt gcacgctggt aacggacaga taagccggcg gtaagcgcgg ggcgccatcc
     5281 ttctgggccg tgactgcgga ggatgagcgg ctcgggcacc tgctgaagct gctgcgtcaa
     5341 cgctcggggc gtacccaggt tcaactcgcg cttgcggcag ccgtcccgct gcgtgacctg
     5401 aagcgcgtcg aagacggctt ggcagccgta atcaaactcg gccggcttcg ggatctgctt
     5461 gcggcatgcg atggtcgtgg ccgtttcgtc ccgtggtgga acggtgcgtc tgcggatcga
     5521 ctgctggacg agagacacgc ccggctcgtc gagcgcgtcg ttgcggtgct cgcggcgcgg
     5581 ggctgggaag tagacgttga aatgtcgttt gccgaatacg gcgaacgcgg ctctatcgac
     5641 gtcttcgcgt ctcgacgcga tcgctcggcc gtagcggttt gcgaggtcaa gagcgcgctc
     5701 ggttctctgg aagagacgaa ccgtgtcctg gacgtcaaag agcgcctcgc gccgaagatt
     5761 gcgtacaaac gtctcggatg gcgtccaagc gtgatcggtc gccttttgat cctgccgcgc
     5821 aatcgcaccg ttcagcgagt cgtcgcagag cacgcttcga cgatgctggc gatctatcca
     5881 gcgcgcggtc gcgaggtcag ggcttggctg cgcgaaccga caatgtccgt ccgcgggatc
     5941 tggtttgtgt cagatggacc aaatgcgacc gcgatctcgc cgccgagcgt ctaggttggg
     6001 tcaggtggac cgctgagggc cggctgacgc aaagtgcgtg ggttcatcgg aaatgctggc
     6061 tcagatgcgt ccaaatgacg caagacgccc ggcccgggga gtctaggcct ttgcaggttc
     6121 cttcgtcttc ggtggggcga gcatcagcgc caactcggcg agctgcgcgg gatcgacggg
     6181 cgacggcgcg ccgagcatca ggtctgagcc gcttcccgtc ttcgggaacg cctgcacctc
     6241 gcggatgttc tcctgcgacg tgaggagggc gatccaacgg tcgatcccca gcgcgatgcc
     6301 accgtgcggc ggcggcccgt actcaaaggc gtcgagcacc gcaccgaact tggcacgctg
     6361 agcctcgacc gagtgaccct gcagcaggaa cgaccgctcg agcaggtcgt ggcgatggat
     6421 gcggatggat cccccgccga gctcccaccc attcaagacg aggtcgtgct gcagcgcccg
     6481 tgcagttccc gccggatcag cctcggacgg tgccgcgtaa atgtcgcccg acgcagtcgt
     6541 caggagcggc tcgtcctccg gccggacgcc ggagaacggg ttgtgcgtgg cgtcccagcg
     6601 ctggttgtcg gagtcccatt tgtacatcgg gaagtcgtag acccagagat atgacagctc
     6661 gttcgggtcc gccacaagac cgaggcgccc gccaagctcc gcgcgcaact ccccgaggac
     6721 ttcctgagcg acgatgtcct ggtcggcgac gatcagcacc agatccccgg gcgacgcgcc
     6781 ggccgcctcc acg
//
LOCUS       DATLCO010000289         6709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4287729, whole genome shotgun sequence.
ACCESSION   DATLCO010000289 DATLCO010000000
VERSION     DATLCO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4287729"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VMZ33_06635"
     CDS             complement(<1..1153)
                     /locus_tag="VMZ33_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HUR16943.1"
                     /translation="MLLSRMAGGPSRMPWGIALPFILAAAAVITPASVAVSPARADSV
                     PPKAVFIVGPTHDQTDGNLEDAEVMAQQAEAAGMDVRRVFFPYATWDNVLANIQGASM
                     VVYMGHGYGWPSPYTTKLTEARQNGMGLNSFAGSGRAEYTYYGATPIKSNIHLATNAV
                     VFNVHLCYAAGNAESGMALPSDDLARERVDNFANGWLAAGAGAVFAFSWGSHLNLPNQ
                     LMTSNQTMDQMFNIAANGSPMGHVGWRDLYYDSLRTPGARNHLDPHPSYGFYRAVSGN
                     LNMTTAEFRAGALPDATPPPPPPGDPANPPQVTYLAPTVSQFHPNGDGLAEELVVNHV
                     VSRSSYLDVAITNQAGETVRTYSIWATGTGASRWNGKDNAGTLVPDGSYT"
     gene            complement(1418..2473)
                     /locus_tag="VMZ33_06640"
     CDS             complement(1418..2473)
                     /locus_tag="VMZ33_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16944.1"
                     /translation="MGAEIGMTRVLLTCAGGAYGLGVSRSLRASSNPYHVIATDADRF
                     SFQRAEGDERRRVPRASDPRFLPTVRELVRSTGADFLWPGHDSDIRAFAKDRASLGAA
                     TFLPQLEEIDICRNKMLSYGRWKERGVRVPETVLVGDREGLAAAFDLFGGEIWLRTVT
                     GAGGTGALGTDSLSKAEAWLEVHDGWGRFTAARWIKGGQRLSWESVWANGQLIAVQGR
                     RQLVQGFEYLTLSRITGVPGVNQWGTPAEADETGMAAVRAISPSPHGNFGVDMVCDAD
                     GTPYVTEINIGRFNNDGLIHWPDAKLNAADLAVRLGLGEKPPFDPPLMHPKKRDSVII
                     YGIPALPVEVPWEEVRE"
     gene            2535..3632
                     /locus_tag="VMZ33_06645"
     CDS             2535..3632
                     /locus_tag="VMZ33_06645"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HUR16945.1"
                     /translation="MIAADVIRQAPKVLLHDHLDGGLRPQTVIELAREVGYGGLPTTD
                     ADELALAFRAGADRKSLVLYLEGFDHTVGVMQTRDAIIRVAAECAEDLAADGVVYAEV
                     RYAPELSTRGDLTLDEVVLAVLEGFERGSSGRGITMKFIATGMRQFARSVEIAELALR
                     HRDAGVVGFDVAGPEAGFPPTRYLDAFNIIHQGNFHLTIHAGEAFGLPSLWEALQWCG
                     AERLGHGVRIVDDITVDPDGEVHLGRLAAYVRDTRVALEMCPTSNVHTGATSSIEEHP
                     IDLLRRLRFRVTVNTDNRLMSGVSMSSEFETLSRTFGFGLDEMQWLTLNAMKSAFAPF
                     DERLRLINGVIKPTYARLMAETAFAETAARY"
     gene            3660..4373
                     /locus_tag="VMZ33_06650"
     CDS             3660..4373
                     /locus_tag="VMZ33_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16946.1"
                     /translation="MDEEHEKSVEHETSDRGTLAGMASAVVDAAAPVISAMGTLAAGA
                     RRILDERPGARVRRVRRMGRQPLANLWDLHPEAYRATPRDFGFHAVAVESIAGTAVEG
                     APQRGGDFLPLKDRRSEDWKSRWQRIRAAIDALANLPPIELVKYDDSYWVLDGHNRVA
                     AALYNGQPEIDAVVRELRRPGHTSRGPTPLIAGVVEDSIDLRNAGTGRRTRTTKHATN
                     LISKPHEHAHERPDDGDDR"
     gene            4430..5074
                     /locus_tag="VMZ33_06655"
     CDS             4430..5074
                     /locus_tag="VMZ33_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUR16947.1"
                     /translation="MRLLAVSDEREPAFDNEVNRRALGAIDGLIGAGDLEPGYLSFLA
                     DAFHVPLFHVRGNHDRGGGWEETSDKLPEPMDGSWHDLAGLAVAGLSWPLSKHVHAIH
                     DDGAAWRQAAAGYVRLRRRRPEVIVSHVPPLGIGDAGEDDLYHRGFAAYRWLLKRLKP
                     MLWIHGHTTPASVREWSLRADGTTVVNVTGAVLIDMVPEAIRQADDSRVGSTIA"
     gene            5075..6289
                     /locus_tag="VMZ33_06660"
     CDS             5075..6289
                     /locus_tag="VMZ33_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HUR16948.1"
                     /translation="MTRILLYTGKGGVGKTSIAAATAVLSARRGIRTLIVSTDIAHSL
                     ADALDTPVGPEPVQIEPNLWAHEPDVFFNISKYWRTIQSYFASLFSWGGLDEVMAEEM
                     TVLPGMDELGNLLWIGDHVDQGAYDLIVVDAAPTGETVRLLSLPEASRWWIEKIAPIG
                     RRVQKLGGPLLRRIVGVPLPSDDVYVAGEELLTRLKYVHDLLANPDRSSVRLVLNLEK
                     VSIQEAQRSFTYFHLYGFPTDLIVANRVLPSASGAYFDAWREAQARYLPLVEEQFAPV
                     PVRTVPFFDKEMVGIERLAELGTALFGDEDPSQFRYRGRPYSVSREDGGYTLSVELPF
                     ASKEEISLSRATDELVLQVGTWRRNLVLPRLLIDAPTLGAKMEDRVLKVRFAQPRGTS
                     GGSRDGSGSKHG"
     gene            6282..6569
                     /locus_tag="VMZ33_06665"
     CDS             6282..6569
                     /locus_tag="VMZ33_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16949.1"
                     /translation="MDDERIQRLEARLEELDRRNTSLERAMDKAMDRSRAAMDTIVPN
                     DTRRHMRAAWRENLLAVRSLIDFWADRLADDRSGSDSANTSNGQRETIKID"
BASE COUNT         1096 a         2191 c         2238 g         1184 t
ORIGIN      
        1 tcgtgtagct gccgtcgggg acgagcgtgc cggcattgtc cttgccgttc cagcgcgacg
       61 cgcccgtgcc agtcgcccag atggagtagg tccggaccgt ctcgccggcc tggttggtga
      121 tggccacgtc gaggtagctc gaccttgaga cgacgtggtt caccaccagt tcctcggcga
      181 gcccgtcgcc gttagggtgg aattggctga cggtcggcgc caggtaggtc acctgtggcg
      241 gattggccgg atcgcccggt ggcggtgggg gaggcgtggc atccggcagc gcgccggctc
      301 ggaactcggc cgtcgtcatg ttcaagttgc cgctgaccgc gcggtagaag ccgtagctcg
      361 ggtgcggatc caggtggtta cgcgcccccg gtgtgcgcag ggagtcgtag tagagatcgc
      421 gccagccgac gtggcccatc ggcgagccgt tggcggcgat gttgaacatc tggtccatcg
      481 tctggttgga cgtcatgagc tggttgggga ggttcaggtg tgagccccag ctgaaggcga
      541 acactgcgcc ggcgccggcg gccagccagc cattggcaaa gttgtcgacc cgttcgcgcg
      601 cgaggtcgtc cgaggggaga gccatgcccg actcggcgtt gccagcggcg tagcacaggt
      661 ggacgttgaa gaccacggcg ttggtcgcca ggtggatgtt gctcttgatt ggcgtcgcac
      721 cgtagtaggt gtattcagcg cgaccgctgc cggcaaagct gttgagcccc atgccgttct
      781 ggcgcgcctc agtcaactta gtggtgtagg gactcggcca cccgtagccg tggcccatgt
      841 acacgaccat gctcgcgccc tgaatgttcg ccagcacgtt gtcccaggtc gcgtagggga
      901 aaaacacgcg ccggacgtcc atgcccgccg cctctgcctg ctgggccata acctcagcat
      961 cctcgagatt cccgtcggtc tggtcgtgcg tcggtcctac gatgaacacg gcctttggcg
     1021 gtacggaatc agcccgcgcc ggtgaaacgg caactgatgc gggcgtgatg accgcggcag
     1081 cggcgaggat gaacgggagc gcgatgcccc aaggcatccg gctgggcccc ccggccatcc
     1141 ggcttagcag caatccaggt cctccctctt tcacacccgc aacacggctg ttcggtgtga
     1201 acgtcgaaac ggcaccacgg cggcgtgccg tcttcactat ctgcgcggac ggcctggggc
     1261 cgctgctacg cgatctcttg acggggcgct tgttatgtcg ttgaaacgac gtcgtcagta
     1321 tggcgtgcat agccaacgtg tgcatcatgc atcggggcta ttcgcgggca cttatcgggc
     1381 ccttcccggc ggcgtacctc atcgtccaca cgacggtcta ctcgcgcact tcctcccacg
     1441 ggacctcaac cggcagcgcg gggatgccgt agatgatcac gctgtcgcgc ttcttcgggt
     1501 gcatcagcgg cgggtcgaac ggcggcttct cgccaaggcc caggcggacc gcgaggtcgg
     1561 cggcgttcag ttttgcgtca ggccaatgga tcaggccgtc gttgttgaag cggccgatat
     1621 ttatctccgt gacatagggc gtcccgtcgg catcgcacac catgtcgacg ccgaagtttc
     1681 cgtgcggcga gggcgagatc gcgcggaccg ccgccatgcc tgtctcgtcg gcctccgccg
     1741 gcgttcccca ctggttgacg cccggcaccc cggttatgcg tgacaacgtc aggtattcga
     1801 agccctgcac aagctggcgc cggccttgca ccgcgatgag ctggccattc gcccaaaccg
     1861 actcccacga cagccgctga ccgcctttta tccagcgcgc cgccgtgaat cggccccagc
     1921 cgtcgtggac ctccagccac gcttcggcct tcgacaagct gtcggtgccc agcgcgcccg
     1981 tgccacccgc gccggtcacg gtcctcagcc aaatctcgcc accgaacagg tcgaatgctg
     2041 ccgccagtcc ctcgcggtca cccaccagca cggtctcggg cactcgcacg ccgcgttctt
     2101 tccagcgccc gtaggacagc atcttgttgc ggcagatatc gatctcttcg agttgcggca
     2161 ggaaggtagc cgcgccgagg cttgcccgat cctttgcgaa cgcccggatg tccgagtcgt
     2221 ggcccggcca caggaagtcg gcgccggtgg agcgcaccag ttccctgacc gtgggcagga
     2281 acctcgggtc gctggcacgc gggaccctgc gccgctcgtc gccttccgca cgctggaatg
     2341 agaagcgatc ggcgtcggtg gcaatcacgt ggtaggggtt gctcgaggcc ctcagcgacc
     2401 gcgacacacc gagtccgtag gcgccgccag cgcacgtgag aaggacgcgc gtcatgccta
     2461 tttcggctcc catctcatgc ccaggttcca ccttgtatgt gcgcacgcaa tcgacccatc
     2521 tatgcttgaa ggacatgatc gccgctgacg tcatccggca ggcgcccaag gtgctgctgc
     2581 acgaccacct cgacggtggc ctccggccgc agacagtgat cgagctcgcg cgcgaggtgg
     2641 gctacggcgg cttgccgacg actgatgcgg acgagcttgc gctggccttc cgggcgggcg
     2701 ccgaccgcaa gagcctcgtc ctctatctgg aggggttcga tcacaccgtc ggggtgatgc
     2761 agacccgtga cgcgatcatc cgcgtcgccg cggagtgcgc cgaagacctc gcggccgacg
     2821 gtgtcgtcta cgccgaggtc cgctacgcgc cggagctctc gacgcgtggg gatttgacgc
     2881 tcgacgaggt ggttctggcc gttctcgagg ggttcgagcg cgggtcgtct ggccggggca
     2941 tcacgatgaa gttcatcgct accggcatgc gccagttcgc gcgctcggtg gagattgccg
     3001 agctcgcgtt gcgacaccgc gatgctggcg tggttggttt cgatgttgcg ggcccggagg
     3061 cgggcttccc acccacccgc tacctcgacg cgttcaacat cattcaccag ggcaacttcc
     3121 acctgacgat ccacgctggc gaggccttcg ggctgccgtc cctctgggag gcgctccaat
     3181 ggtgcggcgc agagcggctt ggccatgggg tgcggatcgt cgacgacatc acggtcgatc
     3241 cggatggcga ggtgcatctc ggccgcctcg cggcgtacgt gcgcgatacg cgcgtggcgc
     3301 tcgagatgtg cccgacatcg aacgtgcaca ccggcgcgac gtcttcgatc gaagagcatc
     3361 cgatcgacct cctccgccgc cttcgcttca gggtcaccgt gaacaccgac aaccgattga
     3421 tgagcggcgt gtcgatgagc tccgagttcg agacgctgtc gcggacgttc ggcttcgggc
     3481 tggacgagat gcagtggctg acgctgaacg cgatgaagag cgcctttgca ccgttcgacg
     3541 agcgcctgcg gctgatcaac ggggtcatca aacctactta cgcgcgtctg atggctgaaa
     3601 cggctttcgc cgaaacagcg gcccggtatt agaggggtcg tggcgtacgc tcgttcctga
     3661 tggacgagga gcacgagaag tcggtcgaac acgaaaccag tgaccgcggg acgctggccg
     3721 gcatggcctc ggccgtggtc gatgccgctg cgcccgtgat ctcggcgatg ggcactctcg
     3781 ctgccggggc ccgtcgcatc ctcgatgagc gtcccggcgc ccgcgtgcga cgagttcgtc
     3841 ggatgggccg ccagcctctg gcgaacctgt gggacctgca tcccgaggct taccgcgcga
     3901 cgccacggga tttcggcttt cacgcggtcg ccgtggagtc catagccggg acggcggtcg
     3961 agggcgcccc gcagcgcggc ggcgatttcc tgccgctcaa ggatcgacgt agcgaggact
     4021 ggaagtcacg ctggcaacgg atccgcgccg ccatcgacgc gctggcgaac cttcctccga
     4081 tcgagctggt gaagtacgac gacagctact gggtgctcga cgggcacaac cgtgtagccg
     4141 ctgcgctcta caacggccag cccgagatcg acgcagtggt gcgcgaacta cggcgacccg
     4201 gccacacgtc gcgcggcccc acgccgttga ttgcgggtgt cgtcgaggac agcatcgatc
     4261 ttcgaaatgc gggcacgggc cgtcggacca ggaccacgaa gcatgcgacc aacctgatct
     4321 cgaagccaca cgagcacgcc cacgagcgcc ccgacgacgg cgacgaccga tgatcaggcg
     4381 attgacggtc tctcgtgcgc tgaccaaagc agcgggagac gctcgaacgg tgcgactcct
     4441 tgccgtttcg gacgagcgtg agccagcctt tgacaacgaa gtcaaccggc gcgcgctggg
     4501 ggcgattgac gggctgatcg gcgcgggtga tctcgagccg ggttacctgt cgttccttgc
     4561 cgacgccttc cacgtgcccc tgttccatgt ccgcggcaac catgaccgcg gcggtggctg
     4621 ggaggagacc agcgacaagc ttcccgagcc gatggacggg tcctggcacg atctggctgg
     4681 tctggctgtg gccggacttt cctggccact ctcgaagcac gtccatgcca ttcacgacga
     4741 cggcgccgcg tggcggcaag ccgccgccgg ctacgtgcgc ctgcgacgtc gccggccgga
     4801 ggtcatcgtc agccacgtgc cgccgctggg tatcggcgac gccggcgagg acgacctgta
     4861 tcaccgtggc ttcgccgcgt atcgctggct gctcaagcga ctcaagccga tgctttggat
     4921 ccacggccac accacgccgg cgtctgtcag ggagtggtcc cttcgggccg atgggacgac
     4981 ggtggtcaac gtcaccggcg ccgtgctcat cgacatggtt ccagaggcca ttcggcaggc
     5041 ggatgactca cgagtcgggt ctacgatcgc ctaagtgact cggatactcc tgtatacggg
     5101 caagggcggc gtcggcaaga cgagcatcgc cgctgcaacg gcggtgctct cagcgcgacg
     5161 cggtatccgg acgctcatcg tctcgacgga catcgcccac agtctcgccg acgcactcga
     5221 cacgcccgtc gggccagaac cggttcagat cgagccgaac ctgtgggccc acgagcccga
     5281 tgtcttcttc aacatcagca agtactggcg cacgatccag tcctatttcg cgagcctgtt
     5341 ctcgtggggt ggcctggacg aggttatggc ggaggagatg acggtcctgc cgggcatgga
     5401 tgagctcggc aacctgctgt ggatcggcga ccacgtggat cagggcgcgt atgacctgat
     5461 cgttgtcgac gccgcgccga cgggcgagac ggtgcggctg ctcagcctgc ccgaggcgag
     5521 ccgctggtgg atcgagaaga tcgcaccgat cggccggcgg gtgcagaagc ttggtggccc
     5581 cctgctgcgg cggatcgtgg gcgtgccgct gccgtcggac gacgtgtatg tcgccgggga
     5641 ggagctgctc acgcggctca agtacgtcca cgaccttctg gccaatccgg atcggtcgtc
     5701 cgtgcgactg gtcctgaacc tcgagaaggt ttcgattcag gaggcgcaac ggtcgttcac
     5761 ctacttccac ctgtacggct ttccgaccga cctcatcgtc gccaaccgcg ttctgccctc
     5821 ggcgagcggc gcctatttcg acgcctggcg cgaagcccag gcgcgctacc tgccgctggt
     5881 cgaggagcag ttcgccccgg tgcccgtgcg gaccgtgcca ttcttcgaca aggagatggt
     5941 gggcattgaa cgcctggccg agcttggcac ggcgctgttc ggtgacgagg acccgtcgca
     6001 attccgctat cgaggacgtc catattcggt cagccgtgaa gacggcggct acacgctgtc
     6061 ggtcgagctt cccttcgcgt cgaaggaaga gatctcgctg tcgcgtgcca cagatgagct
     6121 cgtgctgcag gtcggcacat ggcgccgtaa tctcgttttg ccgcgactgc tcatcgatgc
     6181 gccgacactc ggcgccaaga tggaagatcg cgtgctcaag gttcgcttcg cgcagccgcg
     6241 aggcacctct ggagggtcga gagatggatc aggatcgaaa catggatgac gagcgcattc
     6301 agagactcga agctcgtctc gaggagctcg accgacgcaa tacgtcgctc gagcgcgcca
     6361 tggacaaggc gatggatcgc tcccgcgcgg cgatggacac gatcgtgccc aacgacaccc
     6421 gccgccacat gcgcgcggcg tggcgcgaga acctcctcgc cgttcgcagc ctgatcgact
     6481 tctgggccga tcgactcgct gacgatcgga gcggctccga ctcagcgaac acgtccaatg
     6541 ggcagcgcga aaccatcaag atcgattgag catcgaggta cggctcattg agccagggga
     6601 gctgcgcgcc tacttcgact cggtaaacct gtccttcggg gagcacccgg cagaggagca
     6661 gtgggcgctc gaccagctga cccttgaggc ggaccgcgtc cacgccgct
//
LOCUS       DATLCO010000290         7404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4294085, whole genome shotgun sequence.
ACCESSION   DATLCO010000290 DATLCO010000000
VERSION     DATLCO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4294085"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1449)
                     /locus_tag="VMZ33_06670"
     CDS             complement(202..1449)
                     /locus_tag="VMZ33_06670"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HUR16950.1"
                     /translation="MTSALRQVDRNGQVFIVSAARTPIGKFGGSLADVPATTLGGIAI
                     KAAVERAGLPEGTPIDEVLMGQVLQAGVGQAPARQAALKAGLGEGTSATTINRVCGSG
                     LKAIMLAAAEIRAGDAEIVVAGGMENMNLGPYLIPKARFGYRLGNAEMIDATVHDGLW
                     CSTESCHMGTHAERVAIKNQVSREDQDQFALESHQRALAAIKDGRFAEEIVPVPVKKG
                     KEMVEFAVDEGPRPDSSLEALGKLKPVFALPDPDPDSTVGLPGGTVTAGNAPGITDGA
                     AATVVASERAVERLGLTPLARIVGYAQAEVEPKWLFLAPVKGVQKLLARIEMPIEAFD
                     LIEINEAFAAQVLADGRELGFDWSKVNVNGGAIALGHPIGASGARIVATLLHELRRRG
                     GRYGLATLCLGGGGSVAMAVERV"
     gene            1767..2285
                     /locus_tag="VMZ33_06675"
     CDS             1767..2285
                     /locus_tag="VMZ33_06675"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUR16951.1"
                     /translation="MTQAELQIINGVDVGELNEYIESCRLDPARADRNPVVVARWVGG
                     GRAEVTSSLGGAPVYMGGPRDPSALGMLLRSLAACDVEIVATTAARLGLEIEDLSVEA
                     RGFANVGRYLGLDSALSPGYQNIAYSIRLKTKGDATPEQLDAIRRACEEGSPVGDTLK
                     RPIPLVLEFDAN"
     gene            2304..3191
                     /locus_tag="VMZ33_06680"
     CDS             2304..3191
                     /locus_tag="VMZ33_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUR16952.1"
                     /translation="MARLQRRRFSESSDVRSFPHGKVEVVELDDRAVGRLTYEPGWRW
                     SVDVRPIASTQSCQFHHVGVTVSGRLRVLMQDGVELEIGAGDVFEIPPGHDAWVVGDE
                     PWISIDFEAMRSFGRATGDNSRRVLSTIVITDIVDSTTRAHAVGPARWREIVSQHNEI
                     SERIIDAYHGRLVKTTGDGVIGLFDSSERAIRAALQLSDAIAALDIAIRAAVHSGEIE
                     IQANDIRGVAVHTAARMMTVAGPGDVIVSSTVRELLDDSDVALEDFGTHQLKGLPGQR
                     QLFRVVARRGRSVPPAAAH"
     gene            3262..5313
                     /locus_tag="VMZ33_06685"
     CDS             3262..5313
                     /locus_tag="VMZ33_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR16953.1"
                     /translation="MPQQTLTEAQLRERGLAGLAELARLDERTGSINAVAARALDLVG
                     SLLDADRAAVCAIDGDETIEWLAHRRMERFIAASADLRPSDLLWARRPLETGRPELFD
                     RRAPSHERSALSDTADALGMAAFAIVPLRSGQEFTGILGLVWNDDPPELGADEELLTT
                     IGRLVALALGNTRLRDTLLARQSELDESEARYRSLFEQAPEPMLICDWDGAVLDFNRA
                     AMALYGVTGHRLLNHRLETLTDISLDRRRRLAAALHRSRRAEVEARGRRRDGSEFPMV
                     LHLAVIAFRGEERLLVQLRDMTDQQRMQAELLQAQKMEALGQLISGVAHELNNPLSAI
                     IAFSQLLRSDERLPADLARDADLLVGEADRTRRIVQNLLDFARQRTPDRQPTSLPALV
                     ERTLELHAYALRAGRIETRVLMPTDLPDVWADSAQIQQVLLNLTLNAIQSIRASNPNG
                     TLTIAAHTEPRSDGPRFVRLRVEDDGPGVELQSRDHLFEPFFTTKDVGQGTGLGLSVS
                     YGIVAAHGGKLWYEPASVAGATFLLELPAAADIERTLAGKSERPATAAANRGLGSMVL
                     VVDDERAVRETIRRALERAGHSVTVAGSGAEALEILRDRSVDLLLIDHRMSAMDGTEF
                     YGYAIAARPELTGRALMMTGDTLNPELLEFVRENSLSLIAKPFDIDDLTAEVDSALAP
                     A"
     gene            complement(5320..6198)
                     /locus_tag="VMZ33_06690"
     CDS             complement(5320..6198)
                     /locus_tag="VMZ33_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HUR16954.1"
                     /translation="MSERKVLVCGAGLMGHGIAQVMAAAGHRVWLYEPEAARAEAGRE
                     RIAGNLERAVAKGKISDEDRTAQLGRVSVAASLAEAASQAELVVEAIFEDEAVKQSLF
                     AEIDAAAPPSSVLASNTSSISITRLAAATGAGRRARFVGMHFFSPVPVMPLIELIRGA
                     ETDDATEAYVRDVAAELGKQVIVSKDRPGFIVNRILMPYLGESMRAFEEGLGTAEDID
                     SGAKVGLNHPMGPLELADFIGLDVCLHIMEVLQDGFGAAHMAPPPILRQLVAAGHLGQ
                     KTGRGFYTYPREPKKG"
     gene            complement(6220..7215)
                     /gene="meaB"
                     /locus_tag="VMZ33_06695"
     CDS             complement(6220..7215)
                     /gene="meaB"
                     /locus_tag="VMZ33_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HUR16955.1"
                     /translation="MTASADRSLAERARGGDRRALARLLTHVENRTAAGEDALRELYP
                     HAGSAHLVGVTGAPGSGKSTLVAALVGEARKSGRPVAVIAIDPSSPITGGAILGDRVR
                     MQEHASDTDVFIRSMASRGHSGGLAAATIAAAAAFDASGFGLIFIETVGTGQSEVEIA
                     AIADTTLVVQAPEMGDEVQAIKAGLLEVADIVVVNKSDRPGADRAAAQLRGMLTVGAQ
                     HDRAMGDRPRPKRPDVLLASAMTGAGVPELLAALDHRAAARRAADSDPSVALAAAQKR
                     AEAQLSGIMADRLRTRLATAAHRAAAADVVTRVARHEQDPYSAADELLAKLADNS"
     gene            complement(7212..>7404)
                     /locus_tag="VMZ33_06700"
     CDS             complement(7212..>7404)
                     /locus_tag="VMZ33_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16956.1"
                     /translation="LPKITAALREQGMDDVLVTAGGIIPDADIPQLREVGVARVFGPG
                     TTIREVAEFFRSNVARREG"
BASE COUNT         1216 a         2498 c         2476 g         1214 t
ORIGIN      
        1 cgcagccgcg gtcttcggaa agggcgcccc tggcgccgtc agctgggcat agaagtcctc
       61 gctcgacagg tccacgccgg cccggtactc acgttcgccg aacgtgacga gcaggggtac
      121 gacgctgatg ccggcgttct gtgcctgctc ggcggtgagg tcgcccgccg agtcggcgac
      181 gatcgcgacg cgcgtcaggg actagaccct ttcgacagcc atggcgacgg agccgccgcc
      241 gccgaggcac agggttgcga ggccgtacct gccgccgcgc cggcgcagct cgtggagcag
      301 cgtggccacg atgcgtgcgc cacttgcgcc gatcggatgt ccgagcgcga tggcgccacc
      361 gttgacgttg accttggacc agtcgaatcc gagctcccgg ccgtcggcaa gcacctgtgc
      421 cgcgaaagcc tcgttgatct cgattaggtc aaacgcctcg atcggcatct caatgcgagc
      481 aagcagcttc tggactccct tgacgggcgc aaggaatagc cacttcggct cgacctctgc
      541 ctgggcatag ccgacgatcc gcgcgagcgg tgtcagtccc aaacgttcca cggcgcgctc
      601 actggcaacc actgtggcag cggcaccgtc cgtgataccc ggcgcatttc cggctgtaac
      661 agtgccgccc ggcaggccga cggtggagtc cgggtctggg tccggcagcg caaagacggg
      721 cttcagcttg cccagcgcct cgaggctgct gtcgggccgc ggcccttcgt cgaccgcgaa
      781 ttcgaccatc tccttgcctt tcttgaccgg cacggggacg atctcctcgg cgaagcggcc
      841 gtccttgata gcggcaaggg cccgctggtg ggactccaag gcgaactggt cctgatcctc
      901 gcggctgacc tggttcttga tcgccacgcg ctcggcgtgc gtgcccatgt ggcacgactc
      961 agtggagcac cacaggccgt cgtgaaccgt cgcgtcgatc atctccgcgt tgccgaggcg
     1021 atagccgaac cgcgccttcg ggatgagata cggccccagg ttcatgttct ccatgccgcc
     1081 ggccacgacg atctcagcat caccggcgcg aatctcagcc gcggcaagca tgatcgcctt
     1141 gaggcccgaa ccacagacac gattgatcgt cgtcgcgctg gtcccctccc ccaggcccgc
     1201 cttcagggcc gcctggcgcg cgggcgcctg gccgactccc gcctgcagca cctggcccat
     1261 gaggacctcg tcgatgggcg tgccctcagg gagaccggcg cgctcgaccg cagccttgat
     1321 cgcgatgccg cccaacgtgg tggccggcac gtcggcgaga gagcccccga acttgccgat
     1381 cggcgtccgt gccgccgaaa cgatgaatac ctgtccgttc cgatctacct gtcgcaaagc
     1441 tgatgtcacg ggtcgctcag ctgcttgcaa gtgaactcca caggtcggtc actgtagccg
     1501 tgtccctgca gatctacgag aagatgcaaa ggccgccgag gtcggctgaa ctgcagcgca
     1561 gaggagctcg cccgtcgagg agcacatgac gccgtaaggc cagacaaagg gttgccctcc
     1621 ctcggcagct cgacacggcg aaggaatgcg gcctcctctg agacatagag ggccttcaag
     1681 aactgtcggc tccactccgg tccggtgcgc aattcatcga gggctacgat ggtacagagc
     1741 gtgtgccacc caggggagaa ggaccgatga cgcaagccga gctgcagatc atcaacggag
     1801 tcgatgtcgg agagctaaac gaatacatcg agtcatgccg gcttgatcca gcgcgcgcgg
     1861 accgtaaccc ggttgtcgtg gcccgctggg tgggcggcgg acgtgccgag gtgacgtcga
     1921 gcctcggcgg agcgccggtg tacatgggcg gcccgcggga tccgagcgcg ctgggaatgc
     1981 ttttgcggag ccttgcggcg tgtgacgtgg agatcgtggc gacgacagct gccagactcg
     2041 gtctcgagat cgaggatctc tcggtggagg cccggggctt cgccaatgtt gggcgctacc
     2101 tcgggctcga ttcagcgctt tctcccggat accagaacat cgcctactcg atccggctga
     2161 agacgaaggg cgacgcgact cccgaacagc tcgatgccat caggcgcgct tgcgaggaag
     2221 ggtcaccagt cggcgacacg ctgaagagac ctattccgct cgtgctcgaa ttcgacgcga
     2281 actgacctgg gctcggaacc tcaatggcga gactgcaacg acgtcggttc agcgagagct
     2341 ccgacgtcag gtcgttcccg cacgggaagg ttgaagtcgt tgagctagat gaccgcgctg
     2401 ttggtcgcct cacatacgag ccgggctggc ggtggtccgt cgatgtcagg ccgatcgcgt
     2461 caacccagtc gtgccagttc caccacgtcg gcgtgaccgt cagtgggcga ctgagagttc
     2521 tgatgcagga tggagtggag ctggaaatcg gcgccggcga cgtcttcgag atccctcccg
     2581 ggcacgacgc gtgggtcgtc ggcgacgagc cctggatctc gatcgacttc gaagcgatgc
     2641 gcagctttgg ccgcgcgacg ggtgacaaca gccggcgcgt cctgtcgacg attgtgatca
     2701 ccgacatcgt cgattcgacc acccgcgcgc acgctgtcgg accggcgcgc tggcgcgaaa
     2761 tcgtcagcca gcacaacgag atctccgagc ggatcatcga tgcctaccac ggccggttgg
     2821 tgaagacgac cggcgacggc gtcatcggcc tgttcgacag ctcggaacgg gcgattcgtg
     2881 cggctttgca gttgagcgac gcaatcgcgg cgcttgacat cgcgatccgc gcggccgtgc
     2941 atagcggcga gattgaaatt caggccaatg acattcgggg ggtggccgtg cacaccgcgg
     3001 cgcggatgat gaccgtcgcc gggcctggtg acgtgatcgt gtcaagcacc gtgcgcgagc
     3061 tgttggacga ctcggacgtc gctctcgagg atttcggcac gcaccagctc aaaggtctgc
     3121 ccggccagcg tcagcttttc cgcgtggtcg ctcgacgagg tcggtccgta ccacctgctg
     3181 cagcgcactg accagaccgc agctgcctgg gcctcgccgc aattggattg gcgatcaggc
     3241 gccgagggat agcattcagg cgtgccccag cagacgctga ccgaggcgca gctcagagaa
     3301 cgtggcctgg cgggcctggc cgagcttgcc cgtcttgacg agcgcaccgg gtcgatcaac
     3361 gcggtggcgg cgcgggcact tgacctcgtg ggctcgctcc tggacgccga ccgcgccgcc
     3421 gtgtgtgcca tcgacggcga cgagaccatc gagtggctgg cccatcgccg gatggagcga
     3481 ttcatcgcgg cttcggcaga cctacgaccg agcgacctcc tgtgggcacg tcgcccgctc
     3541 gagacgggac gaccagagct cttcgaccgc cgcgccccat cccacgaacg aagtgccctc
     3601 tccgacacgg cagacgccct gggcatggcc gcctttgcca tcgtgccact ccggtccggc
     3661 caggagttca ccgggatcct gggcctcgtc tggaacgacg atccacccga gctgggcgcg
     3721 gacgaggagc tgttgacgac catcggtcgg ctggtggcgc tggcgctggg caacacgcgg
     3781 ctacgcgaca cattgctcgc gcgccagtcg gagctcgacg aatcggaagc gcgatatcgg
     3841 agcctcttcg agcaagcgcc cgagccgatg ctcatctgcg actgggacgg cgccgtgctc
     3901 gacttcaacc gcgccgccat ggcgctctac ggcgtgaccg ggcaccgcct gctgaaccac
     3961 cgactggaaa cgctgaccga catcagcctc gaccgccggc ggcgcctcgc ggctgcgctc
     4021 caccgctccc gccgggcgga ggtcgaggca cgcgggcggc gccgggacgg ttccgagttc
     4081 ccgatggtgc tgcacctcgc agtcatcgcc ttccgcggtg aggagcggct gctggtccag
     4141 ctgcgcgaca tgaccgatca gcaacgcatg caagccgagc tcttgcaggc gcagaagatg
     4201 gaggcgctcg gccagctcat cagcggcgtg gcgcacgagc tcaataaccc cttgagcgcg
     4261 atcatcgcgt tctcgcagct attgcggtcc gatgagcgcc tgccggccga tctggcacgc
     4321 gacgccgacc tgctggtggg cgaggccgac cgcacacgac gaatcgtcca gaacctcctt
     4381 gacttcgccc gtcagcgcac gcccgaccgc cagccgacct cactcccggc cctggtcgag
     4441 cggacgcttg agctgcacgc gtacgcgctg cgggcagggc gaatcgagac acgtgtcctg
     4501 atgccgaccg atctgcccga cgtgtgggcc gactccgccc agatacagca ggtcctgtta
     4561 aacctgaccc tgaatgccat ccagtcgatc cgggcgtcca acccgaacgg cacgctgacc
     4621 attgccgcgc ataccgagcc gcgctcggac ggtccacgat tcgtccgtct acgtgtcgag
     4681 gacgacggcc ctggtgtcga actccaatcg cgcgaccacc tgtttgagcc gttcttcacg
     4741 acaaaggacg tcggccaggg cacgggtttg ggcctgtccg tcagctacgg catcgtcgcc
     4801 gcgcacgggg gcaagctctg gtacgagccg gcttcggtgg ctggcgcgac tttcctgttg
     4861 gagctaccag ctgcggcgga catcgaacga actctcgctg gcaagagtga acggcctgcg
     4921 acagcggctg ccaaccgggg cttgggcagc atggtcctcg tggtcgacga cgagcgcgcg
     4981 gtccgcgaga ccattcgtcg cgccctcgag cgtgccggac attcggtgac cgttgcgggc
     5041 tccggggcgg aggccttgga aatcctgcgt gatcggtcgg tcgatctcct gctcatcgac
     5101 catcgcatga gtgccatgga cgggacagag ttctatggct atgccatcgc cgcacggccg
     5161 gagctgacgg gtcgagcgct catgatgacc ggcgacacgc tcaaccccga gctgctggaa
     5221 ttcgtccgcg agaactcgct gagcctgatc gccaagccgt tcgacatcga cgacctgact
     5281 gcggaggttg actccgccct cgcacctgcc taggagccgc taccccttct tgggctcgcg
     5341 cgggtacgtg tagaagccgc gaccggtctt ctggccgaga tggccggcgg caaccagctg
     5401 acgcaagatc ggggggggcg ccatgtgcgc cgcgccgaaa ccgtcctgga gaacctccat
     5461 gatgtggagg cagacgtcga ggccgatgaa gtcagcgagc tcaagcgggc ccatagggtg
     5521 gttgaggccg accttggcgc cggagtcgat gtcctcggct gtacccaggc cctcctcgaa
     5581 ggcacgcatc gattcgccga ggtatggcat caagatccgg ttgacgatga agcccggccg
     5641 gtccttgctg acgatgacct gcttgcccaa ctcggcggcg acgtcacgga cataggcctc
     5701 tgtcgcgtcg tccgtctcgg cgccacggat cagctcgatg agcggcatca ctggcacggg
     5761 gctgaagaag tgcatgccca cgaacctggc gcggcgcccg gcaccggtgg cggcggccag
     5821 tcgcgtgatt gatatcgagc tggtgttgct ggcgaggacg ctgctgggtg gagcggcagc
     5881 gtcaatctcc gcgaatagcg actgcttgac agcctcgtcc tcgaagatcg cctcgaccac
     5941 gagctcggct tgcgacgccg cctctgcgag cgaggctgcc acgctgaccc ggcccagctg
     6001 agcggtccga tcctcgtcgc tgatcttgcc cttcgccacg gcgcgctcga gattgccggc
     6061 gatccgttct cgcccggcct cagcgcgtgc tgcttccggt tcgtagagcc acacgcggtg
     6121 gccggccgcg gccattacct gggcgatgcc gtggcccatc aggcccgcgc cacacacgag
     6181 caccttacgt tcgctcacgg cgggaagcct agcgcagctt cagctgttgt cggcgagctt
     6241 cgcgagcagc tcgtcggcag ccgaatacgg gtcctgctca tgccgtgcga cgcgtgtcac
     6301 gacgtccgct gccgccgcgc gatgggcggc ggtggccagg cgcgtgcgta atcggtcagc
     6361 catgattcct gaaagttggg cttcggcgcg cttctgagcc gcggccaacg cgacggacgg
     6421 atctgagtcc gcggcacggc gggcggcagc gcgatgatca agtgcggcca gaagctcggg
     6481 cacgccggcg ccggtcatcg ccgacgccag cagaacgtcg ggccgcttcg gacgcgggcg
     6541 gtcgcccatg gcccgatcgt gctgggcgcc aacggtcagc atgccccgca gctgcgcggc
     6601 ggcgcggtca gcgccgggtc gatccgactt gttgacgacg acgatgtcgg ccacctccag
     6661 caatccggcc ttgatggcct ggacctcgtc gcccatctcc ggtgcctgga cgacgagcgt
     6721 cgtgtcggcg atggcggcga tctcaacctc gctctgcccc gtgccgacgg tctcgatgaa
     6781 gatcagcccg aaccccgatg cgtcgaacgc cgccgcggcc gcgatcgttg ccgcggcaag
     6841 gccgccggag tggccgcgcg acgccatcga gcggatgaac acgtcggtgt cgctggcatg
     6901 ctcctgcatg cgcacccggt cgcccaggat cgccccgccg gtgatcgggc tggatgggtc
     6961 gattgcgatg acggcaaccg gtcggccgct cttgcgtgcc tcacccacga gcgcggcaac
     7021 caacgtggac ttgccggaac caggcgctcc ggtcacgccg acaagatgcg ccgaaccggc
     7081 gtgcgggtac agctcgcgga gagcgtcctc accggcggcg gtccggttct caacgtgggt
     7141 tagcagccgc gccagcgccc gccggtcgcc gcctcgagcc cgttcagcga ggctccggtc
     7201 ggcgcttgcc gtcacccctc gcgccgtgcg acgttgctgc ggaagaactc ggcgacttca
     7261 cgaatcgtcg tccccgggcc gaagacgcgc gcaacgccaa cttcgcgcag ctgtggaatg
     7321 tccgcgtcgg ggatgatccc gccggccgtg accagcacgt cgtccatgcc ctgttcgcgg
     7381 agcgcggccg tgatcttggg aagt
//
LOCUS       DATLCO010000291         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6254984, whole genome shotgun sequence.
ACCESSION   DATLCO010000291 DATLCO010000000
VERSION     DATLCO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6254984"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VMZ33_06705"
     CDS             <1..1321
                     /locus_tag="VMZ33_06705"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16957.1"
                     /translation="MGETMRDFDRPVLLGGTRGPGLGQRIRGHPYVRLARDARFAAFW
                     LAQTISLFGDRLHQVALGVLVYAITNSPLATGLVFFAATLPNIFLGPIAGTFVDRWEH
                     KRVLVASDFIRAGLVLLIPFAAAANVIYVYPLVFLITTVSLFFRPAKIALLPRIVRED
                     DLLAANSATWTADTIADIAGFPLAGLFVAVLGTEASQLGLAFFADSASYVLSGLLIAA
                     VAVAPLIRDKAAAVGNPVRNFTKELAEGWRFLRGQPALFQNTLISTVAQMTVGVTLAL
                     TVVYARDVLDGTLIPYPQNYAAIETAIGVGNLVGGLAVGIIGARLRKGWMVVGGFIAM
                     GLATVLFGLTGSVAVALALAFVIGIANLVYIVPAQTMFMELTPNELIGRVIAFRGSLV
                     FGSMTLAMAIAGVLAESVSAGLVIAGFGALMTLAGLVGALLPAVRDA"
     gene            1341..1742
                     /locus_tag="VMZ33_06710"
     CDS             1341..1742
                     /locus_tag="VMZ33_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16958.1"
                     /translation="MIPESLQFLAVALAVLALVALVVALVLWRRTRGVTIPSAALVQR
                     LDERAQRLPAAIGTARANLAERTATIEHGLWLMARADAQIEATTVALRQRRVSLDQLR
                     EKMERSRARAETLKSTARMIIRALELRRTFL"
     gene            1743..2402
                     /locus_tag="VMZ33_06715"
     CDS             1743..2402
                     /locus_tag="VMZ33_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16959.1"
                     /translation="MTDPVRPEDQDELEATDAEPSANDAAEADAQVEVGAPVEADPVA
                     EAELVTEAEMAAEAEAEADVEAEAEAPMEAQTEAQAAADEFEPFDEPQVVAPAKSVPT
                     SSKAGSIVKAPASDETPYVDDRLSKFWVAAIALTFLAIFVYGFLFGTGGFLTARPTPL
                     PSESAAPSLSASPQASRTPAASRTPAPSTSPAPSTSLAPSPTSIPSAGGTVTPTGSPA
                     T"
BASE COUNT          398 a          871 c          798 g          428 t
ORIGIN      
        1 aatgggggag acgatgcggg acttcgaccg gcccgtcctg ctcggcggca cacgcggccc
       61 gggcctcggc caacgcatcc gtggccaccc gtacgtccga ctcgcccgcg acgcacgctt
      121 tgcagccttc tggcttgccc agacgatcag cctcttcggc gaccgcctcc accaggtggc
      181 gcttggcgtc ctcgtctacg ccatcaccaa ctcgcccctc gccaccggcc ttgtcttttt
      241 cgcggcaacc ctcccgaaca tcttccttgg cccgatcgcg ggcacgttcg tcgaccgctg
      301 ggagcacaag cgcgtcctag tcgcaagcga cttcatccgt gccgggctgg tgctgctcat
      361 cccgttcgcc gccgcggcca acgtgatcta cgtctacccg ctcgtcttcc tgatcacgac
      421 ggtcagcctc ttcttccgcc cggccaagat cgcgctgctg ccgcgcatcg ttcgcgagga
      481 cgaccttctg gcagcgaaca gcgcgacctg gacggccgac acgatcgccg acatcgccgg
      541 ctttcccttg gccggcctct tcgtcgcggt cctcggaacg gaggccagcc agcttggtct
      601 ggcattcttc gccgactctg cgtcgtacgt ccttagtggc ctgctgatcg cggcggtcgc
      661 cgtggcgccg ctcatccgcg acaaggcggc agccgtgggc aacccggtcc gcaacttcac
      721 gaaggaactg gccgagggtt ggcgcttcct ccgcggccaa ccggcgctct tccagaacac
      781 gctgatcagc acggtcgcgc agatgaccgt tggcgtgacc ctcgcgctga ccgtcgtcta
      841 cgcgcgggac gtccttgacg gcacgctcat cccctatccg cagaactacg ccgcgatcga
      901 aacagcgatc ggcgtcggca acctggtcgg cgggctggcc gtcggcatca tcggcgcccg
      961 attgcgcaag ggctggatgg tcgtcggcgg cttcatcgcg atgggcctgg cgaccgtgct
     1021 cttcgggctg acgggtagcg tggccgttgc cctcgcactg gcgtttgtga tcgggatcgc
     1081 aaacctcgtc tacatcgtgc cggcgcagac gatgttcatg gaactcacgc cgaacgagct
     1141 gatcggccgc gtgatcgcgt tccgcggcag cctggtcttc gggtcgatga cgctggcaat
     1201 ggccatcgcc ggcgtgctcg cggaaagcgt gtcggcaggg ctggtaatcg ccggattcgg
     1261 cgccctgatg acgctcgccg ggctcgtggg cgcgctgctg cccgcggtgc gcgacgcgtg
     1321 agggttgata atcgcctgca gtgatccccg aatcgctcca attcctagct gtcgctctcg
     1381 ccgtgttggc gttggtggcc ctcgtagtcg cgctggtgct gtggcgacga acacgtggcg
     1441 tgacaatccc ctctgcggcc ctcgtgcaga ggctggacga acgcgctcag cggctgccgg
     1501 ccgcgattgg caccgcgcga gcgaatctgg cggagcgcac tgcgacaatc gagcacggac
     1561 tgtggctgat ggcacgggcc gacgcacaaa tcgaggccac gaccgtcgct ctgcgacaac
     1621 gacgagtctc tctggatcaa ctgcgcgaga agatggaacg aagtcgggcg cgcgcagaaa
     1681 cactgaaatc gacagcgcgg atgatcatta gggcgctcga actgaggagg acgtttcttt
     1741 agatgaccga tcctgtgcgg ccggaagacc aagacgagct cgaggcgacc gacgccgagc
     1801 cgtctgccaa cgatgccgcg gaagccgatg cacaggttga agttggtgcg ccagtcgagg
     1861 ccgacccggt agccgaagct gagctcgtaa cggaagctga gatggcggcg gaagccgaag
     1921 ccgaagccga cgtcgaggca gaggcggaag ctccgatgga ggcgcaaacc gaagcccagg
     1981 ccgcggccga cgagttcgag ccgttcgacg agccccaggt tgtcgcgccg gcgaagtccg
     2041 tcccgacctc gtccaaggcc ggctcgattg tcaaggcgcc cgcttccgac gaaacgccat
     2101 acgtcgacga ccgcctttcg aagttctggg tcgcggcaat cgcgcttacg ttcctggcaa
     2161 tcttcgtcta cggcttcctc ttcggcaccg gcggcttcct gaccgcgcgc ccaaccccgc
     2221 tgccgagcga gtccgctgct ccgagtctgt cggccagtcc acaggcaagc cgcacaccgg
     2281 cagcgtcgcg tactcccgcg ccgtcgacca gccctgcgcc gtcaaccagc ctcgcaccat
     2341 caccgacgtc cattccgtct gccggcggga ccgtaactcc cacggggtcg ccagccacgt
     2401 aaccccatgc gccgctggtt gcgacggtcg agcggttccg actggatcgc cgaacagctc
     2461 gagcggcgcg gcgtacgaga tccggacgtc ctgcg
//
LOCUS       DATLCO010000292         2733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8494126, whole genome shotgun sequence.
ACCESSION   DATLCO010000292 DATLCO010000000
VERSION     DATLCO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8494126"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VMZ33_06720"
     CDS             complement(<1..387)
                     /locus_tag="VMZ33_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16960.1"
                     /translation="MTGGLIDRGAKFAGYIGLGMALVIAIAFELIIPVQTVVFLLCAP
                     MGLLIGVYANVKSERWRPRRRVFANSMYAGVVTGIGIAVLYTVIRFVFLYGDTGALPD
                     GTSLACRMGPDCTYQRYVALDQTDELA"
     gene            complement(384..1694)
                     /gene="eno"
                     /locus_tag="VMZ33_06725"
     CDS             complement(384..1694)
                     /gene="eno"
                     /locus_tag="VMZ33_06725"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HUR16961.1"
                     /translation="MTTIALVDALEVLDSRGNPTLEAVVVLDSGAVGRAIVPSGASTG
                     AHEAVELRDEEKERYGGKGVRKAVGNVIEMIGPALVGEDASAQADIDQLLIDIDGSTN
                     KANLGANATLGVSLACAHAAAAEYGLPLYRYLGGVAATILPVPMFNILNGGKHALDST
                     DFQEFMVMPIGAPTYSEALRAGSECFHALKTLLHDGGFATGQGDEGGFAPSLKSNQEA
                     IEMVLRAIEHAGYKPGDEVAIALDPAVTELVDLDTAADSNGELTYRLAKENRTLRTGE
                     MIDFWADWLDRYPIVSLEDGLAEDDWAGWTALMKRLGSRVQLVGDDLLVTNVERVQRA
                     IDEDAANAVLVKLNQIGTLTETLATIQLARSHGWGAVISHRSGETEDTTISDLAVATG
                     TGQLKSGAPSRSERVAKFNRLLRIEAELGLSARYAGASALAVSR"
     gene            complement(1691..>2733)
                     /locus_tag="VMZ33_06730"
     CDS             complement(1691..>2733)
                     /locus_tag="VMZ33_06730"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HUR16962.1"
                     /translation="MSHLGRPKGKVVESARLRPVAQRLSTLLRMPVPITGDPLGIGTR
                     DAVARLKAGQVLMLENLRFHAAEENNDDEYAKTLATYGQVYVNDAFGTAHRAHASTVG
                     VTKHLPAYAGLLMEREIQALSSLLGDVKRPFATVIGGAKVSSKLAVLKNLMQKVDSIL
                     IGGGMANTFLVAQGRTVGKSLLERELVDDARQILEDGDKHGVQILLPTDVVVAKEVTR
                     GAEHKIVPVHKIPNSWSTVDIGPQTTEAFTQALEGAQTIFWNGPLGVFEVPTFGDGTR
                     AMGRYLAQRAEDGATVIVGGGDSIAALEELGLTEKMSHISTGGGASLEFLEGRELPGI
                     AVLLDREPAAIA"
BASE COUNT          458 a          920 c          885 g          470 t
ORIGIN      
        1 ggccagctcg tccgtctggt cgagagccac gtaacgctgg tacgtgcaat cgggccccat
       61 tcggcaagcg agggacgttc cgtcgggcag cgcaccggtg tcgccgtaca ggaaaacgaa
      121 gcggatcacg gtgtacagaa ccgcaatccc gatgccggtt acgacgcctg cgtacatgga
      181 gttggcgaat acgcgacgcc gcgggcgcca gcgctcggac ttgacattcg cgtagacgcc
      241 gatgaggagg cccatgggcg cgcacagcag gaagacaaca gtctggacgg gaatgatcaa
      301 ctcgaaggcg atcgcgatga ccagggccat gcccagtccg atgtagcccg cgaacttcgc
      361 gccgcgatcg atgagcccgc cggtcacctc gacactgcca gcgccgatgc gccggcgtag
      421 cgggcggaca acccgagctc agcttcgatt cgcaacagcc ggttgaactt ggcgacccgc
      481 tccgaacgcg acggtgcgcc gctcttcagc tggccggtgc cggtggcaac cgccaggtcg
      541 gagatcgtgg tgtcttccgt ttcgccgctg cggtgggaga tcacggcgcc ccagccgtgg
      601 ctgcgtgcca gctggatcgt ggccagagtc tccgtcaggg tgccgatctg gttcagcttg
      661 accagcacag cattggcggc gtcctcgtcg atcgcgcgct gcacccgctc aacgttcgtg
      721 accagaaggt cgtcgcccac caattgaacg cgtgagccca gtcgcttcat cagcgcggtc
      781 cagcccgccc aatcgtcctc ggcgaggcca tcctcgagcg agacgatcgg ataacggtcc
      841 agccaatcgg cccagaagtc gatcatctcg ccagtccgca gcgtgcggtt ctccttggcc
      901 agtcggtagg tcagctcacc gttcgaatcg gcagccgtat cgaggtcgac cagctcggtg
      961 accgcgggat ccagggcgat cgcgacctcg tcgccgggct tgtagccggc gtgctcgatc
     1021 gcgcgcagga ccatctcgat cgcttcctga ttcgacttga gagagggtgc aaagccaccc
     1081 tcgtcgccct gtcccgtggc gaagccgcca tcgtggagca gcgtcttcag cgcatggaag
     1141 cactccgacc cagcacgcaa cgcctcggag tacgtcggcg cgccgatcgg catgaccatg
     1201 aactcctgga agtcggtcga gtcgagcgca tgcttgccgc cgttcaggat gttgaacatc
     1261 gggacgggga ggatcgttgc cgcgacgccg ccgaggtagc ggtagagcgg cagtccgtac
     1321 tcggccgcgg ccgcgtgcgc acaggcgagc gacacgccca acgttgcgtt ggcgcccagg
     1381 ttggccttgt tggtcgaccc gtcgatgtcg atcagcagct ggtcgatgtc tgcctgtgcg
     1441 gaggcgtcct cgccgaccaa cgcagggccg atcatctcga tgacgttccc tacggccttc
     1501 cggacaccct tgccgccgta gcgttccttt tcctcgtcgc gcaactccac ggcctcgtgc
     1561 gcaccggtgg aagcgcccga aggcacgatc gcccgcccca cggcgccaga atccaggacg
     1621 acgacggcct ccagcgtcgg gttgccacgc gagtcgagga cttcgagcgc gtcgacgagt
     1681 gcgatggtgg tcatgcgatt gccgccggtt cgcggtcgag gagcaccgca atccccggga
     1741 gctcgcgccc ttccaggaac tcgagcgacg ccccgccacc ggtcgagatg tggctcatct
     1801 tctcagtcaa gcccagctcc tcgagcgcgg cgatcgagtc tccgccgccg acgattaccg
     1861 tcgctccgtc ctcagcgcgc tgcgccaggt agcgacccat cgcccgcgtg ccgtcaccga
     1921 acgtcggcac ctcgaacacg ccgagtggcc cgttccagaa gatcgtctgg gcaccctcga
     1981 gagcctgggt gaaagcttcg gtcgtctgcg gaccgatgtc cacggtggac caggagttcg
     2041 ggatcttgtg cacgggcacg atcttgtgct cggcgccgcg tgtgacctcc ttggcgacca
     2101 ccacgtcagt cggcaggagg atctgcacgc cgtgcttgtc accgtcttcg aggatctgcc
     2161 gcgcgtcgtc gacgagctcg cgctcgagga ggctcttgcc gacagtccgg ccctgcgcga
     2221 cgaggaatgt gttcgccatg ccgcccccga tgaggatcga gtcgaccttc tgcatcaggt
     2281 tcttgagcac ggcgagcttg cttgagacct tggcgccgcc gatgaccgtc gcgaacggac
     2341 gcttcacgtc accgagaagc gacgacaggg cctggatctc acgctccatc agcagtccgg
     2401 cgtacgccgg caggtgcttc gtcacgccga cggtcgaggc atgggcacga tgcgccgttc
     2461 caaacgcgtc gttcacgtac acctgaccgt aggtcgcgag ggtctttgcg tactcgtcgt
     2521 cgttgttttc ctcggccgcg tggaaacgca ggttctcgag catcagtacc tggcctgcct
     2581 tgagacgcgc gacggcatca cgcgtgccga tcccaagtgg gtcaccggtg atgggcacgg
     2641 gcatccgcag caatgtcgac aggcgctgag cgacgggccg caggcgggcc gactcaacca
     2701 ccttgccctt gggtcggccg aggtggctca tca
//
LOCUS       DATLCO010000293         3492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7744298, whole genome shotgun sequence.
ACCESSION   DATLCO010000293 DATLCO010000000
VERSION     DATLCO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7744298"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1121)
                     /locus_tag="VMZ33_06735"
     CDS             complement(162..1121)
                     /locus_tag="VMZ33_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HUR16963.1"
                     /translation="MLTIQDIRKAATRLSGIAVHTPLLPFRDGAFIKPESLQPTGSFK
                     IRGAYNALSQLSAEERARGVVAHSSGNHGQAIARAARLLGMRAIVVMPNNAPAVKVAG
                     VRADGALIEFVGPHNVERVERAHQIAHDDGLILVPSANHEDVICGQATIGLEIVEELR
                     ARGITDPPVVIVPVGLGGLAAGVGLAVMSLSEDASVFGAEPALAADTHESIDRGERVA
                     WPAEQTARTIADGLRGESPAEIAFALLKQHLTDVITVSEDEIVEAMRVGAREARLVLE
                     PSGATSLAAYLSRVGELPAGPVVIVVSGGNVDQNLYASWLAAG"
     gene            1217..2380
                     /locus_tag="VMZ33_06740"
     CDS             1217..2380
                     /locus_tag="VMZ33_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HUR16964.1"
                     /translation="MGEERARRRHSKADTLRRTYGFDDVSLAPGTETLDPADVDLAVE
                     IGGLKLTMPVLAAAMDAVVDSDFCGAMAKLGGLAVFNLEGVQARYDDPAAVLAEIAHA
                     PDDRVQDVLAEAYKAPVRDDLVARRIAEIHASGSPAFVAATPAGARRLGPLSAEAGAD
                     VFLVQSQVSSARHLAAGYEPLALADFTKNMPMPVCVGNTTSYQAAIELMRQGAAAVFV
                     GVGPGAACTTRDVLGIGVPQVTAIADVAAARDDFLDQTGRYVAVVADGGMRRGGELAK
                     AIAAGADLLMLGSPLARAEEAPGRGTNWGMAAPSPTLPRGVRIKVGTVGSLEKILLGP
                     ATVSDGTQNLVGALRQSMAALGAGDIRAMQLVEMVYAPAVAVEGKSWQRGSSR"
     gene            2356..2775
                     /locus_tag="VMZ33_06745"
     CDS             2356..2775
                     /locus_tag="VMZ33_06745"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HUR16965.1"
                     /translation="MATRIEPLIDPSHAEGADRSIGEELVGWGKVASIETVGRMTGTR
                     VTAAVGFIEDADGSLLVAAGNETADWALNLRAEPRTRVTIGDTSATYLAAEANDTEKA
                     RAVTELILKYGTPAERLGRGPVFRLRPTGPTRSAGPG"
     gene            2772..3275
                     /locus_tag="VMZ33_06750"
     CDS             2772..3275
                     /locus_tag="VMZ33_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16966.1"
                     /translation="MTDDDQSAASSATFDDTERAIGQEEGGGGRGRSGCGVIGCVLII
                     VVLVALVAGTYVLGQRLEPLADRFLWAPHDVVREYLTAYEEGDLDRARGFLCAARQNA
                     GVPDPSEAVGSPRSWTAGVEDQFPYPRPDGQVGIYYRVTSGLGDQRAQALLVREEDGW
                     RICEFSG"
BASE COUNT          551 a         1167 c         1220 g          554 t
ORIGIN      
        1 tagacgatcg cggccgttgc gccagctgct ccaatcacgt gcagcgcgag cgcgtcgagc
       61 ttgctgtcga gctcgctgcc gcgggcaacg atgcgggcgg cgtcaaggag cgtcaggctc
      121 gcgtcggggg tgctcatctg ctctcgatga tagagcgcgg ttcagccggc ggcgagccag
      181 ctcgcgtaca gattctggtc gacgttcccg ccactgacca cgatgacgac cgggccggcg
      241 ggcagctcgc ccacgcggct gaggtaggcg gccagtgagg tggcgccaga aggttccaat
      301 acgagtctgg cctcgcgcgc cccaacccgc attgcctcga cgatttcgtc ttcggagact
      361 gtgatgacgt cggtgaggtg ctgcttcaac agggcaaagg caatctcagc cggcgactca
      421 ccgcgcaggc cgtcggcgat ggttcgcgcg gtctgctcgg ccggccaggc cacgcgctcg
      481 ccgcggtcga tcgattcatg cgtgtcagcg gctagcgccg gctccgcgcc gaagacgctt
      541 gcatcctccg aaagcgacat gacagccagg cccacgccag ccgccagccc gccgagacct
      601 acgggcacga tcacaaccgg tgggtcagtg atgcctcggg cgcggagctc ctcaacgatc
      661 tcgaggccga tcgtcgcctg gccgcaaatg acgtcctcat ggttcgccga cggcacgagg
      721 atcagcccgt cgtcatgcgc gatctgatgg gcacgctcga cgcgctcgac gttgtgtggc
      781 ccaacaaatt cgatcagggc gccgtcggct ctcacgccag caaccttcac ggcaggtgcg
      841 ttgttgggca tcacgacaat cgcgcgcatc cccagcagcc gtgcggcgcg ggcgatcgcc
      901 tgtccgtggt tgccgctcga gtgagcgact acgcctctcg cccgttcctc cgcgctcagt
      961 tgcgacaggg cgttgtatgc gccccggatc ttgaacgagc cggtcggctg gaggctttcc
     1021 ggtttgatga acgccccgtc gcggaagggc aacagcggcg tgtggacggc aatgccggag
     1081 aggcgcgtgg cggctttgcg gatgtcttgg atggtcagca cgacgcctat actgccgtga
     1141 ctgaccgccg cctgcgtggc ggccgccgtg gaggtcttcg tcgatcgtga atctcggtag
     1201 cgccgcaccc tccgacatgg gcgaggagcg cgcgcgccgg cggcattcga aggccgacac
     1261 cctgcgccgc acttacggct ttgatgatgt ctcgctcgcc ccgggaacgg agacgctcga
     1321 tccggcggat gtggacctcg cggtcgagat cggcggtctc aagctcacga tgcccgtact
     1381 ggccgcagcg atggacgccg tcgtggactc cgacttctgt ggcgcgatgg cgaaactggg
     1441 cggcctcgcg gtgttcaatc ttgaaggcgt ccaggcacgc tacgacgatc cagccgctgt
     1501 gctggccgag attgcgcacg cgcccgacga ccgcgttcag gacgtcctcg ccgaagcata
     1561 caaagcgccc gtgcgcgatg acctcgtggc gcgtcgcatc gctgagatcc acgcgagcgg
     1621 gtcgcccgcg ttcgtggccg cgaccccggc cggtgcacgt cgactcggcc cgctgagcgc
     1681 cgaggcaggc gccgacgtgt tcctggtcca gtcgcaagtt tcgtcggcgc gtcacctcgc
     1741 ggccggctac gagccgctgg cgctggccga cttcacgaag aacatgccga tgcccgtctg
     1801 cgtcggcaac acgacgtcgt atcaggccgc gatcgagttg atgcgccagg gcgctgccgc
     1861 ggtcttcgtg ggcgtcggcc cgggcgccgc gtgcacgacg cgcgacgtac tcggtatcgg
     1921 cgttccacag gtcaccgcca tcgccgacgt tgccgcggca cgtgacgact tcctggatca
     1981 gaccggccgt tacgttgccg tcgtcgcgga cggcgggatg cgtcgcggcg gggagctggc
     2041 caaggccatt gctgcgggag cggacctgct gatgctcggc tcaccgctgg cgcgtgccga
     2101 ggaagccccg gggaggggca cgaactgggg catggctgcg ccatcgccga cgttgccgcg
     2161 cggcgtccgc atcaaggtcg ggaccgtcgg ctcactcgag aagatcctgc tcggcccggc
     2221 caccgtcagc gatggcacgc agaacctcgt cggggcgctg cgccagtcca tggccgccct
     2281 cggggccggg gacatccgag cgatgcagct ggtggagatg gtgtacgcac cagcggtcgc
     2341 tgtcgagggc aagtcatggc aacgcggatc gagccgctga tcgaccccag tcacgccgag
     2401 ggcgccgatc gttcgatcgg cgaggagctc gtcggctggg ggaaggttgc ctccatcgag
     2461 actgtggggc gcatgaccgg gacccgagtg acggcggccg tgggattcat cgaggacgca
     2521 gacgggagcc tgctcgtcgc cgccggcaac gaaaccgccg actgggcgct caacctgcga
     2581 gcagaaccac ggacacgagt cactatcggc gacacgtcgg cgacctatct cgcggccgag
     2641 gccaacgaca cggaaaaggc acgcgccgtt accgagctga tcctcaagta cgggaccccc
     2701 gccgagcggc tcggccgcgg accggtcttc aggctgcgac cgacaggacc gacaaggtcc
     2761 gcaggacccg gatgactgac gacgaccaga gcgccgcaag ctccgcgacc tttgacgaca
     2821 cggagcgcgc gatcgggcag gaggagggtg gtggcggacg gggacgcagc ggctgcggtg
     2881 tcatcggctg cgtgctcatc atcgtcgtgc tcgtggcgtt ggtagcgggt acgtacgtgc
     2941 tcggacagcg gttggagccg ctcgccgacc gcttcctgtg ggcccctcac gacgttgtcc
     3001 gtgagtacct cactgcctac gaggaaggtg acctcgatcg ggcccgcggc ttcctctgcg
     3061 ccgcgcgcca gaacgcgggt gtgcccgatc cgtcggaggc cgttggctcg ccgcgctctt
     3121 ggacggctgg cgtggaggac cagtttccct acccgcgccc cgatggccag gtaggcatct
     3181 actaccgcgt cacctcgggg ctgggcgacc agcgcgcgca ggctctgctc gttcgcgagg
     3241 aagacggctg gcgcatctgt gagttcagcg gctgacccgc tcgaagagga agccgaccga
     3301 ctgaccgagt tcgaccgtgc gtaaaatcgg cccacggcac gccgtgcagc cagggaggac
     3361 acgctcgatg aacaagtttg cagccacagc gatcacatcg gggatgatgg ccatcgcggt
     3421 cgcttgcggt ggtgggtccg ccccaacgcg tgcaagcgtc gctccgagcc tcaccggtgg
     3481 gataccgcat cc
//
LOCUS       DATLCO010000294         4569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4296015, whole genome shotgun sequence.
ACCESSION   DATLCO010000294 DATLCO010000000
VERSION     DATLCO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4296015"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3572)
                     /locus_tag="VMZ33_06755"
     CDS             complement(<1..3572)
                     /locus_tag="VMZ33_06755"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HUR16967.1"
                     /translation="MAVDVAALLAAQRALDPLVIDLARALSVATRIETTLVRHMRLEL
                     FPDSDAGLEANLWFSTLVADRDMLGVSLIPECRDELRRQLTLPEHRQTLELAWRVIVN
                     CHRPADAAGVPPVINVQEATTYHALAAAAYGSASHRQSMQEGLGTLMKGLSEDSDGAL
                     TEWIVRSLPALPAAARQSSAAIALAERVRARDATAVLEGVPRVARDASGRPSIDWALN
                     TVAVGIQRRTDGIELTVPPAAGAHIFHVPNTAPLAIAIQSPTPEGWQTRSIDFEPLPR
                     QVIADPTPASVCRFTTASGQRLTVQPIFGETRVTLDVWMEGVDPSSLNDIPEQLRAMC
                     GELGWNLRWPAANLPFDSDPAAALFYLGEASAFTPPSPIVPVIAWARDRLEPVEGGAA
                     LTLTLEHESSVLSAEFSPDATRIVTGGDGSIRVWDASTGDLLQDRPLPVPSRAASFSP
                     DGKSLLLASNDGTVTLWPLDRPDPSVFGTHEDWVMSAEFGASGTTAVTASQDSTAAIW
                     NVESGAKRIVLRHDGAVNHASFSPDESRVATASDDQTAAVWSVDGRKLATLTHEADVR
                     SVQWSPDGLWLLTADADGNAWLWSASTFARDRRPFQHDFEIHFATFSSDGRRIAVATS
                     DGIAWVWHLPSRALLVPGLQAPIQTGQSNIPVKPSKRPRSVRLQAVYSVAFSPDGQRV
                     LTGGASGRAHVWDVSTAAPSNSVARRTTLRRMEFAPPAPEIVKGMKESLRAQLLALDE
                     RRLLWWTAHSPSVNEAAAHDPNPAAVSSPAEEPTPAQSTRPTIFVSYNHKDEEWFERL
                     ERVLAPVVRSQDIEFLTSEQIGAGAGSEAEIDAAMARAAVIIILVTPSYLASDYAIRE
                     LTRLADTAQSARSKIVWVPLRASNWDETPLSQYQAITDPSQPWSKLNRPAQDAAMTAL
                     VERVQQMVGATPDEAQTAGANPGSRIFLCYRRGRDSGRAGRLRDRLTDEFGESDIFLD
                     VSTLRPGVDFAEVIAQAVSAAAVMLVIIGPQWLEERDADGRRRIDSEQDWMRMEVSLA
                     LRSNVTVIPVLVEGASMPRASALPSDLRGLARRQAIELADDRWEYDVRQLIDAMRSSI
                     GDTLPDSKRAATPRRPIRIYLSSTHTDLKEHRERVVGALRRVSEFVIVNEPTLADERG
                     PVQRELQDLASADLMILIVALRYGYVPDDKQINPERLSMTELAF"
     gene            complement(3572..>4569)
                     /locus_tag="VMZ33_06760"
     CDS             complement(3572..>4569)
                     /locus_tag="VMZ33_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16968.1"
                     /translation="PRRMIDVNARNRGDIWISDVLEAWRTLQLETPEDRAAAAEALGF
                     DASRFRPSTETPASGAGSNMGSDDTGVGAHGKDSVSAPRAAVAASVIPSSAVQDQIAG
                     AADEGSWATNAPALLAEQAAHTVFKPAHLPLFRPHWTRHLMSSICAVPIAEGAVDEPR
                     LIDAVTRLRPLVRLPLRPRATLRRGVHVLVDRSESMMPFTRDVDDILRSLRATIGKDQ
                     FATAAFVDTPLAGDVLWLRTRIRAPWQAPSAGTRILLISDLGLAQHQGEIRGALPAEW
                     LVFAARCRAAGCHTVAILPRHPDSCPPALRKVFDVVPWDARTNVATVLRSVRART"
BASE COUNT          744 a         1534 c         1535 g          756 t
ORIGIN      
        1 ttgaacgcca gttccgtcat cgacagtcgt tcggggttga tctgcttgtc gtcgggaaca
       61 tacccgtaac gcagcgcaac gatcagaatc atcaggtcgg ctgacgcgag atcctggagc
      121 tcgcgctgca cgggaccgcg ctcgtcggcg agagtcggtt cgttgacgat cacgaactcc
      181 gatacgcgcc gaagcgctcc caccactcgc tcccgatgct ctttgagatc ggtgtgggtc
      241 gacgagagat agatgcgaat gggccggcgg ggcgtcgcgg ctcgcttgga gtctgggagc
      301 gtgtcgccga tggacgaccg catcgcgtct atcagctgtc tgacgtcgta ctcccaccga
      361 tcgtccgcga gttcgattgc ctggcgccgc gccagcccac gcagatctga cgggagcgca
      421 cttgccctcg gcatgctggc gccctccacc agcaccggga tgaccgtcac gttcgacctc
      481 agcgccaggc tcacctccat gcgcatccag tcctgctccg aatcgatgcg acgtcgtcca
      541 tccgcgtcgc gttcctcgag ccattgcggc ccgatgatca cgagcatgac cgccgcggct
      601 gatacggctt gcgcgatgac ctccgcgaag tcgacgcctg gccggagtgt gctcacgtcc
      661 aggaatatgt ccgactcgcc gaattcgtcc gtcagccgat ccctgagccg gccggcgcgt
      721 ccgctgtccc taccgcgacg atagcagagg aaaatgcgtg aacctggatt ggcccctgcc
      781 gtctgcgcct cgtcgggcgt tgctccgacc atctgctgca cccgctccac gagcgcggtc
      841 atagctgcgt cttgtgcagg acgattcagt ttcgaccagg gttgtgaagg atcggtaatt
      901 gcctgatatt gcgacagcgg cgtttcgtcc cagttggacg ccctgagtgg gacccacacg
      961 atcttcgacc gtgcggattg agcggtgtcc gcaagccgtg tgagctcccg aatggcatag
     1021 tcggacgcca ggtacgacgg tgtcacgagg atgatgatga cggctgcacg agccatcgcg
     1081 gcgtcgattt ccgcctccga cccggcaccg gcgccgatct gttcgctggt caagaactcg
     1141 atgtcctggg atcgcaccac gggggccagg acacgttcca gacgctcgaa ccattcctcg
     1201 tccttgtggt tgtagctcac gaagatcgtg ggccgggtcg actgggccgg cgtcggttcc
     1261 tcagccggtg aagagacggc ggctggattg gggtcgtgcg ctgcggcctc attgacggac
     1321 ggtgagtggg cggtccacca gaggaggcgg cgctcgtcga gcgccagaag ttgcgcgcga
     1381 agcgactcct tcattccctt gacgatttcc ggagccggcg gcgcgaactc cattcgccgg
     1441 agcgttgttc gcctcgcgac cgaattgctc ggggccgccg tcgacacatc ccacacgtgc
     1501 gcgcgaccgc tggctccgcc cgtcaacaca cgctgaccgt cgggcgagaa cgcgacgctg
     1561 tacaccgcct ggagccgtac ggaccgcggc cgtttcgagg gcttgaccgg aatgtttgat
     1621 tgcccggtct ggattggcgc ttgtaatccc ggcacgagga gggcgcgcga cgggagatgc
     1681 cacacccacg cgatgccgtc ggaggttgcg acagcgatcc ggcggccatc cgaactgaac
     1741 gttgcgaaat gaatctcgaa gtcgtgctgg aacggacgtc ggtcgcgcgc gaaggtggac
     1801 gcactccaca gccacgcgtt gccgtcagcg tcggcggtca gcaaccacag gccgtccggg
     1861 ctccactgca cggaacggac gtccgcctca tgagtgagcg tggccagctt ccggccatcc
     1921 acagaccaga ccgccgccgt ctgatcgtcg gacgcggtgg ccacgcgcga ctcgtccggg
     1981 ctgaacgacg catgattgac ggcgccgtcg tgccgcagga cgatgcgttt ggcgccgctc
     2041 tcgacgttcc agattgccgc cgtggaatcc tgagaggccg tgaccgcggt tgtgccgctg
     2101 gcgccgaatt ccgccgacat cacccagtcc tcgtgcgtgc cgaacaccga cgggtcgggg
     2161 cgatcgagcg gccacagggt gaccgttccg tcgttcgatg ccaacaagag gctctttccg
     2221 tcagggctga aactggccgc ccgcgacggc accggcagcg gtcggtcctg cagcaggtct
     2281 ccggtgctcg cgtcccacac ccggatgctg ccatcgccgc cggtcacgat gcgcgtcgcg
     2341 tccggtgaaa actccgcgga caacacgctg ctctcgtgct cgagcgtcag cgtgagagcc
     2401 gcgccgccct cgaccggctc gagtcgatcc ctcgcccacg cgatcaccgg cacgatcggg
     2461 ctcggcgggg tgaacgccga ggcctcgcca aggtagaaca gtgccgccgc cggatcgctg
     2521 tcgaaaggca gattggcggc tggccaccgc agattccatc ccagctcgcc gcacatggcc
     2581 ctgagctgtt cggggatgtc attcagtgag gagggatcga cgccctccat ccagacgtcc
     2641 agcgtcaccc gggtctcgcc gaagattggc tgcacggtca gacgttgtcc gctcgcggtc
     2701 gtgaaacggc acaccgatgc cggcgtcggg tcggcaatga cctgccgcgg aagcggttcg
     2761 aagtcgatcg accgtgtctg ccagccttcg ggcgtcggcg actggatggc aattgccagc
     2821 ggcgccgtgt tcggaacgtg aaagatatga gcgccggccg ctgggggaac ggtcaactcg
     2881 atgccatcgg tacggcgctg gatgccgact gcgacggtgt tgagcgccca atcgatcgac
     2941 ggccgcccgc tcgcatcgcg cgcgacgcgg ggcacgccct cgagcaccgc ggtggcgtcg
     3001 cgtgcgcgta cccgttccgc cagcgcaatc gcggcggagg attgtcgcgc ggctgcgggg
     3061 agggcgggca ggctgcggac gatccactcg gtgagagcgc cgtccgagtc ttcggagagc
     3121 cccttcatca gcgtgccgag gccttcctgc atcgactggc gatgcgacgc gctcccgtag
     3181 gcggctgcgg cgagcgcgtg gtatgtcgtg gcttcctgca cgttgatcac cggaggcacg
     3241 ccagcggcgt ctgccggccg atggcagttg acgatgaccc gccacgccag ctccagcgtc
     3301 tggcgatgct cggggagcgt cagctgtcgg cgcaattcgt cgcggcattc aggaatcagc
     3361 gagaccccca gcatgtcgcg atcggcgacc agcgtgctga accacaggtt ggcctcgaga
     3421 ccggcatcgc tgtcgggaaa cagctccagc ctcatgtggc gcaccagcgt cgtttcaatc
     3481 cgcgtggcga ccgaaagcgc ccgggccagg tcgattacca gcggatcgag tgcccgctgc
     3541 gcggctagaa gggcagcgac gtcgacggcc atcaggttcg cgcccgcacg cttcggagca
     3601 ccgtcgccac gttggtgcgt gcgtcccacg gaacgacgtc gaacaccttg cgaagcgccg
     3661 gcggacacga atccggatga cgcggcagga tggcaaccgt atggcatccc gccgcgcgac
     3721 aacgtgcggc gaaaaccagc cattcggccg gcagggcgcc ccggatctcg ccctgatgct
     3781 gcgccaggcc caggtcgctg atgagcagga tgcgcgtgcc cgccgacggg gcctgccacg
     3841 gcgcacggat gcgcgtgcgg agccacagca cgtccccggc cagcggcgtg tccacgaagg
     3901 ccgccgtggc gaactgatcc ttcccgatcg tggcgcgcag gctgcgcagg atgtcgtcga
     3961 cgtcgcgggt gaacggcatc atcgactcgc tgcgatcgac cagaacgtgg accccgcgtc
     4021 gcagggtcgc tcgcggtcgg agcggcagcc gtaccaacgg ccgcagacgc gtgaccgcat
     4081 cgatcaggcg cggttcgtcg acggcgcctt cggcgatcgg caccgcgcag atgctcgaca
     4141 tcaggtgcct cgtccagtgg gggcggaaga gcgggagatg cgcaggcttg aacacggtgt
     4201 gggcggcctg ctcggccagc agcgcgggcg cgttggtggc ccacgatccc tcgtcggccg
     4261 cccccgcaat ctgatcctgg acggcgctcg acggaatcac gctcgccgcg acagcggcgc
     4321 gcggagccga caccgaatcc ttcccgtgcg cgccgacgcc ggtatcgtca gagcccatgt
     4381 tcgagcccgc accagaggct ggtgtttcgg tcgatggacg gaagcgagac gcgtcgaatc
     4441 ccaacgcttc cgccgccgcc gcccgatctt cgggcgtctc cagctgcagc gttcgccagg
     4501 cctcgagcac atctgaaatc cagatgtcgc cgcgattgcg cgcgttcacg tcgatcatgc
     4561 ggcggggtc
//
LOCUS       DATLCO010000295         4444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2086430, whole genome shotgun sequence.
ACCESSION   DATLCO010000295 DATLCO010000000
VERSION     DATLCO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086430"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VMZ33_06765"
     CDS             <1..651
                     /locus_tag="VMZ33_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HUR16969.1"
                     /translation="NQYHEPSRWLNEGVAVYLSEGYSNSFRGVVEVSALTGQLIPLQG
                     LAGLFPAGEGFFLAYGESVAAVDYFVNAYSEETLWNLVRSYADGLSDDEAFTAATGGD
                     VAAFNAAWMESLNTEVREPVGPQPAPAGPLPPGWSADGQPTAPASLPSSATQDPGTTP
                     RPGQIPPSDSGHDQSGSFLPALVVGVAAVVAVLVLFVVVMRRPQQKPPPLGPPGWD"
     gene            657..860
                     /locus_tag="VMZ33_06770"
     CDS             657..860
                     /locus_tag="VMZ33_06770"
                     /inference="COORDINATES: protein motif:HMM:NF017879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HUR16970.1"
                     /translation="MPPDPVLQVAIGNRLMLAKPHPCGSREWQVDRVGADIGLVCFGC
                     GRRVMLERRELERRFRGFVEPST"
     gene            857..2371
                     /locus_tag="VMZ33_06775"
     CDS             857..2371
                     /locus_tag="VMZ33_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16971.1"
                     /translation="MNAATHPAGSAQSEQTLNVLRNRRFLALWVAQVVTQVGGNMVLY
                     GLTVQVFGLTRSSTSVSLLILSFLVPAVIFGAVAGVYVDRIDRRLILVATNVLRAAFF
                     LAILFVINDIGLILLLTALISTLTTFFGPAEAAMIPVVVPRKQLLAANSLHVLTLQAS
                     FFLGFALLGPLVVNLAGQQVLLIAVGASYLVGAALCWFLPKYNPSRQDVQPDGGHALG
                     EAAEALGDAGTAVASTFSQLLDGLRYIRQNRTVFWPLTYLAVTASLIGVLGVLGPGFA
                     TQVLGLSEKDFVVVVLPLGVGLVMGIFALNIYGKYFSRRRGIEAGLVGLGVTLLALSV
                     AQGFTTRFSLGPLISLLAVVIVVAFFAGVAYAFVAVPAQTQLQEELPANIRGRVFGVL
                     NMLVSVASFVPIIVVGPIADAVGTSLVIQGSSVVVLLVAAGSILRGHSTADDTGGGGL
                     VDVADPVSITTSRSLTSPQTVDYMREDHDHGGAEKASPVVPGQPGPAREDADRS"
     gene            2368..3321
                     /locus_tag="VMZ33_06780"
     CDS             2368..3321
                     /locus_tag="VMZ33_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HUR16972.1"
                     /translation="MKRVALVFTGGTISMRPDAAAGGLRPAMSGAEILARVDGLDRIA
                     EVEVVDWGLVPASHMSMATILELAALMGELLGRPDIDGVVLVQGTDTIEETAFAFDLL
                     LPPDQPVVVTGAMRDTSAADYDGPRNILEAVGHAALDAAQDRSGVTVVLGGVAVGAEA
                     VVKAHATAMDAFRRREGAAEHPWKRLPRLPGPREAVDDVHLIVATTGMHGTLVRGVAG
                     GRPRGLVIAATGSGNTHPDLLAAATELMGSGTIVALTTRTAGGMVTPGYAFPGGGATW
                     LRAGALMSRLDGLKTRVALALALAAGLGREEIAELIGPAAA"
     gene            3318..>4444
                     /locus_tag="VMZ33_06785"
     CDS             3318..>4444
                     /locus_tag="VMZ33_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR16973.1"
                     /translation="MTATQADLIITGRIATLSGDSGFGWVEALAIGSDRVIAAGRRHD
                     VEPLLGPGTRTWRLPGDQVVVPGITDAHLHLMTMVVAGQQIDLNGADLDGALARIAQR
                     HLEMAAQGDNDSWILGHGWQLDLVGGWPTAADLDRAASGRPIALWTHDHHSRWLSSRA
                     LELAQLQDMPRNGLGSLVRRDEHGRATGILHEGGCMLVDAAIPPPTQDQLRAILKHVS
                     ADLLALGLTGVHDPGELTGDTDVQRGPVFYTALAEARRLPLRVHSSVRSLQLDRAIEL
                     GFRSGQETGRYRMGWLKLFADGSMGSRSAALLAPYDDAATNPSTGGPTGMVVTDAEAA
                     ETRHVSGVDELRVLDSALEWRRRRQSLKHVERHPYGGIAQSV"
BASE COUNT          643 a         1481 c         1512 g          808 t
ORIGIN      
        1 aaccagtacc acgagccctc gcgatggctg aatgagggtg tcgccgtcta cctctcggag
       61 ggttacagca actcgttccg gggcgtggtc gaggtttcgg cgctcaccgg gcagctcatc
      121 ccgctgcagg gcctggccgg cctcttccct gcgggcgaag ggttcttcct ggcctatggt
      181 gagagcgtcg cggcggttga ctacttcgtg aacgcctact cggaggagac tctctggaac
      241 ctcgtgcgca gctacgcgga tggcctgtca gacgacgagg cattcacggc cgcgaccggc
      301 ggcgatgtgg cagcgttcaa cgcggcatgg atggagtcgc tcaacacgga agtccgcgag
      361 ccggtcgggc ctcagccggc cccggcgggg ccactccccc ctggttggag cgccgacgga
      421 cagccgacgg ctccggcttc gctgccatcc tcagccacgc aggatccagg cacgactccg
      481 cggccaggtc agatcccacc gagtgatagc ggccatgatc agagcggcag cttcctgccg
      541 gcgttggtgg ttggtgtggc cgcggttgtc gccgttctcg tgctattcgt agtggtgatg
      601 cgccgaccgc agcagaagcc accaccgctt ggtccaccgg gctgggattg atcgacgtgc
      661 ctcccgaccc cgttcttcag gtcgccattg gcaatcggct catgcttgcg aagccgcacc
      721 cgtgtggatc gcgagagtgg caggtcgacc gcgtcggcgc ggatatcggc cttgtgtgct
      781 ttggttgtgg ccgacgcgtg atgctcgagc gtcgtgagct cgaacgccgc ttcagaggtt
      841 tcgtggagcc ctcaacatga acgcagcaac ccaccccgcc gggtcggcgc agtctgaaca
      901 gacgctcaac gtcctgcgca accgtcgctt ccttgcactg tgggtggcgc aggtcgtcac
      961 ccaggtcggc ggcaacatgg tgctgtacgg cctgaccgtg caggtgttcg gactgacgcg
     1021 ctcatcgacg tcggtgagcc tcctgatcct gagcttcctc gtcccggctg tgatcttcgg
     1081 cgccgtcgca ggcgtgtacg tcgaccgcat tgatcggcga ctgattcttg tcgccaccaa
     1141 cgtgctgcga gcggcgtttt tcctggccat cctgttcgtg atcaacgaca tcggcttgat
     1201 cttgctgctg accgcgctga tctcaacgct aacgacgttc ttcggcccgg ctgaggcggc
     1261 gatgatcccc gtcgtcgtgc cgcgcaagca gctgctggcg gcgaattcgc tgcatgtgct
     1321 gactctccag gcgtcgttct tcctggggtt tgccttactc ggcccactgg tcgtcaacct
     1381 tgccgggcaa caggttctgc tcatcgcagt cggcgcgagc tacctcgtcg gcgccgcgct
     1441 gtgctggttc ctgccgaagt acaacccgag caggcaggac gtgcagcccg atggcggcca
     1501 cgccctcggt gaggccgccg aggcccttgg cgatgccggc accgcggtgg cgtcgacgtt
     1561 cagccagctt ctcgacggcc tgcgctacat ccgccagaac cgaaccgttt tctggccgct
     1621 cacctatctg gctgtcacgg catccctgat cggtgttctg ggggtgctgg ggccggggtt
     1681 cgcaacgcaa gtgctcgggt tgtcggagaa ggacttcgtt gtcgtggtgc tgccactcgg
     1741 cgtcggcctg gtgatgggca tcttcgcgct caacatctac ggcaagtact tctcacgccg
     1801 ccgcggcatc gaggcggggc tggtcgggct cggcgtcacg ctgctggcgc tatctgtcgc
     1861 ccagggcttc actacgcgct tctcactcgg gccgttgatc tcgctactgg ccgtggtgat
     1921 cgtcgtcgcc ttcttcgccg gcgtcgccta cgctttcgtc gcggttccgg cgcaaaccca
     1981 actccaggag gagctgccgg ccaacattcg tggccgcgtc ttcggcgtcc tgaacatgct
     2041 cgtgagcgtg gccagcttcg ttccgatcat cgtcgtcggg cccattgccg acgccgttgg
     2101 cacatcgctg gttatccagg gctcgtcggt ggttgtgctg ctcgtggctg caggctccat
     2161 cctgcgtggg cactctacgg cggatgacac cggcggcggc ggcctggttg acgtcgctga
     2221 tcccgtctca atcaccacgt cgcgctcgct gacctcgccc caaaccgtcg actacatgag
     2281 ggaggaccac gaccacggcg gcgcggagaa ggcgtcaccc gtcgttcccg ggcaacccgg
     2341 gcctgcgcgc gaggacgccg acaggagttg aagcgcgtcg cgctggtttt caccggtggg
     2401 acgatctcga tgcgacctga cgcggccgcc gggggccttc ggccggccat gtccggtgct
     2461 gagatcctgg cccgcgtcga tgggctcgac cggatcgcgg aggtagaggt ggtcgactgg
     2521 gggctcgtgc cggcgtcgca catgagcatg gccaccatcc tcgagctggc tgcgctcatg
     2581 ggcgagttgc tgggccggcc cgacattgat ggcgtcgtgc tcgtgcaggg aaccgacacg
     2641 atcgaggaaa cggcgtttgc tttcgacctg ctcctaccgc ccgatcagcc ggtggtggtg
     2701 accggcgcga tgcgagatac ctcagccgcg gactacgacg ggccgcgcaa tatcctcgag
     2761 gctgtcggtc acgctgcgct cgatgccgcg caggacagga gcggcgtgac cgttgtgctg
     2821 ggcggtgtcg ccgtcggggc tgaggcggtt gtaaaggccc atgccaccgc gatggacgca
     2881 ttccgccgtc gcgagggcgc ggccgagcac ccgtggaagc gcctccctcg gctaccgggt
     2941 cctcgggaag cggtagacga cgtgcatctc atcgttgcca cgaccgggat gcacggcacg
     3001 ctcgtgcgcg gcgtcgcggg cggccgaccg cgcggcctcg tgatcgcggc gacgggttcg
     3061 ggcaataccc atccagatct gctcgccgcc gcgaccgagc tcatggggag tggcacgatc
     3121 gtggccctga ccacgcggac agctggtggc atggtgacgc cgggctacgc gtttcccggt
     3181 gggggagcga cgtggctgag ggcaggggca ctgatgtcgc gcctcgacgg tttgaagacg
     3241 cgtgtagccc tggcgctggc cctggcagcg ggcctgggtc gtgaagagat cgccgaactc
     3301 atcgggccgg ctgcagcatg actgcgaccc aggcagattt gatcatcacc gggcgtatcg
     3361 ccacgctctc gggggactca gggttcggct gggtggaggc gctggcaatt ggctccgatc
     3421 gcgtgatcgc agccgggcgt cgccacgatg tcgagccact cctcggcccg ggcacgcgga
     3481 catggcggct gccgggcgac caggttgtcg tgcccggcat aaccgacgcg catctccacc
     3541 tgatgacgat ggttgtggcc gggcagcaga tcgacctgaa tggtgcggac ctggatgggg
     3601 cgctggccag gatcgcccag cgccaccttg agatggccgc gcagggcgac aacgactcgt
     3661 ggattctcgg ccacggctgg cagctcgacc tcgtcggcgg ctggccgacg gcggccgacc
     3721 tcgatcgcgc cgcttccggc cgacccatcg ccctctggac gcacgaccat cactcgcgct
     3781 ggctgagcag ccgtgccctg gagctggcgc agttacagga catgccacgc aacggtcttg
     3841 gatccctggt ccgtcgtgac gaacatggcc gagcgaccgg catcctgcat gagggcggct
     3901 gcatgctcgt cgacgcagcc atacctccgc cgacacagga ccagctgcgc gccatcctca
     3961 agcatgtctc tgctgacctg ctcgcactgg ggctcaccgg cgtccacgac cccggcgagc
     4021 tgaccggcga caccgacgtc cagcgtgggc ccgtcttcta cacggccctg gccgaggcca
     4081 ggcggctccc acttcgagtc cactcctcgg ttcgctccct gcagctcgac cgcgccatcg
     4141 agctcggctt ccggagcgga caggaaaccg gccgctaccg gatgggctgg ctaaagcttt
     4201 tcgctgacgg aagcatgggt tcgcgcagcg cggcattgct cgcgccgtac gacgacgctg
     4261 ccacgaatcc gtcgaccggc ggaccgaccg gaatggtcgt gaccgacgcc gaggcggccg
     4321 aaacgcggca cgtcagcggg gtcgacgagc tgcgcgtgct cgattcggcg ctggagtggc
     4381 ggcgtcggcg gcagtccctc aaacacgtcg agcgccatcc gtacggcggc atcgcccaga
     4441 gcgt
//
LOCUS       DATLCO010000296         3018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6260486, whole genome shotgun sequence.
ACCESSION   DATLCO010000296 DATLCO010000000
VERSION     DATLCO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6260486"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VMZ33_06790"
     CDS             <1..573
                     /locus_tag="VMZ33_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16974.1"
                     /translation="AVSLAVIVVILSTTSASAATATVTMTNYAFTAKAQTVRLGNSVT
                     WRNTSTKTHTATPSVNWSWGGVSVAKGATSANVSPTQAGGFAYYCSIHPTLMKGNIKV
                     PITVNPAGGNTATSFNLTLGTVKAPGVLVHDVYIRRNGGAWILRVETAEPSVSISFTQ
                     TGTWGIRTRLRYQLGGATSGFSPISTVVVF"
     gene            complement(570..1385)
                     /locus_tag="VMZ33_06795"
     CDS             complement(570..1385)
                     /locus_tag="VMZ33_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16975.1"
                     /translation="MPNQYLELVIDFEGELIAFGHEINNCSDVGPGMIWRSSDGGTTW
                     QHAVTLSAAGPGHWMDTTTTGGRVLATTAGTSGVRVWLSDDGLEWRGPSGNGPARRYL
                     GGLNEVVVSSNATYEEEVTVMTSSDRGESWEEVALELRGEFRPSFAANNGLLVGVFSE
                     FERAWPMTTQDGLTWTVAEEPLQTDVDKVVAVPGGFLSLSNRGSTYLSADGLDWFAGP
                     PQRMLTDDRGIRGAVADGVGVLLFTMDFRNARQYDEHLWFAPIGAFDPFWSTD"
     gene            complement(1860..>3018)
                     /locus_tag="VMZ33_06800"
     CDS             complement(1860..>3018)
                     /locus_tag="VMZ33_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16976.1"
                     /translation="LAALVMQCLEKDPDNRPLSAREVADRLARIGERLVAQGAGLAAV
                     VPSAAAAAASPDEDTVVIPAVAAAAAGPWSGPPAASLPVAAVGATVAGDPELGVAPGR
                     PAYERDDHRERSSPWSTVGFVVIALILLALAAVAAFAFLGSRGGGVADATATPTSRFT
                     PTLRATPSSAIVVPPVGTPLPELPTPTPEFISPEPVTPEPPVITPEPPVVTPEPPVVT
                     PQPPVITPQPPVVTPGPPAPQTVRVRIPDESFSGDFPDETTYHGRSASWVYGQGTPYN
                     SMTASFATPGVDPSGPATLQLVGLDGENELKNATRIAINGVTIYEGPNPLPNDFGTGG
                     PGNWGTVTFSIPAGVLGTSNTLTVTNLEDNDCTNCPKYVMIDRAVVDYTAL"
BASE COUNT          523 a          993 c          984 g          518 t
ORIGIN      
        1 gccgtgtccc ttgcggtcat cgtcgtcatc ctctcgacca cgagtgcgtc ggccgcgacc
       61 gcgacggtca ccatgaccaa ctacgccttc accgccaagg ctcaaacggt caggctcggc
      121 aactccgtga cctggcgcaa cacgtcgacc aagacgcaca ccgctacgcc gagcgtcaac
      181 tggtcatggg gcggagtctc cgttgccaaa ggcgcgacct cggccaacgt ctcgcccacc
      241 caggcaggcg gctttgccta ctactgttcg atccacccaa cgctgatgaa gggcaacatc
      301 aaggtgccga tcaccgttaa ccccgcgggt ggcaataccg cgacgtcctt caacctcacg
      361 ctggggacgg tcaaggctcc cggcgtcctg gtgcacgacg tctacatccg ccgcaacggt
      421 ggcgcgtgga tcctccgcgt tgagactgcc gagcccagcg tctccatctc cttcacgcag
      481 acggggacgt ggggtatccg cactcgcctt cgctaccagt tgggcggcgc gacgagcggc
      541 ttcagcccga tctcaacggt ggtcgtcttc tagtcggtcg accagaacgg gtcgaacgca
      601 ccgatcggcg cgaaccagag atgctcgtca tattgacgtg cgtttcggaa gtccatcgtg
      661 aacagcaaca cgccgactcc gtcggccacg gccccgcgga tccctcgatc gtcggtaagc
      721 atgcgttgcg gcggacctgc gaaccagtcg aggccgtcag cagacaagta cgtcgaaccg
      781 cgattactca gtgacaggaa tccgccgggc acggccacga ccttgtcgac gtcggtctgc
      841 agcggctcct ctgcaactgt ccacgtgagt ccatcttgcg tcgtcatggg ccacgctcgc
      901 tcaaactcgc tgaacacgcc taccagcaag ccgttattcg cagcgaagct tggacggaac
      961 tcgccccgga gctcgagcgc cacctcttcc catgactcgc cgcggtcgga ggaggtcatc
     1021 acggtcactt cctcttcgta tgtggcgttt gaggacacga cgacttcgtt gagtcctccg
     1081 agatagcgac gagcaggccc gttgcctgac ggaccgcgcc actcgaggcc gtcgtccgat
     1141 aaccagacgc gaacgccaga cgtgcccgcg gtggtggcga gtacgcggcc gcctgtcgtc
     1201 gtcgtgtcca tccaatgacc ggggccggcc gcgctcaatg tgacggcatg ctgccaggtc
     1261 gtgccaccgt cgctggagcg ccaaatcatt ccggggccga catctgagca gttgttgatc
     1321 tcgtggccga aggcgatcag ctccccttcg aagtcgatca cgagctcgag atactgattc
     1381 ggcatttccg tttgctgcag tgtccactcc cggccactcg gtgatgtcca cacgcgccct
     1441 tcagacgagc agccgttgtc caccaggctg agaccgaccg ccacataacc tggaccgcca
     1501 acgccgatcc cctcaatctg gtcgtggggc ccgaggaagt cgtcgacctg ccggaatggc
     1561 gatcggccct caagaatcga ggccgtgggc gaggttgtag gagtcgccgt cggggtcgaa
     1621 gtcggcggtg gcccttttgg agtcaaaggt ggcaagtgcg tgggggacag ggtcgactga
     1681 ctcggcagag cagtggggat cctgctcgcg ggcaatggca cggagggctg ggcaatcggc
     1741 gtcgctgacg gagtaaccgt cgaggtgcag cccgtgaacg ccaggatcca aacgatcgcc
     1801 agccgctgaa acctcgtcac gtcaccagtg tgacgaagcg tccacgcgtg gcgttgcggt
     1861 tagagggcgg tgtagtccac tacggcgcgg tcgatcatca cgtacttcgg acagttcgtg
     1921 cagtcgttgt cttcgaggtt ggtcaccgtg agggtgttgc tcgtccccag cactcccgcg
     1981 gggatgctga aagtgacagt gccccagttg ccgggcccgc ccgtcccgaa atcgttcggc
     2041 aacggattag ggccctcgta gatcgtcacg ccattgatcg cgatccgggt cgcattcttg
     2101 agctcgttct cgccatcgag gccgacgagc tgcagcgtgg cgggcccgct cggatcgacg
     2161 ccgggggtag cgaacgatgc tgtcatcgag ttgtacggcg tcccctgccc gtagacccag
     2221 gatgcggagc ggccgtggta ggtcgtctcg tcggggaagt cgccggagaa tgattcatcg
     2281 gggatacgga cccgcactgt ttggggcgcc ggtggcccag gggtgacgac cggcggctgc
     2341 ggcgtgatca ccggcggctg cggagtcacc acgggcggtt caggggtcac gaccggtggc
     2401 tccggcgtaa tgaccggcgg ctcgggcgtc accggctccg ggcttatgaa ttcgggtgtt
     2461 ggagtcggaa gctccggcaa cggcgttccc accggcggta cgacgatcgc cgagcttggc
     2521 gtcgcgcgga gggtgggcgt gaagcgggag gttggcgtgg ccgtcgcatc agcgacaccg
     2581 ccgcctcgcg accccaggaa cgcgaacgcg gccacggccg ctaatgccag aagtatcagg
     2641 gcgatcacca cgaatccgac cgtcgaccac gggctcgagc gttctcgatg atcgtcgcgc
     2701 tcatacgcgg gacgtcccgg agcgacgccc agctcggggt cgcctgcgac agtggctcct
     2761 accgcggcca cgggcagaga cgcagccggc ggtcctgacc atggcccggc agccgcagcc
     2821 gccacggccg ggatgacgac cgtgtcttca tctggcgagg ccgcggcggc ggcagccgaa
     2881 ggcaccacag cggcaaggcc cgcgccttgc gccacgagcc gctcaccaat tcttgcgagt
     2941 cgatcggcga cctcgcgcgc gctcaacggt cggttgtcgg gatccttctc gaggcattgc
     3001 atgaccaagg ccgccagt
//
LOCUS       DATLCO010000297         5615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9997099, whole genome shotgun sequence.
ACCESSION   DATLCO010000297 DATLCO010000000
VERSION     DATLCO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9997099"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VMZ33_06805"
     CDS             <1..232
                     /locus_tag="VMZ33_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR16977.1"
                     /translation="NGGYTNAEGSMSKLKAGRVAVWATERAIQILGGYGYTREYPVER
                     WHRDAKIFDIFEGAEQIQQLVISRAISGLRVE"
     gene            complement(218..616)
                     /locus_tag="VMZ33_06810"
     CDS             complement(218..616)
                     /locus_tag="VMZ33_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR16978.1"
                     /translation="MDADGLRLRVLIVDDTPVIRAVVRRILERDGRFEVVGEACDGLE
                     AVDMTEGLQPDLVLLDLAMPRMDGLQALPEIITNAPSVTVVVLSSFSRDQMKDAALDA
                     GALGYVEKDRIAITLVPQIVSVCTSLLLDA"
     gene            complement(621..1439)
                     /locus_tag="VMZ33_06815"
     CDS             complement(621..1439)
                     /locus_tag="VMZ33_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16979.1"
                     /translation="MTASRWLPEGRGLPYDTWSLRHNAIVWILWCHTIGIFGFGLATG
                     HAVVHSLLEASVLVPYALVARSHRPSQLVRTIATTVGLLTASAILVHLAGGSIEMHFH
                     FFVMIGVITLYQDWRPFMVGIIFVAAHHGVMGTVDAQAVFNHPSAWNNPWKWAVIHAL
                     FILGASAAYITGWRYTELERHRAEDYGAQLAESVLFQREALEINDNIIQGLVAVEAAM
                     DFDDETLARDALNATIVSAREIVSNLLRRAKADGRLTPGDLRRDLPGFRTNASV"
     gene            complement(1494..2051)
                     /locus_tag="VMZ33_06820"
     CDS             complement(1494..2051)
                     /locus_tag="VMZ33_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16980.1"
                     /translation="MTLDVDTIVERLKSANQEASAQLAVRDVLHELIASNQLDAALPE
                     VAQGGITTLYNAPDLTVLRVAWTPGMRLNPHNHLMWAAIGLYGGVEDNAFYRRTPQGL
                     VESGGKEVSAGEVLLLGDDAIHAVANSRREYAVAMHVYGGDFFGDGRSEWDYETLTEG
                     PRDIEGTMRLFREANERWVKEKPTN"
     gene            complement(2139..2600)
                     /locus_tag="VMZ33_06825"
     CDS             complement(2139..2600)
                     /locus_tag="VMZ33_06825"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR16981.1"
                     /translation="MTARLFVICGLPGSGKTTLARQLEAERAAVRFCPDDWFVFLQLD
                     IWDQELRGRIEALQWGLAQRILELGGEVVIEFGSWARSERDELREGARRLSADVELRY
                     LDPGFEELVRRVEARQGFDPPITRAHLEEWSRVIEVPTEAELALFDPPSGS"
     gene            complement(2597..2893)
                     /locus_tag="VMZ33_06830"
     CDS             complement(2597..2893)
                     /locus_tag="VMZ33_06830"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HUR16982.1"
                     /translation="MPNPDAAALEELEDLRASIDNLDAALIHLLAERFKCTQRVGRLK
                     AAHGMPAADPAREARQVGRLRALAEEAQLDPEFAAAVLDFIIAEVIRHHEALQE"
     gene            2927..3865
                     /locus_tag="VMZ33_06835"
     CDS             2927..3865
                     /locus_tag="VMZ33_06835"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HUR16983.1"
                     /translation="MPFDNRVTQMLGVEIPIVQAPMGWIARSQLASAVSNAGAMGIIE
                     TSSGELDNVKDEIRKMRDLTDKPFGVNIAQAFVRDPAIAEFVIDQGVKFVTTSAGSPT
                     KYTSVLKEGGLTVFHVVPNLTGAMKAVDAGVDGLVVEGGEGGGFKSPRPIASMVLLPL
                     IRSKTNVPIIAAGGFIDGPTMAAALAMGAEGVQMGTRMVSSAESPVHNNWKQAIVDAG
                     EDDTVVLGRAGRMAMRALRTDRTTALEFNTDTEPMSAFGDAMKLYFGGDMEAGIALGG
                     QVMGRIEEVRPVGDVISETAADCLAIIEGLAKQYPR"
     gene            complement(3831..4655)
                     /locus_tag="VMZ33_06840"
     CDS             complement(3831..4655)
                     /locus_tag="VMZ33_06840"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR16984.1"
                     /translation="MTALPVLDAGPGSLLRQWRLHRRMSQLDLSNRAGVSTRHLSFVE
                     TGRAQPSRELLLHLGRALDIPMRDCNRLLLAAGFAPVFGEVPLDAPEQRDTLAAVERV
                     LNAHEPWPALVVDRYWNVVSANNAAMLFVEEVAPELLVPPVNVLRVCLHPEGLAPRIG
                     NLGTMAHHVLGNLQRQIDALGDPELAGLADELRGYLSGVDINDSDGGDGLSVLMELRR
                     GENTLRFVSLIATFGSAVDATTSELVIETFLPADDETAVALSTLALSAGTASPAPR"
     gene            4743..5411
                     /locus_tag="VMZ33_06845"
     CDS             4743..5411
                     /locus_tag="VMZ33_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HUR16985.1"
                     /translation="MQPLQSRKEIVEAFQEGLVRFSALVRPLTVEQLATPSRCEGWSA
                     GDVAGHYVGSITEIAAGEVEGQGTPAVTKRQVEARKGRSGAELADELDAAATAVPLLL
                     AAFPDDAWDAPLSGFDGTLAQGVEALLFDGAVHADDIDAALHLGHHPTQAELVAAVSH
                     VAFHLCAQNWGPATLALDGIPEVEIGDGGPRISGNPGTFVLAATGRGNPAEFGLDDTV
                     NIYR"
BASE COUNT          984 a         1777 c         1916 g          938 t
ORIGIN      
        1 caacggcggc tacaccaacg ccgaagggtc catgagcaag ctcaaagccg gacgggttgc
       61 ggtgtgggcg actgagcgcg ccatccaaat cctcggcggc tacggctaca cccgcgagta
      121 ccccgtcgag cgctggcacc gtgacgccaa gatctttgac atcttcgaag gcgcggagca
      181 gatccagcag ctcgtgatca gtcgggcgat ctcgggtcta cgcgtcgagt agcagcgagg
      241 tacagacgga aacgatctgg ggcacgagag tgatcgcgat gcgatccttc tccacgtagc
      301 ccaaggctcc cgcgtcaagt gctgcgtcct tcatctgatc gcggctgaag ctggaaagca
      361 cgaccacggt caccgacgga gcgttggtga tgatctccgg cagcgcttgg agcccatcca
      421 tgcgcggcat ggcgaggtcg agcagaacga ggtcgggttg caggccctcg gtcatgtcga
      481 ctgcctccaa gccgtcacac gcttcaccga cgacctcgaa gcggccgtcg cgttccagga
      541 tgcgacgcac cacggcgcgg atcaccgggg tgtcgtccac gatcaggacg cgtagacgca
      601 atccgtcggc gtccatggac ctagacgctc gcgttggtgc ggaagccggg caggtcacgt
      661 cgcaggtcgc caggagtcaa gcggccgtcg gccttggctc ggcgcagcag gttgctcacg
      721 atctcgcggg ccgacacgat cgtggcgttc agggcgtcgc gggccagcgt ctcgtcgtcg
      781 aagtccatcg cggcttcgac cgccacgagg ccttgaatga tgttgtcgtt gatctcgagc
      841 gcttcgcgct ggaacagcac gctctcggcg agctgcgcgc cgtagtcctc ggcgcggtga
      901 cgctccagct cggtatagcg ccagccggtg atgtacgcgg cgctcgcacc gaggatgaac
      961 agtgcatgga taacggccca cttccagggg ttgttccagg cggaggggtg attgaagacc
     1021 gcttgcgcgt ccacggttcc catgaccccg tggtgggcgg cgacgaagat gatgccgacc
     1081 atgaacggcc gccaatcctg atacagggtg atcacgccga tcatgacgaa gaagtggaag
     1141 tgcatctcga tcgagccgcc ggccaggtgc acgaggatgg ccgaggcggt caggaggccc
     1201 acggtggtcg cgatcgtgcg caccagctgc gaaggccggt gcgagcgggc gacgagggcg
     1261 taggggacaa ggaccgacgc ctcgaggagg ctgtggacta cggcgtggcc ggttgcgagg
     1321 ccaaagccga agattccgat cgtgtggcac cagaggatcc aaacgatcgc gttgtggcgc
     1381 agggaccatg tgtcataggg cagtcctctg ccttccggaa gccaacgaga tgcagtcacg
     1441 ggctttccat cggttgttgg gaacccgaag tccaggattt gacaatcagt tgatcaattt
     1501 gtgggttttt cttttaccca gcgctcgttg gcttcccgga acaaccgcat cgtgccttca
     1561 atgtcccgtg ggccctcggt gagcgtctcg tagtcccact ccgagcgacc gtcgccaaag
     1621 aagtcacccc cgtacacatg catcgccacc gcgtattcac ggcggctatt ggccacggcg
     1681 tggatggcgt cgtcacccag caggagaacc tcgccggcac tgacttcctt gccgccggac
     1741 tccacgagcc cctgcggcgt gcgccggtag aaggcgttgt cctccacgcc tccgtacagg
     1801 ccgatggcgg cccacatgag gtgattgtgc gggttgagcc tcatgccagg cgtccaggcg
     1861 acgcggagca ccgtgagatc cggcgcgttg taaagcgtgg tgatgccgcc ctgggcgact
     1921 tcgggcagcg cggcgtcgag ttggttcgag gcgatcagct cgtggagcac gtcgcgcacc
     1981 gccagctgcg ccgaggcctc ctggttggcc gacttgagcc gttcgacgat ggtgtctacg
     2041 tcgagtgtca tacgcccaga gtatgaacgt tgcctgtcaa gcgcccgccg cgcactggct
     2101 catcggccgc tccggcaatt ccctgagctt ccggaccgtc aacttcccga gggtgggtcg
     2161 aacagggcga gttctgcttc tgtcgggact tcgatgaccc gtgaccactc ttcgaggtgg
     2221 gcgcgggtga tgggcgggtc gaagccttgc cgggcctcga cgcgacggac gagttcctcg
     2281 aagcccgggt cgaggtagcg cagttctacg tcggcgctca atcggcgggc gccctcgcgc
     2341 agctcgtcgc gctccgagcg ggcccacgaa ccgaactcga tcacgacctc gccgccgagt
     2401 tcgaggatcc gctgcgccag gccccactgc aacgcctcga tgcgaccccg cagctcctgg
     2461 tcccaaatgt cgagctgcag gaagacgaac cagtcgtcgg ggcaaaagcg cactgccgcc
     2521 cgttcggcct ctagttggcg ggccaacgtg gtcttgcccg agccgggcag tccgcaaatg
     2581 acgaagaggc gcgccgtcac tcctggaggg cttcgtgatg gcggatgacc tcggcgatga
     2641 tgaagtcgag cacggcggcc gcgaactcgg ggtcgagctg cgcttcctct gccagtgccc
     2701 gcaggcgtcc gacctgccga gcctcccggg ccgggtcggc ggcgggcatg ccgtgagccg
     2761 ccttcagccg accgactcgc tgcgtgcact tgaaccgttc cgctagcagg tgaatgagcg
     2821 cggcgtcgag attgtcgatg ctggcgcgca agtcctcgag ctcttcgagc gcggctgcgt
     2881 ccgggttcgg catgcggaca gctttgcgga cgacaacaat gcctgcatgc cctttgacaa
     2941 ccgcgtcacg caaatgctcg gcgtcgagat cccgatcgtg caggcgccga tggggtggat
     3001 cgcgcgctcg cagctcgcct ctgcggtatc caacgccggc gccatgggga tcatcgagac
     3061 gagttcaggc gaactcgaca atgtcaaaga cgagatccgc aagatgcgcg acctcaccga
     3121 caagcccttc ggcgtcaaca ttgcccaggc gttcgtgcgc gatcccgcga tcgccgagtt
     3181 tgtgatcgat cagggcgtga agttcgtcac cacgtccgca ggtagcccga cgaagtacac
     3241 gtcggtactg aaggaaggcg ggctcacggt cttccacgtc gtgcccaacc tcacgggcgc
     3301 gatgaaggcg gtcgacgcgg gagtggacgg gctcgtggtc gaaggcggtg agggcggcgg
     3361 ctttaagagc ccgcggccga tcgcttcgat ggtgttgctg ccgctcattc ggtcgaagac
     3421 gaacgtgccg atcatcgcgg cgggtggatt catcgacgga cccaccatgg ccgccgcgct
     3481 ggccatgggt gccgagggcg ttcagatggg cacccgcatg gtgagcagcg ccgagtcgcc
     3541 tgtccacaac aactggaaac aggcgatcgt cgacgccggc gaagacgaca cggtcgtact
     3601 cggccgagcg ggtcggatgg cgatgcgggc gctgcgaacc gatcgcacca ctgccctcga
     3661 gttcaacacc gacaccgaac cgatgagcgc gttcggcgac gccatgaagc tctacttcgg
     3721 cggcgacatg gaagcgggca tcgcactcgg cggccaggtg atgggccgca ttgaagaggt
     3781 gcgaccggtg ggagacgtga ttagtgagac cgcggccgac tgcctcgcca tcatcgaggg
     3841 gctggcgaag cagtacccgc gctgagagcg agtgtgctca acgccaccgc ggtttcgtcg
     3901 tcggccggca agaaggtctc gatgaccagc tccgaagtcg tcgcgtcgac agcgctgccg
     3961 aaggttgcga tcaggctgac gaatcgcaac gtgttctcgc cgcgtcgcaa ctccatcaac
     4021 accgataacc cgtcgccacc gtcggagtcg ttgatgtcga cacccgacaa gtagccgcgc
     4081 agctcatcgg cgagacccgc aagttcggga tcaccgagcg cgtcgatctg gcgttgcagg
     4141 ttgccgagga cgtggtgcgc catcgtgcct aagttgccga tgcgcggcgc gagtccctcg
     4201 ggatgcaggc atacgcgcag cacgttgacg ggcggaacca gcagttccgg cgcgacctct
     4261 tcgacgaaca gcatcgccgc gttgttcgcg ctcacgacgt tccagtaacg atcgacgacg
     4321 agcgcaggcc agggctcgtg ggcgttgagc acgcgctcga ccgcggccag cgtgtcgcgc
     4381 tgctccggcg cgtcgagcgg cacctcgccg aacacgggag cgaatccagc ggcgagcagg
     4441 agtcggttgc agtcacgcat cggtatgtcc agcgcgcgac cgagatgcag gagcagttcg
     4501 cggctcggct gggcgcgccc ggtctccacg aagctgaggt ggcgcgtcga aactccggcc
     4561 cggttcgaca agtcgagctg gctcatgcgt cggtggagtc gccactggcg aagcagcgag
     4621 cccggcccgg cgtcgaggac gggaagcgcg gtcatgacct ggaaagtaat gaacgctcgc
     4681 gagggcgacc atgacctgtc aggtaattgg cgcgatgacc gtcgaggcgg aaggtggtgg
     4741 gtatgcaacc actccagtca cgcaaagaaa tcgtggaggc gttccaagaa ggtctggtgc
     4801 gcttctcggc cctcgtacgc cctctgaccg tggagcagct ggccactccc agccgctgcg
     4861 aagggtggag cgccggcgat gtggccggcc actatgtcgg ttcgattacc gagatcgcag
     4921 ccggtgaagt cgaaggccag ggcacgccgg cggtgaccaa gcggcaggtc gaggcgcgca
     4981 agggccgctc cggcgccgag ctagcggacg agctggacgc ggccgcgacc gccgtaccgc
     5041 tcctgctcgc tgccttcccc gacgacgcgt gggacgcacc gctctccgga ttcgacggca
     5101 cgctggcgca gggcgtcgag gcactgctct tcgacggtgc ggttcacgcc gacgacatcg
     5161 acgcggcgct gcacctgggc caccacccga cgcaggcgga gctggtcgcg gcggtgtcgc
     5221 acgtggcgtt ccacctttgt gcccagaatt ggggtccggc gaccctcgcg ctcgacggca
     5281 ttccagaggt cgagatcggt gacggcgggc cgcgcatcag cggcaatccc ggcacgttcg
     5341 ttctcgccgc cacaggccgc ggcaacccgg ccgaattcgg cctcgacgac acggtcaaca
     5401 tttatcggta actcgctgtc ttttggtcac ttcttgccgg gtccttagga tgcggccatg
     5461 agtgatctgg gtttcgacgg caaagttgcg atcatcaccg gcgcaggcgg cggtcttggc
     5521 cggtcgcacg ctctggagtt ggctaagcgt ggcgctctgg tcgtggtcaa cgacctcggg
     5581 ggttcgaccg atggcaccgg agcgtcagag acagc
//
LOCUS       DATLCO010000298         2770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2103490, whole genome shotgun sequence.
ACCESSION   DATLCO010000298 DATLCO010000000
VERSION     DATLCO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2103490"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VMZ33_06850"
     CDS             complement(<1..530)
                     /locus_tag="VMZ33_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HUR16986.1"
                     /translation="MVDRRTFLMQAGSVATSALVLPSMQKRYKIGLELFTVNRDMNRD
                     PLASLKRVAEMGYEEVETYGLNPDALTYYGLPAADFAKRLQDHKLPSPSGHYDLQQFL
                     SGSPDDLNRYVDRCAEGARVLGQRYIVWPFIEPKLRTLETFKRVAGLLNAIGSRLEKS
                     GLTVAYHNNGGEFIPQG"
     gene            609..1430
                     /locus_tag="VMZ33_06855"
     CDS             609..1430
                     /locus_tag="VMZ33_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16987.1"
                     /translation="MAGQLRYYSGARSEQGAVAMKIRRLARALVIGLPLTAGATLLAI
                     GTGQTPVGPAAAGDPLIAGFKSTYAASVSDAVEIVTGRNGTMRSDMKLMAGTNMVGRA
                     VTSLARPAPAEQATAALSVKHSVEIIDEAKPGEVGVIVMEGTLDIAAMGNLMATAAVE
                     RGMAGMVLDGAIRDMWDIRRMGLTVYARAKSPRTAVGHYATVAKNIPVECGGVTVRSG
                     DIIVADEDGVVVVPQDRAADVLKEAQSIDARESGMYPFIRQFKSLQEAIKKFNRI"
     gene            1593..>2770
                     /locus_tag="VMZ33_06860"
     CDS             1593..>2770
                     /locus_tag="VMZ33_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR16988.1"
                     /translation="MVTYLDRTAIGTLAPGIRRDLGLSAIEMGWVFTAFQLAYGLFEV
                     PTGRWADRVGTRSVLARIVIWWSVMTMATAAAVSYATMLAIRFLFGAGEAGAWPSVAR
                     TFSRWIPQPERGRVQGIFFAGAHLVSGLTPAFIVGGGLLGPWPGLLTVMSWRGVFVTF
                     GVVGLVWVAIWLSWFRNDPSEHPSVSPSELALIVAHRKPVTEHGGGWAYWRTLIRSRN
                     MIALSVMYIPNCMIFYFCITWLPSYLLERHGFNVSGMALFAGLPLLVSMPGDLLGGWL
                     TDRLCSRYGLRVGRCGLGAIAYVGVGICLIAAASASAPVPAALLIAAATGLTMFTLGA
                     AWGTVMEIGGNHVGVVGGTMNSIGNLIAMLNPLIVAYSVSWFGSWDLPLYVMGVLFLV
                     G"
BASE COUNT          399 a          888 c          978 g          505 t
ORIGIN      
        1 ccctggggga tgaactcgcc gccgttgttg tggtacgcga ccgtgaggcc gctcttctcc
       61 agccgtgagc cgatggcgtt cagcaggccg gcgacccgtt tgaaggtctc gagggtccgg
      121 agcttcggct cgatgaacgg ccagacgatg tagcgctggc cgaggacgcg ggccccctcg
      181 gcacagcggt ccacgtagcg gttcaggtcg tcgggactgc cactcaggaa ctgctgcaga
      241 tcgtagtgcc cgctcgggct gggcagcttg tgatcctgga gccgcttggc gaagtccgcg
      301 gccggcaggc cgtagtaggt cagcgcgtcg ggattgaggc cgtaggtctc gacctcttca
      361 tagcccatct cggccacgcg tttgagggac gcgagcggat cccggttcat gtcccggttg
      421 accgtaaaga gctcgaggcc gatcttgtac cgcttctgca tggacggcag gaccagcgcg
      481 ctggtggcca ccgaaccggc ctgcatcaga aacgtgcggc ggtcgaccat ggaacctcca
      541 cgaggaaagc agcgtgtgct gcgcggtgtc ggatagggcc gccggacgac cgaggccgcc
      601 cggccagcgt ggccggccag ctacgttact atagcggcgc tcgaagcgaa caaggagcgg
      661 tggccatgaa gattcgtcgt cttgcacgtg cgctcgtgat cggtctgccg ctgacggccg
      721 gcgcgacgtt gctcgcgatt ggcacgggac agacaccggt cggcccagcc gccgccggcg
      781 acccgctgat cgccggcttc aagagcacct acgccgcgtc ggtgtcggac gcggtcgaga
      841 tcgtgaccgg caggaacggc acgatgcgct ccgacatgaa gctcatggcc ggcaccaaca
      901 tggtcggccg tgccgtgacc tccctcgccc ggccggcgcc cgcggagcag gcgacggcgg
      961 cgctgtccgt caagcactcg gtggagatca tcgacgaggc caagcccggc gaggtgggcg
     1021 tgatcgtcat ggaggggacg ctcgacatcg ccgcgatggg caacctcatg gccacggcgg
     1081 ccgtcgagcg cgggatggcc ggcatggtgc tggacggcgc gatccgcgac atgtgggaca
     1141 tccgtcgcat gggcctgacg gtctatgcgc gggccaagtc gccgcgcacc gcggtcggcc
     1201 actatgcgac cgtcgccaag aatattcccg tcgagtgcgg cggggtgacc gtgcgttcgg
     1261 gcgacatcat cgtcgcggac gaagatggcg tggtggtcgt gccgcaggac cgcgccgccg
     1321 acgtcctcaa ggaggcgcag tcgatcgacg cgcgtgagag cggcatgtat cctttcattc
     1381 gccaattcaa gtccctgcag gaagcaatca agaagttcaa ccgcatctag cggttcatcg
     1441 acgcgtgaag gaggccttca tgcgccagcg tcgtcgtgcc ggtcgggtcg gcggggttga
     1501 acgtgagaga ggttgctaga gtgccagtcc agaccaccca gacgacgtcc accgcccgct
     1561 accgcgttgt cgcgctcgcg atgacactcg ggatggtcac ctacctcgat cgcaccgcga
     1621 ttggcacgct cgcccccggt atccgccgcg atctcggcct gtcggcaatc gagatgggct
     1681 gggtgttcac cgcgttccag ctcgcctacg ggctcttcga ggtcccgacc ggacgctggg
     1741 cggatcgggt cggcacccgc tccgtcctcg cgcgcatcgt catctggtgg tcggtgatga
     1801 cgatggcgac cgcggcggcg gtgagttacg cgaccatgct cgcgatccgg ttcctgtttg
     1861 gggccggtga agcgggcgcc tggccgagcg tggcgcgcac tttctcacgg tggattccac
     1921 agccggagcg gggccgggtg caggggatct tcttcgccgg cgctcacctg gtctccgggc
     1981 tgaccccggc cttcatcgtc ggcggcgggc tgctcggccc gtggcccggc ctgctgaccg
     2041 tgatgtcgtg gcgcggtgtg ttcgtgacgt tcggcgtcgt tggcctcgtg tgggtcgcga
     2101 tctggctctc ctggttccgc aacgatccgt ccgagcatcc atcggtcagc ccgtcggagc
     2161 tggccctgat cgtggcccat cgcaaaccgg tcaccgagca cggtgggggc tgggcctact
     2221 ggcgcacgct gatccgcagc cggaacatga tcgcgctcag cgtcatgtac atcccgaact
     2281 gcatgatctt ctacttctgc atcacctggc tgccgtcata cctgttggag cggcacggct
     2341 tcaacgtctc gggcatggcg ctcttcgccg ggctgccgct gctggtgagc atgcccggcg
     2401 acttgctggg aggctggctc actgatcgtt tgtgcagccg ctacgggctc cgggtcggac
     2461 gctgcggact gggcgcgatt gcctatgtcg gggtcgggat ctgcctcatc gcggcggcct
     2521 cggcctcggc gccggtgccc gcggcgctgc tgatcgccgc cgccacgggt ctgacgatgt
     2581 tcacgctcgg cgcggcgtgg gggacggtca tggagatcgg cggcaaccac gtgggtgtgg
     2641 tcggcggcac gatgaactcg atcggcaacc tgattgcgat gctcaatccg ctgatcgtgg
     2701 cctactcggt ctcctggttt ggcagctggg atctgccgtt gtacgtgatg ggcgtcctct
     2761 ttcttgtcgg
//
LOCUS       DATLCO010000299         4287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3303536, whole genome shotgun sequence.
ACCESSION   DATLCO010000299 DATLCO010000000
VERSION     DATLCO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3303536"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VMZ33_06865"
     CDS             complement(<1..285)
                     /locus_tag="VMZ33_06865"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HUR16989.1"
                     /translation="MPTTAADASRPAGTLRRLAEEFPRQEFTTLVKRLPRYAKLAWAL
                     ARDPSLSRVRRAAVMAAAAYVVSPIDLVPGFIPVAGQLDDVAVALAAIRLA"
     gene            complement(266..742)
                     /locus_tag="VMZ33_06870"
     CDS             complement(266..742)
                     /locus_tag="VMZ33_06870"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR16990.1"
                     /translation="MQKIITSLWFDTQAEEAATFYSSLVPDSRVVNVTRYGPGAPMPE
                     GTAITVEFELAGQAFNALNGGPAFHFTEATSLIINCDDQAEVDRLWSALAADGGEAGQ
                     CGWLKDRFGFSWQIVPRALGDYLFGSDADAAGRAMQAMLQMQKLDIDEIQRAYDGG"
     gene            877..1866
                     /locus_tag="VMZ33_06875"
     CDS             877..1866
                     /locus_tag="VMZ33_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR16991.1"
                     /translation="MDVITAEGLVKVYRTRKQQVRALDGLDLAVGEGTVLGLLGPNGA
                     GKTTAVRVLATLLRPDTGKATVAGFDVVSQSQQVRAQIGLSGQYAAVDENLTGRENLW
                     MFGRLYQLSSREARARAGELLEQFDLADAGDRVVKTYSGGMRRRLDLASALIGRPRVL
                     FMDEPTTGLDPRSRLGMWDVIRGLVKEGTTLLLTTQYLEEADELANTIAVVDRGKIIA
                     SGTADELKRQVGGERIEVVVRDAAALPRVTDMMKALDTTMSKVTIDEHTRRLSIATNA
                     GANGLVGLVREIAAENIEIDDIGLHRPTLDDVFLTLTGHAAEDKPTPDERTKT"
     gene            1863..2702
                     /locus_tag="VMZ33_06880"
     CDS             1863..2702
                     /locus_tag="VMZ33_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR16992.1"
                     /translation="MTATGRDSDVRRGTGGVRQALRDGGILAWRYLKRVPRIPELAIF
                     AIIQSIMFVLLFAFVFGGAIPLPGGGSYREFLMPGIFAQTIAFASATTAIGMADDMSK
                     GIIDRFRSLPMARPSVLIGRTISDVIYNGGILIVLMAAGFTIGWTWHTGIGELLAGFA
                     LLLLFTYSMSWVGVWLGLSVPNVEVGQQLGFTAIFPLTFISSVFVPPSTLPDFLRPVA
                     EWNPVSTLASSLRGLWGNPNPFVNATGLPNEQPVLVTLMWVGIILAIFIPLAVRKYRS
                     VSR"
     gene            2802..3002
                     /locus_tag="VMZ33_06885"
     CDS             2802..3002
                     /locus_tag="VMZ33_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16993.1"
                     /translation="MVEERANLDYGAEDNSRDDDTSDDTSEGTSDGTSDGTSDGTSDA
                     GVMPATDAGLQPNEEAKTYGAP"
     gene            complement(3126..>4287)
                     /locus_tag="VMZ33_06890"
     CDS             complement(3126..>4287)
                     /locus_tag="VMZ33_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUR16994.1"
                     /translation="VLFTTAVVTAYDYLLRQPDLWGVDVINNSWGNMYRQFDPNDPVS
                     VITKAVFDHGVTVVFAAGNSGTDEMSLNPFSEAPWVISVGAGSVDYKLASFTSTGLIH
                     DNSTAGQIGPGGHTVYTSDRIGVYHPDVVAPGVAISSSCDTAGTVIGPCAPGSNESAS
                     GTSMAAPHVAGAAAILKQANPRLTPTQVRLALQATAKPMVDAAGKVLPFWQQGYGYVD
                     LAKAAALVRSSGWQDKLAVAAGKADSRVLASDGYKVTRSDWWTYPAPRLTVAGTDTQT
                     YTINVPSTVGYLKVTVSHPGLGAVCCNGMYYDVTVKDAAGAVVATGTEADSTGTTSAF
                     VDLRGVSPAVKYGAFTVEVSGFLAASDPDTLDSDSLLGRMITVQVAQVVAGR"
BASE COUNT          742 a         1366 c         1446 g          733 t
ORIGIN      
        1 ggccaggcgg atcgcggcca gtgcaacggc aacgtcgtcg agctgacccg ctacggggat
       61 gaaacccggg acgaggtcga tcggcgacac cacgtatgcg gcggcagcca tgaccgcggc
      121 gcggcgtact cgcgacagcg acggatcgcg cgccagtgcc caggccagct tggcgtagcg
      181 cggcaggcgc ttgacgaggg tcgtgaactc ctgccgtgga aactcctcgg cgaggcggcg
      241 cagggtcccg gcgggccggg acgcgtcagc cgccgtcgta ggcacgttgg atctcgtcga
      301 tgtcgagctt ctgcatctgg agcatcgcct gcatcgcgcg cccggcggcg tcagcatccg
      361 aaccgaacag gtagtcgcca agggccctgg gcacgatctg ccacgagaag ccgaacctat
      421 ccttgagcca gccgcactgg ccggcctctc cgccatcagc cgccagcgcc gaccacagcc
      481 gatcgacctc cgcctgatcg tcgcagttga tgatcagcga ggtcgcttcg gtgaagtgga
      541 aagccgggcc gccgttcagc gcgttgaagg cctggccggc aagctcgaac tcgaccgtga
      601 tcgcggttcc ctcgggcatc ggggcgcccg ggccgtagcg cgtcacgttg acgacccgcg
      661 agtcggggac aagggacgag tagaaggtgg cggcctcctc ggcctgggtg tcgaaccaga
      721 gtgaagtgat gatcttctgc atccaacaag gatgggtcgt cggccaaatg ttcgccgatc
      781 gtcgcgccgg agccgacgcc cggtgcagat ttatgtcaag gttcgaagca agcggcggat
      841 cgccgtgtgg gggcgctgca gaggtatcgc tcggacgtgg acgtcatcac ggccgagggt
      901 ttggtcaagg tctatcgaac gcgcaagcag caagttcgcg cgctcgatgg gctcgatctg
      961 gccgtcggcg aaggaactgt gcttggtttg ctcggcccga acggggcggg taagaccacg
     1021 gctgtgcgcg tcttggcaac gctgctccga ccggacacgg gaaaggccac agtcgccggc
     1081 ttcgacgtcg tgagccagag ccagcaggtt cgcgcgcaga tcggattgtc gggccagtac
     1141 gcggccgtcg atgagaacct gaccggccgc gaaaacctct ggatgttcgg ccgtctgtat
     1201 cagctgtctt cccgtgaggc ccgggctcga gctggtgagc tcctcgagca attcgacctg
     1261 gccgacgccg gcgatcgtgt cgtgaagacc tactcgggcg gcatgcgccg acgactggac
     1321 ctggccagtg ccctgatcgg aaggccccgc gtgctgttca tggatgaacc tacgaccggt
     1381 ctcgatccgc gcagccgtct gggcatgtgg gacgtcatca ggggtctcgt caaggagggc
     1441 acgaccctgc tactgacgac gcagtacctc gaggaggccg acgagctggc caatacgatc
     1501 gccgtcgtcg accgcggcaa gatcatcgcc agcggcacgg ccgacgaact caagcgccag
     1561 gtaggcggcg agcgcatcga ggtcgtcgtc cgcgacgccg ctgcgttgcc gcgggtcacg
     1621 gacatgatga aggcgctgga caccaccatg tccaaggtga cgatcgatga gcacacccgc
     1681 cgactgtcca ttgcgaccaa tgccggagcc aacggattgg ttggtctcgt gcgcgagatt
     1741 gccgccgaaa acatcgagat cgacgacatc gggctgcatc ggcccacgct cgacgacgtg
     1801 ttcctcactc tgactggcca cgcggctgag gacaagccga cgcccgacga gaggacaaag
     1861 acttgaccgc gacaggccgg gattcagacg tccgccgcgg gaccggcggc gtccggcagg
     1921 cactccgaga cggtgggatt ctggcctggc gttacctgaa gcgagtgccg cgcatccccg
     1981 agctggcgat cttcgcaatc atccagtcga tcatgttcgt gctgctcttc gcgttcgtct
     2041 tcggtggcgc gattccgctg ccgggcggcg gcagctaccg cgagttcctg atgcccggca
     2101 tctttgccca gaccatcgcc tttgcgtcag ccacgaccgc gatcggaatg gcggatgaca
     2161 tgagcaaagg catcatcgac cgtttccgat cactgcccat ggcgcgtccc tccgtcctca
     2221 tcggtcgcac catctcagac gtgatttaca acggtggcat tctcatcgtg ctgatggccg
     2281 ccggcttcac gatcggctgg acgtggcaca ccggcatcgg cgagctgctc gctgggttcg
     2341 ccttgcttct cttgttcacc tactccatgt cgtgggtagg ggtctggctg ggcctgtcgg
     2401 ttcccaacgt cgaagtcggc cagcagctcg ggttcactgc catctttccg ttgacattca
     2461 tttccagcgt tttcgttccc ccctcgacgc tgcctgattt cctgcgtccg gttgccgagt
     2521 ggaacccggt cagcaccttg gcctcatcct tgcgcggcct gtggggcaac ccaaacccat
     2581 tcgtcaatgc gacgggcctg cccaatgagc agcccgtcct ggtgacgctc atgtgggtgg
     2641 ggatcatcct ggcgatcttc atacccctcg ccgtgaggaa gtaccgctcc gtcagccgct
     2701 aggcagtcgc aaggtctggg ttacccgatc gcgggtagcc ggactgtcca actggccggt
     2761 ggggcgtcca cgatggaagc tcacttagca ggaggacagc aatggtcgaa gagcgcgcca
     2821 acctcgacta cggggccgag gacaacagcc gggacgacga cacgagcgac gacaccagcg
     2881 aaggcacatc tgacggtacg tcggacggga ccagcgatgg cacctccgac gccggcgtca
     2941 tgcccgcgac ggatgcgggc ctccagccga acgaggaagc gaaaacgtac ggggcgccgt
     3001 agcccagagg ctgagcctcg cataagaaaa gacggctcgg ccgaagccga gccgtcctgc
     3061 cgtgcgcggg ttggtcacgg ggcctcacag cggcccccga ccatttccgc gaagggtgat
     3121 gcgagttatc tgccggcgac gacctgcgcc acctggacgg tgatcatccg gccgagcagc
     3181 gagtcgctgt cgagcgtgtc cgggtctgag gctgcgagga acccgctgac ctcaaccgtg
     3241 aaggcgccat atttcactgc gggcgagacg ccgcgcaggt caacgaaggc gcttgtcgtg
     3301 cccgtggagt ccgcttcggt cccggtggcg acgacggccc cagccgcgtc cttgaccgtc
     3361 acgtcgtagt acatcccgtt gcagcacacg gcgccgaggc ccgggtgcga cacggtgacc
     3421 ttcagatagc cgaccgtgct cggaacattg atcgtgtatg tctgagtatc ggtgccggcg
     3481 acggtcagcc gcggcgccgg gtaagtccac cagtcagagc gcgtcacctt gtatccgtcc
     3541 gaggcgagga cacgtgagtc ggctttgccg gcggcaacgg ctagcttgtc ctgccagccg
     3601 ctgctgcgga cgagtgccgc tgccttggcc agatcgacgt atccgtaacc ctgctgccag
     3661 aacggcagta ccttgccggc ggcgtcgacc atcggcttcg ccgtcgcctg gagggcgagg
     3721 cggacctggg tcggcgtaag ccgcggattg gcttgcttga ggatcgcggc ggcgccggcg
     3781 acgtgtggcg cggccatgct cgtgcctgac gcgctctcgt ttgagcccgg cgcacacggg
     3841 ccgatgaccg tacccgcggt atcgcaggag gatgagatcg ccacgcccgg cgcgacgaca
     3901 tcagggtggt aaacgccgat ccggtcactg gtgtagacgg tgtggccccc ggggccgatc
     3961 tggccagcgg tgctgttgtc atggatgagg ccggttgagg tgaagctggc gagcttgtag
     4021 tcgaccgagc cggcgccgac ggagatgacc cacggcgctt cgctgaacgg gttgaggctc
     4081 atctcgtcag tgcccgagtt gcccgccgcg aagaccaccg taacgccgtg atcgaacacc
     4141 gccttggtga tgaccgagac cggatcgttg gggtcgaact ggcggtacat gttgccccag
     4201 gagttgttga tcacgtcaac gccccacaga tcgggctggc ggagcagata gtcgtatgcc
     4261 gtgacgacag cggtcgtgaa caggacg
//
LOCUS       DATLCO010000300         6182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4325118, whole genome shotgun sequence.
ACCESSION   DATLCO010000300 DATLCO010000000
VERSION     DATLCO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4325118"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1170
                     /locus_tag="VMZ33_06895"
     CDS             262..1170
                     /locus_tag="VMZ33_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUR16995.1"
                     /translation="MTNDGRADVVPVSPTAWNDLDLRVAETVCADLGAIRLLPSVRAT
                     AAQISESQLDASIARLRRLDLVSQGAPLRLVVRLQNEKGFSRSALRDAVARRLGSRVT
                     RGHEEANEVWLIQDTRRSVRVGLRVKALEPVRPTRAAERPGSLRPAIAAGMIGLGGQR
                     PGRLLDPCCGSGTILGEAQLAGWHTVGGDYAIDALESTSKNFASDVVRLDARRLPFRD
                     NEFELVVSNLPFGHQYEMQGAPVAWYRRTLNEALRVAPRAILLAPPSPPFRQALGRLK
                     VALAERHPVEVLGRPATIWVIERVTR"
     gene            1754..1900
                     /locus_tag="VMZ33_06900"
     CDS             1754..1900
                     /locus_tag="VMZ33_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR16996.1"
                     /translation="MAVDLYDGCVLYDFSTRTVKLIDLDHYRRSPYLLDVESSARLVE
                     LYGS"
     gene            complement(2240..3220)
                     /locus_tag="VMZ33_06905"
     CDS             complement(2240..3220)
                     /locus_tag="VMZ33_06905"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HUR16997.1"
                     /translation="MSDAEDIGTWQAAWNTPRDVVDAVVRRAAGSGVVVDERILEGHG
                     NEVYGVRTADGQDLIVRICRRQGAGLDCERWPISAALAAGVPAPPILLIERLDLNGEE
                     LSIQVQQRLPGRTVHRLFGELSDAQLGRLTTGAGEILAMIHAIQPSGPGPIDPAGSCA
                     ALPSTAGDAAVAAMIRRNGELTTSGINTQLLQTAVEAVETGAALLDAAPVCLIHGDWR
                     TTNVLSDGDSITGVVDWEGARGGDAAFDFAGVWTVASRRKATSTELLLDAYRAAGARL
                     DDAFHARRLLYSIADLHSALGHFVSTDRPDLLKQAVDDLHANLRQLDDLT"
     gene            complement(3296..3808)
                     /locus_tag="VMZ33_06910"
     CDS             complement(3296..3808)
                     /locus_tag="VMZ33_06910"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HUR16998.1"
                     /translation="MTTDEIHMARALEIAAAGMRAGEMPIGAVVAVDGDVIAEAHTQE
                     RTQNRLLVHADLLALDAADSKLAGRRRRATLYVTLEPCIMCVGAAFTMRVGALVYALE
                     SPSDGGCQTLLDWDKARNAESMPGYTVPAIRSGLMRLESALLFRQYAGTAQAGWTKTW
                     AEELAALGGA"
     gene            complement(3904..4878)
                     /locus_tag="VMZ33_06915"
     CDS             complement(3904..4878)
                     /locus_tag="VMZ33_06915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR16999.1"
                     /translation="METIGPGEFGRITGLSAKAVRIYEDRGLLAPSAKDVGSGYRRYT
                     AADINRAGRIALLRRAGISLSQIRRFLADPSLSMIDSWLRDVDSERNERRNALNALAR
                     ALGLGSPTYEEDVMAVTIRSVTSPDELGDVFDAAGAQFDPSIDRSDDKRFGDLRAAFE
                     AGERDLLLVAEIDGAFAGAALGFSGQPSATLRMLATDSQYRRRGLGRALLRAFEAAVE
                     RAGIESVALGADEQAGFYVRHGYQTMLLLQWVYAGDAHAREREELLAGPLRDSTTFDS
                     TFNGVPQLFAILGEPDPSIRSRVSDIAIGAHVGYCMTKKVRRLEPSPV"
     gene            complement(5255..>6182)
                     /locus_tag="VMZ33_06920"
     CDS             complement(5255..>6182)
                     /locus_tag="VMZ33_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17000.1"
                     /translation="FFGSDGHIRRDADVVATLVEAARSDPRLGEVVATIDDIPAGFGA
                     FGVLLAGQTHSRSDIVVKVNAQPIERAWLTALDLVDPGVVPHVVAQGDVLAALPLGWL
                     AMERLGHQPPGMGAAEWYPPLLRGTDRWQAAAVSIDDLPVVETIDDAWISRWLDVALA
                     DSADAHLRRLRDRFQEDWAWVVDQCPVVPCHGDVHFFNAGSRSGSADEPLVLFDPIPR
                     LAPWAYDAANTQALTNYHRFADGRPSLVEQAAVLRGARGLDVPDGPTMQRVSALLCAW
                     LAVCWSVWFSQVAPELRGTAMDYVKRAVAIDR"
BASE COUNT         1060 a         2065 c         1997 g         1060 t
ORIGIN      
        1 cctgcctcgc cgatggccat ggcaactcca gttcatgacc gccgccgtcc ggctccgcaa
       61 cctgccgctc gtctgctgaa caacagcagt ggacgacgcc cgccatcccc gacggcgtca
      121 ggaactcaac cgaccaagac ccgcctgctt caacgcgacc caacctcgac gaccgagagc
      181 tgcgatcggc gcggaagcct caaccccagg aagccccgca atcagatcca caacggtgga
      241 tcgaggctaa gcccaggtct tgtgaccaac gacgggcgcg cagacgtcgt gccagtgagt
      301 ccaacggcct ggaatgacct cgaccttcga gtggccgaga cggtctgcgc tgacctgggc
      361 gcgatccggc tcctcccctc cgtccgggcc actgccgctc agatctccga gtcccaactg
      421 gacgcatcga tcgcccgtct ccgccggctg gacctggtct cccaaggcgc accactccgg
      481 ctcgtcgtgc gactccagaa cgagaaaggc ttttcgagga gcgctctgcg tgacgccgtc
      541 gcccgtcggc ttggctcgcg ggtcaccaga ggccatgagg aggcgaacga ggtctggctc
      601 atccaagaca ccagacgctc cgtgcgggtc ggcctccgtg tcaaagccct ggagccggtg
      661 cgcccaacga gagccgccga acgaccgggc tcgcttcggc cggccatcgc cgcggggatg
      721 atcggtctcg gtggccagcg acccgggcgg ctcttggatc cttgctgcgg cagcgggacg
      781 atcctcggcg aagctcagct cgcaggctgg cacaccgtag gtggcgacta cgccatcgac
      841 gccctcgagt cgacatcgaa gaatttcgct tccgacgtcg tccggctgga cgcgcgccgg
      901 ctgccgtttc gcgacaacga gttcgaactt gtcgtctcca acctcccctt cgggcatcag
      961 tacgagatgc aaggcgcccc cgtggcgtgg tatcgccgaa cgctcaatga agcgctgcga
     1021 gttgcgccac gagcaatcct tctcgccccg ccgtcaccgc ctttcagaca agcactcggt
     1081 cggttgaagg tcgctttggc cgagcgtcac cccgtcgagg tccttggtcg accagcaact
     1141 atctgggtca tcgagcgagt tacgcgatga cggagcgaac ctcggcatcc gcgcctccgg
     1201 tcgcggcgct gatccggcct ttggggaccc ccctcttgtt gcaattcgta cgtcgaaatt
     1261 ggcgtgactc cggcggggta cgacgaccat gatgcgccgc cgtgcttgct gactggccaa
     1321 caaagacaag tgcgacggtg cacgagtttc tcgtcgagcg cagctgcgcc gtcgtccgac
     1381 acttcggtga acgccgcgac tctcgctccg tggtcctcgg ggtcgagtct ccaaccgggg
     1441 agcggtacgt ggtgaagcac gccgaggatc ggaagcaatc ggctggctcg aatccgctcg
     1501 cgcgttccac gctgacgtcc agcatccggc gatcccatcg gtcgtccact ccttcgagac
     1561 ggccactggc cttggcctcc ttgagcagtg gggcgaaggc gagattctca cggacagcta
     1621 cgacgacgcc atccttcctc cggatcacaa tcagtcgacg tatcgccgct tcctcagcct
     1681 cccggtcgat gagatagtcg acgggatcgg acaactggtc gcaacccaca tcgcggtcgc
     1741 cgaggccggg tacgtggctg tcgacttgta cgacgggtgc gtcctatacg acttctcgac
     1801 gcggacagtg aagctcatcg acctcgatca ctaccgacgg tcgccctacc tgctcgacgt
     1861 tgaatcgtca gctcggctcg tcgagctata tggctcctga ggagtttcga cggggctcga
     1921 cgattgatga gcggacgatg gtgcacaccc ttggccggat ggccttggtg tacctcggct
     1981 gcgatcgcaa gagcgcggct cgcctcgacg acttccgtgg cagcgaggaa cagttcacaa
     2041 cggcgacgaa ggcgtgcagc gcggatcccg atcgccgctt tcagaccgtc gcggagttcg
     2101 gagctgcgtg gcggagcgca agcggagcat gatcgccacc ggcactcagt cccggccatc
     2161 tgggacagtc agccgctccg cagcacggcc gcgcaaacga gtaggcctgc cgctgctggt
     2221 ttggctggtg tccgctgcgt catgtcaggt cgtccagctg acgcaggttc gcgtggaggt
     2281 cgtcgacggc ctgctttagg aggtctggcc tgtctgtgga cacgaagtgg ccgagtgcgg
     2341 agtggaggtc ggcgatgctg tagagaaggc gtcgagcgtg gaacgcgtca tcgagccgag
     2401 cacctgcggc gcggtatgcg tccaggagga gctcggtaga agtcgccttg cggcgcgacg
     2461 ccaccgtcca gacgcctgca aagtcgaacg cggcatctcc gccgcgtgcg ccttcccagt
     2521 cgacgactcc ggtgatcgag tctccgtctg agagcacgtt ggttgtccgc caatcaccgt
     2581 ggatgaggca taccggcgcg gcgtcgagca gcgcggctcc ggtctccacg gcctcgactg
     2641 cagtctggag aagctgagtg ttgatgccac tagtggtcag ttcgccgttg cgtcggatca
     2701 tcgccgcgac cgcggcgtcg cctgcagttg aaggaagggc agcgcaactg ccggcggggt
     2761 cgatcggtcc tggaccggag ggctggatcg cgtggatcat cgcgaggatc tcgcccgccc
     2821 ctgtcgtcag cctccccagt tgcgcatcgg agagttcgcc gaagagccga tgaacagtcc
     2881 ggcccggcag acgttgctgg acctggatgg acagctcctc gccgttcaag tcgaggcgct
     2941 cgatgagcag gatcggcggc gcgggcacgc cggccgccaa ggcagcagag atcggccacc
     3001 gttcgcagtc gagcccggca ccttgcctcc gacaaatacg cacgatcagg tcttgaccgt
     3061 cggcggtgcg aacgccgtag acctcgttcc catggccctc gagaatcctc tcgtcgacca
     3121 cgactcctga tccagcagcc cggcggacca ccgcgtcgac cacgtcacga ggcgtgttcc
     3181 atgccgcctg ccaggttccg atgtcctcgg cgtcgctcac gacatgtcag tctgcgacaa
     3241 cggcagcgcg cggcggtccg atttcctacg tcacttggcc ggccatgtgg ggcagtcagg
     3301 ctccgccgag cgcggcgagc tcttcagccc acgtcttggt ccaaccggcc tgggcggttc
     3361 ctgcgtactg ccggaagagg agcgctgatt cgaggcgcat cagcccggag cgaatcgctg
     3421 ggacggtata gccgggcatg gactcggcgt tgcgtgcctt gtcccagtcc agaagtgttt
     3481 ggcatccacc gtcactgggc gactccaggg cgtagaccag tgctcccact cgcatcgtga
     3541 aggccgctcc gacgcacatg atgcagggct cgagggtcac gtagagcgtt gcgcgtcgtc
     3601 gccgtcctgc gagcttcgaa tctgcagcgt ccagcgcgag gagatcggcg tgcacgagca
     3661 ggcggttctg cgtgcgttcc tgggtatgcg cctcggcgat cacgtcgcca tcgacggcaa
     3721 ccaccgcgcc gatcggcatt tcgccggctc gcatgccggc agctgctatc tccagagccc
     3781 gagccatgtg gatctcgtcg gtggtcacct ggtgattgtg cctgcttcga ccgcgtggct
     3841 cggcgtcccc gtcaacggtc cgtcagaggc ggctcggacg agaagctcgc ctcgcatcga
     3901 cgatcaaacg ggcgacggct caaggcggcg gaccttcttc gtcatgcagt atccgacgtg
     3961 cgcgccgatc gcgatgtcgc tcacccgtga acggatggag gggtctggct ccccgaggat
     4021 cgcaaacagt tgtggcacgc cgttgaatgt cgagtcgaag gtcgtcgagt ccctcaaagg
     4081 accagcgagt agttcctctc gttcacgtgc gtgggcatcc ccggcgtaga cccactgcag
     4141 aaggagcatc gtctgatatc cgtgccggac atagaagcca gcctgttcgt ccgcgccaag
     4201 agcaacgctc tcaatcccgg ctcgttctac tgccgcttca aaggcccgaa gaagcgcgcg
     4261 accaagtccc cgtcgacggt actggctatc ggtcgcgagc atgcgcaacg tggcgctggg
     4321 ttgcccgctg aaccccaacg cggcgcccgc gaaggcacca tcaatttcag cgaccagcag
     4381 gaggtcgcgc tcgccggcct cgaaggcggc gcgcaggtca ccaaagcgct tgtcgtctga
     4441 ccgatcgatc gacggatcga actgcgctcc cgctgcgtcg aacacatcac ccagttcgtc
     4501 aggcgacgtg acgcttcgaa tggtgactgc catgacgtcc tcctcgtagg tcggtgagcc
     4561 gagaccgaga gcccgggcga gagcgttcag cgcgtttcgg cgctcgttgc gctccgaatc
     4621 gacgtcccgg agccacgagt cgatcatcga gagacttgga tccgccagga atcggcggat
     4681 ctggctcaac gagatcccag cccgccgcaa cagcgcgatt cgtcccgccc ggttgatgtc
     4741 cgcggcggtg taccgccggt agccagaccc gacatccttt gccgagggcg ccagcagccc
     4801 gcggtcctcg tagatccgaa ccgcctttgc cgacaggccg gtgatacgtc cgaactcgcc
     4861 tggtccgatc gtctccatgc cgtcagcctg caacccgccc caagggcgaa gtcaacgcat
     4921 tctttgaggc cgtcgacctc gtccccgaga tgcggggcga tgcggccaga atccgccagc
     4981 ctcgcacggc gcagtctgac tggctccctc gcgggacaag cttctcgagg agcgaccgcg
     5041 tctcggcctt cggggacacc gatgcggcgc tctcgggact cgcgtctggg acaggtcccg
     5101 tcgggccatg gtgacagacc gccgactccg ggtcgccctc gaagtcgacc tcaagcgacg
     5161 cgggttagcg gggaggttgc cgcgctccgc tcgcagaggt ggtccggctc gaacgatgcg
     5221 cgagctccgc cgcgtgtcgc tcggtgccgt ccattcaacg atcgatggcg accgctcgtt
     5281 tgacgtagtc catcgccgtg ccgcgcagct cgggtgcgac ttgcgagaac caaactgacc
     5341 agcaaacggc gagccacgcg cagagaagag ctgatacccg ctgcatggtc ggcccgtcgg
     5401 ggacgtccag ccctcgtgct ccacgaagga ccgcagcctg ctctaccaga ctcggccggc
     5461 cgtcggcgaa gcggtggtag ttcgtgagtg cctgagtatt ggcggcgtca taggcccatg
     5521 gcgcgagccg aggaatcggg tcgaagagca ccaagggctc gtcggcggac ccactgcgac
     5581 tgccggcgtt gaagaagtgg acatcgccgt gacagggcac caccggacat tggtcgacga
     5641 cccaggccca gtcctcctgg aaccgatccc gcagccgacg aaggtgggca tcggcggagt
     5701 ctgcgagcgc gacgtcgagc cacctcgaga tccacgcgtc gtcgatcgtc tcgacgaccg
     5761 ggagatcatc gatcgataca gcggccgctt gccaccggtc ggtgccacgc aggaggggtg
     5821 ggtaccactc ggctgccccc atacctggtg gttggtggcc gaggcgttcc atggcaagcc
     5881 atccgagcgg aagcgcggct aagacgtcgc cctgggcgac cacgtgaggc acaacgcctg
     5941 ggtcgaccaa gtccagagct gtgagccagg cccgttcaat tggctgagcg ttgactttga
     6001 cgacgatgtc ggagcggctg tgagtctgtc ctgccagcaa tacgccgaag gctccaaagc
     6061 cagcggggat gtcgtcgatc gtggcgacga cttcgcccag tcgtggatcg gaccgtgctg
     6121 cctcgacgag cgtcgccacg acatccgcat cgcggcggat gtgtccgtcg ctgccgaaga
     6181 ag
//
LOCUS       DATLCO010000301         8393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9564791, whole genome shotgun sequence.
ACCESSION   DATLCO010000301 DATLCO010000000
VERSION     DATLCO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9564791"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1381)
                     /locus_tag="VMZ33_06925"
     CDS             complement(47..1381)
                     /locus_tag="VMZ33_06925"
                     /inference="COORDINATES: protein motif:HMM:NF020859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HUR17001.1"
                     /translation="MTLNGGEGSFEDNVRAALPTDADLKQVAIESLIQRERERGFLTG
                     ADYAAAGKFVNRSQRQVQRWMREDRVASGTKTRDRHRERFELTDLMISAVYANAGNLR
                     AAYAQLCEAQILTCCSETSFRNAWARLPVAIQRYPTIGREAIRTYGLYPAWTAPVPND
                     VWQLDSHDIDLFCRFGGDVIRPVVTDIIDDCSRLIVGSILTPDAPRASDIAAVAAMAL
                     RTREAPDGTVIGGAPRLVLYDCALAHSAELIARGWSQLGIVPRSTQPYEPTQKGKKER
                     HYRTLEAECFRELPGYITPNALTKEGEYLYRPHTSELLTYEQVLKRLLEYTNYYNFER
                     PHQGIGGRRPIDVVRAHAVTPRTIDRAVLARAFWRTKRETYTVQKRGVHVERYYSSWA
                     LIEHVGKKVIVRYDPTDPDFIAVNRTGFRGVSVSMTEDVTHGNSSEVPTGAS"
     gene            complement(1371..1733)
                     /locus_tag="VMZ33_06930"
     CDS             complement(1371..1733)
                     /locus_tag="VMZ33_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17002.1"
                     /translation="MDALAGVLTWLRYQWPPTSWRLVAVEAEISPGGRPDLWWQVGGM
                     FVADEVKTGLPARSWETQFAQQRANGNSQYGNRFLGLRVFATSDPCRSLWLGRDGVAR
                     RLHTTPFWFPSMEHRDDA"
     gene            2073..2693
                     /locus_tag="VMZ33_06935"
     CDS             2073..2693
                     /locus_tag="VMZ33_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17003.1"
                     /translation="MTNFSGGRFDTGFRESWLAIGAANWLFWFIPGWHDVRVEVWFEQ
                     DAGQHVEVHGLTVSAYLEGLTPDGKSAPQSLTATDLRQIPLGGLTSFLGAAGPARALE
                     RSAEAADDAEPVFDPSRLTAAGAFDAAMSMPLVQLDRTVRIKNPGGSKPDEFYRTVAD
                     AFGRLALVSASPASDIASANNVPVTTVHRWVKEARRRRFLPPARPS"
     gene            complement(2919..3134)
                     /locus_tag="VMZ33_06940"
     CDS             complement(2919..3134)
                     /locus_tag="VMZ33_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17004.1"
                     /translation="MRAATVQQWKFRKLLPPAECTVNALDAWSVDAIWRWAEKTGRLP
                     VFGPTGEAAALVERATPEAASEGAQSA"
     gene            complement(3608..3799)
                     /locus_tag="VMZ33_06945"
     CDS             complement(3608..3799)
                     /locus_tag="VMZ33_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17005.1"
                     /translation="MTSNNDVQGRVEDVFVVYRPRAGGSEVTGVYATVEGVHEAIDEC
                     PESWFESFELQGCHSEGDT"
     gene            3953..5134
                     /locus_tag="VMZ33_06950"
     CDS             3953..5134
                     /locus_tag="VMZ33_06950"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HUR17006.1"
                     /translation="MATELAPVTPSVMKWARETIGVSLEEAARRAGVKPERVQEWESG
                     KKEPTVAKLRTLAKFYQRPLSVFFLPEPPPDFSTIRDFRRNPGQDDLLWSLSLHKVYR
                     RALEQQEITEDILDDDGEHALVRVPSAAISDGAETVADVARQALGVSLREQFSWRRPE
                     EAFAGWLQAVEDLGVFVLRTSDVSVDEMRGLSISSGAVPVIVINALDWPRGQVFTLAH
                     ELVHLILRQGGLCDLLEPSAGSARRVETFCNAAASAMLMPEDAFLDSSVVGPPGEREW
                     EDEVLVQLGARFGVSQEAVLRRLVTLKRASMDFYLQKRQEYLEIYHEQREEEKERRRQ
                     RDSQGGPPPHRMAIRDRGKPYVRLVLDAYHRDVIGPSTLSSFLGLKLRHIPALEHEVR
                     G"
     gene            5134..5592
                     /locus_tag="VMZ33_06955"
     CDS             5134..5592
                     /locus_tag="VMZ33_06955"
                     /inference="COORDINATES: protein motif:HMM:NF025725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HUR17007.1"
                     /translation="MYVFDTSALVEAWVRLYPPRRFPALWENIDELINDERAVAPHEV
                     LEELKAQDDDLLQWAKDRSDGFIRPTSRQVMLAARDVLEDHPKLTKPGKGRGKADPFV
                     VALAETIDWTVVTQERGGTAEKPKIPYVCGERGVNCMSDLDIIDAEDWTF"
     gene            complement(6431..6739)
                     /locus_tag="VMZ33_06960"
     CDS             complement(6431..6739)
                     /locus_tag="VMZ33_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17008.1"
                     /translation="MPNEATHAVLSTFNVDPARRDDQEFGLRNVIVPGVREAPGFVSG
                     IWTRTTDGTKSTVLVAFSAEADAREFQASVQANASNQAAVGLTLVDVELVEVNAIAGA
                     "
     gene            6843..7748
                     /locus_tag="VMZ33_06965"
     CDS             6843..7748
                     /locus_tag="VMZ33_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HUR17009.1"
                     /translation="MAATSMLLYDPQHADSEIVTAAGFLAGYSGRTREAYSLDLRQFI
                     AWCHSHELSLFEVQRTHIELFARELEMHGRARATISRRLATIVGFYRFAEEEGVIAHS
                     PAVHVRRPRLTYESNAIGLDRNELGALLVAAGLAGPRDHALCSLLALNGLRVSEAIGA
                     LIEDLAIERGHRTLLVHRKAGKTVRIPLAPRTARAVDLIVGERFEGPIFANSEGRALD
                     RHSAARIVRRVAKRAEIPKKIGPHSLRHAFITAALDAGVALRDVQDAASHADPRTTMR
                     YDRGRQSLDRHPTYVVAAFVAGAAR"
     gene            7799..8254
                     /locus_tag="VMZ33_06970"
     CDS             7799..8254
                     /locus_tag="VMZ33_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17010.1"
                     /translation="MKVRRFVTVVGVFLVLAAAAFSFHLAMERAKVDLAEAPLCEVGA
                     EFSGMSCRAVRSGVILEASRREFVVMVDGRRRLVMDVTLKGTIHAKPGDVVSTQLWEG
                     QLMRVVGTGFDVRSNSAPENRSFGFRVMAIFFLVMALPGGLAAGFDRRS"
BASE COUNT         1432 a         2740 c         2704 g         1517 t
ORIGIN      
        1 ccggccgcga acccgtcgcc actcccggac catcccgacc gcgttctcac gaagctccgg
       61 tgggtacttc cgaactgttc ccatgcgtaa catcctccgt catagagacg gagactccac
      121 ggaacccggt gcggttcaca gcgatgaagt cgggatcggt ggggtcgtac cgaacgatca
      181 ccttcttgcc tacatgctcg atgagagccc agctcgagta gtacctctca acgtgcacgc
      241 ctcgcttctg gactgtgtag gtctcgcgct ttgttcgcca gaacgcgcgg gccagcacgg
      301 cgcgatcaat cgtccgtggt gtcacggcgt gggcacgtac gacgtcgata ggtctccggc
      361 cgccgatacc ttgatgggga cgctcgaagt tgtagtagtt ggtgtactca aggagccgtt
      421 tcagcacctg ttcgtacgtg agcaactcgc tggtgtgagg ccggtagagg tactcgcctt
      481 ccttcgtcag cgcgttcggt gtgatgtagc caggtagttc acgaaagcat tcggcctcga
      541 gggtgcgata gtgccgctcc ttcttgccct tctgtgttgg ctcgtacggc tgagtcgagc
      601 gcggcacgat cccgagttgt gaccaaccgc gtgcgatcaa ctcggctgag tgcgccagag
      661 cgcagtcgta gagaacgaga cgtggggctc caccgatgac cgtgccgtcg ggcgcttcgc
      721 gcgtgcgaag ggccattgcg gcgacggccg cgatgtccga cgcccgcggg gcgtcggggg
      781 tgaggatcga tccgacgatg agccgcgagc agtcatcgat gatgtcggtc acgacaggcc
      841 ggatcacgtc gccgccaaac ctgcagaaca agtcgatgtc gtgtgagtcc agctgccata
      901 cgtcattcgg aacaggcgcc gtccaggccg gatacagacc gtaggttcgt atcgcctcac
      961 gcccgatggt tgggtaccgt tggattgcga ccggcaaccg cgcccatgcg ttgcggaacg
     1021 aggtctcaga gcagcacgtg aggatctggg cctcgcagag ttgggcgtac gccgcacgga
     1081 ggttgcccgc gttggcgtac accgcagaaa tcattaggtc cgtaagctcg aaccgttcgc
     1141 ggtgtcgatc gcgggtcttg gtgcctgatg cgacgcggtc ctctcgcatc caacgctgta
     1201 cctggcgctg cgatcggttg acgaacttcc cagctgcggc gtagtcagcg ccggtcagga
     1261 acccacgctc ccgttcgcgc tggatgaggc tctcgatggc gacctgtttc aggtccgcgt
     1321 cggttggcag cgccgcgcgg acgttgtcct cgaacgaccc ctccccgccg ttaagcgtca
     1381 tcgcgatgct ccatcgaagg gaaccagaag ggcgtcgtgt gcaagcggcg cgcgacgcca
     1441 tcacgtccga gccaaaggga ccggcacgga tcggacgtcg cgaagactcg gaggcctaag
     1501 aatcgattgc cgtattggct gttgccgttc gcacgctgct gcgcgaattg cgtctcccac
     1561 gagcgcgcag gaagtccggt tttgacttcg tctgcgacga acatgccgcc gacttgccac
     1621 cagaggtcgg gccgtccacc tggcgagatc tctgcctcca cggccacgag gcgccaagaa
     1681 gtcggcggcc attggtaccg gagccacgtc agaacgccag ccagcgcgtc caccttcgcc
     1741 gctcgcatga cgggcgtagg cagcccatga aagtgcgggc tgaccgccgt gagtgcatcg
     1801 gaacgcgagg gacgccgtgt ccgcgggccc tgggccagct cagtaattgc ggcgtgctgt
     1861 gcgatgccga tccaggcccg ccggtgacgc gccccgagga gcaacggatg aagggatcgc
     1921 gcatcgcttt ggaccggcga gaccggcgtc cgtcgggaag gctgtctatt gctgcagcgg
     1981 tggactgtca ttcccgcagt gtgactgagt gggctcggga atgcaagtgg tgcagccata
     2041 gacgtccgca gctgcctgtg tagagtcccg gtatgacgaa tttcagcggc ggccgtttcg
     2101 acacgggctt tcgagagagc tggctggcaa tcggcgcggc caactggctc ttctggttca
     2161 ttcctgggtg gcacgacgtc agggttgagg tgtggttcga gcaggacgct ggtcaacacg
     2221 tggaggtcca tgggctcacc gtgtcggcgt accttgaagg tctcacgccc gacgggaaga
     2281 gtgcgccgca gtcgctcact gcgacggacc tgcggcaaat cccccttggt gggctcacaa
     2341 gtttcttggg agctgccggc cccgccaggg cgctcgaacg ctccgctgaa gcggccgacg
     2401 acgccgagcc tgtgtttgat cccagtcgcc ttacggccgc gggggcgttc gatgccgcga
     2461 tgagcatgcc gctcgttcag ctcgaccgga ctgtgaggat caagaatccg ggcgggtcga
     2521 agccggacga gttctaccga accgtcgccg acgccttcgg gaggcttgcg ctcgtcagcg
     2581 caagtcccgc gagcgatatt gcctctgcca acaacgtgcc agtgacgact gtccaccgct
     2641 gggtcaagga ggcccggcgt cgacgatttc tgccgcccgc ccgaccctcc tgaaccgcat
     2701 catccccacc gcttcggggc gacgctcgta gcgtcacggc gtggcgcgca aggtcgaccc
     2761 ggaagagatc gtcggtgctg ccgaaatcgc tgagcggctc ggcctcgcca ttccgcagac
     2821 ggtgcacgat tggcgccgac ggtacgagga ctttccgaag ccactcgttc aactccgcat
     2881 gggcgtaatc tggcattggc ctgagatcgc cgcgtgggct aagcggactg ggcaccctcg
     2941 gaggcagcct ccggcgtagc ccgctcgacc agggcggcgg cttcaccagt gggaccgaag
     3001 acggggagtc gtccggtctt ctcggcccac cgccagatcg catcgacgct ccaagcgtca
     3061 agagcgttga ccgtgcactc agctggcgga agcagctttc ggaacttcca ctgttgcacc
     3121 gtggcggccc gcacgcgaag cagctgggcg acttccctag cgccgatggg aggagcgtcc
     3181 tgccgcggtt tccaatcaga gggcgtaccg gagggcagcc gactagctct tgtgtcggct
     3241 gccctcacgg ccgcctccac gcggcctggc cgaccatttg gttctccgct cgtcgtgcat
     3301 gtgatcatat gcaaacagta tcaccacaac gatcggttcg ctacctgggc tcgtcgggta
     3361 ctcgcaccct cccagtcgcg attgcccact cgacgacggc ccgtctcgtc cacgccgggg
     3421 agcacttgac ggtcgcgcca tctgcgggta gttcgccccg gcgctcccac agttcaacgg
     3481 cctcgagtgg cactcccaag aaggtcgcga tttcatcgat gcccatgggc ctgccgcccg
     3541 cttcgctgga atccatcctc tgagtgttgc atacgattgc atgcaaccca ataggccctg
     3601 gatagcccta cgtgtcccct tcgctgtggc agccctgcaa ctcaaagctc tcgaaccagg
     3661 actctgggca ctcatcgatg gcctcgtgaa cgccctcgac tgtggcgtag acgccggtga
     3721 cctcggagcc accagcccgt ggccggtaca cgacgaatac atcctctacg cggccttgca
     3781 catcgttgtt cgaagtcatg tgcctacggt cccacaacgg tgagacagcg tcaccggaga
     3841 tgcggtccgc cgtggctgac aactacagaa ggtccgccgc ctcttccctg ctcgggtacc
     3901 ccgcagagga ttcgtgtccg atggcgtaac tacgctcgca gggaggttcc ccatggccac
     3961 tgaactcgcc cccgtcacac cgagcgtcat gaagtgggca cgcgagacca tcggcgtgtc
     4021 gctcgaggag gctgcccgac gggccggcgt gaagccggag cgggtccaag agtgggagtc
     4081 aggcaaaaag gagccgacgg tcgccaagct gcggacgctg gcgaagttct accaacgtcc
     4141 gttgtcggtg ttcttcctgc cagagccccc acccgacttc agcaccatcc gggacttccg
     4201 ccgcaacccc ggtcaagacg atctgctgtg gtcgctgtcg cttcacaagg tgtaccgccg
     4261 agctctcgag caacaggaga tcaccgagga catcctcgat gacgacgggg agcacgcgct
     4321 cgtccgagtc ccttccgccg cgattagcga cggcgccgag acggttgcgg acgtcgcccg
     4381 ccaggcgctt ggcgtctcgc tacgcgagca attctcgtgg cgacggccgg aagaggcgtt
     4441 cgccggatgg ctccaggccg tcgaagatct gggcgtcttc gtacttcgta caagcgacgt
     4501 ctcggtcgac gagatgcgag gtctctccat ctcctccggc gcagtgcccg tcatcgtcat
     4561 caacgcactc gactggcctc ggggtcaggt gttcaccctt gctcacgagc tagtgcatct
     4621 gatcctccgc caaggcgggc tctgcgacct tctcgagcca tctgcgggtt ccgcaaggcg
     4681 cgttgagacg ttctgcaacg ctgccgcttc tgcgatgctg atgccggagg acgcctttct
     4741 cgacagcagc gtcgtaggtc caccaggtga acgcgagtgg gaggacgagg tcttggtcca
     4801 gctcggcgcc cgctttggcg tgagccagga ggccgtactt cggcggctcg tcactctgaa
     4861 gcgtgcgtcg atggacttct atctgcagaa gcggcaggag tacctcgaga tctaccacga
     4921 acagagggag gaggagaaag agcgccgccg gcagcgggac tcgcagggcg gaccgccccc
     4981 gcatcggatg gcgatccgcg accgtggcaa gccctacgtc cggctagttc tcgatgccta
     5041 ccaccgtgac gtgatcgggc cctcgacgct ctcgtcgttt ctcgggctga agctccgaca
     5101 cattcccgcc ctcgaacacg aagtgcgcgg ctgatgtacg tgttcgacac gagcgccctc
     5161 gtcgaggcgt gggtacggct gtacccaccg cgccggtttc ctgcgttgtg ggagaacatc
     5221 gacgagctga tcaatgacga acgcgccgtg gcgccgcacg aggtgcttga ggagctcaag
     5281 gcgcaggacg acgacctcct ccagtgggcg aaggaccgca gcgacggctt catcaggccg
     5341 acctctaggc aggtgatgct cgccgctcgc gacgtcctgg aggatcatcc gaagttgacc
     5401 aaaccgggga aggggcgcgg gaaagcagac ccattcgtcg tcgctctcgc cgagacgatc
     5461 gactggacgg tcgtgaccca ggaacggggc gggacggcgg agaagccgaa gatcccctac
     5521 gtctgcggcg aacggggcgt caattgcatg tcggacctcg acatcatcga cgccgaggac
     5581 tggacgttct gacgccgctt tcggcagccg tcactgacaa ctgccgcttc ccatcgccgc
     5641 caacaggttc gtcgatgagt cccatcctcc tgagccaccc acgcatacgc cagtgcccca
     5701 gcagcgccga acggcacttc ggcgctgtca ggacaacccg cgcgatagtc gtgcagtgcg
     5761 ctggatccct gctgcgatgg gcggttctat agcgatggtt cttgtcgtcg cggtaggcgt
     5821 tccgctggtc ggcggaccag caccagggcg aatctttgag cggacaacag cgacggagtg
     5881 ccgtgggtac cttgcgaagc tgaaggccgt gcccgatgaa cgtcttaggt ctgctgagat
     5941 ctcggtgaag tggatctggt cgtccgaccg ctacggctgg ggctgcgcgt tgagatacac
     6001 cgatggaagc accgggtctc tagtactccc aggcccgctg taagcgccga gcggcacttc
     6061 gctacttctg tggtcgagac acgggcagcc ctcgccgccg gaggactgcc gcacgcccga
     6121 acgcccagat ggggagaggg gcaatgcaga gtaggacgag ccgccaggcc gagcgatctt
     6181 ccaaccaagt ggcggcaact gccgccattg ccaaggcccc cactgctggg acccaccacg
     6241 cttgcgagtt tgcatcaacg gcccggaacc ccggccccgg cgctttagac catcggaggt
     6301 agatgtcgac cagggttgcc acacagaacc ccatgaaaag caggctggcc gcaaacctca
     6361 cccgaccact gtggcacagg cccattgaag gcgctcctat tgccgggtcg gcttgccgcc
     6421 cgaacggcac ttatgcgcca gcgatggcgt tgacttccac aagctcaacg tcgacgagtg
     6481 tcagcccaac ggctgcctgg ttcgacgcat ttgcctgcac gctggcctga aattcgcgag
     6541 cgtcggcttc tgcggagaac gcaacgagaa ccgtgctctt cgtgccatcg gtcgtccgtg
     6601 tccagattcc tgacacgaag ccgggcgcct cgcgtacccc tgggacgatg acgttgcgga
     6661 gcccgaactc ctgatcgtcc cgcctggctg gatcgacgtt gaaggttgac agcacggcgt
     6721 gagtggcttc attcggcatc cgcacagaat gccggttcgg cacgctcggt acggtcggtt
     6781 ccaaccgagc tgagggcgcg ccgccggctc tgccagggct ccgttccgtt gaaggagaac
     6841 cgatggcagc cacctcgatg ttgctctacg acccgcagca cgccgactcc gagatcgtca
     6901 ccgccgccgg cttcctcgcc ggctacagcg gccgaacccg cgaggcgtac agcctcgacc
     6961 tccgccagtt catcgcctgg tgccactccc acgagctgag cctcttcgag gtccagcgaa
     7021 cccacatcga gctgttcgcc cgagagctcg agatgcacgg gcgagctcga gcaacgatca
     7081 gccgccggct cgccacgatc gtcgggttct accgattcgc cgaggaggag ggcgtgatcg
     7141 cccactcccc ggcggtccac gttcggcgac cccgcctgac ctacgagtcc aacgccatcg
     7201 gcctcgaccg caacgagctc ggcgccctcc tcgtcgctgc cggcctcgcc gggccgaggg
     7261 accacgcgct ctgctcgctc ctcgccctga atgggctgcg cgtctcggag gcgatcggcg
     7321 cgctcatcga ggacctcgcc atcgaacgcg ggcaccggac cctcctcgtc caccgcaagg
     7381 ccggcaagac ggtccgcatc cctttggctc ctcgcaccgc cagagcagtg gacctgatcg
     7441 tgggagagcg gttcgagggg ccgatcttcg ctaacagcga aggccgggcg ctcgaccggc
     7501 actcggccgc ccgcatcgtc cgccgggtcg cgaagcgtgc cgagatcccg aagaagatcg
     7561 ggccgcactc cctacggcac gcgttcatca cggcagccct cgacgcgggc gtagccctcc
     7621 gcgatgttca agacgcggcg tcgcacgccg accccaggac gaccatgcgc tacgaccgag
     7681 gacgccaatc tctcgaccgc cacccgacct acgtcgttgc cgccttcgtc gccggcgccg
     7741 cacggtaacc cgctccataa cgcgctcgag gtggatgccg aaccggcatt atgcgcttgt
     7801 gaaggttcgg cgttttgtga ccgtggtcgg ggtcttcctg gttctggcgg cggcagcctt
     7861 ctcgtttcat cttgcgatgg agcgcgcaaa ggtcgatctc gccgaggccc cactgtgcga
     7921 ggtcggcgct gagttctctg gaatgtcatg ccgcgcagtg cgtagtggcg tcatattgga
     7981 ggcgtcacgg cgcgagttcg tggtgatggt cgatggacga cgtcgcctcg tcatggacgt
     8041 gacgctaaaa ggcacgatcc atgcgaagcc cggcgacgtt gtgtcgacgc aactctggga
     8101 ggggcagctg atgcgggtgg tggggactgg cttcgacgtt cgcagcaact cggcgcctga
     8161 gaatcggtcg ttcggcttcc gggtgatggc catcttcttt ctcgtgatgg cgttgccggg
     8221 tgggcttgcc gcaggattcg atcgccgctc ctaagcctcg atccaccgtt gtggatctga
     8281 ttgcggggct tcctggggtt gaggcttccg cgccgatcgc agctctcggt cgtcgaggtt
     8341 gggtcgcgtt gaagcaggcg ggtcttggtc ggttgagttc ctgacgccgt cgg
//
LOCUS       DATLCO010000302         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7759807, whole genome shotgun sequence.
ACCESSION   DATLCO010000302 DATLCO010000000
VERSION     DATLCO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7759807"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1124)
                     /locus_tag="VMZ33_06975"
     CDS             complement(189..1124)
                     /locus_tag="VMZ33_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUR17011.1"
                     /translation="MTIGLRVRGLATAIAARPRLTAFLAAVSISFSGVLYLYSDTSPE
                     TASFFRGLYGLPILFLAAFAERKTARPMNRKAVGWAIVAGVLFAGDLIFWHHTIEYVG
                     AGLATVLGNLQVVIVGLATWLLFGERLNRRTLIALPIMLVGVALIAGVLTGEAYGIDP
                     PLGVFLGILTAFSYAGYLIVIRRIGRGRLALPVAISTTSTTIVSLAVGVAIGTMQFGI
                     ALDQHFWLVLLGVSAQALGYLFISYSLPRLPAVVTSIILLTQPVIAVFLSIILVNETP
                     SVEQLGGVALVIGGIALATVTVRRPFRRSVAVTAP"
     gene            1158..2063
                     /locus_tag="VMZ33_06980"
     CDS             1158..2063
                     /locus_tag="VMZ33_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HUR17012.1"
                     /translation="MGRVEAPMNGKTPSLLLVGLLLAACSAGVPRTTTGPAPSTPPSA
                     TPATPTNPPGTNPPTAIAPIGSNPADPSGIDGRTFLSIGIKDGDQVRPLVPGTVIRIS
                     FNGNQISVQAGCNSFGGTYRLDGDTLVVDGGAMTEMGCDGPRHAQDDWIFGLIGSSPT
                     IALRGDDLVINAGQTTIEMKDREVVEPDQALVGPTWVLTSIITGQTVSSVPDNIVATI
                     KFNDDGSLEVHPGCNSGGGSYTVDGDRITFDEVFLTEMACMGAGGQVEATVLSVLDGA
                     QITFAIDAGSLTLMAGDLGLQFTAQ"
     gene            complement(2050..2361)
                     /locus_tag="VMZ33_06985"
     CDS             complement(2050..2361)
                     /locus_tag="VMZ33_06985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR17013.1"
                     /translation="MSTAEAKGLDITDDRQRGRYIAKQDGRFVGYTEYELEPGRIRFT
                     HTVVKPEFEGQGIGSRLARHVVGDAVSRDLQITPVCPFVRTWLERHPEFDENVDLPTE
                     P"
BASE COUNT          472 a          817 c          784 g          391 t
ORIGIN      
        1 ttcgcaccat ttcggcggtt cacgggggcg tcgttacgtc gccgagatgc ggttcgccag
       61 cgaagcctgt caacttgtaa cgtctgacat ggtcagacgg gcggtcaatt cgacgaattc
      121 atagccaatc gatcgttgac acggccgaca tccctggcgc gactacgcgg aacgggcgga
      181 gcagagggct acggagccgt gacggcgacc gatctccgga atgggcgacg tacggtgacg
      241 gttgccagcg cgatgccccc gatcaccaat gcaacaccgc cgagctgctc cacggacggc
      301 gtctcgttca ccaggatgat cgacaggaag acggcgatga ccggctgggt gagcaggatg
      361 atcgacgtca cgaccgccgg cagccgcggc agcgagtacg agatgaacag gtagcccagc
      421 gcttgggcgc tgaccccgag cagcacaagc cagaaatgct ggtcgagcgc aatgccgaac
      481 tgcatcgttc cgattgccac gccgacggcc agcgagacga tggtcgtcga cgtcgtggag
      541 atcgccaccg gcagcgccaa acggccgcgc ccgatccgcc ggatgacaat gagatagccg
      601 gcgtacgaga aggcggtcag gatgcccagg aaaacgccga gcggcgggtc gatgccatac
      661 gcctcgccgg tcagtacgcc cgcaatgagc gcgacgccga ccagcatgat cggcagcgcg
      721 atgagcgtgc gccggttgag gcgctccccg aacagcagcc aagtggccag acccacgatc
      781 accacctgga ggttgcccag cacggtggcg aggccggcgc cgacgtactc gatggtgtgg
      841 tgccagaaga tgaggtcacc cgcgaataga acgccggcga cgatggccca gccgacggct
      901 ttgcggttca tcggccgggc cgtctttcgc tcggcgaacg ccgccaggaa caggatcggc
      961 aggccataga ggccacggaa gaagctggcc gtctcggggg acgtgtcgga gtagaggtac
     1021 agcaccccgg agaacgagat ggacaccgcc gcgaggaacg cggtcaggcg cggacgggca
     1081 gctatcgcgg tggccagacc tctcactcgg aggccgattg tcacgcacga atccgaaccc
     1141 tgcggaaccc gatggccgtg ggtcgcgtcg aggcgcctat gaacggcaag accccttccc
     1201 tcctccttgt tggccttctt ctcgcggcct gcagcgccgg cgtaccgcga accacgacgg
     1261 gtccggcacc ctcgacgccg ccgagcgcca cgccggctac gccgacaaat ccgccaggta
     1321 ccaacccgcc caccgccatc gcgcccatag gcagcaaccc cgccgatccg tcgggtatcg
     1381 acggccgtac cttcctttcg atcgggatca aggacggcga tcaggtccgc cccttggttc
     1441 ccggcacggt catccgcatc agcttcaacg gcaaccagat ctccgtgcag gccggttgca
     1501 actcgttcgg cggcacgtac cgccttgatg gggacacgct tgtcgtcgac ggcggcgcga
     1561 tgaccgagat gggctgcgac ggccctcgac acgcgcagga cgactggatc ttcggcctca
     1621 tcggatcgag cccgacgatt gccctccgcg gcgacgacct cgtgatcaat gccggccaaa
     1681 caacaattga gatgaaggac cgcgaggtcg tcgagcccga tcaggccctc gtcggaccaa
     1741 cctgggtcct gacctcgatc atcaccggcc aaaccgtgtc gtcggtcccc gacaacatcg
     1801 tcgccaccat caagttcaat gacgacggct cactcgaagt tcaccccggc tgcaacagcg
     1861 gtggcggttc gtacaccgtc gatggcgaca ggatcacctt cgacgaggtt ttcctgaccg
     1921 agatggcgtg catgggcgcc ggcggtcagg tcgaggcgac ggttctctca gttctggacg
     1981 gcgcccagat cacgttcgcg atcgatgccg gctcgttgac gctgatggcc ggcgatctcg
     2041 gccttcagtt cacggctcag taggtaggtc gacgttctcg tcgaactcgg ggtgccgctc
     2101 cagccaggtt cgtacaaacg gacaaactgg agttatctgc aggtcgcggc tgacggcgtc
     2161 acccacaacg tggcgcgcca gccgcgaacc gatcccctgt ccttcgaact ccggctttac
     2221 caccgtgtgc gtgaagcgga tgcggccggg ctccagctcg tactcggtgt acccaacgaa
     2281 ccgtccgtcc tgcttggcga tgtagcggcc gcgttggcgg tcgtcggtta tgtcgaggcc
     2341 tttggcttca gctgtgctca ccactccagc gtatcctcgg ttcgaagaac ccgatgaccg
     2401 gaccgctttc aatcctgaat tgcgccagct gcgccaagcc gaacaagatc cgcccgagcg
     2461 agcg
//
LOCUS       DATLCO010000303         2022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5835649, whole genome shotgun sequence.
ACCESSION   DATLCO010000303 DATLCO010000000
VERSION     DATLCO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5835649"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VMZ33_06990"
     CDS             complement(<1..445)
                     /locus_tag="VMZ33_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR17014.1"
                     /translation="MLVVDDEAPITELLSTALRYMGYDVATARTGMAALESASRLPPD
                     LVVLDVMLPDIDGFEVCRRLRSDGDFVPVIFLTARDAEEDRVTGFIRGGDDYVTKPFS
                     LEELTLRISALLRRTKMTGAGAADSNRLRYRDLEMDEDRHQVLRDG"
     gene            complement(600..1460)
                     /locus_tag="VMZ33_06995"
     CDS             complement(600..1460)
                     /locus_tag="VMZ33_06995"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HUR17015.1"
                     /translation="MPLIQPRNIRDLIAPTVVVALDAWVDAGAASTTAARALADDSAP
                     TVATFESDVMYDYRSRRPTLEIMDGRPASLTWPDLSFRRTRIADRDILVLSGAEPDFR
                     WRELADDLASFARQLEVEQWISLGAIPAAVPHTRPVPVLGTESSPGLLRGGVAPGPQG
                     VLRVPSAAISVMDIALAEAGIPSVGYFAQIPHYISGDYAPAAIALIEALARHLDIEIA
                     VDALRAEADVLRTRLDTATALDEKTRAYVERLEGMVDEARLPEGDELISEIERFLRER
                     GSEPGHGQIH"
     gene            complement(1465..1884)
                     /locus_tag="VMZ33_07000"
     CDS             complement(1465..1884)
                     /locus_tag="VMZ33_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HUR17016.1"
                     /translation="MGDSLTVPDDLLDLVTTDRIGHVSFIRPDGSIVTHLMWIDWDGS
                     RIVTSSPVGSVKGDHARRDPHVSVSVSDPEDPWRFVIVRGYVTDIQPDEDLAFIDKMS
                     LRYSGTPYRWRTNEREVFYITPEHLRTGRGGWHRRKT"
BASE COUNT          365 a          663 c          664 g          330 t
ORIGIN      
        1 agccgtcgcg caagacctgg tgtcggtctt cgtccatctc caggtcgcgg tatcgaagcc
       61 ggttgctgtc ggccgcgccc gcgccggtca tcttcgtccg gcgcagcagc gcactgatcc
      121 ggagcgtcag ttcctccaaa gaaaagggct tcgtcacgta atcgtcaccg ccgcggatga
      181 agccggtgac gcgatcctcc tcggcgtcgc gcgcggtgag gaatatgacc ggcacgaagt
      241 cgccgtcgga gcgcagccgc cgacatacct cgaaaccgtc gatgtccggg agcatcacgt
      301 cgaggacgac gaggtcgggt ggcagcctgg aggcggactc gagggcagcc atgcccgtgc
      361 gcgccgtcgc gacgtcgtaa cccatatagc gcagggcggt ggagaggagc tcggtgatgg
      421 gcgcctcgtc gtcaacgacg agcacgcgtc cacgagattc ggcccccaag tcgggccgag
      481 gggacgcaga gatcgagttc atgtgctgta ttccacgccc ggaaccttag aggttgctgt
      541 gaaccagccg agaggacgct tggcttctcg cgcgatcccc tgcgcgatcc cctaaaggtt
      601 cagtggatct ggccgtgacc cggttcgctc ccgcgttcgc gcaggaaacg ctcaatctcg
      661 ctgatcagct cgtcaccttc gggcaaccgc gcctcatcga ccatcccctc gagacgctcc
      721 acgtaggccc gtgtcttctc gtcaagagcc gttgcggtgt cgaggcgcgt gcgcaaaacg
      781 tctgcctctg cccggagcgc gtcgaccgct atttcaatat ccaggtggcg cgccagcgcc
      841 tcgatcagcg caatggccgc cggcgcgtaa tcgccgctga tgtagtgcgg gatctgggcg
      901 aagtagccca ccgacgggat gccggcctca gcaagggcaa tgtccatcac cgagatcgcg
      961 gccgagggca cgcgcaacac gccctgtggg cctggcgcca cgccgccacg cagcagtccc
     1021 ggtgacgact cggtccccag gacgggcacc gggcgcgtat gaggcactgc tgccggtatc
     1081 gcgccgagcg agatccactg ctcaacctcc agctgtcgag cgaacgaggc caggtcgtcg
     1141 gccagctccc gccagcgaaa gtcgggttcg gcgccactca acacgaggat gtcgcggtcg
     1201 gcgatgcgag ttcgacggaa ggacaggtcg ggccatgtca atgaggccgg ccgcccgtcc
     1261 atgatctcca acgtcgggcg gcgcgagcgg tagtcgtaca tcacgtccga ctcaaatgtg
     1321 gccacggttg gtgcggagtc atcagccagc gccctggccg cggtcgtgga agccgcgcca
     1381 gcgtcaaccc acgcgtcgag tgccacgacc acggtcggtg cgatgaggtc gcggatgttc
     1441 cgcggttgaa tcaggggcac gccgctacgt ctttcggcga tgccagccgc cgcgaccggt
     1501 tctcaggtgc tccggcgtga tgtagaacac ctcgcgctcg ttggtccgcc agcgatacgg
     1561 cgtgcccgag tagcgcagtg acatcttgtc gatgaaggcc aggtcttcgt cgggctgaat
     1621 gtccgtgacg tacccgcgga cgattacgaa gcgccacgga tcctcggggt cggacaccga
     1681 gactgacaca tgcggatccc gccgtgcgtg gtcgcctttg acggaaccca cgggcgagct
     1741 cgtgacgatc cgagagccgt cccagtcgat ccacatgagg tgggtcacga tcgacccatc
     1801 gggccgaatg aacgacacgt gcccgatccg gtccgttgtc acgaggtcga gcaggtcatc
     1861 gggaacggtc agcgagtcac ccacccaaat acgataggcg agcacatgga actaggcctt
     1921 tacacgttcg gcgcagttca ccaggacccg actaccgggc agtcgataga cccccagcgc
     1981 cgtctcgagc acctggtcga ggagatggag ctggccgatc ag
//
LOCUS       DATLCO010000304         6704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7761352, whole genome shotgun sequence.
ACCESSION   DATLCO010000304 DATLCO010000000
VERSION     DATLCO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7761352"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1148
                     /locus_tag="VMZ33_07005"
     CDS             84..1148
                     /locus_tag="VMZ33_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HUR17017.1"
                     /translation="MYAVVLAGGGGTRLWPLSRRARPKPFLPLLGAESLFQLTLRRIE
                     PLIDGAATIVVSEQRHLPLVEQQAPQIPSRQRLGEPFGRNTAAAVALAAILSGRRDED
                     VMVVLPADHFIADEEGFRRTLGTAAQAAQDGSLVTLGIEPTGPETGYGYIVGASADEA
                     EVGVRRVERFVEKPPRERALELLASPNGAWWNAGIFVWRHDALLAGLAKYAPQIGNDI
                     RRGLDTGKSLTSIYEALPDISIDRALMEPASVEGKVKVVPADVGWSDLGSWDALHRAL
                     AARTRERDGVVAVGRSESVGSSNVLVHSTGDRLVVTIGLADTIVVDTPDVVLVCAADK
                     AQEVRAIVERLTQAKETEHL"
     gene            1145..2629
                     /locus_tag="VMZ33_07010"
     CDS             1145..2629
                     /locus_tag="VMZ33_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HUR17018.1"
                     /translation="MTDGSASAPLPPIVFGTDGWRARIGEDYTYENVRRCAQGVAEWA
                     RSSGTSQKGVVIAYDRRFASEFFAQAAAEVLLAYDIPVAMSQNAVPTQMTSYEVVERG
                     AACGIMITASHNPYPDNGFKVKSPSGAAASPDMLAVIEKTIRDYAGSQPPRQSYAEAV
                     ASGKVELYDPYPGYVEYTRRSLDLDRLKAADMRVLVEPMWGAGAGWISRLLAGGRIQI
                     TEIHQERNPFFGGVNPEPIRPNIDESLAILAAGGYDLGLLLDGDADRAGAVDEKGTFI
                     HQLQVMGLLMYYMLEHRGLRQPVVSTINETSMVGRLGEKYGVDVYETPVGFKYVGPKM
                     IETGAMMGGEESGGYGFGMHLPERDGIYADLLLLDLFVREREAGRWPVSAAVEHLHSI
                     AGPSFYLRNDVHVDKALYPALKDRLLVELVQGAPLSIAGHPVVRTDALDTKDGFKFWL
                     DEGSWLLVRFSGTEPLVRVYAEGSSAQLRDAILAEGSALVQGDK"
     gene            2626..3717
                     /locus_tag="VMZ33_07015"
     CDS             2626..3717
                     /locus_tag="VMZ33_07015"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HUR17019.1"
                     /translation="MTTTQEVSNLDDAGAVRRRDPGGMLGHAGAFGAQLRAGWEISRS
                     LTLGDLHRSAKSVAVLGMGGSAAAGDLVRGIFSDRLTVPIVAVRDYELPAWVGRETLV
                     VAASHSGSTEETISALGAALERRCPVIAMTTGGPIGDVAARVGLPRLVFPNETPPRAS
                     LGYTLMLLAGLLERAGMLTLDDAEIEAAVAAADAVAASCGPEKPTESNLAKQIAWSVL
                     DRLPIIEGSGFLAAVARRWKTQLNENSNSIAVAEELPEAMHNTVVGYDQPDALRDHQY
                     VLFLKGDADHARNAARAALSVELLDAVSIAHQTVVIEGDGRLAQAVTAISLGDYVSCY
                     LGLLYGMDPSATPVLTHVKQRMAELGQDD"
     gene            complement(3742..5136)
                     /locus_tag="VMZ33_07020"
     CDS             complement(3742..5136)
                     /locus_tag="VMZ33_07020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR17020.1"
                     /translation="MRDYASVSFWLETSGDDLTPRPSLGGSIEADVAILGAGYTGLWT
                     AYYLLRAEPSLRVVILERDIAGFGASGRNGAWCAPGMNISLSKLARLHGREAARQTYQ
                     QVSAAIDEIGSVADSEGMDIDWRRGGELIVARGGHESPSIQTAYRELEDFGFGDHYQV
                     LDAEATSARVAIGGATAALYTPDAAAIHPGKLARGLARVVERMGATIYEGTEVTDYQP
                     AGVLSRRPSFTTMAGEVTADVIVLAGEAYLTRLRRLHRQLIPIWSLIVLTEPLPDDVW
                     AEIGWRAHELIGSPRYTVVYLSRTTDGRLLFGGRGAPYRYGSPIREAYDRHDPTHEML
                     RANARAWFPQLRDARFSHAWGGPLGMARDWHPTISFDKRSRVATARGYVGHGVSNSNL
                     SGHTLAELITGQRTVRTELPLVNHRSRSWEPEPFRWIGVRFVQGALGRVDRLAEESGQ
                     PPRGRSLEEWLARH"
     gene            5385..5663
                     /locus_tag="VMZ33_07025"
     CDS             5385..5663
                     /locus_tag="VMZ33_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17021.1"
                     /translation="MIGKIEKANRYARELDRITFDRLSVTFRGDNDAHRVTLDSDRWQ
                     CNCHYFESWSTCAHVLALQKILGQMLPEGAQTSYFGAPEPVLAETVPA"
     gene            complement(5737..6285)
                     /locus_tag="VMZ33_07030"
     CDS             complement(5737..6285)
                     /locus_tag="VMZ33_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17022.1"
                     /translation="MKILAKTPMAMLLIVGAVASCSTGSRPTPSPTPLPPDPTPTPTA
                     PRANPQPQPPHGLVAVADMRVEGWAGSYCWQGTCADSPGIPPIDDLPEIESGDYPLTF
                     TLSDGATFTSWSASYGLDGRSLQPLDEGGLQVDPDANPGAEVPQLTRAEIANPPAGDW
                     VVSVFVRVDNGDLSYAWHVSVP"
     gene            complement(6296..>6704)
                     /locus_tag="VMZ33_07035"
     CDS             complement(6296..>6704)
                     /locus_tag="VMZ33_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17023.1"
                     /translation="NERTSLTLTSSTTSIPVGSGVQFTARLKIASDEDYPNLAGRALT
                     ERRVVLQRRALGATSWSNFTDLTSVADGTGRYVKTLTLTSTWDYRALFTAPSDEGLGG
                     SSSSVSRISVTTNDEYDCRPAGALWYRPLYEIC"
BASE COUNT         1121 a         2202 c         2272 g         1109 t
ORIGIN      
        1 gctcgcgtcc tgttctcgat aacgccgccc agcttctagc gacgaacagg ggccagccgg
       61 gccgacacat agaatggcca ggcatgtacg ccgtagtcct cgccggtgga ggcggcaccc
      121 gcctctggcc actgagccgg cgggcgcgcc ctaagccatt tctcccgttg ctcggcgctg
      181 agagcctctt ccagctgacg cttcggcgca tcgagccgct catcgacggc gcggcaacga
      241 tcgtcgtctc ggagcagcga cacctgccgt tggtcgagca gcaggccccg cagatcccgt
      301 cgcgccagag actgggcgag ccgttcggtc gtaacactgc ggcggccgtg gcactcgccg
      361 cgatcctgtc gggccgccgc gacgaagacg tgatggtcgt cctccccgcc gatcacttca
      421 tcgccgacga ggagggcttc cggcggaccc tcgggacggc cgcacaggcg gcccaggacg
      481 gctcgctggt cacgctcggc atcgagccga cgggtcctga aacgggttac ggctacatcg
      541 tcggggcgtc cgcggacgaa gcagaggtag gcgtgcgccg ggtcgaacgg ttcgtcgaga
      601 aaccaccacg cgagcgcgcc ctggagctcc tcgccagtcc aaacggcgct tggtggaacg
      661 cgggcatctt cgtctggcgc cacgacgcgc tgctcgctgg cttggccaaa tatgcgccgc
      721 agatcggcaa cgacatccgc cgcggcctcg acaccggcaa atcgcttacg tcgatctacg
      781 aggctctgcc cgatatctcg atcgatcgcg cgctgatgga gccggcttca gtcgagggca
      841 aagtcaaggt cgtcccggct gatgttgggt ggagcgatct cggcagctgg gacgccctcc
      901 atcgcgcgtt ggcggcgcgc acgcgcgagc gtgacggcgt tgtggctgtc ggacgctctg
      961 aatcggttgg atccagcaac gtcctcgttc actcgaccgg tgacaggctg gtcgtgacca
     1021 tcggtctggc cgataccatc gtcgtcgaca ccccggacgt cgtcctcgtc tgtgccgccg
     1081 ataaggcgca ggaagtgcgc gcgatcgtcg aacgcctgac ccaggcaaag gagaccgagc
     1141 acctgtgacc gacggcagcg cgagcgcgcc tcttccaccg atcgtcttcg gcacggacgg
     1201 ctggcgagcg cgcatcggcg aggactacac gtacgagaat gtgcgtcggt gcgcccaggg
     1261 cgtggccgag tgggcgagat cgagcgggac cagccagaag ggtgtcgtca tcgcctacga
     1321 ccggcgcttc gcgtcggagt tcttcgcgca ggcggcggca gaggtgctgc tcgcctacga
     1381 catccctgtg gcgatgtcgc agaacgcggt tccgacgcag atgacttctt acgaggtggt
     1441 cgagcgtggc gcggcgtgcg gaatcatgat caccgcctcg cacaacccgt acccggacaa
     1501 cggcttcaag gtgaagtcgc ccagcggcgc ggcagcgagc ccggacatgc tggcggtgat
     1561 cgagaagacg atccgcgact atgccgggtc gcagccgcca cggcagtcat acgcagaggc
     1621 agttgcctca ggcaaggttg agctctacga cccgtatccc gggtacgtcg agtacaccag
     1681 gcgatcgctg gacctcgatc ggctcaaggc ggccgacatg cgcgtgctag tcgaaccgat
     1741 gtggggcgcc ggcgcaggct ggataagtcg gctgcttgcg ggtggtcgga tccagatcac
     1801 cgagattcac caggagcgca acccgttctt cggtggcgtc aacccggagc ctatccggcc
     1861 caacatcgac gagtccctgg ccattctggc cgccggtggc tacgacctgg gcttgctgct
     1921 cgacggcgac gccgaccgtg ccggtgcggt cgatgaaaag ggcaccttca tccaccagct
     1981 ccaggtcatg ggcctgctga tgtattacat gctcgaacac cgcggcctgc gccagccggt
     2041 ggtgtcgacg atcaacgaga cctcgatggt cggccggctg ggcgagaagt acggcgtcga
     2101 cgtgtatgaa acgcccgtcg gattcaagta cgtcggccca aagatgatcg agaccggcgc
     2161 aatgatgggc ggcgaggagt cgggcggcta cggcttcggc atgcacctgc ccgagcgcga
     2221 cggcatctac gccgacctat tgctgctcga cctcttcgtg cgcgagcgcg aggccggtcg
     2281 ctggccggtg tcggctgccg tcgagcactt gcactcgatc gcgggaccgt cgttctacct
     2341 gcgcaatgat gtccacgtcg acaaggcctt gtacccggcc ttgaaggacc gtcttctcgt
     2401 cgagcttgtt cagggagcac cgttgtcgat cgccggtcat cccgtcgtcc gcacggatgc
     2461 gctggacaca aaggatgggt tcaagttctg gctggacgaa ggctcgtggc tgctggtccg
     2521 gttcagcggc acggagcctc tggtgcgcgt ctacgccgag ggttcatcgg cgcagctgcg
     2581 cgacgcgatc ctggccgaag gttccgcctt ggtacagggc gacaagtgac gacgactcaa
     2641 gaggtcagca acctcgacga cgcgggtgcg gtgcgcaggc gcgatccggg cggaatgctc
     2701 gggcacgctg gcgccttcgg ggcacagctg cgcgcgggct gggagatcag ccgctcgctg
     2761 acgctgggcg acctccacag gtcggcgaag tccgtcgccg tgttggggat gggcggctca
     2821 gcggcagccg gtgacctggt gcgcggcata ttcagcgacc gacttaccgt gccgatcgtc
     2881 gccgtacgcg attacgaact cccggcatgg gtcggccgcg agacactcgt cgtcgcggct
     2941 tcgcacagcg gctccaccga ggaaaccatc agcgcactcg gcgcggcgct ggagcggcga
     3001 tgccctgtga tcgcaatgac gacgggcgga cccatcggcg acgtggccgc gcgtgtcggc
     3061 ctcccgcggc tggtcttccc gaacgagacc ccgccgcgag cctcgctcgg ctacaccctg
     3121 atgttgctcg ccgggctgct ggagcgggcc ggcatgctca ccctcgacga cgctgagatc
     3181 gaggcggccg ttgccgcagc cgacgccgtt gcggcatctt gcgggccgga gaagccgacg
     3241 gagtccaacc tcgcgaagca gatcgcctgg tcggttctcg accgcctgcc gatcatcgag
     3301 gggtccggct tcctggccgc tgtcgcgcgg cgctggaaga cacagctcaa cgagaactcc
     3361 aactcgattg cggttgccga ggagctgcca gaggccatgc acaacacggt ggtcgggtac
     3421 gaccagcccg acgccctgcg cgatcaccag tacgtcctgt tcctcaaggg tgacgccgat
     3481 cacgcccgga acgcagcccg tgccgcgctc tctgtggagc tcctggatgc ggtctcgatc
     3541 gcccaccaga cggtggtcat cgagggcgac ggcaggctcg ctcaggccgt gacggcaatc
     3601 tccctgggcg actacgtgag ctgctacctg gggctgttgt acggcatgga tccgagtgcg
     3661 actccggtcc tgacgcacgt taagcagcgg atggccgagc tgggccaaga cgactaaagg
     3721 cgggtgtccg agcgtcgcta gctagtggcg ggctagccac tcctctaagg agcggccgcg
     3781 cggcggctgc ccggactcct cggcaagccg gtcgacacga ccgagtgcgc cctgcacgaa
     3841 tcggaccccg atccagcgaa acggctcggg ctcccacgac cgtgaccggt ggttgacgag
     3901 cggcaactct gtccggacgg tccgttggcc ggtgatcagc tctgcgaggg tgtggcccga
     3961 gaggttgctg ttcgacacgc cgtggccgac gtagccgcgc gccgtcgcca cccgcgaccg
     4021 cttgtcaaag gagatcgtcg ggtgccagtc gcgtgccatg ccgagcggcc cgccccacgc
     4081 atggctgaag cgcgcgtcgc gcagctgcgg aaaccacgcg cgggcgtttg cgcgaagcat
     4141 ctcgtgcgtg ggatcgtggc ggtcgtacgc ctcgcggatc ggtgacccgt agcgatacgg
     4201 cgcgccacga ccgccgaaca gcaggcggcc gtcagtggtg cgcgagaggt agacaacggt
     4261 gtagcgcggg gaaccgatca gctcgtgggc gcgccagccg atctccgccc aaacatcgtc
     4321 gggcagtggc tccgtcagga cgatcagcga ccagatcggg atgagttgac ggtgcaggcg
     4381 gcgtaggcgc gtcaggtaag cctcgcccgc caggacgatg acgtctgcgg tgacctcgcc
     4441 ggccatcgtc gtgaagcttg ggcgtcggga cagcacgccg gcgggctggt agtcggtgac
     4501 ctcagtccct tcgtagatcg tggcgcccat gcgctcgacg acgcgcgcca gaccgcgggc
     4561 gagcttgccc ggatggatag cggcagcatc cggcgtgtag agcgcggccg tcgcgccgcc
     4621 gatggccacg cgcgcgctcg tcgcctccgc atcgaggacc tggtagtggt cgccaaaccc
     4681 gaaatcctca agctcgcggt aagcagtttg gatcgacggc gactcgtgtc cgccgcgtgc
     4741 cacgatgagc tcgccgccgc ggcgccaatc gatatccatc ccctccgaat ccgccacgct
     4801 gccgatttcg tcgatcgcgg ccgacacctg ctggtaagtc tggcgggcgg cttcccggcc
     4861 atgtaaacgg gcaagcttcg agagggagat gttcattccc ggggcgcacc acgcgccgtt
     4921 gcgacccgat gccccgaacc cggctatgtc gcgctccagg atcacgacgc gtaacgatgg
     4981 ttcggcacgc aacaggtagt aggcggtcca cagcccggtg taacccgcgc caaggatcgc
     5041 tacgtcggct tcgatcgagc cacccaggga agggcgtggt gtcagatcgt cgccggacgt
     5101 ctcaagccag aatgaaactg aggcgtaatc gcgcatcaat gcgcgcgaag catacggccg
     5161 catccagggc ctccgggcga cgtgccgcac ggtgagccag gtgcaaggga atgacccgga
     5221 attcgaccca gttgggactt tgctggggcc aggggaagtt gtagccttag cccatcatcg
     5281 ctcggtaacg ccggactcga gcggtctgtg cgacccgagc gctatacgcg ggtccacgcg
     5341 agcccacgcg tcccccgccg ggaggttgcc caatgcactc gagcgtgatc ggcaagatcg
     5401 agaaggccaa tcgctacgca cgggagctcg accggatcac ctttgatcga ctttccgtca
     5461 ccttccgtgg cgataacgat gctcaccgcg tcaccctcga ttccgaccgc tggcagtgca
     5521 actgtcatta cttcgagtcg tggagcactt gcgcccacgt gctggcgctg cagaagattc
     5581 tcggccagat gctgccggag ggcgcccaga ccagctactt cggcgcgcct gagccggtgc
     5641 ttgcggagac ggttccggcg taggtcgcca ccggtcgcag atcgcaggaa ggccggcccg
     5701 caagggccgg ccttctgcct tgcaacgccg ctcgcgtcac ggcacgctga cgtgccaggc
     5761 atacgaaaga tcaccgttgt cgacacggac gaaaacactg acgacccaat cgccggccgg
     5821 tgggttcgcg atctcggcgc gggtgagctg cgggacctcg gcgcccggat tcgcatccgg
     5881 gtcaacctgg agtccgcctt catcaagcgg ctggagactg cggccgtcga gcccgtagct
     5941 cgccgaccag gacgtgaatg tcgccccgtc cgacagcgtg aacgtcagcg gatagtcacc
     6001 gctttcaatc tccggcaggt cgtctatcgg cggaatgccg ggcgagtcgg cacacgtgcc
     6061 ctgccagcaa tacgaaccgg cccatccctc tacccgcatg tcggccaccg cgacgaggcc
     6121 atgcggcggt tgcggctgcg gattggcgcg tggcgcagtc ggcgtcgggg ttggatcagg
     6181 tggcagagga gtcggactgg gcgtcgggcg ggagcccgtc gagcagctgg caaccgcgcc
     6241 cacgatgagc agcattgcca ttggggtctt ggccaagatt ttcattggcg tcctcctaac
     6301 agatctcgta gagcggtctg taccagagtg cccctgccgg tctgcagtcg tactcgtcgt
     6361 tggtggtaac ggaaatccgc gacacgctcg acgacgatcc gccgagccct tcatcgctcg
     6421 gtgctgtgaa tagtgcgcgg taatcccacg tcgacgtcaa tgtgagtgtt ttgacgtatc
     6481 ggccggtccc atctgctacc gacgtcaggt cggtgaagtt cgaccagctc gttgcgccca
     6541 gtgcccggcg ttgcagcacg acccgccgct cggtcaaggc ccgtccagcg aggttcgggt
     6601 agtcctcgtc cgacgcaatc ttgagccggg ccgtgaattg gacccccgag ccaaccggga
     6661 tgctcgtcgt cgacgaggtc agcgtgaggc tggttcgctc attc
//
LOCUS       DATLCO010000305         3349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1378979, whole genome shotgun sequence.
ACCESSION   DATLCO010000305 DATLCO010000000
VERSION     DATLCO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378979"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /gene="prfA"
                     /locus_tag="VMZ33_07040"
     CDS             <1..1123
                     /gene="prfA"
                     /locus_tag="VMZ33_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HUR17024.1"
                     /translation="GGRPPRAMTLELRLDELEQRLLEIEAEWSNPEVSADHDRSRLLG
                     QEQAQLAPVVGDYRLLRRVREQLEAARRDRAAESDPEVREMAAALIDELELDEVRLTE
                     ALRVHLLPTDPNDERNVIIEIRAGVGGEEAALFAAELFRMYARYAERRRWKTDLITAS
                     ETGIGGFKEVIFEVHGAGAYSRLKYEAGPHRVQRVPETESGGRLHTSTATVAVLPEAD
                     EVEVQIDDKDLKIDVYRSQGPGGQSVNTTDSAVRITHLPSGLVVAIQDEKSQHKNKAK
                     ALAVLRARLLEMEQRKAHEAEAEVRRSMVGSGERSEKIRTYNYPQDRVTDHRINVSVH
                     NLPNVLDGDLDGLLDQLITIDQAQRLSELERTPTDGAAD"
     gene            1110..2030
                     /gene="prmC"
                     /locus_tag="VMZ33_07045"
     CDS             1110..2030
                     /gene="prmC"
                     /locus_tag="VMZ33_07045"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HUR17025.1"
                     /translation="MRPTETPATVGSALREAAARLTEAGSETPRLDAEVLLGHVLGFA
                     RATLLAYLDLALPPAQMSTFEGLIARRGTGEPVSYIRGFKEFYGLALAVDGRALIPRP
                     ETELVVNLALATIGRRLTESARPAGAGPYRVWDVGTGSGAIVVALAVESRRHRYDSDV
                     RFRATDASPDALAVAVENAVGHGVADLIDFSVADLADEAESGFDLVVANLPYVPTADI
                     PTLPVAASFEPVSALNGGPDGLDVIRRLGAQLPTVLVADGMALLEIGAGQADAVRVFV
                     DEGLPGWSIDIHPDLANVPRVVELTRSVAA"
     gene            2027..2653
                     /locus_tag="VMZ33_07050"
     CDS             2027..2653
                     /locus_tag="VMZ33_07050"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HUR17026.1"
                     /translation="MTPRISASDPRAIDEAVAALHRGELIAIPTETVYGLAVLPTTTG
                     LERLIAAKHRSAEKGVQLLIDSIDQATALADVTPAARALAARYWPGPLTIVLSRRTDV
                     EVPETLGGGRPTLGLRLPDHDVPRAIARALGPIAASSANVSGEPDATTADHVAGYFSD
                     EISLIVDDGPVRGGVPSTVVSCADAGQPPQVLREGAISAAEISAVAAD"
     gene            2663..>3349
                     /locus_tag="VMZ33_07055"
     CDS             2663..>3349
                     /locus_tag="VMZ33_07055"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HUR17027.1"
                     /translation="MSVETRSRIAWASLAEVDPDLWNAMQGERERQRWNIELIASENY
                     AFAAVLEAQGSWLTNKYAEGLPGRRYYGGCEFVDEAERLAQQRALDLFPGAEHVNVQP
                     HSGAQANMAAYISMLNMGDRVLGMNLAHGGHLTHGSPVNFSGKWFDIHAYGVREEDAR
                     IDYDALDKQAHEIRPKMIVAGASAYPRIIDFERLGRIARDVDALLFVDMAHIAGLVAA
                     GLHPSPFPHAD"
BASE COUNT          570 a         1120 c         1122 g          537 t
ORIGIN      
        1 cggcggccga ccaccacgcg caatgacact cgagctgcgc ctcgacgagc tcgagcagcg
       61 gctcctggag atcgaggccg agtggtccaa ccctgaggtt tcggccgatc acgaccgctc
      121 ccggctcctt ggccaggagc aagcccagct cgcacccgtt gttggcgact atcggctcct
      181 gcgccgcgtg cgcgagcagc tcgaggcagc gcggcgggac cgcgcggctg agtccgatcc
      241 ggaggtgcgc gagatggccg ccgcgctcat cgatgaactt gagcttgatg aggtacgcct
      301 gaccgaggca ctgcgggtcc acctgctgcc gaccgatccg aacgacgagc gcaacgtcat
      361 catcgagatc cgcgccggcg ttggcgggga ggaggcggcg ctcttcgctg ccgagctgtt
      421 caggatgtat gcccgctacg ccgagaggcg acgctggaag actgatctga tcacggccag
      481 cgagaccggc atcggcggtt tcaaggaggt catcttcgag gttcacggtg cgggtgccta
      541 cagccgactg aagtacgaag ccgggcctca tcgcgttcag cgcgttccgg agacggagtc
      601 gggcggacga ttgcacacgt cgacggcgac cgttgcggtc ctgcccgagg cggacgaggt
      661 cgaggtccaa atcgacgaca aggacctcaa gatcgacgtc taccgatcgc agggaccagg
      721 cggccagagc gttaacacca ccgactctgc ggtgcggatc acgcacctcc cgtcgggcct
      781 cgtggtcgct atccaggacg agaagagcca gcacaagaac aaggccaagg ccctggcggt
      841 gctccgtgcg cgtctcctcg agatggaaca gcgcaaggcg cacgaggctg aggcggaggt
      901 gcggcgttcc atggttggct ccggcgaacg ctccgagaag attcgcacct acaactaccc
      961 tcaggaccgt gtcaccgacc atcggataaa cgtgagcgtc cataacctgc ccaacgtcct
     1021 ggatggcgac ctggacgggc tgctcgacca gctcatcacc atcgatcagg cgcagcgact
     1081 gtccgagctg gagcggacgc cgaccgacgg tgcggccgac tgagacgccg gcgacggtcg
     1141 gtagcgcact gcgcgaggcg gcggcgcggc tgaccgaagc gggatcggag actccgcggc
     1201 tcgacgctga ggtgcttctc gggcacgtgc tcggcttcgc gcgcgcgacg ctactcgcat
     1261 acctggatct agccctgcca cctgcccaaa tgagcacgtt tgagggtctc attgcgcggc
     1321 gcgggacggg cgagcccgtg tcctacattc gcggtttcaa ggagttctac ggactcgcac
     1381 tggccgtgga cgggcgggcg ctgatcccgc ggcccgaaac cgagctggtt gtaaatctcg
     1441 ccctggccac catcggcagg cgtctcaccg agagtgcccg cccggccgga gccgggccgt
     1501 accgcgtgtg ggacgtgggc acgggcagcg gcgcgatcgt cgtcgccctt gcggtcgagt
     1561 cacgccgcca ccgctacgat tcggacgtgc gattccgcgc caccgacgca tctccggacg
     1621 cattggctgt ggccgtggaa aacgccgtcg gccacggagt agctgacctg atcgatttct
     1681 ctgtggccga tctcgcggac gaggcggaga gcgggtttga cctggtcgtt gcaaacctgc
     1741 cctacgtacc cacggccgac attccgacac tccccgtcgc cgccagcttc gagcccgtgt
     1801 cggcgctcaa cggtgggccc gacggcctcg acgtcatccg tcgtctgggg gcgcagctgc
     1861 ccacggtgct cgtggccgac ggcatggcgc tgctcgagat cggtgcgggt caggcagacg
     1921 cggttcgggt gttcgtcgac gaggggcttc caggctggtc gatcgacatt caccccgacc
     1981 tcgccaacgt gccccgtgtc gtcgagctga cgcggtcggt ggccgcgtga cgccgcgcat
     2041 ctccgcgagc gatccacggg cgatcgacga ggctgtcgcc gccctccatc gcggcgagct
     2101 catcgccatc cccactgaaa cggtctacgg cctcgcggtt ctgcccacga ccaccggcct
     2161 ggagcggctg atcgccgcga agcaccgctc agccgaaaag ggcgtccagc tgctgatcga
     2221 ctccatcgac caggcgacgg cgctggccga tgtgacgccc gccgcccgcg ctcttgcggc
     2281 tcggtactgg ccaggcccgt tgacgatcgt gctaagtcgc cgtaccgacg tggaagtccc
     2341 ggagactctc ggcggcgggc gcccaaccct tggcctgcgc ctgcccgacc atgacgtgcc
     2401 gcgcgccatc gctcgcgctc ttggcccaat cgctgcgagc tcagccaacg tgagtggcga
     2461 acccgatgcg acgaccgcgg atcacgtggc cggctatttc agcgacgaga tctcgttgat
     2521 cgtggacgac ggaccggtcc gtggcggcgt gccgtcgacc gtcgtgtcct gtgccgacgc
     2581 tgggcaacca ccgcaagtcc tccgcgaagg tgcaatctcg gcggctgaaa tctcggccgt
     2641 cgccgccgac taaactgact tcgtgagtgt cgaaacgagg tcgcgcattg catgggcgag
     2701 ccttgccgag gttgaccccg acctgtggaa cgcaatgcag ggcgagcggg agcgccagcg
     2761 ctggaacatc gagctgatcg cgagtgagaa ctacgcgttc gcggcagtcc ttgaggcgca
     2821 gggctcttgg ctgaccaaca agtacgccga gggcctgccc gggcgccgct actacggcgg
     2881 ctgcgagttc gtcgacgagg ctgagcgact ggcgcaacag cgggcacttg acctcttccc
     2941 aggtgctgag cacgtcaacg tgcaaccaca ctccggcgcg caggcgaaca tggccgcgta
     3001 tatctccatg ctcaacatgg gcgaccgggt actgggcatg aacctggcac atggcggaca
     3061 cctgacccac ggctcgccgg tgaacttctc gggcaaatgg ttcgacatcc atgcctatgg
     3121 ggttcgcgag gaagacgcac ggatcgatta cgacgccctc gacaagcagg cgcacgaaat
     3181 tcggccgaag atgatcgtgg ccggggcgag cgcctatccg cggatcatcg atttcgagcg
     3241 cctgggtcgc attgccaggg acgttgatgc gctgctgttc gtcgacatgg cccacatcgc
     3301 agggttagtt gcggccgggc ttcacccgag ccccttccca cacgccgat
//
LOCUS       DATLCO010000306         3248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5105833, whole genome shotgun sequence.
ACCESSION   DATLCO010000306 DATLCO010000000
VERSION     DATLCO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5105833"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VMZ33_07060"
     CDS             <1..382
                     /locus_tag="VMZ33_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17028.1"
                     /translation="VLLQRAAIGFAVAVILRSQLAGSIVGVMFFFGESIVRYMLIFLS
                     VGAGGLSGLGEGQLDNQWFQFLPITIGDYVVNAAPGSVNLQGSFENLLIKPVPLGLAL
                     PAVLVYLVVTVGLALLVFRRQEIA"
     gene            388..1527
                     /gene="tgt"
                     /locus_tag="VMZ33_07065"
     CDS             388..1527
                     /gene="tgt"
                     /locus_tag="VMZ33_07065"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HUR17029.1"
                     /translation="MTSPISFEVLKPAPSDGSTRARLGRLTTPHGTVETPVFMPVGTN
                     ATVKALDPDDLEEVGAQIILCNTYHLYLRPGHERVERLGGLHRFMAWNRPILTDSGGY
                     QVVSLGDMRSVDDDGVTFRSHLDGSTHRFTPEHAIAVQEALGSDIAVCLDQPVPPHSS
                     TRASVAEAMARTHVWADRCLSAHKRHDQALFGIIQGGLEADLRAQSTLAITGMPFDGI
                     CIGGLAGDETAAQRRAALDVCVPLLENDPRPRYLMGLGSPLDLLDAVDAGIDMFDSVL
                     PARIARNGTVLVPEGKLNLRNSRFLDDGAPIQEGCRCRACRTFSRAYLAHLLRADELL
                     FYRLATLHNLTYMTDYMARIRVALAAGDFPRLVRESARSVASVSA"
     gene            1612..1779
                     /locus_tag="VMZ33_07070"
     CDS             1612..1779
                     /locus_tag="VMZ33_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17030.1"
                     /translation="MGSRDRPGREVKKKPKAKDNKPQLQSLLDSPPQSVEIIKPKRKP
                     RHENEPDANEE"
     gene            1822..2910
                     /gene="recA"
                     /locus_tag="VMZ33_07075"
     CDS             1822..2910
                     /gene="recA"
                     /locus_tag="VMZ33_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HUR17031.1"
                     /translation="MTIQGTVKVAGIEGNGVEKLDRAATERTRAVDAAILSIEKQFGR
                     GSIMKLGTSERQAVDAIPTGSIALDLAIGVGGIPRGRITEIFGPESSGKTTLCQHVLA
                     EAQKRGGVVAFIDVEHALDPAYARACGVNVDELLVSQPDTGEQALEITETLIRSNGVE
                     CVVVDSVAALVPRAEIEGEMGDSSMGMQARLMSQALRKLTGAVSRSNTALVFTNQLRE
                     KIGVMFGNPETTPGGRAMKFYASVRLDIRRIETIKSGTDSIGSRVRVKVVKNKVAAPF
                     RVAEFDVMYGEGISREGGLLDVGVASDILAKTGAWFNYGDLRLGQGREQAKEFLKSNA
                     DVCLKLETEIRAKVATIKVPVEGISEAE"
     gene            complement(2960..>3248)
                     /locus_tag="VMZ33_07080"
     CDS             complement(2960..>3248)
                     /locus_tag="VMZ33_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17032.1"
                     /translation="AARSTSVRDPQRGNLPQLPDLIGREVEAVVPRSDITKPVVELSA
                     DDCPDVGRGTGALQATQDGARLRLVLVGIGLDVNPDRIVTKQNFGDLAVQL"
BASE COUNT          575 a         1027 c         1059 g          587 t
ORIGIN      
        1 cgttctcctg cagcgcgccg cgatcggttt cgcggtcgcg gtgatcctgc gcagccagct
       61 ggccggttcg atcgtcggcg tcatgttctt cttcggcgaa tcgatcgtgc gctacatgct
      121 gatcttcctg tccgttggcg ctggtggttt gagcggactc ggggaagggc agctcgacaa
      181 ccagtggttc cagttcctgc cgatcacgat cggtgactat gtcgtcaacg cggctccggg
      241 ctccgtgaac ctgcagggat cttttgagaa cctcctgata aagccggtcc cgctgggcct
      301 cgcgttacct gcggttctcg tctacctggt cgtgaccgtc gggctcgcgc tgctcgtctt
      361 ccgtcgccaa gagattgcgt agttcacgtg acctccccaa tctctttcga ggtcctgaag
      421 cccgcgccca gcgacgggtc gacgcgggcg cggctgggtc ggctgaccac gccgcacggc
      481 acggtggaga caccggtctt catgcccgtc ggcaccaatg ccacggtaaa ggcgcttgat
      541 cccgatgacc tcgaggaggt tggcgcccag atcatcctgt gcaacaccta tcacctctac
      601 ctgcgcccgg gacacgagcg tgtcgagcgc ctcggcggcc tgcacagatt catggcctgg
      661 aatcggccga tcctgaccga ctcgggcggc taccaggtcg tgagcctggg cgacatgcga
      721 tccgtcgacg acgatggcgt caccttccgg tctcatctcg acggttcgac ccaccggttt
      781 acgcccgagc acgccatagc ggtccaggag gccctcggct cagacatcgc cgtgtgtctg
      841 gaccagcccg tcccgccgca ctcgtcgact cgcgcgtccg tggccgaggc gatggccagg
      901 acccatgtct gggctgatcg ctgcctatcg gcgcacaagc gtcacgacca ggcgctcttc
      961 ggcatcatcc agggtggctt ggaagcagac ctgcgcgctc agtcgacgct cgccatcaca
     1021 ggcatgcctt tcgacggcat ctgcatcggc ggcctggccg gtgacgagac cgcggctcaa
     1081 cgtcgtgcgg cactggacgt gtgcgttccg ctgctcgaga acgaccctcg cccgcgctac
     1141 ctcatgggcc tcggctcgcc actggacctg ctcgacgcgg ttgatgccgg catcgacatg
     1201 ttcgactcgg tcttgccggc gcgtatcgcc cgaaacggga cggtgctcgt tcccgagggc
     1261 aagctcaatc tccgcaactc gcgcttcctg gacgatggcg caccaattca ggagggctgt
     1321 cgctgccgtg cgtgtcggac attctcacgg gcatacctgg cgcacctgct gcgtgccgac
     1381 gagctgttgt tctaccggct ggcgacgctg cacaacctca cctatatgac cgactacatg
     1441 gcgcgcattc gcgtggcgct cgctgcgggc gacttcccta gacttgtgcg tgagtcggcg
     1501 agatcggttg cgagcgtgag cgcgtgacag acaacctggc acaaagttgg agtgaactgg
     1561 cagtgaagcg gtggcggcta gcgtgccgaa tacaccgcgg aggcgaacga aatgggatca
     1621 cgcgatcgcc caggccgcga ggtcaagaag aaaccgaagg cgaaggacaa caagccgcag
     1681 ctgcaatcgc tgctggactc gccgccgcag tcggtcgaaa taatcaagcc caagcgcaag
     1741 ccgcggcacg agaacgagcc ggacgcgaac gaggagtagt cctcggggtc gccggtcttc
     1801 ctatctagga gggattacca ggtgaccatt caggggaccg tcaaagtagc cggcatcgag
     1861 ggcaacggcg tggagaagtt ggaccgcgcc gctacggagc gtacgcgcgc cgtggatgcc
     1921 gcaatcctgt cgatcgagaa gcagttcggt cgcggctcga tcatgaagct cggcacgtct
     1981 gagcgacagg cagtggacgc cattcccacc ggctccatcg cgctcgatct tgcgatcggc
     2041 gtaggcggga ttccgcgcgg ccgaatcact gagatcttcg gcccggagtc gtcgggcaag
     2101 accacgctct gtcagcacgt ccttgcggag gcgcagaagc gcggcggcgt ggtcgcattc
     2161 atcgatgtcg agcacgctct ggatcctgca tatgcgcgtg cctgcggcgt caacgtcgac
     2221 gagctgctcg tcagccagcc ggacaccggt gagcaggcgc tggagatcac ggagacgctg
     2281 attcgctcaa acggtgtcga gtgcgtggtc gtcgactctg tggctgcgct cgtgccacgc
     2341 gccgagatcg agggtgagat gggcgattcg tccatgggca tgcaggcccg gctgatgagc
     2401 caggcgctgc gcaagctgac aggcgccgtc agccgctcga acaccgcgct ggtcttcacc
     2461 aaccagctgc gcgagaagat cggcgtcatg ttcggcaacc ccgagaccac tcctgggggc
     2521 cgcgcgatga agttctacgc cagcgtcagg ttggacatcc gccggatcga gacgatcaag
     2581 agcggcacgg actcaatcgg cagccgcgtg cgcgtgaagg tggtcaagaa caaagtggcg
     2641 gcacccttcc gcgtcgcgga gtttgacgtc atgtacggcg agggcatctc gcgcgaaggc
     2701 gggctgctgg acgttggcgt cgcttcagac atcctggcca agaccggtgc ctggtttaac
     2761 tacggcgatc tgcgcctcgg ccagggccgc gagcaggcca aggagttctt gaagtccaac
     2821 gccgacgtct gtctgaagct cgagaccgag attcgcgcca aggttgcgac gattaaggtt
     2881 cctgttgaag gaatatctga ggccgagtga caggcgcgag ccaaccagca ggtggaaggc
     2941 ccggcaggat gccgcatacc tagagctgca cggccagatc gccgaagttc tgtttcgtca
     3001 cgatccggtc gggattgacg tcgagtccaa taccgacgag tacgagccgg aggcgagcac
     3061 catcttgagt cgcctgcaaa gcgcccgtac cgcggccgac gtcaggacaa tcgtccgcgc
     3121 tgagttcgac cactggtttg gtgatgtcgg accttgggac gaccgcctcg acctcgcggc
     3181 caatgagatc tggcagctgt ggcaggtttc cccgttgcgg gtcacgcact gacgtggatc
     3241 tagccgcc
//
LOCUS       DATLCO010000307         4742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_1381411, whole genome shotgun sequence.
ACCESSION   DATLCO010000307 DATLCO010000000
VERSION     DATLCO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381411"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /gene="acnA"
                     /locus_tag="VMZ33_07085"
     CDS             <1..1681
                     /gene="acnA"
                     /locus_tag="VMZ33_07085"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HUR17033.1"
                     /translation="QTGRPDSTLQLVERYAKENGFWREPGVGPDFDDLLELDLATIEP
                     TVAGPRRPQDKVRLPELPENFRTAYPKTAERKPQTLTTKGIAATIDHGSVAIAAITSC
                     TNTSNPTVMIGAGLLARNALARGLDVKPTVKTSLAPGSRAVTEYYTAAGLMEPLEKLG
                     FGVVGYGCTTCIGNSGPLDVEVAEAVEANDLVVAAVLSGNRNFEGRIHPLARASYLAS
                     PPLVVAFALAGTVEIDLLSEPLGTGSDGQPVFLADIWPSPEEIKETIARAVSPDIFRR
                     IYSEVFDGDERWRSLPVPVGSKRYAWDTSSTYVARPPFLDGIQREAEPVSDIEGARVL
                     AMLGDSVTTDHISPAGTIPAWSPAGQWLQEHGVGPLEFNSYGARRGHHEVMMRGTFGN
                     IRLRNALTEKEGPYTTHQPSGDEMFIYDAAVRYAAENVPLVIICGREYGSGSSRDWAA
                     KGTKLLGVKAVLAESFERIHRANLVGMGVLPLQFLSGQNAQSLGLTGKEMFDVTGLAS
                     GLAQQQRVKLRFRDESGERFIEVISRLDGPVELDYYRQGGILPAVLRRLAH"
     gene            complement(1689..3011)
                     /locus_tag="VMZ33_07090"
     CDS             complement(1689..3011)
                     /locus_tag="VMZ33_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17034.1"
                     /translation="MTLRFQNALRSKLALAIAIAMLALPAINANAPVRAAVNSLGLRA
                     TYDVDATFNWATRAVSVRTAARVTNPGGSSVSTAAFNVVPLRQGNANLGAVTVNGSYA
                     PAQADDQTVLVTMSPALGPGDSATVVINYTATLNSTATGEQWLFARADGVMAAYRWIP
                     WLARETRYNRPSVGDPWATDISSSVTVDITTDRVLKFASTGRRISSSNGGLTQHFTAA
                     SVRDFNFTAAPDYVTRTRWANNTKITFYYRTMNATNVLNVAAGALTQFASKLGSAPYS
                     TLNIGEVGPSSRWGAIESPGHFWLPRGGSASIVNWEVAHEVAHEWFYASVGNDQAYEP
                     FADEAVADFLARDYLNQWASSQCAQNDLDHTVYSIGNCYPWVIYVQGNLYLKKYRADV
                     GSTDFWQGLRNYYQNNKFGFGGTRELLLTLDNAAGVNYNHEARFPSLF"
     gene            complement(3076..3151)
                     /locus_tag="VMZ33_07095"
     tRNA            complement(3076..3151)
                     /locus_tag="VMZ33_07095"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3116..3118),aa:Val,seq:cac)
     gene            complement(3241..3498)
                     /locus_tag="VMZ33_07100"
     CDS             complement(3241..3498)
                     /locus_tag="VMZ33_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HUR17035.1"
                     /translation="MDVPTFFRVFVQLLVTVLFLIVTGRVLMSWVNPRFEGPVARFLF
                     ETTEPMLSPIRRILPQSGMIDLSPMVLGFVLLILMRIVLLR"
     gene            complement(3522..4187)
                     /locus_tag="VMZ33_07105"
     CDS             complement(3522..4187)
                     /locus_tag="VMZ33_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HUR17036.1"
                     /translation="MLERIAAAARRAGRNETAVQLVAVSKGVSAGRLVAARSAGIRTF
                     GENRVQEAEAKIPVVAVSEWHLIGHLQSNKARRAVELFDVVESVDSAELATRLATLAL
                     ELRNGRPLPIYLQVNVDRVQSKAGLDPDDLPAALATIGALPALELRGLMTVGRLVDRP
                     EDARPTFRRLRELSESLSITNPRLGTGLSMGMSTDFEVAVEEGATVVRVGRALFGERP
                     NDR"
     gene            complement(4253..>4742)
                     /locus_tag="VMZ33_07110"
     CDS             complement(4253..>4742)
                     /locus_tag="VMZ33_07110"
                     /inference="COORDINATES: protein motif:HMM:NF023746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17037.1"
                     /translation="GLFEVDEAAEIIRSTADPEANIIFGTVVDERMGDEISITVIATG
                     FDASRKRDRADARTFTQAPAEAEVNVGAGLRVTREPIREIEQRDAVAVPVQSREGAAA
                     YAASEPNGNGYNLPSSNPSIGGLPPAAAVQPRSYDRVEPKRGPQTEIEDLDIPAFLRR
                     NR"
BASE COUNT          829 a         1529 c         1581 g          803 t
ORIGIN      
        1 ccaaacgggt cgacccgact ccaccctgca actggtcgag cgctatgcca aggaaaacgg
       61 cttctggcgc gagccaggag tagggcctga cttcgacgac cttctcgagc tggacctcgc
      121 gacgatcgag ccgaccgtcg ccggcccgcg gcggccgcag gacaaggtcc ggttgcccga
      181 gctacccgag aacttccgaa cggcttatcc gaagaccgcg gagcgcaagc cacagacgct
      241 gacgacgaag ggcatcgcgg ccacgatcga ccacgggtcc gtcgccatcg cggccatcac
      301 ctcatgcacc aacaccagca acccgacagt gatgatcggt gccggcctgc tggcccggaa
      361 cgcgctcgca cgcggactcg acgtcaagcc gacggtcaag accagccttg cccccggatc
      421 acgggccgtc acggagtact acacggctgc cggcttgatg gagccgctcg agaaactggg
      481 tttcggcgtc gtcggctatg gctgcacgac gtgcatcggc aacagcggac cactcgacgt
      541 tgaagttgcg gaggccgtgg aggcgaacga cctggtcgtc gccgccgtcc tgtcgggcaa
      601 tcgcaacttc gaggggcgca tccatccgct ggctcgcgcc agctacctcg cgtctccccc
      661 actcgtcgtt gcgttcgcgc tggcggggac cgtcgagatc gacctcctgt cggagccact
      721 cggcaccggc agcgacggtc agccggtatt cctcgccgat atctggccct ccccggagga
      781 aatcaaggag accatcgcca gggcggtctc gccggacatc tttcgtcgta tctacagcga
      841 ggtgtttgac ggagacgagc gctggcgcag cctgcctgtc ccggtcggta gcaagcgcta
      901 cgcatgggac acgagctcaa cgtacgtagc acgaccgccg ttcctcgacg gcattcagcg
      961 cgaagccgaa cccgtgagcg acatcgaggg cgcccgcgtc ctggcgatgc tcggcgactc
     1021 ggtcacaacc gatcacatct cgccggcggg cacgatcccg gcctggagtc ctgccgggca
     1081 gtggctgcag gaacatggcg tcgggcccct ggagttcaac tcgtacggcg cgcgccgcgg
     1141 ccatcacgag gtgatgatgc gtggcacgtt cggcaacatc cggctacgca acgctctgac
     1201 cgagaaagag ggtccgtaca cgacccacca gcccagtggc gacgagatgt tcatctacga
     1261 cgcggcggtg cgttacgcgg ctgaaaacgt gccgctggta atcatctgcg gccgtgagta
     1321 cggcagtggg tcatcccggg actgggcggc caagggcacg aagctgctgg gcgtcaaggc
     1381 tgtgctggct gagagcttcg agcgcatcca tcgcgccaat ctcgtcggca tgggcgtgct
     1441 gccgctccag tttctgtccg gacagaacgc tcaatctctg ggcctcactg gcaaggagat
     1501 gttcgacgtc accggcctcg cgagcggtct ggcccagcag cagcgcgtca agctccgctt
     1561 tcgggacgag tccggtgagc gcttcatcga ggtgatcagc cgcctcgacg gacccgtgga
     1621 gctcgactac taccgccagg gtggcatcct ccctgccgta ctgcggcggc tggcccactg
     1681 acgattgttc agaaaaggct ggggaagcgg gcctcgtggt tgtaattgac cccagccgcg
     1741 ttatccagcg tcagcagcag ctctcgcgtt ccaccgaaac cgaacttgtt gttctggtag
     1801 tagttgcgca ggccctgcca aaagtcagtt gacccgacgt cagcccggta cttcttgaga
     1861 tacaggttgc cctggacgta gatcacccac ggatagcaat ttccgatgct gtagacggtg
     1921 tgatcgagat cgttctgggc acactgcgac gaggcccact ggttgaggta gtcacgcgcc
     1981 aaaaagtcgg ccacggcttc gtccgcgaac ggctcgtacg cctggtcgtt gccgacggat
     2041 gcgtagaacc actcgtgagc aacctcgtgc gcgacctccc agttgacgat cgaggccgag
     2101 ccgccgcgcg gcagccagaa gtgacccggc gattcgattg ctccccagcg agaggatgga
     2161 ccgacctcgc cgatgttcag agtcgagtac ggcgccgacc cgagcttgct ggcgaactgc
     2221 gtcagcgccc cggccgccac gttgagcaca ttcgtggcgt tcatggtccg gtagtagaaa
     2281 gttatcttgg tgttgttcgc ccaacgggtc cgcgtcacgt agtcgggggc ggccgtgaag
     2341 ttgaagtcac ggacgctggc ggcagtgaaa tgctgggtca ggccgccgtt ggaggacgag
     2401 atgcggcggc ccgtgctggc gaacttgagg acccgatcgg tcgtgatgtc cacggtgacc
     2461 gacgagctga tatccgtcgc ccatgggtcg ccgacactgg ggcggttgta tcgcgtctca
     2521 cgcgcaagcc acgggatcca gcggtaggcg gccatcacgc catcggcgcg tgcaaacagc
     2581 cactgctcgc cggtcgccgt ggagttgagc gtggccgtgt agttgatgac gactgtcgcg
     2641 gagtcacctg ggccgagtgc tggggacatc gtcacgagca ccgtctggtc gtcggcctgc
     2701 gcgggcgcgt agctgccgtt gactgtcacg gcacccaggt tggcgttccc ctgacggagt
     2761 ggcacgacgt tgaaggcggc ggtagacacc gacgagccgc cggggttggt aacgcgtgca
     2821 gcggtccgga cgctcacggc acgggtggcc caattgaagg tggcgtcgac gtcgtaggta
     2881 gcccgcaggc cgagactgtt caccgctgcg cggactggcg cgttggcgtt tattgccggc
     2941 agggcgagca tggcgatggc aatcgcaagc gcgagcttcg atcgcaatgc gttctggaat
     3001 cggagcgtca tgttccccct cggactggcg tctctcacgt ctgtcggatc atccgcggat
     3061 aacggcacgg agatttggtg ggcgatactg gattcgaacc agtgaccccg cggatgtgaa
     3121 ccgcgtgctc taaccactga gccaatcgcc cccaagcgtc cgacggacga tcgtcattat
     3181 ccgcccgcgg agccagtcgc gccagttgcg cgtcgagccg gtgttggcgc tcggcgccgg
     3241 tcagcgcaac agcacgatgc gcatcaggat cagcagcacg aagccgagga ccatcggcga
     3301 gaggtcgatc atcccgctct gcggcaggat ccgccggata ggcgacagca tcggctcggt
     3361 cgtttcgaac aggaagcgcg ccacgggtcc ctcgaaccgc gggttgaccc agctcatgag
     3421 cacccgaccg gtgacgatga ggaagagcac cgtcacgagc agctggacga acacgcggaa
     3481 gaaagtgggg acgtccaact tggcccggag tatacgaggc gctaacggtc gttcggtcgc
     3541 tctccgaaaa gggcccggcc gaccctgacg actgttgcgc cctcctccac cgccacctcg
     3601 aagtctgtgg acatgcccat ggacagtccg gtgcccaggc gcggatttgt gatgctcagg
     3661 gattccgata gctcgcgcag ccgacggaag gtcgggcgcg cgtcctctgg ccgatcaacc
     3721 agccgcccca cggtcatcaa accgcgcagc tccagggctg gcaaggcacc gatcgtcgca
     3781 agcgctgctg gtaggtcgtc gggatcaagc cctgccttcg actgaactcg atcgacgttg
     3841 acctggagat atatcggcag cggccggccg ttacgcagct cgagcgccag agtcgcgagg
     3901 cgggtggcta gctcagcaga atcgaccgac tcgacgacgt cgaacagctc gacggcgcgg
     3961 cgtgccttgt tcgattggag atggccgatg aggtgccact ctgagaccgc tacgaccggg
     4021 atcttggcct cggcctcctg gacgcggttc tcgccgaatg tccgaatgcc cgcgctgcgg
     4081 gcggcgacga ggcggcccgc cgaaacgcct tttgagacgg caacgagctg gaccgctgtc
     4141 tcattgcggc ccgcgcggcg tgcggcagca gctatccgct cgagcacacg cgtgtgggct
     4201 tcctggaaag cgacgacctc gccgcgcccg ggttgattcg ctgtggcggg gctcagcggt
     4261 tccggcgcag gaacgccggg atgtcgaggt cctcgatctc cgtctgtggc ccgcgcttgg
     4321 gctcgacacg gtcgtagctg cgcggctgaa cggccgcggc cggcggcagg ccaccgatcg
     4381 acgggttgct cgacggtagg ttgtacccat tcccgttcgg ctccgatgcg gcataagccg
     4441 cagcgccttc gcgtgactgg acgggcacgg caactgcgtc gcgctgttcg atctcgcgga
     4501 tgggctcgcg cgtgacacgc agcccggcac cgacgttgac ctcagcttcg gcgggagcct
     4561 gggtgaaggt ccgcgcatca gctcggtcgc gcttccgcga ggcatcaaac ccggtggcga
     4621 tgacggtgat cgagatctcg tcgcccatgc gctcgtccac gaccgtgccg aagatgatgt
     4681 tggcctccgg gtcggccgtg gagcggatga tctccgccgc ctcgtcgacc tcgaacaggc
     4741 cg
//
LOCUS       DATLCO010000308         4233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5110306, whole genome shotgun sequence.
ACCESSION   DATLCO010000308 DATLCO010000000
VERSION     DATLCO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5110306"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..236
                     /locus_tag="VMZ33_07115"
     CDS             30..236
                     /locus_tag="VMZ33_07115"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HUR17038.1"
                     /translation="MGNDDQFEGKFEQARGDVKENLGDAMGNEQMENEGKLDQAKGNV
                     REGLGDVREGIDDAVDSVRDKDDR"
     gene            complement(331..1083)
                     /locus_tag="VMZ33_07120"
     CDS             complement(331..1083)
                     /locus_tag="VMZ33_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HUR17039.1"
                     /translation="MLVGLIPYLLLVMLPTLVITLWAAWRVRSTYGKWDKVDSGLQLS
                     AFDFARYLLDRNNLKHVQVQPVAGHLTDHYDPRSKTLRVSQAVSGAAQFGQYDPQSRS
                     VGYAEPTTRRLSVAAAAVIAHEVGHALQDAQGDKAMAVRQAIVPLAQIGSGVAPWLII
                     AGLFMNIFGLSVVGVIFFGAAVLFTFVTLPVEFGASNRALAFVQPLGLIGERGEGARQ
                     VLRAAGWTYVAAALTAILTFLYYLWLVFGARR"
     gene            1300..1683
                     /locus_tag="VMZ33_07125"
     CDS             1300..1683
                     /locus_tag="VMZ33_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17040.1"
                     /translation="MTNENRDQQGQSGTDWQGGSGEAAQGGSDGSQDQSSGTGGSDWS
                     TGSGAGQESDYTSGSGGGQGGGGGQGGESGMGESGMSGGGSISPGDTGSGITSGGGDQ
                     GSGTSEWGEGGDSGSGGQEDRGQGM"
     gene            1799..2704
                     /locus_tag="VMZ33_07130"
     CDS             1799..2704
                     /locus_tag="VMZ33_07130"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HUR17041.1"
                     /translation="MTCSRGAVMFQTFSRSLELAKASLSVLRSDKELLVFPLISFIAL
                     VVVTISFAVPLWLTGAVADAVDGKINIPGAILAFLFYLVSYTVTFFFNTALVGAAMIR
                     LDGGDPTLRDGFRIAMSRLPQIIAYALIAATVGMILRWISERAGFVGQIVAGIVGFGW
                     SVATFLVVPILVLENVGPVDAIKRSASLLRKTWGEQLVGNFGIGLIFGLLALVALVIG
                     VALIVAIAQVSTILAGVAVVVTALIVGAIALVGAAASGIFTASLYRYATKGDPGPAFR
                     TETISAAFRPGDGAPAGVATFGGPR"
     gene            3046..3861
                     /locus_tag="VMZ33_07135"
     CDS             3046..3861
                     /locus_tag="VMZ33_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUR17042.1"
                     /translation="MKKRFQAAGLDVGRGRVCRTTKQVRRFIDEVGFPVVAKPNIGVG
                     AAKTYKLESEADLERYLVDKPEADFIIEEFITGELITYDGLTGRQGEIVFDSTFHYSS
                     GVMEAVNAQLDLFYWIPRVIPEDVQATGRAMARAFDLRERPFHFEIFRTADGRLIPLE
                     VNMRPPGGWTVDMFNYANEFDLYREWANVVVHGKFDAIITRPYNVVFVSRRDFRQYAL
                     SHEDVMRKFGHLVVQEGRLERVIATAMGDFGYIMRSPELPELFEATHHIQQTV"
     gene            3872..>4233
                     /locus_tag="VMZ33_07140"
     CDS             3872..>4233
                     /locus_tag="VMZ33_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUR17043.1"
                     /translation="MRREHRYWVSGNLGQDMELLVYGHSGLPLICFPSHNGRLRDWEA
                     FGMVDAVADLIEAGRLLMISVDGVDWQSWTNKAVPVEQRARRHNDYHRYITDEVVPFV
                     RTHSGRDQAWVTGCSMGG"
BASE COUNT          761 a         1331 c         1392 g          749 t
ORIGIN      
        1 ggtcctgacc gtgcattgga ggtcctttaa tgggtaacga cgatcagttt gaaggcaagt
       61 tcgagcaggc tcgcggcgac gtcaaagaga acctcggaga cgccatgggc aacgagcaga
      121 tggagaacga gggcaagctc gatcaggcaa agggcaacgt tcgcgagggt ctgggtgatg
      181 tccgtgaggg catcgacgac gccgttgaca gtgtccgcga caaggacgac cgctagtcaa
      241 tcatcagttc gcgagtaaat gagagccggg ccgcaaggcc cggctctttc tttgtgtctg
      301 cggcgagccg cgaaggcctg gcggcgtgcg ctagcggcgt gcgccgaata cgagccacag
      361 gtagtagagg aacgtcagga tcgccgtcag agcagctgct acgtaggtcc aacccgcggc
      421 acgcaggacc tgcctggcgc cctcgccccg ctcgccgatg aggcccaatg gctgcacgaa
      481 ggccagcgca cggttgctcg cgccgaactc cacggggagg gtcacgaacg tgaatagcac
      541 cgcggcaccg aagaagatca cgccgacgac cgacagtccg aagatgttca tgaacagccc
      601 ggcaatgatc agccacgggg cgacgcctga gccgatctgc gccagcggca cgattgcctg
      661 gcgaacggcc atggccttgt cgccctgcgc gtcctgcaaa gcgtggccga cctcgtgggc
      721 aatcaccgcc gcggccgcga cggacaggcg cctggtggtg ggctcggcgt agccgaccga
      781 gcggctttgg ggatcgtact ggccgaactg cgctgcgcct gagactgcct ggctgacgcg
      841 cagcgtcttg gagcgtgggt cgtaatgatc ggtcaggtgg ccggcaaccg gctggacctg
      901 aacgtgtttc aggttgtttc tatcaaggag gtagcgcgcg aaatcgaagg cggacagctg
      961 cagaccggag tcgaccttgt cccatttgcc gtatgtagaa cgcactctcc aggccgccca
     1021 cagcgttatt accagcgtag gcagcatgac caggagcaag tagggaatca gcccgaccag
     1081 catctcgttt tttgaacctc caaggaatgg gagccggcct taatggtacg cgcgttcggt
     1141 tcggatcccg cctggtgcgg agtcgcgcag atgttgcact gctgttagat cgtgcgccgc
     1201 agcctgattc ccgtcggcta cgcggagcgg tgcatcgtgg tcggcagcaa cgctggccgc
     1261 tcatctcgcg gccggagcag ttaagacatg gaggtctcca tgacgaacga gaatcgcgac
     1321 cagcagggcc agagcggcac cgattggcag ggtggcagtg gcgaggccgc gcaaggaggt
     1381 tccgacggga gccaggacca gtcgtccggt accggcggct cggattggag caccggctcc
     1441 ggcgccggcc aggagagcga ctacacgtcg ggcagcggtg gcggccaggg tggcggtggc
     1501 ggccagggcg gtgagtccgg catgggcgag tcgggcatga gcggcggcgg ttcgatcagc
     1561 ccaggggaca caggctcggg catcaccagc ggaggcggtg accagggctc aggcacctcc
     1621 gaatggggcg agggcggcga cagcggctcc ggcggccagg aggaccgcgg tcagggcatg
     1681 tagccccaaa cgccggtaac cacataacgc agccaatgcg actactggcc gggcgctcag
     1741 gcgtccggcc ttttcctatc ggttacgctc tgcccccacc gtcatcccgt cacatgccgt
     1801 gacatgcagc aggggcgccg tcatgttcca gaccttcagc cgcagcctgg agctagccaa
     1861 agcgagcctg agcgtgctgc gctcggacaa ggagctcctc gtctttccgc ttatctcgtt
     1921 catcgcgctg gtggtcgtga cgatcagctt cgccgtgcca ctatggctga ccggcgccgt
     1981 cgcggatgct gttgatggga agatcaacat ccccggggcg atcctcgcgt tcctgttcta
     2041 cctcgtctca tataccgtca cgttcttctt caacaccgcg ctcgtcggcg ccgcgatgat
     2101 tcgcctggac ggcggtgacc caacgctccg cgacggcttc cgcatcgcca tgtcgcggct
     2161 tccccagatc atcgcctacg ccctaatcgc cgcgacggta ggcatgatcc tgcgctggat
     2221 cagcgagcga gctgggttcg ttgggcagat cgtggccggg atcgttggct ttggctggag
     2281 cgtcgccacc ttcctcgtcg tgccgatcct cgtgcttgag aacgttggcc cggttgatgc
     2341 catcaagcgc agcgcctcgc tgctgcgcaa gacgtggggc gagcagcttg tcggcaactt
     2401 cggcatcgga ctgatcttcg gcttgctggc actcgtggca ctggtgatcg gcgtcgcgct
     2461 catcgtcgcc atagcccagg tatcgaccat cctcgcgggc gtggccgtcg tcgtaacggc
     2521 gctcatcgtg ggcgccatcg ccctcgtcgg tgcggccgcg agcggcattt tcactgcgag
     2581 cctgtatcgg tatgcgacga agggcgaccc gggccctgcg tttcgcactg agacgatctc
     2641 tgccgctttc cggcccgggg acggtgctcc ggccggtgtc gcgacctttg gtggtccgcg
     2701 ctgaacgttg tcttcatatc gccgcacttt ccaccgaatt ggtggcggtt cgtccgcggt
     2761 ctcgcagagg tcggcgcgac cacgctcggc atcgccgatg cgaactggga ggacctggag
     2821 ccggagcttc gtgagtcgct ggacgactat taccgcgtcg acgacttagg caattacgac
     2881 cagctgacgc gtgccctcgg gtacttcaca catcgccacg gacggatcgg ccgcctggat
     2941 tctctgaacg agcactggct ggagacagag gcacgcctgc gcaccgactt caacatccgt
     3001 ggcataaaca ggaacacgat cgggccgatc aagcgcaagt cactgatgaa gaaacgcttc
     3061 caggctgccg gactggacgt tggccgtggc cgcgtctgcc ggacaacgaa gcaagtgcgg
     3121 cgctttatcg acgaggtcgg cttcccagtc gtggccaagc ccaatatcgg cgtcggcgcc
     3181 gccaagacct acaagttgga gtcggaagcc gacctggagc gctacctggt cgacaagcct
     3241 gaggccgact tcatcatcga ggagttcatc accggcgagc tgatcaccta cgacgggctg
     3301 acagggcggc agggcgagat cgtcttcgac tcgacattcc actattcgtc gggcgtcatg
     3361 gaggcggtca acgcacagct cgatctcttc tactggatcc cgcgcgtcat ccccgaggat
     3421 gtccaggcga ccggtcgcgc catggcacgc gcattcgacc tccgcgagcg accgttccac
     3481 ttcgagatct tccgaacagc ggacggcagg ctgatcccgc tcgaggtgaa catgcgtccg
     3541 cccggtggct ggaccgtcga catgttcaac tacgccaacg agttcgacct ctaccgcgaa
     3601 tgggccaatg tggtcgtcca cgggaagttc gacgcgatca tcacccggcc ctacaacgtg
     3661 gtcttcgtga gccgccgtga cttccgtcag tacgcgctgt cgcacgagga cgtcatgcgc
     3721 aagttcggac atctcgtcgt ccaggagggc cggctcgaac gggtcatcgc gacggcgatg
     3781 ggggacttcg gctacatcat gcgcagcccc gagctgccgg agctattcga ggccacccat
     3841 cacatccagc agactgtcta ggctcgacct ggtgcgacgc gaacatcggt actgggtcag
     3901 cgggaacctc ggccaagata tggagctcct ggtctacggc cactcaggtc tcccgttgat
     3961 ctgcttcccg tcgcacaacg gacggctgcg cgactgggaa gccttcggga tggtcgatgc
     4021 cgtggccgac ctcatcgagg ccgggcgcct tctgatgatc tctgtcgacg gggtcgactg
     4081 gcagagctgg accaataagg cagtccccgt ggagcaacgc gcgcgccgac acaacgacta
     4141 ccaccgctac atcaccgacg aggtcgtgcc gttcgtccga acccattcgg gccgtgacca
     4201 ggcctgggtg accggctgca gcatgggcgg ttt
//
LOCUS       DATLCO010000309         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5117544, whole genome shotgun sequence.
ACCESSION   DATLCO010000309 DATLCO010000000
VERSION     DATLCO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5117544"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VMZ33_07145"
     CDS             complement(<1..1161)
                     /locus_tag="VMZ33_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HUR17044.1"
                     /translation="MTDLVLGRKPPPRARLGVRFAVFGLIVVVVVGLLTTRLFYLQVV
                     EGGYYAGLATENGRALIPLRSTRGLIYDRAGRQLAVNIPSYVVRIRPADLPFSQRDGV
                     VSRLSSLLNVPEADLIETLDRQANNSFELVRLANDVPDDVARIITEESGSLPGVQVSV
                     EERRQYDYGPLVAHVMGYTGSVTADELEAFGDLGYLNDDQIGKTGVEATFEEILRGTY
                     GEQEVEQDALGRILRTTEVTREPDPGNSIELTIDVEIQREAEEALKWATDIVNLQRGV
                     VIVMNPQTGEILALVSLPNYDNNLFASGISGADYQALLEDPNRPLVNFGLSEQYPPGS
                     TYKLVTGSGALEDGIIKDSTLVETAGYIEIGTYRYYDWNRRGFGPLDIYGGFA"
     gene            complement(1158..1664)
                     /locus_tag="VMZ33_07150"
     CDS             complement(1158..1664)
                     /locus_tag="VMZ33_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17045.1"
                     /translation="MTLPFSALVAVIAALLETSVLAELPLAGATIDLVLVCAAVATLV
                     LGVEDGLAIAFLGGLLVDMVVPDRPMGAATLAILLALGLAFVVSRAMGPGRRLIVVGV
                     ILILTPVYHVLFTGIMALVANADLAIDISTILIAAVLNAVLAIPIAALFGALERRFGA
                     TERAAWYP"
     gene            1934..>2496
                     /locus_tag="VMZ33_07155"
     CDS             1934..>2496
                     /locus_tag="VMZ33_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR17046.1"
                     /translation="MGEVIACANQKGGVGKTTTVINLAAYLALAGRHVLVIDLDPQGN
                     ATSGLGYDRSQPEGSIYAALLGREPTPNLIVDTQIDGLSLIPSSGDLAGAEIELIALA
                     QRERRLQQALNGVREKFDFVLLDCPPAVGLLTVNALTAADGVLVPIQTEYYALEGLSQ
                     LLSTINLVRDNLNPRLRLAGVLLTMYDA"
BASE COUNT          483 a          760 c          817 g          436 t
ORIGIN      
        1 ggcgaagccg ccgtagatgt ccagcggtcc gaaaccacgt cggttccagt cgtaatacct
       61 gtacgtgccg atctcaatgt agccagcggt ctccacgagc gtggagtctt tgatgatccc
      121 gtcctcgagc gcgcccgaac ccgtgacgag cttgtaggtc gatcccggtg ggtactgctc
      181 ggagaggccg aagttcacga gcgggcggtt cggatcctcg agcagcgcct ggtaatcagc
      241 gcccgagatg ccgctggcga acaggttgtt gtcgtagttg ggcagtgaca cgagggcgag
      301 gatctcgccg gtttgcgggt tcatgacgat gacgacgccg cgctgcaggt tgacgatgtc
      361 ggtggcccac ttcagcgcct cctctgcctc acgctggatt tccacgtcga tggtgagctc
      421 gatagagttg cccggatcgg gctcgcgcgt gacctcggtc gtgcgcagga ttcggccgag
      481 cgcatcctgc tcgacctcct gttcgccgta cgtgccgcgc aggatctcct cgaacgtggc
      541 ctcgacaccg gtcttgccga tctggtcgtc gttgagatag ccgagatcgc cgaaggcctc
      601 caactcgtcg gcggtcaccg agccggtata gcccatgacg tgcgcgacga ggggtccgta
      661 gtcgtactgc cgccgctctt cgacgctgac ctggaccccg gggagggagc cactttcctc
      721 ggtgatgatc cgggcgacgt cgtcaggcac atcgttggca agccgcacaa gctcgaagct
      781 gttgttcgcc tgacggtcga gcgtttcgat taggtccgcc tcgggcacgt tgagcagcga
      841 ggagaggcga ctgacgacac cgtcgcgttg gctgaagggc agatcggccg gccgaatccg
      901 aacaacgtac gaggggatgt tgacggccag ctggcgtccc gcgcggtcgt agatgaggcc
      961 gcgcgtcgag cgcaacggga tcagggcgcg gccattctcc gttgcgaggc cggcgtagta
     1021 gccaccttca acgacctgga ggtagaagag gcgcgtcgtc aggaggccaa cgaccactac
     1081 gacgatcagg ccgaagacag cgaagcggac gccgagtcgc gcacgtggcg ggggcttgcg
     1141 gcccaggacg aggtcggtca tggataccag gcggcacgct cggtcgcgcc gaagcgacgc
     1201 tccaaagcac cgaatagggc agcgatcggt atggcgagta cggcgttgag caccgcagca
     1261 ataagaatcg tgctgatgtc gatcgcgagg tcggcgttgg ccacaagcgc catgatcccg
     1321 gtgaagagca cgtgatagac cggcgtcagg atcaggatca cgcccacgac aatcaggcgg
     1381 cgcccaggtc ccatcgcacg tgatacgaca aatgcgaggc ccagcgcgag caggatcgcg
     1441 agcgtggcgg cgcccatcgg tcgatcgggg accaccatgt ccacgaggag tccgccgaga
     1501 aaggcaatgg ccagaccgtc ttccacaccc aacacaaggg tggcgacggc agcgcagacc
     1561 aaaacaaggt cgatggtcgc gcccgcgagc ggcagctcag cgagcacgct cgtttccagg
     1621 agcgcggcaa tgaccgctac gagcgcggaa aaaggcagag tcaagaaggg tctccggtgg
     1681 cgcctcggag tataggcgcg gtccctgtga gcccattcag gcggtgaaat cgcacagatg
     1741 ttcgaaattg ttcgtcgtga aacagcgacg attgccagtt ggtggtgttt tgtgcttcac
     1801 gtgaaacatt ccaatcggta gggctcggag gaagtgcccg ataaggcgct gatgagggac
     1861 cgtgccgaca ctccgggatc gggtgggtcc gatagaatca atcccagtga atgcgcgttt
     1921 ctccggccgt tgattgggcg aggtcatcgc ctgcgcgaat cagaagggtg gcgtcggcaa
     1981 gacgacaacc gttatcaatc tggcggcgta tctcgcactc gcagggcggc acgtcctggt
     2041 catcgacctt gatcctcagg gcaacgccac gagcgggctc ggatacgacc gttcgcagcc
     2101 cgaaggctcg atttatgcag ccctccttgg ccgtgaaccg actccgaatc tcatcgtcga
     2161 cacgcagatc gacgggctat cgctcatccc ttcgagcggc gacctcgccg gtgccgaaat
     2221 tgaactcatt gcgctcgccc agcgcgaacg gcgtcttcag caggccctca acggcgtgcg
     2281 ggagaaattc gatttcgtgc tcctcgactg ccctccggca gtggggctcc tcaccgtcaa
     2341 cgcgctcaca gctgccgacg gtgtgctggt acccatccaa accgagtact acgcattgga
     2401 ggggctcagt cagctgcttt caaccatcaa cctcgtccgc gacaacctca atccccggct
     2461 acgcctggca ggcgtgctgc tgacgatgta cgacgc
//
LOCUS       DATLCO010000310         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7038702, whole genome shotgun sequence.
ACCESSION   DATLCO010000310 DATLCO010000000
VERSION     DATLCO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7038702"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="VMZ33_07160"
     CDS             <1..1713
                     /locus_tag="VMZ33_07160"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HUR17047.1"
                     /translation="NKDLWVTCALGPYNFEFMDQVHREIVTKYRVDGIFTNRWAPQAE
                     CHCLHCQRNFKTATGLELPKSTDASDPGRRAFVEWRKARLTELWKVWDATIRAANPEA
                     RLIPNGPPDLKTANELAEIQFTDYQARRGVTPPWANGRHGKEYRAVMGRRPVGGIFSV
                     GLEEAYRWKDSVQSEPEMRLWVAEGTANGMRPWFTKFSGTLYDRRWLPVVERIFNWHA
                     QHERYLRNELPLARVALVLSEQTAAVHPGVANGDRSADHVLGMYHALVEARVPFEMVH
                     EAFLTPDRLDRYKLVILADAAALSDAQCAALEAYVKRGGSLLATFASSLYDEQGRRRA
                     DFGLANLFGVSFAGRVDGPMQNAYLSLDADPSTGRRHPLLDGLEGTPRIVNGVFRLEV
                     TPKIDFPSPLTLIPTYPDLPMEDVYPRTPHTDIREVYLRDLGASRVAYVPWDIDRTFW
                     DVLCVDHGRLLKNLVTWTLNEPPPVDVSGPGLVDVTIWRQRDSLTVHLVNLTNPMMMK
                     GPLREVLPIGPLTVRIQLPAGLRARKVHLLTAGIEPRVQSAGTSIVVTVPSVEVHEVV
                     AIDT"
     gene            complement(1898..>2414)
                     /locus_tag="VMZ33_07165"
     CDS             complement(1898..>2414)
                     /locus_tag="VMZ33_07165"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HUR17048.1"
                     /translation="AERLWLIPAAVPESSIHRMPADAVDLAAAADAYAEELSGALGRV
                     AVLDLILLGMGPDGHVASLFPGHQLLTEHDRSVAAVSDAPKPPPRRLTLTLPVLTRAR
                     SVVVAAFGAPKAKALGQALGDPASALPVAQVLRAASRAIVLADDPAGVELRRAGSGNP
                     YPGFAPSPTLP"
BASE COUNT          398 a          821 c          830 g          365 t
ORIGIN      
        1 aacaaggact tgtgggtgac ctgcgcgctg ggtccctaca acttcgagtt catggatcag
       61 gtccaccgcg agatcgtcac gaagtacaga gtcgacggga tcttcacgaa ccgctgggcg
      121 ccgcaggcag agtgccactg cctgcactgc cagcggaact tcaagacggc aaccgggctg
      181 gagctgccga agtcgacgga cgcgtcggat ccggggcgac gcgcgttcgt cgagtggcgc
      241 aaggcgcggc tgacggagtt gtggaaggtg tgggatgcga ccatccgtgc cgccaacccc
      301 gaggcgcgac tgatcccgaa cggcccaccc gatctcaaga ccgccaacga gctggccgag
      361 atccagttca ccgactatca ggcgcggcgc ggcgtcaccc cgccgtgggc caacggccgt
      421 cacggcaagg aataccgtgc ggtcatgggc cggcgtccgg tcgggggcat cttcagcgtc
      481 ggcctcgaag aggcctatcg ctggaaggac tcggtgcaga gcgagccgga gatgcggctc
      541 tgggtcgcgg aaggcaccgc caatgggatg cggccgtggt tcacgaagtt ctcgggcacg
      601 ctctacgacc gccgctggct gccggtcgtc gagcggatct tcaactggca cgcgcagcac
      661 gagcgctacc tccgcaacga gctgccgctc gcgcgcgtcg ccctggtact gtccgagcag
      721 accgcggccg tgcatccagg cgtggcgaac ggcgatcgat ccgcggacca tgtgctgggg
      781 atgtatcacg cgctggtcga agcacgcgtg cccttcgaga tggtccacga ggcgttcctc
      841 acgcctgacc gtctggatcg ctacaagctg gtgatcctgg ctgacgccgc ggccctctcc
      901 gacgcgcagt gcgccgcgct cgaggcctac gtcaaacggg gcggcagcct cctggccacc
      961 ttcgcctcgt ccctctacga cgagcagggc aggcggcgag ccgatttcgg cctcgccaac
     1021 ctgttcggcg tgtcgttcgc cggtcgcgtc gatggcccga tgcagaacgc gtatctgagt
     1081 ctcgacgcgg atccctcgac gggccggcgg catccgcttc tggacggcct cgaaggcacg
     1141 ccgcgcatcg tgaacggcgt gttccgtctc gaggtgacgc cgaagatcga tttcccgtca
     1201 ccgctgacgc tcatcccgac ctacccggac ctgccgatgg aggacgtcta cccgcggacc
     1261 ccgcataccg acattcgcga ggtctacctc cgcgatctcg gcgcgagccg tgttgcctac
     1321 gtgccatggg acatcgatcg cacgttctgg gatgtcctct gcgtggacca cggccgcctg
     1381 ctcaagaatc tggtcacgtg gacgctgaac gagccgccgc cggtggacgt ctcgggccca
     1441 ggcctggtcg acgtcaccat ctggcgccag cgcgactcgc tgaccgtgca cctcgtcaac
     1501 ctcaccaacc cgatgatgat gaagggcccg ctgcgcgagg tcctgccgat cgggccgcta
     1561 accgtgcgga ttcagctgcc ggccggcctg cgcgcccgga aggtgcacct gctgaccgcc
     1621 ggtatcgaac ctcgcgtcca gtccgccggt acctcgatcg tcgtcaccgt gccgtcggtc
     1681 gaggtgcacg aagtcgtagc gatcgataca taggtccttc aggtcgggcg tgaaggagca
     1741 gagagctcac ggctgacagc tgctacccag acgaggcctc gtaccggagc cagacctttg
     1801 cgtgaccaac ggtcgctcag ttctgccgca gcgcgccgat tggatcgatg cgcgcggccc
     1861 gcgtcgcggg aaggactgtc acgcttccct aagatttcta aggaagcgtt ggcgaaggcg
     1921 caaatcccgg gtacgggttg cccgaccccg cccgtcgcag ctcgacgcca gcaggatcgt
     1981 cagccaggac gatcgcccgt gacgcggcac gcagcacctg cgcgactggc agcgcagagg
     2041 ccgggtcgcc gagcgcctga ccgagcgcct tggccttcgg cgcgccgaac gcggcaacga
     2101 cgacggagcg ggcgcgggtg aggacgggga gggtgagggt gagccgccgg ggcggcggct
     2161 tgggggcgtc gctcaccgcc gcgaccgacc gatcgtgctc ggtcagcagc tgatgaccgg
     2221 gaaagagcga ggcgacgtgc ccatccgggc ccatcccgag gaggatgaga tcgaggaccg
     2281 ccacacgccc gagcgcgccg ctcagctcct cggcgtacgc gtcggcggcg gccgcgaggt
     2341 ccacggcgtc cgccggcatc cggtggatgg aggactcggg caccgcggcg gggatcagcc
     2401 agaggcgctc cgca
//
LOCUS       DATLCO010000311         4364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8528552, whole genome shotgun sequence.
ACCESSION   DATLCO010000311 DATLCO010000000
VERSION     DATLCO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8528552"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2630)
                     /locus_tag="VMZ33_07170"
     CDS             complement(<1..2630)
                     /locus_tag="VMZ33_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR17049.1"
                     /translation="MSFAQTSGPATLIGRRDEQNELKSVVTDRDAPMLARLIDGPPGA
                     GKTALLGYVLQEATRSGAVVMRMAPAAAESSLPYAGLGDLFDGVSAERLADLPAAQQR
                     ALGAALTHGTAADESNSHAVARAVVSVLRSIAERELLVVAVDDLQWLDSASSRALLFA
                     MRRLADVRVRFVLTRRPDEATDWWDALRRALPEHPRWRIPLGPLTVDEVAALLGARLD
                     LRLPRPTIVTIHRRSEGIPLFALELGRAVLRGRQQGSGAADQTEDLTSLLAGRLLPLG
                     APARAAVLATSLASQPTFRMLVSIAGKSGVEQAINAEVLQRRDDRIRLVHPLFGSAAE
                     SLASTVDRAVIHTKLAGLVPDEEEAALHRARGASDPSAATSADLEMAAGRAVHRGAPE
                     MAAELAEHAARLTPADDSVARTRRLVVMADQLLAAGDPQRARAVLEELAAGMSRGPER
                     ADVLWRLADSVGDDLALSTRLSEQALAEAGDNAELKTSVRLALGVFTWLSGDMWASLE
                     HVREAARLAVECRNDQLTAIALGEVMHAEAVLGLDTDPAVYDRALALEATMASFPPAM
                     RPTFQKAVVCIYTDRLDEARPLLNAELMRAEASGDESARVAVLFRMSELELRAGNWAL
                     ATRQADECLALATQAGIEQEQCVALDASAAVSAHLGRVHEATTAAEQALAIATGNGDE
                     ISVLRSRGVLGFVSLSVGDLRAADHWLAPGLATIERLDIGELSIYGVVQNQLDVLIGM
                     GRHDAAQVLIELLRRKAGPQRRAWHLAVAARGHAMLAGARGNVEEALAQIALALAEHS
                     RLPQPFEYGRTLLAKGSIERRHGKRTTARATLAEALEVFDNLGAALWAEAAAAELARI
                     SGRTPASGSLTDAETRVASLV"
     gene            2737..3144
                     /locus_tag="VMZ33_07175"
     CDS             2737..3144
                     /locus_tag="VMZ33_07175"
                     /inference="COORDINATES: protein motif:HMM:NF045421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17050.1"
                     /translation="MATGSGTKDDPWQLKTPPGTSEYTMYRDDTADPPLIVCMVGSTK
                     LTYLARAVDDLHAMLRSHGGWVDLGAADEQKPATPGTVEGWGRAGDNPLGGWYGQRKG
                     YRGRFGMYMPPLMEALGLAELTHEPRGNKIRAL"
     gene            complement(3141..3797)
                     /locus_tag="VMZ33_07180"
     CDS             complement(3141..3797)
                     /locus_tag="VMZ33_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17051.1"
                     /translation="MSDWDRIEPVGPARAGVLWLHWFAPQEGGDDKTQFRAEAESLAS
                     EGIASALPQLSFPWKLDPSGSSADAQAIEAELADLSDAVEWLARHSDRLAIVGHDFGA
                     MYALLLMARDPRWGSGVVIAAANRWADWFVRFWKIDEDRLEYMRALRPLDPIEHVGSI
                     APRPLLMQFANDDYFIAGMDAAELYLAAGEPRRLERYDADHAMHSEAARADRRQFLLG
                     "
     gene            complement(3794..>4364)
                     /locus_tag="VMZ33_07185"
     CDS             complement(3794..>4364)
                     /locus_tag="VMZ33_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HUR17052.1"
                     /translation="KMGKSEKGAVMLDPSMTSPYDFYQYWLNDDDSLAVQHLRWLTLM
                     TADQVADIERQQSESPERRPAQRALAYDLTARIHGNAEADRQVRVADAAFAGGPINDP
                     EVLDALFGAIDHFEFGTGDAANALDLAAVSGLATSRSEARRLITQGGMSINDVRVTDI
                     EASLPEPVAGRYFVLRAGRKRLLIARLRG"
BASE COUNT          740 a         1492 c         1454 g          678 t
ORIGIN      
        1 acgaggctcg ccactcgtgt ttcggcgtcg gtcagcgacc cagaggccgg tgttcggccg
       61 ctgatgcgcg ccagttcggc ggcggccgcc tccgcccaga gcgccgcgcc gaggttgtcg
      121 aagacctcga gcgcttcagc aagcgttgcc cgcgccgtgg tgcgcttgcc gtgccggcgc
      181 tcgattgaac ccttggccag cagggtgcga ccgtattcga acggctgcgg cagacgagag
      241 tgctccgcaa gcgccaacgc tatctgggcc aaggcttctt ccacattgcc acgggcaccg
      301 gcaagcatgg cgtggccgcg cgcggcgact gcgaggtgcc acgcccgccg ctgtgggccg
      361 gcctttcgac gtaggagctc gatgagaact tgcgccgcgt cgtggcgacc catcccgata
      421 agcacatcga gctggttttg aacgaccccg tagatcgaca gctctccaat gtcgagacgc
      481 tcgatcgtgg cgaggcccgg cgccagccag tggtcggcgg cgcgaaggtc acccacggac
      541 aggctcacga aaccgaggac gccccgcgac cggagcacgc ttatctcgtc gccgttgccg
      601 gtcgcgatgg ccagcgcttg ctccgccgcc gtagtggcct cgtggacacg gccgaggtgg
      661 gccgaaacag cggccgacgc gtccagggct acgcattgct cctgttcgat ccccgcctga
      721 gtggccaatg ccaggcactc gtctgcctgt cgagtggcaa gggcccagtt cccggctcgc
      781 agctccagct ccgacatgcg gaagaggacg gcgactcggg cactctcgtc accgcttgcc
      841 tcggcccgca tgagctccgc gttgagaagt gggcgcgcct cgtcgaggcg atccgtgtag
      901 atgcagacga cagccttctg gaaggtcggt cgcatcgctg gcgggaatga cgccatggtc
      961 gcctcgaggg ccagcgcgcg gtcgtagaca gccggatctg tgtccagccc gagaacggcc
     1021 tcagcgtgca tcacctcacc gagcgcgatg gcagtgagct ggtcgttgcg gcattcaacg
     1081 gccaacctcg ccgcctcccg aacgtgctcg aggctcgccc acatgtcacc cgacagccac
     1141 gtgaagacgc caagcgcgag acgaactgac gtcttcagct cggcgttgtc accggcttcg
     1201 gccagcgcct gctcgctgag ccgcgttgag agcgccaggt cgtcgcccac ggagtcggcc
     1261 aggcgccaca ggacatcggc acgttcaggc ccgcggctca tccccgcggc gagttcctcg
     1321 agaaccgctc gcgcgcgctg cggatcgccc gctgcaagca gctgatcggc catgaccacg
     1381 agacgccggg tgcgtgcgac cgaatcatcg gctggcgtca gccgcgcggc gtgctcagcg
     1441 agctcggcgg ccatctcagg cgcgccgcgg tggaccgccc gccccgccgc catctcgagg
     1501 tcggccgaag tggcggccga aggatcggac gcaccgcggg cccgatgcag agcagcttcc
     1561 tcctcgtcgg gaacgagtcc ggcgagcttg gtgtgaatca ccgcccgatc aacggtcgac
     1621 gccaaagact cggcggctga gccaaacagt gggtgcacga gtcggatccg gtcgtcgcga
     1681 cgctggagca cctcagcgtt gatggcctgc tcaacgcccg acttgccggc gattgagaca
     1741 agcatgcgga acgttggttg cgatgccagg gacgtggcga gaactgctgc gcgtgccggc
     1801 gcgccgagcg gcaggagcct tccggccagg agactcgtaa ggtcctctgt ctgatctgcg
     1861 gcgcccgagc cttgctggcg tccgcgcaga acggcgcgcc cgagctcgag cgcgaagaga
     1921 ggaatccctt ccgaacggcg gtgaatggtc acgattgtcg gccgtggcag gcgaagatcg
     1981 agacgtgcac cgagcaaagc tgcaacctca tctacggtga gcggcccgag cgggatccgc
     2041 cagcgcgggt gctcaggcag cgcccggcgg agtgcgtccc accagtcggt agcctcgtct
     2101 ggtcgccgcg tcaatacgaa acggacacgg acgtctgcca agcgtcgcat ggcaaacaac
     2161 agcgcccgcg acgaggcgga gtcaagccat tgcaggtcgt cgacagcaac cacgagcagc
     2221 tcgcgttccg cgatggagcg gagcaccgat acgaccgctc ttgcgacagc gtggctattg
     2281 ctttcgtcag cggcggtccc gtgagtaagg gctgcgccga gcgctcgctg ctgcgcggcg
     2341 ggcaagtcgg ccaaccgctc cgcggacaca ccgtcgaaaa ggtcacccag accagcgtat
     2401 ggaagggatg actccgcggc ggctggtgcc atcctcatga cgacggcacc tgagcgagtc
     2461 gcctcctgga gcacatagcc aagcagcgcc gtcttgccgg cacccggcgg cccgtcgatc
     2521 aagcgggcca gcatgggcgc gtcgcgatcg gtgacgactg atttgagctc gttctgctcg
     2581 tcgcgccttc cgatgagagt ggcaggtccg ctcgtttggg cgaagctcaa cctgtcagtg
     2641 gtcctctcct cgcgttgcga cccccgcgtc gtacgttatg aacttggtcg cccgtctctt
     2701 gataatgcgg cgaccagctt ctgaggagga cgatacatgg caaccggcag cggcaccaag
     2761 gacgatccgt ggcagctcaa gacgccaccg ggtacctccg agtacacgat gtatcgcgac
     2821 gacacggctg atccgccgct catcgtgtgc atggtcggct cgaccaaact gacctaccta
     2881 gcacgggcgg tcgacgacct gcacgccatg ctcaggtccc acggcgggtg ggtggacctc
     2941 ggcgcagccg atgagcagaa gccggccacg ccagggaccg tcgagggctg gggacgtgct
     3001 ggcgacaacc cacttggcgg ctggtacggc cagcgcaagg gttatcgcgg acggttcggg
     3061 atgtacatgc cgccgctgat ggaggccctt ggcctggccg agctcacgca cgagccgcgc
     3121 ggcaacaaga ttcgggcgct ctagccgagc agaaactgcc ggcggtctgc gcgagcagcc
     3181 tcagagtgca tcgcgtggtc ggcgtcgtag cgctccagcc gacgcggctc gccagcagcc
     3241 agatacaact cggcagcgtc catccccgcg atgaagtagt cgtcattggc gaactgcatg
     3301 agcaatggcc tcggcgcgat cgagcccacg tgctcgatcg gatcgagcgg tcgcagcgcg
     3361 cgcatgtact cgaggcggtc ttcgtcgatc ttccagaacc gcacgaacca gtcggcccag
     3421 cggttcgccg cggcgatcac aacgcccgat ccccaacgcg gatcgcgcgc catcagcagg
     3481 agcgcataca tcgccccgaa gtcgtgaccc acgatggcca ggcgatccga gtgacgtgcc
     3541 agccactcca cggcgtccga gaggtcggcc agctcagcct cgatggcctg ggcgtcggcc
     3601 gacgatcccg acggatccaa cttccacgga aagctcagct gcggcagcgc gctggctatc
     3661 ccctcgctcg ccagtgactc cgcctcggcg cggaactgcg tcttgtcgtc gccaccctcc
     3721 tgtggcgcga accagtgcag ccacaggacg cctgcccgcg ccggtccaac cggctcgatg
     3781 cgatcccagt cgctcacccg cgtagccgcg cgatgagcag ccgcttccgc ccggcacgaa
     3841 gcacgaagta gcgccccgcg accggctccg gcagcgacgc ctcgatgtcc gtaacgcgga
     3901 cgtcgttgat cgacataccg ccctgcgtga tgagccgccg cgcctccgac ctggatgtgg
     3961 cgaggccgct cactgccgca agatccagcg cgttcgcagc gtcaccggtg ccgaactcga
     4021 agtggtcgat ggccccgaac agggcgtcga ggacctcggg atcgttgatc ggaccgccgg
     4081 cgaacgcggc atcggcgact ctgacctgtc ggtcggcctc tgcattcccg tgtatgcgcg
     4141 cagtcaggtc gtaggcgagg gcgcgctggg cgggtcggcg ctccggtgac tctgactgct
     4201 ggcgctcgat atcggctacc tggtcggccg tcatcaacgt caaccagcgc aggtgctgga
     4261 ccgccaggga gtcatcgtcg ttgagccagt actggtagaa gtcgtacggc gacgtcatcg
     4321 aggggtcgag catgaccgcg cccttctctg acttgcccat cttc
//
LOCUS       DATLCO010000312         3580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9628537, whole genome shotgun sequence.
ACCESSION   DATLCO010000312 DATLCO010000000
VERSION     DATLCO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9628537"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VMZ33_07190"
     CDS             <1..393
                     /locus_tag="VMZ33_07190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HUR17053.1"
                     /translation="IKGIQPAQLPTLNSLSSLSGSQTFTMVGYGAYEVTNQPGGHQYL
                     YDDVRMKSVGTLNSINKSWLRISQNPATGNSGACYGDSGGPNFLGTTNIVAAITITGD
                     AICRSTNVVYRLDTESARAFLGQFVTLP"
     gene            425..802
                     /locus_tag="VMZ33_07195"
     CDS             425..802
                     /locus_tag="VMZ33_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HUR17054.1"
                     /translation="MSTPDETRIDRWLCAVRLVKTRPLATQLCEGGHVRVNGNPAKPS
                     TKVGAGDRVDALIATRERIVEVVRPIEVRVGAEIAVTCYVDHSPAPVVTDLGRGIKVV
                     RGEGRPSKRLRRELERMKRTLGG"
     gene            831..1424
                     /locus_tag="VMZ33_07200"
     CDS             831..1424
                     /locus_tag="VMZ33_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17055.1"
                     /translation="MNERPADAVRSSLERVLAGPTPDDLWELQKALLAVASPGASRAR
                     AVSRAFHSCLRSLDSKSASRTASRWGAVLGTAAVGAISLPELRDRQERGLGELLEGAL
                     PAVLEIGAAMQTATAWEIEARLIYDEFAWFLYEELWDVSLAARPDLSADERRGRIDTV
                     LNPILDPSVTDADRATLVAEVFMSVLSARVLPLLSGD"
     gene            1661..3073
                     /locus_tag="VMZ33_07205"
     CDS             1661..3073
                     /locus_tag="VMZ33_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17056.1"
                     /translation="MRDGGAAFFASDDDAHIAYQAIRGEHSVVGCDDTEYPYYGEASR
                     SVPPIFSKGGEHLVYGVADGAIGRLVIDGRLADLGILAPIAAVYSPDGSRLAFAEQSP
                     TTDGQYKVRVLVDDVPGPWSMGMRNAGGVMQFSPDSRRFAYLRTDGRGKCQWVVDGRE
                     QQLFSESREISLTSIRLRNIGVLEQPVRATFSPDSQRFVYFADVSGKGVAFVEDDSIG
                     QRFLGLQYPVFSPDSRHLAYVAKMFDKNLALIVDDRVVGKWEAIQAGLPVFSADSAHV
                     ALSILRQTGGPFRKRSLVAATIDGVVIRETPARDISEKPAFSPDGMHLAWWTRDEDQA
                     WVLVDGVEQDLGGQPIGEARFTSAGHLVCRVSTGPESETIVVDGSVGPMASVVGQSMT
                     PKRLAERPGAAPDSAVWRVSADGKHTAWMGDFVDGVFPVLDGEKGPRFEDVISVAFEG
                     DIATWYARNGNQMYAVTARV"
     gene            3207..>3580
                     /locus_tag="VMZ33_07210"
     CDS             3207..>3580
                     /locus_tag="VMZ33_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17057.1"
                     /translation="MQRLGSSGQLIAVFLVGLLVGGTGVVVASAVAQRPDLVGCYDSA
                     GVVHLVASNSRCPAGMFGPINWNVQGPAGERGPAGPAGSRGPTGQQGPAGAQGPAGPP
                     GEAGTGTGSGPISLASLADTDC"
BASE COUNT          623 a         1102 c         1221 g          634 t
ORIGIN      
        1 atcaagggca ttcagccggc gcagctaccc actttgaact ccctgtcgag cctctcgggt
       61 agccagacct tcaccatggt cggatacggc gcctacgagg tcacgaacca gccgggtggc
      121 caccagtacc tgtacgacga cgtccggatg aagtccgtcg ggactcttaa ctcgatcaac
      181 aagagctggc tccggatttc gcagaacccg gcgaccggca acagtggcgc ctgttacggc
      241 gactccggcg gcccgaactt cctggggacg acgaacatcg tggcggcgat cacgatcacg
      301 ggcgacgcga tctgccgctc aacaaacgtt gtgtaccgac tcgacactga gtcagcgcgc
      361 gcattcctcg ggcagttcgt cacgctcccc tgagtcagcc ctgggttgag gtgctggggc
      421 gcgcgtgagc acgcctgacg agacgcgaat cgatcgatgg ctgtgcgctg tccgcctcgt
      481 caagacgcgg ccgctggcga cccagctgtg tgagggcggg cacgttcggg tgaacggcaa
      541 tccggcgaag ccgtcgacca aggtcggcgc cggcgatcga gtcgacgcgc tgatcgctac
      601 acgcgaacgc atcgtcgagg tggtgcggcc gattgaggtg cgtgtcgggg ctgagatcgc
      661 cgtgacctgc tacgtcgacc acagcccggc gcccgtcgtc accgacctcg gacggggcat
      721 caaggtcgtg cgtggcgagg gtcggccgag caaacgccta cgccgcgagc tcgaacggat
      781 gaagcggacc ctgggcggct gaccgaggcg gatgtaactt tggcgtgacc gtgaacgagc
      841 gacccgcaga tgccgtgaga tcgtcattgg agcgcgtcct tgccggaccc acgccggatg
      901 acctttggga gctgcagaag gcactgctcg ccgttgctag tcctggagcg tcacgcgctc
      961 gcgccgtgtc acgcgccttc cactcatgcc tccgatctct cgacagcaag tccgcgtcgc
     1021 gcacggccag tcgctggggc gccgttctcg gcaccgccgc ggttggcgcc atcagcctgc
     1081 cggagctgcg cgatcggcag gagcgtgggc tgggagagtt gctcgagggc gcgctgcctg
     1141 cggtccttga gataggcgcc gcgatgcaga cggcgaccgc gtgggagatc gaggcacgac
     1201 tcatctacga cgagttcgcc tggtttctct acgaggagct atgggatgtc tcgctggccg
     1261 cgcggccgga cctgtccgct gacgagcgcc gcggtcgtat cgacaccgtg ctcaacccca
     1321 ttctggaccc aagcgtgaca gacgcagata gggcgactct cgtcgcggag gtgttcatgt
     1381 cagtgctctc cgcccgtgtg ctcccgctgc tatccggcga ttgatcagag gcgccgaatc
     1441 cagtagacag gtggcgtagt gttgccgcgc gaacgtaacc gccgtcacgg agcgccgcat
     1501 cgacttccac actgagccgc tttctgagct tcccccgggt tgcattccgg atgcggtcgc
     1561 gtcggacggc catgccatct gtttctgggt cagggacgat ggtcgcctca gcttcgtctg
     1621 ggacgatcac atcggtgagc cgttcgacga cgtaggcggc atgcgggatg ggggcgcagc
     1681 gttcttcgcc agcgacgacg acgcccacat cgcgtaccag gcgattcgcg gtgaacacag
     1741 cgtggtcggt tgcgacgaca ccgaataccc gtactacggg gaggcatccc gctcagtccc
     1801 gccgatcttc agtaagggcg gcgagcacct cgtctacggc gtcgcagacg gcgcaatcgg
     1861 acgactggta atcgatggcc ggctggctga cctcggcatc ctggctccga tcgcggccgt
     1921 ctacagcccg gatggttcgc gcctggcatt cgccgagcag tcaccaacga cggacggcca
     1981 gtacaaggtc cgagttctag tcgatgacgt gcccggtcca tggtccatgg gcatgcggaa
     2041 tgccggtggc gtgatgcaat tcagtccgga cagtcggcgg tttgcctacc tgcgcacgga
     2101 cggcaggggc aaatgtcaat gggtcgtcga tgggcgagag cagcagctgt ttagtgagag
     2161 tcgagagatt tctctcacgt ccatacgctt gcgcaacatt ggcgtgctgg agcaacccgt
     2221 gcgggcaaca ttcagcccgg acagccagag atttgtctac ttcgccgacg tgtcgggcaa
     2281 gggggtcgcc ttcgttgagg atgactccat tggtcagcgt ttcctcgggc ttcaataccc
     2341 tgtcttcagc ccagattcga ggcacctcgc ctatgtcgcc aagatgttcg acaagaacct
     2401 ggccttgatc gtcgatgaca gggtcgtggg caagtgggaa gcgatacaag caggccttcc
     2461 agtgttcagc gctgacagcg cacacgtggc gctctcgatc cttcggcaaa ctggtgggcc
     2521 gtttcgtaag cgatccttgg tcgcggccac gattgacggc gtggtgatcc gtgagacgcc
     2581 ggcgagggac atctcagaaa agccagcgtt cagcccagac gggatgcacc tggcctggtg
     2641 gacacgcgac gaagaccaag cgtgggtgct cgtggacggc gtcgagcaag atctgggcgg
     2701 acagccgatc ggcgaggcgc gcttcacttc ggcgggccat ctcgtctgca gagtaagcac
     2761 cgggcccgag tcggagacga tcgtcgttga cgggtctgtg ggacccatgg catcagtggt
     2821 cgggcaatca atgaccccga aaaggctcgc agaacgaccg ggggcagctc ccgacagcgc
     2881 cgtgtggagg gtttcggcag acggcaagca caccgcttgg atgggagatt tcgtggacgg
     2941 cgtcttcccg gtcctggacg gagagaaggg gccgaggttc gaggacgtca tttccgtcgc
     3001 tttcgaaggc gacatcgcga cctggtacgc ccgcaacggc aatcagatgt atgcagtgac
     3061 cgcccgagtc tgaggggctg gtggcggtcg gggggcagaa atgcgaaacc cggcttgtca
     3121 cgggcagggg cgacgacgga gcgctctccg ctacaaggtt ccgtcacgaa tcgaggcctc
     3181 gacgaattag ggcttaacgt tggcggatgc agcgtctggg gtcgagcggg cagctgatcg
     3241 ccgtcttcct ggtcggattg ttggttggcg ggaccggcgt ggttgtcgcg tccgcggtcg
     3301 cgcaacgccc tgacctcgtc ggttgttatg actccgcggg cgtcgtccac ctcgtcgcgt
     3361 ccaactcccg ttgcccggcc ggcatgtttg gaccgattaa ctggaacgtt cagggaccag
     3421 ccggcgagcg cggacccgcg ggccctgccg ggtcacgggg accgacgggt cagcagggac
     3481 ctgccggcgc ccagggacca gcagggcccc ccggcgaggc cggaaccgga acgggatcag
     3541 ggcctatctc gctggcgtcg ctcgcggaca ctgactgcga
//
LOCUS       DATLCO010000313         3994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9628831, whole genome shotgun sequence.
ACCESSION   DATLCO010000313 DATLCO010000000
VERSION     DATLCO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9628831"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VMZ33_07215"
     CDS             <1..537
                     /locus_tag="VMZ33_07215"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HUR17058.1"
                     /translation="IAAIEAFMEGYQVMAGLEAAAWGEVFITATGNLHVFRREHFERM
                     TNGAIMANSGHFDAELDLIALSEMAGGRVRQLRRDVKEYDMGDRRLYVIADGRLVNLA
                     AAEGHPAAVMDMSFANQALAAEYGAKNHASLAPAVHIMPAEIDREVARLKLEALDVQI
                     DEMTPEQKRYVASWQEGT"
     gene            587..1813
                     /gene="metK"
                     /locus_tag="VMZ33_07220"
     CDS             587..1813
                     /gene="metK"
                     /locus_tag="VMZ33_07220"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HUR17059.1"
                     /translation="MTSIVEDQGYLFTSESVTEGHPDKMCDQISDAILDAILREDPTA
                     RVACETATTTGTVIVLGEITTDTYVDFQAVIRDTVRSIGYTDAAYGFDYRTCGTLVSV
                     HEQSADIKRGVDEAIETRGKADQHDQGAGDQGMMFGFACRETPELMPLPITLAHRIAR
                     RLAEVRKEGLVDYLRPDGKSQVTVEYNRGVPVAVRTVVIAAQHDDDVANDRLRDDMIE
                     MVVKEVIPADLRETMPKIHVNPTGRFVIGGPMGDAGLTGRKIIVDSYGGMARHGGGAF
                     SGKDPSKVDRSGAYAARWVAKNVVAAGLADRFELEISYAIGVARPISISIETYGTGKI
                     SDERILGLIDAHFDLRPAAIIDVLGLRKPIYRQTASYGHFGRPDLDLPWERTDLAERL
                     ASEAGLRMPEPVAATA"
     gene            complement(1857..2333)
                     /locus_tag="VMZ33_07225"
     CDS             complement(1857..2333)
                     /locus_tag="VMZ33_07225"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HUR17060.1"
                     /translation="MSSPRDLFPADLLDATRGPTASQAAGLTLAAARAIVAAVQASAR
                     SQGVAMSCAVVDSGDQLVAFERMDGADLVTVTLALDKAYTALANRMPTGELAPIVQPG
                     TEFYGYDSVAGGRMIVFAGGLPLEVDGVLVGALGVSGGSSDQDGAIAEAGRRALTG"
     gene            complement(2330..3103)
                     /locus_tag="VMZ33_07230"
     CDS             complement(2330..3103)
                     /locus_tag="VMZ33_07230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR17061.1"
                     /translation="MTLRRRGLPLYYVLILGLLYLPIAVLFLFSINANTTVAFPLKGF
                     TLDWYGEALTDGALIDAAMNSAQVGVASSTLATILGTITALLLLRFEFRGKRVLAALA
                     VLPLIVPYVVLGVALLILFRAFDISTSLLTVSIAHVVVSMPFVVLIVLSRLIGFDRAL
                     EDAAMDLGATYPQTLRLVVLPIILPAVVSAWLVAFTVSFDEIALALFLTGRDPTFPVY
                     LAGQIRFTATLPVLIAAAVMLMAGSLLLVLTSERIRRAR"
     gene            complement(3100..>3994)
                     /locus_tag="VMZ33_07235"
     CDS             complement(3100..>3994)
                     /locus_tag="VMZ33_07235"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR17062.1"
                     /translation="NNATHPRVARERDPRSGTFKLLLLVPVLFLALFLVAPIVFMAAL
                     SLREDLSGQLLAPFAPTLEHYQQLVDTPSFIRLLLTSAGIAAAVALTTTTLAYPLAYY
                     LRFRAGSRAALLLFLLLLPFWTSYLLRVVAWRVMLGSEGVLNSFLLSTGFVYEPLEGL
                     IYNRGAVIVTLVYVWIPFAAIPILAALQRVKPELHEAAGDLYARPFDQFLRVTLPLSM
                     PGALAAFFMVFIPTVGEYIAPLLVGGTGGTMYGNLIYDFFTRAANWPFGSALSIVMLT
                     ATLALTAVAIRVIDVRRFMPR"
BASE COUNT          797 a         1264 c         1358 g          575 t
ORIGIN      
        1 atcgcagcga tcgaggcctt catggagggc taccaggtca tggcgggcct ggaggcagcg
       61 gcgtggggtg aggtcttcat cacggcaacc ggcaacctgc acgtgttccg gcgcgagcac
      121 ttcgagcgca tgaccaacgg cgcgatcatg gccaacagcg gccacttcga cgccgagctc
      181 gacctgatcg cgctgagtga gatggctggc gggcgagtgc ggcagctgcg ccgtgacgtc
      241 aaggaatacg acatgggcga ccggcgcctc tacgtcatcg ccgacggccg cctggtcaac
      301 ctcgctgccg ctgagggaca cccggccgcg gtcatggaca tgagcttcgc gaaccaggct
      361 ctggcggccg agtacggcgc caagaaccac gccagcctcg cgccagctgt ccacatcatg
      421 ccggcggaga tcgatcgcga agtcgcccgg ctcaagctcg aggcgctgga cgtccagatc
      481 gacgagatga ctcccgagca gaagcgctac gtggcttcct ggcaggaggg aacttgatca
      541 cgatcacttc gcgcgtagct ggctcccggc cagcgaagga gacgtcatga cgagcatcgt
      601 cgaagaccag ggctacctgt tcacatccga gtctgtgacc gagggtcatc cggacaagat
      661 gtgcgaccag atcagcgacg cgattcttga cgcgatcctg cgcgaagatc ccaccgcccg
      721 cgtggcgtgt gaaaccgcga cgacgacagg cacggtcatt gtcctcggcg agatcacgac
      781 cgacacctac gtcgacttcc aggccgttat ccgcgacacc gttcgttcga tcggctacac
      841 cgatgccgcg tatggcttcg actaccgaac gtgcggcacg ctggtgtcgg ttcacgagca
      901 gtcggccgat atcaagcgcg gtgtcgacga ggccatcgaa acccgcggca aggccgatca
      961 gcacgatcag ggtgccggcg accagggaat gatgttcggc ttcgcctgcc gtgagacgcc
     1021 agagctcatg cccctgccga tcacgctggc tcaccgtatc gcgcgccggc tggcagaggt
     1081 gcgcaaggaa ggccttgtgg attacctccg acctgacggc aagtcgcagg tcacggttga
     1141 gtacaaccgc ggcgtgccgg tggccgtgcg gacggttgtc attgctgcac agcacgatga
     1201 cgatgttgcg aacgacaggc tgcgcgatga catgatcgag atggtcgtga aggaagtcat
     1261 cccggccgat ctgcgcgaga cgatgcccaa gatccacgtg aatcccacgg gacgcttcgt
     1321 catcggcggg ccgatgggcg acgcggggct gaccggtcgc aagatcatcg tcgactcgta
     1381 cggcggcatg gcgcgccacg gcggtggcgc cttctccgga aaggatccct ccaaggtcga
     1441 ccgctcaggt gcctacgcgg ctcgctgggt cgcgaagaac gttgtcgcgg cagggctggc
     1501 tgatcgcttc gagctggaga tcagctatgc gatcggcgtg gcgcgaccca tctcgatctc
     1561 gatcgagact tacggcaccg gcaagatcag tgacgagcgc attctggggc tcatcgacgc
     1621 ccacttcgac ctgcgaccgg cggcgataat cgacgttctg ggattgcgca agcccatcta
     1681 ccgtcagaca gcttcgtacg gccacttcgg ccggcccgat ctcgatctgc cctgggagcg
     1741 caccgatctc gccgagcgcc tcgccagcga agccgggctg cgcatgcccg aaccggtcgc
     1801 cgcgaccgcc taggagcgcg ctcgcgccga cccgcgggca gtctggaggc cgtggcctag
     1861 ccggtcagcg cgcggcggcc tgcctcagca atcgctccat cctgatcgga gctgccgccg
     1921 ctgacgccga gagcgccgac gagaacgcca tcgacctcca gtggcaggcc gccggcgaag
     1981 acgatcatgc gtccgcccgc gactgagtcg tagccgtaga actcggttcc gggctggacg
     2041 atcggcgcca gctcaccggt cggcatccgg ttggccagcg ccgtgtaggc cttgtcgagc
     2101 gccagcgtga ccgtcaccag gtcggcgccg tccatgcgct cgaacgcgac cagctgatcg
     2161 cccgaatcaa cgacggcaca ggacatggcc acgccctgcg aacgcgccga agcctggacg
     2221 gcggcaacga ttgcccgcgc agcggccagg gtaagtccgg ccgcttgcga cgcggtcggg
     2281 ccgcgagtcg cgtccaggag atcggccggg aagaggtcgc gcggcgagct caacgtgctc
     2341 gccttatgcg ttcggaggtc agcaccagca ggaggctgcc ggccatcagc attacggcgg
     2401 cggcgattag caccggcagt gtggccgtaa agcggatctg gccggccaga tagacgggga
     2461 atgtcgggtc gcggcccgtc aggaacagtg ccagcgcgat ctcgtcgaag gacaccgtga
     2521 acgccacgag ccaggccgag accacggcgg gcaggatgat cggcagcacc acgagtcgca
     2581 gcgtctgcgg gtaggtggcg cccagatcca tggccgcatc ctcaagggcg cggtcgaaac
     2641 cgatcaaccg cgacaacacg atgagcacga cgaagggcat cgacacaacg acatgcgcga
     2701 tggacaccgt gagcagcgaa gtgctgatgt caaaggctcg gaacaggatc aacagagcca
     2761 cgccaaggac gacgtacggg acgatcaacg gcagcaccgc gagtgcggcg agcacgcgct
     2821 tgccacgaaa ctcgaagcga agcagcaaga gcgcggtgat ggtgcccagg atcgtcgcca
     2881 aggtgctgct cgctactccc acctgcgcac tgttcatggc ggcgtcgatc agcgcgccat
     2941 cggtcagcgc ctcgccgtac cagtcgaggg tgaagccctt gagcggaaac gccacggtcg
     3001 tattggcgtt gatggagaag aggaacagga cggcgatcgg taggtacagc aggcccagga
     3061 tcagcacgta gtagagcggc agcccgcgcc ggcgcagcgt catcgcggca tgaaccgtcg
     3121 aacgtcgatc acgcggatgg cgacggcggt cagggccagg gtcgcggtga gcatcacgat
     3181 tgacagcgcg gaaccgaagg gccagttggc cgcccgcgtg aagaagtcgt agatcaggtt
     3241 gccgtacatc gtcccgccgg tgccgccgac gagcaatggt gcgatgtact cacccaccgt
     3301 cgggatgaag accatgaaga aggccgccag cgccccgggc atgctcaagg ggagcgtaac
     3361 tcgtaaaaac tggtcgaatg gacgcgcata caggtcgccc gcggcttcgt gaagctctgg
     3421 cttgacacgc tgcagcgcag ccaggattgg aatggccgca aacgggatcc acacgtaaac
     3481 gagcgtgacg atcacggcgc cgcggttata gatcagaccc tcgagcggtt cgtagacgaa
     3541 ccccgtcgag agcaggaagg agttgagcac tccttccgag ccgagcatca cccgccacgc
     3601 cacgacgcgc aggaggtagc tcgtccagaa gggcagcaac agcagaaaga gcagcagcgc
     3661 cgctcgagaa cctgcccgga agcggaggta gtacgccagc gggtaggcca gggtcgtggt
     3721 cgtcagcgcg accgctgcgg cgatgcctgc ggaggtcagc aacagcctga tgaacgacgg
     3781 cgtgtcgacc agctgctggt agtgctcgag ggtcggtgcg aatggcgcga gcagctgccc
     3841 cgaaaggtcc tcgcgcaggc tgagagcggc catgaagacg atcggcgcga cgaggaacag
     3901 ggccagaaac aacaccggca cgagcagtaa gagcttgaac gtgccgctgc gcgggtcgcg
     3961 ctcacgcgcg acccgcggat gggtggcgtt gttg
//
LOCUS       DATLCO010000314         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2926345, whole genome shotgun sequence.
ACCESSION   DATLCO010000314 DATLCO010000000
VERSION     DATLCO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2926345"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..306)
                     /gene="secG"
                     /locus_tag="VMZ33_07240"
     CDS             complement(70..306)
                     /gene="secG"
                     /locus_tag="VMZ33_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HUR17063.1"
                     /translation="MQNLLLLGQMLVSIALMASILLQARGAGLGATFGGDSSVYRSRR
                     GIEKRLFQFTVVLAVLFVIFSMAAFRLSTTTPAA"
     gene            complement(367..1911)
                     /gene="gpmI"
                     /locus_tag="VMZ33_07245"
     CDS             complement(367..1911)
                     /gene="gpmI"
                     /locus_tag="VMZ33_07245"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HUR17064.1"
                     /translation="MTPPPRARPLVLVVIDGFGLGSRPADDAIAQANMPHWRRLVAEW
                     PHSRLEASAEAVGLPHGQMGNSEVGHLNLGAGRPVLQDLPRIDAAIADGTFDDTPALL
                     RAARRAAETGARLHLIGLVGPGGVHSVDRHAVEIARLAHSLGVRDVVVHALLDGRDTP
                     PRSADGFVPDLERRLSEAHPGARIATIGGRYFAMDRDQRWERTQRGYEAIVHGRGPSS
                     PSAIRAVLDGYARGESDEFIQPTVIDAVDGRVRADDVVIHFNFRADRARQLTHALVDG
                     DEFDRAAFDRGERPRNLHVVTLTDYEAGLPVHVAFGQMTVSSLSAIFANLGWRQLHIA
                     ETEKYAHITYFFNGGVEAPFDAEQRKLVPSPKVATYDQQPQMSAAGVTDALVEAIRSD
                     HYEFVVVNYANPDMVGHTGVWDATVRAVEVVDECLGRIVDVMPSHGLLCITADHGNAD
                     EMRDAAGNAQTSHSLNPVPVVLIGDAVRGRHMRAGVLADVAPTLLDLLGVPAAEGMTG
                     RSLLDG"
     gene            complement(1908..>2079)
                     /locus_tag="VMZ33_07250"
     CDS             complement(1908..>2079)
                     /locus_tag="VMZ33_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HUR17065.1"
                     /translation="RVPVLYGGSVTSGSIDEFLAEPDIDGALVGGASLKVDEMAGIVA
                     RAGLTAAARKAG"
BASE COUNT          358 a          699 c          708 g          314 t
ORIGIN      
        1 gcctattccg ccacctcggc gcgcagcctt cagcggcgtt tccgacgctt caggcgaacc
       61 ctgatcctat caggccgcgg gtgtggtagt gctcagacgg aaggccgcca tcgagaagat
      121 cacgaataga acagccagca ctacggtgaa ctggaacagt cgcttctcga tgccacgccg
      181 gctccggtac acggacgagt cgccaccgaa tgtggcgccg agaccggctc cgcgcgcctg
      241 gagcaggatc gaagccatca gcgcgatgct cacgagcatc tggccgagga ggagaaggtt
      301 ttgcacgggc ccgagtgtac cgcaaggtcg accggcagcc accggccgag cgcggaggga
      361 gggccttcag ccgtcgagga ggctgcggcc cgtcatgcct tcggcagcag ggacgccgag
      421 gagatcgagc agcgtgggcg ccacatcggc aaggactcca gcgcgcatgt ggcggccgcg
      481 aacagcgtct ccgatgagga cgacggggac agggttgagc gaatgggacg tctgcgcgtt
      541 cccggcagcg tcgcgcatct cgtccgcgtt gccgtgatcg gcggtgatac acagcagacc
      601 gtgactgggc atgacgtcca cgatgcggcc caggcactcg tcaacgacct cgacagcacg
      661 cacggtggcg tcccaaacgc cggtgtgccc gaccatgtcc gggttggcgt aattgacgac
      721 tacgaactcg tagtggtcgg acctgatcgc ctcgacaagg gcgtccgtca ccccggcggc
      781 gctcatctgg ggctgttggt cgtaggtcgc gaccttgggg ctcggcacca gcttccgctg
      841 ctcagcatcg aagggcgctt cgaccccgcc gttgaagaaa taggtgatgt gagcgtactt
      901 ctccgtttcg gcgatgtgta actgtcgcca cccgagattc gcaaagatcg cggacaggct
      961 gctcaccgtc atctgcccga aggcgacgtg gaccggcaaa ccggcctcgt aatcggtcaa
     1021 ggtcacgacg tgtaggttgc ggggccgttc accgcggtcg aacgccgctc gatcgaactc
     1081 gtcgccatcg accagcgcat gcgtcagctg gcgcgcccgg tcggcccgga agttgaagtg
     1141 gatgaccacg tcgtcggcgc ggacgcggcc gtcgaccgcg tcgatgaccg tcggctggat
     1201 gaactcatcg ctctcgccac gcgcgtagcc gtcgagaacg gcccggatcg cggacggcga
     1261 cgaggggccg cgaccgtgga cgatcgcctc gtacccgcgc tgcgtccgct cccaccgctg
     1321 atcgcggtcc atggcgaagt agcggccgcc gatcgtggcg atgcgcgcgc ccggatgtgc
     1381 ttccgagagc cgccgctcga ggtccggcac gaacccgtcc gccgagcgtg ggggtgtgtc
     1441 acgcccgtcg aggagggcat gcaccacgac gtcccttacc cccaaactgt gcgcaagccg
     1501 cgcgatctcc accgcgtggc gatccacaga gtggacgccc ccaggaccaa ccaggccgat
     1561 gaggtgcaga cgagcacccg tctcggcagc cctgcgagcg gcgcggagga gggccggagt
     1621 gtcatcgaac gtcccgtcgg cgatcgccgc gtcaatccgc ggcaggtcct gaaggaccgg
     1681 ccgtccggcg ccgagattga ggtgcccgac ctcactgttc cccatttggc catggggcag
     1741 gccgacagcc tctgccgacg cctcgagcct cgagtgaggc cactcagcga ccaggcgtcg
     1801 ccaatggggc atgttcgcct gtgcgatcgc gtcatcggcc ggtcgcgagc cgagaccgaa
     1861 cccgtcgata acgacgagaa cgagtggtcg tgctcgtgga ggcggggtca ccctgctttg
     1921 cgcgcagcgg ccgtcagccc ggcccgcgcc acgatgccgg ccatctcgtc gaccttgagg
     1981 ctggcgccgc cgaccagcgc gccgtcgatg tccggctcgg cgaggaactc gtcgatcgag
     2041 ccgctcgtga ctgatccgcc ataaagcacc gggacgcga
//
LOCUS       DATLCO010000315         2949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2926968, whole genome shotgun sequence.
ACCESSION   DATLCO010000315 DATLCO010000000
VERSION     DATLCO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2926968"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VMZ33_07255"
     CDS             complement(<1..637)
                     /locus_tag="VMZ33_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUR17066.1"
                     /translation="MTDTLVPQTDSRLPSVLDPAAPGVGSEAPDAFVERLLETQAPQR
                     RMSTPSFTVEVDGKQYTGSEGQTILDICRDNGIDVPTLCYEPKLPGFGACRMCVVEVE
                     GEDQPPISCSRTAEPEMVVRTQTTRLREIRKTNLELIFSDHNAYCLPPCQNKCPSHID
                     IPGFLRANAEANWRESTRIFKRTIPFPSVLGRVCPAPCEDHCRRDEVDEAIA"
     gene            complement(663..1985)
                     /locus_tag="VMZ33_07260"
     CDS             complement(663..1985)
                     /locus_tag="VMZ33_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HUR17067.1"
                     /translation="MNILKGPKGVASVLLARIDKLSTDSPLPAAESAGAWSAWRSTVP
                     QTSPQALVRQMTDSGLRGRGGAGYLTGDKWRAAASVDADVRYVVANGFEADPGAQVDR
                     LLMERDPHAIVEGLALAAYAVGATRAYIAVRANMATALRAFTTAVRAAEEAGYLGTNV
                     LGTGYDLHVEVVAIPGGMVVGEETTLIKAIENKRAQPDQRPPFPTGRGLFGRPTVVNN
                     VETLALVPWIVTNGAAAFAAIGDDTNPGTTLIQISGAVANPGVVEAPLGMSLRKILDL
                     AGGAANAKALLVGGPAGGFLPATELDTLYSPTELAAKGAIWGSGSITVVDESTCIVDM
                     ATLLLRYLSDESCGKTIPCRIGVRRLYELGQRATSGLSRPNDPAVAQQLAADVRDGAL
                     CGLERLAVNPFTSGMRYFAAEFDQHFTRGTCPAGVCSPVRVAAQPVNI"
     gene            complement(1998..2831)
                     /locus_tag="VMZ33_07265"
     CDS             complement(1998..2831)
                     /locus_tag="VMZ33_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HUR17068.1"
                     /translation="MSRMPGWGMAMGMVHTMVRFFQPKVTVQYPDVVQEISVHHRGRL
                     ILLYDEAGNLKCETCFQCAMACPVEIIDMGGVDTKNRYSLHWGPAEQYGERREESPMR
                     RSGRTVPDRTFAAWLPIDLGPLDRILVKHEYDPRRLLSILEDTQAQYGYLPVAAIQHI
                     SHATGAWYSEIYGIATSYANLIFEPGRATEIGICRCPACTMRGGGRIRDALVGALGTD
                     VGGVTVDGSVRFVAAECGGEASAQPLVTVDSQPQPGITTDKARSLAESLRAKSTRPAQ
                     A"
BASE COUNT          480 a          964 c         1003 g          502 t
ORIGIN      
        1 tggcgatcgc ctcatcgacc tcgtcgcgcc ggcagtggtc ctcgcacggc gccgggcaga
       61 cgcggccaag cacgctgggg aacgggatgg tccgcttgaa gatccgcgtg ctctcgcgcc
      121 agttggcctc ggcgttggcc cggaggaacc ccgggatgtc gatgtggctg gggcacttgt
      181 tctggcacgg cggcaggcag taggcgttgt ggtccgagaa gatgagctcg aggttggtct
      241 tgcggatctc gcgtagccgc gtggtctgcg tacgcacgac catctctggc tcagcggtgc
      301 gtgagcacga gatgggcggc tggtcttcgc cctcaacctc gaccacgcac atgcggcagg
      361 caccgaagcc aggcagcttg ggctcgtagc acagcgtggg cacgtcgatg ccgttgtcgc
      421 ggcagatgtc gaggatggtc tggccttcgc tgcccgtgta ctgcttgccg tcgacttcga
      481 ccgtgaagct cggcgtgctc atacgacgct gtggcgcctg tgtctcgaga aggcgctcca
      541 cgaatgcgtc gggcgcctca gatccgacgc cgggcgcggc tggatcgagc accgaaggca
      601 ggcggctgtc tgtctggggc acgagggtgt cggtcatgcc agaaccatca aagccaaaac
      661 catcaaatgt tcacgggctg ggctgcgaca cggaccgggc tgcaaacgcc cgcaggacac
      721 gtgccgcgcg tgaagtgctg atcgaactca gcggcgaagt atcgcattcc gctcgtgaag
      781 ggattgaccg cgagccgctc gaggccgcac agggcgccgt cgcggacgtc ggcagcgagc
      841 tgctgggcga ccgccgggtc gttgggcctg gacaggccgc tcgtggcgcg ctggcccagc
      901 tcgtacaggc gacggacgcc gatgcggcac ggaatggtct tgccgcacga ctcgtcggag
      961 aggtagcgca gcagcagagt ggccatgtcg acgatgcacg tcgattcgtc cacgaccgtg
     1021 atgctgccag aaccccagat ggcgcccttg gccgcaagct ccgtcggtga gtagagcgtg
     1081 tcgagctctg ttgctggcag aaacccgcca gccgggccgc cgacgagcag tgccttggcg
     1141 ttggcggcgc cgccggcaag gtcgaggatc ttgcgcaggc tcatccccag gggggcttcg
     1201 acgacacccg ggttcgcaac cgccccgctg atctggatga gcgtcgtgcc cgggttggtg
     1261 tcgtcaccga tcgcggcaaa ggccgccgcg ccgttggtga cgatccacgg cacgagggcg
     1321 agcgtctcga cgttgttgac aaccgttggc cgcccaaaga ggccgcggcc cgtggggaaa
     1381 ggcggccgct gatctggctg cgcacgtttg ttctcaatcg ccttgatgag cgtcgtctct
     1441 tcgccgacga ccatcccacc agggatggcc acgacctcga catgaaggtc gtaaccggtt
     1501 cccagcacgt tcgtgccgag gtagccggct tcctcggccg cgcgcacggc agtggtgaac
     1561 gcgcgcaaag ccgtggccat gtttgctcgg acggcgatgt aggcgcgcgt cgcgccgacg
     1621 gcgtaggccg ccagagccag gccctcgact atggcgtgcg ggtcgcgctc catgagcagg
     1681 cgatcgacct gtgcgccggg gtccgcctcg aagccattgg cgaccacgta acgcacatct
     1741 gcgtcgacac tggcagccgc gcgccatttg tcaccggtga ggtagccggc gccgccacgc
     1801 ccgcgcaggc ccgagtcggt catctgtcga acgagcgcct gggggctcgt ctgcgggacg
     1861 gtgcttctcc acgccgacca ggcacccgcc gactcggcag ctggaagtgg cgaatcggta
     1921 cttagcttgt cgatgcgtgc cagcagcacg ctggcgacgc ccttcgggcc cttgaggatg
     1981 ttcatctcgg ctgcgagcta cgcctgcgcc ggacgagtgg acttggcgcg gagcgactca
     2041 gccagggaac gtgccttgtc ggtggtgatg cccggctggg gctgactgtc aactgtgacg
     2101 aggggctgcg cggacgcctc gccgccgcac tccgccgcca cgaagcgcac cgagccatcg
     2161 acggtgacgc cgcccacgtc cgtccccaac gcaccaacga gcgcatctcg gatccgccca
     2221 ccgccgcgca tcgtgcatgc cggacagcgg cagatgccga tctccgtggc tcggccgggc
     2281 tcaaagatga ggttcgcgta tgacgtcgcg atgccgtaga tctcgctgta ccacgccccg
     2341 gtggcgtggc tgatgtgctg gatggcagcc acgggcaagt acccgtactg ggcctgggtg
     2401 tcctcgagaa tcgacagcag gcgccgcggg tcgtactcgt gcttgaccag gattcgatcg
     2461 agcggaccaa ggtcaattgg cagccacgcg gcaaacgtcc gatctggcac ggtacggccg
     2521 ctacggcgca ttggcgactc ctcgcgccgc tcgccgtact gctcggctgg gccccaatgc
     2581 aggctgtagc ggttcttggt gtcgacgccg cccatatcga tgatctccac cggacacgcc
     2641 atcgcgcact ggaagcacgt ctcgcacttg aggttgcccg cttcgtcgta gagcaggatc
     2701 agccgtccgc ggtggtgaac ggagatctcc tggacgacgt ccgggtactg gaccgtgacc
     2761 ttcggttgga agaaccgaac catggtgtgg accatgccca tggccattcc ccagcccggc
     2821 attctcgaca tcaaacccat gcttgcgcgc tcaccttctc tggagctcct cgacgagaag
     2881 gattgccccg gccgtcacga agatgttcag gatcgcgatc tcaatcatcc acttccacgc
     2941 gaagcccat
//
LOCUS       DATLCO010000316         4081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5163886, whole genome shotgun sequence.
ACCESSION   DATLCO010000316 DATLCO010000000
VERSION     DATLCO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5163886"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VMZ33_07270"
     CDS             complement(<1..720)
                     /locus_tag="VMZ33_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HUR17069.1"
                     /translation="MDERGFQSPAEIRHRIGPDGTFTLSFVSGSIEVEANDTEEVYVK
                     AKWEHGGDRHRELPLSVRRTDGALHIEADQRDGWFDFRHHGSMEFRVSVPRMARVDIN
                     AVSSDIESHGLLGDQTYKTVSGDVALDDGGGRVSATTVSGDLQLLAKKPVEANLSTTS
                     GDIEAEAAMFRPLRIRTVSGDMEVRGAFDVGPQHTVESVSGDLEITAKTGLTVETKKG
                     LDFSKKNKEPQVVGDGGAHLKF"
     gene            complement(721..1233)
                     /locus_tag="VMZ33_07275"
     CDS             complement(721..1233)
                     /locus_tag="VMZ33_07275"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HUR17070.1"
                     /translation="MQREVISTCPVCESQLHVTRLHCNTCGTTIDGEFNVGRFARLGR
                     DQMLLLEAFLRSRGNLRELERELGVSYPTVRGRVEALIRAIGLSDGTPLPHGSEPKTV
                     EPQVDPQTRRAVLERLSRREITAEQAVAALRGEASAPDTETSAAAGQPADTKAPPAAT
                     NEDDNETQEE"
     gene            1332..2348
                     /gene="ftcD"
                     /locus_tag="VMZ33_07280"
     CDS             1332..2348
                     /gene="ftcD"
                     /locus_tag="VMZ33_07280"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HUR17071.1"
                     /translation="MARLRAEMPDLIECVPNFSEGRRSDVVDALTSPIHSTPGVHLLD
                     RTSDRDHNRSVLTFAGASDAVSTAMLAAVEVAIARIDMHAHEGQHPRIGAVDVIPFVP
                     LGASTIEHCVELAKSFGGQIAERFDLPVYLYANAASRPERVKLAEIRRPQYEGLAEAM
                     ATPEFAPDFGPAVPHPTAGAVAVGARPFLIAYNVNLDTDDLDLAKSIAKEIRESSGGL
                     PRVQGLGLYLDDLRCAQVSMNLLDFNVTPIWRVWEAVASRAASAGVTLRESELIGLAP
                     VAALVDVADHIGMDPGAAVEARITDAARWLKIRDFEPSMALELRLAAAQAASDDAARD
                     ALTL"
     gene            2345..3658
                     /gene="hutI"
                     /locus_tag="VMZ33_07285"
     CDS             2345..3658
                     /gene="hutI"
                     /locus_tag="VMZ33_07285"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HUR17072.1"
                     /translation="MNDERPGLLLHGASAVATLAGGMRRGASQDDVALLSADGVAIGS
                     YEGRIVAVGQLNDVEARLSQHGIATSALTRLDARGGLVTPGLIDPHTHLLFGGTRHAE
                     VGLRQRGLGYLEILAAGGGILQTVRMTRAASDDELLDHGRRWLREMMTHGVTTVEAKS
                     GYGLELDAELRLLGLYGKLGDEGPVEILPTFLGAHAVPPEYRDGSAPAADYLREVIEV
                     QLPEVARQGIARFVDVFCEPGVFTVEQARSLLLAARERGMDVRLHADQINAGGGAELA
                     VEVGARSADHLGAITDAGIAALAAAAAAGRSVVATLLPIASFYLGEEHDAPARQLIDA
                     GVPVALGTDFNPGTSPAPNAQLALAFAVHRLKMSASEALAALTINAAAALGIADTHGS
                     LEEGKHADLVVWKINSHELLPYWLGANLASAVVKRGRVVYEATAD"
     gene            complement(3655..>4081)
                     /locus_tag="VMZ33_07290"
     CDS             complement(3655..>4081)
                     /locus_tag="VMZ33_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR17073.1"
                     /translation="DVHEILVTGIRTFRDEQHGAERGYNMCFVYELDNVHIVHLGDVG
                     HLLSEGDLGEIGTVDVVCVPVGGSLSAAKAAELVAQLDANLVVPMPLDPDGYGREELS
                     KFFHEMSVTHVEPVPKLTVSVSSLPTETTVVVLEPRGKS"
BASE COUNT          635 a         1355 c         1368 g          723 t
ORIGIN      
        1 gaacttgagg tgcgcgccgc cgtcaccgac gacctgcggc tccttgttct tcttcgagaa
       61 gtcgaggccc ttcttggttt cgacggtcag gcctgtcttg gcggtgatct ccaggtcgcc
      121 gctcaccgac tcaacggtgt gctgcggccc gacgtcgaat gcaccgcgga cctccatgtc
      181 accgctgacc gttcggatgc ggaggggtcg gaacatggcc gcctcggcct cgatatcgcc
      241 ggaggtggtg ctcagattcg cctcgaccgg cttcttggcc aggagctgca ggtcgcccga
      301 cacggtcgtg gctgacactc ggccgccccc gtcatccagc gcgacgtcgc ccgacacggt
      361 tttgtatgtc tggtcgccca ggagcccgtg cgactcgatg tccgagctca ccgcgttgat
      421 gtcgacgcgc gccatgcgcg ggacgctgac gcggaactcc atcgagccgt ggtggcggaa
      481 gtcgaaccag ccatcgcgct ggtcggcttc tatgtgaagc gcgccatccg tccggcgcac
      541 cgacagcggc agctcgcggt gcctgtcacc accgtgctcc cacttggcct tgacgtagac
      601 ctcttcggtg tcgttggcct cgacctcgat cgaacctgag acaaagctca gcgtgaacgt
      661 gccatcgggg ccgatacggt gccttatctc ggcgggcgat tgaaaccctc gctcgtccat
      721 ctattcctcc tgggtttcgt tgtcgtcttc atttgtggcg gcgggcgggg cctttgtgtc
      781 ggccggttgg cccgcggcag cgctggtctc tgtgtccgga gcggacgctt caccgcgtag
      841 tgcggccacc gcctgctcag cggtgatctc gcgcctgctc aggcgttcca gcacagcgcg
      901 gcgggtctgc gggtccacct gcggctcgac tgtcttgggt tcagatccat gggggagtgg
      961 cgtcccatcc gacagtccga tggcacgtat cagcgcctca acgcggccgc ggacggttgg
     1021 gtacgagaca ccgagctcgc gctccaactc gcgcaggttg ccgcgcgatc gcaggaaggc
     1081 ctctaggagc agcatctggt cgcggccgag gcgggcgaaa cgcccgacgt tgaactcgcc
     1141 gtcgatcgtg gtgccgcatg tgttgcagtg cagccgcgtc acgtgaagct ggctctcgca
     1201 gacgggacaa gtggaaatga cttctctttg catgtgccgg agactaagta ttgagtttca
     1261 ctttgtcaag tttgatattc acattatgta agtctgggtc cgtcacgagg tagcgtcgca
     1321 ccgccaagac ggtggctaga cttcgcgcag agatgcctga cctgatcgag tgcgtaccca
     1381 acttcagtga gggccgacga tccgacgtgg tggacgccct gactagcccc atccactcaa
     1441 ctccgggggt ccaccttctc gatcggacgt ctgatcgcga ccacaaccgc agcgtcctga
     1501 cgttcgccgg cgcatctgat gcggtgagta ccgcgatgct cgccgccgtc gaggtggcga
     1561 tcgcgcgcat agacatgcat gcgcacgagg gacagcatcc gcgcataggg gccgtggacg
     1621 tgatcccgtt cgtaccgctg ggcgcgtcga cgatcgagca ctgcgtcgag ctggcgaagt
     1681 cctttggcgg acagatagcc gagcgttttg acctccctgt ctacctgtac gcgaacgccg
     1741 cttcgcggcc cgagcgtgtg aagcttgccg aaatccggcg accgcagtac gagggcctgg
     1801 cggaggccat ggcgacgccc gaattcgcgc ccgacttcgg accggcggtg ccacatccga
     1861 ccgccggcgc cgtcgcagtg ggcgcccggc cgttcctcat cgcctacaac gtcaatctcg
     1921 acaccgatga cctggacctg gcgaagtcaa tcgccaagga gattcgcgag agcagcggtg
     1981 gcctgccgcg cgtccagggt ctgggcctgt acctggacga cctgcgctgc gcccaggtat
     2041 cgatgaacct gctcgacttc aacgtcacgc cgatctggag ggtgtgggag gccgtcgcga
     2101 gccgggccgc ctcggcaggt gtgacgctgc gggaatccga gttgatcggg ctggcgcccg
     2161 tggccgcgct cgtcgacgtg gccgatcaca tcggcatgga tcctggcgct gcggtcgagg
     2221 cccggatcac cgacgcggct cggtggctca agatccgcga cttcgagccc tcgatggcgt
     2281 tggagctgcg cctcgccgct gcccaggccg cgtccgacga cgcggcgcgt gatgcgctga
     2341 cgttgtgaac gacgagcggc cggggctgct cctccacggc gcctcggccg tggctacgct
     2401 cgctggcggg atgcgccgcg gggccagcca ggatgatgtc gcacttctct cagcagacgg
     2461 agttgcgatc ggctcgtacg aaggcaggat cgtggccgtt gggcagctga atgacgtcga
     2521 agcgaggctg tcccagcacg gcatcgcgac ttcggcgctg acccggctcg acgctcgcgg
     2581 cggactggtc acccccggcc tgatcgaccc ccacacccat ctgctgttcg gcgggacgcg
     2641 ccacgccgag gtcgggcttc gccagcgcgg actcggctat ctcgagatcc tcgccgcggg
     2701 cggcggcatc ctccagacag tgcggatgac ccgcgccgcc agcgatgacg agctcctcga
     2761 ccacggtcgc cgctggctgc gcgagatgat gactcacggc gtcacgacag tcgaggcgaa
     2821 atccggttac ggcctggagc tcgatgccga gctgcggctg ctgggccttt acggcaagct
     2881 gggcgacgag gggccggtcg aaatcctgcc cacgttcctc ggcgcgcacg ccgtgccacc
     2941 cgaatatcgg gacgggtctg cgccggcggc cgactacctg cgcgaggtga ttgaagtcca
     3001 gctgcctgag gtcgcacgac agggaatcgc gcggttcgtc gacgtcttct gcgagccggg
     3061 cgtgttcacc gtggagcagg cacgctcgtt gttgctcgcc gcacgcgagc ggggaatgga
     3121 cgtgcggctc cacgccgacc agatcaacgc aggcggtggc gccgagctgg cggttgaggt
     3181 cggcgcgcgc tctgccgatc acctgggcgc catcacggac gccgggatag ccgcgcttgc
     3241 ggccgccgcg gccgccggcc ggtccgtcgt ggcgacgctg ctgccgatcg cgagcttcta
     3301 tctcggcgag gaacatgatg cgccggcgcg acagctcatc gacgcgggcg tgccggtcgc
     3361 cctggggacc gacttcaacc cgggcacgtc gcccgcccca aacgcgcagc tggcgcttgc
     3421 cttcgccgtc catcgcttga aaatgtcggc gagcgaggcg ctggcggcgc tgaccatcaa
     3481 cgcagcggct gccctcggga tcgcggacac gcacggctcg ctcgaggaag gcaagcacgc
     3541 cgacctggtg gtgtggaaaa tcaattccca cgaactgctg ccctattggc tcggcgcaaa
     3601 cctcgcgtcg gccgtagtga agcgtggccg cgtcgtctac gaggccacgg cggactagct
     3661 cttgccgcgc ggctccagga cgacgaccgt cgtctcagtc gggagcgagc tcacgctcac
     3721 cgtcagcttc ggcacaggct cgacgtgcgt gacgctcatc tcgtggaaga acttgctgag
     3781 ctcctcgcgt ccatagccat cggggtcgag gggcatcggc accacgagat tcgcgtccag
     3841 ctgcgcaacc agctcggcgg ccttcgcggc tgagagtgat ccaccaactg gaacgcacac
     3901 gacgtcgacc gtgccgatct cgcccaggtc gccctcggaa agaaggtgcc cgacgtcgcc
     3961 caggtggacg atgtgcacgt tgtcgagctc gtagacgaag cacatgttgt agccacgttc
     4021 ggcgccgtgt tgctcatctc ggaacgtacg gatcccggtg acaaggatct cgtgaacgtc
     4081 g
//
LOCUS       DATLCO010000317         6420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_9640662, whole genome shotgun sequence.
ACCESSION   DATLCO010000317 DATLCO010000000
VERSION     DATLCO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9640662"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VMZ33_07295"
     CDS             complement(<1..198)
                     /locus_tag="VMZ33_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HUR17074.1"
                     /translation="MTTPKLAQRMVGIGTETAFEAAAKGRALEAQGRHIVHLEIGEPD
                     FDTPANIREAAKKALDEGWTHY"
     gene            complement(230..3337)
                     /locus_tag="VMZ33_07300"
     CDS             complement(230..3337)
                     /locus_tag="VMZ33_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17075.1"
                     /translation="MDDQRLHDELARRAESVRVEPDWSRRHLLSSVRHAIDTRPSRVS
                     TTSRWSASAGLASVAAILLVLVFVAPRLAQGPAASDVPTSSETSRQEPSPLPGPRLAM
                     SPKEFASVLTAGDLAGRTVLVEGRIDWDQQDGISLYGVACVDGDGIQTVNPSFSCLLG
                     HLGDVEAPVWVDAPYVQTGTSEWRAPQPPAVGLLVLEVSDSSVHYLGTANTTGPSWPH
                     KVQELAAIPASAVAFSTVHIVEGWLIETEPNGQNITIDCDGPAFPAIPGLPTRHCQPV
                     DFLADEPAQPDDRGSLGTSDRLTVQRDAAVGFAPDEPTEKAVYAVAPRLYGGGCVGEP
                     PCWLWEVIARVSEPPALAPTTPTPAPATPTPAPLGIIECTAEISVTVTDYIGEVVSCD
                     FERMAPESIAGPTQVANPDGELRRLDISWHGSACATKVDVDFQATTDIDAGDRYRLSI
                     TQFDQPACLGPRTQSRLHLQLTKDVAADLVQAEVSTVPDSPATPTPAPLGVMDCAGGL
                     PDVTLIDYIGEVVSCEASGTGLMFETTAQNVTNPDDDLTVLEIVWPGNPCADSIDLQF
                     AATLEEYRLVITELETSNTICDYAYPDRRTVRLQLTKPVPAENIRVDVQEDWDPPPPP
                     SSGLFVCGPMTASPGELRRWHPGISDPSGLIASCDASAVVGTDSKPIAISNPDGDLRT
                     LEVTWTDACASYETLALLWRGHGAAYDLIAHNSMPANVICDGLVLDHVVRLHMSLDVS
                     AADVVASVSRADPVALPTPEIAPTRFECHGPPAPPGTTPVPGPVPVVVDTTGLVSACL
                     QTDSVEPLTGPIGVSNPFEDHAIEVVWATGVCDNAIEFTFATGTEHAYELVGARPPDC
                     KASVSPLPVYITFSQPMLAADIHAVLENASNVRRTSVGDFRLSIFANRREFEAEDLID
                     ISASLLYQGGSESITVAGVGTLVNGFSVRQLDGELSMGPAWNEPCIPETLTREVPYEV
                     DFEKSGGYGPDDPNRDFYMRYLADPELRLPSGVWLITAHSEFWAGACSGDYVSLTASV
                     VVRVR"
     gene            complement(3337..3873)
                     /locus_tag="VMZ33_07305"
     CDS             complement(3337..3873)
                     /locus_tag="VMZ33_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUR17076.1"
                     /translation="MDRELLERARAGDREAFELIVVAKGEPLFRTALAILGNEADARD
                     ATQEAFVSCWRSLSTVRDVDRFDAWLGRILINECRMTLRQRRRIRELMVQQTSDGEVA
                     ATPAMGALDTAESGDFDAAFGRLSVDQRAILVLHHLHGYGVREIGAWLGIPSGTVKWR
                     LSRARSALRGELAGQGES"
     gene            3973..4371
                     /locus_tag="VMZ33_07310"
     CDS             3973..4371
                     /locus_tag="VMZ33_07310"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HUR17077.1"
                     /translation="MARYFKLDEANDILREAQPVLAQLRADRNRVAAIQATLQRERES
                     NGSVEHAEAMGQQENQLVDIVRRMQEAVAQIDAWGITLRDISTGLIDFPALANGRPIW
                     LCWRLGEGDIEWWHETNVGFDGRQPLAELT"
     gene            complement(4581..6068)
                     /locus_tag="VMZ33_07315"
     CDS             complement(4581..6068)
                     /locus_tag="VMZ33_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17078.1"
                     /translation="MAQYALPPGAIKRRTAFGLLDADGWGWATVKALFWFFLVIFLLG
                     YVPDRAYYFTVSPTIDLGFNVISPINLCPAENKTLPCPAPAGAVIPWEQSPDNLSLPQ
                     GRAAAGVFTSGETIYLIGGRTDAGATTSVLTADVFEGNLTQWAEGPALPEPRSDAAVA
                     SVSGVPYVIGGLDASGAPSTTVFQGVVESGQLTGWEVATAAALPAGVSDSAAVGTGTS
                     LFLFGGRTAEGLSATVWRAHLNDAGTELEAWAPVTELPLPEARADHTALSAAGAVYIV
                     GGEGPSGTASSVFYLALDSHGEPTIDHDSGRVFGWGVSAGSSAAAALPEPRRGQTSFT
                     NSGTIFALGGYDANNALAATNLWTVPSATNGTIRAWSQRDETNLLAPRAEAAAALVGQ
                     HVFVIGGVGEGGALQTSSFRADVAPRPAFFRLGLFGLTVPALSIKGEIGQQLGYIIAG
                     SAALGNFVLLVVIGWMYSHRRETMRFFQWISRGRLRAPREDEYQT"
     gene            complement(6084..>6420)
                     /locus_tag="VMZ33_07320"
     CDS             complement(6084..>6420)
                     /locus_tag="VMZ33_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17079.1"
                     /translation="SGLEHNFHIAGAEELSTAPKENGLPGTPTFKEGTQEVSYTFESD
                     AELQFACTVEGHYPSMHGDFAYVTEGGSASPGASPAGSADPAASVDPLASAEASPVPS
                     VLTSPAPVQ"
BASE COUNT         1132 a         2023 c         2186 g         1079 t
ORIGIN      
        1 gtaatgcgtc cagccctcgt cgagtgcctt cttcgcggcc tcacggatgt tggcgggagt
       61 gtcgaaatcg ggctcgccga tctccaggtg gacgatatgc cggccctgcg cctcgagcgc
      121 gcgacccttc gcggcggcct caaaggctgt ctcagtgccg atgccaacca tgcgctgggc
      181 gagctttgga gtcgtcacgg cgccgatgct agcaggggcg atctacccct cagcgcacgc
      241 gcaccaccac gctcgccgtg agactgacgt agtcaccgct gcacgcgcct gcccagaatt
      301 ccgagtgggc cgtgatcagc cagactccgc ttggaagacg aagttccggg tcggccaggt
      361 agcgcatgta gaagtcgcgg ttgggatcat ccgggccgta gccgcctgac ttttcgaaat
      421 cgacctcgta gggcacttcg cgcgtcagtg tctcgggaat acacggctcg ttccacgccg
      481 ggcccatcga caattcgccg tccagctgtc gtaccgagaa cccgttcacg agggtgccca
      541 cgccggcgac ggtgatcgac tcggacccgc cttggtacag aagcgaagcg ctaatgtcga
      601 tcagatcttc cgcctcgaat tctctccggt tggcaaaaat ggacaagcgg aagtcgccca
      661 ccgaggttcg tcgcacgttg ctggcattct cgagaaccgc gtggatgtcg gctgccagca
      721 ttggctggct gaaggtgatg taaaccggaa gcggagatac cgaagccttg cagtccggag
      781 ggcgggcgcc aacgagctcg taagcgtgct cggtgcccgt ggcgaatgtg aattcgattg
      841 cgttgtcgca gacaccagtc gcccatacaa cctcgatggc gtgatcttcg aaggggttgc
      901 tgacgccgat cgggcccgtc aacggttcga ccgagtccgt ctggagacag gcgctgacca
      961 gtcctgttgt atcaacgaca acaggcacgg ggccgggaac gggcgttgtc cccggcggag
     1021 cgggtgggcc gtggcactcg aatcgcgtcg gggcaatctc gggcgtcggc agcgcgactg
     1081 gatcggctct actgacgctc gcgacgacgt cggccgccga gacgtcgagc gacatgtgta
     1141 ggcgcacgac gtggtcgagc acgagaccgt cacagatgac gttggcgggc atcgaattgt
     1201 gggcgatgag gtcgtaggcg gctccgtggc cacgccagag gagagccagc gtttcgtagc
     1261 tcgcgcacgc gtctgtccac gtcacttcga gcgtgcgcaa atcaccgtcg gggttcgaga
     1321 tcgcgatggg tttcgagtcc gtaccgacaa ctgccgacgc gtcgcaggag gcgatcagcc
     1381 ccgacgggtc agagatgccc gggtgccaac gccgaagctc ccccggcgaa gctgtcatcg
     1441 gtccacaaac aaagaggccc gatgaaggtg gcggtggcgg gtcccaatct tcttggacgt
     1501 cgacgcggat gttctcggcc ggaacgggtt ttgtcagttg aagtcggacg gtcctgcgat
     1561 cgggatacgc gtagtcgcag atggtgttcg aggtttccaa ctcggtgatg actagtcgat
     1621 attcctcgag cgtggccgca aactgaaggt caatgctgtc ggcgcaggga tttcccggcc
     1681 acacgatttc aaggacggtg aggtcgtcat ccgggttggt cacgttctgg gcggtggtct
     1741 cgaacatcag gccggtccct gaggcttcgc agctcacgac ttcgccgatg taatcgatga
     1801 gcgtcacgtc aggaagaccc cccgcgcagt ccatgacgcc gagaggggcc ggagtcgggg
     1861 tcgcggggga gtccggtacg gtgctgacct cggcctgcac cagatctgcg gcgacatcct
     1921 tcgtgagttg gaggtggagt cggctttgag ttcggggacc caggcaggca ggctggtcga
     1981 attgcgtaat tgagaggcgg taccggtcgc ccgcgtcgat atcggtcgta gcctgaaagt
     2041 cgacatcgac cttcgtcgca cacgcgctgc cgtgccaact gatgtccaga cgccgcagtt
     2101 ccccatccgg atttgcgact tgcgttgggc cagcgattga ctcgggtgcc atgcgctcaa
     2161 aatcgcagct cacgacttcg ccgatgtagt cggtgacagt gacagagatc tcggcggtgc
     2221 attcgatgat cccgagaggc gccggagtcg gggtcgccgg cgctggcgtg ggcgtcgttg
     2281 gtgccagcgc cggtggctcc gacacacggg caatcacttc ccacagccaa cacggtggct
     2341 cgccgacgca accgccgccg tacaggcgcg gcgcaactgc gtagacggcc ttctccgtcg
     2401 gctcgtctgg ggcgaatccc acggccgcgt ctcgttgaac agtgagtcga tcgcttgtgc
     2461 cgagcgatcc gcgatcgtcc ggttgcgcag gctcatcggc taggaagtcg acaggctggc
     2521 agtggcgcgt cggcagaccg ggtattgcgg gaaacgcagg gccatcgcaa tcgatggtga
     2581 tgttctgccc attgggttcg gtttcgatga gccagccttc gacgatgtgg acggtgctga
     2641 aggcgacggc gctcgcaggg atcgccgcca attcctgcac cttgtgcggc cacgaaggtc
     2701 ccgtggtgtt ggccgtcccc aagtaatgaa ccgaggagtc cgacacttcc agaaccagca
     2761 agccgaccgc gggcggctgc ggcgcgcgcc actcggacgt tccagtctga acgtatggcg
     2821 cgtccaccca aacgggtgct tcgacgtcgc cgaggtggcc cagcaggcaa gaaaagcttg
     2881 gatttacggt ctggatgccg tcaccgtcca cgcatgcgac gccgtacagg ctgatgccgt
     2941 cctgctggtc ccagtcgata cgcccttcaa ccagcaccgt cctcccggcg aggtcgccgg
     3001 cggtcaggac ggatgcaaat tcctttggcg acatcgcgag ccgcggaccg ggaagcgggg
     3061 aaggctcctg gcggctggtc tcgctgctgg ttggcacgtc ggacgctgct gggccctggg
     3121 cgagccgcgg ggccacgaac accagcacca gcaggatggc tgcaaccgag gctaatccgg
     3181 cggacgcgga ccagcgcgag gtcgtcgaga cgcgcgaagg tcgagtgtcg atcgcgtggc
     3241 gaactgacga gagcagatgt cgacgggacc aatcgggctc gacgcgcacc gactctgcgc
     3301 ggcgcgccaa ctcgtcgtgg agtcgctgat cgtccatcag ctctcgccct gcccggctag
     3361 ctcaccgcgc agcgcgctcc gcgcccgcga gagtcgccac ttcaccgtcc ccgacgggat
     3421 gcccagccac gccccaatct cgcgcacgcc atacccgtgc aggtgatgga gcacgaggat
     3481 cgcccgctgg tccacgctca atcggccaaa ggcggcgtcg aaatcgcccg actccgcggt
     3541 gtcgagggcc cccatggccg gtgtggcggc gacctcacca tcggacgtct gctggaccat
     3601 cagctcacgg atgcgtcgcc gctgacggag cgtcatgcgg cactcgttga tcaggatgcg
     3661 cccgagccac gcatcgaagc gatcgacgtc gcgcacggta ctcaggctgc gccagcaact
     3721 gacgaaggct tcctgggtgg cgtcgcgcgc gtcggcctcg ttccccagga tcgccagcgc
     3781 ggtcctgaaa agcggctcgc cctttgcgac aacgatcaac tcaaacgcct cccgatcccc
     3841 ggcgcgcgcc cgttccagga gctcgcggtc catccactcc cctcgtgcag cgtgccttct
     3901 gaatagatgc ttggaaacgc agatcggttg gcgttctgct ggcgatcgtc aggctagtgc
     3961 gagaatcgcc ccgtggcgcg ctatttcaag ctcgatgagg cgaacgacat cctgcgcgaa
     4021 gcccagcccg tgctcgccca gctgcgggcc gatagaaacc gggtcgccgc gatccaagcg
     4081 actttgcagc gcgagcggga gtcgaacggg tcggtcgagc acgcggaggc catgggccaa
     4141 caggagaacc agctggtcga catcgtgcgg cgcatgcagg aggcggtcgc ccagatcgac
     4201 gcttggggga taaccctgcg cgacatctcg accgggctga tcgactttcc agccttggcc
     4261 aacggtcggc cgatctggct gtgctggcgc cttggcgagg gtgacatcga gtggtggcac
     4321 gagacgaacg tcggcttcga cggccgccag cctctggcgg agctcaccta gcccctgctc
     4381 ttggccagat tcccgtcaca ccacactagg gcgcggacgg tggcgatctt ggctaccacg
     4441 ttgcagcgtg agtgccgcta ggccggatac gaactcggtc tgacgctcag tgggctaaag
     4501 cttgccgaaa ggcagtgcgc ataacggtgg tgtagttcga atctggccaa atgctgggcg
     4561 gggcggggcg ggcgagtgcc ctacgtctgg tattcgtcct cgcggggggc cctcagccgg
     4621 ccacgtgaga tccactggaa gaagcgcatc gtctcgcggc ggtggctgta catccagccg
     4681 atgaccacca ggagcacgaa gttgcccaac gccgctgacc cggcaatgat gtagccgagc
     4741 tgctggccga tctcgccctt gatcgacagg gcgggcaccg tgagcccgaa aagtcccagg
     4801 cggaagaacg cgggccgcgg cgcgacgtcg gcgcggaagc tcgacgtctg gagtgcaccc
     4861 ccctcgccga cgccgccaat aacgaacaca tgctggccaa cgagcgcagc cgctgcctcg
     4921 gcacgcggcg ccagaaggtt ggtttcatcg cgctgactcc aggcgcggat ggtgccgttg
     4981 gtcgcgctgg ggacggtcca caggttcgta gcggcgaggg cgttgttggc gtcgtagcca
     5041 cccagcgcaa agatggtgcc ggagttggtg aacgacgtct gcccgcggcg tggttcaggc
     5101 agcgcggcgg cggcggatga gccagcggag acaccccagc caaagacacg cccgctgtcg
     5161 tggtcgatcg tcggctcgcc gtgcgagtcg agcgccaggt agaacaccga gctcgccgtg
     5221 ccgcttggcc cctcgccgcc gactatgtac acggcgccgg ctgcgctcag tgcagtgtga
     5281 tcggcgcgtg cctcggggag tggcagctcg gtcacgggcg cccaagcctc aagctcggtg
     5341 cccgcgtcgt tgagatgggc acgccaaacg gtggccgaaa ggccctccgc cgtacggcca
     5401 ccaaagagga acaggcttgt gccggtaccg acggcagccg aatcgcttac gccagccggc
     5461 agggccgcag ccgtggcaac ttcccagccg gtgagttggc ccgactcgac cacgccctgg
     5521 aacaccgtcg tcgacggagc cccggaagcg tccagaccgc cgatcacgta cggaacgccg
     5581 ctcacgctcg cgactgccgc gtcggatcgt ggctcgggta gagcgggacc ctcggcccac
     5641 tgggtgaggt tgccctcgaa gacgtcagcg gtgaggacgg aggtcgtagc gcccgcatcc
     5701 gtgcggccgc caatcagata gatggtctcg cccgacgtaa agacgccggc agcggcacgg
     5761 ccctgaggta gagagaggtt gtcggggctt tgctcccacg ggatgacggc accggctggc
     5821 gccgggcacg gcagtgtctt gttctcggcg gggcagaggt tgatcgggct gatgacgttg
     5881 aaacccaggt cgatcgtcgg cgagacggtg aagtaatagg cacggtcggg gacgtagccg
     5941 agcaggaaga taaccaggaa aaaccagaag agcgccttga ccgtcgccca gccccaaccg
     6001 tcggcgtcga gcaggccgaa ggccgtgcgt cgttttatcg cgccgggcgg gagtgcgtac
     6061 tgggccatct agcgacgcct catctactgc acgggggccg gcgaagtcag gaccgacggg
     6121 acaggcgaag cttccgcgga ggcgagcggg tctacggaag cggccggatc tgccgagccc
     6181 gcaggcgacg caccgggtga cgcgctgccg ccctcagtga cgtatgcgaa gtcaccgtgc
     6241 atcgacggat agtgaccttc gactgtgcag gcgaactgga gctcagcgtc actctcaaat
     6301 gtgtaactga cctcctgcgt gccctccttg aatgtgggcg tgcccggcag gccgttctcc
     6361 ttgggtgctg tagacagctc ctcggcgccg gcgatatgga agttgtgctc aaggccagag
//
LOCUS       DATLCO010000318         3349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10794088, whole genome shotgun sequence.
ACCESSION   DATLCO010000318 DATLCO010000000
VERSION     DATLCO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10794088"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /gene="prfA"
                     /locus_tag="VMZ33_07325"
     CDS             <1..1123
                     /gene="prfA"
                     /locus_tag="VMZ33_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HUR17080.1"
                     /translation="GGRPPRAMTLELRLDELEQRLLEIEAEWSNPEVSADHDRSRLLG
                     QEQAQLAPVVGDYRLLRRVREQLEAARRDRAAESDPEVRDMAAALIDELEADEVRLTE
                     ALRVHLLPTDPNDERNVIIEIRAGVGGEEAALFAAELFRMYARYAERRRWKTDLITAS
                     ETGIGGFKEVIFEVHGAGAYSRLKYEAGPHRVQRVPETESGGRLHTSTATVAVLPEAD
                     EVEVQIDDKDLKIDVYRSQGPGGQSVNTTDSAVRITHLPSGLVVAIQDEKSQHKNKAK
                     ALAVLRARLLEMEQRKAHEAEAEVRRSMVGSGERSEKIRTYNYPQDRVTDHRINVSVH
                     NLPNVLDGDLDGLLDQLIAIDQAQRLSELERTPTDGAAD"
     gene            1110..2030
                     /gene="prmC"
                     /locus_tag="VMZ33_07330"
     CDS             1110..2030
                     /gene="prmC"
                     /locus_tag="VMZ33_07330"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HUR17081.1"
                     /translation="MRPTETPATVGSALREAAARLTEAGSETPRLDAEVLLGHVLGFE
                     RATLLAHLDLALPPAQMSTFEGLIARRGTGEPVSYIRGFKEFYGLALAVDRRALIPRP
                     ETELVVNLALATIGRRLTESARPAGTGPYRVWDVGTGSGAIVVALAVESRRHRYDSDV
                     RFRATDASPDALAVAVENAVGHGVADLIEFSVADLAHEAENGFDLVVANLPYVPSADI
                     PTLPVAASFEPVSALNGGPDGLDVIRRLAAQLPTVLVAGGTALLEIGAGQADAVRVFV
                     EERLPGWSSEIHPDLANVPRVVELTRPVAA"
     gene            2027..2653
                     /locus_tag="VMZ33_07335"
     CDS             2027..2653
                     /locus_tag="VMZ33_07335"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HUR17082.1"
                     /translation="MTRRISASDPRAIDEAVAALRRGELIAIPTETVYGLAVLPTTTG
                     LERLIAAKHRSAEKGVQLLIDSIDQATALADVTPAARALAARYWPGPLTIVLNRRTDV
                     EVPETLGGGRPTLGLRLPDHEVPRAIARALGPIAASSANVSGEPDATTADHVAGFFTD
                     EISLIVDDGQVRGGVPSTVVSCADARQPPQVLREGAISTAEIAAVAAD"
     gene            2663..>3349
                     /locus_tag="VMZ33_07340"
     CDS             2663..>3349
                     /locus_tag="VMZ33_07340"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HUR17083.1"
                     /translation="MSVETRSRIAWASLAEVDPDLWTAMQGERERQRWNIELIASENY
                     AFAAVLEAQGSWLTNKYAEGLPGRRYYGGCEFVDEAERLAQQRALDLFPDAEHVNVQP
                     HSGAQANMAAYISVLNMGDRVLGMNLAHGGHLTHGSPVNFSGKWFDIHAYGVREEDAR
                     IDYDALDKQAHEVRPKMIVAGASAYPRIIDFERLGRIARDVDALLFVDMAHIAGLVAA
                     GLHPSPFPHAD"
BASE COUNT          564 a         1118 c         1128 g          539 t
ORIGIN      
        1 cggcggccga ccaccacgcg caatgacact cgagctgcgc ctcgacgagc tcgagcagcg
       61 gctcctggag atcgaggccg agtggtccaa ccctgaggtt tcggccgatc acgaccgctc
      121 ccggctcctt ggccaggagc aggcccagct tgcacccgtt gttggcgact atcggctcct
      181 gcgccgcgtg cgcgagcagc tcgaggcagc gcggcgggac cgcgcggctg agtccgatcc
      241 ggaggtgcgc gacatggccg ccgcgctcat cgatgagctg gaggctgatg aggtacgcct
      301 gaccgaggca ctgcgggtcc acctgctgcc gaccgacccg aacgacgagc gcaacgtcat
      361 catcgagatc cgcgccggcg ttggcgggga ggaggcggca ctcttcgctg ccgagctgtt
      421 caggatgtat gcccgctacg cagagaggcg acgctggaag accgatctga tcacggccag
      481 cgagaccggc atcggcggtt tcaaggaggt catcttcgag gttcacggcg cgggtgccta
      541 cagccgactg aagtacgagg ccgggcctca tcgcgttcag cgcgttccgg agacggaatc
      601 gggcggccga ttgcacacgt cgacggcgac ggttgcggtt ctgcccgagg cggacgaggt
      661 cgaagtccaa atcgacgaca aggacctcaa gatcgacgtt taccgatcgc agggaccagg
      721 cggccagagc gttaacacca ccgactccgc ggtcaggatc acgcacctcc cgtcgggtct
      781 cgtggtcgct atccaggacg agaagagcca acacaagaac aaggccaagg ccctggcggt
      841 gctccgtgct cgtctccttg agatggagca gcgcaaggcg cacgaggctg aggcggaggt
      901 gcggcgttcc atggttggct cgggcgaacg ctctgagaag attcgcacct acaactaccc
      961 gcaggaccgt gtcaccgacc accggataaa cgtgagcgtc cacaacctgc ccaacgtcct
     1021 cgatggcgat ttggacggtc tgctcgacca gctcattgcc atcgatcagg cgcagcgcct
     1081 gtccgagttg gagcgcacgc cgaccgacgg tgcggccgac tgagacgccg gcgacggtcg
     1141 gtagcgcact gcgcgaggcg gctgcgcggc tgaccgaagc gggatcggag actccgcggc
     1201 tcgacgctga ggtgcttctc gggcacgtgc tcggcttcga gcgcgcgacg ctactcgcac
     1261 acctggatct ggccctgcca cctgcccaaa tgagcacgtt tgagggcctt attgcacggc
     1321 gtgggacggg cgaaccagtg tcgtacatcc gcggtttcaa ggagttctac ggactcgcac
     1381 tggccgtgga caggcgggcg ctgatcccgc ggcccgaaac cgagctggtt gtgaacctcg
     1441 ccctggccac catcggtagg cgtctcaccg agagtgctcg cccagccgga accgggccgt
     1501 accgtgtgtg ggacgtgggc acgggcagcg gcgcgatcgt cgtcgccctt gcggtcgagt
     1561 cacgccgcca ccgctacgat tccgacgtgc gattccgcgc caccgatgca tctccggacg
     1621 cactggccgt ggccgtggaa aacgctgtcg gccacggagt cgcggacctc atcgagttct
     1681 ctgtggccga tctcgcgcac gaggcggaga acggctttga ccttgtcgtt gcgaacctgc
     1741 cttacgtgcc atcggccgac attccgacac tccccgtcgc cgccagcttc gagcccgtgt
     1801 cggcgctcaa cggtgggccc gacggcctcg acgttatccg tcgtctggcg gcgcagctgc
     1861 cgacggtgct cgtggccggc ggcacggcgc tgctcgagat cggcgccggt caggcagacg
     1921 cggttcgggt gttcgtcgag gagcggcttc cgggctggtc gagcgagatc caccccgacc
     1981 tcgccaacgt gccacgtgtc gtcgagctga cgcggccggt ggccgcgtga cgcggcgtat
     2041 ctctgcgagc gatccacggg cgatcgacga ggctgtcgcc gcccttcgtc gcggcgagct
     2101 catcgccatc cccactgaga cggtctacgg cctcgcggtt ctgcccacga ccaccggcct
     2161 ggagcggctg atcgccgcga agcaccgctc agccgaaaaa ggcgttcagc tgctgatcga
     2221 ctccatcgac caggcgacgg cgctggccga cgtgacgccc gccgcccgtg ctcttgcggc
     2281 tcggtactgg ccggggccat tgacgatcgt gctcaatcgc cgtaccgacg tcgaggtccc
     2341 ggagaccctc ggcggcgggc gcccaaccct tggcctgcgg ctgcccgacc atgaggtgcc
     2401 gcgcgccatc gctcgcgccc ttggcccgat cgctgcgagc tcggccaacg tgagtggcga
     2461 acccgatgcg acgaccgcgg atcacgtggc cggcttcttc acggacgaga tctcgttgat
     2521 cgtggacgac ggacaggttc gtggcggcgt gccgtcgacc gtcgtatcgt gtgccgacgc
     2581 tcggcaacca ccccaggtcc tccgcgaagg tgcaatctcg acggcggaaa tcgcggccgt
     2641 cgccgccgac taaactgacc tcgtgagtgt cgaaacgagg tcccgcatcg catgggcgag
     2701 ccttgccgag gtagaccccg acctgtggac cgcgatgcag ggcgagcggg agcgccagcg
     2761 ctggaatatc gagctgatcg cgagtgagaa ctacgcgttc gcggcagtcc ttgaggcgca
     2821 gggctcttgg ctgaccaaca agtacgccga gggcctgccc gggcgccgct actacggtgg
     2881 ctgcgagttc gtcgacgagg ctgagcgact ggcgcaacag cgggcactcg acctcttccc
     2941 cgatgctgag cacgtcaacg tgcaaccaca ctccggcgcg caggcgaaca tggccgcgta
     3001 tatctccgtg ctcaacatgg gcgaccgggt actgggcatg aatttggcac atggcggaca
     3061 cctgacccac ggctcgccgg tgaacttctc gggcaaatgg ttcgacatcc atgcctatgg
     3121 ggttcgcgag gaagacgcac ggatcgatta cgacgcgctc gacaagcagg cgcacgaggt
     3181 ccggccgaag atgatcgtgg ccggggccag cgcctatccg cggatcatcg atttcgagcg
     3241 cctgggtcgc attgccaggg acgttgatgc gctgctgttc gtcgacatgg cccacatcgc
     3301 agggttagtt gcggccgggc ttcacccgag ccccttccca cacgccgat
//
LOCUS       DATLCO010000319         4064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_714029, whole genome shotgun sequence.
ACCESSION   DATLCO010000319 DATLCO010000000
VERSION     DATLCO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714029"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VMZ33_07345"
     CDS             <1..1041
                     /locus_tag="VMZ33_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR17084.1"
                     /translation="QTLLQLETVPAGQWSALLTRVAVSYALWRGELEDARNAAEQGWE
                     RVLETNDPTQTAQAASTVLEVCAASAERGRLKRDWSEVAAASELAARVLSGAEAQVAE
                     WDLPRGIGARREAELHLATARGHAARVRGRTQAETWSQLAEAWAKVPVPYQVAKARWW
                     QAQAALPTRSRRSEARRALQEAWKIARDLPAAPLQRALLDISLRGRITLPDANVVAIP
                     IEPEQRELVAVGPGPAPGATGADASKPDFGSRFGSAPASGLSPAESRFGLSPRENGVL
                     MVLTEGRTNREIAERLFISERTVAVHVRRILSKLGVSGRTEAAGLAIRLGLVPSEAPP
                     AQARPSARSGRS"
     gene            1055..1543
                     /locus_tag="VMZ33_07350"
     CDS             1055..1543
                     /locus_tag="VMZ33_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HUR17085.1"
                     /translation="MNKTTPRTSAGLRAAGVGLLTLVLAACAGGSGAAWTYAPLGPTA
                     DANASPTAGPSGTPAASPGATIDVETPQENSLAFVPNTLEAPAGSTVLVNYNNNSNLP
                     HNIHFFAGGDSTAESLGATPIVTGPDAPESVSITTPDEPGDYYFWCDVHLQAMAGMLT
                     VQ"
     gene            1681..3390
                     /locus_tag="VMZ33_07355"
     CDS             1681..3390
                     /locus_tag="VMZ33_07355"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HUR17086.1"
                     /translation="MSSNPSPAIAAFLSFLFPGAGQVYAGESRRGFVWAIPMVLFILG
                     ALWLLLGGQNAMLGFVERERFLALLVLNVAFFLYHVAAMLDAYSVAQQERRRGFGSSY
                     SGVAPIVLAALVSLTIVIHGVPEVIGYQASSTLCNVFRCEGEKPPDVLPSNTPRPPST
                     PRPPSTTGPTTPGFTAPPEPSDTPEPSATPIGSGLPRTPPPTRAPLPPFNAPDWPDWA
                     QDGYLNILLAGTDSRSEDGVDDASLRTDTMLVLSMDIEAGKAALFSFPRNLCNAADGS
                     CGLGTRYPEWLTIPLSSEAIALPANQAQFPSGNYGGMALGWNYLNALWRYAAINPAQF
                     PGSEGIGPECSEMFECERGWKALTGVLTEMSGLQFDGIIAVNLKGFSSVVDNLPENCP
                     DDDQRVALTNANCYGGIWINATKAIHDDVYHTSTGELLVVDIPAGCGYFDSEMALAFS
                     RSRHETSDYDRARRQSYVLTQLRKQLDPLALLPHIPALLAVAEQNLFLTFQDTDIQWL
                     AQAASRVDADRIYREDFAPGSVNKLGSMQGMRDKFANIFSEPEPEPEPEPSDPGQRCP
                     PRN"
     gene            complement(3398..>4064)
                     /locus_tag="VMZ33_07360"
     CDS             complement(3398..>4064)
                     /locus_tag="VMZ33_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUR17087.1"
                     /translation="LADPSAVVFDLDGTLVDTVETRIESWLKTFEEIGVKAEHDHVAR
                     LIGADGKRLAKEVAQAAGKRIDDDRAEGVDKRSGEIYDEINTNPKPIAGVRQLLLALS
                     ASNLPWAIATSSRREQTTVSVAALKLPTAPRIVDGSHVKNAKPAPDLLVLAAQQLRTP
                     PATTWYVGDASWDMLAAKAAGMIGIGVAYGAVRGDGLREAGARVVTTHRSILADLRRR
                     GAL"
BASE COUNT          646 a         1453 c         1302 g          663 t
ORIGIN      
        1 cagaccctcc ttcagctgga gacggtgccg gccggacagt ggtcggcgct gctcacccga
       61 gtggccgtga gctatgcgct gtggcgtggc gagctcgagg atgcccgtaa cgccgccgag
      121 cagggttggg agcgcgtgct cgagacgaat gacccgacgc agactgcgca agccgcttcc
      181 acggtcctgg aggtctgcgc cgcttccgcc gagcgcggcc ggctgaagcg cgactggtcg
      241 gaggttgccg cggccagcga gctcgccgcc cgtgttttga gcggcgctga ggcacaggtc
      301 gcggaatggg atctgccgcg cggcatcggc gcgcggcgcg aggccgagct ccacttggcc
      361 acggcacgag gccacgccgc tcgggtccgt ggccgcaccc aggcagagac gtggtcgcag
      421 ctcgccgagg cgtgggccaa ggtgcccgtc ccgtaccagg tggccaaggc gcgctggtgg
      481 caggcacagg cggcactgcc gactcgctcc cgccggtcgg aggctcgccg cgcactccag
      541 gaggcgtgga agatcgcgcg cgacctcccc gccgcgcccc tccagcgggc actgctcgac
      601 atctcccttc gtgggcgcat caccctgccc gacgccaacg tcgtggctat cccgatcgag
      661 cccgagcagc gcgagctcgt ggccgttggc ccgggaccgg caccgggcgc caccggcgct
      721 gacgcatcga agcctgactt cggctcccgt ttcgggtcgg ccccggcctc cgggctctca
      781 ccagcggaga gccgcttcgg tctgagcccg cgagagaacg gcgtcctgat ggttctcacc
      841 gagggtcgca ccaaccgcga gatagctgaa cgtttgttca tcagcgagcg aaccgtcgcg
      901 gtccacgttc ggcggatcct gtcgaagctc ggggtgtcgg gccgcaccga ggcggcgggt
      961 ctggccatcc gccttggact cgtgccgtcc gaggctccgc cggcgcaggc ccgaccgtcg
     1021 gcacgttccg gccgctcctg aaacaatcag ggccatgaac aagacgaccc ctcggacatc
     1081 tgccggcctg cgcgccgccg gtgttggcct gctgaccctc gtccttgccg cctgcgccgg
     1141 tggctcaggc gcggcctgga cctacgcgcc gctcggtccg accgccgatg ccaatgcgtc
     1201 ccccaccgcc ggtccgagcg gcacgcccgc ggcctcgccc ggtgccacta tcgacgtcga
     1261 gacgccccag gaaaactcgt tggcattcgt gcccaacacc cttgaggcgc cggccgggag
     1321 cacagtgctc gtcaactaca acaacaacag caacctgcca cacaacatcc acttcttcgc
     1381 cggcggcgac agcaccgctg agtcgctcgg cgctacgccc atcgtcacag gccccgacgc
     1441 tcccgagtcg gtttccatca cgactcccga cgaaccgggc gactactact tctggtgcga
     1501 tgtccacctg caggcgatgg cggggatgct caccgtccaa taggcgaagt gggcgcaggc
     1561 gtagcacgcc gggccgcctg atcgtccttc tcgcgtaaac ttcgctcgct cccgccctcg
     1621 tgcccaagca agcgggaacc gtgcactcgc acctccgcta gaggtctgac gacccgaacc
     1681 atgtcttcca atccgtcgcc cgcgatagcc gcgttcctgt cgttcctgtt tccgggtgcg
     1741 ggccaggtct acgcaggcga gtcgcgccga ggtttcgtct gggcgattcc gatggtgctc
     1801 ttcatcctcg gcgctctgtg gctgctgctg ggcgggcaaa acgccatgct cggattcgtc
     1861 gagcgcgagc gcttcctggc gctgctggtg ctcaacgtgg cgttcttcct ctaccacgtc
     1921 gccgcgatgc tcgacgccta ctcggtggcg cagcaggagc gtcgccgcgg gttcgggtcg
     1981 agctacagcg gcgtcgcgcc gatcgtcctg gccgcgctcg tctcgttgac gatcgtcatc
     2041 catggagtcc ctgaggtaat cgggtaccag gccagctcga cgttgtgcaa cgtcttccgc
     2101 tgcgagggcg agaagccgcc ggatgtcctc ccgtcaaata cgccccgacc gccatctaca
     2161 cctcgaccac cctccactac aggtcccacg acgccgggct ttaccgcgcc gccggagccg
     2221 agcgacaccc ccgagccgtc ggctacgccc attggcagtg gtctaccgcg aacaccgccg
     2281 ccgacccggg cgcctctgcc gccattcaat gctccggact ggccggactg ggcacaggac
     2341 ggctacctga acatccttct cgccggaacc gactcgcgct ccgaggacgg cgtcgacgac
     2401 gccagcctcc ggactgacac gatgctcgtc ctgtcgatgg acatcgaggc cggaaaggca
     2461 gccctgttca gcttcccacg caacctgtgc aacgccgccg acggcagctg cggcctcgga
     2521 acacggtatc ccgagtggct gacgatcccg ctctcgagcg aggccatcgc cctccccgcc
     2581 aaccaggcgc agttcccgag tggcaactac ggcgggatgg cactgggctg gaactacctg
     2641 aacgcgctgt ggcgctacgc cgccatcaac cccgcccagt tcccaggttc agaggggatc
     2701 ggccccgaat gttccgagat gttcgagtgt gagcgcggtt ggaaggctct caccggcgtg
     2761 ctaacggaga tgagcggact gcagttcgac ggcatcatcg ccgtcaacct gaagggtttc
     2821 tcttccgtgg tcgacaacct gcctgagaac tgcccggatg acgaccagcg cgtggccctc
     2881 actaacgcca actgctacgg cggcatctgg atcaacgcca cgaaggcgat ccacgacgac
     2941 gtctaccaca cctctacggg cgagctcctc gtggtagata tcccggccgg ctgcggttac
     3001 ttcgacagtg agatggctct ggcgttctcg cgatcacgcc acgagacgtc cgactacgac
     3061 cgagctcgcc ggcagtcata cgtcctcacg cagctccgca agcagctcga tcccctggcg
     3121 ctgttgccgc acataccggc gctgctggcc gtggctgagc agaatctctt cctgacgttc
     3181 caggacacgg acatccaatg gctggcgcag gccgcctcgc gagtcgacgc agacaggatc
     3241 taccgagagg acttcgctcc aggcagcgtc aacaagctgg gcagcatgca gggcatgcgc
     3301 gacaagttcg cgaacatctt cagcgagccg gagccggaac cagagcctga gccgtcagat
     3361 ccgggccagc gctgtccgcc gcggaactag gtcgggccta gagggctcca cgtcggcgca
     3421 ggtcggcgag gatcgagcgg tgggtcgtca cgacgcgcgc accggcctcg cgtaggccat
     3481 cgccacggac ggcgccgtaa gcgacgccga tcccaatcat gcccgcagcc tttgcagcga
     3541 gcatgtccca gctcgcgtcc ccgacgtacc aggtggtcgc cggcggcgtc cggagctgct
     3601 gagcggctag caccagcaga tcgggtgcag gcttggcgtt cttgacgtgg ctgccatcca
     3661 cgatacgcgg cgctgtcggc agcttgagcg cggccactga cacggtggtc tgctcgcggc
     3721 gactcgacgt cgcgatggcc cagggaaggt tggatgcgct caacgcgagc agcagctggc
     3781 gcacgccggc tatcggcttg gggttggtgt tgatctcgtc gtagatttcg ccagaccgct
     3841 tatcgacgcc ctccgcccgg tcgtcgtcga tgcgcttgcc cgccgcctgc gccacctcct
     3901 tggccagccg ctttccgtcg gcgccgatca gacgcgcgac gtgatcatgc tcggccttga
     3961 cgccgatctc ctcgaacgtc ttgagccacg attcgatgcg cgtctcaacc gtatcgacca
     4021 gggttccatc aaggtcgaag acgactgcgg agggatcggc gagc
//
LOCUS       DATLCO010000320         6472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2203632, whole genome shotgun sequence.
ACCESSION   DATLCO010000320 DATLCO010000000
VERSION     DATLCO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203632"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VMZ33_07365"
     CDS             complement(<1..335)
                     /locus_tag="VMZ33_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17088.1"
                     /translation="MTTEEVADHHAADDQADSQRGPTAAQLRRFIKSRPYVPLHELRR
                     RFELNGELDEVTRVEVPEHGQIFVGLPPREAEFVASLVRDGDIGVELSQDPGAPIVVG
                     VFAMRPVTRS"
     gene            complement(352..1548)
                     /locus_tag="VMZ33_07370"
     CDS             complement(352..1548)
                     /locus_tag="VMZ33_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HUR17089.1"
                     /translation="MSTRSVSGARYAERIARAQQTLPPADASALLIGVGADLQWLTGY
                     AAHALERLTMLVIPARGPASLVLPRLERAAAETCTAAQAGHIELVTWEETEDPFLLVA
                     DLLSQTNSRPEVQIGALGGAWGNLSGLLVSDRLWATFLLRIQAVLPDAAFGLASTVLS
                     DLRAIKDKEEVDLLRKAAHAADQAITGTAHGRLVGRTEAEIANDVRERLIDAGHDDAS
                     FWIVASGPNSASPHHEPTNRVVSAGEPLLLDIGGIVEGYGSDITRTFWVAGGDNHGPT
                     DEYRNLYDVLQRAQRAQLDAVRAGLVASELDAIGRRQIDAAGFGDKFIHRTGHGIGLD
                     GHEDPYIVETNDRELEVGNAFSVEPGIYIDGRYGARIEDIVVCREDSGDILNEASRDL
                     VVVSGE"
     gene            complement(1545..1931)
                     /locus_tag="VMZ33_07375"
     CDS             complement(1545..1931)
                     /locus_tag="VMZ33_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17090.1"
                     /translation="MEASTQIPQPPVVILADDLIWSSRLVAAARNAEARPAAARSAAE
                     LQVMLTGAPSGTPVIVDLTGRGYDGVEQVRATVALGHRVLAVGQHEDLPTRKAALAAG
                     AVRVLSYNKLFTDGPSVVSAFLEGRL"
     gene            1994..2977
                     /locus_tag="VMZ33_07380"
     CDS             1994..2977
                     /locus_tag="VMZ33_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUR17091.1"
                     /translation="MTRDRALGIALVVISACGYGSGALFAKPAYGTGIDWLILSVWRF
                     TFAAIASWAWLAIWPNQRRALFGLTRRRVAVLILLGVFFILNSGTYFAALETVSASLA
                     ALITYMYPALVAVLTIRFGRRLEGRHAWFALALATAGVALAVGGIDPDAAPPPIGIAL
                     AIASPIIYSFWIVMAARFSGERRARSGSASDDDLALPPHESESPSSDQSEPAPTAAIM
                     ITATAVAWWIVALGAGRPVAPWQIPNEAWLPLLGVGIVSTAIALQGFYAGARRIGAAQ
                     AALVSTVEPVYTITLASILFGEMLTPVQLIGGVMVIGGVLLAQLGSRGRAS"
     gene            2974..3636
                     /locus_tag="VMZ33_07385"
     CDS             2974..3636
                     /locus_tag="VMZ33_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUR17092.1"
                     /translation="MKLRIERPGKIVCVGVNYRAHASEQNRESPDHPVLFAKFPTAFI
                     RDGEEIVWDPALTQAVDFEAELAVVIGRTARRVSEAEALDYVAGYTCLNDVSARDLQY
                     SDKQFVRGKSLDTFAPIGPWVVTPDEVGDPGSLRIRCLVNGEVMQDSNTNDLIFGVPQ
                     LISFCSQAFTLEPGDVIATGTPSGVGWFRDPKSMLKDGDDVVVEIERIGRLTNRCRIM
                     AT"
     gene            3633..4310
                     /locus_tag="VMZ33_07390"
     CDS             3633..4310
                     /locus_tag="VMZ33_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR17093.1"
                     /translation="MSTDGWTILVVDDDPAWVGALGAVLGKAGHRIVAAYDGDEALRR
                     FDADRVDAVLLDLAMPGLDGQEVCRQIRARSDVPILIVSGERDKSVPPELLDLGADDY
                     VRKGTPSAELLARIRAVVRRRGPGNQPRPTGDWDLDQRRHEIRWQSAAVPVTAIEFRL
                     LATLVERLHDLVPHHDLLVAGWPMENDPDPLWLKPHMARLRDKLRALGAPVPIAVRGV
                     GYRLDDT"
     gene            complement(4307..5539)
                     /locus_tag="VMZ33_07395"
     CDS             complement(4307..5539)
                     /locus_tag="VMZ33_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR17094.1"
                     /translation="MTRSRLRSRLSPYGGDLPASFWYLWFGTVINRLGGFAVPFLMLY
                     LTARIGITPQTAALVVSALGAGSFLAQLFGGELSDRLGRRPVMMLSFLVTPVAMLALG
                     IVHEVWMLVIATFVLGFFTDLFRPAMSAAIIDLVPADKRTRAFGYIYWAINLGAALAP
                     IAAGLLANFDYFLLFAGDALTTAIFGIIVLVRVPETQSIEIAEAARLRTTSRLGQALR
                     DPMLLVFVFLSLLVGLIYSQSHVTLPLEMAANGLPPSDYGLAIAVNGALIVLVTLHVA
                     RRVERLPRFAVMAVAGLLLGIGFGLTAFAGSLSAFALTVAIWTIGEVIGAAVAPVIVS
                     EMSPPEMRGLYQGVWGSSWGLAFFLGPALGGFVFGTYGSDVLWASMFALGVLLFIGYL
                     LLSIPARRRAQAVAAQAG"
     gene            complement(5938..>6472)
                     /locus_tag="VMZ33_07400"
     CDS             complement(5938..>6472)
                     /locus_tag="VMZ33_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR17095.1"
                     /translation="LKALEQVERLDQTVDSILASLRVLQPNTTQPVRFDLADAVDDTA
                     RELAPIFRKQRLVATFLERPLWTLASVEQLRRLLGYLLENASKYAPAEGAIDLYGWRA
                     DGRVFLAITDDGPGIPRDWREKIFEPFVRLDDSPRGAGIGLFAARHLARSMKGDLRAE
                     DREPRGSQFVLELPAAR"
BASE COUNT         1076 a         2160 c         2185 g         1051 t
ORIGIN      
        1 gacctggtga cgggcctcat cgcgaatacg ccgacgacga tgggggcgcc cgggtcctgc
       61 gacagctcta cgccgatatc gccgtcgcgg acgaggctcg ccacgaactc agcctcgcgc
      121 ggcggcagac ccacgaagat ctggccgtgt tccggcactt cgacgcgggt cacttcgtcg
      181 agctcgccgt tgagctcgaa gcggcggcgc agctcgtgca gcggcacata cgggcgcgac
      241 ttgatgaagc gccgaagctg ggcagcggtc ggaccgcgtt gtgaatcggc ctgatcgtcc
      301 gctgcgtgat ggtcggcgac ttcttcagtg gtcatgggtg tcatgaccag cctactcgcc
      361 ggaaacgacg acgaggtcgc gcgacgcttc gttcaggatg tcgccgctgt cctctcgaca
      421 gacgacgatg tcctcgatgc gagctccgta gcggccatcg atgtagatgc ccggctcgac
      481 actgaaggca ttgccaactt cgagctcgcg atcgttggtt tcgacgatgt acggatcctc
      541 gtgaccgtcg aggccgatgc cgtgtcccgt gcgatgaatg aacttgtcgc cgaagcccgc
      601 ggcgtcgatc tggcgacggc cgatcgcgtc cagctcgctc gccacgaggc ccgcgcggac
      661 ggcgtcgagc tgcgcccgct gcgccctctg gaggacgtcg taaaggttgc ggtattcgtc
      721 ggtcgggccg tggttgtccc ctcccgcgac ccagaacgtg cgggtgatgt cggagccgta
      781 gccctcgaca atcccgccga tgtcgagcag cagtggctca ccggcgctga cgacgcgatt
      841 tgtgggctcg tgatggggcg aggctgagtt cgggccactg gcaacgatcc agaaagaggc
      901 gtcatcgtgg ccggcgtcga tcaggcgctc gcggacatcg tttgcgattt ccgcctccgt
      961 tcgccccacc aaccgcccgt gcgccgtccc ggtgattgcc tggtctgcgg catgtgccgc
     1021 cttccgcagg agatcaacct cttccttgtc cttgatcgcg cgcaggtccg agagcacggt
     1081 cgatgccagc ccgaacgcag cgtcaggcag taccgcctgg atgcgcagca ggaaggtcgc
     1141 ccacagccga tccgagacga gaagtccgct cagattcccc caggcaccac ccagcgcgcc
     1201 gatctgcacc tccggacggc tgttggtctg gctcaggaga tcggcaacga gcaggaaggg
     1261 gtcctccgtc tcttcccacg ttacgagctc gatgtggcct gcctgtgcgg ctgtgcaagt
     1321 ttcggcggcc gcgcgttcaa gtcgcggcag gaccagggat gcgggaccgc gcgccgggat
     1381 cacgagcatc gtcagccgct cgagggcatg tgcggcgtat cccgtcagcc actggaggtc
     1441 ggcaccgacg ccgatcagca gggccgaggc gtcagccggt gggagtgtct gttgggcgcg
     1501 ggcgatccgc tcggcgtagc gtgcgccgct cacagaacgt gtgctcacaa tcggccctcg
     1561 aggaacgcgc tcacgaccga cgggccgtcc gtgaagagct tgttgtacga aaggacgcgg
     1621 acggcgccgg cggcgagcgc ggctttgcgt gtcgggaggt cctcatgctg cccgacggcg
     1681 agcacgcgat ggccgagggc gacggttgcc cggacctgtt cgacgccgtc gtagccacga
     1741 cctgtgaggt cgacgatgac cggggtgccg gagggcgcgc cggtgagcat cacctgtagt
     1801 tcagcggctg atcgcgcggc ggcgggtctt gcctcggcgt tcctggctgc ggcgacgaga
     1861 cgcgacgacc agatgaggtc gtcggcgaga atcacgacag ggggttgcgg gatctgggtc
     1921 gaggcttcca cggctcgccg agtgtatcga cgcgtgcccc attagcatcc gccgccatgc
     1981 ccgagtcgaa gtcttgacac gcgatcgcgc cctcggcatc gcgctggtcg tcatctcggc
     2041 ctgtggctac ggctcaggcg ctctgttcgc caagcccgcc tacggcaccg ggatcgactg
     2101 gctgatcctg tcggtttggc gcttcacatt cgcggccatc gcgtcgtggg cgtggctggc
     2161 tatctggccc aaccagaggc gagcgctctt cggcctcacg cgccgccgag tggccgtcct
     2221 gatcctcctc ggcgttttct tcatcctcaa cagcgggacg tacttcgccg cgttggaaac
     2281 cgtgtcggcg tccctggccg ctctcatcac gtacatgtat ccggctctcg tggcggtgct
     2341 gacgatccgc ttcggacgcc gccttgaggg tcgccacgca tggttcgcgc tggcgctcgc
     2401 gacggcgggc gtggcgctgg cagtgggcgg catcgacccc gacgcggcgc cgccgccgat
     2461 aggcatcgcc ctggcgatcg cctcacccat catctactcg ttctggatcg tgatggcagc
     2521 acggttcagc ggtgagcggc gggcccgcag cggatcggct tccgatgacg accttgcgct
     2581 ccctccgcac gagtccgagt ctccctccag cgaccagtcg gagccggcgc cgaccgccgc
     2641 aatcatgatc accgcgaccg ccgtcgcgtg gtggatcgtg gcactcggcg ccggcaggcc
     2701 cgtggcaccg tggcagattc ccaacgaggc gtggctgccg ctgctcggcg ttggcattgt
     2761 ctcgaccgcc attgcgctcc agggctttta cgccggagcg cggcggatcg gagctgcgca
     2821 ggccgccctt gtgagcaccg ttgagcccgt ctacacgatc acgcttgcct cgatcctgtt
     2881 tggtgagatg ctgaccccag tgcagctcat cggcggcgtg atggttattg gtggagtgct
     2941 cttggcccag ctcggctcgc gcggacgagc ttcgtgaagc ttcggatcga acggcccggg
     3001 aagatcgtct gcgtcggcgt gaactaccgc gcccacgcga gcgagcagaa ccgcgaatcg
     3061 ccggaccacc cggtcctctt cgcaaagttt ccgacggcct tcatccgcga cggtgaggaa
     3121 atcgtctggg atcctgccct gacgcaggcg gtcgacttcg aggccgagct ggcggtcgtt
     3181 atcggccgga cagcacgccg cgtgagcgaa gccgaagccc tcgactacgt ggctggctac
     3241 acctgcctga acgacgtcag cgcgcgcgac ctgcagtaca gcgacaagca attcgtacgc
     3301 ggcaagtcgc tcgatacctt cgcgccaatc ggcccgtggg tcgtgacgcc ggacgaggtc
     3361 ggcgacccgg gctcactgcg tatccgctgc ctcgtcaacg gcgaggtgat gcaggactcg
     3421 aacacgaacg acctcatctt cggggtgccg cagctgatca gcttctgctc gcaggcgttc
     3481 acgctcgagc ccggggacgt catcgccacg ggcacgccga gcggcgtcgg ctggttccgg
     3541 gacccgaaga gcatgctcaa ggacggggac gacgtggttg tcgagatcga gcggatcggc
     3601 cggctgacca accggtgccg aatcatggcg acatgagcac ggacggctgg acgatcctgg
     3661 tcgtcgacga cgacccggca tgggtcggtg ccctcggcgc ggtcctgggc aaggccggtc
     3721 atcggatcgt cgccgcgtat gacggtgacg aggcgctgcg tcgcttcgat gccgaccggg
     3781 tcgacgccgt gttgctcgac ctggcgatgc ccgggctgga tgggcaggag gtgtgtcgcc
     3841 agatccgtgc cagatcggac gtgccgattt tgatcgtctc gggcgagcgc gacaaatcgg
     3901 tgccgccgga gctcctcgat ctcggcgccg acgactacgt tcgcaagggc acgccctcag
     3961 ccgagctgct ggcgcgcatc cgcgccgtcg ttcgtcgccg tggcccgggc aatcagccgc
     4021 ggcccacggg cgactgggat ctagatcagc gtcgtcatga gatccgttgg cagagcgccg
     4081 ctgtgccggt gaccgccatc gagtttcggc tgctggcgac gcttgtcgag cgactccacg
     4141 acctcgtgcc gcaccacgac ctgctcgtag ccggctggcc gatggagaac gaccctgatc
     4201 cgctgtggct caaaccgcac atggcgcgcc tacgcgacaa gctgcgtgcg ctaggtgcac
     4261 cggtgccaat cgctgtccgc ggcgtcggtt atcggctgga cgacacctag cctgcctgcg
     4321 cggctactgc ctgcgcacgt cggcgagcgg ggatcgagag caagaggtag ccgataaaga
     4381 gcaggacccc gagggcgaac atcgatgccc acaggacgtc cgatccatag gtgccgaaga
     4441 caaatccgcc aagcgccggg ccgaggaaga aggcgagccc ccacgacgat ccccacactc
     4501 cctggtagag gccgcgcatc tccggcggcg acatctcgct gacgatcacc ggggcaacgg
     4561 cagcgccgat tacctcgccg atggtccaga tggcgacggt cagcgcgaag gccgacaggc
     4621 tcccggcgaa tgcggtgaga ccgaaaccga tcccgagcag caagccggcc acggccatga
     4681 cggcgaagcg cggcagccgc tccacccgtc gcgcgacgtg gagcgtgacg aggacgatga
     4741 gcgcgccgtt gacggcgatg gccagtccat agtcgctcgg cggcaggccg ttcgcggcca
     4801 tctccaaggg gagtgtcacg tggctctgcg aatagatgag cccgaccagg agcgagagga
     4861 agacgaagac gagcagcatc gggtcgcgca gcgcctggcc aagacgtgac gtggtccgca
     4921 gccgtgccgc ttcggcgatc tcgatcgact gcgtctcggg cacgcgaacc aggacgatga
     4981 tcccgaatat cgccgtagtc agggcgtcac cggcgaacaa caggaagtag tcgaagttcg
     5041 ccaggaggcc ggcggcaatc ggcgccagcg ccgcgccgag gttaatcgcc cagtagatgt
     5101 agccgaatgc ccgcgtgcgc ttgtcggccg gaaccaggtc gatgatcgcg gcactcatcg
     5161 ccggcctgaa caggtcggtg aagaagccca acacgaacgt cgcgatgacc agcatccaca
     5221 cctcgtggac gattcccagt gcgagcatgg ccacaggcgt cacgaggaag ctgagcatca
     5281 ttaccggacg ccgaccgagg cggtccgaca gctccccgcc gaaaagctgc gccagaaagg
     5341 agccggcacc aagcgccgaa acgacgagcg ccgcggtctg gggcgtgatc ccgatgcgcg
     5401 ccgtcaggta gagcatcagg aacggcacag caaatccgcc cagccggttg atgaccgtcc
     5461 cgaaccacag gtaccagaac gaggctggga ggtcgccgcc gtagggcgat agccgagatc
     5521 ggagtcgcga tcgcgtcatc gagcgaaaga taacctcttg cgcagcgtct gtgggttacc
     5581 caccgatcgc aaccgtgccg atacccaacg acaacgccac tgccgccgac acgggcatcc
     5641 tcaattccta atctaggaac atgagcgact cgtccgttac ccgattcctt ccaccgcgag
     5701 acgccaccgg acccgttacc tgcgcggtct gcggctgccg gctcgaggca ctggccgggg
     5761 ctgaggacgg ttcgtatcgc catttcgcgt cgctcatgcc tgggcaggac gcgcgcggct
     5821 gtcgccctca ctgcgttgac gccgttcacg accaagccgg acgggtgacc cacgaggcgg
     5881 ccgcggcctg acgcctcgta ccggagatcg cgcgctgagt ccagccaacc cggcggccta
     5941 gcgagcggcg ggtagctcga gcacgaactg actgcctctc ggctcgcgat cctcggcacg
     6001 caggtcgccc ttcatcgagc gcgccagatg ccgcgccgcg aataggccaa tgcctgcgcc
     6061 gcgcggcgag tcatcgagcc tgacgaacgg ctcgaagatc ttctcgcgcc agtcgcgtgg
     6121 gatgcccggc ccgtcgtccg tgatcgccag gaatacccgg ccgtccgccc gccagccgta
     6181 caggtcgatt gctccctccg caggggcgta cttcgaagcg ttctcgagca ggtaacccag
     6241 caggcgccgc agctgctcca ccgacgccag cgtccagagc ggccgctcga ggaaggtggc
     6301 cacgagcctc tgcttccgaa agatcggggc cagctcccgg gcggtgtcgt ctactgcgtc
     6361 ggcgaggtcg aacctgaccg gttgcgtcgt gttgggctgg aggacgcgca ggctggccag
     6421 aatcgagtcc acggtctgat cgaggcgctc gacctgctcc agcgccttca gc
//
LOCUS       DATLCO010000321         4473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8556660, whole genome shotgun sequence.
ACCESSION   DATLCO010000321 DATLCO010000000
VERSION     DATLCO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8556660"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..971
                     /locus_tag="VMZ33_07405"
     CDS             411..971
                     /locus_tag="VMZ33_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HUR17096.1"
                     /translation="MSPEVFVSVDIEASAQSPGVGSLLSIGACLVDRPDIGIYLELKP
                     VPGRAWQTSAEEVHGLSRERLEQEGLEPAQAMEQLDRWLREVCAEGRPVFVGFNAPFD
                     WMFVADYFYRYVGRNPFGISALDLKSYYMGRAAVDTWEATRRVNIDERLGLAPDHTHQ
                     ALEDARGQAALARVLLRGTLERGAEG"
     gene            1091..3178
                     /locus_tag="VMZ33_07410"
     CDS             1091..3178
                     /locus_tag="VMZ33_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR17097.1"
                     /translation="MRNRWHAVRRRVAQRPRLWLAGGLIAAILLVVGSAFVFGQSKPT
                     NEAIRPTTTERWTLVELTSQIEAGNVLTITVGAGDSNTPMLLAQNDSRQYVAISVNGT
                     IADATSALASLGYRDLLSPVALSTAESRTASGGGWDAIRIITTLVMLGTVIGLGVWMI
                     VRARGSRISWRGVSSGKFATVMPAEKRQSSSPTLLAASPQAAPAGSADPVRLADVAGC
                     DEAKLELSEAIDFLRDPERFRDLGARVPRGIMLYGPPGTGKTMLARAVATEADVPFHH
                     ASGSDFVEKYVGMGARRVRDLFAQSRKLGKGVIFIDEFDALGKARGGPNSHEEREQTL
                     NQLLVELDGFGTSADIVVIAATNRLDTLDDAVLRPGRFSRKVHVGLPDVGGRKAILDV
                     HSRGKPLGHEVDIELLARKTYGFSGAQLADLLNEAAIMAARRSGDTITNEDLHNGWLK
                     VAVGTGRRRSMTERDRAIIAAHEVGHALCGKVHGDTRRVEEISLFAHGEALGVTVSSQ
                     EDNDLPAESDLRARLVALMGGRAAEELLFHQVTPGAANDFEKATQIATSMVHRWGMGR
                     DPRAAEFGTSGRGTLSFIAAKRDGTLPTELQAAATRAIAAILDEAYDTARNTLIDKLD
                     ILRRIGVYLVQQERIDGDTFDALYEGKLDVPDAQSEWRPAAARPREWAAITAMAISNE
                     KFNEGSVGASSST"
     gene            complement(3404..4186)
                     /locus_tag="VMZ33_07415"
     CDS             complement(3404..4186)
                     /locus_tag="VMZ33_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HUR17098.1"
                     /translation="MGLLDGKKALIFGVANDHSIAWGIAKAFHEHGAQIGFSSLEMMI
                     DKRVRPLADSIGATFVEPSDVQKDEDIERVFSRWSDAYGSLDILVHALAFAKKEDLEG
                     RFMDTSRDGFALALDISAYSMVALAQRAAPLMTNGGSMLTLSYYGAEKVVANYNVMGV
                     AKAALEASVRYLANDLGPDGIRVNAISAGPIRTLAASGVANFRKMYGIFPDLAPLRRN
                     VTLDDCGNTAVYLASDLSSGVTGETIYVDAGFNILGVPNPES"
     gene            complement(4212..>4473)
                     /locus_tag="VMZ33_07420"
     CDS             complement(4212..>4473)
                     /locus_tag="VMZ33_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17099.1"
                     /translation="DWTLQLRTVPAAPVYDGSGTHGALIGSVAVPDGLAAGQSVELTV
                     NATAPAEAGSWLVKSDVRLVDDTLTSDLGVVALQVGLTTTSP"
BASE COUNT          781 a         1496 c         1431 g          765 t
ORIGIN      
        1 cgctcggaac gagtcccggg cgattgacct gagagcaagc tctctggtcg aaccttcctt
       61 ccggccgact cagtggcttt ggcttgtgac cgccgctcct gtgtcggttt ccgtggcccc
      121 ttcgggctga gacgaacgtt aaggtcgccc cgacccggac acaataggcg ccgtccacga
      181 ctttgccgga tcttttcggc tttatccccg acgttgccga cgtgtgtgga tagtcgttgt
      241 ggaaaggctg ccctagaaga tcgcccgcgt gcattgcacc agcacgacga tgacgatcac
      301 gatcccgacg agaatgccga tgatgaactt gcccaagccg acagcatcca tcccgtgccg
      361 cagcgagcga gccagtctga tcgtccttca cgtttgcacg ttgcttctct gtgagtcctg
      421 aagtcttcgt gagcgtggac atcgaggctt ccgctcagag cccaggcgtc ggcagcctgc
      481 tgtcgatcgg ggcatgcctt gtggatcggc cggatatcgg catatacctc gagttgaagc
      541 cggtgcccgg acgagcctgg caaacgtcgg cggaagaagt ccacggcctg agcagggagc
      601 ggctggagca ggagggactt gagccggcgc aggcaatgga gcaactcgac cgttggcttc
      661 gcgaagtctg cgcggagggc cggccggtct tcgtcggctt caacgctccc ttcgactgga
      721 tgttcgtggc cgattacttc tacaggtatg tcggccgaaa tccattcggc atcagcgcgc
      781 tcgacctcaa gtcgtactac atggggcgcg ccgccgtgga cacctgggag gcaacgcggc
      841 gggtcaacat cgacgagcgt ttgggcctag cgccggacca cacgcatcaa gccctggagg
      901 acgcgcgcgg gcaggcggcg ctggctcgtg ttttgttacg tggcacgctg gagcgcgggg
      961 cagaaggtta gtgtgaacct ctcattcgta tatcagtttt ccatgtagga tgcgccgcac
     1021 atcaggtggg ggcctgaacg ggggcaggta gctgcgcagg tcacagaccc caaccttcag
     1081 tggggtaacg atgcgcaaca gatggcatgc cgttcggaga cgggtcgcac agcggccgcg
     1141 gctctggctg gccggcggcc tcatcgctgc catcctcctc gttgtcggat ccgcattcgt
     1201 ctttgggcag agcaagccga cgaatgaagc catccgcccg accacgaccg agcgctggac
     1261 gctcgtcgag ctgacctcac agatcgaggc tggcaacgtc ctgaccatca ctgtcggcgc
     1321 tggcgactcc aacacgccga tgctccttgc ccagaacgac tcgcgccaat acgtcgcgat
     1381 cagcgtcaat ggaacgatcg ccgacgccac gtcggccctg gccagcctcg gctaccgcga
     1441 cctcttgtcg ccggtcgcgc tatccacagc ggagtcccgc actgccagcg gcggtgggtg
     1501 ggatgcaatc cgcatcatta ccacgctcgt catgctcggg accgtcatcg gtctcggcgt
     1561 gtggatgatc gtccgcgcac gcggcagccg tatctcttgg agaggggtca gctccggcaa
     1621 gttcgcgacg gtcatgcccg ccgagaagcg gcagtcctca agcccgacgt tactcgccgc
     1681 gagcccgcag gctgcacctg cgggctcagc ggatcctgtc cgcctcgccg acgtcgccgg
     1741 ctgcgacgaa gccaagctgg agctgtccga ggcgatcgac ttcctgcgcg accccgaacg
     1801 cttccgcgac ctgggcgcgc gcgtgccacg cggcatcatg ttgtacgggc ctcctggtac
     1861 cggcaagacg atgctcgcgc gcgcggtagc gacggaggcc gacgtcccgt tccaccacgc
     1921 gtccggttcc gacttcgtcg agaagtacgt aggcatgggc gcgcgacgcg tacgcgacct
     1981 gttcgcgcag tcgcggaagc tgggcaaagg cgtgatcttc atcgacgagt tcgacgccct
     2041 gggcaaggcc cgcggcgggc ccaattccca cgaggaacgg gaacagacgc tcaaccagct
     2101 gctggtcgag ctcgacggct tcggtacgag cgccgacatc gtggtcatcg ccgcgaccaa
     2161 caggctggat accctcgacg atgcggtcct ccgccccggc aggttctcgc gaaaggtcca
     2221 cgtcgggctg ccggacgtcg gcggtcggaa ggcgattctc gatgttcact cgcgcggcaa
     2281 gccgcttggg cacgaggtcg atatcgagct gctggcccgc aaaacctacg gcttctcagg
     2341 cgcgcagctg gccgacctgc tcaacgaggc cgcgatcatg gccgcccgtc gcagcggcga
     2401 cacgatcacg aacgaggatc tccacaatgg ctggctgaag gtcgccgtcg gcacaggccg
     2461 ccgccgctcg atgaccgaac gcgaccgggc gatcatcgcc gcccacgagg tcggccacgc
     2521 gctctgcggc aaggtccacg gcgacacgcg gcgtgtcgaa gagatctcgc tgttcgccca
     2581 cggtgaggca cttggcgtca cggtctcgtc ccaggaggac aacgacctgc cggcggagtc
     2641 agatcttcgt gcgcgcctcg tcgcgctgat gggcggccga gccgccgagg agctgctctt
     2701 ccaccaggtg acgcccggcg ccgcgaacga cttcgagaag gccacgcaga tcgccacgtc
     2761 gatggttcac cgctggggca tgggccgcga tccccgcgcc gccgagttcg ggacctccgg
     2821 ccgcggcacc ctgtcgttca tcgccgccaa gcgcgatggg acgttgccga ccgagctgca
     2881 ggccgctgcc acgcgcgcga tcgccgcaat cctggacgag gcatacgaca cagcgcgcaa
     2941 cacgctcatc gacaagctcg acatcctgcg ccggatcggc gtctacctgg tccagcagga
     3001 gcgtatcgat ggcgacacgt tcgacgcgct gtatgagggc aagctggacg ttcccgacgc
     3061 ccagagcgag tggcgacccg ccgcggctcg tccccgtgaa tgggcagcga tcaccgcaat
     3121 ggcgatctcg aacgagaagt tcaatgaggg ctcggtcgga gcctcgtcga gtacctgacg
     3181 ttgggcgact gctcgctcac cgagcgtccc gtcgaacgag gccttcactc cgctctgggg
     3241 acgtttggcg gagcatcgcc tcgctgatcg ggtcagttgt ccgcggaacg cggtcaccat
     3301 ccttggccca aggcgcccgg cggacgcgtt actcgcccag cgagcatcaa ctgccgactc
     3361 acaggccggc ggtcacggcg tcccgcgacc cgtcacgccg ctctcaggac tcggggttgg
     3421 gcactcccag gatgttgaaa ccggcgtcga cgtagatcgt ctccccggtc acacccgacg
     3481 acaggtcgct cgccaggtag acggccgtgt tgccgcagtc gtcgagcgtg acgttccgtc
     3541 gcagcggcgc gaggtcgggg aaaatcccgt acatcttccg gaagttcgcc acgccacttg
     3601 cggccagcgt tcgaatgggt cccgcggaaa tcgcgttgac ccggatgccg tccggaccga
     3661 ggtcgttggc gaggtaccgc accgaagctt ccagagcggc tttggcaacg cccatcacgt
     3721 tgtagttcgc gacgaccttt tcggcgccgt agtaggacag cgtcagcatt gagccgccat
     3781 tggtcatcag cggcgcagcc cgctgggcga gcgcgaccat cgagtacgcg gaaatgtcca
     3841 gcgccagcgc gaacccatcg cgtgacgtgt ccatgaaacg tccctcgagg tcttcctttt
     3901 tcgcgaacgc cagggcgtga acaaggatgt ccaacgatcc ataggcatcg ctccagcgtg
     3961 aaaagacgcg ctcgatgtcc tcgtccttct ggacatcgct cggctcgacg aacgtcgcac
     4021 caatggaatc cgccagcggc ctgacccgct tgtcgatcat catctcgagc gagctgaatc
     4081 caatttgggc gccatgctcg tggaatgcct tggctatgcc ccacgcgatc gagtggtcat
     4141 tggctacgcc gaagatcagc gccttcttgc catcaagcag tcccatgagc gccgacgata
     4201 tcagcccagc gtcagggcga ggtcgtggtc agcccgacct gcagcgctac gacacccagg
     4261 tccgacgtca gagtgtcgtc tacgagtcgg acgtccgact tcaccagcca gctgcctgcc
     4321 tccgccggcg cagtggcgtt gaccgtaagc tcgaccgact gcccggctgc caggccatcc
     4381 ggcacggcca cggacccaat caacgctccg tgggtgcccg agccgtcgta caccggcgcc
     4441 gcgggaacgg tgcgcaattg cagagtccaa tcg
//
LOCUS       DATLCO010000322         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_5931454, whole genome shotgun sequence.
ACCESSION   DATLCO010000322 DATLCO010000000
VERSION     DATLCO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5931454"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VMZ33_07425"
     CDS             complement(<1..375)
                     /locus_tag="VMZ33_07425"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HUR17100.1"
                     /translation="MDRNLPDRVQQLVDEIENEVGEDGFAISANLTAEISDALQNGSH
                     DSGRDGQGSIEAAVREILTEIGEDPDRQGLERTPDRVRRMYHELTAGYHVDPERLVNS
                     AIFDVDYSEMVVVKDISFYSLCE"
     gene            470..937
                     /locus_tag="VMZ33_07430"
     CDS             470..937
                     /locus_tag="VMZ33_07430"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HUR17101.1"
                     /translation="MSVDLSLQERYGRHTICFGCGPANEKGLHIRSFADGDEVVLEWH
                     PEAHHQAFENVLNGGIIGVLFDCHSNWTAAHHLMHARGADKPPTTVTADFHVRLLKPT
                     PIDGPVTIRARVVDATDDRATVEAEMTADGLVTATCRATFVAVGPGHPAYDRW"
     gene            complement(922..1884)
                     /locus_tag="VMZ33_07435"
     CDS             complement(922..1884)
                     /locus_tag="VMZ33_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR17102.1"
                     /translation="MAHDPDLSADLPPDRSPDSLVVTTDTGDQIHYLDWRAPAETNGL
                     PPLLLIHGLSATAGVWAPIARRLAAQTHLLAVDLRGHGLSDSPRSGYELESLAYDALT
                     VLSANGYGTAVGGPPSAVAGHGLGAMVAATMAALEGPSVAAIALVDGGWEDLREATGQ
                     EPAEFERTIGDPPEITRSMDAYLADRREFDPATWDAEQERAARAAVDEKPAGHVVAVL
                     RRHASHATVESMFDYRPTDTLTQIKAPVLAAIAEAGTADDETVRERLLALDDINRDRA
                     AAGLPAVDIRRFPGVGHNLMRYRPAELSAALLELLRLAQRHQRS"
     gene            1902..>2351
                     /locus_tag="VMZ33_07440"
     CDS             1902..>2351
                     /locus_tag="VMZ33_07440"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HUR17103.1"
                     /translation="MPLKRRGTQARRRPGRRVAIPAAPEYLHQPFEALVEMALDTLPA
                     AARRLLENVAIVIEDEPSDEQLRASETPPTESLYGLYEGVSGVFYASEWAQLPNKISI
                     FRLPLEEDFPDPEELAHEVQETILHELGHHAGLDESGLREVGRYVRRN"
BASE COUNT          389 a          788 c          767 g          407 t
ORIGIN      
        1 ctcgcacagg ctgtagaaag agatgtcctt tacgacgacc atctcgctgt aatcgacatc
       61 aaagattgcg ctattgacga gccgctcggg atccacgtgg tagcccgccg tcagctcgtg
      121 gtacatgcgc cgaactcggt ccggggtgcg ctccaggccc tgccggtcag ggtcctcccc
      181 gatctccgtc aggatctcgc gcacagcggc ctcgatggaa ccctggccat cccgacctga
      241 gtcgtggctc ccattttgga gcgcgtcgga gatctcggca gtgaggttcg cgctgatcgc
      301 gaaaccgtct tcgccgactt cgttctcgat ttcgtcgacc aactgttgga cgcgatcggg
      361 caagttccgg tccatggttt gcgattctga cacggcatag actcgcgctc atgttgagtg
      421 ttgccgctgc gctcgccgcc acttgcccga tcacctttgc ggcaacccct tgagcgtcga
      481 tctcagcctc caggagcgct acgggcggca cacgatctgc tttggctgcg ggccggccaa
      541 cgaaaagggc ctgcacatcc gctcattcgc ggacggagac gaggtcgtgc tcgagtggca
      601 ccccgaggct catcaccagg ccttcgagaa cgtcctgaac ggcggcatca tcggcgtgct
      661 tttcgactgc cactcgaact ggacggctgc ccaccactta atgcatgcgc gcggagccga
      721 caaaccgccc accacagtga cggctgactt ccacgtccga ctgctcaagc cgactccaat
      781 cgacgggccg gtgacgatcc gagcccgagt cgtcgacgcg accgacgacc gcgcgaccgt
      841 cgaagcggag atgaccgccg acggcctagt gacagccacc tgccgggcaa cgttcgtggc
      901 cgtgggcccc ggccatcccg cctacgaccg ctggtgacgc tgggccaggc gaagcagctc
      961 gagaagagct gcgctgagct ccgcggggcg gtagcgcatg aggttgtggc cgacgcccgg
     1021 gaaccgtcgg atgtcgaccg ctgggagccc agccgcggcc ctgtcgcggt tgatgtcgtc
     1081 aagggccagc agtcgctcgc gcacggtctc gtcgtcggcc gttcccgcct ctgcgatcgc
     1141 agcaaggacc ggtgccttga tctgggtcag agtgtctgtc gggcggtagt cgaacatcga
     1201 ctccactgtg gcgtgcgagg cgtgccttcg taggaccgcc accacgtggc cggcgggctt
     1261 ttcgtcgacc gcggcgcggg cggcgcgctc ctgttcggca tcccaggtcg cggggtcgaa
     1321 ttcccgtctg tcggccagat aggcgtccat cgaccgggtg atttcgggcg gatcgccgat
     1381 cgtgcgctcg aactcagcgg gctcctggcc ggttgcctcg cgcaggtctt cccagccgcc
     1441 gtcgaccaat gcgatggccg cgaccgacgg gccctccagc gctgccatgg tcgcggcgac
     1501 catcgcgcca agaccgtggc ccgccaccgc gctcggcgga ccgccgactg ctgtgccgta
     1561 gccgttcgcc gacagcacgg tcagcgcgtc ataggccaac gactcaagct cgtaaccgct
     1621 cctcggcgag tcggaaaggc cgtggcctcg caggtccacc gcgagcaggt gcgtctgcgc
     1681 agccagccga cgagcgattg gtgcccaaac ccctgccgtc gcactcaggc cgtggatgag
     1741 cagaagaggc ggcagaccat tggtctccgc gggagctctc cagtcgaggt agtggatctg
     1801 atcgccggtg tcggtcgtga cgaccaacga atcgggcgat cgatccggcg gtaggtcggc
     1861 ggataggtcg ggatcgtgcg ccacgtcgat agcataggaa cgtgcccttg aaacgccgcg
     1921 ggacgcaggc tcggcgccgg ccggggcgtc gcgtcgcgat cccggccgcg cccgagtacc
     1981 ttcaccagcc cttcgaggca ctggtcgaga tggccctcga tacgctcccc gcagccgcgc
     2041 gacgactgct tgaaaacgtc gcgatcgtaa tcgaggacga gccgtccgac gagcagctgc
     2101 gcgcgagcga gactccaccc acggagtcgc tttacgggct gtacgaaggc gtgtcgggcg
     2161 tgttttacgc ctccgagtgg gctcagctgc ccaacaagat ttcgatcttc cgtctaccac
     2221 tcgaagagga ctttccggat ccggaggagc tcgcgcacga agtccaggag acgatcctcc
     2281 acgagcttgg acatcacgcc gggctcgatg agagtggttt gcgcgaggtc gggcgttacg
     2341 ttcgcaggaa c
//
LOCUS       DATLCO010000323         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_4430909, whole genome shotgun sequence.
ACCESSION   DATLCO010000323 DATLCO010000000
VERSION     DATLCO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4430909"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VMZ33_07445"
     CDS             <1..1072
                     /locus_tag="VMZ33_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUR17104.1"
                     /translation="SWLLAFILSPLVGLILRAFPRLPRVVVVGVVYGGLFIGLSAIIL
                     VVAGSLAASIGSFITDLPELQAKLPEILAGIQGGLNNLGFQVDLVAAAQNLFAGLGDL
                     GNDLVRPLTDLALFSLGIVGNLLIVIFLSLFIVLDKDKILAYTNRLIPPRYGETATLF
                     QTSVSSSFGGFIRGQAIQGLIYGLIAAGTHLAFGLEFLPASAALVGVLQTIPFFGPFL
                     SWAPPVVVAVLTKPEVAIPTLIVMAVGWFIVMNIVQPRVMATAVGIHPIVVLVSVIIG
                     LKVAGVAGAIFALPFAAVIAAFFHHFLSRNANLPRDVTTRAARRVGEREGRPVRVPKP
                     PALKPDAAAQPEVEPSAVEPSA"
     gene            complement(1080..>2136)
                     /locus_tag="VMZ33_07450"
     CDS             complement(1080..>2136)
                     /locus_tag="VMZ33_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17105.1"
                     /translation="DQALEAAEHADLLPALARGLVAKGGAIGSLGRLREAIALIRAGE
                     ELARAHELTEVLLSALVVGGYYLGEIENAMALDKYREGLELARRTGQRGHMLQFVNNI
                     GYTSFLVGEWDSGLAELDGALAQATDASSRVWLLSNALIIRASRGEAIDDGLAEMDKL
                     VEAHGDPGLVSPTLDTRANLAQATGQTSEARTYWTRMGEKWSSEAAAGYYQGARHGIW
                     SRDLQSLRQDIAALDATGVHGRVVEVRRATLRAAEAALDGQTRDAIARYRDVLASWRD
                     LRVVWEEALAGIDMVTVLDPAEPEVSAAAASTRAILERLRARPYVDRLDTLLARQPAH
                     VGSRVTEAPPRVAAETA"
BASE COUNT          311 a          769 c          668 g          388 t
ORIGIN      
        1 gtcgtggctg ctggcgttca tcctgtcgcc gctcgtcggg ctgatcctga gggcgtttcc
       61 gcggctgccg cgtgtcgtgg tcgtcggcgt tgtctacggc ggtctgttca tcggactgtc
      121 ggcgatcatc ctcgtcgtcg cgggcagcct cgccgcctcg atcggcagct tcatcacgga
      181 cctgcccgaa ttgcaagcga agctccccga gatcctcgcc ggcatccagg gcggattgaa
      241 caatctcggc tttcaggtcg atctagtggc cgccgcccag aacctcttcg cgggtctggg
      301 tgacctgggc aacgacctgg tcagaccact gaccgaccta gccctcttca gcctgggcat
      361 cgtcggcaac ctcttgatcg tcattttcct gtcgctcttc atcgtcctcg acaaggacaa
      421 gatccttgcc tatacgaacc gcctcatccc gccgcgctac ggcgaaacgg cgaccctctt
      481 ccagacgagc gtgtcgtcga gcttcggcgg gttcatccgg ggccaggcaa tccagggctt
      541 gatctacggg ctcatcgccg cggggacgca cctcgcgttc ggcctcgagt tcctaccagc
      601 ctcggccgcg cttgtgggcg tcctgcagac catcccattc ttcgggccgt tcctgtcgtg
      661 ggcgccgccg gttgtcgtgg ccgtgctcac caagccagag gtcgcgatcc cgaccctcat
      721 cgtcatggcc gtcggctggt tcattgtcat gaacatcgtc cagccgcgcg tcatggctac
      781 cgcggtcggc atccacccca tcgtggtcct tgtgtccgtg atcatcgggc tgaaggtcgc
      841 cggtgtcgcc ggcgcgatct tcgccctacc cttcgcggcg gtcatcgcgg cgttcttcca
      901 ccatttcctg agcaggaacg ccaacctgcc gcgagacgtg acgacgcgcg ccgccagacg
      961 cgtcggtgaa cgcgagggcc gacccgtcag ggtgcccaaa cccccagcgc tcaagcccga
     1021 cgcggcggcc cagcctgaag tggaaccgag cgcggtcgaa ccgagcgcct gaggggccgc
     1081 taggcagtct cggccgcgac tctcggcggc gcttcagtca cccgcgaccc gacatgggct
     1141 ggctgccggg ccaacagcgt atcgaggcga tcgacgtagg gcctggcgcg cagacgctcg
     1201 aggatggcgc gcgtcgatgc ggcagctgcg ctcacctccg gctcggcggg atcaagaacg
     1261 gtgaccatgt cgatgccggc cagggcctcc tcccacacca cgcgaagatc tcgccagctg
     1321 gccagaacgt ctctgtagcg cgcgatagcg tcacgtgtct gaccatcgag ggccgcctct
     1381 gccgccctga gggtggcgcg ccggacctcc accacccggc cgtgcacgcc tgtggcgtcg
     1441 agagccgcaa tatcctggcg cagagattgc aggtctctgg accagattcc gtggcgcgca
     1501 ccctggtagt aacccgccgc cgcttcgctg gaccactttt cccccatgcg cgtccagtac
     1561 gttctggctt ccgaggtttg gccggtggcc tgcgccagat tggcacgcgt gtccagcgtt
     1621 ggcgagacaa ggccggggtc gccgtgcgcc tccaccagct tgtccatctc cgccaggcca
     1681 tcgtcgatcg cttcgccgcg cgacgcgcgg atgatcagcg cgttgctgag caaccagacc
     1741 cgcgatgagg cgtccgtggc ctgcgccagc gccccatcca gctcagcgag gcccgaatcc
     1801 cactcgccaa cgaggaagct cgtgtagccg atgttgttga cgaactgaag catgtggcct
     1861 cgttggccgg tccgcctggc gagctcgagg ccctcgcggt acttgtccag cgccatcgcg
     1921 ttctcgatct cgcccaggta atagccgccc acgaccagcg cgctcaacag cacctcggtc
     1981 agctcgtgcg cacgggccag ttcctcgccc gctcgaatca gcgcgatcgc ctcgcgcagc
     2041 cggccgaggc tgccgattgc cccacccttc gcgacgagcc cgcgcgccag tgcgggcaac
     2101 aggtcggcgt gctcagcggc ttcgagggcc tggtcg
//
LOCUS       DATLCO010000324         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_735840, whole genome shotgun sequence.
ACCESSION   DATLCO010000324 DATLCO010000000
VERSION     DATLCO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735840"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..732)
                     /locus_tag="VMZ33_07455"
     CDS             complement(145..732)
                     /locus_tag="VMZ33_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HUR17106.1"
                     /translation="MTGSLLGPLDYMFGETLRVAIAVAVVIAGGVLVAARVRGWTFER
                     VARTWLLLTSLSTIALFTQHNPFGGSGRVLDLNPFGDLRVAADTAGRYRDIVVANVAL
                     FIPLGVALAWRGTRFVKTAAFGAAISILAEAVQYAADHGRVAQSGDVIVNVAGAVVGW
                     ALFVALTGGWGPLSRAYDPPGSELQRRQMAATSRH"
     gene            818..1183
                     /locus_tag="VMZ33_07460"
     CDS             818..1183
                     /locus_tag="VMZ33_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HUR17107.1"
                     /translation="MSDFLGSFLTVAIVGALAVLVVGGMLAIGSLLRPSRPQPQKYLT
                     YESGVDPVGDGWSQSQIRYYIFALLFVMFDVEAVFIFPWAVRLEFLGVFGLVEMLIFI
                     AILALGLVYAWRKGVLRWV"
     gene            1174..1689
                     /locus_tag="VMZ33_07465"
     CDS             1174..1689
                     /locus_tag="VMZ33_07465"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HUR17108.1"
                     /translation="MGLVDKGRAPKALTWLLNYGRKRSLWVYQWGLACCAIEMGAAFG
                     SPRYDVMRLGVIPLPASARQADLVVISGTVTDKMAPPIKRLYEQMPDPKYVISMGSCA
                     NCGGPYWDSYSVTKGVDQIIPVDVYVPGCPPRPEALLEGIVLLQERIQNEDPAQRWRG
                     EPIIVGGGQIS"
     gene            1686..>2068
                     /locus_tag="VMZ33_07470"
     CDS             1686..>2068
                     /locus_tag="VMZ33_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17109.1"
                     /translation="MTDTPTTEEEAPAEAAPVESDPVREAIVEQLAAQFKDAIVDTHI
                     AKGDLWVRVANEQWHDVIAFCKTTLNFRWFDFLSGVDWLINTDLSAEKRWDFGAEKAV
                     PTVDVTSYAGGDTRFQVMCRLKNID"
BASE COUNT          371 a          679 c          670 g          348 t
ORIGIN      
        1 cgcgtgcgaa taactccaac ccgccagcac cgcctcgacc gccgggccaa ccaccgcctc
       61 gtccaacggc ctgccaccac aacgaccacc catacgacac ctcccaatca ggagttgtcg
      121 cgatcaccgt ttgagacagc ctcgtcagtg acgcgatgtg gctgccattt gacgccgttg
      181 aagctcggaa cccggcgggt cgtaggcgcg cgagagtggg ccccagcccc ccgttagagc
      241 gacgaagaga gcccagccca ccaccgcgcc ggccacgttg acgatcacgt cgcccgactg
      301 agccacgcgg ccgtggtcgg ccgcgtactg aaccgcttcg gcgaggattg atatcgcggc
      361 gccgaacgcg gccgtcttga cgaagcgtgt gcctcgccac gccagcgcga caccgagcgg
      421 gatgaacaac gcgacgttgg ccacgacgat gtcgcggtag cgcccggctg tgtcggccgc
      481 gacgcgcagg tcaccgaacg gattgaggtc cagcacccgg cctgagccgc cgaacgggtt
      541 gtgttgcgtg aacagcgcga tggtcgagag gctggtcagc aagagccagg ttcgggccac
      601 gcgctcgaac gtccagcccc gcacccgcgc cgctacgagc acgccgccgg cgataacgac
      661 ggcgaccgcg atagcgacgc gcagcgtctc gccgaacatg tagtcgaggg gaccgagaag
      721 gcttccggtc atgccgcaag ggtaccggcg cgcccctgga aactatttgc aagggatttc
      781 gcaaaacaat gaaagccctc gctaggctcg ccatgcgatg tcggatttcc tgggcagctt
      841 cctaaccgtt gccattgtcg gggccctggc ggtgctcgtt gtgggcggca tgctggccat
      901 tggcagcctt ctgcgcccga gtcgaccgca gccacagaag tacctcacgt atgaatcggg
      961 cgtcgatccg gtgggcgacg ggtggagtca gagccagatt cgctattaca tcttcgcctt
     1021 gctcttcgtc atgttcgacg tcgaagccgt gttcattttt ccgtgggctg tgcgcctcga
     1081 gttcctgggt gtcttcggcc tcgtcgagat gctgatcttc atcgccatcc tggcgctggg
     1141 cctcgtgtac gcatggcgca agggggtcct gcgatgggtt tagtcgacaa gggtcgggcc
     1201 cccaaggccc tcacctggct gctcaactac gggcgcaagc gctcgctgtg ggtgtaccag
     1261 tggggcctgg cgtgttgcgc cattgagatg ggcgcggcgt tcggatcccc gcgttacgac
     1321 gtcatgcgcc tcggggtgat ccccctcccg gcgtcggccc gccaggcaga cctcgtggtg
     1381 atctcaggca cggtgaccga caagatggcg ccgcccatca agcgtctcta cgagcagatg
     1441 cccgacccga agtacgtcat ctccatgggt tcgtgcgcca actgcggcgg cccgtactgg
     1501 gacagctact cggtgacgaa gggcgtcgat cagatcatcc cggttgacgt gtacgtgccg
     1561 ggttgcccgc cgcggcccga agcactgctc gaaggcatcg tgctgttgca agagcgcatc
     1621 caaaacgagg acccggcgca gcgttggcgc ggcgagccca tcatcgtcgg cggcggacaa
     1681 atctcgtgac cgacactccg acgaccgagg aggaggcgcc cgccgaggcg gctccggtcg
     1741 agtcggaccc ggtgcgcgag gcgatcgtcg aacagctggc tgcgcagttc aaggacgcca
     1801 tcgtcgacac gcacatcgca aaaggcgacc tgtgggtgcg cgtcgccaac gagcagtggc
     1861 acgacgtaat cgccttctgt aagacgaccc tcaacttccg ttggttcgat ttcctctccg
     1921 gcgtggactg gctgatcaac acggacctat ccgcggagaa gcgctgggac ttcggcgccg
     1981 agaaagccgt cccgacggtc gacgtgacca gttacgcagg aggcgacacg cgcttccagg
     2041 tgatgtgccg gctgaagaac atcgacag
//
LOCUS       DATLCO010000325         4424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_2229714, whole genome shotgun sequence.
ACCESSION   DATLCO010000325 DATLCO010000000
VERSION     DATLCO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229714"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..998)
                     /locus_tag="VMZ33_07475"
     CDS             complement(204..998)
                     /locus_tag="VMZ33_07475"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HUR17110.1"
                     /translation="MRLVNRWRVLGVLLAAASAIGFGWLVTSHGFDLDQGAVELRGLE
                     YTTPAAVRMAIGLPEDSTPNVFRISTTTMERALNQLPAVDHAEVRAVMPDRLVVALTE
                     HRPIFIVRAAAGGFVIGDSGVVLQSLASNDATTFGLPILDDERVQFAPPLTVGGHLDD
                     VTLGAVVRLAALTPEAIDSEADRLELTVDDTDGYVLTAQPTGWRAVFGHYTPTLRPVD
                     LIDRQVQCLRTRLAAGEADVVVIYLAPLDDACGTYLPRTTRAPLRT"
     gene            complement(1069..2208)
                     /locus_tag="VMZ33_07480"
     CDS             complement(1069..2208)
                     /locus_tag="VMZ33_07480"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HUR17111.1"
                     /translation="MTTQRIRVGVLAGGASSERAISLATGIEIATHLPADRFEVVLID
                     PLALMSRNPRLTEDQRVHARALAAHAGVMEELPERDRRELPAKLQSKILEAAAELQSA
                     TTALVQFEPGVGAGAGRPIDVAFIAIHGQWGEDGTIQGMLELLGIPYVGSGVLASALA
                     MDKVMAKRMLAATGLEVPRGFEVERAHRDPDLEQQLADEIGYPLVAKPVQQGSSVGIN
                     LVDNADELRAALDSALQYDDRVLVEERIFGTELTVGVIGNRPNLEPLPVIEIVTRHEF
                     FDYQAKYDPQLSDEICPARIPDELALRVQDMASRAHTALGCRDLSRTDMIATPDGRVV
                     LLEVNTIPGMTANSLLPKAARTAGIEFGDLCARLIDLALARAQAD"
     gene            complement(2205..3179)
                     /gene="murB"
                     /locus_tag="VMZ33_07485"
     CDS             complement(2205..3179)
                     /gene="murB"
                     /locus_tag="VMZ33_07485"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HUR17112.1"
                     /translation="MTVATDESRTFTDEELARLGTDIQRRIGVKTSRKDPLARFTTMR
                     VGGPADLFAEIHNLFELRAIVRFARSRGLPYFILGRGSDLVISDKGIRGLVIHNRAQG
                     LRFEGTRFTADSGVPMAKAATICRTEGLSGLEFGLAIPGSVGGAVWANAGAHESEVRS
                     VIAQAGVLRADGTEEALDADGLGLAYRESNLKHGPVGAADVVTWASFELEWTDTVEIG
                     RRLDEIRKWRQAHQPLGLPSAGSVFRNPDGNSAGRLIDELGLKGTRIGGAVVSQKHAN
                     FIVNDQNGTAADVRRLGEFVRETVRRETAVELVFEIVFAGDWSGWTTE"
     gene            complement(3176..>4424)
                     /gene="murG"
                     /locus_tag="VMZ33_07490"
     CDS             complement(3176..>4424)
                     /gene="murG"
                     /locus_tag="VMZ33_07490"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HUR17113.1"
                     /translation="EEPSTMRILIAAGGTGGHVYPALAVVRSLRERAPDAEIRWLGGR
                     RGLESTIVPAEGYQYDSLVLRSLRTVDMSIHRVVDPLRLGASVPQATAILARWRPDVI
                     FTTGGYVSVPVALAAAGLRVPLVMWEGNLVPGRSVKATARLARALAVSFAATCQSLKG
                     ACYVTGTPIRSFAGLDKATSRAALGLEAATPVLFVFGGSQAVRKLNRAVAEALPGLIE
                     SATILHMTGERDYADALKRRKELPEERRVRYRPFAFLRAEMADALVAADLLVGRAGSS
                     TLAEASALGLPMVVVPYPHASAHQEANARAMADAGAARIVPDEQFDAAALMEAAALLN
                     DAVTLDGMRAAARRFGRPGAAAAVAELCLALAEGAKLPSAAAVERMSRATQATTEPQR
                     DARARSSGAQPAPAGAPAKKTQS"
BASE COUNT          707 a         1519 c         1508 g          690 t
ORIGIN      
        1 acgaatccat ctggcccttg ccgatgacag tgacgccttc ctcgcgcgtc acctcgccga
       61 tgagcgcaac gaccttgctg gtgccaacgt cgagcgcgac gagtacggcc tctctatcca
      121 cgggccgatt gtcgcctaaa ggccgcaagg gagcggcgcc tgttcagcgc aaggtggagc
      181 agccttgacc gcagcaggtg caatcaagtt cgcagtggtg cccgagtcgt gcgcggcagg
      241 tacgtgccac aggcgtcgtc gagcggcgcg aggtagatca cgaccacgtc agcctcgccg
      301 gcggccagcc gcgtgcgcag gcactgcacc tggcggtcga tcaggtctac cggtcgcagc
      361 gtcggcgtgt aatggccaaa cacggcacgc cagcctgtgg gctgggccgt gagcacgtaa
      421 ccgtccgtgt cgtcaacggt gagctccagc cgatccgcct ccgaatcgat cgcctccggc
      481 gtcagtgcgg ccagacgcac caccgcgcca agcgtgacgt cgtccagatg cccacctacc
      541 gtgagcggcg gcgcgaactg gacgcgctcg tcgtcaagga tcggcaggcc gaacgtcgtg
      601 gcatcgttgg acgccagcga ctgcaggacc acgccgctgt cgccgatcac gaagccaccg
      661 gcagccgccc gaacgatgaa gatcggacgg tgctcggtca gggctacaac caggcggtcg
      721 ggcatgacgg cgcgaacctc ggcatggtca accgcaggta gctggttgag cgcccgctcc
      781 atggttgtcg tcgagattcg gaagacgttg ggcgtcgagt cctccgggag cccgatcgcc
      841 attcggaccg cggctggtgt ggtgtactcg agcccgcgga gctcgacggc gccctggtcc
      901 aggtcgaacc catgcgacgt cacgagccag ccgaacccga ttgcggacgc tgctgccagc
      961 agcacaccca gcacacgcca gcggttgacc agccgcacgg ccccagcctg gccgcgtgac
     1021 cgtggcggcc gccgattgcc gcctccacga cggttctgcg ggcgagcgct aatcggcctg
     1081 ggcccgtgcc agagctagat cgatcagccg ggcgcagaga tcgccgaact caatgcccgc
     1141 cgtccgcgcg gcttttggca agagactgtt ggcggtcatg cccgggatcg tgttcacctc
     1201 cagcaggacg actcggccgt caggcgtggc gatcatgtcg gtgcgcgaca gatcgcggca
     1261 acccaaggcc gtgtgcgcgc gggacgccat gtcctggacg cggagagcca gctcgtcggg
     1321 gatgcgcgcg ggacagatct cgtcggagag ctgcgggtcg tacttggcct ggtagtcgaa
     1381 gaactcgtgg cgcgtgacga tctcgatgac cggcagcggc tcaaggttcg gccgattgcc
     1441 gatcacgccg accgtcagct ctgtcccgaa tattcgctcc tcgaccagga cgcggtcgtc
     1501 gtactggagc gctgagtcga gggccgcgcg tagctcgtcg gcgttgtcca ccaggttgat
     1561 gccgacgctg gacccttgct ggacgggctt cgcgacaagg ggatagccga tctcgtctgc
     1621 cagctgctgc tcgaggtccg gatcacgatg ggcgcgctca acctcgaagc cacgcggcac
     1681 ttccaggcca gtcgcggcga gcatgcgctt ggccatgacc ttgtccattg ccaacgccga
     1741 ggcaagcacg cccgaaccaa catatggaat gcccagcagc tcgagcatgc cctggatcgt
     1801 cccgtcctcg ccccactggc catggatcgc gatgaaggcg acgtcgattg gccggcctgc
     1861 cccagcgcca acgccgggct cgaattggac gagcgccgtg gtcgctgact gcagctcggc
     1921 ggcggcctcg aggatcttgg attggagctt cgccggcagc tcacgtcggt cgcgctcggg
     1981 cagctcctcc atgaccccgg cgtgcgcggc gagcgcgcgc gcgtggacgc gctgatcttc
     2041 ggtgagacgc gggttgcggc tcatcagcgc gagcggatcg atgaggacga cttcgaatcg
     2101 gtcggccggc agatgcgtcg cgatctcgat gcctgttgcc agggagatgg cacgctccga
     2161 cgacgcgccc ccggccaaaa ccccgacgcg gatgcgctgc gttgtcactc cgtcgtccag
     2221 cccgaccagt ctccggcaaa cacgatctcg aagacgagct cgactgctgt ttcgcgacgg
     2281 accgtctcgc gcacgaactc gcccagtcgc cgtacgtcgg cggcggtgcc gttctggtcg
     2341 ttgacgatga agttggcgtg cttctggctc acgaccgccc cgccaatgcg cgtccccttc
     2401 agccccagct cgtcgatgag gcggccggcg gagttcccgt cgggattgcg gaacacgctg
     2461 ccagccgacg ggagtccaag tggttggtgt gcctggcgcc actttcggat ctcgtccagc
     2521 cggcgcccga tttcgacggt gtcggtccac tcgagctcga agctcgccca cgtcacaacg
     2581 tcggcggcgc ccaccgggcc gtgcttgagg ttgctctcgc ggtaggccaa cccgagtccg
     2641 tcggcatcga gcgcctcctc ggttccgtcg gcacgcagca ctcctgcctg ggcgataacg
     2701 gagcgcactt cggactcgtg cgcccccgca ttcgcccaga cggcaccgcc gaccgacccc
     2761 ggaatggcga gcccgaactc cagccccgac agaccctcgg tgcggcagat ggttgctgct
     2821 ttggccatgg ggacgcccga gtcggcggtg aagcgtgtgc cttcgaagcg cagcccctgc
     2881 gcgcggttgt ggatgaccag gccgcgaata cctttgtcgc tgattacaag gtcggagcca
     2941 cggccgagaa tgaaatacgg cagcccgcga gagcgagcga agcggacgat ggcgcgtaac
     3001 tcgaagaggt tgtgaatctc ggcgaagagg tcggccgggc cgccgacgcg catcgtggtg
     3061 aagcgtgcca gcggatcctt gcgcgacgtc ttgacgccga tacggcgttg gatgtcagtg
     3121 cccagccggg cgagctcctc gtcggtaaac gtccgcgact cgtcggtggc caccgtcacg
     3181 actgagtctt cttcgccggg gcgccggcgg gcgccggctg cgcgccactt gaccgagctc
     3241 gagcgtcgcg ctggggttcg gtcgtggctt gcgtggctcg gctcatgcgc tccacggcag
     3301 ccgcgctggg cagcttcgca ccctcggcca gcgccaggca cagctcagcg acggcggccg
     3361 ccgctccggg tcgtccgaaa cgtcgcgccg ccgcgcgcat cccgtcaagc gtcaccgcat
     3421 cgttgagcag cgcggccgct tccatcagcg ctgccgcgtc gaactgctcg tcggggacta
     3481 tgcgggcggc gccggcatcg gccatcgcgc gcgcgttcgc ctcctggtga gcagacgcgt
     3541 gcgggtacgg gacaacgacc atgggcaggc caagggcgct cgcctctgcc agcgtcgatg
     3601 atcccgcgcg ccccaccagc aggtcggcgg cgacgagcgc atcggccatt tccgcccgta
     3661 ggaaggcgaa tgggcgatag cggacgcgcc gctcttccgg gagctccttg cgtcgcttga
     3721 gggcgtcggc gtagtcgcgc tcgccggtca tatgcaggat cgtggcgctc tcgatgagcc
     3781 ctggaagcgc ctccgccacg gcgcggttga gcttgcgcac ggcctgcgag ccgccgaaga
     3841 cgaacagcac cggcgttgcg gcctcgagcc cgagggcagc gcgtgacgtg gccttgtcga
     3901 ggccggcaaa cgagcggatc ggcgtgccgg tgacgtagca cgcgcccttg agcgactggc
     3961 acgtcgcggc aaatgacacg gccaacgctc gtgcgaggcg cgccgtcgcc ttcacgctgc
     4021 gtcccggaac gaggttgccc tcccacatga cgagcggcac acgcaaacca gcggcggcca
     4081 gcgccaccgg caccgacaca tagccccccg tagtgaagat cacgtcgggc cgccagcggg
     4141 cgaggatggc tgtggcctgt gggacggagg cgccgaggcg tagcgggtcg accacccggt
     4201 ggatggacat gtccaccgtc cgcaaggatc ggagcacgag gctgtcgtat tggtagccct
     4261 ctgccgggac gatggtcgac tccagtccac gtcgaccccc cagccaccgg atctcggcgt
     4321 caggagcccg ctcgcgcagt gaccgaacga ccgccagcgc cgggtacacg tgcccgcctg
     4381 tgccaccggc cgcgatcaga atccgcatcg ttgaaggttc ctcg
//
LOCUS       DATLCO010000326         5972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6335895, whole genome shotgun sequence.
ACCESSION   DATLCO010000326 DATLCO010000000
VERSION     DATLCO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6335895"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VMZ33_07495"
     CDS             <1..276
                     /locus_tag="VMZ33_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17114.1"
                     /translation="NTAAALERQVRAYQPWPGSFVETHAGRLVVWRAHIAPTPPTDGL
                     TLSAADGALVLDEVQLAGGKRMTAEDLLRGRPDLQIVPSPATTAASG"
     gene            286..1497
                     /gene="rlmN"
                     /locus_tag="VMZ33_07500"
     CDS             286..1497
                     /gene="rlmN"
                     /locus_tag="VMZ33_07500"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HUR17115.1"
                     /translation="MPVTVGLAPTTPALIQLPPAGARDPRPGLSGAPVDAISSWLSQQ
                     RLPAYRARQLADHLWSGAAQSPSELHTLPSAIRDRLAADFRITTLGDTEIKPADNELT
                     QKALHRLDDGRLIESVLMRYPARGWRRARATVCISSQAGCAVGCPFCATGELGFERDL
                     ETAEIVDQARFWRHWLAGVGQRLTNVVFMGMGEPLLNADRVLEAAAALTDPGRFGLGA
                     RHVTISTSGVVPGMQRLIAERPQYTLAVSLHAARNELRDVLVPLNRRYPVGEVVDAAA
                     RYSAVTGRRVTYEYVMIDSINDTPEDGTALAKLLAGHLAHVNLIPMNPVAHTPWQGSS
                     LERSEAFAAKLRSAGISTTVRRNRGVEIGAACGQLAADRAGAPAPSAVARRRELLVVQ
                     SASALSVRART"
     gene            1521..2204
                     /gene="rpe"
                     /locus_tag="VMZ33_07505"
     CDS             1521..2204
                     /gene="rpe"
                     /locus_tag="VMZ33_07505"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HUR17116.1"
                     /translation="MVEVAASILTADFGNLYRVVRKLERAGVDRLHLDVMDGHFVPNL
                     TFGPNVVAAFRRLTRLPLDVHLMISEPSRYVDRFLDAGADSITFHVEVDESEESKRAT
                     LEQIATARRQPGLAISPGTPVEAVRPFADQLGILMVMTVVPGWGGQRFMADEASKIAD
                     AREMLDGRPGTAVHVDGGVSAETAAVVGWHGVDVCVVGSALFQRGHDASLEVDAVREN
                     ARAGARGRV"
     gene            2204..2650
                     /gene="dtd"
                     /locus_tag="VMZ33_07510"
     CDS             2204..2650
                     /gene="dtd"
                     /locus_tag="VMZ33_07510"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HUR17117.1"
                     /translation="MRVLLQRVERADVRVDRKLVGAIGLGLVAFVGVGREDTEAEVDQ
                     LASKAVELRIFRDEDGKTNLSLVDVGGEMLAISQFTLYADTRKGRRPSFIYAGEPALA
                     ERLYERFAATVEGRGVTVARGVFGSEMKVELINDGPMTIWLDSAAK"
     gene            complement(2662..2847)
                     /locus_tag="VMZ33_07515"
     CDS             complement(2662..2847)
                     /locus_tag="VMZ33_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HUR17118.1"
                     /translation="MCAICGKTSMGGFNPQSVGMNRVRAHRRMEPNLQPMRLGQGPAA
                     KRVLVCTRCIRTLQKTA"
     gene            2970..4652
                     /locus_tag="VMZ33_07520"
     CDS             2970..4652
                     /locus_tag="VMZ33_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HUR17119.1"
                     /translation="MIDGAWEGREFLAALRHASALLQSRVEEINALNVFPVPDGDTGS
                     NMLATILAALEEADELPLDERTVSRVGAAISFGALMGARGNSGVILSQLFRGIGEAVE
                     GSNTIGGSELAEALTMGSRAAFASVSRPVEGTILTVARDAAEAASELARHRAPLEEVL
                     AGAVAAAHASVAKTPDLLPVLRQAGVVDAGGRGLELLLQGALSSLRGETAPELPVLPL
                     EIVLPLTDALEAEGFGYETVFVVTPEKGRSLNLPEIRNELEKLGVSVLVAGDQRAIKV
                     HVHTEHPDQILAFGLTLGSLSRIQVENIDRQAMVHRDRLESHEAGMGTPAPDKPSSAP
                     FSGAPTPSASPAVVAVAPGAGLARILMTLGATIVDGGPSANPSAGDIADAVRRTGATE
                     VVILPNNPNVRLAAQQAGTLVSHARVTVLPTRNAAEGIAAMLAYDADAGLDSLAREMT
                     ASAGRIQTLQVTAAVRDASIGRHKVRRHDYMVLGPDDGLVAVDADRTRAVLAAIKKLQ
                     IGFELLTIYRGHEINVLTADALRGAIAGEWPGLEIELVDGGQPHYDLLIAAE"
     gene            4663..>5972
                     /locus_tag="VMZ33_07525"
     CDS             4663..>5972
                     /locus_tag="VMZ33_07525"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUR17120.1"
                     /translation="MTAPSVDPHAADEGAATILDASLLDLQIVPKRVANILGSRLALH
                     TVRDLLFHLPRQHDDFSRQMTLRQLREKAVEGPVSATVTVNDLRVEQGFRRRIQRTVA
                     RISDDTGEGEAIWFGRRYIERRLKPGEEVLLSGKVELRGWVPRFQNPEFGAATPDALH
                     AGRIVPVYRLTAGVTGAYLRQRIRTVLDMALPAYAEYLPRQVRSLAGSDAPPISDAIE
                     WMHFPQDFGKLERALQRLAFDELLALQIGMVARQRQRRSERTDPIDVSDDHAKRAVSG
                     VEQVISRQVSARRGVTEQINLTDDQRKAVADIRSDLRSGRPMMRLIQGDVGSGKTAVA
                     ALALAAVADVGSQGALLAPTDLLARQHHVTLSALLEPLGHGVTLLTGSLGVAQRREAL
                     QLLGAPVAMATSGRSLGRVVVGTHALVQDAVAFEDLRLVVVDEQ"
BASE COUNT          956 a         1991 c         2018 g         1007 t
ORIGIN      
        1 aataccgcgg ccgcgctcga gcgccaggtg cgtgcctacc aaccgtggcc gggcagcttc
       61 gtcgagaccc atgccggccg gctagtcgtg tggcgcgccc acatcgcgcc aacgccgccc
      121 acggacggac tcacgctgtc agccgcagac ggcgcccttg tgctcgacga ggtccagctc
      181 gccggcggta agcggatgac cgccgaggat ctgctgcgcg gacgccccga ccttcagatc
      241 gtgccgtcgc cagccactac cgcagcttcg ggctagcatc gtcgcatgcc cgttaccgtc
      301 ggcttggcgc cgacgactcc agccctgatc cagctacctc ccgccggtgc gcgcgaccct
      361 cgtcccggcc tgtcgggcgc acctgtggac gccatcagct cgtggctgtc ccaacagcgc
      421 ctgcctgctt atcgggcgcg gcagctcgct gaccacctgt ggtcgggtgc cgcccagtcg
      481 ccgtcggagc tgcacaccct tccgtctgcg ataagagatc gattggcagc cgacttccgc
      541 atcacaaccc tgggcgacac ggagatcaag ccggccgaca acgagctcac acagaaggcg
      601 ctccaccggc tggacgacgg ccgacttatc gagtcggtgc tcatgcgcta tccggctcgg
      661 ggctggcggc gcgcccgggc caccgtgtgc atctcgagtc aggctggctg cgctgtcggc
      721 tgcccgttct gcgctaccgg tgagctgggc ttcgaacgtg acctggagac agccgagatc
      781 gtcgaccagg cacggttctg gcggcactgg ctcgcgggcg tcggccagcg actcaccaac
      841 gtcgtgttca tgggcatggg cgaaccgttg ctcaacgccg accgcgtgtt ggaagcggct
      901 gcggcgctca cggatccggg ccgcttcggg ctgggagctc gccacgtcac gatcagcacc
      961 agcggagtcg tgccggggat gcagcggctc atcgccgaga ggccgcagta caccctcgcg
     1021 gtgtcgttgc atgccgctcg caacgagctt cgcgacgtgc tcgtgccgct caaccgcagg
     1081 taccccgtcg gggaggtggt cgatgctgcc gctcgctact cagcggtgac ggggcgacgc
     1141 gtcacctacg agtacgtgat gatcgactcc atcaatgaca cgcctgagga tgggaccgcg
     1201 ctcgcgaagc tcctcgccgg acatcttgcg cacgtgaacc tcatcccgat gaacccggtc
     1261 gcgcacacgc cctggcaagg aagctcgctc gaacgctccg aggccttcgc cgcaaagctc
     1321 cgcagcgcag gtatcagcac gaccgtgcgg cgcaaccgtg gagtcgagat cggtgctgcc
     1381 tgcggtcagc tcgcggctga tcgcgccggc gcaccagcac cgtcggccgt tgcgcgccgg
     1441 cgtgagctgc tcgtcgtcca gagcgcgtcc gcgctctcag tgcgcgcccg cacctagcaa
     1501 tccggcaccg agcaggacct gtggtcgagg tcgcagccag catcctcact gcggacttcg
     1561 gcaacctgta tcgagtcgtc cgcaagctcg agagggcggg cgtggatcgc ctgcacctcg
     1621 atgtgatgga cggccacttc gtcccgaacc tgaccttcgg accgaacgtt gtcgccgctt
     1681 ttcggcgtct cactcgtctg ccactcgacg ttcacctgat gatcagcgag ccgtcgcgct
     1741 acgtggaccg gttcctcgac gccggcgcgg actcgatcac attccacgta gaggtcgacg
     1801 agtccgagga atccaagcgc gccacgcttg agcagatcgc gactgctcga cggcagccgg
     1861 gtctggcgat cagcccgggc acaccggtgg aagcggtccg ccctttcgcc gatcaactgg
     1921 ggatcttgat ggtgatgacg gtggtgccgg gctggggcgg ccagcgcttc atggccgacg
     1981 aggcgtcgaa gattgccgac gccagggaga tgctcgacgg gcggccggga actgcggtcc
     2041 atgtcgatgg cggggtcagc gccgagaccg cggcggtcgt cgggtggcac ggcgtggacg
     2101 tgtgcgtcgt cggctcggcc ctgttccagc gcggtcacga cgcttccctg gaggttgacg
     2161 ccgtccgcga gaatgcccgc gccggtgcgc ggggacgagt ctagtgcggg ttctgctcca
     2221 gcgggttgag cgcgccgacg tgcgcgtcga tcgcaagctc gtcggtgcga tcggcctggg
     2281 gttggtcgcc ttcgtcggtg tcggtcggga ggacacggag gcggaggttg atcagctggc
     2341 ctccaaggcc gtcgagctgc gcatctttcg cgacgaggat ggcaagacca atttgtccct
     2401 tgtcgacgtc ggcggcgaga tgctggcaat cagccagttc acgttgtacg cggacacgcg
     2461 caagggccgt cggccgtcgt tcatctacgc cggagagccg gcactggcgg agcggctcta
     2521 cgaaaggttc gcggcgacgg ttgaaggccg gggcgtgact gtcgcacgcg gggtgttcgg
     2581 atcggagatg aaggtcgagc tcatcaacga cgggccgatg acgatttggc tcgactcagc
     2641 ggcgaagtag atccgaacga tttaggcggt cttctggagc gtgcggatgc agcgagtgca
     2701 gacgagcacg cgcttggcgg cagggccctg accgaggcgc atcggctgca ggttgggctc
     2761 catgcgacgg tgagcgcgca ctcggttcat accgacggat tgcgggttga acccacccat
     2821 cgaggtcttg ccgcagatcg cgcacattcg ggccatggtt tgacgcaact ccgttgattt
     2881 cgtagaggaa acgacgacgc cgtcagcgtc cgtcggctgg ctactataca tgagcccgtg
     2941 gcgcgaccga tctgtgggag acgcttgact tgatcgacgg agcctgggag ggccgcgagt
     3001 ttctggccgc gttacgccat gcctccgcgc tgctccaatc acgcgtcgag gagatcaacg
     3061 ccctgaacgt ttttcctgtt cctgatggcg ataccggctc gaacatgctc gccacgatcc
     3121 tggccgcgct tgaggaggcc gacgagctgc cgctcgacga gcggaccgtg agtcgtgtcg
     3181 gcgcagcgat cagcttcggt gccttgatgg gcgcgcgagg caacagcggc gtgatcctgt
     3241 cgcagctgtt tcgcggcatc ggcgaggcgg tcgagggcag caacacgatc ggcggctccg
     3301 agctcgccga ggctctgacc atgggttcgc gagcggcatt tgcgtcagtg tcccggcccg
     3361 tggaaggcac catcctgacg gtcgcgcggg acgccgccga ggccgccagc gaactggccc
     3421 gccatcgcgc gccgttggag gaggtccttg ccggggccgt ggcggctgcc cacgcatctg
     3481 tcgcaaagac gccagacctg ctccccgtcc tgcgccaggc cggcgtagtt gacgccggtg
     3541 gccgcggctt ggagcttctc ctgcagggag ccctgtcgtc gttgcgtggc gaaaccgcgc
     3601 ccgagctgcc cgtcctgccc cttgagatag tccttccgct cacggacgcg ctcgaggccg
     3661 agggcttcgg ctacgagact gttttcgtcg taactcctga gaaaggtcgc agtctcaatc
     3721 tcccggagat ccggaacgag ctcgagaaat taggagtgtc ggtactggtc gcgggtgacc
     3781 agcgtgcgat caaggtgcac gttcatacgg agcatccgga ccagatcctg gcttttggcc
     3841 tgactctcgg atctctgagc cgcatccagg tcgagaacat cgatcgacag gcgatggtcc
     3901 atcgcgaccg gctggagtcg cacgaagctg gtatgggcac gccagcgccc gacaagccct
     3961 ctagcgctcc gttttccggc gccccaaccc cgagtgccag tccagctgtc gtcgccgtcg
     4021 cgccgggggc cggattggcg cgcatcctaa tgacactcgg cgcgacgatc gtcgatggcg
     4081 gcccgagcgc gaacccgtct gcgggcgata tcgcagacgc cgtccgccgg accggcgcga
     4141 cggaggtcgt catcctgccc aacaacccca acgtgcgact cgcggcccaa caggcgggca
     4201 ccctcgtgtc gcatgcccgg gtgaccgtcc ttccgacccg caacgctgct gagggcatcg
     4261 ccgccatgct cgcctacgac gcggatgccg gcctcgattc cctcgctcgc gagatgacgg
     4321 catccgctgg tcggatccag acactgcagg tgacggcggc cgtacgcgac gcgagcatcg
     4381 gccggcacaa ggtgcgtcgc cacgactaca tggtgctcgg ccccgacgat ggactggtcg
     4441 cagtggacgc tgaccggaca cgcgccgtcc tcgccgcaat aaaaaaattg cagatcggct
     4501 tcgagctgtt gacgatctac cgcggccacg agatcaacgt gttgacggcc gacgcgttac
     4561 gtggcgccat cgctggggag tggcccggtt tagagatcga gctcgtcgac ggcggccagc
     4621 ctcactacga cctgctgatc gccgccgagt gacacgagcg gagtgacggc gccgagcgtc
     4681 gacccacacg ccgcggacga gggcgccgcc acgatcctcg acgcgtcgct acttgacctg
     4741 cagatcgtcc cgaagcgtgt cgcgaacatc ctgggctcac gtctggcgct gcacacggtt
     4801 cgcgacctgc tattccacct gccacgccaa cacgatgact tcagccgtca gatgaccctg
     4861 cgccagctgc gtgagaaggc cgtcgaggga ccggtatcgg cgacagtcac agtcaacgac
     4921 ctgcgcgtcg agcagggctt tcggcgtcgc attcaacgca ctgtggcgcg gatcagcgac
     4981 gacacggggg agggggaggc gatctggttc ggccgccgct acatcgaacg gcgcctcaag
     5041 ccgggcgagg aggtccttct ttcgggcaag gtcgagctgc gcggctgggt cccccgtttc
     5101 cagaacccag agttcggtgc ggcaacccca gatgcgctgc acgccggccg aatcgtgcct
     5161 gtttaccgcc tcacggctgg cgtgactggc gcctacctgc gccaacggat ccgaacggtc
     5221 ctcgacatgg ccctccccgc ctacgccgag tacctgccgc gccaggtcag atcgttggcg
     5281 ggctcggacg cgccgccgat cagcgatgcc atcgagtgga tgcactttcc acaagatttc
     5341 ggcaagctcg agcgggcttt acagcggctg gcgttcgatg agctgctggc tctccagatc
     5401 ggcatggtcg ctcgtcagcg ccagcgaagg agcgaacgga ctgatccgat agacgtgtcc
     5461 gatgaccacg ccaagcgggc ggtctctggc gtcgagcagg tcatcagccg ccaggtcagc
     5521 gcacgccgcg gggtgacaga gcaaatcaac ctgacggacg accagcgcaa agccgtcgcc
     5581 gacattcgat cggacctgcg ctcagggcgg ccaatgatgc ggctcatcca gggcgacgtt
     5641 ggctccggta agactgcggt agctgcgctc gcgctggccg cggtcgccga tgtcggtagc
     5701 cagggcgcgc tcctcgcgcc gacggacctt ctggcgcggc agcatcacgt cacgctctcg
     5761 gcccttctgg agccgctcgg ccatggagtg acgctcctca ccggctcgtt gggtgttgcg
     5821 cagcgccgcg aggcgctcca gctgctgggt gcgccagttg cgatggcaac cagcggccgg
     5881 agcctcggac gggttgtggt gggcacgcat gcgctcgtcc aggacgcggt agcgttcgag
     5941 gacctgcgtc ttgtcgttgt cgatgagcag ca
//
LOCUS       DATLCO010000327         2994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8595836, whole genome shotgun sequence.
ACCESSION   DATLCO010000327 DATLCO010000000
VERSION     DATLCO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8595836"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VMZ33_07530"
     CDS             <1..508
                     /locus_tag="VMZ33_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HUR17121.1"
                     /translation="FRRELFVFKRALLAAFAFAVCVVAVQVGPTQAAASYSEATAVKQ
                     FAARQINKPFRLGANGMTKFDCSGLVYRTYLENGLLRRVGGNQRAAGYYRWFRDRGLL
                     TNNPKSGDLVVWGRRGHPVEHIGIFVGYNKNGSPMAISALTTGVARHRVNGISMPVRA
                     YLSVNLNR"
     gene            626..1399
                     /locus_tag="VMZ33_07535"
     CDS             626..1399
                     /locus_tag="VMZ33_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HUR17122.1"
                     /translation="MNIVVLLKAVPVVGTERLGNDLLTDRTGQSEANGNDEYVLERAL
                     QLTEAHGGEVTTLTLGPATAVEAVRKALAMGAARAVHVLDDGVRGSDMRATVAILEAA
                     LRKLEYDLVFAGADTSDAQGGLVGAALAARLGLPYLSYAADIQPSGAGVRIHRLHATG
                     YDVLESPTPAFVMGTQLLGAPRYPSLRGIMQARSKPIETWSLADLGIDGGTVGSAAAT
                     TKTVDASKPGERAGATFVREPADVAVAQVVDFLSQRRLI"
     gene            1399..2376
                     /locus_tag="VMZ33_07540"
     CDS             1399..2376
                     /locus_tag="VMZ33_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HUR17123.1"
                     /translation="MATIWALGEISDGVPSKLTLELATLARHLAEKSGGESKTVLIGK
                     DASSAANEVAPYAGGVIAVDTEVAGPVAATVASRAAALIAERNPDFVLVGASSDGKDV
                     AGLLLGLTDMPVLANAAAVEWADGGPQVEMSTYGGKLVTRSAFTGGRGIIVVRAATAT
                     AEKATSPGAVETISVSVASELPQVTVGERVEEAGAAAPIEEARIIVGGGRGVGSAEGF
                     KLVQDLADALGGAVGATRAVVDSGWISYSQQIGQTGKIVKPALYVAAGISGAIQHKVG
                     VQTAGTIVAINRDPDAPIAEFADLFVVGDLTEILPKLTEAIRARKSGAG"
     gene            complement(2510..>2994)
                     /locus_tag="VMZ33_07545"
     CDS             complement(2510..>2994)
                     /locus_tag="VMZ33_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17124.1"
                     /translation="FAPLPLSALGTKRVFQRPSSTMPGTGGVDVGDGDALGVAVGVAV
                     GLGVGVAVGVAVGVAVGVAVGVAVGVGLAVAVGTWVGVAGGCVVAGVDGGSVGGSVGV
                     GVTTATAVAVGMTVGVGVPSSGGGVGALSEPQPTMTRTSSRAAHSRRRVIYVPSNRQP
                     "
BASE COUNT          522 a          988 c          985 g          499 t
ORIGIN      
        1 atttcgaagg gaactattcg tgttcaagcg cgctcttttg gccgcattcg cttttgccgt
       61 gtgcgttgtg gccgtccagg tcgggccgac ccaggccgcg gcctcatatt ccgaagctac
      121 tgctgtcaag caattcgccg caaggcagat caacaagcca ttcaggctcg gagccaatgg
      181 catgaccaag ttcgactgtt cagggctcgt ctatcggacc tatcttgaga atgggctctt
      241 gcgccgcgtc ggcggcaatc agcgagccgc gggctactac cgctggttcc gggaccgcgg
      301 gctgctcacc aacaacccca agtcaggcga cctcgtcgtc tgggggcgcc gcggccatcc
      361 ggtcgagcac atcggcatct tcgttggcta caacaagaat ggttcaccca tggcgatcag
      421 cgctctcaca acgggcgtgg ctcgccaccg agtcaacggc atcagcatgc ccgttcgggc
      481 gtacctcagc gtaaatctca accgttgacg ggctgaccct cgtagtccgt cacgcggttg
      541 tctcctgggc ggggctgcgg ccgtgcagcc ccgcccgatt tccccaaacg ccgttagcct
      601 tcccgctgca caggggaggt agagagtgaa tatcgtcgta ctgctgaagg ccgtgcctgt
      661 cgtgggcacc gagcgcttgg gcaacgacct gctaaccgat cgcacaggcc agtctgaagc
      721 gaacggcaat gacgaatacg tgctcgaacg agcactccag ctgaccgagg cccacggtgg
      781 cgaggtgacg acgctcacgc tcggacccgc gacggcggtt gaggccgtgc gcaaggccct
      841 ggcaatgggt gcggcacgag ccgtccatgt tctcgacgac ggagtgcgcg gttccgatat
      901 gcgtgcgacg gtcgccatcc ttgaggcggc actccgcaag ctcgagtacg acctggtctt
      961 cgcgggtgcc gatacgtcgg atgcccaggg tggcctggtg ggcgctgcct tggcggcccg
     1021 gctcgggctg ccctatctct cgtatgcggc agacattcag ccgagtgggg ccggagtgcg
     1081 catccatcgt ctccacgcga ccggctacga cgtcctcgag tcgcccaccc cggcattcgt
     1141 aatgggcacg cagctgctcg gtgcgccgcg ctacccgtcg ctgcgcggga tcatgcaggc
     1201 gcgctcaaag ccgatcgaga cctggtcgtt ggccgacctg ggcatagacg gtggaacggt
     1261 tggtagcgcc gccgccacga ccaaaaccgt ggatgccagc aagcccggag agcgtgccgg
     1321 cgcgacgttt gtccgtgagc cggctgacgt tgccgtcgct caggttgtcg acttcctctc
     1381 gcagcggagg ctcatctgat ggccacgatc tgggccctcg gtgagatcag cgacggcgtg
     1441 ccaagcaagc tgacactcga gcttgcgacg ctggcccgac acttagcgga gaagtcgggt
     1501 ggcgaaagca agaccgtcct catcggcaag gacgccagca gcgcggccaa cgaagttgcc
     1561 ccatatgcgg gcggcgttat cgcggttgac acggaagtcg ccggtcccgt ggcggcgacc
     1621 gtggcgtcgc gtgcggccgc cctgatcgcg gagcgcaacc cggactttgt acttgtcggc
     1681 gcctcgagcg acggcaagga tgtggctgga ctgttgctgg gcctgacgga catgccggta
     1741 ctcgccaacg ccgcggccgt cgaatgggct gatggcgggc cacaggtgga gatgagcacg
     1801 tatggcggca agctggtgac gcgcagcgcc ttcaccggcg gccgcggcat catcgtcgtc
     1861 cgcgccgcga cggcgacagc tgagaaggcc acatcaccgg gcgccgtcga aacaatctct
     1921 gtgagcgtgg ccagtgagct gccacaggtg acggtcgggg agcgcgtcga ggaggccggc
     1981 gcagccgcgc ccatcgagga agcgcggatc atcgtcggtg gtggccgcgg cgttggtagc
     2041 gctgagggat tcaagctcgt ccaggatttg gccgatgcgc tcggtggcgc cgttggtgcc
     2101 acgcgcgccg tggttgattc gggctggatc agctacagcc agcagatcgg ccagacgggc
     2161 aagatcgtga agcccgccct ctacgtggct gcggggatca gcggcgcgat ccagcacaag
     2221 gtcggtgtgc agacggccgg aacgatcgtc gccatcaatc gcgaccccga tgcgccgatc
     2281 gctgagttcg cggacctttt cgtcgtcggc gacctcacgg agatcctgcc aaagctgacc
     2341 gaggccatcc gcgcccgcaa atcaggtgcc ggctagggat tgaaaatggg cacccgcaga
     2401 actttctgtg ggtttcgcgc gtcatagcca ggtagagcgg ccgaatcgcg acgtttgtcg
     2461 cgtgtcggca ctccgttggc gcgctaggct gttcgcgtca ggttcagggt cagggctgac
     2521 gattcgaggg gacatagatg acacgcctgc gcgagtgcgc ggccctacta ctcgtcctgg
     2581 tcattgtggg ttgcggttct gatagtgcgc caaccccgcc gccggacgat gggacgccaa
     2641 cgccaacagt cataccgact gcgacagccg tagccgtcgt cacaccgacg ccgacagatc
     2701 cgccgactga cccgccatcc accccggcga cgacgcaacc cccggcgacg cccacccagg
     2761 tgccgactgc aacagccagt ccgactccga ccgccacacc gacggcaacg ccgaccgcaa
     2821 cgcccacggc cactccgact gccacaccga cgccaaggcc cacggcgact cccaccgcta
     2881 cgccgagcgc gtcgccgtca cctacgtcca cgccacccgt tccgggcatg gtgctgctcg
     2941 gccgctggaa aacgcgcttt gttccgagcg cgctcaacgg caatggagcg aaca
//
LOCUS       DATLCO010000328         4517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6348531, whole genome shotgun sequence.
ACCESSION   DATLCO010000328 DATLCO010000000
VERSION     DATLCO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6348531"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VMZ33_07550"
     CDS             <1..576
                     /locus_tag="VMZ33_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HUR17125.1"
                     /translation="EPEAIAVEGVGETCPECNEGTLVAKRGRFGPFAGCSRYPDCKFI
                     KKSGPPPPDPLPFDVVCPKCNEGQLTARRARRTGSVFWGCSRYPKCDFTSSREPLGAF
                     HETDDGPVAREGEAGAMCLKCGAAIEATGEIVPGSRLAGGEPNPEALAPPRRGRGGSG
                     RSATGRAAGSRRPSAGGGAARTNGRRARPAA"
     gene            573..1508
                     /locus_tag="VMZ33_07555"
     CDS             573..1508
                     /locus_tag="VMZ33_07555"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HUR17126.1"
                     /translation="MTGADALDSFLERLAARDSSEHTRRAYRTAIGQYLEWLDARDGD
                     WRDPARPLIRGYLGELTGRGMAKRSISSRLAALRSFYRYARREGLVAGDPWSAALTPR
                     LPRRLPQVLGVADVERLIDEADAASGDRALALRDLAMVEVAYAAGLRISELAAARLAD
                     LDLARGELRVVGKGRKERVTLLGGPARNALGNYIDHGRPLLRATTAAPAGDSGLIFLN
                     AKGAPLTTRGLRYRIDRLMLRAGLPSGASPHTLRHSFASHLLDGGADLRVVQELLGHA
                     SLGTTQVYTHVSPQRLRSAYSAAHPRSRKQTAPTP"
     gene            1505..3061
                     /gene="murJ"
                     /locus_tag="VMZ33_07560"
     CDS             1505..3061
                     /gene="murJ"
                     /locus_tag="VMZ33_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HUR17127.1"
                     /translation="MTARTLARAGMIVAAAFLLSRVLGWVRIVVITNLFDAGTELDAY
                     FAAFRLPDAIFQLVAAGALSSAMVPVLSNLFTKNEEQRAWRVVSTVLNMLLLALAAMA
                     AVVAIFAPQIVPIFTPGFDAVGTELTVRLTRIMLLSPVMLALGAVAMSVLNARGRFAA
                     AAIAPSLYNLAIIGGALLLGPYLGIESLAIGVVVGAMLHLAVQIRPLMKERFKLHFNI
                     DLSDPQARQILLLMAPRTIGLGANQITFIVATTLATGVGLGAVTAYNVAWTVLQIPLG
                     VIGFPLGVVLLPSLSRAVAAGSTKEFGTLIVRSARLVMWVMFFVTAIGIVLRRQVVTL
                     LFDYGLDPDAISLTADTLSFMFLGLAGFTLVIVFARAFYSGHDTRTPVITAVFDMVVC
                     VGIGLATIGTLGLSGVALGLAAGAWAEATALGVLLWRRTPGAGLEGIVQPMILFAIGS
                     VLAAIVALLVVRVTDSIIGPDPGKIALLGQVLAAGAGGAAAYAIYTRALRIPELAHTV
                     DLVRSMIRRK"
     gene            3058..>4517
                     /locus_tag="VMZ33_07565"
     CDS             3058..>4517
                     /locus_tag="VMZ33_07565"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HUR17128.1"
                     /translation="MTTTSDESTPTARQLGGLIQELETRGSLRTVLTPDASRVVTAVD
                     FDSRESAPGHLFVAVPGAEVDGHDYAAAAVERGAVAVIAERALPRLGVPQVLVGSSRS
                     ALAVAAAWLQGFPSYELGVVGVTGTDGKTTTSYLVRAMLDACGLGAGMITTVDVVVGG
                     QSYGETGHTTPEAPEIQRDLRRMVDAGDRFAVLESTSHGLALDRVAEVAYDVAVLTNI
                     THEHLDLHGTMEAYVGAKRSLFDRLAVGATNPDKGWPKCAIVNAEDAYADDFVRASRE
                     AGAQVVTYAVDPKVRADVSASKARDDGTGLRLVVRTPRWESELQMQLVGHFNVFNTLA
                     AVSVGEALGLDPDAMRRGLEGLDSVPGRLIAVDEGQEFTVYVDFAHTPGALAAALDSL
                     APHAAARGGGLISVFGSPGRRDVLKRPMMGRAAGERSRVVVLADDDPRDEDRMQILEQ
                     IAAGAEEVGHQRDHDLFLIPDRDRAIRKAFEMARPG"
BASE COUNT          627 a         1585 c         1521 g          784 t
ORIGIN      
        1 gagccggagg cgattgccgt cgagggggtc ggggagacct gcccggagtg caatgagggg
       61 acccttgtcg ccaagcgcgg ccgcttcggg ccgttcgcgg gttgctcgcg ctaccccgac
      121 tgcaagttca tcaagaagag cggtccgccg ccaccggatc cgctgccctt cgatgtcgtg
      181 tgcccgaaat gcaatgaggg ccagctcacg gcccggcgag cgcgacgcac aggcagcgtc
      241 ttctggggct gctctcgcta cccgaagtgt gacttcactt catcacgcga gcctctcggc
      301 gcgttccacg agaccgacga cggtccggtt gcgcgtgagg gcgaggccgg cgcgatgtgc
      361 ctgaagtgtg gtgcggccat agaggcaact ggcgagatcg tccctggttc gcgtctcgcc
      421 ggtggtgagc ccaatccaga ggccctggca ccgccgcgcc gtggccgggg aggcagcggt
      481 cgctcagcta ccgggcgtgc cgccggctcg cgccgaccga gcgcaggtgg gggcgcggcg
      541 cgaaccaacg gtcgccgagc ccgtccggct gcgtgaccgg agccgacgcg ctcgacagct
      601 tcctcgaacg gctcgctgcg cgcgactctt ctgagcacac gcgccgggca tatcgaacgg
      661 cgattggcca atacctcgag tggctcgacg ccagggacgg cgactggcgc gacccggcac
      721 gaccgttgat ccgcggctac ctcggcgagc tgaccggtcg cggcatggcc aagcgatcca
      781 tcagcagccg cctcgccgcg ctgcgttcct tttacaggta tgcacggcgc gaaggtctgg
      841 tcgcgggcga cccctggtcg gcggcactga cgccgcgcct tccccggcga ctgccgcagg
      901 tgctcggcgt cgcagatgtt gagcggctca tcgacgaggc cgatgcggcc agtggcgatc
      961 gggcgttggc actgcgcgac ctggccatgg tcgaggtggc gtatgcggcc ggtctgcgca
     1021 tcagcgaatt ggccgccgcc cgcttggccg acctggatct ggcccgcgga gagctgcgcg
     1081 tcgttggcaa aggccgcaag gagcgcgtca ccctgctggg cggacccgcg cgcaacgcac
     1141 tcggcaatta catcgatcac ggacgccccc ttttacgtgc gacgaccgcc gcccccgccg
     1201 gggactcggg gctgatcttc ctcaacgcca agggcgcgcc gctgacgacg cgtggcctcc
     1261 gctaccgcat cgatcgcctg atgcttcgcg ccggcctgcc ctccggtgcc tcgccccaca
     1321 ccctcaggca ctcttttgcg agccaccttc tcgatggcgg cgccgacctg cgcgtggtcc
     1381 aggagctgct cggacacgcg agcctgggca cgacccaggt ctatacgcac gtctcacccc
     1441 agcggctacg cagcgcatat tccgccgccc accccaggtc gcgtaagcaa accgcgccaa
     1501 ccccgtgacg gcgcgcacgc tggcgcgcgc cggcatgatt gtcgccgcgg cttttctcct
     1561 ttcgcgtgtc ctgggctggg tgcgcatcgt cgtcatcacc aacctgttcg acgccggcac
     1621 cgagctcgac gcctacttcg ccgcgtttcg gctacctgac gcgatcttcc agctggtggc
     1681 cgccggtgcc ctctcgtcgg cgatggtgcc ggtgctgtcg aacttgttca ccaagaacga
     1741 ggagcaacgg gcgtggcgcg tcgtttcgac cgtgctcaac atgttgttgc tcgcgctcgc
     1801 cgccatggcc gcagtcgtgg ccatcttcgc gccgcagatc gtgcccatct tcacccccgg
     1861 cttcgacgcc gtgggtacgg agctcaccgt ccgcctgacg cggatcatgc tcctgtcgcc
     1921 ggtgatgctg gccctcggtg ccgtagccat gagcgtgctc aacgctcgtg gccgcttcgc
     1981 tgctgccgct atcgcgccaa gtctctacaa cctcgcgatc atcggcggcg cgttgctgct
     2041 cgggccatat ctgggcatcg aatcgctggc gatcggcgtc gtcgttggcg cgatgctcca
     2101 cctcgccgtc cagatccggc cgttgatgaa ggagcgtttc aagctccatt tcaacatcga
     2161 cctgtccgat cctcaggcgc ggcagatact gctgctcatg gcgccgcgga ccatcggcct
     2221 cggcgcgaac cagatcacct tcatcgtcgc tacgactctc gccaccggcg tcggcctggg
     2281 cgcggtcacc gcgtacaacg tggcctggac ggttctgcag atcccgctcg gtgtcatcgg
     2341 cttcccgctc ggcgtcgtcc tgctgccatc cctgtcacgc gcagtcgccg ccggctccac
     2401 caaggaattc ggcaccctca tcgtgcgctc ggcccggctc gtgatgtggg tcatgttctt
     2461 cgttacggcg attggcatcg tgctccgcag gcaagtcgtg acgctgcttt tcgactatgg
     2521 cctggatccc gacgccatct ctctcaccgc cgacacgctg tcgttcatgt tcctcggcct
     2581 cgccggcttc accctggtca tcgtctttgc ccgtgcgttc tacagcggcc acgacacgcg
     2641 gacaccggtc atcacggccg tcttcgacat ggtcgtttgc gtcgggatag ggctcgcgac
     2701 catcggaaca ctcgggctgt cgggcgtggc ccttggtctc gcggccggcg cctgggctga
     2761 ggcgactgca cttggagtcc tgctctggcg tcggaccccc ggtgccggtc tggaaggcat
     2821 cgtgcagccg atgatcctgt ttgccatcgg ctccgttctg gcggcgatcg tggctctgct
     2881 cgtggtgcgc gtgaccgact cgatcatcgg acccgacccg ggcaagatcg cactgctcgg
     2941 ccaggttcta gccgccggtg ctggcggcgc cgccgcgtac gcgatctaca ctcgcgcgct
     3001 gcggatcccc gagctcgccc acaccgtgga cctcgtccgc tcgatgatca ggcgcaaatg
     3061 acgaccacgt ccgacgaatc tacgccgacc gcgcgccagc tcggcgggct gatccaggag
     3121 ctggagacgc gcggctcatt gcggacggtg cttacgcctg atgccagccg cgtcgtgacg
     3181 gccgtcgact ttgactcgcg cgagtccgcc cccggacatc ttttcgtcgc cgttcccggc
     3241 gcagaggtgg atggccatga ctacgcggcc gcggcggtgg agcgtggcgc ggttgcggtt
     3301 atcgccgagc gcgcgttgcc gcgacttggg gtgccgcagg tgctcgtcgg ttcgtcgcga
     3361 tcggccctgg ccgtggccgc ggcatggctc cagggtttcc cgtcatacga gctaggcgtc
     3421 gtcggcgtta ccggtactga cggcaagacg acgacctcat acctggtccg cgcgatgctc
     3481 gacgcgtgtg gcctcggcgc cggaatgatc acgaccgtgg acgtcgtggt cggcggccag
     3541 agctacggcg agaccgggca cacgacgccc gaggcccccg agatccagcg tgacctccgg
     3601 cgcatggtcg atgctggcga ccggttcgcg gtcctcgagt cgacttcgca cggactggcg
     3661 ctcgatcgcg tggctgaggt cgcctacgac gtcgccgtgt tgacgaacat cacgcacgag
     3721 cacctcgacc tgcacgggac gatggaggcg tacgtcggcg cgaagcggtc gctgttcgac
     3781 cggctggccg tcggggcgac caatcccgac aagggctggc ccaagtgcgc catcgtcaac
     3841 gccgaggacg cgtacgccga cgacttcgtg cgcgcgtcgc gcgaggccgg tgcgcaggtg
     3901 gtgacgtatg ccgtcgaccc gaaggtgcgc gctgatgtca gcgcgagcaa ggcacgcgac
     3961 gatggcaccg gcctgcggct ggttgtccgc acgccgcgct gggagagcga gctgcagatg
     4021 cagctcgttg gccacttcaa cgtcttcaac acgctcgcgg ccgtgtcggt gggcgaggca
     4081 ctgggcctcg atccggatgc gatgcgccgc ggccttgagg gtctggacag tgtgcccggg
     4141 cggctcatcg ccgtcgacga gggacaggag ttcaccgtgt acgtcgactt cgcgcacacc
     4201 cccggcgcgc ttgcggccgc gctggactcg ctcgccccgc acgccgccgc tcgcggggga
     4261 gggctgatct ccgtgttcgg ctcgcccggc cgccgcgacg tcctcaagcg gccgatgatg
     4321 ggccgggccg cgggggagcg atcacgcgtc gttgtgctcg ccgacgacga cccgcgcgac
     4381 gaggaccgga tgcagatcct ggagcagata gcggccggcg ccgaggaggt cggccatcag
     4441 cgcgaccacg acctgttctt gatccccgat cgcgacaggg cgatccgcaa agcgttcgag
     4501 atggcacgac cgggcga
//
LOCUS       DATLCO010000329         5224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8608772, whole genome shotgun sequence.
ACCESSION   DATLCO010000329 DATLCO010000000
VERSION     DATLCO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8608772"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VMZ33_07570"
     CDS             <1..237
                     /locus_tag="VMZ33_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17129.1"
                     /translation="QHNATIVLTTHDLAEAERLCSRIGLLNEGRLVAEGTVAQLTAGV
                     AAAHGDGRPTTLHDVFMAYTGRSLDDDVEKDDDD"
     gene            complement(234..467)
                     /locus_tag="VMZ33_07575"
     CDS             complement(234..467)
                     /locus_tag="VMZ33_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17130.1"
                     /translation="MTCRLGSQTAADPDHVPADNVADPTLWTTGSNEPLSTSHVPTSL
                     MATPAFYLRMEGMDQQFEFLFLESAVASQSPAC"
     gene            522..1328
                     /locus_tag="VMZ33_07580"
     CDS             522..1328
                     /locus_tag="VMZ33_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR17131.1"
                     /translation="MTAATLTRELRASYAFVERNFNLSKRYWGWEVAFMVYSVAGALS
                     VTLIGLDQASNRLILSLMIGATFWNYLSVVFSIIAEQISWERWEGTLEYTMMAPVRRV
                     SQLMGSTFFAIAWGLIHTTVVLTALVLFFGIDLSNADFITAGALMAVGSLSIIGVGMM
                     AAALPLLWVERGDQMVFVLNSLLLLISGVYYSVTILPGWMQAISALSPATYILDGIRQ
                     ALIEGVPVTQMGGYLLILAVMACIFIPLGIWVFSRAERYAKRTGKLKRVG"
     gene            1359..2384
                     /locus_tag="VMZ33_07585"
     CDS             1359..2384
                     /locus_tag="VMZ33_07585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR17132.1"
                     /translation="MKTMDRSAGSVRKAPDGLRLRPYAGESDIPTIANIINRELEHDG
                     VPFRESEADIVAWYGHPNDKFDSARDVTIAEVDGVPVGFAQRSWIDTTLEEFREYRND
                     GGVLPEWQRRGIGTALLEYNIERSQALAATHETNRPRILGSWTADRMPGSQAILRAAG
                     FEQVRWFFEMTRDLSLPIPEVPMPEGLEVREVTTDQIRQIWEADIEAFLDHWGGFDGS
                     EDSYQRFLQKPAFDPSLWVIAWDGDEVAGGVVNAIETAENEMLGVKRGWLHSVFTRRA
                     WRKRGLAHALITRSLVKIKERGMDQGILGVDASNPTGALGLYERNGFVVAERSTAWRR
                     PLFEDQR"
     gene            2381..3472
                     /gene="rsgA"
                     /locus_tag="VMZ33_07590"
     CDS             2381..3472
                     /gene="rsgA"
                     /locus_tag="VMZ33_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HUR17133.1"
                     /translation="MSEPMDLGQYGWNASVADRFKRHVAEDLRPARVLAEHRGSYALV
                     TTTGEIDGSVSGRFRYDASSAEEFPAVGDWVGIEMGAAGAAAIIRAVLPRQTQFARPA
                     RGDQPGAQVVAANVDVVLVVSGLDDDFNIRRLERFMTLAWSSGAEPVIVLNKADLCED
                     IAARLADVAAIAPGVPIRVLSARDGTGIESLTPLLEPGKTIALLGSSGVGKSTIVNAL
                     LGWERQVTKDVREDDARGRHTTTSRELMVMPSGALLIDNPGIRSVGVWDADEGLDDAF
                     ADVQAFAAECRFNDCTHEGEPGCAVRAAIESGQLPAERLASQQHLTREWAALARRVDP
                     NARANERARWKAIHRSVRNHMKQKYEAEA"
     gene            complement(3539..4177)
                     /locus_tag="VMZ33_07595"
     CDS             complement(3539..4177)
                     /locus_tag="VMZ33_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR17134.1"
                     /translation="MKLLQRVIGPIATNLYVLGDEASGEAIAIDTATPCVEWLTTTLA
                     ERGWTLKLIVSTHRHWDHIGDNASVSDKTGAQIAAHALDRHGLEHPQGFNLPFPIVPS
                     VPAVDLAEGYQIRFGEIDLEVLHTPGHTEGSVCLLARSQRLLFTGDTLFAGSWGRVDL
                     PGGSEDQMIESLTRLAGMDTDLGVLPGHGNKSTIDAEQAWLRAVAEVRRLPF"
     gene            complement(4196..4648)
                     /locus_tag="VMZ33_07600"
     CDS             complement(4196..4648)
                     /locus_tag="VMZ33_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17135.1"
                     /translation="MMSRFWRTCFGTVYRLLAWLDPLIRSTWRRMGIGNVVELRVAAR
                     NNPGTQRSRMIGTLLAGGHTYIGHPDGDSAWTRDLRAAQRAVIRYHSGGEWPVRATLL
                     DPTSDPEERELAIRATGQHPFPGNLIYRLGRRHVRATGVYFRLDDDLT"
     gene            4680..>5224
                     /locus_tag="VMZ33_07605"
     CDS             4680..>5224
                     /locus_tag="VMZ33_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR17136.1"
                     /translation="MSNGATFTWYGHSCVELRTPAGKTILLDPWFGNPTSPRSAEVVD
                     ACDLMLVSHGHFDHLGSGPRDVGNADALTIARRTKPAWPCIHELSLWLETQLDSESGV
                     EIIGMNKGGTVAARGLEVTMVHAEHTAGDWSTSGDGPLYLGEPVGFVIELEDGKRVYY
                     TGDTDVFGDMDLVRRLHKPDVA"
BASE COUNT          917 a         1679 c         1731 g          897 t
ORIGIN      
        1 cagcacaacg cgacgatcgt cctcacgacg cacgacctcg cagaggccga gcgtctgtgc
       61 tcccgcatcg gtctgctcaa cgagggccgg ctggtagccg agggcacagt cgcacagctc
      121 acagcgggcg tcgccgccgc acacggcgac ggccgaccaa ccaccttgca cgacgtattc
      181 atggcctaca cgggccgttc gctcgacgac gacgtcgaga aagacgacga cgactagcaa
      241 gcaggcgact gcgaagccac agctgattca agaaagagga actcgaattg ttgatccatc
      301 ccctccatgc ggagatagaa agccggcgtc gccatgagcg acgtcgggac gtggctcgtc
      361 gacagcggct cgttgctgcc ggtcgtccac agagtcggat ccgccacgtt gtcggccggc
      421 acatgatccg gatcggcagc cgtttggctg ccgagccggc acgtcaagtg gctcgctccc
      481 aataggggcg agccacacca cctctcggag aacccgctgc aatgaccgcc gccacactga
      541 ctcgcgagct gagggcttcc tacgccttcg tcgagcgcaa cttcaacctg tccaagcgct
      601 actggggctg ggaagtggcc ttcatggtct actcggtcgc tggcgcacta tcggtgacgc
      661 tcatcggcct cgaccaggcc agcaatcgcc tgatcctctc gctgatgatc ggagccacgt
      721 tctggaacta cctgtcggtg gtcttctcca tcatcgccga gcagatcagc tgggagcgct
      781 gggaagggac cctcgagtac accatgatgg cgcccgtccg gcgcgtgtcg cagctcatgg
      841 gctcgacatt tttcgccatc gcctgggggc ttattcacac caccgtcgtc ctgacggcgc
      901 tggtgctgtt cttcggtatc gacctgtcaa acgccgactt catcaccgcc ggcgcgctga
      961 tggccgttgg ctcgctctcg atcatcggcg tgggcatgat ggctgcggcg ttgccactgc
     1021 tctgggtaga gcgtggcgac cagatggtct tcgtgctcaa ctcgctgctg ctgctcatca
     1081 gcggcgtcta ctacagcgtc acgatcctgc ccggttggat gcaggcgatc tcggcgctgt
     1141 cacctgcgac atacatcctc gatggcatcc gccaggcgct aattgaaggc gtgcccgtta
     1201 cacagatggg cggctacctg ctgatcctgg ctgtgatggc gtgcatcttc attccgctcg
     1261 gcatctgggt attcagccgc gcggagcgat acgccaagcg gaccggcaag ctcaagaggg
     1321 tcggctagat gctggctgat caggaaggac tgaccgacat gaagaccatg gaccgttccg
     1381 ctggctcggt gcgcaaggcc cctgacggcc tgcgtctccg tccgtatgcc ggcgaatcgg
     1441 atatcccaac catcgccaat atcatcaacc gcgagctcga acacgatggc gtgcctttcc
     1501 gcgagtcgga ggcggacatc gttgcctggt atggccaccc caacgacaag ttcgactccg
     1561 cgcgggacgt cacgatcgcc gaggtcgacg gcgttccagt cggctttgcc cagcggagct
     1621 ggatcgacac aacgctcgag gagttccgcg agtaccgcaa cgacggcggc gtgctgcccg
     1681 agtggcaacg ccgcggtatc ggtacagcgc tgctcgagta caacatcgag cggagccaag
     1741 cactggcagc cacccatgag acgaatcggc cgcgcatcct gggcagctgg accgccgacc
     1801 gcatgcccgg ctcgcaggcc atcctgcgcg ctgccggctt cgagcaagtg cgctggttct
     1861 ttgagatgac gcgcgaccta tcgctaccga ttccggaggt gcccatgccc gaaggtttgg
     1921 aggtgcgcga ggtcacgacg gatcagatcc gccagatctg ggaggccgat attgaggctt
     1981 tcctcgatca ttggggcggc ttcgacgggt cggaagacag ctaccagcgc ttccttcaaa
     2041 agccggcttt cgacccatcg ctgtgggtca tcgcatggga cggcgacgag gtcgcaggcg
     2101 gtgtcgtcaa cgccatcgag accgccgaga acgagatgct cggcgtcaag cgcggctggc
     2161 tccacagcgt cttcacgcga cgtgcctggc gcaagcgcgg tctcgctcac gccctcatta
     2221 ctcgctccct ggtgaagatc aaggagcgcg gcatggacca gggaatcctc ggcgtcgatg
     2281 cctccaatcc caccggcgcg ctgggactct acgagcgcaa cgggttcgtg gtcgccgagc
     2341 gttcgacggc ctggcggcgg ccactgttcg aggaccagcg gtgagcgaac ccatggatct
     2401 gggccaatac ggctggaacg cctcggtcgc ggaccgcttc aagcgacacg tggccgagga
     2461 cctccggcct gcccgcgtcc tggccgagca tcgcggctcg tacgcgctgg tcacgacgac
     2521 tggcgagatt gacggctcgg tctcagggcg tttccgatac gacgcatcgt ccgcggagga
     2581 attcccggcc gtgggtgact gggtcggcat cgagatgggc gcggcggggg cggccgccat
     2641 cattcgagcc gttctgccgc gtcagacgca gttcgcgcgg cccgcgcgcg gcgatcagcc
     2701 tggggctcag gtcgtcgccg cgaacgtcga tgtggtgctc gtcgtaagtg gcctcgacga
     2761 tgacttcaac atccggcggc tcgagcgctt catgactctg gcgtggtcga gtggcgccga
     2821 acctgtgatc gtcctcaaca aggccgatct gtgtgaggac atcgccgccc ggctggccga
     2881 tgtcgcagcc atcgctccgg gcgtgccgat ccgcgtcctc agcgcgcgcg acggcacggg
     2941 catcgagtcg ctcactcccc tccttgagcc gggcaagacg atcgcgctcc tcgggtcgtc
     3001 gggagtcggc aagtcgacga tcgtgaacgc gctcctcggc tgggaacggc aggtcaccaa
     3061 ggacgtgcgt gaggacgatg cccgcggtcg ccacacgacg acgagccgcg agctgatggt
     3121 gatgccgagc ggcgcgctgc tgatcgacaa cccgggcatc cgctcggtcg gcgtttggga
     3181 cgccgacgaa gggctcgacg atgctttcgc tgacgtccag gctttcgcgg cggagtgccg
     3241 cttcaacgac tgtacgcacg agggcgagcc cggttgcgcc gtacgtgcgg cgatcgaatc
     3301 gggccaactg ccggcggaac gcctggccag ccagcagcac ttaacccgcg aatgggcggc
     3361 actcgcacgg cgggtcgatc cgaacgctcg ggccaacgaa cgcgcccgat ggaaggcaat
     3421 ccacaggtcg gtgcggaacc acatgaaaca gaagtacgag gctgaagcct gagcgtaggc
     3481 tctgcgcgcc gacagcggcc gtgcaagccg ttgcccaggc cgtcctggcc gcgtgcccct
     3541 agaaagggag gcggcgtacc tcggcaacgg ccctgagcca ggcctgttcg gcatcgatgg
     3601 tggacttgtt gccgtgtccc gggaggacgc ccagatctgt gtccatgccg gccagccggg
     3661 tgagcgactc gatcatctga tcctccgacc cgccgggcag gtcgaccctc ccccaactgc
     3721 cggcgaacag cgtgtcgccc gtgaacaaca gccgttgaga gcgagccagc aaacagaccg
     3781 aaccctcggt gtggccgggc gtgtgcagga cctcaaggtc gatctcgcca aatcggatct
     3841 ggtagccctc cgccaggtcg actgctggca ctgagggcac gatcgggaat ggcaagttga
     3901 agccctgcgg gtgctccagg ccgtgacgat cgagcgcgtg cgcggcaatc tgggcgccag
     3961 tcttgtcgga caccgaggcg ttgtcaccga tgtgatccca gtgtcgatgg gtcgaaacga
     4021 tcagcttcaa cgtccagccg cgctcggcga gcgttgtcgt gagccactcg acgcacggcg
     4081 ttgccgtgtc tatggcaatg gcttcgccgc ttgcctcgtc gccgagcacg tagagattgg
     4141 tggcgatggg gccgatgacc cgctggagga gcttcaagcg ctgaaggcta gcgcctcacg
     4201 tgaggtcgtc gtcgaggcgg aaataaacgc cggttgcacg gacatgtcgg cggccgagtc
     4261 ggtagatcag gttccccgga aacgggtgct ggcccgttgc gcggatcgcg agctcacgtt
     4321 cttcggggtc gctggtggga tccaggagcg tcgctcggac aggccattcc ccgcccgagt
     4381 ggtagcggat gactgcacgc tgggcggcgc gaaggtctcg cgtccaggcg gagtcgccat
     4441 cgggatggcc gatgtacgtg tgcccgccgg ccaggagagt gccgatcatc cggctgcgct
     4501 gggtgcccgg attgttacgc gccgcaacgc ggagctcgac gacgttgccg atacccatcc
     4561 ggcgccacgt tgatcggatc agcgggtcga gccaggcaag caggcgatat accgtgccga
     4621 agcaggtacg ccaaaatcga gacatcacat aggtatcttc ggcgggaagg aaggcttgga
     4681 tgagcaacgg ggccacgttc acctggtacg ggcattcatg cgtcgagctg cgcaccccgg
     4741 ctggcaagac gatcctgctg gatccctggt tcggcaaccc cacttcgccg cggtcggcgg
     4801 aggttgtgga tgcctgcgat ctgatgctcg tctcgcacgg gcacttcgac catctcggct
     4861 cgggcccgcg ggacgtaggc aacgccgatg ccctaactat tgcccggcgt accaagccag
     4921 cctggccgtg catccacgag ctgtcgctgt ggcttgagac gcaactcgac tcggagtccg
     4981 gtgtcgagat catcggcatg aacaagggcg gaaccgtcgc cgctcgtggc ttggaagtga
     5041 cgatggtcca cgccgagcac actgcaggtg actggtcaac gagcggcgac ggacccctgt
     5101 acctgggcga gcccgtcggg ttcgtgatcg agctggagga cgggaagcgc gtctactaca
     5161 cgggcgacac ggacgtgttc ggggacatgg acctcgtccg acgcctccat aagcccgacg
     5221 ttgc
//
LOCUS       DATLCO010000330         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3419375, whole genome shotgun sequence.
ACCESSION   DATLCO010000330 DATLCO010000000
VERSION     DATLCO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3419375"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VMZ33_07610"
     CDS             complement(<1..887)
                     /locus_tag="VMZ33_07610"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUR17137.1"
                     /translation="MRGSRGRAWPVRLAALLLVVTQAVAFAAPAAAETSPDFDPALAA
                     KLQAILDRVRIERPIAGLAAVVTLADGSRWEGGSGFGDAFDGSQLFSAHTPSVAGSIT
                     KTFVAALILQLVEEGKLHLDDRLDDWVPEKGFSPRVRIKHLLRHTSGIPDYFRHPDYN
                     RLVFNRPTHVWSKAEVMSLIKTTLRFTPGHSWSYSNSGFFLLGLIAERATHSTLGALL
                     RQRFFVPLGLDETWLQGEHTLPTNSAKGYLWVNGRFKEYADGTSFRPHTSAATVAWGA
                     GNILTTASDLSDWVRALYGG"
     gene            complement(941..>2083)
                     /locus_tag="VMZ33_07615"
     CDS             complement(941..>2083)
                     /locus_tag="VMZ33_07615"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HUR17138.1"
                     /translation="GFTVPAVNLRTQVFDMVGAMCRAAQSLDCGTIVFELARSEQEYT
                     FQRPAEFITSVLAGCVAANWQGPVFVQGDHYQFNARKYAVDPEATAEGVRKLARDAVA
                     VGYGNIDIDSSTLVDLGRPTVDDQQRVNYERAAEISALIRANEEPGSTISIGGEIGEV
                     GKQNSTEEELRAYLEGYRAQLRHLNGDVPGLSKVSVQTGTSHGGVPLPGGGVAEVKLD
                     FGTLERLGHVAREYGLAGAVQHGASTLPDELFNRFPEVETAEIHLATGFQNMLYEHPA
                     FPAELLREVEAWTIANTADERVAGESDTQFFYKTRKKALGPMKRRLWELETKDEIIAT
                     QEEKFRFLFEQLRVTGTRGMVERYISAPRITRPIPATLRQAVTAAG"
BASE COUNT          357 a          664 c          688 g          374 t
ORIGIN      
        1 ccgccatata gcgcccggac ccagtcggac aggtcggatg cagtcgtcag aatattgccg
       61 gcaccccagg cgaccgtggc cgccgatgtg tggggccgga agctggttcc gtccgcgtat
      121 tccttgaacc tgccgttcac ccagagatag cccttggccg agttggtggg caaagtgtgc
      181 tcgccctgga gccacgtctc gtccagtccg agtggaacga agaaacgctg gcgcagcagt
      241 gccccgagcg tcgaatgagt cgcacgctcg gcaatcaggc ccagcaggaa gaagcccgag
      301 ttcgagtacg accagctatg gcccggcgtg aagcgcagag tcgtcttgat caggctcatg
      361 acttcggctt tcgaccatac gtgcgtcgga cggttgaaga cgagacggtt gtagtcgggg
      421 tgcctgaagt agtcgggtat cccgctcgtg tggcgcagga ggtgcttgat gcgcacgcgc
      481 ggcgaaaagc ccttttcggg gacccagtcg tccaggcggt catccagatg caacttgcct
      541 tcttcgacca gttggaggat gagcgccgcg acgaaggttt tcgtgatgct gccggcgacc
      601 gagggcgtat gggcgctgaa cagctggctg ccatcgaacg catcgccgaa tccgctacca
      661 ccctcccagc gcgagccgtc ggccagcgtg acgacggcgg ccagccccgc gatcggtcgc
      721 tctatgcgaa ctcgatcgag gatggcttgc agctttgccg caagggccgg atcgaaatcg
      781 ggcgaggtct cggccgcagc gggcgccgcg aaggcaactg cttgtgtgac gacgagcagc
      841 aaagctgcca gtcgcaccgg ccatgcgcgc cccctcgatc ccctcatcgt tacctcagat
      901 tatccgacca gctgggctgc gacggcccat tgggaagtcg ctagccggcg gctgtaaccg
      961 cttggcggag tgtggcgggg atagggcggg taatgcgagg agcggagatg taccgctcga
     1021 ccatgccgcg agtaccggtg acacgcagct gttcgaacag gaagcggaac ttctcctcct
     1081 gcgtcgcgat gatctcgtcc ttggtctcga gctcccacag acggcgcttc atcggcccga
     1141 gcgccttctt ccgggtcttg tagaagaact gcgtatcgct ctcgccagcg acccgctcat
     1201 cggccgtgtt ggcgatcgtc catgcctcga cctcgcgcag cagctcggcc ggaaaagcgg
     1261 ggtgttcgta gagcatgttc tggaagcccg tggcgaggtg gatttcggcc gtctcgacct
     1321 cagggaagcg gttgaagagc tcatcaggca gcgttgacgc gccgtgctgg acagcgccgg
     1381 caaggccgta ttcgcgggcg acgtggccga gccgctcgag cgtgccgaag tccagcttca
     1441 cctctgcgac accgccgccg gggagtggca cgccgccatg cgatgtgccg gtctggacac
     1501 tcacctttga gaggccgggg acgtcaccat tgagatggcg cagctgggct cgatagccct
     1561 ctagataggc gcgcagctcc tcctcggtcg agttctgctt gccgacctcg ccgatctcgc
     1621 caccgatgct gatcgtgctg cccggctcct cgttggcccg gatgagagcc gaaatctctg
     1681 cggcgcgctc gtaattgaca cgctgctggt cgtcgactgt cggccggccc aggtcgacca
     1741 gggtcgacga gtcgatgtcg atgttcccgt agccgacggc cactgcgtcg cgcgccagct
     1801 tccgaactcc ctcggcggtc gcttcggggt cgacggcgta cttcctggca ttgaattggt
     1861 agtggtcccc ctgcacgaaa acgggaccct gccagttcgc cgcgacgcaa ccagccagga
     1921 cgctcgtgat gaactcggcg ggacgctgga acgtgtactc ctgctcgctg cgcgcgagct
     1981 cgaacacgat cgtgccgcag tccagcgact gcgccgcccg acacatggcg ccaaccatgt
     2041 cgaacacctg cgtccggagg ttgacggcgg gcacggtgaa gcc
//
LOCUS       DATLCO010000331         4514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6359039, whole genome shotgun sequence.
ACCESSION   DATLCO010000331 DATLCO010000000
VERSION     DATLCO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6359039"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VMZ33_07620"
     CDS             complement(<1..419)
                     /locus_tag="VMZ33_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUR17139.1"
                     /translation="MAEGERAQGTLYVVATPIGNLGDITLRALEVLRAAPLVAAEDTR
                     MTRRLWARHGIATRLISYHAQSGIRRRDELLAHLAGGADLAIVTDAGTPLVSDPGEEL
                     VAAWAARGGRVVPIPGASAVLAALVASGLPAARWCFEG"
     gene            complement(412..1293)
                     /locus_tag="VMZ33_07625"
     CDS             complement(412..1293)
                     /locus_tag="VMZ33_07625"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HUR17140.1"
                     /translation="MNNVSPLPYTLPEELSIDTSIARRIIVEFLSRQLEQAGFDRLVL
                     GLSGGLDSALVCYLATEAIGADNVLCLLMPYRTSSPESEADARAVVAALGCPSELVDI
                     TPIVDGYFATPLPAGVEVTRGRRGNFAARARMAVLYDRSATWRGLVVGTGNKTETLLG
                     YTTIYGDSAAALLPIADLYKSQVRQLSGDMGVPEQILRKPPTADLWPDQTDEGELGIK
                     YEDLDRLLYWMIDRRRTDEQLEAMAFPQDRVAHVKGMVARAEYKRQMPPVAKLTTRTP
                     GVDYLYPRRRPGSRAGG"
     gene            complement(1299..2177)
                     /locus_tag="VMZ33_07630"
     CDS             complement(1299..2177)
                     /locus_tag="VMZ33_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HUR17141.1"
                     /translation="MLRVGLGQLAPGLGLLEKNLAAHARVIDEARESGVNLLVFPELG
                     LTGYLLQDLNAEVAMRADDARLQELAARAPDMSVVVGFVEESVDHRLYIAAALLEGGA
                     VSHVYRKSYLPNYGLFDERRFFAAGSQVRAVDSALGVRLGICICEDFWHLPIPYLLSL
                     DGAQVLINIASSPGRDIATTVDDLGSAQSWRTLMRAYAQLTTCAIVFVNRVGVDETVT
                     FWGGSQVIAPSGAVVYEAPLLEEGLFIAELDMTEVRRERIALPVLRDERPDFVLRQLE
                     RIIRDDAGVATDTQAE"
     gene            2386..4326
                     /locus_tag="VMZ33_07635"
     CDS             2386..4326
                     /locus_tag="VMZ33_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR17142.1"
                     /translation="MVNKPRWLTFATTGLVAMMVISACSAGPAATTAPSTGPSTAPSA
                     APSTAPSTAPSEAPSVEPSAEPSVEPSVEPSVEPSVEPSVEPTAAPIGTAYPDPAATP
                     GDAAEISNVYPNYGEPIDCESETWNGLPYAGSVRSIEAPDPATVVFNLCAPDVAFLSK
                     MAFSVFAINDKDFLVEHTADQTIQNVLNGTGAYALNEWRRGDSILFDRFDGYWGDPAA
                     TATGVLRWQPEAAARLQELQSGTIDGMTLVGPTDFETVSNNPDLQLKPSGGLNTLYLG
                     MNHDVAPWDNQAVRQAIAIGIDRQRIVDNFLPPGSEVATHFTPCTIPFACVGDAWPDT
                     NVDAARAAITEEFGEAGLTTTLSYRNVARGYIPQPVETATDVQAQLEAINIHATLDEQ
                     ESTTFLDNANSGSLTGLFLLGWGADYPDVTNFLDYHFGSGCTSAFGACYPEIFDHLTV
                     GAQTNDEAAREAAYVDANNAISEQVPMVPISHAGFANAYLAGVEHAQVSPLSNELLYR
                     MVPADGGDQVVFMQAGEPGGLYCADETDGESLRACEQSMEGLYGFAEDGTEPVPALAT
                     ECTPNEDLSVWTCALRQGVTFHNGATFEAEDVIASFAAQWDVLSPLHVGRTSDFEYWS
                     ALWGFLNPAAPCGIEGQPACEE"
BASE COUNT          798 a         1541 c         1442 g          733 t
ORIGIN      
        1 ccttcaaaac accagcgcgc agccggcaac ccgctcgcca ccagcgcagc gagcacggcc
       61 gatgcaccag ggatcggcac cacgcgcccg ccacgtgccg cccacgccgc gacgagctcc
      121 tcgcccgggt cgctcacgag cggcgtgccg gcatcagtca caatggccag gtccgcgcca
      181 ccggcgaggt gcgccagcag ctcgtcgcgt cgtctgatgc cactctgcgc gtggtagctg
      241 atcagccgcg tggcgattcc gtggcgtgcc cacagccgtc gcgtcatgcg cgtgtcctcg
      301 gccgcgacca gcggcgctgc tctgagcacc tccagggccc tgagggttat gtcacccagg
      361 ttcccgatgg gagtggccac gacatacagc gttccctggg cgcgctcccc ctcagccacc
      421 ggcgcgcgag ccggggcgcc gccgcgggta gaggtagtcc acgcccggtg tgcgcgtggt
      481 gagcttggcg accggtggca tctggcgctt gtactcggcg cgcgcgacca tccctttcac
      541 gtgcgcgacc ctgtcctggg ggaaagccat ggcctcaagc tgctcgtcgg tccgccgacg
      601 gtcgatcatc cagtagagca gccgatcgag gtcctcgtac ttgatgccca gctcgccctc
      661 gtcggtctga tcgggccaca aatccgccgt cggcggcttg cgcaggatct gttcgggaac
      721 gcccatgtcg cccgaaagct ggcggacctg gctcttgtac aggtcggcaa tcggcagtag
      781 ggccgcggcg ctgtcgccgt agatcgtcgt gtaaccgaga agcgtctctg tcttgttgcc
      841 cgttccgacg acgaggccgc gccatgtcgc cgaccggtcg tacaggactg ccatccgagc
      901 gcgcgccgca aagttgccgc gccgcccgcg cgttacctcc acacctgctg gaaggggcgt
      961 tgcgaagtag ccgtcgacga tcggggtgat gtcgacaagc tcgctcgggc aacccaacgc
     1021 ggcgacaacg gcgcgagcat ccgcctcaga ttcgggtgaa gacgtccggt agggcatcag
     1081 caggcacagg acgttgtcgg cgccgatggc ctctgtcgcg agataacaca cgagtgccga
     1141 atcgagcccg cccgataacc cgagcaccag gcgatcgaag cccgcctgct cgagctggcg
     1201 tgagaggaac tcaacgatga tgcgtcgcgc gatggaggtg tcgatcgaca gctcctcggg
     1261 cagcgtgtag ggcagcggcg agacgttatt caccagcgtt attcggcctg cgtgtcggtt
     1321 gccacaccgg cgtcgtcgcg gatgatccgc tcgagctgac gcagcacgaa gtcaggccgc
     1381 tcgtcacgca acacgggcaa cgcgattcgc tcgcgtcgca cctcggtcat gtcgagctcg
     1441 gcgatgaaca gcccttcttc gaggagaggc gcctcgtaga ccaccgcgcc cgacggcgcg
     1501 atcacttgcg agccacccca gaaagtgacg gtctcgtcga cgcccacccg gttcacgaaa
     1561 acgatcgcgc acgtggtcag ctgcgcgtag gcgcgcatga gggttcgcca cgactgggcc
     1621 gaaccaagat cgtcgacggt ggtcgcaatg tcacgccccg gcgacgacgc gatgttgatg
     1681 agcacctgag caccgtcgag ggagagcagg tacggaatcg gtaaatgcca gaagtcctcg
     1741 cagatgcaga tccccagccg gacaccgagc gccgagtcga cggcgcgtac ctggctgccc
     1801 gccgcaaaga accgccgctc gtcgaagagc ccgtaattgg ggaggtacga cttgcggtag
     1861 acgtggctga ccgcgccgcc ctcgaggagc gccgctgcga tgtagagccg atggtcgacc
     1921 gactcctcga cgaagccgac gaccacgctc atgtcagggg cgcgtgccgc aagctcctgc
     1981 aagcgggcgt cgtcggccct catggcgacc tcggcattca ggtcctggag caggtagccg
     2041 gtgaggccga gctccgggaa cacgagcagg ttcacgcccg actcgcgcgc ttcgtcgatg
     2101 acacgcgcgt gggcagcgag gttcttctcg aggaggccca gacccggtgc gagctggccg
     2161 agccctacgc gcagcatcgt cacgaggctg atggtattcg gcggaagacg ttaagtggcc
     2221 taaatgttgt ggcacggcgc atcctcgcgg tgctagcctt cccggacctg tcgccaggcg
     2281 aaggtcgccg ccccgtgcgc gcgattttcc aatgtgggca cgaggccgcg gtctcgggga
     2341 gcgcccagac agacaggtga aacacacaga ggaggaacga cccgcatggt caacaaaccc
     2401 cggtggctga catttgccac cacagggctc gttgcgatga tggtgatcag cgcatgtagc
     2461 gctggcccag cagcaacgac tgccccttcg acggggccct cgacggcacc gtccgcggcg
     2521 ccctcaacgg cgccgtccac tgcccccagc gaggcgccaa gcgttgagcc gagtgccgag
     2581 cccagcgttg agccgagtgt cgagcccagc gttgagccga gtgtcgagcc cagcgttgag
     2641 ccgaccgccg cgcccatagg cacggcttat ccggacccgg cggcaacgcc aggcgacgca
     2701 gctgagatca gcaacgtcta tccgaattac ggtgagccga tcgattgcga gtccgagacc
     2761 tggaacggcc tgccctatgc cggcagcgtc cgctcgatcg aggctccgga cccggcaacc
     2821 gtcgtcttca acctgtgcgc gcctgacgtc gcgttcctgt caaagatggc gttctcggtg
     2881 ttcgcgatca acgacaagga cttcctcgtt gagcacaccg ctgaccagac gatccagaac
     2941 gtcctgaacg gcaccggcgc atacgctctc aacgagtggc gccgcggcga cagcatcctg
     3001 ttcgaccgct tcgacggcta ctggggcgac ccggcagcca cggcgactgg cgttctgcgc
     3061 tggcagcctg aggctgcggc acgactgcag gagctccagt cgggcacgat tgacggcatg
     3121 acgctggttg gtccgaccga cttcgagaca gtcagcaaca accctgattt gcagctgaag
     3181 ccgtccggcg gcctcaacac gctgtacctg ggcatgaacc acgacgtcgc gccgtgggac
     3241 aaccaggcag tgcggcaggc catcgcgatc ggcatcgacc gccagcgcat cgttgacaac
     3301 ttcctgccgc cgggctccga agttgccacg cacttcactc cgtgcaccat cccgttcgcc
     3361 tgtgtgggcg acgcgtggcc ggacacaaac gtcgacgcag ctcgcgccgc tattaccgaa
     3421 gaattcggtg aggccggcct gaccacgacc ctcagctatc gcaacgtggc ccgcggttac
     3481 atcccgcagc ccgttgagac agcgaccgac gtccaggctc agctcgaggc gatcaacatc
     3541 cacgcaacac ttgacgagca agagtcgacc accttcctcg acaacgcgaa tagcggttca
     3601 ctgacggggc tcttcctgct cggttggggc gctgactatc cggacgtaac caacttcctc
     3661 gactaccact tcggctcagg ctgcacgagc gcgttcggcg cctgctatcc ggagatcttc
     3721 gatcacctca ccgttggcgc acagaccaac gacgaggctg cacgcgaagc ggcatatgtc
     3781 gacgcgaaca acgccatcag cgagcaggtt ccgatggttc cgatctcgca cgcaggcttc
     3841 gcgaacgcct acctggctgg tgtcgagcat gcccaggttt cgccgctcag caatgagctg
     3901 ctgtaccgca tggtcccggc tgacggaggc gaccaggtcg tcttcatgca ggccggcgag
     3961 ccgggcggcc tctactgcgc cgacgagacg gacggcgaga gcctccgtgc ctgcgagcag
     4021 tcgatggaag gcctctacgg cttcgccgaa gatggcaccg agccggttcc ggctcttgcc
     4081 acggagtgca ccccgaacga ggacctgtcc gtctggacat gcgcccttcg ccagggcgtg
     4141 acattccaca acggcgcaac cttcgaggct gaggacgtca ttgcgtcctt cgccgctcag
     4201 tgggatgtcc tgagcccgct ccacgtgggt cgcacgagcg acttcgagta ctggagcgct
     4261 ctctggggct tcctcaaccc tgccgctccg tgcggcatcg agggtcagcc cgcctgcgaa
     4321 gagtaggcag tacctctcgc acctgaacgg aggggcgccg gccgcaaggc tggcgcccct
     4381 tcgcgttaca ttgctcgagt acaggtgagc attcgctaga tgatccgctt catcattcgc
     4441 cggctcgtcg tcagcattcc ggtgctgatc ggcgtcgtcg cggtcatctt tgtcctcacc
     4501 cgcgtcatcc ccgg
//
LOCUS       DATLCO010000332         5231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7852020, whole genome shotgun sequence.
ACCESSION   DATLCO010000332 DATLCO010000000
VERSION     DATLCO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7852020"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VMZ33_07640"
     CDS             <1..891
                     /locus_tag="VMZ33_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HUR17143.1"
                     /translation="GSGALEDGIIKDSTLVETAGYIEIGTYRYYDWNRRGFGPLDIYG
                     GFAHSSDTYFYQLAGTLGIDRLGQWASEWGFGQRTGIDLPAEARGIVPTNEWKQNLFS
                     EPIYPGEVYQAGIGQGYDAATPLQVLNAYNALANGGNLLKPQIVRRVLAPDGTVVSEF
                     QPELIRELDVSKDTLRIMRLAAREVVTSRHTFNLVDLPFVVAGKTGTAEFGLRDDKGR
                     LPYHTWFAAFVPNFTETQEGNPEATDSELAILAFAYDSNTKGNAAVEIVKYFLQDHFD
                     VGVDLTRPDLLAKGNFYGGH"
     gene            997..2151
                     /gene="rodA"
                     /locus_tag="VMZ33_07645"
     CDS             997..2151
                     /gene="rodA"
                     /locus_tag="VMZ33_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HUR17144.1"
                     /translation="MSVMRIERRRADSLLARPESSAWQRFDFQLVFYAAALATIGLLV
                     AWTNSAGSPLSPGSVFTRGLMWFSIGIVVFTAAVAFDYRWLRAFSPLLYLINIGLLVM
                     TLAIGTGVGGVQRWVSIFGLQFQFSEISKILMIAVLAAWMHARRDKLGSLSTILGAGL
                     LTLPPFVLVMIEPDLGTSLVFLAILLGTMFMTGASLRWMGIGLATGIAALPVAWSQLA
                     DYQRRRVLSFLDPTADTTGAGWQALQSQIAVGSGGIFGKGLTNGSQSSSDFLPVQSTD
                     FAFAVLLEELGFVGGMVVFLLFVGLVWRVLVIGWRSESAFGVAFCAGVASMIVFQLLV
                     NAGMVAGIMPVTGIPLPFITHGGASLTSIAIALGLVQSINIREPRGSPVD"
     gene            complement(2148..2612)
                     /locus_tag="VMZ33_07650"
     CDS             complement(2148..2612)
                     /locus_tag="VMZ33_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HUR17145.1"
                     /translation="MTTRDPRHTYYKTLMLAETADAEIIGVVYRKLAQRFHADVDPSP
                     EAARRMAEINVAHEVLSDAQKRSEYDKWLASRRDRRSSDRIIRAQGDVPFGTAGAPVG
                     PPQGSVMDFGRYMGWTLGQIKRHDPEFLEWLMRVPVGRQYRDEIGQMLRGPA"
     gene            2858..3544
                     /gene="rplU"
                     /locus_tag="VMZ33_07655"
     CDS             2858..3544
                     /gene="rplU"
                     /locus_tag="VMZ33_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HUR17146.1"
                     /translation="MYAVIETGGKQYRVELGSEVAIERLEGAPGDTIKFDRVLLVSDG
                     DSSSIGTPMVADALVTGEVLRQDRGEKVIVFKYRPKARHRAKKGHRQEQTVVRVADIQ
                     FNGRSAADEAQVTEQKEAKSKKAANAEAERKAAADAELAAKLAATAETEAAQATEADA
                     AEAKPAAKSTAKPKATSTKASTAVKASTTAKTSAKAAKPAKPKTEPKTATQSSGKTSP
                     AKPQSTPDED"
     gene            3550..3834
                     /gene="rpmA"
                     /locus_tag="VMZ33_07660"
     CDS             3550..3834
                     /gene="rpmA"
                     /locus_tag="VMZ33_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HUR17147.1"
                     /translation="MAHKKAGSSSKNGRDSAGQRLGVKAGDGQLVTSGSIIVRQRGMT
                     FLAGQGTDLGRDFTLFATTDGRVKFEHQRRDKKRVRVIAETATAPAGDAA"
     gene            3831..4043
                     /gene="rpmE"
                     /locus_tag="VMZ33_07665"
     CDS             3831..4043
                     /gene="rpmE"
                     /locus_tag="VMZ33_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HUR17148.1"
                     /translation="MKAEGHPKYQRATVTCGSCHTTFEVGSTQSTLRVDICSNCHPFF
                     TGKQTIIDTAGQVERFQKRLERSARL"
     gene            4139..5116
                     /locus_tag="VMZ33_07670"
     CDS             4139..5116
                     /locus_tag="VMZ33_07670"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HUR17149.1"
                     /translation="MARTDYGGQALLEGVLMRGRGAIGIALRHPDGSIVSTSEPLNSV
                     LHRSRFARAPFFRGLIVLYETLVVGTKWLMRSGSVQAESEGQSMGRGTIALTLLFTLT
                     LAIGLFVLLPLFLAGAAARATIGPGQFLLEHLIEGLIRVFIFVGYLLVISRSAEIRRV
                     FQYHGAEHMTIHALEREDPLDLEHVRRYPTAHPRCGTEFLVIFIVLSIIMFSLLAGQS
                     LVVGIIGRIALIPVIASLAYELLRFGAKHRHRWWIRWLYLPGIWLQAITTKQPDDSMI
                     EVAIASMREALGANGEAAPAGSADPARRPLELANAGDVATESAADHHAQ"
BASE COUNT          912 a         1745 c         1658 g          916 t
ORIGIN      
        1 ggttcgggcg cgctcgagga cgggatcatc aaagactcca cgctcgtgga gaccgctggc
       61 tacattgaga tcggcacgta caggtattac gactggaacc gacgtggttt cggaccgctg
      121 gacatctacg gcggcttcgc ccactcctcc gatacctact tctaccagct cgccggcacc
      181 ttgggcatcg atcggctcgg ccaatgggcc agcgaatggg gctttggcca gcgcacgggc
      241 atcgacctgc cagctgaggc gcgcggcatc gttcctacca acgagtggaa acagaatctc
      301 ttcagcgagc ccatctaccc gggcgaggtg taccaggcgg gcatcggcca gggctacgac
      361 gccgcgacgc cgctccaggt gctgaacgcc tataacgccc tggctaacgg cggcaaccta
      421 ctgaagccgc agatcgtgcg ccgcgtactg gcgcccgacg ggactgtcgt gagcgaattc
      481 cagcccgagc tcattcggga gctggatgtg agcaaggaca cactccggat catgcggctc
      541 gcggcgcgcg aggtggtcac cagccgccac accttcaacc tcgtggacct gccgttcgtc
      601 gtcgccggca agaccggcac cgccgagttc ggactgcgcg acgacaaggg gcgtctgccg
      661 tatcacacgt ggtttgcggc cttcgtgccc aacttcactg agacacagga gggcaatccg
      721 gaagcgactg actccgagct cgccatcctg gcgttcgcgt atgactcgaa cacaaaaggc
      781 aacgccgcgg tcgagatcgt caagtacttc cttcaggatc acttcgacgt cggcgtcgac
      841 ctgacccggc ccgacctcct ggccaagggc aacttctatg gcggccacta gggaacaacg
      901 gtcggacacc ggcgcgacgc cgggagccga ccgcttcgcg cgcagctcgg ctccgcaggt
      961 ccccgcgcga agggaaccga tgcgccgcgg cttgcgatga gcgtcatgcg aatcgagcgg
     1021 cggcgtgccg actcgctcct ggcccggccg gaatccagcg catggcagcg cttcgatttc
     1081 cagctcgtgt tctatgccgc cgcgctggcg accatcggcc ttctcgttgc ctggacgaac
     1141 agcgccggct cgccgctgag tccgggctcc gtcttcaccc ggggactgat gtggttctcg
     1201 attggcatcg tcgtgttcac cgcagcagtg gctttcgatt accgctggct gcgcgccttt
     1261 tcaccgctgc tctacctgat caacatcggg ttgctggtca tgaccctggc catcgggacg
     1321 ggcgtcggtg gcgtgcagcg ctgggtgtcg atcttcggac ttcaattcca gttctccgaa
     1381 atcagcaaga tcctgatgat tgccgtgctc gcggcatgga tgcacgcacg acgcgacaag
     1441 ctcggaagcc tgtcgacgat cctgggtgcc ggtctgctca cgttgccgcc attcgtgctg
     1501 gtgatgattg agccggacct gggcacgtcc ctcgtgttcc tcgcaatcct gttgggcacc
     1561 atgttcatga cgggcgcgag cctgcgctgg atgggcatcg gcctcgcaac gggcatcgcc
     1621 gcgctgcccg tcgcctggtc acagctggcc gactaccagc gccgtcgagt gctttcgttc
     1681 ctggatccca cggcagatac gaccggcgcc ggctggcaag cccttcaatc tcagatagcg
     1741 gtcggatcgg gcgggatatt cggtaagggg ctgaccaatg ggagccagtc gagctccgac
     1801 ttcctgcccg tccaatccac cgacttcgct ttcgccgtgc tgctcgagga gctcggcttc
     1861 gtcgggggca tggtcgtctt cctgctgttt gtcggcttgg tctggcgggt cctggtcatc
     1921 ggctggcgca gcgagtcggc ctttggcgtc gccttctgcg cgggcgtggc atcgatgatc
     1981 gtgttccagc tgttggtcaa tgccgggatg gtggcgggga tcatgccggt cactgggatc
     2041 ccactgccgt tcatcacaca tggcggcgct tcgctgacgt ccatcgcgat tgcactcggc
     2101 ctggtgcaga gcatcaacat cagagagcca cgcggctcgc cagtggacta ggccggtcct
     2161 cggagcatct gtccgatttc gtccctgtat tgccggccga cagggacgcg catcagccac
     2221 tcgaggaatt cgggatcgtg tcgcttgatc tggcccagcg tccagcccat atagcgcccg
     2281 aaatccatca cgctgccctg cggcggccct accggcgcac ctgctgtgcc gaatggcacg
     2341 tcgccctgtg ctcggatgat ccggtcagac gagcgccgat cacgacggct ggccagccac
     2401 ttgtcgtact cgctccgctt ctgcgcatca gacagaacct cgtgtgcgac attgatttcg
     2461 gccatccgcc gggcggcttc cgggctggga tcgacatcgg cgtgaaaacg ctgtgcaagc
     2521 tttcggtaaa cgacgccgat gatttcggcg tcggccgtct cagccagcat gagcgtcttg
     2581 tagtacgtat gacgcgggtc ccgtgtagtc atgttgggct gcatgctggt cgacgtacca
     2641 cgggaccgaa attgccagtt cgtcccttgg cgctgggtac ggcggtccta cccgatcgcc
     2701 cactcgggtc gaaagcaggg gccgtggtat ccttcgcgat cgcgctcgat ggcaaggtcc
     2761 gctcgactgg cccagcgcag acgctccacc cagagagact ccacatagaa ccagtcacgc
     2821 ccaacacgcg cgctcggcgg atcgcgcacg gagaaccatg tacgcagtta tcgaaaccgg
     2881 tggaaagcag tatcgagttg agctcggtag cgaggtcgca atcgagcgtc tcgagggcgc
     2941 acccggtgac acgatcaagt tcgatcgagt cctcctcgtg tctgacggcg actcgtcatc
     3001 aatcggcacg cccatggttg ccgacgctct cgtaaccggc gaggtcctgc gccaggatcg
     3061 tggcgagaag gtgatcgtct tcaagtaccg accgaaggcg cgccaccgcg cgaagaaggg
     3121 ccaccgccag gagcagaccg tcgtccgcgt cgcggatatt caattcaacg gtcgcagcgc
     3181 cgccgacgag gcccaggtca ccgagcaaaa ggaagccaag tcgaagaagg ccgcgaacgc
     3241 agaggcagag cgcaaggccg ccgccgatgc tgagctcgcc gccaagctcg ccgcgacagc
     3301 cgagactgag gcggcccagg ccactgaggc cgacgcggcc gaagccaagc ccgccgctaa
     3361 gtcgactgca aagcccaagg cgacgtccac gaaggcgtcg accgcagtga aggcatcgac
     3421 caccgcaaag acgtcagcca aggccgccaa gcccgccaag cccaagaccg agcccaagac
     3481 cgccacgcag tcttccggca agacgtcacc ggctaagccc caaagcacac ctgacgagga
     3541 ctaaaagaga tggcacacaa gaaggctggc tccagctcta agaacggccg cgacagcgcc
     3601 ggccaacgac tcggcgtcaa ggccggggac ggccagcttg tcacgagcgg gtcgatcatc
     3661 gtgcgccagc gcggcatgac gttcctcgcc ggtcagggca cggatctggg tcgtgatttc
     3721 accttgttcg cgaccacgga cggccgcgtc aagttcgagc accagcggcg cgacaagaag
     3781 cgcgtccgcg tcatcgccga gaccgccacg gctccggcag gagatgccgc atgaaggccg
     3841 agggtcatcc caagtaccag cgcgcgaccg tcacgtgcgg ctcgtgccac acgaccttcg
     3901 aggtcggatc gacacagtcc acgttgcgcg tggacatctg ctcgaactgc catccgttct
     3961 tcactggcaa gcagacgatc atcgataccg ccggacaggt tgagcgcttc cagaagcgcc
     4021 tcgaacgctc ggcgcgtctc taatccccgc aaggggaggg gacgacacgt cccggcgcgg
     4081 cgggtcggca tggagggcgc agcagcggcc tggctgccgg ctgcggaggt agacctaatt
     4141 ggcgcgaact gattacggcg gccaggcact gctcgagggc gttctcatgc gtggtcgcgg
     4201 tgcgatcggc atcgccctgc ggcaccccga cggctcgatc gtgtccacgt cggagccact
     4261 caactccgtc ctgcatcgca gccggttcgc gcgcgccccg ttcttccgcg ggctcatcgt
     4321 cctgtacgag actctcgtcg tcggtaccaa gtggctgatg cgctcgggct cggtccaggc
     4381 cgagagcgag ggccaatcga tgggccgcgg caccatcgcg ctgaccttgc tgttcaccct
     4441 cacgctggcg atcggcctgt tcgtgctgct gccgctgttt ctcgctggcg cggctgcgcg
     4501 cgcgaccatc ggcccgggtc agttcctgct cgaacacttg atcgaggggc tgatccgcgt
     4561 ctttatcttc gtcggctacc tgctcgtgat cagccgctcg gcggagattc gccgcgtctt
     4621 ccagtaccac ggcgccgagc acatgacgat ccacgcgctg gagcgcgagg acccgctcga
     4681 cctcgagcac gttcgcagat atccgaccgc ccacccgcgc tgcggcaccg agttcctggt
     4741 gattttcatc gtcctgagca tcatcatgtt cagcctgctg gcgggacaga gcctggtggt
     4801 cgggatcatc ggccgcatag cactcatccc ggtgatcgcg tcactggcct acgagctgct
     4861 gcggttcggt gcgaagcatc gccaccggtg gtggatccgc tggctgtacc tgcccgggat
     4921 ctggctccag gcgatcacca cgaaacagcc tgacgactcc atgatcgagg tcgccatcgc
     4981 gtcaatgcgc gaggcacttg gcgccaacgg tgaggcggct cctgccggca gcgccgatcc
     5041 ggcgcggcgg ccgctcgagc ttgcgaacgc gggagacgtt gccaccgagt cggcggccga
     5101 ccaccacgcg caatgacact cgagctgcgc ctcgacgagc tcgagcagcg gctcctggag
     5161 atcgaggccg agtggtccaa ccctgaggtt tcggccgatc acgaccgctc ccggctcctt
     5221 ggccaggagc a
//
LOCUS       DATLCO010000333         3190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6364799, whole genome shotgun sequence.
ACCESSION   DATLCO010000333 DATLCO010000000
VERSION     DATLCO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6364799"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VMZ33_07675"
     CDS             complement(<1..375)
                     /locus_tag="VMZ33_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17150.1"
                     /translation="MTAVLEAPTRVAREVTVGRDVALLRGRTGYVVGGIALVAFGAIF
                     AAVKRRRSDEVDVAITLRLQARQYPALAKAMELASWPGFPPQSRVIPPLVIATMFVMR
                     LRIEAGFQTLAWGTALMSTVVKA"
     gene            complement(452..1285)
                     /locus_tag="VMZ33_07680"
     CDS             complement(452..1285)
                     /locus_tag="VMZ33_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR17151.1"
                     /translation="MRVCLWGTRGSIASAGPDTVAYGGNTSCAEVEARDGTILILDAG
                     TGIRRVGDTYKEPRRLDILLTHLHMDHIQGLGFFAPFFRPDFEVHLWGPPSTTQDLRT
                     RLTRYLSPPLFPVRLRDVAAHLELHDVPMEPFRIGGFEIQAQVVIHPGQTIGYRISDG
                     VATVAYIPDHEPALGLNGDLADPQWTSGHDLAAGADILIHDAQYTTEEYDARVGWGHS
                     RIGDAVALARMAGVQRLVTFHHDPAHDDSELDQMIDDARALAGDGLEVLPGREGATLH
                     A"
     gene            complement(1300..1713)
                     /locus_tag="VMZ33_07685"
     CDS             complement(1300..1713)
                     /locus_tag="VMZ33_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUR17152.1"
                     /translation="MAQRDVGLILMRQLASGLAVPMLLADDAGDLLFFNEPAEVLLGQ
                     RFDDVGEMPLDRRRRIFSFRDVEGKPLPDDQPPLVVALRERRPVHRRVWMRGFDGEDR
                     EIEVTAFPLLGAGGHLIGGVAMFWQRRQPGSMNGA"
     gene            complement(1792..2397)
                     /locus_tag="VMZ33_07690"
     CDS             complement(1792..2397)
                     /locus_tag="VMZ33_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HUR17153.1"
                     /translation="MFRRVTVGGLLLCVATALLPASTVASASEQERVVSIATAQLSKP
                     FRLGAEGPRRFDCSGLIYFTFKNAGLYERFGTRRLLARNYYTWGRERGLLGTTNPRVG
                     DLVLWTHSGTIVHMGMYIGVDNRGRARALSALTTGVSRHSIGGISVRFLTYVHVGLDG
                     TPDPDPSPSGSPPPTVSPSATPVEPGATPSLAPTPLPTPTL"
     gene            2629..2856
                     /locus_tag="VMZ33_07695"
     CDS             2629..2856
                     /locus_tag="VMZ33_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17154.1"
                     /translation="MTGNLCAVDERQTSDDNLSCTLCGRPLGYSTDDQPDWPTGPMCG
                     DCYQAREMDNDIWAAELDEDDEDDEADAENL"
     gene            2919..>3190
                     /locus_tag="VMZ33_07700"
     CDS             2919..>3190
                     /locus_tag="VMZ33_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17155.1"
                     /translation="MSKRGARVLGIEQFEPAHELGSSGGLSRIIRLAYFEHPDYVPML
                     KAAWALWPQIEAEAGVELLKVTGGLYVGREGSAVLDGSRRSAQEHGL"
BASE COUNT          573 a         1055 c         1070 g          492 t
ORIGIN      
        1 cgccttgact accgtcgaca tcagcgccgt gccccaagcg agggtctgaa agccggcctc
       61 gatcctcaac cgcatgacaa acatcgtcgc gatcacgagc ggcgggatca cgcgactctg
      121 cggcggaaag cccggccatg aggcaagctc catcgccttt gcaagcgctg gatactggcg
      181 cgcctggagg cgaagggtga tcgcgacatc gacctcgtcg gacctgcgcc tctttacggc
      241 ggcgaagatc gcaccgaaag ccaccagggc gataccaccc acgacgtagc ctgtccgccc
      301 acgcaggagc gccacgtcgc ggccgacggt tacctcacgt gcgacgcgtg tcggcgcctc
      361 aagaactgca gtcacgtgtc gattagacct gcagcgaagc cattcggcca gcccgcggag
      421 ccgttgcgac ctatcaaccc cgtcactgga cctacgcgtg gagggtcgcg ccctcgcgtc
      481 cgggcagaac ttccaggccg tcgcccgcca gagcccgcgc gtcgtcgatc atctggtcga
      541 gctcgctgtc gtcatgggcc gggtcgtggt ggaacgtgac gagcctttgc acaccggcca
      601 tcctggccag cgcgacggcg tcaccaattc tggagtggcc ccaacccacc cgcgcgtcgt
      661 actcctccgt cgtgtattgg gcatcgtgta tcaggatgtc ggcaccggcg gccaggtcgt
      721 ggccggaggt ccattgggga tcggccagat caccgttcaa gcccagggcc ggctcgtggt
      781 cggggatgta agcaaccgtt gcgacgccgt ctgagatccg gtagccgatt gtttggccgg
      841 gatggatgac cacctgcgcc tggatttcga acccgccgat gcgaaagggc tccatcggca
      901 cgtcgtgcag ctcgaggtgc gcggccacgt cgcgtagccg gaccgggaag agcggcggcg
      961 acaggtagcg agtcagccgc gtgcgcaggt cctgcgtcgt cgacggcgga ccccacagat
     1021 ggacttcgaa gtccggccgg aagaacggcg caaaaaaccc gaggccctga atgtgatcca
     1081 tgtgaaggtg agtgagcagg atgtcgagcc gccgcggctc cttgtaggtg tctcccacgc
     1141 ggcgaatgcc ggtgcctgcg tcaaggatga ggattgttcc gtcgcgcgcc tcgacctccg
     1201 cgcacgaggt gttgccgccg tacgcgacgg tgtcagggcc ggctgaagcg attgagccgc
     1261 gcgtgcccca taggcatacg cgcatggatg gatccgcggt cacgccccgt tcatggaccc
     1321 cggctgccgg cgctgccaga acatggccac gccaccaatg agatggccgc ccgcgccgag
     1381 cagtggaaag gcggttacct cgatctcgcg gtcctcgccg tcaaaaccgc gcatccagac
     1441 ccgacggtgt acggggcgcc gctcgcgcag cgccacgacg agcggcggct gatcgtcagg
     1501 gagcggtttg ccctcgacgt cgcggaagct gaagatccgc cgccgtcggt cgagcggcat
     1561 ttcgcccacg tcgtcgaaac gttgaccgag cagaacctcc gccggctcgt tgaagaacag
     1621 gaggtcgccc gcgtcatcgg cgagcaacat cggcacggcc agcccggacg cgagctggcg
     1681 catgaggatc aggccgacgt cgcgctgtgc catgtgccgg gattgtcgct aatcagcgac
     1741 cgtccgcaac aacgaactgg cggcgcccac agcccttcac ggctacaagt cctagagcgt
     1801 cggcgtcggg aggggtgtcg gggccaggct tggcgttgcg cccggttcga cgggagtggc
     1861 gctcggactg acggtcggtg gcggcgaccc cgacggagac ggatcggggt ccggcgtacc
     1921 gtcgagcccg acatgcacgt acgtcaggaa acgtacggat atccctccga tgctgtgccg
     1981 gctgacgccc gtcgtcaggg cgctgagcgc ccgggcgcgc cctcggttgt cgacgcctat
     2041 gtacatgccc atgtgcacga tcgtccccga atgcgtccac agcacgagat cgccgacacg
     2101 cggattggtc gtgcccaaga ggccgcgttc cctcccccac gtgtagtaat tgcgcgccaa
     2161 caaccgccgg gtgccaaagc gctcgtacaa gccggcgttc ttgaacgtga agtagatgag
     2221 cccggagcag tcgaaccgac gcgggccctc ggcgccgagc cggaacggct tgctgagctg
     2281 cgctgtcgcg atcgacacga cgcgctcctg ctcgctggct gaagcgacgg tcgatgctgg
     2341 caacagcgcc gtcgcgacgc aaagcagcag cccgccgacg gtgacacgtc ggaacacggt
     2401 gaacccctct taataacccg cgtgctgggg caagcgggct ggcatgcacc ttacgccacg
     2461 tgttgccaaa aggcgacacc caaaaagaga agcccccgcg aacggaggct tctcgctgga
     2521 cgggtcttgg gtctggggcg cctatcgacc agtgctgggg caccggttac agggctcgtc
     2581 aatcgtaggc gacagccgcg tgccacacct catccaaacg cagtatttgt gaccggtaac
     2641 ctctgcgccg tggacgagcg ccagacatcc gacgacaacc tcagctgcac cttgtgcggc
     2701 cgaccactcg gctacagcac tgacgatcag cccgactggc cgaccggccc catgtgcggc
     2761 gactgctacc aagcgcgcga gatggacaac gacatctggg ccgccgagct cgacgaggac
     2821 gacgaggacg acgaagcaga cgcggagaac ctgtgacccg gacgtacgac gccattgtcc
     2881 tggggctggg tgctatgggc agctcggctg cctaccaaat gtccaagcgt ggcgcgcgcg
     2941 tgctgggcat cgagcagttc gagcccgccc acgagctggg ctcgtccggt ggcctgtcgc
     3001 gcatcatccg gctggcgtac ttcgagcatc ccgactacgt gcccatgctc aaggcggcgt
     3061 gggcgctgtg gccgcagatc gaggccgaag caggtgtgga gctgctgaag gtgaccggag
     3121 gcctatatgt cgggcgtgag ggcagcgccg ttctcgacgg atcgcggcgc agcgcgcagg
     3181 agcatgggct
//
LOCUS       DATLCO010000334        11386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7177165, whole genome shotgun sequence.
ACCESSION   DATLCO010000334 DATLCO010000000
VERSION     DATLCO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7177165"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..625
                     /locus_tag="VMZ33_07705"
     CDS             395..625
                     /locus_tag="VMZ33_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HUR17156.1"
                     /translation="MSRLDRITVDPAMCHGKPTVRGLRYPVQSLLELLASGMTIDEIL
                     DDYPDLEREDLLAALEFGARTAGNRHVPLGAS"
     gene            622..981
                     /locus_tag="VMZ33_07710"
     CDS             622..981
                     /locus_tag="VMZ33_07710"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HUR17157.1"
                     /translation="MKFLIDAQLPALLARRLIEVGHDAIHTLDLPGGNRTPDDEIAHL
                     ADADDRVVVSKDGDFRDSHLLRQTPKKLLIVATGNITNRELIALFEEHLGAIVAALDG
                     ADLVELAAAALTVHDRD"
     gene            complement(1197..1442)
                     /locus_tag="VMZ33_07715"
     CDS             complement(1197..1442)
                     /locus_tag="VMZ33_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17158.1"
                     /translation="MNSTQASTSTTRRSLAPAALGLGLASVALITVVAFIAMALGLAA
                     IVVGVASSARSSRGRVVALLGAIAGAGSILFFILHVM"
     gene            1562..2734
                     /locus_tag="VMZ33_07720"
     CDS             1562..2734
                     /locus_tag="VMZ33_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HUR17159.1"
                     /translation="MVERWIRGVHWTDVGLAIGLTAVGVSATLTAEDYTGGRARLTAA
                     LLLQTLPLVWRRSAPLRAVSLSALGVAIEVAGAAAWGDVAGFFGYLVLAYSVPRWGQR
                     RDRLVAGAILGAGFVVHLTSQPHDGVSDVIGSMVSASIMTAAAWALGTAMRSRSERVH
                     ALEEDVTEMERRWADERRAVIEEERRRIARDLHDIVGHALAAISLSAGGAEQCLDASE
                     GDSPGIVETRLALNSIRTTSRDAAADVRRLVGLLRTENDVNQVSPQPTLAGVPQLVGR
                     ARSAGLDVELADGDNLAGLPLGVQLALYRVVQEGLTNVTKHAPGSSVRVCIARRNGGV
                     VAEIENSPPSGPPTPAVDRGHGLVGLAERVALYDGDLRGEPTTEGGFLLATRIPVS"
     gene            2734..3363
                     /locus_tag="VMZ33_07725"
     CDS             2734..3363
                     /locus_tag="VMZ33_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR17160.1"
                     /translation="MNLDAPEVRVVIADDEAIVRHGLRLILDHARGISVVAEAANGRE
                     AIEAAATHRPDVVLMDVRMPVLDGIEATRRITTGAAQVLILTTYDLDENLYLALRAGA
                     CGFLLKTSAPEDLVHAVRVVARGDALVEPKITRRLLAEFLERPPPLGAAVPTLSHRYP
                     NASARCYASSPPAGRTPRSAPSFSSAKARRRPTWRASSQSSACATASRL"
     gene            complement(3860..4465)
                     /locus_tag="VMZ33_07730"
     CDS             complement(3860..4465)
                     /locus_tag="VMZ33_07730"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR17161.1"
                     /translation="MSTVTVRRATLGDTPHMAKALGRAFADDPLFDWLVPDAEKRHEM
                     LPDWFKLGFDKLFKRRETYTTDDHAGAAIWAAPGEWKTKPLEMLSMIPGSIRIVGLGP
                     MRRLITTFNAIEKQHPEEPHWYLEGLGTDPPKQRQGVGAALMKPVLERCDAEGVPAYL
                     ETQKERNVPYYRSHGFEVTKEMDLPKGGPHLWLMWRDPRAS"
     gene            complement(4677..5789)
                     /locus_tag="VMZ33_07735"
     CDS             complement(4677..5789)
                     /locus_tag="VMZ33_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR17162.1"
                     /translation="MVARLGELAAAVAEHRSKFDSDGRLPDDLFQELATLGLFRLLLP
                     SSLGGRGLSALEFMDVVEAAAALDGTIGWLASNGGGMARVGAYLPAESAREIFDDPLA
                     FVVSSTAALGRAVRVRGGYSVTGRWPFGSGSPHGTWFSPICAVEGDEQPTGEMIFVLA
                     PRNDVLLHDNWQVSGLCATGSVDFELADVFVPDRLAHAFQPEPTQPGTLYRLPTRSIY
                     SWTVATVPLGISAGAINDFAVIASSGRGASVPLGERELIQSQLGQIQARTAASRAYLR
                     HTMRTLLEEVEAGNGLDASRVDFRLACTFASQSALWAIDLVTEMVGTVAIQRSSPLER
                     RERDARAAAKHIAMSPAAYITGGKLRLGHDLSKVNF"
     gene            complement(6054..6278)
                     /locus_tag="VMZ33_07740"
     CDS             complement(6054..6278)
                     /locus_tag="VMZ33_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17163.1"
                     /translation="MAVDLTSTGMRRGYAQARVTASGFLLMVVVPILIARGAPAAQWL
                     MVIGPLVSLATGVIHLRRTSPEGRRIRAGA"
     gene            6469..6993
                     /locus_tag="VMZ33_07745"
     CDS             6469..6993
                     /locus_tag="VMZ33_07745"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR17164.1"
                     /translation="MHINGPTGVGKSAIAEGLVEKRPLALCLDIDALRVRLGQWRELN
                     DSKQIARNLGFALVQRHLEAGHDVVLPQLIVREDVVDDLEKLAIEAGASFVEVILRAE
                     PRELAARVERSRAGQVGEHPRDQFTSAELAQQTEYALEVLAQMAARRMNVTVVDVSGM
                     SLAQATAAVVAVLV"
     gene            complement(7376..7876)
                     /locus_tag="VMZ33_07750"
     CDS             complement(7376..7876)
                     /locus_tag="VMZ33_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17165.1"
                     /translation="MDAYVVGLRAVHIVAAASWVGASLSFAFFIEPVANRLGPSASQF
                     MIELTERRKFPVAIAVVSVLAIVAGMLLYWRDSSGLEWAWISTRTGAMFTAGAVAALS
                     AWLVGFLILRPGIARTAALAAAAPTDPTAQDELARLGHRLRTASLVNAWLIILATLAM
                     ATARYV"
     gene            8004..9188
                     /locus_tag="VMZ33_07755"
     CDS             8004..9188
                     /locus_tag="VMZ33_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUR17166.1"
                     /translation="MRPPDLHRLRAGLSAVVGRQLGFIEFAAEATELLAAAVPFDRSC
                     WHTVDPGTVLFTGSVNQGISCSGRWLADHEYVIDDVNQWSTLARTGQLAGRSSAATSG
                     DLNRSARHRSTASWGIGDELRVSFVVDGVYWGAAGFLRDDDRPWFTEDEEQEMRAVID
                     LLASGCRRALVGTGHLVRDAPNVKGPAVMILADNGEAEVVTPAAALWVNDLLEEPRPR
                     TPAQSKVVQAVAAQSRARGDGSDPIAAPSRARVRTRSGEWLVVYATTLSGETGARTAI
                     VIQPAVPAEVAPLLAVAYGLTNREKDVSRLCLQGRSSKEIALALGITVYTVQDHLKSI
                     FEKTATRTRNELVGRVFLSHYTTRWEEHADVPPGWTANIAAEAATPRTPSADDANSGG
                     RR"
     gene            9456..10265
                     /locus_tag="VMZ33_07760"
     CDS             9456..10265
                     /locus_tag="VMZ33_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR17167.1"
                     /translation="MGRFGAIRESVVTVEDATTCFTEWGDPDGAVVVGWPGNPGSRFG
                     LGWARLYASECGVRLFVFDRPGFGRSSPDPSRTVSSTARRICEAVASLGIESFGVLGN
                     SSGGPYALACGAVAPGSVTSIAVIAGGGRMDEAGSHSGMSIENDRFWSLASQGPSTTT
                     PDFEQLLRNAGANAKGLHKERVADIAEAGRQGAEHLALDAHVITARWDFDESDITMPV
                     DLWHGAADDDIPLEQAWRLADRLPRATLHVWPRHGHDMPPEAMTAVYALLR"
     gene            10443..11123
                     /locus_tag="VMZ33_07765"
     CDS             10443..11123
                     /locus_tag="VMZ33_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HUR17168.1"
                     /translation="MATTLSNLIDEVEQRSPSNNPLDLLATAAATVEEVTDAADALLS
                     HFVDRTRRAGHSWTEIGAALGVTKQAVQKRFTGDEKRNPRGWERFTDRARAVASKHAF
                     AASEELGNNYVGTEHLLLAMWGEPEGLAALALVDAGLTRETVIEAIDARIPRGAQGRG
                     GFTPRAWVAIENSTKVAIELGHNYVGTEHLLLSLMTGVGGVADEILGEKGVKRDKVEA
                     FVLAALKS"
BASE COUNT         1947 a         3776 c         3772 g         1891 t
ORIGIN      
        1 ggagcgcatg aagtcgaggt ctccgccgga gcggatcgag tcgagtagat cggacagggc
       61 agactggtcg agggccatcg tggcgttctc cttcgtgatc ccggctagga acacgtcgaa
      121 gatcacgcgg tggcccacct atgtggtgga cccctccaaa accggcctct cagatcgccg
      181 cagagccctt caacgagctc gacccgatag gagacaccgg ccagatcagg ccctcaaatc
      241 ccaccactcg aggggacact cctcgagtcg ggctcgtccg ctctcgtctg cgcccgtcgg
      301 tccgagttgc cttgacaaat ccgtgacaag aacccggacg cttgggtgga cacgcggggg
      361 tcaaattggc gccggtgccg gtaccatcag cgccgtgtca cgtctcgatc ggatcaccgt
      421 tgacccggcc atgtgccacg ggaagccgac agtccgcggc ctgcgctatc cggtgcagag
      481 cctcctcgaa ttgctcgcca gcggcatgac gatcgacgag atcctcgatg attaccccga
      541 ccttgaacgc gaggatctcc tcgctgccct tgagttcggc gcgaggacag ccggaaatcg
      601 ccatgtcccc ctcggcgctt cgtgaagttt ctgatcgacg cgcagctccc cgccctcctc
      661 gcccgtcgcc tgatcgaggt gggtcacgat gcgattcaca cgctcgatct cccaggcggg
      721 aaccggacgc ccgacgatga gatcgcgcac ctcgccgatg ccgacgaccg agtcgtcgtc
      781 tcgaaggacg gcgacttccg tgacagtcat ctactgcgcc agacgcccaa gaagctgttg
      841 atcgtcgcca ctggaaacat caccaaccgg gaactgatcg cgctcttcga ggagcacctc
      901 ggggcgatcg tcgccgcgct cgacggcgcg gatcttgtcg agctggccgc cgcggcgctg
      961 acagttcacg acagggacta acccggcaag gcggccgcag agaggtggcc tattgggaca
     1021 gtccgccgcg attgctctga tcgccctcga ccggcccatg tgggctaccc cgaagaacct
     1081 cgggcgatgc gcacaatgtg gcgcgtcgcc ggctcgtgat cgccgggtag cggctgcctg
     1141 cgttcggcgg tttggtgttg cttccggttc ggcgaggcgt ggcgacaaca gcggcctcac
     1201 atcacgtgga ggatgaagaa caggatcgag ccggcgccag cgatcgcgcc caggagcgca
     1261 acgacccggc ctcgggagga ccgggctgac gatgcgacac cgacgacgat cgcggcgagg
     1321 ccgagcgcca tggcaatgaa ggcgacgaca gtgatcagcg ctacggaagc caggccgagg
     1381 ccgagggccg cgggagcgag gctccgcctg gtggtggacg tggaagcttg ggttgagttc
     1441 atgacaacaa ggaacatcgt cggccttgtc cggcgcatcc cccccgaggc gggttcggat
     1501 catccctccg gcagaggcaa acctgtccca cggcgggtgc gcatcccagt acgttccgtg
     1561 tgtggtcgaa cgatggatcc gtggtgtcca ctggacggac gtcgggctcg ccattgggct
     1621 caccgccgtc ggtgtcagcg ccacgctcac ggccgaggac tacacgggcg gacgcgctcg
     1681 gctcacggcg gcgttgctgc tccagacgct tccgttggtg tggcggcgat cagctccgct
     1741 ccgggcggtc tcgctgtcgg cgctcggcgt cgccatcgag gtggcaggtg cggccgcgtg
     1801 gggtgacgtc gccggcttct tcgggtacct cgtcctggcc tactccgtgc ctcgatgggg
     1861 gcaacggcgt gaccgcctcg tcgcgggtgc gatccttggc gcgggattcg tcgtccacct
     1921 gacctcccaa ccccacgacg gagtctcgga cgtcatcggc tcgatggtgt cggcttcgat
     1981 catgaccgca gccgcgtggg cgctcggtac agcgatgcgc tcccgctccg agcgcgtaca
     2041 cgcgctcgag gaggacgtga ccgaaatgga gcggcgatgg gccgacgagc gtcgcgccgt
     2101 catcgaggag gagcgtcggc gaatcgcccg cgacctgcac gacatcgttg gtcacgcatt
     2161 ggcggcgatc tcgctttcgg cgggaggcgc ggagcagtgc ctcgacgcat cagaaggcga
     2221 cagtcctggc atcgtcgaga cccggctggc gctcaactcg atccgcacga cgagccgcga
     2281 cgcggcggcc gacgttcgcc ggctcgtcgg cctgctgcgc acggagaacg acgtcaacca
     2341 ggtctcgccc cagccgacac ttgccggcgt tccgcaactc gttggtcggg cccgctcggc
     2401 tggtctcgac gtcgagctcg ccgatggaga caacctcgcc ggacttcccc ttggcgtcca
     2461 gctggcgttg taccgcgtcg tccaagaagg acttaccaac gtgaccaagc acgccccggg
     2521 ctcatctgtc cgagtttgca tcgctcggcg aaacggcggc gtcgtcgcgg agatcgagaa
     2581 ctcaccaccc agcggcccgc caacgccagc cgtcgatcgc gggcacggcc tcgtcggact
     2641 cgcggagcgg gtcgcgctat acgacggcga cttgcggggc gagccgacga ctgagggtgg
     2701 ctttcttctc gccacgcgta tcccggtctc gtgatgaacc tcgatgcgcc cgaggtccgg
     2761 gtcgtgatcg ccgacgacga agcgatcgtc cgtcacgggc tgcgcctcat cctcgatcat
     2821 gcccgcggca tctccgtcgt cgctgaggcg gccaacggac gcgaggcaat cgaggccgca
     2881 gccacccacc gacccgacgt cgtcttgatg gatgtccgca tgcccgtgct cgacggcatt
     2941 gaagcgacaa ggcgtatcac gaccggcgcc gcgcaggtgc tgatcctcac gacctacgat
     3001 ctggacgaga acctgtacct ggccctgcga gctggcgcgt gtgggtttct actcaaaacg
     3061 tcggccccgg aggacctcgt acacgcggtg cgtgtcgtcg cccgcggcga tgcgttggtc
     3121 gagccaaaga ttacgcgtcg actcttggcc gagtttctag aacgaccccc accgctcggc
     3181 gccgcggttc ccacgctgtc gcatcgctat ccgaacgcga gcgcgaggtg ctacgcctcc
     3241 tcgccgccgg ccggtcgaac gccgagatcg gcgccgagct tttcgtcagc gaaggcacgg
     3301 cgaagaccca cgtggcgagc atcctctcaa agctcggcgt gcgcgaccgc gtccaggctg
     3361 tgatcgccgc ctacgagtgc ggcttcgtcc gcccgggcag agcgcccacg gattgaagga
     3421 tgtcgacgcg cgcgagatcc tcgagcgtcc tgtccccgga tgaccggtag ccggacgggt
     3481 gcggcgctac cgccgggagc gcccactttc gaccggcgag tcgccgtcgt ttggagacag
     3541 tcgaattgtg ttggcgtccg acgccacgaa cggcccttcg tggcgatgcc ccacgtcgat
     3601 atgtcggcac atttggggac acttgtgacg cagttaccgc agcccgtcgg gctcccgcgg
     3661 cctgcgggac tggcccttcg gggatgcgcc gcccacgacc gttcggggac accgacggcc
     3721 ccgttcctca cccgtttccg tcattcaggt cgcgcccagg gctactggct gttcatctcg
     3781 atcgccgagg acgatccact cgtctgcagc cggcagcgtc gaacgtcggc gatgtcgggc
     3841 agcctgtcgg gcgagtgacc tacgacgctc gggggtcccg ccacatgagc cagaggtggg
     3901 gaccgccttt cggcaggtcc atctccttgg tgacctcgaa gccgtgcgag cggtagtacg
     3961 gcacgttgcg ttccttctgc gtttccaagt atgcgggcac gccctcggcg tcgcaccgct
     4021 cgagcacagg cttcatcaac gctgcgccca cgccctggcg ttgcttgggc gggtcggtgc
     4081 caagaccctc gagataccag tgcggctctt cggggtgctg cttctctatg gcgttgaatg
     4141 tggtgatcaa gcggcgcatg gggcccaagc cgacgatgcg gatgctgccc gggatcatcg
     4201 acagcatctc gagcggcttg gtcttccact cacccggtgc ggcccagatg gcggcgcccg
     4261 catggtcgtc ggtggtgtac gtctcgcgcc gcttgaacag cttgtcgaac ccgagcttga
     4321 accagtccgg gagcatctcg tggcgcttct cggcgtcggg cacgagccag tcgaagaggg
     4381 gatcatcggc gaatgcccgc cccaatgctt tcgccatatg gggcgtgtcg ccgagcgtcg
     4441 cgcgtcggac cgtgactgtg ctcattgggc gcagtcaacg ggggtttcgt gatcctgtca
     4501 atgccttcct cccggcccgg cacctcgggt gccttccctg cacgacggcg ttgccgggca
     4561 gctagggcct ggcgctcacg gcgcgtttcg agagcgaacc gccgtcattc gggggcaccc
     4621 cactcgcgat gtcatcgcgc gcctgccatc ggcccatgtg gggctcaccg tctgattcag
     4681 aagttgacct ttgataggtc gtgtccgagg cggagcttgc cgccggtgat ataggccgcc
     4741 gggctcatcg cgatgtgttt cgcagccgcg cgcgcgtctc tctcccgcct ctcgagcggc
     4801 gacgaccgct gtatggcaac cgttccgacc atctcggtca ccagatcgat tgcccagagg
     4861 gctgactggc tggcgaatgt gcaagcaagt cggaagtcga cccggctcgc gtcgagacca
     4921 ttgccggcct cgacctcttc gagcagcgtg cgcatcgtgt ggcgaaggta cgcccggctg
     4981 gcagcggtgc gtgcctggat ctggccaagc tgcgactgaa tcaactcgcg ctccccgagc
     5041 gggacgctgg cgccgcgccc gctggaagcg atcactgcga agtcgttgat cgcgccggct
     5101 gagatcccga gcggcacggt ggcgaccgtc catgagtaga tggagcgggt gggtaaccgg
     5161 tagagcgtgc cgggttgcgt gggctctggc tggaacgcat gcgcaaggcg gtcgggtacg
     5221 aagacatcgg ctaactcgaa gtcgacgctc ccggtcgcac agagacccga gacctgccag
     5281 ttgtcgtgga gcaggacatc gttacgcggt gcaagaacga agatcatttc gccggtcggt
     5341 tgctcgtcgc cctcgaccgc acagatcggg ctgaaccacg taccgtgcgg tgagccgctt
     5401 ccgaacggcc accgaccggt cacggagtaa ccgccgcgaa cgcgaaccgc acgccctaga
     5461 gcagcagtcg acgagaccac gaaggccaag ggatcgtcaa agatctccct cgcgctttct
     5521 gccggcaagt acgcccccac gcgagccatg ccgcccccat tgctggccag ccaaccgatc
     5581 gtgccgtcca gagccgccgc cgcctcgacg acatccatga actcgagagc ggacaggccg
     5641 cgcccaccca gcgacgacgg aagaagcagg cggaacaggc ctagcgtggc gagctcctga
     5701 aacaggtcat caggcaatcg accgtcggag tcgaacttgc tccgatgctc cgctactgcc
     5761 gctgcgagct caccgagtct tgcaaccacc tctcgagctg cacccgagct tcgactctcc
     5821 acgaacgatg tcatggccgc ctgcccccct aacgctgttc gccccaacgt taggcacgcc
     5881 gcacactcgg cgactccgtc gtgggttcct cgaagatcgc gaccgggtcg ttggcgtcca
     5941 ggcacgcgac gttgcggagt atcccagatg gccggctgag tgacgcacct cggcgcgcgc
     6001 catttaccga ggctgtctcg cgccggcgct tccgatcgac ggctacgcgc ttgtcacgcg
     6061 cccgcccgaa tgcgccggcc ttcgggtgag gttcgacgca ggtgaatcac gcccgttgcg
     6121 agactcacca gcgggccgat caccatcagc cactgagcgg ccggcgcgcc gcgcgcgata
     6181 aggatcggta cgacgaccat taacaggaat ccgctcgcgg taacgcgtgc ttgggcatat
     6241 ccgcgcctca tgccggtgga cgttagatct acggccacca cagtgcctgc agcggcggct
     6301 cgaacgcgac aggtcgaagg ctgttcgcgc cgctcggcga aggatcaggc gacccgaacg
     6361 tgcgtcccat cagacgcgcc cacaagactg caaggcgact gccccagatg gccggttgcg
     6421 aggctcgatc ttgcgtcttc gaggccatga tggaacatct ccattctcgt gcacatcaac
     6481 ggcccgaccg gcgtaggcaa gtcggcaatt gcagaaggcc tggtggagaa gcgaccgctc
     6541 gcactttgcc tcgacatcga cgcgctgcga gtccgcttgg ggcagtggcg cgagctcaac
     6601 gactccaagc agatcgcccg caacctcggc ttcgccctag tacagagaca tctcgaagct
     6661 ggacacgacg tcgtgctccc acagctcatc gttcgggaag atgttgtcga cgaccttgag
     6721 aaacttgcca tcgaagccgg tgcatccttc gtggaggtca ttctgcgcgc tgaaccacgc
     6781 gagctcgcgg cccgcgtcga acggtcccga gcggggcagg tcggcgagca cccacgagat
     6841 cagttcacgt ccgccgaact cgcacaacag acggagtacg cgcttgaggt gctggcgcag
     6901 atggcggctc gccgcatgaa cgtcactgtc gtcgacgtga gcggcatgtc cctcgcgcag
     6961 gcgactgcag ctgtggtggc agtgctggtc tgactgcccc acatggccgg ctcgaggaga
     7021 gatctcgccg cgccctacgc gtcccgcggg gaagtgcgtg tgccctgatg gaaagggagg
     7081 cgccagtctg cgtcgcggcg gatccacacg aagatccaca acgccgctgc tgtgtggtgc
     7141 tcgatcacgt acgtcaagag gatgacgtcg tctgccagct gcgtcgcctc acgattgcgt
     7201 cagcgcccct ggcgatgtcg gacgcgccga actcgacgaa gtccgagtgg aggagtccga
     7261 gcacgaggtc cggagccgtc cgcctcgggt gtgacccgtg ttgtccccca gcggcgcgtt
     7321 cgaggcgaac cgtcgatccg tcgcgcttcg gtgctgctct tgagaaaccg agctactaca
     7381 cgtagcgggc ggtagccatc gccaacgtcg cgaggatgat caaccacgcg ttgacaaggc
     7441 tggcggtgcg caagcgatgc ccgagtcggg ccagctcgtc ctgggcggtg gggtcggtgg
     7501 gcgcagcggc tgcgagcgcg gcggtccggg cgatgcccgg gcgaaggatg aggaaaccta
     7561 cgagccacgc agagagtgcg gcgaccgccc cggcggtgaa cattgcgccg gtccgagtag
     7621 agatccacgc ccactccagg cccgaggagt cgcgccagta gagaagcatc cctgcgacga
     7681 tggccagaac cgatacgacg gcgatggcga cagggaactt gcgcctctcc gtgagctcga
     7741 tcatgaactg actcgcggac ggtccgagcc ggttcgccac tggctcgatg aagaacgcga
     7801 acgaaagcga cgcaccgacc caggacgcag cggccacgat gtgcaccgct cgaagcccaa
     7861 ccacgtaggc gtccatcgtc cccctcgacc cagacctacc gaccggtcct cacctcgcgc
     7921 agcgtgcggg acgccgggcc ggcgcgcaat cccaggtttc tgggagacgg gcaggccgag
     7981 cagcgcagcg atagcgttcg gcgatgaggc cgccggacct gcaccgtctc cgtgctggcc
     8041 tctcggcggt cgtgggccgt cagctcggct tcatcgaatt cgccgcggag gcgacagagc
     8101 tgctcgctgc ggctgtcccg ttcgaccgca gctgctggca cacggttgat ccaggaacgg
     8161 tgttgttcac cgggagcgtg aaccagggga tcagttgctc cggccgctgg ctcgcggacc
     8221 acgagtacgt gatcgacgac gtcaaccagt ggtcgacgct cgcccgaaca ggtcaactcg
     8281 caggcaggtc gagcgctgct acctcgggcg acctcaaccg cagtgcccgg caccgctcca
     8341 cggcttcgtg gggcatcggc gacgagctcc gcgtgtcctt cgttgtcgat ggcgtctact
     8401 ggggtgccgc cggctttctc cgggacgacg atcggccgtg gttcaccgag gatgaggaac
     8461 aagagatgcg agccgtgatc gacctcctcg cgtcgggatg tcggcgggca ctcgtcggaa
     8521 ccggacacct cgtcagggac gccccgaacg tcaaagggcc cgccgtgatg atcctcgccg
     8581 acaacggcga agcggaggtg gtcacacccg cagctgcgct gtgggtgaac gacctgctgg
     8641 aggaacctcg tccgaggacc cccgcgcagt cgaaggtcgt acaggccgtg gcggctcagt
     8701 cgcgtgccag gggagatggc agcgatccga tcgccgcgcc ttcgcgagca agggtgcgga
     8761 cgaggtccgg cgagtggctc gtcgtgtacg ccacaacgct gtccggcgag accggagcgc
     8821 gcacggccat cgtgatccaa cctgcagtgc cggccgaggt tgcaccgctc ctcgcggtgg
     8881 cctacgggct cacgaaccga gagaaggatg tctcccggct ctgcttacag gggcggtcgt
     8941 ctaaggagat cgcgctcgcg ctcgggatca ccgtctacac cgtccaagat cacctgaagt
     9001 cgatcttcga gaagacggcc acgcggaccc ggaacgagct cgtcggccgc gtgttcttgt
     9061 cgcactacac gactcgctgg gaagagcacg ccgatgtacc tcccggatgg acggcgaaca
     9121 ttgccgctga agcggccacg ccccggacgc catcggcgga cgacgccaac tccggtggcc
     9181 gccgctgagc gacttcacgg aaagctcacc cgtagcgtgc gtgttcggtg atctgtgcgc
     9241 agccaccgcg gacctgtccc agaacccgcg tcgctgcgac cgtccagggt gccccacaag
     9301 gccgggagcg ggtgccgctc gcgaaagaac gttcggtccg ctcgtgcgtc gccaggctct
     9361 cttggtctcg tagtagtcga cgtcacccgc tggagagccg gcatatgcgc ccctatgcac
     9421 gagcggctcc cggacggtcc agacaagatg tgaggatggg ccgattcggc gcgattcgtg
     9481 agtcggttgt cacggtcgag gacgcgacga cctgcttcac ggagtggggc gatccagatg
     9541 gagcggtcgt agtcggctgg cccggcaacc caggctcgcg gttcggtctc ggctgggcac
     9601 gcctgtacgc atccgagtgt ggtgttcgcc tcttcgtctt cgaccgtccg ggcttcgggc
     9661 ggtcatcccc tgatccctcg cggaccgtgt cgtcaacggc aaggagaatc tgcgaggcag
     9721 tcgcgagcct cgggatcgag tcgttcggcg ttctcgggaa ctcctccgga ggcccgtacg
     9781 ctcttgcctg tggggccgtc gctccaggct ctgtaacttc catcgccgtc attgcggggg
     9841 gtggtcggat ggatgaggca ggctcgcaca gcggcatgtc gatcgagaac gacagattct
     9901 ggtccctcgc gagtcaagga ccctcgacga ctacgcccga cttcgaacag cttctccgaa
     9961 acgcaggggc gaatgcgaag ggcctccaca aggagcgcgt cgctgacatc gccgaagcgg
    10021 gcaggcaagg cgcggagcac ctcgcccttg atgcccacgt cattactgct cgatgggact
    10081 tcgacgagag tgacatcacc atgccggttg acctctggca cggcgcagcg gacgacgaca
    10141 ttccactgga acaggcgtgg cggctcgcgg accgtctgcc acgggcgacg ctgcacgtct
    10201 ggccgcgcca cggccacgac atgccacccg aggccatgac agccgtatac gcactgctgc
    10261 gctgaatcgt cttcggggcc gctccgaagt gccggtatct cttcgggcgg ctgcgtccgc
    10321 acccgcctga agagcgtcca cgatcccgaa gtactaggtg ccccacacgg ccggtacggc
    10381 gactcacggc atatcgacaa cccagggttg acggtaggca tgcgacaacc tagagttgac
    10441 gcatggcaac gaccctgtcg aacctcatcg atgaagttga gcaacgctcg ccatcgaaca
    10501 atccgctgga cctccttgcg acggcggcgg caaccgtcga ggaggtcact gacgccgcgg
    10561 atgcgttgct ctcccacttc gtagatcgaa ctcgccgggc cggacactca tggacggaaa
    10621 tcggtgccgc gctgggtgtc acaaaacaag cagtgcagaa gcgcttcacg ggcgatgaga
    10681 agcgcaaccc tcgcggctgg gagcgtttca ccgatcgggc tcgggctgtc gcctcgaaac
    10741 acgcgttcgc ggcgtccgaa gaactcggca acaactacgt gggcaccgaa cacctgcttc
    10801 tggcgatgtg gggcgagcca gaaggactcg cagcgctagc gctcgtcgac gctggactta
    10861 caagagaaac cgtgatcgag gcgatcgacg cccgcatccc tcgaggggcc cagggccgag
    10921 gcgggttcac gccgagggcg tgggtggcga tcgagaactc aacgaaggtc gcaatcgaat
    10981 tgggccacaa ctacgtcggc acagagcacc tcctcctctc gctcatgaca ggcgtcggcg
    11041 gagtggctga cgagatcctc ggcgagaagg gcgtgaaacg agacaaggtg gaagcgttcg
    11101 ttctcgccgc cctaaagagc tagaccccgt cggcctgaag gctacgccgc cacgctggca
    11161 agcactcctc ggacatagga gtcctgcccc ctatggccgg ctagcggacg ctggctactt
    11221 gcgagtgcgc atcgggaagt ggttaatcct cgatgcaccg attcgcttcg ggcaccggcg
    11281 gttgagaggc gttgccgtag ctcgcggtgc cgggtgtggt gttgatggcc tcgctggcgg
    11341 ggtcggggcc gcgtgtgtcc aagcggtgtg tcgacgactg gttgtc
//
LOCUS       DATLCO010000335         7496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10112590, whole genome shotgun sequence.
ACCESSION   DATLCO010000335 DATLCO010000000
VERSION     DATLCO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10112590"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VMZ33_07770"
     CDS             <1..405
                     /locus_tag="VMZ33_07770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUR17169.1"
                     /translation="SFYHARMPLLTDRGFAEDVAAAERVIKRACGVDPRPWFRCPFGA
                     GADDARTAQLLAEGGYRHVGWNVTGFDWLASRSGARLARDTVARVQDAGNGAIVLLHT
                     WPRSTALGLPQIIDTLGANGARFVGVDELLDA"
     gene            402..1394
                     /locus_tag="VMZ33_07775"
     CDS             402..1394
                     /locus_tag="VMZ33_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HUR17170.1"
                     /translation="MTVAVLAVDGGNSKTDVTLVERDGTVLSCVRGPTISHQVIGANA
                     GAVRLRELVDKAHRAAGREPHPAEMGVYCLAGADFHSDTRLLRRAFDKHALATTYEVL
                     NDCFAALRAGAPKGWGVVLICGQGINAAAVGPDGRSARFAGIGDLSGDWGGGGGLGAG
                     ALAAAIRARDGRAPRTALETAVASHFGKRTPEQVMLAMYEGRLDSARRRELAPAVFAA
                     AEDGDEVARALLDRLADELAGMAVALARRLRMTRLETDIVLAGGVFTATEPDFYARLY
                     ERTRAQIPDARFVRPTLPPVAGSALIGLDRILIDDADRHAAATRLALTFAQTEH"
     gene            1397..2344
                     /locus_tag="VMZ33_07780"
     CDS             1397..2344
                     /locus_tag="VMZ33_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR17171.1"
                     /translation="MTLNLGVLLWNMTPDWPTFERAAKRADELGYDHIWTWDHLHAIF
                     GEPQQPVFDGWMSLGALGKVTRRARLGLMVGANTFRNPGLVAKMATTLDHISDGRAIL
                     GLGGAWFTYEHTAHGIDFGKSPGERLRWLDEAASAMRTVLDGGSATSEEGSHYAFKDL
                     RQEPLPVQRRLPLMIGGSGERKTLRTVAKYADMWNMSGSVEKVAHKMAVLDQHCADVG
                     RDPAEIERTISCKLFLRSSEGESRQLLERTMEQNLTPLTNIESDDTFWVETPAQTAER
                     LIAYKRIGVTTFIPEIPAPYDDETLERLMTEVKPLVEAA"
     gene            2494..3069
                     /locus_tag="VMZ33_07785"
     CDS             2494..3069
                     /locus_tag="VMZ33_07785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HUR17172.1"
                     /translation="MADSPSVGTLSSIDSREYREVLTRFATGVTVVTTLEETDEGPQP
                     WGTTVNSFTGISLDPPLVMVSIGQERSIHPIIERIGKFGVNILGEDSQALSDCFAGAP
                     SSLPREAFCEADYSLGQTGLPVLESAIAYLCCELDRVIQAGDHTMYLGRVVETGCRNE
                     TGWPLLYFGRRYLSIERATAPDLRGKPDSLR"
     gene            3078..3977
                     /locus_tag="VMZ33_07790"
     CDS             3078..3977
                     /locus_tag="VMZ33_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR17173.1"
                     /translation="MPYVRANGLDIHYEVEGAGPPMVLLHGATSSASEDWAAQRPLFR
                     KAFRIHLVDARGHAGTRWDVADGFSRDMLVDDLLAFVDALHLQTFHLIGFSMGAMTSL
                     AFATRYPERLRTAVISGIDVQREPRARVAKRLMDPERILREEPGWASQLERRHAPVQG
                     SGAWQRLLPAIADDVATAALLTPEDLRNVRLPVLLVYGDRDIFVPVDHAYDIQRQLPD
                     ARLLISPDTGHQVMATQPALFNLAAGNFYRSTEKVARERAEAGADLFADSSESAGDGA
                     TVYEPGAVTVTTIEDPAEPFSTW"
     gene            4001..4318
                     /locus_tag="VMZ33_07795"
     CDS             4001..4318
                     /locus_tag="VMZ33_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HUR17174.1"
                     /translation="MPATLAAMYKRPEGGDEALTNFWQRYHDEHLPLIAKLPGLRGTK
                     VWNVTRKYAGEDLVAVCFMEFDDNDALKAAMKSDEMGAAGANLAEIAPDLLTLVALEE
                     SWQ"
     gene            4315..5121
                     /locus_tag="VMZ33_07800"
     CDS             4315..5121
                     /locus_tag="VMZ33_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HUR17175.1"
                     /translation="MTVHYEIAGRVATVRIDRPEVFNALDRPTLEAIRDSFRQASADH
                     HVGVVVLTGTGDRAFSTGADLDEQEQFLARPNDYWDWMGRFIEAIEAIKDCAKPVVAR
                     LNGMVVGGGNELNIACDLAIAVDEAVFRHVGPSRGSLPAGGATQWMPLLVGDRRAREV
                     LLLNRPVSAQTALDWGWVNRVVPRAELDAAVADYCDELLAKMPEIIRATRVQLNYWKT
                     QSWAATVPMAREWLTVHAGSAEVAEGLASFHEKRHVDYERLRGDVEEAGS"
     gene            5118..5921
                     /locus_tag="VMZ33_07805"
     CDS             5118..5921
                     /locus_tag="VMZ33_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUR17176.1"
                     /translation="MTPEKPADKPRIRVERPPDHDGVALVWLDRPEVLNALDYRTLGE
                     LVEALEMLDSDESVRCVVVTGAGDRAFAAGADIKEMADATPVSMSVTNNFGRWEKIRR
                     VRVPIIAAVRGFALGGGCELAMACDMVVAADDATFGQPEIKIGIMPGAGGTQRLTRAL
                     GKAKAMEMILTGRNLSAQAAYERGLVSAVVAREETVSEALKLASEVASMPPLAVRAAK
                     ESVNRAHELSLDAGLEFERRNFFLLFASDDQKEGMAAFVEKRKPTWKGR"
     gene            6140..6421
                     /locus_tag="VMZ33_07810"
     CDS             6140..6421
                     /locus_tag="VMZ33_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HUR17177.1"
                     /translation="MTVNVTERDYAIELDTVTMTAPVTFAITNSGPGPHAFAISDASG
                     AVLATTHEISRAKTGQLIVPVLEPGEYTYYCPIVGHKREGMVGTLTITP"
     gene            complement(6435..6770)
                     /locus_tag="VMZ33_07815"
     CDS             complement(6435..6770)
                     /locus_tag="VMZ33_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17178.1"
                     /translation="MLEAAEGAAELPAEGAVEPPSAEGAVDEPAEGAAEVPVDGRVVL
                     LAPPPAHAPTINATATRDKPNLDGFIEFSCEVVYRVRPRVGAVIGVFAGEPRMVQIER
                     LTNPLRAQT"
     gene            6814..>7496
                     /locus_tag="VMZ33_07820"
     CDS             6814..>7496
                     /locus_tag="VMZ33_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17179.1"
                     /translation="MGPESPAASDDGSASSDITSAAATLRTDLNYLLGEHQIIAFKAT
                     GAALGGRQEQFEAYGALLNTNGTDLGAAIGSIYGTEAEDEFNRIWSAHNGFFVDYTTG
                     VATDDMAMQDQAVSDLTTIYVPEFTTFLVGATGLPEDAVTQLVTDHVIHTKEGVDAQA
                     SGDFEAAYAAIREAYAHISEIGNALAPAIAEGNDIEGDATTPAAGFRTALNNMLQEHL
                     FLASFATDAA"
BASE COUNT         1276 a         2511 c         2457 g         1252 t
ORIGIN      
        1 tccttctatc acgctcgcat gccgctcctg acggaccgtg gcttcgccga ggacgtcgca
       61 gccgcggagc gcgtcatcaa gcgtgcgtgc ggggtcgatc cgcgtccctg gttccgctgc
      121 ccattcggtg ccggtgctga cgatgcccgc accgcgcagc tcctcgccga gggcggctac
      181 cgacacgtcg gctggaacgt caccggtttc gattggctcg ccagccgctc aggagcacgc
      241 ctcgcgcgcg acaccgtcgc ccgcgtgcag gacgctggca acggggccat cgtcttgctg
      301 cacacgtggc cgcgttccac ggcattgggg ctgccgcaga tcatcgacac gctcggcgca
      361 aacggcgcac gctttgtcgg agtggacgag ctactcgacg catgaccgtt gccgtgctcg
      421 ccgttgacgg cggcaactcg aagacggacg tgaccctcgt ggagcgcgac ggcacggttc
      481 tgtcttgcgt tcgtggcccg accatctcgc accaggtcat cggtgccaac gccggcgcag
      541 tccgcttgcg ggagcttgtc gacaaagcgc accgtgccgc gggccgcgag ccccatccgg
      601 ctgagatggg cgtctattgc ctcgccgggg cagactttca ttctgatacg cgactgctcc
      661 ggcgtgcctt cgacaaacat gcgctggcga ccacgtacga ggtgctgaac gactgcttcg
      721 ccgctttgcg cgcgggcgcg cccaaaggct ggggtgtggt gctcatctgc ggccagggaa
      781 tcaacgccgc ggcggtcggc ccggatggac gctcagctcg gttcgcgggc atcggcgacc
      841 tgtccggtga ttggggcggc ggcggcgggc tcggcgctgg ggcgctggct gccgcgatcc
      901 gtgcccgcga cgggcgcgcg cctcgaacgg cgctggagac cgccgtggcg agtcatttcg
      961 gcaagcggac cccggagcag gtgatgctcg caatgtatga gggccggctc gacagcgcgc
     1021 gccgtcgaga gcttgccccg gcggtctttg cggcggctga ggacggcgac gaggtcgcac
     1081 gcgccttgct tgatcgcttg gccgatgagc tcgccgggat ggccgtggcg ctggcgcgcc
     1141 gactgcggat gacccgactg gagacggaca tcgtgcttgc cggcggagtg ttcaccgcca
     1201 ccgagcccga tttctacgcg cgactgtatg agcgcacgcg tgcacagata cccgacgcgc
     1261 ggttcgtccg gcctacgctt ccgccggtcg cgggctcggc gctgatcggc cttgatcgca
     1321 tattgataga cgatgccgac cgccatgccg cggccactcg cctggctttg accttcgcac
     1381 aaacggagca ctagtgatga ccctcaacct cggcgttctc ctgtggaaca tgacgcctga
     1441 ttggccgacc ttcgagcgcg ccgcgaagcg agccgacgag ctcgggtacg accacatctg
     1501 gacgtgggac cacctgcacg cgatcttcgg cgaaccccaa cagccggtgt tcgatggctg
     1561 gatgtcgctc ggcgccctgg gcaaggtcac gaggcgtgcg cgactggggc tgatggtcgg
     1621 tgccaacacg ttccgcaacc ccgggctggt tgccaagatg gcgacgaccc tcgatcacat
     1681 cagcgatggc cgggcgatcc tcggcctggg cggggcgtgg ttcacgtatg agcacaccgc
     1741 gcacggcatc gacttcggga aaagcccggg cgagcggctg cgctggttgg acgaggccgc
     1801 gtctgcgatg cggaccgtgc ttgacggtgg ctcggccacg tcagaggagg gcagccacta
     1861 cgcgttcaag gacctccgcc aggaaccgct cccagtccag cggcggctgc cgttgatgat
     1921 cggcggatcg ggcgagcgca aaacgctgcg aaccgtggcc aagtacgcag acatgtggaa
     1981 catgtctggt tcggttgaga aagtggcgca caagatggcc gtgctcgacc agcactgcgc
     2041 ggacgtcggc cgtgatcctg ccgagatcga gcgcacgatt tcgtgcaagc tgttcctgcg
     2101 gagctcggag ggggagtccc gtcagctcct cgagcgaacg atggagcaga acctgactcc
     2161 actgaccaat atcgagagcg acgacacctt ctgggtagag acccccgcgc agacggcgga
     2221 gcggctgatc gcctacaagc ggatcggtgt caccacgttc atccccgaga tcccggcgcc
     2281 gtacgacgac gagacgctcg agcgactcat gacagaggtg aagcctctcg tcgaggcggc
     2341 gtgaggcaga cgctcacagc gtcttgatga agcgcttcga actcaccacg tagcccgaat
     2401 cgacgagccg agctggtcaa gcagatctgc aatccgtggg gcatcggagc ccgtggcggg
     2461 tcggaccctc atgcgggcta gactcgcagt tctatggcgg attcgcccag cgtaggcacg
     2521 ctctccagca tcgactcacg ggaataccgc gaagtcctga cgcggttcgc cacgggcgtc
     2581 accgtggtca cgacgctcga ggaaacggac gaaggcccgc agccctgggg cacgaccgtc
     2641 aactcattca cgggcatctc gctcgatccg ccgctggtga tggtctcaat cggccaggag
     2701 cgctccatcc acccgattat cgagcggatc ggcaagttcg gcgtgaacat cctcggcgag
     2761 gattcacagg ccctgtcgga ctgcttcgcc ggtgccccat ccagcctgcc gcgcgaggcc
     2821 ttctgtgaag ccgactactc gcttggacaa accggcctgc ctgtgctcga gtcggccatc
     2881 gcgtacctct gctgtgagct cgaccgggtc atccaggcgg gcgaccacac gatgtacctc
     2941 ggccgcgtag tcgaaaccgg ctgccggaac gagaccggct ggccgttgct gtacttcggt
     3001 cgtcgctatc tcagcatcga gcgcgccact gcgcccgacc tgcgcggcaa gcccgactcg
     3061 ctgcgctaga gcgccaagtg ccctacgtcc gcgccaatgg gctcgacatc cactatgagg
     3121 tggagggcgc cggaccgccc atggtgctgc tgcacggggc gacctcttcg gcgtcggagg
     3181 attgggcggc gcaacggccg ttgttccgca aggcgttccg catccatctc gtcgatgcgc
     3241 gcggccacgc cgggacgcgc tgggacgtgg ccgacggctt ttcgcgcgac atgctggtcg
     3301 atgacctgct cgctttcgtg gacgccctcc acttgcagac cttccacctg atcggcttca
     3361 gcatgggtgc gatgacctcg ctggcgttcg cgactcgcta cccggaaagg ttgcgcaccg
     3421 ccgtgatcag cggcatcgac gtccagcgcg agccgcgcgc gcgtgtcgcg aagcgactca
     3481 tggatcccga gcgaatcctg cgcgaggagc ccggttgggc ctcacagctg gaacggcgcc
     3541 acgcccccgt tcagggctcc ggcgcctggc aacgcctgct gccggcaatc gcggatgatg
     3601 tggccacagc tgcgctgctc acacctgagg atctgcggaa cgtccggctg ccggtcctgc
     3661 tggtctacgg cgatcgcgac attttcgtgc cggtcgatca tgcgtacgac atacagcggc
     3721 agctgcccga tgcgcggttg ctcatctcgc ccgataccgg ccatcaggtg atggctacgc
     3781 aaccggcgct cttcaacctg gccgcgggta acttctatcg gtcgactgag aaagtggcac
     3841 gcgagcgcgc cgaagcgggc gccgatctgt tcgcagattc cagcgaatcc gctggcgacg
     3901 gcgccaccgt ctacgaaccg ggagcggtaa cagtcacgac tattgaggac cccgccgagc
     3961 ccttcagcac ctggtagtca cgagcacgga ggaggagcaa atgcccgcca cactggcagc
     4021 gatgtacaag cgtcccgagg gcggcgacga ggccctcaca aatttctggc agcgctacca
     4081 cgatgagcac ctgccgctca tcgcgaagct gcccggcttg cgcggcacga aggtctggaa
     4141 cgtcacgcgg aaatacgcgg gcgaggatct ggtggccgtg tgcttcatgg agttcgacga
     4201 caacgacgcg ctcaaggccg cgatgaagag cgacgagatg ggcgccgctg gtgccaatct
     4261 ggccgagatc gcgcccgacc tcctgaccct cgtcgcgctt gaggaaagtt ggcagtgacc
     4321 gttcattacg aaattgctgg ccgcgtcgcg accgttcgca tcgatcggcc ggaggttttc
     4381 aacgcgcttg atcggccgac tctcgaggcc attcgcgact cgttccgaca ggcgtccgcc
     4441 gaccatcatg tcggcgtcgt cgtcctcacc ggaaccggcg accgcgcctt ctccaccggt
     4501 gccgatctcg acgagcagga acagttcctt gcccgcccca acgactactg ggactggatg
     4561 gggcgcttca tcgaggccat cgaggcgatc aaggactgtg ccaagcccgt cgtcgcgagg
     4621 ctgaacggca tggtcgtcgg tggcggtaac gagctgaaca tcgcgtgcga cctggccatc
     4681 gccgtggacg aagcagtgtt ccgtcacgtc ggcccgtcgc gcggctccct gccggccgga
     4741 ggcgcaacgc agtggatgcc gctgctcgtt ggggaccgcc gggcgcgtga ggtgctgctg
     4801 ctgaatcggc cggtctcagc ccagactgct ctcgactggg gctgggtcaa ccgagttgtg
     4861 ccgcgtgccg agcttgatgc cgccgtcgcc gattactgcg atgagctcct ggccaagatg
     4921 cccgagatca ttcgcgccac gcgagtgcag ctgaactact ggaagacgca gtcgtgggcg
     4981 gcgacggtgc ccatggcgcg cgagtggctg actgttcacg ccggctctgc ggaagttgcc
     5041 gagggccttg cctcgttcca cgagaagcgc cacgtcgact acgagcggct gcgcggtgac
     5101 gttgaggagg caggctcatg acgcccgaga agccggccga caaaccgcgc atacgggtcg
     5161 aacgaccacc cgaccacgac ggcgtcgcgc tggtctggct cgaccggccg gaggtgctga
     5221 acgcgctcga ctatcggact ctgggcgagc tcgtcgaggc gctggagatg ctcgattcgg
     5281 atgagtcggt ccgctgcgta gtcgtcaccg gcgccggcga ccgcgccttc gcagcgggcg
     5341 ccgacatcaa ggagatggcc gacgccactc cggtcagcat gtcagtcacg aacaacttcg
     5401 ggcgctggga aaagatccgg cgcgtccgcg tgccgatcat cgcggcggtc cgcggctttg
     5461 ccttgggcgg cggctgcgag ctggcgatgg cgtgcgacat ggtcgtcgcg gctgacgacg
     5521 cgacgttcgg ccagcccgaa atcaagatcg gcatcatgcc cggtgccgga ggcacgcaac
     5581 gtttgacgcg ggcactgggc aaggccaagg cgatggagat gatcctgacc ggccgcaacc
     5641 tctcggcgca ggcggcatat gagcgcggcc tcgtctcagc ggtcgtcgcc cgcgaggaga
     5701 ccgtctcaga agcgctgaag ctggcatcag aggtggcgtc catgccgccg ttggccgttc
     5761 gcgcggcaaa ggagtccgtc aatcgggcgc acgaactttc gctcgatgcc ggtctcgagt
     5821 tcgaacgccg taacttcttt ctcctgttcg cctcggacga ccagaaagag ggcatggcag
     5881 cgttcgtgga gaagcgcaaa cccacgtgga agggccgcta gggctagccc agcgacttga
     5941 cccgtcgtaa agtccgcgca tcgctgctcg gctgtcatcc gagagccaaa atgaggatgg
     6001 agcttaagtg aagcggtcag tccggtctgt cctgttcggc ctagcggccc tcgctctcgc
     6061 aggctgtggc gggggaggcg gagcgccgcc agttatcacc tctgcgccgg ccggtgcttc
     6121 gcctggggca ccaatcggca tgaccgtcaa cgttacggag cgtgactacg cgattgagct
     6181 cgacacggtc accatgacgg cacccgtcac attcgccatc acgaattcgg gccctgggcc
     6241 gcacgccttc gcgatcagcg atgcgtcggg agcggtgctt gcgacgactc acgaaatctc
     6301 acgcgccaag accgggcagc tcatcgtgcc cgtcctcgaa ccgggtgagt acacgtacta
     6361 ctgccccatc gttggacaca agcgcgaagg gatggtgggg acgctcacga tcacgccgta
     6421 gggtcgacgt ctgatcaggt ctgcgcccgt aacggatttg taagccgctc aatctgaacc
     6481 atccggggct ctcccgcgaa cactcctatg accgcaccaa ctcgcggtcg tactcgataa
     6541 accacttcgc aggagaactc aatgaaacca tccaggttcg gcttgtccct tgtcgctgta
     6601 gcgttgatcg tcggcgcgtg cgccggcggc ggcgccagca acaccacccg cccgtcaacg
     6661 ggcacctcgg cagccccttc ggctggctcg tcaaccgccc cctcagcgga cggcggttca
     6721 accgctcctt cggcaggcag ctccgctgcg ccctcggcgg cctcgagcat ggcccctgag
     6781 acatcaggcc cggccagtga ggcccctcat tcgatgggcc ccgagtcgcc cgcagcgtct
     6841 gacgatggca gcgcttcgtc cgacatcacc agcgccgccg cgactctgcg caccgacctc
     6901 aactacctgc tcggcgagca ccagatcatc gccttcaagg caaccggcgc tgccctcggc
     6961 ggccgccagg agcagtttga agcctacggc gctctgctga acacgaacgg caccgatctt
     7021 ggcgccgcca tcggcagcat ctacggcaca gaggccgagg atgagttcaa ccgcatctgg
     7081 agcgcccaca acggcttctt cgtcgactac acgacgggtg tcgcgaccga cgacatggcc
     7141 atgcaggatc aggccgtttc cgacctgacg accatctacg ttcccgagtt caccacattc
     7201 ctcgtcggcg ccactggcct gcctgaggat gccgtcaccc agctggtcac cgaccacgtg
     7261 atccacacca aggaaggcgt cgatgcgcag gcgtcaggcg acttcgaagc tgcttatgca
     7321 gccatccgcg aggcctacgc gcacatctcc gagatcggca acgcgctggc cccggcgatc
     7381 gctgaaggca acgacatcga gggcgatgcc acgacgccgg cagccggctt ccgcactgcc
     7441 ctcaacaaca tgctgcagga gcacctgttc ctcgccagct tcgcgactga cgcggc
//
LOCUS       DATLCO010000336         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6382563, whole genome shotgun sequence.
ACCESSION   DATLCO010000336 DATLCO010000000
VERSION     DATLCO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6382563"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..857
                     /locus_tag="VMZ33_07825"
     CDS             141..857
                     /locus_tag="VMZ33_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17180.1"
                     /translation="MTDSSTTAQVLHDIPALRRGWRPQTVKRGRFVPQIADPILEPLW
                     SGTRVLAHFRDAQNEDEWGTVIAIDEDGEDASPDAPMALDHLRRAVQAREAVIDGVIT
                     GEATTSGENTAIKLFPTVNPIRKFFLGGTPDSDVKYEPRGPRPRGVPAFVAVDLLSID
                     EQPLFDVPLLERKRILEGLIEESDLVRLSPWVRPPVRTWFNTWRAAGLRGLIMKSSNS
                     RYRPGEETTEWAYVERMPRS"
     gene            824..1972
                     /locus_tag="VMZ33_07830"
     CDS             824..1972
                     /locus_tag="VMZ33_07830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR17181.1"
                     /translation="MGVRGADAAILTPTPGKDRSWPLVVRRAREDDRDAVLSFATRTW
                     HDWDYIPHAWPIWLEAKDGAFLVGTVGEPADVSPASLQDAEGNPLAVGQVVAITRVAM
                     VSRTEAWLEGIRVDPRVRGMGVASDLQIAELHWVAGQDAQMLRYATGSNNEGSHRLGA
                     RDGIVVVARFHAWWWSATGNAEDDADELSAFDSAVREQTTRNRQAALQSLAEVGLVVG
                     AAGFDRLWQMIDNDPTFIAGQRLYEPRPWGLQELTPELFHRHMMRGEVIADGHEAVAI
                     LVGEQLPAEDSSLRLATLVGRGPAAAELAGRMRRVIGYPFRFRVPVESPMIAGHEQLF
                     RNEGFVTPEWELHVLARPMEDPEQPIPADDPRRVVLAEPPDALEPPRW"
     gene            1983..>2410
                     /locus_tag="VMZ33_07835"
     CDS             1983..>2410
                     /locus_tag="VMZ33_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17182.1"
                     /translation="MPSTALPQALREEVARLGSADIMVGIPSFKNAATIGYVTRAAQA
                     GLVQYFPDLQPVLVNADAGSTDGTQRVVVETEPPDYIEQILLVRPRNKLRRISLTYPE
                     VDGVGGKGAALRTIFQMANALKVQALVVVDSDLRSIIPEW"
BASE COUNT          413 a          805 c          809 g          383 t
ORIGIN      
        1 aacgggcggg agagatgttg ggttggcagc cgcggcgagg tacgctcacc gagatgatcg
       61 gaagcgcttg gcgctggcac gagggacacc cgaacggcta cgggagctga cagccatgag
      121 gcccgagatg agtgaccgca atgactgaca gcagcacgac ggcgcaagtg ctgcatgaca
      181 ttccagcact acgccgcggc tggcgaccac agaccgtcaa gcgcggccgt ttcgtccccc
      241 agattgcgga cccgatcctg gagccgctct ggtcagggac gcgggtcctg gcccattttc
      301 gcgacgccca gaatgaggac gagtggggca cggtcatcgc gattgacgaa gacggcgagg
      361 acgcctcacc ggacgctccg atggctctcg atcatctgcg tcgtgccgtg caggcgcggg
      421 aggcggtgat cgacggcgtc atcaccggtg aggccacgac cagtggcgag aacaccgcga
      481 tcaagctttt cccgacggtc aacccgatcc gcaagttttt cctcggcggc acgcccgact
      541 cagacgtcaa gtacgaaccg cgcggaccca ggccacgtgg cgtgccggcg ttcgtggccg
      601 tggatctcct gagcattgac gagcagccac tgttcgacgt gccgctcctc gagcggaagc
      661 gaatccttga agggctcatc gaagagagcg acctcgttcg gctctcgccg tgggtccggc
      721 cgccggttcg cacgtggttc aacacgtggc gtgccgcggg cctccgcggc ctgatcatga
      781 agagctccaa cagtcgttat cgaccgggtg aggaaacgac cgaatgggcg tacgtggagc
      841 ggatgccgcg atcctgaccc cgacgcctgg aaaagaccgt tcctggccgc tcgttgttcg
      901 tcgagcgcgc gaggacgatc gcgacgcagt cctgagcttt gccactcgaa cgtggcacga
      961 ctgggattac atcccgcacg cctggccgat ttggctcgag gcaaaggacg gcgccttcct
     1021 ggtcgggact gtcggcgaac cggccgacgt ctcccccgcc agcctgcagg acgcggaggg
     1081 caacccgctg gccgtcgggc aagtcgtcgc gatcacccgg gtggccatgg tctcgcgcac
     1141 agaggcgtgg ctggaaggca tccgtgtcga ccccagggtt cgcggcatgg gcgtcgccag
     1201 tgacctgcag atcgccgagc tgcactgggt agcgggccag gacgcacaga tgctgcgcta
     1261 cgcgacggga tccaacaacg agggctcgca ccggctcggc gcgcgcgacg gcatcgtcgt
     1321 tgtcgcccgc ttccacgcct ggtggtggag cgctaccgga aatgccgagg acgacgccga
     1381 tgagctgagt gccttcgact cagccgttcg agagcagacg acccgcaatc gccaggccgc
     1441 gctccagtct ctagccgagg ttggtctcgt cgttggcgcc gccggtttcg accgtctctg
     1501 gcagatgatc gacaacgacc ccacattcat tgcggggcag cgtctgtacg agccgcggcc
     1561 ctgggggttg caggagctca cgcccgagct gttccaccgt cacatgatgc gcggcgaggt
     1621 catcgccgac ggccacgagg ccgtggcgat cctcgtcggc gagcagctcc cggccgagga
     1681 ttcatctctc cggctcgcaa ccctggtggg gagaggcccg gcagctgccg agctggccgg
     1741 acggatgcgt agggtgatcg gctacccgtt ccgcttccgc gtacctgtcg aatcgccgat
     1801 gatcgccggc cacgagcagc tgttccgcaa cgagggcttt gtgacccctg agtgggagct
     1861 tcacgtcctg gcgcgcccga tggaggatcc ggagcagcca atcccggccg atgacccacg
     1921 acgagtggtc ctcgccgagc caccggacgc cctcgaacca ccccgctggt aaggtcaggc
     1981 tgatgccgtc gaccgccctt ccgcaggcgc ttcgcgaaga ggtcgctcgc ctcgggagcg
     2041 ccgacatcat ggtcggcatc ccctcattca agaacgcagc gacgattggc tacgtcacgc
     2101 gcgcggcgca ggcgggcctc gtgcagtact tcccggacct gcagcccgtg ctcgtcaatg
     2161 ccgatgccgg atcgaccgac gggacgcaac gcgtcgtcgt tgagaccgag ccgcccgact
     2221 acatcgagca gatcctgctc gtccggccgc gcaacaagct gcgccgcatc tccctgacgt
     2281 acccggaggt cgacggcgtc ggcggcaagg gcgccgcgct gcgcacgatc ttccagatgg
     2341 cgaacgccct gaaggtgcag gcgctggtcg ttgtcgactc cgacctgcgc tcgatcatcc
     2401 ccgagtggat
//
LOCUS       DATLCO010000337         5291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10911616, whole genome shotgun sequence.
ACCESSION   DATLCO010000337 DATLCO010000000
VERSION     DATLCO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10911616"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VMZ33_07840"
     CDS             complement(<1..1161)
                     /locus_tag="VMZ33_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HUR17183.1"
                     /translation="MTDLVLGRKPPPRARLGFRFAVFGLIVVLVVGLLTTRLFYLQVV
                     EGGYYAGLATENGRALIPLRSTRGLIYDRAGRQLAVNIPSYVVRIRPADLPFSQRDGV
                     VSRLSSLLNVPAADLIETLDRQANNSFELVRLANDVPDDVARIITEESGSLPGVQVSV
                     EERRQYAYGPLVAHVMGYTGSVTADELEAFGDLGYLNDDQIGKTGVEATFEEVLRGTY
                     GEQEVEQDALGRILRTTEVTREPDPGNSIELTIDVEIQREAEEALKWATDIVNLQRGV
                     VIVMNPQTGEILALVSLPNYDNNLFASGISGADYQALLEDPNRPLVNFGLSEQYPPGS
                     TYKLVTGSGALEDGIIKDSTLVETAGYIEIGTYRYYDWNRRGFGPLDIYGGFA"
     gene            complement(1165..1665)
                     /gene="mreD"
                     /locus_tag="VMZ33_07845"
     CDS             complement(1165..1665)
                     /gene="mreD"
                     /locus_tag="VMZ33_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HUR17184.1"
                     /translation="MTLPFSALVAVIAALLETSVLAELPLAGATIDLVLVCAAVATLV
                     LGVEDGLAIAFLGGLLVDMVVPDRPMGAATLAILLALGLAFVVSRAMGPWRRLVVVGL
                     ILILTPVYHVLFTGIMALVANADLAIDISTVLIAAVLNAVLAIPISAVFGALERRFGA
                     AERAAW"
     gene            1935..2729
                     /locus_tag="VMZ33_07850"
     CDS             1935..2729
                     /locus_tag="VMZ33_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR17185.1"
                     /translation="MGEVIACANQKGGVGKTTTVINLAAYLALAGRRVLVIDLDPQGN
                     ATSGLGHDRSQPEGSIYAALLGREPTPNLIVDTQIDGLSLIPSSGDLAGAEIELIALA
                     QRERRLQQALNDVREKFDFVLLDCPPAVGLLTVNALTAADGVLVPIQTEYYALEGLSQ
                     LLSTINLVRDNLNPRLRLAGVLLTMYDARTTLSADVAAEVRRHLGATVFEVIVPRSVR
                     LAEAPSYGRPIARYSPESRGAQAYQALAEELLGRTAQEQKAIMGAL"
     gene            2726..3589
                     /locus_tag="VMZ33_07855"
     CDS             2726..3589
                     /locus_tag="VMZ33_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUR17186.1"
                     /translation="MTTDLGRNTSGLGRGLGALIPQRDDSAKPGVQELPISAIASNPY
                     QPRHAIEQSQLEDLTASIREHGVLQPVLVTQTPGGYRLIAGERRLRASDAAGLTHIPA
                     VVRSATESEQLAWALIENIQRSDLNAMEAAHAFRRLGDEFGLTHEEISQRVGRARSTV
                     SNTLRLLDLAPEVQSAVSSGAVSEGHARAIGGLSEHGLQAEIVGLVVKRGLSVRQTEE
                     LIRRARDGKPTRSQGAATPDVERLERGLREALSTKVTLNSSRKGGRITIEYYDEDDLE
                     RIYERLTGERP"
     gene            3586..>5291
                     /locus_tag="VMZ33_07860"
     CDS             3586..>5291
                     /locus_tag="VMZ33_07860"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HUR17187.1"
                     /translation="MTDSVASRPQAQSAGNGRRGKGAASDYTAESIQVLEGLEAVRKR
                     PGMYIGSTDARGLHQLVWEIVDNSIDEAMAGHANYIDLTIRADGSVHNRDNGRGVPVG
                     LQKQTGRDALEVVHTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSISLKVESARDGK
                     LWRQEYSRGKPTTKVTAAGTSAGRTGTTTEFMPDAEIFETLDFNFDTIAQRLRESAYL
                     NKGVWIRFVDQRVTPARERSFFFEGGLVSFVRHLNKGRDVLHPKPIYVEKRDGSTQIE
                     IALQYNDGYSESVFAFANNINTVDGGTHITGFRAALTSSLNDYARKNTILKDADANLS
                     GEDVREGLTAVISVKLTDPQFEGQTKAKLGNAEVKGQVQAALTEGIFQYLDENPQDAR
                     RIIEKSLIAARAREAARKARDLVIRKSALEGSALPGKLADCQERDPARSELFIVEGDS
                     AGGSAKQGRDRHFQAVLPMFGKMLNVEKARIDKVLSSEKIRPLIIALGAGIGETFDIA
                     KLRYHKIILLADADVDGAHIRTLLMTFFYRHMPEVVGSGHLYIAQPPLYRISTGKVTR
                     YA"
BASE COUNT         1042 a         1649 c         1735 g          865 t
ORIGIN      
        1 ggcgaagccg ccgtagatgt ccagcggtcc gaaaccacgt cggttccagt cgtaatacct
       61 gtacgtgccg atctcaatgt agccagcggt ctccacgagc gtggagtctt tgatgatccc
      121 gtcctcgagc gcgcccgaac cggtgacgag cttgtaggtc gatcccggtg ggtactgctc
      181 ggagaggccg aagttcacga gcgggcggtt cggatcctcg agcagcgcct ggtaatcggc
      241 gcccgagatg ccactggcga acaggttgtt gtcgtagttg ggcagtgaca cgagggcgag
      301 gatctcgccg gtttgcgggt tcatgacgat gacgacgccg cgctgcaggt tgacgatgtc
      361 ggtcgcccac ttcagcgcct cctctgcctc acgctggatt tcgacgtcga tggtgagctc
      421 gatggagttg cccggatcag gctcgcgcgt gacctcggtc gtgcgcagga ttcggccgag
      481 cgcgtcctgc tcgacctcct gctcgccgta cgtgccgcgc aggacctcct cgaacgtggc
      541 ctcgacaccg gtcttgccga tctggtcgtc gttgagatag ccgagatcgc cgaaggcctc
      601 caactcgtcg gcggtcaccg agccggtata gcccatgacg tgtgctacga gggggccgta
      661 ggcgtactgg cggcgctctt cgacgctgac ctggacaccg gggagggagc cactttcctc
      721 ggtgatgatc cgcgcgacgt cgtccggcac atcgttggca agccggacga gctcgaagct
      781 gttgttggcc tgacggtcga gggtttcgat taggtccgcc gcgggaacgt tgagcagcga
      841 ggagaggcgg ctgacgacac cgtcacgttg gctgaagggc agatcggcgg gccgaatccg
      901 aacaacgtac gagggaatgt tgacggccag ctggcgtccg gcgcggtcgt agatgaggcc
      961 gcgcgtcgag cgcaacggga tcagggcgcg gccattctcc gttgcgaggc cggcgtaata
     1021 gccaccctca acaacctgga gatagaagag gcgggtcgtc agtaggccaa cgaccaatac
     1081 gacgatcagg ccgaagacag cgaagcggaa gccgaggcgc gcgcgtggcg ggggctttcg
     1141 gcccaggacg aggtcagtca tggattacca agcggcacgc tcggccgcgc cgaagcgacg
     1201 ctccagagca ccgaatacgg ctgaaatcgg tatggcgagt acggcgttga gcacggcagc
     1261 aatgagaacc gtgctgatgt cgatcgctag gtcagcgttg gccacgagcg ccatgatccc
     1321 ggtgaagagc acgtgataga ccggtgtcag gatcaggatc aggcccacga caaccaggcg
     1381 gcgccaaggt cccatcgcac gtgatacgac gaatgcgagg cccagcgcga gcaggatcgc
     1441 gagcgtggcg gcgcccatcg gtcggtcggg gaccaccatg tcgacgagga gtccgccgag
     1501 aaacgcaatg gccagaccgt cttccacacc caacacaagg gtggcgacgg cagcgcagac
     1561 caaaacaagg tcgatggtcg cgcccgcgag cggcagctcg gcgagcacgc tcgtttccag
     1621 gagcgcggca atgaccgcta cgagcgcgga aaaaggcaga gtcaagaagg gtctccggtg
     1681 gcgtctcgga gtataggcgc ggtccctgtg agcccattca ggtggtgaaa tcgcacgaat
     1741 gttcgaaatt gttcgccgtg aaacagccac gtgacagttg gttggtgctt ggttgcttca
     1801 cgtgaaacat tccaatgggg taaggctcga ggaagtgccc gataaggcgc tgatgaggga
     1861 gcgtgccgac actccggaat cgggtgagtc cgatagaatc aatcctagtg aatgagcgtt
     1921 tctccggccg ttgattgggc gaggtcatcg cctgcgcgaa tcaaaagggt ggcgtcggca
     1981 agacgacaac cgttatcaat ctggcggcgt atctcgcact cgcagggcgg cgcgtcctgg
     2041 tcatcgacct tgatcctcag ggcaacgcca cgagcgggct cggacacgac cgttcgcagc
     2101 ccgaaggctc gatttatgca gccctccttg gccgtgaacc gactccgaat ctcatcgtcg
     2161 acacgcagat cgacgggcta tcgctcatcc cttcgagcgg cgacctcgcc ggtgcagaaa
     2221 ttgagctcat tgccctcgcc cagcgcgaac ggcgtcttca gcaggccctc aacgacgtgc
     2281 gggagaagtt cgatttcgtg ctcctcgact gccctccggc agtggggctc ctcacggtca
     2341 acgcgctcac agctgccgat ggtgtgctgg tacccatcca aaccgagtac tacgcattgg
     2401 aggggctcag tcagctgctt tcaaccatca acctcgtccg cgacaacctc aatccccggc
     2461 tacgcctggc aggcgtgctg ctgacgatgt acgacgctcg aacgacccta tcggccgacg
     2521 ttgcagcaga agttcgacgc caccttggcg cgacggtttt cgaggtgatc gtgccgcgca
     2581 gtgtcaggct ggccgaggca cccagctacg gccgccccat cgcgcgctac agccctgagt
     2641 cgcgcggtgc gcaggcttac caggcacttg ccgaggagct tttgggccga acggcacagg
     2701 agcagaaggc gatcatgggg gcgctatgac gactgatctc ggccgtaata cgagcggcct
     2761 cggccgtggc ctgggcgcgc tcattccgca gcgcgacgac tcggccaagc ccggagtcca
     2821 ggagctgccg atcagcgcaa tcgccagcaa cccgtatcag ccgcgccatg ccatcgagca
     2881 gtcgcagctg gaggacctca cggccagcat tcgtgagcac ggcgtgctgc aacccgttct
     2941 cgtcacacag acgcccggcg gataccgcct gattgctggc gagcgccgtc ttcgagcctc
     3001 agacgcggcc ggtttgacgc atattccagc ggtcgtccgc agcgcaacag aaagcgagca
     3061 gctggcctgg gcgctgatcg agaacattca gcgctccgat ttaaacgcca tggaagcggc
     3121 tcatgcgttc cgacgcctgg gcgatgagtt cggcctgacc cacgaagaaa tctcgcaacg
     3181 ggtcggcagg gctcgctcga cggtatcgaa cacgctccga ctgcttgacc tggcgccaga
     3241 ggtccaatct gccgtttcca gtggtgccgt gagcgagggg cacgcgcgcg ccatcggtgg
     3301 tctcagcgaa cacggcctac aggcagagat cgtcggactc gtggtcaagc gcgggctgtc
     3361 cgtgcgccag accgaagagt tgatccggcg cgcccgcgac ggtaagccga cacgctccca
     3421 gggagcggcg acaccggacg ttgagcgtct ggagcgggga ttgcgcgagg cgctatcaac
     3481 gaaggtgacg ctcaactcgt ctcgcaaggg gggacgcatc acgatcgagt actacgacga
     3541 agacgacctg gaacgcatct acgaacgcct gactggagag cggccttgac tgattcagtg
     3601 gccagccgcc cgcaggcgca gtcggccggt aatggcaggc gcggcaaagg cgctgcgagc
     3661 gactacaccg ccgagagcat ccaggtcctc gaggggctcg aggccgtccg taaacgcccg
     3721 ggcatgtata tcggctcgac ggatgcgcgc ggcctgcacc agctggtctg ggaaatcgtc
     3781 gacaactcga tcgacgaggc gatggccggc cacgccaact acatcgacct gacgatccga
     3841 gctgacggca gcgttcacaa ccgcgacaac gggcgcggcg tgcccgttgg gctccagaag
     3901 cagacgggcc gcgacgcgct cgaggtggtc cacaccgttc tccacgctgg cggcaagttc
     3961 ggcggcggtg gctacaaggt gtcgggcggg ctacatggcg tgggcgtgag cgtggtcaac
     4021 gcgctgtcca tctcactgaa ggtcgaatcg gcccgcgacg gaaagctgtg gcgccaggaa
     4081 tactcgcgtg gcaagcccac gaccaaggtc acggccgcgg gaacctcggc ggggcgcacg
     4141 ggcacgacga cagagttcat gcccgatgcc gagattttcg agacgctcga cttcaacttc
     4201 gacacgatcg cccagcggct tcgcgagtcg gcatatctga ataagggcgt ttggatccgg
     4261 ttcgtcgacc agcgcgtcac cccggcccgc gagcgcagct tcttctttga aggcggcctc
     4321 gtcagcttcg ttcggcatct caacaagggc cgcgacgtgc tgcacccgaa gccgatctac
     4381 gtcgagaagc gcgacggcag tacgcagatc gagattgcac tgcagtacaa cgacggctac
     4441 agcgagagcg tcttcgcgtt cgccaacaac atcaacaccg tcgacggcgg tacccatatc
     4501 accggcttcc gcgccgcctt gacgagctcg ctcaacgact acgcccgcaa gaacacgatc
     4561 ctcaaggacg ccgacgcgaa cctttcaggc gaggatgtcc gcgagggcct tacggccgta
     4621 atcagcgtca agctgaccga cccgcagttc gaaggccaga ccaaagccaa gctcggcaac
     4681 gccgaggtca agggtcaggt tcaggccgcg ctgacagagg gaatcttcca gtacctcgac
     4741 gagaacccgc aggacgcccg gcggatcatc gagaagagcc ttattgcggc acgtgcacgc
     4801 gaggcggcgc gcaaggcgcg cgatcttgtg atccgcaaga gcgcgctaga gggttcggca
     4861 ctccccggca agctggccga ctgccaggag cgtgatccag ctcgcagcga gctgttcatc
     4921 gtcgagggcg actcggccgg tggctcagcc aagcaggggc gcgaccgtca cttccaggcg
     4981 gttctgccga tgttcggcaa gatgctcaac gtggagaagg cgcgcatcga caaggtgctg
     5041 tcgtcggaaa agatccggcc gctgatcatc gcgctcggag ccggcatcgg cgagacgttc
     5101 gatatcgcca agctgcgcta ccacaagatc atcctgctgg cggatgcgga cgtcgacgga
     5161 gcccacattc gcaccctgtt gatgacgttc ttctaccggc acatgcctga ggtcgtcggc
     5221 agcggccacc tctacatcgc gcagccgccg ctctaccgga tcagcaccgg caaggtgact
     5281 cgctacgcga a
//
LOCUS       DATLCO010000338         3352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6385182, whole genome shotgun sequence.
ACCESSION   DATLCO010000338 DATLCO010000000
VERSION     DATLCO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6385182"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VMZ33_07865"
     CDS             complement(<1..496)
                     /locus_tag="VMZ33_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR17188.1"
                     /translation="MQRFGREVVALRGVSLDVLAGEFITLLGPSGCGKTTLLRVIAGF
                     ETPDEGRIVLEGRDMTSTPSHRRPVNMVFQRPLLFPHLDVFENVAFGLRIARTDRAEV
                     ERRVAEALRLVRLEGFERRRSHQLSGGQMQRIALARALVNRPKVLLLDEPLSALDLKI
                     RLEME"
     gene            complement(535..1344)
                     /locus_tag="VMZ33_07870"
     CDS             complement(535..1344)
                     /locus_tag="VMZ33_07870"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR17189.1"
                     /translation="MERRQQSRATALITAAAVLFLFVPLVLVVLFSFHSTGGLSFPFR
                     GFSLRWYDEIFSSHELRAATVNSLIVSTSTSLITLVAGTLAAYGLSRTASRLRGPLAL
                     LFFLPITLPGLFLGLALLVSFSRLQIKLSLTTVTVAHLVYVFPYYVLISRAALDRLDR
                     GLEEVAADLGANAMLVFRRVTLPQIWPVLLGATCLAFALSFDEFVITFFVVGEDSTLP
                     LFIWSRLRRTIDPSINAVSTLLLLSTLLLFALAFVLALRGERLRSGRTASL"
     gene            complement(1344..2186)
                     /locus_tag="VMZ33_07875"
     CDS             complement(1344..2186)
                     /locus_tag="VMZ33_07875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR17190.1"
                     /translation="MTLSMPAAAGMLLFFVAPIALFLVYSFLTAGLYSVSAPLTIDSY
                     REAVDSEFNRALARNSFLIGVTAALVTVAFAIPVAYWLRYSAGRWRMPVLFLITASMF
                     ASYLVRIYAWRSILGSNGLLNSGLERLGLVDEPLGFLLYNRFAVTVALVHIFLPYVVL
                     VVYAGFAPIPSGLLEAARDLGAGALQRWRRVILPLIAAPAASSFLFVFVLSAADYVTP
                     QFLGGANGLMLGVQIQTSFVSTGNWPLGAALSFLMLAAFVGCYAAVALALRLLKLDRI
                     VVAH"
     gene            complement(2245..>3352)
                     /locus_tag="VMZ33_07880"
     CDS             complement(2245..>3352)
                     /locus_tag="VMZ33_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR17191.1"
                     /translation="PTAATLLSACGGDDSKSSGGTTTQAAPVTVGGRLDFIAWEGYDL
                     PDVMKAWKQQHGATLKATYIGGQDEVQAKVKAAGASAGYELTTYTQGYVDLYQELDLL
                     LPLDESKLPNLDGLYPYFADDTNNYWTDADGQRIGVPWTWGSAGITYDSAKIDEMSSW
                     YDLLDPSLKGKIALPDDPQGHFALTTRILGLHPDTCPKDRLDDVSALSAKFVAQSKGV
                     SPSYGDMTTKLVSGEAVACYQGWAAMNSFAAGEGLKTVKTNVPKEGSYSFCDAWAIPR
                     GADNVDTALAWINETLSAKTNAAAAEYLVGGVTIQTSLGYLNKETKALYSYDDLDGLL
                     EQAPFNHNTPVESAEYVTFKEWNDRWQELKAGRS"
BASE COUNT          636 a         1054 c         1162 g          500 t
ORIGIN      
        1 cttccatctc cagccgaatc ttgaggtcga gagcagagag gggctcgtcg agcaggagca
       61 ccttgggacg attgacgagt gcgcgggcca gggcgattcg ctgcatctga ccgccggaga
      121 gctgatggct gcgccggcgc tcgaatccct ccagtctgac gagtcgcaag gcctccgcaa
      181 cccgacgctc gacctcggcg cgatcggtgc gggcgatgcg gagaccgaag gccacgttct
      241 cgaacacgtc caagtggggg aagagcagcg gccgctgaaa gaccatgttc acggggcgcc
      301 ggtgagacgg cgtgctcgtc atgtcacgac cctcgagaac gatcctccct tcgtcggggg
      361 tctcgaaccc cgcgatcaca cgcagcaggg tcgtcttgcc gcagccagac gggccgagca
      421 acgtgatgaa ctcccccgcc agaacgtcca acgagactcc gcggagcgcc acgacttcgc
      481 gcccgaagcg ttgcacgatc ccttcgatcc gaacctgagg ctcgccggtc tgattcacag
      541 ggatgccgtc cggccggacc tgagacgctc gccccgaagc gccaggacga aggcgagcgc
      601 gaataggagc agcgtcgaga ggagcagcag cgtcgagacc gcgttgatcg aggggtcaat
      661 cgtccggcgc aagcgcgacc agatgaacaa cgggagcgtc gagtcttccc cgacgacgaa
      721 gaacgtgatg acgaactcgt cgaagctgag cgcgaacgcg aggcaggtgg cgccgagcag
      781 caccggccag atctgaggca gcgtgacgcg ccggaacacg agcatcgcgt tcgcccccaa
      841 gtccgcggct acctcttcga gcccccggtc gagccggtcg agcgctgcgc gagagatgag
      901 gacgtagtac gggaacacgt agacgaggtg agcgaccgta acggtcgtga gcgagagctt
      961 gatctgcagg cgcgagaagg agacgagcag cgcgagcccg aggaagagcc cgggcagcgt
     1021 gatcggcagg aagaagagca gcgccagcgg cccgcgcagc cgtgacgccg tgcgagacag
     1081 gccgtacgcc gcgagcgttc ccgcaacgag cgtgatcagg gacgtcgatg ttgagacgat
     1141 cagactgttc acggtcgccg cgcggagctc gtgcgaggag aagatctcgt cgtaccagcg
     1201 aagggagaaa ccacggaagg ggaatgacag ccctccggtc gagtggaatg agaacaggac
     1261 gacgaggacg agcgggacga acaggaacaa gaccgcagcg gcggtgatga gggctgttgc
     1321 ccgactctgc tgacgccgtt ccatcagtga gcgacgacga tccggtccag tttcagcagc
     1381 cgcagggcga gcgcaacggc cgcatagcag ccgacgaacg ccgcgagcat caggaacgac
     1441 agcgcggccc ccagcggcca gttgcccgtg gagacgaagc tggtctggat ctgcacgccg
     1501 agcatgaggc cgttcgcacc gccgagaaac tgtggcgtca cgtagtcggc agccgagagg
     1561 acgaagacga acaggaacga gctggccgcc ggtgccgcga tcaggggcag gatcacccgc
     1621 cgccagcgtt ggagcgctcc ggccccgagg tcgcgcgcgg cctcgagcag ccccgacggg
     1681 atgggcgcaa agccggcgta aaccaccagc acgacatagg gcaggaagat gtgcacgaga
     1741 gcgaccgtca ccgcgaagcg gttgtagagg agaaacccaa gcggctcatc gaccaggccc
     1801 agtctctcta ggccgctgtt gagcagcccg ttcgatccga ggatgctgcg ccaggcatag
     1861 atcctgacca aatagctggc gaacatcgac gcggtgatca ggaagaggac gggcatgcgc
     1921 cagcgccccg cgctgtagcg cagccagtag gcgacgggga tcgcgaaggc gactgtcacg
     1981 agcgcagcag tcaccccgat caggaacgaa ttgcgggcca gagcccggtt gaattccgag
     2041 tcgacagcct cgcgatagct gtcgatcgtc agcggagcgg agacgctgta gagccctgcg
     2101 gtcaggaagc tgtagacgag aaacagcgca atcggggcga cgaagaagag cagcatgccc
     2161 gcggcagcgg gcatgctgag cgtcattggt cctgcgcgtc tgacgaagcc gcgagtcgcc
     2221 ggcttcaccg gctgctgcgg cgtctcaact gcggcccgcc ttgagctcct gccaccggtc
     2281 gttccactcc ttgaacgtga cgtactcggc cgactcgacc ggcgtgttgt ggttgaacgg
     2341 cgcctgctcg agcaggccgt ccagatcgtc gtagctgtag agcgccttgg tctccttgtt
     2401 gaggtaaccg agggaggtct ggatcgtgac gcccccgacc aggtactcgg ccgccgctgc
     2461 gttggtcttg gcgctcagcg tctcgttgat ccaggcgagc gcggtatcca cgttgtcggc
     2521 tccccggggg atcgcccagg cgtcgcagaa cgagtagctt ccttccttgg ggacgttcgt
     2581 cttgaccgtc ttgaggccct cgccggcggc gaagctgttc atcgcggccc agccctggta
     2641 gcaggcgacc gcttcgccgg agacgagctt cgtggtcatg tcgccgtacg atggcgagac
     2701 gcccttcgac tgcgcgacga acttggcaga gagcgcgctg acgtcgtcca gtcggtcctt
     2761 cgggcacgtg tccgggtgca gcccaaggat ccgcgtcgtc agggcgaagt ggccctgcgg
     2821 gtcgtccggg agggcgatct tgcccttcag cgaggggtcg agcaggtcat accaggagct
     2881 catctcgtcg atcttcgccg agtcgtaggt gatccccgcc gagccccagg tccagggcac
     2941 gccgatcctc tggccgtccg catcggtcca gtagttgttc gtgtcgtcgg cgaagtacgg
     3001 gtagagcccg tcgaggttcg gcagcttcga ctcgtcgagc ggaaggagca ggtcgagctc
     3061 ctggtagagg tcgacgtaac cctgcgtata ggtcgtgagc tcgtagcccg ccgaagcccc
     3121 ggccgccttg accttggcct ggacctcgtc ctggccgccg atgtacgtcg ccttgagggt
     3181 ggccccgtgc tgctgcttcc aggccttcat cacgtcgggc aggtcgtatc cctcccacgc
     3241 gatgaaatcg agccggcctc cgaccgtgac cggggcggcc tgcgtcgtcg tcccgccgga
     3301 gctctttgag tcatcgccgc cgcacgcgga cagcagcgtg gccgcggttg gg
//
LOCUS       DATLCO010000339         2965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10119938, whole genome shotgun sequence.
ACCESSION   DATLCO010000339 DATLCO010000000
VERSION     DATLCO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10119938"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VMZ33_07885"
     CDS             complement(<1..345)
                     /locus_tag="VMZ33_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HUR17192.1"
                     /translation="MTSLAVSASGDYRVVLAAGALPPCRYDDVITDHTGYKQWNKTLI
                     DSMFRLPKSYMPPPALVSTDQANVRGNEKLRQIVIADLKALVRAARLANVRLMVVSGY
                     RSWAEQKYLYDRE"
     gene            488..2074
                     /locus_tag="VMZ33_07890"
     CDS             488..2074
                     /locus_tag="VMZ33_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUR17193.1"
                     /translation="MNSRRWHWAGAGAAAAAVAIGVGEISAALLGGTSIIAAVGTLVI
                     ALQPPGGKDLAVQLFGTNDKLALEIGAALGGILVGALLGVFSRKDVRIGVIGFVAFGA
                     FAFYLLQSDPLSGMVAALASAAAATAAGSVMLTWLTASLRKPATAVTAAVTTSAVGEA
                     PLAATPRPSIDRRGFLALSAMFVAVGGVLAFIGRYLTSQLPAVVADPSSIPVARQTVP
                     PVAGGTDFGVAGLTPIVVPNDDFYRIDTRLDVPRVDAATWSLRVHGLVDREVSLTYEQ
                     LLAMPLVERYVTIACVSNPVGGRLVGNAKWTGVPLTTVLEQAGVKPEASQLVGRSFDG
                     WTAGFPTEQLRGAGSEALIAVAMNGVPLPPAHGFPARLIVPGLYGYVSATKWITEIEL
                     TTLESFDAYWVPLGWSKFGPILTQSRVELPRAGQQLTAGPVQFAGVAWAPTRGINKVE
                     VIVDATPEGAGTWHQAELSQPLSSATWVQWRVTLDVPAGEHRIAVRATDGTGETQTSD
                     ITPPPPDGARGYHQVSFGAR"
     gene            complement(2094..2603)
                     /locus_tag="VMZ33_07895"
     CDS             complement(2094..2603)
                     /locus_tag="VMZ33_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17194.1"
                     /translation="MKADGAGFELDFVITAQHLVDFLRLRQRTLNRLGSFVALGLIVA
                     GLYFAISGDRVLGAFEVIVGVLMLVTSQTTVFDAWRVKRAGRTVIGTRAHLTVEDDGM
                     TIQNADMSNHIEWKSITELRINDRIVIPMRGRLPVGWLPTDAFASLAEREAAIAYMRD
                     RITDADTRT"
     gene            complement(2614..>2965)
                     /locus_tag="VMZ33_07900"
     CDS             complement(2614..>2965)
                     /locus_tag="VMZ33_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR17195.1"
                     /translation="ANEFPDRLSGGQQQRAAIVRAMAMRPEILLLDEITAALDPELVA
                     EVLDVIRELADSGMTLVLATHEMGFARDVADRICFLDAGRILEQGPPAQILSDPREER
                     TQRFLQRVIRAGRL"
BASE COUNT          487 a          978 c          992 g          508 t
ORIGIN      
        1 ctcgcggtcg tagaggtatt tctgttccgc ccagctccga tatccggaga cgaccatcaa
       61 acgcacgttg gccaggcgcg cggctcggac gagagccttg aggtcggcga tgacaatttg
      121 acggagcttc tcgttgccgc ggacgttcgc ctggtcggtc gagacgaggg cgggtggcgg
      181 catgtacgac ttcggaagcc ggaacatgga gtcgatcagc gtcttgttcc attgcttgta
      241 gccggtgtga tcggtaatga cgtcgtcata acggcatggg ggcagcgcgc cagcggcaag
      301 cacgacgcgg taatcgccac tcgcgctgac agccaacgat gtcacggcca aggcgaagga
      361 cagcactaaa aacctgagtc tgataacggt cacggccttc gaatcgtgac gggccagacg
      421 cggaaccgtc aagttgacca acgtcggtgt gatcccgcga gccgagccgt gcgcataacc
      481 tgacgacatg aactcccgcc gttggcactg ggcaggggct ggcgccgccg cggccgccgt
      541 tgcgatcggc gtgggcgaga tcagtgctgc gctcctcggc ggcacttcga tcatcgccgc
      601 cgtcggcacg cttgtgatcg cgctccaacc acctggcggc aaggacctcg ccgttcagct
      661 gtttggtaca aacgacaagc tggcgctgga gataggagcg gcgctgggcg gcatcctcgt
      721 cggcgcccta ctgggcgtgt tctcccgaaa agacgttcga atcggcgtca tcggcttcgt
      781 cgcatttggc gcgttcgcgt tctacctgct tcagagcgat ccgctgtcgg gcatggtcgc
      841 ggccctggcg agcgcagcag cggcaaccgc tgctgggtcg gtgatgctga cctggctgac
      901 ggcctcgctc cgaaaaccag ccactgccgt caccgctgcc gtcaccacct cagccgtggg
      961 cgaggctcca ctggctgcga cgcccagacc ttccatcgac cgtcgcggct tcctcgcgct
     1021 gagcgccatg ttcgtggccg tcggcggcgt gctggctttc atcggccgat atttgacctc
     1081 gcaactgcct gccgtggtcg ccgatcccag ctccatcccc gtggcgcgtc aaacagtccc
     1141 gcccgtagct ggtggcacgg atttcggcgt ggcgggcctc acgccgatcg tcgtcccgaa
     1201 cgacgatttc tatcggatcg atacccgcct cgacgtcccg cgcgtcgacg cggccacgtg
     1261 gagcctgcgt gtccacggcc tggtggatcg ggaagtgtcg ctgacctacg agcaactcct
     1321 ggcgatgccg ctcgtggagc ggtacgtgac gatcgcgtgt gtctccaacc cggtcggtgg
     1381 ccggctggtc ggcaatgcaa agtggactgg cgtgcccctg acgacggtgc tggagcaggc
     1441 cggtgtgaag cccgaggcga gccagctcgt cggccgttcg ttcgatggct ggaccgccgg
     1501 gttcccgacg gagcaattgc gcggtgctgg cagcgaggcg ctgatcgccg tcgcgatgaa
     1561 cggcgtgccg ctgccgccgg cgcacggctt cccggcgcgg ctcatcgtgc ccggccttta
     1621 cggctacgtg tcggcgacga agtggatcac ggagatcgag ctgacgaccc tcgaatcttt
     1681 cgatgcctac tgggtaccac tcggttggtc gaagtttggg ccgattctca cgcagtcgcg
     1741 cgtcgagcta ccgcgcgcag gccagcaact cacggcgggc cccgtccagt tcgctggcgt
     1801 cgcatgggca ccgacacgtg gtatcaacaa ggtcgaggtg atcgtcgacg ccactcccga
     1861 gggcgccgga acctggcacc aggcggagct atcgcagccc ctgtcctcag ccacctgggt
     1921 tcaatggcgg gtgaccctgg acgtgcccgc cggggagcat cgcatcgccg ttcgggccac
     1981 ggacggcaca ggcgagacgc agacctcgga catcacgcca ccgccgcccg acggggcacg
     2041 cggctatcac caggtgagct tcggcgcgcg ctagccctcc aagcgctccc cacttaggtg
     2101 cgggtgtctg cgtccgtgat gcggtccctc atgtaggcga tagccgcctc gcgctcagcg
     2161 agcgaggcaa aggcgtcggt cggcaaccag cccacgggta gccggccgcg catcgggatg
     2221 acgatgcgat cgttgatccg gagctcggta atggacttcc actcgatgtg attcgacatg
     2281 tcagcgttct ggatcgtcat gccgtcgtcc tcaacggtga ggtgcgcacg cgtgccgatc
     2341 actgtccgtc ccgcgcgttt gacacgccac gcatcgaaga ccgtcgtctg cgaggtgacg
     2401 agcatcagga cgccgacaat gacctcgaac gcgccgagca cccggtcgcc gctgatcgcg
     2461 aagtagagcc cggcgacgat gagaccgagc gcgacgaagg acccgagccg gttcaaggtt
     2521 cgctgccgaa gccgcaggaa gtcgaccaga tgctgggcgg taatgacgaa gtcgagctcg
     2581 aacccggcgc cgtcggcctt catgcccgaa aggttagagc cggccggcac ggatcactcg
     2641 ctgcaggaac cgctgcgttc gctcctcgcg cgggtcgctc aggatctgcg cgggcgggcc
     2701 ctgctcgagg attcgtccgg cgtcgagaaa gcagatgcgg tcggcgacat cgcgcgcgaa
     2761 ccccatctcg tgcgtcgcga ggacgagcgt catacccgag tccgcgagct cgcggatgac
     2821 gtcgagcacc tccgccacca gctcgggatc gagcgcggcc gtgatctcgt cgagcaacag
     2881 gatttcgggc cgcatcgcca tcgcccggac gatggccgcc cgctgctgct ggccgccgga
     2941 gagtcggtcg ggaaactcgt tcgcc
//
LOCUS       DATLCO010000340         5659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7883452, whole genome shotgun sequence.
ACCESSION   DATLCO010000340 DATLCO010000000
VERSION     DATLCO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7883452"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1143)
                     /locus_tag="VMZ33_07905"
     CDS             complement(169..1143)
                     /locus_tag="VMZ33_07905"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HUR17196.1"
                     /translation="MLTQKAARSPLPATGVRPADDSIVAWFEGKFVPLSEARIPVMTH
                     GFLYGTATFEGIRAYWNEEQEQLYLLKALDHFKRMQRSAKVLLMKLPMPPEELVALTV
                     DLLRRNGFRQDTYVRPTLYKSTEAIGVRLHNLEEQLVIFSVPFGEYIAIDRGISAQVV
                     SWRRNSDQALPARAKIVGGYANSAFSKSEAVLNGYDEAIVLTMDGHVSEGSAENLFIV
                     RDGKLVTPSVTDDILEGITRTALMQLAREEMGIETIQRPIDRSELYNAEEVFLCGTGA
                     QLSPVVEIDHRSIGNGEVGPITRQLATLYFDAVRGRSPAYSDWLTPIY"
     gene            complement(1219..2280)
                     /locus_tag="VMZ33_07910"
     CDS             complement(1219..2280)
                     /locus_tag="VMZ33_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17197.1"
                     /translation="MEYLELAIALVVILAAAELFTNGIEWFGHKLNLAEGAVGSVLAA
                     VGTALPETMIPLVAILFAGSEGAEGSGHEIGVGAILGAPFMLATLAMFVTGLGVVGWA
                     RRRRAGREMPVDTHVLGMDIRYFFVAYSIAVGAAFVPPDLHWLRYVAVVAVIGIYAWY
                     VKAHFQADTTIDAVDLAPLRFRKLDPRAHKTNPETPRLRIVNAQVLVALGLIIGGAFV
                     FVDGVEHVATSLALDPALLALIIAPIATELPEKFNSLIWVRQGKDTLAMGNITGAMVF
                     QSCIPTAIGIVLAADAWTVSGDTILSFASAGIAFAATALIFIPMARRGTLNGVHLLIG
                     GAFYLAYAILVIGKLAGAF"
     gene            complement(2294..2557)
                     /gene="rpsR"
                     /locus_tag="VMZ33_07915"
     CDS             complement(2294..2557)
                     /gene="rpsR"
                     /locus_tag="VMZ33_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HUR17198.1"
                     /translation="MPPCRTKKRDEYRDRRRRKVCAFCADKTAMIDYKEINRLRRYLS
                     ERAKIEPRRKTGTCAEHQRQLAVALKRARHVALLPYAPEHLRA"
     gene            complement(2594..3055)
                     /locus_tag="VMZ33_07920"
     CDS             complement(2594..3055)
                     /locus_tag="VMZ33_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HUR17199.1"
                     /translation="MSLNKCMIIGNLGRDPEMRYTPSGQAVTQFTVAVNRNYKDAQGE
                     RQEETEWFRVVAWGQQAEFAAEYLRKGNKVYVEGRIQTRQWEGQDGQKRYTTELVANT
                     IQNMERRPRDDQGDGGSQLPPEPRGARAPAARQGGGGQEQPEPETYDDLPF"
     gene            complement(3065..3511)
                     /gene="rpsF"
                     /locus_tag="VMZ33_07925"
     CDS             complement(3065..3511)
                     /gene="rpsF"
                     /locus_tag="VMZ33_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HUR17200.1"
                     /translation="MRRYELMLVMRPDSTDEQVDALVDRATRSVTEAGGQVVKRSPWG
                     RRRLAYPIGQFREGSYHIVLFDSPATAVLEMERGLNISEDVLRHMVTRVERPLSRRAA
                     AGDDIDLLLDDDTLAGPPPEVDEEEIDDQFIDESESEAAPAAVERP"
     gene            complement(3645..4382)
                     /locus_tag="VMZ33_07930"
     CDS             complement(3645..4382)
                     /locus_tag="VMZ33_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17201.1"
                     /translation="MRVSVLMAVVMLLAAAAPTSAAPRAGGVGDALTGGSGGAAGSVG
                     ASGSPGACNDNKYTKLNPAARWISTLEWRFRHSSVPAGLNSGEVLALIKKSFRNVVNA
                     RNDCGLADRVSATHQYLGTTSRRPNVTRAGGCGAADGYNTVGFGPLDGYYSGYTCIWW
                     IGAEVVEADVRLDTDTDWALASNGCFGELMMEALVTHEVGHAYNLGHVAENNHGRLTM
                     SVYIDQLCGNQEATLGLGDVLGLESMY"
     gene            complement(4466..5107)
                     /locus_tag="VMZ33_07935"
     CDS             complement(4466..5107)
                     /locus_tag="VMZ33_07935"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HUR17202.1"
                     /translation="MAQPDRSGLIRNVLIISVAVAALAIIGFLFLQPPGPTYACTTEL
                     TPPAVQEDSSGLGFAAPDLGNAHVAPPGSKITYGYCPPASGGHWNAENRGPIPARVYP
                     PNEERPPGGWVHNLEHGYVAILYRCSSGQLGSGDCATADEMTAMRAWFDARPSTDTCG
                     QQALVARFDSMTTRFAILAWDRLLMLDTLDLAQAETFADQWTDNPALPEAGVC"
BASE COUNT          970 a         1826 c         1855 g         1008 t
ORIGIN      
        1 ttcggcgctc atcccaaaga gccagaatct cgtgctcgat ggcaggaaaa tccggctggc
       61 ggggtacggg tttgaacggg ctcgtcaccc gctgctcgat ggacatgggc cggtaagcat
      121 agccgctacc cggctctcag cggaccgccg cggcgtcggc cgcgggccct aatagatcgg
      181 ggtgagccag tcgctgtaag cgggcgaacg cccgcggacg gcgtcgaagt agagcgtcgc
      241 cagctggcgt gtaatcggtc cgacctcacc gttgccgatg ctgcggtggt cgatctccac
      301 gaccggcgaa agctgcgcgc ccgtgccaca caggaagacc tcttcggcgt tgtagagctc
      361 cgagcggtcg atcggccgct ggatcgtctc gatgcccatc tcctcgcgcg cgagctgcat
      421 gagcgcggtc cgagtgatgc cttcgaggat gtcgtcggtg acactcggag tgacgagctt
      481 gccatcgcgg acgatgaaca ggttctcggc actcccctcc gacacgtgac cgtccatggt
      541 caggacgatg gcctcgtcat agccgttgag gactgcttca ctcttcgaga aggcgctgtt
      601 cgcgtagccg cccacgatct tggcgcgcgc cggcagggcc tggtcagagt tacggcgcca
      661 cgacacgacc tgggcgctga tgccgcgatc gatcgcgatg tactcgccga acggcacgga
      721 gaagatgacc agctgctctt cgaggttgtg caggcggacg ccaattgcct cggtcgactt
      781 gtagagcgtc ggtcgcacgt acgtgtcctg gcgaaagcca ttccgtcgca gaaggtcgac
      841 ggtcagcgct accagctctt cgggtggcat cggcagcttc atcagcagca ccttcgcgga
      901 gcgctgcatg cgcttgaagt ggtcgagggc cttgagcagg tacagctgtt cctgctcttc
      961 attccagtag gcgcgtatgc cttcgaacgt ggcagtgcca tacaggaacc cgtgagtcat
     1021 cacgggaatg cgtgcctccg aaagcggcac gaacttcccc tcgaaccaag cgacgatgga
     1081 gtcgtcggct ggacggacgc cggtcgcggg gagtggtgac cgggctgcct tctgcgtcag
     1141 cacggcccgg agtatacccc tcggccaccc cgccgcgggg tgccacgcct cacggctttc
     1201 atgacaaagc ccgtccgatc agaacgcacc agccaacttg ccaatgacca ggatcgcgta
     1261 ggcaaggtag aaagcgccgc cgatcaggag gtgcaccccg ttcaacgttc cgcggcgggc
     1321 catcgggatg aagatcaacg ccgtcgcggc gaaggcgatg ccggccgagg cgaatgacag
     1381 gatggtgtcc ccggataccg tccaggcgtc cgcggcgagg acgatgccga tagccgtcgg
     1441 aatgcacgac tggaagacca tcgcgccggt gatgttgccc atcgcgaggg tgtccttgcc
     1501 ctggcggacc cagatcaacg agttgaattt ctcgggcagc tccgtcgcga tcggggcaat
     1561 gatcagggcc agcaaggccg ggtcgagggc cagcgacgtc gccacgtgct ccacgccgtc
     1621 aacgaagaca aacgcgccac cgatgatcag cccgagagcc accagcacct gggcattgac
     1681 gatgcgcagt cgcggggttt caggattggt tttgtgagcg cgcggatcca gcttgcggaa
     1741 gcgcagaggc gccaggtcga cggcgtcgat cgtcgtatca gcctggaagt gcgcctttac
     1801 gtaccaagcg tagatgccga tgaccgccac aacggccacg taacgaagcc aatgaaggtc
     1861 gggcggcacg aacgccgccc cgacggcgat tgagtaggcg acgaagaagt accgaatgtc
     1921 catgcccagc acgtgcgtgt cgacgggcat ctcgcggccg gctcgacggc gacgcgccca
     1981 cccgacgacg cccagacccg tcacgaacat ggccagcgtc gcgagcatga atggcgcgcc
     2041 caggatggcc ccgacgccga tctcgtggcc tgagccttcc gcgccttcgc ttccggcgaa
     2101 gaggatggcg accagcggga tcatcgtttc cggtagcgcc gtgccgaccg cggccaggac
     2161 cgatccgacg gcaccctcgg ccaaattcag cttgtggccg aaccactcga ttccgttggt
     2221 gaaaagctca gccgccgcca ggatgacgac gagggcgatt gcgagctcca gatactccat
     2281 gacgccgctg cggctaggcc cgcaggtgct ccggtgcgta aggcagcaac gcgacgtgcc
     2341 gtgcacgctt cagcgcaact gccagctgac gctggtgctc ggcgcaggtg cccgtcttgc
     2401 gacgtggctc gatcttcgcc cgctcagaca ggtagcgacg caggcgattg atctccttgt
     2461 agtcgatcat cgcggtcttg tcggcgcaga aggcgcatac cttccggcgg cgccggtcgc
     2521 gatactcatc gcgcttctta gtacggcatg gcggcatttg tcggcggtcc tttctcgatc
     2581 ggaagtcggc tgcctagaaa ggcaggtcgt cgtaggtttc gggttccggc tgctcctggc
     2641 cgccgccgcc ctgtcgggca gcaggggcgc gggcgccgcg cggctctggc ggaagctggc
     2701 taccaccgtc gccctgatcg tcgcgaggac gacgttccat gttctggatc gtgttggcaa
     2761 cgagctcggt cgtgtaccgc ttctggccgt cctgcccctc ccactgtcgg gtctggatcc
     2821 ggccctcgac atagaccttg ttgcccttgc gcaggtactc agcggcgaat tcggcctgct
     2881 ggccccacgc aacgacgcgg aaccactccg tctcctcttg ccgctcgccc tgagcgtcct
     2941 tgtagttcct gttcacggcg accgtgaact gggtcacggc ctgcccgctc ggcgtgtagc
     3001 gcatctcggg atcgcggccg aggttgccga tgatcatgca cttgttcaat gacatggcag
     3061 cctcctacgg gcgctcgaca gcggcgggcg cggcctcgct ctctgactcg tcgatgaact
     3121 ggtcgtcgat ctcttcttca tcgacttcag gcgggggtcc cgccagagtg tcgtcgtcga
     3181 gcaacaggtc aatgtcgtcg ccggctgctg cgcggcgtga tagcggccgc tcgacacggg
     3241 tcaccatgtg gcgcagcaca tcctcgctga tgttcaggcc acgctccatc tcgagcaccg
     3301 ccgtcgctgg cgagtcgaac agaactatgt ggtacgagcc ctcgcggaac tgacctatcg
     3361 gataggcgag acggcgccgg ccccacgggc tgcgcttgac tacctggcca ccggcttcgg
     3421 tgacggaacg cgtcgcgcgg tcgaccagtg catccacctg ctcatcggtc gaatcgggac
     3481 gcatcacgag catgagctca taacgtcgca tgtatgtgtc tccttccctt ggggatgccc
     3541 cgctgcagag ttcgtttgag ctcggcggag ctgaaaggtg gtcccggaag tatagacgag
     3601 ggcggcccaa ccaccgccga ccggacctgg cgcggggtcg acctctagta catcgactcc
     3661 aggccgagga cgtcgccgag gcccagcgtt gcctcctggt tgccgcatag ctggtcgatg
     3721 tagacgctca tcgtgagccg gccgtggttg ttctccgcga cgtggccgag gttgtaggcg
     3781 tggcccacct cgtgtgtcac gagcgcttcc atcatcagtt cgccgaagca gccgttggag
     3841 gcaagagccc aatcggtgtc ggtatccagg cgcacgtcgg cctcgacgac ctcagcgccg
     3901 atccaccaga tgcaggtgta gccgctgtag taaccgtcga gtggtccgaa cccgaccgtg
     3961 ttgtacccgt cggccgcgcc acagccgccg gcgcgggtga cgttgggccg acgggaggtc
     4021 gtgcccaggt actggtgtgt ggcgctcacg cgatcggcga ggccgcagtc attgcgcgcg
     4081 ttgacgacgt tacggaacga tttcttgatg agcgcaagaa cctcgcccga gttgaggccg
     4141 gcaggaaccg acgaatggcg gaagcgccac tcgagcgtcg atatccagcg cgccgccgga
     4201 ttgagctttg tgtacttgtt gtcgttgcat gcgcccggcg agccactcgc ccccaccgat
     4261 cctgcggcgc cgccagaccc tcccgtcagg gcgtcgccca cgccgccggc gcgtggcgct
     4321 gcagacgtcg gcgccgcggc ggccaggagc atcacgacag ccatcagaac cgaaacgcgc
     4381 acgacgcacc cctccttgaa cccgtcacgt ccgacggttg caacgcggag agtctaggct
     4441 gttgccgctc cgggaacgct gctgctcaac agactccggc ctcgggcagc gctgggttgt
     4501 ccgtccattg gtctgcgaac gtctcggcct gcgccagatc caacgtgtcg agcatcagga
     4561 ggcggtccca cgcgaggatg gcaaagcgcg tggtcattga gtcaaagcga gcaaccagcg
     4621 cttgctgtcc acaggtgtcg gtgctgggcc gcgcatcaaa ccaggcacgc atggctgtca
     4681 tctcgtcggc agtcgcgcag tcacctgaac caagctgacc gcttgagcag cggtacagga
     4741 ttgccacgta gccgtgctcg aggttgtgta cccagccgcc aggcggccgc tcttcattgg
     4801 gcgggtacac acgggctggg ataggcccgc gattttcggc gttccagtgc ccgcctgacg
     4861 ccggtgggca atagccatac gtgatctttg atccgggcgg cgcgacgtgg gcattgccga
     4921 ggtccggcgc ggcgaatccc agaccgctcg agtcctcctg aacggcgggt ggcgtcaact
     4981 cggtcgtgca ggcgtatgtc gggccaggag gctgaaggaa caggaaacca atgattgcca
     5041 atgctgcaac ggcgacgctg atgatcagca cgttgcggat caggccggag cgatctggct
     5101 gtgccaagga cgcggacgtc cgggttcccc gggtgggcgg cgtgcggctg gcgcttttcc
     5161 gctcgcggcg agtcgtcacg ttaccccttg agtatgttgg tgccgaaggt gagattcgaa
     5221 ctcacacgcc cttgcgggcc gcggattttc agtccgagtc atcacgtccc gcaggagtcc
     5281 gccgagtacc ctgacacgct cagattgtcg ctcaggcgct cccgtccggc cgtgcctgtt
     5341 cggcgcggcg gccaggtcgt ccaggagatg atacgaatcg cgcccgtcga cccacgaacc
     5401 agccccgtgg agtggtgacg tggaagctgt gctcaggcgt cctattggtc cctcacgctc
     5461 ggctacgccc gcaacgtaga agagccgggg tcatttgcga gactcctgct tggtctgaag
     5521 gccaaaacta gccgcacaag cccgcggcgc catggcttac atcgatggac acggacatcc
     5581 atggcgacag cggcgggtct tttgagatgg aggcctcgct catcgcacgt ggagctatcc
     5641 tcttgctgcc ggtccagcc
//
LOCUS       DATLCO010000341         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8734428, whole genome shotgun sequence.
ACCESSION   DATLCO010000341 DATLCO010000000
VERSION     DATLCO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8734428"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VMZ33_07940"
     CDS             complement(<1..215)
                     /locus_tag="VMZ33_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17203.1"
                     /translation="MIDRSPDAGLEPRVAAPASGRPLLVELYEAAVAGAAPAPITTEA
                     LRDFDTQPGQQIWLYVFGKAAHPMAAAA"
     gene            complement(229..549)
                     /locus_tag="VMZ33_07945"
     CDS             complement(229..549)
                     /locus_tag="VMZ33_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HUR17204.1"
                     /translation="MEAYRDQYAKLFNNGRNVVVLAVSVDPDTTLASWARDLQTPLLF
                     ASDVGQTVGRKYGSIRGGVDNRNLFVIDSSGRIAHRMIPFNELAQTSYDELSAAVKKT
                     LPTK"
     gene            complement(559..804)
                     /locus_tag="VMZ33_07950"
     CDS             complement(559..804)
                     /locus_tag="VMZ33_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17205.1"
                     /translation="MFSNTRSRFALAALLAVAASAAGAQQPASAAGPPPEVGTMAPDF
                     ALPGATRYGLLRDSVRLSDFRGKTVVLGFFYKARTKG"
     gene            1037..1540
                     /locus_tag="VMZ33_07955"
     CDS             1037..1540
                     /locus_tag="VMZ33_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HUR17206.1"
                     /translation="MRKLGMTLSLAGLALIGACSRGNDARSDDALKNDLALAAQAQPY
                     QAQQFVSPMESQYGMPYGAQQYNAAARSPYYAAAPVYSPAPVYRAPARTTTRRSSSSG
                     SSGTYYPAPARQPVRHTKRDAAIGAVAGAVIGATASRDKVKGGLIGAAAGGILGGIIG
                     HTVDVQK"
     gene            1712..>2085
                     /locus_tag="VMZ33_07960"
     CDS             1712..>2085
                     /locus_tag="VMZ33_07960"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HUR17207.1"
                     /translation="MSDDGTTRANESERAGSERRRRRTVANLAFGAIKAQHAAYRSPL
                     ERFADRLNSFASSTPFLVCHAVWFGIWISWNTGLLGLRPFDPFPFGLLTMVVSLEAIF
                     LSIFVLMAQQRESAIAELREELG"
BASE COUNT          356 a          669 c          723 g          337 t
ORIGIN      
        1 gccgccgcgg ccatcggatg cgccgccttg ccgaagacat acagccagat ctgctggcca
       61 ggttgcgtgt cgaagtcgcg gagcgcttcc gtggtgatgg gcgccggtgc ggcgccggcg
      121 accgccgcct cgtacagttc gacgagcagt gggcgaccgc tcgctggagc agcgacccga
      181 ggttcgaggc ctgcgtcagg cgagcggtcg atcaccgtgg aagttcagtc acttggtcgg
      241 gagcgtcttc ttgactgcgg ctgagagctc gtcgtacgag gtctgcgcga gctcgttgaa
      301 tgggatcatg cggtgcgcga ttcgtccgct cgagtcgatg acgaacaggt tgcggttgtc
      361 cacgccaccg cggatggagc cgtacttccg gccgaccgtc tgtccgacgt cgctggcgaa
      421 gagcagtggg gtctgaaggt ctcgcgccca ggacgcgaga gtcgtgtcgg ggtccacact
      481 cacggcaagg acgacgacgt tccgtccatt gttgaagagc ttggcgtact gatcacggta
      541 cgcttccatt tggatcgttc agcccttggt ccgtgccttg tagaagaagc cgagcacgac
      601 cgtcttccca cggaagtcgg acagccggac ggagtcgcgg aggagaccgt agcgggtggc
      661 gcctggaaga gcgaagtcgg gcgccatggt gccgacttcc ggaggtggac cggcggcgga
      721 cgccggctgc tgggcgcctg ctgcagaggc cgcaaccgcg agcagggcgg ccagcgcgaa
      781 gcgcgatcga gtattcgaga acatcgattc gtctccaggg gtatgtgaag cgggcgccaa
      841 catgcggagc cgcgaagcct actgcaatgc tggcgccgga ccaggcggtg tccgccggag
      901 tggacgattc gtctcgccgg atggacgcca gaggcgccaa aggtgtccgg gaggcgcccc
      961 tgccgcactc gagggctggc ctagggcctg cctaagtagg gtgggtagaa agaccgcaca
     1021 tgcaacggag gattcaatgc gcaaacttgg gatgacactg tcgctggccg gactcgccct
     1081 gatcggggcc tgcagccgcg gcaacgacgc gcgttccgac gacgcgctga agaacgacct
     1141 tgcactggcc gcccaagcgc agccgtacca ggcgcagcag ttcgtgtcgc cgatggagtc
     1201 gcaatacggg atgccctatg gcgcccagca atacaacgca gcggcgcgct cgccgtacta
     1261 tgcggctgcg cccgtctaca gcccggcccc cgtctatcgc gcaccggccc gcaccaccac
     1321 gcggcgcagc agcagcagcg gctcctcggg gacgtactac ccggcgccag ctcgccagcc
     1381 ggttcgtcat acgaagcgtg acgcggcgat cggagcagtg gcgggtgccg tgatcggcgc
     1441 caccgccagc cgcgacaagg tcaagggtgg cctcatcggc gccgcggcgg gcggtatcct
     1501 gggtgggatc atcggccaca ccgtcgacgt gcagaagtag ccgccatcga gctgaccaga
     1561 tcagcagccg aaattctgcg ctacgcgcat gcgacactgc ccacaccaga acggtgtggg
     1621 cagtgtcgct tcttgcatcg ccgcgtcgcg gaggcgaatg tgagcaccat gacgggctca
     1681 aggggcatgc ttctcgctag cgggggcgac catgagtgac gacggcacga ccagggcgaa
     1741 cgagagcgag cgcgccggat cagagcggcg ccggcgacgc accgtcgcca acctggcgtt
     1801 cggcgccatc aaggcgcaac acgccgccta tcgctcgccg ctcgagcggt tcgccgaccg
     1861 cctcaacagc ttcgccagct cgacgccatt tctggtctgc cacgcggtgt ggttcgggat
     1921 ctggatctcg tggaacacag ggctcctcgg ccttcgtccg ttcgatccat ttcccttcgg
     1981 ccttctcacg atggtcgtat cgctcgaggc gatcttcctc tcgatcttcg tgctgatggc
     2041 ccagcagcgc gagtcggcga tcgcggagct ccgcgaggag ctcgg
//
LOCUS       DATLCO010000342         3850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7900249, whole genome shotgun sequence.
ACCESSION   DATLCO010000342 DATLCO010000000
VERSION     DATLCO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7900249"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VMZ33_07965"
     CDS             <1..512
                     /locus_tag="VMZ33_07965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUR17208.1"
                     /translation="TLPPCMLRPRFGALCALLVSLILVSPASAVPDGPLDVYLEHLEP
                     LTLEWPAQGTVTDGFGLRWGRPHLGIDVGILTSLDVRAARSGTVTEVGWLTGYEGYGN
                     VVMVDVGSGYSVLYAHLSATSVVPGQWVDDGDPIGTAGCTGSCTGTHLHFELRSGGVP
                     VDPFPYLNK"
     gene            571..1047
                     /locus_tag="VMZ33_07970"
     CDS             571..1047
                     /locus_tag="VMZ33_07970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUR17209.1"
                     /translation="MTRTLPQRSALVVLLLSLVVAPAASARTDSAARLQMAWPASGTV
                     TSHYGEWRGSRRHDGIDIGMLRTLRLRAVTAGVVRKTGYVPGYAGYGNVVVLALPGGY
                     SALYAHLSQVGVKPGQRVRKGQRIGLAGCTGSCSGTHLHFEVHRKGRSVNPLRFLG"
     gene            1060..1132
                     /locus_tag="VMZ33_07975"
     tRNA            1060..1132
                     /locus_tag="VMZ33_07975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1093..1095,aa:Val,seq:gac)
     gene            complement(1189..2604)
                     /locus_tag="VMZ33_07980"
     CDS             complement(1189..2604)
                     /locus_tag="VMZ33_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR17210.1"
                     /translation="MADVADGVVRPSSELTRGDLLKRGTAAAFTIGMFGGLTDRALGF
                     YGPLKYANRQLSGQLRILQWAHFVPAYDQWLDNTYVKRWGEANDVEVKIDHINNALLY
                     STGASQVAAQSGHDLFWFISPPSTFQKQVVPVTDIVQEVSRKLGPMSRVARKSTYNPK
                     TKQFYGFPDDYAPDPVQYRRSYLQQAGVSLNTWEDLRKGAPKLKAIGHPVGLGMSNEI
                     DSNMMLTSLLYCYGGFIQNEENRIVIGQGSNRKGAIEALTVMRDIYRNGMSDEVFAWT
                     AASNNQAFLASRLSVALNAISIARSAENTGNTALSDDTFLASIPRGPHTRLGNEHVMG
                     VFVIWKFAKNKEAAKKYLIDQQLGYREHFIRSQYYNFPAWTGAIPGGFKGIRAMTAQD
                     THKPKGKYTILTTIAEKYTTNPGHPGNTTPVMDEIYNTFLIPQMFAEVAQGKSSPGEA
                     VSAFASKAQGIYRKWRNQGLV"
     gene            complement(2630..3493)
                     /locus_tag="VMZ33_07985"
     CDS             complement(2630..3493)
                     /locus_tag="VMZ33_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HUR17211.1"
                     /translation="MNWREKGVTYAVVVPFAILLAFPFYFALVTMFKTNLDLANVEHS
                     PYLYNDLEGGFHWDFWHYATTEHVAFLFRETNYPTWLLNTLLVGGAVVGITLLLALPA
                     GYALARLAGGWGQSAGIAIFLVYLIPPTLLFLPLSRLITQLDLKDSLWSLILIYPSFT
                     VPFATWLLMGFFKTIPQELEDAALIDGASRLKALFLIILPISLPGILTAVIFAFSLCV
                     NEFIYAFTFVSTSEQRTVSAGIPNDLIRGDVFFWQSLMAATLIPAIPLALLYNAFLDR
                     FIKGFTGGAFR"
     gene            complement(3493..>3850)
                     /locus_tag="VMZ33_07990"
     CDS             complement(3493..>3850)
                     /locus_tag="VMZ33_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUR17212.1"
                     /translation="IFIAGRASIPTEVEDAAKMDGATGVKKLWYVDIPLQLPIALVAV
                     LFGIVFTAVDFSVVYILTRGGPFNSTQVLPTWAYFVGIDGGSLGEGAAISLFLFPLLV
                     VVSVAMLFFARRAQVS"
BASE COUNT          652 a         1251 c         1270 g          677 t
ORIGIN      
        1 ataccctccc gccctgcatg ctgcggcccc gcttcggcgc gctctgcgcg ctgctcgtca
       61 gcctgatcct cgtctcgccg gcttccgccg tccctgacgg ccccctcgac gtctatctcg
      121 agcacctcga gccgctgacg ctcgagtggc ccgcgcaggg cacggtcacg gacgggttcg
      181 gcctccgctg gggccggccg cacctcggca tcgacgtcgg catcctcacc tcgctcgacg
      241 tccgtgcggc caggagcggg acggtgaccg aggtcggctg gctgacgggg tacgagggct
      301 acggaaatgt cgtcatggtc gacgtgggca gcggctactc cgtgctctac gcccatctga
      361 gcgcgaccag cgtcgttccg ggccagtggg tggacgacgg cgatccgatc gggactgcgg
      421 gctgcaccgg ctcctgcacg ggcacgcacc tgcacttcga gctgcgcagc ggcggcgttc
      481 cggtcgaccc gtttccctat ttgaacaagt aaacctcccc cgaacgaggg atgcccgcct
      541 cggggagcgg ggctacgttg agcgctgcat atgacacgga cgctccctca gcggagcgcg
      601 ctcgtcgtcc tgctcctctc tctcgtcgtc gcccctgcgg cctcggcacg cacggacagc
      661 gccgcgcgtc tccagatggc ctggcccgcc agcggcaccg tcacctccca ctacggcgag
      721 tggcgcggta gccgccgcca cgacggcatc gacatcggca tgctgcgcac gctgcggctg
      781 cgcgcagtaa cggcgggcgt cgtgcggaag accggctacg tgcccggcta cgctggctac
      841 ggcaacgtcg tcgtcctggc gctgcctggc ggctactcgg cgttgtacgc gcatctctcg
      901 caggtcgggg tcaaacctgg ccagcgcgtc cgcaagggcc agcgcatcgg cctcgccggc
      961 tgcacgggca gctgctccgg cacacatctc cacttcgagg tccatcgcaa gggccgctcg
     1021 gtaaacccgc tgcgcttcct cggctagaat ccagcccgcg ggccgttagc tcagttggga
     1081 gagcaccagc tcgacaagct gggggtcgct ggttcgagcc cagcacggcc cacctcgacc
     1141 gcggtgcaga accgccgaaa gcgacggttt ctgcaccgcg gagattttct agacgagccc
     1201 ctggttcctc cacttccggt agatcccctg cgccttgctc gcgaaggccg agacggcttc
     1261 gccggggctg gacttcccct gcgcgacctc cgcgaacatc tgcgggatca ggaaggtgtt
     1321 gtagatctcg tccatcaccg gcgtcgtgtt gcccggatgg cccgggttcg tggtgtactt
     1381 ctcggcgatc gtcgtgagga tcgtgtactt gcccttaggc ttgtgcgtgt cctgtgccgt
     1441 catcgcgcgg atgcccttga aaccgcccgg gattgcaccc gtccacgccg ggaagttgta
     1501 gtactggctg cggatgaagt gctcgcggta gccgagctgc tggtcgatca ggtacttctt
     1561 cgccgcctcc ttgttcttcg cgaacttcca gatcacgaag acacccatga cgtgctcgtt
     1621 gcccaggcgc gtgtgtgggc cgcgagggat cgaggcgagg aaggtgtcgt ccgagagagc
     1681 cgtgttcccg gtgttctcgg ccgagcgcgc gatcgagatc gcattcagcg ccaccgacag
     1741 ccggctggcg aggaacgcct ggttgttcga ggcggcggtc cacgcgaaga cctcgtcgga
     1801 catgccgttg cgatagatgt cgcgcatgac ggtcagcgcc tcgatcgcac ccttcctgtt
     1861 cgagccttgg ccgatgacga tccggttctc ctcgttctgg atgaatccgc cgtagcagta
     1921 gaggagtgac gtcagcatca tgttggagtc gatctcgttc gacatgccga ggccgacggg
     1981 atggccgatc gccttgagct tcggggctcc cttccggaga tcctcccagg tgttgaggct
     2041 gacccccgct tgctgcaggt agctgcggcg gtactgcacc ggatcgggcg catagtcgtc
     2101 cgggaagccg tagaactgct tcgtcttcgg gttgtaggtg ctcttcctcg cgaccctcga
     2161 catcggcccg agcttcctgc tgacctcctg cacgatgtcg gtgacgggaa cgacctgctt
     2221 ctggaacgtc gacggcggtg agatgaacca gaacaggtcg tgaccgctct gtgccgcgac
     2281 ctgcgatgcc cctgtgctgt agagcagggc gttgttgatg tggtcgatct tgacctcgac
     2341 gtcgttcgcc tcgccccagc gcttgacgta ggtgttgtcg agccactggt cgtaggcggg
     2401 gacgaagtgg gcccactgca ggatcctgag ctgtcccgag agctgccggt tcgcgtactt
     2461 gagcgggccg tagaacccga gcgctcggtc ggtcaggccg ccgaacatgc cgatcgtgaa
     2521 ggcggcggcg gttccccgct tcagcaggtc gcctcgggtc agttccgagc tcgggcgaac
     2581 gaccccgtct gctacatctg ccacgattac ctcctttgga cgcgattctc taccggaacg
     2641 cccctcctgt aaagcccttg atgaagcggt cgaggaacgc gttgtagagc agcgcgagcg
     2701 ggatggccgg aatcagcgtc gccgccatga gcgactgcca gaagaagacg tctccccgga
     2761 tcaggtcgtt cgggatgccc gcggacaccg tccgttgctc ggacgtagag acgaacgtga
     2821 acgcgtagat gaactcgttc acgcagagcg agaaggcgaa gatgaccgcc gtcaggatcc
     2881 cagggagcga gatcggaagg atgatcagga agagcgcctt caatcgcgac gccccgtcga
     2941 tcagcgccgc gtcctcgagc tcctgcggaa tcgtcttgaa gaagcccatc aggagccacg
     3001 tcgcaaaggg caccgtgaag gatgggtaga tgaggatcag cgaccagagg gagtccttga
     3061 ggtcgagctg cgtgatcagc cgcgaaagcg ggaggaagag cagcgtcggc ggaatcaagt
     3121 agacgaggaa gattgcgatg cctgcgctct gaccccagcc gcccgccagc cgcgcgagcg
     3181 cgtagcccgc cggcagcgcc aggagcagcg tgatgccgac aaccgctccg ccgacgagca
     3241 gcgtgttcag cagccacgtc ggatagttcg tctccctgaa gaggaacgcg acgtgctcgg
     3301 tcgtcgcgta gtgccagaaa tcccagtgga agccgccctc gagatcgttg tagaggtacg
     3361 gggagtgctc cacgttcgcg aggtcgaggt tcgtcttgaa catcgttacg agcgcgaagt
     3421 agaaggggaa cgcgagcagg atcgcgaacg gcacgaccac cgcgtaggtc acgcccttct
     3481 cgcgccaatt catcagctca cctgcgccct tcgagcgaag aacagcatcg ccacgctgac
     3541 caccacgagc agcgggaaca ggaacagcga gatcgctgcg ccctcgccga gggagccacc
     3601 gtcgatcccg acgaagtagg cccaggtggg caacacctgc gtcgagttga agggcccgcc
     3661 acgggtgagg atgtagacga cggagaagtc gaccgcggtg aagacgattc cgaagaggac
     3721 cgccacgagc gcgatcggga gttgcagcgg gatgtcgaca taccagagct tcttcacacc
     3781 cgtcgcgccg tccatcttgg cggcgtcctc gacctcggtg ggaatcgacg cacggccggc
     3841 gatgaagatc
//
LOCUS       DATLCO010000343         3088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8786338, whole genome shotgun sequence.
ACCESSION   DATLCO010000343 DATLCO010000000
VERSION     DATLCO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8786338"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="thyX"
                     /locus_tag="VMZ33_07995"
     CDS             complement(<1..795)
                     /gene="thyX"
                     /locus_tag="VMZ33_07995"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HUR17213.1"
                     /translation="MAAESVTAPIASANPAAVESRTPRSPATRDLGMKATLLFPTREI
                     AEQYYLPLIYTACRTCYSELMPEDIFDRAKTGQVASDKQQDLIRRVIESGHGSTIEHI
                     VFTFAISGVTRTLSHQLVRHRAGVAFDQQSQRYLNYKRPSYMVPGSLQDAPQEMRDRF
                     TAEMDDSLAFYGEMLEAGIPGEDARFVMPNATRTNLIMTANLRALIHMSGLRLCTMAQ
                     WEIRRLFQLIRHEVFSVSPFLGSFLAPKCVPLGYCDEDRNRDEHCPI"
     gene            complement(826..1365)
                     /locus_tag="VMZ33_08000"
     CDS             complement(826..1365)
                     /locus_tag="VMZ33_08000"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HUR17214.1"
                     /translation="MVPLRSDLIGASMWYEAFIGRNGGFEVNITAGSGDCMAGCINQR
                     TWHYAVDSEGTVTLESEEGDEVELSAPTPSEDPSNVQILLNAGPVCPVEQIPADPDCA
                     ARAVANATVTLYAADGTEVAMATTGANGMVAFEVPAGAYYVVAQQVEGYMGTPDAQAF
                     STVGGDAVGLVMSYDTGIR"
     gene            1442..2233
                     /locus_tag="VMZ33_08005"
     CDS             1442..2233
                     /locus_tag="VMZ33_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUR17215.1"
                     /translation="MGSGRGVGEGSGAGLGDKAGDEPGELDGLAPPLQPIIRAATTAA
                     SNIVIRRFVFMVPRREIFVRGSPTCGILAPMVLRRFLGRDDTTGQGEGPIPAQPRSGS
                     GGETETVRRIVAELEQMDPEQRRFLAGFAYILARAAHSDLTVGDDEQSLMEQIVTDIG
                     GLTESQAVLVVQIAKHQTELYGGTEDFLVTREFKGRATDEQKRKVVESCFAVVAADHQ
                     ITAQEYAEMTQIADELDLTREDLNAIRNQYRDHLVAIQRMRGLTT"
     gene            complement(2272..2967)
                     /locus_tag="VMZ33_08010"
     CDS             complement(2272..2967)
                     /locus_tag="VMZ33_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HUR17216.1"
                     /translation="MLSRWNTVIAEIVGTFLFFFVGIGAAVQVGGDAAGLIVVALAHG
                     VALAVLVSSLGPVSGAHFNPAVTIAVWIAGQIPGRRALAYILAQLIGGLAAALAVKYL
                     LPGAAAGTVGRPALGAGIDPVAAIAIEAILTAVLLIAVFGTAIDPRAPKLGGLMIGFA
                     VAADILMGGPLTGAAMNPARWFGPAAVTGVWDNSFVWIIGPLLGAAAAALAYRYVFLP
                     EADLLRTPAEPTA"
BASE COUNT          559 a          968 c         1069 g          492 t
ORIGIN      
        1 gatgggacag tgttcgtcgc ggtttcgatc ttcgtcgcag tagcccagtg gcacgcactt
       61 tggtgccagg aacgatccca ggaacggcga tacggagaac acctcgtggc ggatgagctg
      121 gaacagccgc cggatctccc attgggccat ggtgcacagg cggagaccgc tcatgtggat
      181 cagcgcccgt aggttcgcgg tcatgatcag gttggtccgc gtggcgttgg gcatcacgaa
      241 ccgcgcgtcc tcgccgggaa tgcccgcctc gagcatctcc ccgtaaaacg cgagggagtc
      301 gtccatctca gccgtgaacc ggtcacgcat ctcctgtggc gcgtcctgga gggagccggg
      361 caccatgtac gacggccgct tgtagttgag gtagcgctgg gactgctggt cgaacgccac
      421 gccggcgcgg tggcggacga gctggtgcga cagggtgcgc gttacgcccg agattgcgaa
      481 tgtgaagacg atgtgctcga tcgtggaccc gtggcccgac tcgatgacgc ggcggatcag
      541 gtcctgctgc ttgtcggagg ccacctgtcc ggtctttgcc cggtcaaaga tgtcctccgg
      601 catgagctcg gaatagcagg tccggcaggc ggtgtagatc agcgggaggt agtactgctc
      661 ggcaatctca cgagtcggaa aaagcagcgt cgccttcatc cccaggtcac gagtggcggg
      721 cgaccgcggg gtgcgtgatt cgaccgcagc agggttcgcc gaggcgatgg gggcggtgac
      781 ggattcagca gccatttggc tgcctagatt acgcgtcggc cggggttagc gaatgccggt
      841 gtcgtagctc atgacgagcc cgactgcgtc gccgccgacc gtgctgaagg cctgggcgtc
      901 gggcgttccc atgtagcctt cgacttgctg cgcaacgacg tagtaagcac cggcaggcac
      961 ctcgaacgcg accatgccgt tagcgcctgt ggtcgccatg gcgacctccg tgccgtctgc
     1021 tgcgtacagc gtcaccgtgg cattggcgac tgcgcgggcc gcgcaatcag ggtcggccgg
     1081 aatctgctcg acggggcaca caggtccggc gttgagcagg atctgaacgt tggatggatc
     1141 ttcggacggt gtcggcgcgg acaactccac ctcatcgcct tcttcacttt caagcgtcac
     1201 ggtgccctcg gaatcgaccg cgtagtgcca ggtgcgctga ttgatgcaac cagccatgca
     1261 gtcgccggag ccagcggtga tgttgacctc gaagccgccg ttgcggccga tgaatgcctc
     1321 gtaccacatg gatgcgccga tcaaatcgga gcggagcgga accatggttg cccatcgggg
     1381 atcggatgcg aacacgagag cggctgcctg agccggcgtc gagacgacgg ttggccccgg
     1441 cgtgggatca gggcgcggtg tcggcgaggg gagtggggcc gggcttggcg acaaagccgg
     1501 cgatgaaccc ggcgagctgg acggccttgc gccgccgctg caaccgatca tcagggcggc
     1561 gacaaccgct gcgagcaaca tcgtgattcg tcggtttgtg ttcatggtcc ctcgacgcga
     1621 gatattcgtt cgtggttccc ccacgtgtgg catcctcgca cccatggtgc tcagacgatt
     1681 cctcggcagg gacgacacga cgggccaagg cgaaggcccg attcccgctc agccacgatc
     1741 cggcagcggc ggtgaaaccg agaccgtccg tcgcatcgtt gccgagctcg agcagatgga
     1801 tccggagcag cggcggttcc tggccgggtt cgcgtacatc cttgcgcgag ccgcgcacag
     1861 cgacctgact gtcggcgacg acgagcagtc gctcatggag cagatcgtga ccgacatcgg
     1921 cgggctgacc gagtcgcagg ccgtgctcgt cgtacagatc gccaagcacc agacggagct
     1981 ctacggaggg acggaggact tcctcgtgac gcgcgagttc aagggccggg caaccgacga
     2041 gcagaagcgc aaagtcgtcg agtcgtgctt cgccgtcgtc gctgccgacc atcagatcac
     2101 ggcccaggaa tacgccgaaa tgacccagat cgctgacgag ctggacctaa cgcgcgaaga
     2161 cctgaacgcg atacgtaatc agtaccgcga tcacctcgtg gcgatccagc gaatgcgggg
     2221 cctcaccacc tagggatcgc gccgacctaa cccttggagt cgggtcaagc ctcaggccgt
     2281 tggttcggcc ggtgtcctca acaggtctgc ctcgggcagg aagacatagc gataggccag
     2341 ggcggcagcg gcggcgccga gcagcgggcc gatgatccag acgaagctgt tgtcccagac
     2401 gccggtcacg gccgcggggc cgaaccagcg ggcggggttc atggccgcgc ccgtgagcgg
     2461 gccgcccatc aggatgtcgg ccgcgaccgc gaaaccgatc atgaggccac ccaatttggg
     2521 cgcgcgcggg tcgatggctg tgccgaagac cgcgattagc agcacggcgg tcaggatcgc
     2581 ctcgatggcg atcgcagcga cggggtcgat gccggctcca agggctggac ggccgaccgt
     2641 gcccgcggcc gccccgggca gcaggtactt gacggccagg gccgcggcca agccaccgat
     2701 gagctgcgca aggatgtacg cgagtgcgcg acggccgggg atctgaccgg caatccaaac
     2761 cgcgatggtg acggctggat tgaagtgcgc gcccgatacc ggcccgagcg agctgaccag
     2821 cacggccagc gcgacgccgt gcgcgagcgc gacgacgatc aggccggcgg cgtcaccgcc
     2881 aacctgcacc gcggcgccga tgccgacgaa gaagaacagg aacgtaccga cgatttcggc
     2941 gatgaccgtg ttccaccgac tgagcatccg gcctctctcc ctctgttcgc aacgcggggc
     3001 ttgccccgca tcgccatact acgcaagccc atacgtcacg ggagggaaga ttggcactga
     3061 cgcacccact gtcgggtgac gccgcgcg
//
LOCUS       DATLCO010000344         2471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3594476, whole genome shotgun sequence.
ACCESSION   DATLCO010000344 DATLCO010000000
VERSION     DATLCO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3594476"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1432
                     /locus_tag="VMZ33_08015"
     CDS             248..1432
                     /locus_tag="VMZ33_08015"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HUR17217.1"
                     /translation="MRAADLSAQEALVYLDQARTLIAESADLYEAGLNDEAYTAARNA
                     YLDNFEFVEISLRVRDDALTLELEEDFARLRGAMQQGEPASEVTAIAAEVLNGLDRVE
                     RTLSEPGLAAPFIAAVSAFTILFREGLEATLVVAAVLGYLEASRNQQYRGAVLRGVLG
                     ALIATAVIFVIVSTLVRLAPVQREIIEAGTALIASGVLFYVSFWLVSKIDQRRWMEFV
                     KAKVFAAAATGSAVALFFVGFTSVFREGLETVLFYQALLAFGEGLELWIALGTAVAVA
                     VLAVLAYVILVAGRRVPVKRFLQVAVILIMALSVAFIGNAVRALQEIELVPISFIESA
                     PRLPIFLTDLLGWHPTVQTIAAQLILAAIYVVGGLYVAVIGPRRGAKHANGARPGPAP
                     AT"
     gene            1458..>2471
                     /locus_tag="VMZ33_08020"
     CDS             1458..>2471
                     /locus_tag="VMZ33_08020"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HUR17218.1"
                     /translation="MDVGGTFTKAVAVAPDGTIVARSIVPTTHSARGGVAVGVAEALE
                     SVAGTLTDAGGGRIALVTHSTTQAVNALLEGDTATVGVLAIGHRPDLDRTRRRTALGP
                     IGLAPGRSLRTVSTLIDATDGLADEVVDRALDELVARGAEAIVVSAAFGAEDPTMERA
                     VLEACVARGIAACAGHELSGLYGLELRTVTGAINASILPISVEAARVVGEAVVRVAPN
                     AGLLVMRGDGGAADLEMMRRRPLMTAFSGPAASVSGALRRLELVDAICVEVGGTSTNV
                     AIVKGGRAVLSYVRVLEHQTSVRSLDVRVVGVAGGSLMRVSRRLGRLRVSDVGPRSAH
                     IAGL"
BASE COUNT          358 a          823 c          826 g          464 t
ORIGIN      
        1 ctgacatcaa cgacgagctc gagccgcttc tgggggccgg actccgtcag cagatcaacg
       61 cgggcgcgac ggtcgaggag atcgatggga ccatcgctcg cgtcaaggct ctgctcggcc
      121 aggccatcga agctctcgag cactgatgtc atcggcggga ggcttcgcac gcccgaccgt
      181 gcgagcttcg attctgggcg gactctttct cctgattgcc ttcgccggac ttttcaatgc
      241 agctccggtg agggccgctg atctgtcagc ccaggaagcg ctggtctacc tcgaccaggc
      301 acgcacgttg attgcggaga gcgccgacct gtacgaggcc ggcctcaacg acgaggccta
      361 cacagcagcc cgcaatgcat atctcgataa cttcgagttc gtcgagatct cacttcgggt
      421 gcgtgacgat gccctgaccc tcgagctcga agaagacttc gcgcgactgc gcggggccat
      481 gcaacagggt gaaccagcga gcgaagtgac ggcaatcgca gccgaggtcc tgaacggtct
      541 ggaccgcgtc gagcggaccc tgtcagaacc cggactcgct gcgcctttca tcgccgcagt
      601 ttcggcattc acgatcctgt tccgcgaagg gcttgaggcc accctcgtcg tggcagccgt
      661 gctgggctat ctggaggcaa gccgcaacca gcaataccgc ggcgccgtcc tgcgcggtgt
      721 gctgggcgcg ctgatcgcca cggccgtgat cttcgtcatc gtcagcacgc tggtgcgcct
      781 ggccccggtc cagcgcgaga tcatcgaagc cggcaccgcc ctgatcgcct ccggcgtcct
      841 cttctacgtt tcgttctggc tggtgtcgaa gattgaccag cgccgctgga tggagttcgt
      901 caaggcgaag gtctttgctg ccgcagcaac cggttcggcc gtcgcgctct tcttcgtcgg
      961 attcacatcg gtgttccgcg agggcctcga gacagtcctt ttctaccagg cactgctggc
     1021 cttcggcgaa ggactggagc tctggatcgc tctcggcaca gccgttgcgg tggccgtgct
     1081 cgcggtgctc gcctatgtga tcctcgttgc cggccgccgc gttccggtga agcggtttct
     1141 ccaggtcgcg gtgatcctga tcatggcgct gtcggtcgcc ttcatcggca acgccgtccg
     1201 cgcactccag gagatcgagc tcgtgcccat ctcattcatt gaatccgctc ctcgactgcc
     1261 gatcttcctg acggacctcc tgggctggca cccaacggtc cagaccatcg ccgcccagct
     1321 catccttgct gccatctacg tcgtcggcgg tctgtacgtc gccgttatcg ggccgcgccg
     1381 cggggcaaag catgccaacg gcgcaagacc gggacccgcg cctgccacct gatccggccc
     1441 gggcggttag ggttggcgtg gacgtgggtg ggacgttcac gaaggccgta gccgtagcgc
     1501 cggacggcac catcgttgcc cgctcgatcg tgcctaccac ccactcggcc cgtggcggcg
     1561 tcgccgtggg tgtggctgaa gctctcgagt cggtggccgg cacgctcacg gacgccggcg
     1621 gcggccgaat tgcgctggtg acccattcca cgacccaggc ggtcaacgcg ctgctggaag
     1681 gcgacacggc cacggtcggt gtcctggcga tcggtcatcg gcccgacctt gaccgaaccc
     1741 gccgacgcac ggcgctcggg ccgatcggcc tggcacccgg gcgctcgttg cgaacggtga
     1801 gcacgctcat cgacgcaacg gacgggcttg ccgacgaagt tgtggatcgc gccctcgacg
     1861 agctcgtggc acgcggcgcc gaggcgatcg tcgtttccgc cgctttcggc gccgaggatc
     1921 caaccatgga gcgggctgtg ctcgaagcgt gcgtcgcccg cggcatcgcg gcgtgcgccg
     1981 gtcacgaact ctcgggcttg tacgggctcg agctgcgaac ggtcaccggc gcgatcaacg
     2041 ccagcatcct ccccatctct gtcgaggcgg cgcgagtcgt gggtgaggca gttgtgcgag
     2101 ttgcgcccaa cgccggtctc ctcgtgatgc gcggcgatgg cggtgcggct gatctcgaga
     2161 tgatgcgccg tcgccccctg atgaccgcgt tcagtggccc ggcagcgtcg gtgtcgggcg
     2221 cgttgcgccg gcttgagctt gtggacgcga tctgcgtgga ggttggcggc accagcacca
     2281 acgtggccat cgtgaaaggc ggccgggccg ttctttcgta tgtacgcgtc ctggagcatc
     2341 agaccagcgt tcgcagcctg gacgtccgcg tggtcggtgt cgccggtggg agcctgatgc
     2401 gcgtttcgcg tcggttgggg cgcctgcgcg tgtccgatgt cggaccgcga tcggcgcata
     2461 tcgcgggcct c
//
LOCUS       DATLCO010000345         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_11042033, whole genome shotgun sequence.
ACCESSION   DATLCO010000345 DATLCO010000000
VERSION     DATLCO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11042033"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VMZ33_08025"
     CDS             <1..1173
                     /locus_tag="VMZ33_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUR17219.1"
                     /translation="GAGFGRMGSRALVVVAEVALAVVLCVGAGLLVRSFVRLVNVNPG
                     YDARDVTTFQIVPPTAHAGDPTRLYEEVLSRLDADPSIQAIAATDVLPIAGASAFHMS
                     LGGLPLEPGSEPMIMRIVTAGYFDAMKMRMIAGRSFSGGGRPAYPELIVNQEFVRRYL
                     SGAEPIGQLVGNPTRYQVVGVVNNVRHGSLSAGVRAEYYVDLSRFGLTEAVRPYFVVR
                     SAADRGDVASLIRSAVRSVDPQLGVDLNQQSMAELVSESVAKPRFNTFVLGAFAVVAL
                     ALATVGIYGVISHAVTQRTREIGIRMAIGATPSRVLAAVLRQSLTLTGVGAAIGSLGA
                     AAASRYLESMLFELTPLDISTFVGVAVLFLVVALLAAYIPAARASRVDPLVALRHL"
     gene            complement(1431..>2070)
                     /locus_tag="VMZ33_08030"
     CDS             complement(1431..>2070)
                     /locus_tag="VMZ33_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HUR17220.1"
                     /translation="VARMYAQPPDPGFDMTVSYVRKLPREAPPGTKWVYKTSETNLVG
                     VLVAEATGKHLADYLAEKIWRPYGMERDAEWMIDDVGHEQGGCCLGMTLRDYGRFGQF
                     IVDGAGVNGRSIVQENWLAEATRTQVSTGSGTGTGYGYQWWTREDGTFEGRGIYGQTL
                     HIDRGRRLVIVINSATEAPTGRGAGQARQDFIAAVNAELDAPAPAPSLRRQD"
BASE COUNT          302 a          695 c          669 g          404 t
ORIGIN      
        1 ggggcaggct tcggaaggat ggggtctcgc gcactcgtcg tcgttgcgga ggtcgcgctg
       61 gctgtggtgc tctgtgtcgg tgcgggcctg ctcgtccgca gcttcgtgcg gctggtgaac
      121 gtcaatccgg gatacgacgc gagggacgtc acgacgtttc agatcgtgcc gccgacggcg
      181 cacgccggcg atccgacgcg actctatgag gaggttctgt ccagacttga tgccgatcca
      241 tccatccagg ctatcgccgc aaccgacgtg ctgccgatcg caggggcgag cgcatttcac
      301 atgagccttg gaggtttgcc gctcgaacca ggatcggaac cgatgattat gcgcattgtc
      361 accgccgggt atttcgacgc catgaagatg cgaatgattg caggccggtc gttctccgga
      421 ggcggccggc ccgcttatcc cgagctgatc gtcaaccagg agttcgtccg ccgctatctc
      481 tctggtgcgg agccgatagg tcagctcgtc ggtaacccga cgcggtatca agtggtggga
      541 gtggtcaaca acgtcagaca cggcagcctc tcggccggtg ttcgagccga gtactacgtc
      601 gacctgagcc gctttggtct aaccgaggcg gtgcggccgt atttcgtcgt ccgaagcgca
      661 gcggatcgcg gcgacgtggc gtcgctgatt cgctctgcgg tgcgcagtgt ggatcctcag
      721 ttgggcgtgg acctcaatca gcaatccatg gcggaactgg tgtcagagtc ggtggccaaa
      781 ccacggttca atacgtttgt cctgggagcc tttgcggtcg tggcgcttgc gctggcgacg
      841 gtcggcatct acggcgtcat atcgcatgcg gtaacccaac ggacgcggga gataggaatc
      901 cgtatggcga tcggggcgac cccctcacga gtgctcgccg ctgtgctccg ccaaagtctg
      961 acgttgacgg gcgtgggcgc agcgatcggg tctctcggcg ccgctgccgc cagcaggtat
     1021 ctggagagca tgctcttcga gcttactccc ttggatatat cgacgttcgt cggcgtcgcc
     1081 gtgctcttcc tcgtggtggc gctgttggcc gcgtacatcc ctgcggcacg ggcaagtcgc
     1141 gtcgatccgc tcgtcgcgct tcgacatctt tgattactcc gaggggcttc gcccctcgga
     1201 cgcccctgca cgcgccctcg ctcgccgctc cgtcggctcc ctccggccgc gtgggctcac
     1261 gcgcacgcgc gttcgccgac cgtggcactg ggagtactcc gaggggcttc gcccctcgaa
     1321 cgcccctgca cgcgccctcg ttcggccagc cttggcgctc gcttctgcac cggctccagc
     1381 ggcaagccct gaacgccgca agccgcgagt cgctgactaa tcgaccgccg tcaatcctgt
     1441 cgccgcaggg agggcgccgg agcgggagcg tccaattccg cgttgaccgc ggcgatgaag
     1501 tcctgccgcg cctgccctgc cccccgccct gtcggcgcct cggttgcgct gttgatgacg
     1561 atgacgaggc ggcggccgcg atcgatgtgc agcgtctggc cgtagatccc ccggccttcg
     1621 aacgtgccgt cctcgcgcgt ccaccactga tagccgtagc ctgtgcctgt gcctgagcca
     1681 gtcgagacct gggtgcgcgt ggcctcagcg agccagttct cctgcacgat agagcgaccg
     1741 ttgaccccag ccccgtcaac gatgaactga ccgaagcggc cgtagtcgcg cagcgtcatc
     1801 ccgaggcagc agccgccctg ctcgtggccg acgtcgtcga tcatccattc ggcgtcgcgt
     1861 tccataccgt aggggcgcca gattttctcc gcgaggtagt ccgccagatg cttccccgtc
     1921 gcctccgcca ccagcacgcc gaccaggttc gtctctgatg ttttgtagac ccacttcgtc
     1981 cccggcggcg cctcgcgcgg cagcttccgc acgtagctca cggtcatgtc gaagcccgga
     2041 tcgggcggct gcgcgtacat gcgcgccaca
//
LOCUS       DATLCO010000346         3783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6460220, whole genome shotgun sequence.
ACCESSION   DATLCO010000346 DATLCO010000000
VERSION     DATLCO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6460220"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VMZ33_08035"
     CDS             complement(<1..672)
                     /locus_tag="VMZ33_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR17221.1"
                     /translation="MAGKGQKTYDAVVIGGGHNGLTAAAYLAKAGLSVVVLERRHIVG
                     GATVTEELWPGFKISVASYVVSLLRPEVIRELELVRHGLEILPVDGTLTPTGTNKGEY
                     LWRVDDHGTTLRELRRHSKRDAEAYEEYSLLMSEMARFVKPLLAAAAPRLGTFDGRGL
                     ADLAGFARRFAALPEPQQTAFIQLLTMSSADFVSQWFETPALRATLAASGIIGTFLGP
                     RSPGTA"
     gene            791..1579
                     /locus_tag="VMZ33_08040"
     CDS             791..1579
                     /locus_tag="VMZ33_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR17222.1"
                     /translation="MSESAAGPDSSTQAARRALLLVDRPVLSQLIKLTLNHGLFVVRS
                     ASDLAEATTVLRDWQPHIAVVDMDLEDSAELLRLMSTSGTFSASVTPSLGLTRRGDLA
                     TKLKAFDLGVDDIVTVPFSPEELLARAIVVTRRRMGADPTFVPTIELGDMEIDILNRE
                     VRTGNSVVHLTSVEQSLLYLLASHSGRVVTRNEIIDAVWGPDFVSESNIVDRHVRSLR
                     IKLQNDFRKPRFIATVPGEGYRFMPTFSNSGWDSGQGEDTNQPH"
     gene            1708..2079
                     /locus_tag="VMZ33_08045"
     CDS             1708..2079
                     /locus_tag="VMZ33_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17223.1"
                     /translation="MGQMDTYAGSARDSGTGPAREFDRRAAESQRPGIDGADTHAPAT
                     NDAPTNGAPTNGHATHGAAVAQVLRAWRAVERQLAGMADDDPEVAMAQQQIARLRDEY
                     HRRVAEESQQQTNDADGTSRP"
     gene            2126..3319
                     /locus_tag="VMZ33_08050"
     CDS             2126..3319
                     /locus_tag="VMZ33_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HUR17224.1"
                     /translation="MTVGTPFHERTAPLNRKQSWREWSGYLAASAYDHLHIHEYSGIR
                     HAAVLIDVSPLYKYIARGPDATRLIDRVITRDATRIQPGQVIYTPWCDDHGKVIDDGT
                     IARLDDDSYRWTSAEQHLRWFELNARGLDVEIEDVSETTAAIALQGPLARDVLGAATG
                     TDWSDLGYYRRRAGHAGKIQIDVSRTGYTGDLGYELWVKAEQATDLWDRLMEAGRPFA
                     LRPAGMLALDMARLEAGLILIEVDYTSATQALIPSQNYSPFEIGLGRLVNLNKGSDFV
                     GRRALLAEQAGGGPKRRLVGLEIDWPSIEANFQQQGLPIRVPTAAWREQVPVYVGGRQ
                     VGRATSGTWSPTLKKHIALAQVDAAHEAVGSRLSIEQTVEGHRGRATATVAPLPFFDP
                     PRKRF"
     gene            complement(3334..>3783)
                     /locus_tag="VMZ33_08055"
     CDS             complement(3334..>3783)
                     /locus_tag="VMZ33_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR17225.1"
                     /translation="RIPHEHPLMGLRLADRAAIDHAIARVGIAHLRGRDVRELSLGER
                     QLVVLAMTVAQGAQLLLLDEPTVHLDLRHQVEVMQLLADLAQRDGVTVLTVLHDLSLA
                     SHFFPRLLLLDEGRLVADGPAGAVLTPDRVREVYRVDPRFVTVATSG"
BASE COUNT          684 a         1259 c         1244 g          596 t
ORIGIN      
        1 cgccgtcccc ggcgagcgcg ggcccaggaa cgtgccgatg atccccgacg cggccagtgt
       61 cgcgcgtagc gccggcgtct cgaaccactg actgacgaag tcggcactgg acatggtgag
      121 gagctggatg aaggccgtct gttgtggctc cggcagcgcc gcgaatcttc gggcgaagcc
      181 tgcgagatcc gccagcccgc ggccgtcgaa cgtgcccagt cgcggcgctg ccgccgcgag
      241 aagcggcttc acgaagcgcg ccatctcgct catgagcagc gagtactcct cgtatgcctc
      301 ggcgtcgcgc ttgctgtgcc gccgcagctc gcgcagggtc gtgccgtggt cgtcgacgcg
      361 ccagaggtac tcgcctttat ttgttccggt cggggtcagc gtcccgtcga cggggagtat
      421 ctcgaggccg tgccgcacca gctcgagctc gcggatgacc tccggccgca acaggcttac
      481 aacgtaagac gcgacgctga tcttgaatcc gggccacagc tcctcagtga cagtcgcccc
      541 gccgacgatg tgccggcgct caaggacgac gaccgacaga ccagctttgg ccagatatgc
      601 cgcggcggtg aggccgttgt ggccgccgcc gatgacgacg gcgtcgtaag tcttctgccc
      661 tttacccgcc acgccgaaac tctatcggcc cgctgccgcc gttcccgttg tgaccaccga
      721 gcgatggaat gacacggtgg gagtaagatc tgcgcgaggg gtgagggtga aaactaaagg
      781 caacctatct ttgagcgaat ccgcggcagg tcccgattct tcgacacagg ccgcgcgtcg
      841 ggcgctgctg ctcgtagacc gcccggtcct cagccagctg atcaagctga ccctcaatca
      901 tggcttgttc gtggtgcgct cagcgtcaga cctggctgag gcaacaacgg tcctgcgtga
      961 ctggcagccg cacattgccg tagtggatat ggatctcgag gacagcgccg aacttttaag
     1021 gctgatgtcc acttccggga cctttagcgc gagcgtcact ccctccttgg gcctgacgcg
     1081 ccgcggcgac ctggcaacca agctcaaggc gttcgacctg ggcgtcgacg acatcgtcac
     1141 cgtgcccttc tcgcccgagg agctgcttgc ccgggcaatc gtcgtcacac ggcgcaggat
     1201 gggcgcggac ccgacattcg tgccaaccat cgagctgggc gacatggaga tcgacattct
     1261 caaccgcgag gttcggacgg gcaactcggt ggtgcacctg acgagcgtgg agcagagcct
     1321 tctgtacctg ctagccagcc acagcggacg tgtcgttacg aggaacgaga tcatcgacgc
     1381 cgtgtggggg cccgacttcg tgtccgagag caacatcgtc gaccggcacg tccgcagcct
     1441 gcggatcaag ctccagaacg attttcgaaa gccccgattc attgcgacgg tgccagggga
     1501 gggctatcga ttcatgccca ccttctccaa cagcggatgg gattccggcc agggcgagga
     1561 tacgaaccag cctcattgat ccgcccttcc cgagggggca cgtcggcgga ttacggaccg
     1621 taatgacatt gcaatcgcgg ccccacaaca tccacccctg agaagaggcg ggcgtgatgc
     1681 gcccgggcag cgttttgggg tggcgtcatg gggcagatgg atacttacgc cggttcggcc
     1741 agggattcgg gtactggccc agcgagggag ttcgaccgtc gagcggcgga aagtcagcgc
     1801 cccggaattg atggcgccga cactcatgcc cccgcgacca acgacgcacc caccaacggc
     1861 gcgccgacca acggtcacgc gacccatggc gcagctgtcg cccaggtgct gcgtgcgtgg
     1921 cgggccgtcg agcggcagct ggccggaatg gctgacgacg accccgaagt agcaatggca
     1981 cagcaacaga tcgcccgcct tcgcgatgag taccaccggc gtgtcgcaga agagtcgcag
     2041 caacagacca acgacgcgga cggaacctcc aggccctaac cacgcccccc tggctcttcg
     2101 ctctggctct cttaggattg cctgcgtgac cgtcgggact cccttccacg aacgaaccgc
     2161 gccgctcaat cgcaagcaga gctggcggga gtggtccggg tatctcgccg ccagcgccta
     2221 cgaccacctg cacatccacg agtactcggg gatccgccac gcggcagtgc tcatcgacgt
     2281 gtcgccgctc tacaagtaca tcgcgcgcgg cccggatgcg acgcgactca tcgaccgcgt
     2341 catcacccgc gacgcaacca ggatccagcc ggggcaggtc atctacacgc cctggtgcga
     2401 cgaccacggc aaggtcatcg acgacggcac aattgcgcgg ctagacgatg actcataccg
     2461 ctggaccagt gctgagcagc atctgcgttg gttcgagctg aacgcacgcg gcctcgacgt
     2521 agagatcgag gacgtcagcg agaccaccgc agcgatcgcc ctgcaaggcc cgctggcgcg
     2581 cgatgtcctc ggcgccgcca caggcacaga ctggtctgac ctcggctact accggcgtcg
     2641 tgccgggcac gctggcaaga ttcagatcga cgtcagccgc acgggctata cgggcgacct
     2701 cggctacgag ctgtgggtaa aggcggagca ggccacggat ctgtgggacc ggctgatgga
     2761 ggcgggtcgg cccttcgcgt tgcgcccggc gggcatgctg gccctggaca tggcccgact
     2821 cgaggccggc ctgatcctga tcgaggtcga ctacaccagc gccacgcagg cactaatccc
     2881 aagccagaat tattcaccgt tcgaaattgg gctgggccga ctcgtcaacc tcaacaaggg
     2941 gtccgatttc gtcggtcggc gggcgctgct cgcagaacag gccgggggcg gtcccaagcg
     3001 tcggctggtc gggctcgaga tcgattggcc ttcgatcgag gcgaacttcc aacaacaggg
     3061 cctgccgatc cgcgtcccca cggccgcctg gcgcgagcag gtgccggtct acgtcggcgg
     3121 ccgacaggtt ggacgggcga ccagtggcac atggagtccg acgctcaaga agcacatcgc
     3181 gctggcacag gtcgacgccg cgcacgaggc cgtcggctcg cggctgtcta tcgagcagac
     3241 tgtggagggc caccgcggcc gcgccacggc aacggtggca ccactgccct ttttcgatcc
     3301 gccacgcaag cgtttctgag cgcggccgct agctcaacct gacgtggcga cggtcacgaa
     3361 tcgggggtcg acgcggtaga cctcacgcac tcggtccggc gtgaggacgg ccccggccgg
     3421 accgtcggcc acgagccggc cttcgtccag caacagcaat cgcgggaaga agtggctggc
     3481 cagcgagagg tcgtggagca cggtcagaac tgttactccg tcgcgctgcg caaggtccgc
     3541 gagaagctgc atgacctcga cctggtgtcg cagatccaga tggacggtcg gctcgtcgag
     3601 cagcagcagc tgcgcaccct gggcgacggt catggccagc accacgagct gacgctcccc
     3661 gagagacagc tcgcgcacgt cgcggccgcg caggtgcgcg atgccgacgc gcgcgatggc
     3721 gtggtcgatg gccgcgcggt ccgcgagtcg cagacccatc agcgggtgct cgtgcgggat
     3781 gcg
//
LOCUS       DATLCO010000347         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7354214, whole genome shotgun sequence.
ACCESSION   DATLCO010000347 DATLCO010000000
VERSION     DATLCO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7354214"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..95
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            248..1243
                     /locus_tag="VMZ33_08060"
     CDS             248..1243
                     /locus_tag="VMZ33_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HUR17226.1"
                     /translation="MSRPTRVFLLSLALITAACSGAPPPSATLHVDPTIPTAFPLALR
                     DDEGAEVTITAEPQTIVSLTPANTEIVYALGAGDRMRGGTDFDDFPAEAAALPDVVAG
                     VQVLTEQIVAIDPDLVLAGGNNFTPAAEVERLRGLGITVLVVYPETVDEVVADIRLIG
                     RAIGRHDEAMAITEGMEARIRDITSVVSRLEPLRTFYEIGYGPDIYGPAPNSFVADMV
                     ELAGGEAITSSDPAVFSISLEDLVDKDPEVIVLGDAAYGTCPETVPSRPGWDSMTAVA
                     DAAIRPVDDIVVTRPGPRLAEGLAALALAIHPDAQIKPPTAGAVLCADPGVSSTP"
     gene            1240..>2084
                     /locus_tag="VMZ33_08065"
     CDS             1240..>2084
                     /locus_tag="VMZ33_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HUR17227.1"
                     /translation="MNAEATARRAAARPAWLGFLRHRPVALVVLGVGALCATLITGVW
                     LGSVGIAPGDVLAILTHRLFRWPADQTWPLSAETIVTELRLPRVLTAMAVGAALSVAG
                     ATYQGLLRNPLADPYVLGTASGAALGAAIAVLIPIQIGLLGLGMLHLLAFVGALLAVA
                     VVYRVSRLGALNSLTSVLLTGYAIGSLLAAGLAMAMYLSGNNLRQIFYYLLGSFSLAS
                     WPQLAVSALLIAIGLGLVLSRSRSLNALLLGEEAAAHLGIDVRRDRLILLGGATLMTA
                     AAVAV"
BASE COUNT          301 a          744 c          683 g          356 t
ORIGIN      
        1 gtgtgaatcc ggcacagtcc cgctacggtg accggcgctc aacctctggg tgagtgccgg
       61 aagtccggtc gccgggccca tggtcgtgcc gtatttcctt cgtgagaaag ggaacgcacc
      121 catgagccat gaccagaaaa gcgccccttc cgcttcgcgc ggcaaggggt tttctcatgc
      181 ccgcaagggc acgccgggcg cctccaccag gcatacgacc cccgcgcgac ctgcgcagag
      241 agacacgatg agccgcccca cccgagtttt tctgctgagc ctcgccctga tcaccgccgc
      301 atgctcgggc gcaccacccc cgagcgcgac actccatgtc gacccgacga tcccgacggc
      361 cttccccctg gctctgcgcg acgacgaggg cgcggaggtc accatcacgg ccgagccgca
      421 gaccatcgtc agcctcacgc cggcgaatac tgaaatcgtc tacgcccttg gggccggcga
      481 ccggatgcgc ggcggcacgg actttgacga cttccctgcc gaggccgcgg cattgcccga
      541 cgtggtggcg ggcgtccagg tgctgaccga gcagatcgtt gcgatcgatc ccgacctcgt
      601 tctcgccggc ggcaacaact tcacgcccgc cgcggaggtc gaacggctgc gcggcctggg
      661 catcaccgtg ctcgtggtct accccgagac cgtcgacgag gtcgtggccg acatcaggct
      721 catcggccgc gcgattggac gacatgacga ggctatggcg atcacagaag gaatggaggc
      781 ccgcattcgc gacatcacca gcgtggtctc gcgtctggag ccgctgcgca cgttctacga
      841 gatcggctac gggccggata tctacgggcc tgccccaaac tcgttcgtcg cggacatggt
      901 cgagctggcc ggtggtgagg cgatcacgag cagtgatccg gctgtgttca gcatttcgct
      961 cgaagatctt gtggacaagg atcccgaggt catcgtcctc ggcgacgctg cctatgggac
     1021 gtgccccgag accgtgccca gccgtcccgg gtgggactcg atgacagcgg tcgccgacgc
     1081 tgcgatccgg ccggtagacg acatcgttgt gaccaggccg gggccgcgac tggccgaagg
     1141 gctggcagcc cttgcgctgg caatccaccc cgacgcccag atcaagccac caaccgcggg
     1201 cgcggtgctg tgcgcggacc cgggggtctc gtccacaccg tgaacgcgga ggcaaccgcc
     1261 cgacgagcgg cggcgcgccc tgcctggctc ggattcctgc gccatcgacc ggtggcgctg
     1321 gtcgtgctcg gtgtgggcgc cctgtgcgcg acgctcatca ccggcgtgtg gttggggagc
     1381 gtcggcatcg ctcccggaga cgtgctggcc atcctgacgc accgcctctt ccgctggccg
     1441 gccgaccaga cctggccgtt gagcgccgag acgatcgtca ccgaattgcg actgccgcgc
     1501 gtgctcacgg cgatggccgt cggagcggct ctgtccgtcg ccggtgccac ctaccagggg
     1561 ctgttgcgca accccctcgc cgacccctac gtcctgggca cggccagcgg cgccgcactg
     1621 ggtgccgcca tcgccgtcct gatcccgatc cagatcggcc tgctcggact cggcatgctc
     1681 catctcctcg cgttcgtcgg cgcgctgctg gccgtggcgg tcgtctatcg ggtctcccgg
     1741 ctcggagcgc tcaactcgct cacgtccgtg ctgttgaccg gctacgccat cgggtcgttg
     1801 ctggccgcag ggctggccat ggcgatgtac ctctcgggca acaacctccg ccagatcttc
     1861 tactacctgc tgggttcctt ctcgctcgcg tcgtggccac agctggccgt atcggccctg
     1921 ctgatcgcca tcgggctcgg gctcgtcctg tcgcgctcgc ggtccctgaa cgccttactg
     1981 ctgggcgagg aagcggcggc tcacctgggt atcgacgttc gccgtgatcg cctgatcctc
     2041 ctgggcggcg cgaccctgat gacggccgcc gcggttgcgg taag
//
LOCUS       DATLCO010000348         3005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10201522, whole genome shotgun sequence.
ACCESSION   DATLCO010000348 DATLCO010000000
VERSION     DATLCO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10201522"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="VMZ33_08070"
     CDS             <1..2154
                     /locus_tag="VMZ33_08070"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HUR17228.1"
                     /translation="LEVIGTPTKGTLWSVRYHLVDDAGQPLPDATITVATTRPETILG
                     DTAVAVHPNDARYTALVGKRVLIPFVDRVVPIIADDVVRQDFGTGAVKITPAHDNDDF
                     ETGKRHGLPLIDVMTDNGHINDSGGPYRGQATEEARKQILADLDAHGDLVGSIEHDMV
                     LGRCERSGHVIEPRVKTQWFINVKPMAARAMAAVDEGRTTFVPPRYRKVFFDWMENIH
                     DWNVSRQLWWGHRIPAWYCPDGHITVSELLEGPQVCADCVRPQSELRQEDDIFDTWFS
                     SGLWPFSTLGWPDDTADLRTYYPTTVMETGYDILFFWVARMMMLGEWLTDRAPFATVY
                     LHGIVRDPYGQKMSKSKGNVLDPLAVIEELGADALRFSLLHGPEPSQDQRMARPRLEE
                     SRNFANKIWNAARFVHSVRPPDVGSDAKLGLVEIDQLGPAEHWILDRCAGTVEAVDRA
                     YGTYEFGEVARLLYSAVWSDFCDWYLELAKIGLNDESASPARKRAIWSTLTWALDRYL
                     RLLHPVMPMLTEEIWQTLPHTADDPELLIVAPWPRAEDTPMKAQSRLADGTDQLIDLI
                     TQIRAARAESGIAAADWLDARIWLPDGAAHETYSSLEAALSRLARIRPQLAANRDELD
                     GAGAQNSLAIITPTVEARLMRSDADRKRERVRLERELAAADAALRSSDARLADPNFTS
                     RAPETVVEQARARADELRNHADALRARLDGTGEGA"
     gene            2154..2891
                     /locus_tag="VMZ33_08075"
     CDS             2154..2891
                     /locus_tag="VMZ33_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17229.1"
                     /translation="MPSIPFIGRKKKPARGGPPRPQLAPDQLELNDVQLRLQYRAVSS
                     HGVRMPSVHDNPVRLPSLLEGFATTPVELIEPLPLEFQDAAPSIVRPESALHWINVHH
                     GRTPIGRHALMILESLDSLDLAYETLAVALLYGDVDGEGFPRFDAIVGGPVSYWDENS
                     GDLVVRLVLAWGGDGARGDTHRVATRLMARLLVNLLASQGATELGRVAKPVAVTGGSQ
                     IRCSHCGFDALDRRAHYCPKCGMRTGA"
BASE COUNT          523 a          997 c          975 g          510 t
ORIGIN      
        1 ctggaggtca taggcacgcc gacgaagggc acgctgtggt cggtccgcta ccacctcgtc
       61 gacgacgcag gtcagccgct ccccgacgcc accatcaccg tggccaccac tcgccctgag
      121 acgatcctcg gcgacacggc cgtggccgtg catccgaatg acgcccgcta cacggcgctt
      181 gtcggcaagc gagtgctcat cccgttcgtg gaccgcgttg tcccgatcat cgccgacgac
      241 gtggtgcgcc aggacttcgg caccggcgcg gtcaagatca cgccggctca cgacaacgac
      301 gacttcgaga cgggcaagcg tcacggcctg ccactgatcg acgtgatgac cgacaacggc
      361 cacatcaacg acagcggcgg cccttatcga ggtcaagcga cggaggaggc ccgcaagcag
      421 atcctggccg acctcgatgc gcacggcgat ctcgttggtt cgatcgagca cgacatggtc
      481 ctcggccgct gcgagcggag tggccacgtc atcgagccgc gcgtcaagac gcagtggttc
      541 atcaacgtga agcccatggc cgcgcgggcg atggcggcag ttgacgaggg ccgaacgacg
      601 ttcgtgccgc cgcgttatcg caaggtcttc ttcgactgga tggagaacat ccacgactgg
      661 aacgtcagcc ggcagctgtg gtggggacat cgaatcccgg cctggtactg cccggacggc
      721 cacatcacgg tgtcagagtt gctcgaggga ccgcaggtct gtgccgactg cgtgcgccct
      781 cagtccgagc tgcgccagga agacgacata ttcgacacgt ggttctcaag cggcctgtgg
      841 ccgttctcca ctctgggttg gcccgatgac accgctgacc tgcgcaccta ctacccgacg
      901 accgttatgg aaacgggcta cgacattctc ttcttctggg tcgcccgaat gatgatgctc
      961 ggagaatggc tgaccgatcg ggcgcccttc gcaaccgtct atctgcacgg catcgttcgc
     1021 gacccgtatg gccagaagat gtccaagtcg aaaggcaacg tcctggaccc gttggcagtt
     1081 atagaggagc tgggtgccga cgccttgcgc ttctcccttc tccacgggcc ggagcccagc
     1141 caggaccagc ggatggcccg ccctcgattg gaggagtcgc gcaacttcgc caacaagatc
     1201 tggaatgcag cacgcttcgt gcactcggta cggccgcccg acgtgggctc ggatgccaag
     1261 ctcgggctgg ttgagattga ccagctgggc ccagccgagc actggatcct cgaccgctgc
     1321 gccggaactg ttgaagctgt ggaccgtgcc tacggcacct acgaattcgg tgaagtggcg
     1381 cggcttcttt acagcgcggt gtggagcgac ttctgcgact ggtatctcga gttggccaag
     1441 atcggattga acgacgagtc ggcgagcccg gcgcggaagc gcgcgatctg gtcgacgctg
     1501 acatgggcgc tggaccgcta cctgcggctg ctgcacccgg tgatgccgat gttgaccgag
     1561 gagatctggc agacgctgcc acacacagct gacgaccctg agctgctgat cgtcgcgccg
     1621 tggccaaggg cggaggacac gccgatgaag gcccagtcgc gcctcgctga cggcacggac
     1681 cagctcatcg acctcattac ccagatccga gccgcacgag cagagtcagg gatcgccgca
     1741 gccgactggc tcgatgctcg catctggtta ccggacggtg ccgcgcatga gacctattcc
     1801 tcccttgagg cggcgctgag ccgcctcgct cggatccggc cgcagctcgc tgcgaatcgc
     1861 gacgagcttg acggcgccgg ggcccagaac agcctggcca tcatcactcc gaccgtcgag
     1921 gcgcgcctga tgcgctccga cgctgaccga aaacgagaac gtgtgcgtct cgaaagggag
     1981 ctggcggcgg ccgatgccgc gctgcgcagc tccgacgcgc gcctcgccga tcccaatttc
     2041 acgtcgcgtg cgccagagac tgtcgtcgag caggcccgtg cgcgcgccga tgagcttcgc
     2101 aaccatgccg acgctcttcg agcgcggctc gacggaactg gagagggagc ctgatgccga
     2161 gcatcccgtt catcggtcgc aagaagaagc cggctcgcgg cggcccgccg cgtccccagc
     2221 tggctccgga ccagctcgag ctgaacgatg tgcagttgcg actccagtac cgggccgtca
     2281 gcagccacgg cgtgcgcatg ccgtctgtcc atgacaatcc ggtgcgcctg ccgagtctcc
     2341 tggaagggtt tgcgaccaca ccagtcgagc tgatcgagcc cttgccgctc gaattccagg
     2401 acgcagcgcc gtccatcgtc cgtccggaga gcgccctgca ctggatcaac gtccaccatg
     2461 gacgcacgcc gatcggtcgc cacgcgctga tgatcctcga gtcgctcgac tcgctcgacc
     2521 tcgcgtacga gacgcttgcc gtcgcgttgt tgtacggcga tgtcgacggc gagggctttc
     2581 cgcggtttga tgcgatcgtc ggtggcccgg tcagttactg ggatgagaac agcggcgacc
     2641 tcgtcgtacg cctcgtgctc gcgtggggcg gcgacggtgc ccgcggcgac acgcaccgcg
     2701 tcgcaacccg tctcatggcc cggcttctcg tgaatctgct ggccagccag ggcgccaccg
     2761 agctcggtcg cgtcgccaag cccgtggccg ttaccggcgg atctcagatc agatgctcac
     2821 actgtgggtt cgatgcgctc gatcgacgag cgcactattg ccccaagtgc gggatgcgca
     2881 cgggagcctg atgccgcttt acgactatga atgccggagt tgtggccatc gcgtggaggt
     2941 gctgcacggg gtaaacgcca cgggtccgca gtcttgcgag cgctgtggtg gcgccatgcg
     3001 caagc
//
LOCUS       DATLCO010000349         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6470680, whole genome shotgun sequence.
ACCESSION   DATLCO010000349 DATLCO010000000
VERSION     DATLCO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6470680"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2094
                     /gene="alaS"
                     /locus_tag="VMZ33_08080"
     CDS             <1..>2094
                     /gene="alaS"
                     /locus_tag="VMZ33_08080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HUR17230.1"
                     /translation="CIPDHDENCPRWLEIWNLVFMEFDQKPDGPRVPLPFKSVDTGMG
                     FERLTSVIQGVNTNYDTDCFTPIHEHMRAMLGHDPEGFEAERFSYQVIADHVRALTFL
                     VPDGIRPSNEGRGYVMRRLLRRAVRHGRLLGRTEPFLAELSGVVIDKMKVAYPYLEEW
                     RDQIHGVIAAEERQFARTLDAGIIQFEEALIPLTSADRVVGGKTADALPDDAPVLPGD
                     VAFRLHDTFGFPIDLTVELAAEYGVRVDRPGFETALEEQRQRSRGGRKQELARHAEMS
                     GLYQEIAARTSGGVQFLGYETTTTEARVVAILRDGTEYDQLEAVEEVELRVPAQAEAE
                     IVLDRTPFYAEGGGQVGDQGVLRMGVGVIFTVSDTQRPVAGLIVHRGTLHGRIAVGDE
                     VSAVVDPIRRARTMRNHTGTHVLHRALRNNVGERARQAGSLVTPDYMRFDFPFERALT
                     DEEKRSIEAEVRAVVRENKTVTPKYMSMSEAIDAGADAFFDEKYGEQVRVVFVDGFSR
                     ELCGGTHCSASGQIGSFVITGERSIGSGMRRIEALTGDAADSHLANRLALLDRVVEQA
                     GARDAESLPDRVRELQDRTKELERRVKAGGSAGQARPTDLARGAESLDGARFVAYSAP
                     FESMKELQSFARSLRAELGDGVIALALEADEPQIFVTVSDDLVARGVSAGALVSQGAP
                     VMEGRGGGRPEMAQAR"
BASE COUNT          357 a          672 c          718 g          347 t
ORIGIN      
        1 tgcatccccg accacgatga gaactgcccg cgctggctcg agatctggaa cctcgtcttc
       61 atggagttcg accagaagcc ggacggtccc cgtgtcccgc tgcccttcaa gagcgtcgac
      121 acgggcatgg gcttcgagcg cttgacgagc gtgatccagg gcgtcaacac caactacgac
      181 accgactgct tcacgccgat ccacgagcac atgcgggcaa tgctcggtca cgatccggaa
      241 ggcttcgagg ccgagcggtt cagctaccag gtcatcgccg atcacgttcg agcgctgacc
      301 ttcctggtgc cggatgggat acggccgtcg aacgaaggcc gcggctacgt gatgcggcgg
      361 ctccttcgac gcgcggtgcg gcatggccgc ctgctcggtc gcactgagcc gttcctggcc
      421 gagctgtcgg gcgtcgtgat cgacaagatg aaggttgcct acccgtacct cgaggaatgg
      481 cgcgaccaga tccacggcgt tatcgcagcg gaggagcgcc agttcgcgcg cacgctcgac
      541 gccggcatca tccagttcga ggaggcgctg atcccactga cctcggcgga tcgcgtcgtt
      601 ggtggcaaga cagcggacgc acttcctgac gatgcgccgg tcctgcccgg cgacgtcgcc
      661 tttcggctgc acgacacatt tggcttcccg atcgacttaa cggtcgagct ggcggccgaa
      721 tacggcgtgc gtgtcgaccg accaggattc gaaacggcgc tcgaggagca acgtcagcgc
      781 agccgcggcg ggcgcaagca ggagctggcg cgtcacgccg agatgagcgg gctgtaccag
      841 gagatcgccg cgcggacctc cggcggcgtg caattcctcg gctacgagac aaccacgacc
      901 gaagccaggg ttgtggccat cctgcgtgac ggaaccgaat acgatcagct ggaggcggtc
      961 gaggaggtcg agctgcgtgt gccggcgcag gctgaggcag agatcgtcct cgatcgaacc
     1021 cctttctatg ccgagggcgg cgggcaggtc ggtgatcaag gcgtcctgcg catgggcgtg
     1081 ggggtgatct tcaccgtctc tgacacccag cggcctgtgg ccggattgat cgtccatcgc
     1141 ggcaccctgc acggacgaat cgccgttggt gacgaggttt cggccgtggt cgatcccatc
     1201 cggcgcgcac ggacgatgcg caaccacaca ggcacccacg tcctgcaccg cgcactgcgc
     1261 aacaacgtcg gagagcgtgc ccggcaggcg ggctcgctgg tcactcccga ctacatgcgc
     1321 ttcgacttcc cgttcgagcg ggctctgacc gacgaggaaa aacgctcgat tgaggctgag
     1381 gttcgggccg tcgtccgcga gaacaagacg gtcacgccga agtacatgtc catgtccgag
     1441 gcaattgacg ccggcgccga cgccttcttc gatgagaagt atggcgagca ggtccgcgtc
     1501 gtcttcgtcg atgggttctc gcgtgagctc tgcggcggga cgcactgttc ggcatctggc
     1561 cagatcggct cattcgtcat caccggcgaa cggagcatcg gctcgggcat gcgccgcatc
     1621 gaggcgctga caggtgatgc cgccgattca catctggcca accgcctggc gctcctcgac
     1681 cgggtcgtcg agcaggccgg cgcgcgcgac gcggagtcgc tgccggatcg ggtcagggag
     1741 ctgcaggatc gaacgaagga gctcgaacgg cgagtcaagg ctgggggtag cgccggtcaa
     1801 gcacggccga ccgacctggc gcgtggcgcc gagtccctcg acggggcccg attcgtcgcc
     1861 tactcagctc cattcgagtc catgaaggag ctccagagct tcgctcggtc ccttcgggcc
     1921 gagctgggtg acggcgttat cgccctggcg ctcgaggcgg atgagccgca gatcttcgtc
     1981 acggttagcg acgacctcgt tgcccgcggc gtttccgccg gcgcgctggt cagccagggc
     2041 gcaccggtga tggagggtcg tggcggaggt cgaccggaga tggcgcaggc tcga
//
LOCUS       DATLCO010000350         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_11099298, whole genome shotgun sequence.
ACCESSION   DATLCO010000350 DATLCO010000000
VERSION     DATLCO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11099298"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VMZ33_08085"
     CDS             complement(<1..570)
                     /locus_tag="VMZ33_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUR17231.1"
                     /translation="MTAINGDWLPKGWRTYDDVHGRKNTVTGTVLVWPKLASKDLDRS
                     AEISVFLPPSLAKALKAETRNVRRYPVFYFHDGQNVFDDHTSHAGEWRVDETLQALAR
                     DGIEAIAVGIPNGHEARFDEYNPYKGRTPYTGRRVVGGKANEYLDWLTGEIKPLIDRT
                     FPTDPSRDATGTIGSSLGGLISLYAMVRHP"
     gene            complement(567..1394)
                     /locus_tag="VMZ33_08090"
     CDS             complement(567..1394)
                     /locus_tag="VMZ33_08090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HUR17232.1"
                     /translation="MILSRHDDELSIAVDAARRAGRIQMERYERLERIVHKSEHDVVT
                     EVDEMSEQLIISSIRSAYPHDGFLAEESGTTAAIAPVSQSGVESDVDPAAQRVWIIDP
                     LDGTVNYANGIPVFCVSIGLVIGGQPVLGVVYDPTRDELFTAQAGHGARLDDQPIRQP
                     QKEKLSDYVVSIALSRVGWSDRERKVRRRIRVSRSMGSAALALAYVGNGRFDAFIQSG
                     GLSLWDICAAGLIAVEGGATVTTLDGGPWFDMAQPSKSIGLVAAAPAHHATLKELIR"
     gene            1469..>2047
                     /locus_tag="VMZ33_08095"
     CDS             1469..>2047
                     /locus_tag="VMZ33_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR17233.1"
                     /translation="MTTEKRWTLICAVLGSSIVFLDSSVLPVALNAIGREPRLFVDVL
                     EGQNYIQYGYLLSLSALLILAGALSDFLGRKRLFVIGLVGFGVTSLLCGLAPNIETLI
                     IARVLQGATGAILVPGSLAILTTSFEGEEQGRAFGIWAGASGVAPILGPFVGGLLVDE
                     VSWRAIFLLNLPFIAVAVWAARRYVKESFDEKA"
BASE COUNT          327 a          663 c          676 g          381 t
ORIGIN      
        1 ggggtggcgc accatcgcgt acaggctgat caagcccccg agcgacgagc cgatcgtgcc
       61 ggtcgcgtcg cgcgagggat cggtcgggaa ggtcctgtcg atgagtggct tgatctcacc
      121 ggtcagccag tcgagatact cgttggcctt gccaccgacg acacgccggc cggtgtacgg
      181 cgtgcggccc ttatacgggt tgtactcgtc gaaacgcgcc tcgtggccgt tggggatgcc
      241 cacagcgatg gcctcgatcc cgtcgcgcgc caacgcctgg agcgtctcgt cgacccgcca
      301 ctccccggcg tgcgaggtgt ggtcgtcgaa aacgttctgg ccgtcgtgga agtagaagac
      361 cgggtaacgg cggacgttcc tggtttcggc cttcagcgcc ttggccaacg acggcggcag
      421 gaacaccgag atctcagccg agcggtcgag gtccttgctg gcaagcttcg gccacaccag
      481 gaccgtgccc gtcaccgtgt tcttgcggcc atgaacatcg tcgtatgtgc gccagccttt
      541 gggcagccaa tcaccgttga tcgcggtcaa cgaatgagct ccttgagcgt ggcgtgatgc
      601 gcgggcgcag cggcgacgag gccgatcgac ttgctgggtt gcgccatatc gaaccacggt
      661 ccgccgtcga gcgttgtcac cgtcgccccg ccctccaccg cgatgagccc cgcggcgcag
      721 atatcccaca gcgacaggcc gcccgactgg atgaatgcat cgaagcggcc gttgcccacg
      781 taggcgaggg caagcgcggc gctgcccatg ctccgggaga cccgtatacg gcggcgcacc
      841 ttccgctcgc ggtccgacca gccgacgcgc gacagtgcga tggagaccac gtaatcggac
      901 agcttctcct tctgcggctg gcggatgggc tggtcgtcca gccgagcgcc gtgcccggct
      961 tgcgcggtga acagttcgtc ccgggtcggg tcgtagacga cgcccagcac cggctggccg
     1021 ccgatgacaa ggccgatgct cacgcagaaa acaggtatcc cgttggcgta gttcacggtg
     1081 ccgtccaacg ggtcgatgat ccagacgcgc tgcgctgcgg gatctacatc ggattccacg
     1141 cccgattggc tgaccggcgc gattgcagcg gtagtccccg attcctccgc caggaacccg
     1201 tcatgcgggt acgccgagcg gatgctcgaa atgatcagct gctccgacat ctcgtcgacc
     1261 tcggtcacga cgtcgtgctc gctcttgtgg actatccgct cgaggcgctc gtaacgttcc
     1321 atctggatac gtcccgcgcg tcgagccgca tcgacggcta tggatagctc gtcgtcgtgt
     1381 cgactcaaaa tcacaggcgg cgatggtagc gacggatcag gaggtgggcc gatctgtacg
     1441 atcggggcgt ggccccacga gtagatccat gacgacggag aagcgctgga cgctcatctg
     1501 cgcggtgctc gggtccagca tcgtcttcct cgattcgtcc gtgttgccgg tggcattgaa
     1561 cgcaatcggc agagagccac ggctattcgt cgacgtgctc gagggccaaa actacatcca
     1621 gtacggctac ctgctgtcct tgtcggcgct gctcatcctc gccggcgccc tgtccgactt
     1681 ccttggccgg aagcggctat tcgtgatcgg actcgttggg ttcggcgtca catcgttgtt
     1741 gtgcgggctg gcgccgaaca tcgagacgct catcatcgcc cgggttctcc agggcgccac
     1801 gggcgcgatc ctcgtcccag gctcgctcgc gatcctgacg acttcgttcg aaggcgagga
     1861 acagggacgt gcgttcggca tctgggccgg tgcgtccggc gtcgcgccga tcctcggacc
     1921 gttcgtcggc ggcctgctcg tcgacgaggt gagctggcgc gccatcttcc tgttgaacct
     1981 gccgttcatc gcggtcgccg tgtgggcagc ccggcgctat gtgaaggaaa gcttcgatga
     2041 gaaggcg
//
LOCUS       DATLCO010000351         2056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_11121223, whole genome shotgun sequence.
ACCESSION   DATLCO010000351 DATLCO010000000
VERSION     DATLCO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11121223"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VMZ33_08100"
     CDS             <1..211
                     /locus_tag="VMZ33_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HUR17234.1"
                     /translation="GYERGAITPFGSSNAWPVVADAAILAQQLVAIGGGRRGVQIHIA
                     PDALVRATNADVVDVTDLELPGPPP"
     gene            complement(269..1375)
                     /gene="greA"
                     /locus_tag="VMZ33_08105"
     CDS             complement(269..1375)
                     /gene="greA"
                     /locus_tag="VMZ33_08105"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HUR17235.1"
                     /translation="MSAPELLRSAGLTVDGPVVWGSQIASQPSGVFVVEAPSPSTEAP
                     LDHKVIRGWLERVPGLRLDGKLPTPHTLAEHLATLWLPDVPVLYIGRSTRALSARIAS
                     MYATPLGDSKPHPGGYLLKTLSTLSHLRIWWAETEAHEEFEDALLAGFAKLAKGDAVP
                     FANAKPRLVNQLREPPARSAATQQADLRASAQKERRAVARGTRRTAVRVVAGVRPATA
                     PNYVSQEGRDQLAAELDNLRLVVRPEVIARVKAAREHGDLKENAEYEYARKEQSFVEG
                     RIQQIEGQLRNAVVVDESASTDSVGLGSTVVVEVDGDEERYVVVSQLEADPARGRISN
                     VSPVGRALFGRRAGEAVIAVAPGGEVTYLIKQID"
     gene            1440..>2056
                     /locus_tag="VMZ33_08110"
     CDS             1440..>2056
                     /locus_tag="VMZ33_08110"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17236.1"
                     /translation="MAIASPKRVIQAPRGTTISCRGWQQEAALRMLMNNLDPEVAERP
                     EDLVVYGGTGRAARSWEAFDAIVRELRRLGDDETLLVQSGKPVGVFRTHEWAPRVLIA
                     NSYLVGKWANWDTFRELERAGLTMYGQMTAGSWIYIGTQGILQGTYETFAELARQHFG
                     GTLRGRIVLTAGLGGMGGAQPLAVTMNEGVALVIEVNESTARRRM"
BASE COUNT          338 a          683 c          693 g          342 t
ORIGIN      
        1 cgggtacgag cgcggcgcca taacgccctt cggatcgagc aatgcctggc cggtcgtcgc
       61 cgacgcggcc atcctcgccc agcagctggt cgccattggc ggtgggcgac gcggcgtcca
      121 aatccacatc gcgcccgacg cactggtccg agccacgaac gcggacgtcg tcgacgtcac
      181 ggaccttgag ttgccgggtc cgcctccctg atcgacgtgt cctgcggcgg tgacccagcg
      241 gccattgccg atttttggcg gcgacgcgtc agtcgatctg cttgatcaag taggtgactt
      301 cgccacccgg ggcaacggcg atgaccgcct cgcctgcgcg gcggccaaac agcgcgcgtc
      361 cgaccggtga cacgtttgag atgcggccac gtgcggggtc agcttcgagc tgactcacga
      421 cgacgtagcg ttcctcgtcg ccgtccactt cgacgacgac cgtggaccca agaccaaccg
      481 aatcggtcga cgctgactca tccacgacga cggcgttccg aagctggccc tcaatctgct
      541 gaatgcggcc ctcgacgaac gactgctcct tccgcgcgta ttcgtactcg gcgttctcct
      601 tgagatcgcc gtgctctcgc gccgctttca ctcgcgcgat cacctctggt cgcactacga
      661 gtcgcaggtt gtcgagctcg gcggccagtt ggtcgcggcc ttcctgcgag acgtagttcg
      721 gcgcggtggc aggcctcacg ccggcgacga cgcgtactgc cgttcggcga gtcccgcggg
      781 cgacggcacg gcgctccttc tgcgccgacg ctcggagatc agcctgttgg gtcgctgccg
      841 accgtgccgg tggttcgcgc agctgattca caaggcgcgg cttggcattg gcgaacggga
      901 cggcgtcacc cttggccagt ttcgcgaagc cggccagaag ggcgtcctcg aactcctcgt
      961 gtgcctctgt ctcagcccac cagatgcgga ggtgcgacag ggttgagaga gtcttcagca
     1021 ggtagccgcc gggatgtggc ttggagtccc caagtggcgt cgcgtacatc gaagcgatgc
     1081 gcgcggacag cgcccgcgtg gaacgaccga tgtagaggac cggcacatca ggcagccaca
     1141 gcgtcgccag gtgttcggcc aacgtatgcg gggtcggcag cttgccgtcc aaccggagcc
     1201 cgggcacgcg ttcgagccaa ccgcgaatga ccttgtggtc gagcggcgcc tccgtcgagg
     1261 gactcggcgc ctcgaccacg aatacgccgg acggctggct ggcaatctgg cttccccata
     1321 cgaccggccc gtcaaccgtc aaacccgcgc tgcgcaacag ctccggggca ctcattcgat
     1381 cattcatcac gtccaccgta ctagggcgcc caagaatcgg cggctttaga ctcggctcga
     1441 tggcaatcgc ttcgccaaaa cgcgtgatcc aggccccccg cgggaccacc atctcgtgcc
     1501 gcgggtggca gcaggaagcc gcgctgcgga tgttaatgaa caacctcgat ccagaggtgg
     1561 ccgagcgtcc cgaggacctg gtggtgtacg gcgggaccgg gcgcgcggcg cgcagctggg
     1621 aggcattcga cgccatcgtc cgtgagcttc gtcgactggg cgacgatgag acgctgctcg
     1681 tccagtcggg gaagccggtc ggcgtgttcc ggacacacga gtgggcgccg cgggtgctca
     1741 tcgccaactc gtacctcgtt ggcaagtggg ccaactggga cacgttccgc gagctggagc
     1801 gcgccgggct gacgatgtac ggccagatga cggccggctc ctggatatac atcggtacgc
     1861 agggcatcct ccaggggacg tacgagacct tcgcggagct ggcgcggcag cacttcgggg
     1921 gcaccctgcg cggacggatc gtcctgacag cgggcctcgg cggcatgggt ggcgcccagc
     1981 cactggcagt cacgatgaac gagggcgttg cgctcgtaat tgaggtcaac gagtcgacgg
     2041 cacggcggcg aatgga
//
LOCUS       DATLCO010000352         3056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_3700415, whole genome shotgun sequence.
ACCESSION   DATLCO010000352 DATLCO010000000
VERSION     DATLCO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3700415"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VMZ33_08115"
     CDS             <1..352
                     /locus_tag="VMZ33_08115"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HUR17237.1"
                     /translation="VFDLPGVVERPSAISVPGARALWLSEGAEGPPEAFMVGNEFAHL
                     HPAPDQSLHMMLPPALAEEAIRAGWAEQHPVARRGLISPNAVMVYAPRIDSEREVVEQ
                     LVRASYSFARGQVS"
     gene            364..1110
                     /locus_tag="VMZ33_08120"
     CDS             364..1110
                     /locus_tag="VMZ33_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HUR17238.1"
                     /translation="MAKILMLASFGLEIVECGGALAKALRAGDDVRAAVLMSRPESQS
                     QIREAARILGIADVEFLGVGNGEVVVTPEAKEPIVGLIRRTRPDVLIMQDPEHAQHDL
                     DPDRRMIALLYAEALAVAGREWRIDECGGHAPHPVPTIYYMTPSNPNCIVEISDTFAI
                     KQKALEALAFQMTFSAQSIKERASAEAIRHLVENFDELRDDDTRLGLALHQQFDRALA
                     VYHGLAGHSGAVLGEAYRREGAFVLERLTP"
     gene            complement(1170..1397)
                     /locus_tag="VMZ33_08125"
     CDS             complement(1170..1397)
                     /locus_tag="VMZ33_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17239.1"
                     /translation="MENDDLKERIHALETAQATQAATTAGMEATQTAVTAGMQAAQAA
                     AQVGTWAVMAAGSVSMVVGMFLGFAIVKARN"
     gene            complement(1620..2744)
                     /locus_tag="VMZ33_08130"
     CDS             complement(1620..2744)
                     /locus_tag="VMZ33_08130"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUR17240.1"
                     /translation="MNARTAPVAVSLALALALAAGACGSSISHEVARDKQIDKDIAAV
                     VATGVPGATLLVRDGGSTNKVAYGVAETTSKAQLNVDDRFRIGSLAKTYVSVVMLQLV
                     DEKKLGLDDSIEKFVPGMVPNGAKITVRQLLNHTSGIPNYEEHPSYMAPYMAGDLAHV
                     TTPAQLVAMGTSFGSWFAPGTSSRYSNTNYTIAGLIIEKVTGSTLGAQLDQRIFKPLK
                     LDSTSLPTTPEITGAHAHGYFVMGPQGAMDVTNFSPSIAWAGGGIVSTTTDVAAFYRA
                     LLEGRLLPPELMRQMMTTVVDDKNGLVYGLGIEKKELPCGTAWGHQGNFPGYFMEAFS
                     TADAGKQITTAYNLDSTSMDSAQGEATLKLRTDAFCGVKP"
BASE COUNT          470 a          992 c         1049 g          545 t
ORIGIN      
        1 agtgttcgac ctccccggcg tcgtcgagcg ccccagcgcc atctcggtgc cgggtgcccg
       61 agcgttgtgg ctgagcgagg gagccgaagg gccacccgag gcgttcatgg tcggaaacga
      121 gttcgctcat ctccaccccg cccccgacca gagcctccac atgatgctcc cgccggcgct
      181 cgccgaggag gcgattcgtg ccggctgggc ggagcagcat ccggtggccc gccgggggct
      241 catctcgccc aacgcagtca tggtctacgc gcctcgcatc gactcggagc gagaagtcgt
      301 cgaacagctg gtgcgggcgt cctactcctt cgcccgaggc caagtgtcat aggagtcgtc
      361 atcatggcca agatcctcat gctcgccagc ttcggactcg agatcgtcga gtgcggagga
      421 gcgctcgcca aagcgctcag ggccggtgac gacgtgcgcg ccgcggtgct catgagccgt
      481 ccggagagcc agtcgcagat ccgcgaggca gcgcgcatcc tgggaatcgc cgacgtggag
      541 tttctcggtg tcgggaacgg tgaagtcgtc gtcacgcccg aggccaagga gccgatcgtc
      601 gggttgattc gccggacgag gcccgacgtc ctgatcatgc aggaccccga gcacgcacag
      661 cacgatctcg atcccgaccg gcggatgatc gccctcctct acgccgaggc gctggcggta
      721 gccgggcggg aatggcggat cgacgagtgc ggtgggcacg cgccgcatcc ggtgccgacg
      781 atctattaca tgacgccgtc caacccgaac tgcatcgtcg agatcagcga caccttcgcc
      841 atcaagcaga aggcgctcga agccctcgct ttccagatga ccttcagcgc ccagtcgatc
      901 aaggagcgcg ccagcgcaga ggcgatccgt cacctcgtgg aaaacttcga cgaactccgc
      961 gatgacgaca ctcgcctcgg cctggcgctg caccagcagt tcgaccgagc gctcgccgtc
     1021 taccacgggc tggctggtca ctcgggggcg gtgcttggcg aggcgtatcg ccgtgagggt
     1081 gccttcgtcc tggagcgact aaccccgtaa ccaccggtgc gtagacctcg gcacccggat
     1141 gcctgttcgg cacccgggtg acccgggtcc tagttccttg ccttgacgat ggcgaagccg
     1201 aggaacatgc ccacgaccat gctcacgctg cccgcggcca tgacggccca ggtgccgacc
     1261 tgggcggcgg cctgcgccgc ctgcatgccg gccgtgacgg cggtctgcgt tgcctccatg
     1321 cctgccgtgg ttgcggcctg cgtggcctgc gccgtctcca gagcgtggat ccgttccttg
     1381 aggtcgtcgt tttccatgcg tttgctcgtc tctattcgtg tgtgggtgaa ccggcgtcgt
     1441 gatctgcgtc gcatgcttgc gacatgtgag atcactgacg ggtacggccg ccctacccgt
     1501 ggttccgcgc actgaaacga gcgcgctccg cctgctgcgc ctctcagcac gacccaaaag
     1561 gctgcagccg tccgcagtgg cggacggctg cagccaagat cgatgcgggg ccggtagggc
     1621 tacggcttca caccgcagaa tgcgtcggtg cgcagcttga gcgtcgcctc gccctgggcc
     1681 gagtccatcg aggtggagtc gagattgtag gccgtggtga tctgcttgcc ggcgtcggcg
     1741 gtggagaagg cctccatgaa gtaccccggg aagttgcctt ggtgacccca tgcggtgccg
     1801 cagggaagct ccttcttttc gatgccgagg ccgtagacca ggccgttctt gtcgtcgacg
     1861 acggtcgtca tcatctgcct catgagctcc ggcgggagca accggccctc aagcagcgcc
     1921 cggtagaagg cggccacgtc ggttgtcgtg gagacgatgc cgccgccggc ccaggcgatg
     1981 gaggggctga agttggtcac gtccatggcc ccctgcgggc ccatcacgaa gtagccgtgg
     2041 gcgtgggcgc cggtgatctc gggcgtggtc ggcagcgagg tcgagtccag cttcaggggc
     2101 ttgaaaatgc gctggtcgag ctgagcaccg agggtggacc cggtgacctt ctcgatgatg
     2161 agcccggcga tggtgtagtt ggtgttggag tacctcgacg acgtgccggg ggcgaaccac
     2221 gagccgaatg acgtccccat ggccacgagc tgggccggtg tcgtcacgtg ggcgaggtcg
     2281 ccggccatgt acggggccat gtaggagggg tgctcctcat agttggggat gccgctcgtg
     2341 tggttgagca gctggcgcac ggtgatcttg gcgccgttgg gcaccatgcc cggcacgaac
     2401 ttctcgatgc tgtcgtcgag tccgagcttc ttctcgtcga cgagctgcag catcacaacc
     2461 gacacgtacg tcttcgccag gctgccgatg cggaatctgt cgtcgacgtt gagctgagcc
     2521 ttgctggtgg tctcggcgac gccgtacgcc accttgttgg tgctgccgcc atcgcggacc
     2581 aacaaggttg cgccgggtac ccccgtggcc accacggcgg cgatgtcctt gtcgatctgc
     2641 ttgtcgcgtg ccacctcgtg ggagatcgac gagccgcagg cgccggcggc gagagcgagg
     2701 gcaagggcga gtgaaacagc gacgggtgcg gtccgagcgt tcattgcatt cctccagagt
     2761 tgagatcgtg agctcactct ggaggccggc gctgttccgg cgctgttgcc gcgctgtcgt
     2821 caggtgggtg ggaggtgtcc tcagcgcagg aggcgaccac cgctcggcgg tcgcctcctg
     2881 gtcagcgtgc agctagccga cccgtcgctg gtccccgatt ggaacgccga gggcgggatt
     2941 ctgtcgttct tcgcgcaccg cgtgtgccac ctggagctgg cgcaggaggg tgacgaggcc
     3001 cacacctcca acgatgttgc ccaacgcggc ccaccccgcc gtctgtgccc aggccc
//
LOCUS       DATLCO010000353         4008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8901892, whole genome shotgun sequence.
ACCESSION   DATLCO010000353 DATLCO010000000
VERSION     DATLCO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8901892"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VMZ33_08135"
     CDS             <1..792
                     /locus_tag="VMZ33_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR17241.1"
                     /translation="LGVLIAAMATGLAREMGAQPGHVLTVVAAVTAAIAVGTIRSVRV
                     ARSRSDDQQAATEVAPSAARAILILAALCAAGFLVEDAIQNWSALHLERGLGASPALG
                     GAAPGVFAASMFIGRAMGHPLGSRVTERALLSGGAVVAAVGAAILAAAPSVLIAMVGL
                     AFSGAGISMVAPALFARAGRLAGPRGRGAAIARVTSLGYSGFIFGPAVVGLIAQATDL
                     RTALATLAVMALLVGIAGWLVMTGDSTEAKGTFDDGEELLRTGRA"
     gene            803..1234
                     /locus_tag="VMZ33_08140"
     CDS             803..1234
                     /locus_tag="VMZ33_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HUR17242.1"
                     /translation="MRFQRFGDRYQLRLESGDELLSTLTAFAERERIEYAAVSGLGAV
                     QSISLAYFVRNTKQYETHELDEQFELLGATGNIVLRDGTPFPHIHASVGRRDLTVLGG
                     HVMRAVAWPTIELWVSREHQPVNRLPDDESGLALMDLHDRL"
     gene            1247..2530
                     /locus_tag="VMZ33_08145"
     CDS             1247..2530
                     /locus_tag="VMZ33_08145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR17243.1"
                     /translation="MEPNVGSTPGRVVQVSISRGGVPKLPISVGQVGRLGVEGDAHHE
                     DTVHGGPHRAVCLLAMEAIERMQADGHPIGPGSAGENLTTTGIEWSLLPVGTRARIGD
                     MLEIELSDSTTPCSTQVANFSDGNFNRMNIVVHPSDSRMYARVVSDGPVRPGDEIRLS
                     PPLDGNAADELLLKRLDRAETKSSVAAWKAAKHAGFQIHVVEDGELAMSASPDIPGPA
                     FNQASGLARIPNLLSRATDFYDRQGTTGYVWLEAPPWPNAVVSLELGMFAGDPLAVPA
                     EAAPEGVLIRRIDPDEAERYTQVRSGSATAGGVTDGGPNPWPQVYAELARHNARQLFL
                     AEIDGRPVGNGSLHISARTGWLRGATVSPAARGRGIQRALVAARVAAAIAAGCDLVGA
                     SAESGTVSARNLERMGLRQVGRRSSYVYEPQPRLL"
     gene            2527..3339
                     /locus_tag="VMZ33_08150"
     CDS             2527..3339
                     /locus_tag="VMZ33_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUR17244.1"
                     /translation="MSDLLRVERTGARGAIVRVTLNRPEVHNAFNANLIGELRTTFLR
                     FGDEPAEQLRAVVLRGAGPSFCAGADITWMRASLGLSREQNEQDAMVMAEMFDAIDRC
                     PVPVIARVQGAALGGGMGLCAVSDLVIAEAGARFGFTETRLGILPAVISPFVIAKIGE
                     THARALFPSGRRFDATRALRIGLVHEVVEGEGALDAAIDTALSDLLAAGPTAARAAKA
                     IVREVRGLPHESTRWHTARRIATQRTSGEGQEGLKAFLEKRDPAWKEAISDD"
     gene            3385..>4008
                     /locus_tag="VMZ33_08155"
     CDS             3385..>4008
                     /locus_tag="VMZ33_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HUR17245.1"
                     /translation="MSVGTATVGPGEAARRLGVSTRTVQRWLREGRLPSVKVGSHVKV
                     PVHALPGGPSAQAPRQIGRLLVANRGELVVRIARTCRQLGIQSLALVPDDQARSWWIG
                     AADEVVPLDGSYLEADAVLAAARAARADAVHPGYGFLAENAEFAQAVIDAGMAWVGPP
                     PAAMRALGDKAAARELAQRLGVPVLPGYNGADQTNAKLAREAKRIGFP"
BASE COUNT          609 a         1346 c         1408 g          645 t
ORIGIN      
        1 ctcggcgtcc tgatcgcggc catggcgacg ggcctcgccc gcgagatggg cgcgcagccg
       61 ggtcacgtct tgacggttgt cgcggcggtg acggcagcga tcgcagtcgg cacgatcaga
      121 agcgtccggg ttgcccgcag caggagcgac gaccagcagg ccgccacgga ggtcgctccc
      181 tccgcggcgc gggcgatcct cattctggcg gcgctgtgcg cggccgggtt cctcgtcgag
      241 gacgcaatcc agaactggag cgcgctgcat ctcgaacgtg gccttggcgc gagtccggcg
      301 ctcggcggcg cagcgcccgg tgtctttgcc gcatccatgt tcatcggccg cgcaatgggc
      361 catccgctcg gctcgcgcgt caccgagcgg gcactcctgt cgggcggcgc ggtcgtggcc
      421 gccgttggcg ccgcaatcct ggcggccgcg ccgtcggtgc tcattgccat ggtcggtctg
      481 gcgtttagcg gcgccggcat cagcatggtt gcgccggcac tctttgcccg cgccggtcgg
      541 ctcgccggac ccaggggccg cggagctgcc atcgcgcgtg tcacttcgct tggctacagc
      601 ggcttcatct ttggcccggc agtagtcggg ctgatcgccc aggcgaccga ccttcgtacg
      661 gcgcttgcca cgctggccgt catggccctc ttggtcggca tcgccggctg gctggtgatg
      721 accggtgaca gcaccgaagc caagggcacg ttcgatgacg gcgaggagct gctccggaca
      781 ggtcgcgcgt aggatcggac gggtgaggtt tcagcggttt ggcgaccggt accagctgcg
      841 gctcgagtcg ggcgacgagc tgttgtcgac cctgactgcc ttcgctgaaa gggagaggat
      901 cgagtacgcg gctgtgtcgg ggcttggcgc ggtccagtcc atctcgctgg cgtattttgt
      961 ccgcaacacg aagcagtacg agacccacga gcttgacgag caattcgagc tacttggcgc
     1021 gaccggcaac atcgtcttgc gcgacgggac gccgtttccg cacatccacg cgtcggtggg
     1081 ccgacgtgac ctgaccgtac tcggcggcca cgtgatgcgc gccgtcgcgt ggccgaccat
     1141 cgagctctgg gtcagccgcg agcatcagcc cgtgaatcgc ctgcccgacg acgagagcgg
     1201 gctggccctc atggacctgc acgaccggct atagcatcgg cggccgatgg aacccaatgt
     1261 cggctcaacc ccaggtcgtg tcgtacaggt cagcatctcg cgcggcggcg tgcccaagct
     1321 ccctatttcg gtgggtcaag ttggccgcct cggcgtcgag ggtgacgctc atcacgagga
     1381 cacggtccac ggcgggcctc accgggcggt ttgcctgctg gccatggaag caattgagcg
     1441 gatgcaggcc gacggccacc ccatcgggcc ggggagcgcc ggcgagaacc tgacgacgac
     1501 ggggatcgag tggtcgctgc tgccggtggg cacgcgcgcc cgaatcggcg acatgctgga
     1561 gatcgagctt tcggactcga ccacaccatg ctccacccag gtcgcgaact tcagcgatgg
     1621 caacttcaac cggatgaaca tcgttgtgca tccctcggac agccgcatgt acgcccgggt
     1681 cgtgtctgac ggccccgtgc ggccgggtga cgagatccgg ctcagcccac ccctggatgg
     1741 aaatgcagca gacgaattac tgctgaagcg tctcgaccga gcggaaacaa agtcaagtgt
     1801 cgcggcatgg aaggctgcca agcatgccgg cttccagatc cacgtggtcg aggacggcga
     1861 gttggcaatg tccgcatcgc ctgacatccc cggaccggcc ttcaaccagg catctggttt
     1921 ggcccgcatc cccaacctcc tttcccgcgc gaccgatttc tacgaccggc agggcacgac
     1981 tggatacgtc tggctggagg cgccgccgtg gcccaatgct gtggtttcgc tggagctggg
     2041 catgttcgcg ggcgacccgc tggcggtccc cgccgaagcg gctcctgagg gcgtgctgat
     2101 caggcgcatt gatcctgacg aggccgagcg ctacacacag gttcggtcgg ggagcgcaac
     2161 tgcgggcggg gtcacggacg gtggtccgaa cccgtggcca caggtctacg ccgagcttgc
     2221 gcgccacaac gcgcggcagc tgttcctcgc tgagatcgac gggcgccccg tcggaaacgg
     2281 ctcgctgcac atatctgcca ggactggttg gctgcgcggc gccacggtgt cccctgcagc
     2341 ccgtggccgc ggcatccagc gcgccctggt cgcggcacgc gtggccgcgg ccattgcagc
     2401 cgggtgcgac ctcgtgggtg cgtcggcaga gtctgggact gtgtcagcgc gaaacctcga
     2461 acgaatgggt ctgcggcagg tggggcgccg atcgagctac gtctacgagc cgcagccgag
     2521 gttgctgtga gcgacctcct ccgggtcgag cggaccggcg cacgcggtgc catcgtccgc
     2581 gtgacgctca accgtcccga ggttcacaac gcgttcaacg cgaacctcat cggagagcta
     2641 cggacgacgt tcctgcgctt tggtgacgag ccagctgagc agttgcgcgc cgtcgtgctg
     2701 cgcggtgccg ggccgtcctt ttgcgccggc gccgacatca cgtggatgcg cgcctcgctc
     2761 ggcctgtcgc gcgagcagaa cgagcaggac gcgatggtca tggccgagat gttcgacgcc
     2821 atcgaccgct gtcccgtgcc cgtcatcgca cgcgtgcagg gagcggcact cggtggcggg
     2881 atgggtctct gcgcggtgag cgacctggtc atcgccgagg ctggcgccag gttcggcttc
     2941 accgagacgc gcctgggcat cctgccggcg gtcatctcgc cgttcgtgat cgcgaagatc
     3001 ggcgagaccc acgcgcgagc cttattcccg tccggcagaa ggttcgacgc cacgcgcgcc
     3061 ctgcgcatcg gcctggtaca cgaggtcgtt gagggcgagg gtgcgctcga cgcggcgatc
     3121 gacacagcgc tgtctgacct tctggccgcc gggccgacag ccgcgcgggc ggccaaggcg
     3181 atcgtccgcg aggtgcgcgg acttccacac gagtcgacac gctggcacac cgcccggcgc
     3241 atcgcgacac agcgcacgtc aggagagggc caagagggcc tcaaggcctt tctcgagaag
     3301 cgcgacccgg catggaagga agcgatttcc gacgattgag ccgcattgtt tgacacataa
     3361 cgcgacaaac gcgacattca cgacgtgagc gttggtacag cgacagtcgg gccgggcgag
     3421 gcggcgcgcc ggcttggcgt cagcacccga acagtccagc gctggctgcg cgagggtcgg
     3481 ctcccttctg tcaaggttgg ttcccacgtc aaggttcccg tccatgccct gcccggcggc
     3541 ccgtccgctc aggcaccgcg gcagatcggg cggctgctcg tggcgaaccg cggcgagctc
     3601 gtcgtacgga ttgcccgcac gtgccgtcag ctcgggatcc agagcctggc cctcgtgccg
     3661 gacgaccagg cacgctcgtg gtggatcggc gcggccgatg aggtcgtccc gctagacggg
     3721 tcgtacctgg aggcggacgc ggtccttgcc gccgcccgtg ccgcgcgcgc ggatgcagtc
     3781 caccccgggt atggcttcct tgccgaaaac gccgagttcg cgcaggccgt gatcgacgcc
     3841 ggcatggcat gggtcggccc gccaccggcc gcgatgcggg cgctcggcga caaggcagcg
     3901 gcacgggagc tggcccagcg gctgggggtg ccggtgctgc ccggctacaa cggcgccgac
     3961 cagaccaacg ccaaactggc gcgcgaggca aaacgcatcg gcttcccc
//
LOCUS       DATLCO010000354         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10233347, whole genome shotgun sequence.
ACCESSION   DATLCO010000354 DATLCO010000000
VERSION     DATLCO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10233347"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..363
                     /locus_tag="VMZ33_08160"
     CDS             163..363
                     /locus_tag="VMZ33_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17246.1"
                     /translation="MTTSSSSVWRRSVPKDAPPEPFKHLIDRYQHREQLVTCTCGWQG
                     SSSDTVPDGWKAHLLEVRGPSR"
     gene            complement(435..1835)
                     /locus_tag="VMZ33_08165"
     CDS             complement(435..1835)
                     /locus_tag="VMZ33_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17247.1"
                     /translation="MTVRSAIGPAITDWRRFGAPGFRVFHYLALCQGVVGVLAGFDVV
                     IPFALAVGVPPFAAVLLGILPLAGGMAQLVMPRLLDRTDGNLRGLTILFATIAEPNGL
                     YFALLAVMVGLGIVHGPLVIVMLATLVGVSSLLRSMVSSNLLTWYSAVLPEQERRLVV
                     PRMFALSLGIGALLLLPMAMVLDRLVDAVGVLAYALPFAVSGVLGVAEVFVLRRMRHP
                     GRVIVPPAATKEPDKSAGADTVRDPGARPEFRAFLRVSALNALGMGFAPSMSVFIISI
                     LGLSAGFSMTVGAIGTLTMVGAAAFFGARLAHGSSQTMLRHSFWMRAAAMALPLLALP
                     GTMWAPFLMIGTTMLASAGFAAGQLSSNERLFRLIQGPAVVRHHARYLARTSGAMTVG
                     QTIGAGILAVAMPLGYPAFAILYATSSTLRVLAFRLSRPAAVLPFTEPEPEPVPPREP
                     LPAGQTEAAETVASAA"
BASE COUNT          442 a          716 c          759 g          299 t
ORIGIN      
        1 ggttggggcc aaatcgatta gaatgtgaga gcccattcgg gcaacgctcg cagtctcggc
       61 agcccagccg tcgcgggttc cgtgcatatc gcccgcgtcg gatgccctcc gcgtcacggt
      121 ggctcgcgtg cgtcaacgtc gtctgagtga ggtccatccc gcgtgacaac ttccagctct
      181 tccgtctggc gccggtccgt ccccaaggac gccccgcccg agcccttcaa gcacctgatc
      241 gaccgctatc agcaccgcga gcagctcgtg acctgcacgt gcggttggca gggcagcagc
      301 tcggacacag ttccggatgg ttggaaggcg cacctgctcg aggtccgcgg cccctcacgc
      361 tgataggccc ctcacgctga tacttgagcg acgcccgcgc aggtgcgggc tcgcgacgtc
      421 tggataacga acgctcaagc ggcggaggcg acagtctccg ccgcttctgt ttgtcccgcg
      481 gggagtggct cgcgcggtgg cacgggctcg ggctcgggct ctgtgaatgg caagacggca
      541 gccggacgcg aaaggcggaa ggccagcacc ctcagcgtgc tggacgtcgc gtacaggatg
      601 gcgaacgccg ggtagccgag tggcatggcg acggccagga tccccgcgcc gatcgtctgg
      661 ccaaccgtca tggctccgga ggtacgggcc agataccgcg cgtggtggcg gaccaccgca
      721 ggtccctgga tcaggcgaaa gagccgctcg ttgctggaca gctggccggc cgcgaagccc
      781 gccgaggcaa gcatcgtcgt gccgatcatc aggaagggcg cccacatcgt gcccggcagg
      841 gcgagcagcg gcagggccat cgcagcggca cgcatccaga acgagtggcg cagcatcgtc
      901 tgcgaggacc catgggcgag gcgcgctcca aagaacgctg cggcgccgac catggtcaac
      961 gtgccgatcg cgccgacggt catcgagaag ccggcagaga ggccaaggat cgagatgatg
     1021 aacaccgaca tcgacggcgc gaagcccatg cccagcgcgt tcagcgcact cacccgcaga
     1081 aacgcacgga actctggcct ggcccccgga tcccggacgg tgtcggctcc agcggactta
     1141 tcgggctcct tggtggccgc cggcgggacg atgacccggc ccggatgccg catgcgacgc
     1201 agaacgaaga cctcggcgac acccaggacg ccggagacgg cgaaaggcag agcgtaagcg
     1261 aggacgccga cagcatcgac cagccggtcg agaaccatgg ccattgggag caggaggagg
     1321 gccccgatgc cgagcgacaa cgcgaacatc cgtgggacga ccagccgtcg ctcctgctct
     1381 ggcagcacgg cggagtacca ggtgaggagg ttggagctga ccattgaccg caggagtgag
     1441 ctgaccccga ccagcgtggc aagcatcacg atcacgagtg gtccgtgcac gatgccgagc
     1501 cccaccatca cagcgagcag agcgaagtag aggccgttgg gctcggcgat ggtggcgaac
     1561 aggatggtca gtccgcgaag gtttccgtcc gtccgatcga gcaggcgcgg catgacgagc
     1621 tgcgccatgc cgccggcgag cggcaggatg cccagcagaa ctgccgcgaa gggcgggaca
     1681 ccgactgcaa gagcgaacgg aatgacgacg tcgaagccgg ccaggacacc gaccacgccc
     1741 tgacacagcg ccaggtaatg gaagacgcgg aaccccgggg cgccgaaacg acgccagtcg
     1801 gtaatagccg gtccaatcgc ggatcgaacg gtcacggaga aggagcctca gggttctcac
     1861 gtccccagag tcagccgatc cgatcgtcgc ctgagatcgt cgggctctct tctcgccgtc
     1921 acgcctttga acaggcgccg catggaacgc gggatgacaa ccgcgctcgc gagactccag
     1981 tggaatcggc cctcagttta gccagtatgc ctgtggagcg ccgcggccag acgtcctgat
     2041 ctcagccgct aatgtgagcg aatgtgcaga agcatcatgc gattgaggga ccaggacgta
     2101 ccagccaccg acgaggagat ccgcgctgcg gcgctccagt tcgtgcgcaa gataagcggc
     2161 tttcggcaac cgacgaagcg acacgaagag gccttccagc gtgcggtgga ggagat
//
LOCUS       DATLCO010000355         5659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_7450688, whole genome shotgun sequence.
ACCESSION   DATLCO010000355 DATLCO010000000
VERSION     DATLCO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7450688"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VMZ33_08170"
     CDS             complement(<1..822)
                     /locus_tag="VMZ33_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUR17248.1"
                     /translation="MTLRTRPTRPTSRVTRFRKLFDDEDRQQAVVSAAFILAIVAVVA
                     LLLAAIGIAYYNDNIRPLARVGSTEVGPQLLRDRVEMEAWRIQRETNRLTQASINGDI
                     DEATRSRRAALLEQRQENLSTTSVEDLIDLLYQSQLATEEGITVADSDVDAAVAREFN
                     DIEKRHVWAIIIKPGDGKAAEDVTNADRQAAIAKAEEALHALEAGTPFDEVAREFSTD
                     ATKLNGGDLGVVTELALEEEWSAELFELPLDGTTGIIRGADGTFRIGRVTEIIEAG"
     gene            complement(835..1482)
                     /locus_tag="VMZ33_08175"
     CDS             complement(835..1482)
                     /locus_tag="VMZ33_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17249.1"
                     /translation="MSDYQNAEHSNGAPAEDWPTGDQPLAADDSDAPVSLAAASDDAP
                     TLGEALDASAELNAAVAGTDAEAAIANFETVSSDDAAGLGDIGPSDSVEIRNGGARDI
                     DATTVSITQGGARDIDAQNVTITQGGAAQVHADDLTINQGGVAIARTENLRIAEGGSA
                     FAVVADKATLSSESSVFLLIAGSSSGDVRPVLDWRTAAALGAGFVLAISLIRRLR"
     gene            complement(1573..2847)
                     /locus_tag="VMZ33_08180"
     CDS             complement(1573..2847)
                     /locus_tag="VMZ33_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HUR17250.1"
                     /translation="MARLEAAATTKTQAPEPRGVRVVIDVRPLQEPERSPLTAAYLDS
                     LLRAFAAAPREGESFVLVTRTLRDDPTERLAAAGLPIAGLRRLPPTTRALRSAGLTLD
                     SFLLRGAEVGVAVADEPAVFHTAGGAVPLASRLPVVATLLDLAPWELPETYAATAAAR
                     FGHRLRARVLHDAACVIVCSRAVGDEARRHLHLPPEKMSVIPLAVDDDFRAAGRDEAA
                     VAATRTRLGLPDRYLAFAGRYDARKDVKTLFEALAAIRDEGKPEPAVVFAVDAAHRED
                     ADLVIRGAARYGLVETVKPVIVDGVADRAALIAGARAFVYPAVSEATALPVLEALSLG
                     VPVICSRIGALPEAVASSGIVVSPRDAARLAAAIEAIWSGGALAEQLRTQGRNRARTR
                     VRTWADVARETRAAYAAAAFPTTPPFLLGPAS"
     gene            complement(2847..3635)
                     /locus_tag="VMZ33_08185"
     CDS             complement(2847..3635)
                     /locus_tag="VMZ33_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HUR17251.1"
                     /translation="MTSEPAYADVWVVLPTYNEKENLPQIAPAILQQLPGATLLVVDD
                     SSPDGTGELADAMSAENPLVRVLHRPGKQGLGKAYVDGFSFALEGGAARVVQMDADWS
                     HDPKYLPGLVGGLRSTAEASDGADLVIGSRYVKGGGVRNWSLPRRIISRGGSLFARVV
                     LLLGPHDLTGGFKAWRREILGALPWERMHSGGYVFQIETTFLASRIGAKVAEIPIVFV
                     DRVLGASKMSRRIIGEALIVVLQLRWDALRGRIPRQPRSANNEG"
     gene            complement(3760..5550)
                     /locus_tag="VMZ33_08190"
     CDS             complement(3760..5550)
                     /locus_tag="VMZ33_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HUR17252.1"
                     /translation="MRSFRVVFMAFALVFTGAVAAATPAQTRAAAAPLKAVIIVGPAG
                     DLTAQDLEDAESLAVVAENMGMDVRRVFHPHATWENVMANIQGANLVYYAAHGYGWPS
                     AYTQVMTESRQNGVGLNSFDGSSVNQYTYYGANVIRANWVLAPNSIVFLNHDCYTAGN
                     AEPGMAIPGWDVARQRVDNFAAGFLAVGAKAVFAYSGQRFNKTVQQLFTTDMTVEQMF
                     RTVGTKPHPYYGWVGWDARKFDSVRMPGNQNFMDPHSTLGFLRAVSGNLSLTAAQWAA
                     GFGDGGDPPFISNLAATAPAGPQFGSVTTPFFTPNSDGVTDSMTLRYTVDKEAFVDLE
                     VRNAGGTIVRTLSGWSPGGGGTAVWDGKNSAGGYVTDGTFTLTATPRNRAGATGNSES
                     MDVSVLTVMSQPNAAPAMFYPTDNDNLAPVSNLSVTLDEAASFWWKISDSNGNPVRTH
                     MNSVAVDAGPQTFAWDGKDNAGAYVPDGVYYSVTTTSTAAGLYYHSVPVEVRAFRATT
                     AAQSPFTRGTKTRVTIASAEPMKAKPKVKVFLPGKTPMTYYTTAMAGGGWYTTVSFPL
                     TAQPGTVTIQVFGPDANGIQNKSTYTYQLN"
BASE COUNT          914 a         1830 c         1964 g          951 t
ORIGIN      
        1 gccggcctcg atgatctctg tcacgcgacc gatgcggaac gtgccgtcgg cgccgcggat
       61 gatgcccgtc gtgccgtcaa gcggcaactc gaacagctcg gcgctccact cttcctcgag
      121 tgcaagctcg gtcacgacgc ccaggtcgcc accgttgagc ttcgttgcgt cggtgctgaa
      181 ctcgcgcgcc acctcgtcaa atggagttcc agcctcgagc gcgtggagcg cctcctcggc
      241 ctttgcaatc gccgcctggc gatctgcgtt ggtcacgtct tcggcggcct tgccgtcgcc
      301 gggcttgatg atgatcgccc acacatggcg cttctcgata tcgttgaatt ctctggccac
      361 cgcggcatcg acgtccgagt cagcgaccgt gatcccctcc tctgtcgcca gctgcgactg
      421 gtagaggagg tcgatcagat cctcgactga ggtcgtggag aggttttcct ggcgctgctc
      481 gaggagcgcg gcgcgtcgtg agcgcgtcgc ttcgtcgatg tcgccgttga tgctcgcctg
      541 ggtcaggcgg ttcgtctcac gttggatgcg ccaggcttcc atctccacgc ggtcgcgcag
      601 gagctgcggg ccgacctccg tagagccgac gcgagccaag ggccggatgt tgtcgttgta
      661 ataggcgatg ccgatggctg ccagcaggag ggcgacgacg gcgacgatcg ccaggatgaa
      721 cgccgcgctg acaactgcct gctgccggtc ctcatcgtcg aaaagctttc ggaagcgcgt
      781 gacgcgcgag gtggggcgag ttggacgtgt gcgaagagtc attcgggcgg tggcctaccg
      841 aagccgacgg atcagggaga tcgccagcac gaaacctgcg ccgagcgccg cggccgtacg
      901 ccagtcgaga acgggcctca catcgcccga agaactgccg gcgatgagga ggaagacgct
      961 cgactccgag ctcaaggtag ccttgtcggc cacgaccgcg aaagcagaac cgccttcggc
     1021 gatcctcagg ttctcagtgc gcgcgatcgc aacaccgccc tggttgatcg tcaggtcgtc
     1081 ggcgtgaacc tgggccgccc caccctgggt gatcgtgacg ttctgcgcat cgatgtcgcg
     1141 cgcaccgccc tgtgtgattg agactgtagt ggcgtcaatg tcgcgcgctc cgccgttgcg
     1201 gatctcgaca gagtcgctcg ggccgatatc gccaaggccg gcggcatcgt ccgaggaaac
     1261 ggtttcgaag ttcgcgatgg ccgcctcagc gtccgtccct gcgacggcgg cattgagttc
     1321 agccgaggcg tcgagggctt cgccaagcgt cggcgcgtcg tcggaggcgg cagcgaggct
     1381 cacgggcgca tcggaatcgt cagccgccaa aggttgatcc ccggtcggcc aatcttcggc
     1441 gggcgcgcca ttgctgtgct cggcattctg gtagtcagac acggttactc cgcggtgagg
     1501 tgacaggttt gggggtcgcc cccgactgac cagcgtaacc cgatgggccg gatcgcgctt
     1561 cggacaaagc gctcatgagg ctggtccgag gaggaatgga ggggtcgtcg ggaaggccgc
     1621 agcggcatag gcggcgcgcg tctcgcgggc tacatcggcc caggtacgga ctcttgtccg
     1681 ggctcgattg cggccctgcg tccgcagctg ctcggcgagg gcgccgccac tccatatcgc
     1741 ctcgattgcg gccgccagtc gggccgcatc acgaggggag accacgatcc cggaactggc
     1801 cacggcctcc ggcagcgcgc cgatacgcga gcagattacc ggcacgccga gcgatagagc
     1861 ctcgagcacc ggcagcgcgg tcgcctccga gaccgccggg tagacaaatg cacgcgcgcc
     1921 agcgatgagc gctgcgcgat cggcaacgcc gtcgacgatg accggcttga cggtctcgac
     1981 gaggccatag cgagcggcgc cgcgaatcac gaggtcggcg tcctcacgat gcgccgcgtc
     2041 gactgcgaac acgaccgccg gttcaggctt cccctcgtcg cgtatcgccg ccagcgcctc
     2101 gaagagcgtc ttgacgtcct ttcgggcgtc gtaccgtcct gcgaatgcga ggtacctgtc
     2161 gggcaggccc aggcgggtgc gcgtagctgc gaccgcggcc tcgtctcgtc cggcggcgcg
     2221 gaaatcgtcg tccacggcga gcgggatgac gctcatcttc tcgggtggca ggtgcaggtg
     2281 gcggcgcgct tcgtcgccga ctgcacgcga acagacgatc acgcatgcgg cgtcgtggag
     2341 gacgcgcgcg cggagccgat ggccaaagcg cgccgccgcg gtcgcggcat acgtctcagg
     2401 cagctcccat ggcgccaggt cgaggagagt cgcgacgacc ggcagccgcg aggcgagcgg
     2461 gaccgcgccg ccggccgtgt ggaagaccgc cggctcgtcg gcgacggcga cgcccacctc
     2521 agcgccgcgc aacaggaatg agtcgagcgt caggccggcc gagcgcagcg cgcgcgtcgt
     2581 gggcgggagc cgccgcaggc cggcgatcgg gagtccggcc gcggcgaggc gctcggttgg
     2641 atcgtcgcgc agggtccggg tgacgagtac gaacgactcg ccctcgcgcg gggccgcggc
     2701 aaacgctcgc aacaggctgt ccaggtaggc cgcggtcaac ggcgagcgct caggctcctg
     2761 gagcggtcgt acgtcgatga cgacgcggac tccgcggggc tcgggagctt gcgtcttcgt
     2821 tgtcgcggcc gcttcgagcc gcgccatcag ccctcgttgt tcgcgctgcg gggctgcctg
     2881 ggaatccggc cgcgcagcgc atcccagcgc agctggagca caacgatcag cgcttccccg
     2941 atgatccgac gtgacatctt gctggccccc agaacccggt cgacgaacac gattgggatc
     3001 tcagccacct tcgccccaat ccgcgacgcc aggaaggtcg tctcaatctg gaaaacgtag
     3061 ccgcccgagt gcatgcgctc ccacggcagg gcgccgagga tttctctgcg ccacgccttg
     3121 aagccgcccg tcaggtcgtg cggtccgagc agcagcacca cccgcgcgaa caacgagccg
     3181 ccgcgcgaga tgatgcgacg agggaggctc cagttacgga cgccgccacc cttgacgtag
     3241 cgcgacccga tcacgaggtc cgccccgtcc gacgcttccg ccgtcgagcg caagccgccc
     3301 acgagacccg gcaggtactt gggatcgtgt gaccagtcgg cgtccatctg cacgacccgc
     3361 gcagcgccac cttcgagcgc gaagctgaag ccgtcgacgt atgccttccc gaggccctgc
     3421 ttgcccggcc gatggaggac ccgcacaagg gggttttcgg cggacatggc atccgccagc
     3481 tcgccggtcc catcgggcga cgagtcatcg acaaccagca gcgtggcgcc aggcagctgc
     3541 tggaggatgg ccggggctat ctggggcagg ttctctttct cgttgtaggt cggcaggacg
     3601 acccaaacat cggcgtaggc gggctcggaa gtcacggcgg gagtctagcg ctggatatgg
     3661 cgaaagccgg ctctggggag ccgaccttcg cagagtgtgt ggcgggccag gggcacagcc
     3721 cgcgggtgca atcgacgtga agcggcagcg aggccgcggc tagttgagct ggtaggtgta
     3781 ggtggacttg ttctggatcc cgttggcatc cgggccgaaa acctggattg tcacggtgcc
     3841 cggctgggcg gtgaggggga aggaaacggt cgtgtaccaa ccgccgccgg ccatcgccgt
     3901 cgtgtagtag gtcatcggcg tcttccccgg caggaagacc ttgaccttgg gcttcgcctt
     3961 catcggctcg gcgctggcga ttgtcacgcg ggtcttcgtg ccgcgggtga acggtgattg
     4021 cgccgccgtt gtcgcgcgga aagcgcgcac ttcgacgggc actgagtggt aatagagccc
     4081 ggcagcggta gacgtagtcg tcacgctgta gtacaccccg tcgggtacat aggctcccgc
     4141 attgtccttg ccgtcccagg caaacgtctg cgggccggcg tctacggcga ccgagttcat
     4201 gtgggtgcgt accgggttgc cgttggagtc cgagatcttc caccagaacg aggccgcttc
     4261 gtcgagcgtg accgaaagat tgctgaccgg cgcaaggttg tcgttatcgg tcgggtagaa
     4321 catcgcgggc gccgcgttgg gctggctcat gacggtcagg actgatacgt ccatcgactc
     4381 ggagttgccc gtcgcgccgg cgcggttgcg tggcgtcgcc gtgagggtga acgtgccgtc
     4441 ggtgacatac ccgccggcgc tgttcttgcc gtcccatacg gccgtgccgc cgccgccagg
     4501 tgaccacccc gagagcgttc ggacgatcgt gccgccggcg ttcctgacct cgaggtccac
     4561 gaaagcttcc ttgtcgaccg tgtagcgcag tgtcattgag tcggttacgc catcgctgtt
     4621 gggcgtgaag aacggcgtag tcacgctccc gaactgtggg ccggcgggcg ccgtcgccgc
     4681 aaggttgctg atgaagggcg gatcgccgcc gtccccaaag ccggctgccc actgcgccgc
     4741 ggtcagcgac aggttgcccg agacggctcg caggaagccc agcgtcgaat gcgggtccat
     4801 gaagttctgg ttgccgggca tacgcactga atcgaacttg cgcgcatccc agcccaccca
     4861 gccgtagtac ggatgaggct tcgtaccaac cgtccggaac atctgctcga ccgtcatgtc
     4921 ggtcgtgaac agctgctgga cggtcttgtt gaagcgctgg ccggagtaag cgaacacagc
     4981 cttcgcgcca acggcgagga acccggcggc gaagttgtcg acgcgctggc gcgcaacgtc
     5041 ccagcccggg atggccatgc ccggctcggc gttgccggcc gtgtagcaat cgtggttgag
     5101 aaacacgatg gagttcggag ccaggaccca gttcgcgcgg atcacgttcg cgccgtagta
     5161 cgtgtactgg ttgacgctgc tgccgtcgaa tgagttgaga ccaacgccgt tctggcggct
     5221 ctcggtcatg acctgcgtgt aggcgctcgg ccagccgtag ccgtgggcgg cgtaatacac
     5281 gaggtttgcg ccctggatgt tggccatcac gttctcccac gtcgcgtggg ggtggaagac
     5341 gcgacggacg tccatgccca tgttctcggc cacgacggcg agcgattcgg cgtcctcgag
     5401 atcctgggct gtgaggtcac ccgccgggcc gacgatgatc acggccttca acggcgcagc
     5461 ggctgcccgc gtttgcgcgg gcgtagcggc ggcgacggca ccggtgaata ccagcgcgaa
     5521 cgccatgaaa acgacacgga acgagcgcat ttcccctctc tcttgaactg ttgctgggag
     5581 cacagactct tcgtgtagcg ctcgtcaaaa cattgcgaag aggtcctacc cgtcgattcg
     5641 ggcggagtgg ggtccagtg
//
LOCUS       DATLCO010000356         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10257657, whole genome shotgun sequence.
ACCESSION   DATLCO010000356 DATLCO010000000
VERSION     DATLCO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10257657"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2205)
                     /locus_tag="VMZ33_08195"
     CDS             complement(<1..>2205)
                     /locus_tag="VMZ33_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUR17253.1"
                     /translation="RSSFAEPTPAQAQGWPKIAGGDHTLICAPTGSGKTLAAFLWCLD
                     RLMSEPLPEDPLRRLRVLYVSPLKALVHDVNRNLRSPLAGIALAARNGGEASREIAVA
                     MRTGDTPADERRVFGKRPPDILVTTPESLYLLLTSQAREALRGVEWVIIDEIHALAGT
                     KRGAHLALSLERLEAMADKPPQRIGLSATQRPLGVVAGYLGGRQLPPEKSDAPGEPRP
                     VQIVDAGVRKELDIEVVVPVEDMGAIGEVLPIDQQPGGSAAAMDARSSIWPQIHPRIL
                     ELIRSHRSTIVFVNSRRLAERLALKLNELAGEELVRAHHGSLAREERLLIEEALKDGR
                     LPALVATSSLELGIDMGAVDLVIQVESPGSVARGLQRIGRAGHQVGEPSKGVLFPKYR
                     GDLLECAIVTRKMIDGEIEPTVIPRNPLDVLAQQIVATAAEHRWQTEDLYRLVRRAEN
                     FADLGRDSFEATLGMLAGHYPGDEFAELRPRVIWDRTAGTVESRRDARTIAVISGGTI
                     PDRGMFGVYLQEDGDGDRPGKTRARSAGSGGRRVGELDEEMVYEAREGEVILLGASAW
                     RIEQITHDRVLVSPAPGQPGKIPFWKGDGPGRPIELGRALGEFTRTISETAFENSRGR
                     KRALKLLQDDHELDELAANNLIDYLGEELSVTGALPTDRTIVMQRFRDELGDWRICVL
                     TPFGARVHAPWALAIEARLREQLGLEVQPIWSDDGIVVRLPMTDELGGEGLGD"
BASE COUNT          350 a          763 c          741 g          351 t
ORIGIN      
        1 gtcgcccagg ccctccccgc cgagctcgtc agtcatcggc agccgtacca cgatgccgtc
       61 atccgaccag atgggctgga cctccagccc gagctgctca cggaggcgcg cctctatcgc
      121 gagcgcccac ggggcgtgaa cacgggcgcc gaaaggcgtc aacacgcaga tgcgccaatc
      181 accaagctcg tctcggaagc gctgcatgac gatggtgcgg tcggtcggca gcgcacccgt
      241 gaccgacagc tcctcgccca gatagtcgat cagattgttc gcggccagct cgtcgagctc
      301 gtgatcgtcc tgcagcaact tgagtgctcg tttccggccg cgcgaattct caaacgccgt
      361 ctcagagatg gtgcgcgtga actcgcccag cgcgcgaccc agctcgatcg gccggcccgg
      421 cccgtcaccc ttccagaatg ggatcttgcc cggctggccc ggtgcgggcg aaacaagtac
      481 gcggtcgtgc gtgatctgct cgatccgcca ggcactcgcg ccgagcagga tgacctcgcc
      541 ctcgcgcgcc tcgtagacca tctcctcatc gagctcgccg acgcggcggc cgccactccc
      601 cgccgatcgg gcacgtgtct tgcccggtcg gtcgccgtcc ccatcttcct gcagatacac
      661 gccgaacatg ccgcgatcgg ggatcgtgcc gccactgatc accgcgatcg tgcgagcgtc
      721 gcgacggctc tccacggtgc ctgccgtgcg atcccagatg acgcgcggcc gcagctcggc
      781 gaactcatcg ccggggtagt gcccagcgag catgcccagg gtcgcctcga acgaatcgcg
      841 gccaaggtcg gcgaagtttt ctgcgcgccg cacgagccgg tacagatcct ctgtctgcca
      901 ccgatgctcg gccgcggtgg cgacgatctg ttgcgccagg acatcaagcg gattgcgcgg
      961 gatgacggtc ggctcgatct cgccatcgat catcttgcgg gtgacgatgg cgcactccag
     1021 caggtcgccg cggtacttcg gaaagagcac gcccttcgac ggctcgccaa cctggtgacc
     1081 ggcgcgaccg attcgttgaa gcccgcgcgc tacggagccg ggcgactcca cctggatgac
     1141 gaggtcgacg gcgcccatgt cgatgcccag ctcgaggctc gaggtggcca ccagcgccgg
     1201 caatcggccg tccttcagcg cctcctcgat gagaaggcgt tcttccctgg caaggctgcc
     1261 gtggtgggca cggacgagct cctcgccggc cagctcgttc agcttcagcg cgagacgttc
     1321 cgcgaggcga cgcgagttga cgaagacgat cgtcgaccga tgcgatctga tgagctcgag
     1381 aatccgcggg tggatctgcg gccagatgga gctgcgcgca tccatggcag cggctgagcc
     1441 gccgggctgc tggtcgatgg gcagaacctc gccgatggcc cccatgtcct caaccgggac
     1501 gacgacttct atgtcgagct ccttgcgaac tcctgcgtcg acaatctgga cggggcgcgg
     1561 ctcgccagga gcatcgctct tctccggagg cagttgtcgg ccgccgagat acccggcaac
     1621 gacgccgagc ggccgctgcg tggcggaaag cccgatccgc tgcggcggct tgtcggccat
     1681 cgcctcgagc cgttcgagcg acagcgcgag gtgcgcgccg cgcttcgttc cggcgagggc
     1741 gtggatctcg tcgatgatga cccactccac gccgcgcaat gcctcacgcg cctgcgaggt
     1801 gagcagcagg tagagcgact ctggcgtcgt cacgaggatg tccggcggcc gctttccgaa
     1861 gacgcgccgc tcgtccgcgg gcgtgtcccc ggttcgcatg gcgacggcga tttcgcgcga
     1921 ggcctcgccc ccgttacgtg ccgcgagtgc gatgccggcc aagggcgagc gcaggttgcg
     1981 gttcacgtcg tggacgagcg ccttcaaggg cgagacgtac agcacccgaa ggcggcgcaa
     2041 cgggtcctcg ggcagtggct ccgacatgag gcggtcgaga caccacagga aggccgcaag
     2101 tgtcttgccc gaaccggtcg gcgcgcagat cagggtgtgg tcgccgccgg cgatctttgg
     2161 ccagccctgg gcctgggcag gcgtgggctc agcgaacgag gagcg
//
LOCUS       DATLCO010000357         5065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8026847, whole genome shotgun sequence.
ACCESSION   DATLCO010000357 DATLCO010000000
VERSION     DATLCO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8026847"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..95
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            251..1246
                     /locus_tag="VMZ33_08200"
     CDS             251..1246
                     /locus_tag="VMZ33_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HUR17254.1"
                     /translation="MSRPTRVFLLSLALIIAACSGAPRPSATLHVDPTIPTAFPLGLR
                     DDEGTEVTITAEPQTIVSLTPANTEIVYALGAGDRLRGGTDFDDFPAEAAALPDVVAG
                     VQVLTEQIVAIDPDLVLAGGNNFTPAAEVERLRGLGVTVLVVYPETVDEVVADIGLIG
                     RAIGRHDEAMAITEGMEARIRDITSVVSRLDPLRTFYEIGYGPDIYGPAPNSFIADMV
                     ELAGGDAITSGDPAVFSISLEDLVDKDPEVIVLGDAAYGTCPETVPSRPGWDSMTAVA
                     ETAIRPVDDIVVTRPGPRLAEGLAALALAIHPDAQITPPTVGAVLCADPGVPSTP"
     gene            1243..2322
                     /locus_tag="VMZ33_08205"
     CDS             1243..2322
                     /locus_tag="VMZ33_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HUR17255.1"
                     /translation="MNAEATARRAAARPAWLGFLRHRPVALAVLGVGALCATLIAGVW
                     LGSVGIAPGDVLAILTHRLFRWPAEQTWPSSAETIVTELRLPRVLTAMAVGAALSVAG
                     ATYQGLLRNPLADPYVLGTASGAALGAAIAVLIPIQIGLLGLGMLHLFAFVGALLAVA
                     VVYRVSRLGALNSLTSVLLTGYAIGSLLAAGLAMAMYLSGNNLRQIFYYLLGSFSLAS
                     WPQLAVSALLIAIGLGLVLSRARSLNALLLGEEAAAHLGIDVRRDRLILLGGATLMTA
                     AAVAVSGLIGFVGLVIPHLVRLVVGPNARMLLPLSAIYGAAFLALADLIARAPGEVPV
                     GVVTALLGAPFFLYLLRRYRTGYEL"
     gene            2328..3077
                     /locus_tag="VMZ33_08210"
     CDS             2328..3077
                     /locus_tag="VMZ33_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR17256.1"
                     /translation="MALDVRNATVAYRGRVVVEDVSFEVGAGELVCLVGPNGAGKSTL
                     LRAISGTVALKSGIIAIDGVPRAEHDRVSLARRLAVVPGQTVIGFPMRVEELVALGRI
                     PHEHPLMGLRLADRAAIDHAIARVGIAHLRGRDVRELSLGERQLVVLAMTVAQGAQLL
                     LLDEPTVHLDLRHQVEVMQLLADLAQRDGVTVLTVLHDLSLASHFFPRLLLLDEGRLV
                     ADGPAGAVLTPDQVREVYRVDPRFVTVATSG"
     gene            complement(3092..4285)
                     /locus_tag="VMZ33_08215"
     CDS             complement(3092..4285)
                     /locus_tag="VMZ33_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HUR17257.1"
                     /translation="MTAGTPFHERTAPLNRKQSWREWSGYLAASAYDHLHIHEYSGIR
                     HAAVLIDVSPLYKYIVRGPDAARLIDRVITRDATKIQPGQVIYTPWCDDHGKVIDDGT
                     VARLGDDSYRWTSAEQHLRWFELNARGLDIEIDDVSETTAAIALQGPLARDVLGAATD
                     ADWSDLGYYRRRAGHAGKIQIDVSRTGYTGDLGYELWVKAEQATDLWDRLMEAGRPFA
                     LRPAGMLALDMARLEAGLILIEVDYTSATQALIPSQNYSPFEIGLGRLVNLNKESDFV
                     GRRALLAEQAVGGPMRRLVGLEIDWPSIEENFEQQGLPIHVPTAAWREQVPVYVGGRQ
                     VGRATSGTWSPTLKKHIALAQVDAAHEAVGSRLSIEQTVEGHRGRATATVAPLPFFDP
                     PRKRL"
     gene            complement(4333..4704)
                     /locus_tag="VMZ33_08220"
     CDS             complement(4333..4704)
                     /locus_tag="VMZ33_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17258.1"
                     /translation="MGQMDTYAGSARDSGTGPAREFDRRAAESQRLATDGADTHAPAT
                     NGAPTNGAPTNGHATHGAAVAQVLRAWRDVERQLAGMADDDPEAVTAKEQIARLRDEY
                     HRRVEEESQQRTNEADGTSRP"
     gene            complement(4832..>5065)
                     /locus_tag="VMZ33_08225"
     CDS             complement(4832..>5065)
                     /locus_tag="VMZ33_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR17259.1"
                     /translation="GRVVTRNEIIDAVWGPDFVSESNIVDRHVRSLRIKLQNDFRKPR
                     FIATVPGEGYRFMPTFSNSGWDSGQDEDTNQPH"
BASE COUNT          726 a         1724 c         1689 g          926 t
ORIGIN      
        1 gtgtgaatcc ggcacagtcc cgctacggtg accggcgctc aacctctggg tgagtgccgg
       61 aagtccggtc gccgggccca tggtcgtgcc gtatttcctt cgtgagaaag ggaacgcacc
      121 catgagccat gaccagaaaa gagccccttc cgcctcgcgc ggcaaggggt tttctcatgc
      181 ccgcgccggc acgccgggcg cctcctccac caggcatatg accccccagc gacctgcgca
      241 gagagacacg atgagccgcc ccacccgagt tttcctgctg agcctcgccc tgatcatcgc
      301 cgcatgctcg ggcgcaccac gcccgagcgc gacactgcat gtcgacccga cgatcccgac
      361 cgctttcccc ctgggtctgc gcgacgacga gggcacggaa gtcaccatca cggccgagcc
      421 gcagaccatc gtcagcctga cgccggcgaa tactgaaatc gtctacgccc tcggggccgg
      481 cgaccggctg cgcgggggca cggactttga cgacttccct gccgaggccg cggcattgcc
      541 cgacgtggtg gcgggcgtcc aggtgctgac cgagcagatc gttgcgatcg atcccgacct
      601 cgttctcgcc ggcggcaata acttcacgcc ggccgcggaa gtcgaacggc tgcgcggcct
      661 gggcgtcacc gtgctcgtgg tctaccccga gaccgtcgac gaggtcgtgg ccgacatcgg
      721 gctcatcggc cgcgcgattg gacgacatga cgaggccatg gcgatcacag aaggaatgga
      781 ggcccgcatc cgcgacatca ccagcgtggt ctcgcgtctg gacccactgc gcacgttcta
      841 cgagatcggc tacgggccgg acatctacgg gcctgcccca aactcgttca tcgcggacat
      901 ggtcgagctg gccggtggtg acgcgatcac gagcggtgat ccggctgtgt tcagcatttc
      961 gctcgaggat cttgtggaca aggatcccga ggtcatcgtc ctgggcgacg ctgcctacgg
     1021 gacgtgcccc gaaaccgtgc ccagccgtcc cggctgggac tcgatgacag cggtcgccga
     1081 aactgcgatc cggccggtag acgacatcgt tgtgacgagg ccggggccgc gactggccga
     1141 ggggctggca gcccttgcgc tggcaatcca ccccgacgcc cagatcacgc caccaaccgt
     1201 gggcgcggtg ctgtgcgcgg acccgggggt cccgtccaca ccgtgaacgc ggaggcaacc
     1261 gcccgacgag cggcggcgcg tcctgcctgg ctcgggttcc tgcgccatcg accggtggcg
     1321 ctggccgtgc tgggggtggg cgccctctgc gcgacgctca tcgcgggcgt gtggttgggg
     1381 agcgtcggca tcgctcccgg agacgtgctg gccatcctga cgcaccgcct cttccgttgg
     1441 ccggccgagc agacctggcc gtcgagcgcc gagacgatcg tcaccgaatt gcgactgccg
     1501 cgcgtgctca cggcgatggc cgtcggggcg gctctgtccg tcgccggtgc cacgtaccag
     1561 gggctgttgc gcaacccact cgccgatccc tacgtcctgg gcacggccag tggcgccgca
     1621 ctgggtgccg ccatcgcggt cctgatcccg atccagatcg gcctgctcgg actcggcatg
     1681 ctccatctct tcgcgttcgt cggcgcgctg ctggccgtgg cggtcgtcta tcgggtctcc
     1741 cggctcggcg cgctcaactc gctcacgtca gtgctgttga ccggctacgc catcgggtcg
     1801 ttgctggccg cagggctggc catggcgatg tacctctccg gcaacaacct gcgccagatc
     1861 ttctactacc tgctgggctc cttctcgctc gcgtcgtggc cgcagctggc cgtctcggcc
     1921 ctgctgatcg ctatcgggct cggtctcgtc ctgtcgcgcg cgcggtccct caacgccctg
     1981 ctgctgggcg aggaagcggc cgctcacctg ggtatcgacg ttcgccgtga tcgcctgatc
     2041 ctcctgggcg gcgcgaccct gatgacggcc gccgcggttg cggtaagcgg gctcatcggg
     2101 ttcgttgggc tggtgatacc gcacctcgtc cggctggtgg tcggacccaa cgcgcgcatg
     2161 ctcctcccgc tgtctgcgat ctacggcgcg gcgtttctcg ccctggccga cctgatcgcg
     2221 cgagcgcccg gtgaagttcc cgtgggcgtg gtcacggccc tgctcggggc gccgttcttc
     2281 ctctacctgt tgcgtcgcta tcgaaccgga tacgagctgt gagcggtttg gcgctcgacg
     2341 tccgcaacgc caccgtggct taccgcggtc gggtggtcgt cgaggacgtc agcttcgagg
     2401 ttggcgctgg cgagctggtg tgtctggtag gtccaaacgg cgccggcaag tcaacgctgt
     2461 tgcgcgccat ctcaggcacc gttgcgctca agagcggcat catcgccatc gatggggtgc
     2521 cgcgcgccga gcacgaccgt gtgtccctgg ctcggcgcct ggccgttgtc cccggccaga
     2581 cggtgattgg cttcccgatg cgcgtcgagg agcttgtcgc tctgggtcga atcccgcacg
     2641 agcacccgct gatgggtctg cgactcgcgg accgcgcggc catcgaccac gccatcgcgc
     2701 gcgtgggcat cgcgcacctg cgtggccgcg acgtgcgcga gctgtctctc ggggagcgtc
     2761 agctcgtggt gctggccatg accgtcgccc agggtgcgca gctgctgctg ctcgacgaac
     2821 cgaccgtcca tctggatctg cgacaccagg tcgaggtcat gcagcttctc gcagaccttg
     2881 cgcagcgcga cggagtaaca gttctgaccg tgctccacga cctctcgctg gccagccact
     2941 tcttcccgcg actgctgttg ctggacgaag gccggctcgt ggccgacggt cccgccggag
     3001 ccgtcctcac gccggaccaa gtgcgtgagg tctaccgcgt cgatccccga ttcgtgaccg
     3061 tggccacgtc aggttgagct agcggacgcg ctcagaggcg cttgcgtggc ggatcgaaga
     3121 agggcagtgg cgccaccgtt gccgtggcgc ggccgcggtg gccctccaca gtctgctcga
     3181 tagacagccg cgagcccacg gcctcgtgcg cggcgtcgac ctgtgccagc gcgatgtgct
     3241 tcttgagcgt cggactccat gtgccactgg tggcccgtcc aacttgtcgg ccgccgacgt
     3301 agaccggcac ctgctcgcgc caggcggccg tggggacgtg gatcggcagg ccctgttgtt
     3361 cgaagttctc ctcaatcgaa ggccaatcga tctcgagccc gaccagccga cgcatgggac
     3421 cgcccacggc ctgttctgca agcagcgccc gccgaccgac gaaatcggac tccttgttga
     3481 ggttgacgag tcggcccagc ccaatttcga acggtgaata gttctggctt gggattagtg
     3541 cctgcgtggc gctggtgtag tcgacctcga tcaggatcag gccggcctcg agtcgggcca
     3601 tgtccagggc cagcatgccc gccgggcgca gcgcgaaggg ccgacccgcc tccatcagcc
     3661 ggtcccacag atccgtggcc tgctccgcct tgacccacag ctcgtagccg aggtcgcccg
     3721 tgtagcccgt gcggctgacg tcgatctgaa tcttgccagc gtgtccggca cgacgccggt
     3781 agtagccgag gtcagaccag tctgcgtctg tggcggcgcc gaggacatcg cgcgccagcg
     3841 ggccttgcag ggcgatcgct gcggtggtct cgctgacgtc gtcgatctct atgtcgaggc
     3901 cgcgtgcgtt cagctcgaac caacgcagat gctgctcagc gctggtccag cggtatgagt
     3961 catcgcctag ccgcgcaact gtgccgtcgt cgatgacctt gccgtggtcg tcgcaccagg
     4021 gcgtgtagat gacctgcccc ggctggatct tggttgcgtc gcgagtgatg acgcggtcga
     4081 tgagtcgcgc cgcatccggg ccgcgcacga tgtacttgta gagcggcgac acgtcgatga
     4141 gcactgccgc gtggcggatc cccgagtact cgtggatgtg caggtggtcg taggcgctag
     4201 cggccaggta ccccgaccac tcccgccagc tctgcttgcg attgagcggc gcggttcgtt
     4261 catggaaggg agtcccggcg gtcacgcagg caatcctaag agagccagag cgaagagcca
     4321 ggggagcgtg gctcagggcc tggaggttcc gtccgcttcg ttggtccgtt gctgcgactc
     4381 ttcttcgacg cgccggtggt actcatcgcg aaggcgggcg atctgttcct ttgccgttac
     4441 tgcttcgggg tcgtcgtcag ccattccggc cagctgccgc tcgacatccc gccacgcacg
     4501 cagcacctgg gcgacagctg cgccatgggt cgcgtgaccg ttggtcggcg cgccgttggt
     4561 gggtgcgccg ttggtcgcgg gggcatgagt gtcggcgcca tcggttgcaa ggcgttggct
     4621 ttccgccgcc cgacggtcga actccctcgc tgggccagta cccgaatccc tggccgaacc
     4681 ggcgtaagta tccatctgcc ccatgacgcc accccaaaac ctgccagggc gcaatgcgcc
     4741 cgcctcttct taggggtgga tgttgtgggg ccgcgattgc aatgtcatta cggtccgtaa
     4801 tccgccgatg tgccccctcg ggaagggcgg atcagtgagg ctggttcgta tcctcgtcct
     4861 gaccggaatc ccatccactg ttggagaagg tgggcatgaa tcgatagccc tcccccggca
     4921 ccgtcgcaat gaatcggggc tttcgaaaat cgttctggag cttgatccgc aggctgcgga
     4981 catgccggtc gacgatgttg ctctcggaca cgaagtcggg gccccatacg gcgtcgatga
     5041 tctcgttcct cgtaacgaca cgtcc
//
LOCUS       DATLCO010000358         3110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8067648, whole genome shotgun sequence.
ACCESSION   DATLCO010000358 DATLCO010000000
VERSION     DATLCO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8067648"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..828)
                     /locus_tag="VMZ33_08230"
     CDS             complement(16..828)
                     /locus_tag="VMZ33_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUR17260.1"
                     /translation="MSDILQVERTGAGGAIVRVTLNRPEVHNAFNANLIGELRTTFLR
                     FADEPAEQLRVVVLRGAGPSFCAGADITWMRASLGLTREQNEQDAMVMAQMFDAIDRC
                     PVPVIARVQGASLGGGMGLCAVSDLVIAEAGARFGFTETRLGILPAVISPFVIAKIGE
                     THARALFPSGRRFDATRALRIGLVHEVVEGEGALDAAIDTALSDLLAAGPTAARAAKA
                     IVREVRGLPHESTRWHTARRIATQRTSGEGQEGLKAFLEKRDPAWNEAISDD"
     gene            complement(825..2108)
                     /locus_tag="VMZ33_08235"
     CDS             complement(825..2108)
                     /locus_tag="VMZ33_08235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR17261.1"
                     /translation="MEPNVGSTSGRVVQVSISRGGVPKLPIPVGRVGRLGVEGDAHHE
                     DTVHGGPHRALCLLAMEAIERMQADGHPIGPGSAGENLTTTGIEWSLLPVGTRARIGD
                     MLEIELSDSTTPCSTQVANFSDGNFNRMNIVVHPPDSRMYARVVSDGPVRPGDEIRLS
                     PPLNENAADELLLKRLDRAETKSSVAAWKAAKDAGFQIHVVEDGELAMSASPDIPGPA
                     FNQASGLARIPNLLSRATEFYDRQGTTGYVWLEAPPWPNAEVSLELGMFAGDPLAVPA
                     ESAPEGVLIRRIDPDEAERYTQVRSGSATAGGVTDAGPNPWPQVYAELARHNARQLFL
                     AEIDGRPVGNGSLHISARTGWLRGATVSPEARGRGIQRALVAARVAAAIEAGCDLVGA
                     AAETGAVSAQNLERMGFRQVGRRSSYVYEPQPRLL"
     gene            complement(2121..2552)
                     /locus_tag="VMZ33_08240"
     CDS             complement(2121..2552)
                     /locus_tag="VMZ33_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HUR17262.1"
                     /translation="MRFQRFGDRYQLRLESGDELMSTLTAFAERERIEYAAVSGLGAV
                     QSISLAYFVRDTKQYETYEFDEQFELLGATGNIVLRDGTPFPHVHASLGRRDLTVLGG
                     HVMRAVAWPTIELWVSREHQLVNRLPDDESGLALMDLPDRL"
     gene            complement(2563..>3110)
                     /locus_tag="VMZ33_08245"
     CDS             complement(2563..>3110)
                     /locus_tag="VMZ33_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR17263.1"
                     /translation="IQNWSALHLERGLGASPALGGAAPGVFAAFMFIGRAMGHPLGSR
                     VTERALLSGGAVVAAVGAAILAAAPSVLIALVGLAFSGAGISMVAPALFARAGRLAGP
                     GGRGAAIARVTSLGYSGFIFGPAVVGLIAQATDLRTALATLAVMALLVGIAGWLVMTG
                     GSAEKKGTFDDGEELLRTGRA"
BASE COUNT          521 a         1081 c         1023 g          485 t
ORIGIN      
        1 gtcaaacagt gcggctcaat cgtcggaaat agcttcattc catgccgggt cgcgcttctc
       61 gaggaaggcc ttgaggccct cctggccctc tcctgacgtg cgctgtgtcg cgatgcgccg
      121 ggctgtgtgc cagcgtgtcg actcgtgtgg aagtccgcgc acctcgcgca cgatcgcctt
      181 ggccgcccgc gcggctgtcg gcccggcggc cagaaggtca gacagcgctg tgtcgatcgc
      241 cgcgtcgagc gcaccttcgc cctcgaccac ctcatgtacc aggccgatgc gcagtgcgcg
      301 cgtggcgtcg aaccttctgc cggacgggaa caaggctcgc gcgtgggtct cgccgatctt
      361 cgcgatcacg aacggcgaga tgaccgccgg caggatgccc aggcgcgtct cggtgaagcc
      421 gaacctggcg ccggcctcgg cgattaccag atcgctcacc gcgcagagac ccatcccgcc
      481 gccgagtgac gctccctgga cgcgggcgat gacgggcacg ggacagcggt cgatggcgtc
      541 gaacatctgg gccatcacca tcgcgtcctg ttcgttctgc tcgcgtgtca ggccgagaga
      601 cgcgcgcatc cacgtgatgt ccgcgccggc gcagaaggac ggcccggcac cgcgcagaac
      661 gacgacgcgc agctgctccg ctggctcgtc ggcaaaccgc aggaaggtag tccggagctc
      721 cccgatgagg ttcgcgttga acgcattgtg aacctcgggt cggttgagcg tcacgcggac
      781 gatggcaccg ccagcgcccg tccgctcaac ctggagaatg tcgctcacag caacctcggc
      841 tgcggctcgt aaacgtagct cgatcggcgc cccacttgcc ggaaccccat gcgttccagg
      901 ttttgcgctg acacagcccc agtctctgcc gccgcaccca cgaggtcaca cccggcttca
      961 atggctgcgg ccacgcgtgc cgcgaccagg gcgcgttgaa tgccgcggcc acgggcctcc
     1021 ggggacaccg tggcgccgcg cagccagcca gttctggcag atatgtgcag cgagccgttt
     1081 ccgacggggc gcccgtcgat ctcagcgagg aacagctgcc gcgcgttgtg gcgggcaagc
     1141 tcggcgtaaa cctgcggcca cggattcgga ccagcgtccg tgacgccgcc cgcagttgcg
     1201 ctccccgacc gaacctgtgt gtagcgttcg gcctcgtcag gatcaatgcg cctgatcagc
     1261 acgccctcag gagccgattc ggcggggacc gccagcgggt cgcccgcgaa catgcccagc
     1321 tccagcgaaa cctcagcatt cggccacggt ggcgcctcca gccagacgta tccagtcgtg
     1381 ccctgccggt cgtagaactc ggtcgcgcgg gaaaggaggt tggggatgcg ggccaaacca
     1441 gatgcctgat tgaaggccgg cccggggatg tcgggcgacg cggacattgc caattcgccg
     1501 tcctcgacca cgtggatctg gaagccggca tccttggcag ccttccacgc cgcgacgctc
     1561 gatttggtct cagcgcgatc gagacgcttc agcagtaatt cgtctgctgc attttcgttc
     1621 aggggtgggc tgagccggat ctcgtcaccc ggccgcacgg ggccgtccga cacgactcgg
     1681 gcgtacatgc ggctgtccgg gggatgcaca acgatgttca tccggttgaa gttgccatcg
     1741 ctgaagttcg cgacctgggt ggagcatggt gtggtcgagt ccgacagctc gatctccagc
     1801 atgtcgccga ttcgggcgcg cgtgcccacc ggcagcagcg accactcgat ccccgtcgtc
     1861 gtcaggttct cgccggcgct ccccggcccg atggggtgac cgtcggcctg catccgctcg
     1921 attgcttcca tcgccagcag gcaaagcgcc cggtgaggcc cgccgtgaac cgtgtcctcg
     1981 tgatgagcgt cgccctcgac gccgaggcgg ccaactcgac ccaccggaat tgggagcttg
     2041 ggcacgccgc cgcgcgagat gctgacctgt acgacacgac ctgaggttga gccgacattg
     2101 ggttccatcg gccgccgatg ctatagccgg tcgggcaggt ccatgagggc cagcccgctc
     2161 tcgtcgtcgg gcaggcgatt cacgagctga tgctcgcggc tgacccagag ctcgatggtc
     2221 ggccacgcga cggcgcgcat cacgtggccg ccgagtacgg tcaggtcacg tcggcccagc
     2281 gacgcgtgga cgtgcggaaa cggcgtcccg tcgcgcaaaa cgatgttgcc ggtcgcgcca
     2341 agtagctcga attgctcgtc aaactcgtag gtctcgtact gctttgtgtc gcggacaaaa
     2401 tacgccagcg agatggactg gaccgcgcca agcccggaca cagccgcgta ctcgatcctc
     2461 tcccgttcag cgaaggcagt cagggtcgac atcagctcgt cgcccgactc gagccgcagc
     2521 tggtaccggt cgccaaaccg ctgaaacctc acccgtccga tcctacgcgc gacctgtccg
     2581 gagcagctcc tcgccgtcat cgaacgtgcc cttcttttcg gcgctgccac cagtcatcac
     2641 cagccagccg gcgatgccga ctaagagggc catgacggcc agcgtggcaa gcgccgtacg
     2701 gaggtcggtc gcctgggcga tcagcccgac tactgccggg ccaaagatga agccgctgta
     2761 gcccagcgac gtgacacgcg cgatggcagc tccacggccc ccgggtccgg cgagccgacc
     2821 cgcgcgggcg aagagcgccg gcgcgaccat gctgatgccg gcgccgctaa aagccagacc
     2881 gaccagggca atgagcaccg acggcgcggc cgccaggatt gcggcgccaa cggcggccac
     2941 gaccgcgccg cccgacagga gtgcccgctc ggtgacgcgc gagcccagcg gatggcccat
     3001 agcgcggccg atgaacatga acgcggcaaa gacaccgggc gctgcgccgc cgagcgcggg
     3061 actcgcgcca aggccacgtt cgagatgcag cgcgctccag ttctggattg
//
LOCUS       DATLCO010000359         3808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10304313, whole genome shotgun sequence.
ACCESSION   DATLCO010000359 DATLCO010000000
VERSION     DATLCO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10304313"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /gene="hutU"
                     /locus_tag="VMZ33_08250"
     CDS             <1..1201
                     /gene="hutU"
                     /locus_tag="VMZ33_08250"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HUR17264.1"
                     /translation="GGTLRGRIVLTAGLGGMGGAQPLAVTMNEGVALVIEVNESTARR
                     RMDQGFVDRITYDLDEGLAWARAAAAEGRAQSIALVGNAAVIEPELVRRGERFDVVTD
                     QTSAHDALNGYIPAGLDFRDASALRDRDPQEYIAAAMESMATQVRAMLALQGAGAVTF
                     DYGNNIRAQALAAGVANAFDFQGFVPAFIRPLFAEGKGPFRWAALSGDPADIHRTDEA
                     VLELFPEDEGLRRWIRMAQEKVPFQGLPARICWLGYGERARAGARFNELVASGEVSAP
                     LVIGRDHLDSGSVASPNRETEGMRDGSDAIADWPLLNALVNTAAGASWVSIHHGGGVG
                     IGYSQHAGMVVVADGTPLAAQKLERVLTTDPGMGVIRHADAGYERAIEVAHERGVRIP
                     MLDHTPD"
     gene            complement(1288..2046)
                     /locus_tag="VMZ33_08255"
     CDS             complement(1288..2046)
                     /locus_tag="VMZ33_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17265.1"
                     /translation="MADEIRDLVRDINRQQRAGFEERLRRELISKDRAWLVDQLVALA
                     LADRERRLGTAHDSESDATEEPAERTERAKRVRELRLNTAKVRAFAATHEGVERARLI
                     ASGHLAAEAPGKGTDLLTSAHRGPSGDQLLDEAKDMLFGLLFGDDSTDTRLDRVQQEL
                     LTIALPRGKANALDFMRASTELSAAGTWQDPDSVSNDARAENVLIEVEFGETADEVVG
                     AGIVAALSVINNLEINEQVLYARMINVEESTLIS"
     gene            complement(2067..3392)
                     /locus_tag="VMZ33_08260"
     CDS             complement(2067..3392)
                     /locus_tag="VMZ33_08260"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR17266.1"
                     /translation="MTSASRSPLAVRDFRLLWLGESVSVLGDQFALVALPWLALLLTG
                     SPLALGTVLALMAVPRAVLMVVGGAYVDRLSPRTVMLVSNAVRFVAVAALGAIVLAGA
                     AQLWMLYIFALVFGIADAFFYPAHTAIVPDLVSGEQLQRANGIAQGTAQLSVLVGPAV
                     AGIAIASLSVSQLGPGLTGVGVALLIDALTFLVSLLALALIKPRLAHPGATGTSVMTQ
                     IREGIAFVWQTKALRVVMLLSMFLNLLITGPFEIGLPVLAFSRLPEGAAAFGLMTAAF
                     GGGSVLGLLAATLLPALPPGRFGTVLLLLIALVGLAIVAMAFATSLIAAIAVAAVIGA
                     ILGYTNVSFMTWIQRRIPRALMGRVMSLLMFSSIALVPISMAVAGALVEISLEGLFIV
                     AGVGMALITLLAMLSRSVRQMGLEPVIDDEHEPGTSEAQATSPPVPAAV"
     gene            complement(3433..3621)
                     /locus_tag="VMZ33_08265"
     CDS             complement(3433..3621)
                     /locus_tag="VMZ33_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17267.1"
                     /translation="MFVVTTDLDEPYVPFVTGRHSSGRMHEVRRRESRAGTPRRLVGK
                     ALIRAGLAVAGLDGSSSR"
BASE COUNT          652 a         1242 c         1317 g          597 t
ORIGIN      
        1 cgggggcacc ctgcgcggac ggatcgtcct gacagcgggc ctcggcggca tgggtggcgc
       61 ccagccactg gcagtcacga tgaacgaggg cgttgcgctc gtaattgagg tcaacgagtc
      121 gacggcacgg cggcgaatgg atcagggctt tgttgatcgg attacgtacg acctcgacga
      181 agggctggcc tgggcgcgcg ctgcagcggc cgagggtcgc gcgcagtcga tcgccctggt
      241 cggcaatgcg gcggtgatcg agcctgagct tgtccgccgc ggcgagcggt tcgacgtggt
      301 cacggaccag acctccgccc atgacgcgct caacggttac atcccggctg gcctcgactt
      361 tcgcgacgcc tccgcgctgc gagaccgcga cccgcaggag tacatcgcgg cggcgatgga
      421 gtcgatggca acgcaggtgc gagcgatgct cgcgctccag ggagccggtg cggtcacgtt
      481 cgactacggc aacaacatcc gcgcgcaggc tctggcggcg ggcgtggcca acgcttttga
      541 tttccaaggg ttcgtcccgg cgttcatcag gccgctcttc gccgagggca aggggccgtt
      601 ccgctgggcg gcgctgtctg gcgacccagc cgacatccac cgcaccgacg aggccgtcct
      661 cgagctcttc cccgaggacg agggtttgcg gcgctggatt cgcatggccc aggagaaggt
      721 tccgttccag ggtttgccgg cccggatctg ctggctcggt tacggcgagc gggcacgtgc
      781 cggggcgcgc tttaacgagc tcgtcgcatc aggggaagtg tccgcacccc tcgtgatcgg
      841 ccgcgaccac ctcgattccg gctcggtggc ctcgcccaac cgcgagacgg agggcatgcg
      901 cgacggctcg gacgccatcg ccgactggcc tctgctcaat gcgctggtca acaccgccgc
      961 cggtgccagc tgggtctcga tccaccacgg tggcggcgtt ggcatcggct actcgcagca
     1021 cgcggggatg gtcgtcgtcg cagacggcac gccgctcgca gcgcaaaagc tcgagcgggt
     1081 tctaacgact gacccgggca tgggcgtcat ccgtcacgcc gatgccggct acgaacgggc
     1141 catcgaggtg gcacacgagc gcggggtacg aatcccgatg ctcgatcaca ccccggacta
     1201 gggtcgcctc cgccgccagc gccttggccg tcgtcgccct gctgtcacgg gctgtgccgc
     1261 gatcgattca gtgatggtcc gtgcaggcta ggagatgagc gtcgactcct cgacgttgat
     1321 catccgcgca tagagcacct gctcgttgat ctccaggttg ttgatcaccg acagtgccgc
     1381 cacgatcccg gcgcccacga cctcgtcggc ggtctcgccg aactcgacct caatcagcac
     1441 gttctcggca cgcgcgtcgt tcgaaacgct atcggggtcc tgccacgtgc cggctgccga
     1501 cagctcggtt gaggcacgca tgaagtcgag agcgttggcc ttgccacgtg gcagcgcaat
     1561 ggtgagcagc tcctgctgga cgcgatcgag gcgcgtgtca gtcgagtcgt cgccgaaaag
     1621 caggccgaac agcatgtcct tggcttcgtc gaggagctga tcgccagacg gaccacgatg
     1681 ggctgacgtg agcaggtccg tgcctttccc tggcgcctca gctgcgagat gaccgctggc
     1741 tatcaatcgg gcgcgctcga cgccctcgtg cgtggccgcg aacgcgcgca ccttcgccgt
     1801 attcagtcga agttcgcgca cacgtttcgc gcgctccgtg cgttcggctg gctcctcggt
     1861 cgcatcggac tcggagtcat gagccgtgcc cagccgtcgc tcgcggtcgg ccagtgcaag
     1921 cgcgaccagc tgatcgacca gccacgcccg atctttgctt atcagttccc gacgcagccg
     1981 ttcctcaaag ccagcccttt gctggcggtt gatgtcgcgt acgagatcgc ggatttcatc
     2041 ggccacgacc agagcctagc ggcgtgtcaa acggctgcgg gaacgggcgg gctcgtcgct
     2101 tgggcttctg acgtcccggg ctcgtgctcg tcgtcgatga cgggctccag gcccatctgt
     2161 cgaacgctgc gtgacagcat ggcgagaagc gtgatgagcg ccatcccgac ccccgcgacg
     2221 atgaacaacc cttccaggct gatctcaacc agcgcaccgg cgacggccat cgagatcggc
     2281 acgagtgcga tcgaggagaa catcaggagg ctcatcacac ggcccatcag cgcgcgcggg
     2341 atgcgccgct ggatccacgt catgaatgag acgttcgtgt agccgaggat cgcgccgatg
     2401 accgcggcga cggcgatcgc agcgatgagg gaggtcgcga aggccatggc tacgatggca
     2461 agcccaacca gggcgatgag cagcagcaag acggtcccga atcggcccgg tggcagcgcc
     2521 ggcagcaacg tcgcggcgag caggcccaga acagagccac cgccgaatgc cgcggtcatc
     2581 aatccgaacg ccgcggcgcc ctcggggagg cgcgagaagg caaggaccgg aagaccgatt
     2641 tcgaacgggc ccgtgatcaa gaggtttagg aacatgctga gcagcatcac cacgcgcagt
     2701 gccttggtct gccagacgaa tgcgatccct tcgcggatct gcgtcatcac ggaggtcccg
     2761 gtggcgccgg gatgcgcaag gcgcggcttg atgagcgcca acgccaggag cgagacgagg
     2821 aacgtcaggg cgtcgatcag cagggcaacg ccaacgcccg tcaggcccgg tccgagctgg
     2881 ctgacgctca ggctcgcgat cgcgataccg gcaacagccg gcccgacgag caccgacagc
     2941 tgtgccgttc cctgggcgat gccgttggcc cgctgaagct gctcaccgct gacgaggtcg
     3001 gggacgatcg cggtgtgtgc ggggtagaag aaagcatcgg cgatgccgaa tacgagggca
     3061 aagatgtaga gcatccagag ctgcgcggcg cccgccagca ctatcgcacc gagggcggcc
     3121 acagcgacga accgcacggc gttggagacg agcatcaccg tccgcgggga gaggcggtcc
     3181 acgtacgcgc cgccgaccac catcagcacg gcacgcggca ctgccatcag cgccagcacg
     3241 gtgccgagcg cgagcgggct gccggtcagt agaagcgcga gccacggcag ggcgacgagg
     3301 gcaaattggt cgccgagcac cgagacggac tcgcccagcc acagcagccg aaagtcgcgc
     3361 acggcgagcg gcgaacggga cgcggaggtc atggtgatgc ctggggcctg ggcttccctg
     3421 ggaccgtcgc tgttagcggc tggaggagcc gtcgagaccg gcaactgcca gcccggctcg
     3481 gatgagagcc ttgccgacca agcggcgcgg cgttccggcg cgagactcgc ggcggcgcac
     3541 ttcgtgcatc cgccctgatg agtgccgacc ggtgacgaac gggacgtagg gctcgtccag
     3601 gtcggtggtg actacgaaca tctgtggtgt cctttcttgt gactttgggc ttgctattcg
     3661 ctgacgatca ggacgcgggt cgcgccgtcc tcgtcgtcga cttcgaggat tggcccgcgt
     3721 gagccggcat cgatcgcggt gcggatgcgt gctgcgtttt catcggacag gccgggcagc
     3781 aggtgactcg cccacgcggc gacgttga
//
LOCUS       DATLCO010000360         2867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8075981, whole genome shotgun sequence.
ACCESSION   DATLCO010000360 DATLCO010000000
VERSION     DATLCO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8075981"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(417..1121)
                     /locus_tag="VMZ33_08270"
     CDS             complement(417..1121)
                     /locus_tag="VMZ33_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HUR17268.1"
                     /translation="MIGITRSPRFSLADVALYLVGVAGLAMCLTLIWLSMRAVMNVGG
                     FCAEGGPYVIETRCPAGTPLAITFGVFGLFVFGGLAVWKGVAIGGRYAGLVALAWPAL
                     FISLGWNFLEFAFNPPGPPGQGIVWGWLIPGVVFIVMGAGPLLLALDIGKEGLVGSAR
                     TRAANRIAPDDRYALRANRQQLVNDLRAAKENAEDAGDLVTKLERLSALRDAGDLTSR
                     EFEDAKRAILRETRAT"
     gene            1225..1482
                     /locus_tag="VMZ33_08275"
     CDS             1225..1482
                     /locus_tag="VMZ33_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17269.1"
                     /translation="MAIMPRPFGTSTSATDVPSGGPTAPLRRCTFRRVSTIAQARALP
                     VYEVACTFPDRKRTVALGDIEAARPICASCTFQGIFRADSD"
     gene            1521..1739
                     /locus_tag="VMZ33_08280"
     CDS             1521..1739
                     /locus_tag="VMZ33_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17270.1"
                     /translation="MTFPGNEGVNLGDLLARSGMAQPSAAEATRLTPDRRHRQAGSWP
                     KGQTIVRIGLVAIVAILVIGWILSALNS"
     gene            complement(1756..2111)
                     /gene="rnpB"
                     /locus_tag="VMZ33_08285"
     ncRNA           complement(1756..2111)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VMZ33_08285"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2167..2727)
                     /locus_tag="VMZ33_08290"
     CDS             complement(2167..2727)
                     /locus_tag="VMZ33_08290"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HUR17271.1"
                     /translation="MSTRARAASTSPRRSWKCRAPSSIALPLARRRSHALPERLLIRA
                     DGAARGNPGPASAGAVLIDASIAGAGRPDAPVVARIARPLGIQTNNYAEYTGVILALE
                     KALALGAREVELVLDSLLIVEQLSGRWKVKHPAIRPLAARAMELLRQFERWSLRHERR
                     ENNIQADALANLALDDPAAARAAEHR"
     gene            complement(2615..>2867)
                     /locus_tag="VMZ33_08295"
     CDS             complement(2615..>2867)
                     /locus_tag="VMZ33_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17272.1"
                     /translation="RPEYVGPAAIVNAHRFIFDSRDDHAGERLEILADQDGVWRCRTI
                     FNCVDACPRGINITQAILEVSSAIVDRPSTRPQKVARTA"
BASE COUNT          463 a          994 c          922 g          488 t
ORIGIN      
        1 gggacggcaa cgcgcccctt ggcgtcgatc gcgtggcggt actcgccggt gaacacacga
       61 ctacctctgc tcggcctgtg ccgggtcgcg gggaagacgg ttgggcgttc caccgcgggg
      121 tcgtgcccta cccctaggcg attgccctac cgtcctctct ccccactttg atccactccg
      181 gaccacttcg tggcatcata cgcgcccatt ccgggtcgtc aagtaccaaa cttatgacgg
      241 gttccccacc cggttcggag aggctgcgtt cagttacgga cgagcgccaa cggccgaagg
      301 tcagccgatc gagttgaaga accacactcc cgttgctagc ccaaggacga tcgcggcaac
      361 gaccaggagc aggagcacgc gcgtgagccg accagagtcc gtgccggcgg ggcagctcac
      421 gtggcgcggg tctcgcgcag gatggctcgc ttggcgtcct cgaactcgcg gcttgtcagg
      481 tcgccggcgt cacgcagcgc ggacagccgc tcgagcttcg tcacgaggtc gcctgcgtcc
      541 tcggcgtttt cctttgccgc ccgcaggtcg ttcaccagct gctggcggtt ggcccgcagt
      601 gcgtatcgat cgtccggcgc aattcggttg gcggccctgg tccgggcgct cccgacgagg
      661 ccctctttgc cgatgtccag cgccagcagc agcggtcccg cgcccatcac aatgaacacg
      721 actccaggga tgagccagcc ccacacgatc ccctgccccg gcggcccggg cgggttgaat
      781 gcgaactcca ggaagttcca gccaaggctg atgaatagtg ccggccacgc gagtgccacc
      841 aatccggcgt accggccgcc aatcgcgacg cccttccaca ccgccagccc gccgaacacg
      901 aacagcccga agaccccgaa cgtgatcgcc agcggcgttc ctgccgggca ccgtgtttcg
      961 atcacgtacg gcccaccctc ggcgcagaaa ccgccgacat tcatcacggc ccgcatcgac
     1021 aaccagataa gggtgagaca catcgccagg ccggcgaccc ccacgagata gagggccacg
     1081 tcggccagcg agaaccgtgg ggatctggtg atgccgatca cgctcgcgat ggtagtcagc
     1141 gcagcggcgg cggacaggcc aactagtacg cctagtactg ctggcagcct tgtcgcacga
     1201 cccgttcgag cctaggtttc aggcatggca atcatgccgc ggccgttcgg cacgtccaca
     1261 tccgcgaccg atgttccgtc gggcggcccg accgcacctc ttcggcgctg caccttccgg
     1321 cgcgtcagca cgatcgcgca agcgcgagcg ctgccggtgt acgaggtcgc gtgcacgttc
     1381 cccgatcgga agcgcaccgt ggccctcggt gacatcgaag cggcgcgtcc gatctgcgct
     1441 tcgtgcacct tccagggaat tttccgggcc gactcagact agaccgccaa gcgcatactc
     1501 attgccggag gttcagtgca gtgactttcc ccggcaatga aggcgtaaac ctgggtgacc
     1561 tgctcgcgcg atccggtatg gcgcagccga gcgctgcaga ggcgacgcgc cttacgcctg
     1621 atcgtcgtca ccggcaagcg ggctcgtggc cgaaaggcca gaccatcgtt cgcatcgggc
     1681 tcgtcgcgat cgtggccatc cttgttatcg gctggatcct gagcgcgctg aattcctagc
     1741 cgctcaaaga gattgccagc gagacggtct gtaagccgag ttctgtcccg acccttgcgg
     1801 atcggggacg gtcatccatc tacgacggcg gttgcccgac gtctcaagcg gccgacccga
     1861 gagcgaagcg acgccgctcc cgccgggctt tcgcccgact gctctcctat ttggccttgc
     1921 tccaggtaga gcttgcccgt ttcacccggc ccagccgacc ttgcggcccg cctgccggct
     1981 cgtctctgtg gcgctggtcc tcgccttccg gcggacggga gttacccgct accctgcgtc
     2041 gcggagctcg gactttcctc gtatcagtaa cggctttcgc cgacgggata cgcgaccgtc
     2101 cgaccatctc gctggcccgt caattctacc ttggaacatc cgttcgacca agaggtcggc
     2161 cgcgattcag cggtgttcgg ccgctcgggc ggctgccgga tcgtcgaggg ccagattggc
     2221 tagggcgtcg gcctgaatgt tgttctcgcg gcgctcgtgg cggagcgacc agcgctcgaa
     2281 ctggcgcagg agttccatgg cgcgcgccgc cagcgggcgg atcgcgggat gctttacctt
     2341 ccagcggccc gacagctgct cgacgatgag cagcgagtcg agcacaagct caacttcgcg
     2401 cgcgcccagc gcgagcgcct tctcgagcgc gaggatcacg cccgtgtact cggcatagtt
     2461 gttcgtctgg attcccagag gtcgtgcgat gcgggccact acgggcgcgt cgggtctgcc
     2521 cgcgccggcg atcgaagcat cgatcagcac ggcgcctgcc gacgcggggc cggggttgcc
     2581 acgggccgca ccatcggcgc ggatcagcag tcgctcaggc agtgcgtgcg accttctgcg
     2641 ggcgagtgga agggcgatcg acgatggcgc tcgacacttc caggatcgcc tgggtgatgt
     2701 tgatgccgcg cgggcacgcg tcgacacagt tgaagatcgt ccggcaacgc cacacgccgt
     2761 cctggtcggc caggatctcc agccgctccc cggcgtggtc gtcgcgcgag tcgaagatga
     2821 agcggtgcgc gttgacgatc gccgccgggc cgacgtactc aggccgc
//
LOCUS       DATLCO010000361         3033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_8076011, whole genome shotgun sequence.
ACCESSION   DATLCO010000361 DATLCO010000000
VERSION     DATLCO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8076011"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(596..1300)
                     /locus_tag="VMZ33_08300"
     CDS             complement(596..1300)
                     /locus_tag="VMZ33_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17273.1"
                     /translation="MIGITGSRRFSLADVALYLVGVAGLAMCLTLVWLSMRAVMNVGG
                     FCAEGGPYVIETPCPAGTPLAITIGIFGLFVFGGLVVWKGAAIGGRYAGLVALAWPAL
                     FISLGWNFLEYAFNPPGPPGQGIVWGWLIPGVVFIVMGAGPLLLALDIGKEGLVGSAR
                     TRAANRIAPDDRYAPRANRQKLVNDLQAAKENAEDAADLVTKLERLSALREAGDLTSG
                     EFEDAKRAILRQVRAT"
     gene            1404..1661
                     /locus_tag="VMZ33_08305"
     CDS             1404..1661
                     /locus_tag="VMZ33_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17274.1"
                     /translation="MAIMPRPFGTSTSATDIPSAGLTAPLRRCTFRRVSTIAQARALP
                     VYEVACTFPDRKRAVALGDIETARPICASCTFQGIFRADSD"
     gene            1701..1919
                     /locus_tag="VMZ33_08310"
     CDS             1701..1919
                     /locus_tag="VMZ33_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17275.1"
                     /translation="MTFPGNEGVNLGDLLARSGMAQPNAADATRLTPDRRHRQPGSWP
                     KGQSIVRIVLVAIVAILVSGWILSALNS"
     gene            complement(1941..2296)
                     /gene="rnpB"
                     /locus_tag="VMZ33_08315"
     ncRNA           complement(1941..2296)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VMZ33_08315"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2352..2843)
                     /locus_tag="VMZ33_08320"
     CDS             complement(2352..2843)
                     /locus_tag="VMZ33_08320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HUR17276.1"
                     /translation="MGLPLARRRSHALPERLLIRADGAARGNPGPSSAGAVLIDASLA
                     GASRPDAPVVARISRPLGIQTNNYAEYTGVILALEKALALGAREVELVLDSMLIVEQL
                     SGRWKVKHPAIRPLAARAMELLRQFDRWSLRHERRENNIQADALANLALDDPAAARAA
                     EDR"
     gene            complement(2800..>3033)
                     /locus_tag="VMZ33_08325"
     CDS             complement(2800..>3033)
                     /locus_tag="VMZ33_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17277.1"
                     /translation="PAAIVNAHRFIFDSRDDHAGERLEILADQDGVWRCRTIFNCVDA
                     CPRGINITQAILEVSSAIVDRPSARPQKVARTA"
BASE COUNT          510 a         1036 c          965 g          522 t
ORIGIN      
        1 gggacggcaa cgcgcccctt ggcgtcgatc gcgtggcggt actcgccggt gaacacacga
       61 ctacctctgc tcggcctgtg ccgggtcgcg gggaagacgg ttgggcgttc caccgcgggg
      121 tcgtgcccta cccctaggcg agtgccctac cgtcctctct ccccactttg atccactccg
      181 gaccacttcg tggcatcata cgcgccgatt ccgggtcgtc aagtaccaaa ctgatgacgg
      241 gttccccacc ggtctcggaa ggcgagaagc tgggtgcggt ggcacctggc gagcgtcggt
      301 accgccgatt tgggacgtca ggtatgcatc acatgattga cggcgtacgg gaaacgccac
      361 ggagtgacac ctgattagtg atgggcggcg agccggctcg ctatcggcct ttggcacgcg
      421 ctgacgcagt catgtcctgc atatgtgttc acttagggac gagcgtcagc ggtcgcgggt
      481 cagccgatcg agttgaagaa ccaaactccc gctgctaccc caaggatgat cgcggcaagg
      541 accaggaaca ccatcacgcg cggtggacga tcagagtcag ctccggcggg gaggctcacg
      601 tcgcacggac ttggcgcagg atggcccgct tggcgtcctc gaactcgccg ctcgtcaggt
      661 cgccggcctc tcgcagcgcg gatagccgct cgagcttcgt caccaggtcg gctgcgtcct
      721 cggcgttttc ctttgccgcc tgcaggtcgt tcaccagctt ctggcggttg gcccgcggtg
      781 catatcgatc gtccggcgca attcggttgg cggccctggt ccgggcactc ccgacgaggc
      841 cctccttgcc gatgtccagc gccagcagca gcggtcccgc gcccatcacg atgaacacga
      901 ctccagggat gagccagccc cacacgatcc cctgccccgg cggcccaggc gggttgaatg
      961 cgtactccag gaagttccag ccaagactga tgaatagtgc cggccacgcg agtgccacca
     1021 gcccggcgta ccggccgcca atcgcggccc ccttccagac caccagcccg ccgaacacga
     1081 acagcccgaa gatcccgatc gtgattgcca gcggcgttcc tgcggggcac ggtgtttcga
     1141 ttacgtacgg cccaccctcg gcgcagaaac cgccgacatt catcacggct cgcatcgaca
     1201 accagacaag ggtcaggcac atcgccaggc cggcgacgcc cacgagatag agggccacgt
     1261 cggccagcga gaaccgtcgg gatccggtga tgccgatcac gctcgcgatg gtagtcagcg
     1321 cggcggaggc ggacaggcca actagtacgc ctagtactgc tggcggcctt gtcgcacgac
     1381 ccgttcgagc ctaggtttca ggcatggcaa tcatgccgcg gccgttcggc acgtccacat
     1441 ccgcgaccga tattccatcg gccggcctga ccgcacctct tcggcgctgc accttccggc
     1501 gcgtcagcac gatcgcgcaa gcgcgagcgc tgccggtcta cgaggtcgcg tgcaccttcc
     1561 ccgatcggaa gcgggccgtg gccctgggcg acatcgagac ggctcgtccg atctgcgctt
     1621 cgtgcacctt ccagggaatt ttccgggccg actcagacta gaccgctaaa gcgcatactc
     1681 attgccggag gttcagtgca gtgacgttcc ccggcaatga aggcgtaaac ctgggtgacc
     1741 tgctcgcgcg atccggtatg gcgcagccga acgcagcaga cgcgacgcgc cttacccctg
     1801 atcgtcgtca ccgccaaccg ggctcgtggc cgaaaggcca gagcatcgtt cgcatcgtgc
     1861 tcgtcgcgat cgtggccatc cttgtcagcg gctggatcct gagcgcgctc aattcctaag
     1921 cgctcaatca agaagaatta ccagcgagac ggtctgtaag ccgagttctg tcccgaccct
     1981 cgcggatcgg ggacggtcat ccatctacga cggcggttgc ccgacgtctc aagcggccga
     2041 cccgagagcg aagcgacgcc gcccccgccg agccgaagcc cgactgctct cctatttggc
     2101 cttgctccag gtagagcttg cccgtttcac ccagcccagc ggacctttcg gtctgcctgc
     2161 tgactcgtct ctgtggcgct ggtcctcgcc tttcggcgga cgggagttac ccgctaccct
     2221 gcgtcgcgga gctcggactt tcctcgtatc agtaacggct ttcgccgacg ggatacgcga
     2281 ccgtccgacc atctcgctgg ccccccaatt ctaccttgga agatccgctc gaccaagggg
     2341 tcgacccggg ttcagcggtc ttcagccgct cgggcagctg ccggatcgtc cagggcgagg
     2401 ttcgccagcg cgtcggcttg aatgttgttc tcgcggcgct cgtggcggag cgaccagcga
     2461 tcgaactggc gcaggagttc catggcgcgc gccgccagcg ggcggatcgc ggggtgcttc
     2521 accttccaac ggcccgacag ctgctcgacg atgagcatcg agtcgagcac gagctccact
     2581 tcgcgcgcgc ccagtgcgag cgccttctcg agcgccagga tcacgccagt gtactcggcg
     2641 tagttattcg tctggatgcc gaggggccgc gatatccggg cgaccaccgg cgcatcagga
     2701 cggctggcac cggcaagcga tgcgtcgatc aacacggccc cggcggagga tggcccaggg
     2761 ttgccacggg ccgcgccatc ggcgcggatc agcagtcgct caggcagtgc gtgcgacctt
     2821 ctgcgggcga gcggaaggcc gatcgacgat ggcgctcgac acttccagga tcgcctgggt
     2881 gatgttgatg ccgcgcgggc acgcgtcgac gcagttgaag atcgtccggc aacgccacac
     2941 gccgtcctgg tcggccagga tctccagccg ctccccggcg tggtcgtcgc gcgagtcgaa
     3001 gatgaagcgg tgcgcgttga cgatcgccgc cgg
//
LOCUS       DATLCO010000362         4156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10326932, whole genome shotgun sequence.
ACCESSION   DATLCO010000362 DATLCO010000000
VERSION     DATLCO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10326932"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VMZ33_08330"
     CDS             complement(<1..290)
                     /locus_tag="VMZ33_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR17278.1"
                     /translation="MPDAADVVIIGGGPAGAATAIRLALRGISAIVVDYAAEPRWRAC
                     GVFSSPLSRRHLADLGLGAATIKSLYRPISALNLESTRGVRCRIEYERGHACG"
     gene            complement(296..982)
                     /locus_tag="VMZ33_08335"
     CDS             complement(296..982)
                     /locus_tag="VMZ33_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUR17279.1"
                     /translation="MRRSTKAEYLDGPLDTVILSGNLRDLARVNRWLGGANLSWRAIR
                     PFIEIGRTTRIVDVGTGGGDIPHMLLRRDKHRHQLHVLATDVRAELVDVARATAAAGP
                     SFEVRQDPPDRIDSPDGSVDVAHCSLVLHHLDADQAVALLLEMRRVATGAVVINDLAP
                     GWHWWIGAWALTRALTRNVYTRHDAPLSVRRAYSVDEVRSLAEQAGLRLVARYDALPA
                     YRYALVFVRA"
     gene            1081..2649
                     /locus_tag="VMZ33_08340"
     CDS             1081..2649
                     /locus_tag="VMZ33_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUR17280.1"
                     /translation="MADRRRRTSTTATRALAPRTRSNNVGRGTDSPSAWGLGDGQAVP
                     WDYAPAPESRDVVQLKSRYGLFIGGREVAPRSGQWFTTIDPATEEPLAEIARATVEDV
                     NQAVSVARRAFKRGWGDLPGSERAKYLFRIARILQERSREFAVLESLDGGKPIKESRD
                     VDVPLAAAHFWYYAGWADKLEYAFPNTEPRPLGVAAQVIPWNFPLLMLAWKIAPALAA
                     GNTVVLKPASSTPLSALFFADVVRQAELPAGVVNIITGPGEIGLALVKHPDVDKVAFT
                     GSTMVGKEIGRAIAGSDKALTLELGGKAANIVFDDAPIDQAIEGVVNGIFFNQGEVCC
                     AGSRLFVQESIAEPFIERLKDRLSTLRVGDPLDKNTDVGAINNSAQLAKIEELIEAGQ
                     AEGADMYQPACDLPTRGYFVRPTLFTNVAQSSRIAQEEIFGPVLSVLTFRTPAEAIEK
                     ANNTAYGLSAGVWTDKGSRILSMVSQLRAGVVWANTFNRFDPTSPFGGYKESGFGREG
                     GLHGLLSYMDTGDH"
     gene            2649..3587
                     /locus_tag="VMZ33_08345"
     CDS             2649..3587
                     /locus_tag="VMZ33_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUR17281.1"
                     /translation="MAATRQRRPAATEAVVTPKQARIPVRKTYKLYVGGQFPRSESGR
                     SYLAKAPDGTPLANAVRSSRKDVRDAVRTARGAAGGWASKTAMNRGQVLYRVAELMEG
                     RRAQFIDEVSAAEGITSGQAERAVDRTIDRWVWYAGWADKISQVLGTVNPVGAPYFNF
                     TIPEATGIVGVIAPEGSSLLGLISRIAPAVVGGNVVVALASESRPLPAVTLGEVLATS
                     DVPPGVINILTGWRSELVPILAAHMDVNALDIWGVPREMRQEVEVSAMDSVKRVARPP
                     RSGEERFDWFDDRVAQRPEWIARYLEMKTVWHPIGI"
     gene            3645..>4156
                     /locus_tag="VMZ33_08350"
     CDS             3645..>4156
                     /locus_tag="VMZ33_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUR17282.1"
                     /translation="MRSPARQPAVVAATVLIPFALLAIWAKLYSPASWEAGVLLALNN
                     PQGLAGDIAGGLNGVGNLPVWAVVTAGLALLLGVARGIPAGLLVALSALSDGAAYLVK
                     LLVERDRPDTAATEQFFGSDAFSYPSGHTVRATALVAVVAWLLLPQRWRLPGAIVFGI
                     AAGAAMGWARV"
BASE COUNT          685 a         1389 c         1392 g          690 t
ORIGIN      
        1 ccgcacgcgt gtccgcgctc gtactcgatg cggcaccgaa cgccgcgcgt cgattcgagg
       61 ttgagtgccg agatcggccg gtagaggctc ttgatggtcg cggcgcccag gcccaggtcc
      121 gcgaggtgcc gacggctcag cggcgaagag aagaccccgc aggcacgcca cctgggttcg
      181 gcggcgtagt cgacgacgat cgccgagatg cctcgcagcg ccagccgaat agcggtggcg
      241 gcgccggccg gtccgccgcc gatgatcacg acgtcggccg cgtcaggcat cagcgtcagg
      301 cacgtacgaa tacgagcgcg tagcgatagg cgggtagggc gtcatagcgc gcgacaaggc
      361 gcagcccggc ttgctcggcg agggagcgaa cctcatccac ggagtaggcg cggcgcactg
      421 atagcggcgc gtcgtggcgc gtgtagacgt tgcgtgtcag agcccgggtg agcgcccacg
      481 cgccgatcca ccagtgccag cctggcgcga ggtcgttgat cacgaccgca cctgtggcca
      541 cgcggcgcat ctcgagcagc agcgcgacag cctgatccgc gtccaggtgg tgaagcacca
      601 gcgagcagtg agcgacatcg acagacccgt ccggggagtc gatccggtcg ggtggatctt
      661 ggcggacctc gaatgatgga ccggccgctg ccgtggcgcg agctacgtcg acaagctcgg
      721 ctcgaacgtc cgtcgccagg acgtggagct gatggcgatg cttgtcacgg cgcagcaaca
      781 tgtggggtat gtctccgcca cccgttccga cgtcgacgat ccgcgtcgtc ctgccgatct
      841 cgatgaacgg tcgaatggcg cgccacgaaa gattggcgcc gcccagccaa cggttgactc
      901 gggccagatc acggaggttg ccactcagga tcacggtgtc gagtggccca tcgaggtact
      961 cggccttcgt cgatcgtcgc accggcgaag cgtagccgct gccggcattg acgttaagtg
     1021 acccgcaagt caccttgagc gcttgtcggt tcgcaccctg acgtgtaccc tgaccctgac
     1081 atggcagata ggcgccgccg gacctccacc accgccactc gcgcgctcgc gccacgtacc
     1141 aggtcgaaca acgtcggtcg tggcacggac tcaccctcgg catggggact cggtgacgga
     1201 caggcggttc catgggatta cgcaccagcg cctgagtcac gcgacgtcgt ccagctcaag
     1261 tcgcgctacg gcctgttcat cggcggacgc gaggtcgctc cccgatcggg gcagtggttc
     1321 accacgatcg atccggctac cgaggagccc ctggcggaga tcgcgcgcgc gacagtcgag
     1381 gacgtcaacc aggctgtgtc ggtggcgcgg cgcgccttca agcgtggctg gggcgacctg
     1441 cccggcagcg agcgcgccaa gtacctgttc cgaatcgctc ggatcctcca ggagcgcagc
     1501 cgcgagttcg ccgtgctcga atcgctcgac ggcggcaagc ccatcaagga atcgcgcgac
     1561 gtcgacgtgc cgctcgcggc tgcccacttc tggtattacg caggctgggc ggacaagctt
     1621 gagtacgcat tccccaacac cgagccgcga cccctgggcg tcgcggcgca ggtcatcccg
     1681 tggaacttcc cgctcctgat gctcgcctgg aagattgcgc cagcgctcgc ggcgggcaat
     1741 acggttgtcc tcaagccggc cagctccacc ccgctgtcgg ccctcttctt cgccgatgtc
     1801 gttcgccagg ctgagctgcc ggcaggcgtg gtcaacatca tcaccggacc cggtgagatc
     1861 ggtctcgccc tggtcaagca ccccgacgtc gacaaggtcg ccttcaccgg ttcgaccatg
     1921 gtcggtaagg agatcggacg cgcgattgcc ggaagtgaca aagcgctcac cttggaactg
     1981 ggcggcaagg cggccaatat cgtcttcgat gacgctccga tcgaccaggc aatcgagggc
     2041 gtggtcaacg gcatcttctt caaccagggc gaggtctgtt gcgccggcag ccggctcttc
     2101 gttcaggaat cgattgccga gcccttcatc gagcgcctca aggaccgcct ctcaacgttg
     2161 cgagtgggcg atcccctcga caagaacacc gatgtcggtg cgatcaacaa ctctgcgcag
     2221 ctggccaaga tcgaggaact gatcgaggcc ggccaggccg agggcgccga catgtaccag
     2281 cccgcctgcg accttccgac gcgcggctac ttcgtacgcc caacgctgtt caccaacgtg
     2341 gcccaatcca gtcgcatcgc gcaggaagag attttcggtc cggtcctgtc ggtgctcacg
     2401 ttccggacgc cggcggaggc catcgaaaag gcaaacaaca ccgcatacgg gctctctgcc
     2461 ggagtgtgga cggacaaggg cagccgcatc ctgtcgatgg tttcgcagct gcgcgccggc
     2521 gtcgtctggg caaacacgtt caaccgcttc gacccgacga gccccttcgg cggctacaag
     2581 gagtcgggct tcgggcgaga aggcggccta cacggcctgc tctcgtacat ggacaccggg
     2641 gaccactgat ggcagcgacc aggcagcgtc gcccggcggc cactgaggcc gtcgtcacgc
     2701 ccaaacaggc gcgcatcccc gttcgcaaga cgtacaagct ctacgttggc ggccagttcc
     2761 cgcgctccga gtccggcagg tcgtacctcg cgaaggcccc agatggcacg ccgctcgcga
     2821 acgctgtcag gtcctcgcgc aaggacgttc gtgacgcggt gcgcaccgcg cgtggcgcgg
     2881 ctggcggctg ggcgtccaaa actgcgatga atcggggcca ggtcctctat cgcgtggccg
     2941 agctcatgga agggcgacgg gcccagttca tcgacgaggt gagtgcggct gagggcatca
     3001 catcagggca ggctgagcgg gctgtggatc ggaccatcga ccgctgggtc tggtatgccg
     3061 gctgggcgga caagatcagt caggtgctcg gcaccgtgaa ccccgtcggc gcgccgtact
     3121 tcaacttcac gatccccgag gcgaccggca tcgtcggcgt tatcgctcct gagggctcat
     3181 cgctgctggg cctgatctcg cggatcgcgc cggcggtcgt gggaggcaac gtcgtcgtcg
     3241 cacttgcctc ggagtcgcgc ccattgccgg ccgtaacgtt gggcgaggtg ctggcgacct
     3301 ctgacgtgcc gcccggcgtg atcaacatcc tgaccggctg gcggagcgag ctcgtgccca
     3361 tcctcgccgc acacatggac gtcaacgcgc tcgacatctg gggagtgccg cgcgagatgc
     3421 gccaggaggt cgaggtgagc gccatggaca gcgtcaagcg cgtagcccgt ccgccgcgga
     3481 gtggcgaaga acgctttgac tggttcgacg accgagttgc tcagcgtcct gagtggatcg
     3541 cccgttacct cgagatgaag accgtctggc atcccatcgg gatctgacgc ccggcagccg
     3601 ggcgggctaa tgacgagctt ttattccttg tcagactcca tcgggtgagg agccccgcgc
     3661 gccagcccgc cgtcgtcgcg gccacggtgc tcattccctt cgcgctgctg gccatctggg
     3721 ccaagctcta ctcgccggca tcgtgggagg ctggcgtcct tctcgcgctg aacaacccgc
     3781 agggactcgc gggcgacatt gccgggggac tcaacggtgt cggcaacctg ccggtctggg
     3841 cggtcgtgac tgccgggctc gctctcctgc tcggagtggc acgtggtatc ccagcgggtc
     3901 tcctcgttgc cctgagtgcg ctgtctgacg gcgcggcgta tctggtcaag cttctcgtcg
     3961 aacgtgaccg tcccgacacg gccgcgacgg agcagttctt tggctccgac gccttctcct
     4021 acccgagtgg ccacaccgtc cgcgcgacgg ccctcgtagc cgtcgtggca tggttgctgc
     4081 tgccgcagcg ctggcgacta cccggcgcca tcgtgttcgg tatcgcagct ggcgcggcca
     4141 tggggtgggc acgcgt
//
LOCUS       DATLCO010000363         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10336912, whole genome shotgun sequence.
ACCESSION   DATLCO010000363 DATLCO010000000
VERSION     DATLCO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10336912"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..138
                     /locus_tag="VMZ33_08355"
     tRNA            63..138
                     /locus_tag="VMZ33_08355"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Cys,seq:gca)
     gene            complement(105..812)
                     /locus_tag="VMZ33_08360"
     CDS             complement(105..812)
                     /locus_tag="VMZ33_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HUR17283.1"
                     /translation="MISASHLAAVPNAALLVREYVDALLDDSRSPGDADRAISQALEK
                     GADPMWILLHLIPAALHEIGDRWQAGEISVGDEHLATGIVQGTMRDVARRLTRSSRLD
                     RAIVVAAVGGELHDNGARLLSDMLDAHGWDAFFAGAATPVADLVRLIEERRPAAVALS
                     ITLPRYMPALEAAVRALKSQVSDAPFVVVGGQGCAGQSDLAERLGADAHARTIDEALL
                     LLSIGKSGGDDQIRTGA"
     gene            complement(809..1369)
                     /locus_tag="VMZ33_08365"
     CDS             complement(809..1369)
                     /locus_tag="VMZ33_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUR17284.1"
                     /translation="MDARQDNADEPYARLVELIATGDMDAVGPLYDSIAPQVYGLALR
                     ALRDPSLAEDAVQDTFVAIWRNAGRFDRTRGSAKAWLLTIARNRAIDAVRRRRPVTSL
                     PETETAQGNASATWWMDDDRFANRSMLADGLAKLPPAQRTAIQLSFVQGMTHQQIALH
                     TGAPLGTVKSRVRIGLSRLRDHCSVT"
     gene            complement(1468..>2251)
                     /locus_tag="VMZ33_08370"
     CDS             complement(1468..>2251)
                     /locus_tag="VMZ33_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HUR17285.1"
                     /translation="VPLIFLYTDVIPAALTTVGDRWKSGAISAADEHIATGIVRDGLY
                     ELGRRAARRPQRNLTILVAAVEEELHDLPTRIVSDMLTADGWIALCVGASTPIEALAR
                     LVIDREPDAVALSATMPQHLDMLRASVARLRAPDVPPTFIVVGGQALAGLALDSAPLG
                     ADVVELRADRAAAHLAAFAARRRAGPDVSDLTPREQVVLTLIGEGLTNREIAGRLEIS
                     EATAKTHVRHILDKLGVRNRVEAAMFGAAAQSGPSSRIATSL"
BASE COUNT          391 a          721 c          753 g          386 t
ORIGIN      
        1 gttcgaaccc aggagggcca gccaaagatc agtcgacggc gccggcatcg gcgcccactc
       61 gaggcgacgt acccaagtgg ttaagggggc ggtctgcaaa accgctatgc accagttcga
      121 atctggtcgt cgcctccact cttcccaatg ctgagtagca gcagggcttc gtcaatcgtc
      181 cgcgcgtggg catccgcgcc aagccgttca gccaggtcgc tttggccggc gcagccttga
      241 cccccgacga cgacgaacgg ggcgtcgctg acctgtgatt tgagcgcacg gacggccgcc
      301 tcaagcgctg gcatgtagcg cggcagtgtg atcgacagtg ccacagcggc gggtcgacgc
      361 tcctcgatga ggcggaccag atccgcgacg ggtgttgcgg cacccgcaaa aaacgcgtcc
      421 caaccgtgtg cgtcgagcat gtctgataga agtcgcgctc cgttgtcgtg cagctcgcct
      481 ccgaccgcag ccacgacgat tgcacggtcg agcctggagg atcgcgtcag gcgcctcgcg
      541 acgtcgcgca tggttccctg cacgatcccc gttgccaggt gttcgtcacc gactgatatc
      601 tcgcccgcct gccagcgatc accgatctca tgcagcgcgg cggggataag gtgcagaaga
      661 atccacatcg ggtcggcgcc cttttcgagg gcctgagaaa tggcgcgatc ggcatcaccc
      721 gggcttcggc tgtcgtccag gagagcgtcg acgtattcgc gaacgagaag agcggcgttc
      781 ggtacggccg cgagatggga ggcggagatc acgtcaccga gcagtggtcg cgcaaccggg
      841 agagaccgat ccgaacgcgc gacttgaccg tcccgagcgg ggcgcccgta tgaagcgcga
      901 tctgttgatg agtcattccc tgcacgaagg agagctggat cgctgttcgc tgcgcgggcg
      961 ggagctttgc caaaccgtcg gccagcattg accgattggc aaagcgatcg tcatccatcc
     1021 accacgtggc gctggcgttg ccctgcgcgg tctcggtctc ggggagagac gtgacgggac
     1081 gccgtcggcg caccgcatca atggctcggt ttcgcgcgat cgtcagcagc cacgccttgg
     1141 ccgatccccg cgtgcgatcg aaccgcccgg cgttccgcca gatagccacg aatgtgtcct
     1201 ggacggcgtc ttcagccagt gatggatctc tgagagcacg caacgctagc ccgtaaacct
     1261 ggggcgcaat cgagtcgtaa agcggcccga ccgcgtccat gtcgccggtg gcaatgagtt
     1321 cgacgagccg ggcgtaaggc tcatcggcgt tgtcttgtct cgcgtccact tgcacctctc
     1381 tcctctgcca cgctgaccgc gtttggcatg aggaaccgtc gccgaccaga gtggtgccgg
     1441 tcgtcgccag aggggatgac tagggcatta caacgacgtt gcgatccgcg aacttgggcc
     1501 tgactgggcc gcggctccga acatcgctgc ttcgacgcga ttgcggaccc cgagcttgtc
     1561 gaggatatgc ctgacgtgcg tcttggcggt tgcctccgag atctcgagtc ggccggcgat
     1621 ctcacggttt gtcagacctt caccgatgag cgtcaggacg acctgctccc gtggagtcaa
     1681 atcgcttacg tcaggtccgg cacgacggcg ggccgcgaag gccgcgaggt gggctgccgc
     1741 tcgatctgcg cgcagctcga cgacgtcggc gcctagcggc gccgagtcga gagcgaggcc
     1801 ggcaagtgcc tggccaccga cgacgatgaa cgtgggtggg acgtcgggcg cacgcagccg
     1861 agccacggaa gcgcgcagca tgtcgaggtg ctgcggcatt gtcgccgata gcgcaacggc
     1921 gtccggctcg cggtcgatta ccagccgcgc cagagcttct atcggcgttg acgcgcccac
     1981 gcacagtgcg atccaaccgt cggccgtgag catgtcgctc acgattcggg tcggaaggtc
     2041 atgtagctcc tcttcgaccg ccgcgacgag gatcgtcagg ttccgttgcg gccgacgtgc
     2101 agcgcgacgg ccgagctcgt acagaccatc gcgcacgatg cccgtggcga tgtgctcgtc
     2161 ggccgcgctt atggcgccgc tcttccaacg atcaccgacc gtcgtcaggg cggccgggat
     2221 gacgtcggtg tagagaaaga tgagcggcac g
//
LOCUS       DATLCO010000364         3043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6564508, whole genome shotgun sequence.
ACCESSION   DATLCO010000364 DATLCO010000000
VERSION     DATLCO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6564508"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VMZ33_08375"
     CDS             <1..470
                     /locus_tag="VMZ33_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17286.1"
                     /translation="LAAYLVSRAHEIKIVNTVCPVTIRRQQDTMDLAEQVDMMVVVGG
                     RNSANTKELTRLCQIAGKPVVQIEGEGDLVDAAVFDGARVVGVSGGTSTPIEDLEKVA
                     QRVFELAGSEARRPRAAELAHEALAAVAEPAYRSTSLDDRGRHRRKTPVAGAA"
     gene            475..2067
                     /gene="der"
                     /locus_tag="VMZ33_08380"
     CDS             475..2067
                     /gene="der"
                     /locus_tag="VMZ33_08380"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HUR17287.1"
                     /translation="MPIRRKSRPTKSRLEDAPTEGLPVVAFVGRPNVGKSTLFNRVIG
                     DRVAIVEDRARTTRDRLYDVGEWNGRRFIVIDTGGLENRPGDAIEERVQEQARLAIDE
                     ADVIVFVVDAETGPTPADQEANELLRTSTAPVIVAANKSDNPKREFQAAEFHQFGWPE
                     IHPISALHGRGVADLLDVVVWALPAEAETELARKRKEEEAAAAAEPWPDSRGKVEDDE
                     GADDAAFAMDDRPVRIAIIGRPNVGKSSLLNALMGQERAIVSDIAGTTRDAIDTSFEW
                     AGRTIRLVDTAGIKRRGKVAGGPAAERFATLRALKAVTRSDVSVLVVDAQDGLTAQDD
                     HVAGYALEEGTGLVIAINKWDVVEKDEHTFDEYAAEIRQEAPFLDVAPIVAISAKTGQ
                     RVGRVLEAALEIAAERRRRVPTAALTAWLREATLRRPAPNVRGKQPRFFYATQVSIEP
                     PTFVLFASEASTLHFSYKRFLENQLRDAFGFAGTPIRLVIRERTREEGRGRPRAGTKR
                     KAPGAPRGKTSSTRRVSSSGRR"
     gene            2079..>3043
                     /locus_tag="VMZ33_08385"
     CDS             2079..>3043
                     /locus_tag="VMZ33_08385"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HUR17288.1"
                     /translation="MERHPHRAIIGAGQWGTTLALHLARQGPVVLLARDEEQARVLNS
                     ERQNRYLPEITLPVEVEITADPAAVAAADELVIFAVPSKAMRSTARRVSHEIHPDAVL
                     LSVAKGLEADSLLRMTEVITDEVPTATGRVAAMSGPNLAKEIASQLPASAVIAAEDED
                     VTRRASEMIGTRSFRMYRNRDVIGVELCGALKNIIAIAAGASEQLSFGDNGKAGIITR
                     GLAEITRLGLASGANPLTFAGLAGIGDVVATCYSSLSRNHALGVELAKGRKWKDIEGT
                     LAGVAEGAYTVTSALSLASKLGVEMPIAQEVYNALFEGKSVQRCL"
BASE COUNT          528 a          991 c         1063 g          461 t
ORIGIN      
        1 gactggccgc atacctcgtg agccgcgccc acgagatcaa gatcgtcaac accgtctgcc
       61 cggtcaccat ccgccgtcag caggacacga tggatctcgc cgagcaggtc gacatgatgg
      121 tcgtcgttgg cggccggaac agcgccaaca ccaaggagct gacgcggctg tgccagatcg
      181 cgggtaagcc agttgtccag atcgagggcg aaggcgacct cgtggatgca gcggtgtttg
      241 atggcgctcg cgtagtcggc gtcagcggtg gcacgtcgac cccgatcgaa gacctcgaga
      301 aggtcgccca gcgggtcttc gagctcgctg gatcagaggc gcgccgtcca cgcgcggcgg
      361 agcttgcaca cgaggcgctc gcggccgtcg ccgagcccgc ctatcgcagc acgtcgctcg
      421 acgacagagg ccgtcaccgc cgcaagacgc ccgtcgccgg cgcggcataa aggcgtgccc
      481 attcgccgga agagccggcc gacgaaatcg cgcctcgagg atgccccgac tgagggtctg
      541 cccgtcgtcg cgttcgtagg ccgccccaat gtcgggaaga gcacgctctt caaccgtgtc
      601 atcggtgatc gggtagccat cgtcgaggat cgcgcccgaa cgacgcgcga ccgcctatac
      661 gacgtcgggg agtggaacgg ccggcggttc atcgtcatcg acaccggtgg tttggagaac
      721 cgacccgggg acgccatcga ggagcgcgtt caggagcagg cgcggctggc tatcgatgag
      781 gccgacgtca tcgtcttcgt ggttgatgcc gagaccggcc cgaccccggc cgaccaggag
      841 gccaacgagc tcctgcgcac ctcgaccgcg ccggtgatcg tcgccgcaaa caagtccgac
      901 aacccgaagc gagagtttca ggcggcagag ttccatcagt tcggctggcc tgagatccac
      961 ccgatcagcg ccctgcatgg acgaggggtg gccgacctgc tcgatgtcgt tgtgtgggcc
     1021 ctgccggccg aggcggagac cgagctggcg cgcaagcgga aagaggagga ggccgcggcg
     1081 gccgccgagc cgtggcccga ctccaggggc aaagtcgagg acgacgaggg cgcggatgac
     1141 gcggccttcg cgatggatga tcgcccggtt cggatcgcga ttatcgggcg gccgaacgtc
     1201 ggcaagtcat cgttgctcaa cgcgctcatg ggccaggaac gggccatcgt gagcgatatc
     1261 gcggggacga cacgcgacgc aatcgacact tcgttcgaat gggcagggcg cacgatccgg
     1321 ctcgtcgaca cggctggcat caagcggcgc ggcaaggtcg ccggcggtcc ggcggcggag
     1381 cgcttcgcca cgctgcgtgc gctgaaggcc gtgactcgct ccgatgtgag cgtcctcgtg
     1441 gtcgacgccc aggacggatt gactgcccag gacgaccacg tcgccggcta cgcgctggag
     1501 gagggcacgg ggctcgtcat tgccatcaac aagtgggacg tggtcgaaaa agacgagcac
     1561 accttcgacg agtacgcagc cgagattcgc caggaggcgc cgttcttgga tgtggccccg
     1621 atcgtggcca tcagcgccaa gacgggccag cgcgtggggc gcgttctgga ggccgcactg
     1681 gagatcgcgg cggaacgtcg ccgtcgggtt cccacagccg ccctgactgc ctggctgcgg
     1741 gaggcgaccc tgcgccggcc cgcgccgaac gtgcgtggga agcagccacg cttcttctac
     1801 gccactcagg tgtccatcga gccaccgaca ttcgtgctgt ttgcgagcga ggcgtcgacg
     1861 ctccacttca gctacaagcg cttcctggag aatcagctgc gcgatgcgtt tggattcgcc
     1921 ggcacgccga tccgactcgt catccgcgag cggacgcgcg aggagggccg cggccgcccg
     1981 cgcgcgggca cgaagcgcaa ggccccaggt gcgccgcgtg gcaagacgtc ctcgacgcga
     2041 cgcgtcagca gctcgggtcg ccgctagagt tcggcgcgat ggaacgccac ccgcatcgcg
     2101 cgatcatcgg ggcagggcag tggggaacta ctctcgcgct gcacctggca cggcagggcc
     2161 ccgttgtgct gcttgctcgc gacgaggagc aggcccgcgt cctgaacagc gagcggcaga
     2221 atcgctacct ccccgagatc acgctcccgg ttgaggtcga gatcacagcc gatcccgccg
     2281 ccgtggccgc ggccgacgag cttgtgatct tcgccgtccc atcaaaggcg atgcgttcga
     2341 ctgcccggcg ggtttcccac gagatccacc ccgatgcggt gctgctgtca gtagccaagg
     2401 gtcttgaggc cgactcgctc ctgcggatga cagaggtgat caccgatgag gttcccaccg
     2461 ccacagggcg cgtcgcggcc atgtcggggc cgaacctggc aaaggagatt gccagccagc
     2521 tgccggcctc ggcagtgatc gcggccgagg atgaggacgt gaccaggcgc gcgagcgaga
     2581 tgatcggcac ccgctcgttc cgcatgtacc gcaaccggga cgtaatcggc gtcgagttgt
     2641 gcggggcgct caagaacatc atcgccatcg ccgccggcgc gtctgagcag ctcagtttcg
     2701 gcgacaacgg caaggccggg atcatcactc gtggcctggc ggaaataacg cgcctgggcc
     2761 tcgcttcggg tgccaacccg ttgacctttg ccgggctggc ggggatcggc gatgtcgtcg
     2821 ccacctgcta ttcgtccctg tcgcgcaacc atgcgctggg tgttgagctg gcaaagggtc
     2881 ggaagtggaa ggacatcgag ggcacgttgg cgggcgtggc ggagggggcc tacacggtca
     2941 catcggccct gtcgctcgcg tccaagctcg gcgtggagat gccgatcgcc caagaggtct
     3001 acaacgcctt gtttgagggc aagagcgtcc agcgctgcct cat
//
LOCUS       DATLCO010000365        12528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_10382414, whole genome shotgun sequence.
ACCESSION   DATLCO010000365 DATLCO010000000
VERSION     DATLCO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12528
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10382414"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VMZ33_08390"
     CDS             <1..193
                     /locus_tag="VMZ33_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17289.1"
                     /translation="VPTALAPAKPGDYFDAHFDAEEDAIVFRRLGGRENWLAVMKECP
                     VSPDDVPPRRRERRKRRKL"
     gene            190..609
                     /locus_tag="VMZ33_08395"
     CDS             190..609
                     /locus_tag="VMZ33_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUR17290.1"
                     /translation="MSWLLDTDVICQPAKKKGDPKVIAWLEVEQDRCYTSAVVIAQLA
                     YWVRTKEGRQRHTLQAWLTRLVDALQGRIHGFNVSVAHVWAEQERLLEKAGQRMPVED
                     SYIAATARRHGLTLATGNDRDFKRPGLKVFNPFKELP"
     gene            1057..2922
                     /locus_tag="VMZ33_08400"
     CDS             1057..2922
                     /locus_tag="VMZ33_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HUR17291.1"
                     /translation="MAMLRGVGWPTAMAAWFHAVVHRGVRRGHGRTSPLGLLVLMALC
                     CGAVSCTSEPPAPAAEPSHSLTDGSPAAVIPPGEDWFVDRAATAGIDFSHYNGMSGHF
                     YQPEMMGPGVAMFDYDGDGDLDVYLVQGGRLGTAPPLLAVPKGPLTDRLYRNDLVVHA
                     DSTRTVHFTDVTERSGLRPRGYGMGVTTGDVDNDGKIDLYLTGFGRNQMFRNNGDGTF
                     QDVTARSGISDLSSWSVSAAFVDIDRDGWLDLFVGNYLNYTLQNHTPCFSLTGPEDYC
                     PPGVYRPRASRLYRNKGDGTFLDVTAAAGLERDFGPALGVATADFNGDHWIDLFVADD
                     QHENQLWVNQHDGTFRNMAPLWGAAAGPNGELKANMGVDAGDFDNDGDDDLFITELTG
                     QGSTLYVNGGQGLFDEQSARQGIRGPSLPYTGFGAAWIDIDNDGWLDIVSVNGSVTQN
                     LETLGPDNPFPLQQRNQVFLNRRAEGFAEVTDRAGAAFRLSDVSRGAAFGDIDNDGDM
                     DVLIANAGGPARLLVNQIGARHHWLGLRLVGGRARRDMVGAIAAVTLDDGSARWRRAR
                     ADGSYASANDPRVLVGLGESAGPVQLRVTWPSGRVESWSSVPVDRYTTLTEGEGR"
     gene            2919..4541
                     /locus_tag="VMZ33_08405"
     CDS             2919..4541
                     /locus_tag="VMZ33_08405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR17292.1"
                     /translation="MTASTARRGVIVVACLLAIWIGGCARRDESAPSVATSGSTQTAA
                     EAARRPITLPDVSRLAASVQTQVRARHAALVAKLENRTTPPAELGAAYGDLGLVLMAS
                     EFYQLAASCYLSAQALVPDDARWPYYLGHLYRLQGEPVKAAQFFSRALDLQPADSPTL
                     IWLGETYLDQNRPDQAEPMFLRALSQQPGSAAALSGVGRAALAKQDFARAVDHLERAL
                     AAEPRALSLHYSLAAAYRSMGQLDRASAHVAKRGTGRTTPRDPLMDAYDAVLQSPLNF
                     ETQGLRALESGQVAQAADLFRKGLALAPDDPKLLHRLGTALFMQGDAPGSVRLFEEAL
                     RRAPDFPRAHFGLGMVLNLTGQKAGAIEHFAAAVKYQPDYLEARLALADALGSAGRLQ
                     ESLSQFARIVELDPGLAEAWGMYARTLVIMKRYREARDRLNEALRIHPSDAALTDLLV
                     RLLAAAPDDQLRDGRQAMALLQELLKSSPPVDVREPMAMTLAELGQYEEAVRWQREAI
                     AAATRAGRNDVARLMSANLALYERRRPCRNPL"
     gene            4557..6794
                     /locus_tag="VMZ33_08410"
     CDS             4557..6794
                     /locus_tag="VMZ33_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR17293.1"
                     /translation="MGESADAVPPRLPERFLPIAMAVFVVSLTVRLVHLFQIRRAPFF
                     ALLMGDAQSYHTWAQQIAAGDWVGTDVFYQAPLYPYLLGLVYTLLGEGPLTVRLFQAA
                     IGSLACVLLAYAAWRLFSKPAGLAAGLMLAVYAPAIFFDGLIQKSVLDGFLLCLTLAL
                     LTRLLTQPSRPVSWLWVGAAVGCLTLSRENALVLAAAMLVWLFRKPPLVSKERLVPAA
                     WLLAGLAIVLLPVAIRNRVVGGEFQLTTSQFGPNFYIGNNESSSGVYQPLRFGHGDPR
                     YERQDATELAQQATGRTLSGGEVSEYWTRRALDYIRTQPGDWLQLTGRKIMLVWNAAE
                     VADTEDELTYADWSIVLRVAGWVFHFGVLAPLALLGLWATWPRRGELWLLYLLPAAYA
                     VTLVAFAVMARYRYPLVPFLILFAAAGVTTARRLLLATRPQLASGVVAIVAVAVFCNW
                     RVYSMADMRSITDANVGTELQAQGRLDEAIALYRAALLRDPNDALTYNNLGTALAAKG
                     QLDEAIVHYRRALALAPKDADGHYNLANALMAQGKLADAADQFQEALRIEPGLVEAHL
                     NLGNALSELGRSDEAAGHYRQAIAMRPDMVEAVNNLGLVLSDRGQLDEAIAQFRRALA
                     IDPDFADAHSNLGSALAQTGANAEALTHLQRAVALLPRSADAHNELGVVLAQQNRLDE
                     AIGEFRQAIGLEPGLVAAHGNLGMALQLQGKLDEAIQQYQEATRLAPDNQEMQRRLQA
                     ARPAR"
     gene            complement(6841..7302)
                     /locus_tag="VMZ33_08415"
     CDS             complement(6841..7302)
                     /locus_tag="VMZ33_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HUR17294.1"
                     /translation="MAAADRIYADPSALLALYIHEPASAATSAWRWRTTGALPLTLHG
                     RLEIVNGIGLAGFRKAISAEAMKDAMESFEEDIADGQYVETDVLWRATLRRASELSAA
                     HTPAIGCRTLDVLHVATALELELPFFLSLDVRQRRLAKTAGLRLVQLPQSP"
     gene            complement(7289..7543)
                     /locus_tag="VMZ33_08420"
     CDS             complement(7289..7543)
                     /locus_tag="VMZ33_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR17295.1"
                     /translation="MARTASVRDLRNRFPHVKKLLEQDGEVVVTEQGTPKYRLTPYSV
                     ASRTPSAARKDYLERMRRHQPRPLSAAATRSLHDENRGRR"
     gene            complement(7621..9735)
                     /locus_tag="VMZ33_08425"
     CDS             complement(7621..9735)
                     /locus_tag="VMZ33_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR17296.1"
                     /translation="MSRSTRVGAQRSILRMGVCLAVGALATGTLAAQATIGAAGQRIG
                     EPLTFARDIAPILYQHCAVCHRPGALGPMSLLSYQEVRPWARAIREQVSARRMPPWKA
                     EPEHGGPFIGDRRLPDELVALIERWVAEGAIEGRAADLPPAPVWPDGGWRLGTPDLIV
                     EMAPYPLPAGGPDVLRKFAIPIPIDRTRYVKGVEFQPGNARVVHHANMRIDPTAASRT
                     LDAADPASGYEGVTPFAARFPPGYFLGWTPGQLRPLAPDTMAWRVDAGSDMLLELHLM
                     PGSRPEQVKGRIGLYFADTVPTRIPASIRLGRQNLDIPAGATAHVSRDSYVLPVDVEV
                     LGVQPHAHYLARDIKGYATLPDGTRKWLIAIKDWDFDWQDFYEYEKPFVLPRGTELVM
                     EITYDNSAGNRRNPNAPPTRVTWGQQSSNEMGDLWIQVLPRTAADLQTLNSDRSRREL
                     EEDIVGSEMILAAHPELVIEHNEVALHYLQFGKVAEAVAHFRESARLQPESAAAQYNL
                     GTTLMQGGEHAEAIRHFEQALRLNPGYVAAHNSLGAALRLQGRAGEASGHFRQALQLR
                     PGDADALYNLASVLADQGDAEAAIAYYRRAIAQEPEAFDALVELAWLLAAHPNSTLRN
                     PGEAVRLAERASRAPARQDIAVLDVLAVAYASADQFDRAVAAARRALAALPADARGSE
                     LEKAIQRRLDLYAQGRPYRGRR"
     gene            complement(9752..11524)
                     /locus_tag="VMZ33_08430"
     CDS             complement(9752..11524)
                     /locus_tag="VMZ33_08430"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR17297.1"
                     /translation="MARGLSSAGVVTSALLACVCVCAAIAPLAAHAKQSSLRRLVSHV
                     PRVTAYAPMQGAAGEPALLPVNLPDLTGMHPSVQQQLRDAYAALQNLLPSGRDGGRRR
                     SASRQVERGEAYGTLGKLLMAARYPDVAERCLRNAQTLAPSDRRWPYYLGHLFITAGA
                     LTEAVTPLEQALELRPDDFATLVWLGHVHIELGQPALAEPFLNRARALAPEAAAVWYQ
                     LGRASLAKRDFASAVTYLEQALRLNPAAAVIHQPLAMAYRGLGDLDKAQSNLDRIAGR
                     GGAGATVTMADPLMAELNTILRSPDVFAELARQASARGQWLEAATGFRKAIDLAPANA
                     GYRVGLAQTLNRLGDARAAMSELDKAIAVDPSLAAAHLLKAVLLERGGRDERAIAEYT
                     AAATHDPTLREAHVRLADALRRVGRLDAALSSYRRVLELAPDAEDARFGEAMALAQLS
                     RHAEARGRLRDAMMRHPDQPAFAQALARLLAASPDPQVRDGRLALDLVTSLANQHKTT
                     SVAETMAMALAEVGRFEDAVEWQRLAMSVAADAGHSDAAKRMAANLALYQRHEPCRTP
                     WRDDDPEYRPGPVVDPNLLDPNPL"
     gene            complement(11539..>12528)
                     /locus_tag="VMZ33_08435"
     CDS             complement(11539..>12528)
                     /locus_tag="VMZ33_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HUR17298.1"
                     /translation="DVTAEAQVGRESGPALGVVAADFNADGWADIYVTNDGFPNFLWM
                     NQQNGRFRNTALLAGAALNGLGSAQGSMGVDAGDVDNDGDEDLFVTNLPGESSTLYVN
                     DGSGLFDDQTARAGLGIPSMPHTGFGTAWFDFDNDGWLDLFVANGSVKIQERAGRVAA
                     PFPLDERNQLFRNLGNARFEDVSTRAGAVFELSEVSRGAAFGDVDNDGDIDVLVTNNN
                     GPARLLINHVGERGRWLGLRLVGGTGGRDMLGAKVGVFRSQGPPLWRRARADGSYASA
                     NDPRVLVGLGNAATIRRVLVVWPNGREEEWTDVGVGRWTTLKEGSGRRPGGGG"
BASE COUNT         2016 a         4139 c         4251 g         2122 t
ORIGIN      
        1 ggttccgacc gccctcgcac cggcgaagcc gggcgactac ttcgatgcgc acttcgacgc
       61 cgaagaggac gcgatcgtgt tccgtcgcct gggagggcgc gagaactggc tcgcggtgat
      121 gaaggagtgt cctgtgagcc cggacgacgt gccgccgcgg cggcgcgagc ggcggaagcg
      181 gcggaagctg tgagctggct gctcgacacg gacgtcatct gtcagccggc gaagaagaag
      241 ggcgatccca aggtgatcgc ctggctggag gttgagcagg atcgctgcta caccagcgcc
      301 gtcgtcatcg cgcagttggc gtactgggtg cgaacgaagg aaggccggca gcgtcacaca
      361 ttacaggcgt ggctcacacg gctggtggac gcgctgcagg gccgtatcca cgggttcaac
      421 gtgtcggtgg cgcacgtctg ggcggagcag gagcgactgc tcgagaaggc cggtcagcgc
      481 atgcccgtcg aggacagcta catcgcggca acggcgcgac gacatggatt gacgctcgcg
      541 acgggaaacg accgtgactt caagcgaccc ggactcaagg tcttcaaccc gttcaaggaa
      601 ctgccgtagc cgagcgtgga gtcggcgaaa tagtggcgaa acgcgctgag ttgacgccgc
      661 ttctcgactc aaccagttgc gaaatcgtcc agtgcggcga gccaactcca tggcattcgc
      721 tcagtcgtcg ccacaagtcg ccagtcatca gtcgccagtc ccgctcggtt cgcgcgccgc
      781 gacgtacggc gaacctcggt ggacacccaa gaccggcaat tagtcgacac ctcaaaaccg
      841 acaactcact gacaacggcc gagacattga cggcggcggc ggagctgtag tccttcgcgg
      901 gcaggagcga cgtcttggag ccacaggagc agcaagaagt tctggcgctc ggtcggttgg
      961 ggacattctc cagtaatcac tggtccgaag aatgccggca ggtcaccaca ccaccgcgag
     1021 ccgcgtctct ccgaacgtgt ggcatgattc gacgccatgg cgatgcttcg gggcgttggg
     1081 tggccgacag ccatggcggc ctggttccac gccgtcgttc accgtggggt ccgccgaggt
     1141 catggtcgca cctcgcccct gggcctgctc gtcctcatgg cgctgtgttg cggagcagtg
     1201 tcctgcacgt ccgagccccc cgcaccggcg gccgaaccat cgcactcgtt aaccgacgga
     1261 agccccgcgg ccgtcatccc gcccggcgag gattggtttg tcgatcgcgc ggcgacggcg
     1321 gggatcgact tctcccacta caacgggatg tcggggcact tctatcagcc tgagatgatg
     1381 ggaccgggcg ttgcgatgtt cgactacgac ggcgatggcg atctcgacgt ctatctcgtg
     1441 cagggcggcc ggctcgggac cgccccgccg ctgctcgccg tgccgaaggg accactgaca
     1501 gaccggctgt atcgaaacga tctggtggtg catgccgaca gcactcggac cgtgcacttt
     1561 accgatgtca ctgagcgtag tggactgcgt ccacgcggat acggcatggg cgtcacgacc
     1621 ggggacgtcg acaatgacgg caagatcgac ctctacctca ccggctttgg acgcaatcag
     1681 atgttccgga acaacggcga cggcacgttc caggatgtga cggcgcgatc aggaatcagc
     1741 gacctctcgt cctggagcgt ctctgccgca ttcgtggaca tcgatcggga cggatggctg
     1801 gatctgttcg tcggcaacta tctgaactac acgctccaga accacacccc gtgcttcagt
     1861 ctgaccgggc cagaggacta ctgtccgccg ggtgtctatc gcccgcgggc aagtcgtctc
     1921 tatcggaaca agggcgatgg gacgtttctg gacgtgaccg ccgcagccgg cctggagcgc
     1981 gacttcggac cggcgctcgg cgtcgccact gccgacttca acggcgacca ctggatcgat
     2041 ctcttcgtcg ccgatgacca gcacgagaac cagttgtggg tgaaccagca cgacggcacc
     2101 ttccggaaca tggcgccgtt gtggggcgca gctgcgggtc cgaacggtga gctcaaagcg
     2161 aacatgggcg tcgacgccgg cgacttcgac aacgacggcg atgatgatct gttcatcacg
     2221 gagttgaccg gtcaaggcag cacgctctac gtcaacggcg gccagggcct gttcgatgag
     2281 cagagcgcgc ggcagggcat tcgtggaccc agccttccct acaccggctt cggtgccgcc
     2341 tggatcgaca tcgacaacga cggctggctg gatatcgtgt cggtcaacgg ttcggtgacg
     2401 cagaacctgg agacgctcgg cccggacaat cccttcccgc ttcagcagcg caaccaggtc
     2461 ttcctcaacc ggcgcgcgga agggttcgcg gaggtcacag atcgtgcggg ggcggcgttc
     2521 cgtctgtcag acgtgagccg aggtgcggcg tttggcgaca tcgacaacga cggcgacatg
     2581 gatgtcctga ttgccaacgc gggcggaccc gcgcgtctgc tcgtcaacca gatcggcgcc
     2641 cgccaccatt ggctgggcct gaggttggtc ggtgggcgcg cgcgccgcga catggtgggt
     2701 gcgatcgcgg ccgtcacgct ggacgatgga tcggcgcgct ggcgccgcgc gcgggctgat
     2761 ggcagctacg cctcggcaaa cgatccgcgc gtgctcgtcg gcctgggcga gtcggccggg
     2821 ccggtgcagc tgcgcgtcac ctggccgagc ggccgcgtcg aatcgtggtc cagcgtgcct
     2881 gtcgatcgct acaccacgtt gaccgaaggt gaaggacggt gacagccagc actgctcggc
     2941 gcggcgtaat cgtcgtggcg tgcctcctcg ccatctggat cggcggctgc gctcgtcgcg
     3001 acgaatcggc gccatcggtc gccacctcag gctcgaccca gactgcggcg gaagcggcgc
     3061 gccgccccat cacgttgcca gacgtgtcgc ggctggccgc gtccgttcag acacaagtgc
     3121 gagcgcggca cgcggcgctc gtggcgaaac tcgagaatcg caccacccca cccgcggaac
     3181 tcggcgcggc ctacggcgat ctcggcctgg tgctgatggc ctccgagttc tatcagctcg
     3241 ccgcctcgtg ctatctgtcg gcgcaggcac tggtccccga cgatgcgcga tggccctact
     3301 atctcggtca cctctatcgg ctccagggag agccggtgaa ggcggctcag ttcttctcgc
     3361 gggcgctgga cctgcaacca gcggactcgc cgaccctgat ctggctcggg gagacctacc
     3421 tggatcagaa ccggcccgac caggccgagc cgatgttcct tcgcgcgctg tcgcagcaac
     3481 ccggctccgc ggcagcactc tcgggtgtcg ggcgtgcggc gctggcgaag caggatttcg
     3541 cgcgcgccgt cgatcatctc gaacgcgccc tggccgccga gccgcgggcg ctcagcctgc
     3601 actactcgct ggcggcagcc taccgcagca tgggccagct cgatcgtgcg agtgcgcacg
     3661 tggccaagcg gggaaccggg cgcacgacac cgcgcgatcc gttgatggac gcctacgacg
     3721 cggtgttgca gagccccttg aacttcgaaa cccaggggct gcgagcgctc gagagtggac
     3781 aggtggcaca ggcggctgat ctcttccgga aggggctggc gctcgcaccc gatgacccga
     3841 agctgctcca cagactgggc accgcgttgt tcatgcaagg tgacgcgccc ggttccgtgc
     3901 ggctgttcga agaggcactg cgtcgagcgc ccgatttccc gcgagcgcat ttcggtctcg
     3961 gcatggtgct caatctgacc ggccagaagg ccggagccat cgaacacttc gccgccgcgg
     4021 tgaagtacca gccggactac ctcgaggcac ggctggcgtt ggccgacgcc ctcggatctg
     4081 ccggacgtct gcaggaatcg ctctcacagt tcgcgcgcat cgtcgagctc gatccgggcc
     4141 tcgccgaggc gtggggcatg tacgcgagaa cgctggtcat catgaagcgg tatcgggaag
     4201 cccgcgaccg gttgaacgag gcgctgcgca ttcatccctc cgacgccgca ctgaccgacc
     4261 tgctggtgcg cctgctcgcg gcagcccccg acgatcagct gcgcgacggg cgccaggcca
     4321 tggcgctgct gcaggagctg ctcaagagct cgccgcccgt cgacgtgcgc gagccgatgg
     4381 caatgacgct ggcggagttg gggcagtacg aggaagccgt ccgctggcag cgtgaggcga
     4441 tcgcagcggc cacccgggcg gggcgtaacg atgtcgcccg actcatgtca gccaaccttg
     4501 cgctgtacga acgacgccga ccatgtcgaa atccgctgtg aggggccgac accgacatgg
     4561 gtgagtccgc cgacgccgta ccaccccgtc tgccggagcg gtttcttccg atcgcgatgg
     4621 cggtcttcgt tgtcagcctg accgttcggc tcgtacacct gttccagata cgacgggcgc
     4681 ccttcttcgc gctgctcatg ggcgacgcgc agagttacca cacgtgggcg cagcagatcg
     4741 ccgccggtga ctgggttggc accgacgtgt tctaccaggc cccgctctat ccctatctgc
     4801 tgggcctcgt gtacacgctg ctcggcgagg gaccgttgac cgtgcggctg ttccaggccg
     4861 ccatcggttc gctcgcctgc gtgctgctcg cctacgccgc gtggcgtctg ttctcgaagc
     4921 cggcgggcct cgccgccgga ctgatgctgg cggtctatgc tcccgcgatc ttcttcgacg
     4981 gcctgattca gaagtcggtg ctagacggct tcctgctgtg cctgacgctg gccctgctca
     5041 cacggctgct gacgcagcct tctcgtcccg tctcgtggct gtgggttggc gcggcggtcg
     5101 ggtgcctgac cctgtcccgc gagaacgcgc tggtgctcgc cgcagccatg ctcgtctggc
     5161 tgttccggaa accgcctctc gtgtcgaaag agcggctcgt tccggccgcg tggctgctcg
     5221 ccggactggc gatcgtgctc ctgccggtcg ccatccgaaa ccgcgtcgtc ggcggtgaat
     5281 tccagttgac gacgtcgcag ttcggtccga acttctacat cggcaacaac gagtcgtctt
     5341 ctggcgttta ccagcctctc cgattcggcc acggcgatcc cagatacgag cgacaggacg
     5401 ccacggaact cgcgcagcag gcgacgggtc gaacgctgag cgggggcgag gtgtccgagt
     5461 actggacgcg gcgggcgctc gactacatcc ggacgcaacc tggcgactgg ctccagttga
     5521 ccgggcggaa gatcatgctg gtctggaacg cggcggaggt cgccgacacg gaggacgaac
     5581 tcacctacgc cgactggtcg atcgtcctgc gcgtcgcggg ctgggtcttt cacttcggcg
     5641 tgctcgctcc gctcgccttg ctcggcctct gggcgacgtg gccacggcgc ggcgaactct
     5701 ggctgctgta tctgctgccg gctgcctacg ccgtaaccct cgtggccttc gccgtgatgg
     5761 cgagataccg ctatccgctg gtgccgttcc tgatcctgtt cgccgcggct ggagtgacga
     5821 ccgcgcgccg gctcctgctg gcgacgcggc cgcagctcgc ctccggcgtg gtggcgatcg
     5881 tcgccgtcgc ggtgttctgc aactggcgcg tgtactcgat ggctgacatg cgttcgatca
     5941 cggacgccaa cgtcggcacc gaactgcagg cgcaggggag gttggacgag gcgatcgcgc
     6001 tctatcgtgc ggcactgctg cgcgatccca acgacgcgct cacgtacaac aacctcggca
     6061 cggcactcgc ggccaagggg cagctggacg aggcgatcgt gcactatcga cgagcgctcg
     6121 cgctggcgcc gaaagacgcc gacggccact acaatctcgc gaatgcgctg atggcgcagg
     6181 ggaagctcgc ggacgccgca gatcagtttc aggaggcgct gcggatcgag cccgggttgg
     6241 tcgaggcaca cctcaatctg gggaacgcgt tgagcgagct cggacggtca gacgaagcgg
     6301 ccggtcacta tcgccaggcg attgcgatga ggccggacat ggtggaagcg gtcaacaatc
     6361 ttgggctcgt gctgtccgat cgaggccaac tcgatgaggc gatcgctcag tttcgtcgcg
     6421 cgctggcgat cgatcccgac ttcgcggacg cgcactcgaa cctggggtcg gcgctggcgc
     6481 agacgggagc gaacgcagaa gctctgaccc acttgcagcg cgcggtggcg ctgttgcccc
     6541 ggtctgccga cgcgcacaac gagctggggg tcgtgctggc gcagcagaac cgtctcgatg
     6601 aggcgatcgg cgagttccgg caggcgatcg gactcgagcc ggggctcgtc gcagcgcacg
     6661 gcaatctcgg tatggcgttg caactgcaag gcaagctgga cgaggcgatt cagcagtatc
     6721 aggaggcaac ccgcctcgcg ccggacaatc aggagatgca acggcgtctg caagcggcac
     6781 gtcccgcgcg ctagatcagc gagccttcga gattcgtgtg gccgacttcc gcgttgtgcg
     6841 tcatgggctc tgaggcaact ggacaagcct gagcccggcg gtcttggcca gccggcgttg
     6901 acgcacatcg aggctcagga aaaacggcag ttcgagctca agtgccgtcg ccacgtggag
     6961 cacgtcgagg gtccgacatc cgatggcggg cgtgtgcgcg gcgctcagct ccgaggctcg
     7021 ccgcagcgta gcccgccaga gcacgtcggt ttcgacgtat tgcccgtcgg cgatgtcctc
     7081 ttcgaacgac tccatcgcgt ccttcatggc ctctgcgctg atcgccttcc gaaacccggc
     7141 gagaccgatg ccgttcacga tttcgagacg gccgtgcagc gtcagcggca gtgcgcctgt
     7201 cgtgcgccat cgccaggcgc tcgtggcagc ggatgctggc tcgtggatgt aaagcgccag
     7261 gagcgcgctc gggtcggcgt agatgcggtc agcggcggcc acggttttcg tcgtgcagcg
     7321 atctcgtcgc ggccgcgctg agcgggcgcg gctggtggcg acgcatgcgt tcgaggtaat
     7381 cctttctagc tgccgaaggc gtgcggctcg caaccgagta gggagtgagc cgatacttcg
     7441 gcgtcccctg ttcggtgacg acaacttcgc catcctgttc cagcagcttc ttgacatgcg
     7501 ggaaacgatt gcgaaggtcg cgaaccgatg ctgtgcgtgc catgcgtgag acgagtatgt
     7561 ctcacgcgtc aaaagatcgt caatggccca ccgtgacggg cgccgagtgt ggtcctcctg
     7621 ttaccgccgt ccgcggtacg ggcggccctg agcatagaga tccaggcggc gctggatcgc
     7681 cttctcgagc tcgctgcccc gggcgtcggc aggaagagcc gcgagcgctc ggcgagccgc
     7741 cgcaacggct cgatcgaact ggtcggcgga cgcatacgcc acggccagca cgtcgagaac
     7801 cgcgatgtcc tgtcgcgcgg gcgcgcgaga cgcccgttcc gccaggcgga cggcttcgcc
     7861 cggattgcga agcgttgaat tcgggtgtgc ggccagcagc cacgccagtt ccacgagcgc
     7921 gtcgaaggcc tccggctcct gcgcgatggc gcgcctatag taggcgatcg ccgcctcggc
     7981 atctccctgg tctgccagca cgctcgcgag gttgtacagc gcgtcggcat cgcccgggcg
     8041 aagttggagc gcctgacgga agtgaccact ggcctcgccc gcccggccct ggaggcgcag
     8101 tgcagctccc aggctgttgt gcgccgcgac atagcccggg ttcagccgga gcgcctgttc
     8161 gaagtggcgg attgcttccg cgtgttcgcc cccctgcatc aacgtggtcc caaggttgta
     8221 ctgagcggcg gcagactcgg gctgcagccg cgccgactca cggaagtgcg ccacggcttc
     8281 ggccaccttg ccgaattgca gatagtgcag cgccacctcg ttgtgctcga tcacgagctc
     8341 gggatgagct gccagtatca tctcggagcc gacgatgtct tcctcgagtt cgcgacgcga
     8401 gcgatcgctg ttgagggtct ggagatcggc ggccgtgcgc ggcaagacct gaatccacag
     8461 gtcacccatt tcgttcgacg actgctggcc ccatgtcacg cgtgtcggcg gtgcgttcgg
     8521 attgcgccga ttgccggcgg agttgtcgta ggtgatctcc atcaccagct cggtgccgcg
     8581 gggcaggacg aacggcttct cgtattcgta gaagtcctgc cagtcgaaat cccagtcctt
     8641 gatcgcgatg agccatttcc gcgtcccgtc gggcagcgtc gcgtacccct tgatgtctct
     8701 ggccaggtaa tgcgcgtgcg gctggacgcc gagcacctcg acgtccaccg gcagcacgta
     8761 gctgtcgcgg ctcacatggg ccgtcgctcc cgccgggatg tcgagattct ggcgacccag
     8821 tctgatggag gccgggatgc gagtgggtac cgtgtcggcg aagtacagtc cgatacggcc
     8881 cttcacctgc tcgggccgcg agcccggcat caggtggagt tcgagcagca tgtcggaccc
     8941 ggcgtcgacg cgccacgcca tggtatcggg cgccaatggt cgtagctgcc cgggtgtcca
     9001 gccgaggaag tagcctggcg gaaaccgcgc cgcgaatggc gtcacgccct catagcctga
     9061 cgcgggatcc gcggcgtcca acgtgcgcga ggcggcggtc ggatcgatac gcatgttcgc
     9121 gtggtgcacg actctcgcgt tcccgggctg gaattccaca cccttcacat accgggtgcg
     9181 gtcgatggga atggggatgg cgaacttgcg gaggacgtct ggcccgcccg cgggaagcgg
     9241 gtagggcgcc atctcgacga tgaggtccgg tgtgccgagg cgccacccac cgtcgggcca
     9301 gactggcgcc ggcgggagat cggctgcacg tccttcgatc gcaccctcag ccacccagcg
     9361 ctcgatgagc gcgaccagtt cgtcaggcag ccggcgatcg ccgatgaacg gaccgccgtg
     9421 ttctggttcg gccttccacg gtggcattct gcgggcgctg acctgctcgc ggatggctcg
     9481 cgcccacggc ctgacctcct gatacgagag cagggacatg ggtcccaacg cgccaggccg
     9541 gtgacacacc gcgcagtgtt ggtacaggat gggggcgatg tcgcgggcga aggtgagagg
     9601 ctcgccaatg cgctgacccg cggcaccgat ggtcgcctgg gccgcgaggg ttcccgtcgc
     9661 gagtgctcct accgccagac acactcccat ccggaggatc gatcgctgtg cgccgacgcg
     9721 tgtcgaacgg ctcatgcgga gagtccactc gctacaacgg gttcggatcg agcaggttcg
     9781 gatcgacgac gggtcctggt ctgtactccg ggtcgtcgtc tcgccacggc gtgcgacacg
     9841 gctcgtgtcg ctggtagagc gccagattcg cggccatccg cttcgcggca tcggagtgac
     9901 cggcgtcggc ggcgaccgac atggccaggc gctgccattc gacggcgtcc tcgaagcggc
     9961 caacctccgc cagcgccatc gccatcgttt ccgccacgct cgtggtcttg tgctggttgg
    10021 ccagcgacgt gacgagatcg agggccagcc ggccgtcccg cacctgcggg tcaggagaag
    10081 ccgccagcag gcgagccaat gcctgagcga aggccggttg atccggatga cgcatcatgg
    10141 cgtcgcgtag acggcctcgg gcctccgcgt ggcgcgacag ctgcgccagg gccatcgctt
    10201 cgccgaatcg cgcgtcctcg gcatccgggg cgagctcgag cacgcggcga tacgacgaca
    10261 gggccgcgtc cagccggccg acgcggcgca gcgcgtcggc gagtcggacg tgcgcctcgc
    10321 gcaacgtcgg gtcgtgtgtt gcggcggctg tgtactctgc gatcgcgcgc tcgtcgcggc
    10381 cgccccgctc gaggagaacc gccttcaaga gatgcgccgc cgcgagcgaa ggatcgacgg
    10441 cgatcgcctt atccagttct gacatggcgg cgcgagcatc gcccagacgg ttcaacgtct
    10501 gggcgagtcc gacgcgatat cccgcgttgg ctggcgccag atcgatcgcc ttgcggaacc
    10561 cggtggccgc ttccagccac tgtcctctgg cgctggcctg ccgagcgagt tccgcgaaga
    10621 cgtcagggct tcgcagaatg gtgttcagct ccgccatgag cggatcggcc atcgtcacgg
    10681 ttgcgccggc cccgccgcgt ccagctattc gatcgaggtt cgactgggcc ttgtcgagat
    10741 cgccgaggcc acggtaggcc atcgcgagcg gttggtgaat gactgccgcc gccgggttca
    10801 gccgcagggc ttgctcgagg tatgtcacgg cactggcgaa gtcccgtttc gccaggctgg
    10861 cccgaccgag ttgataccac acagccgcgg cctcgggagc gagggcgcga gctctgttca
    10921 ggaacggttc cgccaaagcc ggttgaccca actcgatgtg gacatgaccg agccagacca
    10981 gagtcgcgaa atcgtcgggt cggagttcga gggcttgctc gagaggcgtc accgcctctg
    11041 tgagcgcccc cgcggtgatg aagaggtggc cgaggtagta cggccagcgg cgatcgctcg
    11101 gagcgagagt ctgcgcattc cgaaggcagc gttcggccac gtccggatac ctggcagcca
    11161 tcagcagctt gccaagcgtg ccgtaggcct cgccgcgctc gacttgtcgc gatgccgagc
    11221 gccggcggcc tccgtcgcgc ccggacggca acagattctg gagcgctgcg taggcatcgc
    11281 ggagctgctg ctgcacggac gggtgcatgc cagtgaggtc gggcaggttg acgggcagca
    11341 gcgccggttc gccggcggcg ccctgcatcg gggcatatgc cgtcacacga ggcacatgtg
    11401 agacgaggcg tcgcagactg gactgcttcg cgtgggccgc gagcggcgcg atcgccgcgc
    11461 acacgcacac gcaggcgagc agcgcggacg tcaccacgcc agcgctcgag aggccgcgcg
    11521 ccatactgcg gcccttcact atccgccgcc cccgggccga cggccgctgc cttccttcaa
    11581 tgtggtccag cggccgacgc cgacatccgt ccactcttcc tcccgcccgt tcggccacac
    11641 cacgagaacg cgccgaatcg ttgcggcatt gccaaggccg accaggacgc gtgggtcgtt
    11701 cgccgaggcg taactgccgt cggctcgcgc ccggcgccag agcggcgggc cctggctgcg
    11761 aaagacgccg accttcgcgc ccagcatatc tctgccccct gttcctccga ccaggcgcag
    11821 accaagccaa cggccgcgct ccccgacgtg gttgatcaac aggcgcgcgg gaccgttatt
    11881 gttcgtcacc aggacgtcga tgtcgccatc gttgtcgacg tcgccgaatg ccgctccgcg
    11941 gctgacctcc gacagctcga acacggcacc ggctcgggtc gagacgtcct cgaaccgcgc
    12001 gttgcccagg ttgcgaaaca gctggttgcg ctcatctaac ggaaacggag cggccactcg
    12061 cccagcacgt tcctggatct tcaccgagcc gttggcgacg aacaggtcca gccagccgtc
    12121 gttgtcgaag tcgaaccacg ccgtaccgaa tcccgtgtgg ggcatgctcg ggatgccgag
    12181 accggcccgt gccgtctgat catcgaagag gccggagccg tcgttgacgt agagggtgct
    12241 ggactctcct ggaaggttcg ttacgaacag gtcctcgtcg ccatcgttgt cgacgtcgcc
    12301 agcgtcgact cccatgctgc cctgggcgct gccgaggccg ttcagcgccg ctccggccag
    12361 aagcgccgtg tttcggaagc gaccgttctg ctgattcatc cacaggaagt tcgggaatcc
    12421 gtcgttggtc acgtagatat ccgcccaccc gtcggcattg aagtccgcgg cgaccacgcc
    12481 gagcgccgga cccgattcac gccccacttg tgcctcggcg gtcacgtc
//
LOCUS       DATLCO010000366         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6600190, whole genome shotgun sequence.
ACCESSION   DATLCO010000366 DATLCO010000000
VERSION     DATLCO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6600190"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VMZ33_08440"
     CDS             complement(<1..867)
                     /locus_tag="VMZ33_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HUR17299.1"
                     /translation="MRRRVVVTGMGVVTPLGNDVDTFWRRLVAGESGVRTIQNFDPSR
                     VTSTVAGEVVDFDPSQVLDRKEIRRNDRYTQLALVATREALGRAGLPERLEGELADRT
                     GTMIGSGIGGSGTLYEQMSINATRGPDRLSPFFIPMAIANMASGQAAISFGMRGPNVA
                     LVSACATAGHAIGEAMETIKRDDADMMVAGGSEASVYEPLVGAFAAMRALSTRNDDPE
                     GASRPFDQGRDGFVIAEGAGILVLEELDHARRRGARVLAEVLGYGATADASHITLPAA
                     GGEGGARAARRAL"
     gene            complement(893..1711)
                     /locus_tag="VMZ33_08445"
     CDS             complement(893..1711)
                     /locus_tag="VMZ33_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUR17300.1"
                     /translation="MATRLAVISDIHGNIAALEAALGDMERQRPDHILVCGDLFMHGP
                     HPVEVADRLRALEKDGAKVIAGNTDIAVADFDYMAAFPWMEDVPAASRAAIEWAHEQL
                     SDDQLDWLRRLPSERRVWADDVLVLACHGSPGSQTNGLPADMDVGVLVERVTRTDARV
                     IACGHTHVADVRELGRKLIVNPGSCGYAFDGDPGACWAMITVPDQPPAAAAENEDEDE
                     DGFGMVEVVDDTRPSAELFRPRYDAMAAADEVARRGLPGDVYRAATIRNGRQIS"
     gene            complement(1711..>2029)
                     /locus_tag="VMZ33_08450"
     CDS             complement(1711..>2029)
                     /locus_tag="VMZ33_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HUR17301.1"
                     /translation="EWFFQGHFPGLPVMPGVLQVEALAQTMAIYVAQQEGFGDRIGLF
                     AGIDGCRFKRTVLPGDRLTLEVTMEKLGKRFGRGKAMASVDGELCCEATISFIIPSVG
                     QLG"
BASE COUNT          336 a          714 c          661 g          318 t
ORIGIN      
        1 gagcgcgcgg cgagcggcac gcgcaccacc ctccccgccc gctgcgggca gcgtgatgtg
       61 gctcgcatcg gcggtcgcgc cgtagccgag cacctccgcc aggacgcgcg cgccgcgtcg
      121 acgagcgtgg tcgagctctt cgaggacgag gatgccggcg ccctcggcga tgacgaagcc
      181 gtcgcgaccc tggtcgaacg gtcggctcgc gccctccgga tcgtcgttgc gcgttgacag
      241 cgcgcgcatc gcggcgaacg caccgaccag aggctcgtac accgacgcct ccgatccacc
      301 ggcgaccatc atgtccgcgt cgtcgcgctt gatcgtctcc atcgcctcgc ctatcgcatg
      361 accggccgtg gcacaggcgc tcacgagcgc gacgttgggc ccgcgcatgc cgaacgagat
      421 ggcggcctgg cccgaagcca tattggctat ggccatcggg atgaagaatg gcgacaggcg
      481 atctgggccg cgcgtggcgt tgatcgacat ctgctcatag agcgtgcccg agccgccgat
      541 cccagagccg atcatcgtgc ccgtcctgtc ggccaactcc ccctcgagac gctccggcaa
      601 cccggcccgg ccgagggcct cgcgcgtcgc cacgagtgcg agctgcgtgt agcgatcgtt
      661 gcgccgaatt tctttgcggt cgagcacctg ggaggggtcg aagtcgacta cctctccggc
      721 gacggtgctg gtgacgcgcg aggggtcgaa gttctggatc gtgcgcacgc ccgactcacc
      781 ggcaacgagc cggcgccaga acgtgtcgac gtcgttgccg agtggcgtca cgacacccat
      841 gccggtgacg acgacgcgcc gcctcacgaa cccaacctca agagcccaag gttcaagata
      901 tttgccgccc attccggatc gtggccgcgc gataaacgtc acccgggagg ccgcgcctgg
      961 ctacttcatc ggccgcggcc atggcgtcgt agcgtggcct gaaaagctca gcggaggggc
     1021 gggtgtcgtc cacaacctcg accatcccaa agccgtcttc gtcctcatcc tcgttctcgg
     1081 ccgcggcggc cggaggctgg tcggggaccg tgatcatggc ccagcacgca ccagggtcgc
     1141 cgtcgaacgc atatccgcac gagcctgggt tgacgatgag cttgcgcccg agctcacgaa
     1201 cgtcggcaac atgcgtgtgt ccgcaggcga tcacgcgcgc gtcggtgcgc gtgacccttt
     1261 ccacgagcac acccacgtcc atgtctgccg gaagcccgtt ggtttggctg ccgggcgacc
     1321 cgtggcaggc gagcaccagc acgtcatcgg cccaaacacg tcgctcagag ggcagccggc
     1381 gcagccaatc gagctggtca tcggacagct gctcgtgcgc ccactcgata gcagctcggc
     1441 tcgcggcggg gacgtcttcc atccacggga acgcggccat gtagtcgaag tcagcgaccg
     1501 cgatgtcggt attgccggcg atgaccttcg caccatcctt ttcgagtgcg cgcaatcggt
     1561 cggcgacctc cacgggatgc gggccgtgca tgaacaggtc gccgcacacg aggatgtgat
     1621 ccggacgttg gcgctccatg tccccgagcg cggcctcgag cgccgcgatg ttgccgtgga
     1681 tgtcgctgat cacggccagg cgggttgcca tcagccgagc tgcccgacgc tcggaatgat
     1741 gaacgagatg gtcgcctcgc agcagagctc gccgtcgacg ctggccatgg ccttgccccg
     1801 accaaagcgc ttgcccagct tctccatcgt gacctcgagg gtcagccgat caccgggcag
     1861 gacagtccgc ttgaagcgac atccgtcaat gcccgcgaac agcccgatgc ggtcaccgaa
     1921 gccctcctgc tgggccacgt agatcgccat cgtctgagcg agcgcctcta cctggagcac
     1981 gcccggcatc accggcagtc ccgggaagtg gccctggaag aaccactcg
//
LOCUS       DATLCO010000367         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14326 k141_6602641, whole genome shotgun sequence.
ACCESSION   DATLCO010000367 DATLCO010000000
VERSION     DATLCO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938805
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:46:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,692
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6602641"
                     /isolate="SMAG_U14326"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1175
                     /locus_tag="VMZ33_08455"
     CDS             246..1175
                     /locus_tag="VMZ33_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HUR17302.1"
                     /translation="MPMPHRTFAQVAKRRLVGKEGKERIREVRALMAELPDYKNGPYA
                     DLRKALLAEIEDTRVRSSAVHRDSIAVRREGAAQIALVGPPNVGKSSILQALSEIQIK
                     TGDYPFTTLRPVPALTRVHGVLVQLVEIPGLIGGATDDKGGGRALLGVLRSADAIVYC
                     ARPGHDPDELATVINEVEIAGIDKPGILAATRADEATAAEIDRLRAAFPEMRMVTVSV
                     IDTDSLERLRDEMWAMTGLIRVYLRANGSASAEPLALHEGATVTDVADSVHHDLGSSF
                     TAARIWGPSARFEGQRVGREHTVQDSDTVEILR"
     gene            complement(1184..2035)
                     /locus_tag="VMZ33_08460"
     CDS             complement(1184..2035)
                     /locus_tag="VMZ33_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HUR17303.1"
                     /translation="MASAMKVIAQLPLSYELASGGTTHSPLTYVSVGGLEPARFVLDT
                     GSDVHLFNEDLADELGLDKQPGEEGTDHSGATMPSWSVGDVPAAMGNVPLLLRDVIAI
                     PAPPPFPGFGIRGILSPQHLHPTAIAVMDMANDELLLVEASDEEVLDFLRDRLPGIEV
                     QRLGRDPDSGAVVVRAGIEGYPEVRAVLDTGGKETEFTVSAVPGLIAREMQRLGAGVS
                     GGGYEGGFVGPHTLLLGGARLSVPDLAVREQIHDVDAIVSMDALRGTVIAAAADLARP
                     VFLAKAD"
BASE COUNT          401 a          721 c          756 g          351 t
ORIGIN      
        1 agcgacgggc cgtcagggtc agattcccgc gacaggctcg catttcaacc aacgcactgc
       61 ctgggcaacc gttaccatcc gccggcgatg accgcctggt acgccaccat ctcacgacag
      121 aacggctgat cagccccttc ccaccaagcg tcaaaggaag gggcgggaca gacgcacgcc
      181 gcaagtcgca gccgataagg gctgcgcgcc gtgccgtccc gcgtgatcgt cgtaggagat
      241 gagacatgcc gatgccgcat cggacgttcg cccaggtggc gaagcgtcga ttggtaggca
      301 aagagggcaa ggagcgcatc cgcgaggtac gggcgctgat ggcggagctg cccgactaca
      361 agaacgggcc atacgccgac ctgcggaagg cgttgctggc tgagatcgag gacacccgcg
      421 tccgctcgag cgcggtccac cgcgactcga tcgccgttcg gcgcgaggga gcggcgcaga
      481 ttgccctggt cgggccaccg aacgtcggca agtcgtcgat cctgcaggct ctgtcggaga
      541 ttcagatcaa gactggcgac tacccgttca cgacgctacg tccggttcca gcgctgaccc
      601 gcgtccacgg cgtgctcgtg cagctcgtcg agatcccggg tctcattggt ggcgcgaccg
      661 acgacaaggg cggtggccga gcccttctgg gcgtgctgcg ctcggccgac gcgatcgtgt
      721 actgcgcgcg cccgggtcac gatccggacg agctggcaac ggtcatcaac gaggttgaga
      781 tcgccggcat cgacaagccg gggatcctgg cggcgacgcg cgccgacgag gcaaccgcgg
      841 ccgagataga ccgcctgcgg gcagccttcc ccgagatgag gatggtgacg gtgtcggtga
      901 tcgataccga cagcctcgaa cgactgcgcg acgagatgtg ggcaatgacc ggcctgatcc
      961 gcgtgtacct gcgcgcgaac gggtcggcca gcgctgagcc gctggcgctc cacgaagggg
     1021 cgacggtcac ggacgtggcg gactcggtcc accacgatct gggctcgagc ttcacggcgg
     1081 caaggatctg gggaccatcg gcgcgtttcg aggggcagcg cgtaggacgt gaacacaccg
     1141 tgcaggacag cgacacggtc gaaatcctgc gctgaacgct gtgtcagtcc gcctttgcga
     1201 gaaagacggg gcgcgcgagg tctgcggctg cggcgatgac cgtgccgcgc agggcatcca
     1261 tgctgacgat ggcgtcgaca tcgtggatct gctcgcgtac cgcgaggtcc ggcaccgata
     1321 ggcgcgcgcc gccgagcagc agcgtgtggg gcccgacaaa gccaccctcg tagccgccac
     1381 cgctcacgcc agcacccaga cgctgcatct cccgcgcgat gaggccaggc accgcgctga
     1441 cggtgaactc cgtctccttg ccgccggtgt cgagcacggc tcggacttcg ggatagccct
     1501 cgatgccggc tcgcactacg accgcgcccg aatcgggatc ccgcccgagc cgctggacct
     1561 cgataccggg aagtcggtcg cgcaggaagt cgagaacctc ttcatcggac gcttcgacga
     1621 gcaaaagctc gtcgttcgcc atgtccatca cagcgatagc ggtcggatgc aggtgctgcg
     1681 ggctgaggat gccgcgaatg ccaaagcccg gaaacggtgg cggggctggg atcgcgatca
     1741 cgtcgcgcaa caagagaggg acgttgccca ttgccgccgg tacgtcgccg actgaccaac
     1801 tcggcatcgt cgcgccgctg tggtcggtgc cctcctcgcc cggttgcttg tcgagcccga
     1861 gttcgtccgc caggtcctcg ttgaagaggt gaacgtctga accggtgtcg agcacgaatc
     1921 gtgccggctc caggccgccg accgagacat acgtcagtgg tgagtgcgtc gtgcctccgc
     1981 ttgcgagttc gtagctgagc ggcagctggg caatcacctt catcgcgctt gccatattcg
     2041 tggaatcaaa aaccgcccgc atcgctgcgg gcggtttgct taggcctcgg aaggcttagg
     2101 cgctctgaac tcgacgtgcg cgcgggccct tggggctctg ctcgatctcg aattcgacct
     2161 tctcgccacc gaccaaacgg tcgaagtcga gcgaggtgtc aagggcgtcg cggtggaaga
     2221 aataatcct
//
